LOCUS       DATIIX010000001         3214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_472138, whole genome shotgun sequence.
ACCESSION   DATIIX010000001 DATIIX010000000
VERSION     DATIIX010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472138"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            721..1758
                     /locus_tag="VLF64_00005"
     CDS             721..1758
                     /locus_tag="VLF64_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90363.1"
                     /translation="MLHTPGGGSNDILHRNLEPYLQSHVTNDQALVTWKEGPRTGETY
                     DSVALAECDFSTTPSIEHIEEQIVERFAEHQAKDISPDTYIPNERERVWRAVGEAITI
                     LYKAYYGTPSLGNGIELAQMFDLDGKGEAYNMFSNKIIKDGRRAYLAELGLRHDIRGL
                     DISHGDSMTGVHMASDLCFAGDGDLAEYSSMALTHSTVTDITPAIIQMKALACDDARA
                     RANLSKMSGENKRGRASGGEYVFFWPDDSVTETQPFTKAFDPYAGLELPAVWGIKNTK
                     PMEDAGLVIKNGHWQNEKRVAGAVPLEFYSNLWVPPMAVERHTKLLTSHGFGHIQVSP
                     FPKLLSRRTAT"
     gene            1857..>3214
                     /locus_tag="VLF64_00010"
     CDS             1857..>3214
                     /locus_tag="VLF64_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90364.1"
                     /translation="MKIKNVVGTAVSLALAFVALSASHPVYAAGANCTWTGAVNTSWN
                     TAGNWSSCNGTRPQNGDNLIFDTTSLASNKILNNNISNLSVGSITFTGGGSHNYTVMG
                     NDITLSNGFSDSTCNFNSLDIGITLSADQSFTATGCSPSIGTSSKDLNIGSYHLTLNG
                     SQGIDMESPFVGDGTITISTTTHPFTFFDADSPNFSGHINISDTRLIFNVAQPDALGT
                     ADITINDDAVLQESYNTSNSYTFDNDITLIGDGGGNNGAINLHDFTGSGTSTLTFTGK
                     VTLTGDATIDLDNANAIFLSRPVGCGYTITKSAGSGGSGGSLIGNLTGSCPATSTDNY
                     TWTKQTSAPSTDWQGIAGSSDGKYLVSAVWSGDILTSNDYGATWVDRSSAGSHNWVGV
                     ASSADGAHLAATAYGGDIWTSSDYGVTWVDRSSAGAHDWNFLASSSDGSHLAATANGG
                     DI"
BASE COUNT          865 a          785 c          796 g          768 t
ORIGIN      
        1 atcacagctg ctggcagtga tcataacgtg gctgataaaa actacattgc agccatagta
       61 gaagctatct agtgttatat tgcaaatatc tggactacag aagcatcgac atcactggag
      121 atgttcatgc actatacact acccagggtg atacgattat tatctttggc ttcattagta
      181 cacacagtca gctatattaa gacctgtacc gcactcaatt tcagttgtga taagatgcac
      241 gccgggcctc cacgcataag acttcgacgc ttagttttct gctgatacaa ttgcagtatg
      301 acgaatttta agttgcttat atgcaaaact taagcgacct gttaagtcta gaggaaataa
      361 agcgcctgtc cctgccctct aactttgcat atggcaaggc tatatgtgac cggggagcag
      421 tggagcttat acataaagag ccagctgcag cagaagcctg ggtcggggga ctggatggaa
      481 cggttattga aggagctggc tcacgccgcc gtacacagtt atatgtatcc ccaaaaggtc
      541 tggcgtggca ttgtactggc aaccccaaaa atcacgatat tttttgcaag cattgcgtag
      601 cgttagcgct tgccctcctt ggcgatcgtt agcctatctg catgtatagg ttcaattgcg
      661 atagcgtgca cgatggtcct catacttgca actacactag cattatatta ttaattatct
      721 atgttacaca ctcccggcgg cggttcaaat gacatactac accgtaacct agaaccatat
      781 ctacagtctc acgtaacgaa cgaccaggcc ctcgttacct ggaaagaagg accacgaacc
      841 ggcgaaacat atgatagcgt agcactagcc gagtgcgact tctctactac cccgagcatc
      901 gaacatatag aagagcaaat cgtcgaacgt tttgcagaac atcaggcaaa ggatattagc
      961 cccgatacgt acatacctaa tgaacgcgag cgagtatggc gagcagtagg cgaggctata
     1021 actattctgt ataaagcgta ttatggcacc ccttctttag ggaacggcat tgagctggca
     1081 caaatgttcg acctggacgg taaaggtgag gcatacaata tgttttcgaa caaaataatc
     1141 aaagatggac gacgagccta tcttgccgag ctcggcttgc gccacgacat tagagggcta
     1201 gacatatcac atggcgattc gatgactgga gtacatatgg caagtgatct ctgcttcgca
     1261 ggagatggtg atctcgctga atattcgagc atggcactga cacatagcac tgttaccgac
     1321 attactccgg caataatcca aatgaaggcc cttgcatgcg acgacgctag agcaagggcg
     1381 aatcttagta aaatgtcagg cgaaaataaa cgcggccgag ccagcggagg cgaatatgta
     1441 tttttctggc cagatgacag cgttaccgag actcaacctt ttacgaaggc ttttgaccca
     1501 tacgcaggtc ttgagttacc tgcggtctgg ggcattaaaa atacgaaacc tatggaagat
     1561 gcaggcctcg taatcaagaa cgggcattgg caaaatgaaa aaagggtagc cggagcagtc
     1621 cctctggagt tttattcgaa cttgtgggtt ccccccatgg ctgtcgaacg acataccaaa
     1681 ttgcttacct cccacggctt cggccatata caagtctcgc cttttccaaa gcttctttcc
     1741 cggcggactg ctacctaata gacaagcaaa gcgtctccga ttcccttatc caaaacctgt
     1801 gcatactgta tattgatata cagaaccgtt ttattagcag tggtggtgga agtgctatga
     1861 aaatcaaaaa cgttgtaggg acagcagtat ctctggcatt ggcttttgtt gcactaagtg
     1921 cgtcccaccc ggtatacgca gctggcgcaa actgtacctg gaccggcgca gttaacactt
     1981 cttggaacac agccggcaac tggagcagct gtaacggcac cagaccccaa aacggggata
     2041 atctaatttt tgacaccact agtcttgcaa gcaacaagat cttgaacaac aatatttcaa
     2101 acttgtcggt gggctctatt acgttcactg gcggtggatc gcataattat acggtcatgg
     2161 gcaacgacat aaccttgtcc aacgggtttt ctgattctac atgcaacttt aactcgttag
     2221 atattggcat taccttatcg gccgaccaaa gttttaccgc aacgggatgt tccccgagca
     2281 ttggcactag ttcaaaggac ttaaacattg gtagctatca cctgacgctg aacggatcgc
     2341 aaggtataga catggaatcg ccttttgtgg gggatggaac gataacaatc agcacaacca
     2401 cccacccatt caccttcttt gatgccgata gccctaactt cagcggccat ataaatatca
     2461 gtgacacccg gttgattttt aacgttgccc aaccagacgc tttaggcacc gccgacatta
     2521 cgataaacga cgatgccgta ctgcaggaaa gctataatac ctcgaacagc tacacattcg
     2581 acaatgacat tacgttaatt ggtgatggcg gtggcaacaa cggcgccatt aacttgcacg
     2641 actttaccgg cagcggcacc agcacgctga cttttaccgg aaaggttacg cttacgggcg
     2701 acgctacaat tgacctagat aatgcgaacg caatattctt gagccggccg gttggctgtg
     2761 gctacacgat tactaagtcc gccggatcgg gcggcagcgg cggatcgctc attggaaacc
     2821 tcactggcag ctgcccggcg accagcacag ataattacac ctggacaaag caaacgtcag
     2881 cgccgtctac cgactggcaa gggattgccg gatcgagtga tgggaaatac ctggtcagcg
     2941 ctgtttggag tggtgatatt ttgacgtcga acgactacgg tgcgacctgg gtggaccgga
     3001 gttcggcggg ctcgcacaac tgggtgggcg ttgcctcttc ggcagacggg gcgcacttgg
     3061 cggcgactgc ttacggcggg gatatatgga cttcgagtga ctatggggtt acctgggtgg
     3121 accggagttc ggccggggct catgattgga acttcttggc ctcctcaagc gacgggtcgc
     3181 atctagcagc gaccgccaat gggggtgata tttg
//
LOCUS       DATIIX010000002         2438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_111250, whole genome shotgun sequence.
ACCESSION   DATIIX010000002 DATIIX010000000
VERSION     DATIIX010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111250"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VLF64_00015"
     CDS             <1..239
                     /locus_tag="VLF64_00015"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HSW90365.1"
                     /translation="GESVQDEFMLLVEEVGELAKAMRPLNGITYATDSQKLELEHEAA
                     DVFWLLTCVCNSLGIDLAEAVRSKEAKNNTRIWK"
     gene            249..902
                     /locus_tag="VLF64_00020"
     CDS             249..902
                     /locus_tag="VLF64_00020"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HSW90366.1"
                     /translation="MKVTGLSSQQKDPNRINILVDGKFRFSLDISQVTDLGIRVGKEF
                     TEEKLDEFEVESQFGKLYARALEYCIMRPHSAREVRDYLYRKTRTTQVRNKHTGKVKE
                     RPGVSIELTTRVFERLLDKGYIDDTKFTHWWVENRNQRKGVSRRKLQSELSAKGVASG
                     IIEEAIASTERNDGDELAKIVSKKRAKYPDNQKFMQYLTRQGFSYDDIKSALNKGDD"
     gene            complement(899..1486)
                     /locus_tag="VLF64_00025"
     CDS             complement(899..1486)
                     /locus_tag="VLF64_00025"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HSW90367.1"
                     /translation="MMLLGSTLTKAPVMGLQTGGEIAQTKSAIIDPATLAIIAYIVEG
                     SSLEDGIWLLRIADVRELSDLGFIVDSTDEFIHPEDVLKVNEIYNLNFSLLDMPVVDE
                     KRSKLGKVIDFTLETSTFTIQQLTVRRPLFKSFNDTELLIHRSQIIEINDDTIVVHSE
                     AKAPEPELHEVVGSYVNPFRKSNSPTPESSDTKAS"
     gene            complement(1494..1772)
                     /locus_tag="VLF64_00030"
     CDS             complement(1494..1772)
                     /locus_tag="VLF64_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90368.1"
                     /translation="MKSDLSLQQISHSLVHFLHRYHVLIFTFFVLGGLSAATFMLYRV
                     TTDAQSTNSTTPSSFDQKTIDRIDKLHSANDVPTPINLPAGRTNPFQE"
     gene            complement(1772..2392)
                     /locus_tag="VLF64_00035"
     CDS             complement(1772..2392)
                     /locus_tag="VLF64_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90369.1"
                     /translation="MKLKNMTPVKLRIILALSLIILSILGIGMFAFGYGQLKLFVIAA
                     QDVAAKAQSSQSSVQDLVATKKFLQSNADAINRANQLAAESMSYVYQDQVINDINKYA
                     SEAGLAITNIAFTTPTTTAVGGTTAPPVTGSAATTAAPAGVKSMTANVTIKNPTNYLA
                     MLDFIHLIEQSLFRMQISQIGISASSDDKNPNQVSSDILTIEVYVR"
BASE COUNT          686 a          503 c          610 g          639 t
ORIGIN      
        1 aaggcgaatc agtccaggat gaattcatgt tgcttgtcga agaagttggt gagctggcaa
       61 aggcaatgcg tccgctcaat ggtatcacct atgcaacaga ttcacagaag ctagagcttg
      121 agcatgaagc tgctgatgta ttctggctac ttacgtgtgt ttgcaatagc cttggcattg
      181 atctcgctga ggcggtgcgt tcaaaagagg cgaaaaacaa tacacgcatc tggaagtaag
      241 agttacatat gaaggttacc gggctctcat cgcaacaaaa agacccgaat cgcatcaata
      301 ttttagttga tggcaaattc agattcagcc tcgatatttc ccaggttacc gacctgggaa
      361 ttcgagttgg caaagaattc acagaagaga agctagatga atttgaagtc gagagtcaat
      421 ttggtaagct gtatgccaga gctcttgaat attgtattat gcggccgcac agtgcacgtg
      481 aagtacgaga ctacttgtat aggaagacgc gcactactca ggtacgaaat aaacatacag
      541 gtaaggttaa agaacggcca ggtgtgagta tagagctcac cacacgcgta ttcgagagac
      601 ttttggataa aggctatatc gacgatacca aatttacgca ctggtgggta gaaaatagga
      661 accagcgtaa aggtgtaagt cggcgcaaac tgcaatctga attgtctgcg aagggagtgg
      721 cctctggcat catcgaagag gccattgcgt caacagagcg caatgatggc gatgaacttg
      781 caaaaatcgt aagcaaaaaa cgagcaaaat accctgataa ccaaaaattt atgcaatatt
      841 taacgcgcca agggtttagc tatgacgata tcaagtctgc gctcaacaag ggggatgatt
      901 aagaggcttt tgtatcactc gattcaggcg ttggcgagtt agacttacga aacggattaa
      961 cgtagctgcc aacgacttca tggagttctg gctctggcgc cttcgcttcg ctatgcacga
     1021 cgatagtatc atcgttgatt tcgataatct gagatcgatg aatgagaagc tcggtatcat
     1081 tgaagctttt gaataatggc cggcgaacgg tgagctgctg tatggtaaat gtgctagtct
     1141 cgagcgtgaa gtcaataacc ttgccaagct tgcttcgctt ttcgtctacg acaggcatgt
     1201 caagcagtga gaagttaagg ttataaatct cattaacctt aaggacatct tctggatgaa
     1261 taaactcatc tgtagaatca acaatgaagc cgagatcact gagctcacgc acatcggcga
     1321 tacgaagcaa ccatatcccg tcttcaagtg atgatccctc tacaatgtag gcgataatag
     1381 cgagcgtcgc tgggtcgatg attgcagact ttgtttgagc aatttcacca cctgtctgca
     1441 ggcccatcac tggcgcttta gtgagcgtgg atccaagaag catcatatgt tgattactcc
     1501 tggaacgggt tagttcggcc cgctggtaaa ttgattggtg ttggaacgtc atttgcactg
     1561 tgaagtttgt caatacgatc aatcgtcttt tgatcaaaac tacttggtgt agtactattt
     1621 gttgactgcg catcagtggt tactcggtac agcatgaatg ttgcggctga aagcccacca
     1681 agtacaaaga aagtaaaaat caaaacatga tagcgatgca aaaaatgtac caagctatgg
     1741 cttatctgtt gtagggagag atcggacttc attagcgtac gtatacctcg attgttagaa
     1801 tgtcgctact gacttgattc gggtttttat catcactgga agcagagatg ccaatctgag
     1861 aaatctgcat acggaacaga ctttgctcaa taaggtggat gaaatcaagc atcgcaaggt
     1921 aattagtcgg attttttatg gtaacatttg cggtcattga ttttacaccc gccggtgctg
     1981 cggtggtcgc agccgatccg gttacagggg gcgccgtagt gcctccgaca gccgtagtcg
     2041 tcggggtggt gaatgcaatg tttgttatag ccagacccgc ctcgctagcg tacttgttaa
     2101 tgtcattaat cacctgatcc tgatacacgt agctcatact ttcggccgcc aactggttag
     2161 cgcggttaat agcgtccgcg tttgattgca ggaacttctt tgtcgcgaca agatcctgca
     2221 cgcttgactg gcttgactgg gcttttgccg ccacgtcctg tgcggcgatg acgaatagct
     2281 tgagttgacc atatccgaaa gcaaacatac caatgcccag aatggacaaa ataataagac
     2341 tcagggcgag gataatgcga agcttgactg gtgtcatatt tttcaatttc attgtgccag
     2401 tgccgctttg gaataagtta cattatatat tgcagtga
//
LOCUS       DATIIX010000003         5794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_333819, whole genome shotgun sequence.
ACCESSION   DATIIX010000003 DATIIX010000000
VERSION     DATIIX010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5794
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333819"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VLF64_00040"
     CDS             complement(<1..237)
                     /locus_tag="VLF64_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HSW90370.1"
                     /translation="MEIVYLVTVLGVILVSMMLHELAHGFIALKLGDETARLLGRLSF
                     NPLKHIDPYMTIGLPMMIVILNLLTGANMPVFGGA"
     gene            338..538
                     /gene="rpmB"
                     /locus_tag="VLF64_00045"
     CDS             338..538
                     /gene="rpmB"
                     /locus_tag="VLF64_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HSW90371.1"
                     /translation="MAARCELTGKGKQFGHNVSFSLRRTKRVFKPNLQKKTFVVDGQK
                     ITMTLSTQAIRTLKKKGILATA"
     gene            complement(617..1252)
                     /locus_tag="VLF64_00050"
     CDS             complement(617..1252)
                     /locus_tag="VLF64_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSW90372.1"
                     /translation="MFDLEYKGGNTVVFSTKKVSLTTDPKLSLVGLKDIKTKDEVELA
                     TETRFSVKNPDARIIIDSPGEYEVSDFTIHGIAATRHIDTPDQEKLSTIYRIECGDVK
                     VAVLGNIDAKLNEDQLEAIGVVDVLILPVGGGGYTLDATSAAALTRSIEPKAVVPVHY
                     ADSALKYEVPQEELALFVSELGVPVETMTKYKIKGNSSLPGALTIIELIRS"
     gene            complement(1266..1853)
                     /locus_tag="VLF64_00055"
     CDS             complement(1266..1853)
                     /locus_tag="VLF64_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HSW90373.1"
                     /translation="MIDALFGSKTRVKLLHLFLNNPGKAFYVREITRLIGEQINSVRR
                     ELSNMLDVGIITSKNTDNKLYYEVNQRYDFYVPLRTIFADQKSEPLKKDSKSSEWQKA
                     IKDLAGLRVAVIAGMLVDGSASGVDLLLVGDLSEKKIQAAIHAIESAEGRELAYTILT
                     YDEFYYRLSVHDRFINDILNNKHAILTDVDEILSK"
     gene            complement(1883..2779)
                     /gene="miaA"
                     /locus_tag="VLF64_00060"
     CDS             complement(1883..2779)
                     /gene="miaA"
                     /locus_tag="VLF64_00060"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HSW90374.1"
                     /translation="MATNRFRSEPPLVLIVGPTASGKTSLGIKLAKHYGGEIISADSR
                     AIYKDLSIGTAKPSLKEQDGIPHWGIDLVTPGERFTAADFQQYASEKIAEIRARDHVP
                     IIVGGTGLYVDAVMYDFEFPGEANDVRRRESLMMQSLDELYEYCSINNITLPENKKNK
                     RYVVNNILRNGHFLKRKHELDGNTIVVGISTERDILRSRIEGRAAAIFRDGVIEEAQK
                     AAAVYGWDNEAMTGNIYPLIHYYLDGQLTRDELEQKFIVRDWQLAKRQLTWLKRNEHI
                     YWADLDNAYTYLTRRLDELSNL"
     gene            complement(2737..3087)
                     /locus_tag="VLF64_00065"
     CDS             complement(2737..3087)
                     /locus_tag="VLF64_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HSW90375.1"
                     /translation="MDNLEYEKNIRERVYQLMRDLPADKVTTYGDLAAMAGSPRAARI
                     VGGIAHQGPEDLPWHRLVNAQGGLAVGFHGGQVVQRQLLEQDGIECDENWHVCNFGER
                     RWRPIDFDQNLRLF"
     gene            complement(3088..4899)
                     /gene="thrS"
                     /locus_tag="VLF64_00070"
     CDS             complement(3088..4899)
                     /gene="thrS"
                     /locus_tag="VLF64_00070"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HSW90376.1"
                     /translation="MNEEQLYAMRHSLAHITAQAVQHLWPQAKFGVGPVVENGFYYDI
                     DLGDTKISEDDFKKIEKEMRGIIARNFPFERSEKNVDEAIDWAKEHSQPYKEELLNDL
                     RRTGTTIAKDLDAAELGLAADGDVAVEQVSFYTDGDFTDLCRGPHAESTGKVGAFKLM
                     RVAGAYWRGNEKNPQMQRLYGVAFATKEELDEHLKMLKEAKARDHRRLGKELDLYTMS
                     PLVGAGLPLFTPRGTVLREKVAAFSNQLREARGFTKVWTPNITKIALYETSGHWAKFG
                     DELFLVTSQETDDKMALKPMNCPHHTQIYASQPRSYRDMPVRFLETTTDYRDEKTGEL
                     GGLNRVRSLTQDDSHVFARPDQIEQEIDGLLASARELYQTIGMQLRVRLSYRDDGDGY
                     LGDIDLWQSAQAQLKSAVEKNQLEYFEQEGEAAFYGPKIDFMATDAIGREHQVATVQL
                     DFVQPGRFGLVYAAEDGTKQQPIMIHCALLGSIERFLSVFIEHTGGWFPFWAAPEQVR
                     ILTINDAVKPYVDEIASLLSGLVVMKPVKYNEVRFTTDDRNESLGKKIREATAMKIPV
                     QLIVGPKDMEAHEVSVRTQKGEEKVALNALADYLKAL"
     gene            5187..5263
                     /locus_tag="VLF64_00075"
     tRNA            5187..5263
                     /locus_tag="VLF64_00075"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5221..5223,aa:Val,seq:gac)
     gene            complement(5609..>5794)
                     /locus_tag="VLF64_00080"
     CDS             complement(5609..>5794)
                     /locus_tag="VLF64_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90377.1"
                     /translation="MVLQWILMPVTSICYSAIASLNAQTHLMLGKYLDKFDVTDKATM
                     ASRERHKELMKQKKSDA"
BASE COUNT         1596 a         1496 c         1107 g         1595 t
ORIGIN      
        1 agcgccacca aacaccggca tattcgcccc cgtcaaaaga ttaaggatga caatcatcat
       61 cggaagtccg atcgtcatgt acggatcaat gtgtttgagc gggttaaacg acaatctccc
      121 cagtagtcgc gccgtctcgt cccctagctt taatgcgata aacccgtgcg caagctcatg
      181 gagcatcatt gagacaagaa taacgcctaa aacagtaaca agatatacga tttccattcc
      241 ccctattgta gcacacgcta ccccttgacg aagatgcttg atgagcgtat actataaagg
      301 attgttataa aattaagtgt aagtggaact atcaactatg gcagcacgct gtgaactcac
      361 cggtaagggc aaacaattcg gccacaatgt cagcttctct ttgcgccgta caaagcgcgt
      421 ttttaagcca aatcttcaaa agaagacatt cgttgtagac ggccagaaga ttactatgac
      481 cctttctact caagctatcc gtactctcaa gaaaaagggt atcctagcta ccgcttaata
      541 agtactgctt attaagcaac aaaataccgc cgcagagttg tctgcagcgg tattttttga
      601 gtagcttact tgcacactaa ctacgaataa gttcaataat agtaagtgca cccgggagag
      661 acgaattccc cttgatctta tacttagtca ttgtttcaac tggtacaccg agttctgaca
      721 caaatagagc taattcctcc tgaggtacct catatttcaa tgctgaatca gcatagtgaa
      781 cgggaactac agcctttggc tcaattgagc gcgtaagtgc agcagcgctc gtagcatcaa
      841 gtgtatagcc accaccgcca accgggagaa taagaacatc aacaacccca attgcttcaa
      901 gttgatcctc gttaagcttt gcatcaatat taccaagtac cgcaactttc acatcaccac
      961 actcaatacg atatattgta gaaagcttct cctgatcggg agtatcgatg tgacgtgttg
     1021 ctgcaatacc atgaatagta aagtcactca cttcatactc acccgggctg tcaataataa
     1081 ttcgcgcatc tgggtttttt acactaaagc gggtttcggt agcaagctcc acctcatcct
     1141 tagtcttgat atccttaagc ccaacaagtg agagtttagg gtcggtcgtc aatgaaacct
     1201 tcttagttga gaaaactact gtattaccac ccttatactc taaatcaaac atattgttcc
     1261 ttaatttatt tacttaatat ctcatcaaca tcggtcaata tagcatgctt gttattgaga
     1321 atgtcattga tgaagcgatc gtgtacactg agcctataat aaaactcatc gtaggtcagg
     1381 atagtgtacg caagttccct gccttcagct gactcaatcg catggattgc agcctgaatc
     1441 tttttctccg acaaatcacc gacgagaagc aagtctaccc cgctcgccga tccatcaaca
     1501 agcataccgg cgataactgc aactctaagg cccgcaagat ctttaattgc cttttgccac
     1561 tcgctcgact tcgagtcctt tttaagtggc tcagacttct gatctgcaaa gattgtacga
     1621 agcggtacgt agaagtcata tcgttgattt acttcgtaat acagcttatt gtctgtattt
     1681 ttgctcgtaa tgatacccac gtcaagcata ttagaaagct cgcgacgaac tgaattaatc
     1741 tgttcaccaa taagtcgagt tatctctcgc acataaaagg ctttacccgg attattcaaa
     1801 aagagatgca gtaactttac acgagttttt gaaccaaata atgcatcaat catcccttat
     1861 atccccttct gtacgtatgg tatcacagat tactcagctc atctagtcgt cgagtaagat
     1921 acgtgtacgc attgtcgagg tctgcccaat aaatgtgctc attccgcttt agccaagtaa
     1981 gctggcgctt tgccaactgc caatctcgta caataaactt ctgctcaagc tcgtccctag
     2041 ttagctgacc atctaagtag taatgaataa gtggatagat attacctgtc atagcctcgt
     2101 tatcccaacc gtacactgct gcagcttttt gcgcctcctc aatcacacca tcgcgaaaaa
     2161 tagcagccgc gcgcccctca atgcggctcc tcaagatatc tctttccgta gatattccaa
     2221 caacaatagt gtttccatca agctcatgct tcctctttaa aaagtgccca tttcgcagta
     2281 tattattaac aacataccgc ttattctttt tattctctgg caatgtaatg ttgttaatag
     2341 agcaatattc atacaactca tcaaggcttt gcatcatcaa ggattcacga cgcctgacat
     2401 catttgcttc accaggaaat tcaaaatcat acataacagc atcaacataa agcccggtgc
     2461 cgccaacaat aattggcaca tgatcacgcg ctctgatttc tgcaatcttc tcgcttgcat
     2521 actgctggaa atcagccgca gtaaaccgct caccaggggt tacaagatca ataccccaat
     2581 gaggtattcc atcttgctct ttaagtgacg gcttagccgt acctattgat aaatctttat
     2641 atattgccct ggaatccgca gagatgatct cacccccata atgctttgca agcttaatcc
     2701 cgagtgatgt cttgccactc gcagtaggcc caacaattaa aacaagcgga ggttctgatc
     2761 gaaatcgatt ggtcgccatc gtcgttcccc aaaattacat acatgccaat tttcatcaca
     2821 ctcaatgcca tcttgctcaa ggagctgtcg ctgcaccact tggccaccat ggaaccccac
     2881 ggcaagccct ccctgagcat taaccaatcg atgccatggc agatcttcag ggccctggtg
     2941 cgcaatacca ccaacaattc tcgcagcacg aggtgatcca gccatcgcag cgagatcacc
     3001 atatgttgtt actttatcag caggaagatc tcgcatcagt tgatagacac gctcacgtat
     3061 gtttttctca tattcaagat tatccactta cagagccttt agatagtcag caagagcgtt
     3121 taaagcaact ttctcctcgc ccttttgggt acgcacactt acttcatgcg cttccatgtc
     3181 cttagggcca acaatcagtt gtacggggat tttcatagcc gtagcctcac gaatcttttt
     3241 accaaggctc tcattacgat catccgttgt gaatcgtact tcgttatatt ttacaggttt
     3301 catcacaact aaccccgata ataatgatgc aatttcatca acataaggct ttactgcatc
     3361 gttaatcgtc aagattcgta cttgctcagg tgccgcccag aatgggaacc agccacctgt
     3421 atgctcgata aagacactca agaatcgctc aatactacca agtaacgcac agtgaatcat
     3481 gatcggctgc tgctttgtcc catcttccgc tgcataaacc aagccaaaac gaccaggttg
     3541 tacaaagtca agctgtacag ttgccacctg gtgctcacgg ccaattgcgt cagtcgccat
     3601 aaagtcaatt tttggaccat agaatgcagc ttcgccctct tgctcaaagt attcaagctg
     3661 gtttttctca accgctgatt tcagctgcgc ctgtgctgac tgccatagat ctatgtcgcc
     3721 aaggtaacca tcaccatcat cacgatagct taaacgtaca cggagttgca taccaatcgt
     3781 ctggtaaagc tcgcgcgcgc tcgcaaggag cccatcaatc tcctgctcaa tctggtcagg
     3841 tcgggcaaac acatggctat catcctgtgt caatgaacga acacggttaa ggccgcctaa
     3901 ttcgccggtc ttctcatcac gataatcagt cgtagtttca aggaaacgaa ctggcatatc
     3961 acggtagcta cgaggctggc ttgcgtagat ttgcgtgtga tgagggcaat tcataggctt
     4021 cagtgccatt ttatcgtccg tttcttggct tgttaccaaa aatagctcat caccaaactt
     4081 agcccaatgc ccgctcgtct cgtaaagtgc tattttggta atatttgggg tccacacttt
     4141 agtaaagcca cgtgcttcac gaagctgatt agagaacgct gcgaccttct cacgaagcac
     4201 cgttccacga ggagtaaaaa gtggcagacc cgcaccaacg agcggagaca ttgtataaag
     4261 atcaagctct tttcctagac ggcgatgatc tcgggctttt gcttctttaa gcatcttgag
     4321 gtgctcatca agttcttctt tggttgcaaa tgccacacca tagaggcgct gcatctgggg
     4381 gttcttctca ttgccacgcc aatacgcacc tgcaacacgc attaacttaa aggcgcctac
     4441 cttacctgta ctttcagcat gtggaccacg gcaaagatcg gtaaagtcac catcagtata
     4501 gaatgatacc tgctcgacag cgacatcgcc atctgcagcc agccctagct cagctgcatc
     4561 caaatcctta gctattgtcg tacctgtgcg gcgaagatca ttcaaaagct cttctttata
     4621 tggctggcta tgctcttttg cccagtcaat tgcctcgtca acattcttct cacttcgttc
     4681 aaatgggaaa ttgcgcgcaa taataccacg catttctttt tcaatcttct taaaatcatc
     4741 ctcagaaatc ttggtgtccc caagatcaat atcgtagtaa aagccattct ctactactgg
     4801 acctacacca aatttagcct gtggccataa gtgctgcaca gcctgcgcag ttatatgagc
     4861 caagctgtga cgcattgcat aaagttgctc ttcgttcata tagaacctcc ttttaaatac
     4921 aaaaagcacg catatcgtat actttacgtg cgtgcctcgg tcctgaagct caggaggttc
     4981 caccttggct attctcttac tccggttctt ttacatggta ccgcaatcgc ttctcatggg
     5041 ctcaagagat ggttaggctc tatcatcgct ctagtagtag cataccgtaa atacactgtc
     5101 gttgcaatac cacccctgtt ctggtatgat atctattgtt ccaggtaacg tcgccgatgc
     5161 aaagcatcag tagctgaata atacttgggc gattagctca tttggctaga gcgccacatt
     5221 gacgttgtgg aggtgatagg ttcgaatcct atatcgccca ccaaacaaag accgacccca
     5281 tcaaggggtc gtttttgttt cttacatttc ccacatccaa tccacagttt tccacatcac
     5341 tttgggatca tttctatatc acgcaatccg cctggtgata tatcacccgg gccgccctat
     5401 gattgacatt tattactgct tatgctagaa taaaactatg agtaaagata caccccatgc
     5461 atcagagata aaagctcata atccaagttc tgcactcgac cgtttaaccc ttacatttga
     5521 atataacatc tcgtgggcag ctgaaaagaa gtccattaaa gaacgaactt cctaatccgg
     5581 gtttaagcta ataaagagaa gagcgacctt aggcgtcgct cttcttctgt ttcatcagtt
     5641 ccttatgccg ctcacgcgaa gccattgtcg ccttatccgt aacgtcgaac ttatcaagat
     5701 acttaccgag catcagatga gtctgcgcat ttagtgacgc gatagcgcta tagcagatag
     5761 atgttactgg cataagtatc cactgtaata ccat
//
LOCUS       DATIIX010000004         6323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_389434, whole genome shotgun sequence.
ACCESSION   DATIIX010000004 DATIIX010000000
VERSION     DATIIX010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6323
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389434"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..532)
                     /locus_tag="VLF64_00085"
     CDS             complement(77..532)
                     /locus_tag="VLF64_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90378.1"
                     /translation="MPSRNTKFYSLILMAADFIVLALVFGIAYYARTQLDHRVLLHTV
                     YAKEYLASFLAVAPIWILIFASLGLYSSQIYTRRLVEWSRVALGSLVGVLLIIGWEYT
                     TGGHFFPARLVTFYVLIGSALSIIIVREVLRFVQHQLFRYSRGVSLLIA"
     gene            587..1912
                     /locus_tag="VLF64_00090"
     CDS             587..1912
                     /locus_tag="VLF64_00090"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HSW90379.1"
                     /translation="MKHVRLQHIIAWLLLTIMGLIVIHAPLTVFLGTRFPQIAEGIKA
                     WKEVLMLIAFVLLTIDYIRRKALSAAFNDRLLWLIRGYVILHLVLLLVYRLPVNAVIA
                     GLMIDLRYLVYFLLVYLFLKTYPSYKESFLRVAMTGAVIVVGFAVLQLALPHNFLAYF
                     GYGDTTIQPYITIDRNPAFVRENSTLRGPNPLGAYAIMVLSGVAAYWVMQRKRLNDNT
                     RTTLIFFAIAGLISLIVSYARSAWLGAVIALVAVFAIKNKGIVTWRKAGYVAVATVIF
                     GALVYGARSSSLVQNTLIHNNPTTGSLVDSNTQHLESVTSGLASMFLHPLGQGVGSTG
                     SASLFTDNPTVVENQFLFVAHEAGWLGLALFVAITWMVLARLWHKRSDWMALAAFSSG
                     IGLIVVGLLLPVWADDTVSIVWWGLAAVILADGKGTNGKATNKKTKRAA"
     gene            1872..2261
                     /locus_tag="VLF64_00095"
     CDS             1872..2261
                     /locus_tag="VLF64_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90380.1"
                     /translation="MEKRPTKKQKELLNFIDGFIKGNGYGPSYREIMRALDYKSVSTV
                     ATHVDGLIQRGWLHKRDNEARTLEVVAPGDEPNPAIAAGIVSEGEKEIHAKLRELQKH
                     PDENANDTAVLLRTLVILGYEPKTEIA"
     gene            2285..4000
                     /locus_tag="VLF64_00100"
     CDS             2285..4000
                     /locus_tag="VLF64_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HSW90381.1"
                     /translation="MRQFVAEVPHLVIAKIQHVGWRKLLGGTIAVFFGVMILVQLFYP
                     ANTLLPYTKVDGISVAGKTKQQAIAILNKAYADHTIAIYMGSGREPVTSPKLSDVDGE
                     VDNTTRINNMDYPWYMRLVPTSLFWSSQKMATTPAPNFGDKFTLYISKKLMPNCQQQP
                     VNATLRAGNGKLSLVPAVTGGSCAEADVITSIKAIRPTLTAKTAVKVALSEHKPDVLD
                     SAATELADTLNLRLKAGTILLVNSNQVTITANDLLAWLDFTADSNKLIAKVNIDRASD
                     WLNKNVAAKVAVAAGISYVKTLDFTELSRIQGAPGRAIDIAATVSSLQQVVDGANTAA
                     AVATKTVPPSEQYTRTYSPDDAGLSALMTNYAHDHSGMFGISMVELDGQRRRASFNGE
                     NQFVTASTYKLFVAYELLKQIDAGKRDWDSNAACFNKMISQSDNACAESFLNMLGLST
                     ITNDIQAIGLKNSTFMKNGGPYTTANDLTLMLGMIATGQNFSSTNQQRLVAAMEGNVY
                     RQGIPAGVQGSVADKVGFLNGLFHDAAIVYGPHGTYVLAIMTDGSSWATVADLAKQID
                     NLHAQ"
     gene            4076..4150
                     /locus_tag="VLF64_00105"
     tRNA            4076..4150
                     /locus_tag="VLF64_00105"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4108..4110,aa:Asn,seq:gtt)
     gene            4367..>6323
                     /locus_tag="VLF64_00110"
     CDS             4367..>6323
                     /locus_tag="VLF64_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90382.1"
                     /translation="MARSVLGVISKIKKTKARASRALILFLVLTLTFGLVQPGASAAL
                     AESANARKFNTNFKLDPLKSKDAQPTTNLTQQTGGPLTLPTIKDPKSHKYEDTSKRTA
                     FTSTYVNNDGTKTLEYTPRQQNFNDGRAWQKIDNKLSDNSKPAPTPTLWESITNSAPK
                     AVAPTEFTGKSGIIDAQMKSLANGISFVVNNRKITMTPVGAKDVMPAKKDDTTVIYKD
                     AYPNVDIEYQLRGEAVKEIIVLKSNHAKTDLDFIVAGGKVINHPTKQGELTIEGMPPE
                     FSFSSLTLDVNGRGVISEQRVTQSATAKGIQVAVDKEWLKSQPASAFPMYIDPSFARD
                     STSYWMFKSDGYSCGASNCYANIGAIYDTSWKNWRTYVQFPYGDLAGKKVLNANMHGF
                     FQYGKNGITDTHWIAMGHANCIGYWCQGNQVASNPFSTDFDLNFTGELQNCVNNGDFG
                     AVWSFSGEEGAYKSFKPYYNLQASITYDTPTPVATPVTPADKQVMVDTMPTLKVNPVS
                     DADGDTVKYYFRVSTNSDAETGALINSGWIDSPQWTVPDGILQDGTTYYWHVYTLGAT
                     QTNPNWVNSFKVNLRTGKDSTQSYDTIGPVGVDLATGNASLSTGTHNIKALGGNIGLN
                     LNYNTPNKAKKGLKGEYWNVATNYNFAN"
BASE COUNT         1840 a         1421 c         1520 g         1542 t
ORIGIN      
        1 cggccaagcc accggggcca accccgagca atttagacaa atgttggaca ctccggccga
       61 catagcaggc aagcaactaa gcaatcaaaa gactcactcc gcgactatag cggaacagtt
      121 ggtgctgtac gaagcgaagg acctcacgca caataataat actaagtgcc gatccgataa
      181 gaacataaaa ggtgaccagg cgtgctggga agaaatgacc acccgtagtg tattcccacc
      241 caataataag gaggacgcca actaggctac caagcgcaac gcggctccac tctaccaagc
      301 ggcgtgtgta aatctggctt gagtaaagcc caagcgatgc aaaaataaga atccagatgg
      361 gtgcaacggc gaggaagctt gcaaggtatt ctttagcgta cactgtatgc aaaagcacac
      421 ggtggtcgag ttgtgtgcga gcatagtagg cgatgccaaa cacaagtgcg agcacgataa
      481 aatcggctgc catgagtatg agactgtaga attttgtatt tctcgatggc atattgtagg
      541 gattagtata ccactgtcag taggttttcg gtacaatcag ggcagcatga agcacgtacg
      601 gttacaacat ataatagcct ggctcctcct tactattatg ggattgatag ttatccatgc
      661 gccgctaacg gtgtttcttg gtaccagatt tccacaaatc gccgagggta taaaggcctg
      721 gaaagaagtg ctgatgctta tagcatttgt gttgctgact atcgactaca tacgtcgcaa
      781 agcgctatct gcagcattta atgacagatt actctggcta atcaggggct atgttatatt
      841 acaccttgta cttctactag tgtaccgttt gcctgtaaac gcggtaatcg ccggattaat
      901 gattgatctt cgctacctcg tgtacttcct gttggtatac ctattcctga agacctatcc
      961 gtcctataaa gaatcgttcc tgcgagtcgc aatgacgggt gctgtgattg tggtcggatt
     1021 tgcagtacta caactggcgt tgccacataa cttcttggcg tactttggct atggtgatac
     1081 aacaatccag ccgtatatta ccatcgatag aaatccagca ttcgtacgcg aaaacagtac
     1141 gcttcgtggg cctaatccac tcggtgctta tgcaattatg gtattgagtg gtgtggctgc
     1201 ctactgggtg atgcagcgaa aaaggctaaa cgataatacg cgcacgacac tcatattttt
     1261 tgccattgca gggcttattt cgttaattgt gagttatgcc cgaagtgcat ggcttggtgc
     1321 tgttattgca ctggttgcgg tatttgccat taaaaataag ggcatcgtta catggcgaaa
     1381 ggctgggtat gttgctgttg caacagtaat atttggcgcg cttgtttacg gtgcgcgcag
     1441 ttctagccta gtgcaaaata cgctcattca caataatcca acaacaggct ctttggtgga
     1501 ctctaacacc cagcaccttg aatcagtcac gagcggccta gcctcaatgt ttttgcatcc
     1561 acttgggcaa ggggtgggca gtactgggtc ggcgtcgtta ttcacagata atccaaccgt
     1621 cgtggaaaat caattcctgt ttgtcgcaca cgaggccggc tggcttggcc ttgcgttatt
     1681 cgttgcgatt acctggatgg ttcttgcgcg actatggcat aagcgatccg actggatggc
     1741 gctcgctgca tttagcagcg gcattggctt gattgttgta ggtttactgc tacccgtatg
     1801 ggctgatgac acagtgtcga ttgtatggtg ggggcttgcc gcagtgatct tggcggacgg
     1861 aaaggggaca aatggaaaag cgaccaacaa aaaaacaaaa agagctgctt aactttattg
     1921 atggatttat taaaggcaac ggctatgggc cgagctaccg cgagattatg cgtgcgcttg
     1981 attacaaaag tgtatcgacc gttgcaacac atgttgatgg tctcattcag cgcggctggc
     2041 tacataagcg cgacaacgaa gcacgcacac tagaggtagt agctccaggt gatgagccga
     2101 acccagctat agcggctgga atcgtatccg agggagagaa agagatccat gcgaaactca
     2161 gagaattaca aaagcaccct gacgaaaacg ctaatgatac tgcagtgctt ttgcgtacac
     2221 tcgtgattct agggtacgag ccaaaaaccg aaatagcata gaggtaatgg tatactagag
     2281 acatatgcga cagtttgtag cagaggtgcc acatcttgtt atcgcgaaga tacaacatgt
     2341 gggctggagg aaattgctcg gaggtacgat cgcggttttc tttggagtaa tgatactcgt
     2401 gcaacttttc tacccggcaa atacattgtt gccatacact aaggttgatg gcatatcggt
     2461 tgcaggcaag actaagcagc aagctatcgc tatactcaat aaggcgtatg ccgatcatac
     2521 gattgcaatt tatatgggat ctggcagaga gccagtaact tcgccaaaac tgtctgatgt
     2581 cgacggcgaa gtggataata ctacgcgtat taacaatatg gactatccat ggtacatgcg
     2641 acttgttccg acctcactct tttggtcgag tcaaaagatg gcaaccacac ctgcacctaa
     2701 ctttggtgat aaattcactc tatatataag caagaagctg atgcccaact gccagcaaca
     2761 accagtgaat gccacactta gagccggtaa tggtaaattg tcacttgtcc cagcagtgac
     2821 aggcgggtca tgtgcagagg cggatgttat tacaagtatt aaggccattc ggccaacact
     2881 taccgctaag acggcagtaa aagttgcgct tagcgagcac aagccggatg tcttagatag
     2941 tgccgccacc gagcttgcgg atacactaaa ccttcgactg aaagcaggca caatattgct
     3001 tgttaatagt aatcaggtca ctattacagc aaatgactta cttgcttggc tcgattttac
     3061 ggcagacagt aataaattaa tcgctaaagt gaatattgat cgcgctagcg attggctgaa
     3121 taaaaatgta gctgcaaagg tggctgttgc tgcgggcatc agctacgtaa aaacactcga
     3181 ctttaccgag ctatcgcgca tacagggtgc ccctggtagg gcaattgata tagccgcgac
     3241 ggtaagctca ttgcagcaag ttgtagatgg cgcaaacacc gcagcagccg ttgcaacaaa
     3301 gactgtccca ccaagcgagc aatacactcg aacgtacagc cctgacgatg ctggcctttc
     3361 tgcgctaatg acaaattatg cgcatgatca ctcaggcatg tttggcatat ctatggtaga
     3421 acttgacggg caacgccgcc gtgccagctt taacggcgag aaccaattcg taacagcgag
     3481 tacatacaag ctatttgtag cctacgaatt actcaagcaa attgacgccg gcaaacgtga
     3541 ttgggatagc aacgcggcat gctttaataa aatgattagc caaagtgaca atgcttgcgc
     3601 cgaaagtttt ttaaacatgt tgggtctttc gacaatcact aatgatatcc aggcgatcgg
     3661 acttaagaac tcgacattca tgaaaaatgg tggtccatat acaactgcaa atgatcttac
     3721 gctaatgctt ggtatgattg caacaggcca aaatttctcg agtactaacc agcaacggct
     3781 cgttgcggca atggagggca acgtatatcg ccagggcatc ccagcgggcg tgcaaggctc
     3841 ggttgccgat aaagtaggat ttctaaatgg gctattccac gatgcggcaa ttgtatacgg
     3901 gccgcacggt acatatgtac tcgcaattat gaccgatggc agcagctggg ccacagttgc
     3961 cgaccttgca aaacaaattg ataatttaca cgctcagtaa tatttacaga tacagtccaa
     4021 tgctgtacaa tagcccttgt attcatcccc ggggtgacgc accttacatt actattccgg
     4081 catagctcag tggtagagcg gatcgctgtt aacgattagg tcgctggttc gagcccagct
     4141 gccggagcca agaatttatt tcattacaat aagccatcat tccgagtagc ttttttgtat
     4201 ttccgcctca gtaaagcgga cttatccaat aagattccat cttttatggt ataactgtta
     4261 acttaaagta aataattagc gtattcttaa attctagtaa aaatcctatt gctaatatat
     4321 tttgcgtcgt atatgctgaa agcatcaaag ttgtggaggg ggttctatgg cgcgaagtgt
     4381 gttaggtgta attagtaaaa tcaaaaaaac gaaagcacgt gctagtcgcg cgcttatttt
     4441 gttcttagtg ctcacattaa cctttggtct tgtgcaacca ggggcttcag cagcattagc
     4501 cgaatcggca aatgcacgaa agttcaatac gaatttcaaa ctcgatccac taaagtcaaa
     4561 agacgctcag cctacaacga acttaacaca acaaacaggc ggcccattaa cgttgccgac
     4621 catcaaagat ccgaaaagtc ataaatatga ggatacgagt aagcgtacgg catttacttc
     4681 gacctatgtg aataatgacg gtacaaaaac actcgagtat acaccacgcc aacagaactt
     4741 taatgatggc agggcgtggc aaaagattga caataaactg tcagataaca gcaagcctgc
     4801 accaactcca actctctggg agtctattac aaacagcgct ccgaaagccg tcgcgcctac
     4861 tgagtttacc ggaaaatcag gcatcataga tgctcagatg aaatcgcttg caaatggtat
     4921 tagctttgtc gtaaataaca ggaaaattac catgacacct gtaggggcaa aggacgtcat
     4981 gccagcgaaa aaggatgata caactgttat ctataaagat gcctacccaa atgttgatat
     5041 cgagtatcaa ctgcgaggtg aggctgttaa agaaatcatt gtcctcaaaa gtaaccacgc
     5101 aaaaactgat cttgatttta tagtcgccgg tggtaaggtc atcaatcacc caaccaaaca
     5161 gggtgagctg acgatcgaag gcatgccgcc agaatttagc ttcagctcgc taacacttga
     5221 cgtaaacgga aggggcgtca ttagcgagca gcgcgtaaca caatcagcca cggcaaaagg
     5281 gatacaagtt gcggttgata aagagtggct caagagccag cccgcttcgg catttccaat
     5341 gtacatcgac ccaagctttg cgcgtgatag tacgagctat tggatgttta agtctgatgg
     5401 ctacagctgt ggcgcaagca actgctacgc aaacatcggt gcaatctacg atacaagttg
     5461 gaaaaactgg cgtacatacg tacagttccc atatggtgat ctagcaggca aaaaagttct
     5521 taacgcgaat atgcatggtt tctttcagta cggtaaaaat ggcataaccg atactcattg
     5581 gattgcgatg ggacatgcta actgcattgg ctattggtgc caaggaaacc aggttgcctc
     5641 aaatcctttt agcacagact ttgacttgaa ttttacaggt gagctgcaaa actgcgttaa
     5701 caatggtgac tttggcgcgg tgtggagctt ctcgggcgaa gaaggcgcat acaagagctt
     5761 caaaccgtat tacaatttgc aggcaagtat cacttatgac acgccaacac ctgtcgcaac
     5821 cccagtaacc ccggcagata agcaggttat ggttgacacc atgccaaccc tgaaagttaa
     5881 tcccgtaagc gacgcagatg gggatacggt aaagtactac ttccgcgtaa gtacaaacag
     5941 cgacgcagaa accggtgcgc tcattaactc gggttggatt gactcaccac agtggacagt
     6001 cccagatgga atcttgcaag atggtacgac gtattattgg cacgtctata cactaggagc
     6061 aacgcaaaca aaccccaact gggtaaactc atttaaagtt aatttacgca caggtaaaga
     6121 cagtacccaa agttacgata cgatcggccc agtcggtgta gatctagcta cgggcaatgc
     6181 ctcgctctcg acgggcacac acaatatcaa agcactgggt ggcaacattg ggttgaacct
     6241 taactacaat accccaaata aggcaaaaaa gggattgaag ggtgaatact ggaacgttgc
     6301 tacaaactat aatttcgcaa atg
//
LOCUS       DATIIX010000005         4447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_195341, whole genome shotgun sequence.
ACCESSION   DATIIX010000005 DATIIX010000000
VERSION     DATIIX010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195341"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VLF64_00115"
     CDS             <1..646
                     /locus_tag="VLF64_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4082 domain-containing protein"
                     /protein_id="HSW90383.1"
                     /translation="TTLLGTTANLAAPALVGPGTVQSPSAYGLPATVPVDYKTIYTST
                     LVPTSTIANDSTDYTLGTLFTSSSDGKIYGARWYFPDILPTHPVIATLYSWTSDTTGT
                     ALASATFSNPQTGWSQVLFTTPISITANTKYVICVWTIDYYASTSGLFASSSVTDGDL
                     TAPQTTSSVHNGKYQAGTGSSTYPTQSFNGGGYFADVLYIGDGTVQFEGWGIPT"
     gene            complement(814..2022)
                     /locus_tag="VLF64_00120"
     CDS             complement(814..2022)
                     /locus_tag="VLF64_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90384.1"
                     /translation="MSEVIEMARSAEVFDDTTAGQLNERLADKTVEQLGATKFELAKQ
                     LGTMPNAFKVAGTLLDFMREAELIGNTTPAAPAQPMAVTVVMPTKRSDMGLGELFNEL
                     TEHPEDADELVALIGQQNSVQQANAKLHGSGEWAIPNEDGLLNVAETLEYVRHLAAKY
                     TQPQRKWKSQYWPTTLERALGRDQRIMLYPFATGAQNLLLVGPDRFGNDWSKLSDNVH
                     EAVIHAVTRHILAVNNETDTRRVTHELFSDELPAYLRDIVEDYEQGKARGVKVERYAT
                     PEQLQAAGLDVDSFGSRHPFGAEPEHDDAWYEARLREVALAPINKPNGDIRKSRCVLT
                     SVSAAHGDVRLDNVIVLGFVQLAHGDLEGTFYMPSRRSPSLAHGDDDSIVRDLPWKRL
                     YAKAQELGLI"
     gene            complement(2199..2366)
                     /locus_tag="VLF64_00125"
     CDS             complement(2199..2366)
                     /locus_tag="VLF64_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90385.1"
                     /translation="MNFYNLRRPSVPLYNANPSTKEDIQELIRVTKRSSITAWLAIAI
                     SAASFLVATFK"
     gene            complement(2452..2658)
                     /locus_tag="VLF64_00130"
     CDS             complement(2452..2658)
                     /locus_tag="VLF64_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90386.1"
                     /translation="MVKKPANSGQHWTPSQISQLKSLAKHNTPTRVMGLKLERTPGAV
                     QSKAQDLGVSLKPTNQSPYGTKKK"
     gene            3233..4000
                     /locus_tag="VLF64_00135"
     CDS             3233..4000
                     /locus_tag="VLF64_00135"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HSW90387.1"
                     /translation="MDFPDDDVCLDLIFKARFGHVKDCPRCGVIDTKFFRVAGRKCYA
                     CMHCSYLFSASKNGVAAKELERHLGVTYKTAHRMGHQVCRLMFLAEKIGGRGITVEAD
                     ETYYGGRRRFSAGPRHKVPVLSVVERDGEVRAQVSDTASMRRAKTFLHENIEFDSTLY
                     SDESKIYLWTKKYGIKHDSVNHARREYARHRVSTNTIEGFWSQLKRSIDGTYHRVSRK
                     YLQLYVDEFTWRYSHRSQAMYPILLGRAALCTIWDGG"
     gene            complement(3997..>4447)
                     /locus_tag="VLF64_00140"
     CDS             complement(3997..>4447)
                     /locus_tag="VLF64_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90388.1"
                     /translation="AISGNKTQAAKELARVSEGLGISAADQVIEIFKRYESRPFQVDT
                     NKSFRGRVMKEGKDIKVVTGDITLGVTGRWPSQWLADGKKYNLPSKQRTTGREVMRTI
                     SPADSVGITFSESHYLKDSSNMNEVIETGKQGKNFYTRLKDVGLLDD"
BASE COUNT          932 a         1184 c         1197 g         1134 t
ORIGIN      
        1 gacaacatta ctaggtacga cggccaatct tgcagctcct gctctcgtag gaccggggac
       61 agtacaatca ccttcggcat acggtcttcc cgccaccgtt cctgtcgact ataaaactat
      121 ttatacatcg acacttgttc caacttctac gattgccaat gatagtactg actataccct
      181 tggtacgctc ttcacgtcat cgagcgacgg taagatctat ggtgcacggt ggtatttccc
      241 tgatattctt cctacacatc ctgttattgc aacgttatat agctggactt cagatacaac
      301 aggcacggct ctcgcttcag ctacttttag caatcctcaa acaggttgga gccaggttct
      361 ctttacgacg cccatctcga ttactgctaa tacgaaatac gttatttgtg tatggacgat
      421 tgattactat gcatcaacct caggattatt tgcatcaagt agtgttaccg acggtgacct
      481 aacggcgccg cagactacct cgagtgtgca taacggtaaa taccaagcgg gtacgggtag
      541 ttcgacctac cctacccaga gctttaacgg tggaggttat ttcgcggacg tgctgtatat
      601 aggtgatggc acagttcagt ttgaaggttg gggtattccg acttaggttt gagttgcgga
      661 tattgcccgc agttggcttt atttttctga ataaacaaaa aaccagcgtt tgctggtgta
      721 agagatgttg aataaagtga tattaaacta aggaaggtgt ggggacggcc gaagccgccc
      781 cctcccttct catcttgctg acgatcgaac tgctcagatc aggccgagct cctgggcctt
      841 ggcatagagc cgcttccagg ggaggtcacg aacgatggag tcgtcgtcac cgtgagccag
      901 actggggctg cgcctcgaag gcatgtagaa cgtgccttcg aggtcaccgt gggccagctg
      961 gacgaagccc aggacgatca cgttgtccag tcgtacatca ccgtgtgcgg cgctgactga
     1021 cgtcaggacg cagcgactct tgcggatgtc tccgttcggc ttgttgatcg gagccagagc
     1081 gacctcacga agacgcgcct cgtaccacgc gtcatcgtgc tcgggctcag cgccgaaagg
     1141 gtggcggctc ccgaaggagt cgacatccag accggcagcc tgaagctgtt ccggcgtggc
     1201 gtagcgctcg accttgacgc cgcgcgcctt gccctgctcg tagtcctcga cgatgtcccg
     1261 caggtacgcg ggcagctcgt cgctgaacag ctcgtgcgtg acgcggcggg tgtcggtctc
     1321 gttgttgacg gcgaggatgt gccgcgtgac cgcgtggatc accgcctcgt gaacgttatc
     1381 cgacagcttg gaccagtcgt tgccgaaccg gtccggtccg acgaggagca ggttctgggc
     1441 gccggtcgcg aacggataca gcatgatgcg ctggtcccgt ccgagcgctc gctcgagcgt
     1501 ggtcggccag tactggctct tccacttgcg ctggggctgg gtgtacttgg ccgccaggtg
     1561 gcggacgtac tccagcgtct cggcgacgtt gagcaggccg tcctcgttgg ggatggccca
     1621 ctcgccgctg ccgtggagct tggcgttcgc ctgctggact gagttctgct ggccgatgag
     1681 ggccaccagt tcgtcggcgt cttcggggtg ctcggtcagc tcgttaaaga gctcaccgag
     1741 tcccatgtcg ctacgcttgg tcggcatgac cacggtgacc gccatcggct gggccggagc
     1801 ggccggggtc gtgttcccga tcagttcggc ctcgcgcatg aagtcgagca gggtgccggc
     1861 caccttgaag gcgttgggca tcgtgccgag ctgcttggcg agttcgaact tggtggcgcc
     1921 gagctgttcg acagtcttgt cggcgagtcg ctcgttgagc tgtcccgccg tggtgtcgtc
     1981 gaagacctcc gcgctacggg ccatctcgat gacctcgctc atgctggtgt tgctcacttt
     2041 cggtctcctt gaagttcgat gtgcagatga aaagggcgat gtttccgccc ttttggagcg
     2101 taaaatcttt catattttaa cataaaaatc aataatcttc aatagtcatc tcgggcgact
     2161 tctcgggcga ctatcccctc acaacaagta cgctagattt atttaaatgt ggcgacaaga
     2221 aaactagccg cactgatagc gatggccagc catgcggtaa tgcttgagcg tttggtcacg
     2281 cggataagtt cttgaatgtc ttctttcgta gaaggattgg cattataaag tggcaccgat
     2341 ggtctccgta ggttataaaa attcattcag ctacaatagt agtatgtgat atgctgtacg
     2401 ttgtgactca agctcggctc ttgagtcccg gaccacgatt aacgtcgtgg tctacttctt
     2461 tttagtgccg taagggctct ggttcgtcgg ttttaagctg acgccaaggt cttgtgcttt
     2521 gctctgaacc gcacctggtg tgcgttcaag cttcagaccc atgacccgcg ttggcgtgtt
     2581 gtgcttcgca agagatttca attgagaaat ctgcgaaggt gtccaatgct ggccggaatt
     2641 ggctggcttc tttaccataa aattcctttc ctggagcact gacgaatggg gcattggctc
     2701 ctgaaatcgc aatcgtcggc cgagacgacg aggtgatctc gtttatccgc cccctagttg
     2761 tgacaagagt tgcacgcaac aaggcgacag acaaaaagga tccttaacct ctcgcccgtg
     2821 ctccctacct acgtaccttt cacttttaat tttatcaccc aattgtgtca ttggaaagca
     2881 attagacgct atatatgccg tgtttattgc tacatcacta cttttgcagc ctggcaattc
     2941 ggttatgctc gtaatcaaaa aggggttatg ataaagtaac gaacaacgcg acttcgcacg
     3001 tcgtaataga gagtctttct tcccaccaca aacaaacgcc agggggcgtc tgcaccttca
     3061 tcactatttt gctagcacct cctttgggtg ttttattttg gttacgctct cgcgttgaca
     3121 tcaacctaaa gaaaggaggt acatactgaa cgcccttcca ttttcttttt actcaaccta
     3181 tttttctctg ctcgtgaggc gcagcaggag atataccgtc aagcagttcc ggatggactt
     3241 tccagatgat gacgtctgcc tcgaccttat tttcaaggcg cgctttggcc acgtgaagga
     3301 ttgcccacgc tgcggcgtca tagacacgaa gttttttagg gttgctggac gtaagtgcta
     3361 cgcctgcatg cactgcagct acctgttctc agcctctaag aacggcgttg cggctaagga
     3421 gttagagcgc catcttggcg ttacatacaa gacagcccat cgcatgggcc accaggtgtg
     3481 ccgactgatg ttccttgcag agaagatcgg tggccgcggt ataaccgtcg aagccgacga
     3541 aacatactac ggtggtcgac gcaggttctc ggccggcccg cgacacaagg tccccgttct
     3601 cagtgtggtg gagcgtgacg gggaggtgcg agcgcaggta tccgacacag cctcgatgag
     3661 gcgcgcgaag acatttcttc acgagaatat cgagtttgac agcaccctct atagcgatga
     3721 gtcgaagatc tacctctgga cgaaaaagta tgggatcaag cacgactcag ttaatcacgc
     3781 gcgccgcgag tatgctcgcc atcgcgtgtc gaccaataca attgaggggt tctggagcca
     3841 gctaaaacgc tcaatcgacg gtacgtatca tcgtgtctca cgcaagtatc tgcagctcta
     3901 tgttgatgag ttcacctggc gctattcgca tcgcagtcag gctatgtatc caattttgtt
     3961 aggacgcgcg gcattgtgca ctatttggga cggcggctag tcgtctagta aaccaacgtc
     4021 tttcagccgt gtatagaagt tcttaccttg tttgccagtc tctattacct cgttcatatt
     4081 acttgagtct ttaagatagt gactttcgct gaaggtgatg cccacactgt cagcaggaga
     4141 tatggtccgc atgacctcgc gaccagtggt gcgttgctta cttggtaggt tgtacttctt
     4201 cccgtctgcc agccactgac ttggccatcg tccagttacg ccaagtgtaa tatcgccagt
     4261 gacgacttta atgtccttgc cctctttcat tacccgaccc cggaaggact tattcgtatc
     4321 aacctggaag gggcgtgatt catatcgctt aaatatttca atcacctgat cggctgccga
     4381 tatgcccaag ccctcactga ccctcgccag ctctttggca gcctgtgtct tattaccgga
     4441 gatagcc
//
LOCUS       DATIIX010000006         4715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_473244, whole genome shotgun sequence.
ACCESSION   DATIIX010000006 DATIIX010000000
VERSION     DATIIX010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473244"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VLF64_00145"
     CDS             <1..743
                     /locus_tag="VLF64_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HSW90389.1"
                     /translation="ILDNLDKEGVDTSHVVQNPEYQSGYSVILIATNGERTIMNHRGR
                     AFGRTGRRAFDLSSIKEYDWVYPTSLGDGGLPLLREIVEEAEKHGAKVMLNPAGPELA
                     DKDRLIALLDSVDVLCVNKEEMQMLVSGETLEELALHALHYVPVAIVSDGPNGVVATD
                     GKTIVRAGMYEDVKVVDRTGAGDAFASGFLSQWSQGKSLRESIIFASANSTSVVTKIG
                     AKDGILHKGVKLHDMPIHEKPVNMRESK"
     gene            743..2245
                     /locus_tag="VLF64_00150"
     CDS             743..2245
                     /locus_tag="VLF64_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HSW90390.1"
                     /translation="MAKTLSMLLDAKEPMFSIALRELEIMTGSKGIDVAYIADILHRA
                     HALMRVIGLDPSDTTEQELYKALEAHINNESLFHNMVDVGLVFEGRHVISFNPEDIRT
                     NATVPYEQRTFVNLQRAVTGALLERYEIASGDIDRVAAAAKQAGMVKQTVEEPTDNRP
                     KKIEVKEGELMPNILCVGDIFTDAFIQLPEGSAKVIKEEDGKEWLALPFGQKPPYDHV
                     DIVRSVGPSPNSAVSMSRLGINTSLMAWIGGDDTGKEALAHLVEEHINTDSMVTEPEN
                     KTSYWYVLRYGADRTMLVRSEKYKYEWRDPSVRPDWIYLSYIGELAWDLHEKLLDYLQ
                     RNPDVKLAFQPGTFHFQWGVEKLAGIYGRSEIVVMNREEAVDVTGASYDNLHELANAL
                     HAMGPKYVVITDGPKGSYASHDGKLVTIPNYPDPAPPLDRTGAGDAFASTIVAGLALG
                     EDFEIALTWAPINSMSVVQQLGAQAGLLKLPKLEEFLANAPADYEVKELS"
     gene            2245..2865
                     /locus_tag="VLF64_00155"
     CDS             2245..2865
                     /locus_tag="VLF64_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90391.1"
                     /translation="MRYQICVSGAAAGETVVGAHDLAFQLGEEIAKAGKTLLTGATVG
                     LPHYAAMGFMSVADSRGSSVGFSPASSYREHVATYRLPTKEFDYINFTGMEYGGRDVH
                     LVRSADAVITVGGRMGSLHELSTALESRKVCGVLLGSGGLADYAATLLQHIEAPGGRD
                     VIYDTDPARLVARVIELLDKKYADFHDVIDAPLTDAVQSDLKKRRG"
     gene            2894..>4715
                     /gene="uvrB"
                     /locus_tag="VLF64_00160"
     CDS             2894..>4715
                     /gene="uvrB"
                     /locus_tag="VLF64_00160"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HSW90392.1"
                     /translation="MNKFHLTSKYQPTGDQPTAIAQLIDGLEKGEREQTLLGVTGSGK
                     TFTMANIIANRNVPTLVLAHNKTLAAQLFSEFKSFFPDNEVHYFVSYFDYYQPEAYIA
                     SSDTYIEKDSAINEEIDRLRHAATSALLTRRDVIIVASVSCIYGIGSPDDYAEMAITV
                     KRGERRQQDKFIRLLTDIQYTRNDIDFSRGTFRVRGDVVDVCPAGSETAYRFEFFGDD
                     VDRITQIDPLTGEILSEPDQITIFPSSHYVTPKEKVARAVEKIKKEFDERMNYFESHD
                     KLLEAQRLSQRTKYDIEMLEETGFVKGIENYSRYLTNREPGEQPATLLDYFPDDFLLL
                     VDESHVSLPQVRGMYNGDRARKEVLVEYGFRLPSALDNRPLRFDEFERHVNQTIYVSA
                     TPSEYELSHSPEPAQQVIRPTGLLDPPIDVRPVDTQVDDLIAEIRDRIVKHQRVLATT
                     LTKRMAEDLSAHLIELGIKTAYIHSEVDTLERGDILRDLRMCVYDVLVGINLLREGLD
                     LPEVSLVAILDADKEGFLRSESALIQTIGRAARHVDGRVIMYADRITGSMQRAIDETN
                     RRRDIQHAYNVSHNITPRGVDKVIDEGLRAIIPQKEDTKHE"
BASE COUNT         1297 a         1084 c         1216 g         1118 t
ORIGIN      
        1 ccatcctaga caaccttgat aaagagggcg tcgacacatc acatgtcgta caaaatccag
       61 aatatcagtc cgggtattca gtaatcttga ttgcgacaaa cggtgaacga acaatcatga
      121 atcatcgcgg ccgtgcattc ggtcggacag gtcgccgcgc gtttgatttg tctagcatta
      181 aggagtatga ctgggtgtat ccaaccagcc ttggcgatgg cggtttgcca ttactccgcg
      241 agattgtaga agaagctgaa aaacatggtg caaaggtaat gttgaaccca gcggggcctg
      301 aactcgccga taaagaccgt ttaatcgcgc tgctcgattc tgtagatgta ctttgtgtga
      361 ataaagaaga aatgcaaatg ctcgtaagcg gtgaaacgct tgaggagctt gctctacatg
      421 cccttcacta tgtgccagtt gcaattgtga gcgatggccc gaatggtgtt gtggcgacgg
      481 atggaaaaac aatcgttcgc gctggcatgt atgaggatgt aaaggtagtt gaccgcacag
      541 gcgcaggcga tgcatttgca agtggcttct tgagtcaatg gtcacagggc aaatcgctcc
      601 gcgaatcaat tatttttgca agtgccaact ctacaagtgt agtaacgaag atcggcgcta
      661 aagacggcat cttacataaa ggtgtaaagc ttcatgatat gccaatccac gaaaagccgg
      721 tcaatatgcg ggagtctaag tagtggcgaa gacgctaagc atgctcctag acgctaaaga
      781 gcccatgttt agcatcgcgc tccgtgagct ggagataatg acgggtagta aagggattga
      841 tgtcgcatat attgcggata tccttcaccg tgcacacgcg ctgatgcgcg taatcggcct
      901 cgacccatct gatacgaccg aacaagagct ttataaggcg cttgaggcac atattaataa
      961 tgaatcattg tttcataata tggtcgacgt aggtctcgta ttcgagggga ggcatgtcat
     1021 ttcatttaac cccgaagata ttcggacaaa tgcgacggtc ccctatgagc aacgtacatt
     1081 cgtgaatctg cagcgtgcgg taaccggcgc tcttctagag cggtatgaaa tcgcctctgg
     1141 cgatatcgac cgagtagctg cggctgcaaa acaggctgga atggtaaaac agactgtcga
     1201 ggagccaact gataataggc cgaaaaagat agaagtaaaa gaaggagaat tgatgccgaa
     1261 tatactatgc gttggtgata ttttcacaga tgcgttcata cagctacccg aaggttcagc
     1321 gaaggtgatt aaggaagaag atggtaaaga atggctcgca ttgccatttg gccagaagcc
     1381 gccatacgat catgttgata tcgtacgctc cgttggcccg tccccgaact ctgccgtgtc
     1441 aatgtcgcgt cttgggatta atacaagtct aatggcctgg atcggcggcg atgataccgg
     1501 taaagaggca ctcgcccacc ttgttgaaga gcatattaac accgacagca tggtaactga
     1561 gcctgagaac aagacgagct actggtatgt gctgcggtac ggtgcggatc gtacaatgct
     1621 tgtacgcagc gagaaataca aatacgagtg gcgcgatcct agtgtaaggc ctgattggat
     1681 atatctttca tatataggtg agcttgcgtg ggacttacac gaaaaattgc tcgactactt
     1741 acagcgcaat ccagatgtaa agcttgcctt ccagcctgga accttccatt tccaatgggg
     1801 cgtcgagaaa cttgctggta tctacggacg cagtgagatc gttgtgatga atcgtgaaga
     1861 agcagttgat gtaacgggtg cgagttatga caatctacat gagcttgcga atgcattgca
     1921 tgctatgggt cctaaatatg tggtaattac cgatgggcca aaaggctcat acgcatcaca
     1981 cgatggcaag cttgttacaa tccccaacta ccccgaccct gcaccgccac ttgatcgtac
     2041 cggcgcaggc gatgcatttg caagcacgat tgtagccggc cttgcgcttg gtgaagattt
     2101 tgaaatagcc cttacatggg cgccaatcaa cagtatgagc gtcgtacagc aactgggcgc
     2161 acaggctgga ttattaaagc tccctaagct tgaggaattt ctcgcgaatg ccccagcaga
     2221 ttacgaagtg aaggagttaa gctaatgcga tatcagatct gtgtatccgg agcggcggca
     2281 ggcgaaacgg tagtaggtgc acatgacctg gcatttcagt taggtgaaga aatcgcgaag
     2341 gcgggcaaga cattactaac cggtgcaacc gtagggctgc cacactacgc agcaatgggc
     2401 tttatgagtg ttgcagacag cagaggaagt tccgtcggat ttagtccggc tagcagttat
     2461 cgagaacatg tggcaaccta tcgcctgcca acgaaagagt tcgactacat taacttcact
     2521 ggcatggagt acggcggccg tgatgtacac cttgttcgca gtgctgatgc agtgattact
     2581 gtcggcggtc gtatgggtag tctccacgag ctatcaactg cactcgaaag ccgcaaagtg
     2641 tgtggagtcc tccttggcag tggcggactc gctgattatg cagcgacact tctgcagcat
     2701 attgaagctc caggtggccg cgatgttatt tacgacaccg atccagcacg cctcgttgca
     2761 agagtgatcg aattactcga taagaaatat gctgactttc acgacgtcat tgatgcgccg
     2821 ctaactgacg ctgtccaatc tgatcttaaa aagcgtcgcg ggtagtctcg gcggaaccgt
     2881 gtataataaa gaggtgaata aatttcacct tacttctaag taccaaccga caggtgacca
     2941 gccaacagca atcgcccagc ttattgacgg cttagaaaag ggtgaacgcg agcaaacatt
     3001 gcttggtgta actggttcgg gcaagacctt tacgatggca aatattatcg ctaatcgtaa
     3061 tgtgccaaca ctcgtactag cgcataacaa aacacttgcc gctcagctat tcagcgagtt
     3121 taaatcattt ttccccgaca acgaagttca ctattttgtg agctatttcg attactacca
     3181 accagaagcg tatatagcga gcagtgacac ctatattgaa aaagactcag caattaacga
     3241 agagatcgat cgtcttcgtc acgcggctac tagtgcacta ctaacgcgtc gcgatgtaat
     3301 cattgttgcc agtgtgagct gtatctacgg tattggttcg ccggatgatt atgcagaaat
     3361 ggcaatcaca gtaaagcgcg gcgagcggcg ccaacaggat aaattcatac gcttgcttac
     3421 ggatatccag tacacacgca acgatattga ttttagtcgc ggtacatttc gtgtgcgcgg
     3481 tgatgtggtt gatgtatgtc cagccggcag cgaaacggca taccgattcg aattctttgg
     3541 ggatgacgtc gatcgtatta cgcagattga cccactgacg ggcgaaatct taagcgagcc
     3601 cgatcaaatc acaatcttcc cgtcaagcca ctacgtcaca ccaaaagaaa aggtggcacg
     3661 tgctgtcgaa aagatcaaaa aagaatttga tgagcgaatg aattattttg agtcacacga
     3721 taaattactc gaggcccaac gactcagcca gcgaaccaag tatgacatag aaatgctcga
     3781 agaaaccggc tttgtaaaag gtattgagaa ctacagccgg tatctaacta atcgcgagcc
     3841 aggtgagcaa ccggcaaccc tactcgatta cttcccagat gattttttat tacttgttga
     3901 tgaaagtcat gtcagtctac cgcaggtgcg gggtatgtac aacggcgacc gcgctcgtaa
     3961 agaagtgctc gtagagtacg gcttccgctt gccaagcgcg cttgataatc gcccattgcg
     4021 ttttgatgag tttgagcgcc atgtcaacca aacgatctac gtatcggcaa cgccaagcga
     4081 gtatgagcta tcgcatagcc cggaacctgc ccaacaagtg attcgcccaa caggactcct
     4141 cgatccgcca attgatgttc gtcctgtcga tacgcaggtg gatgatttaa ttgctgagat
     4201 acgtgatcgc atcgtaaagc accagcgtgt actcgctacg acactgacca aacgtatggc
     4261 cgaggacttg tctgcccatt taattgagct cggtatcaaa accgcatata ttcacagtga
     4321 agttgatacg ctagagcgtg gagatattct acgcgacctc cgtatgtgtg tatatgatgt
     4381 gctagtgggt attaatttac tgcgtgaagg gcttgatttg ccagaagtaa gcttggtggc
     4441 tattcttgat gcagataaag aaggcttcct tcgtagcgag agcgcgctca ttcagacgat
     4501 tggccgtgcg gcacgtcacg ttgacggtcg ggtaattatg tatgcggacc gaattactgg
     4561 cagcatgcaa cgagcaatag atgaaaccaa ccgccgccgc gacatccaac atgcctataa
     4621 tgtgtcacac aatattacgc cacgcggtgt cgacaaggta attgatgaag gcctccgcgc
     4681 gatcattccg caaaaagaag acacaaaaca cgaga
//
LOCUS       DATIIX010000007         3362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_445468, whole genome shotgun sequence.
ACCESSION   DATIIX010000007 DATIIX010000000
VERSION     DATIIX010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445468"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VLF64_00165"
     CDS             <1..226
                     /locus_tag="VLF64_00165"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HSW90393.1"
                     /translation="LDSLYNTRLYKGLPPGPIASPSEYALKAAANPASTDYLYFIAGD
                     EGGGAMYFSKTEAEHEQNIKDHCQVLCGTV"
     gene            537..1697
                     /locus_tag="VLF64_00170"
     CDS             537..1697
                     /locus_tag="VLF64_00170"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HSW90394.1"
                     /translation="MTVRRSSLRESSSAQQTAKSSWARGSISGTTIATYLGIFLLIIS
                     MIALAYHPPVSQKALADASNGASTQQNQTQTTQNVSVDQLVATDLASSLAESTDMPIS
                     NNVANLSQTLSVESVLAQSDSNVISKPQIIQPTANGRTLQYYIAGVGDTVPSVAAKYG
                     ISAQTIEWGNNLTSEALTPGQKLTILPTDGVLYTVKSDDTLDSIVAKYGADRSSIVAF
                     NDLELTGNPAVGTQLILPGAVLPNNERPGYVTPVTGGGGGNYSGGYGNAYLAKASVGN
                     RYAWGNCTWYAYERRLELGMPVGSFWGNASSWASYARQAGYLVDGNPTPGAVMQNGGG
                     YGHVAIVESVNPGVSVTISEMNGYRFGGGFARVGHGDIPWSEAVSGYYNYIH"
     gene            1835..3121
                     /gene="obgE"
                     /locus_tag="VLF64_00175"
     CDS             1835..3121
                     /gene="obgE"
                     /locus_tag="VLF64_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HSW90395.1"
                     /translation="MFVDTATVTVQAGKGGDGAVSFRHEIYVDKGGPDGGDGGKGGDV
                     IFLATEQLNTLLSFRYQPRLIANNGINGGGTRKAGRMGKPLIVKVPVGTLVRRKGEVV
                     ADLVKPGQEVVIAKGGDGGFGNAHFKSSVRQTPRIAELGEMGESFEAELELKLLADVG
                     LVGFPNAGKSTFLSVVTNARPEIADYAFTTLTPNLGVADIDDGHLLIADIPGLIEGAS
                     EGKGLGDKFLRHVERTAVLLHLIDAYSDDIAGSYMAIRRELETYSTQLINRPEIVALT
                     KCDGLDDDIIEMQKQSLRAVMPVNSEIYAISSSAHQGLTELLRGLRREVTAVRALAPD
                     NEELDETPVITLGAESLSDHWEVTFDEVNAIYHIHGEKIEKFARRTNYELYENVNRLR
                     DIMRRLGITHELARMGASGMSMVQVGDSTPFNLTEQ"
BASE COUNT          880 a          741 c          870 g          871 t
ORIGIN      
        1 cctcgattcg ttgtacaata cacgtctcta taaagggctt ccaccagggc caattgcttc
       61 gccaagcgaa tatgcattaa aggcggcggc aaatccggct tcgactgatt atctgtactt
      121 tattgctggg gatgaaggcg gaggtgcgat gtattttagt aagaccgagg ctgaacacga
      181 acagaacatc aaagatcatt gtcaagtgct ttgtggaact gtttaacaat tcatcaacag
      241 gggtagatgc cttgatttgc ttgaccaaat atgggctatt tggtacgcta gtataagcac
      301 attgccgctc gggttagcgt agactaacag caagtgggaa catcaaaacg ttgcaatggt
      361 gcctaccttt aaatgtcgtt acggacgccg aatagctaaa tatttaatat taattaataa
      421 tatcggtcga cacgagcgct cctgtctcgt ctttgcgaga gaaagacaga gtgaggtaga
      481 aaggatctag taatagatct ctaaggagaa agattattcg taatcaatct gttgacatga
      541 cagttcgtcg gtcatcttta cgcgaatctt ctagtgctca gcagactgca aagtcgagtt
      601 gggcgcgagg ctctatttca ggcactacta ttgccaccta tcttggcatt ttcttgttaa
      661 ttatctcgat gattgctctg gcgtaccatc caccagttag ccaaaaggct cttgccgatg
      721 cctcaaatgg cgcgtcaaca cagcaaaacc agacacagac aacgcaaaat gtctctgttg
      781 accaacttgt ggcaaccgac cttgcatcat cgcttgctga aagcacagat atgcctatta
      841 gtaataacgt tgccaatctt tcgcagacgt tatctgtaga gagcgttttg gcgcagagtg
      901 actctaatgt tattagtaag ccacagatta tccagccaac agctaatggt cgcacgctac
      961 agtactatat agcgggtgta ggcgatactg ttccatcggt tgccgctaaa tacggcattt
     1021 ctgcccagac aatcgaatgg ggcaataatc ttacttcaga agcgctaaca cctggccaaa
     1081 aactgacgat cttgcctact gatggcgtac tttataccgt taaatcagac gacaccctcg
     1141 atagtattgt cgctaagtac ggcgccgacc gttcgagcat cgttgccttt aatgatcttg
     1201 agctaacagg taacccggca gtgggtacac agcttattct acccggcgct gtacttccaa
     1261 acaatgaacg ccctgggtat gtaactccag taacaggcgg aggcggcggt aactatagcg
     1321 gtggctatgg taacgcatac ctagcaaagg cttcggttgg taaccggtac gcatggggca
     1381 actgtacgtg gtatgcctac gagcgacgtc tggagctcgg tatgccagtg ggtagcttct
     1441 ggggtaacgc aagctcatgg gcttcatatg cacgtcaggc cggctatctt gtcgacggta
     1501 atcctacgcc tggcgcagta atgcagaatg gtggaggcta tggtcacgtt gcgatcgtgg
     1561 aatcagttaa cccaggtgtt tcagtgacga ttagcgaaat gaacggatac cgattcggtg
     1621 gtggattcgc ccgcgttggc cacggtgata ttccgtggag cgaagcggta agtggttact
     1681 acaattacat ccactaaacc ctaatttcta acataaataa agcgccccgg ctagcaaaaa
     1741 gggggtaccc tggtaggggc ataacacgat gtcacctcag ggatgtccgt aggcagctat
     1801 cctatttgag ctctgtgtgc tataattaag taatatgttt gtagatactg caaccgtaac
     1861 cgtacaagct ggtaagggcg gcgatggagc tgttagtttt cgtcatgaaa tttatgtcga
     1921 taagggcggt ccagatggcg gggatggtgg taagggcggc gatgttattt ttcttgcaac
     1981 cgagcagtta aacacgctgc ttagctttcg ttaccagcca cgtttgatcg caaacaatgg
     2041 tataaacggc ggcggcacga gaaaagccgg tcgtatgggc aaaccgctaa tcgtgaaggt
     2101 cccggtcggt acgttggtgc gccgtaaagg cgaagtagtc gctgatcttg taaaaccggg
     2161 ccaagaagta gttatcgcaa agggtggtga cggcggattt ggcaatgcac actttaaatc
     2221 cagcgtacgc cagacgcccc gcatcgcaga gctcggagag atgggtgagt cgtttgaggc
     2281 tgagctcgag ctaaagcttt tggcggatgt cgggcttgtg ggatttccaa atgcaggcaa
     2341 gtcaacattc ctatcggttg tgacaaatgc acggcccgag atcgctgact atgcatttac
     2401 tacacttacg ccaaaccttg gtgttgccga catcgacgat ggccatcttc tcatcgctga
     2461 cattccagga ctaatcgaag gtgcaagtga gggtaaaggg cttggcgaca agttcctccg
     2521 tcatgtagag cgtacagcag tgctacttca tcttattgat gcctacagtg atgatatcgc
     2581 tggaagctat atggcaattc gccgtgagct tgaaacatat agtacgcagt taatcaaccg
     2641 cccagaaatt gttgcgctta caaagtgcga tggtttagac gacgatatta ttgaaatgca
     2701 gaagcagtca ctgagggcag taatgcctgt aaatagcgaa atctacgcta tctcttcctc
     2761 ggcgcatcaa gggcttactg agctccttcg tggattacgc cgtgaagtta ccgcagttcg
     2821 tgcgttggcg cctgataatg aagaattgga cgaaacgccg gtaattacgc tgggtgccga
     2881 gtcgttatct gaccactggg aagtaacttt tgatgaagtg aatgccatat atcatataca
     2941 cggtgaaaag attgagaagt tcgcacgccg tacgaactat gagctatacg agaacgttaa
     3001 tcgtttgcgt gatattatgc gccgtcttgg cattacccat gaacttgcac gtatgggtgc
     3061 aagtggcatg agtatggtac aggtcggtga ctcaacaccg ttcaacctga cagagcaata
     3121 gctttgtcgc acgacggact cattattatg cccggtagcg tcctagtgag acaggagggt
     3181 aaaaatgcac gttgcttcat atccgtggtt attctgggtc cactatcggt gtttctctcc
     3241 tagggagaaa gaggacatag tgattcgacc tttgcttttt attgctttta ttgtaaatag
     3301 tattgacttt tctaaatgga agtgttatta tgaaaaacga actttacgtg aggtgcataa
     3361 cc
//
LOCUS       DATIIX010000008         3738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_474581, whole genome shotgun sequence.
ACCESSION   DATIIX010000008 DATIIX010000000
VERSION     DATIIX010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474581"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1894)
                     /locus_tag="VLF64_00180"
     CDS             complement(<1..1894)
                     /locus_tag="VLF64_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90396.1"
                     /translation="MKRLGKSIKGYLLLAVVLCSVFANLFVNYAETHAAADPATLYAA
                     PEVLADAVASEFRTSLALCLTSGGGNNLVDGSGDTLTAKHAAQGPWFADTNEVRPGIL
                     VWGNADDYISCYDLISYNPAKALGLLDNVKLACAMGLVRTDGQDCLTDPKQSDFTDKN
                     IGGDKNKVYANLDAHRTNASAPPTGGWNDAQRYLDVMETMTTFCKGSAIANVGGPLAT
                     PSVAAQYHDSIYNVVDSSGGVTQVYYNVPDHGKKAMLMNYPDNFNWLEVSCGDLAKHS
                     TQYASAYAAYVKTHPQSQASTPTTNPNNGGNDSPPTCTISGIGWIVCPVMRFMSQVTD
                     QAYGVVSDMLTTPALDTGTGSAMYQAWSIMRNFANVAFVIAFLVIIYAQITGFGVSNY
                     GIKKLLPRIIIAAILVNVSYWICAIAVDVSNILGSSLKGLLEGAGKQLYVSGQDFTKT
                     GSPGWDALTVSVIAGTVAVAVLYAGLSVLVPILLMALLAVITVVIVLTARQAIIILLV
                     VISPLAFVAYLLPNTESWFKKWRELFTSLLMMFPIIAVVFGASSLASSVLMQASTTQS
                     TNPTSGFMVMVLHIMAAGVTVIPLAITPVLMKTTTGVLGKIGGFVNNPNKGPFDRMRK
                     GAAGYRE"
     gene            complement(2004..2864)
                     /locus_tag="VLF64_00185"
     CDS             complement(2004..2864)
                     /locus_tag="VLF64_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90397.1"
                     /translation="MTERDYQTRRIQQEGQPEVIPDYVPQSIENNGHGWPTWLKRTVG
                     GVAAATALAAGIAAGVGATSNHNSSKEGQRPAATASATPGETASTTPSSPAETAPVTL
                     EITADSYVFRDQGNTYTGETAFVAAIQKEFEAPTGLEAAQKVPNLLNEWVNSGLNKSE
                     VTQDGMYNMQQVVALYDEAYSTALTGAQYSTLGEDMKSLHAQALTGYAWTHSQPQNEQ
                     DYKMEITGKFFDWMKVNDDTHVNATITATETYSKSTGNNTSQDFPVGFDINTQNGANT
                     FLDWGPRATS"
     gene            complement(3137..3385)
                     /locus_tag="VLF64_00190"
     CDS             complement(3137..3385)
                     /locus_tag="VLF64_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90398.1"
                     /translation="MNSEYDGEWKVYVTTSVEVILHARRANTYRTVTLAPINKLGDVD
                     EQRLDANQLYQFVQQLPNFAHKAGYTLPDEWRRFVDKE"
     gene            complement(3418..3585)
                     /locus_tag="VLF64_00195"
     CDS             complement(3418..3585)
                     /locus_tag="VLF64_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90399.1"
                     /translation="MMDFEAYYTAQEEIARIARDAKLVKQYNRWATAARMAMPQVVAH
                     ARVTPLSNMSD"
BASE COUNT          961 a          854 c          871 g         1052 t
ORIGIN      
        1 tctcacgata tccagcggcg cctttacgca tacggtcgaa aggaccctta tttggattat
       61 tgacaaagcc accgatctta ccaagaacac cagtcgtagt tttcatgagc accggtgtga
      121 tagctagcgg aataacagtt actcccgctg ccataatatg aagaaccatt accataaaac
      181 cgctagttgg gtttgtactt tgcgtggttg atgcctgcat aagaacactg gacgcaagag
      241 agctcgctcc gaacacgaca gctataattg gaaacatcat aagaagagag gtgaagagtt
      301 ctctccattt tttaaaccag ctctctgtat ttggcaacag gtatgcgaca aatgctagag
      361 gtgaaataac taccaaaaga atgatgattg cttgtcgggc ggtaagtacg ataacaaccg
      421 ttataacggc gaggagcgcc atgagtagaa ttggaactag aacggaaagt cctgcgtata
      481 gcaccgcaac cgctaccgta cccgcaataa cgctaacagt aagtgcatcc catcccggac
      541 ttcctgtttt agtgaagtct tgtcctgaaa cgtacaattg tttgccggct ccctcgagga
      601 gacccttaag tgaactaccg aggatattgg aaacatcaac agcaatggcg catatccagt
      661 aggatacgtt tacaagaata gctgcaataa taatgcgagg taataacttt ttaattccgt
      721 agttacttac tccaaagccg gtaatttgcg catatataat aacgagaaat gcaataacaa
      781 atgccacatt tgcaaagtta cgcataattg accatgcttg atacatagca ctaccggtgc
      841 ctgtgtcgag ggcgggtgta gtaagcatat cgctcacgac accatatgct tgatctgtta
      901 cctgtgacat aaaacgcata actggacaaa caatccaacc tattcctgaa atagtacacg
      961 tcggtgggct atcattaccg ccattattcg gatttgtcgt aggggtcgat gcttgtgatt
     1021 gcgggtgtgt ttttacatag gcggcgtatg ctgatgcata ctgcgtcgaa tgtttagcta
     1081 agtctccaca cgatacttct aaccagttaa agttatcagg gtagttcatt agcatagctt
     1141 ttttgccatg atctggaacg ttgtagtata cctgtgttac acccccgcta ctatcaacaa
     1201 cattataaat actgtcatga tattgagcgg caaccgacgg agttgcgagt ggccctccaa
     1261 cgttagcaat cgcactcccc ttacagaagg tggtcatcgt ctccataacg tcaagatatc
     1321 tctgggcgtc attccaacca cccgttggcg gtgctgaagc attcgttctg tgcgcatcaa
     1381 ggtttgcgta taccttattt ttgtcaccac caatattttt atctgtaaaa tcactctgtt
     1441 taggatcagt gaggcaatct tggccatcgg tgcgcaccaa tcccatcgcg caggcgagct
     1501 tcacgttgtc taaaagacca agtgcttttg ctggattgta gctaatgagg tcgtaacaac
     1561 taatgtagtc gtctgcgttc ccccaaacca aaattccagg acgcacttcg tttgtgtctg
     1621 caaaccaggg gccttgagcg gcgtgctttg cagttagtgt atctccggaa ccatctacca
     1681 aattattgcc gccacccgat gtaaggcaga gtgctaatga agtacgaaac tcgctggcga
     1741 cggcatccgc cagaacttca ggagctgcat ataatgtcgc tgggtctgcg gctgcatgag
     1801 tctcggcata gttaacaaaa agattggcaa acacagaaca aagcactacg gcgagtagca
     1861 agtaaccctt tatgctttta cctagtcttt tcatataagt tatgtttaca ttaaacacta
     1921 gcagaaacga aaaagctagt cgatgagata caaaaaacag gccctacttt aaggcctgtt
     1981 ttttcgaatg aaagactata gttctatgac gttgctcgtg gtccccagtc caaaaaggta
     2041 ttagccccat tttgtgtgtt aatatcaaat ccaactggga aatcttgact tgtgttgttt
     2101 ccagtagatt ttgagtaagt ctcggttgcg gtgattgttg cattaacgtg tgtatcgtcg
     2161 ttgactttca tccagtcaaa gaacttgccg gtaatctcca ttttgtagtc ttgttcgttt
     2221 tgtggttgac tgtgtgtcca tgcatatcca gtaagagcct gcgcgtgaag actcttcatg
     2281 tcttcaccaa gggtagagta ttgagctcct gtaagggctg tgctatatgc ttcgtcatag
     2341 agagcaacca cttgttgcat gttatacata ccgtcctgag ttacctcgga tttattaagc
     2401 ccggagttaa cccactcgtt taataggttg ggtacttttt gagcagcttc aagtccagta
     2461 ggagcttcaa actccttctg aatagctgcg acaaatgccg tctcgcctgt ataagtattg
     2521 ccctgatcgc ggaatacgta actgtcggcg gtgatctcta gggttactgg agctgtttca
     2581 gctggagaag atggcgtagt agatgctgtt tctcccggag ttgcggaagc tgtagctgcg
     2641 gggcgctgcc cttctttact actattatga ttacttgtag cgcctactcc tgccgcaata
     2701 ccggctgcaa gtgcagtggc ggcagcaaca ccacctactg ttcgcttaag ccatgttggc
     2761 caaccgtgtc cattgttttc aattgactgt ggaacgtagt caggaataac ctctggctgt
     2821 ccttcttgtt gtattcgtcg ggtctggtaa tctcgttctg tcataatgac gggttcctcg
     2881 cgcacttatg tgggcactta aaacgatttg taatgtttat tatataccca aattactaaa
     2941 aagcaaggtg tctaatatat tttacataca ctacatatgg tgtgtgtagg ttttactcaa
     3001 gataaggcaa tgtgtgtata ttatgtgttg acgagttcgc tgggaggcta gaataatgat
     3061 ttggctataa tctaaaataa cttacccctc gccgtttccg acgagggggc acccgactct
     3121 cgggcggctt gtgtattcac tctttgtcaa cgaatcgccg ccactcatca ggaagagtat
     3181 acccggcctt atgagcgaag ttaggaagtt gctgaacgaa ttgatagagc tggtttgcgt
     3241 caagtcgctg ttcgtcaaca tctccaagct tattgatggg tgcgagggtt acggttcggt
     3301 acgtgttagc acggcgtgcg tgaaggatga cttccacgct tgtggtgaca tatactttcc
     3361 attcaccgtc gtactcgctg ttcacttccc aggcaatgat ctgtcgacca tcaatgttca
     3421 gtcgctcata ttgcttaggg gggtaacgcg agcatgggct acaacctgtg gcatggccat
     3481 cctggcagca gttgcccagc ggttatactg cttgaccagc tttgcatcgc gagcgatacg
     3541 agctatttcc tcttgggctg tgtaataagc ctcgaagtcc atcatccgtc ctttgtccct
     3601 aaacttaact ctgaagtgcg aaagtgcgta gcacttttgc tactctggtg gagcatgagc
     3661 agctacaaac acctcaccaa agtagagcgt gccgagattg gaatattact tcgtaaaggc
     3721 tactccgctg gggatatc
//
LOCUS       DATIIX010000009         7899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_58862, whole genome shotgun sequence.
ACCESSION   DATIIX010000009 DATIIX010000000
VERSION     DATIIX010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7899
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58862"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2639
                     /locus_tag="VLF64_00200"
     CDS             <1..2639
                     /locus_tag="VLF64_00200"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HSW90400.1"
                     /translation="LPVAELPSAITPLANELALSMARAFDEAVEINKTTPITAWRDEW
                     LEKDAVSRFVFKNRKRHAKLRALAQVYFSYISRMEQASLYDYNDMILNVVHAMEIHAD
                     LKANLQEKYQYILVDEFQDTNLAQLRILFNLTDNPLGDEPNVMAVGDDDQAIYSFQGA
                     DVNNINRFREQYPSYRSIVLTHNYRSSEPILRAAREVITQADGRLEAVMDIDKTLTPH
                     ADASSTHVSRTKLPTRMDENDWVARAIAAQIKAGVKPGNIALLARNHDELISLVPHLH
                     HYNIAIDYERRDNILDKESITTLELLAETVVAIFGGDHELADSKLPELLSHPMFAIAP
                     ETIWRLSLQSHRSGLTWLETMLNTPDLRSLAEWLITRAQAVPHETLEVFVDALIGVPS
                     EKTEDYTSPYHQYYFGTNILNGSPEDYLSTLEALRTLRTKLREYQPNDTPHIADMLEF
                     TRMHRELGARIMTIRSGVSNSEAVHLMTSHKSKGLEFDTVFIINSMDSRWGERVRSRA
                     SLINYPENLRIRPNANSYNERLRLYFVAMTRAKRQLFISYSATDTSGKDVLPAGFLSG
                     TTINEQIVTPEHTVETLTILNQLDWQTYVTNRPTATMRALLEPTLENYKLSATHLNNF
                     IDITHGGPIVFLMNNLLRFPAAKSASASYGTAIHATLQRAHNHLVATGEKRPIEDILG
                     DFIHELEHQHLTEMDFEFYSKKGSESLAAFLAAKYSTFARNQRTELSFANQGVFIGEA
                     HLTGSLDLVDFEESSITVTDYKTGRPSNSWKGTTDYDKIRLHKYRQQLMFYQLLCENS
                     RNYSSYSFEGGVLQFVEPDSRGEIHALEESFTHEDLERFSRLIKAVWHCIINLDFPDV
                     SEYDASYKGILAFENALIDKYS"
     gene            2985..3066
                     /locus_tag="VLF64_00205"
     tRNA            2985..3066
                     /locus_tag="VLF64_00205"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3018..3020,aa:Ala,seq:ggc)
     gene            3211..4158
                     /locus_tag="VLF64_00210"
     CDS             3211..4158
                     /locus_tag="VLF64_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HSW90401.1"
                     /translation="MTSFWKTLPQPFFVLAPMEAVTDVVFRHVIAEAGRPDIFFTEFT
                     NTNSWCSPRGKHSTRGRLTFTPDEQPIVAHIWGNVPEYYAEMSRGIAELGFSGIDINM
                     GCPAPNVAPKGSGSGLINTPENAAAIIAAAKVGGLPVSVKTRLGYTRIDEWERWLTHL
                     LQQDIANLTIHLRTRKEMSKVPAHYELIDEIVALRDRIAPETLLTINGDIRDREHGLE
                     LIAKHPGVNGVMIGRGVFHNPFAFALETQPHTREELITLLRHQLDLFDTYESDMRKPF
                     ETLKRFFKIYIRDFDGASELRDALMHTKNTLEVRDLLSR"
     gene            complement(4288..4965)
                     /locus_tag="VLF64_00215"
     CDS             complement(4288..4965)
                     /locus_tag="VLF64_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSW90402.1"
                     /translation="MTVVGRENLPKTGAFILAMGSHTTEVESVIVAAWLRERQLHFYA
                     KSDYWNRPGLKGRLQRWYMSAVGHIPVERGGGRAVLKAIEDGVQVLRQGEVLAVYPEG
                     TRVADGKVHGAYPGTVRTLLDSIRVEREATGVAPHISIIPVGLVHMDSVASPNGGFIP
                     RRGEVMIRIGQPMHLAAAEMTALESGGNRARNTLIEELTSRMMCHIAQLCDKEYDSHR
                     LPIPGSN"
     gene            complement(5144..6070)
                     /locus_tag="VLF64_00220"
     CDS             complement(5144..6070)
                     /locus_tag="VLF64_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90403.1"
                     /translation="MTEKIGIIEMEQTRERKAIVLGRLQDFGLGNPEGIPSSLANASH
                     AELQEIADRDNEALVEPNDFTCTACVDGRKCIHNADGSEPKLRLRRVGGSASNYGVAL
                     NADASIVAVLNPEAPLGEQINTVDDFMGYRSAHLGGCGGAIGEIADHEAIHQKSAVLA
                     AVKAFMAVPEVREYLGVDYTDELGERVRINAGKTAEYLRTMGWDGQKYVDGVKDEVPE
                     NVEDLETDPNDHKFHGHKEAKLTVIIGDKTSAQNDEFVWNLRASKEVAEKLAGQRGEE
                     GYTQAIIAELAKHFAVVDRLPVGELPVILQVA"
     gene            complement(6416..6841)
                     /gene="atpC"
                     /locus_tag="VLF64_00225"
     CDS             complement(6416..6841)
                     /gene="atpC"
                     /locus_tag="VLF64_00225"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HSW90404.1"
                     /translation="MDLQLITLAGVKLDEPVYEVMLPTTSGDISVFPGHEPLVAVAKS
                     GVIAVRRNKQDSDAQLEYFAITGGVIEINTERVRVLVDEAEHGDDIIEAESKAALDRA
                     IALRDSATNQVELEKAHQLVDRHMVRLKVADLQRRHRRS"
     gene            complement(6843..>7899)
                     /gene="atpD"
                     /locus_tag="VLF64_00230"
     CDS             complement(6843..>7899)
                     /gene="atpD"
                     /locus_tag="VLF64_00230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HSW90405.1"
                     /translation="KKASIHREPPALEEQTNKTEILETGIKVIDLIAPIAKGGKVGLF
                     GGAGVGKTVLIQELINNIAKFHSGNSVFAGVGERTREGNDLYHEMSEAGVLDKTSLVF
                     GQMNEPPGARLRVALAGLAMSEAFRDEGKDVLLFIDNIFRFTQAGAEVSALLGRLPSA
                     VGYQPNLQQEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFAHLDSTIVLN
                     RALTEIGIYPAVDPLDSNSTILDPEVVGEEHYNVAREAQRILQQYKELQDIIAILGME
                     ELSDDQKQTVNRARRLQRFLAQPFFVATQFTGNEGVYIKVSDTVKDVKDILAGKYDDK
                     PESWFYMAGGPLSEKKD"
BASE COUNT         2117 a         2217 c         1762 g         1803 t
ORIGIN      
        1 cgctgccagt cgcagaattg ccatccgcga ttacgccact cgccaatgaa ctcgcactta
       61 gcatggcacg cgccttcgac gaagctgtcg aaattaataa gacaacacca attaccgcat
      121 ggcgtgacga atggcttgaa aaggatgctg taagtcgctt tgtatttaaa aatcgtaaac
      181 gccatgccaa gcttcgcgca ctcgcgcagg tatatttttc atatatctcg cgcatggagc
      241 aggcgagtct atacgattac aacgacatga ttcttaatgt cgtacatgca atggagatcc
      301 acgccgatct taaggcaaac ttgcaagaaa aataccaata cattttagtg gacgaattcc
      361 aagacactaa cctcgcacag ctacgtattt tattcaatct caccgacaac ccgcttggcg
      421 acgagccaaa tgttatggcg gttggtgatg atgatcaggc aatttatagc ttccagggcg
      481 ctgacgttaa taatatcaac cgttttcgcg agcaataccc gagctatcgt agcattgtgc
      541 taactcataa ctatcgatca agtgaaccaa ttttgcgagc cgcgcgtgaa gtaatcacgc
      601 aggctgatgg ccgacttgag gccgtcatgg acatcgacaa aacactcacg ccacatgccg
      661 atgcaagcag cacgcacgta agtcgcacga agctcccaac gcgcatggac gaaaatgatt
      721 gggtagcaag ggcaatcgca gcacaaatta aagcaggcgt aaaaccaggc aatatcgcac
      781 tactcgcacg caaccacgac gaacttatct cactcgttcc acacctccat cactacaata
      841 tcgctatcga ttacgagcgt cgcgacaaca ttttagataa agaaagtatc acgacacttg
      901 agctactcgc cgaaaccgtc gtagcgattt tcggcggaga tcacgagctc gccgacagta
      961 aactacccga gttgctttca catccaatgt ttgcgattgc gcccgaaaca atctggcgac
     1021 taagtctgca gtcccatcgt agtggactca cgtggctcga aacaatgcta aatacacccg
     1081 atctgcgctc gctcgccgag tggcttatta cacgcgccca ggctgtgcca cacgagacgc
     1141 tggaagtatt tgtcgacgca ctcattggcg tgccaagcga aaaaacagaa gactatacgt
     1201 cgccatacca tcaatattat tttggcacca acatacttaa tggttcgcca gaggattacc
     1261 tctcaacact cgaggcactt cgcacactgc gcaccaaact acgcgaatac caaccaaacg
     1321 atacgcccca tatcgctgac atgcttgaat tcacacgtat gcaccgcgaa cttggcgcac
     1381 gtatcatgac tattcgcagc ggcgtcagta atagcgaagc cgtgcatctt atgacatcgc
     1441 ataaatcaaa aggccttgaa ttcgatactg tattcatcat caactccatg gatagccgct
     1501 ggggcgagcg cgttcgtagc cgtgcgtcac tcattaacta tccagaaaat ctccgcattc
     1561 gcccaaacgc aaactcatat aacgaacgcc tacgtctcta tttcgtagcc atgacccgcg
     1621 ccaagcgcca gctgttcatc agctacagtg ctactgacac aagcggcaaa gatgtactac
     1681 cagctggctt cctaagcggt accacaatca acgaacaaat cgtcacaccg gaacataccg
     1741 tagaaacact cacaatactc aatcaacttg attggcaaac ctatgtgaca aatcgaccga
     1801 ctgcaactat gagagcactc cttgagccga cgctcgaaaa ctacaagctt tcagcgactc
     1861 atcttaataa ttttatcgat atcactcatg gtggacccat agtcttcctt atgaacaatc
     1921 ttctcagatt cccggcagca aaaagcgcta gtgcaagtta tggcacagct attcacgcta
     1981 cattgcaacg cgcccataac catcttgttg cgacaggcga aaaacggcct atcgaagata
     2041 tactcggaga tttcattcac gagctagagc accagcacct cactgaaatg gattttgaat
     2101 tctacagcaa gaagggcagc gaaagccttg ccgcattcct tgcagcaaaa tatagcacat
     2161 tcgcacgcaa tcagcgcacc gagctcagct ttgctaatca aggcgtattc attggcgaag
     2221 cccatcttac cggatcactc gaccttgtcg attttgagga atcatccatc accgtaaccg
     2281 actacaaaac cggtcgccca agcaatagct ggaagggaac gacggattac gacaaaatcc
     2341 gtctacataa ataccgccaa caactcatgt tctaccaatt actctgcgaa aactcacgta
     2401 actattcgtc gtatagcttt gaaggcggtg tgctgcaatt tgtcgagcca gatagccgtg
     2461 gagaaatcca tgcacttgaa gaatcattca cacacgaaga cctcgaacgc ttttcccgtc
     2521 tcatcaaagc tgtttggcac tgcattataa acctggattt cccagatgtc agtgagtatg
     2581 atgcaagcta caaaggtatt cttgcgttcg aaaatgccct cattgacaaa tatagctaga
     2641 tagtttagtg ttatagtggt caacggaccg cactccacca cgattcggac gaggtgaaat
     2701 tcatgccagt cacggagggt gacgcgcgaa agcgcggtac gtcccggtgt gccggctgct
     2761 gcccggcaac tcactacggg agaagtccga gctgcagcca atgtgggacg gtgcgcggga
     2821 gtacgtctcc cgcaaccaac cacgtacact catgcgcagc ccacccgggc aatcgcaggt
     2881 aacgagacca gggtattgcc agcacggcca ctatgtggta aagtagtacg gcggtccacg
     2941 cgtctaccgg tgattcactc ccggacgatg cggaggaccg tccagcggcg gaagctcaac
     3001 tggcagagcg atcccatggc aaacgcctag cgggataggt tgaaggttcg aatccttccc
     3061 gtcgcacttg gccctaacca acgggccttc tccggagtta cgtcacagcc tacaggcagt
     3121 gactccccgt actccggttt accctcactc cttgacgcca attcagcgtc ttggggtggg
     3181 ggtttatcca ttttatgaga taatatacta atgacatcat tctggaaaac tctaccgcaa
     3241 ccattctttg ttctggctcc tatggaggca gtgactgatg tggtctttcg ccatgttatc
     3301 gccgaagcag ggaggcccga tatattcttc acagaattca ccaataccaa tagttggtgt
     3361 agtccgcgcg gcaaacacag cacccgtggc cgtctaacat tcacccctga cgaacaacca
     3421 atcgttgcgc acatctgggg taacgtacca gaatactatg ctgagatgag ccgtggtata
     3481 gccgagcttg gattttctgg tatcgatatc aatatggggt gtccggcacc aaacgtcgcg
     3541 ccgaagggct cgggcagtgg gcttattaac acacccgaaa atgccgcagc aatcattgca
     3601 gcggcaaaag ttggtggcct gccagtgagt gtcaaaactc gccttggcta cacgcgcata
     3661 gatgaatggg aaaggtggct tacgcacttg ctacaacaag acatcgccaa ccttacaatt
     3721 cacctgcgca cgcgtaaaga aatgagcaaa gtgccggcac actacgaact tatcgatgaa
     3781 atcgtggcac ttcgtgatcg tatcgcaccc gagactctac ttactatcaa cggggacatc
     3841 cgcgatcgcg agcacggcct tgagctcatt gccaagcatc caggtgtcaa cggcgtcatg
     3901 attggtcgcg gtgtattcca taatccattt gcatttgcac tcgaaactca gccacacacc
     3961 cgagaagaac tcatcacact cctccggcac cagcttgacc tgtttgatac atacgaatca
     4021 gacatgcgta aaccattcga aaccctcaaa cgattcttca aaatctacat ccgcgacttc
     4081 gacggtgcca gcgaactccg cgatgcatta atgcatacaa aaaacacctt agaagtacgc
     4141 gacctactta gtcgctaatc gactcgcagt atactacaaa aaatacggac aaagtccata
     4201 gatcatttca gttcttccat acaagcagaa gtgaaagagc gctcgggcgc caggctcggc
     4261 cattatagct ccgcctggcg ccctccgtta gttgctccca ggaatcggca gccggtgcga
     4321 gtcgtactcc ttgtcgcaga gctgcgcgat atgacacatc atgcggcttg tgagctcctc
     4381 tatcaaagtg ttccgagccc gattgcctcc actctcgagg gctgtcatct cggccgcagc
     4441 caaatgcatc ggctggccga tgcgaatcat tacctcgcca cgccgcggga tgaatccacc
     4501 attcggcgag gccacagaat ccatatgaac gagcccgacc gggatgatcg aaatgtgcgg
     4561 agcaacgccc gtcgcttcgc gctcaacacg gattgagtca agcagcgtgc gcaccgtgcc
     4621 cgggtaggca ccgtgcacct taccgtccgc cacacgggta ccctcaggat acaccgccag
     4681 tacctcacct tgacgaagca cctggacacc gtcctcgatg gccttcagta ctgcgcgacc
     4741 tccgccgcgc tccactggaa tgtgcccgac agcactcatg taccagcgct gcaatcggcc
     4801 tttcagacca ggtcgattcc agtaatcaga cttcgcgtag aaatggagct gtcgctcccg
     4861 cagccacgcc gcgacgatta ctgattcaac ctcagtcgta tggcttccca tcgccagaat
     4921 gaacgcaccg gtcttcggca ggttctcacg gccgacgaca gtcacacgaa cccgatatct
     4981 gataaacggc ccgaacagca cgctattgag caagccgtac agattcaacg ttcttcacct
     5041 cctgcttgta aaagggccac gtccgaatga cacagcctgt gtatctatta tgacataaaa
     5101 tactactcat acaaatgagc agtattttat aaagaagtgc tttttatgca acttgcaaaa
     5161 tgactggcaa ttcaccaaca gggagacgat caaccacagc aaagtgttta gcaagctccg
     5221 caataattgc ctgtgtgtat ccttcttcgc cgcgctgacc ggcaagcttc tcagcaacct
     5281 ctttacttgc gcgaagattc caaacaaatt catcattctg ggcagaagtc ttgtcaccga
     5341 taataacagt aagcttcgct tctttatggc cgtgaaactt atgatcgttg ggatcagttt
     5401 cgaggtcttc aacgttttca ggtacttcat ccttcacgcc gtcgacgtac ttttgaccat
     5461 cccaacccat tgtgcgtagg tattccgctg tcttaccagc gttaatacgc acacgctcgc
     5521 caagctcatc agtatagtca acaccgaggt actcacgaac ttccggcact gccatgaacg
     5581 ctttaaccgc tgcaagcact gccgacttct gatgaatagc ctcatggtcg gcaatctcac
     5641 caattgcgcc accacaacca ccaaggtgtg ccgagcgata tcccataaag tcatcaactg
     5701 tattaatttg ctcacccagt ggagcttcag gattaagcac tgcaacaatt gaggcatccg
     5761 cgtttaacgc gacaccatag ttcgatgctg acccaccaac acgacgaagg cgaagttttg
     5821 gctcagaacc atccgcgtta tgtatacact tacgaccatc gacacatgca gtacacgtaa
     5881 aatcgtttgg ctcaacaaga gcctcgttat cgcgatcggc aatttcctgc agctcagcat
     5941 gtgacgcatt cgcaagtgaa gagggaattc cttcagggtt accaagtcca aaatcttgca
     6001 acctgccaag aacaattgcc ttgcgttcgc gtgtctgttc catttcaata atacctattt
     6061 tttcagtcat acctattact ctattatttt cctcagctaa ttgtactctt cgcgctacag
     6121 atgtaaaggc catacacgtg ctacaataaa aatatgagcg agcaaggcag attctccagc
     6181 ctcccgcgcg tcgaacaaat cgaccttaat acaattttgc cagatgacat tatcgatcca
     6241 cagcataccg gccggtggct tgagttcgca gagcaaaacc cggagcttgc gaatgaaatg
     6301 cttaaacgag cttttggttt agccagaaca gcacaaaact cccttgagat atccaaggga
     6361 gttcttgatg caataacctt tgccgcgagc gcacttgccg aggcgtttag acgcactacg
     6421 aacgtcgatg tcgacgctgc agatccgcaa ccttcaggcg taccatatga cggtcaacca
     6481 actgatgagc cttctcgagc tcaacctgat tcgtagcact atcacgtaat gcaatggcac
     6541 ggtcaagcgc agccttactc tcggcttcga tgatatcgtc accatgctcg gcttcatcaa
     6601 ctaatacgcg cacgcgttcg gtgttaattt cgataactcc acctgtaatc gcaaagtatt
     6661 caagctgcgc gtcactgtct tgcttgttgc ggcgaactgc aatcacacca gactttgcaa
     6721 ccgcaacaag tggttcatgg ccaggaaata cagagatatc accactcgtt gtcgggagca
     6781 tcacctcata tacaggttcg tccagcttaa caccagcaag agtaatgagc tgcaaatcca
     6841 tactagtcct tcttctcgct tagtgggccg ccagccatgt agaaccagct ttcaggcttg
     6901 tcgtcgtact taccagcaag gatgtctttt acatctttca ctgtgtcaga caccttaata
     6961 tacacacctt cgttaccggt aaactgagtt gcaacaaaga atggctgcgc caagaagcgt
     7021 tgcaagcgac gcgcgcggtt cacagtttgc ttctggtcat cagaaagctc ttccatgcca
     7081 aggattgcaa taatatcttg caattctttg tattgctgaa gaatacgctg cgcttcacga
     7141 gctacgttgt aatgctcctc accaacaacc tctgggtcta agatagttga gttactatca
     7201 agtgggtcga ccgcagggta gatgccaatt tcagtaagtg cacggttcaa cacgattgtt
     7261 gagtcgaggt gggcaaacgt ggttgcaggc gctgggtcgg taaggtcgtc ggcaggcacg
     7321 tatacagcct gaacagaagt aatcgaaccc ttcttagtag aagtaatgcg ctcctgcaaa
     7381 gcacccattt cttgctgcaa gtttggctgg taaccaaccg cgctcggtag gcgtccaagc
     7441 agcgctgaaa cctcagcacc agcctgggta aagcgaaaga tgttatcgat aaagagcagc
     7501 acatccttcc cctcgtcacg aaaggcttct gacatcgcaa gccccgcaag cgctacgcga
     7561 agacgcgccc caggtggttc gttcatctgg ccgaatacaa gcgacgtctt atcaagcaca
     7621 cctgcttcgc tcatttcgtg ataaaggtcg ttaccctcac gggtacgctc accaacacca
     7681 gcaaatacag agttaccact gtgaaactta gcgatgttgt taataagctc ctgaatcagg
     7741 acagtcttac caacaccggc gcccccaaag agtccaacct taccgccctt cgcgataggt
     7801 gcaataaggt cgataacctt aataccggtt tcaaggattt cagttttgtt tgtctgctcc
     7861 tcaagtgcag gtggctcacg gtgaatcgag gccttttta
//
LOCUS       DATIIX010000010         6297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_225640, whole genome shotgun sequence.
ACCESSION   DATIIX010000010 DATIIX010000000
VERSION     DATIIX010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225640"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..331)
                     /locus_tag="VLF64_00235"
     CDS             complement(56..331)
                     /locus_tag="VLF64_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HSW90406.1"
                     /translation="MSTITDTTTDGTNVIIYSTPTCAFCKTEKQYLDHLGVPYIVKDV
                     EEDAGAMAELIAKSSQQAVPVTDIDGVIIRGFDRLKIDETLKEKALV"
     gene            436..2616
                     /locus_tag="VLF64_00240"
     CDS             436..2616
                     /locus_tag="VLF64_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ricin-type beta-trefoil lectin domain protein"
                     /protein_id="HSW90407.1"
                     /translation="MRIFRAHQGFALPTVVITSVVMFAILTAVMGMVASTSSNLDIQY
                     YEGIAADAAESGAAHANDCLKNNGYVSSWGVKTLTPATDCTGTVVSGQAAYIVSTSSY
                     RTSYSVSPVTSSNVGTQTATITGTTSMLRPSGTVWKTYAKTLIVKTGGQISASQVVFG
                     YSSGAGAFFGTVGADGVMRTVGYNGQGQLGNGGYSSTLTPQVFQVPGGARIVSGFTSF
                     VSLGIHMYALDSNGNAYGAGWNGHGGELGVGQPSSTSSIPTPAKVVLPGKQISFIMVG
                     YNNNYFLTTDNNVYAAGACANGLLGSNYTISGCVDQSAPVRVNLPTPTGSDPNTIPTN
                     NITTDRDDVYVRMAGGRVYGWGANYWAQLGGDNFVPSSTPVQIGTYGDPGQPKATQVF
                     TDGSSMYILDSTGHVNSLGLNNYGQMGTNTTSLVMAASTGKCVDNENGDGVTFWLFPC
                     NGTPAQKYQLRSDGSVYNAATGRCLDNSNADGVSLHLNSCNGSAAERFVWDANAGTLY
                     NAQANKCVDNKYGDGTTLWLYSCIGSINQAFTAYNGNLTSFDMSGFTGTITKIWADQG
                     SLSALTTKGEIWSAGYNTAGQFGNGSTATYQSTPLKFNMPVAATDLYESLSSNTNVYQ
                     NLYAVGSNGRIYGAGANTFGQLGNGTTGAVVSTPVVMSVIDGSSIVASQVKTGYGTTV
                     VFTTNGSVYTVGNNGSGQLGDGTTTNRSTPIKAQYVNNLAPTTY"
     gene            complement(2603..2800)
                     /locus_tag="VLF64_00245"
     CDS             complement(2603..2800)
                     /locus_tag="VLF64_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90408.1"
                     /translation="MPRRNNTPKHIPYESKTAAAPLKKRFASRQAALQAIKELQKYHL
                     DLELSTYQSPVDGGWYLTSTS"
     gene            2855..3124
                     /locus_tag="VLF64_00250"
     CDS             2855..3124
                     /locus_tag="VLF64_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90409.1"
                     /translation="MSKLPNDSTSAGDGNKRSRRSDGLYLIASMADTTWRLFIPTLGL
                     LLSGNALDDKLHTHPWLMLAGAILGGWIAAVLIKRQIAKGNDSAK"
     gene            3114..3932
                     /locus_tag="VLF64_00255"
     CDS             3114..3932
                     /locus_tag="VLF64_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HSW90410.1"
                     /translation="MLNSFAASDIHVKLPVEPIFHVGSFPVTNSMILGTFGIVVMLAV
                     FAYTVSALKHGKHNHFVGLVQWAVEGMYSSVLDIIPDRKVARSIAPLALGMFFTVLFT
                     YWCAILPGVASVTLDGKELFRELPSDLNFTFALAIISLVAAQYYAIKTHGVVGNIKRY
                     AKNPLKDPIGWFEGLLEFIGEFSRYIALSLRLFGNAFAGAVHLMIVGVLTGWLAGLTL
                     PFVMIFELFIGFIQAYVFFVLTVIFTALATASHGDSHSSDHSLAEPKLAESDRT"
     gene            3965..4174
                     /locus_tag="VLF64_00260"
     CDS             3965..4174
                     /locus_tag="VLF64_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HSW90411.1"
                     /translation="MNALAFGLTYALPAFGAAIGVGLIGLGALNALGRNPEKLSDIRT
                     LMITAIVFADSLAIIGIIVAFIAKA"
     gene            4184..4717
                     /gene="atpF"
                     /locus_tag="VLF64_00265"
     CDS             4184..4717
                     /gene="atpF"
                     /locus_tag="VLF64_00265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HSW90412.1"
                     /translation="MASFIGQFAAEGSSDIMTGLGIDGKLLVFQLVAFLLLVFVLSKW
                     VFPIFFKIIDKRQETIEESNRAAVEASKHAEKAQEEIAKLLKEARTEAKAIVTTAKEE
                     ATAMVQDAESKGKAQAEHIVAEAHDEIAKEVIAAKKALHNETIDLVALATEKVIGKTV
                     TDGVDGKVIKAALEESR"
     gene            4721..5104
                     /locus_tag="VLF64_00270"
     CDS             4721..5104
                     /locus_tag="VLF64_00270"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HSW90413.1"
                     /translation="MQGKISRRKLALYVADAIEAKQKLGAILSEVAAYLVDSKRTREA
                     ELLVRAIEDELAARGIVIAHVVTARPLAKELEQAITKMIDAREIHLDAEVDPSVIGGV
                     RVEMPGKLLDATIKRKLLALHQAKI"
     gene            5118..>6297
                     /gene="atpA"
                     /locus_tag="VLF64_00275"
     CDS             5118..>6297
                     /gene="atpA"
                     /locus_tag="VLF64_00275"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HSW90414.1"
                     /translation="MADIAVTELSKGLRAAIAKLDASDNLESVGVVTRVGDGVAWVHG
                     LADAGYSEVLQIETKSGIIEAFVLNLMEDEIGAVLLGSDQTVSAGDKVTLKGEVLSVP
                     VGPELLGRVVNPLGEPLDGGPAIKAKQHGTVEREAIGVMGRKHVHEPMMTGIMAIDAM
                     FPIGRGQRELIIGDRQTGKTAIAIDTMINQARENTGVVNIYVAVGQKLSKVAGLVERL
                     KEEGVMDQTIVVVTGPSDPASLLYLAPYAGTAMGEYFRDNKQHALMIYDDLTKHAVAY
                     RQMSLLLRRPPGREAFPGDVFYLHSRLLERSAKLSDDLGAGSLTALPIIETQAGDISA
                     YIPTNVISITDGQIFMETDLFYQGIRPAISAGLSVSRVGGDAQTKAVKSVSGHLKLGL
                     S"
BASE COUNT         1541 a         1352 c         1635 g         1769 t
ORIGIN      
        1 attggcaaaa aataatcccc acgatttcgc tggggattat ttttgaaagg cgattttata
       61 cgagtgcttt ttctttgagt gtttcatcga ttttgaggcg gtcgaagcca cggataatta
      121 ctccatcgat gtcggtaacg ggcactgctt gctggctgga ctttgcgata agctctgcca
      181 ttgcgcctgc atcttcttca acatctttta caatgtatgg tacgcctaaa tggtcaaggt
      241 attgcttctc tgttttgcag aatgcgcacg taggtgtgct gtagataatg acatttgtgc
      301 catctgttgt cgtatctgtt attgttgaca tatctccccc tttatttcta gtgtccatta
      361 taccaacagg cacactagga ttcaagccat aatcgtcatg gctaaatgat tttacgtgta
      421 gtaaaataaa agcatatgcg gatatttagg gcgcatcagg gtttcgcgct cccaacggta
      481 gtgataacat cggtcgtgat gtttgctatt cttactgctg taatgggtat ggttgcgagt
      541 acgagtagta atcttgatat tcagtactat gaaggtattg ctgcggatgc agctgagtcg
      601 ggtgcggcgc acgctaatga ttgtcttaag aataacggat atgtgagttc gtggggagta
      661 aagacgctta ctcctgcaac ggactgtact gggacggtag tttctggcca agcggcttat
      721 attgtctcaa cttctagcta tcgaacgtcg tatagcgtaa gccctgtgac aagttcgaat
      781 gtaggcacgc aaacagccac gattaccggt acgacatcca tgcttcgccc ttcgggcact
      841 gtttggaaaa cgtatgccaa aacactaatt gttaaaacgg gtggtcagat tagtgctagc
      901 caagtagtat ttggctactc gtctggtgcg ggtgcattct ttggtacggt tggtgctgat
      961 ggcgtcatgc gaaccgtggg ctacaacggt caggggcagc ttggtaatgg aggatacagc
     1021 tcaaccctta ccccacaagt atttcaggtc ccgggagggg ctcggatagt ttccggcttc
     1081 acaagctttg tatcacttgg tatccacatg tacgcgcttg atagtaacgg gaatgcatac
     1141 ggtgccggat ggaatggtca cggtggcgag ctaggcgtgg gacagccttc atcgacttct
     1201 tctataccga cacctgctaa agtggtgctt cccggtaaac agatcagttt tattatggtt
     1261 ggttataaca acaactattt cctcacaaca gataataatg tatatgcagc gggggcgtgt
     1321 gcgaatggtc tacttggctc aaactatact atttcaggct gcgttgacca atcagctccc
     1381 gtacgtgtga atctgccaac gccaacaggc agcgatccta acacaattcc aactaacaat
     1441 attactactg atcgtgacga cgtatatgtt cgcatggctg gtggcagggt atatggttgg
     1501 ggggcaaatt attgggcgca attgggcggc gataattttg taccttcttc aactcctgtg
     1561 caaattggta cgtatggtga tcctggccag ccaaaagcga cgcaggtatt tacggatggc
     1621 tcctcgatgt atattcttga tagcacagga catgttaata gtctagggct taacaactat
     1681 ggccagatgg gaacgaatac tacgtcactt gtaatggctg caagtactgg caaatgtgtt
     1741 gataacgaaa acggtgatgg tgtgactttt tggctctttc cctgtaacgg cacgcctgca
     1801 cagaagtatc aattacgatc agatggctca gtatataatg cggctacggg tcgttgtctt
     1861 gataatagta acgctgatgg cgtttcgctc caccttaata gctgtaacgg tagtgcggca
     1921 gagaggtttg tctgggacgc taatgcgggt acattatata acgcgcaggc taataaatgc
     1981 gttgataata aatatggtga cggcacgaca ctgtggctct attcctgtat tggctcgata
     2041 aaccaggcat ttacagccta taacggtaat cttacatcat ttgatatgtc aggtttcacg
     2101 gggacaatta ctaaaatatg ggcagaccaa ggatctttaa gcgcgctaac aacaaaaggt
     2161 gagatttgga gtgcaggata taatactgct ggtcagtttg gtaatgggtc gacggcgact
     2221 tatcagtcga cgccactgaa attcaatatg cctgtggctg caacagacct gtacgagtct
     2281 ctcagtagca atacgaatgt ctaccagaat ctctacgcag tgggctctaa tggcagaatt
     2341 tatggcgctg gggctaacac atttgggcag cttggtaatg gtacgacagg cgcagtggtt
     2401 tctacgcccg ttgtgatgtc ggttatcgat ggaagctcaa ttgttgcgtc acaggtaaag
     2461 actggatatg gtacaacggt tgtatttacg actaatggat cggtgtatac cgtgggtaat
     2521 aatggctcag ggcaactagg cgacggtacc acgacgaaca gatcaacccc tattaaggcg
     2581 caatacgtca ataaccttgc acctacgacg tactagttaa ataccagccg ccatcaactg
     2641 gcgactgata cgtgctgagc tcaagatcga gatggtactt ttgaagctct tttattgcct
     2701 gtaaagccgc ttgacgactt gcaaaacgct ttttgagcgg tgctgccgca gtttttgact
     2761 cgtatggaat gtgttttggg gtattattgc ggcgcggcat tttcttaaaa gtagccctta
     2821 cattagtggt ggatatctgg tacaataata ccatatgagc aagctgccaa acgacagcac
     2881 tagtgccggt gacggtaaca agcgatcgcg acgatctgac ggtctctacc tcatcgcatc
     2941 tatggcagat acaacatggc gcctgtttat accaaccctt gggctcttgc tcagtggaaa
     3001 tgcacttgat gacaagctac acactcaccc gtggttgatg cttgctggtg caatccttgg
     3061 tggttggata gcggctgtat taataaagcg acaaattgca aagggtaacg atagtgctaa
     3121 atagtttcgc agcaagtgac attcatgtaa aactccccgt ggagccaatt ttccacgttg
     3181 gttcattccc tgtaactaat tcaatgattc ttggtacctt tggtattgtc gtgatgcttg
     3241 cggtgtttgc ctacacagtt tcggcattga aacatggtaa gcataatcat tttgtggggc
     3301 ttgtgcagtg ggctgtcgaa ggcatgtata gctcggtgct ggatattatc cctgaccgta
     3361 aagtggccag gagtattgct ccgcttgcac ttggtatgtt ctttaccgtc ctctttacgt
     3421 actggtgtgc gattttacca ggcgtggcgt cagttaccct tgatggcaaa gagctctttc
     3481 gtgaattgcc tagtgacctg aacttcacgt tcgcacttgc gattatttcg cttgtcgcgg
     3541 ctcagtacta cgcaatcaaa acacatggtg ttgttggtaa cattaagcgt tacgcaaaaa
     3601 atcctttgaa ggaccctatc ggatggttcg aaggcttact tgaatttatc ggtgaattta
     3661 gtcgctatat tgcactgagc ttacgtttat ttggcaacgc gtttgcaggt gctgtgcatc
     3721 tgatgattgt tggtgttttg actggctggc ttgctggtct tactctaccg tttgtgatga
     3781 tatttgagct gttcatcggt tttatccagg cgtatgtatt ctttgttctg acggttatct
     3841 ttactgccct cgccacggca tcgcatggcg actcccattc ttctgatcat tctcttgccg
     3901 aaccgaagct agcagagagt gaccgtacgt aacgtttatt aataaataag gagatttact
     3961 aactatgaac gcattggcat ttggtttgac ctacgcactt ccagcatttg gtgctgcaat
     4021 cggtgttggt ttgattggtc ttggcgcgct taacgcactt ggccgtaacc cagagaagct
     4081 tagcgatatc cgtactctta tgattactgc tatcgtgttt gctgactcac tcgcgatcat
     4141 cggtatcatt gttgccttca ttgcaaaggc gtaatggtaa tttatggcaa gttttattgg
     4201 acaatttgcc gctgagggta gctcggatat catgacgggc cttggtattg atgggaagct
     4261 tcttgttttc cagcttgttg cattcttgct tttggtgttt gtactttcga aatgggtatt
     4321 ccctatcttt ttcaagatca ttgataagcg tcaagagaca atcgaagaga gcaatcgtgc
     4381 tgccgtagag gcatctaaac atgcagaaaa ggcacaagaa gagatcgcta agcttctcaa
     4441 ggaggcgcgc accgaagcga aagcgatcgt gacgaccgcc aaagaagaag ccactgctat
     4501 ggtgcaggac gctgaatcta agggcaaggc acaagccgag cacattgtgg ctgaagcgca
     4561 tgatgaaatt gctaaggaag tgattgcggc gaagaaagct cttcataacg agacgattga
     4621 tcttgttgca ttagcaactg aaaaagttat tggtaagaca gtaactgacg gtgtcgatgg
     4681 taaggtgatc aaagcggcgc tcgaggagag caggtaatct atgcaaggta aaatctctcg
     4741 ccgtaagctc gcgctttacg ttgctgatgc aatcgaggca aagcagaagc ttggagctat
     4801 tctttctgag gtagctgcgt atcttgtcga tagtaaacga acgcgcgaag cagaattgct
     4861 tgtgcgtgct atcgaggatg agcttgcggc acgcggtatt gttattgccc atgtagtaac
     4921 ggcacgacca ctcgcgaaag agcttgagca agcgattacc aagatgattg atgctcgaga
     4981 gattcacctt gatgcagagg ttgacccgag cgttatcggt ggcgtacgtg ttgagatgcc
     5041 gggcaaatta ctcgatgcga ccattaaacg taaactcctg gcattacacc aggcaaagat
     5101 ataagaaagg ctgactgatg gctgatatag cagtgacaga attatcaaaa ggccttcgcg
     5161 cggcaattgc taagttggat gcgagcgata atctcgaatc agttggcgtt gttactcgtg
     5221 taggcgacgg tgttgcgtgg gttcatggcc tcgcagatgc tggctacagc gaagtgcttc
     5281 agattgagac caaatctgga attatcgaag cattcgtact taaccttatg gaagatgaga
     5341 tcggtgccgt actcctcggt agcgaccaaa ccgtttctgc aggtgacaaa gtaacgctta
     5401 agggcgaggt actttctgta ccagttggcc ctgaacttct tggtcgtgtg gttaaccctc
     5461 tcggcgagcc acttgatggt ggcccggcga ttaaagcaaa gcaacatggt accgtagaac
     5521 gcgaagcaat tggcgtgatg ggccgtaaac atgtgcacga accaatgatg accggcatta
     5581 tggcaatcga cgccatgttc cctatcggtc gtggtcagcg tgagctgatc attggtgacc
     5641 gtcaaactgg taagacggca atcgcgatcg acactatgat taaccaggct cgtgaaaaca
     5701 ctggcgtggt aaacatttat gttgcagttg gccagaagct ttctaaggtt gctggccttg
     5761 tcgagcgcct taaagaagaa ggcgtgatgg atcagacgat tgtggttgta actggcccaa
     5821 gtgatcctgc atcactcctt taccttgcgc catatgcggg tacggctatg ggtgaatatt
     5881 tccgcgacaa caagcagcac gcgttgatga tttacgatga cctcacgaag cacgcggtcg
     5941 cataccgcca gatgtcattg ttgcttcgtc gtccacctgg acgtgaagcg ttccctggtg
     6001 acgtgttcta tttgcactct cgccttttag agcgctcggc gaaactttct gatgacctgg
     6061 gtgcaggttc gctcactgct ctccctatta ttgagacgca ggctggtgat atctctgcat
     6121 acatccctac taacgtgatt tcaatcactg atggccagat ttttatggaa actgacttgt
     6181 tctaccaagg tattcgtccg gcgatctctg caggactttc ggtgagtcgt gttggtggtg
     6241 atgcccagac taaggctgtt aagtcggttt cgggtcatct caaacttggc cttagca
//
LOCUS       DATIIX010000011         2177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_336934, whole genome shotgun sequence.
ACCESSION   DATIIX010000011 DATIIX010000000
VERSION     DATIIX010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336934"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..2139
                     /gene="ligA"
                     /locus_tag="VLF64_00280"
     CDS             106..2139
                     /gene="ligA"
                     /locus_tag="VLF64_00280"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HSW90415.1"
                     /translation="MPSRPNLQKARERVEKLRVAINDYRYHYHVLDESTMSEAAADSL
                     KHELSQLELEYPELVTPDSPTQRVAGKALDKFTKVTHRTRMISLADVFNDEEIVAWIE
                     RMQKALPNVSNEYLCDIKMDGLACSLVYQDGVLQQAVTRGDGLVGEDVTMNVRTIKNV
                     PLRLRGNQKFAAFLRGRTEIRGEIVMYKQDFAKLNEQRKEAGESLFKNPRNLAAGTIR
                     QLDPQLVAARPLHFVGYDIIRDDWDDVPTIAYGYSAMYELGIETSKQTQVVQGKAAII
                     DYVTHLGEIRGDLKFNTDGAVIKLNDRAQFEDLGIVGKTPRAAVAFKFAAEEATTIVK
                     DIVISIGRTGAATPVAVFDPVQVAGTTVQHASLHNADEIARLDVRRGDTVIIFKAGDI
                     IPQVQSVVPELRPKDSAAISYEEELKRQYPELEFVRPEGEVVYRVKGLTGDLILKRSL
                     EHFVSKGALDINTFGEKNVVAVIDAGYVNNLADIYRLTKEKLLQLDRFADISAAKLID
                     AVAAAKNPPLERFVYGLGIRHVGVQTAIDLVSHFGSLDAITHATIDELEGIDGIGKVV
                     AESIAAWFADEDNLMLLDEFTKLGVAPHFEKKTGKLLGQNFVVTGTLESMGRDEAADA
                     IRALGGTFQTAVAKDTTYLVAGGKIGASKLKKAEQYGTKIITEAALLELIKNK"
BASE COUNT          603 a          478 c          557 g          539 t
ORIGIN      
        1 cttggctgcc ggtcgttccg tatgtaggtg cgctggtaac ggcatatgct gtggtgtggt
       61 acgcaatgta cgcatacctc ttttacagaa agatagtaga cgacgatgcc aagccggcct
      121 aatttacaaa aagcacgtga gcgtgttgaa aagctgcgtg ttgcgattaa cgattatcgc
      181 tatcactatc acgtactcga tgaatcaacg atgagcgagg ccgcggcgga cagcttaaag
      241 cacgagcttt cgcagcttga attagaatac ccagagctcg tcacacccga tagcccaacc
      301 cagcgcgttg caggcaaggc gcttgataaa tttacgaagg tgacacatcg cacacgaatg
      361 attagtttgg cggatgtctt caatgatgaa gaaattgtcg cgtggatcga acgtatgcag
      421 aaagcgttgc cgaatgtatc aaatgagtac ctctgtgata tcaaaatgga cggtttagca
      481 tgctcgctgg tataccagga tggtgtgctt cagcaagcgg tcacgcgtgg cgacggcttg
      541 gtgggtgagg atgtcacgat gaatgtgcgc acaattaaaa atgttcccct gcgactacgc
      601 ggtaatcaga aattcgcagc attcttgcgc ggtcgtactg aaatacgtgg tgagattgta
      661 atgtacaagc aagatttcgc aaaactgaac gaacagcgta aagaggcagg cgagtcactt
      721 ttcaaaaacc cgcgtaatct tgcggcagga acaattcgtc agctcgatcc acagttagtt
      781 gcggcacgcc cattacactt tgtcgggtat gatattattc gcgatgattg ggacgatgtg
      841 ccaacaatcg catacggata ttctgcgatg tatgagcttg gtattgaaac cagcaaacag
      901 acgcaggtag tacagggcaa agcggcaatt attgattatg ttacacacct cggtgaaatt
      961 cgtggagact taaaatttaa cacagatggt gccgttataa agttaaatga tcgagcacaa
     1021 tttgaagatc ttggcattgt tggcaaaacc cctcgtgctg cagttgcctt taaattcgcc
     1081 gccgaagaag caacgacaat cgtaaaggat atcgtcattt cgattggccg cactggtgct
     1141 gcaaccccag tggctgtatt tgaccctgta caagttgccg gtacgacggt acagcacgct
     1201 agtctccata atgcagatga aattgctcgg cttgacgtgc gtcgtggtga taccgttatc
     1261 atctttaaag caggcgacat cattccacag gttcagagtg ttgttcccga acttcgcccg
     1321 aaagattctg ccgcaattag ttacgaagaa gaactaaaac gccaataccc tgagcttgaa
     1381 tttgtccgcc cagagggcga agtcgtctac cgcgtgaagg gactcactgg cgaccttata
     1441 ctgaagcgct cgctcgagca ttttgtttca aagggtgcat tggatattaa cacatttggt
     1501 gagaaaaacg ttgttgccgt aattgacgca gggtatgtga ataaccttgc ggatatctat
     1561 cgcctaacaa aagaaaaact attgcaactc gaccgtttcg ctgacatttc agctgcaaaa
     1621 ctcattgacg cagtcgccgc tgcaaaaaat ccaccgctcg aacgttttgt atatggactt
     1681 ggcattcgcc acgttggcgt gcagacagca attgatctag taagtcattt tggcagcctt
     1741 gatgcgatca ctcatgcgac gattgatgag ctcgaaggta tcgatggtat cggtaaagtt
     1801 gtagcggaga gtattgcggc atggtttgca gacgaagata atctcatgct tcttgatgag
     1861 tttacaaaac ttggcgtcgc gcctcatttt gagaaaaaga caggcaagtt gcttgggcaa
     1921 aattttgtcg ttacgggtac acttgaaagc atgggtcgtg acgaagcggc tgatgcaatc
     1981 cgcgcgctcg gtggtacatt ccaaacggct gttgctaaag atacgacgta tcttgtagcc
     2041 ggtggtaaaa ttggcgcaag caagttaaag aaggccgagc agtatggtac gaagatcatt
     2101 accgaggccg cactcctaga acttataaaa aataaataac acatccctga ggaggcagca
     2161 tgccaacatt tcaatac
//
LOCUS       DATIIX010000012        10868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_475866, whole genome shotgun sequence.
ACCESSION   DATIIX010000012 DATIIX010000000
VERSION     DATIIX010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 10868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10868
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475866"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VLF64_00285"
     CDS             complement(<1..649)
                     /locus_tag="VLF64_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HSW90416.1"
                     /translation="MNRKVRIARVVSLLGPAFVAAVAYVDPGNFAANFSAGAKHGYLL
                     LWVLIFANLMAGLVQYLSAKVGVVTGKSLPQVIASRLGDKSRIAYWVQAEVVAAACDL
                     AEVVGGAIALQLLFNVPLVAGGIITGLVSLLLLATYTRKGQHVYERVIVSLLLVIPIG
                     FVVGLFMHPPQVGQMLTGIVPRLEGKETILLAVAMLGATIMPHVVYLHSALSRDKH"
     gene            complement(639..1241)
                     /locus_tag="VLF64_00290"
     CDS             complement(639..1241)
                     /locus_tag="VLF64_00290"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HSW90417.1"
                     /translation="MKTEIEVKFCDIDIDATREILARTGATCEQPMRLMRRVIIETDQ
                     LAEHNAFVRVRDEGDRTTLAYKQFDEVSLTGAKEIEVTVSDFDATVALLEQVDLIHKS
                     FQESRRETWSFGDVEVVIDEWPHLNMYVEIEGSSEENVKQTAEKLGFDWNDAVFGPVT
                     EVYQHQYPDGSSRELVNIPRIAFDEPLPVIISGIGASHES"
     gene            complement(1313..2305)
                     /locus_tag="VLF64_00295"
     CDS             complement(1313..2305)
                     /locus_tag="VLF64_00295"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HSW90418.1"
                     /translation="MAGILGAGVQDGLLLKPIHYQWAKDLYDQAVANTWFPNEIQLAQ
                     DLADWKKLSDEEKHAFTFLVSYFNPNELLVNKALAFGVYPYVNAAECHLYLAKQMWEE
                     ANHCMSFEYILETFPIDREKAYEAHVEVPSMARKEEFETKYIKRMTEDTLDINTTEGK
                     KDFIRNLVAYNIILEGVWFYSGFMVALSFRQRNLLRNFGSLMDWIIRDESLHLKFGMN
                     LILTTLEENEDLQDPEFAEEIKQMILDAVEMEMEYNNDLLPKGILGLNADYVNQYVKY
                     LTDRRFEELGFGTHYNVDNPAKWMAAANDTLELVNFFESTNTSYEVNAGGKKKD"
     gene            complement(2315..2488)
                     /locus_tag="VLF64_00300"
     CDS             complement(2315..2488)
                     /locus_tag="VLF64_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90419.1"
                     /translation="MNASTIINDNMSLEEKLQAISDAMEQAQATANEEAAARGEVAAP
                     IDPADLTQCEGCQ"
     gene            complement(2505..4901)
                     /locus_tag="VLF64_00305"
     CDS             complement(2505..4901)
                     /locus_tag="VLF64_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HSW90420.1"
                     /translation="MAGIQVVKRDGTREDFDANKINLALVAASEGLPNQIQKVVEVAT
                     QLQLTLFDGITTEQLDESVIHTALQNVKDDPDFDKIAARLLLKTIYKKILGDYENAAE
                     LKKLHAQKFPAYIKAAAKDGLLDERMTDGRFDLKRLGSVLEPVHDELFKYIGVVTNRN
                     RYALRKNNGDPMETPQFTHMRIAMGLSYNEEDPTASAIDFYGHMARLEYVAGGSTRVN
                     AGGAFPQLSNCFLMEVQDDMESIAKSVRDTMWIAKGTGGIGITMTKLRAAGSPVHTTN
                     TESTGPIPFMKMVDTALFAVSRKGKKAGAAAMYMENWHLNFPEFLDLRQNSGDPYFRT
                     RFLDTAVYISDEFMKRVEKDQEWYMFDPAETPDLPELYGEAFSTRYKEYVKMADAGKL
                     RIFKTIPARQQFKAILTQLQAGSHPWVTWKDTINTRALNNNTGTIHCSNLCTEITLPQ
                     DEKNTSVCNLISINLSRHLTLEKTWEWDKLEQSVRSAVRQLDNLCDITNTPIPEAMNS
                     NLQNRAIGLGMMGFTDVIEKLGFSYESDQAYDLIDKLTEFISYHAIDESADLAIIRGS
                     YPQYKGSGWSKGYLPIDTLDQLAADRKVKVKVNRKAYLDWELLRKKVKKGMRNATLMA
                     IAPTANIGLVAGTTPGLDPQFAQIFSRSTLNGKFLEVNTNLVNDLRDLGIWEEVKDEV
                     LANLGDIQTVEAVPQRIKDVYKTSFQLSPYAFIEVAARAQKWVDQAISRNMYLETRDI
                     DEYVEIYTEAWRRGLKTTYYLHVKPRHQAEQSTTKVNKSEDIAARTGAKKVGFGFAKS
                     "
     gene            5261..5995
                     /locus_tag="VLF64_00310"
     CDS             5261..5995
                     /locus_tag="VLF64_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSW90421.1"
                     /translation="MENIITIKDFKMRFGNTEVIHGLTFNVKRGETFGFLGSNGSGKT
                     TTIRALLGIYQPTDGELLLDGKPYSVSGGMKLGYLPEERGLYKKESVIDAMVYFGELK
                     GMSRDEAKEASLAYLNRVNLANKANVRLDKLSGGQQQKIQLGITIMDNPELLILDEPT
                     KGFDPVNRKLLMDIIKEHRAKGATIMMVTHQMDEVERLCDHIVLLKDGKSYAYGTVEE
                     VKKKFNKKSLDEVFLQVYGDENEETR"
     gene            5992..7203
                     /locus_tag="VLF64_00315"
     CDS             5992..7203
                     /locus_tag="VLF64_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSW90422.1"
                     /translation="MMHNLSTVIRFEIVRTLKKKSYWIMALGFPVMMIAIFAIIYFSN
                     QSTNKAAKELQNQSFSIEVTDETHLIKPSILEALKAKTISSKQQGVDDVTSGKIDGYI
                     YYPSDISKHPIEAYGQDVGIFNNSRYDAVAGMILKQSIQNSVDVNVRSVISNQASTNV
                     TTYRDGKEYDPIKQMILPGLFLVLFYMLIAFSANQMLTSTIEEKENRVIEMILTTIEA
                     RTLVIGKIVSQVALSLLQGCIIVLPALAGYFLFHDKLNLPFIDLTTLPVDIPRILTGA
                     LVFTVSFLFFTGILVLIGSSVPTAKEASQFLGIVMIAIFGPLYAASLFISTPDAPLVR
                     FLSLFPFTAPIPLLLRNAVGNLHPWELAVALPILIVSATVILVLAVRVFRYGALEYSR
                     KVSLREIFGRR"
     gene            complement(7249..7336)
                     /locus_tag="VLF64_00320"
     tRNA            complement(7249..7336)
                     /locus_tag="VLF64_00320"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7300..7302),aa:Ser,seq:gga)
     gene            7452..8276
                     /locus_tag="VLF64_00325"
     CDS             7452..8276
                     /locus_tag="VLF64_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSW90423.1"
                     /translation="MPKATTSTPVIQLTTLTKRYGFGETSHKALDEVTLTIEKGEFIA
                     VMGPSGCGKTTLLNVIGLLDRASEGDYDLDGKSVASLSGQQQARVRSSQIGIVFQSFN
                     LIHRLNVLDNVALPLTYSGIPHLKRLERASEILKTFHLQEREYYMPWQLSGGQMQRVA
                     IARALVNHPSIILADEPTGNLDSRSSHVIMEELSQLHKQGNTIIMVTHNPNLTSYASR
                     VINMLDGKIASDTSTMPDPTAPKKSVKIPLNQHTKRKEKITKTTKTPRKKTRKAAK"
     gene            8273..9502
                     /locus_tag="VLF64_00330"
     CDS             8273..9502
                     /locus_tag="VLF64_00330"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSW90424.1"
                     /translation="MIQLLTDHVHNAYHSLRSNRGRTLLTVLGIAIGIASVTCTLAIS
                     DGVRKMVDTQVSAFNGRLAVVRPGFKSHDPNQLVSPVTQQSFSTSTLTDADTAALSKL
                     PNVEAAVPLMTIEGTIHAQHATGSNSVVLATTPDFAKVSNITMLSGKFLDDTTDDNTA
                     VIGLQLAISLFDTDHPIGEAFTMRGQQFTVVGVLRQDSAPINFNNVDLNNAVVVSFEQ
                     GKNFNQGRSQIQQIDLLTKKSSDLSDATKQAKSKLLSQHLGEEDFSITSGKAITQPTG
                     QLFQAMTGVMTAIAAISLIVGGIGIMNIMLVGVAERTREIGIRKAVGASQRAIVAQFL
                     IESLIMSGFGGLIGYGFGYLSAFIVSIFLYFTPAFSLQTAGIALGMMIAVGLFFGLYP
                     ALKAARKDTIESLRQYH"
     gene            complement(9553..10593)
                     /locus_tag="VLF64_00335"
     CDS             complement(9553..10593)
                     /locus_tag="VLF64_00335"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HSW90425.1"
                     /translation="MLDDINVLKQRDRSGALAVVADIYRQTALEVNLVDSEHDDRVIN
                     NIVVAGMGGSALAAEMAKVILHDSLTVPFEIVRGYELPNYTHENTLVIASSFSGSTEE
                     TVACFEAAIKKSCQVVAITSGGKLKDLATNNHVMLGLIPEESEARYGMIYNLRILLRV
                     LVEFGLASKNVYDEMTQCEPWLHQESELWDKDVPIEHNYAKQLALLAVGKTPVFYAGP
                     LMAPVAYKWKISWNENAKNVSVSNILPEFSHNEFAGWTSHPIEKPFAVFDLVSELENP
                     RILKRFNISDRLLSGRRPKATVVPIAGKGVMEQLLWGCILGEFVSVYVAILNNVDPTG
                     LEIIDKLKKELG"
     gene            10681..>10868
                     /locus_tag="VLF64_00340"
     CDS             10681..>10868
                     /locus_tag="VLF64_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90426.1"
                     /translation="MDIQPQSTQPISDDQELAKVLAGVNTDASEAETMPTAAAVQSPV
                     APMEPPVTMSAPDPTMPAV"
BASE COUNT         2967 a         2759 c         2221 g         2921 t
ORIGIN      
        1 cgtgcttatc gcggctaaga gccgagtgaa gatataccac atgtggcata atcgttgcac
       61 caagcatcgc gacagcaagc agaatcgttt ctttgccttc aagccgcgga acgatacctg
      121 tcagcatttg ccccacttgc ggcggatgca taaagagccc gacaacaaac ccaatcggaa
      181 taacaagaag taggcttaca atcacacgtt catacacatg ctggcctttt cgagtatagg
      241 ttgcgaggag tagtagtgaa acaagaccag taataatacc gcctgcaaca agaggtacat
      301 taaatagcag ttgtaacgca atagcaccgc ccactacttc tgcgaggtca cacgccgcag
      361 ccactacctc agcttgtacc cagtaggcaa tacgagattt atcaccaaga cgcgatgcaa
      421 ttacctgcgg taagcttttg ccagtaacca cgcctacttt cgccgataaa tactgcacta
      481 aaccagccat aagatttgcg aatataagca cccagagtag caggtaccca tgcttcgcac
      541 cagcgctaaa gttagcggca aaattacccg ggtcaacgta agctacggct gcaacaaatg
      601 ccggaccaag tagtgaaaca acccgtgcaa tacgtacttt acgattcatg cgacgcccct
      661 ataccactaa ttatcacagg aagtggctca tcaaatgcaa tacgcggtat attaacaagc
      721 tcacgtgagc taccatcagg gtattgatgt tgatacactt cagtcactgg accgaaaact
      781 gcatcattcc aatcaaagcc aagcttctca gctgtctgtt taacattctc ttcagacgaa
      841 ccttcaatct caacatacat attaagatgt ggccactcat caatgacaac ttctacgtca
      901 ccaaaactcc atgtctcgcg acgcgattct tggaacgatt tatgaataag atctacttgt
      961 tccaacagcg caacagtggc atcaaaatca ctgaccgtca cctcaatttc tttggcgccc
     1021 gtcaatgaaa cctcatcgaa ttgcttatac gctagcgtcg tacgatcacc ctcatctcga
     1081 acccgaacaa acgcattatg ctcggcaagc tgatcagtct cgataataac tcgccgcatg
     1141 agccgcatcg gctgttcgca agttgcacca gtacgcgcta atatctctcg cgtcgcgtcg
     1201 atatcaatat cgcaaaattt cacttctatc tcagtcttca tatatccctt ggctacaaaa
     1261 cgggcgtggt attcgttaaa acgccacgcc cgtcatttgc taaattacct tactaatctt
     1321 tcttcttgcc gccagcgttc acttcgtagc tggtgttagt gctttcgaag aagttcacaa
     1381 gctcgagtgt gtcattagct gccgccatcc acttcgccgg gttatcaaca ttgtagtgcg
     1441 taccgaaacc aagctcttca aagcgacgat cggtgagata tttcacatac tgattaacgt
     1501 aatcagcgtt aaggccaagg attcccttgg ggagaaggtc gttgttgtat tccatctcca
     1561 tctcaacagc gtccaagatc atttgcttaa tctcttcggc aaattctgga tcctgaaggt
     1621 cttcgttctc ttcaagcgta gtaagaatca aattcatacc aaacttcaag tgaagactct
     1681 cgtcgcggat gatccagtcc atcaggctac caaagttacg cagcaagtta cgctgacgga
     1741 aactaagtgc taccataaag ccagagtaga accacacacc ttcaagaata atattgtaag
     1801 caacgaggtt acgaatgaaa tcttttttgc cttcagtagt attaatatca agcgtgtctt
     1861 cagtcatacg cttgatatac ttcgtttcga attcctcctt gcgcgccatt gatggaactt
     1921 cgacatgtgc ttcgtatgcc ttctcacggt caattgggaa tgtttcaaga atatactcaa
     1981 aactcataca gtggttggcc tcttcccaca tctgctttgc aagatacaag tgacattctg
     2041 cggcattcac gtatgggtaa acaccgaatg caagcgcctt gttcacgagt aactcatttg
     2101 gattgaagta gcttacgagg aaagtaaatg cgtgcttttc ttcgtcactc agtttcttcc
     2161 aatctgcaag atcctgcgcg agctggattt catttggaaa ccatgtatta gcaactgcct
     2221 ggtcatataa atcttttgcc cattgataat ggattggttt aagcagtagc ccatcctgga
     2281 cacctgcacc taaaattcct gccatatttt cctcctactg acatccttcg cactgcgtta
     2341 aatctgctgg atcaattgga gctgcgactt caccgcgcgc tgcagcctct tcattagcgg
     2401 ttgcctgtgc ttgttccatt gcgtccgaga tagcctgcag tttctcttcg agtgacatgt
     2461 tgtcattgat aattgttgat gcgttcattt ttcatcccct ccttttatga ctttgcgaat
     2521 ccgaacccga cttttttagc accagtgcga gctgcgatat cttcagattt attcacctta
     2581 gtagtcgact gctctgcctg gtgacgtggc ttcacgtgta aatagtatgt tgtcttaagt
     2641 ccacggcgcc atgcttctgt gtagatttca acatattcat caatatcacg ggtttcaaga
     2701 tacatattgc ggctaattgc ctggtctacc catttctgtg cacgtgctgc tacttcgata
     2761 aatgcgtatg gactaagctg gaagctcgtc ttgtatacat cttttattcg ctgcggaact
     2821 gcctcaacag tctggatatc accaagatta gcaagaactt catcttttac ctcttcccag
     2881 atgccaagat cacgaaggtc gttcacgaga tttgtgttca cctcaaggaa cttaccgttc
     2941 aaggtgctgc gtgagaaaat ctgagcaaac tgcgggtcaa gacctggagt tgtaccagca
     3001 acaagaccga tgttcgcagt tggtgcaatc gccataagcg tcgcgttacg catacccttc
     3061 ttaaccttct tacggagtaa ttcccagtcg aggtacgcct tgcggtttac ttttactttt
     3121 acctttcggt cggctgcaag ctggtcaagc gtgtcaattg gcaagtaacc cttgctccaa
     3181 ccgcttccct tgtactgcgg atagctccca cgaattatgg caagatctgc tgattcatca
     3241 atagcgtggt aactaataaa ctcagtaagc ttgtcgatga gatcatatgc ctgatcgctc
     3301 tcatagctaa agccaagctt ttcaatcaca tcggtgaagc ccatcatccc aagtccaatt
     3361 gcacggttct gaaggttcga gttcatcgcc tctgggattg gcgtgttagt gatgtcacag
     3421 agattatcaa gttggcgaac agcactgcgc acggactgtt caagcttatc ccactcccat
     3481 gtcttttcaa gggtcaggtg gcgactcaaa ttaatactaa taaggttaca gacagaggta
     3541 ttcttctcgt cctgaggcag tgtaatctca gtacaaagat ttgaacagtg aatagtaccg
     3601 gtgttgttgt ttagtgcacg agtgttaata gtgtccttcc atgttaccca tgggtgacta
     3661 cccgcctgaa gctgtgtaag aattgccttg aactgctgac gtgccgggat agtcttaaat
     3721 atccgcagct tgcctgcatc ggccatcttg acgtactctt tgtatcgagt actgaacgct
     3781 tcaccgtaaa gttcaggtag gtctggcgtc tcagccggat caaacatgta ccattcttgg
     3841 tctttttcaa cacgcttcat gaattcgtca gaaatatata cagccgtatc aaggaaccga
     3901 gtacgaaagt atgggtcacc agagttctga cgaaggtcga gaaattccgg gaaattcagg
     3961 tgccaattct ccatgtacat tgctgctgcc cctgcttttt tacctttacg gctcacggcg
     4021 aagagtgcgg tgtcgaccat tttcataaat ggaataggac cagtgctttc agtattggta
     4081 gtgtgcacag ggctaccagc cgcacgaagc ttagtcatcg taataccaat cccgccggtg
     4141 ccttttgcaa tccacatggt gtcacgtaca cttttcgcga ttgactccat gtcgtcttgc
     4201 acttccataa gaaaacagtt cgaaagttgt gggaacgcac cccctgcgtt cacacgagtc
     4261 gaaccacccg ccacgtattc gagccgcgcc atatgcccgt aaaaatcaat tgccgaagcg
     4321 gtcggatctt cctcattata gctaagaccc attgcgatac gcatatgagt aaactgtggc
     4381 gtttccatcg ggtcaccatt atttttgcga agcgcatagc ggttgcggtt ggtcacaaca
     4441 ccaatatatt tgaagagctc gtcatgtact ggctcaagca cgctcccaag tctctttaga
     4501 tcaaaacgac cgtcagtcat gcgctcatca agtaaaccat ccttagccgc agctttaata
     4561 tatgcaggaa acttctgggc atgcagtttc ttaagctcag ctgcattctc gtagtcgccc
     4621 aaaattttct tatagatggt cttaagaaga agccttgcag caattttgtc aaaatcagga
     4681 tcatccttaa cgttttgtaa tgccgtatgg atgacagatt catcaagttg ttctgtggtg
     4741 atcccatcaa ataatgttaa ctgaagttgt gttgcaactt ctactacttt ctggatttgg
     4801 tttggaagcc cctcactcgc cgctacaaga gctaaattaa tcttattagc gtcgaaatct
     4861 tcacgggtgc catcgcgttt tacgacttga ataccggcca tattctattc ctttgttata
     4921 gttattacct tcaccgaccc cagcccctca ggggcgcaag aaagccacct actagtgtag
     4981 tgtgctagtt ttacgacaaa cttgcataag tgtctatttt gctgaactac tacatatgta
     5041 gcgtctacta ctcatgataa gcgctatata tagcgatttc aagcgtttta acttgttttt
     5101 ttaacaataa acctaaataa caccttatag cgtagatggt aatttttact acgctatatg
     5161 tagcgtatga ttcacacatt gattcatttg aaataccgac ggacttgtaa gagtagagcc
     5221 tcttaagcgt aaccgtagaa ggtgtataat aataaacaat atggaaaata taatcactat
     5281 aaaagacttt aagatgcgat tcggcaatac cgaagtcatt catggcctta cctttaacgt
     5341 aaagcgcggc gaaacattcg ggtttcttgg cagcaacggc tccggtaaaa ctaccactat
     5401 ccgtgcatta ctaggcattt atcagcctac tgacggtgag ctacttctgg acggcaagcc
     5461 atacagcgta tccggcggta tgaagctcgg gtacctacct gaagagcgtg gcctctacaa
     5521 aaaagagtct gttattgacg caatggtata ttttggcgag ctaaaaggaa tgagccgtga
     5581 tgaagccaaa gaagcctcac tcgcctacct taaccgtgta aatcttgcca acaaagcaaa
     5641 tgtgcgcctt gataaacttt ctggtggcca gcagcaaaaa atccagcttg gcattaccat
     5701 tatggacaac cccgagctcc tcatacttga cgaaccgacc aagggattcg acccagtcaa
     5761 ccgcaaactc ctcatggaca tcattaaaga gcatcgtgca aaaggcgcta ctatcatgat
     5821 ggtaacccac caaatggatg aagttgagcg cctctgcgat cacatcgttc tattgaaaga
     5881 tggcaagtca tacgcctacg gcaccgttga agaagtaaag aagaagttca acaaaaagtc
     5941 actcgacgaa gtatttctcc aggtatatgg cgatgaaaac gaggagacaa gatgatgcac
     6001 aatctttcaa ccgttattcg attcgaaatt gttcgtactt taaaaaagaa atcatattgg
     6061 attatggcgc ttggcttccc agtaatgatg atagcaatat ttgcaatcat atatttctcg
     6121 aaccaatcaa ccaacaaggc tgctaaggag cttcaaaacc agtcattcag cattgaagtt
     6181 accgatgaga cacatttgat taagccttct attcttgaag cactgaaagc aaaaaccatc
     6241 agcagcaagc agcaaggtgt cgatgatgta acttctggta agatagatgg ctatatttac
     6301 tatccaagtg acatcagtaa acatccaatt gaagcctatg gtcaggacgt tggcatcttt
     6361 aataacagtc gctatgatgc agttgcaggt atgattctca aacaatctat ccaaaattca
     6421 gtcgatgtga atgtgcgcag tgttataagc aatcaagctt caacaaacgt cactacgtat
     6481 cgcgatggca aagagtatga tcctatcaag caaatgatcc tgcctgggct attcttagtg
     6541 ctcttctata tgttgattgc attctccgct aatcaaatgc tcacgagcac catcgaagaa
     6601 aaagaaaatc gcgtgatcga gatgatcctc accaccatcg aggctcgcac gttggtaatt
     6661 ggcaaaatcg tatcacaggt tgcgctttcc ctactccagg gctgtatcat cgtgcttcca
     6721 gcacttgcag ggtatttctt gttccacgat aaactcaatt tgccgtttat tgatctaacg
     6781 acacttccgg ttgatattcc gcgcatctta accggcgcgc ttgtctttac cgtgagcttc
     6841 ctgttcttca cgggtatact agtattgatc ggttcgagcg taccaaccgc caaagaagca
     6901 agtcaattcc ttggcatcgt tatgatcgca atctttggcc cactctacgc cgcatcgcta
     6961 ttcatctcaa caccagatgc accgcttgtt cgattcttga gtctattccc attcacggcg
     7021 ccaattccgc tcttactccg caatgcagtt ggcaatctgc acccatggga gctcgctgtc
     7081 gcgctaccaa ttcttattgt gtctgcgacc gttatattag tactcgccgt ccgcgtattc
     7141 cgctacggtg cgctcgaata tagccgcaaa gtatcgctcc gcgaaatttt tggtcgacgc
     7201 taagtctggc cataaaaata cgccgaccca ttggtcggcg tatttttatg gcggagagac
     7261 agggattcga accctgggta ctttgcagta cacacgcgtt ccaggcgtgc caattcaacc
     7321 actcttgcac ctctccgtag tattcacatt atagcaaagt cattcccctt gtgtttttta
     7381 ttgtaaggtt gtattttata cacttctcat ctgttgcttt cccgagctct gagtgcctat
     7441 aataagagaa gatgccgaag gccaccacat ccacaccggt tatacaactt accactctca
     7501 ccaaacgcta tgggttcgga gagacctctc ataaagcgct tgacgaagta acgctcacaa
     7561 tcgaaaaggg cgaatttatt gccgtcatgg gcccatctgg ctgtggtaaa acaacgctct
     7621 taaacgttat cggtctactt gatcgtgcta gcgagggtga ttatgatctt gatggcaaat
     7681 ctgtcgccag cctttcgggg cagcaacaag cacgtgttcg ctcaagccaa attggcatcg
     7741 tgttccaaag cttcaatctt attcatcgcc taaacgtact cgataatgtt gcgctaccac
     7801 ttacctacag cggcattcct catctcaaac gactcgagcg cgccagcgaa attctcaaaa
     7861 cattccactt acaggagcgt gaatactata tgccatggca actaagcggc ggccaaatgc
     7921 agcgcgtagc catcgcccgc gcactcgtaa accacccgag tatcattttg gctgacgagc
     7981 ctacgggcaa tctcgatagc cgttccagcc atgtaattat ggaagagctc agccagcttc
     8041 ataagcaagg caacacaatt attatggtga cgcacaatcc taacctcaca agctatgcaa
     8101 gccgtgtcat caacatgctc gatggcaaaa tcgcgagcga caccagcacg atgccggacc
     8161 cgacagcgcc taaaaaatcc gtaaagattc cactgaatca acacacaaaa cgtaaagaaa
     8221 agatcaccaa aacaacaaaa acaccacgta aaaagacacg aaaggcagcg aaatgatcca
     8281 gttacttacc gatcatgttc ataatgccta ccactcatta aggagtaatc gtggacgcac
     8341 attacttacc gtactaggta ttgcaatcgg catcgcaagc gtaacatgca cattggccat
     8401 tagtgatggc gtacgaaaaa tggttgatac gcaagtttcc gcattcaatg gcaggcttgc
     8461 cgttgttcgc cctgggttta aatcacatga tcctaatcaa cttgtaagcc cggtcacgca
     8521 gcagtcattc agcacaagta cgcttaccga tgcggatacg gctgctctat caaaattacc
     8581 gaatgttgaa gctgcggtac cacttatgac aatcgaaggc acgatccatg ctcaacacgc
     8641 gaccggctcc aacagtgttg tgctagcaac aacgcctgat tttgccaagg tatcgaacat
     8701 tacgatgctc tcaggaaaat tcctcgatga cacaacagac gacaataccg ctgtaattgg
     8761 tcttcagctc gctatatcat tatttgatac cgatcatccc atcggcgaag cattcactat
     8821 gcgcggccag cagtttaccg ttgtcggtgt cctccgccag gatagtgcac cgattaactt
     8881 caataatgtt gacctcaata atgccgtcgt cgtaagcttc gagcaaggaa agaactttaa
     8941 tcaaggccgc tcacagattc agcaaattga tttacttacc aaaaaatcat cagatttatc
     9001 tgatgccacc aagcaagcaa aatcaaaact actttcacaa cacttaggcg aagaggattt
     9061 ctccattaca agcggcaaag ccatcaccca gccaaccggt cagctgttcc aggcgatgac
     9121 aggcgtcatg actgcaatcg ctgctatatc gcttatcgtg ggtggcatcg gcattatgaa
     9181 tattatgctc gtcggtgtcg ccgagcgaac gcgagaaatc ggtattcgca aggccgttgg
     9241 tgcgagccaa cgtgcaatcg ttgcccagtt ccttattgaa tcactcatta tgagtggatt
     9301 tggcggatta atcggctatg gatttggcta cttatcggct tttatcgtca gtatcttcct
     9361 ttatttcacc ccggcattta gcttgcaaac agctggcatc gcacttggga tgatgattgc
     9421 cgttggccta ttctttggcc tctaccccgc tctaaaagca gcacgaaaag acactatcga
     9481 atcactacgg caatatcatt aaataaatga aaactgactt taataataag tcgacccgcc
     9541 gccccgcttc tttcatccaa gctctttttt aagcttatca ataatctcaa gcccagttgg
     9601 atcaacattg ttcaagattg ctacgtacac actgacaaac tcacccaaaa tacagcccca
     9661 aagcagctgt tccatcacgc ccttaccggc aataggcacg accgttgctt ttggtcggcg
     9721 gcccgaaagt aaccgatcac tgatattaaa tcgcttcaat atacgaggat tttcaagctc
     9781 gcttaccaaa tcaaatacag caaatggttt ttcaatcgga tggctcgtcc aacccgcaaa
     9841 ctcgttatga ctgaattccg gtagtatgtt cgaaaccgac acattctttg cattttcatt
     9901 ccagctgatc ttccacttgt atgccactgg tgccatgagc ggtccggcgt aaaataccgg
     9961 tgtcttccct accgcaagta atgccaactg ctttgcatag ttatgctcaa tgggtacatc
    10021 tttgtcccat aattcgctct cttgatgtaa ccatggctcg cattgcgtca tttcatcgta
    10081 tacatttttt gatgcaagcc caaactcaac aagtacgcgt agcaagatgc gaagattata
    10141 aatcatccca taacgtgctt ccgattcctc aggaatcaac ccaagcatta cgtgattatt
    10201 cgttgcgagg tctttcagtt tgcctccgct cgtaatcgcg accacttggc aagatttttt
    10261 aattgctgct tcaaaacaag ctacagtctc ctcggtacta cccgaaaagc tacttgctat
    10321 cacgagtgtg ttttcatgcg tataattcgg cagctcatat ccacgaacaa tttcaaatgg
    10381 cacagtaagg ctatcatgca aaatcacctt tgccatttca gccgcgagcg cagaaccgcc
    10441 catacctgca acaactatat tattgattac tcgatcatca tgttcactat caaccagatt
    10501 cacctcaagt gctgtctggc gatatatatc cgccaccacg gctagcgccc ctgatcgatc
    10561 gcgctgcttc aatacgttta tgtcatccaa catcgtcacc actccccttg cgaaatattc
    10621 tcttactatg atacagtatt gtataagtaa gcataactac taaatattca agaggtgggc
    10681 atggatattc aaccgcaatc aacacaacca attagtgacg accaagaact tgcgaaagtt
    10741 ctcgctggag taaacacgga cgcaagcgag gctgaaacga tgcctacagc tgctgcggtg
    10801 cagtcaccag tagctccaat ggaacctcca gttacgatgt ctgcacccga tcctactatg
    10861 ccagctgt
//
LOCUS       DATIIX010000013        10046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_225732, whole genome shotgun sequence.
ACCESSION   DATIIX010000013 DATIIX010000000
VERSION     DATIIX010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 10046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10046
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225732"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VLF64_00345"
     CDS             <1..185
                     /locus_tag="VLF64_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HSW90427.1"
                     /translation="LSHYRYLEHGAHWAILVLGIIMLAKLYHIEPAEWFTGSIGLIFI
                     ATAVASSIIERRKAAT"
     gene            complement(178..1170)
                     /locus_tag="VLF64_00350"
     CDS             complement(178..1170)
                     /locus_tag="VLF64_00350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSW90428.1"
                     /translation="MKHLKQDALRLTGTYLAIIMAMSVGFSVIFYSVSMRELDRRPHA
                     ISLRDIAPDRDTQLTFDDYLSNRANEARTSLLVDLVIVNLMALISGGILSYLLAQYTL
                     RPIEENMEAQSQFVSDASHELRTPLTALRTANEVALRDKKLKLADARTVIADNIEDIT
                     RLQSLADAMLGLLRDDSSGLQDNVSLPDIVSEAMSMVVSQAQAKNISVEDATGDTTVY
                     GNARSLTQLLTILLDNAIKYSPAGSAVHVASEAQGRSLVLSVKDEGIGMDEDTVRNAF
                     TRFYRAEESRSTTGYGLGLSIAKKITEAHHGKITIKSMPGEGSVFTVTLPLESM"
     gene            complement(1170..1844)
                     /locus_tag="VLF64_00355"
     CDS             complement(1170..1844)
                     /locus_tag="VLF64_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSW90429.1"
                     /translation="MKILVVDDEIRIARAIKQGLEQDGYAVDLAYDGEDGYNSARADE
                     YDCIILDVMMPEMNGYEVLRKLRDDGYKTPILMLTAKDQARDVVKGLDNGADDYLAKP
                     FSFDVLGARLRSLLRRPHDMVDNVLTAKDLEMDIVRREVRRQGKLVQVSSKEFAILEY
                     LLRNKNRVLSKTNIMTHVWDFDADILPNNVEVFINYLRGKIDKPFKGDQLIQTVRGFG
                     YVIRDE"
     gene            complement(1945..2559)
                     /locus_tag="VLF64_00360"
     CDS             complement(1945..2559)
                     /locus_tag="VLF64_00360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HSW90430.1"
                     /translation="MITPTLLKQFLIISDQVDARELTVILNELERLLARGRQGNIVEL
                     GCYVGTTSLFIRRLMNEYKVTDEFHVYDSFAGLPEKTPQDISVAGDQFVAGALYASRR
                     TLLETFKKSHLEPPIVHKGWFSDLVSEDMPDGILFAFLDGDYYESIRDSLKLVENKLL
                     PGAVIIVDDYASEALPGAAAAADEWASRKGHRVQSMASLGIIRT"
     gene            2611..2919
                     /locus_tag="VLF64_00365"
     CDS             2611..2919
                     /locus_tag="VLF64_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90431.1"
                     /translation="MDTNLHSLLNDTSGGTNNSLFSLPAGITTLITVFTITASVASIV
                     IVVLYFVNALTTYRAHRATIEMRDILREMNERDKARSKPVAASAMANPAIESELTAKN
                     "
     gene            complement(2916..3446)
                     /locus_tag="VLF64_00370"
     CDS             complement(2916..3446)
                     /locus_tag="VLF64_00370"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSW90432.1"
                     /translation="MRKSVRGFTIVELLIVVVVIAILAAITIVTYNGAQKRALNTARL
                     TEVIAWKKQLLLYYANNGQGVDVTAGNYCLGYGFPSGKCRNYTTTGSNTYNEADNAAL
                     MTELKGATGGSLPSGSRQPVGNYIGPYVNIWSGQSGFSLYSFFSGGSSNCPAPLTYVW
                     DDGAGDLMCGQDVSYN"
     gene            complement(3486..4550)
                     /locus_tag="VLF64_00375"
     CDS             complement(3486..4550)
                     /locus_tag="VLF64_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90433.1"
                     /translation="MNGRVTTEKLSFKLILLVIASIILAIMTFAINWRDVGQFWSYTN
                     VAKMILAGIIAYITVFWRTSAQQQYKLFKSVVTLAVPVFAIFATVTLLINPNLAEVLV
                     RESHAFENIEAWFLFTGAIFMFIATVRLMRAGRKLEAVGAAVMALAIFAIAGEEISWG
                     MWLFNEHTNTFFHQLNSQDETNLHNINTFLSEDLYYFGSFVLLVMVPFFKDSIARLLA
                     KFKLERLTSLLPSTWMMLPWTVMAGFLASYSYMQTTILLGFFGSVLVLIHALLSSPSK
                     TRAWMTVGFLALLLIVGFVFTFSWQSVYIDRIRGGSPKEYMEVMTSFGLCVYAVDVYV
                     RLFIHGLDERPLIKRVFTKS"
     gene            complement(4552..5559)
                     /locus_tag="VLF64_00380"
     CDS             complement(4552..5559)
                     /locus_tag="VLF64_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90434.1"
                     /translation="MQRIRINIKFLLVAAAMLVTPFMVIPQNAWAVTSANNQANTLKV
                     SPVRSDISVKPGESKTVEVTVTNLTKADISIQPYENDFIAKGEDGTPALILNDNQYAP
                     THSLKRFMLPLGGFTVPAGKAKAVKVTISVPPTAQPGGYFGAIRFAPASPADGGQVNL
                     NASVASLMLLTVQGPMTEKLNVTDYSVQQNGKSGTYFQNPNNLAFSFRFEDKGSVQEA
                     PFGNISVKNGDKVIYSHEFNNESPREVVLPDSARRWTVPLKDIGTFGYYTVEGTLTYG
                     SKNETVNISQSFWVIPFAVIIGGIVGLLVLIGLIVGIVAFLRSYKRRIINTQGRGGRR
                     R"
     gene            complement(5673..6830)
                     /locus_tag="VLF64_00385"
     CDS             complement(5673..6830)
                     /locus_tag="VLF64_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90435.1"
                     /translation="MSSFRSISQRTGRMATLAALVVATLGQAVLPVLVSADSITSRSI
                     ELSSATKSATGVSYKVAFTATKAAGAYVVEFCSNSPVIPDACTAPTGFSAASAALGAA
                     GTTATAVTGSTNKLVVTNVISAAESITDTFTGITNPSSSATMYARIVSYDNATDAGNY
                     ASATLGSGVQDQGSVAIAITDTIGVSGAVAESLLFCVSGSASGGGNPITSGCTGTLTA
                     PTLKLGEDNGTGGVALVPSAISSGTIYTQLSTNAANGVVVNLKSSAASCGGLKRVDDG
                     LCGIKPANGTNGNVAAGSALFGVKTGAAVSDTGNGSLAIASGYNATDYKLNAATDNST
                     GVTSTYGDEVFNAPGYANSLDMPLTFGASVANNTPAGNYSADLNLVATGTF"
     gene            complement(6947..7891)
                     /locus_tag="VLF64_00390"
     CDS             complement(6947..7891)
                     /locus_tag="VLF64_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HSW90436.1"
                     /translation="MMYAARTIQRWGTLSIVGATVVALNLLVQPANAITQIPNPEPKP
                     GSFGLAASKTQVPPTQGATISTPGSGSGFTNSPITVAGICPNGLLVQLYDNGVMVGSS
                     LCNGGSFSMQVSLFAGSNELAATVFDDLGQTGPTSAAVTITYSDTHFSAFGSLVTLTS
                     SYGRRSAPAGTSLTWPLQLSGGSGPYAFSIDWGDGSKPQLKSQAFTGVVPIDHPYSKA
                     GIYPVSVTATDADGVSAFLQVVAVSSGKVDSTTAGVVGGDSSGKASQAPPQVLWVPTI
                     TMFALFLPTFWLGRLSQMVSIRNKMLKERDTFENSKNR"
     gene            complement(7943..9007)
                     /locus_tag="VLF64_00395"
     CDS             complement(7943..9007)
                     /locus_tag="VLF64_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90437.1"
                     /translation="MKHIKSLVLAVFAVLLSVTYVLPPTQVAADSSSALGIAPKKNYV
                     IEPGNSVQDKLNITNLDQATALNLTLRVIDFSFTGNGGTPKLFLDTNTPQTTWSLRSY
                     LKVPQTVTIDPGKSKQVDLNVAIPKGHGAGSYYSAIVYSSGSGQGGNIGLNASGVTLV
                     FVNVPGKVKENLDFTHIGAYDRAAQGDLSGYHYFNTQAPDTIAYTLKNNGNVVEAPAG
                     NITLRDIFGRTTTINELNPSSSPALIGQERTFLTCVKLKPEDVKLGNTTTKSSTCDAS
                     GLWPGYYSVSADLFYGQNGNLTQEVTKVGGFWYLPWWFIGVAVVVLGVAGFYGWRAYN
                     KIRNKLYGPRVAKTKSARRK"
     gene            complement(9065..>10046)
                     /locus_tag="VLF64_00400"
     CDS             complement(9065..>10046)
                     /locus_tag="VLF64_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90438.1"
                     /translation="FMVPVSATTRFETRGLLMQTTDPGATTSYTVTLKFMSPEQVGSL
                     DMLFCNDPIPYMPCAIPTGLDVSHATLTNQSGETGFSIHSQTQNHIILARAPTVPIYP
                     SESSFTFSGIVNPTDTSQAFSIRMKSLSSIDGTGPLIDFGSVRGQVETGIMIQSQVPP
                     MLVFCAAQHVDDNCAGTDETYYRDMGTLSPHDTLTAQSQLAVGTNASQGFVVTANGPP
                     MSAGTSVINSPALPTESQQGTNQFGINLVENTVPTIGNNPEGTWANAVPSADYGIPNK
                     YKFVSGDVVAYSPNVSLMRKFTVSYILNSSKDLRAGVYTTTITLIASGRF"
BASE COUNT         2606 a         2437 c         2238 g         2765 t
ORIGIN      
        1 cgcttagcca ctaccgctac cttgagcacg gtgcacactg ggctatcttg gtgctaggta
       61 ttattatgct tgcgaagctc taccatattg agccagccga gtggttcact ggttcaatag
      121 ggcttatctt tattgccacg gccgttgcta gcagtattat tgaacgcaga aaagcagcta
      181 catagattct aacggaagtg tcacagtgaa gacggagcct tcacctggca tacttttgat
      241 agtaatctta ccgtgatgtg cttcggtgat tttcttagca atcgagaggc caagaccata
      301 acctgtggta cttcgtgatt cttcagcgcg atagaagcgc gtaaatgcat tcctaaccgt
      361 atcttcatcc atgccgatgc cctcatcttt tactgacagt accagcgaac ggccctgcgc
      421 ctcgcttgct acatgcaccg cgctacccgc gggactatat ttaattgcat tatcgagcag
      481 gatcgtaagg agctgcgtca ggctacgagc gttgccatat actgtcgtgt cgcccgttgc
      541 atcttcaaca gagatattct ttgcttgggc ctggctgacc accatgctca ttgcctcact
      601 tactatgtct ggtagtgaga cattgtcttg aagtccacta ctatcgtcgc gaagtaggcc
      661 aagcattgca tcggccaatg attgtagccg cgtgatatcc tcaatattgt cggcaatcac
      721 cgtacgggca tcggcaagtt ttagtttttt atcacggagc gcgacttcgt ttgctgtgcg
      781 cagtgctgtt agcggcgtgc gcagttcatg gcttgcatca ctcacaaact gtgactgtgc
      841 ctccatgttt tcttcgatcg gccgtagtgt atactgcgcc agcaggtagc ttaaaatacc
      901 ccccgatatt aacgccatga gattcacaat gactagatca acaagcaacg atgtgcgagc
      961 ctcattggca cggttagaca gataatcatc aaaagttaat tgtgtgtcgc gatcgggtgc
     1021 gatatccctc agtgagattg catgcggacg cctatcgagc tcgcgcatcg aaacactata
     1081 aaagataacg ctaaatccaa cactcattgc cataatgata gcaaggtaag tgccggtgag
     1141 tcgcaaggca tcttgtttaa ggtgtttcat tattcgtctc tgattacgta tccaaaccct
     1201 cgtaccgttt ggatgagttg gtcgcccttg aacggtttat caattttacc acgaagatag
     1261 ttgataaata cttcgacatt atttggcaag atgtcggcat caaagtccca gacatgcgtc
     1321 atgatattcg tcttggaaag cacacggttt ttgttgcgga gcaagtattc gagaattgca
     1381 aactccttac ttgatacctg tacaagttta ccttggcgac ggacttcacg gcggacaatg
     1441 tccatctcaa gatctttagc ggtcaacacg ttatctacca tgtcgtgtgg gcgacgcaat
     1501 agtgagcgga gacgtgcacc gagtacatcg aacgaaaatg gcttcgcaag ataatcatcg
     1561 gcgccgttat cgagaccttt cacgacatcg cgtgcttggt ctttcgcagt caacattaaa
     1621 atcggcgttt tgtagccgtc atcgcgaagc ttgcgcaata cctcgtagcc attcatctct
     1681 ggcatcatca catcaagaat aatacagtcg tattcgtcag cccgcgccga gttatagccg
     1741 tcttcgccat catatgcaag atcgactgcg tagccatctt gctcgagtcc ttgcttgatt
     1801 gctcgtgcga ttcgtatttc gtcgtcaaca actaaaatct tcatggttat agtatactcc
     1861 tgtcacttcg tagacgcggg cccttaatcg tatactacca gttttgcaaa tacttcttgg
     1921 caaatgctta aagaaaccca tttgctaagt gcggataatc cctaaacttg ccatactttg
     1981 aactcggtgc cctttacgac tcgcccattc atcggcagct gcggctgcac caggtagcgc
     2041 ctcacttgca taatcatcaa caataattac agcgcctggt agcaacttat tctcaactag
     2101 cttcagcgag tcgcgaattg actcgtaata gtcgccatct aggaaagcaa acagaatacc
     2161 atctggcatg tcctcagata ctaaatcgct gaaccagcct ttatgtacaa tcggcggctc
     2221 taggtgtgat tttttgaaag tttcaagcag cgtgcggcgc gaagcatata atgcgcctgc
     2281 tacaaactgg tcaccagcta cacttatatc ttggggagtt ttttctggga ggcccgcaaa
     2341 tgaatcatac acatgaaatt catcggttac tttatattcg ttcattaacc ggcggataaa
     2401 cagcgaggtt gtgccaacat aacaaccaag ctcgacaata ttaccctgcc tacctctggc
     2461 aagaagtcgc tcaagttcat tcaaaataac cgtgagctca cgcgcgtcga cttgatcgga
     2521 aatgattagg aattgcttga gtaatgtagg tgtgatcata ctgttgtcac cgattgtacc
     2581 ataacaatat gcttatgcta tgcttttagt atggatacga acttacactc attgcttaat
     2641 gacacctctg gcggtactaa taattcactg ttttcactac ctgccgggat taccacactc
     2701 attactgtgt tcactattac tgcaagcgtc gcctctattg ttatcgtggt gctttatttc
     2761 gttaacgcac tcaccactta ccgtgcacat cgtgccacta tagagatgcg tgacatctta
     2821 cgtgagatga atgaacgcga taaagctcga tcgaaaccag ttgcagcatc cgctatggca
     2881 aatcctgcta tagaatccga acttacggct aaaaattaat tataggatac atcttggccg
     2941 cacataaggt cacctgcgcc atcgtcccat acatatgtta atggtgccgg gcaatttgaa
     3001 gacccgccgc tgaagaagct atatagacta aatccagatt ggccactcca gatatttaca
     3061 tatgggccga tatagttccc tacaggctgt cgactcccag aaggcagtga tccccctgtg
     3121 gcacccttta gttctgtcat caatgctgca ttatctgctt cgttatacgt attagatccg
     3181 gtcgttgtat agtttcggca ttttccgctt ggaaacccat atcccaagca ataatttccc
     3241 gctgttacat ccaccccctg cccattattt gcgtaatata gcagcaactg ctttttccag
     3301 gcaatcacct ctgttagccg cgcggtattg agagcacgtt tttgtgcacc attatatgta
     3361 actattgtaa tagccgcaag aatagctatc acgacgacaa caatgagaag ctcgacaatt
     3421 gtgaaacctc ttacgctttt acgcatatct ttattgtacc ccacctaaac cgttttagtt
     3481 atccactacg actttgtaaa aactcgctta atcagaggtc tttcatccag tccatgaata
     3541 aatagtcgta cgtagacatc tacggcgtat acacagaggc caaatgaggt cattacttcc
     3601 atgtactcct ttggcgagcc accgcgaata cggtcgatat atactgattg ccagctaaat
     3661 gtgaagacga atccgactat aagcagtaag gctaagaacc ctactgtcat ccatgcacga
     3721 gttttcgatg ggcttgagag aagggcgtgg ataagcacaa gcaccgaccc aaagaatcca
     3781 agcagaatag tggtctgcat atagctgtat gacgctagga accctgccat tactgtccac
     3841 ggtagcatca tccaagtgct tggcaatagc gacgtaaggc gctctaattt gaacttagca
     3901 agcaggcgcg cgattgaatc tttaaagaac ggtaccatga cgagtaatac aaacgagcca
     3961 aagtagtaga gatcttcgct aaggaatgtg tttatattgt gcaagttggt ttcgtcctgt
     4021 gaattaagct ggtggaagaa tgtgtttgta tgctcgttaa atagccacat gccccatgat
     4081 atttcttcac cagcaatagc gaatatcgct aaagccatca ccgccgcgcc aaccgcttcg
     4141 agctttcggc cagcgcgcat gagtcggact gttgcaatga acataaaaat agcaccagta
     4201 aataagaacc atgcctcgat attttcaaac gcatgacttt cgcgcaccag tacctctgct
     4261 aggttcgggt ttatgagtaa tgtgacggtg gcaaagatag caaataccgg cactgctaga
     4321 gtaacgactg atttgaatag cttatactgt tgctgggcgg atgtgcgcca gaacactgtg
     4381 atatatgcaa taatccctgc caagatcatc ttggcgacat tggtatatga ccagaattgc
     4441 ccgacatctc gccaattaat cgcaaatgtc atgattgcta gaattattga agcaataacg
     4501 agcagtataa gtttgaatga aagtttctca gtggtaacac ggccgttcat attaacgcct
     4561 gcgtcccccg cgaccttggg tattgatgat tcggcgctta tagctacgaa ggaaggcaac
     4621 gatgccgaca atgaggccga ttagtacaag cagaccaaca ataccaccga taatcactgc
     4681 aaatggaatt acccagaatg attgagagat atttacggtt tcatttttgc taccgtaggt
     4741 aagtgtaccc tcaacggtat agtagccgaa tgtgccaata tcttttagtg gaactgtcca
     4801 tcgacgagcg ctgtcaggta gtaccacctc acgaggggat tcgttgttaa attcatgact
     4861 atagattact ttatcaccgt tcttcacaga aatattacca aatggagcct cctgcacgct
     4921 gcccttgtct tcaaaccgaa aactaaatgc aagattgttg ggattctgga aatacgtacc
     4981 gcttttacca ttttgctgaa cactgtagtc ggtgacgtta agcttttcgg tcattggacc
     5041 ttgcactgtt aagagcatca gcgatgcaac actggcgttt aagtttacct ggccaccatc
     5101 agctgggctt gcaggcgcaa agcgaatggc gccgaaatag cccccgggtt gcgcagtagg
     5161 gggtacagaa attgtaacct tcacagcctt tgctttacca gcagggactg tgaaaccccc
     5221 aagcggcaac ataaaacgct tcaagctatg cgtaggtgca tattggttat cgtttaaaat
     5281 aagtgcaggt gtgccatctt caccttttgc aatgaagtcg ttctcatatg gctgaattga
     5341 gatgtcagct ttagtgagat tagtcaccgt aacttcaact gtcttacttt cgcccggttt
     5401 tacagatatg tcgctccgaa caggtgatac tttaagcgta tttgcctgat tattggcact
     5461 tgttaccgcc catgcattct gcggaattac catgaacggt gtcacaagca ttgctgcggc
     5521 aactaataaa aacttgatgt ttattctgat gcgctgcata gccacctcag tatactcccc
     5581 cattacctaa acttacgttt agtataacag actgccacaa aaacaacacc ccttacgata
     5641 gaggggtgtt gtttttgtta ttctagaatt gtttagaaag taccagttgc aactaagttg
     5701 aggtcagcag aataattacc tgcaggtgtg ttattagcta cactcgcccc aaaggttagg
     5761 ggcatatcca ggctgtttgc atagccagga gcgttaaata cctcgtcccc gtatgtactc
     5821 gttactccag ttgaattatc tgtcgccgca ttgagcttgt agtctgttgc gttgtaacca
     5881 cttgcaatag cgagggaccc gttgccagtg tctgagactg cggcgccagt ctttactccg
     5941 aagagtgcac ttcccgcagc aacgtttccg tttgtaccat ttgctggttt aattccacag
     6001 agaccgtcgt caacccgctt cagtccgcca cagcttgctg cactgctttt gagattaact
     6061 accactccgt tcgctgcatt tgtagagagc tgcgtgtaga tagtacctga tgagattgcg
     6121 ctcggtacaa gtgcgacacc gcccgtaccg ttatcttcac ccaattttag tgttggggct
     6181 gtaagcgtac ccgtacagcc agaggtgatt ggattaccac cacccgaagc tgaacctgaa
     6241 acacagaata gaagcgactc tgcaacggca ccagacacac caattgtatc tgtaatcgct
     6301 atcgcaacac ttccctggtc ttggacgccc gatccgagtg tggccgatgc gtagttgcct
     6361 gcgtcagttg cattatcgta gctgacaata cgagcataca ttgttgccga agatgaaggg
     6421 tttgtaatac ctgtaaatgt atctgtaatt gactcagcag ccgagattac atttgtaaca
     6481 actagcttat tcgtcgaacc tgtcactgca gttgctgtag tgccggctgc accaagcgct
     6541 gcactcgctg cactaaatcc agtcggtgct gtacatgcat ctggaattac tggtgaattt
     6601 gaacagaatt caacaacata tgcaccagcc gccttagtag ccgtaaatgc gaccttatat
     6661 gatactcccg tcgcactttt tgtcgcacta cttaattcta ttgaacgact cgtaatcgag
     6721 tcagccgata ctagcacagg caacaccgcc tgccctaatg ttgcgaccac caatgccgct
     6781 aaagtagcca tgcggcctgt ccgctggcta atactcctaa aggaactcat attccttctc
     6841 ctcacgtttt ttaaagaaat atttgttttg tcttctcttg acaaggctag tatatcacaa
     6901 tactgctcat gcttgtcaac aatattatat aaaagttttc cacaggttat ctatttttac
     6961 tgttctcgaa agtatcgcgc tctttaagca tcttgttgcg gattgacacc atttggctaa
     7021 gcctgcctag ccagaacgta ggcagaaata atgcaaacat cgtaatcgtt ggcacccaaa
     7081 gcacctgtgg cggcgcctgt gatgcttttc ctgaagaatc accacccaca acacccgctg
     7141 tcgtgctatc tactttgccg ctagaaaccg ctacaacctg taaaaatgcc gatacgccgt
     7201 ctgcatccgt ggctgtgaca gaaaccggat aaatacccgc cttcgagtac gggtggtcaa
     7261 ttggcacaac acccgtgaat gcctggcttt ttagctgcgg ttttgaccca tcgccccaat
     7321 caatactgaa tgcataagga ccgctgccgc ccgatagctg taatggccag gttagcgaag
     7381 taccagcggg cgctgaacgc ctgccatacg aacttgtgag cgttactaat gaaccaaagg
     7441 cgctaaagtg tgtatcgctg taggttatag tgaccgccgc cgaagtaggt ccggtttggc
     7501 ccaaatcatc gaaaacggtc gccgcgagtt catttgaccc tgcaaataag ctcacctgca
     7561 tactgaaact accgccatta cagagtgatg aacccaccat tacaccgtta tcatatagct
     7621 gcacaagtaa accattcgga catataccag caaccgtgat tggtgaattc gtaaatccag
     7681 agccgcttcc tggtgtggag atcgtagcgc cctgagttgg tggcacctgc gttttagacg
     7741 cagctagacc aaatgagccg ggcttaggct ctggatttgg aatctgtgtg attgcattcg
     7801 ctggctgcac aagcaagttg agggcaacta ctgtcgcccc cactatactt agggttcccc
     7861 accgttgaat tgtacgtgct gcgtacatca tgcttatgtc tattgtacag cgtagccgta
     7921 aacggcaaaa ggctgttatt gattacttac gtcgggctga tttggttttg gcaacacgtg
     7981 gaccgtagag tttattgcgg atcttgttgt atgcgcgcca accgtagaag cctgctacgc
     8041 cgagaacaac gaccgctaca ccaatgaacc accatggtag gtaccagaat ccgccaactt
     8101 ttgtcacttc ttgggttaaa ttaccatttt gcccgtagaa gaggtccgca gacacactgt
     8161 agtagcctgg ccataaacct gaagcgtcac atgttgagga ttttgttgta gtattaccta
     8221 gcttcacatc ctcaggtttc aattttacac atgttaagaa cgtgcgctcc tgtccgataa
     8281 gcgctggaga gctagagggg ttaagctcat taatagttgt cgtgcgacca aagatgtcac
     8341 gtagggtaat gttaccagca ggtgcttcaa ctacgtttcc attgttcttt agggtatacg
     8401 caattgtatc tggagcctgt gtattgaagt aatgatagcc tgatagatca ccctgtgccg
     8461 cccgatcgta tgcaccaatg tgcgtaaaat caagattctc tttcacctta ccagggacgt
     8521 ttacgaatac tagtgttaca cctgacgcgt taagtccaat atttccacct tggccacttc
     8581 ccgatgaata cactattgca ctatagtagc tgcccgcacc atggccctta ggtatggcta
     8641 catttaagtc gacttgctta cttttacctg ggtcaattgt tactgtttgc ggaactttta
     8701 aataagaacg tagcgaccaa gtagtttgtg gtgtattagt gtctaaaaat agctttggcg
     8761 taccgccgtt accagtaaaa ctaaagtcga tgacacgcaa tgtgagattc agggctgtcg
     8821 cctgatcaag attcgtaatg ttcaatttat cttgcacgct attgcctggc tcaataacgt
     8881 aattcttttt aggtgcaatg ccaagggccg atgaagagtc agccgctacc tgcgtaggag
     8941 gcaatacata tgtcacgctt agtagtacag caaatacagc cagcactagg ctttttatgt
     9001 gtttcatatc cctctaataa ctcctcatat ttgtagtatt gtaactataa gctgtattgt
     9061 agcactaaaa gcggccgctg gctataaggg tgatcgttgt tgtatacaca ccggctcgaa
     9121 gatctttgct tgagtttaga atgtagctaa ccgtaaactt gcgcatcaag ctaacatttg
     9181 gcgaatacgc cacaacatcg cctgagacaa atttatattt atttggtatc ccataatctg
     9241 ccgatggtac agcgtttgcc catgtaccct ccgggttgtt accaattgta ggcacggtat
     9301 tttcgactag attaatccca aactggtttg tgccttgctg gctttcagtg ggtaatgctg
     9361 gcgagttaat cacactcgta cccgcggaca ttggcgggcc attcgccgtc accacaaatc
     9421 cttgacttgc attcgtccct actgcgagtt gggattgagc tgttaacgta tcgtgagggc
     9481 tcaatgttcc catgtcacga taataggttt catcggtgcc agcacagtta tcatcaacgt
     9541 gttgtgcggc gcagaagaca agcattgggg gtacttgaga ttgaatcatt atccctgttt
     9601 cgacttgtcc acggactgaa ccaaaatcaa tcaacggccc ggtgccatct atagatgata
     9661 gactcttcat acgaatagaa aaggcttggc ttgtatcggt tgggtttacg attccactga
     9721 acgtaaaact tgattcagag gggtaaattg gcactgtagg tgcgcgcgcg agtataatat
     9781 gattctgcgt ttgcgaatgg atagagaaac ccgtctcgcc cgactgattt gttagcgttg
     9841 catgcgatac atctagccct gttggtatcg cacagggcat atacggaatt gggtcgttgc
     9901 aaaatagcat atctaacgac ccaacttgct ctggactcat aaattttaaa gtaactgtat
     9961 aggatgttgt tgcgccaggg tctgtcgtct gcatcaatag cccgcgtgtc tcaaaacggg
    10021 ttgtcgcaga tacgggcacc ataaac
//
LOCUS       DATIIX010000014         3385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_476056, whole genome shotgun sequence.
ACCESSION   DATIIX010000014 DATIIX010000000
VERSION     DATIIX010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476056"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..2864
                     /gene="secA"
                     /locus_tag="VLF64_00405"
     CDS             234..2864
                     /gene="secA"
                     /locus_tag="VLF64_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HSW90439.1"
                     /translation="MVSQQQALTKVFGDPQKRILRRMQKKVNEINKLEETYKKMSKKD
                     LAAQTEVLKKRFSKKGTTLDTLLPDAFALTREMSNRVLGMRPFDVQLIGGIALHEGNV
                     AEMKTGEGKTLVATLPVYLNALEGKGVHVVTVNDYLAQRDAGWMGELYAALGMSVGVI
                     VNDASFLWDGKYDNESHTDARMKKLRPCTRKEAYEADITYGTNNEFGFDYLRDNMVNE
                     IDLVRQRSLHFAIVDEVDSILIDEARTPLIISAPAADNPDSYYQFAKIVAKLVPDDYV
                     LDEKRRSVALTDEGVEKVQKMLGIKNLYTPEYVRSVYHMDQALRAQTLFHRDKDYVVT
                     NGGEVIIVDEHTGRLKQGNRYNEGLHQAIEAKEGVPVLQESMTLATISFQNYFRLYDK
                     LSGMTGTALTEAEEFQQIYELDVVVIPPNRAIARKDHEDLIYKTEKGKLKAVAQAIKE
                     YHDAGRPVLVGSGSIAKNEMIAAFLDKEGIKYEILNAKNNEHEAAIIERAGEKGAITL
                     ATNIAGRGTDIKLGKGVKELGGLVVIGSERHESRRIDNQLRGRGGRQGDPGDTQFYVS
                     TEDDLMRIFQGERIASLMDRLGVDEETAIQNRAVSKTLESAQKRVEGYNFDTRKNVVQ
                     YDNVINRHRKVVYTMRRRILEGDNIKPEIQRLLGELVTDLTLVPGKNNPKFAEQFESV
                     IPASDKEIEEIGLEKKDRARRELALKVAEKCYTVKEKELGSDLIRQVEREVYLQVLDT
                     LWMQHLENMQHLREGIHWRSVGQRDPLVEYRSESQKLFESLQVTLREEVLRAIYHVRK
                     TDAISRTAQEEEHDTELTKLAEKSVEHGVNEITGGEENRDDDFSDSIKKAKTTTEVNH
                     QRNVARKKKKAQRQNRKKSR"
     gene            2867..>3385
                     /locus_tag="VLF64_00410"
     CDS             2867..>3385
                     /locus_tag="VLF64_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HSW90440.1"
                     /translation="MKHTTTEVRLKNGARGLLIDVPGATVMSFQFQFRAGNRYVKNKD
                     IYETAHIMEHMAFGANAEFKSEHAYEAEFTKNGAYHNAWTSDLSMVYIADCADFEWER
                     ILQLQQVAICQPKFRGEELEAEKGNVKSELTGYLNNHNRVLWPKIQQLLGEDVYSYWQ
                     RIQTISNVTIRDI"
BASE COUNT          952 a          684 c          897 g          852 t
ORIGIN      
        1 tgacaaatca gctgcgcaaa tacaaagaag atagtattcc gcatgtaggg caacgtaagc
       61 ttctcgatcg ctttaaacgc agctacgcgc gcgaaatgta gccataaaat gaaaagacag
      121 ccagtaagtg gctgtctttc ttgctctacc tcttttaatc gtgcttgtaa acgggtataa
      181 tgatgggtag tttttctgat agtccacatg agagcagaga gggtttttat cgtatggtaa
      241 gtcaacaaca ggcgctaact aaagtattcg gtgatccgca gaagcggatt ttgcgccgaa
      301 tgcaaaaaaa ggttaacgag attaataagc tcgaagaaac ctataaaaaa atgtctaaaa
      361 aggaccttgc ggcccaaact gaagtgctga agaagcggtt ttctaagaag ggaacaaccc
      421 ttgacacact tttacctgat gcgtttgcgc tgacccgtga aatgagcaat cgcgtgcttg
      481 gcatgcgtcc atttgacgtg cagcttattg gcggtattgc attgcacgag gggaacgtgg
      541 cagaaatgaa aactggtgaa ggtaagactc tcgttgcaac tttgccagta tatttgaatg
      601 cgcttgaggg taagggtgtt cacgtagtga cggtaaatga ctaccttgca cagcgtgacg
      661 ctggctggat gggtgaactc tacgcggcgc ttggcatgag tgtcggtgtt attgttaatg
      721 atgcatcgtt cctttgggat ggtaaatacg ataacgaaag ccatacagat gcacgtatga
      781 aaaagctccg tccatgcacc cgtaaagagg catatgaagc ggacattacc tacggtacta
      841 acaacgaatt tggatttgat taccttcgtg acaatatggt gaacgagatt gatctggtgc
      901 gtcagcgtag tttgcacttt gcaattgtgg acgaggtgga ctcaattctg attgacgaag
      961 cacgtacgcc gctgattatt agtgcgcctg ctgcagataa tccagactcg tactatcagt
     1021 tcgcaaaaat tgtcgctaag ctcgtacctg acgattacgt actcgatgaa aagcgccgta
     1081 gtgttgcgct tacagacgaa ggtgtcgaga aggttcagaa gatgcttggc attaaaaacc
     1141 tttacacgcc tgaatacgtt cgtagcgtgt atcacatgga tcaagcgctt cgtgcgcaga
     1201 cgctttttca ccgtgataag gattatgttg ttactaacgg tggtgaggtg attatcgtcg
     1261 atgaacatac cgggcgcttg aagcaaggta accgctacaa tgaaggcctt caccaggcta
     1321 ttgaagcgaa agaaggtgtt ccggtattgc aagagagtat gacacttgcg actatttcat
     1381 tccagaacta tttccgtttg tacgacaagc tttcagggat gaccggtacg gcacttactg
     1441 aggccgagga attccagcag atttatgaac ttgacgttgt cgtgattcca ccaaatcgag
     1501 ctattgctcg taaagatcac gaagatctta tctataagac tgagaagggt aagttgaagg
     1561 cggttgcgca ggctattaag gaatatcatg atgcgggccg acccgtgctt gtcggttctg
     1621 gatcgatcgc aaagaacgaa atgattgccg cgttcctcga caaagaaggc attaaatatg
     1681 aaatcttaaa tgctaagaat aatgagcatg aagcggctat tatcgagcgg gctggtgaaa
     1741 agggcgcaat tactcttgcg acaaatattg ccggtcgtgg taccgacatt aaacttggca
     1801 agggtgttaa agagctcggc ggtttggttg tgattggctc tgagcgtcat gaatctcgtc
     1861 gtatcgataa ccagcttcgc ggtcgtggcg gccgtcaagg tgaccctggt gatacgcagt
     1921 tctacgtatc gaccgaagat gaccttatgc gtattttcca gggtgagcgt attgcaagct
     1981 tgatggaccg acttggtgtt gatgaggaaa cggcaattca gaatcgtgca gtgagtaaaa
     2041 cactcgaatc tgcacagaag cgtgtagagg gttacaattt tgacactcgt aaaaatgttg
     2101 ttcagtatga caatgtgatt aaccgtcacc gcaaagttgt atacaccatg cgtcgtagga
     2161 ttcttgaagg tgataacatt aagccggaga ttcagcgttt gcttggcgaa cttgttaccg
     2221 acctaacgct tgttccaggc aagaataatc ctaagtttgc tgagcaattt gagtcagtta
     2281 tcccagcttc tgataaggaa attgaggaga ttggacttga gaagaaggat cgcgcacgtc
     2341 gcgagctcgc actgaaggta gccgaaaagt gttacaccgt taaggaaaaa gagctcggta
     2401 gcgatttaat tcgccaagtt gagcgtgaag tctacctaca ggttcttgat acgctttgga
     2461 tgcagcacct cgagaatatg caacatcttc gcgagggcat tcactggcgc agcgttggtc
     2521 agcgcgaccc acttgttgag tatcgctcag aatcgcagaa gctctttgag agcttgcagg
     2581 tgacactccg cgaagaagta ctgcgtgcaa tttatcacgt acgcaaaact gatgcgatta
     2641 gccgtactgc ccaggaggaa gagcatgaca ctgagcttac gaagcttgct gaaaagtcag
     2701 ttgaacacgg tgtgaacgag attactggtg gtgaagagaa tcgtgacgac gactttagcg
     2761 actccatcaa gaaagcgaag acaacaactg aagttaatca tcagcgcaat gttgcccgaa
     2821 aaaagaaaaa ggcacagcgc cagaatcgta agaagagtcg ttaggcatga agcacaccac
     2881 cactgaagta cggttgaaaa atggtgcccg cggtttgttg attgatgttc ccggcgcgac
     2941 ggtgatgagc tttcagtttc agtttcgtgc cggtaatcgc tacgtaaaaa acaaggatat
     3001 ttacgagacc gctcatatta tggagcacat ggcgtttggt gcgaacgctg aatttaaaag
     3061 cgagcatgcg tatgaggctg agtttactaa aaacggtgct taccataacg catggacgag
     3121 cgatttgtcg atggtatata ttgctgactg tgctgacttt gaatgggagc gtatcttgca
     3181 attgcaacaa gtcgccattt gtcagcctaa attccgcggc gaagagctcg aggcggaaaa
     3241 gggtaatgtg aaaagcgagc ttacgggcta tcttaacaac cataaccgtg tgctttggcc
     3301 gaagattcag cagcttcttg gcgaagacgt atatagctac tggcagcgta tccagacgat
     3361 tagcaatgtg acgattcgtg atatt
//
LOCUS       DATIIX010000015         4250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_392799, whole genome shotgun sequence.
ACCESSION   DATIIX010000015 DATIIX010000000
VERSION     DATIIX010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392799"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="VLF64_00415"
     CDS             <1..1352
                     /locus_tag="VLF64_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HSW90441.1"
                     /translation="VGRVQATRKFGKLAFLVLRDMSGQIQLFLKADTVAPLDGAKSQI
                     GMSELNLLDPGDFVEATGLVIRTKTGEISVDVRELRLLTKSLRGMPTEQEGFTNKEER
                     LRRRYVDMNVNLEVRERFVRRSKFWRATRKFLDDRGFHEINIPVLEHTTGGADANPFV
                     THMDALDQDFYLRISHELPLKRLLGAGFEKVYDVGPRFRNENYSDEHLPEHIAMEWYW
                     AYADWQDGMKFMEEMYKYVLKETFGTLQFNVRGLEVDMDKEWEVWDYATVIRDHYGID
                     VYNTTIEEVSARLKEHKLEVEKTDSIPRGIDKLWKNIRKDVVGPVWLVNTPKFISPLA
                     KSNTENPNVVQRFQPVILGSELGNGFSELNDPIDQYNRFIEQQQMRDQGDDEAMMLDI
                     DYIEMLEYGMPPACGWGSSERVFWMFEGVTAREGVPFPQLRHEIDETTRALYPEAQL"
     gene            complement(1364..1663)
                     /locus_tag="VLF64_00420"
     CDS             complement(1364..1663)
                     /locus_tag="VLF64_00420"
                     /inference="COORDINATES: protein motif:HMM:NF039821.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5663 domain-containing protein"
                     /protein_id="HSW90442.1"
                     /translation="MPLLSPTLLENLGINLSEEHYHALAEHFETTLNERVINEIVLEL
                     SPEQAEQLARLQQAGDEEILAWLKANVPDVAEIVADEVDILLGEIAENSEAISDS"
     gene            complement(1683..2975)
                     /locus_tag="VLF64_00425"
     CDS             complement(1683..2975)
                     /locus_tag="VLF64_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90443.1"
                     /translation="MTEKILRSAYEVANDKYPAPDAATASAKSTPDTAPIPAQSTSPE
                     NSVPTSIDTAPVVPPSPDNLSTPDINPTTNTETFATDVEILGKHYPVHQMINEIGNQR
                     MLAVESVSASWKNMLDTPGKMRLGLARSIAEGRLTRQQRKADAVSHLSNRNWLRRRRM
                     NKLRKAKERFDGANDKYQGRKDRMTARRENVGKNAEQRRNEYRKELRARREAALGRRA
                     LRHELRAQDAGRLEARAVLKDIPKERLDRVGKLAATAYASERRASQARRQEKTAISRE
                     TRTLNAISDNRRRSAEAASEAKKADSIVEKLQAETIPRAEASLQSLRNNLEGLEKDDP
                     ARGNLEVQIREAEDRIAMYQQREIPYWSQVALSNRERIIRLDENHKTLTTILGHDTAA
                     KNTAVTDTNAARTTAEAHKTELAKAATEATNSGPESNN"
     gene            3092..3424
                     /locus_tag="VLF64_00430"
     CDS             3092..3424
                     /locus_tag="VLF64_00430"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HSW90444.1"
                     /translation="MQESERYAEQMATQLRKGFLVYCVLKVCLHEPKYTSDIITRLRE
                     AHLVVVEGTIYPLLARLHRDGLLQHEWQESEQGPPRKYYKITEFGEEVVDELTKKIAE
                     LDRTLKKL"
     gene            3435..>4250
                     /locus_tag="VLF64_00435"
     CDS             3435..>4250
                     /locus_tag="VLF64_00435"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HSW90445.1"
                     /translation="MKEITRIHIARTPYSAEIDAKKVLEEYLAAIKKSLSADDDAMRE
                     IEARIVEILAARGIGNEGVITHTDIEAVEAQLGAPSDFADEDAAETPFESGAGERRLM
                     RDTSRGVLGGICAGIADYYGVNVIWPRLVAVLIALITSGTAVFVYIVMWVVIPPARTA
                     AEKLQMRGRPVTLAALKSESKEETAEVPERSKPFVIFVRVLLGFGFVGMALMAIAMLI
                     AVIVVRKPLFGYSLHDLSSGGVLTSIGTAYVGVIIAAVLFILLMGLSAYASFAW"
BASE COUNT          978 a          859 c         1187 g         1226 t
ORIGIN      
        1 tagtggggcg tgtacaggca actcgtaagt tcggcaagct cgcgtttctt gtgcttcgtg
       61 atatgagtgg ccagattcag ctgtttttga aggctgatac tgtggcgcca cttgatgggg
      121 ctaaatccca gattggtatg agtgagctta atttgctcga tccaggcgat tttgttgaag
      181 caaccggcct ggtaattcgt acaaagacag gcgagattag tgttgatgtg cgcgagttga
      241 ggttgttgac gaagagcctt cgtggcatgc cgacagaaca agaaggcttt acgaacaaag
      301 aagagcgctt gcgtcgtcgt tacgtcgata tgaatgttaa tcttgaagta cgtgagcgtt
      361 ttgtgcgacg tagtaagttc tggcgggcaa cccgtaagtt tcttgatgac cgaggttttc
      421 atgaaattaa tatccctgta cttgaacata caacgggtgg tgctgatgca aacccatttg
      481 tgacgcatat ggatgcactc gaccaagact tctaccttcg cattagccat gagttaccgc
      541 tgaagcgttt gcttggtgcg ggatttgaaa aagtttacga tgttgggcca cgcttccgca
      601 acgaaaatta cagtgacgaa catttaccag agcacattgc gatggaatgg tactgggcgt
      661 atgctgattg gcaagacggc atgaagttta tggaagaaat gtacaaatac gtgctcaaag
      721 aaacatttgg tacattgcag ttcaatgtac gcggccttga ggttgatatg gataaggagt
      781 gggaagtatg ggattacgcg acggtgatcc gtgaccacta cggtattgat gtatacaata
      841 caacaattga agaagtaagt gcgagactta aggaacataa gcttgaggtt gaaaagactg
      901 atagtattcc acgcggtatt gataaattat ggaagaacat ccgtaaggac gttgttggcc
      961 cagtgtggct cgtaaatacg ccaaagttta tttcgccact tgcgaaatct aacactgaga
     1021 acccgaatgt tgtacaacga ttccagccag taattcttgg tagtgagctt ggtaatggct
     1081 ttagcgagct taatgatccg atcgatcagt ataaccggtt tatcgagcaa cagcagatgc
     1141 gtgaccaagg tgatgatgaa gcaatgatgc tcgatattga ttatattgag atgcttgagt
     1201 atggtatgcc accagcatgt ggatggggta gcagtgaacg cgtattctgg atgtttgagg
     1261 gtgtaacggc acgcgaaggt gtaccgttcc cacagctgcg tcacgaaata gacgagacaa
     1321 ctagagcatt gtaccctgaa gcgcagttat aataactgcg cttttagctg tcgctgatag
     1381 cttcgctatt ttcagcgatt tcaccgagga ggatatcgac ttcgtcggca acgatttcgg
     1441 cgacatctgg tacgttagct ttaagccaag cgagaatctc ttcgtcacct gcctgctgta
     1501 acctggcgag ttgttctgct tgctcgggtg aaagctcaag gacgatttca ttgatgacgc
     1561 gttcgttaag tgtcgtttca aaatgctcgg cgagtgcatg ataatgttct tcgctcaggt
     1621 tgatcccaag gttttcgagt agggtagggc taagtagtgg catatgttgg atcctttcag
     1681 ggttaattgt ttgactctgg gccgctgttg gttgcctcgg tagctgcttt agcaagctca
     1741 gttttatgtg cttcagcggt cgtgcgcgcg gcattcgtgt ctgtgactgc agtgttcttg
     1801 gcggcggtgt catggccgag gattgtggta agagttttgt gattttcatc aagtctgata
     1861 attcgctcgc gatttgagag tgcaacttgg ctccagtatg gaatctcacg ttgctggtac
     1921 atggcgatgc ggtcttcggc ttctcggatt tgaacttcta gattgccgcg agctgggtca
     1981 tctttctcta gcccttcaag attattccgg agtgattgga gcgatgcttc agctctagga
     2041 atcgtctctg cctgaagctt ttctacgata ctatcggctt tttttgcttc gcttgcagct
     2101 tcagcgctac gccgacggtt atcgcttatt gcgttgaggg tgcgggtttc gcgactaatt
     2161 gcagtttttt cctgcctgcg cgcctgactc gctctgcgct cgcttgcgta tgcggttgcg
     2221 gcgagcttac caactcggtc aaggcgctcc ttagggatgt ctttgagcac tgcacgtgcc
     2281 tcaaggcgac ccgcatcttg cgcacgcaat tcatggcgta gcgctcggcg accaagcgct
     2341 gcctcgcgac gagcgcgtaa ttctttgcga tattcattgc gacgttgctc ggcattctta
     2401 ccgacatttt ctcggcgggc agtcatgcgg tctttacggc cttggtattt atcgtttgct
     2461 ccatcgaatc gttccttggc tttgcgcaac ttgttcatgc gacgtcgcct aagccaattt
     2521 ctgttgctaa ggtgactgac tgcatccgcc tttcgttgct ggcgagtaag gcggccttcg
     2581 gcgattgatc gtgctaaacc gaggcgcatt ttcccaggag tatcgagcat gtttttccag
     2641 ctggcgctta cactttcgac ggcgagcatt cgttggttgc caatctcgtt aatcatctga
     2701 tggactggat agtgtttgcc cagaatctct acgtcagtgg caaaggtttc tgtgtttgtt
     2761 gtggggttaa tgtctggtgt tgatagattg tcaggtgaag ggggtactac tggtgctgtg
     2821 tctattgagg tggggacgga gttctctggg gaagttgatt gcgccgggat tggcgctgta
     2881 tctggtgttg actttgctga ggcagttgcg gcgtcgggag ctgggtattt gtcatttgcg
     2941 acttcgtatg ctgatcgcaa tattttttca gtcatgttac ttttcagttt ccatttattc
     3001 tatatagttt atcgtagcat aagctgtatg gggtcacaag aagaaattat aacctgttga
     3061 caataccaag taccttgtat aacatagtag tatgcaagaa agtgaacgat atgctgaaca
     3121 aatggcgacg cagttgcgca aaggcttttt ggtgtactgc gtgctgaagg tttgtttaca
     3181 tgaaccaaag tatacgagtg atattatcac gcggcttcgt gaagcacacc ttgtggtggt
     3241 ggaagggacg atctatccat tactcgcgcg gcttcatcgc gatgggctat tgcagcacga
     3301 atggcaagaa agcgagcaag ggccgcctcg gaagtactac aagattaccg agtttgggga
     3361 ggaagtagtg gatgaactca ccaagaaaat tgccgagttg gatcggacat taaagaaatt
     3421 atagaggaag aatcatgaaa gagattacaa gaattcacat cgcgcggaca ccatacagcg
     3481 ctgaaatcga tgcaaagaag gtgcttgagg agtatctggc ggcaattaaa aagtccctca
     3541 gtgctgatga tgatgcgatg cgtgagattg aagcacgaat tgttgaaatt ctggcggcta
     3601 gaggaattgg aaatgagggt gtgattaccc atacggatat tgaggcagtg gaagcacaac
     3661 tcggtgcgcc aagtgatttt gctgatgaag atgcggcaga aaccccattt gagtccggcg
     3721 ctggcgaacg acgccttatg cgtgatacat cgcgcggtgt tttgggtggt atttgtgctg
     3781 gaattgccga ctactatggt gtaaatgtga tatggccacg actcgttgca gtgctgatcg
     3841 cacttattac gagcggaact gcagtatttg tttacattgt gatgtgggtg gtgattccgc
     3901 cagcacgaac tgctgctgaa aagttacaga tgcgcggtag gccggtaacg ctcgcagctt
     3961 taaaatctga gtccaaagaa gagacggccg aagtgccaga gcgatcaaaa ccattcgtaa
     4021 tttttgtacg cgttttgctt ggtttcggat ttgtcggtat ggctcttatg gcaattgcga
     4081 tgcttattgc tgtcattgtt gtacgtaagc cactatttgg gtactctctt catgaccttt
     4141 cgagtggcgg tgtgcttaca tctattggta cggcctatgt tggcgtaatt attgcggcag
     4201 tcttatttat acttttaatg gggcttagcg catatgcgag tttcgcttgg
//
LOCUS       DATIIX010000016         3970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_309840, whole genome shotgun sequence.
ACCESSION   DATIIX010000016 DATIIX010000000
VERSION     DATIIX010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309840"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VLF64_00440"
     CDS             <1..339
                     /locus_tag="VLF64_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90446.1"
                     /translation="IEELIAIIQSKTAKDRHDPARIILVSPIYVDDQASTFTKFYVPD
                     FYNHESALKSQGLSDELQKVATGAGCSFVDAAKIAHAGEDGIHFSQEAHAALAKALIE
                     EVRTLAEAKG"
     gene            482..1039
                     /locus_tag="VLF64_00445"
     CDS             482..1039
                     /locus_tag="VLF64_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HSW90447.1"
                     /translation="MHDNELWQVYNNNGTPIEGKGASKQEFNANANLIMGNAHIWFWK
                     TDGTTTEVMLQKRSLNKSNYPGWYHISAGGHINVGETPIDAAVREAQEEMGLAINPSK
                     LHYVHSVRIIPRDPRDIVNVYLYRLNGDEEITHLDGEVDSYEWRTIENWKEITKDAEN
                     NNLVPQGQLYFDTLTKALELIASKI"
     gene            1164..2585
                     /locus_tag="VLF64_00450"
     CDS             1164..2585
                     /locus_tag="VLF64_00450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSW90448.1"
                     /translation="MSKTAKTQTIKGLAPGLVLIGLVSAAMSSLGAPLVPTVVKVLHV
                     HLGVAQWTLTISLLVGAVITPVLARLGDGPHRRRVILVTLWAVLAGSVLATLPLGFIG
                     LMVGRALQGVGLGLSALIVGVAKDHFAGNKGRRIVALLSVTSLAGVGFGYPIAGAIAQ
                     FAGLRAAYGIGIVMAAAALVFAYRTLPHERHRVARKLDTTGAGLLGLALALLLYTLTQ
                     VVGRIQPDVVVIGIVAISLFIGWIWYERSQSEPLVQLNTLRHPAVFLADGVVLLASIG
                     MYLLIATAVRYVQTPTTVVYGLGASVLTTGFMLTPFSIFGFVASRVVPILRCTMHDKQ
                     VLALSTVVVLISLVGFAAFRSQLWEVFVWIALAGLGIGAVYAAIPALIIDAVPAHITT
                     SALGFNQVLRMVGYSIGSALSALILEAYTPSGNMLPLGQGYTAVAIVGIITSIATVCI
                     ILVFHIRQLRHKLVPVEELQIID"
     gene            complement(2643..3434)
                     /locus_tag="VLF64_00455"
     CDS             complement(2643..3434)
                     /locus_tag="VLF64_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90449.1"
                     /translation="MPTRLPVTGSDEGQWGDILNAFLLVAHASDGTILPGSVAAAGAE
                     ITSNKGQSNGYAPLNSSTQIASTYLPIGTTASTVAAGNDSRFAEVNGVAVSGAAAAGK
                     VLTASSSSAASWQTPSAVASRQVVIALSGTSSSQVATVGTWNSIYLTNTDTANFVGWV
                     NSSDTAQNNSITFDFACNAGTHTLEFFHLPFQNRGIYTLQIDGTTIGTVDGYSGGLVP
                     TRSVLTGITVSTSGQHTFTILMATKNASSSGYQGMMDRAVFTQTA"
     gene            complement(3547..>3970)
                     /locus_tag="VLF64_00460"
     CDS             complement(3547..>3970)
                     /locus_tag="VLF64_00460"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HSW90450.1"
                     /translation="VLLIILAPTLFIKKQSLQPGERSKQSMLFGYGIYAVILFASSLG
                     SGLATLLFLPLMFLMGLTTMQANALRRVLMLLQAAIAFCMVLPQGYIVWSYAIASLLG
                     CYGGGFVGTKIALKHGEHFIKYALASIMVVSGVALLIK"
BASE COUNT         1081 a          920 c          931 g         1038 t
ORIGIN      
        1 atcgaagagc tcattgcgat cattcaaagc aaaactgcaa aagacagaca cgacccggct
       61 aggattatac ttgttagccc tatttatgtt gacgatcaag catcaacctt taccaagttc
      121 tacgtgccag acttttacaa tcacgagtca gccctaaagt cgcaagggtt gagcgatgaa
      181 ttacaaaagg tcgcaaccgg cgccggttgt tcttttgttg acgccgccaa aattgctcat
      241 gcaggagaag acggaattca ctttagtcaa gaagcgcatg ccgctctagc aaaggcgctc
      301 attgaagaag tgcgaacttt ggcggaagcg aagggctaat gaagataacc aactcacatg
      361 aaactgggca cacagtctct tccagaatca cctaatttta tttatcataa gcatatcgtt
      421 aaagcttctt cggcaatttc aagtagcaac attcaattta tataaaggaa gcacatttct
      481 tatgcatgac aatgaactct ggcaggtata caacaataac ggcactccaa ttgaaggtaa
      541 aggtgcttca aaacaggaat ttaatgccaa cgctaacctt attatgggta acgctcatat
      601 ttggttttgg aagacggacg gcacgacaac agaggttatg ctccaaaagc gctcactaaa
      661 taagtcaaac tatcccggct ggtatcatat ttccgcaggt ggccacatta atgtaggtga
      721 aacaccaatc gacgcagcgg ttcgcgaagc gcaagaagaa atggggcttg ctatcaatcc
      781 ttcaaagctt cactatgttc actcggttcg tattatccct cgcgatccgc gtgatattgt
      841 aaatgtttac ttgtacaggc taaacggcga cgaggaaatt acacaccttg acggtgaagt
      901 tgattcctat gagtggcgta ctatagagaa ctggaaagag attacaaaag acgctgagaa
      961 taataatctt gtgccacagg gccagctcta ttttgacact ttaacaaaag ctctagagtt
     1021 gatagcatca aaaatctagt tccttcgaag caacctgcta agtggaagga tacataaata
     1081 agtcctaaga catctataaa aagatggata acatcatcca tttcttgact atggaaaacg
     1141 cctggtagct tatactggaa aagatgagca aaacagcaaa aacacaaaca ataaaaggac
     1201 tagcgccagg gcttgtcttg atcggcctag taagtgctgc catgagcagc ctcggtgcgc
     1261 ctctcgtgcc aacagtggtg aaagtgctgc acgtccactt gggcgtcgcg caatggacgc
     1321 ttacaatcag cttattagtc ggcgccgtta taaccccagt acttgctcgg cttggtgatg
     1381 gcccacatcg acggcgtgta atactggtaa cattatgggc tgtactcgca ggaagtgtgc
     1441 tagcaacgct gcctcttggc tttatcggtc taatggttgg tcgtgccctg caaggcgttg
     1501 gactggggct gtctgctctt atagtgggag ttgcgaaaga tcactttgcg ggtaataaag
     1561 gacggcgaat tgtcgcacta ttatccgtta catccctcgc gggcgtcgga tttggctatc
     1621 cgatagccgg tgcaattgca caatttgctg gcttacgcgc tgcgtatggc attggcattg
     1681 ttatggcggc agctgcgctc gtatttgcct atcggacact gccacatgag cgccatcgag
     1741 tggcgcgcaa gcttgatact acgggggcag ggctactcgg cctggcatta gcgttattgc
     1801 tctatacgct cacgcaagtt gttggccgca tacaacccga tgtcgtcgtt attggtattg
     1861 tggcaatatc actattcata gggtggatat ggtatgaacg ctcccagtcg gagccgcttg
     1921 tgcagctcaa tacgttacgc catccagccg tttttctcgc ggatggggta gtactcctag
     1981 ctagtattgg aatgtatctg ctcattgcaa cagcggttcg ctatgttcaa acgccaacaa
     2041 cagtagtcta tggtcttggc gcatcagtgc ttacaaccgg atttatgctt acgccattct
     2101 caatcttcgg atttgtagcg agccgcgtag tgccaatatt acggtgtacg atgcacgata
     2161 agcaggtcct tgccttgagc acggtagttg tcctcatttc acttgtcggc tttgcagcct
     2221 ttagaagcca actatgggaa gtgtttgtat ggattgcact tgcagggctg gggattggtg
     2281 cggtctatgc tgccatacca gcgctcatta ttgacgcggt gcctgctcac attaccacaa
     2341 gtgctctagg ttttaatcag gtattgagaa tggtcggata ttcaattggg agcgctctgt
     2401 ctgcattgat actggaggca tatacgccga gtggcaatat gctaccactc ggccaaggct
     2461 atacggccgt agccatcgta ggcattatca ctagtatagc tactgtctgt ataattttag
     2521 tattccatat tcgacaactt cggcataaac tagtgccggt agaagaacta cagattatag
     2581 attaaaccca gtacacattt gtcctgggtt taaatctcta gataggggat gagtcacacc
     2641 cattatgctg tttgtgtaaa tactgcgcga tccatcattc cttggtagcc agaggaagaa
     2701 gcatttttgg tcgccataag aatagtaaac gtatgttgcc cgcttgtgct cactgttata
     2761 cccgtcaata cgctgcgcgt agggacaaga ccgccagaat atccatcaac agtaccaata
     2821 gtcgtaccat ctatctgaag cgtgtaaatg ccgcgatttt ggaatggtaa atgaaagaac
     2881 tctagagtat gagttcctgc attacaagca aagtcaaatg taatcgagtt gttttgtgct
     2941 gtatccgatg agttgaccca accaacgaaa ttggcggtat ccgtgttggt cagatatatt
     3001 gaattccagg tgccaaccgt cgctacttgc gaagaacttg tgccacttag tgcaatgaca
     3061 acttgacgcg aggcaactgc acttggagtt tgccaagatg cagcactact actagacgct
     3121 gtcaggactt tgccggcagc agcagcgcca ctaaccgcta ccccattaac ctcagcaaag
     3181 cggctatcat tgcctgccgc caccgtgctc gcggtagtcc caataggcaa ataagtgctt
     3241 gcaatctgtg ttgagctatt gagtggcgcg tatccattgg attggccctt gttgcttgta
     3301 atttcagcgc cagcggcagc cacgctgcct ggaagaattg tcccgtcact tgcgtgtgca
     3361 actaacagga atgcgttaag gatatctccc cactgacctt catctgatcc cgtaactggt
     3421 aatcgtgttg gcatgctaat tctacttcaa tggttatgtt tagtaattct tattgtacta
     3481 tgcacgaaaa ctctatacaa gcacctatca ataaaaatac atgattcatt aatatagagt
     3541 gcgccactac tttataagta acgccacgcc tgaaacgacc attattgatg cgagtgcata
     3601 cttgataaaa tgctcaccat gtttgagcgc aatctttgta ccaacaaacc caccaccata
     3661 gcaccctaaa agcgatgcga ttgcatagct ccatacaata tagccttggg gcaaaaccat
     3721 gcaaaacgca attgccgcct gtagaagcat cagtacgcgc cgcagcgcat ttgcttgcat
     3781 agtagtaagc cccatcagaa acataagtgg gagaaagagc aacgttgcaa ggccgctacc
     3841 gagtgagctg gcaaaaagaa taacggcata tataccgtaa ccgaaaagca ttgactgctt
     3901 tgacctttcg cctggctgta ggctctgttt tttaataaat agcgtcggtg ctagaataat
     3961 cagcagtacg
//
LOCUS       DATIIX010000017         2524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_143229, whole genome shotgun sequence.
ACCESSION   DATIIX010000017 DATIIX010000000
VERSION     DATIIX010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143229"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VLF64_00465"
     CDS             complement(<1..831)
                     /locus_tag="VLF64_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90451.1"
                     /translation="MTNLDLALYGENPPSSPERAADPARYKFVFWEHDPANFGAAAAA
                     LYDCDVVAIENVGGPSQAERVTLTHDINVINSVSAGSDEKREARERIIASGDAPFLEM
                     VEGITHEGQEICIIDTDENDPSYKDTQEFIDSGIALENAILELELDAELRQLLITNIL
                     AGVAMSRGRNPVLAEQIQAIGDARPGVLVGIGLGAGYHTQAHHMVARRRATARVFAGL
                     SSGTNTDSETINFSYQEDITRQIDLLGRSIDNVGPHLMDRALLENIFGAHGFSTYAAP
                     E"
     gene            complement(1289..1365)
                     /locus_tag="VLF64_00470"
     tRNA            complement(1289..1365)
                     /locus_tag="VLF64_00470"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1329..1331),aa:Asp,seq:gtc)
     gene            1624..2370
                     /locus_tag="VLF64_00475"
     CDS             1624..2370
                     /locus_tag="VLF64_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90452.1"
                     /translation="MSDRSPLYNLYRVGEMHLRAYNRGRSGDTMPPERARQFLGYLAA
                     NPDHQGNFGDRLQGALQWTRQNPLMFMSMVEAFDSAVTFLNTSAGSVDSEAFPNLTEY
                     FRLRGDRQLPYIGNRSSRTPHRPADILHAAQDSTNKLHWGETILPGSDDVYGFHAFMH
                     SNRGMVAASMTDLYENYLWCTGWYPADSAIPALMANAITEAAFIVDHRSPLFHAYRNP
                     EDGWIRGYDGFSEKFQRMVYGPLPPGPAQA"
BASE COUNT          557 a          665 c          657 g          645 t
ORIGIN      
        1 ttcgggtgca gcgtatgttg agaagccatg ggcgccaaaa atattctcga gcaaggcacg
       61 atccataagg tgtgggccta cgttatctat actacgtccc agcaaatcga tttgcctcgt
      121 gatgtcctct tggtaggaaa agttgatggt ttcgctatct gtatttgtcc cactgctgag
      181 tcccgcaaag acccttgccg tcgctctgcg acgagcaacc atgtggtgtg cttgcgtatg
      241 atatcccgcg cccagtccga tgcccactaa aactccaggg cgagcatcac ctattgcctg
      301 tatctgctct gccaggacag ggtttcggcc tctagacatg gccacaccag ccaggatatt
      361 tgtgataagc agctggcgta attctgcatc cagctcaagc tcaaggatgg cattctcgag
      421 cgcaattccg ctatcgataa actcctgggt gtctttgtaa ctgggatcgt tctcatctgt
      481 atctatgatg catatttctt ggccctcgtg agttatgcct tcgaccatct ctaggaaggg
      541 cgcatcccca ctggcaatta ttcgctcccg agcctcacgc ttttcgtcag aacccgccga
      601 tacagagttt attacattga tgtcatgtgt gagtgtaacg cgttccgcct gtgatggccc
      661 tcccacattc tcgatcgcta ccacgtcgca gtcatatagt gcggcagcgg ctgctccaaa
      721 attagcagga tcatgctccc aaaaaacgaa tttataacgt gcggggtcgg ctgctctctc
      781 ggggctggac ggagggttct ctccataaag ggccaagtct aggtttgtca tgtatgtatg
      841 cttttagtgt taataaaaca ttagcataaa actgcttaag tttcaaattg cgtcatgctg
      901 gttgccctat cagatgaacg cttcgctact gcctttactg accggatagc gcacttcgtg
      961 cttttccgcg aagctgtcct cggtgcaagc acctgcggac ggttttgctc cgccgaaccg
     1021 gcgatctacg ctcgctctgc tcgctgatcg ccaggtttgg atccgccggc gcctggcaag
     1081 aaaaatgacc tagcgaatgc tgagtcattt ttcttggcgg gcttgggccg gatagcgggc
     1141 ttcgcccttt atctgcaccg ccatgctcgg agcaagctcc tgtgcgtggc agtgctccaa
     1201 cgaaccgttg ccttcggcac ggttcgactc gcagacgatg tctgcccaaa gtaaaaggcc
     1261 caaaccttac ggcttgagcc ttttactttg gcgggcccga ccggattcga accggcgatc
     1321 tcctccgtga cagggaggcg tgataggcct cttcactacg agcccctagt aatgagacaa
     1381 accgttccgg tttttctcac caacgcgggg cggcggaatg aatccgccgt ctccggctgc
     1441 tcttttccgg gaacaattta actcatcaaa tctcaaacac tagtcacggt acggtttgaa
     1501 atttggcttt ggctgcaggg cagcaaagta acaggggtaa ttttagcaag ggtaggggca
     1561 aaaagcaatc ccggaggtca ataggttgag tatagtagat tatgattcta taatatgcca
     1621 ttgatgtccg acagatctcc tctgtataat ctttaccgcg ttggcgaaat gcacctgagg
     1681 gcatacaaca ggggccgtag cggcgatacc atgccgcctg agcgggcccg tcaatttctg
     1741 ggatacctag cggccaaccc tgaccaccaa ggaaattttg gtgatcgttt gcaaggtgct
     1801 ttgcaatgga cgcggcaaaa tccgctcatg ttcatgagta tggtagaggc atttgatagc
     1861 gccgtgacct ttttaaatac tagtgccggt agtgtcgaca gcgaagcttt tcccaactta
     1921 acagagtact ttaggctgcg tggcgatagg cagctaccat atataggcaa tcgatctagc
     1981 aggacaccgc accgtccggc tgacatctta cacgctgctc aggactctac caacaagcta
     2041 cattggggcg aaaccatctt gccgggtagc gatgatgtgt atggcttcca cgcatttatg
     2101 cattccaacc ggggtatggt agcggccagc atgactgatc tttacgaaaa ttatctttgg
     2161 tgtacaggat ggtacccagc tgatagtgcc attccggcgc tcatggccaa tgctattacc
     2221 gaagcggcat tcatagtcga tcatcgtagt cccttatttc atgcataccg gaatcctgag
     2281 gacggttgga tacgtggcta tgacggcttc agcgaaaagt tccagcgaat ggtgtacggg
     2341 ccactaccac ccgggccagc ccaggcgtag tcccggctac gccttgctta aggctatccg
     2401 ttcgcttgga cctgcagtat agtaggggca tggtaaacat aaaaagtttg cacaacccgg
     2461 gtttgggcgt ttggatcttg cgcctgggcc tagcatttgt gttcggctac gctggtgtgg
     2521 cctc
//
LOCUS       DATIIX010000018         9861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_282262, whole genome shotgun sequence.
ACCESSION   DATIIX010000018 DATIIX010000000
VERSION     DATIIX010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 9861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9861
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282262"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VLF64_00480"
     CDS             complement(<1..241)
                     /locus_tag="VLF64_00480"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90453.1"
                     /translation="MSHQIKKNLGVTLSHGKAEFRVWAPFAEKVAIGGTFTPGGALDM
                     ESEQDGYWSITIKDVEPGHQYKYYFTTKDGHTLEKN"
     gene            314..607
                     /gene="gatC"
                     /locus_tag="VLF64_00485"
     CDS             314..607
                     /gene="gatC"
                     /locus_tag="VLF64_00485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HSW90454.1"
                     /translation="MSTITTNDVRHLAQLSNLQLSDDEITSLQTDLANILDYINQLNE
                     IDTTGVEPTYQVTGLENVWRDDTVDTGTVTRENLLNLTPDQTDNSIKVPQVLA"
     gene            604..2010
                     /gene="gatA"
                     /locus_tag="VLF64_00490"
     CDS             604..2010
                     /gene="gatA"
                     /locus_tag="VLF64_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HSW90455.1"
                     /translation="MSIRTLVARVNNGQTTARALVEESLQKARKAEEYHALLSLTEER
                     ALARADEIDARIKGGEQVGLLAGIPFVVKDNFLAFGAPTTAASKMLENFEAPIQATVV
                     EKLEQAGAICIGKSNLDAFAHGGSTENSAFGMTHNAIDKTKVAGGSSGGSAVVTALDI
                     VPFALGSDTGGSIRQPASFNGVFGMKPTYGMSSRYGVVAMASSTDVMGCFAKSADDVA
                     LIESVMAGQDAKDMTTLPDYFSPLARVPAGLKVGLVKEAMTDDVDPEVRRATEQYAEK
                     LRAAGHTVDEVSLPSVKHSLAIYYIVVPAEVSSNLARYDGIRFGHRAEGVKTLSELYG
                     RSRDEGFVTENKRRIMIGSYVLSSGYFDAYYQQAQKARTVLINEFNNLFSQYDLLLMP
                     TAPTPAFGIGENTADPVKMYLSDVMTVPMSLAGLPAISAPAGVSESGLPIGVQLVGPM
                     KADALVIATAGQMELQND"
     gene            2003..2428
                     /locus_tag="VLF64_00495"
     CDS             2003..2428
                     /locus_tag="VLF64_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90456.1"
                     /translation="MTSGPVVIFFLVVLLIVAIIAVLFLLVSSKKGPRLNVTRYQTQW
                     LAIENSVSRSNEASWQVAIMNADKLVDQALRESKYKGQTMGERMKAAEKTWRNPNHIW
                     GAHKIRNQLAHEVNMKLSYDITLRSLSAFKQALKDLGAI"
     gene            2428..3888
                     /gene="gatB"
                     /locus_tag="VLF64_00500"
     CDS             2428..3888
                     /gene="gatB"
                     /locus_tag="VLF64_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HSW90457.1"
                     /translation="MTDEVLAKYEMTIGIECHVQLATDTKLFSPADNDARDAEPNSKI
                     HPIDFGLPGMLPVLNRHAVDLAIRAGKALNAPIAHVSRFDRKHYFYPDLPKGYQTTQM
                     YQPIIEAGFVEAPLEDDSTVKVRIHHAHMEEDAGKLSHYNGYSLVDLNRAGTPLIEIV
                     SEPDIHSPAEARAYAAELHHLMTYAGVTFGNLFHGNMRFDVNLSVAPKGATELGRRAE
                     VKNLNSFRSVEKAAEYEFERQVAILERGERVAQETRGWNDDKGITTSQRSKEDAQDYR
                     YMPDADIPPIVLTDEEIMQIQADMPMLPGALRKILHVAGLDHSVTEVAINIGEPRVRD
                     FLVHIAAATNGAVRRRILNWLIIDVQGRISAGDDTWEGKQLLPEHLISLAEMAEKGEI
                     SSNSAVMVLNEVLINGDDPRMIAEGKNLLQIGDEGAIAAIVDEVLADPASSESIADIK
                     AGKGKAIGFLVGQIMKKSKGQANPAMAQKLIRERLS"
     gene            3890..4444
                     /locus_tag="VLF64_00505"
     CDS             3890..4444
                     /locus_tag="VLF64_00505"
                     /inference="COORDINATES: protein motif:HMM:NF018154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSW90458.1"
                     /translation="MAAPLLIVIQGAPGVGKTTILEKLRHDFTMPMLGKDEIKEFLFD
                     TIPQSDREFSRLQGGVSFDMLYVFARAFLGAGQPVIIEGAFMTKFASDAIQKILDDTG
                     AQYLEIFCYVDETVRQERFRARSESGNRHAAHMDGAATELPRDSHPYMALNLGDRIDV
                     DASEPLGGVYAQIVNEIKQRLTTG"
     gene            4532..5419
                     /locus_tag="VLF64_00510"
     CDS             4532..5419
                     /locus_tag="VLF64_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HSW90459.1"
                     /translation="MKRPTKAIITDAGFASRYLPITKTIPKAMLPFGSKPIMQLVVEE
                     CAEAGIREIIIVATPEGKPIYEDYFSNAVNHIRKQLRSQGKVDRYNKTVLEVLDLPKI
                     RVITQDPTLPYGNGSPIASASDYFGDDEAFIVMYSDDVVFGSSDAKKLVEAFDEYPDS
                     KAIIMAQEIPENEVDQYGIISLKDGDKLDHIVEKPEVGKAPSTLASYGRYLVTTEIFD
                     LLKPGAEGLDNELWTVDAITKLAATGDVRVVTTEGEWVTTGDPQNYFLAHMKYVMKNE
                     NYGETVRKAAQQAADNSIN"
     gene            5473..5721
                     /locus_tag="VLF64_00515"
     CDS             5473..5721
                     /locus_tag="VLF64_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90460.1"
                     /translation="MEWAQVLVIILSVFLAIFLLLGIALVVLLIKVTRQIKSVAGAAE
                     RTALKFESVAGQVAAISSPLAIAKTIADIVIKARSKKK"
     gene            5731..6018
                     /locus_tag="VLF64_00520"
     CDS             5731..6018
                     /locus_tag="VLF64_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HSW90461.1"
                     /translation="MSKGKFALGAIVGAAAGLVAGVLTAPKAGKETRADIKAKAGELK
                     ADADNKLKDAKKSGEKTMNEGRHMAEDYADRTKRALNSAKREFVTDDTKKK"
     gene            6090..6374
                     /locus_tag="VLF64_00525"
     CDS             6090..6374
                     /locus_tag="VLF64_00525"
                     /inference="COORDINATES: protein motif:HMM:NF040137.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5665 domain-containing protein"
                     /protein_id="HSW90462.1"
                     /translation="MKLWNKIKRQVREDNERGARESVLEDLFNDFNRNRFTIYKFNFF
                     RGIFFGLGSVLGGTLVVALLAGLLNLTGHLVPGIANFLNSVIDMMQRARR"
     gene            6432..>9861
                     /gene="dnaE"
                     /locus_tag="VLF64_00530"
     CDS             6432..>9861
                     /gene="dnaE"
                     /locus_tag="VLF64_00530"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HSW90463.1"
                     /translation="MGEKTVTPMTTASLRTSDFVHLHNHTHHSVLDGLTKINELAEKV
                     KEYGMEAAAVTDHGTMSGILDYYKAAKAAGIKPLLGIETYVAARTRFDRDPSKDKARY
                     HLTVIAMNNHGYQNLMQLSTRANLEGMYYKPRVDHELLEEYNEGLIVLSGCASGEVGE
                     SLRNDDYATALEHAKWYKSVFGDRYYLELQDHGHPDSATHWDVQAKINKGLKKIANEL
                     GIEMVVTCDGHYLTHDYLDAHEILLCVGTGSYLSDEKRMSLKEFELHLTDPKDIIKRW
                     GKEMPEVILNTKKIADRCDVEIELGKILIPKYPVPDGSSEHDYLERLVYTGVAVRYLG
                     MKREDAEALSPKEISDKLSDEVRERTEMEMGVMGTMGYEGYFLIVQDFINWGKSQGIV
                     FGPGRGSAAGSIIAYSLNITDLDPLKYGLLFERFLNPDRISMPDIDVDIQDTRRDEVI
                     QYCSDKYGANRVSNIVTFGKMAARAAVRDVARVLQVPYAEADRLSKMIPPPAQGRHIP
                     LARSVVEDHDLKAEYESNPSAKEVFDYAIQLEGTIRSHGVHACGVVIAPDDLVKFIPL
                     EQAQKGVVATQFPMGEVEELGLLKMDFLGLSNLTIINNAMRIIRKVYKEDVDLSKIPL
                     DDTATYELFQRGDTTGVFQLESAGMKRYLRGLKPTTFEDIIAMVALYRPGPMQFIDSF
                     IRRKHGQEKITYLHEGMRNSLENTYGILVYQEQFMQISKEWCGFTGGQADTLRKAVGK
                     KKIDLMRKVKVDFVEGAIKHGGATEEQAETFWAQLEEFANYCFNKSHAACYGLIAYWT
                     AYLKAHYPDAFMAAVMTSDHDDIDRLAIEITECKRMGLTVLNPDVNQSFLEFGVVPAA
                     KQIRFGMAAVKGVGTAAVEEIIRARELDGPFKSIEDFAKRVSTQKVNKKAYESLIKAG
                     AFDSLGSRSDLLFNLESILAFASKLQKEALSGQTDLFGALGGEQLMPAMELKPAPVKY
                     TDKEQLMWERELLGLYISSHPLDNYDTYFEEQTIPLRLLTPKIDGQKITIGGLVTTIR
                     TIVTKSGTKMAFVGIEDKTGEGEVVVFPKLYERLNGELKQDIILKITGNVSARDRDGN
                     MGDEAKIIADECNVVTDKELREYESHGTIMRAPTGQATVTRKRYPSKASADTGIA"
BASE COUNT         2729 a         2319 c         2477 g         2336 t
ORIGIN      
        1 cgtttttctc taacgtgtgg ccatctttgg ttgtaaagta gtacttgtac tgatgccctg
       61 gctcaacgtc cttaatggta attgaccaat aaccgtcttg ttcgctttcc atatcaagtg
      121 cgccaccagg agtaaaagta ccgccaatcg ctactttttc agcgaatgga gcccacaccc
      181 taaactcagc cttgccgtgc gataacgtaa cgccgaggtt tttcttaatc tgatggctca
      241 tgtacctatt taaccataag tggcatcaat ggccgctata tttcttgggc tttctttgat
      301 acaatatatc ccaatgagca caataaccac caatgatgtg cgacacctcg cacagctaag
      361 taatttgcag ctttcggacg acgaaatcac gagccttcag acagatcttg caaacatctt
      421 agattatatc aatcagctaa acgaaattga tacaactggc gtagagccga cctaccaagt
      481 aactggcctc gaaaatgtat ggcgtgacga caccgtagat acaggcaccg taacgcgcga
      541 aaatctacta aatttaaccc ccgaccaaac tgataacagt attaaggttc cgcaggtatt
      601 ggcatgagca ttcgtacctt agtagcccgg gttaataatg gtcagacaac ggcgcgtgcg
      661 ctcgttgagg aatcgcttca aaaagctcgt aaggccgagg aatatcatgc cctactaagt
      721 ctcaccgaag agcgcgcact cgctcgtgcc gacgaaattg atgcgcgtat taaaggaggc
      781 gaacaagttg gtctactcgc tggcataccg tttgtcgtta aagacaactt ccttgcgttt
      841 ggcgcgccaa cgactgcggc gagcaaaatg cttgaaaatt ttgaagcacc aatccaggcg
      901 acagttgtcg agaaactcga acaagccggt gccatctgta tcggtaagtc aaatctcgat
      961 gccttcgcgc acggcgggtc aacagaaaac tctgcttttg gcatgacgca taacgcaatc
     1021 gataaaacca aggttgctgg tggttcaagc ggtggttcgg cagttgtgac agcgcttgat
     1081 attgtgccat ttgcactcgg tagtgacacg ggcggctcaa ttcgtcagcc agcgagcttc
     1141 aacggtgtgt ttggtatgaa gccaacctac ggcatgagca gccgctatgg cgtagtagct
     1201 atggcgagca gtactgatgt aatgggctgt tttgcaaagt cagcagatga cgtcgcactt
     1261 atcgagagcg tgatggcagg ccaagatgca aaagatatga ccacgcttcc ggattacttc
     1321 agcccgctcg ctcgggtccc tgcaggccta aaggtcggtc tcgtaaaaga agcaatgacc
     1381 gacgatgtcg accctgaagt ccgcagggcc acagagcagt atgccgaaaa gctccgagct
     1441 gcaggccata cggttgatga agtaagtctc ccaagcgtca aacactcgct cgctatttac
     1501 tatattgtcg ttccggctga ggtttcgagt aacctggccc gttacgacgg cattcgtttt
     1561 ggccaccgtg ccgaaggtgt aaaaacctta agcgagctct acggacgcag tcgtgacgag
     1621 ggatttgtta ccgaaaataa gcgccgtatt atgattggta gctacgttct ttctagcggc
     1681 tatttcgatg cgtactatca gcaggcgcaa aaggcccgca ctgtattaat taacgaattt
     1741 aataacctgt ttagccaata cgacctccta cttatgccaa ccgcgccaac accggctttt
     1801 ggtatcggcg aaaacaccgc agacccagtc aagatgtatc tttctgatgt catgactgtg
     1861 ccaatgagct tggcgggatt gccagctatt agtgcacctg ccggtgtgag tgaaagcggc
     1921 cttccaattg gcgtgcagct tgtcggtcca atgaaagccg atgcacttgt tattgcaaca
     1981 gcaggtcaga tggagctgca gaatgactag cggcccagtg gtaattttct ttcttgtcgt
     2041 gcttttaatt gtcgcgatta ttgccgtgct ctttttgctg gtttcgtcca aaaaaggtcc
     2101 gagactcaac gtcactcggt atcaaacaca gtggcttgcc atcgaaaaca gcgtgtcgcg
     2161 cagtaatgag gcgagctggc aggtggcaat tatgaatgcc gataagctcg ttgaccaagc
     2221 cctgcgcgag agcaagtaca aaggtcaaac aatgggcgag cgcatgaaag cggcagagaa
     2281 aacctggcgt aatccaaatc acatatgggg tgcgcacaaa atccgcaacc aactagccca
     2341 tgaagtaaac atgaaactca gttacgatat cacccttcgc tcgctctctg cattcaagca
     2401 ggcgttgaag gatctaggag caatataatg acagatgaag tacttgcaaa atatgaaatg
     2461 acaatcggta ttgaatgtca cgtgcagctc gcaaccgata caaaattatt tagtccggcc
     2521 gacaatgatg ctcgcgatgc cgaaccaaac agcaagatcc acccaatcga cttcggactg
     2581 cctggcatgc tgccagtgct caatcgtcat gcagtcgacc ttgcgatacg tgccggcaaa
     2641 gcgctgaatg cgccaattgc acatgtaagt cgctttgatc gtaagcacta cttctaccca
     2701 gaccttccta agggctatca gaccacacaa atgtaccagc caatcatcga agctggtttt
     2761 gtagaagccc cgcttgaaga tgactctacg gtgaaagttc gtattcacca cgcacacatg
     2821 gaggaggatg ctggcaaact ctcgcactac aatggctata gccttgtcga tcttaaccgc
     2881 gcaggtactc cgcttatcga gattgtatct gagccggata tccatagccc agctgaagca
     2941 cgagcatatg ccgcggagct tcatcacctg atgacctatg ctggcgtaac atttggtaat
     3001 ctgttccatg gcaacatgcg ttttgatgta aacctctcag tagcaccaaa gggcgcgact
     3061 gagcttggcc gccgggcaga agtaaagaac ctcaatagct tccgtagcgt cgaaaaagca
     3121 gccgaatacg agtttgagcg tcaggttgcg attctagagc gtggcgaacg ggtcgctcag
     3181 gaaacccgcg gctggaatga tgataaaggc attacaacaa gccaacgctc taaagaagac
     3241 gcccaggact accgttacat gccagacgcc gacattccac caattgtgct aaccgatgaa
     3301 gagatcatgc agattcaagc agatatgcca atgctgccag gcgcgctccg caaaatcttg
     3361 catgttgcag gtcttgatca ttcagttaca gaagtagcaa tcaatatcgg cgaaccgcgc
     3421 gtgcgcgact ttcttgtgca cattgctgcg gctaccaatg gcgctgtgcg tcggcgtatc
     3481 cttaactggc ttatcattga cgtccagggg cgcattagcg cgggcgatga cacctgggag
     3541 ggcaaacagc tgctcccaga acacctcatt tcgctagctg aaatggcaga aaagggtgaa
     3601 atcagcagta actctgctgt aatggtactg aatgaagtac tcataaatgg cgacgatcca
     3661 cggatgatcg ccgaaggcaa aaatctccta cagataggcg atgagggtgc aatcgcagca
     3721 attgtcgatg aagtcctcgc cgatcctgct agtagcgaga gtatcgccga cattaaagcg
     3781 ggtaagggca aagcgattgg cttcctggtt ggccagatta tgaaaaaatc caaaggccag
     3841 gcaaatcctg caatggcaca aaaacttata cgagagcggc ttagctagta tggccgctcc
     3901 tttacttatt gttattcaag gtgctccagg ggttggtaaa acaacgatcc tagagaaatt
     3961 acgccatgat ttcacaatgc caatgctcgg taaagatgag atcaaagagt tcttatttga
     4021 caccattccg cagtcagacc gtgagttctc acgattacaa ggcggcgttt catttgacat
     4081 gttgtacgta ttcgctcgcg ccttcttagg cgctggccag cccgtcatta tagagggcgc
     4141 atttatgaca aagttcgcga gtgatgcaat tcagaagatt cttgatgata ccggcgcgca
     4201 gtatcttgaa atattttgct acgtcgatga aacggtgcgc caagaaaggt tcagggcacg
     4261 ttctgaaagc ggcaatcgac atgctgcaca tatggacggg gcggcgacag aattgccgcg
     4321 tgatagccat ccatatatgg cgctgaacct cggtgatcgg atcgacgtag atgcatccga
     4381 gcctctcggg ggcgtatacg cacagattgt aaatgaaata aaacagcgac ttacgacagg
     4441 gtagggctaa ccgtagccga ggtgccaaac gtggccgctt ggcacggtgg ccttctatga
     4501 ttatactagt gaaaatgaaa ggggctaaca aatgaaacga ccaactaaag caattattac
     4561 tgacgcagga tttgcgagcc gttacctacc tatcacaaag acaatcccaa aggcaatgct
     4621 gccatttggt agcaagccaa tcatgcagct tgttgttgaa gaatgcgccg aggcaggcat
     4681 tcgtgaaatt attattgtgg ctactcctga gggcaagcca atctacgaag actattttag
     4741 caatgccgtg aatcatatcc gtaaacagct gaggtctcag ggcaaggttg accgttacaa
     4801 caagacagta cttgaggtac tggaccttcc taaaatccgc gttattaccc aagatccaac
     4861 gctcccgtat ggtaatggtt caccaattgc gagtgccagt gactacttcg gcgacgacga
     4921 ggcgtttatc gttatgtaca gcgacgatgt tgtatttggc agttcggacg ccaagaaact
     4981 tgtcgaggca tttgacgagt atcccgattc aaaagccatc atcatggcac aggaaatccc
     5041 cgaaaacgaa gtcgatcagt atggcattat ctcgctgaaa gacggcgaca agctcgacca
     5101 catcgtcgaa aaaccagaag ttggcaaggc gccatcaaca cttgcgtcgt atggtcgcta
     5161 tcttgtaacc accgagatct tcgatctact taagccaggt gccgaaggac tcgataatga
     5221 actttggact gttgacgcaa tcacaaagct cgctgctacg ggtgacgttc gtgttgttac
     5281 gaccgagggc gaatgggtaa caacaggtga cccgcaaaac tatttcttag cacacatgaa
     5341 atatgtcatg aaaaacgaaa actacggtga aaccgtgcgt aaggcagctc agcaagccgc
     5401 cgataacagt ataaactaag aatagtggta aaataagata tagacactac tcagcgaaag
     5461 gaatacgtaa ttatggaatg ggcacaagta ctggtaatta ttctgtcggt attcttggca
     5521 attttcttat tgctcggcat cgctcttgtc gtgctcctta tcaaggtgac acgccaaatt
     5581 aaatcagttg caggtgcagc tgagcgtaca gcattaaagt ttgagagtgt tgcagggcag
     5641 gttgcagcga tttcatcgcc acttgctatc gcaaaaacaa ttgccgatat tgtcatcaag
     5701 gcaagatcta agaagaaata aggaggggat atgtcaaagg gaaaattcgc gcttggcgca
     5761 atcgttgggg cagcggccgg tcttgttgct ggtgtcctta cggcaccaaa agcaggcaaa
     5821 gagacgcgtg ccgatatcaa ggctaaggcc ggagagctta aggctgatgc tgataataag
     5881 ctgaaagatg caaaaaagag tggtgagaag acgatgaacg aaggtcgaca catggccgaa
     5941 gattatgctg atcgcacaaa gcgcgcactg aatagcgcga aacgtgagtt cgtcacagac
     6001 gacacgaaaa aaaagtaagg cttgcagctg aatacacaat cgtggcatac tgaacatagc
     6061 cagtatgcca ttttgtttag gggtaaataa tgaaattatg gaataaaatt aagcgccagg
     6121 tacgcgaaga taacgagcgt ggcgcccgcg aatcagtact cgaagatcta tttaacgact
     6181 ttaatcgtaa tcggtttaca atttataagt ttaatttctt ccgtggtata ttttttggtc
     6241 tcgggtcggt gctgggtggg acgcttgttg tagcgcttct tgcgggctta ctaaacctta
     6301 ccggccatct cgttccaggc atcgccaatt ttcttaacag tgtcattgat atgatgcagc
     6361 gcgctcgtag ataacatagg cttttttacg acatttttct cttccctgga gtggtatact
     6421 agatagtagc tatgggggaa aaaacggtaa caccaatgac gactgcgtcg ctcagaacga
     6481 gcgattttgt acatttgcac aatcatacgc atcactctgt acttgatggt ctgacaaaaa
     6541 tcaatgaact tgccgaaaaa gtaaaagaat atggcatgga agcggccgca gtaaccgacc
     6601 atggtacgat gagtggcatc ctcgattact ataaggctgc taaagcggct ggcatcaaac
     6661 cgctccttgg tatcgaaacg tacgttgcag cgcgtacgcg ctttgaccgc gacccaagta
     6721 aagataaggc ccgttaccac ttaacggtga tagccatgaa caaccatggc tatcaaaacc
     6781 tcatgcagct ttcgacacgg gctaatcttg agggtatgta ttacaaaccg cgcgtcgacc
     6841 acgaattact tgaagagtac aatgagggac tgattgttct atcaggttgt gcgagcggtg
     6901 aagttggtga gagccttcgc aatgatgatt atgccacagc gctcgagcat gccaagtggt
     6961 ataaaagcgt gttcggtgac cgctactacc ttgaactcca ggatcatggc catccagaca
     7021 gtgcaactca ttgggatgtc caggctaaga ttaacaaagg tcttaagaaa attgccaatg
     7081 aacttggtat tgagatggtt gtgacctgcg acggccacta ccttacccat gattatttgg
     7141 acgcacatga aatcttgttg tgtgttggca cgggttcata tcttagcgat gaaaagcgta
     7201 tgagcttgaa ggagttcgaa ctccacctca ctgatccaaa agacatcata aagcgctggg
     7261 gaaaagaaat gccagaagta atcttgaaca cgaaaaagat tgctgatcgc tgtgatgttg
     7321 aaattgagct tggtaaaatt ctcattccaa agtacccagt accagatggt tcaagtgagc
     7381 acgactatct tgagcgactt gtgtacactg gtgtcgcagt gcgatatctt ggaatgaagc
     7441 gcgaagatgc cgaggcgctc tcgccaaaag aaatctcaga taaattaagt gacgaagtcc
     7501 gtgaacgcac cgaaatggaa atgggcgtca tgggcacgat gggctacgag ggttacttct
     7561 tgattgtcca ggactttatc aactggggta aatcacaagg tattgtcttt gggccaggcc
     7621 gcggtagtgc agcggggagc atcatcgctt attcccttaa catcaccgat cttgacccac
     7681 tcaaatacgg tctactattt gaacgtttcc tgaatccaga ccgtattagc atgcccgata
     7741 tcgatgtcga tattcaggat acgcgtcgag atgaagtgat ccagtactgc tcggataaat
     7801 atggtgcgaa tcgtgtatca aacatcgtga catttggtaa aatggctgct cgcgctgcag
     7861 tgcgtgatgt cgctcgtgta ctgcaggtac cgtacgcaga ggccgaccga ctcagcaaaa
     7921 tgattccacc acctgctcag ggccgtcata taccactcgc aagatctgtt gttgaggatc
     7981 acgacctaaa agctgagtac gaatcaaatc catccgcgaa agaagtcttc gattatgcaa
     8041 ttcagcttga aggtacgatt cgctcgcacg gcgtccatgc ttgtggcgtg gtgattgccc
     8101 cagacgacct tgtgaagttt atccccctcg agcaggccca aaaaggcgtt gtagcaacgc
     8161 agttcccaat gggcgaggtt gaagaactag gtcttctcaa gatggatttc ttgggacttt
     8221 ctaacctcac gattatcaac aatgctatgc gtatcattcg caaggtgtat aaagaagacg
     8281 tagatctttc taagatcccc cttgatgata cggctaccta cgaactcttc cagcgtggcg
     8341 acacgacagg cgtattccag cttgagtctg ccggtatgaa gcgttacttg cgtggtctga
     8401 agccaaccac ctttgaggat atcatcgcaa tggttgcctt gtatcgcccg gggccgatgc
     8461 agttcattga tagctttatt cggcgtaagc atggccagga aaaaatcacc tacctacatg
     8521 aaggcatgcg taactcgctc gaaaacacct atggaatctt ggtatatcag gaacagttca
     8581 tgcagatttc taaggaatgg tgtggattta ccggtggtca ggcggacacg ctccgtaagg
     8641 cggtgggtaa gaagaagatt gaccttatgc gcaaggtgaa ggttgatttc gtcgaaggcg
     8701 cgattaagca tgggggggct actgaagagc aggccgaaac tttttgggcg cagctcgagg
     8761 aattcgcaaa ctactgtttc aacaagagcc atgcggcgtg ttatggcttg atcgcctact
     8821 ggacagccta tctaaaggca cactatccag acgcctttat ggcagcggtt atgactagcg
     8881 atcacgatga catcgatcgc ctcgcaatcg agatcaccga atgtaagcgc atggggctta
     8941 cagtgcttaa cccagacgtt aatcagtcat tcctcgaatt tggtgtggtt cctgcggcta
     9001 aacagattcg ctttggcatg gcagccgtga agggcgtcgg cacggccgca gtcgaagaaa
     9061 ttatccgtgc acgtgaactc gatggtccat ttaaaagcat agaggacttc gccaagcgcg
     9121 ttagtacgca aaaagtaaac aagaaggctt acgagtcact cattaaagct ggcgcattcg
     9181 atagccttgg tagtcgctcc gatcttctgt tcaaccttga atcaatcctt gcgtttgcaa
     9241 gcaaactgca aaaagaagcg ctcagcggcc aaacggatct ctttggcgcc ctcggtggtg
     9301 agcaactcat gccagcaatg gagctcaaac ccgcgccagt caaatacact gacaaggaac
     9361 agcttatgtg ggagcgtgag ctcctcggac tgtacatttc ttctcatccg cttgataact
     9421 acgatacgta ctttgaagag cagacaattc cgcttcgctt gcttacacca aaaatcgatg
     9481 gtcaaaagat cacgattggc ggtcttgtca caacaattcg caccattgtt acaaaatcag
     9541 gcacaaagat ggccttcgta ggtatcgagg acaaaacggg tgaaggtgag gtggtagtct
     9601 ttcctaaatt atatgaacgt ctcaatggtg agctcaagca agatatcatc ctcaagatta
     9661 cgggtaatgt tagcgcacgc gaccgcgacg gcaacatggg cgatgaagca aagataattg
     9721 ctgatgaatg taatgtcgtg acagataaag agcttcggga atacgaaagc cacggcacaa
     9781 taatgcgcgc acctactggc caagccacgg ttacgcgtaa gcgctatcca tcgaaggcat
     9841 cagcagatac gggcattgca a
//
LOCUS       DATIIX010000019         8587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_531961, whole genome shotgun sequence.
ACCESSION   DATIIX010000019 DATIIX010000000
VERSION     DATIIX010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 8587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8587
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531961"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="VLF64_00535"
     CDS             <1..1016
                     /locus_tag="VLF64_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90464.1"
                     /translation="AILVGYAIVACVQATAAKADGGEFSLQVSPSPLTATLKPGQTSS
                     QTLQIRNDGTQTENLKILPRSFTISNSNRLDLDDAHVPEAASWISFSAPTFTVAPGQI
                     FTDTIKFTVPKDAGFSYSFALIITRQDTPQDATTGRLLKGSVAVFTLINIDRPGATRQ
                     LQIAKFTPSQPVYEYLPATLNITFKNTGNTIVQPAGNIFIQRNENDTSSLDTLPVNQN
                     ESYILPGSTRTLPVAWTDGFQVLKPVTQANGSIGSQLTWNWGNLSHLRIGQYTAKVVA
                     IYNDGTRDVPIIGEVSFWVIPWKLLLGALVVVCLIGIGVWSIIRPIIQAARRKKKDKH
                     VHF"
     gene            1247..1948
                     /locus_tag="VLF64_00540"
     CDS             1247..1948
                     /locus_tag="VLF64_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90465.1"
                     /translation="MYPQRLPLVNSDDGTWGNIIRQYLMAQHYNDDTNNPLNGSHQKV
                     TIVAGTATAGTAPLKFTSGTVLATPEAGAVEFNTDALYFTITTGTVRKRVAIYDDSAG
                     ANGDTYYRDSSGNFVRRAIGTTGQYMRVSGGVPTWVDPTFATSSKTANYTVSSSDTVV
                     FADATSGNITITLPLASSLSGYRFYVKRVDGAANNCSVACSGVDTVDGQSSISIVAQY
                     TSLTIVSNGSAWYII"
     gene            1953..3305
                     /locus_tag="VLF64_00545"
     CDS             1953..3305
                     /locus_tag="VLF64_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90466.1"
                     /translation="MTYIPPVNGGRINVTIGGNTTGTGALMSTGTATLVGGNNITLSQ
                     NGNALTIVGGAGGGGGVALANSQTTYNSGTANFVASGALTIVSATGQSFNFSVPQTSN
                     LSATGAVSISSNAGTISIGAPAFSAGISNIGNALGTTGTASNRVIFAGGNNVTLSQST
                     NAAGNTITIQAGGGGMALANSQTTFTSGTANFIEGGGAITIASTTGQSFKVSVPQISS
                     LSATGAVSISTNGGTISIGVPAATLSRFEYPEGLFTSLGAIGQGSLSINHMYVPFYVT
                     GTAAKIGGSLSAATNTSATTASANISLWMGIYTLNGSTLSLASSGSANNGFQFSQSAS
                     STGNTSINSMRQMTVPMNINISPGEYWMAAVISSATTYTSAGFTMYGNNQVANAASAA
                     ILSPIGATAARDVMLLQGIYTAATSAGPTSIAGTQINNTAASIVQRANFYNVIFNATY
                     "
     gene            3312..3947
                     /locus_tag="VLF64_00550"
     CDS             3312..3947
                     /locus_tag="VLF64_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90467.1"
                     /translation="MPITFVQGNANGDSSSGTVTTCAFTSNVTAGNLLVVAVSFASAI
                     ETASVTDSLGNTYSTAVGPTTSNTIASRVYTFYTVTGLSGANTVTITNSNLVLFRRVS
                     VHEYSGVNTLEVATGTNADTGAPDSGPVTTTHANALIFGWGISNNGTTSAGTGFTLRE
                     TALSESTEDMIVSSTGTYDAIYPSDGSAWICQVAAFYYSAPAAITVAWLTA"
     gene            3994..4719
                     /locus_tag="VLF64_00555"
     CDS             3994..4719
                     /locus_tag="VLF64_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90468.1"
                     /translation="MGIPQILVPDNAGVHNTSDYTSRLKKEKTYRDLSTIIVCPTRGM
                     IPARIVQSWQGMIRPMNQKVLGPIFMEGMEVGEAYNQVIKMILENPDLANFKFMLTIE
                     EDNAPPVDGLMKLYESIDDYDAVGGLYWTKGEAGQPMCYGNPSTMPLNFIPQIPTPGA
                     VTPCNGLGMGFTLFRLNMFKDKRLTYGEWFKTKQEVAVDGSVQVYTQDLWFFQNAKEL
                     GYKFACDAQVRVGHYDATNQIMW"
     gene            4729..5325
                     /locus_tag="VLF64_00560"
     CDS             4729..5325
                     /locus_tag="VLF64_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90469.1"
                     /translation="MVKKDQKKDKPLRLSLACGDAKPDGFKGVDIVKTESADYVFDLL
                     SFPWTQIADNSVDEIECSNFVEHIPHGDSRNDLFFQFFDEIHRILKPAEFNPDNPNIP
                     LKGFARITCPYYSSMRASQDPTHNRAISEASFLYLNKQWRADNKLSHYPVACDFDFSY
                     GYILSPEWQNRNQETQLFAIQHYINVVSDIQVQLVKRG"
     gene            5328..6206
                     /locus_tag="VLF64_00565"
     CDS             5328..6206
                     /locus_tag="VLF64_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90470.1"
                     /translation="MSKWRRSISVAAAIIVLLISNTPPVAATNNQTQGILITPARQYI
                     TAAAGASQTYQLSVANITNKPVDISFSVKQFRVADYTYDYTFLPVKENWITFSTPQIH
                     LASGDSKTISYVVGVPPGAVPGGHYFTLFASASLYSGAVPIDVQVTSVMYVTVSGSLI
                     KTSSIKHSSIPTISFGGDIPFSIDVHNTGNTHFFVYASGKLSGWTAKPTIDQGAHIIL
                     PGTTRTVNGVIAAPLLPGIYTAKYGYKVDDGRQVNTSQTIVYLPPWAIIIPIAIIWIT
                     WTIIVRIKRRVIRFSR"
     gene            complement(6187..6759)
                     /locus_tag="VLF64_00570"
     CDS             complement(6187..6759)
                     /locus_tag="VLF64_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90471.1"
                     /translation="MVNSIIKDVRYRLTFASMFLLVFALLSSTIAYATPFGKGVFGAQ
                     VPFGSANSISMNLGSNVAINLSPSGSTFVGTGAHTVTVTTTDAVGYTLYVHTTGSANM
                     TSTGGATIAASTNSTAGALSNNTWGYNTTGSTTNFIGMTTQDSIVKDASGPYETGDPT
                     TITYGAVVDNVQPAGSYSIAITYTAIAKNE"
     gene            6937..7251
                     /locus_tag="VLF64_00575"
     CDS             6937..7251
                     /locus_tag="VLF64_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90472.1"
                     /translation="MTTKRKRSTVYEPSGNYPRRVSATITTKDEFFNLPVMEDSPTSD
                     VFFLGQVHFCTDQARTDQECSFLAIEERRGGRPECPRHLRQSMSWANLYSVDDKVYRY
                     RY"
     gene            7703..7858
                     /locus_tag="VLF64_00580"
     CDS             7703..7858
                     /locus_tag="VLF64_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90473.1"
                     /translation="MLVTEARQRASGQDYMPAGLAMAAETVRPAWMDLAREVSEQVLG
                     HAGGAPA"
     gene            complement(8000..8425)
                     /locus_tag="VLF64_00585"
     CDS             complement(8000..8425)
                     /locus_tag="VLF64_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90474.1"
                     /translation="MKVNFERFRATDGIELQGWHSEPVDQAGTDLAALHVHGFAGDGH
                     TNTFLDSLHQAYPDAESSFFSFDTRGSGIIGWSNKNGEPVTIGSCYEVFPDGIHDIQG
                     AARVVEARGARRLIVEGHSLGASKVIDYCLNRDDDALPI"
BASE COUNT         2351 a         2064 c         2048 g         2124 t
ORIGIN      
        1 ttgcaatact tgtggggtac gcaattgttg catgcgtaca ggcaacagca gctaaagctg
       61 acggtgggga gttttcgcta caggtatcac catcaccgct tactgcaacg ctaaagccag
      121 gtcaaacatc gtcgcaaaca ttgcaaatac gcaatgacgg cactcaaact gaaaatctaa
      181 aaattctgcc gcgaagtttt acgatcagca attcaaatcg attagacctt gacgatgcgc
      241 atgtacccga agctgcaagc tggatatcgt ttagtgcacc tacatttacg gtcgcgccag
      301 gacagatatt taccgacacg attaaattta ccgtaccaaa ggatgccggg tttagctatt
      361 cattcgcgct tattattacg cgccaagata cgccgcagga tgccacgacc ggccggttac
      421 taaaaggatc ggtggcggta tttacgttga tcaatattga tcgaccaggt gcaactagac
      481 aattgcaaat tgcaaagttt actccatcgc aaccagtgta tgaatacctg ccagcaacct
      541 tgaatattac attcaaaaat accggcaaca cgattgtaca acctgctggt aatatattta
      601 ttcagcgtaa cgaaaacgac acatcctcac tagatacgct gccagtaaac caaaatgaga
      661 gttatatttt gccaggtagc acacgcacat tacccgttgc gtggaccgat gggtttcaag
      721 tgctgaagcc agtcacacag gccaatggta gcattggtag ccaattgacc tggaattggg
      781 gtaatctgtc acatctacgg attggccagt atactgcaaa agttgtggct atctataatg
      841 acggtacacg tgatgtacca ataattggtg aagtaagttt ttgggtaatt ccatggaaat
      901 tgcttttggg cgcactggtt gtggtgtgcc tgatcggtat aggtgtgtgg tcgataattc
      961 gtccgattat tcaggcagcc agacgcaaga agaaggataa gcacgttcat ttttaaaaga
     1021 atcggtgcga gggagtgtgg acaatggaga gtattgaaaa gttattacaa aaggagatgg
     1081 accgcaaaga attcctgcaa ttttctggta gcattatttt ggtaatactt ggcctaggca
     1141 atttggtctc gtttgtgcgc aaatacactg actcacacag tgcttcatcg gcgcctgtga
     1201 cgacaaataa ccaacatggc tttgggtcca gaaagtttgg tgcttaatgt acccacaacg
     1261 acttccgctt gtcaacagcg acgacggtac atggggtaac atcatccgcc agtacctgat
     1321 ggcgcagcat tataacgatg acaccaacaa ccccctaaac ggtagccacc aaaaagtaac
     1381 aattgttgcc ggcacagcaa cagctggtac agcaccatta aaattcacat ccggtacggt
     1441 attagcgacg ccagaggccg gggcggtcga attcaatacc gatgcactat attttacgat
     1501 taccacaggt acagtgcgca agagagttgc aatatacgat gactcggcag gcgcaaacgg
     1561 tgacacgtac taccgtgata gttcgggtaa ttttgtccgc agggcaatcg gtacgacggg
     1621 tcagtatatg cgcgtatctg gcggtgttcc tacatgggtc gatccaacgt tcgcgactag
     1681 ttcaaagaca gccaattaca ccgtgtcctc atctgacacg gtagtatttg cagatgccac
     1741 aagtggtaac ataacaatta cattgccact tgcatcgtca ctatcaggat acaggtttta
     1801 tgttaaacga gttgatgggg cggccaataa ctgttcggtt gcatgttcag gcgtagatac
     1861 ggttgatggt cagtcgtcaa tatcaattgt agcgcagtat acatcattaa cgatcgtcag
     1921 taatggatca gcttggtata taatataact atatgacata tattcctcca gtcaacggcg
     1981 ggcgcattaa cgtcacgatt ggcggcaata cgaccggaac gggcgcgctg atgtctacgg
     2041 ggacagcaac gttggttggt ggtaataata ttacattatc gcaaaacggt aatgctctta
     2101 cgattgtcgg tggtgcaggc ggtggcggag gtgtggcgct tgccaactcg caaaccacct
     2161 ataatagtgg tacggctaac tttgtggcca gtggtgcgct aacaattgtg tcggcaacgg
     2221 ggcagtcgtt taacttttcg gtaccgcaga cgtcaaactt gtcggcaaca ggtgcagtta
     2281 gtatatcttc gaacgccggc acgataagca ttggcgcgcc agcgttctct gctgggataa
     2341 gtaatattgg caatgcacta ggtacaacag gtaccgcgtc taaccgtgtt atttttgcag
     2401 gtggcaacaa cgtgacttta agtcagtcaa ccaacgcagc tggtaacacg atcactattc
     2461 aggcaggcgg tgggggtatg gcgcttgcca actcgcagac gacgtttacg agtgggacgg
     2521 cgaattttat cgaaggcggc ggagctataa ccattgcctc cacgactggt caatccttta
     2581 aagtttctgt gccccaaatc tcaagtctgt cagccactgg cgcggtcagt atatcgacaa
     2641 atggcggtac tattagcatt ggcgtgccag ccgcgacact atcacgtttt gaatatccag
     2701 aaggcttgtt tacctcacta ggagcaatcg gccaaggttc gctttcgatc aaccacatgt
     2761 atgtcccatt ctatgtcacg ggcacagcgg ctaaaattgg tggttcactc tctgccgcaa
     2821 ccaatacctc ggccacgact gcatcggcca acatatcgct ctggatgggc atttacaccc
     2881 tcaatggctc aaccttatct cttgcaagct ctggcagtgc caataatggc tttcaattct
     2941 cgcagtctgc cagcagcact ggtaatacat ccatcaactc tatgcgtcaa atgaccgtac
     3001 cgatgaacat caatatcagt ccaggcgaat attggatggc agctgtcatc tccagcgcga
     3061 caacatatac cagtgccggg tttacgatgt atggcaacaa ccaggttgca aacgctgctt
     3121 cagctgccat tctgtcacct attggtgcga cggcagcacg tgatgtcatg ctcctccagg
     3181 gtatctacac agccgcaaca agtgccggac cgacatcgat agcaggcaca cagatcaaca
     3241 acaccgcagc cagcatcgtc caacgcgcca atttctataa cgtcatattt aatgcaacat
     3301 actaaatcac tatgccaata acatttgtcc aaggaaatgc aaacggcgac tcgagcagcg
     3361 gaaccgtcac tacctgcgcg tttacctcaa acgtaactgc gggtaatttg ctggtggtag
     3421 cagtgagttt tgcatccgcc attgagacgg catcggtaac ggacagcctc ggcaatacct
     3481 acagtactgc cgtcggaccc accaccagca acaccattgc ctccagggtc tatactttct
     3541 acaccgttac aggattaagc ggcgccaaca ccgttacaat caccaattct aatctagtcc
     3601 ttttccgtcg cgtatcagtg cacgaatact ccggcgtcaa tacactcgaa gttgccaccg
     3661 gcacaaacgc cgataccggg gcaccagaca gtggacccgt cacaaccacc catgcaaacg
     3721 cgcttatctt tggctggggc attagcaaca acggcacaac gtcagcgggc acgggtttta
     3781 cgctgcgtga gacggcactt tccgaatcaa ccgaggacat gattgtcagc agcaccggaa
     3841 catacgacgc aatttacccc agtgacggca gtgcgtggat ttgccaagtg gctgcttttt
     3901 actactccgc ccccgcggcc attaccgtgg cttggctaac agcctaggtc actttcgaga
     3961 tgataagcgt tagggtggta taatatataa attatgggaa ttccacagat actcgttccg
     4021 gataatgctg gcgttcacaa tactagtgat tacacgtcac gcctcaaaaa agaaaaaacc
     4081 taccgcgacc tcagcaccat catcgtttgt cccacacgag gtatgattcc ggcaaggatt
     4141 gtgcaaagct ggcagggtat gatacgcccc atgaatcaaa aagtgcttgg cccaatattt
     4201 atggagggca tggaggttgg cgaggcgtac aatcaggtga tcaaaatgat tttggagaat
     4261 ccagatcttg ccaattttaa gttcatgctt acaatcgaag aagataacgc gccgcctgtt
     4321 gatggtctga tgaaattata cgaatcgatt gacgattacg atgctgtggg tggtttgtac
     4381 tggacaaagg gcgaagctgg ccagcctatg tgctacggta accctagtac aatgccactt
     4441 aactttattc cacagattcc aaccccaggc gcggttacgc cgtgcaacgg attgggcatg
     4501 ggcttcacgc tgtttcgatt gaatatgttt aaggataaaa gattgacgta tggtgagtgg
     4561 tttaaaacca agcaagaagt tgcggtcgac ggtagcgtgc aagtgtacac tcaggatctt
     4621 tggttttttc aaaatgccaa ggaattagga tataaatttg cttgtgatgc gcaagtgcga
     4681 gtggggcact acgatgcgac aaatcagata atgtggtagg aggtgattat ggtaaaaaaa
     4741 gatcaaaaaa aggacaaacc attgcgactt tcgttagcgt gtggcgatgc taaaccagac
     4801 ggatttaaag gtgtcgatat cgtcaaaaca gaatccgctg actatgtgtt tgatttactt
     4861 agctttccat ggacgcaaat tgccgataat tcagttgacg aaatcgaatg ctctaacttt
     4921 gtcgagcata ttccgcacgg cgatagtcgc aatgacctat tttttcagtt ttttgacgaa
     4981 attcaccgta ttttaaaacc agccgaattt aacccagaca atccaaacat tccgctgaaa
     5041 ggctttgcga gaattacctg tccctactat tccagtatgc gcgcatcgca agacccaacc
     5101 cacaaccgtg ctatatccga ggcgagtttt ttgtacctta acaaacaatg gcgcgccgac
     5161 aacaaactga gtcactaccc ggtagcctgc gattttgatt ttagctacgg atatatcttg
     5221 tcaccggaat ggcaaaaccg caatcaagaa acacagctat tcgccatcca acactacatc
     5281 aatgttgtgt cagatattca ggtgcagcta gtaaaacggg gctaggcatg agcaagtggc
     5341 ggaggagtat cagtgtagcg gcggcaatta ttgtgctgct aataagtaat acaccaccag
     5401 ttgctgcgac caacaaccaa acccaaggca tactcataac tcccgcgcgg cagtatatta
     5461 ctgcagcggc aggcgcctcc caaacatatc aactatcagt agcaaacatc actaacaaac
     5521 cagttgatat tagcttttcg gttaaacagt ttagggttgc cgactatact tatgattata
     5581 cgtttttacc ggttaaagaa aactggataa catttagtac gccgcaaatc catttggcat
     5641 caggggacag taaaacaata tcatacgttg taggagtgcc gcctggtgca gtaccgggcg
     5701 gacattattt tacactgttt gcatctgcct cgctgtattc tggtgcagta ccgatagatg
     5761 tacaggtgac atcggttatg tatgtaacgg tcagtggtag cctgatcaaa acaagcagca
     5821 ttaaacattc atcaattcca actatttcgt ttggcggcga tattcctttt tcaatcgacg
     5881 tccataatac cggtaatact cactttttcg tatatgcatc aggtaaatta agtggctgga
     5941 ccgccaagcc gacaattgat caaggcgcgc atattattct gcccggcaca acccgaacgg
     6001 taaacggtgt cattgctgca ccactcttgc ccggaatcta taccgccaag tacggataca
     6061 aagttgatga tgggcgccaa gtgaatacat cgcaaacgat tgtgtattta ccaccgtggg
     6121 caattattat tccaattgcc atcatatgga taacatggac gataatcgta aggattaaac
     6181 gacgtgttat tcgtttttcg cgatagctgt gtaggtaata gcgatcgaat aactaccggc
     6241 tggttgaacg ttgtcaacga ccgcaccata agtaatggta gtcggatcac ctgtttcata
     6301 tgggccactg gcatctttta ctatcgaatc ttgtgtcgtc attcctataa aatttgttgt
     6361 cgatccggtt gtgttatacc cccatgtatt gttggatagt gcacccgcgg tcgaattggt
     6421 actggcggca atggttgccc cgccagtact tgtcatgtta gcgctaccgg tcgtgtgaac
     6481 gtacagcgtg taacctacag catcggtcgt tgtgacagtt actgtatgcg caccagtgcc
     6541 aacgaatgtt gatccgctag gcgataaatt aatggcaacg ttacttccca aattcataga
     6601 tatactattg gcagatccaa atggtacctg cgcgccaaat acacctttgc caaacggtgt
     6661 cgcataagcg attgtcgatg acaatagcgc aaacacaagc aaaaacatac tcgcaaatgt
     6721 tagccgatag cgcacgtctt tgataatact atttaccatc tgatctaagt ataacaaaag
     6781 taaaaaagtc gacattgcct gaaggattat aaaacaggtt tgcatactca ctccccctaa
     6841 gcggtttgtg ggggctgtta gaatttggca tatctcggtt tatagatatt cgaccttgta
     6901 tgtttagggt gcacctttta tgggattctg gagcaattga caaccaagcg taaaagatct
     6961 acagtatacg agccaagtgg caactatccg aggagggtta gcgcgacaat cacgaccaag
     7021 gacgagttct tcaatctgcc ggtaatggag gactctccta cgagtgatgt gttcttcctt
     7081 ggccaggtgc acttttgcac cgatcaggct cgcaccgacc aggagtgctc gttcctcgcc
     7141 atcgaggagc gccgcggcgg aaggccggaa tgcccgagac acctgcgaca gtcaatgtct
     7201 tgggctaacc tctatagtgt tgatgacaag gtataccgtt atcgctatta agaggaatcc
     7261 ggcctgtaat cccacccaaa gctggtatag ccaagaactg gctgcctgtc ttcaccccgc
     7321 gtgtcccaac tgggacatgg cggggtgtct tcataattca gttacaaaat taccgggagt
     7381 tcaatcagat gaagctcgta ttgtgactag agatatactg cccctgactt atacttatct
     7441 cattgaaaaa tatagactta tatgttatcg tcttgcatag gccacaagct gagaagaggt
     7501 gagtgctgcg gcaaagaaga cagtcgtaca gttcgccccc aagcgcgaca cgatcatatt
     7561 tagcgtcagg aatctgggtt gcagtgcaga cgcgctgcaa cggagagccc gacgctacgc
     7621 cgaggagagt gggcaccagg tcgttgtgac gaaacaccgt cgcgacacca taaaggtgac
     7681 cctctccggg aaagcgatcg ccatgctggt cacggaggcg cggcagcgtg catccggaca
     7741 ggactacatg ccggctggcc tggctatggc ggcagaaacc gtcaggccag catggatgga
     7801 ccttgcgcgt gaggtgagcg agcaagtgct cggtcacgcg ggcggggcgc cagcatagcg
     7861 ccccgcccct atttttcaat ttatctttta ctttgaacta actatgctga aggcgaaaag
     7921 cgtcatccat tgcaacgatt tcttcgaaca agtcaaagtt accatcctct aatactcgta
     7981 gtatgcgatt atttccagat cagattggca aagcgtcgtc gtcgcgatta aggcaatagt
     8041 cgattacttt acttgctcca agagaatgac cctctacaat aagccgtctc gcgccgcgtg
     8101 cctctactac ccgtgcagca ccctgaatat cgtgaatgcc atccggaaaa acctcataac
     8161 agctgccaat cgtgaccggt tcaccgttct tattcgacca acctataatg ccactgccac
     8221 gagtgtcaaa agagaagaac gaactttcag catccgggta tgcctgatgc aaggaatcga
     8281 ggaacgtatt tgtatggccg tcaccagcaa agccatgtac atggagagca gccaaatcag
     8341 tgccagcctg gtcgactggc tcgctgtgcc acccttgaag ctcaatgcca tcggtggcac
     8401 gaaagcgctc aaagtttact ttcatggagg tattatagca gtaagaacat gagaagccga
     8461 atattacgat atgtgaaatc cttatataaa ctataacgat gggggtaaag atctattctt
     8521 gctctcggac atcggcaagt gcaatatcgc caagtatgtt gacgctgcct atttcaagca
     8581 cgcgcat
//
LOCUS       DATIIX010000020         3051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_88006, whole genome shotgun sequence.
ACCESSION   DATIIX010000020 DATIIX010000000
VERSION     DATIIX010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88006"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VLF64_00590"
     CDS             complement(<1..1073)
                     /locus_tag="VLF64_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HSW90475.1"
                     /translation="MSEMIRVKGAREHNLKNIDIEIPRDKLVVVTGLSGSGKSSLVFD
                     TIYAEGQRRYVESLSSYARQFLGIMDKPDVDSIDGLSPAISIDQKSTSRNPRSTVATV
                     TEIYDYMRLLYARIGVPHCPVCGKPVHRRTAQAIIDEIMRLPEGTKVMVLAPVVNQKK
                     GEFAHVPEQYMRTGFARARVDGVIYALDEFPELQKSYKHDIEIVVDRLIVRPDAATRV
                     TQSVEQSLEIAGGVVQLLDADSNEIHTYSQRYACVDHPGEDIPELEPRLFSFNAPQGA
                     CPVCTGLGSRLEVDPDLVFNPNLTIAEGAIRPYNRVTSDAWYMKRLSAVADTHGFSLQ
                     VPVKDLKPEDLQKVLYGTGNEKYR"
     gene            1145..1492
                     /locus_tag="VLF64_00595"
     CDS             1145..1492
                     /locus_tag="VLF64_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90476.1"
                     /translation="MLKNNVADTSYIVYDGTKRRLVLMKKDFVPPLISFVAVLAITQG
                     KFTSSLINLLVAGVVPGTLIVLPSWAMIAVYCLAIATIITLYLEGTLGKARVDRSAKQ
                     HRRQLPRRRFTSI"
     gene            complement(1489..2907)
                     /gene="sbcB"
                     /locus_tag="VLF64_00600"
     CDS             complement(1489..2907)
                     /gene="sbcB"
                     /locus_tag="VLF64_00600"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HSW90477.1"
                     /translation="MSNTFFFYDLETSGLDARSDRIMQFAGRRTDMDFNPIGEPYNIL
                     IALNDDTIPSPEALLVTGISPQKTIDEGYTEAQFVKILNEEVFTPDTIAVGFNNVRFD
                     DEFVRHLFWRNFYDPYEWTYKDGRSRWDLLDVVRMTRALRPEGIEWPVDADGKPTNRL
                     ELITKANGIAHQNAHDALSDVDALIDVTKLIASAQPQLLKYLLKIRGKKEVQQIVNLD
                     DKKPFIYTSGRYDSAWNKTTVAFPIAPAPNGNTLVFDLRYDPQAFVSMTENDLAKIVF
                     ATWEERKKEGFVALPAKILQYNRCPAVAPLGVLEQGDGWQKIGLDNEVVANHMKVLLA
                     HPEFAEKLRGIFERKREFAPSTDPETQLYDGFLNDRDRLRVETVRNATERELADFHPE
                     FVDERLAPLLLHYKARNYPKTLSSSEMGTWEQWRSERLHKQLPKVLKSLQRLSTDANE
                     NHQYILQEVQLWIESVLPAEDS"
BASE COUNT          755 a          747 c          687 g          862 t
ORIGIN      
        1 cgatattttt cattgcccgt accatagagc actttctgca aatcttcagg ctttaagtct
       61 ttcaccggca cctgcaagct aaatccatgc gtatctgcaa cggcactcaa acgcttcata
      121 taccacgcgt cactcgttac gcggttatat gggcgaattg cgccttctgc aatagtaagg
      181 ttgggattaa atacgagatc aggatcgact tcaagacgac tcccaaggcc agtacatact
      241 gggcaggcgc cttgtggtgc attaaagcta aagagacgtg gttcgagctc tggtatgtcc
      301 tcacctggat ggtcgacaca cgcgtagcgc tgactgtatg tgtgaatttc attgctatcg
      361 gcatcaagta actgcacgac accgccagcg atctcaagtg actgttcgac agattgagtc
      421 acacgagtgg ctgcatcggg gcgaacgatt agacgatcga cgacaatttc gatatcgtgt
      481 ttgtagcttt tttggagctc tgggaattca tcaagtgcgt aaattacacc gtcgacacgg
      541 gcgcgcgcga atccagtacg catgtattgc tctgggacgt gtgcaaattc accttttttc
      601 tgattcacga ccggcgcaag taccattact ttcgtgcctt cgggcaagcg cataatttca
      661 tcaataatcg cctgtgcggt gcggcgatgt actggcttac cgcaaactgg acagtgtggc
      721 acaccgattc gcgcatagag taagcgcata tagtcgtaaa tttccgtcac cgtcgcaacg
      781 gtactgcgtg ggttgcggct tgttgatttt tgatcaatag agattgctgg actcagacca
      841 tcgatactat cgacatctgg cttatccata atccctaaga attgccgcgc atagctcgat
      901 aagctctcta cgtagcgtcg ctggccttca gcgtaaatag tatcaaaaac aagcgaagac
      961 tttccactac cagatagccc tgttactaca actaatttgt cacgaggaat ctcaatatca
     1021 atattcttga ggttgtgctc tcgcgcgcct tttactcgaa tcatttccga catcgagcta
     1081 attatacact aaaatcttaa ttttttccat atgcttacgc ttatttttat atgtcatatc
     1141 gcatatgctt aagaataatg ttgccgatac gtcatatata gtttatgatg gtactaagcg
     1201 aaggttggta ttgatgaaaa aagattttgt tcccccacta attagtttcg tggcagtgct
     1261 tgcgattacc caaggcaagt tcacgtcgtc gctgattaat ctgctcgtcg caggagttgt
     1321 tcctggtacc ttgatcgtct taccgtcatg ggctatgata gccgtttatt gccttgccat
     1381 tgcaaccatc attaccctct atctcgaagg tacgctcggt aaagctcgcg ttgatcgcag
     1441 tgcaaagcag catagacgcc agttgccgcg ccgccgcttt accagtatct agctatcttc
     1501 tgccggtaat accgattcga tccaaagttg aacttcttgc aatatgtatt gatggttctc
     1561 gttggcatca gtactgagac gctgcaatga tttcaatact tttggcagtt gcttatgcaa
     1621 gcgttcactt ctccattgtt cccaggtgcc catctcgcta ctagagagtg ttttgggata
     1681 gttgcgagct ttatagtgta acagtagtgg cgcaagtcgc tcgtcgacaa attcagggtg
     1741 gaaatctgca agctcacgtt cggtagcatt acgtactgtc tcgactcgta agcggtcacg
     1801 atcattcaag aatccgtcat ataattgtgt ctccggatca gtacttggcg caaattcgcg
     1861 cttacgctca aagatgccgc gtagcttctc ggcaaattca ggatgtgcaa gtaatacttt
     1921 catatggttc gcaaccacct cattatcaag tcctatcttt tgccaaccat caccttgctc
     1981 aagaacacca agtggcgcca cagctggaca acgattatac tgtagtattt ttgccggcag
     2041 cgcaacaaaa ccctcttttt tgcgctcttc ccacgttgca aatacaattt ttgcaagatc
     2101 gttctcggtc atagatacga aggcttgagg atcgtagcgt aaatcaaata ctaatgtatt
     2161 gccgtttggc gctggtgcaa tcgggaatgc cactgtcgtt ttgttccatg cactatcgta
     2221 acggccgctt gtatatatga agggcttttt atcatcaagg ttaacgattt gctgaacctc
     2281 ctttttacct cgtatcttca gtagatactt tagtaattgt ggctgagcac tagcaatcag
     2341 tttcgttaca tcaattaatg catctacgtc actgagtgcg tcatgcgcat tttggtgtgc
     2401 gataccgttc gctttcgtaa tgagctccaa cctatttgtt ggcttgccgt cagcatccac
     2461 tggccattcg ataccctctg ggcggagggc ccgggtcatg cgtacgacat cgagcaaatc
     2521 ccagcgcgag cgaccatctt tatacgtcca ctcgtacgga tcataaaaat tgcgccagaa
     2581 caaatggcgc acgaattcat catcaaaccg tacgttatta aacccgaccg caattgtatc
     2641 gggtgtgaat acctcttcgt ttaaaatctt tacaaactgt gcttcggtat atccttcatc
     2701 aatagttttc tggggtgaaa tgcccgttac taagagagcc tcggggctcg gaattgtgtc
     2761 gtcgttcaat gcaattaaaa tattgtacgg ttcaccaata gggttaaaat ccatatcggt
     2821 ccggcgccct gcaaactgca taattcggtc gcttcgcgca tcgagaccac ttgtctctag
     2881 gtcgtaaaag aaaaaagtat tcgacatata cctatagcat aacaaaaaac acgccagatg
     2941 gcgcgtatca acaaagtggt attcagctgg atagaagata ccatgtccgc cgccggccgg
     3001 agccggcccc ccgaaggggg ggtcgactcg gccggcggcg tgacaccgcg t
//
LOCUS       DATIIX010000021         3915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_449045, whole genome shotgun sequence.
ACCESSION   DATIIX010000021 DATIIX010000000
VERSION     DATIIX010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449045"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VLF64_00605"
     CDS             <1..232
                     /locus_tag="VLF64_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90478.1"
                     /translation="YGGSASLMQLEKVRLIRNIHPTVEVGWDGGANIENAFSLSQGGV
                     DVVNCGNVIHSAPDPAAVYKQLVDEINKHSVI"
     gene            266..1135
                     /locus_tag="VLF64_00610"
     CDS             266..1135
                     /locus_tag="VLF64_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HSW90479.1"
                     /translation="MSSGQLTIKQLEQKAYEVRKDIITMLEHAGSGHSAGPLDLAEIV
                     TTLYFDIMNIKPEEPEWGGRDYFFLSNGHCVPVQYAVMAERGYFPKEELMTLRRLGSR
                     LQGHPERTKLPGLENTSGPLGSGLSQASGVAYTLQYLNHETHRFVYAITGDGELDEGN
                     IWEAAMFAGKYKLSQLIVFVDRNNIQIDGPTEDIMPLEDLVGKWQSFGWHVQEIDGHN
                     VESIQDAVGMAKAITNRPSVIIAHTIPGKGVEFMEYNYHWHGAPPNSDQARQALNELR
                     TLGGKITHEGAGA"
     gene            1132..2130
                     /locus_tag="VLF64_00615"
     CDS             1132..2130
                     /locus_tag="VLF64_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HSW90480.1"
                     /translation="MSDFALNPHYAADDVKREPIRAGFGRGLKKAGEQDERIIALCAD
                     LTDSTQMGKFAAAFPERFVEIGIAEQNLVTVASGMARAGKIPFTSSYAAFSPGRNWEQ
                     IRTTIALNDQPVKIVGSHAGISVGPDGATHQMLEDIALMRVLPNMTVIAPGDSLEAEK
                     ATLALAGNGKPSYIRLAREKTPVFSTEGSPFEIGKAYILRHGDDVSLLGTGTMTYQLL
                     LLARELENEGISAEVVHVPTIKPLDETTILESVRKTGRVVTAEEAQIAGGFGGAVAEL
                     LSEKLPVPVKRLGMQDHFGESGTPEELLDHFNLSCKTMLPGVAAWIRTVPQYRQGY"
     gene            complement(2217..2606)
                     /locus_tag="VLF64_00620"
     CDS             complement(2217..2606)
                     /locus_tag="VLF64_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90481.1"
                     /translation="MACYTPEEAVVITVTLVFLVALFTYGVVVNAMYCGGSICFSRIT
                     IRELVVEIAVDAIEVLAFFYLASESKSSLVLAAAWVTLLFDIVGVIYTATNYGKQASP
                     RLWLVWLAIASDAAMIVVCVYGYTTVI"
     gene            2785..3642
                     /locus_tag="VLF64_00625"
     CDS             2785..3642
                     /locus_tag="VLF64_00625"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HSW90482.1"
                     /translation="MQRSRQQGFAVGAFNIDNQETLIAIARAAQKLNSPVLVEVSHGE
                     VEAIGLENIRDMVDNYRDEYGIEIYINLDHSPTVEACKRAIDAGFEFIHIDISQANHD
                     ATTEEIIEKTKEVVEYAKFTGALVESEPHYFAGGSNVHHEGIDYEEIKKTFSTPESAK
                     SFVDATGIDTFAAAIGNLHGKYDVPKQLDLELLAKIRANIECQISLHGGSGTPLHYFE
                     DASKQGVSKININTDMRVVFRDTLVKVLNDNPDEYAVVKLMPTVYEAVQGVVEEKISA
                     FGSAGKAVR"
     gene            3642..>3915
                     /locus_tag="VLF64_00630"
     CDS             3642..>3915
                     /locus_tag="VLF64_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSW90483.1"
                     /translation="MATPRIIAIGKGTQDVFLKSDEFDPYKEGKHMYTHLPLGLKMEV
                     EDVTFSTGGNATNVAVTFARQGLESAYVWGLGEDPASQSILDNLDKE"
BASE COUNT         1096 a          907 c         1022 g          890 t
ORIGIN      
        1 ctacggcggg agtgcaagtc taatgcagct tgaaaaggta cgacttattc gaaatatcca
       61 tccaacagtt gaggtcggct gggacggcgg cgcaaatatc gaaaatgcgt ttagtctatc
      121 gcaaggtggc gttgacgttg tgaactgcgg gaatgttatt cattccgctc cagacccagc
      181 agccgtgtat aaacaacttg ttgacgagat taacaagcat agtgttattt aatgcttacg
      241 cttaaagtgg tatactagag gcattatgag tagtgggcaa ctgacaatta aacaacttga
      301 acaaaaagcg tacgaggtac gcaaagatat tatcacaatg ctagaacatg caggcagcgg
      361 gcatagcgct gggccacttg atcttgccga aattgtgacg acactatatt tcgatattat
      421 gaatatcaag cccgaagagc cagagtgggg cgggcgcgat tacttctttt tgagtaacgg
      481 gcactgtgta ccggtgcaat acgccgtaat ggctgagcgt gggtattttc ctaaagaaga
      541 attgatgacg ctccgccgtc ttggctcacg tttacagggg caccctgagc gtacaaagct
      601 acccggtctc gagaacacct caggtccact cgggagtggt cttagccagg caagtggcgt
      661 tgcatacaca ctccaatacc ttaatcacga aacgcaccgg ttcgtctacg ctattacggg
      721 tgatggagaa ctcgatgagg gtaatatttg ggaagcagca atgtttgctg gcaaatacaa
      781 attaagtcaa cttatagttt ttgtcgatcg caataatatc caaattgacg gtcctactga
      841 agacataatg ccactagagg atcttgttgg caaatggcag agcttcggct ggcatgtgca
      901 agaaattgat ggccacaacg ttgagagcat tcaggacgcc gtgggcatgg ccaaggctat
      961 cacaaaccgc cccagcgtga ttattgcgca cacgattccc ggtaagggcg ttgaatttat
     1021 ggaatacaac tatcattggc acggcgcccc gccaaacagc gatcaagcga ggcaggcgct
     1081 aaatgagctt cgcacgcttg gcggtaaaat tactcacgaa ggagcgggcg catgagcgac
     1141 tttgcattaa atcctcacta tgcagctgac gatgtaaaac gcgaaccaat ccgcgcgggg
     1201 tttggccgcg gtcttaagaa ggccggcgag caagatgagc ggattatagc gctgtgcgcc
     1261 gaccttacgg atagtacgca gatgggcaag ttcgcggcag cattccctga acgctttgtt
     1321 gaaataggta ttgccgagca aaatctcgta acggtagcga gcggtatggc gcgtgccggt
     1381 aaaattccat tcaccagcag ctatgccgcg ttcagccctg gccgtaactg ggagcaaatc
     1441 cgcacaacaa ttgcactaaa tgatcagcca gtaaagattg tcggcagcca tgctggtatt
     1501 agcgtcggcc cagacggtgc cacgcatcag atgctcgaag atattgcatt aatgcgcgta
     1561 ctaccaaata tgacagttat agcgcctggt gacagtctcg aggctgaaaa agcaaccctt
     1621 gcacttgcgg gtaatggtaa accaagctat attcgcttag cccgggaaaa aacgccagta
     1681 tttagcactg aaggctcacc atttgaaatc ggcaaagcat atatactacg ccacggtgat
     1741 gatgtgagtc tactcggcac cggtacgatg acctaccaat tattacttct tgcgcgtgaa
     1801 ctcgaaaatg agggcatttc tgccgaagta gtacatgttc cgacgattaa accgttggat
     1861 gaaacgacga tcttagagag tgtccgtaag acaggccggg tagttaccgc tgaagaagcc
     1921 cagatcgcgg gtggattcgg tggcgcggtc gcagagcttc tctccgaaaa actaccagta
     1981 ccagtaaaac ggcttggcat gcaagatcat tttggggaat cgggtacgcc cgaagagtta
     2041 cttgatcact ttaaccttag ctgcaaaacg atgcttccgg gcgttgcggc atggattcgt
     2101 acggtaccgc aatatcgcca gggctattag ttagggcatg aaaatggccc accgagcggg
     2161 ctcaagctga atctgctcat attgaaccga ttcggcgaga ccattttctg gtgggcctag
     2221 atgacggtcg tatagccata gacgcacacg acaatcatgg cggcgtcgga ggcaatagct
     2281 agccatacga gccaaagacg cgggctggct tgtttgccgt agttggtcgc cgtgtatatg
     2341 acgccgacga tgtcaaacag caacgttacc caagcagcgg cgagtacgag acttgacttg
     2401 ctttccgatg ccaggtagaa gaatgcgagg acctcgatgg cgtcgaccgc tatttcaacg
     2461 accaactctc tgatggttat gcgcgagaag cagatgcttc ccccgcaata cattgcgttc
     2521 acaacgacgc cgtatgtgaa cagcgcaacg agaaagacca gtgttaccgt aatcacgaca
     2581 gcctcctcag gagtataaca agccatactc ggctaagtat gaactcatat attataactt
     2641 aatatatgag agacgtcaat gtattcccgc accatatcgc tagtggtata ctggtaacaa
     2701 accatactaa tttcatatcg tagggggcat catggggcta acaattacag aaattcgcga
     2761 taggacgttg cgcgcacgac atctatgcag cgttctcgtc agcaaggctt tgcagttggc
     2821 gcgttcaata ttgacaatca agaaacactg attgcgatag cgcgtgctgc acagaagcta
     2881 aactcaccgg tgttggtgga agtaagccat ggtgaagttg aggcgattgg tctcgaaaat
     2941 attcgcgata tggtcgataa ctatcgcgat gaatacggta ttgaaattta tatcaatctc
     3001 gaccatagtc caacagttga ggcttgtaag cgcgcgatcg acgctggctt tgagtttatt
     3061 cacattgata ttagccaggc taatcatgat gcaaccactg aagaaatcat cgagaaaacg
     3121 aaagaagtcg tggagtatgc taaatttacc ggcgcccttg tcgagagcga gccacactac
     3181 tttgcaggtg gcagcaatgt tcaccacgaa ggcatcgact acgaagaaat taaaaagacg
     3241 ttctcgaccc cagagagcgc aaaatccttt gtcgacgcga caggcatcga tacatttgca
     3301 gccgcaatcg gcaacctcca tggcaaatat gatgttccaa agcagcttga tctagagctg
     3361 cttgcaaaaa ttcgcgcaaa catcgagtgc caaatcagcc ttcacggcgg atctggcacg
     3421 ccacttcatt actttgaaga tgcatcaaag caaggtgtga gcaaaattaa cattaacacc
     3481 gacatgcgag tagtattccg cgacacgctg gtaaaagttt tgaatgataa cccggatgag
     3541 tacgcagtcg taaaattaat gccaacagta tacgaagcag tgcagggtgt ggttgaagag
     3601 aaaattagcg cctttggcag tgctggaaag gcagttcgct agtggcgacc cctcgtatca
     3661 ttgctattgg caagggcacg caagacgtgt tcttaaagag tgatgagttc gacccttata
     3721 aagaggggaa gcatatgtac acgcatcttc cacttgggct aaagatggag gttgaagacg
     3781 tgacattttc aacaggcggc aacgccacga atgtcgcagt cacttttgcg cgccaaggtc
     3841 tggagagcgc ctatgtatgg gggctcggag aagatcctgc aagccagtcc atcctagaca
     3901 accttgataa agagg
//
LOCUS       DATIIX010000022         6576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_450024, whole genome shotgun sequence.
ACCESSION   DATIIX010000022 DATIIX010000000
VERSION     DATIIX010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6576
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450024"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VLF64_00635"
     CDS             <1..244
                     /locus_tag="VLF64_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90484.1"
                     /translation="GAMISVAIDFLKPIRISKLVVVSPLATIPVVDQLHVSADEVHIL
                     DVKENFMGVDHYYEDNAIPSHEEVIAKINENILNWQ"
     gene            359..643
                     /locus_tag="VLF64_00640"
     CDS             359..643
                     /locus_tag="VLF64_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSW90485.1"
                     /translation="MRRKIVVVYAKYPDFHTHVRGLAKLIKEDPGNIQRELKRLEKVG
                     FLLGEKQGNTKIYSTNKQFPIFKELQSIVIKSQQQSNGSRPRRTTTDIQS"
     gene            643..2337
                     /locus_tag="VLF64_00645"
     CDS             643..2337
                     /locus_tag="VLF64_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HSW90486.1"
                     /translation="MTIFEQILKARGLDNEAAKLFFEPDYQMRHDPFLLPDMGAAVSR
                     LVEARERQEHITIYGDYDIDGLTATTVLLDAFSSFDFRFVDAFNPNRFREGYGMTEQA
                     VEEIAATGATLIVTVDCGSLSEGPIMRAKELGVDVIVTDHHNVAPVQPPAVAVINPKR
                     LLQDYPDRYEKFRLVEQVDGESLYPFLDLPGVGVAFKLVQALQTQLDGLEIGQEKWLL
                     DLVALGTVCDVVTLVDENRLHVYWGLKVLAKTRRPGLKALMGVAGAEPSHLTARTLGF
                     GLGPRMNAAGRLETAKYAQDMLLATDGMIALELAQKLDEFNVARRKAQDIIYKAALEQ
                     AEQYQEDSVLVLSHAEWSHGIIGIVAAKILERYKKPTYVFQELGDESKGSARSYGDFS
                     VADAIRASDDIITKGGGHKLAAGVTLPTQNIDAFRQRVNEYFVSLNLPPQISLLLPSA
                     DVTADDFSEATEELVTELSRLEPYGSGNPEPIICFENVTVMDVRRMGSESQHVKLSLQ
                     DTNDKKLDVVAFSAPEEWFAEVGRQVSVWVELSLNEWRGVRSVQGRLLHLVYLPEL"
     gene            2390..2605
                     /locus_tag="VLF64_00650"
     CDS             2390..2605
                     /locus_tag="VLF64_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90487.1"
                     /translation="MVQVTRKDEREANENIIRRFNRKVLQSGVLAEAKASMRFSKPLS
                     KTERRAKAIIRRERKADKLAKMRLGIR"
     gene            2614..3072
                     /locus_tag="VLF64_00655"
     CDS             2614..3072
                     /locus_tag="VLF64_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HSW90488.1"
                     /translation="MVALKERITNEMKAALLSGDRFVGETLRNLKAAILNEEVASGRR
                     AEGLGDAEVEKVIAKEVKKRFESAKIYRDNNRPELAEPEEKEAEILRKYLPQQLDETE
                     LRTIIEQKVAELGVSGASAIGQVIGGVKALVGNTADGATIARIVKETLNK"
     gene            3091..3642
                     /locus_tag="VLF64_00660"
     CDS             3091..3642
                     /locus_tag="VLF64_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HSW90489.1"
                     /translation="MILLFGPTGAGKSMQGQMLAVRKGWKWLSTGEMLRQSDDPKVIE
                     TLKSGELVSDELTYQVFEAAIQDARSKNYGNIIIDGFPRTKEQAAWLDNYMSKMGQKI
                     DAVIVLEVPEAEIMTRLEKRKRMEDTPETIARRMTIYRQKMYPVLGIFAEAGVKIVHL
                     DGTGTAGEVHDRIFDEVERTWQD"
     gene            3630..4394
                     /gene="map"
                     /locus_tag="VLF64_00665"
     CDS             3630..4394
                     /gene="map"
                     /locus_tag="VLF64_00665"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HSW90490.1"
                     /translation="MAGLITGLKTPEQIDAMRQGGAILARIFDDIRAYVKPGITEKDV
                     DTFVAGKITEYGATATYKTSEVNFPGVICISTNDEIVHGVPTDYTFRSGDIASFDLVI
                     TYKQMKTDAAFTMVVGEEVVGAIKHLINATDRSLYAGIDAVKGPVYTGDIGAAVEQVL
                     NKAKLGIIRDLVGHGVGLKMHMPPDVPNYGRKGTGVLLNPGETIAIEPMATLGGEKIV
                     TLDDDWTIATRDGSLAAHFEHTILITQDGAEILTQR"
     gene            4445..5683
                     /gene="ftsA"
                     /locus_tag="VLF64_00670"
     CDS             4445..5683
                     /gene="ftsA"
                     /locus_tag="VLF64_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HSW90491.1"
                     /translation="MQEQPRYAVGIDVGTTMIRCVVGHVDPATGTPTIVGVGQAPNSG
                     MRKGTVVNLNGPAHAIDQALGEAERMSGYEVNSATISVNGAHILSTRADGMIAVGTAD
                     KEINEDDLRRLEDVATTGKVPANREVLEVVPFSYTLDGQGNIKDPIGMSGTRLEINAN
                     VVSAMTPYVKNLEKASEMATVAPNATVPSVVAAAKAVLTEQQIESGVAVIDFGGSTTG
                     VSVFEEGDLQYVGVVPMGGVNITNDLAIGLKTDPEIAEQVKLQHGIASGGRENKLVSI
                     KQEKEILSFNSEDIDEIIGARLEEIFELINKELKKAGRAGQLPSGVVLVGGTANLKGI
                     AESAKEHLGVAARIGKSSGFGGVADSIDKPEYATVVGLMLVDAEASRERTQKSHSGPN
                     GTSLKNAGGFIQGIFSRFKM"
     gene            5764..>6576
                     /gene="ftsZ"
                     /locus_tag="VLF64_00675"
     CDS             5764..>6576
                     /gene="ftsZ"
                     /locus_tag="VLF64_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HSW90492.1"
                     /translation="MPEVKPSEIQTFASIKVVGVGGAGGSAVNRMKDAGLVGVQFIAM
                     NTDAQALHNSKADIKIHLGHNTTNGLGAGADPSVGEAAARESIDEIRDALEGADMVFV
                     TIGAGGGTGSGAGHVVAEIARDLGILCVGVATKPFSFEGEKRRTNAEWAIAQLGRQVD
                     TLITIPNDRLLQTIDRRTPLLETFKIADDVLRQGVQGISELITEHGLINLDFADVKAI
                     MSNAGSALMGIGRASGDNRAAEAAQQAIESPLIEVSIDGAKGVLFNVTGGYDM"
BASE COUNT         1801 a         1291 c         1774 g         1710 t
ORIGIN      
        1 tggtgcaatg attagtgtag caatcgattt tctgaaacca attcgtatca gcaaacttgt
       61 agtagtatcg ccccttgcga caattcccgt ggtagatcaa ttacatgtga gtgctgatga
      121 ggtgcatatc cttgatgtaa aagaaaactt catgggagtt gatcactatt atgaggataa
      181 cgcaatcccg tcacatgaag aagttattgc taaaattaac gaaaatatcc ttaactggca
      241 gtaaatctac atacgctgag gcttgcaaag acgcctgtag gaatgtagaa taaaaagcaa
      301 caatagtaag ggaatgaact gaaaggttac agtgctagac acttttatca catcaagagt
      361 gcgacgtaaa attgttgttg tatacgccaa gtatcctgat tttcataccc atgttcgcgg
      421 cctcgctaaa ctcatcaagg aggacccagg caatattcag cgggagttga agcgacttga
      481 aaaagtgggt tttttgctgg gtgaaaagca gggtaatact aagatttact ctactaacaa
      541 gcagtttccg attttcaaag aactccagag tattgtgatt aaatcgcagc agcagtcaaa
      601 tggaagtcgt ccacgacgta cgacaaccga cattcagtcg taatgacaat ttttgaacag
      661 attctaaagg ctcgcggtct cgataacgag gcggcgaagt tattttttga gcctgattat
      721 caaatgcgcc atgatccttt tttattgcct gatatgggag cggcagtttc gcgccttgtt
      781 gaggctcgtg agcggcaaga gcatattact atttatggcg actacgatat tgatgggctg
      841 acggcaacta cagtgttgct cgatgcgttt agcagttttg attttcgttt tgtcgatgcg
      901 ttcaatccga atcgttttcg ggaaggttac ggtatgaccg aacaggccgt cgaggaaatt
      961 gcagcaacgg gtgcaacact gattgtcacg gtggactgtg gcagtttgag cgaggggccg
     1021 attatgcgtg ctaaagaatt gggagtggat gtaatcgtta cggatcatca taatgttgca
     1081 ccagttcagc cgcctgctgt tgcggtgatt aatcccaagc gattgttgca agattatcct
     1141 gatcggtatg agaaatttcg tcttgttgaa caggtagacg gtgagtcgct ctatccattt
     1201 ctggatttgc ctggtgtcgg tgttgctttt aaattagtgc aggccttgca aacccagctt
     1261 gatgggcttg aaataggaca agagaagtgg ttgcttgatc ttgtggcgct tggtacggtg
     1321 tgtgatgttg tgacgcttgt cgatgagaac cggctacatg tttattgggg ccttaaggtt
     1381 ctcgctaaaa cacgtcgtcc agggctcaaa gctcttatgg gggttgccgg cgctgagcca
     1441 tcccatttaa cggcacgtac acttggattt gggctggggc cgcgaatgaa tgcggctggc
     1501 cgactggaga cggcaaaata cgcgcaggat atgctactcg caacagatgg tatgatcgca
     1561 cttgaattag cacagaaact tgatgaattt aatgttgcac ggcgcaaagc acaagatatt
     1621 atctacaaag ctgcactcga acaggcggag caatatcagg aagattcagt gcttgtgctt
     1681 agccatgctg agtggagtca tggaattatc ggtattgttg ctgctaagat tctggagcgc
     1741 tataaaaaac cgacatatgt attccaggag ctgggtgatg agagcaaggg gagtgctcgt
     1801 agctatggcg actttagtgt tgccgacgcc attcgcgcga gtgacgatat cattacgaaa
     1861 ggcggggggc ataaattggc ggctggtgta acgttgccaa cgcaaaatat tgatgcgttt
     1921 aggcagcgtg taaatgaata ttttgtgtcg ctcaatctac cgccccagat ctcgctcctt
     1981 ttaccttctg ctgacgtaac ggcagacgat tttagcgagg cgaccgaaga actagttacc
     2041 gagctttcgc gccttgagcc gtatggcagc ggcaatcctg agccaatcat atgctttgaa
     2101 aacgtaacag tgatggatgt gcgtcgcatg ggaagtgaat cgcagcatgt aaaacttagt
     2161 ttgcaagata ctaatgataa aaaacttgat gttgtggcgt tttctgcacc cgaggaatgg
     2221 ttcgcggagg ttggccggca agtaagtgta tgggtggagc tttcccttaa tgaatggcgt
     2281 ggtgtgcgtt cggtgcaggg ccgattgtta catctcgttt atcttccaga gctgtaaact
     2341 aaggtaaata gttgcaaaac agcttttcat ctgttacaat agcatcgata tggtacaagt
     2401 tacacgtaaa gatgaacgag aggcgaacga aaacattatt cgtcgcttca accgcaaggt
     2461 tctacagagc ggtgtgctcg cagaggctaa ggcttctatg cgattcagta agccactttc
     2521 taagactgag cgacgtgcta aggcgattat ccgccgcgag cgtaaagcag ataagctagc
     2581 caaaatgcgg ctaggcattc gctaggagcc cgtgtggtag cgctgaaaga gcgtattaca
     2641 aacgaaatga aagccgccct tttgagcggc gatcgttttg ttggcgagac cttacgaaac
     2701 ctcaaagcag caatcttgaa tgaagaagtt gcaagcggta gacgcgcaga aggtcttggt
     2761 gatgctgagg tcgaaaaggt tatcgcgaaa gaagtgaaaa aacgcttcga aagcgcgaag
     2821 atttatcgtg acaacaatcg cccagagctt gctgagcctg aggaaaaaga agcagagatc
     2881 cttaggaaat acttacctca gcagttagat gaaacagagc tccgcactat tattgagcaa
     2941 aaagttgccg aacttggcgt gagtggtgct tctgctattg ggcaagttat tggtggggta
     3001 aaggcgctgg tcggcaatac tgctgatggc gcaacgattg cgcgtattgt taaagaaaca
     3061 cttaataagt agcaagaggg gacgataggt atgattcttt tgttcggtcc tactggtgca
     3121 ggtaaaagca tgcagggaca aatgctggcc gtccgcaagg gatggaaatg gctttcaacg
     3181 ggagagatgc tccgccagag tgatgaccca aaggtgattg agacgctaaa atctggagaa
     3241 cttgttagcg acgagctaac ctaccaggtg ttcgaggcgg ctattcagga tgctcgtagt
     3301 aaaaattatg gcaatattat catcgacggc ttccctcgta caaaagagca ggcggcgtgg
     3361 cttgataact acatgagcaa aatgggccag aagattgatg ccgtgattgt acttgaagtg
     3421 cccgaagctg aaattatgac acgtcttgag aagcgtaagc gtatggagga tactcctgag
     3481 actatcgctc gacgtatgac gatctatcga caaaaaatgt acccagtgct tggcattttt
     3541 gctgaagcag gcgtaaagat tgtgcacctt gatggtacgg gcactgcggg tgaagtccac
     3601 gaccgaatct ttgatgaggt tgagcgaacg tggcaggatt aattacaggg ctaaaaactc
     3661 ctgaacaaat tgacgcgatg cgccaagggg gcgcaatctt agctcgtatc tttgatgata
     3721 ttcgcgcata tgtaaagccg ggcatcaccg aaaaggatgt cgatacgttt gttgcgggta
     3781 agattactga atatggtgca actgcaactt acaaaacaag tgaagtaaat tttccaggtg
     3841 ttatttgtat tagtacaaac gatgagattg ttcacggtgt accgaccgat tacacattcc
     3901 gctcgggcga tatcgctagt tttgaccttg tgattaccta caagcaaatg aagacagatg
     3961 cagcatttac gatggttgta ggtgaagaag tagttggtgc aattaaacat cttattaatg
     4021 caactgatcg aagcttatat gctggcatcg atgcagtaaa aggtcctgtt tacactgggg
     4081 atattggtgc tgctgttgag caagtactca acaaggcgaa gcttggaatt attcgagatt
     4141 tagtcggtca tggggtaggt ttaaagatgc atatgcctcc agacgtgcca aactatgggc
     4201 gcaaaggaac tggcgtattg ctaaaccctg gtgaaacaat tgcaattgag ccgatggcaa
     4261 cacttggtgg cgaaaaaatt gttacacttg atgacgactg gacaatcgca actcgtgatg
     4321 gtagtctggc ggcacatttt gaacacacaa ttcttattac acaagacggc gcagagattt
     4381 taacgcaacg ctaaagtgtt cgtagacctg tatcattatc ttgcttatgc tataatacgg
     4441 ctatatgcag gaacaacctc gatatgcagt cggtattgat gtcggtacaa cgatgattcg
     4501 ttgtgtagtg ggccatgttg atccggcgac cggcacccct actattgtgg gggtcggaca
     4561 ggcgccaaac tcaggtatgc gcaaaggaac agttgttaac cttaatggcc cagcccatgc
     4621 aattgatcag gcattgggtg aggcagagcg catgagcggc tatgaggtga atagcgctac
     4681 gattagcgtg aatggtgctc atatcttgag tacacgtgct gatggtatga ttgctgttgg
     4741 tacagctgat aaagaaatca atgaagatga ccttcgtcgt cttgaagatg tcgcgacaac
     4801 tggtaaggta ccggcaaacc gcgaggtact tgaagttgta ccatttagtt atacgcttga
     4861 tggccaaggc aacattaaag atccaattgg catgtctggc acacgtcttg agattaatgc
     4921 gaatgttgtc tcggctatga cgccatatgt taagaatctt gagaaggcta gtgagatggc
     4981 gacagttgct ccaaatgcga cggtaccgtc tgtagttgca gccgcaaagg ctgtattgac
     5041 agaacaacaa atcgagagtg gtgtcgctgt gattgatttc gggggctcta ctaccggcgt
     5101 gtcggtcttt gaagaaggtg atttgcaata tgttggcgtt gttccaatgg gcggtgtgaa
     5161 tattactaat gaccttgcaa ttggtctaaa gacagatcct gaaattgccg aacaagttaa
     5221 actgcaacat ggtattgcat ctgggggtcg agaaaataag ctggtcagca ttaaacaaga
     5281 aaaggagatc ctttcgttta actctgaaga tatcgatgaa attatcggtg cgcgtcttga
     5341 agaaattttt gagcttatta ataaagaact caaaaaagca ggtcgtgcag gccagttgcc
     5401 aagtggagtt gtcttggtcg gcggtactgc caacttgaag ggtattgccg aaagcgccaa
     5461 agagcacctt ggcgttgcgg cgcgtattgg caaatcaagt ggttttggtg gcgttgctga
     5521 tagtattgat aaaccagagt atgctactgt tgttggctta atgcttgttg acgccgaggc
     5581 gtcacgcgaa cgaacacaaa aaagtcatag cggaccaaac ggtacttcgc ttaaaaatgc
     5641 aggcggattc attcagggca tcttctcgcg cttcaaaatg taagggcctg tcattcatga
     5701 acaacaatgg tatactagaa caagatagtt atccagagtg aataaagggg agtataggaa
     5761 tccatgccgg aagtaaaacc aagtgaaatc caaacatttg ccagcataaa agttgttggt
     5821 gttggtggtg caggcggttc agcagtcaac cgcatgaaag acgctggatt agtcggtgtt
     5881 cagtttattg caatgaacac tgatgcgcag gcgctccata attcaaaagc agacattaag
     5941 atccatcttg gccacaacac gacgaatggt cttggtgctg gtgctgatcc atctgttggt
     6001 gaagctgcag cgcgtgaatc aatcgatgag attcgagatg cacttgaggg tgctgatatg
     6061 gtttttgtta ccattggtgc cggtggtggt actggatcgg gtgcaggcca tgtcgtggct
     6121 gaaattgctc gcgatctcgg tatactttgt gtcggtgttg caacaaaacc gtttagcttt
     6181 gaaggtgaga agcgccgcac gaatgctgag tgggcgattg cacaacttgg acgccaagtt
     6241 gatacactca ttactatccc taacgatcgt ttactacaga caatcgatcg ccgtactccg
     6301 ttactagaga cctttaagat tgctgatgat gtacttcgcc aaggtgtaca agggatttct
     6361 gagcttatta ctgagcatgg acttatcaac cttgacttcg ctgatgtgaa agcaattatg
     6421 agtaacgctg gcagcgcatt aatgggtatt ggccgcgcca gtggtgataa ccgcgccgct
     6481 gaggctgcac agcaagctat cgagtctccg cttattgagg tctcaataga tggcgcgaag
     6541 ggcgtactct tcaacgtaac tggtggctac gacatg
//
LOCUS       DATIIX010000023         2585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_478106, whole genome shotgun sequence.
ACCESSION   DATIIX010000023 DATIIX010000000
VERSION     DATIIX010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478106"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..82)
                     /locus_tag="VLF64_00680"
     tRNA            complement(6..82)
                     /locus_tag="VLF64_00680"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46..48),aa:Val,seq:tac)
     gene            137..826
                     /locus_tag="VLF64_00685"
     CDS             137..826
                     /locus_tag="VLF64_00685"
                     /inference="COORDINATES: protein motif:HMM:NF025881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HSW90493.1"
                     /translation="MRYKPKHYYTWSANIAYTVGLIASDGCLQKDQRHIDLTSVDMEQ
                     LNNFRLAIGRPLPITKKFNGTGQPAFRIQFSDGGYYDFLLAAGLTPAKSKTISQLTVP
                     DQYYADFLRGLFDGDGSCYGYMDPRWRSSFMFYVAFTSASLDFIHYLRAANSRICGVT
                     PGSIRKSTRAYALVYAKNDSHKLYHFMYYAPGLLCLTRKHKKLLAFTRRDRAGIISTS
                     SASGEMVNTLP"
     gene            792..875
                     /locus_tag="VLF64_00690"
     tRNA            792..875
                     /locus_tag="VLF64_00690"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:825..827,aa:Leu,seq:gag)
     gene            complement(958..1926)
                     /locus_tag="VLF64_00695"
     CDS             complement(958..1926)
                     /locus_tag="VLF64_00695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSW90494.1"
                     /translation="MLFSQASVTQARATSEMSSAADRVWKTQRYPDMIAELLEAKAEL
                     PPTILDLGCGVDEASEYADRVPTAHVLAIDANPTVIERASREFDRPNISYQVGDPLDR
                     FTMPNLDESSIGVAVLTGLLTNIIDYYDLERLFHYVDTALHSGGYIVISDYLIQPDDK
                     EAWHARYGRDLVALERAGLSGMTSRSLYGTIITRPFGYSAAAASELSVDDMFAALQQD
                     LFERFARHWNPALLMSAINNGSNGHLQVRFARVQERVYDQEDKNGLATLNGVCLVLEK
                     VTSTDPNQRQRLSNAIGRAVMSGGDHQHFFNILTQSARRELRQSDA"
     gene            complement(1988..2248)
                     /locus_tag="VLF64_00700"
     CDS             complement(1988..2248)
                     /locus_tag="VLF64_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90495.1"
                     /translation="MTFGFIDWLGYIGGAVLLFAFWRTSIGKWTGKSLWYELDNLIAG
                     ILLTVYTFDKRAYASVIINLVWAVVAFRGVTSYAERRMRTKR"
     gene            complement(2252..>2585)
                     /locus_tag="VLF64_00705"
     CDS             complement(2252..>2585)
                     /locus_tag="VLF64_00705"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HSW90496.1"
                     /translation="AHLHLQPYLDLAQIGDRQTMFRIMDEYYKLVIDLGGTTTGEHGD
                     GRLRGAYLSKLYGPEVYDVLRKTKQIFDPHGTLNPGVKIDVTLEDIKPLLRQEYSLGH
                     LHQHVPRT"
BASE COUNT          679 a          652 c          617 g          637 t
ORIGIN      
        1 gaaaatggtg ggtgatgagg gactcgaacc cccgaccctc tcggtgtaaa cgagatgctc
       61 tagccaactg agctaatcac ccgatgcagg aagtatacca tatctgtaaa taccgacaaa
      121 tggtatacta tatactatgc ggtataagcc taagcattac tatacgtgga gcgcaaacat
      181 cgcgtacacc gtcggcctga tcgccagcga tggttgtctt cagaaagacc agagacatat
      241 cgacctcacg tcagttgata tggaacaact caacaatttc cgattggcaa ttggtaggcc
      301 actgccaatt actaagaagt ttaatggaac tggccagcct gcattccgaa ttcagtttag
      361 tgatggtggc tactatgatt ttttgcttgc agctggccta acaccagcaa aaagcaaaac
      421 aatctcgcag cttactgttc ccgaccagta ttatgctgac ttcttgcgcg ggctgttcga
      481 cggcgacggt tcttgctacg gctacatgga tccgcgctgg cgttcgagct tcatgtttta
      541 cgttgctttc acttcagcaa gtctcgactt tattcactac ctaagggctg caaatagtcg
      601 aatctgtggc gtgactccgg gaagtattag aaaatctacc cgagcatacg cacttgtata
      661 tgcgaaaaac gattcgcata agctctacca cttcatgtac tacgcccccg gccttttatg
      721 ccttacgaga aaacacaaga agttgttggc cttcacgaga agagataggg ctggtataat
      781 atcgacgtca agcgcgagtg gtgaaatggt aaacacgcta ccttgaggtg gtagtgccgc
      841 aaggtttgga ggttcaagtc ctctctcgcg caccaaaata atgtctcaac agagacgtta
      901 tttttgttgt gctactacta tatatgaaat gtagaattgg atttctggga gcaggaatta
      961 ggcgtcactt tgtcgtaact cgcgtctggc agactgcgta aggatattga agaagtgttg
     1021 atgatcacca cccgacatca cagcccgccc gatagcatta gatagccgtt gacgctgatt
     1081 tggatcggta cttgttactt tctctaacac caaacaaaca ccatttagcg tggcgagtcc
     1141 attcttgtct tcttggtcat agactcgctc ttgaacacgc gcaaaacgaa cctgcaaatg
     1201 gccattcgac ccattgttga tcgcagacat gagtaatgca ggattccagt ggcgagcaaa
     1261 tcgctcaaat agatcttgct gcaaagcagc aaacatatca tcaacgctta gttccgaagc
     1321 ggccgctgca gaatatccga acggccgcgt aataatcgtt ccatacagtg atcgactggt
     1381 cataccactc aaccccgctc tttccaaggc gaccaagtca cgcccataac gggcgtgcca
     1441 cgcctctttg tcatccggct ggattaagta atctgaaata acaatatacc cgccggaatg
     1501 caaagccgta tcgacgtaat gaaatagtcg ctcaagatca taataatcaa taatgttggt
     1561 cagtaacccg gttaataccg ctacaccgat gctggattca tctaaatttg gcatcgtaaa
     1621 gcgatctagt gggtcgccaa cctgatagct tatatttggc ctgtcaaatt cacgagacgc
     1681 acgttcgatt actgtagggt tggcatcaat agccaacacg tgagccgttg gtacccgatc
     1741 tgcatattcg ctcgcctcat cgacgccaca accaaggtca agtattgttg gtggcagttc
     1801 ggccttcgct tcaagcagct cagcaatcat atccggatag cgctgtgtct tccacactcg
     1861 gtcggcagca ctagacatct cacttgttgc ccgtgcctga gtgacactcg cctggctgaa
     1921 aagcataacg ctcactcctc tattgatctg caatatagtc aatcagacgt caaagtacaa
     1981 gtggtgatta gcgctttgta cgcatacggc gttcggcgta ggacgtgacg ccgcggaaag
     2041 cgacgaccgc ccagacgagg ttgataatga cactggcata ggcgcgcttg tcaaatgtgt
     2101 acaccgtaag taagatgccg gcgatcaggt tgtccagttc ataccagagc gattttcccg
     2161 tccatttgcc gatactggta cgccagaagg cgaacagcag aaccgcaccg ccaatgtacc
     2221 caagccagtc gatgaatccg aaggtcatga attaggtccg cggcacatgt tgatgaaggt
     2281 gaccgagcga gtattcctgg cgcagcaatg gtttgatgtc ttcgagcgta acatcgattt
     2341 tgacgccggg gttgagcgta ccatgcgggt cgaagatctg cttcgtcttg cgtagtacat
     2401 catagacctc gggtccatac aatttagata aatacgcccc tcgcagccgg ccatcgccgt
     2461 gttcaccagt ggtagtaccg cctagatcga taaccaactt gtaatattca tccatgatgc
     2521 ggaacatcgt ctggcgatcg ccaatctgag cgagatccag atatggctgc aggtgcagat
     2581 gcgcg
//
LOCUS       DATIIX010000024         5754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_62254, whole genome shotgun sequence.
ACCESSION   DATIIX010000024 DATIIX010000000
VERSION     DATIIX010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5754
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62254"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..952
                     /gene="tilS"
                     /locus_tag="VLF64_00710"
     CDS             119..952
                     /gene="tilS"
                     /locus_tag="VLF64_00710"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HSW90497.1"
                     /translation="MNYLLAVSGGVDSVVLLDLFSKTNNHLVVAHVDHGIRGEESAAD
                     ARFVKELAKKYSIPFVSIELNLAPNASEELARSERYTFLFKEAKRFNALLVTAHHEDD
                     VIETIALNIERGTGWRGLMTLNRLGIMRPLLGMTKQQIYNYAMKQRLEWVEDATNHEL
                     LYARNRMRHAIKLNSVNSERLMELRARQLQLGRDIDADATRLVARHKGDRHFLNMIPE
                     NVAIELLGTEIANITTRPTRPRLKRALIAIKTAAPGSIHQIGDGISLEFTARNYTIRV
                     V"
     gene            1005..2864
                     /gene="ftsH"
                     /locus_tag="VLF64_00715"
     CDS             1005..2864
                     /gene="ftsH"
                     /locus_tag="VLF64_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HSW90498.1"
                     /translation="MPKRPKRNVRNTLRLSLFWAVIVLISLVGWALMSPRSSLKDVPI
                     SQVITDANDGKLAKIEGQDGDLTITKKGESEPSEKSYIQGGVSSLLKDDLLSKGGKQV
                     VSDKPTDGTSTAFWNVATIVVPVLLIAGFFMFMMRQAQGQNSQAMNFGKSKAKLYGID
                     KERVVFDDIAGNDSAKQDLEEVVDFLKHPKKYESLGAKIPKGVLLVGNPGTGKTMLAR
                     AVAGEANVPFFSISGSEFVEMFVGVGASRVRDLFAKAKKNAPAIIFIDEIDAVGRKRG
                     SGMGGGHDEREQTLNQILVEMDGFEAGTNVIVLAATNRADVLDPALLRPGRFDRHTTI
                     SLPERRDREAILKVHFKNKPTDSTVNLDKLAAKTAGSSGADLANIANEAAIIAARRNA
                     KHISNNDLTEAFEKVAIGPERKTKVMNEKEKEMTAYHEAGHAVVGHVLPDSDPVHKIT
                     IIPRGGTGGVTWFLPPEDRSYTNVYEFKDILARAMGGRIAEKLLYGADGITTGAGSDL
                     RKATEIARDMVIEQGMGTKLRDQVFHEDSGGMVFDKITHERPYSDATAQEIDKEVEAL
                     IREAAQRAEAVLTKNRDILEKLKEALLKEETLEEEQASEIMIDAKLPAEAKLH"
     gene            2880..4520
                     /gene="murJ"
                     /locus_tag="VLF64_00720"
     CDS             2880..4520
                     /gene="murJ"
                     /locus_tag="VLF64_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HSW90499.1"
                     /translation="MAKRSLKSVVALANSKLPLGWAALLLSGATLASSLLGILRDRYL
                     NGLYLDNYPAGIDAYTAAFTIPDFMFFILVSGALSVSFIPVFNQRLAGGNKRSAWELS
                     SSLVNFLALLTLGASVLIMIFADPLVRYIVGPGLDEQGRELAISMMRIIAVNPFLFAV
                     ATVISSMQQAVGRFAFLALAPTIYNIGILIGARYFTGGINIFGWQVFDGGIMGVALGV
                     ALGAMMQLVISSIGLIGLGFDYRMKISWRNKGFRQVLKLLPPRSLDQGIDYVNGIVEM
                     NLASRMGDGMMRAYQQASSLSLMPVNLIGVAISNAAFPRMTERLAEGRPDLFKQELRQ
                     ILRWIIWLALPIATLAFFMRGYVVTFIKNGGDPTIAGVLGALVVAILFRTVYQIAARV
                     FYAQQDTKTPLYVSFFTITLNIVLAVCFSMLLGWGVYGLAWAQSIVAFVEVVVLFVIL
                     QRRAHGIFTRAFVSAICRMVIASASMGFIAYVALQFMQLQSNDLRFFLVFPKFALITA
                     VGFIVYVWLCKLMKVEEVDPILRKLSSVIFSKIPGRRQ"
     gene            4526..>5754
                     /gene="lepA"
                     /locus_tag="VLF64_00725"
     CDS             4526..>5754
                     /gene="lepA"
                     /locus_tag="VLF64_00725"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HSW90500.1"
                     /translation="MSQQNIRNFCIIAHIDHGKSTLADRMMELTHTVEKRDMKSQLLD
                     SMELEREKGITIKLAPVSMKYKEYELNLIDTPGHVDFSYEVSRSLEACEGAVLVVDAS
                     QGIQAQTLANVYLALAADLTIIPVLNKIDLPAADIPRVTAEVINLLGCKEEDIMKISA
                     KTGAGVTDVLDAVVRRITPPTGSSDAPTRALIFDSYYDDYRGVILYTRTVDGSIKKGD
                     TIEMLATGAKGLALDVGHLRPGMSSDPELKTGEIGYIVTNLKTTRDAKVGDTITVPRA
                     HTDEALPGYQHVQPFVYAGFFPVSNEDYNDLREAVEKLSLSDSALQFDPENSPVLGYG
                     IRIGFLGLLHMDIVRERLEREYDLDLVVTNPSTNYEVTLTDGTEIDIKSASELPDVAK
                     VSEIREPWIDGEIVVPQ"
BASE COUNT         1524 a         1273 c         1482 g         1475 t
ORIGIN      
        1 ccgatgctat ggaatggaga cctgcttggc ggtatgagca ttctgcttgg ggctatcggc
       61 ggcattgtcg gcgtatggct cggcgctaaa atcggcaacg cattaggata atggtagagt
      121 gaattatctt ttagcggtat ctggtggcgt agactcggta gtgctgctcg atttatttag
      181 taagacaaat aatcatttag tggtggcgca tgtggaccat gggatccgcg gtgaagaaag
      241 tgcagccgat gcgcggtttg ttaaagaact cgctaaaaaa tacagtatac cattcgtaag
      301 tattgaactt aatctcgcac caaacgcaag cgaggaactt gcacggtccg aacggtatac
      361 attcttattt aaagaggcta agcggtttaa cgcgctactc gtgacagcac atcatgaaga
      421 tgatgtgatt gaaactattg cattgaacat cgagcgaggc acgggctggc gcggactaat
      481 gacgcttaat cgtttgggta taatgcgtcc cttgctcggt atgactaagc agcaaatata
      541 taattatgcc atgaagcagc gtcttgaatg ggtagaggac gccacgaacc acgagcttct
      601 ctatgcgcgc aaccgtatgc gacatgctat aaaactaaat agtgttaaca gcgagcgctt
      661 aatggaactg cgcgcgcgcc aattacagct cggacgcgat attgatgccg atgccacgag
      721 gcttgttgcg cgtcataaag gcgaccgaca ctttctgaat atgattcccg agaacgttgc
      781 aattgaatta ctgggaacgg aaatcgcgaa tatcacgact cgcccgactc gccctcggct
      841 taagcgtgcc ttaatcgcga tcaaaaccgc agctccgggc agtatacatc aaattggtga
      901 tggcatttca ttagagttta cggctcgaaa ttacactata agagtggtat aatttctata
      961 aagattttct ctgcagtaag aatagcgaaa ggacacttat tagaatgccg aaacgaccta
     1021 aacgaaatgt tcgtaacaca ttacgcctga gccttttttg ggcggtaatc gtattaatta
     1081 gtcttgttgg atgggcgctt atgtcgccac gcagcagcct gaaagacgta ccaatctcac
     1141 aggtaattac tgacgcgaat gatggtaagc ttgcaaagat tgagggccaa gatggcgacc
     1201 ttacaattac aaagaaaggt gagagcgagc caagcgagaa gtcatatatt cagggcgggg
     1261 tcagttccct cctcaaggat gacttactga gcaagggtgg caaacaggtt gtttctgata
     1321 aaccgactga cggaacaagc acagcattct ggaatgtcgc aacgattgtc gttccagtac
     1381 tgttgattgc tggtttcttt atgtttatga tgcgtcaggc acagggccag aacagtcaag
     1441 cgatgaactt tggcaaaagt aaggcaaaac tgtacggcat cgacaaagag cgcgtcgtat
     1501 tcgatgacat cgccggtaat gatagcgcca aacaagatct tgaagaggtt gttgacttcc
     1561 tgaagcatcc gaagaaatat gaatcattgg gcgcaaagat tcctaaaggc gtattgcttg
     1621 taggcaatcc aggtactggt aaaacaatgc tagctcgggc ggtagcaggc gaagcaaacg
     1681 taccattctt ctctatctca ggtagtgaat ttgtagaaat gttcgttggt gttggtgcga
     1741 gtcgtgtgcg tgacctattt gctaaggcta agaagaatgc accagcaatc atctttatcg
     1801 acgagattga cgccgtaggt cgcaagcgcg gtagcggcat gggcggtggc cacgatgagc
     1861 gtgagcaaac attaaaccag attttggttg aaatggatgg ttttgaggct ggcacgaacg
     1921 taatcgttct tgcggcaacg aaccgcgcag atgtacttga cccagccctt ctccgtccag
     1981 gtcgattcga ccgtcataca acaattagcc tgccggaacg tcgagatcgc gaggcaatcc
     2041 tgaaggttca cttcaagaat aaaccgacag attcgactgt taaccttgat aagctcgcgg
     2101 ccaaaactgc tggctcaagc ggagccgatc tcgcaaacat cgcaaacgaa gcagctatca
     2161 ttgcggcccg ccgtaacgcg aagcatatct caaacaatga cttaactgaa gcgtttgaaa
     2221 aggttgcgat tggtccagag cgtaagacca aggtgatgaa cgagaaagaa aaggagatga
     2281 ctgcatacca tgaggctggt catgcagtcg ttggtcacgt gcttccagat agcgatccag
     2341 tacacaaaat caccatcatt ccacgcggcg gtactggcgg tgtcacctgg ttcttgccac
     2401 ctgaagatcg aagttacacg aatgtgtatg agttcaaaga catccttgcc cgtgcgatgg
     2461 gtggtcgcat cgccgagaag cttctatatg gtgctgatgg cattactacg ggcgccggta
     2521 gtgatctgcg taaagcaact gaaattgctc gcgacatggt tatcgagcaa ggtatgggta
     2581 caaagctccg cgaccaagtc tttcacgaag acagcggtgg tatggtgttc gataaaatta
     2641 cccatgagcg accatacagt gacgccacgg ctcaagaaat cgacaaagaa gttgaggcac
     2701 ttatccgcga ggctgctcag cgtgctgagg cggtactaac aaagaaccgc gatattctcg
     2761 agaagctaaa ggaggccctt ctgaaagaag aaacgcttga agaagagcaa gcgagtgaga
     2821 ttatgataga cgcaaagctt cctgcggagg caaagctcca ctaggagtta taggagtcta
     2881 tggccaagcg aagccttaaa tcagtcgtcg cccttgctaa tagtaagctg ccccttggtt
     2941 gggcagccct actgttatct ggtgcaacac tagcatcgag cttactcggc atccttcgtg
     3001 atcgttatct gaatggcctc tacctcgata actatcctgc aggtattgac gcctataccg
     3061 cagcattcac aattccggac ttcatgtttt tcatcttggt gtcgggtgca ttgagcgtat
     3121 cgttcattcc agtattcaac caacgcttag cgggtggcaa caaacgttct gcatgggagc
     3181 ttagctcgag tctagtgaac ttcttggcat tgctcacact tggtgcaagc gtgcttatta
     3241 tgatcttcgc tgatcccctt gtacgttaca tcgtgggacc cggtcttgac gaacaggggc
     3301 gagaactcgc aattagtatg atgcgcatca tcgctgtaaa cccatttcta tttgcagtag
     3361 cgacggtgat ctcaagcatg caacaggcgg taggccgttt tgcattttta gcgcttgcgc
     3421 caactatcta taacataggt attttaatcg gtgctcgtta cttcacgggt ggtattaaca
     3481 tttttggctg gcaggtattc gatgggggga tcatgggtgt tgcccttggt gttgcacttg
     3541 gtgcaatgat gcagttagtg atcagctcga ttggtcttat cgggcttgga tttgattatc
     3601 gaatgaaaat atcatggcgc aataaaggct ttcgccaggt gttaaagctg ctgccgccgc
     3661 gctcgctcga ccaaggtatt gattatgtga acggtattgt tgaaatgaac cttgcgtcac
     3721 gtatgggcga cggaatgatg cgtgcatatc agcaggcatc atctttaagc ttaatgccag
     3781 tgaatcttat tggggtggca attagtaatg ccgctttccc acgcatgaca gagcgacttg
     3841 cagagggcag accggatctg tttaaacaag aactgcgcca aattctccgt tggattattt
     3901 ggcttgcact accaattgca acactcgcct tcttcatgcg cgggtatgta gtgacattca
     3961 tcaagaacgg cggtgaccca acaatcgccg gggttctggg tgcactggta gtggcgatac
     4021 tctttaggac agtctaccaa attgccgcgc gcgtatttta tgctcagcaa gatacgaaaa
     4081 cgccactcta cgtatcattt tttacaatca cactcaatat tgttctcgca gtttgttttt
     4141 caatgttact tggctggggg gtatatgggc ttgcgtgggc gcagtctatt gtggcgttcg
     4201 tcgaggtagt ggttcttttt gtaattttgc aacgacgtgc acacggtatc ttcacccgcg
     4261 cattcgtatc ggcgatatgc cgcatggtca tcgcgagcgc tagcatggga ttcattgcct
     4321 atgttgcgct tcagtttatg cagttacagt cgaacgacct ccggttcttc ctagtatttc
     4381 ctaagtttgc tctcattaca gctgttgggt ttattgtata tgtttggctg tgtaaactaa
     4441 tgaaagtcga agaagttgac ccaattcttc gcaaactatc atcagtcatt tttagtaaga
     4501 ttcctggccg gagacaataa ttactatgag ccaacaaaac attcgtaact tttgcattat
     4561 tgcccatatc gaccacggca aaagcacgct cgccgatcgt atgatggagc tcacgcacac
     4621 cgtcgagaag cgggatatga aaagccaatt gcttgatagc atggagcttg agcgtgaaaa
     4681 gggaattacg attaaacttg caccagtgag catgaagtat aaagagtacg agctgaactt
     4741 aatcgatacc ccggggcatg tagacttcag ctatgaagtg agccgtagtc ttgaagcctg
     4801 tgaaggtgct gtgctggttg ttgatgccag tcagggaatt caggcgcaga cgctcgctaa
     4861 tgtttactta gcccttgcag cagaccttac aattattcca gtccttaata aaattgactt
     4921 gcctgctgca gatattccac gcgtgaccgc tgaggtaatt aacctactag gttgcaaaga
     4981 agaagacatc atgaagatca gtgcgaaaac aggcgctggc gtcacagacg tgcttgatgc
     5041 cgtggttagg cgtattacgc cacctactgg aagtagtgac gcgccgacac gtgcgctgat
     5101 ttttgatagt tactacgatg attaccgtgg tgtgattcta tatacacgca cagttgatgg
     5161 gtcaatcaaa aagggcgata cgattgagat gcttgcgaca ggtgcaaagg gcttagcact
     5221 agatgttggt catctgcgcc ccggtatgtc atcagatcca gagctcaaaa caggcgagat
     5281 tgggtatatt gttacaaacc ttaaaacaac tcgtgatgca aaagtaggtg atacgatcac
     5341 cgttccgcgt gcgcacaccg acgaagcttt gcccgggtat cagcatgtgc agccatttgt
     5401 ctatgctggg ttcttccctg ttagcaatga agattacaat gacctgcgcg aagccgtcga
     5461 gaaactcagc ctgagcgact ctgcactaca gtttgaccca gaaaactcgc cagtacttgg
     5521 ctatggtatt cgcattgggt ttctaggcct tctccacatg gatattgtac gagagcgatt
     5581 agagcgtgaa tatgatctcg acttagtcgt cacaaatcca agtacaaact acgaagtgac
     5641 attgactgat ggcactgaga ttgatattaa aagcgcctca gagttgccag atgtcgcaaa
     5701 agtttctgaa attcgcgaac catggattga cggtgaaatt gtagtgccac aaga
//
LOCUS       DATIIX010000025         2313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_479259, whole genome shotgun sequence.
ACCESSION   DATIIX010000025 DATIIX010000000
VERSION     DATIIX010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479259"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VLF64_00730"
     CDS             <1..613
                     /locus_tag="VLF64_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HSW90501.1"
                     /translation="NDTGGYKEVIFMIRGDAPYSKLKFEGGVHRVQRVPVTESQGRIH
                     TSTVTVAVLPEAEETDIEISPNDLRVDIYRSSGHGGQSVNTTDSAVRITHLPTGMIVT
                     NQDEKSQIKNREKAMSVLRSRLLQIKIDEENAKLTAERRNLIGSGDRSEKIRTYNFPQ
                     DRITDHRIGYSRSNIPAALNGDIDDVIEHLQAYERELKAQNIK"
     gene            579..1412
                     /gene="prmC"
                     /locus_tag="VLF64_00735"
     CDS             579..1412
                     /gene="prmC"
                     /locus_tag="VLF64_00735"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HSW90502.1"
                     /translation="MNENSKPKTSSSNTWLQDAADQLAEAGIATARLDAEIILAHTIR
                     KSRTWLHAHGDEILTPRLIEIADARLALRLDRTPIAYIIGHKEFYSRAFKVTPSVLIP
                     RPESESLIELLKKHIPANAKRLIDVGTGSGCLGITAKLEFSSLGVTLSDVSRHALKVA
                     ESNASTLHAEVTLIQGDLLAQAPDLFDVVIANLPYVNKEWERSLETNKEPGLALFADN
                     GGLALIEQLLYQLPKALGSLGLVLLEADPCQHQAIIALATKLGLKHLETEGYAIALAK
                     R"
     gene            complement(1409..>2313)
                     /locus_tag="VLF64_00740"
     CDS             complement(1409..>2313)
                     /locus_tag="VLF64_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSW90503.1"
                     /translation="SVNTQSIFGAAEQQAAGTGIILSKDGYILTNHHVIDSATTVQVI
                     TSDGKTYENVKVIGSDPLNDVAYLKIDGVNNLTPASMGDSSTVRVGQQVVAIGNALGQ
                     YQNTVSSGIISGKGRPVTAGSGDGSSSESLTDLLQTDAAINPGNSGGPLLNYSGQVVG
                     IDTAIASDAQGIGFAIPINATKGTTKMVLAGRGVQRAYLGVRYVEITAAVAKQYNLSA
                     KSGAYVVGDGANTAIVAGSPADKAGLKEKDIITKVNGESVGVAGSVSTLAGEYAPDDT
                     ITVTYLRDGTEHNSKVTLGTYQTN"
BASE COUNT          636 a          637 c          504 g          536 t
ORIGIN      
        1 caacgacact ggcggctaca aagaagtaat ttttatgatt cgcggcgatg ctccatacag
       61 caaactcaaa tttgaaggag gcgtgcaccg tgtgcagcgc gtaccagtca ctgaaagcca
      121 agggcgcatc catacaagca ccgtcactgt tgccgtactt ccggaggccg aagaaacaga
      181 tatcgaaatc agtccgaatg atctccgtgt cgatatttac cgctcaagcg gccacggtgg
      241 tcagagcgta aacacaactg actcagcggt gcgcatcact cacctcccta ctggcatgat
      301 tgtcacaaac caggacgaaa agtctcagat taaaaaccgt gaaaaagcca tgagtgtcct
      361 gcgctcacgt ctgttgcaaa tcaagattga tgaggaaaac gccaagctta ccgccgaacg
      421 tcgcaacctc attggtagcg gtgatcgtag cgagaaaatc cgaacctaca acttcccgca
      481 agatcgcatt accgaccacc gtattggcta cagccgcagc aacatccctg cagcccttaa
      541 tggagacatc gatgatgtca tcgaacacct ccaagcctat gaacgagaac tcaaagccca
      601 aaacatcaag tagtaataca tggctgcaag atgcagcaga ccagcttgct gaagctggca
      661 tagccactgc acgcctggac gccgaaatta tcttagcaca taccatacgc aaaagtcgta
      721 catggcttca cgcccatgga gatgaaatac ttacaccccg tcttattgag attgccgacg
      781 cgcggcttgc tcttcggctt gatcgcacac caattgcata catcattggg cataaagaat
      841 tctacagtcg cgcgttcaaa gtaaccccca gcgtactcat ccctcgccca gagagcgaat
      901 cactcatcga gcttcttaaa aaacacatac cagcaaacgc taaacggctt attgatgtcg
      961 gcacaggaag cggatgcctt ggtattactg caaagctaga attttccagc cttggcgtca
     1021 ccctctcgga cgttagtcgt cacgcgctaa aagttgccga gagtaacgcc tcgactcttc
     1081 atgcagaagt aacactcatt caaggtgatc tattagccca agcccccgac cttttcgatg
     1141 ttgtcattgc caaccttccc tatgttaata aagaatggga acgctcacta gagaccaata
     1201 aggaaccggg tcttgcacta ttcgccgata atggtggtct tgcccttatc gaacaactac
     1261 tttaccaact ccccaaagca ctaggctcgt tagggcttgt gttacttgag gccgatccct
     1321 gccaacatca agcaataatt gctcttgcaa caaagctcgg cctcaaacat cttgagaccg
     1381 aggggtatgc catcgcgctt gctaagcgct agttggtttg gtatgtacca agcgtaacct
     1441 tagaattatg ctcggtgccg tcacgaagat aggtaacggt aattgtatca tctggtgcat
     1501 attcgcccgc gagcgttgaa acactaccag caacgccaac cgattcgccg ttcacttttg
     1561 taataatatc cttttccttg agtccagcct tatcagctgg actgccggca acaatcgccg
     1621 tatttgcgcc atcacccaca acatatgcac cggactttgc tgaaaggtta tactgtttag
     1681 ctactgctgc agtaatttca acataacgca ccccaagata cgcacgctgc acacccctac
     1741 cggcaagtac catttttgtc gtaccctttg ttgcattaat aggaatggcg aagccaatgc
     1801 cttgtgcatc cgaggcaata gccgtatcga taccaaccac ctgaccgctg taattcaaga
     1861 gaggaccgcc cgagttgcct ggattaattg cggcatctgt ctgcaaaagg tctgtaagag
     1921 attccgatga gctaccgtcg ccactaccag ctgtaactgg tcgcccctta ccggaaataa
     1981 taccgctcga aaccgtattc tgatactggc cgagtgcgtt accaattgca acgacttgct
     2041 ggccgacacg taccgtcgat gaatcaccca tcgaggcggg agtaaggtta ttaacaccat
     2101 caatcttaag gtaagcgacg tcattaagcg gatcggagcc aataaccttt acgttctcgt
     2161 atgtcttacc gtcagaggta atcacctgaa ccgtagtagc tgaatcaatc acatgatgat
     2221 tcgtcagaat gtatccatct ttactcaaga taatacccgt acccgcggct tgttgttcag
     2281 ctgcgccaaa aatagactgc gtattgacgc tgg
//
LOCUS       DATIIX010000026         5414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_507347, whole genome shotgun sequence.
ACCESSION   DATIIX010000026 DATIIX010000000
VERSION     DATIIX010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507347"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..872
                     /locus_tag="VLF64_00745"
     CDS             156..872
                     /locus_tag="VLF64_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90504.1"
                     /translation="MTKRQQTGFTLVEVTLAIAFLSILLIAILTITVTAGKLYIKGST
                     NQTINQSGRDFADTVRRDFLASGISMIAGSPTGAAITLGSGNLTSGRICLGTVSYLWN
                     SAALLNAPNTDEVTIGVSNTPVKFARVVNPKQSYCDKDASGKYPTNIPSTEQVIDLFG
                     GSGRDYALYSMAMTPVAITGDKGMYQISYTLGTNESGTTQKDSTNSFVQCKPNNSLTA
                     DFNYCSVDDFDMIVRVGGTK"
     gene            881..2026
                     /locus_tag="VLF64_00750"
     CDS             881..2026
                     /locus_tag="VLF64_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90505.1"
                     /translation="MSMHHNKRNAERGATALMIVVFTVLLLMTITVSFMRLVVQDQER
                     TNNDELSRGAYDSALAGVEDGKRVLQSCIGQSDTSACSAIAAGECNTIHSAKVLSVSD
                     SGANTGEVIVQNSSGTDGGYDQAYSCVKIDRNTSDYQGSLSDDSSEVVPLQTVGSFTQ
                     VTISWFKNPGTGTVFDLDASPSPLLPIATKWAAAGKTRPPLLRVQLIQYNGQNFKLDD
                     FDQSGGSNTLYLYPSSVGLSIADFSGDARRSKTNNLLIPVRCTPGIGSQYICSATLTL
                     PLPVGLPAAQIADRRAYLRVTSLYGDTDYTIQPVGTELQDVEPAIDSTGRAADVYRRV
                     RARVQLVSPAETQLYPRATVDITHNFCKDFGVSDSQYIAGSCDYAKP"
     gene            complement(2036..2950)
                     /gene="xerA"
                     /locus_tag="VLF64_00755"
     CDS             complement(2036..2950)
                     /gene="xerA"
                     /locus_tag="VLF64_00755"
                     /inference="COORDINATES: protein motif:HMM:NF040815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HSW90506.1"
                     /translation="MSELILDFVEYLEVERGRSPKTSENYRRYLERFIEFADNIKVED
                     ITNEVVRKYRLWLNRYETETGGELTTITQAYHLIALRGFLNYMSKRSIQTLAPNMIEL
                     PKIHRKQVTFLHFDEVERILAAIPNDNTLPHLRDRAILELLFSSGLRISELINLDRDH
                     INTKRREFMVRGKGQKDRPIFISQSAADAVNAYLAARVDTLVPLFLNYSRNSIASTDG
                     GYRRLTPRSIQRMIAQYAKLAGITKHVSPHTMRHSFATDLLMNGADIRSVQSMLGHSN
                     ISTTQVYTHVTDAHLKEVYEKFHSDSDK"
     gene            3043..3549
                     /locus_tag="VLF64_00760"
     CDS             3043..3549
                     /locus_tag="VLF64_00760"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - obsolete nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HSW90507.1"
                     /translation="MASTVQKTLVVFKPDTIQRGIVGEILTRFERVGLKIIGTKMITP
                     TKEHYHQHYEEIGQMITRRGQKAFDITLEMMIQGPVIAMVLEGVEAVELVRKLVGTTE
                     PKSALPGTIRGDYSHMSFGYADDAGKGIPNLIHASGDPEEAVKEIDHWFSDSELYDYA
                     VLNEKFTR"
     gene            3643..3719
                     /locus_tag="VLF64_00765"
     tRNA            3643..3719
                     /locus_tag="VLF64_00765"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3677..3679,aa:Arg,seq:cct)
     gene            3727..4257
                     /locus_tag="VLF64_00770"
     CDS             3727..4257
                     /locus_tag="VLF64_00770"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HSW90508.1"
                     /translation="MTKTSSEVFEGQHKGEEILFVFRRHIIAMRKGFYALLIPFMLTS
                     IPPLIWQQNLTLFLLPIGGLAFGLILFAYYFVMWYFTVYIVTSERIRQITQKGLFGKD
                     VVELRLAKIQNISYNIPGLTGEIFKFGTIVVQTFVGDLVIHKVEHPSEIYNKLQDAVH
                     EATSDTQQEGSDEEAN"
     gene            4241..5284
                     /locus_tag="VLF64_00775"
     CDS             4241..5284
                     /locus_tag="VLF64_00775"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HSW90509.1"
                     /translation="MKKLIKRIQRKKEEAPTPTRITNDTVAEHRERILAGGRRFKYPV
                     QYARHRLVFNTIVVSIVFIILLIAIGWWQLYPMQNSSAFMYRLTRIIPIPVAVVNSEQ
                     VPYSDYLVQYRGSEYYLNKYGEIKLDSSDGRRQLNYIKRQSLDKAEQTTYARQLARKL
                     NIVVTNDDIDKFINEERNTANGRVSQETYDSSMQLYYGETPSDYRLRIANSMLEAKIA
                     FAVDDSATAQVKQAQTLVTSTQGDLAAVATQMAKSKGGKVTAGQSGLIDVTSKFSGLR
                     VSEVAKMAKGSISGPLKSTTDDGYYFVKIVDKTGTQVDFLYLHIPLTTFTDEFAGLGS
                     SHKIQEYIKISDK"
     gene            5311..>5414
                     /locus_tag="VLF64_00780"
     CDS             5311..>5414
                     /locus_tag="VLF64_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HSW90510.1"
                     /translation="MFTLPDLSYEYNALEPVISEEIMRLHHTKHHQAY"
BASE COUNT         1492 a         1112 c         1298 g         1512 t
ORIGIN      
        1 tggagccggt gcggtgcagt ctcaagggat atggattcaa gcaacgaagg cacagcagaa
       61 cggcacagta acagcgtatg acttttatat ccatgcctgt tgggatagcg ttggtgttca
      121 tacttcaatg acgcttggca cgattgtgag gatttatgac taaaagacag caaactggat
      181 tcacgctcgt agaggttact cttgcgattg cgttcctatc tatactatta attgcaattt
      241 tgactattac cgtaacggcg gggaagttgt acattaaagg tagtacgaat cagactatta
      301 accagtctgg ccgtgatttt gccgatactg ttagacgaga ttttcttgct tcgggaatta
      361 gtatgatcgc cggttcgcca acaggagccg caattacgtt gggtagcggt aatcttacga
      421 gcggtcgcat ctgtctcggg acggttagct acttgtggaa tagcgcagca ttacttaatg
      481 cgccaaatac cgatgaagtt acgataggtg tatcaaatac tcccgtgaag tttgcacgcg
      541 tggtgaatcc gaagcagtca tactgtgata aggatgcatc cgggaagtat ccgacgaata
      601 ttcccagtac ggagcaggta atcgatttat ttggtggttc aggtcgtgat tacgcgttgt
      661 attcaatggc catgacccca gtagcgatta ctggtgacaa gggtatgtat caaatttcat
      721 atacattagg aacaaatgag agtggtacaa cgcagaaaga ctcaacgaat agtttcgtgc
      781 agtgtaaacc aaataattca ctaacagccg actttaacta ctgctctgta gatgattttg
      841 atatgatagt gcgagttgga gggacaaaat aatggtcatt atgtcaatgc atcataacaa
      901 gcgtaatgct gaacgcggtg caaccgcact catgatcgtt gtctttaccg tattacttct
      961 gatgacgata acagtcagct ttatgcggct tgtcgtacag gatcaagagc gcacaaataa
     1021 tgacgaactt tctcgtggcg cttacgactc tgcgcttgcg ggggttgagg atgggaaacg
     1081 tgtcttgcag tcttgtattg gccagagtga tacatcggct tgctctgcga ttgctgctgg
     1141 tgaatgtaac accatacatt cagcaaaagt acttagcgta agcgactcag gtgcaaatac
     1201 cggtgaagtt atagtgcaga atagctcagg taccgatggg ggatatgatc aagcttatag
     1261 ctgcgtaaag atcgatcgca atacgtcgga ttatcagggt agtctgagcg atgattcatc
     1321 tgaggtggtt cccttacaga cggtaggttc atttacccag gtaacgatat cttggtttaa
     1381 gaaccctggc acaggtacgg tatttgactt ggatgcgagt ccctctccac tcttgcctat
     1441 agccactaaa tgggctgcag cagggaagac tcgcccaccg ttgcttcggg tgcagcttat
     1501 acagtacaac gggcagaatt tcaagcttga tgattttgac cagagtggtg gatcgaacac
     1561 gctttatctg tatccttcgt ctgtcggact ttcaattgct gacttttcag gtgacgctcg
     1621 acgctccaaa acgaataacc tactgatacc tgttaggtgc actcctggca ttggaagtca
     1681 gtacatttgt agcgcaacac ttacattgcc gcttcctgta gggcttcctg ctgctcagat
     1741 tgcggatcga agagcttact tacgtgttac gagtctgtac ggcgatacgg actatacgat
     1801 ccagccagtt ggcacagagt tgcaggatgt tgagccggct attgattcaa ccgggcgcgc
     1861 agccgatgtg taccgtcgtg ttcgtgctcg cgtgcagtta gtgagtcctg ctgagacaca
     1921 attgtatcca cgtgcaacgg ttgatattac tcataacttc tgcaaagact tcggtgtatc
     1981 tgactctcag tatattgctg gttcgtgcga ctacgcaaag ccgtagctga acaatttatt
     2041 tatccgaatc agaatggaac ttttcgtata cttctttgag atgcgcgtca gtaacatgcg
     2101 tatatacctg cgtagtgctg atgttgctgt ggcctagcat actttgaaca ctccgaatat
     2161 cagcaccatt cataagaagg tcagtagcaa agctgtgacg catggtatgc ggactgacgt
     2221 gctttgtgat gccagctagc ttggcatatt gtgcgatcat acgctgaata ctgcgaggcg
     2281 ttaatcgtcg gtacccaccg tcagttgatg cgatgctatt gcggctgtaa tttaagaata
     2341 aagggacgag tgtatcgact ctagctgcta ggtacgcatt gacagcgtcg gctgcagact
     2401 ggctaataaa gatgggtcgg tccttttgac ctttaccgcg aaccataaac tcacgtcgct
     2461 tagtattaat atgatcgcga tcaagattga tgagctcaga tatacgaaga ccgctggaaa
     2521 aaagtagttc aaggattgca cgatcgcgaa ggtggggcag ggtattatca ttgggaatgg
     2581 ctgcaagtat tcgctctact tcgtcaaaat gcaggaaagt aacttgttta cggtggatct
     2641 tagggagctc aatcatgttc ggtgcaagag tttgaatact tcttttactc atataattca
     2701 aaaatcctcg aagggcaatg agatgatatg cttgtgtgat tgttgtgagc tccccaccgg
     2761 tttcggtttc gtatcgatta agccaaagtc ggtatttgcg aacaacttca tttgttatgt
     2821 cttcgacttt tatgttgtcc gcaaactcaa taaagcgctc caggtagcgc ctgtaattct
     2881 cggaagtttt aggcgaccga ccgcgttcta cttcgagata ttcgacaaag tcgagtatta
     2941 gttcggacat atacatacat ctataatact gcttgtgaca agatctagca tctaatcgta
     3001 agagtttgat acaatatagg aaataacgaa aggaccacca caatggcatc aactgtacaa
     3061 aagacactcg tagtgtttaa gccggacaca attcagcgcg gcatcgttgg agaaatcctt
     3121 actcgttttg aacgagtagg acttaagatc atcggaacaa aaatgatcac cccaacaaag
     3181 gaacattatc accagcacta cgaggagatc ggtcagatga ttactcgacg cggtcagaag
     3241 gcttttgaca ttacgcttga aatgatgatc cagggccctg ttattgccat ggtgctcgag
     3301 ggtgttgagg ctgtcgaact tgttcgtaaa cttgttggca caactgagcc aaagagcgca
     3361 cttcctggta ctattcgtgg cgactactcg cacatgagtt ttggttatgc ggatgatgca
     3421 ggtaaaggta ttccaaatct tatccatgca agtggtgacc cggaagaagc agttaaagag
     3481 atcgatcact ggttcagcga tagcgaactg tatgattatg cggttctgaa cgaaaagttc
     3541 actcgctaag taataaagat tagggcgggt ttgctcttgc agtggcttgc tttatgagct
     3601 atctctagcg attgggggat atattctata tactgtaagc atgcctcggt agctcaattg
     3661 gatagagcag ttccgtccta aggaaaaggt tgcaggttcg attcccgccc ggggtaccaa
     3721 taaactatga caaagacatc aagcgaagta ttcgaaggtc agcataaggg tgaagaaata
     3781 ctcttcgtat ttcgtaggca tatcattgcc atgcgaaaag gattctatgc tctcttaatt
     3841 ccatttatgc ttacgtctat tccgccactt atctggcaac agaaccttac attattcttg
     3901 ttaccaatag gggggttggc ttttggctta attctctttg cttactattt tgttatgtgg
     3961 tacttcacgg tgtatattgt gacatctgag cgtattcgac aaattacgca aaaaggtctt
     4021 tttggtaagg acgttgtcga attacgtttg gctaaaattc aaaacataag ctataatata
     4081 cctggtctta ccggagaaat ctttaagttc ggcaccattg tagtacagac atttgtgggc
     4141 gatctcgtaa tccataaggt cgagcatccg agcgagattt acaataagct gcaagatgct
     4201 gtgcatgaag ctactagcga cacccagcaa gaaggatccg atgaagaagc taattaaacg
     4261 aatacagcgt aaaaaagaag aagcacctac gccaacgcgt atcactaatg atactgttgc
     4321 agagcaccgt gagaggattc ttgctggtgg tcgtcgattt aagtatccag ttcaatatgc
     4381 tcgccatcgc cttgtattca atactattgt cgtctctatc gtatttatta ttttacttat
     4441 cgctattgga tggtggcagc tctatcccat gcaaaactcc agtgcgttta tgtatcgttt
     4501 aacgcgcatt attcctatac ccgtagcagt tgtgaatagt gaacaggttc catatagtga
     4561 ttatcttgtg cagtatcgtg ggtctgagta ttatctcaat aagtatggtg agattaaact
     4621 cgatagttca gatggtaggc gtcagcttaa ctacattaag cgccaatctc tcgataaagc
     4681 tgaacagact acatatgcac gtcaacttgc gcgcaaactt aatattgttg ttaccaatga
     4741 cgatattgat aaatttatta acgaagagcg taatactgca aacggtcgtg tatctcaaga
     4801 gacatatgat tcatcaatgc agctttatta cggcgaaacc ccaagcgatt accgacttcg
     4861 tattgcaaac agtatgcttg aagcaaagat cgcatttgcg gttgatgata gcgcgactgc
     4921 ccaggtaaaa caggcgcaga cacttgtcac ttcaacacag ggagatttgg ctgctgttgc
     4981 gacccaaatg gcaaaatcta agggcggcaa ggtgactgcg ggccaatcgg gcctaattga
     5041 tgtaacgagt aagtttagcg gccttcgtgt ctctgaggtt gcaaaaatgg ccaagggttc
     5101 aatatcgggt ccgctgaaaa gcacgacaga cgacggctat tattttgtaa agattgtaga
     5161 taaaactggc actcaagtcg attttcttta tctgcacatt ccactgacaa cgtttacgga
     5221 tgagtttgca ggcttagggt catctcacaa aattcaagag tatataaaga tttccgataa
     5281 gtaaaaacaa aaatacaagg gggcacctat atgtttacac taccagatct atcatacgag
     5341 tataatgcgc ttgagccggt aattagcgaa gaaattatgc gcttacatca taccaagcac
     5401 catcaagcgt atat
//
LOCUS       DATIIX010000027         3766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_340437, whole genome shotgun sequence.
ACCESSION   DATIIX010000027 DATIIX010000000
VERSION     DATIIX010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340437"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1675)
                     /locus_tag="VLF64_00785"
     CDS             complement(128..1675)
                     /locus_tag="VLF64_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HSW90511.1"
                     /translation="MDKDELLRVAKQHYSQTQLGRLSDAVDYASAKHEGQLRKCGEPY
                     IVHPLSVASSIIEWGMDIDSVIAGVLHDTVEDTDASLEEIERLFGRDVAFLVDGVTKV
                     GQARSGMRNLESYLPSTKDNLTKLLIAVGQDVRVIIIKLADRLHNMQTLQFKSVEKQK
                     KIARETIEVFAPLADRLNMGRVRVQLEELSFRYLSPGEFEKTKSLMDSHLKKSERKLR
                     SVRNEVDAHLAAEGIPHQIDGRVKSIYSLYKKLARVPNIDDIYDLMALRIIVDDVATC
                     YLVLGELHSMYEPMVDRIKDYIAKPKPNGYQSLHTTVATKTGQAVEFQIRTHEMHDYA
                     ERGLAASFHYNEQKLTDAYTKGTIAALPTDLHWIRHLQSAAARIKEGKDFDQADLTID
                     LFGDRIFVHSPKGDIYDLPAGAYPLDYAYRVHSDLASKASGFIVNGKMEPFSYRLKHG
                     DVIEILTKKSAWPKVAWQQYMITAHARMKLRAQLRKGGLLGQLSEAAAIIHRKARERR
                     NKRAKDK"
     gene            1762..2778
                     /gene="gap"
                     /locus_tag="VLF64_00790"
     CDS             1762..2778
                     /gene="gap"
                     /locus_tag="VLF64_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HSW90512.1"
                     /translation="MSTRIAINGFGRIGRNAFKIAFDRNDVEVVAVNDLTDTKTLAHL
                     LKHDSSYGTYAHEVGHDDTGIIVDGKHITVFAEKDPAALPWRDHDIDVVIESTGFFVK
                     PELAKAHITAGAKKVVISAPAKGDGADTIVLGVNEDKIANSTEVISNASCTTNCITPV
                     MAVLETNFGIEKALMTTVHSYTASQRLQDAPAKDLREARAAAENIVPTTTGASIAAAK
                     ALPALENKFGGLSIRVPTPVVSVSDFSVLLKKDVTVEELNQAFKDAAAKPFYQGILAV
                     TEEELVSSDFKGNSHSCIIDLPLTNVVGGNLVKVVAWYDNEWGYSNRLVELVADAGRA
                     VKNI"
     gene            2872..3333
                     /locus_tag="VLF64_00795"
     CDS             2872..3333
                     /locus_tag="VLF64_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HSW90513.1"
                     /translation="MKIFLGADHGGFYLKEKIEAYLSKRGFDWEDVGDKELDPGDDFP
                     QFAQMAATKVLGEEESTDPRAVLICTGGQGMAMAANRFRGIRAAVIWDKFEAHECRND
                     NNSNVLCLPARVVDAPGDDLEIWKSIIDEWLETPFAGAARYVRRNAELDAF"
     gene            3336..>3766
                     /locus_tag="VLF64_00800"
     CDS             3336..>3766
                     /locus_tag="VLF64_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90514.1"
                     /translation="MSEIAPTILAETPDDYKATVEQYHAFAKRVHIDLTDGEFAPTFT
                     VGVNQLWWPQEWQVDIHAMVSHPSQYVDALIQMKPYSIIFHAETNEDLLPTIQKIKAA
                     GIKAGVALLKQTVPEMVHNYVENADHVLVFSGNLGHYGGSA"
BASE COUNT         1010 a          906 c          911 g          939 t
ORIGIN      
        1 gccggttcct ccaatcacac cgcccgcacc acagccacct gaaacagcgg gtgaaccaaa
       61 tcaacctcga ccaccgcagt tctaaagtga agtaacacac taaatagagt cagaattata
      121 tctggctcta tttatcttta gcacgtttgt tgcgccgctc tcgagctttg cggtgaatga
      181 ttgctgccgc ttcactaagc tggccaagta gaccgccctt gcgcaactgc gcgcgaagtt
      241 tcatacgtgc atgtgcagta atcatatatt gctgccaggc gactttaggc caggcgcttt
      301 tcttagtaag aatctcaatg acatcgccgt gcttgaggcg atagctgaac ggctccattt
      361 tcccattaac aataaaccca ctggccttag atgccaagtc tgagtgtacc cgatacgcat
      421 agtcgagcgg atacgcacct gccggcaggt cataaatgtc gccttttggt gaatgtacaa
      481 agatacgatc cccaaagaga tcaatggtaa gatcggcttg atcaaaatcc ttaccttctt
      541 ttatgcgggc cgctgcactt tgcaagtggc gaatccagtg aagatcagta ggaagtgctg
      601 caatagtgcc cttggtgtat gcatctgtta gcttttgctc attgtagtgg aagctcgcgg
      661 caagtccgcg ctcggcataa tcatgcatct cgtgggtacg aatctgaaac tccaccgcct
      721 gaccggtttt tgtagcaact gtcgtatgta gtgattggta gccgtttggc tttggtttag
      781 cgatgtaatc tttaatgcga tcaaccatcg gctcatacat actatgaagt tcaccgagca
      841 caagataaca ggttgccaca tcatcaacaa taatgcgtag agccatgagg tcgtaaatat
      901 catcaatatt gggaacgcgc gcaagctttt tgtagagact gtagatactc tttacccgcc
      961 catcaatttg atgcggaata ccttcggcgg cgagatgtgc atcaacttca ttacggacgc
     1021 tgcgcagttt gcgctcactc ttttttaggt ggctgtccat gagtgatttt gtcttctcaa
     1081 attccccagg ggaaagatag cgaaaactca gctcttcaag ctgaacacgg acacggccca
     1141 tattgaggcg gtccgcaagc ggagcgaata cctcgattgt ctcgcgagct atctttttct
     1201 gcttttcgac actcttaaac tggagcgtct gcatattatg aagacgatca gcaagcttaa
     1261 taatgatcac ccgcacatcc tgtcccacag caatcagaag cttggtgagg ttatctttag
     1321 ttgatggcaa gtaactttca aggttgcgca tgccactacg tgcctggcct accttggtga
     1381 cgccatcaac gagaaacgca acatcacgtc cgaataacct ctcaatttcc tcgagtgagg
     1441 catcggtgtc ctcgacggta tcatgtaaca cgcccgcaat cacagaatcg atatccatgc
     1501 cccattcaat aatggatgaa gctacagaga gagggtggac aatatagggc tcgccacact
     1561 ttctgagctg gccttcgtgc tttgctgatg catagtctac tgcgtccgaa agacgcccga
     1621 gttgagtctg gctatagtgt tgtttagcaa cccggagcag ctcgtcttta tccatctgtc
     1681 ctcagtatag cattcttata taccttgttt gcctagtgat gtatactaga gacaacaagc
     1741 taatgctaaa aggggagaga catgagtaca cgaatagcaa ttaatgggtt cggccgtatt
     1801 gggcgtaacg catttaagat cgctttcgac cgcaacgatg tagaggtggt ggccgtcaat
     1861 gatcttacag acacaaagac attggcgcac ctactgaagc acgacagcag ctacggcacg
     1921 tacgcacacg aggtcggaca tgatgacact ggcattattg ttgatggcaa gcacatcact
     1981 gtgtttgcag aaaaagatcc cgcagcattg ccgtggcgtg accatgacat cgatgttgta
     2041 atcgaaagta ccggattctt tgtaaagcct gagctcgcta aagcacatat caccgctggc
     2101 gctaagaaag ttgtgatttc tgctcctgcg aaaggtgatg gcgctgatac aatcgttctc
     2161 ggcgttaacg aagataaaat cgcaaactca actgaggtta tctcaaacgc aagttgtaca
     2221 actaactgca tcactccagt aatggcagtt cttgaaacaa acttcggtat cgaaaaagcc
     2281 ctaatgacaa ccgttcacag ctacacggcg agtcagcgtt tgcaggatgc accggcgaaa
     2341 gaccttcgtg aagcccgcgc agcggctgag aacattgtcc caaccacaac tggcgcgtca
     2401 atcgcagccg cgaaggcact tccagcactt gaaaacaagt ttggcggtct tagtattcgt
     2461 gtaccaaccc ctgtagtgag tgtgagcgac ttctcagtac tccttaaaaa agatgtgaca
     2521 gttgaagaac taaaccaggc atttaaagat gctgcagcga agccattcta ccaaggtatc
     2581 cttgcggtaa ctgaagaaga gctcgtatca agtgacttta aaggcaacag ccacagttgt
     2641 attatcgatc ttccgcttac aaacgtagta ggtggcaacc ttgtgaaggt agtcgcatgg
     2701 tatgacaatg agtggggtta ctcaaaccgt cttgttgaac ttgtcgctga tgcaggtcga
     2761 gcagtaaaga acatctagtc tataggtgca atagcaagaa tccgccaggt aacactggcg
     2821 gattcttgcg gtaactttac agccttttgc ttaccgctta tacttactaa tatgaagatt
     2881 tttcttggcg ccgatcatgg cggattttac ctcaaagaaa agatagaagc gtatctctca
     2941 aagcgcggct ttgattggga agatgttggc gacaaagaac tcgacccagg ggacgatttc
     3001 ccgcagtttg ctcaaatggc agcaacaaaa gtattaggcg aagaagaaag caccgacccg
     3061 cgcgcggttc ttatctgtac tggtggccaa ggtatggcca tggctgcaaa tcgatttcgc
     3121 ggcattcgtg cagccgtaat ctgggataag tttgaggcgc acgaatgtcg caatgataac
     3181 aactcaaatg tgctctgctt gccagctcgt gttgttgatg caccaggtga tgatcttgag
     3241 atttggaaat caattattga tgagtggctg gagacaccat ttgctggggc ggcgcgctat
     3301 gttcggcgca acgcggaact ggatgccttt taacgatgag tgaaattgcc ccaaccatct
     3361 tggcggagac ccccgacgac tataaagcga cagtcgagca ataccatgcg ttcgcaaaac
     3421 gtgtacatat cgatcttaca gacggcgaat ttgcccccac ctttacagtg ggcgtcaatc
     3481 aactttggtg gcctcaagaa tggcaagtgg acatccatgc gatggtgtcg cacccaagcc
     3541 aatatgtcga tgcgctcatt cagatgaagc catatagtat catttttcat gccgaaacga
     3601 atgaagattt attgccaaca atccaaaaaa tcaaagcggc aggcataaag gctggagtcg
     3661 cccttttaaa gcagacggtt cctgaaatgg tgcataacta cgtagaaaac gccgatcatg
     3721 tactcgtatt ctcgggcaat ctgggtcact acggcgggag tgcaag
//
LOCUS       DATIIX010000028         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_535091, whole genome shotgun sequence.
ACCESSION   DATIIX010000028 DATIIX010000000
VERSION     DATIIX010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535091"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VLF64_00805"
     CDS             <1..344
                     /locus_tag="VLF64_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90515.1"
                     /translation="KLSRWLIRFTPYNYNIWGDAYANIVLELDRRRQNLLGDYKNLKC
                     PVTMVLAEESAGSTGERAMRNNKIWHDGAARLVKQNPSISVEWLQGSHQLPFKHFAEL
                     AEVIDTFASNL"
     gene            complement(551..1015)
                     /locus_tag="VLF64_00810"
     CDS             complement(551..1015)
                     /locus_tag="VLF64_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90516.1"
                     /translation="MSTPILDVTIRDLNLEVVHDRLAYRATEGREALAPPVPLKALEA
                     AIKDYRRWLYLRLEFPEARVVPSDHIEEVWRQHAEFTEKYRGDCAVIFGRGMFLDRRP
                     YFTRNVLSMEEEDLSTTRGLWADVFGEVPASYENMVPPLTGWGGAVVSLMAE"
     gene            complement(1322..1855)
                     /locus_tag="VLF64_00815"
     CDS             complement(1322..1855)
                     /locus_tag="VLF64_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90517.1"
                     /translation="MSLRNTLSRIRTNWIVLFAAGIVMLLMVVAYMLFTQQLHAPFRN
                     SEMLMWKELASLHMADSAPDNGHFHDGAGLDNLPEITGTYIIPRTTIGEERSKLGAIL
                     EAHGYRDIQQGYPQNIQLTPNTSDFMGNKDRFAIMIHLGSETFGNGIAGTYRPTQDSD
                     AITSFTFDIYDHGDSTH"
     gene            1990..>2289
                     /locus_tag="VLF64_00820"
     CDS             1990..>2289
                     /locus_tag="VLF64_00820"
                     /inference="COORDINATES: protein motif:HMM:NF037099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90518.1"
                     /translation="MKITTAKLRKFNMIMAALHAAQGILVLALSRSFDLPISGNYLTF
                     NQRVQQLEPTNPILFHLSLPMLVAMFFFISAFAHLIIATTYYRRYERDLKRGINKA"
BASE COUNT          599 a          581 c          533 g          576 t
ORIGIN      
        1 tgaagctaag tcggtggtta attcgtttta caccgtacaa ttacaacatc tggggagatg
       61 cgtacgccaa tattgtgctc gaattggaca ggagacgcca aaaccttttg ggtgattaca
      121 aaaacctgaa gtgtcccgta acgatggtgc ttgcggaaga aagcgcgggt tcaaccggcg
      181 agcgcgccat gcgcaataac aaaatctggc acgatggagc cgctcgtctc gtaaagcaaa
      241 acccatcaat ctcagttgaa tggttacaag gcagccatca acttccattt aaacactttg
      301 cagagcttgc agaagtaatt gatacatttg cttcgaatct gtaattacta tataaagaat
      361 ctaagttttt catagtacac aaaggttatc tcaggccatt gaagcgacag agactccgac
      421 aatctttcgt atccaacgcc agagtagaca aaaagcgcag aagcgcttgc ttgattcagg
      481 tggtacaacc ggcccgtgtg cacgctaatg gtgcaacacg ggccgggtgg gcttgttggc
      541 cctcaggatg tcactcagcc atgagcgaca ctaccgcacc gccccacccc gtgaggggcg
      601 ggaccatgtt ctcgtagctg gcgggtacct cgccgaagac gtcagcccac agcccgcgcg
      661 tagtggacag atcctcctcc tccatggaga ggacgttccg ggtgaagtac gggcgccggt
      721 cgaggaacat accgcgaccg aagatgaccg cacagtcacc tcggtacttc tcggtgaact
      781 cggcgtgttg gcgccagacc tcctcgatgt gatcagaggg gacgacacga gcctctggga
      841 actccagacg caggtacagc cagcgccggt agtccttgat cgccgcctcc agcgccttca
      901 gcgggactgg cggggcgagc gcctcccggc cttcggttgc ccggtaggcc aagcggtcgt
      961 gcacgacctc gaggttgagg tcgcggattg tcacgtccaa gatgggcgtt gacattgtct
     1021 tgcctccaat ggaatcctga gaccccggta atttaccgag atcatgtagc tatttatgtc
     1081 acatttacag tagactttaa agtgtagaat ttacagcgag ccagcaatca tctacttttt
     1141 atatattagt tctacaatac caagcagcat catagtagac ccacatcctt cggagaaagc
     1201 aagctacact accataatgt actgcgctgt gtcgctgatc atgccaaggg ctgctcaact
     1261 cttatcgacc ccgcactaga gctcttcctt tttatgtcgc agggtcggtc tctcggcaag
     1321 actaatgggt cgaatcacca tggtcatata tatcaaaagt aaaactagta atcgcatcgc
     1381 tatcttgcgt aggacgatag gtgcccgcga tgccattgcc aaaagtttca gagcctaggt
     1441 gaatcataat agcaaaccga tctttattac ccataaaatc actcgtattt ggggtaagtt
     1501 ggatattttg agggtagcct tgctgaatat ctcgatatcc atgcgcctca agaatggcac
     1561 ccaatttaga gcgctcttcg ccaatggtgg ttcgcggtat aatataagta ccagtgatct
     1621 ctggaaggtt atccaaacct gcaccatcgt gaaagtgtcc attatctggg gcgctatctg
     1681 ccatatgaag actagctaac tctttccaca ttagcatctc tgagttgcgg aaaggggcat
     1741 gtagttgctg tgtgaacaac atgtacgcaa caaccattag cagcattaca atccccgcgg
     1801 caaatagcac aatccagttc gttcgtatac gagataaagt gttgcgtaaa ctcatgttcg
     1861 tattataacc gatcaccact atcagtctat atctcatctt agcagggcaa acgccttatt
     1921 aattcgatca gttactatta cttttatagt caccctgctg caacatgttt atgctaaaat
     1981 aagctcatca tgaaaattac aacagctaaa ctgcgaaagt tcaatatgat catggctgca
     2041 ctacatgcgg cacaaggaat attagttttg gcacttagtc ggtcatttga cctgccgatt
     2101 tcgggcaatt atcttacctt caatcagcgt gtacagcagc ttgagcccac aaacccgata
     2161 ctgtttcatc tctcacttcc aatgcttgtc gctatgtttt tcttcatatc ggcgtttgct
     2221 catctgataa ttgcgactac ctattatcgc cggtatgagc gtgaccttaa acgcggcatt
     2281 aacaaagcc
//
LOCUS       DATIIX010000029         2171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_368904, whole genome shotgun sequence.
ACCESSION   DATIIX010000029 DATIIX010000000
VERSION     DATIIX010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368904"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="VLF64_00825"
     CDS             <1..1032
                     /locus_tag="VLF64_00825"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HSW90519.1"
                     /translation="GKINVYFNCVGGGEGNVEADRTALTKEAQFIGSTVLGMDIQYGV
                     NVNYTVMRDLVSALGGITVTIESRDPRGQMDSNFDWKCGVGDPKVSRAEELQRCPPSG
                     HYIDYPNGSVNLDAEHALYLAQARGDLAPTYGFEQSNFDREKNQQKIVKAIREKAMSA
                     GVLADFGKVSKILDALGNNMRTTFATNEVRTLVSLAKDIKDADIKSLSFIDGNPPLMT
                     GNAQPTAGMYQFSDIKAFIAKNLSSNPVAREGAKIAVFNGSETAGVAQTEADKLTSAG
                     FTVSAVDNAPEGTYADVEIYQIGSGNTATKTKLESMFNVKTKTTEPPVTVADGTNFVV
                     IFGKARSTE"
     gene            1059..1838
                     /locus_tag="VLF64_00830"
     CDS             1059..1838
                     /locus_tag="VLF64_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90520.1"
                     /translation="MEFLKSSKRRSVLSESVYVVLNIALAVAILSVVSAIESPLPAFG
                     LVLLSKWRILAVRPRYWFANVQANMVDIIVSLSFVVLLYAANRAFIVQCLLTLVYIVW
                     LLFVKPRSKRIYVAVQAGAALFLGVTALMSVSFDWMSSAVVLFMWVIGYSAARHVLGS
                     YEEAHISFYSLVWGLVIAELGWLMYHWTFAYSIPGAGDIKLSQGALIVLAISFLAERV
                     YSSYTRHGAVRSGDVVLPALLSVSVVIILLAFFNTISSAAV"
     gene            1884..>2171
                     /locus_tag="VLF64_00835"
     CDS             1884..>2171
                     /locus_tag="VLF64_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90521.1"
                     /translation="MNESHSQDSQQNKSMETPTPPQKTTSVVSRPRYSKRKLLLIVAI
                     IAGCIVSAFAGAEIRSLLMQQAGTVTSRETTQNDGNKVVTQEEEDIANVVSK"
BASE COUNT          573 a          433 c          563 g          602 t
ORIGIN      
        1 ggcaaaatta acgtgtactt taactgtgtc ggtggtggag aaggtaatgt cgaggctgat
       61 cgtacggcgc tgacaaaaga agcgcagttc attggtagca cagtactggg gatggatatt
      121 caatatggtg tgaacgttaa ctacaccgtg atgcgcgatc ttgtaagcgc actcggcggt
      181 attacggtaa cgattgaaag tcgcgaccct cgtggtcaaa tggatagtaa ctttgattgg
      241 aaatgtggcg tgggtgatcc taaggtgagc cgtgctgaag aacttcagcg ttgtcctcct
      301 agtggtcact acattgacta tcctaacggt tctgttaatc ttgacgctga acatgcactt
      361 tatcttgcgc aagctcgtgg agacctagca ccaacatatg gatttgaaca atccaacttc
      421 gatcgcgaga aaaaccagca aaagattgtg aaggctatcc gcgagaaggc gatgagtgct
      481 ggagtgttag cagactttgg aaaagtgagc aagattctcg atgcacttgg aaataatatg
      541 cgtacgacat ttgcaacgaa tgaagtaaga acgctcgtat ctctcgcgaa agatattaaa
      601 gatgctgaca ttaagtcact aagctttatc gatggcaatc ctcctttgat gacgggtaac
      661 gctcagccta ctgcaggaat gtaccagttt agtgacatca aggcctttat cgctaagaat
      721 cttagttcta atcctgtggc tcgtgaggga gcgaagatag cggtgttcaa cggctctgag
      781 actgcgggtg tagcgcagac agaggctgac aagctcacaa gcgcaggatt cacggttagt
      841 gcagtggaca atgcccctga aggtacgtat gcggatgtcg aaatttacca gattggcagc
      901 ggaaacacgg caacaaagac aaaattggaa tcaatgttta atgtgaagac aaagactact
      961 gaacctcctg tgactgtagc tgatggaaca aactttgtcg tgatcttcgg taaagctcgc
     1021 tcaactgagt aaatcaagtg ctataataga gaagaattat ggagttttta aaatcatcta
     1081 agcgacgttc ggtactaagt gaatctgtct atgtcgtatt aaacatagcc cttgctgtcg
     1141 cgatcctgtc ggtagtgagt gcaattgaat ctccgctccc agcttttggt ctcgtacttc
     1201 ttagtaaatg gcgaattctt gctgtgcggc ctcgatactg gtttgccaat gtacaggcaa
     1261 atatggtaga tatcattgtt agtctaagct ttgtggtgtt gttgtacgcg gcaaaccggg
     1321 ctttcatagt ccagtgtctt ctcactttgg tatacattgt atggctgttg tttgtaaaac
     1381 ctcgctcaaa gcgtatatac gttgctgttc aagcaggtgc agctttattc ttaggtgtta
     1441 cagcacttat gtcggtgtcg tttgattgga tgtcatctgc cgtagtcttg tttatgtggg
     1501 ttatcgggta cagcgcagca cgtcatgtgc tcggtagcta tgaagaggct catatttcgt
     1561 tctatagtct tgtatgggga cttgtaattg ccgagctcgg atggttaatg tatcactgga
     1621 ccttcgcgta cagtatcccg ggtgctggtg atattaagtt atcgcagggg gctcttatag
     1681 ttctcgcaat tagtttcttg gcagaacgtg tctatagcag ctatacgcga cacggagcgg
     1741 tccgttcagg agatgtggta ttgccggcgc tattatcggt aagcgttgtc attattttat
     1801 tggcattttt taatacgatt agcagcgctg ccgtttaagc cacacttcag tttctggcgg
     1861 tagcatacga ggagatgaac gacatgaatg aatcacactc tcaggactca cagcaaaata
     1921 agtcgatgga gacgccgaca ccaccgcaaa aaacgactag cgtcgtttcg cggcctcgtt
     1981 atagtaaacg aaagttgtta ctgatcgtag ctattatcgc gggttgtatc gtatcggcat
     2041 tcgccggtgc cgaaattcgc tctttactta tgcagcaagc gggcacagtg acgagtcgtg
     2101 aaacaactca aaatgatggc aataaggttg tgacacagga agaagaagac attgccaatg
     2161 tggtaagcaa a
//
LOCUS       DATIIX010000030         7026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_369166, whole genome shotgun sequence.
ACCESSION   DATIIX010000030 DATIIX010000000
VERSION     DATIIX010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7026
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369166"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..593)
                     /locus_tag="VLF64_00840"
     CDS             complement(18..593)
                     /locus_tag="VLF64_00840"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HSW90522.1"
                     /translation="MRYIADLLTLARLVLAFVVPGCILQHEWHLALILFGAAALSDAL
                     DGWCARCWPYTPEEEARLPWRRIDHHALDNGPDALMLALAVAALAFIIDYWWMLALLI
                     YGVGALFFVAVQLFIHRGKAKVAEVTDVVFGYWFVINIGMVAMEMAYRADVMVWTLGV
                     GLGILAVLLPLKWARVVSRPETREAAMKHAK"
     gene            complement(752..1585)
                     /locus_tag="VLF64_00845"
     CDS             complement(752..1585)
                     /locus_tag="VLF64_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90523.1"
                     /translation="MLREDEVAFLLPGILSRGENFQALGIPTDGVAEYGMFGPNCFKP
                     DWSVRGAALDVLNSLRRGKKVLLVGASLGGMLVPFVVREIRELLIPDEDVLSGLRCVI
                     IDAPSGAKTMKAVGPFASRVITSPFGHLLRPLAHVKVLPKDDYIEVPDTETMREIAGE
                     AMSGVAWRAYIKGRAKQELGGHSSKLWLEQLRWMIRVGDDGELTKVCDLEKACASLAG
                     IDVAYLQCCGAGNDVVTPDASGWWHDVVPGIKMFWVEAAHCGFVQQAPTFRGALTKIL
                     G"
     gene            complement(1714..2535)
                     /locus_tag="VLF64_00850"
     CDS             complement(1714..2535)
                     /locus_tag="VLF64_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HSW90524.1"
                     /translation="MQNSLPKLVTFDGEARSGKGTVVGLVKDYLRDVEGYSVMFIDAG
                     QVFRVLVVAITRAGVDIGSPAAVDAYLGDDANIAACVQLVKDVYHMPKSERESLLYTS
                     EVGVNSAKVGARPLSQTFKDNLLRKWLRDARAEGFDIVLLDGRALEEIGVMLEREGLC
                     KYILGLFFVCDPIVSAQRTLGQMPKPYNELEGDFKEQVDTLVAQIKERNRADSERAVQ
                     PVVMPRGAPRYIVTDVPDEIRGYKDRPMVIIDRSPEIPFDIMGVPTAKLIAHYVV"
     gene            2604..3398
                     /locus_tag="VLF64_00855"
     CDS             2604..3398
                     /locus_tag="VLF64_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HSW90525.1"
                     /translation="MKLYSWNVNGIRAVLTKGALQDFITKHDPDILCLQETKAERGQF
                     EIDLPQYEEHFFSAAKKGYAGTAIFSKIPALRYIDGFPETIIKQYNVTGDTYGDPNKE
                     GRIIAAEFEDFWVVTCYTPNSKGDLSRLSLRYDSWDHACLAYLQQLEQTKPVLYCGDM
                     NVAHEPIDLANPKANVGKHGYTNEERERFDDYLAAGFIDTYRTANPDKVGAYSWWTHW
                     ANARARNVGWRIDYWLASKAIAGKIKNPQIHPDVMGSDHCPISIEL"
     gene            3464..4255
                     /locus_tag="VLF64_00860"
     CDS             3464..4255
                     /locus_tag="VLF64_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90526.1"
                     /translation="MHDYWKQQQPTQPLFTDIEWSKPEQKASRGRLGIIGGNKLGFAG
                     VAESYSTALNTGAGEVRVLLPDCLRKSIPPSMTDVIFAPCNPSGSLSRDAMPDLTALG
                     NWASCLLLAGDAGRNSETAITYSDLIRNFSGQLVITRDAVDLVKNDAEALVNRPNTVL
                     IVSFAQLQKIFQAVYYPKMLTFSMQLLQLVEAIHKFTVTYPITIAVLHRDTLIVAHNG
                     EVVTTKWETPLAIWRGITAARAATYLMWTPGQPLQAIATSLVTKE"
     gene            4294..4380
                     /locus_tag="VLF64_00865"
     tRNA            4294..4380
                     /locus_tag="VLF64_00865"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4328..4330,aa:Leu,seq:caa)
     gene            4449..5738
                     /locus_tag="VLF64_00870"
     CDS             4449..5738
                     /locus_tag="VLF64_00870"
                     /EC_number="3.4.22.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HSW90527.1"
                     /translation="MRPDENRPATPLSVPARQRALISSSRVQDNKEAAANIVRNQIDT
                     IYQNDPSATTEAGAQPTITPGIAQLEPEAIQTTPAKSPITPGPGAINMQAANQQAEAI
                     NPYGRTHDESEHGTPTDAWRKYHSAWQSYYQQYYERYYVGQVHNVKRSLEKDVNASTN
                     DAPETISGDEAMYDLRSKLRNQINERAQKVRKSRHFIPIISALCVMLVFVFLQYNRLM
                     FAAIDAYISPGSLDPSTLIIDPSAVGEVTGDPRLIVPKISVDVPIIWTADAANENSLN
                     AAMDKGVAWFNIQGASAKPGEKGNFALSGHSSNDWLDQGNYKFIFARLEQLGEGDTIY
                     VNYNSTRYVYTVSKKQVVKPTDVSALAVGTDKPRITLITCTPLGTALNRLLVFADQVS
                     PDPNSAIQANTKTTSLGTATQMPSNSPTFLERLFGAH"
     gene            complement(5748..>7026)
                     /locus_tag="VLF64_00875"
     CDS             complement(5748..>7026)
                     /locus_tag="VLF64_00875"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HSW90528.1"
                     /translation="GATVTTDNSELANKTASKIVLSAGDHTVAIGKNGYSTSKKDVIV
                     KPGTVLWLNYALLFPTDPKVSTAAKYTAVVSALPSPNEHTMAVLADGAAPEITLTTLN
                     TDSPVTTKLTVPVSAYTQPADGATQSFSLVEWDKDSHLLLVKHAVNNTQEYISVDTRD
                     ASRAVNVSAGLGVSASVMRYALNDSNVVYMLTTSHELRRGNISSMTLSGPLASAVDDF
                     SVSELNVIMYETLPDANDQRIVGYVSSDSGKAKMLSSYSGLGGTALLASFGSYYGEHY
                     VVILHGTTLDILRGDLPASDSNAVLSLTHYASLTVANGGDHLDFSPDNNRMVYVTKGA
                     RVMTYDLELKDAATTTLQEPLTRDIQWLDGYHMLSANKDGYYYDYDGTNSQKFASNVL
                     DLPAALESGNKYLYYFTKSGAETLLNRVRMTND"
BASE COUNT         1861 a         1966 c         1623 g         1576 t
ORIGIN      
        1 gggccccacg cacccatcta tttcgcgtgc ttcatagcgg cctcacgtgt ctccggccgg
       61 ctcaccacac gtgcccattt gagcggtagc agcacagcga ggataccgag gccaacccct
      121 agcgtccata ccatcacgtc agcccgatag gccatttcca tggccaccat gccgatgtta
      181 atgacgaacc agtagccaaa cacgacgtca gtcacctcgg ccacctttgc cttacctcta
      241 tggatgaaca gctgcaccgc cacaaagaac agggcgccga caccgtagat cagcaacgcc
      301 agcatccacc aatagtcgat gatgaatgcc aaagccgcaa ccgcgagcgc caacatcaga
      361 gcgtccggac cattatccag agcatgatga tcgatgcgcc gccacggcag ccgtgcctcc
      421 tcttcgggcg tgtacggcca acagcgggcg caccatccgt ccagcgcgtc gctaagagca
      481 gccgcaccga acaggatgag ggcaagatgc cactcatgct gcaggatgca gcctggtacg
      541 acaaacgcca gcaccaggcg agcaagcgtt agtaagtctg caatgtatct catgatactc
      601 cttcgtgcta agaactcgcc aaaagtaggt aacgcctaga gcttttatat cacatatgcg
      661 gtatctctac aaacaccata tggcccagat ttttatatgc taaaggcccg ggtgtttccc
      721 cgggccaaat agcaccgtgc caggcggagc gtcagccgag aatcttggtc aacgctccgc
      781 ggaaagtggg agcctgctgc acgaagccgc aatgtgcggc ctccacccag aacatcttga
      841 tgcccggcac tacgtcgtgc caccagccgg aagcatccgg ggtgacgaca tcgttgccgg
      901 caccacagca ctggaggtag gcgacatcga taccggccag cgaggcgcag gccttctcca
      961 ggtcgcagac cttcgtcagc tcgccatcat cgccgactcg aatcatccat cgcagttgct
     1021 ccagccacag cttgctgctg tgaccaccga gctcctgctt ggctcgcccc ttgatgtagg
     1081 cgcgccaggc gacacccgac atcgcctcgc cggcgatctc gcgcatcgtc tcagtgtccg
     1141 ggacctcgat gtagtcgtcc ttcggcagca ctttgacgtg cgccagcggg cggagcaaat
     1201 gcccgaacgg tgaggtgatc acccggcttg cgaagggacc gacggctttc atcgtcttcg
     1261 cgccagaggg tgcgtcgatg atgacgcagc gcaggccact caacacatcc tcgtccggaa
     1321 tcagcaactc gcggatctcg cgaacgacaa acgggaccaa catcccaccg agcgacgcgc
     1381 cgaccagcaa aaccttctta ccgcgacgga ggctgttcag gacgtcgagg gcggcaccac
     1441 gtacagacca gtccggcttg aaacagttcg ggccgaacat cccatactcg gcaactccat
     1501 cagtggggat gccgagagcc tggaagttct cgccccggct caggatgcca ggcagcagaa
     1561 acgctacttc gtcttcacgg agcatgttcc aataccaact ctctaaggag cactgggtat
     1621 acccaggtcg tagcacggtg ctacatagtt atattataac ataatataac tatgtagcaa
     1681 cttcagaaaa tagatgatag gagagaagag cggctacacg acgtaatggg caataagttt
     1741 agccgttggc acacccataa tatcaaatgg aatttcagga ctgcgatcaa taataaccat
     1801 cggccgatcc ttgtagcccc taatctcatc tggcacgtca gtcacgatat accgcggcgc
     1861 accccttggc atcactacag gctgcaccgc acgttcgcta tcggcacggt tacgctcttt
     1921 aatttgcgcc actagcgtat cgacttgctc tttaaaatca ccctcaagct cattatacgg
     1981 ctttggcatc tggccaagcg tacgctgcgc acttactatt ggatcacaaa cgaaaaatag
     2041 tcctaatata tatttacaaa gcccttcacg ctcaagcatc accccaatct cttcaagcgc
     2101 tcggccatcg agcaaaacta tatcaaaccc ttccgcgcgc gcatcgcgaa gccatttacg
     2161 cagcaaatta tctttaaatg tctggctcag cggtcgagca cctactttcg cgctgttcac
     2221 cccaacttca ctagtgtaaa ggagcgattc acgttcgctc ttaggcatgt gatacacgtc
     2281 ttttacaagt tgcacgcatg cagcaatatt cgcatcatct ccaaggtaag catcgactgc
     2341 agcaggtgac ccaatgtcaa cgcccgcgcg agtaattgca actactaaaa cgcgaaacac
     2401 ttgtcccgca tcaataaaca tcacactgta gccttcgacg tcacgaaggt aatctttcac
     2461 caaccccacg acggtgccct taccgctacg ggcttctcca tcaaacgtca ccaacttcgg
     2521 gaggctattt tgcattgtct cttactatac acgcgggtag ggtacggtca acagtaaacg
     2581 aagactttgt tacaatagtt aacatgaaat tatattcttg gaacgtgaat gggattcgtg
     2641 ccgtcttaac aaaaggcgct ttgcaagatt ttattactaa acatgaccca gatatactct
     2701 gtctgcaaga aacaaaagcc gagcgtggac agttcgaaat tgatctccca caatatgagg
     2761 agcatttctt tagcgcagcc aaaaagggct atgcgggtac ggcgattttc agtaaaattc
     2821 cagcacttcg ctatatcgat ggcttccctg agactatcat caaacagtac aacgtaaccg
     2881 gcgatacgta cggcgaccca aataaagaag gccgcatcat tgcagcagaa tttgaagatt
     2941 tttgggttgt tacttgctat acgcccaatt caaagggcga tctctcacga ctatcgcttc
     3001 gctacgattc atgggaccac gcatgcctcg catatttaca gcagctagag caaaccaagc
     3061 cggtattgta ctgtggcgat atgaacgttg cacacgagcc gatcgacctc gcaaatccta
     3121 aagcaaatgt tggcaaacac ggctacacaa acgaggaacg tgaacgtttt gacgactacc
     3181 tagcagccgg atttattgac acctatcgta ctgctaatcc agacaaagta ggagcgtata
     3241 gctggtggac ccactgggca aacgcccgcg cgcgtaacgt tggctggcgc atcgactatt
     3301 ggctcgccag caaagccatt gcaggcaaga taaaaaaccc gcagattcat cctgacgtta
     3361 tgggaagcga tcactgtccg attagcatcg aactatagat ggacattcaa tcatcggaga
     3421 gcattctatg ccatattctc tagctttgcg ctaaactagt aagatgcatg actactggaa
     3481 gcagcagcag ccaacccagc cactcttcac tgatatagag tggagcaagc cggaacaaaa
     3541 agcgagccga ggccgtctcg gtattattgg tggcaataag ctagggtttg ctggcgttgc
     3601 cgagagctat agtacggcac tcaacaccgg tgcgggcgag gtacgtgtac tcctgccgga
     3661 ttgtcttcgc aaaagtattc caccttcaat gactgatgtt atttttgcac cctgtaatcc
     3721 tagcggcagc ttatcgcgcg atgcgatgcc cgatctcacc gcgctcggca actgggcttc
     3781 atgtctattg ctcgcaggcg atgccggccg caacagtgaa acagcaatta cctatagtga
     3841 tcttattcgt aacttttctg gccaactcgt tattacgcgc gacgcggttg acctcgtaaa
     3901 aaatgatgcc gaagcactcg taaatcgccc aaacaccgtg ctcatcgtca gcttcgctca
     3961 attacaaaag atattccagg cagtctatta cccaaagatg ctgacgttca gcatgcaact
     4021 cctccaactt gtcgaagcga tacataaatt taccgttacc tatcctatta cgatcgccgt
     4081 actccaccgc gacacgctca tcgttgcaca taatggagag gttgtcacca caaaatggga
     4141 aacaccgctc gccatttggc gtggcatcac cgcagcacgc gcagccacct acctaatgtg
     4201 gacaccaggc caaccccttc aggctatcgc caccagtctt gtgactaaag agtaatcttg
     4261 gtataattca ggtattgatt actagtcacg tgtgccgcgg tggtggaatc ggtagacacg
     4321 ttagactcaa aatctgatgc ccattagggc gtgccggttc aagtccggct cgcggtacca
     4381 gtgattagta atgaagcata ggcgttttgc acgcatcgag ttatactagg gtaagatata
     4441 tttttattat gcgcccagat gaaaaccgtc cagctacacc tttatcagtg cccgcgcgtc
     4501 agagggcatt aattagtagc agccgcgttc aagacaacaa agaagcggca gcaaacattg
     4561 tacgaaatca gattgatacg atctatcaaa atgacccgag cgccacaacg gaagcaggcg
     4621 ctcagcctac tattacgcct gggatcgccc aactcgaacc agaagctata cagacaaccc
     4681 cagcaaagtc tccgattacc ccaggccctg gcgctattaa tatgcaggct gcaaaccagc
     4741 aagccgaagc aattaacccc tatggacgca cacacgatga gtcggagcat gggacgccaa
     4801 ctgacgcttg gcgcaaatac catagtgctt ggcaaagcta ctatcaacag tactacgaac
     4861 gctactacgt cggtcaagta cacaacgtaa aaagatcgct cgaaaaagac gtaaacgcat
     4921 caaccaatga tgcaccagag acaatctcag gtgacgaagc tatgtatgat ttgcgttcaa
     4981 aactgcgcaa ccagatcaat gaacgtgcac aaaaagtgcg caagagtcgc cattttatcc
     5041 caattatttc agcgctttgt gtcatgctag tatttgtgtt tttacagtac aaccgcttga
     5101 tgtttgcagc aatcgacgct tacatcagcc cgggcagcct tgatccatca acgcttatta
     5161 ttgatccaag tgcggtaggc gaggtaacgg gtgaccctcg gttgattgtg ccaaaaatta
     5221 gtgttgacgt accaatcatt tggaccgccg atgccgcaaa cgaaaattca ctcaatgccg
     5281 caatggacaa aggtgttgct tggtttaaca ttcaaggcgc cagtgccaaa cctggcgaaa
     5341 agggcaactt tgcactctct ggacactcct caaacgactg gctcgaccaa ggtaattaca
     5401 agtttatctt tgcgcgtctc gagcaattgg gtgaaggtga cacgatttac gtcaactaca
     5461 acagcacccg ctacgtttac acggttagca aaaaacaggt cgtgaaacca acggatgtta
     5521 gcgcgcttgc cgtcggcact gacaaaccac gcattacact tatcacctgt acaccacttg
     5581 gcaccgcact caatcgtctg ctcgtcttcg cggatcaagt cagcccggac cctaactctg
     5641 caatacaagc aaacaccaaa accacatcat tgggcactgc aacgcagatg ccatctaact
     5701 caccaacatt ccttgagcga ttgttcggcg cacactaaag tcttacatca atcattagtc
     5761 attcgcacgc gattgagaag cgtttcagca cctgattttg taaagtagta taagtattta
     5821 ttgccgctct ctagcgcggc aggcagatca agcacattgc tcgcaaattt ctggctattg
     5881 gtgccatcgt agtcgtaata gtagccatct ttattagcag ataacatatg atagccatca
     5941 agccattgga tatcccgggt aagcggctct tgcaaggtcg tcgttgctgc atccttaagc
     6001 tcaagatcgt atgtcatgac gcgcgcgcct tttgtgacgt acaccatacg attgttatct
     6061 ggcgagaaat caaggtgatc accaccatta gcaaccgtta acgatgcata gtgggtgagc
     6121 gaaagcaccg cattactatc gctcgctggc aagtcacccc gtaaaatgtc caatgtcgtc
     6181 ccgtgtagaa taacgacgta gtgttcgccg tagtacgacc caaacgaggc aagaagtgcc
     6241 gtgccaccaa gtccactata actcgacaac atctttgcct tgccgctgtc agacgacaca
     6301 tagccaacga tgcgctgatc atttgcatct ggaagtgttt catacataat tacattcaac
     6361 tcgctaacgc tgaaatcatc caccgcactc gcgaggggcc cactcaaagt cattgatgaa
     6421 atattaccac gacgcagttc gtgcgaggta gtgagcatat atacaacatt gctgtcgttc
     6481 aatgcatagc gcatcaccga cgcgctcaca ccaagacctg ccgatacatt aacggcgcgg
     6541 gatgcatcgc gggtgtcaac ggagatatat tcctgcgtat tatttacggc atgttttaca
     6601 agaagcaagt ggctgtcttt atcccattcc accaagctaa agctctgcgt agcaccatca
     6661 gcaggctggg tatatgccga tactggcacc gtaagcttcg ttgtcaccgg gctatccgtg
     6721 ttcaatgttg tcaaagtgat ttctggtgct gccccgtcgg caagcacagc cattgtatgc
     6781 tcgtttggtg atggtaacgc acttacaact gcagtatatt tcgcagccgt actcacctta
     6841 gggtcagtag ggaacagtag ggcgtaatta agccatagca ctgtgcccgg cttcacaatc
     6901 acatcctttt tcgaggtaga gtagccgttt ttgccaattg ccaccgtgtg gtcacctgca
     6961 gaaagcacaa tcttgctcgc tgttttatta gctaactcac tattatctgt tgtaactgtt
     7021 gcaccc
//
LOCUS       DATIIX010000031         3494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_64115, whole genome shotgun sequence.
ACCESSION   DATIIX010000031 DATIIX010000000
VERSION     DATIIX010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64115"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /locus_tag="VLF64_00880"
     CDS             complement(<1..1493)
                     /locus_tag="VLF64_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HSW90529.1"
                     /translation="MARPIVLSNGELHVGINSFGEVHDFYYPYVGLENHAAGRNLRHH
                     VGIWVDGQISWLDDGKWEFDFSYPHDSLVGHTIAKNHELGVMLELDDAADTEFSAFMR
                     NIHVINMQSSPRDVRLFLHQAFDIGDAGSNTDTAQYLPDAKAILHYRGRRAFVISAQT
                     DHKDYFDQYTVGLFGIEGRDGTYRDADDGELEMCSVEHGRVDSTLRFKMELAPHSSAR
                     VHYWIACGTSTREALYIHKQMQGQGVHRRLDATSKWWHEWLKPANEVASKIDPAHREQ
                     FLRSCMIIKSHIDKRGAVVASTDSSMLNYGRDAYAYCWPRDGAYVLWPLIRMGYKDEP
                     YRFFEFCRRALNPGGFLMHKYRADGALGSSWHSYVHEGGVTAPPIQEDETALVLFVFS
                     QFYHTSGNNEVLLKEFYEPMVLPMADFLARYVDGRTGLPKPTYDLWEEQFLTTTYSTA
                     VTYAALMAAADLAEVRKDSNNAVKWRSAAESIRQAAEKHLYNEERKA"
     gene            complement(1494..2717)
                     /locus_tag="VLF64_00885"
     CDS             complement(1494..2717)
                     /locus_tag="VLF64_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HSW90530.1"
                     /translation="MSKRGIVLYLHVHQPWRVRDYTVFDTAENHDYFGPFTESHRNNK
                     EIFEKVANKSYRPMTKLLKELLDTHPDFKVSLSITGTLLEQAEQWGPDVIESFKSLVN
                     TGRCEIVAETYYHSLAFFYSRSEFEHQVEKHRQKIRDTFGVETSVFRNTELSYDNNLA
                     RWADEFGFKGILSEGWDPILGWRSPNYVYRPVGTKNIKLLMKNYRLSDDVAFRFSNRA
                     WEEYPLTADKYDDWVNTSLGDDGEVINLFMDFETFGENIWEDTGIFSFFKDFVGRWLA
                     NPANTFYMASEACDTFEAADEIDTPYTTTWADTERDLSAWLGNGMQHEAMRHLYDLEG
                     DILRTNDEALIGDWRRLTTSDHAYYMCTKYFTDGDVHAYFSPYESPYDAFLYFINALR
                     DVRFRLYEHHGHGDL"
     gene            complement(2714..>3494)
                     /locus_tag="VLF64_00890"
     CDS             complement(2714..>3494)
                     /locus_tag="VLF64_00890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSW90531.1"
                     /translation="AGSFGSGNPIVHEIEYQGLMMADRIIAVSGITKNIIIHRYGIPA
                     DKIEVVHNAIDPNSLGSYGYDGRTYRYLESLRREGYTVVSTVTRFTAQKGLTQLIHGM
                     ARACERYDKLVLLLAGDGEQRDELIGLTAELGISDKVFFTGFVRGKQWRDAYSVADVF
                     VMSSVSEPFGLTALEAAHYGSALIISKQSGVGEVLKSIMRYDFWDTDKLADQLVGIAT
                     SPALSRQLRDDVKYEYARLSWHDVARKCMHLYTRMKIGATV"
BASE COUNT          994 a          910 c          728 g          862 t
ORIGIN      
        1 aaggcttttc gttcttcgtt gtataggtgc ttttcagccg cctgacgaat agattcagct
       61 gcagaacgcc actttactgc attgttgctg tctttacgta cttctgcaag atccgcggcc
      121 gccatcaacg ctgcataggt aacagcggta gaataggtag tagtcaaaaa ttgctcttcc
      181 cataaatcat aggtaggttt aggcaagcca gttcttccat caacataacg cgccaagaaa
      241 tcagccatcg gcaacaccat cggctcataa aattccttca gaagcacttc gttattgcct
      301 gatgtatggt aaaactggct aaacacaaac aacacaagtg cagtttcatc ctcctgaatt
      361 ggaggggcgg ttacgccccc ttcatgcacg tatgaatgcc agcttgagcc taaagcgcca
      421 tctgcgcggt atttgtgcat caagaaccca cccggattaa gtgcccgacg gcaaaactcg
      481 aagaatcgat aaggctcatc tttgtatccc atgcgaatca atggccatag tacataggcg
      541 ccatcacgcg gccaacagta cgcatacgcg tcgcgaccat aattaagcat actgctgtca
      601 gtacttgcaa ccactgcacc gcgtttatca atatgcgact taataatcat acatgagcgc
      661 aagaattgct cacgatgcgc agggtcaatt ttgcttgcca cttcattcgc cggtttaagc
      721 cattcatgcc accatttact cgtagcatca agtcggcgat gcacgccttg accttgcatt
      781 tgcttatgga tgtaaagcgc ctcacgagtt gaagtaccac aggcaatcca gtagtgcacg
      841 cgcgcagagc tatgcggagc tagttccatc ttgaatcgga gtgtcgaatc aacacgacca
      901 tgttccactg agcacatctc aagttcgcca tcgtccgcat cgcgatatgt tccatcacgg
      961 ccttcgattc caaagagccc tacggtgtat tggtcaaagt aatctttatg gtcagtctgc
     1021 gcggatataa caaacgcacg acggcctcga taatgaagga ttgcttttgc atcaggtaga
     1081 tattgagctg tgtcggtatt gctcccagcg tcaccaatat caaatgcctg gtgcaagaac
     1141 agccgcacat cacgcggaga tgattgcata ttgataacat ggatattgcg cataaatgca
     1201 ctgaactcgg tatcagccgc atcatcaagc tctaacatga caccaagctc atggttctta
     1261 gcaatcgtat ggccaactaa tgaatcatgc ggatagctaa aatcaaactc ccatttacca
     1321 tcatcaagcc aagaaatctg accatcaacc catatgccta catggtgtcg caagttacgt
     1381 cctgctgcat ggttttcaag cccaacgtac ggatagtaaa agtcgtgcac ttcaccaaag
     1441 gagttgatac caacatggag ttcgccgttg cttagaacta tgggacgggc catctataaa
     1501 tctccatgcc catgatgctc gtacaaacga aatcgcacat cacgcagggc attaataaag
     1561 tacaagaatg catcatatgg cgactcatac gggctgaagt aagcatgcac gtcaccatcg
     1621 gtgaagtact tcgtacacat gtagtacgca tggtcgctcg tagtgagacg gcgccaatcg
     1681 ccaataagtg cctcatcgtt agtgcgaaga atatcccctt caagatcata caagtgacgc
     1741 attgcttcgt gctgcatacc attaccgagc cacgccgaga ggtcacgctc ggtatcagcc
     1801 caggttgttg tgtaaggggt atcaatctca tctgcagctt caaacgtatc acaggcctca
     1861 cttgccatgt aaaaggtatt cgcaggatta gcaagccatc ggccaacaaa gtccttaaag
     1921 aagctgaaaa taccagtatc ttcccaaatg ttttcaccaa aggtctcgaa gtccatgaat
     1981 aaattaatca cttcgccgtc atctccaagc gaagtattaa cccaatcatc atatttatcg
     2041 gcggtaagcg gatattcttc ccaggcacgg ttactaaaac ggaatgcaac atcgtcactt
     2101 aagcgatagt tcttcatgag caacttaatg tttttcgtgc ccacaggcct atagacgtag
     2161 ttcgggctac gccaaccaag aattggatcc cagccttcag acaaaatgcc cttaaaaccg
     2221 aattcatcag cccatcgcgc aaggttgtta tcgtagctta gctccgtatt acggaatacg
     2281 gacgtttcga caccaaatgt atctcgaatc ttctgacggt gcttctcaac ctggtgctca
     2341 aactcactgc ggctatagaa aaacgcaaga ctatgatagt acgtttcagc aacaatttca
     2401 cagcggccag tattaacgag tgatttaaag ctctcaataa catccgggcc ccattgctca
     2461 gcctgctcaa gtaacgtacc agtaatagaa agcgatacct taaaatcagg atgtgtatca
     2521 agtaattctt taagcaactt cgtcattgga cgataggatt tgttcgcaac cttctcaaag
     2581 atttctttgt tattacgatg gctctcggta aatggcccaa agtaatcgtg attttcagca
     2641 gtgtcgaaca ccgtatagtc acgcacacgc catggctgat gcacgtgtaa gtaaagaact
     2701 ataccacgct tgctcatacg gttgctccga tcttcattct ggtatataga tgcatacact
     2761 tacgggcaac atcatgccag ctaaggcgag catattcata cttcacatca tcacgaagtt
     2821 gccgcgagag tgcaggggag gttgcaatac caacaagctg gtcagccagt ttatctgtat
     2881 cccaaaaatc atagcgcata atactcttaa gcacttcacc aacacccgat tgtttgctaa
     2941 tgataagcgc gctgccgtag tgcgcagctt cgagtgccgt caaaccaaac ggctcactca
     3001 cgctgctcat aacaaataca tcagcgacac tgtaggcatc gcgccactgt ttgcctcgta
     3061 caaatcccgt aaagaacacc ttatcagaaa tcccaagctc ggcagtgagc ccgataagct
     3121 catcacgctg ctctccatca ccagcaagta acaatacgag cttatcgtaa cgctcacagg
     3181 cacgcgccat gccatgtata agttgcgtaa gacctttttg cgctgtaaaa cgcgtaaccg
     3241 tcgacactac cgtataacct tcgcggcgaa gtgattcaag gtagcgatac gtacgaccat
     3301 catagccata actaccgagt gagtttggat caattgcgtt gtgcaccacc tcgatcttat
     3361 cggccgggat accgtaccgg tgaataataa tattcttcgt aataccactt accgcaataa
     3421 tacgatcagc catcataaga ccttggtatt caatctcatg aacaataggg ttgccgctac
     3481 caaacgaacc agca
//
LOCUS       DATIIX010000032        24453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_397088, whole genome shotgun sequence.
ACCESSION   DATIIX010000032 DATIIX010000000
VERSION     DATIIX010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 24453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24453
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397088"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VLF64_00895"
     CDS             complement(<1..480)
                     /locus_tag="VLF64_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HSW90532.1"
                     /translation="MKQRLDQLLVQRGLVPTRSQAESWIKLGKVTVNGKVAAKSGYFV
                     HDTAKIVLTAEEQYVSRAGLKLASVAGKFTIDFIGTTVLDVGSSTGGFTDYALRHGAA
                     KVYAVDVGTDQLHPSLRGDERIELHEKTDIRDFPPATPPDIVVMDVSLISVRQILQYI
                     "
     gene            complement(488..1054)
                     /gene="efp"
                     /locus_tag="VLF64_00900"
     CDS             complement(488..1054)
                     /gene="efp"
                     /locus_tag="VLF64_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HSW90533.1"
                     /translation="MFMYQPTDLKKGVVCQIEGQPFRVIEYNQKVMGRGGSIVNVKLK
                     NLITGAVIPKTFKGQEKVEAAEVSSRTVQYLYTDGETYFFMDPETFEQFELSADLMDE
                     AKGFLKEGENLSLQFFSERVINVELPKNIYLEVTYTEDVVKGDTTSNVLKDATLETGV
                     TVKVPAFIKTGDIISVDTATGEYRERKK"
     gene            complement(1222..1422)
                     /gene="rpsR"
                     /locus_tag="VLF64_00905"
     CDS             complement(1222..1422)
                     /gene="rpsR"
                     /locus_tag="VLF64_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HSW90534.1"
                     /translation="MIKRFKKDTPVYFDYRDVKTLSRYINIYGQIEPVAKTALSAKQQ
                     RQLAVAIKRARHLALMPFVSHG"
     gene            complement(1435..1884)
                     /gene="ssb"
                     /locus_tag="VLF64_00910"
     CDS             complement(1435..1884)
                     /gene="ssb"
                     /locus_tag="VLF64_00910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HSW90535.1"
                     /translation="MAKGFNKVILMGNLTRDVEMRTTTGGQNVANFSLAVTRSWKGQD
                     GQMQDQTSFINCVAWGKPGEIIAQYVQKGAPLLVSGRLDQRSYQDKDGNNRQAVEVVV
                     EDFNFVGGGRGDDSGTPARSSSSSSKSNDVVIEDIDDKPIDLSEIPF"
     gene            complement(1908..2264)
                     /gene="rpsF"
                     /locus_tag="VLF64_00915"
     CDS             complement(1908..2264)
                     /gene="rpsF"
                     /locus_tag="VLF64_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HSW90536.1"
                     /translation="MKEYELTVLIHPDLEVDLEAPLAKVRKLVTDNGGEIVSEDNWGK
                     KKMAYRIKGEEFAVYVAFDVTLPATAPLKISNTLNITEEVLRYLLVTVDEKGRALLEE
                     AKKRAAENGDDDTSEE"
     gene            complement(2364..2975)
                     /locus_tag="VLF64_00920"
     CDS             complement(2364..2975)
                     /locus_tag="VLF64_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90537.1"
                     /translation="MIQTELPTKHTKIENSEQVAAETDLRDFTRQELPLARELGAKVC
                     ASVQELWELYSARRYGSTIHVGRRIVEVEGVAMRDYGRRQLEETIVRSRVYYDGNKSD
                     FGPSEMLSVHIKTDDGEELITVDAAGDAVVQYSENAYPEDWLPMSFERAEGTVDELSH
                     RAVIAAMRHGARTEEEKAVANAHAKALLHQRFPLFARTETSLE"
     gene            3057..3860
                     /locus_tag="VLF64_00925"
     CDS             3057..3860
                     /locus_tag="VLF64_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HSW90538.1"
                     /translation="MIVAIQGQAGSFHEQAAQQWYGASIDILPCTTFGDVFDAYANGD
                     ADAIVTAVENTLYGSINQVYQYIEDCEAPIVGEIKLAIKQMLIGLPGTKLSDITEVYS
                     HPVALAQCRKWLAKHLPRAELIEFFDTAGAVEYVKNTGEKHMAAIAGEQAAELYKTAI
                     IQPSIQDNPKNITRFLVLEPSLAETVSRSSLVITTSHKPGALVEVLQVFADANINLVK
                     LQSQPIIDQPWHYKFFMVVDCAGEQLYKLVERIEQNDHQVTLLGEYKAA"
     gene            complement(3987..6224)
                     /locus_tag="VLF64_00930"
     CDS             complement(3987..6224)
                     /locus_tag="VLF64_00930"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HSW90539.1"
                     /translation="MAKKRKTTRGRKTKKIDSPQHELPSGFWRQVSAILLIALSLLLI
                     VSWFGGGGQLLNWMRDFFLSVFGSATYIVPFIAIYVAVQALKAENNRLPLMTKLSAVL
                     IVVWMSGLTGLFRRVSEPSTGGATGDGLNNVMLALVNMPIAIFVYLLFIVITGIFFLR
                     VSFRDVGYFLAWLIGRAESSEDAANINVMRKNASSDIKESGPLSADFKLNAGVPMMDS
                     EDQKSARLSSLKNSVKQDKEAEEKVAMLAVSDPNWKFPDLELLEKKQSPADAGDIKQN
                     AHVIQDTLREFSIDVEMEGANIGPKVTQYTLKPPAGIKLSRITALETNIALNLAASSL
                     RIEAPIPGQKAVGIEVPNKRAADVRIRGILDSNEWRRTDEPLGFAIGKDIAGAAVVGE
                     LNKMPHLLVAGQTGSGKSVMINTLLCSLLYRNAPSDMKLILVDPKQVEMAPYQDIPHL
                     LTPVIVDPEKTVSALKWAVNEMERRYTLLAEERVRDIKTYNQKVKNKSVSVPDEDGNM
                     QQVDEGTMPYIVIVIDELADLMMVAARDVEALIVRIAQKARAVGIHLVLATQRPDVTV
                     ITGLIKANVPARIAFTVASQIDSRTILDQVGAEKLLGQGDMLMKTASMPKPKRIQGAW
                     VMDEEVLKITDHLRLQSPPQYNEEIISQPVQLNGKGGVVMDFDNEGGDSMFRDACRVV
                     IETRKASTSLLQRKLRIGYARAARIIEEMEEQGIIGPADGARPRDVLIHSLDEIDGGG
                     ADVEV"
     gene            complement(6319..8463)
                     /locus_tag="VLF64_00935"
     CDS             complement(6319..8463)
                     /locus_tag="VLF64_00935"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HSW90540.1"
                     /translation="MGQRRLAKPNADDSSKRPNSQLKPKSNNSVLNATTTRKGEVFRA
                     QRRISEGVNMQASQHIINVPVNKSRFNGYDGKQFSLSMYPKKPRSARPTLKIIPIGGV
                     GEMGIGKNMTAIEYDNDIIVIDMGFLFPGSDYPGINYITPDTTWLEENKHRIRAHVFT
                     HGHLDHIGAFRHIAPKLPAPVFASKFTLGMLQRTMEETDGSFTPDYRELNPEAHDIVQ
                     VCDSFSIELVRVNHSIPDATAVVVRTPVGNILASGDWRIEENPVDGKKFDFPRLSEIQ
                     QKEGFLVFLNESTNCESDGTHTHGEFDIQHSMGEVMDKYPNSRVIISSFSSQLHRMQL
                     ILDEAQKHGRKVAFAGFSMIQNLEVALRTGAIKVPKDTVMKMEDIIKLPDNKITIVCT
                     GSQGEFSAVLNRMATGAHKYIKIKGSDVVVFSSNPIPGNEKYVVRTVDGLMREGGEII
                     QNGKTHLTGIGPLHLSGHGYYEDHIRVIGAVNPTYYIPYHGEFHMLVHNAELAEKECG
                     IPRDNIFVCDAGDILEFDHEGARKIGRVHVGGIMYDDAGEIVSEVVLKDRIHMSQEGM
                     FVVVLTVQRGSGRLLTSPDIISRGFIYLRDSEELMGMIRAYIKQKAARAFAGKYDIEV
                     IKKEIKDEVTHILYDQTRRTPIVIPVINEIGMNNNRQQGAKPNANIANDGDQSQPQRQ
                     GMMAVRRRQFPPRQVPDSEVQVPREPREPATL"
     gene            8693..10078
                     /locus_tag="VLF64_00940"
     CDS             8693..10078
                     /locus_tag="VLF64_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSW90541.1"
                     /translation="MNIISRGIRNAFRNIVRSGSIIIILALSIGLVIAMLAARQAVQD
                     KIASVKANTGNTITVSPAGVRGFDGGGNPLTSDQLAKVANISHVTKVVETLNDRLTSS
                     GTSLKSGIDPGSLGRRNASNSGIGEGSAVFRVDDSNSDSNAVHTFSIPVTITGTNNES
                     AIKTAGTTSLSWKSGKMFDTTKDANVAVIGAQIASKNNLSVSSTFTAYNTTFTVVGIY
                     DAGNTFNNDGVYIPLPTLQKLSDQSGDITSATVTIDSSDNLASVTNTIKSQLGSAADV
                     TNSADIATATTAPLESVASIALFSLIGAVIAGAVIILLTMIMVVRERRREIGVMKAIG
                     ASNIVIMWQFIVEAITLTVLALIVGTGIGVVAATPLTNVLISNDSTSSTNSDPSITQV
                     GGGRRMMRFAGPGQFGQASRKTIENIQASVGGATLTEGAALALVIAILGSAAPSLMIS
                     KIKPADAMRNE"
     gene            10078..10752
                     /locus_tag="VLF64_00945"
     CDS             10078..10752
                     /locus_tag="VLF64_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSW90542.1"
                     /translation="MLVVKDVTKQFKQGDNTVTALNKLSFTVNDGEFVSIIGKSGAGK
                     STLLGVLGALDTPTSGSIEIGDTDIAKLSDHKLIGYRNTSIGFVFQSYNLIPNLTAVE
                     NVMLPMEFAHIPSKERRDRAIQLLEEVGITDGREGRKPGKLSGGEQQRVSIARALTNH
                     PKLILADEPTGNLDSENGKMIFDLLHKLSRSENTTVVVVTHDLDIAGKTDRTFTLSDG
                     KLVTKK"
     gene            complement(10819..11391)
                     /locus_tag="VLF64_00950"
     CDS             complement(10819..11391)
                     /locus_tag="VLF64_00950"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HSW90543.1"
                     /translation="MTKQEQMDELRERLIAADVTPELRKTAIQLVMGDGNLDAEVVFI
                     GEAPGKNEDEQGIPFVGAAGKFLNEMLGAAGMQRSDIYITNIVKYRPPNNRDPLPDEK
                     KAFWPYLLKQLQIIKPKVVVTLGRHSMEYFLPGMYISKIHGEPKRIKFGDDTIIIVPL
                     YHPAAALYNGSLRETLLDDFRRIPEIIKKI"
     gene            complement(11393..12331)
                     /locus_tag="VLF64_00955"
     CDS             complement(11393..12331)
                     /locus_tag="VLF64_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90544.1"
                     /translation="MNDQPQTPTSTMQDKPEGVIGPSATEGLTPTPSITGQQSPIPNL
                     SPNKSKKTRTVLFVIVGGLLVLGIAVAVVYFAFFYVSKADYKSAEKQTNTAIAAYHKA
                     DDVAEEWSTAAVDTSATDRDIATKKAAYNDANTAYKTTISSLSGMRALKDDKVKSAYD
                     AFVTKNNAAMVNNDSMAEAMPTLRTMVVECNNADTTGMDTSDLSTIADSWDRAFTKCT
                     KAIKELSSARNVDVATIGKKLEQYFTEMRTDIVALQVAYIAKDETAFTKAYNAFTDVE
                     DKYNADASLKNVKKHQDSLLPETELNNLLAAIRSRE"
     gene            complement(12412..14532)
                     /gene="pnp"
                     /locus_tag="VLF64_00960"
     CDS             complement(12412..14532)
                     /gene="pnp"
                     /locus_tag="VLF64_00960"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HSW90545.1"
                     /translation="MSIINPSGKEIFAVTTELCGRPLTLEVNRVGFRSTGSVLVTYGD
                     TVVLGTATVGTRPVVLDYFPLSIEYQEKFYAAGKISGSRFIKREGRPSDDAVLIGRMI
                     DRPIRPLFPKGYRQEVQVVSSVLSMDPNFRPDAIAMIAASAALMLTGTPFDGPVAGLR
                     IGRVNGEFKAFLTPEEREQSDLDLVVAGIESGITMVEAGAEEVSEEVIIDAMAWAYEA
                     FQPAIALQKELKEKVAPKEQEYTLVLPDTTIQAEADEWVEGKIGATIRRPYPERNEMV
                     NEIRLAFHIAMAKKHGGEEEYAGIRSQYDEAFTMALHKDVRKGIVEDNARPDGRKLEE
                     VRPLSSEIGVLPRAHGSSIFTRGVTQGMNIVTLAPLSFAQTVDTMEQTDYERRYMHHY
                     NALPFTVGEVGRMGSPGRREIGHGYLAERALTAVLPTEEEFPYAIRSVTEIMSQNGST
                     SMAATCSSCLALMDAGVPLKRPVSGIAMGLMMDGDTPYVLSDIADAEDFAGDMDFKIT
                     GTEKGVTAMQMDMKVHGLPVEVLRKAVEQSKPGRAHILEHMLTTIATPRPELSPYAPR
                     IEKIKINPDKIGAVIGKGGEVINKITSETGAMIDIKDDGLITVASPDGESIERALEWI
                     RGLTEEPEVGRIYTGKVVSIKDFGAFVNILPGTDGMLHISQLSDKRVEKVTDVLKEGQ
                     MVKVKLDAIDEKGRLSLTMKGIEQ"
     gene            complement(14956..15426)
                     /locus_tag="VLF64_00965"
     CDS             complement(14956..15426)
                     /locus_tag="VLF64_00965"
                     /inference="COORDINATES: protein motif:HMM:NF027885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FBP domain-containing protein"
                     /protein_id="HSW90546.1"
                     /translation="MHKITREEFVERVASARIKPRLRKTLRFVPEEVTEWDSRDFLAV
                     TDKSGNEGVLLYQDTTIPFTLSPRVARTGGRVAAIICDICATWRRGPESAVLTLPKGD
                     NRTVSYLVCADLNCSLHVRGMTDAGILSRSQIHEDITPDRRQERLRERMRDIVA"
     gene            complement(15419..16015)
                     /locus_tag="VLF64_00970"
     CDS             complement(15419..16015)
                     /locus_tag="VLF64_00970"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HSW90547.1"
                     /translation="MKELIFATTNNGKVVSLQAHLDRAGCDIIVIAKQLEITEIQADT
                     ALEIAKVKAVEAFKQLGHPVVVDDSEFCIEALGGFPGPYQKYMGKKIGAEGLVKLMQG
                     YDNRRAYFISNLVFVDENGEQYAFSDEPYWGMIADAVDEVERPEAWGPLWRVFIPEGR
                     SVTLSQSRPEEHHQMNDKRSAKGAYKKFALWLKEREDA"
     gene            complement(16066..16332)
                     /gene="rpsO"
                     /locus_tag="VLF64_00975"
     CDS             complement(16066..16332)
                     /gene="rpsO"
                     /locus_tag="VLF64_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HSW90548.1"
                     /translation="MISKDDKAKAIALTQVNKNDVGSPQAQVSVLTARIKEVTEHLKA
                     NKHDHMARRGLIQMVGRRKRLLKYLETKDFEAYKATLEKLGLRK"
     gene            complement(16359..17021)
                     /gene="truB"
                     /locus_tag="VLF64_00980"
     CDS             complement(16359..17021)
                     /gene="truB"
                     /locus_tag="VLF64_00980"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HSW90549.1"
                     /translation="MMDGEIILIDKPAGMTSFGVVARVRRILSRRAGKKVKVGHCGTL
                     DPFATGLLILVSGKMTRKAMEFTKLDKVYEAEFILGQESTTGDPEGELTQVSDSVPTK
                     EQVEGALAAFRGEIMQRPPIFSAIKINGQRAYKLARDGKEVEIPERTITVYNLELIDY
                     SYPSLTIRTHVSSGTYIRSLAVDIGMRLGTGAYCKELRRTKIADFSVTDAKALSDFGV
                     EN"
     gene            17068..18222
                     /locus_tag="VLF64_00985"
     CDS             17068..18222
                     /locus_tag="VLF64_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSW90550.1"
                     /translation="MNDKNVWTALHDDYKQRDWAKIPSMFAETSIAYFPQQGRVLDLG
                     AGLGQDSRFFAEKGYDVVSTDLENGTLEEQFADLPSELKNKLSTARVDLREELPFGNG
                     SFDVVYAHLSLHYFDYETTVRLIGEIQRVLRPGGVIAFFVNSTSDPEYASGTKIENDF
                     FQVGDATKRYFSIETTREFTKYIDTTLLDDKGETYKDAAKGVHNLIRFIGTRRQKEAF
                     SMALPYCGAIVEREQNGEKELLMQTRWKPHADPVYSGTIEFPASTLDKPFEDIHDTLA
                     REIKEETGLTLKNIRGDERTSDITSGRDDQIIGFRPFCCTQQIKNGKPWIGFVFVCEV
                     EAGEPKAQHGEAKDAHWMKAGDLRQLYENEPEKLFGLELPAWQYYFDSEK"
     gene            18225..18905
                     /locus_tag="VLF64_00990"
     CDS             18225..18905
                     /locus_tag="VLF64_00990"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HSW90551.1"
                     /translation="MKKVLFGIFAHPDDEAFGPGGTLIKAVHDGVELHLITLTLGQNG
                     TNPDNAPDLGTVRREEWLKSGQMIGATSQHELGYIDGRLCNIEMLEITEKIQHIVRET
                     IADRDIEVEFIGFDLNGLTGHIDHIVASRALCLAFYRLKQDDSRFTRIRLFCLPREQN
                     QSVNTNWIYMEAGRTPEEIDETIDARKYYKKIIDVMRTHHSQRSDAETQIDTLGHGIG
                     INHFIILS"
     gene            complement(18908..20683)
                     /locus_tag="VLF64_00995"
     CDS             complement(18908..20683)
                     /locus_tag="VLF64_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90552.1"
                     /translation="MADQGALKQIIQKLKDSNNVLVTVNSNPSVDELSAALAITLCIN
                     KLNKHATAVFSGNVPPAIEFLNPEKTFENTVDSLRDFIIALDKEKADHLRYKVVDDMV
                     KIFITPYRTTISEKDLEFSQGDYNVEFVLAVGVQNETDLDRALESHGRILHDATVAAV
                     SLGEPTKLGGIEWADSEASSYSEMLFQIAEGVKGEKTLLDEQVATAFLTGIVAATDRF
                     SNNKTSSKVMTVAAQLMSAGANQQLIATKLEEDMAISTHANVAHTSIGEKPTDLQKEV
                     QKDDNGAIGALSISHAPEGDVDTVAREIATGNSSPTISRAEQELAKQAERVNETRQEA
                     ATEAAEQKLAEQLATTVSAAPPVFELPAVAPSTVAPVAQESSLTAHTDEQTGVPSFGG
                     TLNATTEQAEEDKRQQLEDDRNHTILSHNNSYATGVPAFQSPINAAAGQIDAEPEVRD
                     VLAEESGTAPTGHADIIQPPVPFVSSEPTLAEIDAQNRAAHNDARAALDEALNTQEQP
                     APEMPAYEPAPIPAPTAEVAPALPPLPPLPPMPDFSTLPPLPGDPAPVNVASVPDTLG
                     AVLPPAPAEQPPQSIDPAQFKIPGQ"
     gene            20851..21225
                     /gene="rpsT"
                     /locus_tag="VLF64_01000"
     CDS             20851..21225
                     /gene="rpsT"
                     /locus_tag="VLF64_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HSW90553.1"
                     /translation="MPIIKSAIKRMKQTAKRRARNVSIKRDIKGAVKAFTAEPTAANL
                     SAAQSELDTAVKKGLIKKATASRRKAGLAKAAKAVGAKPVAAPKAKTAAKSAAKKAPA
                     KKPATKTTTTKKTAAKATTKKA"
     gene            complement(21270..22208)
                     /locus_tag="VLF64_01005"
     CDS             complement(21270..22208)
                     /locus_tag="VLF64_01005"
                     /inference="COORDINATES: protein motif:HMM:NF044544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90554.1"
                     /translation="MIYLIHGENAYLQKVELQKIARAVGVAPEHIDTDELSLNALADI
                     VRGGSLFAVSRIIVIRELSRDMTLFGKLAEWAEQVPDETTIVLLERKLDKRTKAYKAI
                     IRTATVVTADSFTERDTAAVEEWLRTAAKQYNVSLSLTQVTQMVERALVVSDKVSARV
                     VDQMQLMHAVRALAGTKEVTNDMIATILPQTMVDSVFDMLDIAARRELARLDTLLTDM
                     ARTDDGHRALALLNSQWAQLAAVATLGGSSQSIPTELGLHPFVAKKMQETASRFRSSE
                     VRTLTQFIADLDIETKTSQAGPWDAIYRFLYAITSR"
     gene            complement(22242..23120)
                     /gene="mutM"
                     /locus_tag="VLF64_01010"
     CDS             complement(22242..23120)
                     /gene="mutM"
                     /locus_tag="VLF64_01010"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HSW90555.1"
                     /translation="MPELPEVETVRRGLERLVVGRRVKAIMVHDSPKSFPNNWDDVEQ
                     FAYGATIVAVRRRAKVLLIELSTNYTFVTHLKMTGQLVYVGSERFGAGHPTDSLVGNL
                     PDRSTRVVIAFHDGTMLFFNDQRKFGWMKLYPTPEVMNIDFMQKVGPEPLEDSFTADM
                     FILRLRRRNNTTIKAALLDQTVLAGVGNIYADESLWGAKIHPATRVKDVSDEQLVILL
                     SELKYVLNLAIEKGGSTDKNYVNAEGKRGSYIDFARVFRREGKECPRHPGTIIIKMKV
                     AGRGTHICPLCQPTPL"
     gene            complement(23124..23702)
                     /locus_tag="VLF64_01015"
     CDS             complement(23124..23702)
                     /locus_tag="VLF64_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HSW90556.1"
                     /translation="MIRGIIFDCFGVLTTDGWLQFRGEHFKEGTREYREISQLNHQAD
                     VGYITHDEFMQSIADLSHIDVNDVKTTLEQRSPNNELFDYIRDALKPNYKIGLLSNAS
                     DDWTNELFRPDQVALFDEKVFSFQIGVDKPHAAMYETAAARLGLLPEECIFIDDREGF
                     VTGAKDVGMHAFVFETNAGIKQRIDEIVAQAS"
     gene            complement(23724..24329)
                     /locus_tag="VLF64_01020"
     CDS             complement(23724..24329)
                     /locus_tag="VLF64_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90557.1"
                     /translation="MYRGSRPRILPIIVVIVIVALVVAAIVTVGRMLFSGSGDTRDNK
                     KTTSSVIEQAVLAQDSDRSVRWTVRGPIVADEKFRSYQIIISPSTRTFVTYSGYLDQV
                     IDTKTYGNNLKAYEQFVYALDKTDIAKARDTKDADLRGVCATNGLAYEFETLVNDDPD
                     HTMWSSTCKDSQGTMTADPLQVQALFVNQVPDFRPLFTKIY"
BASE COUNT         6604 a         6323 c         5075 g         6451 t
ORIGIN      
        1 aatatattgc aaaatctggc gtacactaat caacgataca tccatcacta caatatctgg
       61 cggagtcgca ggggggaaat cacgaatatc agtcttttca tgcagctcaa tacgctcatc
      121 cccacgcagt gaaggatgca gctgatctgt tccgacatcc acagcgtata ctttcgccgc
      181 cccatgacga agcgcatagt cagtaaatcc ccccgtcgat gaaccaacat caagcaccgt
      241 cgtaccaata aaatcaatag tgaacttacc cgccacactt gcaagcttca atccagcacg
      301 gctcacgtac tgctcctcag ctgtcagcac aatctttgcg gtatcatgca caaaataccc
      361 ggattttgca gccaccttac cattaactgt tactttacca agcttaatcc aactttccgc
      421 ctgagacctt gttggcacaa gtccacgttg aacgagtaat tgatcgagcc gctgcttcac
      481 ctagacccta ctttttacgt tcgcgatatt cgccagttgc agtatcaaca gagataatat
      541 caccagtctt aataaacgca ggaactttca cagtcacacc agtctcaagc gttgcgtcct
      601 tcaatacgtt gctcgtagtg tcacccttta cgacatcttc tgtatatgtt acttcaagat
      661 aaatattctt aggaagctca acgttaatca cgcgctcact aaagaactgc aggctaaggt
      721 tctcaccctc tttgaggaat ccctttgctt cgtccatcaa atcagcagaa agctcaaact
      781 gttcgaatgt ctcaggatcc ataaagaagt aggtttcacc atcagtgtag aggtactgta
      841 cggtacggct acttacttca gccgcttcga ccttttcctg acccttaaat gtctttggga
      901 tcaccgcgcc agtaattaag ttcttgagtt ttacattcac aatagaaccg ccacgtccca
      961 ttaccttctg gttatattca atcacgcgaa aaggctgacc ttcaatttgg caaacaacac
     1021 cttttttcaa atcagttggt tggtacataa acattattcc tttactttgt tttcaagtgt
     1081 cgtatagaaa atcacggggt gagagatgct atgcggcgca gatcaaggcg cagatgagac
     1141 tcggacctaa caatataaac atattgttag gaagagccgt aatctgcaca acgccgatat
     1201 gcaccgcgga gcgctctcac actagccgtg tgatacgaaa ggcatcaaag ccaggtgacg
     1261 cgcacgctta atagctactg caagctgacg ctgttgcttg gcgctaagag cggtctttgc
     1321 aactggctca atttggccgt agatattaat gtaacgtgaa agtgtcttta catcacggta
     1381 gtcaaagtat actggagtat ccttcttaaa tcgctttatc atattaacat tctcctagaa
     1441 tggaatctca gacaagtcga ttggcttgtc atcgatatct tcaattacta catcgtttga
     1501 tttgctactt gatgaactcg agcgagctgg cgtgccagaa tcatcgccac gaccaccacc
     1561 gacaaaatta aagtcctcaa cgacaacttc aactgcctga cggttgttac cgtccttgtc
     1621 ttggtagcta cgctggtcaa gacgaccact tacaagaagt ggcgcaccct tctgaacgta
     1681 ctgcgcaata atttcgcctg gcttacccca tgcaacacag ttgataaagc tcgtctggtc
     1741 ttgcatttga ccatcctggc ctttccagct gcgcgttact gcgagactga aattagcaac
     1801 gttctgaccg cctgtcgtcg ttcgcatttc gacatcgcgc gtcaggttac ccatcaaaat
     1861 taccttatta aaccccttag ccatactagg cctcctccac tttgttacta ctcttctgaa
     1921 gtatcgtcgt ctccgttttc agcagcgcgc ttctttgctt cttcaagcaa tgcacgaccc
     1981 ttctcatcaa cagtaacgag caggtaacga agtacttctt cagtgatatt tagcgtgttg
     2041 ctaatcttga gtggcgctgt tgctggtaac gtaacatcaa atgcaacata tacagcgaac
     2101 tcctcgcctt tgatgcggta agccattttc ttcttacccc agttgtcttc agaaacgatc
     2161 tcaccaccat tgtctgtgac tagtttacgc actttcgcaa gtggcgcttc cagatcaact
     2221 tcaagatctg gatgaataag aacggttagc tcatattcct tcataccgta aaacctcctt
     2281 tgggatccgt acggatggtt accttatctc aggcaacctt aggttagtga caggtatatt
     2341 ataccccaaa gtttacaaaa aagctattct aaagaagttt cagtcctcgc aaacaaggga
     2401 aaccgttgat ggagaagtgc ttttgcatgt gcattcgcga ctgctttctc ttcctcggtt
     2461 cgcgccccat gacgcatcgc agcgatcaca gctctgtgag aaagctcgtc cacagtgcct
     2521 tctgctcgct cgaacgacat agggagccag tcctccggat aagcgttttc actatactgt
     2581 acgaccgcgt cgcctgcagc gtcaactgta atcagttcct cgccgtcatc agtcttaatg
     2641 tgcaccgaca acatctctga tggtccaaaa tcactcttat tcccatcgta gtaaacacgt
     2701 gaacgtacta ttgtttcttc aagttggcgc cgaccatagt cacgcatcgc aacaccttct
     2761 acttcaacaa tacgtcgtcc gacatgaatc gtactaccat agcgtctagc tgaatataac
     2821 tcccacaact cctgtacact cgcacacacc ttcgcaccca actcccgtgc aagcggcaat
     2881 tcttgccttg tgaagtcgcg taaatcagtc tctgctgcaa cctgctccga gttctctatc
     2941 ttggtatgtt ttgtcggtaa ttctgtctgt atcatacgtc cgcctttcgc gcctaattac
     3001 acttgtttta ctctcccgaa tgcacgatgt caagctaagt gctacaattg tttgtgatga
     3061 tagtagcaat tcagggtcag gccggctcat ttcacgagca agctgcgcag caatggtatg
     3121 gtgcttccat tgacatcctt ccttgcacca ccttcggcga cgtttttgat gcatatgcca
     3181 atggcgacgc cgacgccatc gtcacggcag ttgaaaacac cctctacggt agtattaacc
     3241 aggtatacca atatattgaa gattgcgaag cgccaatcgt cggtgaaata aaactcgcta
     3301 tcaaacaaat gttgattggt ttgcctggca caaaactcag tgatattact gaggtatatt
     3361 cacatcctgt tgcgctcgcc caatgcagaa aatggctcgc gaagcacctg cctcgtgctg
     3421 agcttattga attttttgat actgctggtg cggttgaata cgttaaaaac actggcgaaa
     3481 agcatatggc tgctattgct ggcgagcaag ctgcagaact ctataaaacc gcaatcattc
     3541 agccgtcaat ccaagacaac cctaagaata tcacacgatt cttagtactt gagccaagcc
     3601 ttgctgaaac ggtctcacgc tcatcgcttg tgattacaac aagccacaaa cctggcgcac
     3661 ttgtcgaagt cttacaggta tttgctgatg caaatatcaa ccttgtaaaa ctccaatcgc
     3721 agccaatcat cgaccagcca tggcattata aattctttat ggtcgttgat tgtgccggcg
     3781 aacaactcta caagctcgtc gagcgaatcg aacaaaacga tcatcaagtt accttgcttg
     3841 gtgaatacaa ggctgcgtag caattaaaat agactccgct tggccatctt cgtaagcgaa
     3901 gtgcaaccaa agagcctcca taaggagact ctttgcgtgt aacggggttt tgaatgaaga
     3961 tgggcgaaaa ggagttataa cttaccctac acctcaacat cagccccacc accatcaatc
     4021 tcatccaaac tatgaatcag cacatcgcgt ggtcgagcgc catccgccgg gccaataatt
     4081 ccctgctctt ccatctcttc gataatacgc gcagcacgcg catacccaat gcgcaactta
     4141 cgctgtaata aactggtgct tgccttacgt gtctcaataa caacgcggca cgcgtcacgg
     4201 aacatactat cgccaccttc gttatcaaag tccatcacaa caccgccctt gccgttcagc
     4261 tgaacaggct gagaaataat ttcctcgttg tactgtggcg gcgattgaag acgcagatgg
     4321 tcggtaatct ttaagacctc ttcatccatt acccacgcac cctggatacg ctttggcttt
     4381 ggcatactcg ctgttttcat caacatgtca ccttggccaa gaagcttctc agcgcccacc
     4441 tgatcaagaa tggtacgact atcaatctgg cttgcaaccg taaaggcaat acgcgctggc
     4501 acattagcct taatcaaacc agtaatcacc gttacatccg gacgctgcgt tgcaagcacc
     4561 aggtgaatac caacagcacg cgccttctga gcgatacgca caatcaatgc ctcaacgtca
     4621 cgcgcagcta ccatcataag gtccgccaac tcgtcgatca caatcacaat atatggcatt
     4681 gtgccttcat caacttgctg catattacca tcttcatcgg gtacgcttac tgacttattt
     4741 tttacctttt ggttgtaggt cttaatgtca cgaactcgct cctcagcaag gagagtatag
     4801 cgacgctcca tctcgttcac cgcccattta agcgcactta cggtcttttc aggatcaaca
     4861 atcactggcg tcagcagatg cggaatatcc tgataaggcg ccatttccac ttgcttaggg
     4921 tcaaccaaaa ttaacttcat atcacttggt gcattacgat atagcaaact acaaagcaac
     4981 gtattaatca tcactgactt accgctaccc gtctgacccg caaccaacaa gtgaggcatc
     5041 ttgttcaatt caccaacaac ggcagcacca gcaatatcct taccaatcgc aaacccaagt
     5101 ggctcatcgg tcctgcgcca ttcattacta tcgaggatgc cgcgaatacg cacgtctgcc
     5161 gcgcgtttat ttggcacctc aatacctacc gctttctggc caggaattgg tgcttcgata
     5221 cgaagactac tcgccgcaag gtttaatgca atattagtct cgagtgcagt gatgcggctt
     5281 aacttaatgc ctgcaggtgg cttcaaagtg tactgcgtta ccttcgggcc aatattagcg
     5341 ccctccattt ctacatcaat actaaactcg cgaagtgtgt cctggataac atgggcgttc
     5401 tgcttgatat cacctgcatc agctggagat tgcttctttt caagcaactc aagatctggg
     5461 aacttccagt tagggtcact aacagcaagc attgctactt tttcttcagc ttctttatcc
     5521 tgtttgacgc tattcttaag actactcaac ctagctgatt tttgatcctc ggagtccatc
     5581 ataggtacac cggcgttcag tttgaaatca gcggaaagtg ggccgctttc ttttatatca
     5641 gaagaggcat ttttacgcat cacattgatg ttcgcggcat cctcactact ctccgcacgt
     5701 ccgatgagcc acgcaaggaa ataaccaaca tcacggaatg aaactcgaag gaagaaaatc
     5761 cctgtaataa caataaatag caggtataca aatatcgcga ttggcatatt cacaagtgca
     5821 agcattacgt tatttaagcc atcgccggta gcaccgccag ttgatggttc actcactcta
     5881 cgaaacaatc ctgtgagacc gctcatccaa acaacaataa ggacagccga aagctttgtc
     5941 atgagaggca ggcgattatt ttcagccttc agagcctgga cagctacata aattgcaata
     6001 aatggcacaa tatacgtagc cgaaccaaat acgctcagga agaaatcacg catccagtta
     6061 agcaattgac cgcccccacc aaaccatgac acaataagca aaagagacaa tgcaattaac
     6121 agaattgccg aaacctggcg ccaaaaaccg ctcggcaact catgctgcgg cgaatcaatc
     6181 ttcttagtct ttcgtcccct ggtggtcttt cgtttttttg ccataacttt tatctattgt
     6241 atgtcaattg acatctgttg tatatccgta tgcttttata gtagcataaa ttacaacaaa
     6301 aatcaatacc gcctatgctt agagagttgc aggttcgcgt ggctcacgag gcacttgcac
     6361 ctctgagtca ggcacctggc gtggtgggaa ctgacggcgc cgcaccgcca tcataccctg
     6421 tcgctgcggt tgactttggt cgccatcatt tgcaatattc gcgttaggtt tagcgccctg
     6481 ctgacggtta ttgttcatgc caatctcgtt aataacagga atcacgatcg gtgtgcgacg
     6541 agtttgatcg tacaaaatgt gcgtgacttc atctttgatc tctttcttaa taacttcaat
     6601 atcgtatttc ccagcaaaag cacgagcagc cttctgctta atgtacgcac gaatcatgcc
     6661 cataagttct tcgctatcgc ggagataaat aaacccgcgg ctaatgatat cagggcttgt
     6721 aaggagccgg ccactaccgc gctgcacagt aagtacaacc acaaacatgc cttcctgtga
     6781 catgtggatg cggtccttaa gcacaacttc gctcacaatt tcgccagcat cgtcgtacat
     6841 aatgccacca acatgaacac ggccaatctt gcgcgcaccc tcatggtcga attcaagaat
     6901 atcgccagca tcacacacaa agatattatc gcgtgggata ccgcattcct tttcggcaag
     6961 ttcagcatta tgcacaagca tatgaaattc accatggtat ggaatgtagt acgttgggtt
     7021 tacagcgcca atcacacgaa tatgatcttc gtagtaaccg tgacctgaaa ggtgaagtgg
     7081 accgatacca gtaagatgcg tcttgccgtt ctggataatc tcgccgcctt cgcgcatcaa
     7141 gccatcaaca gtgcgaacaa cgtacttttc gttacctggg attgggttcg aactaaatac
     7201 aacaacatca cttcctttga ttttgatgta cttatgagca cccgttgcca tacggttcaa
     7261 tactgcagaa aactcaccct gactaccagt acaaacaatt gtaatcttat tgtcaggaag
     7321 tttaataata tcttccatct tcattaccgt gtcttttgga accttaatcg caccagtacg
     7381 aagcgctacc tcaaggttct ggatcatact aaatccagca aaggcaactt tacgtccatg
     7441 cttttgcgcc tcatcaagaa taagctgcat acggtgaagc tgacttgaga agctcgaaat
     7501 aatcacgcgg ctgtttggat atttatccat tacctcaccc atactatgtt gaatatcgaa
     7561 ttcaccgtgc gtgtgtgtac catctgattc acagttggtt gattcattaa ggaataccaa
     7621 aaaaccctct ttttgctgaa tctcagacaa cctcgggaag tcaaacttct taccgtcaac
     7681 tgggttctct tcgatacgcc agtcaccaga tgccaagatg ttacccactg gcgtacggac
     7741 tacaactgct gtcgcatctg ggattgagtg attcacgcgc accagctcga tactaaatga
     7801 atcacaaact tgtacaatat cgtgcgcctc tgggttgagc tcacggtaat ctggagtaaa
     7861 gctaccgtcc gtctcttcca tagtgcgctg caacatacca agggtaaatt tacttgcaaa
     7921 cactggcgca ggaagctttg gcgcaatatg acggaacgca ccgatgtggt cgaggtgacc
     7981 atgcgtgaat acgtgagcgc gaatacgatg cttgttctct tcaagccaag tggtatctgg
     8041 cgtaatgtag ttgatacccg ggtaatcgct acctgggaat aaaaagccca tatcaatcac
     8101 gataatatca ttgtcgtatt cgatagcagt catattttta ccgataccca tctcaccaac
     8161 gccaccgata ggaataatct taagcgttgg ccgtgcagag cgtggctttt taggatacat
     8221 actaaggcta aactgtttgc catcgtagcc attaaagcgc gatttgttca ccggcacgtt
     8281 aataatgtgt tggctcgcct gcatattaac accctcagaa atacgacgtt gtgcacgaaa
     8341 cacctcaccc ttacgagtag tggtagcatt caatactgag ttgtttgact ttggttttag
     8401 ctgactattt ggccgttttg acgaatcatc agcgtttggt tttgcgagac gtcgttgtcc
     8461 catattgttt ttctcctttt ggttagaaaa tacttatcaa taacaaaatt cagaggagga
     8521 agcggcgctg gaccttggcg tacgagtgcg aggagcctac ccttcctatc tataaagtga
     8581 tttctattat acatcgttga aggcattttg gcaagctgtc acacacttaa gcaaggccta
     8641 agtcagggac cgtattgtaa aagcatacag ataacattaa gaggagcagt acatgaatat
     8701 catctcccgc ggaataagaa atgcctttcg caacatcgta cgaagtgggt ctatcattat
     8761 tatcttggcg ctgagtattg ggcttgttat cgcaatgctc gccgcacgcc aagcagtaca
     8821 ggataagatc gcatcagtaa aagctaatac cggcaacaca atcactgtat cgcctgctgg
     8881 tgtacgcgga tttgatggag gtggcaaccc gctcaccagt gatcagcttg caaaagtagc
     8941 aaatattagt catgtaacaa aggtagtcga aacactcaac gaccgactaa catcatccgg
     9001 cacttcctta aaatctggta tcgatccggg cagtttaggt aggcgcaacg ccagcaacag
     9061 cggtatcggc gaaggttccg cagtgttccg cgtagatgac agcaactcag atagcaacgc
     9121 agtacacacc ttctccatac cggtcacgat tacaggcaca aataatgaat cggcgatcaa
     9181 aaccgcaggc acgacgagcc tcagctggaa aagcggcaaa atgttcgaca ctaccaagga
     9241 tgcaaatgta gcggtcattg gtgcgcaaat cgccagcaaa aacaacctaa gcgttagctc
     9301 gacatttaca gcatacaata caacattcac agtcgttggt atttacgacg ccggcaacac
     9361 ctttaataac gacggggtct acattcccct tccaacacta caaaagcttt ctgatcaatc
     9421 tggcgacatc accagtgcaa ccgttacgat tgattctagc gacaacttag caagtgtaac
     9481 aaacacgatc aaaagccagc tcggctcagc ggctgacgta acaaatagtg ctgatattgc
     9541 caccgcaacc accgcaccac ttgaaagtgt tgcaagtatt gctctcttta gcttgattgg
     9601 tgcagttatc gcaggtgcag ttatcattct gcttacgatg attatggtag tacgcgaacg
     9661 acgtcgtgaa atcggagtaa tgaaagccat tggggcttca aacatcgtaa ttatgtggca
     9721 attcatcgtt gaagctatca cccttacggt actagcactg attgttggta cgggtattgg
     9781 cgtcgttgcc gcgaccccac tcacaaacgt gcttatctcc aatgattcaa ccagtagcac
     9841 aaattcagac ccttctatta ctcaagtagg gggtggccga cgaatgatga gatttgcagg
     9901 ccctggccag tttggtcaag ccagccgcaa gacaatcgag aatattcaag cctcagtagg
     9961 tggtgcaacg cttaccgaag gggccgcact tgcgcttgtt atcgcaattc tcggtagcgc
    10021 agcgccgtcg ctcatgatta gcaagattaa gccagcagat gcaatgagga acgaataatg
    10081 ttagtggtta aagacgtaac aaagcaattc aaacagggtg acaacaccgt cacagctctt
    10141 aacaaattat cgtttaccgt gaacgatggt gagttcgtca gtatcattgg caagtctggc
    10201 gccggcaaga gtaccctcct gggagtactc ggcgccctcg atacgccaac aagtggctcc
    10261 attgaaatcg gcgacaccga tattgccaaa ctcagcgacc ataaacttat cggctaccgc
    10321 aacacctcaa taggatttgt gttccaaagc tataatctca tccccaacct cactgcagtc
    10381 gaaaacgtca tgctcccaat ggaatttgcg catatcccgt caaaagagcg tcgtgatcga
    10441 gccatacagc tccttgaaga agttggtatc acagatggtc gcgaagggcg taagccaggc
    10501 aagttatccg gtggcgaaca gcaacgcgtt agcattgcac gcgctctcac taatcatccg
    10561 aagcttatct tagccgacga gccaaccggc aatctcgaca gtgaaaatgg caaaatgata
    10621 ttcgatctat tgcataagct cagtcgcagc gaaaacacca cagtcgttgt tgtaacgcat
    10681 gatcttgata tcgccggcaa aactgaccgc accttcacat tatctgatgg caagctcgtt
    10741 actaaaaaat agtctacaca atgtattaaa aagatataaa aagtatagcc gaggctcgcc
    10801 tcggctatac ttttatcgtc atatcttttt aataatttca ggaatacgac gaaagtcgtc
    10861 aagtaatgtc tcacgcaagc taccattata aagtgctgcc gcagggtgat aaagcggcac
    10921 gatgataatc gtatcatcac caaacttgat gcgctttggc tcgccatgaa tcttgctaat
    10981 atacattcca ggtaagaaat attccatact atgacgcccg agcgtcacga caactttcgg
    11041 tttaataatc tgtagttgct tcagcaaata tggccaaaaa gccttctttt catcaggaag
    11101 cggatcacgg ttattgggcg gacggtattt cacgatattt gtgatataga tatcgctacg
    11161 ctgcatcccg gcagccccaa gcatctcatt caggaacttc cccgccgctc caacaaatgg
    11221 aattccctgt tcatcctcat tcttgcctgg tgcttcacca ataaacacca cctcagcatc
    11281 aaggtttcca tcgcccatca ctagctgtat agcagtttta cgaagttcag gagtaacatc
    11341 agctgcaatc aatcgctccc gcaactcgtc catctgttct tgcttcgtca tactattcgc
    11401 gagaacggat tgccgccaat aaattattaa gctctgtttc tggcagtaaa ctatcctggt
    11461 gtttcttcac gttcttaagg cttgcatccg cattgtattt atcttcaacg tccgtaaagg
    11521 cattgtaagc tttagtgaat gcagtttcat ccttagcaat atacgcaacc tgcagcgcaa
    11581 cgatatctgt acgcatttct gtaaagtact gctctaattt cttgcctatt gttgccacat
    11641 caacattcct tgcagaactc agttctttaa tggctttcgt gcatttagtg aacgccctat
    11701 cccaagaatc ggcaattgtt gaaaggtcac ttgtatccat accagtagta tcggcattat
    11761 tgcattccac caccatagta cgaagtgtcg gcattgcctc agccatacta tcattattca
    11821 ccattgcggc attgtttttc gtgacaaatg catcgtaggc agatttcact ttgtcatctt
    11881 ttagtgcacg catgccagat aagctactga ttgttgtttt ataggcagta ttcgcatcat
    11941 tataggcagc cttcttggtc gcaatgtcac gatctgtcgc agaggtatcc acggctgccg
    12001 tcgaccattc ctctgccaca tcatcggctt tatgatatgc cgcaattgcc gtattggtct
    12061 gtttttcagc actcttatag tctgccttcg acacataaaa gaacgcaaag tacacaacag
    12121 ccaccgcaat tccgagcacc aaaagaccgc caacaatcac aaaaagaaca gtacgcgtct
    12181 tctttgattt gtttggcgac agatttggaa tgggcgattg ttggccggtg atactcggag
    12241 taggcgtaag tccttctgtt gccgatggtc caatcacacc ttctggctta tcttgcattg
    12301 tagacgtcgg cgtttgtggt tgatcgttca cttgcgcgag ctccttacga ttaattctct
    12361 cttaagtgta gcgcattgca tatgtaagcg ctacactcct caaaaaatta tctactgttc
    12421 aatacccttc atagtaaggc taagacggcc tttctcatcg atagcatcaa gtttcacttt
    12481 caccatctga ccctctttca acacatcggt taccttctcg acgcgcttgt cgctgagttg
    12541 gcttatatgg agcataccat cagtacctgg gagaatattc acaaacgccc cgaagtcctt
    12601 aatgctcact actttaccag tgtagatgcg gcctacttct ggctcttcag taagaccacg
    12661 gatccactca agcgcgcgtt caatgctttc accatctgga ctcgctacag tgataagccc
    12721 gtcatcttta atgtcaatca ttgcaccagt ttcagaggta atcttgttaa taacctctcc
    12781 gcccttacca ataaccgcac caatcttatc tggattaatc ttaatcttct caatacgagg
    12841 agcgtacgga ctaagttcag gtcgcggtgt cgcaatcgtt gtaagcatat gctctaagat
    12901 atgtgcacga ccaggcttgc tctgctcaac agctttacga agaacctcaa caggcaaacc
    12961 atgaaccttc atgtccatct gcatcgccgt cacacccttt tcggtcccag taatcttgaa
    13021 gtccatatca cccgcaaagt cctcggcgtc agcgatgtca ctcaaaacgt atggtgtatc
    13081 accgtccatc atgagaccca tcgcaatacc acttacagga cgcttcaaag gtacgccagc
    13141 atccatgagt gcaagacacg agctacaagt agccgccatc gaagtagaac cattctggct
    13201 cataatctct gtcacactac gaatagcgta tgggaattcc tcttcagttg gcaataccgc
    13261 cgtaagtgcg cgctcagcaa ggtagccgtg accaatttcg cgacgacctg ggctgcccat
    13321 gcgaccaact tcaccaactg tgaatggcag tgcgttgtag tggtgcatat aacgacgctc
    13381 gtaatcagtc tgctccatag tgtctactgt ttgcgcaaag ctaagtggag caagcgtcac
    13441 aatattcata ccttgcgtca cgccgcgagt aaagatactc gaaccatgtg cacgaggtaa
    13501 caccccaatt tcacttgaaa gtgggcgaac ttcttcgagc ttacggccat ctggacgagc
    13561 attatcttcg acaatacctt tacgaacatc tttgtgaaga gccatggtaa atgcctcgtc
    13621 gtattggcta cgaataccgg catactcctc ttcacctcca tgctttttag ccatagcaat
    13681 atggaatgcg aggcgaattt cgttcaccat ttcattacgc tctggatatg gcctgcgaat
    13741 cgtagcacca atcttgccct ctacccattc atcggcctct gcctgaatag tagtgtccgg
    13801 cagaacaagg gtatactcct gttcttttgg agcaactttt tctttaagtt ccttctgtag
    13861 tgcaatcgcc ggttggaagg cttcatatgc ccacgccatc gcatcgataa tcacttcttc
    13921 agagacttcc tcggcacctg cttcaaccat tgtgatgccg ctttcgatac cagcaacaac
    13981 aaggtcaaga tcactctgtt cgcgctcttc aggtgtcaag aatgccttaa attcaccgtt
    14041 cacacgacca atgcgcaaac ctgccacagg gccatcaaat ggcgtaccag taagcataag
    14101 tgctgcgctt gccgcaatca ttgcaattgc atctggacgg aagtttggat ccatcgaaag
    14161 taccgagctt actacctgga cttcttgacg gtatccctta gggaatagtg ggcgaatcgg
    14221 gcggtcgatc atacggccaa taaggacagc gtcatcactt ggacgaccct cacgcttaat
    14281 aaagcggctg ccactaatct tgcctgctgc gtaaaacttc tcctggtact caatagagag
    14341 tgggaagtag tcaagcacaa ctggacgtgt tccgacagtg gccgtaccaa gcacaacagt
    14401 gtccccatag gttacaagta cgctaccagt agaacgaaag ccaacgcggt ttacttcaag
    14461 cgtcagtggg cgcccgcata attcagtagt gacagcaaaa atttctttgc cactagggtt
    14521 aatgatactc atgaactgag cctcctcttt gttaggtcgc tcaccttcta ttgtgtaagt
    14581 aacgggtaga aaatttctgt tgaattcttc tatccaccac ttactgtggc aagcgtgtgt
    14641 ttattatacg ctattttccc tctcattacc atcactaccc ctgttactaa aagcttttag
    14701 acaaccaaaa tttaaccaca ataatagccg gcatattgta ccaaaacatg atattgcaat
    14761 gcttgtctat tgctatgatc gttcggtaaa aatgtattca ataacttcct cgccacgctg
    14821 gcaaagggta tcgaaaccat caagttgttg atcgagattc ccacctgttg aatcttgtga
    14881 attgatcatt acgataatgt attataacat cttacataat aaacagcaat acttcgcgca
    14941 atgtaacaga attgactaag ccacaatatc tctcatacgt tcacgtaacc gttcttgacg
    15001 tcggtctggc gtaatatctt catgtatctg tgagcgcgac aatatacctg catcagtcat
    15061 tccccgcaca tggagcgagc agttcagatc ggcacataca agatacgaaa ccgtacgatt
    15121 atcacctttg ggcaatgtaa gcacagcact ctctggccct cgccgccacg ttgcacaaat
    15181 atcacagatg attgccgcaa ctcgtccgcc cgttcgtgct acccgtggtg ataatgtaaa
    15241 cggtatagtg gtatcttggt ataacagcac gccttcattg ccagatttat cggtcacagc
    15301 caaaaaatct cgagagtccc attcagtaac ctcttctggc acgaagcgaa gggtcttccg
    15361 tagtcgtggc ttaatacgtg cagatgcaac acgttccaca aattcctcac gcgttatttt
    15421 atgcatcttc tcgttcctta agccaaagag caaacttttt gtacgcgcct ttagcgctgc
    15481 gcttgtcgtt catctgatga tgttcctcag gacgtgactg cgaaagtgtt accgagcgtc
    15541 cttcagggat aaatactctc cataaaggtc cccatgcttc cgggcgttca acttcatcca
    15601 ccgcatcagc aatcataccc caatatggtt catcactaaa tgcgtactgt tcaccgttct
    15661 catcaacaaa tacaagatta ctaataaaat acgctcggcg attatcatag ccctgcatca
    15721 atttcacgag cccctctgcg ccaatcttct tacccatgta tttttgatac ggccctggga
    15781 atccgcccag cgcctcaata caaaactcac tgtcatctac cacaactggg tgacctagtt
    15841 gcttaaaggc ttcaacggcc ttcactttag caatctctag tgcggtgtct gcctgtatct
    15901 cagtaatttc aagctgcttt gcaatgacga taatatcgca gcccgcccga tcgaggtgag
    15961 cctgcaatga cacaactttt ccattgttgg ttgtcgcaaa aatgagttct ttcatacata
    16021 cccctaataa aagaccccgc cgatgagcag ggtcttttac gctagctact tacgtaggcc
    16081 tagcttttca agcgttgcct tgtaggcctc aaagtctttc gtctcaaggt acttgaggag
    16141 gcgcttgcgc ctacctacca tctgaataag accgcggcgc gccatgtggt cgtgcttatt
    16201 ggccttaaga tgctcagtaa cctctttgat acgagccgtc aaaaccgaca cctgagcctg
    16261 tgggctacca acgtcgtttt tgttaacctg agtcaaagca atagctttcg ctttatcatc
    16321 ctttgatatc ataactggta attgtaacag ataagctact aattttcaac accgaagtca
    16381 gaaagcgcct ttgcatcagt taccgagaaa tcagcaatct tcgtgcggcg cagctcctta
    16441 cagtacgcgc ccgtgcctaa tctcattcca atatcaaccg ccaagctacg aatataggtg
    16501 ccactactca catgggtacg aattgtaagt gatgggtatg aataatctat gagctcgaga
    16561 ttgtacacgg tgattgttcg ctccgggatt tccacttctt taccatcacg cgccaacttg
    16621 tacgctcgct ggccattaat cttgatggcg ctaaaaatcg gtggacgctg cataatttca
    16681 ccgcgaaacg ccgcaagcgc cccctcgact tgttctttcg tcggcacact atctgaaacc
    16741 tgcgttaact caccctcagg atcgcccgtt gtactctctt ggcctaatat aaactctgcc
    16801 tcatatactt tatcaagctt agtaaattcc atcgccttac gtgtcatttt gccactcacc
    16861 aaaatcaaga gccctgttgc aaatggatca agagtgccgc aatgccctac cttcactttc
    16921 ttacctgccc ggcggctcag aatacggcgc acgcgcgcaa caactccaaa actggtcatt
    16981 ccggctggtt tatcaattaa aataatttct ccgtccatca tcgttattat agcgcactcg
    17041 ttttcggatc gatgttacac ttgaaatatg aatgataaaa atgtctggac agcgctacat
    17101 gatgattaca agcaacggga ttgggcgaaa attccatcaa tgtttgccga aacatccata
    17161 gcatatttcc cccagcaagg tcgagtactc gaccttggcg caggacttgg ccaagatagt
    17221 cgtttcttcg ccgaaaaagg atatgacgtt gtaagtacag acctcgaaaa tggcacgctt
    17281 gaagaacagt ttgccgatct accatccgag ttaaaaaata agctcagtac ggcacgagtt
    17341 gacctccgcg aagaacttcc atttggaaat ggctcatttg atgttgtata cgctcaccta
    17401 tcgctccatt acttcgacta tgaaacaact gtgcggctta tcggtgaaat tcagcgtgtc
    17461 ctgcgacctg gaggcgtcat tgcctttttc gttaactcaa cgagcgatcc agagtacgca
    17521 tctggaacaa agatcgaaaa cgacttcttc caagtgggcg acgcaacaaa acgatatttc
    17581 agtattgaga cgactcgaga attcactaaa tatatcgaca cgactcttct tgatgacaaa
    17641 ggcgaaacct ataaagacgc cgctaaaggt gtccacaatc tcattcgttt cataggaacc
    17701 agacgccaga aagaagcatt ttcaatggcg ctcccttact gtggcgcaat cgtcgaacgc
    17761 gaacaaaacg gcgaaaaaga gcttctcatg caaactcgct ggaaaccaca cgccgaccct
    17821 gtttactctg gaacaattga gtttccggct agcacccttg ataagccatt cgaagatatc
    17881 cacgataccc ttgcccggga aatcaaagag gaaacaggtc ttacacttaa gaatattcga
    17941 ggcgatgaac gtactagtga tataacaagt ggcagagatg atcaaatcat cggttttcga
    18001 ccgttctgct gtacgcagca aatcaaaaac ggcaaaccat ggataggatt cgtgttcgta
    18061 tgtgaagtcg aggctggtga gccaaaagct caacatggtg aagcaaaaga cgcccactgg
    18121 atgaaggctg gtgacttacg ccaactatac gagaatgaac ctgaaaaact cttcggactt
    18181 gagttacccg catggcaata ctattttgat tcggagaaat aacaatgaaa aaagtattat
    18241 ttggcatttt tgcccacccg gacgatgaag catttggccc tggcggcaca ctcataaagg
    18301 ccgtacacga tggtgtggaa ttacatctta ttacacttac cctcggccaa aatggcacca
    18361 accctgacaa cgctcctgat ttaggtaccg tgcgccgtga ggaatggctg aaaagcggcc
    18421 aaatgatagg cgctacaagt caacatgagc tcggctatat tgacggccgc ctctgcaaca
    18481 ttgagatgct cgagattact gaaaaaatcc agcatatcgt gcgcgaaaca attgcagatc
    18541 gtgacataga agttgaattc ataggatttg atctcaatgg tcttaccggt cacattgacc
    18601 atattgttgc aagccgagca ctctgcctcg cattttaccg tctcaaacaa gacgatagcc
    18661 gttttactcg aatccgtcta ttttgtctac ctcgagagca aaaccaaagc gtcaacacca
    18721 actggattta catggaagcc ggccgcacgc cagaagagat agacgagacc atcgatgctc
    18781 gcaaatacta caaaaaaatt atagatgtaa tgcgtacaca ccatagccaa cgtagcgacg
    18841 ccgaaaccca aatagacacc cttggacacg gtattgggat aaatcatttt ataatcctta
    18901 gctagctcta ctgaccagga attttgaact gtgcgggatc tatagactgc ggcggttgct
    18961 ctgctggtgc gggcggcaaa actgcgccaa gcgtatcagg aacagaagct acatttacag
    19021 gggctgggtc tccaggaagc ggtggtagcg ttgaaaaatc tggcattggc ggtaatgggg
    19081 gcagtggagg gagtgccgga gctacctctg ccgtcggtgc tgggattggt gcaggttcat
    19141 atgctggcat ctcaggagcc ggctgttcct gcgtattaag cgcctcatca agtgcagcgc
    19201 gtgcatcatt atgcgcggca cgattttgcg catcaatttc agcaagtgtt ggctcagaag
    19261 aaacaaaagg caccggaggc tggatgatgt cagcgtggcc agttggagca gtaccgctct
    19321 cctccgcaag cacgtcgcga acttctggct cagcatctat ctggcccgca gccgcattaa
    19381 tcggtgactg gaaagcaggc acaccagttg catacgagtt attatggctt agaatcgtat
    19441 gattgcgatc gtcttccagc tgttgacgct tgtcttcttc cgcctgttca gttgtcgcat
    19501 tcagcgtccc accaaaactc ggaacacctg tctgttcatc ggtatgcgcc gttaacgacg
    19561 actcttgagc gacaggagcc actgttgaag gggctaccgc tggaagctca aacacaggtg
    19621 gagctgctga cacggtcgtg gcaagctgct ctgcaagctt ttgctcggct gcctcagtcg
    19681 cagcttcttg acgagtctcg ttcacgcgtt ctgcctgctt tgcaagttct tgctcggcgc
    19741 gacttatcgt tgggctggaa ttgcctgtag cgatctctct cgctacagta tcaacgtcac
    19801 cttctggtgc atgcgaaatc gagagtgcac caatagcacc attatcatct ttctgcactt
    19861 ctttttgcag gtcggtaggt ttctccccta ttgaagtatg cgcaacattt gcgtgagtcg
    19921 aaattgccat atcttcttca agttttgtag caataagctg ctggttagcc ccagctgaca
    19981 tcaactgcgc ggcaacagtc atcacctttg agcttgtctt gttattcgag aatctgtcag
    20041 tcgccgcaac gataccagtc agaaacgccg tagcaacctg ctcatcaagt aaagttttct
    20101 cccctttcac accttccgcg atctgaaata gcatttcgct atagctactc gcctcactat
    20161 cagcccactc aataccacca agttttgttg gctcgccaag tgacactgcg gcaacggttg
    20221 cgtcatgtaa gatacgacca tggctttcaa gtgcacgatc aaggtcagtt tcgttctgta
    20281 caccaacagc taacacgaat tctacgttgt aatcaccctg agaaaattca aggtctttct
    20341 cgctaattgt tgttcggtac ggtgtgataa atatcttcac catgtcatcc accactttat
    20401 agcgaagatg gtcagctttc tctttatcga gggcaatgat gaagtctcgc agactatcaa
    20461 ccgtattctc aaacgttttc tcagggttca aaaactcgat cgcaggtggc acattaccgc
    20521 taaacacagc tgtggcatgc ttattcaatt tattaataca aagcgttata gcaagcgctg
    20581 cagaaagctc gtcaacagac gggttcgagt taacagtaac aagaacgttg ttgctatctt
    20641 tgagcttttg aataatttgc tttagcgcgc cttgatctgc cattatacgt acctgctgtt
    20701 tatgttcttt ttcgagtttg gcttcactat accataagta gttcgcctcc gtcaatgatt
    20761 attcatatcc tctttacaaa caggggtatc tcaggtataa tcagtctttg attgaaatag
    20821 tagctctaaa gataagatat aaggaacaat atgccaatca tcaaatcagc aatcaaacga
    20881 atgaagcaga ctgcaaagcg tcgcgcacgt aacgtaagca tcaagcgcga catcaagggt
    20941 gcagttaaag cttttaccgc tgaaccaacc gccgctaacc ttagtgccgc acagagcgaa
    21001 cttgacacag cagttaagaa aggccttatc aagaaggcta cagcaagtcg ccgcaaggct
    21061 ggtcttgcaa aagctgccaa agctgttggc gctaagcctg tagcagctcc aaaggcaaaa
    21121 actgctgcaa aatctgcggc aaagaaagct cccgcaaaga agcctgcaac gaagactacg
    21181 acaacaaaaa agaccgcggc aaaggctacc acaaaaaaag cctaagctag gttatagtaa
    21241 aataatctcc tagactaggg gattattttt tatcgcgagg taatcgcata caaaaaacga
    21301 tagatcgcat cccacggccc cgcctgagac gtcttagtct cgatatcaag gtcggcaata
    21361 aactgcgtca atgtacgaac ctcactactt cgaaaacgac tagctgtctc ctgcattttc
    21421 tttgctacga acgggtgtag cccaagttct gttggaatac tttgcgacga gccaccaagt
    21481 gttgcaacgg ctgcaagttg tgcccattgg ctgttcaata gtgctaatgc tctatggccg
    21541 tcatcagtgc gtgccatgtc agttagtaac gtgtcgaggc gcgccaactc acggcgtgca
    21601 gcgatatcaa gcatatcaaa cacgctgtcc accattgtct gcggcaaaat cgtagcaatc
    21661 atatcattgg tcacttcttt agtccccgca agagccctca ccgcatgcat gagttgcatc
    21721 tggtcaacca cgcgcgcaga taccttatca ctcacaacca gagcccgttc gaccatttgc
    21781 gtaacttgcg ttagtgatag tgacacatta tactgcttcg ccgccgttcg caaccactcc
    21841 tcgacggccg ccgtatcgcg ctctgtaaat gaatctgcag tgaccactgt tgctgttcta
    21901 ataatcgcct tatatgcctt agtgcgctta tcgagcttgc gttccagcaa tactatcgtc
    21961 gtttcatctg gcacctgctc agcccactca gcaagcttgc cgaataatgt catatccctg
    22021 gagagctcac gaatcactat aatgcggctc acagcaaata aagacccacc gcgcacaatg
    22081 tcagccaaag cattcagcga aagctcgtct gtgtcaatat gctctggcgc tacgccgacc
    22141 gctctcgcaa tcttctgcaa ctccactttc tgcaggtagg cattctcacc gtgaatcaga
    22201 tatatcatta catctatgat actacgaaac agaggtgacg actacagtgg cgtaggttgg
    22261 caaagagggc aaatatgggt accacgacca gcaactttca ttttaatgat aatcgtaccc
    22321 ggatgacgtg gacattcttt accttcacgg cgaaaaacac gggcaaaatc gatataactt
    22381 ccccgtttgc cttcggcgtt tacataattc ttatcggttg aaccaccctt ttcaatagca
    22441 agattgagta catatttaag ctcacttagt aatatcacaa gctgttcatc gctcacatcc
    22501 tttactcgcg tcgccgggtg aattttagca ccccataggc tttcatcagc gtaaatatta
    22561 cctactcctg ccagtaccgt ctgatcaaga agagcagcct taatcgttgt attattacgc
    22621 cgccgaagtc gtaatataaa catgtcagcc gtgaaactat cttcaagcgg ctctggccca
    22681 accttctgca taaaatcaat gttcatcact tcgggtgtcg ggtagagctt catccaacca
    22741 aacttacgct ggtcattaaa aaacagcatt gtaccatcat gaaacgcgat caccacgcgt
    22801 gttgatcgat ctggtagatt accgaccagc gagtcagttg gatgccctgc cccgaagcgc
    22861 tcgctcccca catacactaa ttgccccgtc atctttaaat gagtaacaaa tgtgtaattc
    22921 gtagaaagct caatgagcaa cacttttgct cgacgtcgca ctgccacaat cgttgcgcca
    22981 tacgcaaatt gttcaacatc gtcccagtta ttcggaaaac tctttggtga atcatgaacc
    23041 attatagctt taacacgtcg acctacgaca agtcgctcaa gaccgcgacg tactgtttca
    23101 acttcaggaa gttcaggcat atactaacta gcttgggcaa caatctcgtc gattctttgc
    23161 ttgatacctg catttgtttc aaaaacaaac gcgtgcatcc caacatcctt tgccccagtt
    23221 acaaaacctt cgcggtcatc aataaagata cattcctcag gcaatagccc aagtcgagct
    23281 gcagctgtct catacattgc agcgtgcggt ttatcaacgc caatctgaaa cgagaatacc
    23341 ttctcgtcaa agagcgccac ctggtcggga cggaatagct cgttcgtcca atcatcagat
    23401 gcgttactca gtaagccgat cttataattt ggctttagtg catcacgaat ataatcaaat
    23461 agttcattgt ttggcgagcg ttgttcaagc gtggttttca catcattcac atcaatatgc
    23521 gacaagtcgg caatagactg catgaattca tcatgggtaa tatatccaac gtcggcctgg
    23581 tgattaagtt gagatatctc acgatattca cgagttccct ctttaaagtg ttccccacgg
    23641 aactgcaacc acccatcggt tgtaagtacc ccaaaacagt caaagataat accgcgtatc
    23701 atacgctcat tgtaccactg ttattaataa atcttggtaa acagcggacg gaagtcagga
    23761 acttgattca cgaaaagcgc ctgcacctgc aatgggtccg cggtcattgt accttgtgaa
    23821 tctttacaag ttgagctcca catcgtgtga tctgggtcgt cattaaccaa tgtttcaaat
    23881 tcataggcga gtccgttggt tgcacaaacg ccacgcagat ccgcatcttt tgtatcacgt
    23941 gctttagcaa tatctgtctt atcaagcgca tatacgaact gttcatatgc ttttaaatta
    24001 ttgccgtagg tcttcgtatc aattacttgg tcaaggtagc ctgaatacgt tacaaacgta
    24061 cgagttgaag ggctaatgat aatttgatag ctgcggaatt tttcatctgc tacaattggg
    24121 ccgcgaactg tccaacgtac agaacgatca ctatcctgtg cgagaactgc ttgctcaata
    24181 acggatgagg tagtcttttt attatcccgt gtgtcaccac tgccgctaaa caacatgcga
    24241 cctactgtta cgatagccgc aacaacaagt gcgacaatca caatcacgac aatgattgga
    24301 agaattcgtg gtcgagatcc tctatacata agaggactat atcatatacg ataatatgcg
    24361 tcaatgtgaa gctatgagcg cttttgtcat ttgatatgaa cctcgctctt ctcggcttgt
    24421 acctgatcta ttgtaataat ttaccatact gac
//
LOCUS       DATIIX010000033         5986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_397101, whole genome shotgun sequence.
ACCESSION   DATIIX010000033 DATIIX010000000
VERSION     DATIIX010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397101"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..719
                     /gene="cyaB"
                     /locus_tag="VLF64_01025"
     CDS             156..719
                     /gene="cyaB"
                     /locus_tag="VLF64_01025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HSW90558.1"
                     /translation="MREIEIKVKLANLDEAKQRLVAAGVAISQPKRHHDVVYCLPENL
                     QSVGGDTSVNWLRVRTENDSTVYFTLKRSVSGSLDSIEHETIVEKGDELVKILEYMGY
                     VVFSELAKIRQTGHYSESIEVCVDEVPPLGGFIELEKLCPDDADGSAVEKELFTALDT
                     LGIEYTERMTNGYDELMNDHLAQSEEK"
     gene            736..1221
                     /locus_tag="VLF64_01030"
     CDS             736..1221
                     /locus_tag="VLF64_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90559.1"
                     /translation="MGWFTDAVFGNGDNTNSGSDNKRQTGADDPYRQASGGKVIPEVV
                     VDNADAHCSADMSHMELWVRLKNTSTFEVEVVEVRILGQSQGLNRFLKPGENHDCCVY
                     KGSVPTNNAQHVAHVDYKIVGNGDYFESDHQINYRLEDDNGVHHFIPEELKLLRPVRD
                     L"
     gene            1277..1699
                     /locus_tag="VLF64_01035"
     CDS             1277..1699
                     /locus_tag="VLF64_01035"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HSW90560.1"
                     /translation="MTRVFYTDGSASPNPGPGGFAVVEGKGPVALGSESGDTTNIRME
                     GHAIIAALKLADGAPCIIHTDSEFWINVITKWAPGWAAKGWRKSGGPIKNLELVQEAY
                     ELYNTSHATLSWVRGHVGHEGNELADEWANKAREGARV"
     regulatory      1812..1912
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1963..2952
                     /locus_tag="VLF64_01040"
     CDS             1963..2952
                     /locus_tag="VLF64_01040"
                     /inference="COORDINATES: protein motif:HMM:NF014792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HSW90561.1"
                     /translation="MSAIAHHYRTAESVSPSHPDKMCDQISDAILDAYLVQDPDARVA
                     IEACGGHGTVFLTGEVTSHAEVDAIAIAKRIAGNMKVIEHIAKQSQEIAGGVDTGGAG
                     DQGIMVGYACNETPQLLPLEVVLSRELNQYLYRIWPYDGKTQVTLRDSVIESVVASFQ
                     NAPHDKLEAMVTEWLRDIHERYNVLRADDVRLHVNPAGDWHQGGFDADAGLTGRKLVV
                     DNYGPRIPIGGGCFSGKDGTKVDRSAAYIARRIAVDYLRKRDAREVYCYLAYAIGYDQ
                     PLEAIAVIDGKEELIQGYDLSPRGIIAALNLTEPQFEQTAQYGHFGNGFAWDR"
     gene            2993..4516
                     /locus_tag="VLF64_01045"
     CDS             2993..4516
                     /locus_tag="VLF64_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIF1 family DEAD/DEAH box helicase"
                     /protein_id="HSW90562.1"
                     /translation="MDQGLAIEILLSGESALLTGPAGTGKTFVLNQFIALAKYEGKHV
                     SITATTGLAATHLSGTTIHAWSGMGVRDDIPDGFADHISKSRREIIEKTDVLIIDEIS
                     MLHDFRLDMVDEICRLVRRKDEPFGGIQVVMSGDFYQLPPVNRGDSCGGGFVVNSNVW
                     KEMDPVILYLTNQYRQNEGDKLLEILTSMRENDIRRHHAEALLNRVDKPLPGGIELTE
                     LHTTNIDVDRVNAGHLARLEGDELIYQQHTTGSANYVENLQRSVLAPAVLTLKQGALV
                     MTVKNDPSRRFANGSIGKVVDFEPGTEYPVVEFLNGKVVTIMPDTWELRDGDKKRASI
                     HQIPLRLAWAITVHKSQGMTLDAARIDLRKAFVPGMGYVALSRVKSLDSLYLSGINRM
                     ALQMSEDAHLIDDMLRNRAQSDKQRFAHLTEKAKEREANPPKKKKTDSGWAEKIAKMR
                     ETYPNAYRPWTDDDDMVLKQEFQNGASVKDLSNKLGRHEGSITMRLQKHFGEDIVQL"
     gene            4529..5536
                     /locus_tag="VLF64_01050"
     CDS             4529..5536
                     /locus_tag="VLF64_01050"
                     /inference="COORDINATES: protein motif:HMM:NF027488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kiwa anti-phage protein KwaB-like
                     domain-containing protein"
                     /protein_id="HSW90563.1"
                     /translation="MTTEPTDNTTETRESTDIFLWANQADAHKDALEVDLFMFTKGYT
                     VYATNYAKELKAQLKVLFLYDMISQVQTGAATGMTVRDFEAAAAEDNVLERTTLDKVE
                     HAQEVIEQIMYGEESLEVFREGDHEFKKVKGIIARFSRPNAEPFFVAKLLPQSQVLKG
                     ATAWMYNGDSFQPFSADAGLRITPDNQALVAGGDVFAFSETKFIRMFGYDAKKFAVAE
                     EKIKEIEEHFKLKFPEGMTFDSLVRDTPSLVSKLQKVDPTIVTQDQVVDHADEMDLEL
                     LTDENTGEIIIMDAKDAAKFVNLLNDDYVTSDMTGIKYELKGKKELRPNESASDAPSG
                     M"
     gene            5648..>5986
                     /locus_tag="VLF64_01055"
     CDS             5648..>5986
                     /locus_tag="VLF64_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HSW90564.1"
                     /translation="MLNRQETGFPKKFLWGVSSNAHQVEGGQSNQWTVWELENAKALA
                     AQAPYHYDDLDSWEGYEALATQPPNYVSGVAVDHYELYERDLDLVRKMNMNAYRLSIE
                     WSRIQPQKDAW"
BASE COUNT         1672 a         1138 c         1681 g         1495 t
ORIGIN      
        1 aacactagtc tagtgtgggg tggtattgta cccacaattt tccacctaag cagtaacgct
       61 atatatgggc acgctatatg tagcgtgtac ataccagaac ggattcgcta agatgctcca
      121 tgctaaaata gagtaatgat aaagggaacg aagtaatgcg ggaaattgag ataaaggtaa
      181 aacttgcaaa tcttgatgag gcaaaacaaa ggctcgtggc agcaggagtt gcgattagtc
      241 agcctaaaag gcatcatgac gtcgtatatt gtttacctga aaacttgcaa agcgttggtg
      301 gtgacacctc tgtaaactgg cttcgcgtgc gcaccgaaaa cgatagcaca gtttacttta
      361 cgctgaagcg ctcggtgtct ggtagccttg atagtattga acacgagacg attgtagaaa
      421 aaggcgatga gctcgtgaag attcttgagt atatggggta cgtggtgttt agtgagctag
      481 ccaaaattcg ccagacgggc cattatagtg aatcgattga ggtttgcgtc gatgaagttc
      541 caccacttgg tgggtttatt gaacttgaga agttatgccc cgatgatgca gacggttccg
      601 ccgttgaaaa ggaattattt accgcccttg atacgctggg aattgaatat acagagcgta
      661 tgactaacgg ctatgatgag ctaatgaatg accacttggc acagagcgaa gaaaaatagg
      721 caaaggaggc agactatggg atggtttact gatgcagtat ttggaaacgg cgacaataca
      781 aatagtggta gcgacaacaa gaggcaaacg ggagcggatg acccatatcg gcaagcatcc
      841 ggaggaaaag tgattcctga ggtggtagta gacaatgctg atgcgcattg ttcggctgat
      901 atgtcgcata tggaactatg ggtacgcctc aaaaatacct caacatttga agttgaggtg
      961 gtggaagtgc gaatactcgg gcaatcgcaa ggactgaatc gctttttgaa accgggtgag
     1021 aaccacgact gctgtgtgta taaggggtcg gtacccacta ataatgcaca gcatgtagcg
     1081 cacgttgact acaaaattgt tggcaatggt gattatttcg aatctgatca tcagataaat
     1141 tatcgtcttg aagatgataa tggcgtgcac cattttatac ctgaagagct gaagttactt
     1201 cgaccagttc gtgatcttta gttctcgtag tgcggacttt gcattttcgc aagcacccgt
     1261 gaatattgta gtaactatga cgcgcgtgtt ttataccgat gggagtgcta gtcctaatcc
     1321 agggccgggt ggttttgccg tggttgaagg caaaggtcct gtggcgcttg ggagtgaatc
     1381 gggcgatact acgaatattc gaatggaagg acatgcgatt atcgcagcgc ttaaactcgc
     1441 cgatggcgcg ccttgcatta ttcatactga tagtgaattt tggattaatg tcattacaaa
     1501 atgggcgcca ggatgggcgg ctaagggttg gcgcaaaagt ggcgggccaa tcaagaacct
     1561 tgaattggta caggaagcat atgaactata taacacttcg cacgcaacct taagctgggt
     1621 gcgcggccat gtagggcatg agggcaacga actcgcggac gagtgggcaa acaaagcacg
     1681 ggagggcgcg cgagtataac ctgctatgag tgctcgtcgc tgggttatca attggcctag
     1741 ctcagctcat gggaattgag atctttggta gatattgtgc aatactatgg tacaatagag
     1801 ttattaacaa ccaagtatca agagtgtagc gagggactgg cccgttgacc taccggcaac
     1861 ctgtgatttc ataaggtgct aaatccagcc catttgccgg gcgagatgct attttcttat
     1921 aaggaaactc ctttcgcctg tatgtggatg aaaggagttt tcatgtctgc aatagcccat
     1981 cattatcgaa ccgctgagag tgtcagtcca agccaccctg acaaaatgtg tgatcaaatt
     2041 agcgacgcta ttctggatgc atatttagta caggatcctg atgcgcgtgt tgctatagaa
     2101 gcgtgcggtg ggcatgggac ggtattttta accggtgagg tgacctcgca tgctgaagtg
     2161 gatgcgattg cgattgctaa acgtattgcc gggaacatga aggtaatcga gcatattgcc
     2221 aagcaaagcc aggaaattgc aggaggcgta gatacaggtg gcgctggtga tcaaggaatt
     2281 atggtgggct atgcgtgtaa tgaaacacca caacttttac cacttgaagt cgtgttgtcg
     2341 cgagaactga atcaatattt gtataggatt tggccatatg atggcaagac gcaggttacg
     2401 cttcgcgata gtgtgattga gtcggtagtg gcaagctttc agaacgcacc gcacgacaag
     2461 ttagaggcta tggtaacgga gtggctgcgg gacattcacg aacgctataa cgtgttacgg
     2521 gcagatgacg tgcgattgca tgtcaatccc gccggtgact ggcatcaagg ggggtttgat
     2581 gcagatgcag ggctgacggg tcgtaagctg gtggttgata attatggccc acgtattccg
     2641 attggaggcg ggtgctttag cggtaaggat ggcacgaagg ttgatcggtc ggcggcatat
     2701 attgcacgcc ggatagccgt tgattatttg cggaagcgag atgctagaga ggtgtattgt
     2761 tacttggcat atgctattgg ctacgaccaa ccgcttgagg cgattgcggt tattgacggc
     2821 aaggaggaac tcattcaggg gtatgacttg agtccgcgcg ggattatcgc tgcacttaat
     2881 ttaaccgagc cacaatttga gcagacggcg cagtatggtc attttgggaa tgggtttgcc
     2941 tgggataggt agcgagcggg ctattagggt cgccgtggta taattaaggt aaatggatca
     3001 ggggttagca attgagattt tgctttcggg agagagcgcc ttactgacgg ggcctgcggg
     3061 aaccggcaag acgttcgtgt tgaatcaatt tattgctcta gcaaaatacg agggtaaaca
     3121 tgtgagcatt acggcaacaa ccgggcttgc ggcgacacac ttgagtggaa cgacgattca
     3181 tgcgtggtct ggcatggggg tgcgtgatga tattcctgat ggatttgcag atcatatatc
     3241 gaaaagccgc cgcgagatta ttgaaaagac agatgtactg atcattgatg agattagtat
     3301 gctgcatgat tttcgtttgg atatggtgga cgaaatctgt cgactggtac ggcgcaaaga
     3361 tgagccattt ggtggcattc aggtagtaat gagtggtgat ttttatcagt tgccacctgt
     3421 gaatcggggc gatagctgtg gcggtggatt tgtcgtgaat agcaatgttt ggaaagagat
     3481 ggatccggtt attttgtatc ttactaatca gtatcgccag aacgagggcg acaaattact
     3541 ggaaatctta acgagtatgc gtgaaaatga catccgtcgt caccatgcag aagcgctact
     3601 gaaccgcgtt gataaaccac tacctggtgg gattgaattg actgagcttc acacaacaaa
     3661 tattgatgtt gatcgggtga atgcggggca tttggcgcgc cttgaaggtg atgaattgat
     3721 atatcagcaa catacgacgg gcagtgcaaa ctatgttgaa aacctgcaac ggagtgtgct
     3781 tgcaccggcg gtgcttacac tcaaacaagg ggcacttgtg atgacggtga agaatgaccc
     3841 atcacgtcgc tttgcaaacg gaagtattgg caaggtggta gattttgagc ccggtacgga
     3901 gtatccggtt gttgagtttt tgaatggcaa ggttgtaacg attatgccag atacgtggga
     3961 gcttcgtgac ggcgataaaa agcgagcgag cattcatcag ataccgctgc gccttgcatg
     4021 ggcgattacg gtccacaaaa gccaaggtat gacacttgat gcggcgcgga ttgatctgcg
     4081 caaagcattt gttcctggta tgggatatgt agcgctgagc cgagtaaaaa gtttagatag
     4141 cctgtattta tcaggcatta atcgtatggc cttgcagatg agtgaagatg cacacttgat
     4201 tgatgatatg ttgcgtaacc gggcgcagtc ggataaacaa cggttcgcac atttaaccga
     4261 aaaggcgaaa gaacgcgagg caaatccgcc aaaaaagaaa aagacagata gcggctgggc
     4321 agagaaaatc gcgaagatgc gtgagacgta tccgaacgcg tatcgcccgt ggactgacga
     4381 cgacgatatg gtgttgaagc aggaatttca gaatggtgct tcggtaaaag atctgagtaa
     4441 taagctagga cggcacgagg gaagcatcac gatgcgattg cagaagcatt ttggtgagga
     4501 catcgtgcaa ttgtagtaaa ctagagaaat gacgacagaa cctaccgaca acactactga
     4561 aacacgagaa tcaactgata tttttttgtg ggccaaccag gcggatgcgc acaaagatgc
     4621 actggaagtt gatctcttta tgtttacaaa aggctatact gtctacgcca caaattacgc
     4681 aaaagaattg aaggcacaac tgaaagtatt gtttctttat gacatgatta gtcaggtgca
     4741 gactggtgcc gcaacgggca tgacagtccg tgattttgaa gctgcagcag ccgaagataa
     4801 cgtgctagaa cgtaccacac ttgataaggt tgaacatgcg caagaagtaa ttgaacaaat
     4861 tatgtatggc gaagaaagcc ttgaggtgtt ccgcgaaggt gaccatgaat ttaaaaaagt
     4921 gaagggtatt attgcgcgct tttcacgtcc gaatgctgag ccattttttg ttgctaagtt
     4981 gcttccacag agccaggtgc ttaaaggtgc tactgcatgg atgtacaatg gtgactcatt
     5041 ccaaccattt agcgctgatg ctggtcttcg tattacaccg gataatcaag cgcttgttgc
     5101 gggcggtgac gtgttcgcat ttagtgaaac aaagtttatc cgtatgtttg gttatgatgc
     5161 aaagaagttt gccgtagccg aagagaagat taaagagatt gaagagcatt tcaagctgaa
     5221 gttcccagaa ggtatgacgt ttgattcgct cgtacgtgac acgccaagcc tggtatcgaa
     5281 actgcagaag gttgacccta ctattgtgac gcaagatcag gtagttgacc acgccgatga
     5341 gatggacttg gagcttttga ccgatgaaaa tacaggcgag attatcatta tggacgccaa
     5401 agatgcagct aagtttgtga acttactcaa cgatgactat gtaaccagtg atatgactgg
     5461 tataaagtat gagcttaagg gcaagaagga gcttcgtcca aacgaaagcg catcggacgc
     5521 tccgagtgga atgtagccga tattacaacg ccgcgtaaag ggtcgcattg ttgcgactct
     5581 ttatgtttac caagctagat tactgcaaaa aataggtcgc gaacagctgg gtggtatact
     5641 agtatatatg ctgaatcgac aggaaacagg atttccaaag aaatttttat ggggcgtaag
     5701 ctcaaatgct caccaggtag aaggtggaca gtctaatcaa tggacggtat gggaacttga
     5761 aaatgctaag gcgcttgcag cacaggcgcc ctatcattac gatgatcttg atagctggga
     5821 aggctacgaa gcgctcgcca cacaaccgcc aaattacgtt tcaggcgtgg cagtagatca
     5881 ctatgaactg tatgagcgtg atctagacct cgttcgcaag atgaatatga acgcgtaccg
     5941 tttgagtatc gaatggtccc gtattcaacc gcagaaagat gcttgg
//
LOCUS       DATIIX010000034         5947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_119976, whole genome shotgun sequence.
ACCESSION   DATIIX010000034 DATIIX010000000
VERSION     DATIIX010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119976"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..818)
                     /locus_tag="VLF64_01060"
     CDS             complement(57..818)
                     /locus_tag="VLF64_01060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HSW90565.1"
                     /translation="MSDVKLDDIVSLAKRRGFIYQGSDVYGGLSGTWDYGPLGVALKR
                     NIMQLWWKMFVEARDDMYGVDAAILMNQKVWQASGHVDTFTDPLVECSNCHARHRADK
                     LEDPNKCPTCGMSGTFGEPKQFNMMFKTNVGPVADESSISYLRPETAQGIFTNYKNVV
                     DSFYPDLPFGIAQQGKAFRNEISPRDFIFRSREFEQMEIEYFVDPEHWQEAFEQWVAQ
                     CKEWFAALGLPAEDIHELEVPEDDRAHYSKRTIDF"
     gene            complement(982..1656)
                     /gene="recO"
                     /locus_tag="VLF64_01065"
     CDS             complement(982..1656)
                     /gene="recO"
                     /locus_tag="VLF64_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HSW90566.1"
                     /translation="MSSERTTAIILRRTNYGEADRILRLLTPLGQRNVIARGVRKEKS
                     KLAGAIELFAISDIVITSGRGDLGILTSARMQVFYRHILEDYDRLQFAYEAINLVAKA
                     SESIDEPEWYDVLAEVYAGLDMFTIPLQLTQVWLYLHYAELTGYGLNLECDVTGAQLD
                     AERTYMYDVNEKGLRPAEQGDIHAGHIKLLRLIGAKPITAVAQVGGVEDVLSDCWLIA
                     RQHAAV"
     gene            complement(1653..2021)
                     /locus_tag="VLF64_01070"
     CDS             complement(1653..2021)
                     /locus_tag="VLF64_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSW90567.1"
                     /translation="MPAKRVLVIEDDQWFLSLVKKTLEREKITVVTASNTIEGMVAIN
                     EMVPDVIILDFFMPGPNALVLLHELQSYGDTAKVPVILCTNNASDIPKDTLSAYGVVS
                     TLDKTTMYPDDIAVAVRKAI"
     gene            complement(2021..2740)
                     /locus_tag="VLF64_01075"
     CDS             complement(2021..2740)
                     /locus_tag="VLF64_01075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSW90568.1"
                     /translation="MEGSRQQTNRLEGAVPTAPSFVAAAHELKSPLALVRQLALHLES
                     GEVTGGELQEIAHRITLTSERAIRLTTDITKSSRLEDGLFSLEPLNPFVLCQEVVDEM
                     KPLYAAHGRTIVLSSRARSLLALANRDLLRRILLNFADNALHYSGDGPVTITASLRDG
                     GSCIRLGVRDYGPALPSVMRKNVGQKSAHVHSRPESSGLGIYIARQFADAMHARIGAT
                     SHRDGATFYVDIGTSSQLRLL"
     gene            complement(2777..3436)
                     /locus_tag="VLF64_01080"
     CDS             complement(2777..3436)
                     /locus_tag="VLF64_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90569.1"
                     /translation="MKNRTRETGAVNASIFAIVALILLVLVFGSFSIWAYMNYMDQKK
                     DVDSKIANAEAKAILDKATKDEAAFAAREKEPVRSFVGPSDYGALKFDYPKTWSAYQA
                     TDVSKGGGVTYEAYLNPILVPPVSDSTKFALRITIEQKTYDQSVAAYQKLITKGDLKS
                     SVYSDGKMTGTKIVGNFNKDIYGTAILLKMRDRTLTLRTDGDVFNDDFAALLKTVSFN
                     Q"
     gene            complement(3458..3967)
                     /locus_tag="VLF64_01085"
     CDS             complement(3458..3967)
                     /locus_tag="VLF64_01085"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSW90570.1"
                     /translation="MWLLIAAGIVFFVGFVAFTGAPYVPSRRADVIRVFTELYPLTTE
                     DVVVDIGSGDGIVLRTVSKLGAQGVGYEIHPMLVLISRMLSRGDKKVRVRLANFWRVP
                     LPDETTAVYTFGEARDIAKMYARVQQEATRIGWPLMFISYAFSIPGITADASDKSYFL
                     YKVKPLQES"
     gene            complement(3967..4173)
                     /locus_tag="VLF64_01090"
     CDS             complement(3967..4173)
                     /locus_tag="VLF64_01090"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF014648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HSW90571.1"
                     /translation="MSKQSEQKSVSEKMNELSELVAWFEGDEFELEQALNTYSKAEKL
                     AAEIEHDLTNLKNEITVLKEKFDA"
     gene            complement(4157..5257)
                     /gene="xseA"
                     /locus_tag="VLF64_01095"
     CDS             complement(4157..5257)
                     /gene="xseA"
                     /locus_tag="VLF64_01095"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HSW90572.1"
                     /translation="MASDQTPKFSVSDFIAVLNQSLEYSFGTVEIEGEVASFKVNQGK
                     WVFFDIKDATGSVNCFMSLFQLRVPVEDGMKVVVRAVPKITAWGKFSLTIQSIRPSGE
                     GSLKKSFEMLKAKLDKEGLFSLDRKRPLPEIPAHIAVISSTSAAGYADFIKILNDRWG
                     GLQVDVAHVQVQGETAPEQMMRAIAYFNQQEVLPEVLVIIRGGGSADDLAAFNDEPLV
                     RAIAASRIPTIVGVGHEVDVSLADMVADVRAATPSNAAQLVVPDRKELTHTLGQLVRR
                     ILVRIDGMVDDYLKDTNAMLRQGWTVLDARVDLLQSQLVTSRQVMAAYDPKAVLSRGY
                     GIVRGEVASGGMITIEQQERIITAEVKDVKTI"
     gene            complement(5229..>5947)
                     /locus_tag="VLF64_01100"
     CDS             complement(5229..>5947)
                     /locus_tag="VLF64_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HSW90573.1"
                     /translation="YGVSTGKATMAQGVRYVSGFAAMVTLLIVAVFWVTIDGTVNRWI
                     ILMSSMLVTLMIVTIIGSGFLFSSVTRMHNIAKKITRLCNRIVRWATLGRVAELISAK
                     AATEFLDDMHDDYVALMREKRLLVRPYLWGLFFTATEIAIFWIVFWGLNTVVNPAPIL
                     IAYGLASLAGFIVVTPGGAGAYEAIMVAVLAIAGMQKSEAIAGILLARVIILLVTIVV
                     GWILYQQVVMKHGKRPDPEI"
BASE COUNT         1606 a         1664 c         1150 g         1527 t
ORIGIN      
        1 aaatcagtac ggtacgcgag tcccatcaat tcttcacgtc caatcgggaa atcaaattaa
       61 aaatctatag tacgcttact atagtgagcg cggtcatctt caggaacttc tagctcgtga
      121 atgtcctccg cgggtaagcc aagtgccgca aaccattctt tacactgtgc gacccactgc
      181 tcaaatgctt cctgccagtg ttcagggtca acaaaatact caatctccat ttgttcgaat
      241 tcacgactgc ggaagataaa gtcacgtggg ctaatttcat tacgaaacgc cttaccttgc
      301 tgcgcgatac cgaatggcaa gtctgggtaa aaactatcaa ccacgttctt gtagttcgta
      361 aagataccct gcgccgtctc tgggcgaaga taactgattg agctctcatc agcaacgggg
      421 ccaacattcg tcttaaacat catattaaac tgctttggtt cgccaaatgt accgctcata
      481 ccgcaagttg ggcacttatt agggtcttcg agcttatccg ctcggtgacg cgcatgacag
      541 ttactacatt caacaagcgg gtcggtgaat gtatcgacgt gcccacttgc ctgccatacc
      601 ttttggttca tcaaaattgc tgcatccaca ccatacatat cgtctctggc ctcaacaaac
      661 atcttccacc aaagctgcat aatattgcgc ttcaatgcaa cgccaagtgg gccgtaatcc
      721 catgttccac tcaaaccgcc atagacgtca cttccctgat aaataaatcc tcggcgcttc
      781 gcaaggctaa cgatatcgtc taacttgacg tctgacataa tattctctaa cttcctgcgt
      841 gttgggaaca tgctttagga ttaacttagg gtaattatac cataaaaatc taagcttctg
      901 gtggctgggt tttagatact aaacaatcca cgctggcaca tttgagaatc gagccgccag
      961 agggcttctc cttattctca gctaaaccgc cgcatgctgc cgcgcaatca gccagcagtc
     1021 agatagcaca tcctcaacgc cgcccacttg ggctaccgct gtaattggtt tcgcaccgat
     1081 aagacgtagc aacttaatat gccctgcgtg aatatcaccc tgctcggctg gtctcagccc
     1141 cttttcgttc acgtcataca tatacgtacg ctcagcatca agctgcgcac ccgtcacatc
     1201 acattctaaa ttaagcccat agcccgttaa ttcagcatag tgtaggtaaa gccatacctg
     1261 cgtcaactgt agcggaattg tgaacatgtc caaccctgca tacacttcag ccaacacatc
     1321 ataccactca ggctcatcaa tgctctcgct agccttcgca actaaattaa ttgcctcata
     1381 agcaaactgc aagcgatcat agtcctccaa aatatggcgg tagaacacct gcatccgtgc
     1441 gctagttaaa ataccaaggt cacccctacc tgatgtaata acgatatcgc taatagcaaa
     1501 gagttcaatt gcacctgcaa gcttgctctt ttctttacgc acgccccgcg caataacatt
     1561 tcgttgtcca agtggagtta gtaaacgcaa aatacgatca gcctcaccat agtttgtacg
     1621 ccttagtatg attgccgtcg ttcgctccga gctcatattg ccttccttac tgcaaccgca
     1681 atatcatcag gatacattgt ggttttatca agagttgaca ccacgccata agcactcagt
     1741 gtgtccttcg gaatatcact tgcattattc gtgcaaagta tcacggggac tttcgccgta
     1801 tcaccatacg attgcagctc atgtagcaac accaacgcat ttggccccgg cataaaaaag
     1861 tcgagaataa tcacatcagg caccatttcg ttgattgcca ccatcccttc aatcgtatta
     1921 cttgccgtta ctacagtgat tttttcacgt tccaaagttt ttttaaccag cgacaagaac
     1981 cattgatcat cttcaataac aagcacacgc tttgccggca tcacagtaac ctcaactggc
     2041 ttgacgtacc aatatcaacg taaaatgttg caccatcgcg gtggctcgtc gcgccaatcc
     2101 ttgcatgcat tgcatcggca aattgacgcg caatataaat accaagcccg ctactctccg
     2161 ggcggctatg cacatgcgcc gatttctgcc ctacgttttt acgcatcaca gatggaagtg
     2221 ctggcccata atcacgtaca ccaagccgta tacacgatcc accgtcacgt agtgatgccg
     2281 taattgttac cgggccgtca ccactgtaat gtagggcgtt atcagcgaaa ttgagtagaa
     2341 ttcttctcag tagatcacgg ttcgcgagcg ccaacagtga tcgcgcccgc gacgacagta
     2401 caattgtacg gccatgtgct gcataaagcg gcttcatttc atccaccacc tcttggcaca
     2461 gtacaaatgg attaagtggc tcgaggctaa ataagccgtc ctcaagccgt gatgatttcg
     2521 taatatctgt cgtgagccgt atagcccgtt cgcttgtgag cgtaatgcga tgagcaattt
     2581 cttgcaattc acccccagtc acttcgccgg actcaagatg cagcgccaat tgtcgcacta
     2641 acgcaagcgg agatttcaat tcgtgggcag ctgcaacaaa cgaaggcgca gttggtactg
     2701 cgccttccaa acgatttgtt tgctgtcttg acccctccat acctacatct agtgtagcac
     2761 ggcaacaggt tggctactac tggttgaagc ttaccgtctt gagaagtgca gcaaagtcat
     2821 cgttaaatac gtcgccatca gtacgtagcg tcagagtgcg gtcgcgcatc ttaagtaaaa
     2881 tcgccgtacc atagatatct ttattgaaat taccgacaat cttcgtacca gtcatcttac
     2941 catccgagta cacgcttgat ttcaaatcgc ctttagttat cagtttctgg tacgctgcaa
     3001 ccgactggtc gtaggtcttt tgctcaattg tgatacgtag tgcaaattta gtactgtcgc
     3061 tcactggtgg cacaagaata gggttcaggt aagcttcata ggtcacgccg ccgcctttgc
     3121 ttacatcagt tgcctgatat gcactccaag tttttgggta atcaaacttc aaagcaccat
     3181 agtcagatgg accaacaaaa ctgcgcaccg gctctttttc acgggcagca aatgctgcct
     3241 cgtctttagt cgccttatca agtatcgcct tcgcttcagc attagcaatc ttcgaatcca
     3301 catctttctt ttgatccatg taattcatgt aggcccagat gctaaacgag ccaaaaacga
     3361 gcacgagcaa aataagcgca acaattgcaa agatgctcgc atttactgcc cctgtttcac
     3421 gagtgcggtt cttcatgctt atgattatac gtggctacta gctctcttgt aaaggcttaa
     3481 ccttgtacag aaagtacgat ttatctgatg catcggccgt aattccagga atgctaaaag
     3541 cgtagcttat gaacattaac ggccatccga tccgtgtcgc ctcttgttgc acacgcgcat
     3601 acattttcgc aatatcccgc gcctcaccaa aagtatatac ggcggtcgtt tcatcgggta
     3661 gcggcacacg ccaaaagttt gctaatctca cccgaacctt cttgtcgcca cgcgatagca
     3721 tccgtgaaat caacacgagc atcggatgaa tctcataccc caccccttga gcaccaagct
     3781 tgctcaccgt gcgcaagact atcccgtcac ctgaaccaat atcaacaaca acatcctctg
     3841 tggtaagcgg atacagttcc gtaaatacac gtataacatc agctcggcgt gaaggtacat
     3901 acggcgcacc cgtaaacgcg acaaatccaa cgaagaatac aatgccagca gcaatcaaga
     3961 gccacattaa gcgtcaaatt tctcctttag aacggtaatt tcattcttaa gattcgttaa
     4021 atcatgctca atttcagcag caagtttttc agccttgcta taggtattaa gtgcttgttc
     4081 gagctcaaac tcatcaccct caaaccacgc aacaagttca ctgagttcat tcattttctc
     4141 tgatacactt ttttgttcag attgttttga catctttcac ctccgccgta ataattcgtt
     4201 cctgttgttc aattgtaatc ataccaccac tagcaacttc cccgcggaca atcccatacc
     4261 cacgcgacag caccgccttc gggtcatatg ccgccatcac ctgtcgcgat gtcacaagct
     4321 gactttgcag caaatcaacc cgagcatcaa gcactgtcca accctgacga agcattgcgt
     4381 ttgtgtcttt aagataatca tcaaccatcc cgtcaatgcg tactaaaata cgacgcacta
     4441 attgccctag cgtatgagta agttcttttc gatcgggtac cacaagctgc gctgcattac
     4501 ttggtgtcgc tgcgcggaca tccgccacca tatctgcgag actcacgtca acttcatgcc
     4561 ccacaccaac aatcgtcgga atacggctcg cggcgattgc tcgtacgagt ggctcatcat
     4621 taaacgctgc caagtcatca gcgctaccac caccacgaat aatcactagc acttcaggca
     4681 acacttcttg ttgattgaag tacgcgattg cacgcatcat ttgctcgggc gcagtctcac
     4741 cttgcacttg cacatgtgca acatctactt ggagcccacc ccaacgatca ttcagaatct
     4801 ttatgaagtc tgcataccca gcagcggacg tactactaat caccgcaata tgcgcaggaa
     4861 tttccggcag tggccgcttg cgatctagtg aaaagagtcc ttctttatca agcttagcct
     4921 ttagcatctc aaagctcttc ttcaagctcc cctcgccact cggacgaatc gattggatag
     4981 tcaggctaaa cttgccccag gcagtaatct ttggcaccgc ccgcaccaca accttcatgc
     5041 cgtcttccac cggtacacgc agctgaaaga gtgacataaa acaattgaca ctacctgtcg
     5101 cgtccttaat gtcaaaaaat acccacttgc cctggtttac cttaaaactc gcgacttcac
     5161 cttcaatttc aactgttcca aagctgtatt cgagcgattg gtttaagacg gcgataaagt
     5221 cgctaacact aaatttcggg gtctggtcgc ttgccatgtt tcatcactac ctgctgatac
     5281 aaaatccaac caaccacaat cgtcacaagc aaaataatca cacgtgccaa aagaattcct
     5341 gcaattgctt cactcttttg catccccgca attgcaagta ctgctaccat aattgcctca
     5401 tacgcacccg ccccgccagg ggtaacaacg atgaatccag ctaaactagc taggccatat
     5461 gcaatcaaaa ttggtgcagg gttcaccacg gtattaagcc cccagaatac aatccagaaa
     5521 atcgcgattt ctgtcgctgt aaagaacaaa ccccacaaat atggcctcac aagcagacgc
     5581 ttctcgcgca taagcgccac atagtcatca tgcatatcat cgagaaattc agttgcggcc
     5641 ttagcggaaa tgagctcagc aactcgcccc aacgttgccc atctcacaat tctgttacaa
     5701 agccgtgtaa ttttcttcgc aatattgtgc atacgcgtta cgctactaaa caaaaagcca
     5761 ctaccaatga ttgttacaat catcagcgtt actaacatgg agctcatcag aataatccag
     5821 cggttcactg taccatcgat cgttacccaa aacacggcca caatcaaaag tgtcaccatt
     5881 gctgcaaagc ccgagacata tcgcacgccc tgtgccattg tcgccttgcc agttgataca
     5941 ccgtatt
//
LOCUS       DATIIX010000035         7393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_397388, whole genome shotgun sequence.
ACCESSION   DATIIX010000035 DATIIX010000000
VERSION     DATIIX010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7393
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397388"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VLF64_01105"
     CDS             <1..300
                     /locus_tag="VLF64_01105"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90574.1"
                     /translation="GALALAVATFQAVERVGLPEANYNLYHCATALARSEKSREVSEL
                     MYTAKDLAKGFPDAKVPLHLRNAPTKLMKDLGYNKNYKWEAGFHPENGFLPKDVR"
     gene            complement(297..1967)
                     /locus_tag="VLF64_01110"
     CDS             complement(297..1967)
                     /locus_tag="VLF64_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HSW90575.1"
                     /translation="MTKGRASMASKPWSEVNAIYQIYPRSFKDSNSDGIGDLRGVIEK
                     LDYIHGQKDSLGIDAIWFSPIFSSPMADMGYDVSNYCDIDTLFGTLDDFKELVDKAHE
                     RNINVMIDFVPNHTSNQHPWFLASRKDDKDKKDYYVWRNAKRDGSAPNNWLSIFGGSA
                     WEWDDERKQYYLHTFLKEQPDLNWDNPMVRDEMKRVVRFWMELGVDGIRADAVRWISK
                     DPELHDDPVNTEWKPNSTNNTFDSLRHKYSRFWKNLFPYLRELTDVMAEFDNRIMIFE
                     DYPDDNYSTKSQYLGFYNVDPKIGIPFNFEGLWAKFDAATFRSFITEFQGMIDPEAHR
                     PIYCFGNHDQWRMVSRYGSDEKARLVALLQMTLPGLPTLYYGDELGMPNTPVTHEEIR
                     DLSAFSSGDDSVSRDPERTPMQWDKGQYAGFSDVKPWLPVGRSVSTHNVAVELNEPDS
                     FLSLYRRLLRLRSRHEILRSGSYEAFGDTDKNVFVFSRRFGDEHVFVALNFSSKKQKF
                     TLPHKARVICCTHPVDYPEIDKNHTVTLRPYEGVVVECSKHPLQQVTD"
     gene            complement(1936..3555)
                     /gene="treF"
                     /locus_tag="VLF64_01115"
     CDS             complement(1936..3555)
                     /gene="treF"
                     /locus_tag="VLF64_01115"
                     /inference="COORDINATES: protein motif:HMM:NF009773.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HSW90576.1"
                     /translation="MFNRWRPRKQNKLVTKSADTVKAAVVQAAGTLMPVSASPEEALK
                     ALFDEVQQRRVYPDGKTFADMIPRQRVRALLRTYETERSQPNFDLHAFVDKHFYEFLP
                     EHGEVNIQGSSNTREHVRNLWPQLQRRNRRDRGSLIAIPHEYVVPGGRFSEQFYWDSY
                     FIMLGLAADEHWPIIEGMMKNYSFMIFKFGRIPTANRTYFLTRSQPPFFAQMVRLLAK
                     HNGRTRTLAEYLPSLLLEYRFWMKGRREVVADADLQAKLRTVCLPNGVVLNRYFDDKS
                     TPRPESHREDTETAAFAATQNKRKVYRDLRAAAESGWDFSSRWFTDPNKIETIYTTDV
                     VQVDLNCLLYQLEMTIADAYMALKQPLLARKFHQAAEKRADAIRRYCWNPETQFFEDY
                     NFRTGTTTGKATLAGAFPLYVKVATSEQAAMVAKRLEQDFLKKGGLVTTLETTAQQWD
                     APNGWAPLQWTTIQGLREYGYHGLANTIRDRWLKLNERVYETAQKMVEKYDVVTAQPG
                     GGGEYPLQDGFGWTNGVYAALYDERQGEHGK"
     gene            complement(3649..5466)
                     /gene="typA"
                     /locus_tag="VLF64_01120"
     CDS             complement(3649..5466)
                     /gene="typA"
                     /locus_tag="VLF64_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HSW90577.1"
                     /translation="MTDHKFIRNIAIIAHVDHGKTTLVDGLLKQSKTFRDNQAEMGQH
                     LIMDSGEQEHERGITITAKQTSIYHGDYKINIIDTPGHADFSGEVERTLNMADGVLLI
                     VDAQEGPMPQTKFVLSKALELGLKPVVVINKIDKPARRIAEVEDELSDLFLELATDDS
                     QLQYPIYFAIGREGKAWKSMPENIEQHADLEPIFDAIVNDIPAPTVDESGALQLLVTS
                     LTYDSFLGKYAIGRITRGAVKRGPVVLIKRDGAQVSSKIEKIFGYRGLQKEEIEEASA
                     GDIVALIGVGDAHIGETIADKDAPEALPTIDIEAPTLSMYLGPNTSPMKGKEGEFTTS
                     RQIGDRLKKELETNVSLRVEENGIGFTVSGRGELHLSVLIETMRREGFEFEVGRPQVV
                     TITEDGVEKEPVEELYIEVAPEFVGAVSQELGARRAEMRAQEQTASGTTRLDYILPTR
                     ALIGLRNILLTQTKGTVIMNSLPHGYQPVSGKLPKNRNGVLISFEAGTTTPYALQTAE
                     ARGELYVGAGTVVYAGMIIGLNRRSEDMEINVCKAKHLTNMRSSSSDGVVQLTAFTDL
                     SLEQCIDFIEDDELLEVTPKSLRLRKRYLDANERKRHQK"
     gene            complement(5535..5969)
                     /locus_tag="VLF64_01125"
     CDS             complement(5535..5969)
                     /locus_tag="VLF64_01125"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HSW90578.1"
                     /translation="MNDILTRDNLYWMLIQVAFRSKHGLMKLAEKYDLTGVQLYSLGA
                     MNPHEAIPMNELSFILLCDASNVTGIVDRLLTQGYIKREENPTDRRVKLITLTPEGEK
                     LRAALLEEASRYELPEMKRLTNNQRNELKSMLSLILQSSEKP"
     gene            6077..>7393
                     /locus_tag="VLF64_01130"
     CDS             6077..>7393
                     /locus_tag="VLF64_01130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSW90579.1"
                     /translation="MKQQSRWIVLALLALAQFMVVLDTSVANVALPAIQKTLGFDPSA
                     LQWVITTYALTFGGTLLLGGRAADLFGRRRTLLIGISGFTLISLLIGLAQSPLQLIVL
                     RALQGMSAAFMSPSALSIVLTTFREGKARNTALGVWSAVAAGGAAAGLVIGGVFTEYL
                     NWRWNFFINVPVGIFAIAGILKYVPAHASTSSHRNLDLRGALLVTSGLMSLVYGLTKA
                     PSWGWISIATLGTLTASAVLLAGFIWNESRANHPLMPLSIFKIRNVAGANALMTPITA
                     GMLGQFFLLSLYLQSVMHYKPVEAGVAFLPFPIVLAVVANTVPRFMQRVGFKKFLMAG
                     SILLGFGLLWLSQITVTSTYLTGILPAVILMAAGMGMSFISVNIAATSGVHAEQTGLA
                     SGMLNTSQQMGGALGLAILSGVASSTAKSSGLDPISALVAGDKRAFL"
BASE COUNT         1735 a         1829 c         1732 g         2097 t
ORIGIN      
        1 ggtgcgctcg cgcttgccgt tgcgacattc caggcggttg agcgcgttgg gctacccgaa
       61 gcaaattaca atctttatca ctgtgccaca gcactcgccc gtagtgagaa atcacgcgaa
      121 gtaagtgaac ttatgtatac agccaaagac ttagcaaaag gattccctga tgctaaagtc
      181 cccctgcatc tccgcaacgc accaacgaaa ctcatgaaag atttaggcta taataaaaac
      241 tacaaatggg aggctggatt ccatcctgag aacggatttc tgccaaagga tgtacgctag
      301 tctgtaacct gctgcaatgg gtgcttgctg cactcaacta ctacgccctc gtatgggcgg
      361 agtgtaacgg tgtggttttt atctatctcc gggtagtcga ccgggtgcgt acagcagata
      421 accctagcct tgtgtggcaa tgtgaacttt tgctttttgc tactaaaatt aagcgcgaca
      481 aatacatgct catcaccaaa tctacgtgag aaaacaaaga catttttatc tgtgtcgcca
      541 aacgcttcgt atgatccact gcgtaagatt tcgtgacgcg aacgtaagcg tagtaatcga
      601 cgatagaggg acaagaatga gtctggctcg ttgagttcca ctgcaacatt atgcgtactg
      661 acgcttctgc caactggcaa ccaaggtttc acgtcactga atcctgcata ttgccctttg
      721 tcccattgca tcggcgtgcg ttcagggtcg cggctaacgc tatcatcacc cgagctaaag
      781 gcagacaaat cacggatttc ctcgtgagtc actggggtgt ttggcatgcc aagttcgtcg
      841 ccgtaataga gcgtcggtaa accaggcaac gtcatttgta gcagcgcgac cagccgcgct
      901 ttttcatcgc tgccatagcg gctcaccatg cgccattgat cgtggttacc aaagcaataa
      961 attgggcgat gcgcctctgg gtcgatcata ccctggaatt ctgtaataaa gctgcgaaat
     1021 gttgcggcat caaatttcgc ccataagcct tcgaaattaa acggtatgcc tatttttgga
     1081 tcaacattat aaaatcctaa gtactgcgat tttgttgagt aattatcgtc tgggtaatcc
     1141 tcgaagatca taatgcgatt atcgaactct gccatcacgt cagttaattc gcgtaaatat
     1201 gggaaaaggt ttttccagaa acggctatac ttgtggcgca aggaatcgaa ggtattgttg
     1261 gtactgtttg gtttccattc agtatttact gggtcgtcgt gaagctcagg atctttactt
     1321 atccagcgta ctgcatcggc gcgaatacca tccacgccaa gctccatcca aaaccttacc
     1381 acccgcttca tctcatcgcg caccattggg ttatcccagt ttagatcagg ctgctctttg
     1441 aggaatgtat gcagataata ctgcttgcgc tcgtcatccc actcccatgc agagccgcca
     1501 aaaatactaa gccagttatt tggcgcgcta ccgtctcgct ttgcgttacg ccatacatag
     1561 taatcttttt tgtctttatc gtctttacgg gaagccaaga accatggatg ttgattgctt
     1621 gtatgatttg gtacaaaatc gatcatcacg ttaatattac gctcatgagc cttgtcgaca
     1681 agttctttga aatcatcgag tgtcccaaat aatgtgtcga tgtcgcaata gttactgaca
     1741 tcatatccca tatcggccat tggagatgag aaaattgggc taaaccaaat agcatcaatg
     1801 cctaatgagt ctttttggcc gtgaatataa tcgagtttct cgattacgcc gcgcaaatca
     1861 ccgataccat cactgttgct gtctttgaaa ctgcgtggat agatctgata aattgcattc
     1921 acttcactcc atggtttact tgccatgctc gccctgcctt tcgtcataga gcgcagcata
     1981 gacaccattc gtccagccga atccgtcttg caatggatat tcgccgccac caccgggctg
     2041 tgctgttacg acatcatatt tttcgaccat tttttgtgcg gtctcgtata ctcgctcgtt
     2101 tagtttaagc catctatcgc gaatcgtgtt cgcaaggcca tggtatccat attcgcgtag
     2161 accctgaatt gtcgtccact gcaatggcgc ccagccattt ggcgcgtccc attgctgagc
     2221 ggttgtttca agtgttgtta cgagacctcc ctttttcagg aagtcttgct caagacgttt
     2281 tgccaccata gccgcctgct cgcttgtggc aactttgaca tagagtggaa acgcgcctgc
     2341 aagtgtcgct ttgcctgttg tcgtacccgt cctaaaatta taatcttcga agaactgtgt
     2401 ttcagggttc cagcaatagc gacggatggc atcggcgcgc ttctctgccg cctgatggaa
     2461 tttgcgcgca agcaatggct gctttaatgc catgtaggca tcggctattg tcatttcgag
     2521 ctggtagagc aagcagttta aatccacttg aaccacatca gtagtatata tagtctcaat
     2581 cttatttgga tcagtaaacc aacgactgct gaagtcccag ccactttctg ctgcggcgcg
     2641 tagatcacgg taaactttac gtttattctg agtagcagcg aaggctgccg tttctgtatc
     2701 ttcacgatgt gactctgggc gcggcgtcga cttatcgtca aaatagcgat ttagcaccac
     2761 accatttggc aaacatactg tacgcagctt ggcttgtaaa tcagcatctg caacaacttc
     2821 acgcctgcct ttcatccaga agcgatattc aagcaataat gacggcaagt actcagcaag
     2881 tgttcgcgtg cgaccattat gcttggccaa taatcgtacc atttgtgcaa aaaacggtgg
     2941 ctggctcctt gtcaaaaagt atgttcgatt tgccgttgga atacgtccga acttaaatat
     3001 cataaagcta tagtttttca tcatgccttc gatgattggc caatgctcat cggcggcgag
     3061 gccaagcata ataaaatatg aatcccagta gaattgttcg ctgaagcgcc cgcctggcac
     3121 gacatattcg tggggtatgg caataagcga tccgcgatca cggcgattac gacgttgtag
     3181 ctgcggccat aagtttcgta catgctcgcg cgtattcgaa ctgccttgta tgtttacctc
     3241 accatgctcg ggcaaaaatt catagaagtg tttatcgaca aaggcatgca aatcgaaatt
     3301 aggctgtgag cgctcggtct catatgtgcg cagtaacgca cgcacacgct gacgggggat
     3361 catatcggca aatgtcttgc cgtctggata gactcttcgc tgctgaacct catcaaataa
     3421 cgccttgagg gcctcttcgg gactcgcgct cactggcatc aaagtgcccg cggcttgtac
     3481 gacagcagct ttgactgtat cggcactttt tgtgacaagt ttgttttgtt ttcgaggtcg
     3541 ccagcggtta aacatatccg tattatacgg ttttcacaca tctgttacaa cctgcaaaac
     3601 gcactaaaaa agagatgggc gcatatccat ctcttttcga actttgactt atttctggtg
     3661 gcgtttgcgt tcgttcgcgt cgaggtaacg cttgcgaagg cgaaggctct ttggtgttac
     3721 ttcgagcaac tcatcgtctt cgataaagtc gatgcactgc tcgaggctga ggtcagtaaa
     3781 tgccgtcagc tgcaccacgc catcacttga acttgagcgc atatttgtaa ggtgttttgc
     3841 cttacaaacg ttgatttcca tgtcttcgct tcgacggttc aaaccgatga tcataccggc
     3901 atatacgacc gtccctgcgc caacatacaa ttcaccacgc gcttccgctg tctgcagtgc
     3961 gtatggagtt gtcgtaccgg cttcaaatga aatcaacacg ccattgcggt tctttggcaa
     4021 tttgccgctt accggttggt agccgtgtgg caaactgttc ataatgaccg ttcccttagt
     4081 ttgggtcagt aagatattgc gaagaccgat aagagcgcgg gttggcaaaa tatagtcaag
     4141 gcgggtcgtg ccacttgcgg tttgctcttg tgcgcgcatc tctgcacggc gggcaccaag
     4201 ttcttggctg actgcgccaa caaattctgg ggcaacctcg atgtagagct cctcaactgg
     4261 ttctttctca acgccatctt ctgtgattgt cacgacttgt gggcgaccta cttcgaattc
     4321 aaagccttcg cggcgcatcg tttcgataag cacagaaaga tgtagctcac cgcggccact
     4381 taccgtgaag ccgataccgt tttcttcgac acgaagtgat acgtttgttt cgagttcttt
     4441 ctttaggcgg tcgccaattt ggcgtgaagt tgtaaattca ccttctttac ccttcattgg
     4501 gctcgtgttt gggccaagat acatgctaag ggttggcgct tcgatatcaa tcgtcggcag
     4561 cgcttctgga gcatctttgt cggcaatcgt ttcgccgata tgagcgtcgc ccacaccaat
     4621 aagcgcaacg atgtcaccag cactcgcttc ttcaatttct tctttttgca ggccgcggta
     4681 accaaagatt ttttcaatct tgctacttac ctgtgcgcca tcgcgtttga tcaacacaac
     4741 tgggccgcgt tttacagcgc cgcgagtgat acgcccgatt gcgtacttac caaggaatga
     4801 atcataggta aggctcgtta cgagaagttg caacgcgcca ctctcgtcaa ctgtaggagc
     4861 tgggatgtca ttcacaattg catcaaaaat tggctcaaga tcagcgtgct gctcgatgtt
     4921 ttctggcatc gatttccatg ctttaccttc gcggccgatt gcaaaataga tagggtactg
     4981 caattgactg tcgtcggtag caagttcgag gaataagtca gataactcgt cctcgacttc
     5041 agcaatacga cgcgccggct tatcaatctt gttgattaca acaactggct tcagaccaag
     5101 ttcaagcgcc tttgaaagca cgaatttcgt ttgtggcatt gggccttcct gggcatcaac
     5161 gataagaagt acgccgtctg ccatgttaag ggtgcgctct acttcgccgc taaagtctgc
     5221 gtgacctgga gtgtcaataa tattgatttt gtagtcacca tggtagatac ttgtttgctt
     5281 ggcagtgatc gtgatgcctc gttcgtgctc ctgttcgcca ctgtccataa taaggtgctg
     5341 acccatctct gcctgattgt cacggaaggt cttactttgt ttaagaagac catcaacgag
     5401 agtcgtttta ccgtggtcca cgtgtgcgat aattgcgata ttgcgaatga atttgtgatc
     5461 tgtcataaaa ataatgcccc tccggggaca attcctttac gctattgtac catacttacg
     5521 ctaaaattaa aagcctatgg cttttctgag gactgtagga taagagacaa catcgacttg
     5581 agctcgttgc gctgattatt tgtgaggcgc ttcatttcag gcaattcgta tcgtgaggct
     5641 tcctcgagaa gagcggcacg gagtttttca ccctcagggg taagtgtaat taactttacc
     5701 cgacgatcag ttggattttc ttcacgtttg atatagccct gtgtcaaaag acggtcgacg
     5761 atgcccgtta cgtttgacgc gtcacaaaga agaataaaag aaagctcatt catagggata
     5821 gcttcgtgtg ggttcattgc gccaagacta tatagctgca cgccagtaag atcatacttt
     5881 tcagctaatt tcatgaggcc atgttttgaa cgaaaagcaa cctggatgag catccagtag
     5941 agattgtctc gtgtcaaaat atcgttcata cctgttattc tagtgcattt tacttttttt
     6001 agcaaattac ttgacttatt caactgtatt gaggtacgat agttgaggtt ttaaagtaat
     6061 ttatgaaagg aacttcatga aacaacaatc acgttggatt gtgctcgccc tcctcgcgct
     6121 agcgcagttc atggttgtcc tcgatacgtc cgttgcgaat gttgccctcc cggccattca
     6181 aaagacgctc gggttcgacc catcggctct gcaatgggtt atcactacct atgcactcac
     6241 gtttggcggt acactactac ttggcggacg agcagccgac ctctttggtc gtcgtcgcac
     6301 attattaatt ggtattagcg gttttaccct tatatcatta cttattggtt tggctcagtc
     6361 gccgcttcag ctcattgtgc tccgtgcttt acagggcatg agtgcggcat ttatgtcacc
     6421 gagcgcactc tcgattgtgc tcacgacatt ccgcgaaggc aaagcgcgca atactgccct
     6481 cggggtatgg tcggcggttg ccgcgggtgg tgctgcagcc gggctcgtta tcggtggtgt
     6541 attcaccgaa tatctgaact ggcgctggaa cttctttatc aatgtgcctg ttggcatttt
     6601 tgcaattgct gggattttga aatacgttcc cgcgcatgcg tcaacttcct cgcatcgtaa
     6661 ccttgatttg cgtggtgcac tactcgtgac atcagggctt atgagtctcg tctacggact
     6721 cactaaagca ccgagttggg gctggataag catcgcaacg cttggtacgt tgaccgcaag
     6781 cgcagtactc cttgcaggat tcatctggaa cgagtcacgc gcaaaccacc cacttatgcc
     6841 actttcaatc tttaaaatcc gaaacgttgc gggcgcgaat gctcttatga ccccgattac
     6901 agcaggcatg cttggtcagt tcttcttact ttcgctgtac ttgcaaagtg ttatgcacta
     6961 caagccagta gaggccggag ttgcgttcct tcctttccct atcgtccttg cagttgtcgc
     7021 gaataccgta cctcgcttta tgcagcgtgt tggctttaaa aagttcttga tggctggctc
     7081 gattttgctt ggctttgggc ttctatggct tagtcaaata accgtcacaa gcacctacct
     7141 tactggcatc ctccctgccg tcatcttgat ggctgctggt atgggcatga gcttcatctc
     7201 ggtaaatatt gctgcgactt ctggggtgca tgcagaacaa accggtttgg catcgggcat
     7261 gcttaacacc tcgcagcaga tgggcggtgc actcggtctc gcgattctat ctggcgtagc
     7321 ctcatcaacc gctaaaagtt ctgggctcga cccaatctca gcactcgttg ctggtgacaa
     7381 gcgtgcattc tta
//
LOCUS       DATIIX010000036         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_8544, whole genome shotgun sequence.
ACCESSION   DATIIX010000036 DATIIX010000000
VERSION     DATIIX010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8544"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VLF64_01135"
     CDS             <1..862
                     /locus_tag="VLF64_01135"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HSW90580.1"
                     /translation="IEAVAEEEMSIAQFSRTVLINRDDVEGRFAAYEINPNFSTYGTT
                     GAAEYRFEQQAFTIEQGYVGNIIAASAAPFPATIKVVGEHSLRPVMGAVAAALLFGLS
                     PDDVANGLALIKPVPGRMNLLRGIDGTKIIDDTYNSSPAAASAAIQTLYSFDDVPQRI
                     LVLGDMRELGSSSQREHEKLGAICDPNLLAWVVLVGPDCTKYLAPIARGRGCQVHIAP
                     DAIRAGEFVRSVAIEGAAILVKGSQNTIFLEECVKILCDMNEDVELVRQSASWMKVKD
                     EHFSRYTDPK"
     gene            889..>2140
                     /gene="leuS"
                     /locus_tag="VLF64_01140"
     CDS             889..>2140
                     /gene="leuS"
                     /locus_tag="VLF64_01140"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HSW90581.1"
                     /translation="MKRYNPTEIEPKWQKVWADEHRYQAPDASDKPKYYVTSMFPYPS
                     GAGMHTGHFFEHSIVDAEARFRRSLGFSVTYPMGWDSFGLPAENYAIKTGRQPAETTK
                     ENIANFKNQLTRVGASIDWSREINTANPEYYKWTQWIFTQLFERGLAYQKEANQWWCP
                     KDKTVLANEQVINGGHCWRCDSLVIKKPMKQWFFKITDYADALLEEIPALDWPQKIKT
                     AQENWIGRSEGAEINYAINGRDDEITVFSTRPDTIFGATFIVLAPEHPLARELVSDET
                     REMVNAYIDEAIKRSEIERSSENKDKTGVFTGSHAINPANGEKVQIWIADYVLGGYGT
                     GAVMGVPAHDERDFAFAEAFKLPVIEVVERPDNSSEECYHGEGTLINSGSFTGQASSD
                     AREAIVAWLEQEGHGHSKVTYKMRD"
BASE COUNT          577 a          515 c          548 g          500 t
ORIGIN      
        1 catcgaagcg gtcgccgaag aagaaatgtc gatcgcacag ttctcgcgta cggtgcttat
       61 taaccgtgat gatgtcgagg gtcgattcgc tgcctacgaa atcaatccaa actttagtac
      121 gtacggcact accggtgctg cagaataccg atttgaacaa caggcattta ccattgaaca
      181 agggtatgtg ggcaatatca ttgcagctag cgcagctcca ttccccgcga cgatcaaggt
      241 tgttggcgag cacagtctgc gcccagtaat gggtgcagtt gctgcggcat tgctattcgg
      301 tctatcgcca gatgatgttg caaacgggct tgcactcatc aagccggtcc ctggtcgcat
      361 gaacctactt cgtggtatcg atggcacaaa gatcattgat gatacttaca attcgagtcc
      421 ggccgcagcg tctgcagcaa tccagaccct ctattcattc gacgatgtcc cccagcgtat
      481 tttggtgctt ggagatatgc gtgagctcgg cagctcaagc cagcgagagc atgaaaaact
      541 tggtgcaata tgtgacccta atttactcgc ctgggttgtg cttgtcgggc ctgattgcac
      601 taaatacctc gcaccgattg cacgcgggcg tggttgccag gtgcatatag cgccggatgc
      661 aatccgcgct ggtgagtttg ttcggtcggt cgccattgag ggtgcggcga tccttgtaaa
      721 gggatctcaa aacaccatct tccttgaaga atgtgtgaaa atattgtgtg atatgaacga
      781 agatgtcgag ctcgtacgtc aatctgctag ctggatgaag gtcaaagatg agcatttttc
      841 acgctacacc gaccccaaat aatacctccg atggtatact agtaggtaat gaagcgttat
      901 aaccctaccg aaatcgagcc aaaatggcaa aaagtctggg ccgacgagca tcggtaccaa
      961 gctcctgatg cgagcgacaa accaaagtac tatgtaacaa gcatgtttcc atacccgagt
     1021 ggtgctggta tgcacacagg gcatttcttc gaacactcga ttgtcgatgc cgaggcaagg
     1081 ttccgtcgta gccttggctt tagcgttacc tatccaatgg gttgggatag ctttggcttg
     1141 cccgctgaaa attatgccat caagacgggc cgtcagccag ccgagaccac taaggaaaat
     1201 atcgctaact tcaaaaatca gcttacacgt gtcggcgcaa gtatcgattg gtcgcgtgaa
     1261 atcaacacag ccaacccaga atactacaaa tggacacagt ggatttttac tcagctattt
     1321 gagcgcggtt tagcatacca aaaagaagcc aatcaatggt ggtgccctaa agataaaacc
     1381 gtccttgcaa atgaacaggt aataaatggc ggccactgtt ggcgctgtga ctcacttgtc
     1441 atcaaaaagc caatgaaaca atggttcttc aagattaccg actacgccga tgcattgctt
     1501 gaagaaatcc ctgcgcttga ttggccgcag aaaatcaaga cagcccaaga gaactggatc
     1561 ggccgcagtg aaggggctga aattaattat gctattaatg gccgtgatga cgaaatcacg
     1621 gtgttctcaa cacgcccaga tacgattttt ggcgcaacat ttattgttct tgcgcccgag
     1681 catccacttg cgcgcgagct cgtgagcgac gaaacacgcg aaatggtaaa cgcttacatt
     1741 gacgaagcaa tcaagaggtc tgaaattgag cgttcaagcg agaataaaga caaaacaggc
     1801 gtatttactg gcagtcacgc aatcaacccg gcaaatggcg aaaaagtcca gatctggatt
     1861 gccgattacg tgcttggtgg ctatggcact ggcgctgtaa tgggcgtgcc ggcgcacgac
     1921 gagcgcgact ttgcttttgc tgaagcattt aaattgccag tcatcgaagt tgtcgaacgc
     1981 cccgataata gctctgaaga atgctatcac ggcgaaggta cgcttattaa cagtggctcg
     2041 tttacgggtc aggcaagtag tgatgctcgc gaggcaatcg tcgcttggct cgagcaagaa
     2101 ggtcacggcc atagcaaagt tacctacaaa atgcgcgact
//
LOCUS       DATIIX010000037         3620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_259071, whole genome shotgun sequence.
ACCESSION   DATIIX010000037 DATIIX010000000
VERSION     DATIIX010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259071"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..518)
                     /locus_tag="VLF64_01145"
     CDS             complement(93..518)
                     /locus_tag="VLF64_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HSW90582.1"
                     /translation="MRKRIQSIIAAFILSVGFGVMMLAPTTYADDKKATPPSGTSSDA
                     GCTDAKSCVTNGLQATGGTSSKTSISDIFKTIVNVLLFVIGAISVIMIIIGGIKYTTS
                     NGDSSSVTSAKNTILYAVIGLLVSMFAYAIVNFVVTRFI"
     gene            complement(535..906)
                     /locus_tag="VLF64_01150"
     CDS             complement(535..906)
                     /locus_tag="VLF64_01150"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90583.1"
                     /translation="MKLRLLVIVAMLVGLFAINVVGAPVSSAAVDIFGGCKDGVDCSS
                     VQSKQTLNTQIWNFIRTALTILGGIAVVVIIAGGIMYTTSAGDPSKVTAAKNTILYSV
                     VGLGVAMSAAAIITLVNNYFG"
     gene            complement(906..1196)
                     /locus_tag="VLF64_01155"
     CDS             complement(906..1196)
                     /locus_tag="VLF64_01155"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90584.1"
                     /translation="MITPYLYYAAGLINQKASGIPDATSFSIKNVLNAVYFWAGAIAV
                     IVIIVAGFYYVTSANNSSQIVRAKNAIFSAVIGLVIVLLAFGITNIVISGVS"
     gene            complement(1197..1595)
                     /locus_tag="VLF64_01160"
     CDS             complement(1197..1595)
                     /locus_tag="VLF64_01160"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90585.1"
                     /translation="MMLFTLGAATLAPVVVVGGTAYAACEDGPILGITRWYRGLLDDS
                     CNIKAPGNGNDDLAIFITKIILNVVQAALAIAAYITVFFIIKGGFNYMTSAGSPDGMK
                     SAKTTITNAVIGLVIVLLSAIIVNTIAGVL"
     gene            complement(1619..2014)
                     /locus_tag="VLF64_01165"
     CDS             complement(1619..2014)
                     /locus_tag="VLF64_01165"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90586.1"
                     /translation="MKSKVTLYLTSLLVAVSIGFSIFDQPAAALTCAVLPQSICDKAQ
                     SNQQGGNTSDTSAVLALLQWVIGVLTAGVGVAAVAAFIYAGITYASADGSAEQVKKAK
                     SIMQNTVIGLVTFAVMALALQFIIPGGVF"
     gene            2220..>3620
                     /locus_tag="VLF64_01170"
     CDS             2220..>3620
                     /locus_tag="VLF64_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90587.1"
                     /translation="MTFFSVASLLTPAAYADDGATRNGNGIVYQGNNYTTVTAAEMPS
                     DITNNAPDTTGYRYTDNAGKAYFIFMTGDATKATSGFYESYDFVPPNNYANPSTPTKI
                     TIAADGNQGSTDSSKQESGCDNSTLGGIGWLVCPTVNFIAKGMDKIYNIISGFLEVKT
                     VTSDTNTSIYQLWATVRDIANVAFVIVFMVIVYSQITNLGLSNYSVKNMLPRLIFAAI
                     LVNASFWVCAAAVDASNFLGYSIHNMFVALMDKFSVGSNYSGSTPSWQQVATVALSTG
                     AVTGGGLFIAANTVSGTIFLLIPILLGAVISALIALIILAARQALITILIIVAPLAFV
                     AYTMPNTEKYFNKWRETLMTMLLLFPIFSVIFSGAQIAGMAIVQSANGSLITIILGMA
                     VQVAPIVITPLLIKFSGNIIGRVAGIVNNPNRGLIDRTRNWSKGMAAERKNKVLAGQN
                     RYFNRNPLNNATKALDT"
BASE COUNT         1101 a          872 c          709 g          938 t
ORIGIN      
        1 gtcgcttaat gttaatcttc attattattt aattcctttc gttataggct ttattatagt
       61 tatatcaaaa gattcgccaa ctgttaagca ctttatatga atctggtgac aacgaagttg
      121 acaatcgcat aggcgaacat cgatacaagt aacccaataa ccgcatagag tatcgtattc
      181 tttgcacttg ttaccgatga gctatctcca tttgaagtag tatacttaat accaccgata
      241 ataatcatta tgacggatat agccccaata acgaagagga ggacattaac gatagtcttg
      301 aaaatatcac taatacttgt cttggagctc gtaccgccag tagcctgaag gccattggtt
      361 acacaactct tcgcatccgt acaacccgca tcgctagagg taccgctggg aggagtagct
      421 ttcttatcat ccgcataagt agtaggggcg agcatcatca cgccaaaacc gacactcagt
      481 ataaatgctg caataattga ttgaatcctc tttctcatag tttctccttt acggttatcc
      541 gaaataatta ttaacaaggg tgataatagc tgctgccgac atcgcaactc caagtccaac
      601 gacagagtat agtatcgtat ttttcgctgc cgtcactttg cttggatcac cggctgacgt
      661 tgtgtacata atgccaccag cgataataac gacaacggca atcccaccca aaatagtaag
      721 ggcggtacga atgaaattcc agatctgcgt attaagcgtc tgttttgact gcacagaact
      781 acagtcaaca ccatccttgc agccaccaaa tatatcaaca gccgctgaac ttactggcgc
      841 accgactaca ttaatggcaa aaagtcctac gagcatagct acaataacaa gcagtcgtaa
      901 tttcattatg acactcctga aataacaata ttagtaatgc cgaaagcaag aagcacgata
      961 acgagaccaa taactgctga aaaaattgca tttttagcac gaacaatctg tgaagagtta
     1021 ttagccgagg tgacatagta aaagcccgcc acaataataa caataactgc aatggctccc
     1081 gcccagaaat acacagcatt aagaacattt ttgatactaa agctagttgc atcagggata
     1141 cctgatgcct tctggttaat taaacccgcc gcatagtata aatatggtgt aatcatttat
     1201 agaactcccg caatcgtatt aacgataatc gccgaaagga gcacaataac caaaccaatc
     1261 actgcgttag taattgttgt tttagcgctt ttcatgccat ccggtgatcc agcgcttgtc
     1321 atatagttaa agccaccttt aatgatgaaa aatactgtga tatatgcagc aatcgcaagt
     1381 gctgcctgca caacgtttag aataatcttg gtaataaata tagcaaggtc atcgttgcca
     1441 ttacccggcg ctttaatgtt gcaactatca tcaagcaagc ctcgatacca acgtgtaata
     1501 cccaaaatag ggccatcttc acatgccgcg taagccgtac caccaaccac tacaactggg
     1561 gcaagtgttg cagcgcccaa agtgaatagc atcatcaacg atacaaaaaa cctgcgcact
     1621 agaaaacacc tcccggaata ataaattgca gcgccaacgc catcacggcg aatgtaacaa
     1681 gcccaataac ggtattttgc ataattgatt ttgccttttt aacctgctcg gcactgccgt
     1741 cggcactcgc ataggtaata cctgcataga taaatgcggc taccgcagcg acacccacac
     1801 cagccgtaag aacgccaatt acccactgta aaagagcgag tactgcagat gtatcagagg
     1861 tattacctcc ttgctggttg gactgggctt tatcacaaat actttgcggc aacacagcac
     1921 acgtaagtgc tgctgctggt tggtcaaaaa tgctaaaccc aattgatact gcaacgagta
     1981 gggaggtgag gtagagggtt acttttgatt tcatgatgaa ttccttataa cactatacta
     2041 taggtacagc tttcgtctgt aaagtagtct ctaccataac cgtcttaaat gtggtctaat
     2101 gtattgactt ttatagtcaa aaatggtagt atttaacaga tatgtgggag caaagcataa
     2161 gtgttctgca gggtagatta tcgctgcagc gtttttcagt cttggtcatg gccttcttaa
     2221 tgaccttttt cagcgtagct tctttactaa caccagcagc atacgccgat gatggcgcta
     2281 ctcgaaatgg caacgggatt gtctatcagg gcaataacta cacgacggtc accgccgcgg
     2341 aaatgccaag cgacatcaca aataacgcgc cggacacaac ggggtaccgc tacaccgaca
     2401 acgcaggcaa agcatacttc atctttatga cgggcgatgc aaccaaggca acatcagggt
     2461 tctacgaatc atacgacttc gtaccaccaa acaattacgc aaatccatca actccaacca
     2521 agattacaat tgctgctgat ggaaatcaag gtagcaccga ctcttcaaag caagaatcgg
     2581 gctgtgacaa ctccacactt ggcggcatcg gctggcttgt ctgcccaacg gtaaacttca
     2641 tcgccaaggg catggacaaa atctacaata tcatctctgg atttctagag gtaaaaacag
     2701 tcaccagtga cactaatacc tcaatctacc agctatgggc aactgttcga gatatcgcaa
     2761 acgtagcctt cgttattgta tttatggtga ttgtatactc acaaatcact aatctcggcc
     2821 ttagcaacta cagtgtaaag aacatgctac cacgcctcat ctttgcggca atcttagtaa
     2881 atgcatcatt ctgggtatgt gcagccgcag tcgatgcctc taacttcctt gggtactcca
     2941 tacataatat gttcgtagcc ttaatggata aatttagcgt cggcagcaat tactcaggga
     3001 gcactccttc ttggcagcaa gttgctacgg ttgcactatc aactggtgcg gtgaccggcg
     3061 gcggactctt cattgcagcc aacaccgtaa gtggtactat attccttctc attccaatcc
     3121 tacttggtgc cgtcatatct gcgcttattg cacttattat acttgctgca cgacaagcac
     3181 ttattacaat tcttatcatc gtagcgccgc tcgcattcgt cgcatacaca atgccgaaca
     3241 ccgaaaaata cttcaataaa tggcgcgaga cattaatgac aatgttactg ctctttccaa
     3301 ttttctcagt tatcttcagc ggagcacaga ttgctggtat ggcaatcgtc cagagtgcaa
     3361 acggcagcct tattacaatc atcttaggaa tggccgttca ggtagcgcca atagttatta
     3421 caccactcct tatcaaattc agcggtaata ttatcggtag agttgctggc attgttaaca
     3481 atccaaatcg cggactaatc gaccgtaccc gaaactggtc aaagggtatg gccgcagagc
     3541 gtaaaaacaa agtacttgct ggacaaaatc gctattttaa ccgcaacccg cttaacaatg
     3601 ccaccaaagc acttgatacc
//
LOCUS       DATIIX010000038         3437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_315876, whole genome shotgun sequence.
ACCESSION   DATIIX010000038 DATIIX010000000
VERSION     DATIIX010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315876"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1332
                     /locus_tag="VLF64_01175"
     CDS             <1..1332
                     /locus_tag="VLF64_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HSW90588.1"
                     /translation="IGGTINGSGSLTIRVTRVGDDTALAGIMKLVAEAQRSKSKTQIV
                     ADKAAFYLTFVALGAAIITWIAWAIAGQSAGFILERIVTVLVIACPHALGLAVPLVTA
                     ISTTLAAKNGLLIRQRMALEAARNIDVVLFDKTGTLTKGEQGVVDIVALGDKKELLQL
                     AAAVEDESEHPIARAVVLFAKKQEFSIPKASGFVALSGRGAKAEVNEKIVHIGGPRMI
                     EELSAQVADSLRQTAANASDEGKTVVYVIADRQVLGVIMLADVIREESKEAVATLQAM
                     GKRVAMLTGDAKGVAAWVARELGIEEYFAEVLPEHKAETVKRLQADGSKVAMVGDGVN
                     DAPALTQADIGIAIGAGTDVAIESAGIVLAGSDPRGVAKIVKLSKATYRKMVQNLWWA
                     TGYNALAIPLAAGITSALGFVLSPAFGAVLMSLSTIIVAINAQLLRRLKLN"
     gene            1348..1563
                     /locus_tag="VLF64_01180"
     CDS             1348..1563
                     /locus_tag="VLF64_01180"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HSW90589.1"
                     /translation="MHEHHHDIDKAAIPLERQATCPVTGDMVDMQEAKTAGRVREYNS
                     KTYYFCCNTCVRLFDKNPEKYAAHKSI"
     gene            1726..3297
                     /locus_tag="VLF64_01185"
     CDS             1726..3297
                     /locus_tag="VLF64_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90590.1"
                     /translation="MSELNTVGSHLMVGLSNAVHNEDTETRTQAQAEKVNIVGAGETL
                     TAAYEQLRNAAENTEEHLLVQNAIRRFYKQLFVTRDESLIRASGHEIAIELTYAGYMP
                     NNTLTKDQLEAISELAASHYTAYEHIQKRRGISSDSSIGWILDTLSVGVVRIINSSEK
                     DAAFVSVAYQYFEKVAHSEYTDTSLDADEYGAALYAAVHKALLKSNTAVIRASLLSRY
                     EVSVTDEERYIAYNQRIDMLLRSPVTDKFYHMVDRQGAPLRIVRRMIENKPDFLQLLQ
                     RREAFLDAFEKEVEREYSNIMTRVNRAIVRSVIFLIITKFLIGIAVEVPYDLWASGHI
                     LWQPLLINLCFPPLYMVALRLTLNLPGYANTKALVDRVDAMLYGDEGTILLKRRGATN
                     DRYGSVFSTLYVLFSLVVFGGVVWLLLSLGLELVHIAIFFVFISAASFLGFRLSRLIR
                     ELEVVRSASNGFTFIRDFIYLPFVVVGRWMSEKYAQVNVVALALDMMIELPLKTVLRL
                     IRQWGSFIDDRKDRI"
BASE COUNT          863 a          815 c          869 g          890 t
ORIGIN      
        1 attggtggca cgatcaacgg cagtggctcc ctcactatcc gcgtgactcg ggttggcgac
       61 gacacagcct tagcgggtat tatgaaacta gttgccgaag cccagcgtag taagtcgaaa
      121 acgcagattg tggctgataa agccgccttt tatcttacat tcgtcgcgct tggggcagca
      181 attattacct ggattgcctg ggcgattgcc gggcagtcag cggggttcat actcgagcgt
      241 attgttactg tccttgtgat tgcctgtccg cacgctctcg gactagcggt gccactggtg
      301 acggccatct ccactacgct tgcggctaag aacggcttgc tgattcgcca gcggatggca
      361 cttgaggctg cccgcaatat agatgtcgtc ttgttcgaca agaccggtac acttaccaag
      421 ggtgaacaag gagtggttga cattgttgct cttggtgaca aaaaggagct actgcaattg
      481 gcggcagccg tcgaagatga atcagagcat cccatcgccc gtgctgtcgt actgtttgca
      541 aaaaaacagg aattcagcat accaaaggct agcggtttcg ttgcactttc cggcagaggt
      601 gcgaaagctg aagtaaatga aaagattgta catattggcg gcccgcgcat gattgaagaa
      661 ctatccgcac aagtggctga tagcttacgg caaacagcgg ccaacgcttc tgatgaaggc
      721 aagactgtcg tctacgtcat tgcagacagg caggttctcg gagtaatcat gctggctgac
      781 gttatccgtg aagagtctaa ggaagctgtc gctacactac aggcaatggg taagcgcgtt
      841 gctatgctca ccggtgatgc caaaggtgtg gccgcctggg ttgctagaga gcttggcatt
      901 gaagaatact ttgccgaagt tttgccagag cataaagctg aaacggttaa acggttacag
      961 gccgatggca gcaaggtagc tatggttgga gatggcgtaa atgacgcgcc agcacttacg
     1021 caggcagaca tcggcattgc tatcggcgcg ggtactgacg ttgctatcga atcggcgggt
     1081 attgtacttg ccggtagcga cccacgcggt gttgcgaaga ttgttaagtt atccaaagct
     1141 acctaccgca aaatggtgca gaacctgtgg tgggcgaccg gttacaacgc ccttgctatt
     1201 ccgctagccg caggcataac ctctgcactt ggttttgttt tgtcgcccgc atttggtgca
     1261 gtattgatgt cgctttcaac catcattgta gccattaatg cacagctact acggcggctt
     1321 aaattgaatt aaaggggata gacatatatg cacgaacatc atcatgacat agacaaggcg
     1381 gccatcccgc tcgaacgcca agccacatgt ccagttactg gcgatatggt agacatgcaa
     1441 gaggccaaga ccgccggtcg tgtccgcgag tataatagta aaacgtatta tttctgctgc
     1501 aacacctgcg tacgactttt cgataaaaac ccagaaaaat acgcagcaca caagagcatc
     1561 taaataactc tatcgatagt ttccactcta tggtgatggt gagctgactg caattgctcg
     1621 gcggataaat ggtataggaa tcgaatgcta gttattgtac cattatacgc attggggcag
     1681 cctagatagt gtaattacat ctcttaaagg gtataataat acggtatgtc agagctcaat
     1741 actgtcggca gtcaccttat ggtaggactt tccaacgccg ttcacaatga agataccgaa
     1801 acacgcaccc aggcgcaggc cgagaaggtg aacattgtag gcgccggcga aacgcttact
     1861 gctgcctatg agcaacttcg taatgcagcc gaaaacaccg aagaacattt gctggtgcaa
     1921 aatgccatca gacgattcta taagcaacta tttgttacgc gcgatgaaag cttgattcgt
     1981 gcgagcggtc atgaaattgc aattgagctt acctatgcgg gctatatgcc aaacaatacg
     2041 ctcaccaaag accagctaga ggccatttct gagcttgcag caagtcatta cactgcctat
     2101 gagcatattc aaaaacgtcg cggcatcagt tctgattcgt caattggatg gattctcgac
     2161 actctctcgg ttggtgttgt ccgcattatt aattcatctg aaaaagatgc ggcatttgta
     2221 tcagttgcat accaatattt cgagaaagtt gctcacagtg aatacactga tacctcactt
     2281 gatgctgacg aatatggggc ggcactatac gccgcggtcc ataaagcact cctgaaatca
     2341 aatactgcag ttatccgcgc ctcgctgctg agtcgctatg aagtgtcagt gactgatgaa
     2401 gaacgatata tcgcctacaa tcagcgcatc gatatgttgc tccgctcccc ggtaaccgat
     2461 aagttttatc acatggtcga ccggcagggc gcaccccttc gtattgtgcg gcgaatgatt
     2521 gaaaataagc ccgactttct acagctgctg caacgtcgcg aagcgttcct cgatgcattt
     2581 gaaaaagaag tcgaacgcga atattcgaac attatgacac gagtcaatcg tgccatcgtt
     2641 cgtagcgtaa ttttcttgat tatcacgaag ttcttgattg gcattgccgt tgaggttcca
     2701 tacgacctct gggcgagcgg ccacattctt tggcagccac tactcatcaa cctttgtttc
     2761 ccgccgcttt atatggtagc actgcgcctc acgctgaact tgccagggta tgcgaacaca
     2821 aaggcacttg tcgatcgagt tgatgcgatg ctgtatggtg atgagggcac gattcttcta
     2881 aagcgccgcg gagcaacgaa tgatcgatat ggatcagtgt tctcaacgct gtacgtgctt
     2941 tttagtttgg tcgtatttgg cggtgtcgtt tggttacttc tatcgcttgg actcgaactg
     3001 gtgcacatcg ctatcttctt tgtatttatt tcggccgcaa gctttcttgg cttccgcctt
     3061 agccgactga ttcgtgagct tgaggttgtc cgttcagcca gcaacggctt cactttcatt
     3121 cgtgatttca tctacttacc atttgttgtt gtcggtcgct ggatgagcga gaaatacgct
     3181 caggtgaatg ttgttgccct tgcccttgat atgatgattg aactgccgct caaaacagta
     3241 cttcgcctaa tccgtcaatg gggttcattt atcgatgatc gaaaggatcg catttaatgg
     3301 cgagagagct caatggtctc gaactccagg ggtttattaa ggagcgtcaa cttcgtcagg
     3361 tgcgaaattt acgccagcaa tttagcatta cgccaaagct tctcattatt aaaagcccat
     3421 ccgcaagtgc ggtaatt
//
LOCUS       DATIIX010000039         2685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_426749, whole genome shotgun sequence.
ACCESSION   DATIIX010000039 DATIIX010000000
VERSION     DATIIX010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426749"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VLF64_01190"
     CDS             <1..393
                     /locus_tag="VLF64_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90591.1"
                     /translation="IDNPLAVVRTIIPNLGPSDRRVTLEPEVNRYLAASHMNALKKGS
                     AGRIDNIFALRSPWCFELSDLGDKLTYLIQGAADDQFTTPEHFIYLSDNIPNCRPIKR
                     LDMGHMGTMALELKCFRALARDAFSKGY"
     gene            981..1187
                     /locus_tag="VLF64_01195"
     CDS             981..1187
                     /locus_tag="VLF64_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90592.1"
                     /translation="MAKALCGKGWKRTLLWLSILPSLLLAIDVTDQRGLPTTGGIISA
                     IATSAVLLVGAILLGAVRLTFDES"
     gene            complement(1435..1509)
                     /locus_tag="VLF64_01200"
     tRNA            complement(1435..1509)
                     /locus_tag="VLF64_01200"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1475..1477),aa:Gln,seq:ctg)
     gene            complement(1574..2392)
                     /locus_tag="VLF64_01205"
     CDS             complement(1574..2392)
                     /locus_tag="VLF64_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90593.1"
                     /translation="MKTIQPTSTIMKVAIISAATIMGGSALIPAPANALSLDGSAKAT
                     TPNVKVDTNTDVNVTTDSTSTSTANEKARLQNTITKGDQEIARRLASLSKLTSKIDAA
                     TKLTNADKATLTAEVNATTSGLNTLKAKLDAETTMVAARADVQSIYTEYRVYALVAPK
                     VGLIKVADDQQVAEEKLQALIQKFQTRLNAAKSKGKNVDALEAVLSDMTQKVNASASI
                     SSTIQAKVIVLQPTDYNNDHAVLSGDSAQLKTAYADNIAAYQDAKQIVAGLKGL"
     gene            complement(2429..>2685)
                     /locus_tag="VLF64_01210"
     CDS             complement(2429..>2685)
                     /locus_tag="VLF64_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90594.1"
                     /translation="AIFVFAPHSNSPTASTTTGGSKAGIDSQSQTAVNAPINPTNTTD
                     TALGSDLSSVQASLTQSSSDQSTADNSINDNQQQVSIPTE"
BASE COUNT          631 a          629 c          633 g          792 t
ORIGIN      
        1 attgataacc ctttggcggt agtacgaacc atcattccta atctgggtcc ttcagatagg
       61 agagtcaccc ttgagcctga agttaatcgc taccttgcag catcacacat gaatgcgctt
      121 aaaaaaggtt cggctggaag aatcgataat atattcgccc tcaggtcacc ctggtgcttt
      181 gagctgtccg atcttggcga taagcttact taccttatcc agggagccgc ggatgaccaa
      241 ttcaccacac cagaacattt tatttattta agtgataata taccaaattg cagaccaatc
      301 aaacgattgg atatggggca tatgggaaca atggcactag aactgaaatg cttcagagcg
      361 ttggcaagag atgcatttag taaggggtat taaagaatta ttatatccac gtcattcttt
      421 ttacgaatgt tatcgttcac agctaccaac tagtacgatc aacctacgag atggtcctgg
      481 cgcaacttgg gagtgagata caaagaaact cttggagcaa cgctgccgca accataaacc
      541 ctgtttctgt gattcttgct acaagacgcg cgactactgt cccgacataa aagttgcgat
      601 tgcacgtttg cgtagcagta tgcatagaga tgtcatcagt ctcacgagtt ctaataatag
      661 gcatgagacc aaaaaagtga caaaaatgcc actttttaca tgtcaggatg tacatcaaac
      721 accttagacc attcgggtca ccttaggtga gagatggtta caagtcggag agcttcttca
      781 tgcagagagg ccatgatact tgattaacta tcaggtatat gttatagtaa aagacaatta
      841 ctctcatcag taatcgcact gccccatcat cttcggttat gacgccctgc ctacttcttc
      901 gctggtagag cgtcatgacc gaatcgatga actaaggaca aatcatgtcg gaaaactcgt
      961 tcatttgtaa cgcctggatc atggcgaagg cactctgcgg taaggggtgg aagcgtactc
     1021 tcctctggct gagtattctc ccctcactgc tgctggccat cgacgtgact gaccagcgcg
     1081 gcctccccac cacggggggc atcatctcgg ccatcgcaac ctccgcggta ctcctcgtgg
     1141 gtgccatact cctcggagcc gtgaggttga ccttcgatga gtcctgagtg agcctccgac
     1201 acccccaccc cgtgtccacg acgaagaaca ccccgtgtcc gttgtggtgc ggggtgttct
     1261 tcgtttttat agaactccta gactatttct agcgcatgtt ctagacctat atttaaacag
     1321 acaggcccac cattcgggac acacatacaa aaaagcagca tttctgctgc ttttttgcta
     1381 cctaggtata caccaaccct ttagaaccac tatggtcttg cttggtggat gccttggctg
     1441 ggatggaggg attcgaaccc ccgaatgctg gaaccagaac ccagtgcctt accacttgga
     1501 gacatcccat cagtactagg ggaaattgta tcactagtac ccgtaaaact caaggcctca
     1561 ggctatttac ggcttatagt cctttgaggc ctgcaacaat ttgctttgcg tcttggtaag
     1621 ccgctatgtt atcagcgtat gcagttttta actgtgcgct atcaccgctt aatactgcgt
     1681 ggtcattgtt ataatcggtt ggctgtagaa cgattacttt agcctgaata gtgcttgata
     1741 tgctcgccga cgcattgacc ttttgtgtca tatcgcttag tacggcttcg agagcatcca
     1801 cgtttttgcc ctttgacttc gcggcattta gacgagtttg aaacttctga attaacgctt
     1861 ggagcttctc ctctgctacc tgttgatcgt ctgccacttt tatcaggccg acttttggtg
     1921 caacaagcgc atatacgcgg tattcagtat aaatactctg tacgtctgca cgtgcagcga
     1981 ccattgttgt ttctgcatca agttttgctt ttaatgtatt gaggccggat gtcgttgcgt
     2041 tgacttcggc tgtaagcgtt gccttgtcgg cgtttgttag tttggttgct gcgtcaattt
     2101 tacttgtaag ctttgagagt gacgcaagtc ggcgtgcaat ctcttggtca cccttagtga
     2161 tggtgttttg gagtcttgct ttttcgtttg cagtacttgt gctcgtagag tccgtcgtta
     2221 catttacatc cgtgttcgtg tctaccttaa cgtttggtgt tgtcgctttt gctgaaccat
     2281 cgagcgaaag tgcattcgct ggggctggaa tgagtgcgga accacccata atcgttgccg
     2341 ctgagatgat tgcaactttc atgatagtag atgtaggttg tatggttttc atgatatatt
     2401 ccttccgtat tagtacgatc gcgttaggct attctgttgg aatgctgacc tgctgctgat
     2461 tatcgttgat agagttatct gccgtactct ggtcgctcga tgattgtgta agcgatgctt
     2521 gtacactact taagtcgcta ccaagcgcgg tatctgtggt attcgttggg ttgataggcg
     2581 catttactgc tgtctgtgat tgtgaatcta tcccagcctt gctgccccct gttgtagtac
     2641 ttgctgtagg gctgttagaa tgcggcgcaa acacgaagat tgcta
//
LOCUS       DATIIX010000040         2995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_510714, whole genome shotgun sequence.
ACCESSION   DATIIX010000040 DATIIX010000000
VERSION     DATIIX010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510714"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="VLF64_01215"
     CDS             complement(<1..1379)
                     /locus_tag="VLF64_01215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HSW90595.1"
                     /translation="MGKSHTTTRFWHRRSSEAVILVSLVVVALYGWGLISGATELNQA
                     VHVPLVAGIIGAVSLIIASVTYFITPLQQLNQAAILNYALLLATITTLVVTTNGINST
                     FLSLLVASLGFASFFGIGGVIIGALSLAAFVVNQDISSMITPSQIVAIALLGGVPLIG
                     SFIIWGRHEGESGSDETKEDRSYHELASELSQVSGKSEVVINAIGEGVLALDGQGIIQ
                     LINPAAEQLLGWSRSDSLNLSYKSILKLLNSKNQEVDSANDPVAQALNTNKAISSENF
                     SLQTQSGKSFLASITVNPVGQLGKGVIVVFRDVTVEKSDERQRAEFISTASHEMRTPV
                     ASIEGYLGLALNPATANIDDKARDFIMKAHESAQHLGRLFADLLDVSKADDSRLKNDP
                     KVVDVVPFIHDIVQGLAQKGIEKGLRILYKPMPDDDENDHDGERKLNPVFYANVDNDH
                     LREVIQNLV"
     gene            1510..2355
                     /locus_tag="VLF64_01220"
     CDS             1510..2355
                     /locus_tag="VLF64_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HSW90596.1"
                     /translation="MVQGLKTTLLMAGLTGLFMFVGYLLGGQSGMLIALGLAAISNVG
                     MYWLSDSLILKMQGAKPLGNRYPQVERIVAELAQQENMPMPKLYYVDTDIPNAFATGR
                     SPKHAAVAVTAGIMDILNDNELRAVLAHELGHVKNRDMLVSTIAATMAGAISYIAELA
                     FWFGGSQDREEGNPIAGIAAMILAPIAAVVIQMAVSRSREYLADEHSAHQLGSGKDLE
                     TALMKLENWKQHVPPIAPSPSEQVTSALMFSNMFSTAGLAGMFSTHPSTADRVKRLRE
                     IDHRN"
     gene            complement(2306..2680)
                     /locus_tag="VLF64_01225"
     CDS             complement(2306..2680)
                     /locus_tag="VLF64_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90597.1"
                     /translation="MSRPKSPLQPNELGALYPHAKTYDELQVDRVAVQKLRDSWQPKH
                     PKLLITVIIASLCIIFWGILEFILPIIPTNPIFAVPTGILLGLAWVYALIGSLHKIRK
                     LLNQIALSSGDQSRVGASRDQR"
     gene            2704..>2995
                     /locus_tag="VLF64_01230"
     CDS             2704..>2995
                     /locus_tag="VLF64_01230"
                     /inference="COORDINATES: protein motif:HMM:NF024232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HSW90598.1"
                     /translation="MKAVKTMIARWYYQMDEIFAESEAWSLFKTLAILETIGWTLLII
                     GIMFKVNHWPLADVAIAIGGSAHGMIVIAYMLVVLFAHRSMGWSVWKFTVAEL"
BASE COUNT          737 a          649 c          794 g          815 t
ORIGIN      
        1 tcgacaaggt tttgtataac ctcacggagg tggtcgttat caacattagc atagaataca
       61 gggtttaatt tgcgttcgcc gtcgtggtcg ttttcatcat catctggcat tggcttatag
      121 agaatgcgaa ggcccttttc gatgcctttt tgtgctaaac cctgtacgat atcgtggatg
      181 aatggtacga catcaacgac tttcggatca ttcttaaggc gcgaatcgtc tgccttactg
      241 acatcaagca gatcggcaaa taatcgacca agatgttggg ctgattcgtg agctttcata
      301 ataaagtcgc gagctttgtc atcgatattg gcggttgcag gattgagcgc gaggccaagg
      361 tagccttcga tactggcaac tggcgtgcgc atttcgtggc tggcggtact aatgaattcg
      421 gcacgctggc gttcatcaga tttttcgaca gtaacatcac gaaacacaac aataacgccc
      481 ttgccgagtt gtcctactgg gttaactgtg atggaggcaa ggaacgattt gcctgattgt
      541 gtttgcaggc taaagttttc gcttgagatt gctttattcg tgttgagtgc ttgggcgacc
      601 gggtcgttag cgctatctac ctcttggttt tttgaattga gcaacttaag gatggatttg
      661 taactgagat ttagcgagtc ggatctgctc caacccagca gttgttctgc cgctgggttg
      721 ataagttgaa taattccctg cccatcgagg gcgaggacac cttcgccaat ggcgttaatc
      781 accacctctg atttaccaga tacctggctg agctcactag caagttcgtg gtatgagcgg
      841 tcttctttgg tttcatcgct gccagattcc ccttcgtggc ggccccagat aataaagcta
      901 ccaattagcg gtacaccacc tagtagcgca attgccacaa tttgacttgg agttatcatg
      961 ctagagatat cttgattaac gacaaatgcg gcaagtgaca gtgcaccgat aatgacaccg
     1021 ccaataccaa aaaagctggc gaacccaagt gaagcaacta acaggctaag aaacgtggaa
     1081 ttaataccgt tggttgtgac gacaagcgtc gtgatggtag caaggagcag cgcataattt
     1141 aatatcgctg cttggttaag ctgttgcaat ggtgtaatga aatacgtaac gctggcaatg
     1201 atgagtgaga ctgcgccgat aataccagcg acgagcggta cgtgtacggc ttgattgagc
     1261 tcggtggcgc cactaataag cccccaccca tagagcgcga caaccacgag tgacactaat
     1321 attaccgctt cgctagatcg acggtgccaa aagcgcgttg ttgtgtgtga tttccccatg
     1381 ccccctaatt tgtctattac tgcttatagt tagtttacca cttgtggtgg caaaaaacag
     1441 cccttattgc agtgtatttt cgagtggtca ttaaggttgc gcaaacccct aatggggtat
     1501 actagaacta tggtacaagg gcttaagacg acactactta tggctggatt aactggattg
     1561 tttatgtttg taggctacct gctgggcgga cagagtggca tgcttattgc actaggactt
     1621 gcggcaatct caaatgtcgg catgtactgg ttgagtgatt cacttatctt aaaaatgcaa
     1681 ggtgcaaagc cgcttggtaa tcgctatcca caagttgaac ggatcgttgc cgagcttgcc
     1741 cagcaagaaa atatgccaat gccgaagctc tactatgttg atacggatat cccgaacgcg
     1801 tttgcaactg ggcgttcacc aaaacatgct gctgttgccg tgacggcagg tattatggat
     1861 attttgaatg ataacgagct gcgagcggtg cttgcccatg agcttgggca tgtaaagaac
     1921 cgcgatatgt tggtatcgac aattgctgca acaatggcgg gcgcaattag ttatattgct
     1981 gagcttgcct tctggtttgg tggtagccaa gaccgcgagg aaggcaatcc gattgctggc
     2041 atcgccgcga tgattttggc gccgattgcc gcggtggtga tccagatggc tgtatcacgt
     2101 tcgcgcgagt atcttgccga tgaacatagc gcgcatcaac tgggttcggg caaagatctt
     2161 gaaacggcgc ttatgaagct cgaaaattgg aagcaacacg tgccgccaat tgccccatcg
     2221 ccaagcgaac aggtgacctc ggcgctgatg tttagtaata tgttctcaac tgcgggtctc
     2281 gcgggtatgt ttagcactca cccatctacc gctgatcgcg tgaagcgcct acgcgagatt
     2341 gatcaccgga actaagggcg atttgattga gtagtttgcg tattttatgc aaactaccaa
     2401 tgagcgcata tacccacgca agtcccaata agatgccagt cggtacggca aagatcgggt
     2461 ttgtcggtat gattggtagg ataaattcga gaatgcccca aaatattata cagagcgacg
     2521 cgatgatcac agtgataagt aatttgggat gcttcggctg ccatgagtca cggagttttt
     2581 gcaccgccac tctatccacc tgtaactcgt cataggtttt tgcatgcgga tacagtgcgc
     2641 cgagttcgtt tggttggagt gggcttttgg gtcggctcat tatctttcaa gtatactaga
     2701 tgtatgaaag ctgtgaaaac gatgattgcc cgctggtact accaaatgga tgagatcttt
     2761 gctgagagcg aggcatggtc actatttaag acgttggcaa ttctggagac aattggctgg
     2821 acactgttga ttattggcat tatgtttaag gtgaatcatt ggccgcttgc ggatgtagca
     2881 attgctattg gcggctcggc gcatggaatg atcgtgattg cgtacatgct cgtggtgttg
     2941 tttgctcacc gtagtatggg ctggagcgtg tggaagttta cagtagcgga actga
//
LOCUS       DATIIX010000041         3734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_260371, whole genome shotgun sequence.
ACCESSION   DATIIX010000041 DATIIX010000000
VERSION     DATIIX010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260371"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..442)
                     /locus_tag="VLF64_01235"
     CDS             complement(53..442)
                     /locus_tag="VLF64_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90599.1"
                     /translation="MILLWDSSTMVARITFVRDDGMKNAYEWEAGRTLARDMLMHLRD
                     LLAEQSLTFTNITGIGVKRGPGSYTGLRIGMTVLNTLASAEHIAIVGATGDVWQDDCL
                     RRLASGEDDKMVLPEYGGDANITQPRK"
     gene            complement(439..894)
                     /gene="tsaE"
                     /locus_tag="VLF64_01240"
     CDS             complement(439..894)
                     /gene="tsaE"
                     /locus_tag="VLF64_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HSW90600.1"
                     /translation="MIIEVKSTGAMQALGAKIGDRIMGGEVIVLIGDIGAGKTTFTKG
                     LARSMGISEDVQSPTFTVSRVYETGRGVTLAHYDFYRLSDPGIMRAELMEAVHDPRAA
                     TIIEWSEIVADVLPTDTLRMIITATGEDTRIVELVPGGERSRALIEEVA"
     gene            962..2743
                     /locus_tag="VLF64_01245"
     CDS             962..2743
                     /locus_tag="VLF64_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HSW90601.1"
                     /translation="MRISDDTLRKLLIENDIASEEQLKPLKEEAIRTSQSLQDTVINS
                     HLITEAELTKLYATYADIPYIEIDPHDITPETLNRIPERIARQYEAVIFKVDEDGLLH
                     LAMDDPDDVQAISFLEKEIGTNVKIYVAPHENILQCLENYRGDVNEELNQVIDIQRED
                     GGTAQQVSEADVAEDSPIAQTVNLLLEYAIRSQASDIHIEPRENFVQIRYRIDGVLKE
                     VNQLPRNVLGALISRIKILSNLKIDERRVPQDGRFKIKIAGKQYALRVSTLPIADGEK
                     VVMRILDESNQAVTLQQLGYWGHSLEVISDAMTEPNGMILITGPTGSGKSTSLFSILT
                     MLNKPDVNISTVEDPVEYKIPGVNQTQTNVKAGMTFANGLRALLRQDPNIIMVGEIRD
                     GETANLGVQAALTGHLVFSTLHTNNAATSLPRLLDMGIEPFLIASTVKAAVGQRLVRR
                     LCMVCRQQYAPGPEEIAEVTKLFNLRGNQTFAYIHELELQAMQQQVGGKTPAGTTDQT
                     IVSMWHANPDGCEACNHAGYKGRIGIYEVLGVSIPIQKMITANATSEQIQEQAIADGM
                     TTMQTDGLVKVLRGNTTLEEVLRVTRE"
     gene            2748..>3734
                     /locus_tag="VLF64_01250"
     CDS             2748..>3734
                     /locus_tag="VLF64_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSW90602.1"
                     /translation="MPNFIYVASNKQGRSLSGIIEATDRSAALSALSNQGLTPIDIKE
                     GASKKATPSFLSTLGGGKVKSDDLVMFTRQLSAMVSAGVPILRALNSQHDHTDSKVLK
                     TVLASIIKDVQGGSALADALAKYPNTFSDVYVNMVRAGEAAGILDEILKRLALQQEKN
                     ATMRKKIKSAMTYPMVLVFITVIAFFGLMLFVIPQIGKILTDLGGPDAKLPPLTQAML
                     SISSFLLHYGLFLLPVIAGGIYTLTRWLKTMAGKRVLHTVVLRVPMIQPIIQKVAVAR
                     FARTFSALIGAGVAVLEALSVTARAVGNVVYEDALMDAAEQVKNGKTLSSVIE"
BASE COUNT          998 a          931 c          842 g          963 t
ORIGIN      
        1 ttaaacggat catagcccga aaaaaacgga ctagtttaat tgtagcacgt tcctatttgc
       61 ggggctgtgt gatattcgcg tcgccaccat attctggcaa caccatttta tcatcttcac
      121 cagatgcgag ccgacgtaga caatcgtctt gccatacatc acctgttgcg cccacaattg
      181 caatatgctc tgcagaagca agcgtattta agactgtcat tccaatacga aggcccgtgt
      241 aactacctgg gccgcgcttt acaccgatgc ccgtaatatt ggtgaatgtt agcgactgct
      301 cggcgagtag gtcgcggagg tgcataagca tatcgcgagc tagtgtgcga cccgcctccc
      361 attcataggc attcttcata ccgtcatcac gcacaaatgt gatacgtgcc accattgtgc
      421 tactatccca aagtaaaatc atgcgacctc ctcaattaac gcgcgtgatc gctcgccgcc
      481 ggggactagc tcgactatac gggtatcttc accagttgct gtaatgatca tacgaagggt
      541 atctgttggc aaaacatccg ccacgatctc gctccactca ataattgttg ccgcccttgg
      601 atcatgcact gcttccatta attctgcacg catgattcct ggatcgctca agcggtaaaa
      661 gtcgtaatgg gcaagtgtta caccacgtcc tgtttcatac acgcggctca cggtgaacgt
      721 gggactttgt acatcttcgc taatccccat tgaccgtgct aggccttttg taaaggtggt
      781 tttgccagca ccaatgtcac caatcaacac aatcacttcg ccgcccataa tccggtcgcc
      841 aattttagcc ccgagtgctt gcatagctcc agtcgacttc acctctataa tcatagagct
      901 atcataccac attttgccct agcactcacg gctcgcatcg agtacaatag gcgtaagcat
      961 tatgcgtatt tcagatgaca cactgcggaa acttctcatt gagaatgata tcgcatccga
     1021 agagcaattg aagcctctca aagaggaggc tattcgaaca tcgcaatcac tccaagacac
     1081 tgttatcaat agccacctta taactgaagc cgagctcacc aaactatatg cgacctatgc
     1141 agatataccg tacatcgaaa ttgatccgca tgatattacc cctgaaacgc tgaaccgcat
     1201 cccagagcgt attgcacgtc aatatgaggc tgttatcttt aaggttgatg aggacggact
     1261 gcttcatctc gctatggacg acccggatga tgtccaggcg attagcttcc tcgaaaaaga
     1321 gatcggcact aatgtaaaaa tttacgttgc gccacacgaa aacatcctcc agtgtcttga
     1381 aaattatcgt ggcgatgtga acgaagagct gaatcaggtt atcgatattc agcgcgaaga
     1441 tggcggtact gcgcagcaag tatctgaggc ggatgttgcc gaggactcgc caatcgccca
     1501 aactgttaac ctattgctcg aatatgcgat tcgttcgcaa gcatctgaca tccacattga
     1561 accgcgtgag aacttcgtgc agattcgtta ccgtattgac ggtgtactga aagaggttaa
     1621 ccaactacca cgtaatgtac ttggagcgtt aattagtcgt attaagattc tttcaaatct
     1681 taagattgat gaacgacgcg tgccacaaga tggtcgtttt aagattaaaa ttgctggcaa
     1741 gcaatatgcg cttcgtgtct ctaccctacc tatcgctgat ggcgaaaaag ttgttatgcg
     1801 tattcttgat gagtctaacc aggccgtcac tttgcagcaa cttggctact ggggtcattc
     1861 actggaagtc atcagcgacg cgatgaccga gcccaacggt atgatcctta tcactgggcc
     1921 aacaggtagt ggtaagtcta cctctttatt ctcaatcctt accatgctta ataaaccaga
     1981 cgtgaatatc tctaccgttg aagatcctgt cgagtacaaa attccaggcg tcaatcagac
     2041 tcagacaaat gtgaaagcag gcatgacgtt tgctaacggc cttcgtgccc tcctccgtca
     2101 agatccaaac attattatgg ttggagagat tcgcgatggc gaaaccgcca atctaggcgt
     2161 ccaggctgct ttaactggtc accttgtatt tagcacgctt cacaccaaca acgccgcaac
     2221 ctccctgcca cgtctacttg atatgggcat tgagccattc ttgattgcga gtaccgttaa
     2281 ggctgcggtc ggtcaacgcc ttgtacgccg cctttgcatg gtgtgccgcc aacaatatgc
     2341 gccaggtcct gaggagattg ccgaagtcac taagttattt aacttgcgag gcaaccaaac
     2401 attcgcctat attcatgagc tcgaattaca agcaatgcag cagcaagttg gtggtaaaac
     2461 accagccggt accactgatc aaactattgt atcaatgtgg catgcaaatc cggatgggtg
     2521 cgaagcatgc aatcacgcag gctataaagg gcgcatcggt atttacgagg tgcttggtgt
     2581 aagtattcca attcagaaga tgattaccgc taacgcaaca agtgaacaga ttcaagaaca
     2641 agccatcgct gacggcatga ccacaatgca aaccgacgga ctcgtaaagg tactccgcgg
     2701 caacaccacc ctcgaagaag tattaagagt gacccgcgaa taagcctatg ccaaatttta
     2761 tttatgtcgc ctcaaataag caaggtaggt cgctctctgg aatcattgaa gcgaccgatc
     2821 ggtctgccgc acttagtgcg ctctcaaatc agggacttac tccaattgat attaaggaag
     2881 gggcatcaaa aaaggccaca ccgtcatttt tgagtacact gggcggtggt aaagtcaaga
     2941 gcgacgacct tgtaatgttt actcgtcaat tgagcgccat ggtatctgct ggtgttccaa
     3001 tcttacgagc ccttaactcg cagcatgatc ataccgatag taaagtgcta aaaacagtat
     3061 tagctagcat tattaaagat gttcaagggg gctccgctct tgcagatgca ctcgctaagt
     3121 accccaatac attcagtgat gtttacgtta atatggtgcg tgccggcgag gcggcaggta
     3181 ttttggatga aatattaaag cgacttgcac tccagcaaga gaaaaacgca acaatgcgta
     3241 aaaagattaa aagcgccatg acttatccta tggtacttgt atttattacc gttatcgctt
     3301 tcttcggtct gatgcttttt gtcattccgc agattggtaa aatccttacc gatcttggtg
     3361 gtccagatgc aaaacttcca ccgctcacac aagctatgct tagtattagt agcttcctgc
     3421 tccattatgg gctcttcctt ttgccggtta tcgctggcgg tatatacacc cttacccgat
     3481 ggctcaaaac tatggcaggt aagcgggttc ttcacactgt cgtccttagg gtgccaatga
     3541 tacaaccaat tattcaaaaa gtagctgtgg ctcgattcgc ccggactttc tcagcactta
     3601 tcggggcggg cgttgctgtt cttgaggcgc tgagtgttac tgcgcgagcc gtaggtaatg
     3661 tcgtatacga agatgcgctc atggacgccg cagagcaggt gaagaatggt aaaactcttt
     3721 catcggttat tgaa
//
LOCUS       DATIIX010000042         8964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_399089, whole genome shotgun sequence.
ACCESSION   DATIIX010000042 DATIIX010000000
VERSION     DATIIX010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8964
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399089"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="VLF64_01255"
     CDS             complement(<1..1280)
                     /locus_tag="VLF64_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-transporting two-sector ATPase"
                     /protein_id="HSW90603.1"
                     /translation="MASKFDKLVADGNPVGEIIGIDSFMVSVKGLQPTNVHAIVRFDD
                     DTRGYVHQVLEDYVVVMKLDPSPLHIGAVCVIEGPDIMTPVGKNFIGRVVNVFGEPLD
                     GKGNIEPDQEWDVFHSAPMLYERELLDKPVETGITILDLEYSLARGQRMAMLGDSKVG
                     KTALAASVAINQKNTDLTVIYVLIAKRQHDVAELVSNLEKNDALKKAVVVVSNSFESL
                     ILTYLVPYVGAAHGEYFWHQCNMDTLVIYDDLTAHAQAYREISLIAGVSPGRDSYPGD
                     MFYTHSSLLERGGKTESNHASQTLIPIVYAPGADITAYLPTNIMSITDGQWILDGQIF
                     KDTMRPAVSTGLSVTRVGGVGQNKRQKGFADQLNRTLSGYRAAEEYAHFGTELSAQAQ
                     SDYDKGKVLFKLMNQAIGETYSFAEQQFLMSIVLG"
     gene            complement(1270..1788)
                     /locus_tag="VLF64_01260"
     CDS             complement(1270..1788)
                     /locus_tag="VLF64_01260"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90604.1"
                     /translation="MIAREDIDYKEFSLPAGVVSRRDLARLVIEAEHIDDEMTAADVR
                     TRKTNRAVETPAFTGQFTDFLTDNKLDFSESKVRTDIIKELRMLKTKAPVVHTTFAVP
                     ADIESLQTLAAWFRESVHPQVLLEIGLQPALVAGVYVRTPNHVHDMSLRSALAGKREV
                     LIKDLEALRHGK"
     gene            complement(1785..2537)
                     /locus_tag="VLF64_01265"
     CDS             complement(1785..2537)
                     /locus_tag="VLF64_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90605.1"
                     /translation="MGLFHKQTDTDDESLEEAKLKQEQFFDDYFREELRNHGRWYFDK
                     VIKENGNLFKKDLEAVIAQVNTDLKDHVTKQLDGAVATLNASLKEQVTAQLNEQFSTY
                     SKTMQEAQDQALKNMTEGSDELRQQYHELSHLLKDNIAEQNSMMHHAFEDHKSQLQAM
                     DEAQIAALKWLNQSAEKLQDQYQEINTMLERQVTEQHDRLIQAFESNMASTVEHYILE
                     ALGDQFDMKAQMPGIIKQLEDNKQAMVDDIKL"
     gene            complement(2559..3395)
                     /locus_tag="VLF64_01270"
     CDS             complement(2559..3395)
                     /locus_tag="VLF64_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90606.1"
                     /translation="MKAFRTILTLSAIIAFGFFSITPVLAADNTSVSAQGYAAPSPVD
                     IGAIVTLNKDTQSVVTAKQAQLQNMFGVAVDRNQLAFAVSNSTVQNETYVAVSGTYNV
                     LVSTQGGAIESGDYVTLSSVDGIGMKASTADKTVFGRAAASFDGKTLTLGKTELKDSS
                     GAAKTVTLGTIPVTINIERNPNVKSTKVNVPNWLERFGVQIAEKQISPIRIYLSMGIT
                     AVSLITAIVMLYAGVRNAIIAIGRNPMTKRSIFRALLEVILTSILILVIGLFAVYLLL
                     KL"
     gene            3630..5633
                     /locus_tag="VLF64_01275"
     CDS             3630..5633
                     /locus_tag="VLF64_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90607.1"
                     /translation="MTVWCHLPTIFKRYRVLQTAGLITLCVAYITTLLLGTTAHAVAG
                     VNQTLSFQGRLQTNAGAVVPDGYYNMQFKIYQDGDGTTAGDTGGTLKWTESYVNNNAN
                     AGVQVKNGFFSVELGTNTPLSSVDWDQDTLWLSMNIAGPGAGCSTFGGTGCSADGEML
                     PMKRITSSPYALNSGMLGGKTADKFLQLGLGVQTDLSTSSSIYINKTASGDLIQLQNN
                     SANIFTVGNAGDLTLGSNADKTISISTATASTAGNNLTVVAGGGGTGTGSVGGNLVLQ
                     GGSAGGTNGSGGDVAIDSGVATGSGSDGVISLGASHASAINLGSSTNNTQVTINGPTS
                     VKQATDSTSAFNVTNSHNNQVLTVNTSTSQVNVGNANTVGSALNVTGGINSSTDYSIG
                     GATVLTDSALTFTGAGSASIQGSNGLSLNGGTSSLSLSNTGVQVGSGSGAGSPTLLTL
                     DKSSSNPSSSAAYGSMYYDTSLGKVQCYQSNGWGACGDSPDSFVTLSPEYSNAVMNGA
                     GTGTITSDLCSDSLNINDGSSSQPTICGTNETYNFYDWNSTQASAQTRSIYVTYQLPS
                     NFKNFVAGSTSLMARTDGSNATTSYQIYRSHSGSALTSCGSEITASTGSQTAWQQSTA
                     SGGNDPSGCSFAAGDSILIRINLTSSNSANAYVSNLNFAFSNQ"
     gene            5679..6263
                     /locus_tag="VLF64_01280"
     CDS             5679..6263
                     /locus_tag="VLF64_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90608.1"
                     /translation="MEFKRRTRPNPYAKPVAHEATPTPVAGPKPHLRDKIPRNVKGIA
                     IGFGSLIGFVVIIAVTSQILAQRDASDPSFQTILPSGQSITSLGGWRRVTPPGEDPVF
                     AYVDTIDTVAISVSEQPLPDTFKRDTQAAVADLAKQYNATTKLKAGDTTVYVGTSAKG
                     PQSTILTKHNLLILIKSKSQVDNGSWTDYINSLN"
     gene            complement(6297..6791)
                     /locus_tag="VLF64_01285"
     CDS             complement(6297..6791)
                     /locus_tag="VLF64_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90609.1"
                     /translation="MQAQPHVVHQASGHRDTSDKKSPKKWLIVLIIAVVVLILSGIGC
                     WMWTAKSDNVDTGINNSEYQAVFTTNGQVYLGKLSPYNGTYLRLTNAFIVVSPTTGQN
                     SSDTKKPDSTDALNKTDTQLVKITSGILEPQDEMFVARSQVLFYENLKPDGKAAKLIQ
                     QNNS"
     gene            complement(6943..7329)
                     /locus_tag="VLF64_01290"
     CDS             complement(6943..7329)
                     /locus_tag="VLF64_01290"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HSW90610.1"
                     /translation="MVSFVAPTVVESIVLLLTRVILSVTFFNECYIKFKDLKGFSKRD
                     GVPLQVAGFVATAELCAALGMLSGVLAQWAGIGVVLLMMSTISLHLFKWHSPYWAAKR
                     GWEYDLIMLTLASLIAVFGPGQFALI"
     gene            complement(7383..8369)
                     /locus_tag="VLF64_01295"
     CDS             complement(7383..8369)
                     /locus_tag="VLF64_01295"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HSW90611.1"
                     /translation="MNYIGSKVSLLDFIDSVVDEFADSYNRRLVICDAFAGTGTVGFF
                     FRQQGHIVIANDLQHYSYVINKNLIENPVLDLDKLDYLNNLEPVEGFIYKNYCAGSGS
                     GRIYFSDENGKRCDAIRQAIEQMRTSGQISENEYFAYLAALIEAVDKVANTAAIYGAF
                     LKKLKRAAQKPLRLKQIPTSNEIAGRVYCGDSNLLVSDISGDVLYLDPPYNVRQYSAN
                     YHLLETIARYDNPEIRGKTGLRETASQNSKYASIRTVAAEFEDIIKNARFKVIVVSYS
                     NEGLMSEKLIREIMSKYGTYYLREREHRRFRAARNHKAILTNEHLHVLVKHN"
     gene            complement(8404..>8964)
                     /locus_tag="VLF64_01300"
     CDS             complement(8404..>8964)
                     /locus_tag="VLF64_01300"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HSW90612.1"
                     /translation="YLGIVVGLSFILLVLVFRSLLVPLKATLGFLLSVGAMFGAMVAV
                     FQWGWFGISDAPGPIVSFLPIIAIGVLFGLAMDYEFFLVSSIHEEYERTHDAVGAIID
                     GYTIGSRVVFAAAVIMVSVFAGFISNKDQVIQSMGFGLAVGIFVDAFIVRLLIVPSVM
                     SLVGRAAWWIPRRFERILPHIDIEGK"
BASE COUNT         2322 a         2193 c         2048 g         2401 t
ORIGIN      
        1 ccaagcacga tactcatcaa aaattgctgc tctgcaaaac tatacgtctc accaattgcc
       61 tggttcatca gcttaaacag taccttaccc ttatcgtagt cagactgtgc ctgagcgctc
      121 aattcagtac caaagtgcgc atactcttct gctgcgcgat agccagacaa cgtacggttc
      181 agctggtctg caaagccttt ttggcgtttg ttttgcccta cgccaccgac acgagttaca
      241 gataggccag tgctcacagc tggacgcatc gtatctttaa agatctgtcc atccaaaatc
      301 cattgaccat cagtaatgga catgatgttc gtcggcaggt acgcagtgat atcagcgcct
      361 ggtgcgtaca cgattgggat gagggtttgg cttgcgtggt ttgattcggt tttaccacca
      421 cgctcaagga ggcttgagtg ggtatagaac atgtcgcctg gatatgagtc acggcctggg
      481 ctcacaccag caatcagcga gatctcacgg taagcctgag cgtgtgccgt gaggtcgtca
      541 taaataacaa gagtatccat attgcattga tgccagaaat actcaccatg cgccgcgcca
      601 acatatggca cgaggtaggt aagaattagc gattcaaatg agttcgacac aacaaccact
      661 gcttttttga gcgcgtcgtt tttctcgaga ttgcttacaa gttctgctac gtcgtgctga
      721 cgcttagcaa tcagtacata aattacggta aggtcggtgt ttttctggtt aattgctact
      781 gatgcggcaa gtgcagtttt acctaccttg ctgtcgccga gcattgccat acgctggccg
      841 cgagcgagcg aatattcgag atcaaggatt gtaatgcccg tttcaactgg tttatcgagc
      901 aattctcgtt catagagcat tggtgcgcta tggaatacat cccattcttg atcgggctca
      961 atgttgccct tgccatcgag tggctcgcca aatacattta cgacacgacc aatgaagttt
     1021 ttacctaccg gtgtcatgat gtcaggacct tcgattacac ataccgcgcc gatatgcagt
     1081 ggtgatggat cgagtttcat gacgactacg taatcttcga ggacctgatg tacgtagcca
     1141 cgcgtgtcgt cgtcgaaccg cacgattgca tgcacattgg ttggttgcaa accttttacg
     1201 cttaccatga atgaatcgat accaataatt tccccgactg gattgccatc cgccacgagt
     1261 ttatcaaatt tacttgccat ggcgcagtgc ctccagatct tttatgagca cctcacgctt
     1321 acccgctagc gccgaacgta agctcatgtc atgcacatgg tttggcgtac gcacatatac
     1381 acccgcgaca agtgcaggct gcaaaccaat ttcgagcaat acttgcgggt gcacagattc
     1441 gcgaaaccag gctgcaagtg tttgcaggct ttcgatatct gccggtaccg caaacgttgt
     1501 atgcacaact ggggcctttg ttttgagcat gcgcagctct ttgatgatat cagtgcggac
     1561 tttgctctca ctgaaatcaa gcttgttgtc ggtaaggaag tcggtgaatt ggccagtgaa
     1621 ggctggagtc tcgactgcac gatttgtttt gcgcgtacgc acgtctgctg ccgtcatctc
     1681 gtcatcgata tgctcagcct cgatgacaag tcgtgctaaa tcgcgacgag aaacaacgcc
     1741 tgcgggcagg ctaaattctt tatagtcgat gtcttcacgc gcgatcatag cttgatgtca
     1801 tccaccatcg cttgtttatt gtcctcaagc tgcttaataa ttcctggcat ttgtgccttc
     1861 atgtcaaatt gatcacccag tgcctcaaga atgtagtgtt caacggtgct ggccatattg
     1921 ctttcaaaag cctggatgag tcgatcgtgc tgctctgtga cctgcctctc gagcatcgta
     1981 ttaatctctt gatattggtc ttggagtttt tcggcgctct ggttgagcca cttgagcgca
     2041 gcgatctgcg cctcatccat cgcctgcaac tgggatttgt ggtcttcgaa cgcatgatgc
     2101 atcatcgagt tctgctcggc gatattatct ttaagcaggt gactaagttc gtgatattgc
     2161 tgacggagtt cgtcggaacc ctcagtcata ttcttgagcg cttggtcctg cgcttcttgc
     2221 attgtcttac tgtaggtcga aaattgttcg tttagctgcg cagtgacttg ctcttttagt
     2281 gaggcgttga gcgttgccac ggcgccatcg agttgcttcg taacgtgatc tttaagatcg
     2341 gtattaacct gcgcaataac cgcttcaagg tctttcttga atagattacc attctcttta
     2401 attactttat cgaaatacca gcggccatgg ttgcggagct cttcgcggaa gtaatcgtcg
     2461 aaaaattgct cttgcttgag tttcgcctct tcgagcgatt cgtcatccgt atctgtttgt
     2521 ttatgaaata atcccatcct agcccacttc ttattccgct atagcttcag tagtaaatat
     2581 accgcaaaaa gcccaataac taaaatcaat atacttgtta gaataacctc aagtaatgcg
     2641 cggaagattg agcgcttggt cattgggttg cggccgatgg caataatcgc attgcgaacc
     2701 ccagcataga gcataacgat agcggtgata aggctcactg ctgtaatacc catactaagg
     2761 taaatacgga tagggctgat ttgtttttca gcgatttgca caccaaagcg ttcgagccag
     2821 ttcggtacgt ttactttcgt actcttaaca tttgggttac gttcgatatt gattgttacg
     2881 gggattgttc cgagcgtcac ggtcttcgct gcaccgcttg aatccttgag ttcggtctta
     2941 cccaatgtaa gtgtcttacc gtcaaatgag gctgcagcac gcccaaagac ggttttatcg
     3001 gcagtactag ccttcatccc aatgccatct accgatgata gtgttacgta gtcaccactc
     3061 tcaatagcgc caccctgcgt actgacgagt acattatatg taccagagac cgctacatat
     3121 gtttcgtttt gcacagtcga gttcgacact gcaaatgcga gctgattgcg gtcaactgcg
     3181 acgccgaaca tgttttgtaa ttgtgcctgc ttggctgtta caactgattg agtgtcttta
     3241 ttaagtgtga caattgcacc aatatctact gggctcggtg ctgcataacc ttgggcgctc
     3301 acgctggtat tatctgccgc taacactggt gtaatactaa aaaaaccaaa ggcaataatg
     3361 gcagaaagag taaggatagt acggaaagcc ttcactgttt atttcactcc ggagctctaa
     3421 tgagcttgag gttattatag cataagcaag ccattaaaaa gccctgcaaa tagtagttga
     3481 tatacccaca aggatatttt acaaatgctt gttgactcta cgacatgatg gtagaataaa
     3541 cacaagcttt gatggaccac cacacgtccg agtgaaataa atattttcta ctcaaaaaac
     3601 agagtaaaaa ataaagaaaa acattcaaaa tgacagtgtg gtgtcacctc cccaccattt
     3661 ttaaacggta tcgtgttctg caaacagcag ggctgattac cctatgcgtt gcatatatta
     3721 caacgctatt attaggcacg actgcacatg ctgtagccgg agttaatcag actttgagtt
     3781 tccagggacg cctccaaacg aacgccggtg cagttgtacc ggatggttat tacaatatgc
     3841 aatttaaaat ctaccaggat ggcgacggaa caacagccgg agacactggt gggacgctca
     3901 aatggactga aagttatgtc aacaacaatg caaatgctgg agtgcaagta aagaatgggt
     3961 tcttctctgt agagctcgga accaacaccc cactcagtag tgttgactgg gatcaagata
     4021 cattatggct ttcgatgaat atagcaggac caggtgccgg ctgttcaacg tttggcggta
     4081 ccggttgtag cgccgatggc gagatgcttc cgatgaaacg tattacctca tcgccgtatg
     4141 cacttaactc gggaatgctc ggcggcaaga cagccgataa gttcctacag cttggcctgg
     4201 gtgtacaaac tgacctaagt acgtcgagca gcatctacat taacaagact gccagtggcg
     4261 atctcattca actccagaac aattcagcta atattttcac cgtcgggaat gccggcgatt
     4321 taacgctggg cagcaatgcc gacaaaacca tttctatttc gactgcaacg gccagcacgg
     4381 ctggcaataa cttaactgtc gttgcaggcg gcggaggaac tggaaccggg agcgtgggag
     4441 gaaatctcgt gcttcagggt ggatcagccg gcggcaccaa tggaagtggt ggcgatgtgg
     4501 ccatcgacag tggtgtcgca accggctcag gtagcgacgg ggtgatttca ctcggcgcta
     4561 gccacgcctc tgctattaat cttggtagca gtacaaacaa tacacaggta acaatcaatg
     4621 gtccgacatc agttaagcag gcgaccgatt caacaagtgc cttcaatgtt accaactcgc
     4681 acaacaatca ggtactgacc gtgaatactt caacaagcca ggtaaacgtt ggtaatgcaa
     4741 atactgtcgg ctctgcactt aatgtcaccg gaggcattaa ctcaagcact gactatagca
     4801 ttggcggcgc aaccgtactg accgattcag cacttacctt cactggcgcg ggtagcgcat
     4861 caattcaagg atcaaatggc ctaagcctga acggtggaac gtcaagcttg tcactcagca
     4921 atacaggcgt acaggttggt agtggtagtg gggctggttc accaacactc cttacccttg
     4981 ataaatcaag ctcaaaccca tcaagtagtg cagcatacgg cagtatgtac tacgatacga
     5041 gccttggtaa ggtgcagtgt taccaatcaa atggttgggg cgcttgcggt gatagcccgg
     5101 acagctttgt aacgcttagc cctgagtaca gtaacgccgt gatgaacggt gctggcaccg
     5161 gtacaatcac atctgacctc tgttctgatt cgcttaacat taatgacgga agttcgagtc
     5221 agcctaccat ttgtggcacc aatgagacct ataacttcta tgattggaac tcaacgcaag
     5281 ctagtgcgca gacacgcagc atttatgtca cctaccagct accaagtaac ttcaagaact
     5341 tcgttgctgg atcgacttca ttgatggcac gcacagacgg cagcaacgca acaacctcct
     5401 atcaaatcta ccgctcacat agcggctcgg cgcttacctc ttgtggatcg gaaatcactg
     5461 cctcaacagg ctcgcaaaca gcctggcaac agtctacggc aagcggaggc aacgatccat
     5521 ctggttgtag ctttgcggca ggcgacagta tcttgattcg cattaacctg acttcaagca
     5581 acagcgcaaa tgcttacgtc agcaacctca attttgcatt cagcaatcaa tagccataac
     5641 cgtcactatg ctacactggt agtactaggt aatttattgt ggaatttaaa cggcgaactc
     5701 gaccaaaccc ttatgctaaa cctgtcgccc atgaggcgac ccctacccct gtggcagggc
     5761 cgaagccaca cttacgggat aaaattccaa gaaatgttaa aggtatcgcc attggatttg
     5821 gttcacttat tggatttgtc gttattattg ccgtgacctc acagatactc gcacaacgcg
     5881 atgcttctga cccaagcttt cagactattt tgccatcagg ccagtctatt acttcacttg
     5941 gtggctggcg ccgtgtgacc ccgccagggg aggatccagt ctttgcctat gttgacacaa
     6001 tagataccgt ggctattagt gtcagcgagc aaccacttcc cgacaccttt aagcgcgaca
     6061 cacaggctgc agtagcggat cttgcaaagc agtacaacgc aaccacaaaa ctcaaggctg
     6121 gagatactac agtatatgtt ggcacctcag caaaaggtcc ccaatcgact attcttacga
     6181 agcacaatct gcttattctc atcaagtcga agagccaagt tgataatggc tcgtggactg
     6241 actatataaa ttctttgaac taaagaaaaa ggagaggcta tctctccttt ttaattctag
     6301 ctgttgtttt gttggataag ctttgcagct ttgccgtctg gtttgagatt ttcgtagaag
     6361 agcacttggc tacgcgcgac aaacatctca tcttgtggct ctaggatacc actcgtaatc
     6421 ttaacaagct gcgtatcagt cttgttaagc gcatctgtac tgtcaggctt ctttgtatct
     6481 gaactattct ggcctgttgt aggtgataca acgatgaatg cattcgtcag gcgaaggtat
     6541 gtgccattgt atggtgaaag tttgccgagg taaacttgac cgttcgtcgt aaataccgcc
     6601 tggtactcgc tattattgat tcctgtgtcg acattgtcgg attttgcggt ccacatccag
     6661 caaccaatgc cactaagaat aagtacaacg actgcaataa taagtacaat aagccatttt
     6721 ttaggagatt tcttgtcaga agtatcacga tgtcccgacg cttgatggac aacatgtggc
     6781 tgagcttgca tcaccggctg aggctctttg ggtcggtatg cttgttcagg ccttgcagca
     6841 ggtcttccag atggctgtgg tgcactaaac ggacctgagt ccatgtatca ttctccctca
     6901 cttatgtaac tcgtttcatc atagcatatg cgcaatggag cttcaaatga gcgcgaattg
     6961 gccggggccg aataccgcaa tcaatgatgc gagtgtgagc ataattaaat cgtattccca
     7021 gccgcgcttt gccgcccagt atggactatg ccatttgaat agatgcaagc tgattgtcga
     7081 catcattaac aacactacac caatgccagc ccactgcgca agcacgccgc tgagcatacc
     7141 aagtgctgca cataattctg ccgtcgcgac aaatccggcg acttgcagcg gcacaccgtc
     7201 gcgttttgag aatcctttga gatctttgaa ttttatgtag cattcgttaa agaatgttac
     7261 agaaagtatc acgcgcgtaa gcagtaatac aatactctct actactgttg gtgcgacaaa
     7321 tgaaaccatg cttccctcca cgttagtaca ttaccatttt acaccctacc gttgcatcgc
     7381 ggctaattgt gttttactag tacgtgcagg tgctcgtttg tcaatatagc tttgtggttt
     7441 cgggcggccc tgaatctgcg atgttctctt tcgcgtaaat agtacgtgcc gtatttactc
     7501 ataatttcac gtattagctt ttccgacatt agcccttcgt tgctatagct tacaacgatt
     7561 actttaaacc gcgcgttttt aataatatct tcaaactctg ccgccacggt tcggattgat
     7621 gcatatttgc tgttttgact tgctgtttca cggaggcccg ttttaccgcg gatctcagga
     7681 ttatcgtacc gggcgattgt ctccagcaag tgataatttg ctgaatactg ccgaacgtta
     7741 taaggtgggt cgagatacaa gacatcgcca ctgatatcgc taactagcag attactgtcg
     7801 ccacagtaga ccctacctgc aatctcattg ctcgttggga tttgtttcaa tcttagaggt
     7861 ttctgggctg ctcttttaag ctttttgagg aatgcaccat atattgcagc ggtgttcgca
     7921 actttatcga cagcttcaat aagcgcagca agatacgcaa aatactcgtt ctcgctaatt
     7981 tgcccgcttg tccgcatttg ctcgatcgct tggcggattg catcgcagcg tttgccgttt
     8041 tcgtcggaga aatatatacg accactacct gatccagcgc aataattttt gtaaatgaag
     8101 ccttctactg gctcgaggtt gttaagataa tcgagcttgt caaggtctaa cacaggattt
     8161 tcaatcaagt tcttgtttat tacatagcta tagtgctgta ggtcatttgc aatcactatg
     8221 tgcccttgct gcctgaagaa gaaccccacc gtacctgttc ccgcaaaggc atcgcaaata
     8281 acaagccgcc tattatagct atcggcgaac tcatcaacaa cgctatcgat aaagtcgagt
     8341 agcgaaacct ttgacccaat ataattcacg tatactagcg tatcacgcct ggtatcagga
     8401 tatttatttg ccttcgatat caatgtgcgg tagaatgcgc tcaaaccgcc ttgggatcca
     8461 ccacgctgca cgaccaacga gtgacatcac cgatggtaca atgagcagcc gcacaatgaa
     8521 tgcgtcaaca aatataccta ctgcgaggcc gaagcccatc gactgaataa cttgatcttt
     8581 gtttgaaatg aatccggcaa acacagatac cataatcacc gctgctgcaa agacgacacg
     8641 gctaccgatt gtgtagccat cgattattgc ccccacagcg tcatgagtac gttcgtattc
     8701 ttcatgaata ctcgacacta agaagaactc ataatccatc gcgagaccaa ataacacgcc
     8761 gatcgcaata attggcagga agctcacaat tggccctggt gcatcagaga tgccgaacca
     8821 gccccactgg aataccgcta ccattgcgcc aaacattgcg ccgactgata gcaaaaatcc
     8881 aagcgttgct ttaagcggta cgagtagaga gcggaacaca agaaccagta gtataaatga
     8941 gagtcccaca acaattccta agta
//
LOCUS       DATIIX010000043         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_177881, whole genome shotgun sequence.
ACCESSION   DATIIX010000043 DATIIX010000000
VERSION     DATIIX010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177881"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VLF64_01305"
     CDS             <1..387
                     /locus_tag="VLF64_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HSW90613.1"
                     /translation="SMSEIQEAAEIITSAVSPDANIIFGATLKPDLEDELIITVIATG
                     FDSAFFEQQAAELERPGALVATPTPAENIDKAVSDIDMELNRSDGAEQFAAEPTENIW
                     NHPTEEDDDDTPAFLRRRKKKQSDES"
     gene            402..854
                     /gene="nrdR"
                     /locus_tag="VLF64_01310"
     CDS             402..854
                     /gene="nrdR"
                     /locus_tag="VLF64_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HSW90614.1"
                     /translation="MAHNYKIGDSRVLESRESADGITIRRRRETMDGKHRFTTYERIE
                     HPNLAVIKKDGTRELFDREKLANAIRRSVGKFFKSEVEVEEIINKVEERLYELGESEV
                     DSMAVGDYVLDELAAKNEVAYVRFASVFHKFKTLDDFVRILEQRKKGV"
     gene            857..1123
                     /locus_tag="VLF64_01315"
     CDS             857..1123
                     /locus_tag="VLF64_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90615.1"
                     /translation="MRVVVITKEDTDYAQTVDTFIADFERQTGRTLEVLDPDSLDGSS
                     FCRTYDIVEYPSIIALGNDGQMQNVWAGAMLPTISEVSYYATQD"
     gene            1107..1739
                     /locus_tag="VLF64_01320"
     CDS             1107..1739
                     /locus_tag="VLF64_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HSW90616.1"
                     /translation="MLRKIKDYFTHEDRKIRDNRWIFLSMLIGAICSLIASFVLSNEA
                     IALAKDPNAILSCSINIILNCAKVGLSPSASLFGFPNSFLGMMAEPVVITVAVAGLAG
                     VKFPRAFMFVAQIFYTAGLVFAYWLLGQSYFVIGALCPWCLLVTATTTFVWFAITRYN
                     IREHNLYLTKEASERAHRFIERDYDKVVLFSIIVLIVVAIIAKYGTALFA"
     gene            1967..>2167
                     /locus_tag="VLF64_01325"
     CDS             1967..>2167
                     /locus_tag="VLF64_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90617.1"
                     /translation="MTEQPQFIITELTAATIDDCNRMRLQSWLDTYVNDEAGGTREWI
                     EARNADQMSDEKRARRLERLDSS"
BASE COUNT          575 a          446 c          540 g          606 t
ORIGIN      
        1 agtatgagtg aaattcagga agctgctgag attatcacga gtgccgtgag tccagatgcc
       61 aatattattt tcggtgcaac gcttaaacct gatcttgaag atgaacttat tatcactgtt
      121 atcgcaacgg gttttgatag tgcattcttt gagcaacagg ctgccgagct cgagcgccct
      181 ggtgcgcttg tagcaacacc gacacctgct gagaatattg ataaagccgt tagcgacatc
      241 gatatggagc ttaatcgctc cgatggcgct gaacaatttg ctgccgagcc aacagagaac
      301 atttggaacc accctaccga agaggatgac gatgacacgc ctgcgttcct tcgtcgccgc
      361 aagaaaaaac aatcagacga gtcctagaaa ggtgacaacg tatggcgcat aactacaaaa
      421 tcggtgatag ccgcgttttg gagtctcgcg aatctgctga tggtattact atccgccgcc
      481 gccgcgagac aatggatggc aagcaccgtt ttacaacgta cgagcgcatc gagcacccaa
      541 atcttgcagt cattaagaaa gatggtacac gggagctttt tgaccgcgag aaacttgcca
      601 acgcaattcg tcgttcggtt gggaaattct ttaaatcaga ggtggaagta gaggaaatca
      661 tcaataaagt tgaggagcga ctctacgaac ttggcgagag cgaagttgat tctatggcgg
      721 tcggtgacta cgtacttgat gaattggctg caaaaaatga agttgcttat gtgcgctttg
      781 caagcgtatt ccacaaattc aaaacgcttg atgattttgt acgaatactt gagcagcgca
      841 agaagggtgt ataagtatgc gtgtcgtagt cattaccaaa gaagataccg attacgcaca
      901 gactgtcgat acgtttatag cggactttga gcgccaaact ggtcgcaccc tcgaagttct
      961 tgacccagat tcattagatg gtagctcatt ctgccgcacc tatgatattg ttgaatatcc
     1021 aagtattatt gcgcttggca atgatggaca gatgcagaat gtgtgggcgg gcgcgatgct
     1081 cccaacgata agtgaggtaa gttactatgc tacgcaagat taaagactat tttacccatg
     1141 aagatcggaa aatccgtgat aatcgctgga ttttccttag tatgctcatt ggggcaatct
     1201 gtagtttgat tgcttctttt gtgctttcta acgaagcaat cgctctcgct aaagatccga
     1261 acgcgattct ttcttgtagt attaatatta ttctaaactg tgcaaaagta gggcttagtc
     1321 cttcggcaag tttgttcggt ttcccgaata gtttccttgg catgatggct gagccagtag
     1381 tgattactgt agctgttgct ggccttgcag gagtaaaatt tcctcgcgca tttatgtttg
     1441 tggcacagat cttttacact gctggcttag tatttgcata ctggttgctt ggtcagagct
     1501 actttgttat tggcgcgctt tgcccctggt gcttgcttgt tacggcaact actaccttcg
     1561 tttggtttgc cattacacgc tacaatattc gtgaacataa tctttatctt acaaaggaag
     1621 cgtctgaacg ggcgcatcgt tttatcgagc gtgactacga caaagtggtt cttttctcaa
     1681 ttatcgtact gattgtggta gcgatcattg caaagtacgg tacagcattg tttgcataaa
     1741 tagtgctgtt tgtattacaa taactgtaaa cgtttgagcg gttatcagcc gcgagttctg
     1801 gaaagaacga gctctgcctt tggtggctca agtagaatcc agcgtgtaca cggcgaaaca
     1861 gtgacaataa agtgcgataa ggagttatcc cttttcgaaa ctggatggta ccgtccgatt
     1921 tatcggattc cagtagcgag aagggatttt tattttgaag aggattatga ctgagcagcc
     1981 acaatttatc attacagaac tgactgcagc tacgattgac gattgcaatc gtatgcgctt
     2041 gcaatcgtgg cttgatactt atgtgaatga tgaagctggt gggacccggg aatggataga
     2101 agcacgaaac gccgaccaga tgtcggatga gaagcgcgct agacgtcttg agcgacttga
     2161 tagctcg
//
LOCUS       DATIIX010000044         2751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_371995, whole genome shotgun sequence.
ACCESSION   DATIIX010000044 DATIIX010000000
VERSION     DATIIX010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371995"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1901)
                     /locus_tag="VLF64_01330"
     CDS             complement(183..1901)
                     /locus_tag="VLF64_01330"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HSW90618.1"
                     /translation="MARLPVPGSDTDTWGNVLNDFLSVEHNADGTLKTTGTISTKADN
                     TAVVHNTGNETIGGTKTFSTAPVVPSNSFPESAVANLTTDLNARLTFRGAWAASTAYA
                     INDLIAFSGMCYAVTTAFTSGASFSLTNLTPLANPSHVFNVQAYGAKGDGTTDDTVAI
                     TNAINAAFAAGVANGTYYAEIYFPPAVYLIAGNPTTGGATNGSAQIPLPVIDPAFTPA
                     KQKFVLVLKGAVDASALYHWQQTVPQEAGAVLRSTYNAGTGVPATGEVSVLGGPTPHF
                     GFGGSGTSSNGTSDTWNNMLVVVDGITIEVPQNGNICGFDFRGMAEANVLSASVLARS
                     TVTGAPTPPSPNWAFGLAMPQTNNNDNCNIGFFSCEGLVYGLIVYEHVQANSLRLINC
                     FDGLVIWPSSGFPHRNIVTYASIENCTQCVVFASTAVTKLDILCLDIEWTTGSGHFIV
                     NDSHNAAYGTIYVGSNGGDGGLTLEQALSASYGVNGGAHAKVINQDQGVGVVTAPTIP
                     TSTTALQNPFWRDAMVTIVGGTVTGIAVDGTSQLSTTPGTVCVPSGKTITLTYSVAPT
                     WQWTLL"
     gene            complement(2308..>2751)
                     /locus_tag="VLF64_01335"
     CDS             complement(2308..>2751)
                     /locus_tag="VLF64_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSW90619.1"
                     /translation="GFTLIEVLLVVAIIAILAGIVIIAVNPSKQLGDSRNTQRSADVT
                     TILNATYQYSIDNGSLPPAGGGAGITTAATEICATGAASCTGLIDLTPLTALGKYVVS
                     LPKDPQCATTCATNGTGYKINKDANGRLVVAAPAAENGKAISVNR"
BASE COUNT          747 a          654 c          608 g          742 t
ORIGIN      
        1 cctgcggagc taccacatac tggtatggca gaaaatgtta ttatgagtag tcttattttg
       61 ggcgcaatcg cgtatattag ctatctttat ttcaacagcc gccgcgaact tacaaatatc
      121 caatagtata tttttaccac aaacaaaatg accgcttcac aggaagcggt cattttaaat
      181 atttacagga gcgtccactg ccacgttggc gcaacagagt acgtcagcgt gatggttttg
      241 ccgctaggta cgcataccgt accaggagta gttgacaatt ggcttgttcc atcaacagca
      301 atgcctgtca cagtaccgcc aacgatagtc accatcgcat cacgccaaaa tggattttgc
      361 aacgcagttg ttgaagttgg tatagttggt gccgtgacca caccgactcc ctgatcttga
      421 tttataacct tagcatgagc accaccatta actccatatg atgcacttaa tgcctgctcg
      481 agcgttagac ctccgtcacc accattactt ccaacataaa ttgttccata agctgcgtta
      541 tgagaatcat taacaataaa atgaccggac cccgtcgtcc attcaatatc gagacagaga
      601 atatcgagct tcgttacggc cgtactagca aatacgacac actgtgtaca attctcaatt
      661 gaagcatagg tgacgatgtt acgatgcgga aagccagacg atggccaaat caccaatccg
      721 tcgaaacagt taataagtcg tagtgaatta gcttggacat gctcataaac aatcaaaccg
      781 tacacaagcc cttcacatga gaagaagccg atattacagt tgtcattgtt attcgtctgt
      841 ggcatcgcta acccaaatgc ccagtttggt gaaggaggtg tcggagcacc tgtgacggta
      901 gaacgcgcaa ggaccgaggc gcttagtaca ttggcctccg ccattcctct aaaatcaaat
      961 ccgcatatat taccattctg tggtacttca atagtgatcc catcgacgac gacgagcata
     1021 ttattccacg tgtcagacgt gccgttgctt gatgttccac ttccaccaaa accaaagtga
     1081 ggcgtaggcc cacctaagac ggagacttca ccagttgcag gcacaccagt acctgcatta
     1141 tatgttgatc gcagaacagc gccagcctct tgtggtactg tctgttgcca gtgataaagc
     1201 gcgcttgcat caacggcacc ctttagaaca agaacgaact tttgttttgc aggcgtgaat
     1261 gctggatcga taactggcaa cggaatctgt gcacttccgt ttgtagctcc tcctgttgtt
     1321 gggttaccgg caatgagata gacggctggc ggaaagtaaa tctcggcgta gtatgttcca
     1381 ttagcaacac cagctgcaaa tgcggcatta attgcatttg tgatagctac agtatcgtca
     1441 gtcgtaccgt caccttttgc accatatgcc tgcacgttaa acacatgcga agggtttgca
     1501 agtggcgtta gattagtcag actaaaactt gctccacttg tgaaggctgt cgtaacagcg
     1561 taacacatcc ctgaaaatgc gataagatca ttaattgcat acgcagtcga tgcagcccag
     1621 gcacccctaa acgtaagacg tgcattaaga tcggtcgtta aattagcgac cgcgctctca
     1681 gggaaagaat tacttggcac gactggcgcg gttgagaatg tttttgtgcc gccaattgtc
     1741 tcgttgcctg tattgtgaac aacggccgta ttgtcagcct ttgtagatat cgtacctgtt
     1801 gtcttaaggg taccgtcggc attatgctcg acgcttaaaa agtcattgag aacattcccc
     1861 caagtgtcgg tgtcactgcc agggacagga agtcgagcca tattttttat aatcccctac
     1921 aaagttagta cttattatga taggctatgc ggttatgttt agtcaatact atagtaatga
     1981 gctttagcta ttgtggcttc ccgagttaga gattgtgata ataaaaactg cactcgtcac
     2041 tgctcgaaaa gaatagtatc tttttaccct gcgagcaatt cttacattct ttttctattt
     2101 cgtcgaagtc gacatacatc tcatctcttg caaatgccaa atcacatatt tgtttcttgt
     2161 ctgcagatgc cggtgccact gtcttgttta cggacggcca ccatagaaag ttatcccaac
     2221 cagcgcggca ttttaataaa tacatattaa cggcaagatg agcgcaattt tacaagcgct
     2281 catcctatta tattatccgt caactggcta gcgattaacc gagatagctt tgccgttctc
     2341 tgccgctggc gctgctacaa ctagtcgtcc attagcgtct ttgttaatct tgtagccagt
     2401 accgttagtc gcacaagtag ttgcacactg tggatcctta ggaagcgaga caacatactt
     2461 gcctaaagcc gtaagaggtg tgagatcgat aagtcccgta caactagctg cgcccgtcgc
     2521 acagatttca gtagctgctg ttgtaatgcc agctccaccg cctgcgggtg gcaatgaacc
     2581 gttatctata gaatactggt aggttgcatt aagaatagtc gttacgtctg cagatcgctg
     2641 cgtgttacgt gaatcaccca attgcttgga agggtttact gcaatgataa cgatacctgc
     2701 gagaatcgcg atgatagcta cgacaagtaa aacttcgatg agggtgaagc c
//
LOCUS       DATIIX010000045         8791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_122113, whole genome shotgun sequence.
ACCESSION   DATIIX010000045 DATIIX010000000
VERSION     DATIIX010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 8791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8791
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122113"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2429)
                     /locus_tag="VLF64_01340"
     CDS             complement(<1..2429)
                     /locus_tag="VLF64_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90620.1"
                     /translation="MAVILPASALMAVYPQIAAADGTPAPYFAPAVLDAAKKSTERNS
                     IAMCLGYQEGASVRDSNYGSDIDPKVIASGSWFSGYTSVRQWSDYNNGDNGTGCDKVN
                     AGASLQDLGFTSGNIQAACGLAIFRQNGSDCVNGTGNMEYKGKGKQDLLNNIDKVYKG
                     TQFGKEYSAEQFYVMAMSVIMSDKACKASPIALGSTASADQKAQADATITVVGPDGVA
                     TAVLYKFNGNKGTSWSFRNDIGGGTAYVSDKCSELAGVANQNANAYAKWVSANQPGSP
                     GNPGTCEQYATLQVDGAFHANGDKKDQYSKLLTACNDGLANKTTPDYCKKYTAADENA
                     ACIYGFGTALASTPSGDTTDTPTAGDKPTCGIQGIGWLVCPAMTFMAKIADESYSFLA
                     SNFLSVNTNLITQSQSTWAKFRDIANVIFVIVLILIIYSQITGIGMTNNSLKRKLPRL
                     LAFAVLVNMSNYICLGVVDLSNFTGYASAQFFNGLTIGGGTTDGGTLASVGSGITWAG
                     AVGVVLVAGVGVALAISVPVLLAAGLAIALIVLILVGREAGIVILADIAPVAIALGLL
                     PNLEQVPKKWWKAMFALCVVFPEVGILFGATHMTAVIIANAGGSSGQDITQFVALGVS
                     AIPFFAVGPILKGSLSGLSAIGLGAVGAKLQGAANKASSKVGSQAKGSSYLGAYMGQR
                     KKIRDTNRAQGYGKGLKGRISNSRFTGKAGSHLAATGMALASKEEAEAVDHEMVAMQK
                     QSGWTEANRLDKAHDEFVEAMEGGDTTRARAAQKILLSGGNAGISKLQSGYSKLGQQM
                     DSSGYGNMMGSA"
     gene            complement(2555..3667)
                     /locus_tag="VLF64_01345"
     CDS             complement(2555..3667)
                     /locus_tag="VLF64_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90621.1"
                     /translation="MAERMNRGHNKLKPWERLGSLAAVVALGTAAIAGCASETVAAGH
                     GPDRPAASASATPGSSESSPAPSESASTSPEKTKSFEMGETVLTNSTVYNELSELGQT
                     DVEKNAQLSFMDFEKLPLEERAMVALVLSDAHSKEYLDGVKTVVQPGNYGKSMGEILS
                     TAESYIPKGVADANGKVNAAQDLYYWTLSSTPEDVTASNNGQPYFMVQFERAIAGAMA
                     STGDPKLLEDAKKIMGGSLYHGSEGVANASGPAYDVNEWGHLTERINGLRAMSETKTD
                     TLKYLGVPDAGTNRHYFVDQAGGPSNVTLGFQIYNPDTQSMEDGVNSDGAGAAAYHDV
                     VFKDKDGRSRFTWVESETTASPDGIGIGVLMPNSTK"
     gene            3961..4989
                     /locus_tag="VLF64_01350"
     CDS             3961..4989
                     /locus_tag="VLF64_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HSW90622.1"
                     /translation="MAVYKVAQDVEADDKILGPFSLRQFIYLIIVAISIALAWGLGQL
                     FIGLAIIPLPLILFFGALALPLRKDQPMEVYLGALISYYLKPHRRTWEPDGIESLIEI
                     TAPKVIEVQRTKDITETEAERRLSYLVDIADTQGWAVRHTAQPANNSMISDAYFEAQQ
                     TEDVLDVNGGVSQSLGAMINQADERRKQSMMARMQQQVAQTPVSQQPQPQLQSVYVPP
                     VTDPYASLAQRTPAPQPAQPQYNDPSVQFNPYPNAIHQSVIQPLEQESQLPAQPVVQN
                     NPAPSPPTQSQSAADVAPTTSEKAISPDIINLASNTDLSIETIQREANRIQHKADEDS
                     SEVFISLH"
     gene            5008..5934
                     /locus_tag="VLF64_01355"
     CDS             5008..5934
                     /locus_tag="VLF64_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90623.1"
                     /translation="MGGNVPPNAPGANQMPQQPMQQAPGSFNATPLPEQQFTPPQQPM
                     MQQIPQQQSAQAPQTEQPIKKRATTTQNSLLLSELRDSMVIMADGSFRAVIACKSINF
                     DLMSSREREGVEFSYQNFLNALYFPIQIFIRSQRVDIGPYLDKLEDIRRNNDNMLLNV
                     LMDDYINFIDALAQEANIMDKSFFIVVPYFPAGDMSSVVEQGKGLFGKVFGGTRTNNT
                     ITKIDKVTYEKAKDEIKNRVDAVLSGLLQVGVQSTQLNTKQLGELYYNVYNPDTAVRE
                     PLTDFNNVTSTYVRKAPSPNQSRSPMQPGGQL"
     gene            5934..7790
                     /locus_tag="VLF64_01360"
     CDS             5934..7790
                     /locus_tag="VLF64_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HSW90624.1"
                     /translation="MAKKKLDPVDIAAQQRAREQAEVEQAFLKGMTTLRDLIAPSSLE
                     IHSSHFRIGTKYGRTMYIYGYPRELYTGWLSGLINIDQVIDISMFVYPVETEVVLNNL
                     RKKVTQLEADMSINSEKGRTRDPAKEAALQDAEELRDQLQVGSERFFRYGLYVTMYAD
                     SLEELGFVQHALETLFGQQLVYTKTASSQQEQGLNSTIPQMADQLQIRRNMNTGAIST
                     SFPFTSADLTQEKGVLYGINMHNNGLVIFDRYSLENANMVVFAKSGAGKSFTVKLEAL
                     RSMMLGADILIIDPENEYQKLSDAVGGSYIRLSLSSDTRINPFDLPRVIDSDEADDAL
                     RANLVTLHGLLRLMLGGSQMAANGQIMAGLTPAEEADLDQGLIDTYARVGITSDPLTH
                     NSIPPTISDLYDTLLHMGGTGPQLAQRLRKYTSGTFAGIFSQQSNIDINNNMVVFNIR
                     DLEDELRPVAMYIVLSHIWNITRTVQRKRMLIVDEAWQLMKYDDSANFLFSLAKRARK
                     YFLGLTTITQDVEDFMGSKMGRAIVANSSMQLLLKQSSSAVDVLSDVFKLTEEERKRL
                     ANFPVGQGLFFAGQNHVHIQIIASDTETGLITTNPQALLQNGQGQNSGQGNA"
     gene            7814..>8791
                     /locus_tag="VLF64_01365"
     CDS             7814..>8791
                     /locus_tag="VLF64_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90625.1"
                     /translation="MATDDPNSKDLNPGEQHWKNRFDSAGTLSRMENSGSNNGATTET
                     NEQTNTSDDLGDDVRDRESTGSEWENNVTGKQSPQNQGGLKGFGQKAKGLLKKKGATA
                     AIIALLGGGAAVPFLAAAELPFSMIGNQDSKSMMRGLGQYDSDYTGFRIFTNKDSVSS
                     SANKIRGLRDTEIEQLKANGVDLEGAKYNSVTKKTTFTSIKFEGQTYHAGTEFKNAMR
                     SNAAFRNSMLFTRGSYWKSSKSAFAAKVKSLYKIDPNPDISGKDDKEKSQKLMTESIE
                     GTDSHTSGSGTNEQKDKNGIDPNASEKEEATKLAGDLQGKITDEQAAIAK"
BASE COUNT         2581 a         2175 c         1882 g         2153 t
ORIGIN      
        1 gcactcccca tcatatttcc ataaccactt gagtccattt gttgtccaag tttactgtaa
       61 cccgactgta gtttactaat gccagcgtta ccgccgctca aaagaatctt ctgcgcagcc
      121 cttgcacgtg ttgtatcccc accttccatc gcttcaacaa attcatcatg agccttgtct
      181 aaacgattcg cctcggtcca gccactctgt ttttgcatag caaccatttc gtgatcaacc
      241 gcctcagcct cttctttaga tgcaagcgcc atgcctgtag ccgccagatg cgaaccagcc
      301 ttaccggtga agcgagaatt actaatgcgg ccctttaacc ccttaccgta accttgcgct
      361 cgattagtat ccctaatttt cttacgttgg cccatatatg caccaagata agatgaaccc
      421 tttgcctgtg agccaacttt gctagaagct ttattcgccg caccttgaag cttcgcgcca
      481 actgcaccca atccgattgc actcaatccg ctaagagacc ccttaagaat aggtcccacg
      541 gcaaagaaag ggatggccga aacaccaagt gctacaaatt gagtaatatc ttggccagag
      601 ctaccaccag catttgcaat aataactgcc gtcatatgag tcgcgccaaa tagtataccg
      661 acctcaggaa agaccacaca tagagcaaac atcgccttcc accacttttt gggtacttgc
      721 tcaagatttg ggagcaaacc aagggcaata gcgaccggag cgatatcagc gagaatgaca
      781 atccctgctt ctcgtccaac taatattaat acgataagag cgatggcaag gcctgccgca
      841 agaagtacag gcacactaat agccaaagca acaccgactc cagcaacaag aacaacaccg
      901 acagccccag cccacgtaat tccagaacct accgaagcaa gggtgccacc gtccgtagtt
      961 ccaccaccga ttgtaaggcc attaaagaac tgtgcggaag catatccagt aaagttacta
     1021 agatcaacca caccgaggca gatatagttc gacatattca ccaaaactgc aaatgcaagt
     1081 agcctaggta atttacgctt aagagagtta ttggtcatac caataccggt aatctgtgaa
     1141 tagattatca ggataagtac tatcacaaat ataacattag ctatatcgcg aaatttagcc
     1201 catgttgatt gcgactgtgt tattagatta gtattaacac ttaagaaatt cgaagccaga
     1261 aaagaatagg attcgtcggc aatctttgcc ataaaggtca tagcagggca cacgagccaa
     1321 ccaataccct gtataccaca cgttggctta tcgcccgccg taggtgtatc agtggtatcg
     1381 cccgatggcg tactcgcaag tgcagtgcca aaaccgtaga tacatgcagc attctcatct
     1441 gcggcagtat actttttaca gtaatcaggg gtcgttttat tagcaagccc atcgttgcaa
     1501 gcagtaagga gtttactgta ctgatctttc ttgtcgccgt tagcgtggaa agcaccatca
     1561 acctgaagag tcgcgtactg ctcacacgta cctgggtttc caggggaacc tggctggtta
     1621 gcgcttaccc acttggcata ggcgttagcg ttttggtttg caacaccagc aagttcacta
     1681 catttatctg agacataagc agttccacca cctatatcat ttctaaagga ccaactcgtc
     1741 cccttattgc cattaaactt ataaagtact gccgtagcca caccatctgg acctacgact
     1801 gtaatggttg catcggcctg cgccttctgg tcagcgctag ctgtcgatcc aagagcaatt
     1861 ggtgaagcct tgcatgcctt atcactcata ataacgctca tggccattac gtagaattgc
     1921 tcagcactat actccttacc gaactgagtt cctttgtaga ctttgtctat attgttaagg
     1981 agatcctgct tgcctttgcc tttatattcc atgtttcccg tgccgttaac acagtcgcta
     2041 ccattttgac gaaaaatagc tagcccgcaa gcagcttgaa tgttgccact cgtaaatcca
     2101 aggtcttgca aggatgcgcc tgcattaact ttatcgcaac ctgtcccatt atcaccattg
     2161 ttatagtcgg accattgacg caccgacgta tagcctgaga accaactacc acttgcgatg
     2221 acctttggat caatatcaga accgtaatta ctatctctaa cggaagcccc ctcttggtag
     2281 cccaagcaca tagcaattga gttacgctcg gtactttttt tagcggcatc aagcacggca
     2341 ggagcaaaat acggagcagg ggtgccatcg gcggcagcga tttgtgggta gacagccatc
     2401 aaagcagacg ctggtaagat caccgccatt ataagaagta gcacccgcct atacatacta
     2461 catattaaag cgtatgcggt aacaaaaagc tagtgctata taaaaacacc actcgagaaa
     2521 cgaatggtgt tcttatatac atatattatg ctaactattt agtagagttt ggcatgagca
     2581 caccgatgcc aataccatca ggacttgcgg tcgtctcaga ctcaacccaa gtaaaccgtg
     2641 aacgcccgtc cttatcctta aatacaacat cgtggtatgc cgcagcccca gcaccatcac
     2701 tattaacacc atcctccatc gactgggtat caggattgta aatctgaaac ccaagagtca
     2761 catttgaagg gccgcctgct tgatctacaa agtaatgacg attagtacca gcatctggaa
     2821 caccaagata cttaagagta tcagttttag tctcactcat agcacgaaga ccattgatac
     2881 gttcagtgag atggccccat tcatttacgt cgtaagcagg accgctagcg ttagcgacgc
     2941 cttcactacc atgataaaga gaaccaccca taattttttt tgcatcttca agcaatttag
     3001 gatcgcctgt actcgccatt gcgcctgcaa tagcacgctc gaactgcacc ataaagtacg
     3061 gctggccatt atttgaagca gtcacgtctt ctggagtaga actaagcgtc caataatata
     3121 aatcttgtgc agcattaact tttccattcg catcagctac acctttaggt atgtaactct
     3181 ctgcggtact aagtatttca cccattgatt taccgtagtt accaggttgt acgacagtct
     3241 taacaccatc aagatattct ttactatgcg catcagataa aacaagcgct accatagcgc
     3301 gctcttctag agggagtttt tcaaaatcca taaaacttag ttgtgcattt ttctcaacat
     3361 ccgtctggcc aagctcactt aactcgttgt atactgttga gttggtaagt acagtttcgc
     3421 ccatttcaaa agacttggtc ttctctggtg aagtgcttgc tgattcgctt ggcgctgggc
     3481 tactttcact tgaacctgga gtagccgacg ccgaagcagc ggggcgatcg ggaccgtgac
     3541 ctgcagccac tgtctcgcta gcgcaacctg caattgccgc tgtaccaaga gcaacaactg
     3601 cggcaagact gcccaatcgc tcccatggct ttaatttgtt gtggcctcga ttcattcgtt
     3661 cagccataag tattggttct cctttgcagt gaataaaaac tgcgtttaag tttaaccgta
     3721 atggacagtt ctgttatagc aaattttagc ctaattgtca ataccaccct tgtcacaaga
     3781 cacgcgtcaa gcacagatag gtagctacta gtcaaccatt gccttaacgt actgtgcgag
     3841 caagtataca ccttatcatc tcttaaagct agtctaatgt tgtagtactg ggctaatctg
     3901 gcctttactg gagcaaaaca gccgttattt ccactgagct aatgctatac ttaatatagt
     3961 atggcagtct ataaggtggc gcaggatgtt gaggcggatg ataagattct tggccccttt
     4021 agcttgcgcc aatttatcta tttaattatt gttgcgattt caatcgcttt agcctgggga
     4081 ctcggtcaac tctttatcgg gctcgcaatt attccattgc cactcattct attctttggt
     4141 gcccttgcac taccgctacg caaagaccag ccaatggagg tatatttagg tgcattgata
     4201 tcatattatc taaagccgca tcgtcgtacc tgggagcccg atggcattga gtcactcatt
     4261 gagattaccg ctccaaaagt aatagaagtt caacgaacca aggatattac tgaaactgaa
     4321 gccgagagac gattatcgta tctcgttgat attgccgata ctcaagggtg ggcagtacgc
     4381 catacagcac aaccagctaa taactcgatg atctctgatg catattttga agcccagcag
     4441 acagaggatg tgttggatgt aaatggcggg gtttcacaat cactcggtgc gatgatcaat
     4501 caagcagatg aacgccgcaa gcaatcaatg atggcgcgca tgcagcagca ggtggcacaa
     4561 acgccagttt cacagcaacc acagccacaa cttcaatctg tgtatgtgcc gccagtcacc
     4621 gatccatatg cttcattggc gcagcgaact ccagccccgc aacccgcaca gccacaatac
     4681 aatgacccaa gcgttcaatt caacccatac ccaaacgcaa tccatcaatc ggtgatccag
     4741 ccacttgaac aagagtccca attgccagcc caacccgtag ttcagaacaa cccagcaccg
     4801 tcgcccccta cacaatcaca gtctgcagcc gatgtggctc caactactag cgaaaaggcc
     4861 atctcacctg atataatcaa tcttgcaagt aacactgatt tatctatcga aacgatccag
     4921 cgtgaggcta atcgaattca gcataaggct gacgaggata gcagtgaggt gttcatttca
     4981 cttcactagg tcgcagtaga ggtaataatg ggagggaatg tgccgccaaa cgcgccaggt
     5041 gcaaatcaga tgccacagca accgatgcaa caagcaccgg gatcttttaa cgcaacccct
     5101 ctgcccgaac agcaatttac accaccacag caaccgatga tgcagcaaat accacagcaa
     5161 cagtctgcac aagcccctca aaccgaacaa cccataaaaa agagggcgac aaccactcag
     5221 aattcactat tactatctga gcttcgtgac agtatggtga ttatggccga tggcagtttt
     5281 agggcggtta ttgcatgtaa atcgattaac ttcgacctta tgagttctcg cgagcgtgaa
     5341 ggcgtcgaat tcagctacca aaactttctc aacgcattgt atttcccgat ccaaatcttc
     5401 attcgctcgc aacgcgttga tattggaccg tacctcgata aactcgagga tattcgtcgt
     5461 aataatgaca acatgctcct taatgtcttg atggatgatt atattaactt catcgatgcg
     5521 cttgctcaag aagcaaacat tatggataaa agcttcttta ttgttgtccc atacttccca
     5581 gcaggggaca tgtcaagtgt agtcgaacaa ggcaaaggtc ttttcggcaa agtatttggt
     5641 ggcacaagaa ctaataatac gataactaaa attgacaaag tcacgtatga aaaagctaag
     5701 gatgagatca aaaaccgtgt cgatgctgta ttaagcgggc tgctgcaagt gggcgtacaa
     5761 tccacgcagc ttaacaccaa gcagcttggt gagctatatt acaatgtcta caaccctgac
     5821 acggcagtac gtgagccgtt aacagacttc aataatgtaa cgtcaaccta tgtccgcaaa
     5881 gcgccatcac caaatcaatc gcgatcacct atgcaacccg gagggcaact gtagtggcta
     5941 agaaaaaact agatccagtc gacattgccg cccagcaacg tgcgcgtgag caagcagagg
     6001 tggaacaagc cttcctgaag ggaatgacca cactacgcga cctaattgca ccaagtagtc
     6061 tcgagattca ctcaagtcac ttccgtattg gcaccaagta cggccgtacg atgtacattt
     6121 atggctatcc gcgcgaacta tacaccggat ggctatctgg gctcattaat atcgatcaag
     6181 taatcgatat tagcatgttt gtctacccag ttgaaaccga agttgtactt aataacctcc
     6241 gcaagaaggt gacgcaactc gaagccgata tgtcaataaa ttctgaaaag ggacgtaccc
     6301 gcgacccagc aaaagaggcc gcgttgcaag atgccgaaga gcttcgtgac cagctacaag
     6361 ttgggtctga gcgtttcttc cgttatggcc tgtatgtaac aatgtatgct gacagtcttg
     6421 aagagctcgg cttcgtgcaa cacgcgcttg agacactctt tggacagcaa ctagtgtata
     6481 caaagactgc ttcgagtcaa caagagcaag ggcttaatag taccattccg caaatggccg
     6541 atcagttgca aattcgccgc aatatgaaca ctggtgcaat tagcacaagt ttcccgttca
     6601 caagtgccga cctcactcaa gaaaaaggcg tgctctacgg tattaatatg cataataatg
     6661 gccttgtaat cttcgaccgc tacagcctag agaacgccaa tatggtggta tttgcaaagt
     6721 ctggtgccgg taaatcgttt accgtgaagc tcgaagcact gcgtagtatg atgctcggtg
     6781 cagatatatt aattattgac cctgaaaatg aatatcaaaa gctttctgat gcggtaggcg
     6841 ggagctatat tcgccttagt cttagtagtg atacacgcat caatccattc gatttaccgc
     6901 gcgttattga tagcgatgaa gctgatgatg ctctgcgcgc taaccttgtg acacttcacg
     6961 gcttgttacg cttaatgctg ggtggttcac aaatggcagc aaacggacag ataatggccg
     7021 gtcttacacc tgctgaagaa gccgatcttg accaaggcct tatcgacaca tacgcacgtg
     7081 ttggtattac cagcgacccg cttacacata actctatccc accaacaatt agcgatttat
     7141 atgacacact cctccatatg ggcggcactg ggccacaact tgcacaacgt cttcgcaagt
     7201 acacaagcgg cacctttgcc ggcatcttta gtcaacaaag taacattgat atcaacaaca
     7261 acatggtggt gttcaatatc cgcgaccttg aggatgaact ccgcccggta gcaatgtaca
     7321 ttgtcctaag ccacatctgg aatattaccc gcaccgttca acgcaaacgt atgctcatcg
     7381 ttgatgaggc atggcaacta atgaagtatg acgattctgc aaacttcctc tttagtcttg
     7441 caaaacgcgc ccgtaaatac ttccttgggc tcactacgat cactcaggat gtagaagact
     7501 ttatgggcag caaaatggga cgtgcaatcg ttgcaaactc tagtatgcag ctgctactaa
     7561 aacaatcttc aagtgctgtc gatgtgctgt cggatgtatt caaacttact gaagaagagc
     7621 gtaaacgtct tgccaacttc ccagtgggac aggggttgtt ctttgctggg cagaatcatg
     7681 tgcatattca aatcattgca agtgataccg aaacagggct tatcactaca aatccacagg
     7741 ccctactgca aaatgggcag ggtcagaata gtggacaagg gaatgcatag aaccacgaaa
     7801 gtatacatga gatatggcga ctgacgatcc aaattcaaaa gacctaaacc caggtgagca
     7861 acattggaag aatagattcg actcagcggg tacgctgagt cgaatggaaa attccggctc
     7921 taacaacggt gcgactactg aaacgaacga acaaactaac acatcggatg acttaggcga
     7981 tgacgtgcgt gatcgtgaaa gtactggaag tgaatgggaa aataatgtca caggtaaaca
     8041 gtcgccgcaa aatcaaggtg gtctaaaagg gtttggacaa aaagcaaagg gactcctcaa
     8101 gaaaaagggt gcgaccgccg caattattgc gttgttgggt gggggcgcag ctgtcccatt
     8161 cctcgccgcc gcagaattac catttagtat gattggcaac caagacagca agagtatgat
     8221 gcgcggttta ggacaatacg acagtgatta taccggtttc cgtatcttta cgaataaaga
     8281 tagtgtgtca tcatcagcca ataagatcag gggtctacgc gataccgaaa tcgaacagct
     8341 aaaggcaaac ggcgttgatc ttgagggtgc taaatacaac tcagttacaa agaagacaac
     8401 ttttacgagt ataaaattcg aggggcagac atatcatgca ggcaccgaat tcaaaaatgc
     8461 tatgcggtca aatgccgcgt ttcgtaactc tatgctgttt acaagaggtt cgtactggaa
     8521 atcatcaaaa agtgcgtttg ccgccaaagt aaaaagctta tacaagatag atccaaatcc
     8581 agatatatca gggaaagacg ataaagagaa atctcaaaaa ctgatgactg aatcgatcga
     8641 aggaacggac agtcatactt cagggtcagg gacaaacgaa cagaaagata aaaacgggat
     8701 tgatccaaac gcaagcgaga aggaagaggc cacaaaactt gcaggtgatc ttcaaggaaa
     8761 aattacagac gagcaggccg caattgcaaa a
//
LOCUS       DATIIX010000046         5229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_537869, whole genome shotgun sequence.
ACCESSION   DATIIX010000046 DATIIX010000000
VERSION     DATIIX010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537869"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1089)
                     /gene="mnmA"
                     /locus_tag="VLF64_01370"
     CDS             complement(58..1089)
                     /gene="mnmA"
                     /locus_tag="VLF64_01370"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HSW90626.1"
                     /translation="MTRVYVGMSGGVDSSVTAALLVEQGHEVTGVYMKNWSQDLPGMK
                     CPWAEDLADAKRVAVQLGIDFKVFDFENEYRHKVVDYMIDEYKHGRTPNPDIMCNQEI
                     KFKLFLETALEDGADMIATGHYARVKEGKLYRAKDEGKDQTYFLYRVTTEALSKTLFP
                     LGDYLKQEVREMAEQRNLWTAQKKESMGVCFVGNVGMREFLSQYVTTRPGDIIEKETV
                     KKVGNHDGAIYYTIGQRHGLDLGGGLPYYVVGKDMDKNEIYVSRNLQDTELVRNEITL
                     TNVHWITDAPNGEIEARIRHRGQLISARLDGDRLQLERPERAITPGQSVVVYRGEECL
                     GGGIVSPTN"
     gene            complement(1086..1700)
                     /locus_tag="VLF64_01375"
     CDS             complement(1086..1700)
                     /locus_tag="VLF64_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HSW90627.1"
                     /translation="MKFIKIIVVVLVIFFGIVWTISSYLSVDDLKHCDAAPSSKKGCE
                     AADAIVAISGGDTAARTNEAIKLYKNGWGNLLIFSGAAADKSGPSNARVMEEQAIAAG
                     VDPNSIIIDETSETTAQNATETSNIFKESNITSAILVTSAYHERRAMLEFDRRATGVT
                     LRGHPVAADNQWGPMWWSTPTGWALAIPETVNSLILATGGATRQ"
     gene            complement(1697..2851)
                     /locus_tag="VLF64_01380"
     CDS             complement(1697..2851)
                     /locus_tag="VLF64_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HSW90628.1"
                     /translation="MNDNLIYLDHAAGTPLDARVLEAMQPYFSELFFNPSSPYAPAIQ
                     VRRDYEQAKSRIAVCIGGKADDLVMTAGATESINIIFGSVDGHVVTSAIEHRAVLEAA
                     KMHDHTFVMPDEKGRIAASDVAMAIRPETQLVSIHLANNELGTVQPLRDIAEVVRAER
                     QRRALAGDLTPIYFHSDASQGLGQIDVHVARLGVDALTLNAGKIYGPKQVGCLWTDRF
                     VRLRPLVRGGGQERGIRSGTENVAGTIGFATAIELATQHARGEANRLAKLRDSLESEL
                     TMAFPSAVLTGVRKKRLPGHLHISFPGLDAERLIFLLEMQGVLVATGSACAANKGTQS
                     HVLTAINLAPEIADGSLRITLGRLTDESKITRAAAILKAVIEGEAKRIGK"
     gene            complement(2857..3294)
                     /locus_tag="VLF64_01385"
     CDS             complement(2857..3294)
                     /locus_tag="VLF64_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90629.1"
                     /translation="MSVLKKVVALLIGDDNSSSVNWLKMPKKRALKKLTERELLTLES
                     EVGAKLFGPIPKGHRREFFCLDERTWIWHEEWLDEKRKLTTSTVRYEINEHGVLKVQE
                     GARYAYIDGEELQNFGIAIRMYYEQVAREVYSRDPATGESLVQ"
     gene            complement(3304..3696)
                     /locus_tag="VLF64_01390"
     CDS             complement(3304..3696)
                     /locus_tag="VLF64_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90630.1"
                     /translation="MAEQQNTSMYHVPASQEAVVVHSPEAPLTEVELAYQQAMESALD
                     AHEADPADVDRRRIEGLLGESTIAATEIIAPSPEVSFNPRDTAHLVACGQRVIRMRDI
                     ERGEGTPTVGEAIASTQDLNKGDVTHAA"
     gene            complement(3696..4454)
                     /locus_tag="VLF64_01395"
     CDS             complement(3696..4454)
                     /locus_tag="VLF64_01395"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HSW90631.1"
                     /translation="MIIDTHCHIHDSEFYLENREEVYRESREAGVMMICVSTNIRSSR
                     EAIEFCGTHAACYPIIGIHPHDTKDNDTSEIRALVEANREQIIGIGEIGLDYFYSHSD
                     RETQIRALCEQLQIAQDFNLPVSFHVREAFEDFWPIFDEFPGTRGVLHSFTDNQTNLD
                     EAIKRGLLIGVNGISTFTKEPLQKRLFTDVALENVLLETDAPFLTPTPFRGKMNLPAY
                     VTLVARHQAELKSVSPDDVIRATTTNAQRVFGIQ"
     gene            complement(4457..>5229)
                     /locus_tag="VLF64_01400"
     CDS             complement(4457..>5229)
                     /locus_tag="VLF64_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HSW90632.1"
                     /translation="PDRPEEWQEYWPADVQVVGKDILRFHAGIWPAMLLGLGISLPKV
                     LLVHGFVTVGGAKMSKSLGNGVGPMEVINSYGTDAFRYYFSRHVPTQEDGDFTWEKFE
                     NSYNNELGNDLGNLVQRVASMIQRYQAGVIGDMPKAEHDMGPYREAMAEFEFNKALDI
                     AWNTVRALNQYLENVKPWIIAKQLGKDPDAESHLAEVLAHSASTLSQIADFITPFMPS
                     TAEKIHQIFNSGVVPNTLEPLFPKIYLHTQDPRAPKAE"
BASE COUNT         1256 a         1346 c         1194 g         1433 t
ORIGIN      
        1 aggggtaatt ataccatagg catacccatt gtctcgagct tcgcctgatg gcattatcta
       61 gtttgttggc gaaacgatgc caccaccgag acattcctcg ccacgataca cgacgacaga
      121 ttggccgggg gtgattgcgc gttctggtcg ttcaagttgc agcctatcgc cgtccagccg
      181 agctgagatt agctgaccgc gatgacgaat gcgcgcctca atctcgccat ttggcgcatc
      241 ggtaatccag tggacatttg taagtgtaat ctcgttacgt acaagctcgg tatcttgcag
      301 gttgcgtgaa acgtagatct cgtttttgtc catatctttg ccgacgacgt aatacggcaa
      361 accaccgcca agatcaagac cgtggcgctg accaattgtg tagtagattg caccatcatg
      421 attgccgact tttttaaccg tttctttttc gataatatcg ccaggacgag ttgtgacata
      481 ttgacttaag aattcacgca taccaacatt ccccacgaaa cagacaccca tgctttcttt
      541 tttctgtgct gtccagagat tccgctgctc ggccatttca cggacttctt gcttgagata
      601 atcacctagt gggaatagcg tctttgagag cgcctctgtg gtaacgcgat acaaaaagta
      661 tgtctggtct ttaccttcgt ctttagctcg gtacaattta ccctctttta cacgggcata
      721 gtgacctgtt gcgatcatat ccgcgccatc ctcaagcgca gtctcaagga aaagtttgaa
      781 cttaatctct tgattgcaca taatatctgg gtttggcgta cgaccatgct tgtactcatc
      841 aatcatatag tcaacaactt tgtgacggta ttcgttctcg aaatcgaaaa ccttaaagtc
      901 aatgccaagt tgcaccgcaa ctcgctttgc atcggccaaa tcttcagccc aagggcactt
      961 catgcccggc aaatcttggc tccagttttt catatacaca cctgttactt cgtggccttg
     1021 ctcgacaagc agcgccgctg taacactgct atcaacccca ccactcattc caacatatac
     1081 acgtgtcatt gcctggtggc tcctccagtt gcgagtatca atgagtttac agtttcaggg
     1141 atggcaagcg cccaacctgt tggcgttgac caccacattg gaccccattg gttatctgcg
     1201 gcgacaggat ggccacgtaa cgtgacgcca gttgcgcgcc tatcgaattc aagcatcgca
     1261 cgccgctcgt gatacgcgct cgttactaga attgccgagg taatattgct ttctttgaaa
     1321 atattcgacg tttcggtcgc attttgcgct gttgtttcgc ttgtttcatc tatgataata
     1381 ctatttggat caacacctgc tgcgattgcc tgctcttcca tcactctagc attgcttgga
     1441 ccggatttat cagcagcggc gccagagaaa attaggaggt tgccccaacc gttcttatac
     1501 agtttaatcg cttcatttgt gcgcgccgca gtatcaccac cactaattgc tacgattgca
     1561 tcagccgctt cgcagccctt cttcgatgat ggtgctgcgt cgcagtgttt gagatcatca
     1621 acactgagat atgagctaat tgtccataca attccaaaga atatcaccaa gaccactacg
     1681 ataatcttta tgaatttcat ttgcctatcc gctttgcctc accctcaatt actgctttta
     1741 gtatagcagc agcacgagtg atcttcgatt cgtcagtcaa gcgacctaag gtaatgcgca
     1801 gactaccgtc cgcaatctct ggcgcaagat taatggctgt taaaacatgg ctttgagtcc
     1861 ccttatttgc cgcacatgca ctgccagttg caacaagtac gccttgcatt tcgagcaaaa
     1921 agatcaaccg ctcggcatca agcccaggaa atgaaatatg taagtggccc ggtaatcgct
     1981 ttttgcgcac accagtaagg actgcgctag gaaacgccat agtaagttca gactccagac
     2041 tatcgcgcag ttttgcaagt cgatttgctt cgccacgagc atgctgcgtg gcaagttcaa
     2101 ttgccgtcgc gaagccgatt gtacctgcga cgttctcagt accactgcgt atgccgcgct
     2161 cttggccgcc tccacgaacc agcggacgca agcgcacaaa tcgatccgtc cacagacaac
     2221 cgacttgctt tgggccgtaa attttccccg cgttgagcgt tagtgcgtca acaccaagac
     2281 gtgctacatg tacatcaatc tggccaagtc cctgagaggc atcactatgg aaatagatag
     2341 gtgtcaaatc accagcaaga gcacgtcgct gacgctccgc ccgcactact tcggcaatat
     2401 cacggagcgg ttgaacggta ccaagctcgt tattggcaag atgaatactt acgagctgag
     2461 tttcagggcg aatggccatt gcgacatcac ttgccgcaat tcgacctttt tcatctggca
     2521 taacaaacgt atggtcatgc atttttgctg cttcaagtac cgcccggtgc tcaattgcgc
     2581 ttgttacgac atgcccgtca acactaccaa atatgatatt aatagattct gtggctccgg
     2641 ccgtcatcac taaatcatca gctttcccac caatgcatac ggcgatacgt gattttgctt
     2701 gctcgtaatc acgacgaacc tgaatcgccg gtgcgtatgg gcttgatgga ttaaaaaaga
     2761 gttcactaaa gtacggctgc atggcttcaa gcacccgagc gtccagcggc gtgcccgctg
     2821 cgtggtcaag atagattaga ttatcgttca ctgtaactat tgtaccaggc tctcaccagt
     2881 tgcagggtca cgcgaataga cttcacgggc aacttgctca taatacatac gaatcgcaat
     2941 accaaagttc tgcaattctt cgccatcgat ataggcataa cgagcgccct cttgcacctt
     3001 taacacacca tgttcattaa tttcgtatcg cactgtgctc gtggtgagtt tacgcttttc
     3061 atcaagccat tcttcatgcc aaatccaggt gcgttcatca agacagaaga actcacgacg
     3121 gtggcccttg ggaattgggc caaatagttt tgcccccact tcgctttcaa gtgtgagaag
     3181 ttcgcgctct gtgagctttt ttagtgcacg cttttttggc atttttagcc aattaacaga
     3241 gctactattg tcgtctccga taagaagagc tacaactttt ttgaggacgc tcatatctaa
     3301 tccttatgcg gcgtgcgtaa catcgccctt gttaagatct tgcgtactag caatagcttc
     3361 acccacagtt ggcgtgccct cgcccctttc gatatctcgc atgcgaatca cccgttgacc
     3421 acaggcgacg agatgggctg tgtcgcgcgg attaaagctt acttcaggtg acggtgcaat
     3481 gatttcggtc gcagcaatcg ttgattcgcc aagtaggcct tcgatacgac ggcgatccac
     3541 atctgctgga tcggcttcat gtgcatcaag ggcgctttcc atagcctgct ggtaggcaag
     3601 ttcgacttcg gtcagaggtg cttcggggct atggacaaca acagcctctt gtgaggctgg
     3661 aacgtgatac atactcgtat tttgctgttc tgccattact gtatcccaaa aactctctga
     3721 gcgtttgtgg tggtagctcg tataacgtca tctggtgaaa ctgatttcag ctctgcttga
     3781 tgccttgcga cgagtgtcac atacgctggt aaattcattt taccacgaaa tggcgtagga
     3841 gtcaagaatg gtgcgtccgt ctctaagaga acattctcaa gtgccacatc tgtaaaaagc
     3901 cgtttctgta agggttcctt cgtgaaggta ctgatgccgt ttacgccaat taatagacca
     3961 cgtttaattg cttcgtcaag atttgtctga ttatcggtaa aactatgtag cacacccctt
     4021 gtgccaggaa actcatcaaa aatcggccaa aaatcttcaa acgcctcgcg cacatgaaag
     4081 cttactggca ggttaaagtc ctgcgcgatt tgcaactgct cgcagagcgc acgaatctgt
     4141 gtctcacggt cgctatggct ataaaagtaa tcgagcccaa tttcaccaat accgataatc
     4201 tgctcgcggt tcgcttcaac aagtgcacgg atttcactag tatcgttatc ttttgtatcg
     4261 tgcgggtgaa tcccaataat tggatagcag gccgcgtggg taccgcaaaa ttctatggcc
     4321 tcacgcgagg accgtatgtt cgtgctgacg catatcatca ttacgcctgc ttcgcgggac
     4381 tcgcggtaga cctcttctct attttctaaa tagaattcac tgtcgtgaat gtggcaatgt
     4441 gtgtcgatga tcatatttat tctgcctttg gtgctcgtgg atcttgggtg tgaagataga
     4501 tcttcgggaa gagtggctcg agagtatttg gtacgacacc actattgaat atctgatgga
     4561 tcttttctgc ggttgacggc ataaacggtg taatgaagtc ggcaatctga gacaatgtgc
     4621 ttgcagaatg ggcaagcact tcagctagat gagattcagc atctggatct ttaccgagct
     4681 gctttgcaat tatccaaggt ttcacatttt caaggtactg attcagcgcg cgaaccgtgt
     4741 tccaggcgat gtcgagggct ttattgaatt caaattcagc catcgcctcg cggtatggcc
     4801 ccatatcgtg ctcggctttt ggcatgtcgc caataacacc tgcctgatag cgctgaatca
     4861 tcgaggcaac acgttgtacg agattgccaa gatcgttacc gagctcgtta ttatatgagt
     4921 tctcgaattt ctcccaggta aagtcaccgt cttcctgcgt cggtacgtgg cggctgaagt
     4981 aatagcggaa tgcatcagtg ccataggagt tgattacttc catcggaccg acaccattgc
     5041 cgagactctt gctcatcttg gcgccaccga ctgttacaaa cccatgtacg aggagtacct
     5101 ttggcaatga aatgcctaag ccaagtaaca ttgcgggcca gataccagca tggaagcgca
     5161 ggatatcttt accaactact tgtacgtcag ccggccagta ttcttgccat tcttctggtc
     5221 ggtccgggt
//
LOCUS       DATIIX010000047         3124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_261196, whole genome shotgun sequence.
ACCESSION   DATIIX010000047 DATIIX010000000
VERSION     DATIIX010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261196"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VLF64_01405"
     CDS             <1..343
                     /locus_tag="VLF64_01405"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HSW90633.1"
                     /translation="AIAFAAQAVNWADVFFTAPGVILLVLSGLQMALYCDECTQGFAT
                     RWLDVALGLFSISGVLWTFLLIYQNRLLRELEEGSKEFYKTLHRWYIVGAANTIIPII
                     ILAIMTVKPSF"
     gene            471..878
                     /locus_tag="VLF64_01410"
     CDS             471..878
                     /locus_tag="VLF64_01410"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HSW90634.1"
                     /translation="MKKVAKLVIIDKDKKYLLLYRNDHPVFGNDPDLPGGTLEGLEST
                     LETMIREVQEEVGILVDKDAVEEIYSGVEYSAHGTSYSLFITQLIDRPTVNISWEHAS
                     YDWQGRASFLAKARGASDTYMHMVGETLTKMGL"
     gene            1041..1211
                     /locus_tag="VLF64_01415"
     CDS             1041..1211
                     /locus_tag="VLF64_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90635.1"
                     /translation="MTRLLHGKTFHDLFVHNGPERFGGKGVEWLDSQIQELYSEADQR
                     RNGVVGIEFEIV"
     gene            1292..>3124
                     /locus_tag="VLF64_01420"
     CDS             1292..>3124
                     /locus_tag="VLF64_01420"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HSW90636.1"
                     /translation="MGKFLRSAAGGAYRHKWLVIAGWIIILITLGMLAKHFYKQPSSA
                     ISIPGTSAQKALDRFGELFPSAGKGSGRVVFEAKNGETITQHKTGIEAAITKISKVEG
                     VRQVTDPFAVTGSISSDNTVAYATVGLTEEAAAVSEKTITDISKVGDDAQSKNFAMYR
                     GGDIISRVPSDILGPGEITGVVLALIVLLIMLGSLVAAGLPIIVAIVTIVSGAAGLFA
                     LSKTVNITSTTPVLAVMLGLAVGIDYSLFIVNKYRHYLLAGYDYEAATSRALATAGKA
                     VLFAATTVVIALSALSVVGIPFMTSMGLAAAGTVAIAALVAVTLLPALFGVVGSRIFS
                     RSIRRKIIAAQKRGPHESHRAARGVLWRKIAAILTNHPLMVVFIGVVVAGVIALPIRS
                     LNLGLPTDEFAAGNTSQRHAYDALKHGFGVGFNAPLIVIAENIPTVTGTDRQAARDQL
                     TAVYNQRISEATTTQTLTFQRQMAEAKTYAERLQVQKDIATAQAAGLQQKHQAQQQLE
                     AQIEQYAVRYELAKIANNIARRSDVQSATPALVTADTTKGMIQVIPKSGPSDQATKKL
                     IGDLRNNNTAASLAGTTTILGVTGSAALQMDINDKLAGVLPTYLG"
BASE COUNT          873 a          689 c          768 g          794 t
ORIGIN      
        1 ggcaatcgcc tttgcagcac aagctgttaa ctgggcagac gtcttcttca ccgctcctgg
       61 tgttattcta ctcgtgctta gtggtctgca gatggcccta tactgtgatg aatgtacaca
      121 ggggttcgca acgcgttggc ttgatgtcgc tcttgggcta ttttctatct ctggcgttct
      181 atggaccttc ttattgatct atcaaaaccg attacttcgc gaacttgagg aaggctcaaa
      241 ggaattctac aaaaccttgc atagatggta tatcgtgggt gctgcgaata caattatccc
      301 catcattatc ctagctataa tgactgttaa gccaagcttc tagtagttgt tgtttccagc
      361 cttgaggcat acccaacggc gctctcgact catccgcatt atccccgttc tcgtcaatca
      421 ggaaccaaac tcttgttctg gttggctcgt gctactatga atacatacac atgaaaaaag
      481 tcgcaaagtt agtcattatt gataaggata aaaagtatct attactatat cggaatgatc
      541 atccggtatt tggtaatgac ccagatttgc ctggtggtac gctcgagggc cttgaatcta
      601 cactcgagac gatgatccgt gaagtgcaag aagaagtggg tattcttgtc gataaagatg
      661 cagtcgaaga gatatattct ggtgtggaat actctgcaca cggtacatcc tactcattgt
      721 ttattactca attaattgat cgacctacag taaatataag ttgggaacac gcatcatatg
      781 attggcaagg gcgagccagt ttcttagcaa aagcacgagg agctagtgac acttacatgc
      841 atatggtcgg cgagacattg acgaaaatgg ggctgtaatg cagcatcaac tacaacttgc
      901 agcagaacca tttgcagcaa ttacgagtga taagaagaca attgaatcga gatcgctcga
      961 tgcaaagcgt caagattata gttggcaatg aaatcatatt tacgaatcgt gaagatacca
     1021 gtcaaacaat acgggtaaaa gtgactaggt tacttcacgg caaaacgttt catgatttat
     1081 ttgtccataa tggccccgaa aggttcggtg gcaaaggtgt ggaatggctc gatagccaga
     1141 tccaagaatt atatagcgaa gctgaccaac gtagaaatgg tgttgttggt attgagttcg
     1201 agattgttta atatatccta acatgttcag actaacattg ttgtgcaggg cataagtgct
     1261 acaatgagag gtaataacga gcggaggggc aatgggtaag ttcttacgat cggccgcggg
     1321 tggcgcgtat cgacataaat ggctggtgat tgctggctgg ataatcatac taattacatt
     1381 aggcatgctt gcaaagcatt tttataaaca gccaagtagt gcaattagta tccccggaac
     1441 ctctgcgcaa aaagcgcttg atcgatttgg cgagctgttc ccaagtgccg gtaagggaag
     1501 tggtcgggtg gtatttgagg cgaaaaatgg tgagacaatt acacagcata aaacagggat
     1561 agaggctgca attacaaaga tcagtaaggt tgagggagtt cgccaagtta ccgatccatt
     1621 tgctgttact ggctcaatat caagtgataa cacggtcgca tatgctacgg tgggtctaac
     1681 tgaagaagcc gctgcagtca gtgaaaagac catcacggat atctcgaaag tcggtgacga
     1741 cgcacagtca aagaactttg ctatgtaccg tggtggcgac atcattagtc gtgtgcccag
     1801 tgatatttta gggccaggtg aaatcacagg tgtagtattg gcacttatcg tactgcttat
     1861 tatgctcggg tcattagtag cggctggact accgatcatt gttgcaatcg taacgattgt
     1921 aagcggcgca gccgggctgt tcgcactgag caaaacagtg aatattacct caacgacacc
     1981 agtactagcg gtgatgcttg gcttagctgt cggtatcgac tactccctct ttatcgtgaa
     2041 taaataccga cattatttgc ttgccggcta tgattacgaa gcggccacaa gcagggcgct
     2101 cgctactgcg ggcaaagcgg tattatttgc agcgactacc gtagtaattg cactatcagc
     2161 gctgagtgtt gtgggcattc catttatgac aagtatgggt ctcgcggctg ctggtacagt
     2221 tgcaattgca gcgcttgtcg cagtaacgtt gttgcctgca ctattcggcg ttgtagggtc
     2281 acgcatcttt tcacgctcaa ttaggcgcaa aattattgcc gcacaaaaac ggggcccaca
     2341 cgagtcacac cgagctgctc gtggtgtact gtggcgtaaa atagcagcca tactaaccaa
     2401 tcatccgctc atggtagtat ttatcggcgt agttgtagcg ggtgtaattg cccttccaat
     2461 tcgttcgcta aatctcggcc tccctactga tgagttcgca gccggtaaca catcacagcg
     2521 tcatgcatat gatgcactta agcatggctt tggtgttgga tttaacgcgc cactcattgt
     2581 cattgccgaa aatataccaa ccgtgacggg tactgatcgc caagcggctc gcgaccaact
     2641 tacggcagta tacaatcaac gtattagcga ggccaccact acgcaaacgc tcacattcca
     2701 gaggcaaatg gccgaggcta agacctacgc agagaggttg caggttcaaa aagatattgc
     2761 aactgcacag gctgctggtt tgcagcaaaa acaccaagcc cagcagcaac tagaagcgca
     2821 aatagagcag tatgcagtgc gctatgagct tgcaaaaatc gcaaacaata tcgctcggcg
     2881 aagtgatgtg caatcggcga cgccagcgct cgtgacggcc gacacgacca aggggatgat
     2941 ccaggtaatt ccaaaatctg gaccttcaga tcaagcaact aagaaattga tcggcgattt
     3001 acgtaataat aacacagcag caagcctagc aggtactact acaatacttg gtgtaacagg
     3061 ctcagcagcg ctacaaatgg acatcaacga caagctcgca ggcgtcctgc caacatactt
     3121 agga
//
LOCUS       DATIIX010000048         3195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_38909, whole genome shotgun sequence.
ACCESSION   DATIIX010000048 DATIIX010000000
VERSION     DATIIX010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38909"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VLF64_01425"
     CDS             <1..553
                     /locus_tag="VLF64_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90637.1"
                     /translation="SWPDPSKLVFHESGSAALAGIGAEKAILSGVRAIKRGYVPKTGE
                     FIEHPVFSGGDRRVEDRASGTIWAAFGMSGSYTCIRWFDEYPVAFGISQEDLIEYRRQ
                     QGHKIPELFETGEGIMAGPEIPLALARVIYVPKQAILGAKTWVAENCTPGTRAISLEA
                     AQMYGTVTSKMSELLAQDPVIIR"
     gene            complement(626..1225)
                     /locus_tag="VLF64_01430"
     CDS             complement(626..1225)
                     /locus_tag="VLF64_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90638.1"
                     /translation="MFQSVIGVIAGVLAFVAFIPYAISIFRGKTKPQRSTFAIWSIIS
                     LVTLFSYFASGARQTIWIALVYAILQLFIFVLSFKYGMGGLSRLDIICLSGALIGVLA
                     WVVTDNPAIALYASITSEFLGYVPLFKKAYIHPETESTLSWILGLLAACLNLFALTTL
                     RLDIALYPMYVVFSDGLVVALLILSRRRLRREDAQAKNA"
     gene            complement(1268..2245)
                     /locus_tag="VLF64_01435"
     CDS             complement(1268..2245)
                     /locus_tag="VLF64_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90639.1"
                     /translation="MATPTLIRPATSVDADASTSKDRLYDVFNNPFGSIAGEPQFHGG
                     LDALDLESGLDGAERMHRFAVACGGVAVSATQEVLKRPAVLQTIFEHRLHAFWSKGDL
                     QLGGDPTKDEITDAVRAISTPEELEESVIRRAQRLGENGASRIMVIMRGAALYADYNG
                     MSFVQEGRLFDVLRRSYHLASPIAVLHDDRESGGRTGDPYTNIGLLHKICSGRRGVRE
                     LTNGNDMRNVMAPLDDPDNQPIIVQPGRFRMKTYARPGIDTETNQGMMVEIDLPRGEE
                     QIIFRRLPKHTVTISTPQRGCPVQTQGSMQALWVRAANAYELAAATSGL"
     gene            2386..>3195
                     /locus_tag="VLF64_01440"
     CDS             2386..>3195
                     /locus_tag="VLF64_01440"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HSW90640.1"
                     /translation="MMHSNEIKIEADLVRELLKVQFPKWADLPLSPITPSGTDNAMFK
                     LGDALAVRLPRTEHAAQNIEKECRWLPKLAPHLPLPIPAPVAQGKPHEKYPLPWAICH
                     LLVGENLNVSHLDDLHQAAIDMGEFVSALQQIDVASGPINKRGLPLNTRDEETRAAIT
                     QLEGVIDVDRAASAWESMLALPQWNGLPTWLHGDLHPGNLLVKAGRISAVIDFGSCGV
                     GDPAADLMFAWAVLDTKSREVFRSIVKPDEASWARGRGWAFTMGIVAYPYYQ"
BASE COUNT          812 a          802 c          812 g          769 t
ORIGIN      
        1 atcctggcca gacccaagca aattggtctt ccacgaaagc gggtccgccg cactcgccgg
       61 cattggagcg gagaaagcca ttctgtctgg cgtacgcgct attaaacgtg gctacgttcc
      121 taagactggc gaatttattg aacatccggt tttcagtggc ggtgatcgga gggtagaaga
      181 tcgcgcgtca ggcaccatat gggcagcatt tggcatgtct ggctcgtaca cctgcattcg
      241 ttggtttgac gaatacccgg ttgcgttcgg catttctcaa gaggatctca tagagtaccg
      301 taggcagcaa ggccataaaa tcccagagct tttcgaaacc ggtgaaggta tcatggctgg
      361 cccggaaata ccgctggcac tagcaagagt tatctatgtg ccgaaacaag caatcttggg
      421 cgcaaaaacc tgggtagccg aaaattgcac acctggtact cgagcgatat cactcgaggc
      481 ggcacagatg tatggcactg tcacatccaa gatgtccgag ctacttgccc aggacccggt
      541 aattattcgt taatatccgt tcgacctagc aagtcaaaca atccaaggtt ttaattcatc
      601 gacatgttgt gggttaacga aaagattaag cgttctttgc ctgggcgtcc tctctacgta
      661 gccgccgtct tgaaagaatg agtagcgcaa caacaagtcc atcgctaaac actacgtaca
      721 taggatatag ggcgatatct aaacgtagtg tcgttagtgc aaaaaggttc agacaggcgg
      781 ctagcagccc gagaatccac gatagagtac tttccgtttc cgggtggata tatgctttct
      841 taaaaagagg cacatagccc aaaaattctg atgtaatgct tgcgtatagc gctatagcgg
      901 gattgtctgt gactacccag gcgaggacac cgatgagcgc tccgctcaag caaataatat
      961 ccagcctgct aagtcctccc atgccatatt tgaacgacag aacaaatata aatagctgca
     1021 gtatggcata caccaatgca atccagatag tttgcctagc tcctgatgca aaataactaa
     1081 ataacgtgac aaggcttatg atggaccaaa ttgcaaaagt agaccgctga ggtttagttt
     1141 tacctctgaa aatgctgata gcataaggga taaaggcgac aaacgcgagc accccggcaa
     1201 taaccccaat gaccgattga aacatgtcct tatgataaga gatgctgcgt cgcaaagcca
     1261 gatatggcta taaaccagac gttgcagccg caagttcgta cgcattggcc gcgcgtaccc
     1321 acaatgcttg catacttccc tgggtttgca ctgggcaacc acgctgtggc gtactgattg
     1381 taacggtgtg cttaggcaag cgtcgaaaga taatttgttc ttcgccccgc ggcaaatcta
     1441 tttcaaccat catgccttga ttcgtttctg tatcgatccc tgggcgggcg tacgttttca
     1501 tccggaaacg ccccggctgg acaataatag gctggttatc aggatcgtct aacggcgcca
     1561 tgacattccg catatcgtta ccgttggtta gttctcgcac tccgcgcctc ccactgcaaa
     1621 ttttatgcag caacccaata tttgtatagg ggtcgcctgt tctgccgccg ctttcgcggt
     1681 cgtcgtgcag cacggcaata ggtgaggcaa gatgatagct acggcgcagc acatcaaaca
     1741 gtcgtccttc ttgcacgaac gacatcccgt tatagtctgc atagagcgcc gcgccgcgca
     1801 taatgaccat gatgcgcgaa gcgccatttt caccgaggcg ctgcgcccgt cgaatgacgg
     1861 actcctccag ttcttcgggc gtagagatgg ctcgcacggc atcggtgatt tcgtctttgg
     1921 tcgggtctcc gccgagctgg aggtcacctt tgctccaaaa tgcatgcaat cgatgttcaa
     1981 agatagtctg gagaactgca ggtcgtttta gcacctcctg cgtagcactt actgctacgc
     2041 caccgcatgc tactgcgaat ctatgcatac gctctgctcc atccaggcca gattcgaggt
     2101 cgagtgcatc gagtccccca tggaattgcg gctctcccgc tatgctgcca aaaggattat
     2161 tgaatacgtc gtagagccta tccttggagg tagaagcgtc cgcgtccaca gatgtcgccg
     2221 gacgtataag cgttggagtg gccataccgt acattctaga acaatctttg taattatatg
     2281 catcattttt aagaagtagt gacttacttt tggaacaggg cgtcggggca atagagattt
     2341 actagattcg ccgacaaatt actcagaaaa gctataatgg gcaatatgat gcacagcaat
     2401 gagataaaga tcgaagccga tcttgtgcga gaactactaa aagtacagtt tccgaagtgg
     2461 gccgatttac cactttctcc catcacgccc tccggaacgg acaacgctat gttcaagctt
     2521 ggcgacgccc tggccgtgcg tttaccgcgc actgaacatg ctgcccaaaa tatagaaaaa
     2581 gagtgccgct ggttgccgaa gttagcgcca catttaccct tgccgattcc cgcaccggta
     2641 gcccaaggta agccccacga gaagtatcct ttaccctggg ccatttgcca tttattggtc
     2701 ggggagaatc taaatgttag tcaccttgat gatctgcatc aggcagctat tgatatggga
     2761 gaatttgtat cggctttgca gcaaatagat gtggcgagcg gtccaatcaa taagcgagga
     2821 ctaccgctta atacgcggga cgaagagacg cgggcagcaa tcacccaatt agagggtgtt
     2881 atcgatgtcg atcgagcggc cagtgcctgg gaatcaatgc tcgccttgcc gcaatggaac
     2941 ggcttgccca cctggctcca cggcgatctt catcccggca atttgcttgt aaaggctggt
     3001 cggataagtg ctgtcattga ttttggctca tgtggagtag gtgatccagc cgctgatcta
     3061 atgtttgcat gggcagtgtt agatactaag agccgggaag tatttcgttc aatagtaaaa
     3121 cccgacgaag ctagctgggc gcgtgggcgt ggctgggcat tcaccatggg gatcgttgcg
     3181 tatccgtact atcaa
//
LOCUS       DATIIX010000049         4256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_150585, whole genome shotgun sequence.
ACCESSION   DATIIX010000049 DATIIX010000000
VERSION     DATIIX010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150585"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VLF64_01445"
     CDS             complement(<1..1161)
                     /locus_tag="VLF64_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSW90641.1"
                     /translation="MDRHRRYTVFLRQLQHKLRLRQPGFTLVEMLVVAPLVLIVIAGL
                     VSAMVAMIGDSLIANSRVNTAFSLQDTLNQIEQDTRVATNFMGVFSYLNSPQGRDGNT
                     GAFNYNSKEDLILTQQATTSSPYNTARDLVYYADQPAACGSNDKSGNRVLADRVLYFL
                     DTSTKTLWRRTVVNPWNLSSTHDGNTVCSTPWQRDSCPQGSTISNTPGSSCQSVDQKM
                     LDNVTDFTPTFYDSSNNVVTDPTQAVNVQVSITVQHNVSGSTLTQTSVMRATRRNDVP
                     APPVPDAPVVSIYNAGINSYNNPILTTFQWSSNGAYTYIVSTQINGGSWSADQSVSGT
                     TMGVSAPPGATINIKVTAVNDSGNSTTTTYSTVQDIFTDMNLGNSWVSFDSTT"
     gene            complement(1504..2106)
                     /locus_tag="VLF64_01450"
     CDS             complement(1504..2106)
                     /locus_tag="VLF64_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HSW90642.1"
                     /translation="MRRWTGFTIVELVVVMVIMAILLTLAGFTLNRSQGNSRDAERNA
                     DIAVIARGLETRYREGNPRSGLPAYVAPGSYPDVDEMAYIIGNTVSTFTPTQVIGGFS
                     NDALPGTSVPNFTPPQVSGGAYAGFAIATCTTAGSCASVNDSGASGSSTGSSTPVSQY
                     FYEPIDANGKICKTTTCVRFNLYWKTEVDNQTHILRSNRQ"
     gene            complement(2147..3172)
                     /gene="murB"
                     /locus_tag="VLF64_01455"
     CDS             complement(2147..3172)
                     /gene="murB"
                     /locus_tag="VLF64_01455"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HSW90643.1"
                     /translation="MDIHTDIPLKNYVTMRIGGNARFMTDIHSADDIPALVKRAASQN
                     LPIYILGGGSNTIVRDEGFNGLVLRNRIPGFEVIAETASDATIKIGAGENWDDVVRRS
                     VNMNLSGIECLTAIPGTAGAAPVQNIGAYGQEVAETLVELEAYDRKTDQMIILTNADC
                     GFAYRYSIFRGDEMGRYIITNITLKLYKTPPQPPFYKAVQDYLDAHTITLYTAQVLRD
                     AVTAIRADKLPDPKERPNNGSFFKNAVVEDWQLTTLREEYPDIPVYEMPDGRFKIPTG
                     WLIERAGFKGQLLHGMRVNDKNALVLINESATGYTDLAAARDEIIGAVRDKFRVQIEQ
                     EPLEMTI"
     gene            3263..3339
                     /locus_tag="VLF64_01460"
     tRNA            3263..3339
                     /locus_tag="VLF64_01460"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3297..3299,aa:Arg,seq:tcg)
     gene            3440..3979
                     /locus_tag="VLF64_01465"
     CDS             3440..3979
                     /locus_tag="VLF64_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90644.1"
                     /translation="MQYLLIVVVIMLITGLSYTVVKWPGGLHMTFSQHVIAPRWSKIY
                     YASLFLVTLPLFVWFIGSWLIPQKQLPGTFLWFTCVAALFQLACTWFPETGGWRTTVH
                     RVLTGVSGVAMLPLMFILATTSSISISVRFSAWAALGLMIILLVIAVSNQKGYKWALL
                     LQVGYYATFFAVLLLATYL"
BASE COUNT         1097 a          948 c         1048 g         1163 t
ORIGIN      
        1 ggtagttgaa tcaaaggaga cccaagaatt tcctagattc atatccgtaa agatatcctg
       61 cacagtactg taggtggttg tagttgaatt gcctgagtca ttcacggctg tcacctttat
      121 attaatggtt gcccccggag gcgccgacac gcccatagtc gttcctgaca cactctgatc
      181 cgcagaccat gagcccccgt taatttgcgt agacacgata tacgtgtaag caccattcga
      241 agaccactgg aaggtggtaa gaattggatt gttatagcta ttaataccag cgttatatat
      301 agagacaacg ggcgcatcag gtactggcgg agctggtaca tcattacgac gagtggcccg
      361 cataacactc gtttgcgtaa gagtgctgcc actgacattg tgttgaacag tgatggagac
      421 ttgtacgttt acagcctgcg tagggtctgt aacaacgttg ttcgaggaat cataaaaagt
      481 tggtgtaaag tcggtgacgt tatcgagcat tttctgatca accgattggc atgaggagcc
      541 tggggtatta ctgatagtac ttccttgtgg gcagctatct cgttgccatg gtgtgctaca
      601 tacggtatta ccatcgtgtg tactacttaa attccatgga ttaacgaccg tgcgtcgcca
      661 aagggtttta gtgctggtat caaggaaata aaggacacga tctgcgagca cacggttgcc
      721 actcttatca ttgctaccac aggcggcagg ctggtcggca taatagacaa ggtctctagc
      781 agtgttatac ggacttgatg tcgttgcttg ctgggtgagg ataagatctt ctttactgtt
      841 gtagttaaat gcacccgtat tgccgtcgcg gccttgaggt gaattgagat agctgaatac
      901 acccatgaag tttgtggcga cgcgcgtatc ttgctcaatt tggttaagcg tatcttgaag
      961 actgaaagcc gtattaacgc ggctgtttgc gatgagcgaa tcaccaatca ttgccaccat
     1021 tgcgctaaca agtccagcaa tcacaatgag cacaagtgga gcaacaacaa gcatctccac
     1081 aagagtgaag cccggttgcc gaaggcgaag tttatgctgg agctgtcgta agaacacggt
     1141 ataccttctg tgtctgtcca ttactagtat aagttatggt tacagtgagt tttgtaagat
     1201 ttggggaagc tggattatat gggcatgtaa tatctacatg cgcagttgca ccttggaggt
     1261 gcgcgtatga cggaactgct ggattgtctg tatccgccca acagggcgta tatgcccagt
     1321 aggggttttc ttgatattgg cgtaataaat cataggccgc attacttgcg agcgatcggg
     1381 cggtagcatc ttgtgattgg cggatcacgt taagatacag ttgataggcg gatataaata
     1441 gcagagaccc cacaacaatc gcaattagga gctcgactgc agtgaaccct tgaactcgcc
     1501 ggttcattga cgattgctcc gtaaaatgtg tgtctgattg tcgacttcag ttttccagta
     1561 caaattaaag cgtacgcaag tggtagtttt gcagatcttt ccatttgcat cgataggctc
     1621 gtagaagtat tggctaacag gtgtagagct accagttgaa gatccgcttg ccccggaatc
     1681 attaacactt gcgcatgagc ctgcagtcgt gcaggttgca atggcaaagc cagcataggc
     1741 tccaccagaa acttgcggtg gggtgaagtt aggcactgag gtaccaggaa gtgcatcatt
     1801 tgaaaacccg ccaatgacct gtgttggtgt gaaggtgctg acagtattgc caataatata
     1861 agccatctcg tcaacatcag ggtatgaacc aggtgcaacg taggcgggta gtccacttct
     1921 cggattacct tctcgatatc gtgtttcgag tccgcgggcg ataacggcga tatctgcgtt
     1981 gcgctcggcg tcacgagaat tgccctgtga tcggttaagg gtaaacccag ctaaggttaa
     2041 tagaattgcc atgatcacca tgacaacgac taactcaaca atagtaaacc cagtccagcg
     2101 ccgcatacat atagcataag tggtttgtga gtgaaagaca agggaattaa atcgtcattt
     2161 caagcggctc ttgctcgatc tgtacgcgaa atttgtcacg aactgcaccg ataatctcat
     2221 cacgggcggc agcaaggtca gtgtagcctg tcgccgactc gttaatgagt acaagggcat
     2281 ttttgtcatt gacgcgcatg ccatgaagta attgtccttt aaatcctgcc cgttcgatga
     2341 gccaacctgt cggaatttta aaacggccat cgggcatctc atacacgggt atatcagggt
     2401 attcctctcg tagcgtggtg agttgccaat cttctacaac agcattctta aaaaatgaac
     2461 cgttatttgg acgctcttta ggatcgggga gtttatcggc acgaatggct gttacggcgt
     2521 cgcgcaagac ttgcgccgta tagagtgtaa tagtgtgcgc atcaagataa tcttgcacag
     2581 ccttataaaa tggtggctgc ggtggggttt tataaagttt cagagtaata ttcgtaatga
     2641 tatatcgtcc catttcatca cccctgaaaa tactatatcg ataggcgaag ccacagtcgg
     2701 catttgtaag gataatcatc tggtcggttt tacggtcgta ggcttcaagc tcaacgagtg
     2761 tttcggcaac ttcctggcca taggcaccga tattctgtac tggtgcagcg cctgcagtac
     2821 caggaatagc agtgaggcat tcgatgccac tgaggttcat attaacactg cgacgcacaa
     2881 catcatccca gttctcaccg gcgccaatct taattgtggc atcagatgct gtttcggcaa
     2941 tcacttcaaa tcctggaata cgattacgaa gcacgaggcc attaaagcct tcatctcgaa
     3001 caatagtatt actaccacca ccaagtatat agatagggag attctgcgag gcagcccgtt
     3061 taacaagggc aggaatgtcg tcggcagagt ggatatcagt cataaatcgt gcattaccac
     3121 caatgcgcat agtaacgtaa tttttgagag gaatatcagt gtggatgtcc atatactgta
     3181 agtatacact agcaatagtg tactttgtgc ctgaattagt atagcgatca atctgttaga
     3241 tatggtatac taatcgcagt ttgcacccgt agttcagcgg attagaatgc ttggcttcga
     3301 accaagtggt cgcaggttcg aatcctgccg ggtgtaccaa gaaaaatagc ctatctacca
     3361 tctaaagata ggctattttt cttggtaggt ttgcttagtg aatccgagcc atcaaaccct
     3421 ggtatactat actttatcta tgcagtatct attgatagta gtagttatta tgcttattac
     3481 cggccttagc tacacggtcg ttaagtggcc aggcggtttg cacatgacgt ttagccaaca
     3541 cgtgatcgcg cctcgctggt cgaaaatcta ctatgcgtcc ttgtttctcg tcacactacc
     3601 gctcttcgtg tggtttattg gtagctggct catcccgcaa aagcaactcc caggcacttt
     3661 tttatggttc acctgcgtcg cggctctatt tcaacttgca tgtacctggt ttcccgaaac
     3721 aggtggctgg aggacaaccg tgcaccgggt gctcactgga gtatcaggcg ttgctatgct
     3781 gccgcttatg ttcatactag ctacaacctc aagcatttca atctctgtaa gattctctgc
     3841 gtgggccgca ttaggtctca tgatcatatt actagtaatc gcagtaagca accaaaaggg
     3901 ctacaagtgg gcattgctat tgcaagttgg atactacgca actttctttg ccgtactact
     3961 tctcgcaacg tacctgtagc tacccctcaa tgagagcatc agagtgagtg gtttttagta
     4021 taagttgcca tgtgtgccga gcatgttaac gacctgacgc ttggcagata gctgaggcct
     4081 ttattgattt tgtctgcaat agcaaagcgt tgcccaatac gaaaatgctt gcattgttct
     4141 gtaagcaagc cgaggagtga ggcgatacgc tgcccaactc tttacatttt cgcccaaatt
     4201 agctataatg tctcaagtta cgggccagta gctcagctgg ttagagcacc tgcctc
//
LOCUS       DATIIX010000050         3846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_178536, whole genome shotgun sequence.
ACCESSION   DATIIX010000050 DATIIX010000000
VERSION     DATIIX010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178536"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VLF64_01470"
     CDS             <1..609
                     /locus_tag="VLF64_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HSW90645.1"
                     /translation="KTIVTRNIGIYSGTFDPIHIGHNAFANEAMRELELNAIILLPEA
                     QPRGKLHVTDIYHRVELIKRAIRGNTKLQVLTLPSKQFTVAQTLAELRSHFKDAHFTF
                     LIGSDVVYTFTYRWDSLEVLLKEATFAVGLRSGDSQDRLETIFKKLESQYKLPILRRY
                     IITKNTHITSSQFRQGKIDTTHLPHPAMASYIQENRLYTNLI"
     gene            complement(621..1346)
                     /locus_tag="VLF64_01475"
     CDS             complement(621..1346)
                     /locus_tag="VLF64_01475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HSW90646.1"
                     /translation="MRRNIQQHGQHFLRGRAFIAELIGHSNLRKNDTVLDIGAGSGAI
                     ASVLASRAKHVIAYEPEPVANALLRQNMQHNDNITVLQEDFLKAELPDAAYKVFSNIP
                     FHLSSEIVRKLTSAPRQPKAIYLIVQKQFAQKLIVSGDHFHGALGMAIAPWWAVRIRR
                     LLRKTDFTPPPAVDTVLLELKPHEMPLLDVSQQAAYTDFVMECYAAPRFYVAAGGNKN
                     KRPSTLAPADWVRLFTQYSKKRT"
     gene            1429..2829
                     /locus_tag="VLF64_01480"
     CDS             1429..2829
                     /locus_tag="VLF64_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HSW90647.1"
                     /translation="MSNLYTWLNDLVGSHKKKDSTIVREQNIAHARKKYNRVNRFIRV
                     LGPGVVTGAADDDPSGIATYSQAGAIYGLRLLWMFPIMFPLLLAVQESCARIGAVTGD
                     GLAAILKRHYGKKLLFSAVMLAAVANTVNIGADLGAMAATTQLLFPHVEYVSLSILFA
                     LICVLLQVFVPYRQYAKFLKWLAVVLFAYPITAFLVHEDWGGIVAHTFTPDFHINREM
                     MYIMVGMLGTTISPYLFFWNTSEVVEDEVNEHRIGISSGIGKPVISRRFIKKIRLDTA
                     AGMSLATISAWFIVIACAAVLNSHGITKIENPQDAASALEPLVRGFPNSGLIAKVLFS
                     VGIIGTGLLAVPVLAGSSSYAISEAFGIREGLYRKFGKAHGFYGIIAIATLAGLIINF
                     LGIDPIQALIFTAVFNAVAAVPLLFMIYRVGNNPEIMGEYKNSKWSNAGVLTAFILMT
                     IATIVLFASFIPRLHS"
     gene            2858..>3846
                     /locus_tag="VLF64_01485"
     CDS             2858..>3846
                     /locus_tag="VLF64_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90648.1"
                     /translation="MIKTIRYETLTLDKIVGGGQTLGTLTDGRKAFVWGGLPGETVTI
                     RVTKKKSHFIEGVVTEVIAPSSERIAPQDPESFISTSPWQIMKLENEQMYKAALIEEA
                     FELHNIVLPSPIEVYTNEIIYEYRNKMEYSWFSSTDATSGTDTLDLAFFARGSKGKYP
                     VDHSSLARPEITALAKAVRDLLRAKQISARQLKTLLIRSDQTGNAIWQLYLKDEIKDI
                     ITKTEAATLPAIGGEVIYSNPKSPASIITKRLASFGTTTLEDTILGTPFRYAVEGFFQ
                     VNIPVYEQSLRDMSEWINNKPTIDLYSGVGSIGLTIGSNNTTLIEINEHAVRE"
BASE COUNT         1095 a          975 c          806 g          970 t
ORIGIN      
        1 aaaacaatag tgacacgcaa catcggcatc tattctggca cattcgaccc aattcatatt
       61 gggcataatg catttgcaaa tgaagcgatg cgtgaactag aactcaatgc aataatactc
      121 ttacctgagg cgcaaccacg cggtaaactg cacgttactg atatttatca tagggtcgag
      181 cttataaaac gggccatccg gggaaatacc aagttgcaag tcctaacttt accgtcaaaa
      241 caatttaccg tcgctcaaac acttgcagag ctacgaagcc atttcaagga cgcgcatttt
      301 acgttcctta taggatcaga tgtcgtctat acattcacat acagatggga tagtctagag
      361 gtgctcttaa aagaagctac ttttgcagtt gggctccgaa gcggtgatag ccaagataga
      421 cttgaaacaa ttttcaaaaa acttgaatca caatataaac tacctattct gcgtcgctat
      481 attatcacca aaaacacgca tatcacgtca tcacagtttc gccagggcaa gatcgatacc
      541 acgcatttgc cacatcctgc aatggccagt tacatccagg aaaatcggct ctacaccaac
      601 ttgatataaa tcatcggaaa ctatgttcgt tttttagaat attgcgtaaa taggcgcacc
      661 caatcagcag gcgcaagtgt cgaaggccgt ttattcttat taccgcccgc ggcaacgtag
      721 aatcgcggtg ccgcatagca ttccataaca aaatctgtat acgcagcttg ctgagataca
      781 tcaagtaacg gcatctcgtg cggcttaagt tccagcagta cggtatcaac agcaggtggc
      841 ggagtaaaat cagttttacg aagcagccta cgaatacgca ccgcccacca cggcgcaatt
      901 gccatgccaa gtgcaccatg aaaatgatcg ccactaacaa ttaacttttg cgcaaactgc
      961 ttctgtacaa tcaaatagat cgcctttggt tggcgtggtg cactggtaag tttacgcaca
     1021 atctccgaac taaggtgaaa ggggatattc gaaaagactt tatatgccgc atcgggtaac
     1081 tcagctttta aaaaatcctc ttgtagcacc gttatattgt cattatgttg catgttttgg
     1141 cgcagcaatg cattagccac aggttcaggt tcgtaggcaa tcacatgttt cgcacggctt
     1201 gcaagcacgc tagcaatcgc gccactcccc gcgccaatat caagtacggt atcgttcttg
     1261 cgcaggttac tatgcccaat gagttcagca ataaacgctc ggccgcgtaa aaagtgttgt
     1321 ccatgctgct gtatatttcg cctcatatct ttacagtata ctaaataatt gccttatata
     1381 ctgcttatgc catacaatag acgcatatta tcgtaaacag agaaacgtat gtccaactta
     1441 tacacgtggc tcaatgatct cgttggctcg cacaagaaaa aagattctac tattgtgcgc
     1501 gagcagaaca ttgcacacgc acgcaaaaaa tacaaccgcg ttaaccgctt tatccgcgtc
     1561 ctaggcccgg gcgtcgtcac aggtgccgct gacgatgatc caagcggcat cgccacttat
     1621 agtcaagctg gtgcaattta cggactgcga ctcctgtgga tgtttccaat aatgttcccg
     1681 ctgcttctcg ccgtacagga atcatgcgcg cgtatcggag ccgtgacggg tgatggcctc
     1741 gcagcaattc tcaagcgaca ttatggcaaa aaactgcttt ttagtgccgt catgctcgca
     1801 gctgtggcta ataccgtaaa catcggtgcc gaccttgggg caatggcagc aactacacaa
     1861 ctattattcc ctcacgttga gtacgtatca ttatcgatcc tatttgcact catatgcgtg
     1921 ctgcttcaag tgttcgttcc gtaccgtcaa tatgcaaaat tccttaaatg gcttgcagtg
     1981 gtactgtttg cgtatccaat caccgcattt ctagtccatg aagactgggg aggaatcgtt
     2041 gcgcatacct tcacaccaga cttccatatc aaccgtgaaa tgatgtacat aatggtaggc
     2101 atgcttggta caactatttc gccatatttg tttttctgga acacaagcga agtagttgaa
     2161 gatgaagtca acgaacaccg tattgggatt tcttctggca taggtaaacc tgttatttcg
     2221 cgtcgattca tcaagaaaat tcgtctcgac accgccgccg gcatgtcgct cgcaacaatc
     2281 tcagcgtggt ttattgtaat cgcctgtgcg gcagtactaa actctcatgg catcacaaaa
     2341 atcgaaaatc cacaagatgc cgcctctgcg ctcgaaccac tcgtacgagg ctttccaaac
     2401 tctggactaa tcgcaaaagt tctcttcagc gttggaatta tcggtaccgg gcttttggcc
     2461 gtaccagtac tcgctggttc atctagctat gcaattagcg aagcgttcgg tatcagggaa
     2521 ggactctacc gcaagtttgg caaagcccat ggcttttacg gcatcatcgc cattgcaaca
     2581 cttgccgggc tcattattaa ctttctcggc atcgacccaa ttcaagcact aatcttcacc
     2641 gctgtattta atgccgttgc cgcagtgcca ctgctgttta tgatctatcg cgttggcaac
     2701 aacccagaaa tcatgggtga gtataaaaac tcaaagtgga gcaatgcagg cgtcttaacc
     2761 gcatttattc taatgactat cgctacaatc gtgctctttg catcattcat tccgaggcta
     2821 cattcataat tccacctcta gtgatatact gataggaatg ataaagacta tccgatatga
     2881 aacattgact cttgataaaa tcgttggcgg cggccaaaca ctcggcaccc ttactgatgg
     2941 ccgcaaagca ttcgtgtggg gcggcctccc cggcgaaacc gttacgattc gtgtcactaa
     3001 aaagaaatca cactttattg aaggtgtcgt tactgaagta atcgcacctt caagcgagcg
     3061 catcgcacca caagatcctg aaagcttcat tagtacgagt ccgtggcaaa tcatgaagct
     3121 cgaaaacgag caaatgtaca aggccgctct catcgaagaa gcattcgagc tgcataacat
     3181 cgtgctaccc agtccaatcg aggtatacac caacgagatt atttacgaat accgtaacaa
     3241 aatggaatat agctggttca gtagcacaga cgctacatct ggtacggaca cgctagatct
     3301 agcgttcttc gcgcggggta gcaaaggcaa atatccagtt gatcactctt cgctcgcacg
     3361 gcctgaaatc accgctcttg caaaggcggt gcgtgattta ctgcgcgcca aacagatctc
     3421 tgcacgccag ctcaaaacac tccttattcg ttctgatcaa accggcaacg caatctggca
     3481 actataccta aaggacgaaa tcaaagatat catcaccaaa acagaggctg ctacactacc
     3541 agcaattggc ggggaagtta tctactctaa tcctaaaagt ccagccagca ttatcacaaa
     3601 gcgccttgca agctttggca caactaccct cgaagacaca atcctgggca caccatttcg
     3661 ttacgccgta gaaggcttct tccaagtaaa cattcctgtt tacgagcagt cacttcgtga
     3721 tatgagcgag tggatcaaca acaagccaac tatagaccta tacagcggtg tcggcagcat
     3781 cggtctaaca attggtagca acaacaccac tcttatagaa atcaacgaac atgctgtccg
     3841 cgaaat
//
LOCUS       DATIIX010000051        11165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_179520, whole genome shotgun sequence.
ACCESSION   DATIIX010000051 DATIIX010000000
VERSION     DATIIX010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 11165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11165
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179520"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="VLF64_01490"
     CDS             complement(<1..1362)
                     /locus_tag="VLF64_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSW90649.1"
                     /translation="MNLVTSLDQIKGVGPKTADQLRAAGLATVSDLLTFLPRKHEDFT
                     NVTNISDLKPGTVTIRARCEKISTRPVRRGLRLTTAVLADDTGKLNAIWFNQPYRVQQ
                     LSGTDDEFYFSGVFEYNYGKYQLTAPSAEIAKEMPVQAERLLPVYHAVHGLKSQMVRK
                     VLEQLRPLMGVLPETLPVNLISREHLMSRAAAISTMHFPKELSEVVAARERLAFEELF
                     QLLLASHLNKVENQKLQGFALGFEPETIKQFVQQLPFSLTDAQRRAAWDILQDFESSA
                     PMNRLLQGDVGSGKTVVAGIAARQAASHGYQTALLAPTEILAIQHAKTLDNLLSPFGV
                     TVGLLTGHVKGAARKGLYARIESGEVDVVVGTHAIIQDKVVFHNLGFVVIDEQHRFGV
                     DQRQKLLNKSGVLPHLLAMTATPIPRSLALTVYGELDVSVLNQRPKGRLPIKTQIISP
                     VSRP"
     gene            complement(1384..2160)
                     /locus_tag="VLF64_01495"
     CDS             complement(1384..2160)
                     /locus_tag="VLF64_01495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HSW90650.1"
                     /translation="MKKLIAFDLDNTLAESKSQIPDEMSYILGRLLEKYEVCIISGGK
                     FEQFVKQVINRLSVSDDQLGRLHIMPTCGTRYYRYDVIQKEWKLIYHNDLTDDQKKRI
                     RHAFEEGAKQLGIWEEEPYGEIIEDRGSQMTYSALGQEIGEMLGEEGIKRKYKWDPDM
                     KKRNALRDLIAPGLDDVEVRVGGITSIDVTLPGVDKAYGMNKLIDKLDMSKEEILFIG
                     DMLQEGGNDYPVKLMGIDTVEVRNYQDTPFVIQGILSVTE"
     gene            complement(2216..3436)
                     /gene="tyrS"
                     /locus_tag="VLF64_01500"
     CDS             complement(2216..3436)
                     /gene="tyrS"
                     /locus_tag="VLF64_01500"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HSW90651.1"
                     /translation="MTTLSEELMWRGMVNQMTLADITDLDKETRTFYWGVDPSADSMT
                     IGHMAPAMMIRHFIEHGYKAVLLVGGATGMIGDPDGKKQERDLKTLEEVAKNKAALIE
                     QYKTIFAGADFEIVDNYDWFKDFGYLDFLRQIGKHVSMTQMLDREFIKARIGEGGEGI
                     SYAEFSYALIQGFDFLHLFREKNATLQVAGADQWGNSIAGAQLIRRLESAEAHVFTTP
                     LVINKQTGVKFGKTEAGAVWLDPAKTSPYKFYQFWLNVDDETAEDLIKIYTLLDKSTI
                     DELIANHRENRSARALQRELAYRVTEIVHGESVVDQVRRATKVLFNEDGVRLSSLTSE
                     EIDMLAAELPVAERGNIIDVLVIAGATQSKGEARRLLMGGGVTVDGQKVNESYEITDT
                     SLIKKGKNSFVLVR"
     gene            3550..6219
                     /locus_tag="VLF64_01505"
     CDS             3550..6219
                     /locus_tag="VLF64_01505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HSW90652.1"
                     /translation="MVKSKRQARGVRVYSNLVSKRRTKKDAKARRKAEYLATLPKHPL
                     KRFFYRLHPKRMAKYWFSKRGAFTLLKGAGVLTVILVLFGLSLFAYYRKDLDQISPST
                     LAQRVKSTVTKYYDRNGKLLWEDKGSGNYTLVVDPKNISPCMGQATVAIEDKDFYKHG
                     GVSITGTLRAALSNAGGNATQGGSTLTQQLVKQVFFSDQAAQRGLAGIPRKIKEMILS
                     VEVERMYTKSQILGMYLNESPYGGRRNGVESAAQTYFGTSSSKLTLSQCALLAAIPNN
                     PSVYNPYNVAGHDALVEREHKVLDNMAELGYITKEEATTAKQVPILDTLKPLTDQLEG
                     IKAPHFVLMVRNQLEQELGKTVVGEGGLTVKTTLDVDLQTTLEQNMADIFSGKMTDRD
                     CSYTNCANYAGFSNGAAAIEDTKSGQLLALVGSRGFDYSGFGQDNAATGFIQPGSSIK
                     PLVYAQLFQNQGQGKANYGSGSVLSDDATTFAGGYKPQDADGKFQGGINLPQALDRSR
                     NIPAIKAMAVNEANNPGSTWATIRAMGDTQYCTQGADAQAGLSSAIGGCGTRLVDHVN
                     AIASLGRQGVYTPQSMILKVTNSTGQTLKEYKASSKQVIDPQSAYVVTDMLGDSKARA
                     GLGWNQDYLPYLDKLGVHTAAKTGTSNAQVGSKILPKDIWTVGYTPNLTMAVWLGNPD
                     TTPLKQGNSLIPAMFFDRTMATATTTYVNQGKAKYSDWYTAPTGIQNIKGELYPSYYN
                     KSAGVTNATMSFDKVSKKKATDCTPAGAKTDIGVSKTTDPITKQVVITAPDGYDPNSD
                     DDVHSCSDAQPTVSIAQSGSSLVVTVHSGTFTVQGVTLTENGNTIATQSGAGPWTFTA
                     SQLSSVTNGTVTATVTDEGYYTATNTVNYSSSS"
     gene            complement(6305..7285)
                     /locus_tag="VLF64_01510"
     CDS             complement(6305..7285)
                     /locus_tag="VLF64_01510"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HSW90653.1"
                     /translation="METRDIVLLAVTLIILGEVSYLLAKLPKSSTIKAKDRAILVDTS
                     VLMDGRITNIASTGFIGGTLVVPRSVIGELQLLADQADADKRARARHGLDVVSTLQGM
                     DGIEVEILQDGSKAAEGVDERLLTLAKRHSAVICTLDYNLNKVAVVEGIKVLNINELA
                     QSLRMQRLPGEQMMIELTQKGQDSHQGVGYLSDGTMVVVEQSNKYIGQTVQVEVVRSL
                     QTAAGKMMFARRVERQAPKKVEAPAIVVAKTKSLGRSRRNDSNETTKPKRAEPQLQQQ
                     SKQQKPQLSQNQPAQKSANQQKPRAVYHATRKRVNHEDSLLQLVDSQKDE"
     gene            complement(7301..8656)
                     /gene="radA"
                     /locus_tag="VLF64_01515"
     CDS             complement(7301..8656)
                     /gene="radA"
                     /locus_tag="VLF64_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HSW90654.1"
                     /translation="MAKTRSQFICQHCGASYPKWTGKCENCGEWNSLVEQIMQPVGSS
                     AVARGAHSGKVLSPQSMRSISAEETIQRMSTGFEDLDTVLGGGILPGGVLLLAGQPGI
                     GKSTLLLQVAAKIAEKQPVLYASGEESASQVKLRAARLGAVASDDLQFVSSTSADDIT
                     ATIRSGAYKVVIIDSIQTLSLDEITSAPGTVSQITNSSNVIIRAAKESGAAVILVGHV
                     TKEGSIAGPKVLEHLVDVVLQFEGDRYGGFKVVRAVKNRYGSTNEAAIFEMNESGLKI
                     VSNPSAALLAERQNADGSVVLATLEGNRPILVEIQALVNQSNFGYPKRTASGFDLNRL
                     NLLIAVLEKRTKLNLSDKDIYINVVGGLKLADPAADLAVAMAIASAAAGRKLDDGLVV
                     YGEVGLGGEIRSVAQADKRVAEAKKLGFTQAVAPVQPGKKNSFINGVRDIRAALIDYL
                     A"
     gene            complement(8659..9672)
                     /locus_tag="VLF64_01520"
     CDS             complement(8659..9672)
                     /locus_tag="VLF64_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90655.1"
                     /translation="MNNEPSQLPPQRPKHKILGGLLSLSVFAAIAAGAYFYGPIAVDA
                     IRASNFRPTSNIVGVNSRIALTARGQQIFYATTPAIEERDTFNTDCGSAERTAAILGC
                     YVRDQIYLYNIEGKELDGALEVTAAHEMLHAAYARLNIFEQENVNKMLNIEYAKIKDN
                     PDIKQEMQYYAQAEPGEELNELHSIIGTTIKDLPTDLEQYYTRYFTDRAAIVAMNAHY
                     TAVFDEVNQQATVLQQKITDEAAAIKQASSQYNADLTQLNSDIESFNEWATNGEFSSQ
                     YAFTTARQALAVRIEQMKNRQIALNQQIDTYNADVAEYNKLAVRAKQLNQSINGVEAP
                     NSI"
     gene            complement(9626..>11165)
                     /locus_tag="VLF64_01525"
     CDS             complement(9626..>11165)
                     /locus_tag="VLF64_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HSW90656.1"
                     /translation="ELHLIGATTLDEYRKHIEKDAALERRFQPVVVKEPTLKETIAIL
                     RGLKAYYEKHHSVAITDEVIESSVYMSDRYVSDRFMPDKAIDVIDEAAARVRVKQGHR
                     PSRQRELTKELKALNEKMEEAVSHEDYERAALYKQRVSQISEKIEVEKQAQEGKNTVA
                     LTDDDVAHAIAVMTNIPVEKVRSSEAKMLRHLEKHLSKQLIGQKEAITKVARAIRRSR
                     SGVASSKRPIGSFVFMGPTGVGKTELAKVLAKEVFGSQEALIKIDMSEFAEKHNTSRL
                     VGAPAGYVGYEDGGKLTDKIRRQPYSVVLFDEIEKAHPDVFQLLLQLLEEGKLTDAKG
                     REVSFRNTIIILTSNLGADKMMKESELGFHSLKQHNRELDTLHDRNASYAMEALEKFM
                     RPELINRFDGIVTFRALTREEVGKIFDLLIEELRQRLVRKGLGIAISAAAKRQLIDKG
                     YSAKFGARPLRRVIEDEVEHAIAERLLAGSDEKGAILEVGWSKGEVQIEQRTESVAAA
                     TSEA"
BASE COUNT         2706 a         2772 c         2565 g         3122 t
ORIGIN      
        1 tggtcgcgat acgggcgata ttatctgcgt ttttatcggc agccggccct ttggtcgctg
       61 atttaataca ctgacatcaa gttcaccgta cactgtaagc gcaagacttc gtggaattgg
      121 tgtcgccgtc atcgcaagta ggtgaggcag gacccctgat ttattcagca gtttttggcg
      181 ctgatctacc ccaaatcggt gctgctcgtc gataactacg aagccaagat tgtgaaagac
      241 caccttgtct tggatgattg cgtgcgtgcc aacaactacg tccacttcac cgctttcgat
      301 gcgtgcgtag agtcctttac gggctgcgcc ttttacatgt ccagtgagca atccgaccgt
      361 tacaccgaag ggcgatagta aattatcgag tgtttttgca tgttggatcg ccaaaatttc
      421 agttggagca agcagtgctg tttgatagcc atggcttgcc gcttggcgag ccgctatgcc
      481 tgcgaccact gttttgccgc tgcccacatc gccttgtaga agacgattca ttggtgcgct
      541 actctcaaaa tcctgtaaga tatcccaagc agccctacgc tgtgcgtcag ttaagctaaa
      601 tggtagttgc tgtacgaatt gcttgattgt ttcgggctca aagccaagag caaagccttg
      661 gagcttttga ttttccactt tatttagatg actcgcgagg agcaactgaa ataactcttc
      721 aaaagccaac cgttcacggg ccgccacgac ttcgctaagc tcttttggga agtgcattgt
      781 gctgattgct gcggcgcgtg acataaggtg ctcgcggctg ataagattga cagggagcgt
      841 ttcagggaga acacccatga gtggccgaag ctgctcgagt accttgcgta ccatttggga
      901 ttttagtccg tgaactgcat gatacaccgg cagcagccgt tccgcctgta cgggcatctc
      961 ttttgcgatt tcggcactcg gtgctgttag ctggtatttg ccgtaattat attcaaatac
     1021 accactaaaa tagaattcat catcagtgcc gctcaactgc tggacgcgat acggctgatt
     1081 gaaccagatg gcgttcaatt tacctgtatc gtctgctaat accgccgtcg taagccttaa
     1141 gccgcgtcgc actggacgag tactaatctt ttcgcaccgt gcgcgaattg ttaccgtacc
     1201 tggtttgagg tcggaaatat ttgtgacatt cgtgaaatcc tcgtgtttgc gaggtaaaaa
     1261 tgttaaaagg tcgctaactg tagcaagtcc ggccgcgcgc aactggtctg ccgtttttgg
     1321 ccctacgcct ttaatttggt cgagtgacgt tacaagattc atctattatc agtgtagcat
     1381 tggctactct gtaactgata agatgccctg tatgacaaaa ggcgtatcct ggtaattgcg
     1441 cacttcgaca gtatcaatgc ccataagctt taccggatag tcattgccgc cctcttgaag
     1501 catatcgccg ataaagagga tctcttcctt tgacatgtcc agtttgtcta taagcttatt
     1561 cataccatat gctttatcga ctccaggcag tgttacatca atacttgtga ttccgccgac
     1621 gcgaacttca acatcatcaa gtcctggtgc aataagatcg cgtaaggcgt tgcgcttctt
     1681 catgtcagga tcccacttgt attttcgttt aatgccctct tcgccaagca tctcgccgat
     1741 ttcttggccg agtgctgaat acgtcatttg cgatccacgg tcttcgatga tctctccgta
     1801 cggctcctct tcccagatgc caagttgttt tgcaccctct tcaaatgcat ggcgtatacg
     1861 ctttttttgg tcgtcggtaa gatcgttatg atatataagt ttccattcct tctgaatcac
     1921 atcatagcga taatatcgcg taccacatgt cggcataata tggagtctgc ccaactggtc
     1981 gtcagagaca ctcaagcgat tgatcacctg tttcacaaat tgttcaaact tgccacccga
     2041 aataatacaa acctcatatt tttcgagtag tctgccaaga atatacgaca tctcatccgg
     2101 aatttgagat tttgattctg ctaatgtatt gtcgagatca aacgcgataa gtttcttcat
     2161 tagtttctcc aaatttactt gtatcgaacc tttagtgtac cattatttgg accagctatc
     2221 ggacgagtac aaagctattc ttaccttttt tgattaaact tgtatctgta atttcataag
     2281 actcattgac tttttgacca tctactgtaa cgccaccgcc cattaataaa cgtcttgctt
     2341 cgcctttaga ttgtgtagca cctgcgatta cgagtacatc aataatatta ccgcgttcgg
     2401 ctactggaag ttctgcggcg agcatatcga tctcttcgct agtaaggctt gagagccgga
     2461 ctccatcttc attgaagagc acctttgttg cacgacgaac ctggtcgaca accgactctc
     2521 catggacgat ctctgttacg cggtacgcaa gctcacgctg caaggcacgg gcgctacggt
     2581 tttcacggtg gttcgcgata agttcgtcaa tcgtcgactt atcaagtaga gtgtagatct
     2641 ttatgaggtc ttcggccgtt tcgtcgtcaa catttagcca gaactggtag aacttgtagg
     2701 ggctggtctt ggcaggatcg agccatactg cgcctgcttc ggttttgccg aactttactc
     2761 cggtctgttt attgataact agcggagtgg taaatacgtg agcctcagca ctttcaagtc
     2821 gacgaattaa ctgagcacca gcgatgctat tgccccactg gtcggcgcca gctacctgca
     2881 gtgttgcatt cttttcgcgg aagagatgaa ggaaatcaaa tccctgaatt aacgcataac
     2941 taaactcggc atagctgatt ccctcgccgc cttctccgat acgcgcctta atgaattcac
     3001 gatcaagcat ctgagtcatc gaaacatgct tgccgatttg acgcagaaaa tcaagatatc
     3061 caaaatcttt gaaccagtcg taattatcaa cgatttcaaa atcagcaccc gcaaagatgg
     3121 ttttgtactg ctcgataagt gcagctttat ttttagccac ttcttcaagc gtctttaaat
     3181 cgcgctcttg cttcttgcca tctggatcgc cgatcattcc ggtagcacca ccgacgagta
     3241 atactgcttt gtagccatgt tcaataaagt ggcgaatcat catagccgga gccatgtgac
     3301 caattgtcat actatcggca ctcggatcaa caccccaata gaaagttcga gtctctttgt
     3361 ctaaatcggt aatatcggct agtgtcattt gattgaccat gccgcgccac atcaactctt
     3421 ctgatagtgt cgtcatgtga aactctcctt tctttaagta aactagtgtt atggtagcat
     3481 aaatgaccta gcgcgagcaa aaaaagagtg gtacaattaa tgtaatattg cttatgggaa
     3541 agtggtaacg tggtaaaaag taagaggcaa gcccgcggtg tacgcgtgta ttcaaatctt
     3601 gttagcaaac gtcgcacaaa gaaagatgcg aaagcacgtc gcaaagctga atatctagcg
     3661 acactgccaa agcaccctct gaagcgattt ttctacagac ttcatccgaa gcgaatggct
     3721 aaatattggt tttctaagcg aggtgccttt actctcctta agggggccgg cgttctgacg
     3781 gttatattag tgctctttgg actctcgctc tttgcttact atcgtaaaga tcttgaccag
     3841 attagtccga gtaccttagc tcagcgcgtg aagtcgactg taactaaata ttacgaccgt
     3901 aacggcaaac tcctctggga agataagggc tcgggcaact atacgcttgt tgttgatccg
     3961 aagaatatct caccatgtat gggtcaagca acggttgcta ttgaagacaa agatttttat
     4021 aagcacggtg gtgttagcat taccggcacc cttcgcgcgg cactaagcaa tgctggcggt
     4081 aatgcaactc agggaggatc aacacttacc cagcagctag tgaagcaggt gttctttagt
     4141 gaccaagcgg cccagcgggg cttggccggt attccgcgta aaattaagga aatgattctt
     4201 tctgttgagg ttgagcgtat gtacactaag agccagatct tgggtatgta tcttaacgaa
     4261 tcaccatacg gtgggcgtag gaacggcgtt gagtcagctg cgcagaccta cttcggtact
     4321 tcatcttcaa aacttaccct gtcgcagtgc gcacttcttg ctgctatccc aaataaccca
     4381 agtgtctata acccctacaa cgttgcaggc catgacgccc ttgtcgagcg cgagcataag
     4441 gtgctcgaca atatggccga gcttggttat ataacaaaag aagaggctac aacagctaaa
     4501 caggtaccga ttcttgatac ccttaaacca cttactgatc aacttgaggg aattaaagca
     4561 ccacactttg tgttgatggt acgcaatcag cttgagcaag agcttggtaa aacggttgtt
     4621 ggtgaagggg gtttaactgt taagactacc ctagatgtcg atctacagac tacacttgaa
     4681 cagaatatgg ctgatatatt cagcggtaag atgactgatc gcgactgtag ttacacaaac
     4741 tgcgcgaact atgcaggatt cagtaacggt gctgccgcca ttgaagatac aaaatctgga
     4801 cagctcctcg cgcttgttgg tagccgtggc ttcgactact cgggctttgg tcaggataac
     4861 gcggcaactg ggttcatcca gcctggctca tcaattaagc cacttgtata cgctcagctc
     4921 tttcaaaacc aaggtcaagg taaagcgaat tatggaagcg gatctgttct ttcggatgat
     4981 gcaacgacat ttgcgggtgg ctataaacca caagatgccg acggcaagtt ccaaggtggc
     5041 attaatcttc cgcaagcact cgatcgctca cgaaatatcc cagcaattaa ggcgatggcc
     5101 gtgaatgagg caaataatcc cggcagcaca tgggcaacta ttcgcgctat gggtgatacg
     5161 cagtactgta cgcaaggcgc cgatgcccag gccggcctgt cgagtgctat tggtggatgc
     5221 ggcacacgcc ttgttgatca cgttaacgcg attgcgagcc tcgggcgtca aggcgtctat
     5281 actccgcagt cgatgattct taaagttact aatagtaccg gccagacact taaagagtat
     5341 aaagcatcat cgaaacaagt tattgaccct caatctgcgt atgttgtaac ggatatgctg
     5401 ggtgactcta aagcccgtgc aggacttggg tggaatcaag actacctgcc atatctcgat
     5461 aagcttggcg ttcacactgc tgcaaaaact ggtacatcaa atgctcaggt tggtagcaag
     5521 attcttccta aagacatctg gactgttggg tatacgccga acctcactat ggcggtatgg
     5581 cttggtaatc cagacactac gcctcttaag caaggtaact cacttattcc tgccatgttc
     5641 ttcgatcgca caatggcaac agcaacgaca acatatgtaa accagggcaa agcgaagtat
     5701 agcgactggt atacggcacc gactggaatt caaaatatca agggcgagct ttacccatcg
     5761 tattacaaca aatctgccgg cgttaccaat gcaaccatga gcttcgataa ggtttcaaag
     5821 aagaaagcga ctgactgtac accagctggc gcaaagactg atattggcgt cagcaaaacg
     5881 accgacccaa ttaccaagca agtggttatt actgcaccgg atgggtatga tcctaattct
     5941 gacgatgacg tacactcatg tagcgacgca caaccaacgg tcagcatcgc ccaaagcggt
     6001 agctcactgg ttgtcaccgt acactctgga acgttcactg ttcaaggtgt aacattgacc
     6061 gagaatggta acacgatcgc gacacagagc ggcgcgggcc catggacatt taccgcaagc
     6121 caactcagca gtgttacaaa tggtacagtg acagcaacgg tcaccgatga aggctactat
     6181 actgcaacga atactgtaaa ctatagctca tcgagctgaa gtgataacag tggcggcaaa
     6241 catcaccact aagcaaatac cactccgtgt taggagtggt atttaattgt tgtgtagcta
     6301 gactctattc gtctttttgg ctatcaacaa gctgcaagag cgaatcttcg tgattcacgc
     6361 gtttacgagt ggcatgatag actgcgcgtg gcttttgttg gtttgcagat ttctgcgcgg
     6421 gctgattttg tgatagctgc ggcttttgtt gttttgactg ttgctggagt tgaggttccg
     6481 ctcgttttgg cttagtggtc tcgttactat cgttcctgcg actccggcct aatgattttg
     6541 tttttgccac tactattgcc ggtgcctcca ctttcttagg cgcctgtcgc tcgacgcgac
     6601 gcgcgaacat catcttgccc gcagcagtct gtagactgcg cactacctca acctgtactg
     6661 tttgcccaat atatttgtta gattgctcaa ctacaaccat tgtaccatca gataaatatc
     6721 cgacaccctg gtggctgtcc tgcccctttt gggtaagctc gatcatcatt tgctcacctg
     6781 gcaggcgttg catgcgcagg ctctgggcga gttcgttaat attgagcacc ttaatgccct
     6841 cgaccacggc aactttattc aaattataat caagcgtaca aattacagcc gaatgacgtt
     6901 tcgcgagggt taagaggcgt tcatcaacgc cctcggcggc tttgctacca tcctgaagaa
     6961 tctctacctc tatgccatcc atgccttgca gtgtcgatac gacatcgagt ccatggcgag
     7021 ctcgcgcacg cttgtctgcg tcagcctggt cggcgagaag ttgtagctca ccaattacgc
     7081 tacggggcac aacgagtgtg ccaccaataa atcccgtaga cgcaatgttt gtgatgcgac
     7141 catccatcag gaccgatgta tccactaaaa ttgcacgatc tttcgcttta attgttgatg
     7201 attttggaag cttagcgagc aaatacgata cttcgccaag aataataagc gttactgcta
     7261 gcaacactat atctcgtgtt tccatattat tccttactga ctatgcaaga tagtcgatga
     7321 gtgcggcacg aatatcgcgc acgccattta taaatgagtt ctttttgcca ggttgtactg
     7381 gcgcgaccgc ctgagtgaaa ccaagtttct tggcttcggc aactcgctta tccgcttggg
     7441 ctactgagcg gatctcgcca cccaggccca cttcgccgta gacaacgagt ccgtcgtcta
     7501 gcttgcgccc ggcagcagca ctcgcaatag ccatagctac tgcgagatcc gccgctggat
     7561 cggcaagttt gagacctccc acaacattga tatagatatc tttatcagat aagttcagct
     7621 tggtgcgttt ttctaacacc gcaatgagga ggtttaatcg atttaggtcg aatccgcttg
     7681 ccgttctctt aggatacccg aaattgcttt gattgacaag ggcctggatt tctactagaa
     7741 taggtcgatt tccttcaagc gtagcaagca ctactgatcc atcagcgttt tgacgttctg
     7801 cgagtagtgc ggcggacggg tttgaaacaa tcttaagccc gctttcgttc atctcgaata
     7861 ttgctgcctc gtttgttgag ccatagcgat ttttcactgc tcgaaccact ttaaatccgc
     7921 cgtaccggtc accttcaaac tgtagtacta catcaacgag gtgctcgagc acctttggcc
     7981 cagcaattga gccttctttg gtaacatggc cgactagtat tactgctgca cctgattctt
     8041 tggcggcacg aataataaca ttcgaagagt tggtaatctg gcttaccgta cctggagcgc
     8101 ttgtaatttc atcgaggctg agtgtttgaa tgctatcaat aatcactact ttgtacgcgc
     8161 cactacgaat tgtagcagtg atgtcatcag cacttgtgct tgatacgaat tgcaaatcat
     8221 cgctcgcaac tgcaccgaga cgtgcggcac gcaattttac ctggctcgcg ctttcctcgc
     8281 cacttgcgta gagcactggc tgcttttcgg caatctttgc tgccacctgc aacagtaagg
     8341 ttgacttgcc gatgcccggt tgaccggcta gcaataaaac cccacccggt agtatgccgc
     8401 cgcctagtac agtgtcaaga tcttcaaacc ccgtcgacat acgctgaatt gtctcttcgg
     8461 cactaattga gcgcattgac tgcggtgaca gtactttgcc actgtgagct ccgcgcgcga
     8521 ccgcagaact ccctacgggt tgcatgattt gctcgacgag gctattccac tcgccacagt
     8581 tctcacactt acccgtccat tttgggtaac ttgcgccaca atgctgacag ataaattgtg
     8641 atcgtgtttt tgccatattt agatactatt tggcgcctca acaccgttaa tgctctgatt
     8701 cagttgcttt gcacgtactg caagcttgtt gtactctgcc acatctgcat tataggtgtc
     8761 aatctgttga tttaacgcaa tttgccgatt cttcatttgc tcaatacgca ctgcaagtgc
     8821 ctggcgggct gttgtaaatg cgtattggga tgagaattcg ccgtttgttg cccactcatt
     8881 aaagctttcg atatccgaat tgagttgtgt taagtcggca ttatactgac tgctcgcttg
     8941 cttgatagcg gctgcttcat ctgtgatttt ctgctgtagt actgtagcct gctggttcac
     9001 ttcatcgaat actgctgtat agtgtgcatt cattgctacg atcgcagcgc gatccgtaaa
     9061 atagcgcgtg taatactgct ctagatcagt aggcaaatcc ttaatcgtcg tgccgataat
     9121 tgaatggagc tcattgagtt cttcgcccgg ttcagcttga gcatagtact gcatttcctg
     9181 cttgatatca gggttgtcct taatctttgc gtattcgata ttgagcattt tgttaacatt
     9241 ttcctgctca aatatgttca atcgcgcata tgcagcatgc agcatctcat gcgccgccgt
     9301 gacttcgagc gcaccgtcga gttctttacc ttcaatgttg tataaataga tctggtcgcg
     9361 aacgtaacac cccagtattg ctgctgtccg ttctgcactc ccgcagtcag tattgaaggt
     9421 gtctcgttct tcaatagctg gtgtagttgc ataaaatatt tgttgaccgc gcgccgttag
     9481 tgcaatacgg ctgttgacac ccacaatatt gctagttggg cgaaaatttg atgcgcggat
     9541 ggcatccacg gcgattggac catagaagta agcgcccgcc gcgattgcgg cgaacactga
     9601 taagctaagg agtccgccca gtattttatg cttcggacgt tgcggcggca actgactcgg
     9661 ttcgttgttc aatttgtact tcccctttag accaacctac ctctagtata gcgccttttt
     9721 catcactgcc ggctaaaagg cgctcggcga ttgcgtgttc tacctcatct tcaatcacac
     9781 ggcgcagtgg gcgcgcacca aattttgcgc tatagccctt atctattaac tgtcgttttg
     9841 cagctgcaga tattgcaata ccaagacctt tacggacaag tcgctggcgt agttcctcaa
     9901 tcagaagatc aaaaatctta ccaacctctt cgcgggttag tgccctgaag gtgacgatac
     9961 cgtcaaagcg gttaatgagc tctggacgca taaatttctc aagtgcctcc atagcataac
    10021 ttgcattacg atcgtggagc gtatcgagct ctctgttatg ctgcttcaat gagtggaatc
    10081 ccagctcaga ctctttcatc attttatcag cgccaaggtt gctggtaaga ataatgatgg
    10141 tattgcggaa acttacttcg cggccttttg catccgttaa cttgccctct tcaagtagtt
    10201 gaaggagtaa ttggaacaca tctgggtggg ctttctcaat ctcatcaaat aacaccacac
    10261 tatacggttg gcggcgaatc ttatcagtta gtttaccgcc atcttcgtag ccaacataac
    10321 ctgctggagc accgacgagc cgtgaagtat tgtgcttctc agcaaattcg ctcatatcaa
    10381 tcttaataag tgcctcttga ctaccaaaca cctcttttgc gagcaccttt gcaagctccg
    10441 tcttaccgac accagttggg cccataaaca cgaaacttcc tatcggacgc ttactgctcg
    10501 cgacaccact gcggctacgg cgaattgcgc gcgctacttt cgtgattgct tctttttggc
    10561 caatcagttg tttggacaag tgtttttcga gatggcggag catctttgcc tcgctggagc
    10621 gcactttttc gacgggaatg ttggtcataa cggcaatcgc gtgtgcgacg tcatcatcag
    10681 taagcgcgac agtattcttg ccctcctgcg cctgcttttc aacctcaatc ttttcagaaa
    10741 tctggctgac acgctgcttg tacagtgctg ctcgctcgta atcttcgtga ctaaccgctt
    10801 cttccatctt ttcattcaag gctttcagtt cttttgttag ttcgcgttgc ctgctcggtc
    10861 tatggccttg ctttacgcgg acacgcgccg cagcctcgtc gatcacatca atggctttgt
    10921 cgggcataaa tcgatcactc acgtagcgat cgctcatata tactgaagat tcaatcacct
    10981 catcggtgat cgcgacgcta tggtgctttt catagtatgc tttgaggcct cgcaaaattg
    11041 caattgtctc tttaagtgtc ggctctttca cgacaacggg ctggaagcga cgttcaagcg
    11101 cggcatcttt ttcgatgtgc ttacgatact catcaagcgt agtagcgcca attagatgta
    11161 gctcg
//
LOCUS       DATIIX010000052         6607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_401365, whole genome shotgun sequence.
ACCESSION   DATIIX010000052 DATIIX010000000
VERSION     DATIIX010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6607
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401365"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VLF64_01530"
     CDS             complement(<1..754)
                     /locus_tag="VLF64_01530"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: protein motif:HMM:NF015370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - acyl-[acyl-carrier-protein]
                     desaturase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HSW90657.1"
                     /translation="MSERPNLEQPSSRELWLPSSVRRRGQEAIIRSGLVVTGDEPHQL
                     NILRELEPVVADELNRHHRMRNDWTPIDYMPIDQQTGQILHRVENSEEVPLLSPEAQA
                     AMIVNLLTEDNLPSYHRTIAENFGLDGPWGQWTHQWTAEEDAHAYAIRMFLDLTRAVD
                     PDENEAMRMQQMRQGYTVEKDPLHTLAYVTFQELATRVAHRQTGIASKNDTADKMLQR
                     IAKDENLHMLFYRNLASAAIDVAPNQMMQAIRD"
     gene            823..2526
                     /locus_tag="VLF64_01535"
     CDS             823..2526
                     /locus_tag="VLF64_01535"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HSW90658.1"
                     /translation="MSSTFALPSILRYKRQDFRHDVMAALVLTAITIPQSLAFAVVVG
                     LPPVTGLYTALFAPVVFALFASTRRLVVGADSATAALLASSATLVAQVGTAGYTSAIG
                     TLGALTALILLIVAVLRLGFLSDLVSRPVIVGFLAGVGVQLIITSVPVMLGAHMVLGT
                     VWDSIAGLLRFPAVNWMTITITVLVVGILLLVRGSRVPGELIGIIVAVIFAAVFQVER
                     FDVALVGDLPSHLPVFVLPAFSLTTIWTLLPAAAMVAAIILAQSSAVVRRIAEEHDEK
                     IRVNQDLFALGMANVASAITQGFAVNGSPPRTMAVDKAGGRTQLTEILSSGLVGFILV
                     FGVGLLAYLPQAALSSIICLLGVHLIRLGELRYLWRVHRTEFAVAIIALVGTAFVGVL
                     QGVLIAVIVSLSERVIRQYHPKDEILLRDGELSDWARERLSSRVQQSSQLEGVLVYSF
                     DGSLFFENITYFVSRLKRAIKGAAKPVKTVVIDAGSIDSIDYTAVEAIKHSYRKLSED
                     NIALRFAHVSPTLHKQLVEFGVVELLGNRHIFSTLTEAITGKTPSHDEESKEGVVAKG
                     H"
     gene            complement(2523..2996)
                     /locus_tag="VLF64_01540"
     CDS             complement(2523..2996)
                     /locus_tag="VLF64_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90659.1"
                     /translation="MAFKKLHAAAPIAAHKKLVIDSTWAVDPLISQSDIFTCAQHALL
                     GATDFTLQVLQRSIPQVPATAHRPISTGNTSDIAWATWHHGKDFYTYWCGDVTAVLHH
                     ASLTDTERETVLLRARKAEGQGMQTFATGAKLTHQAPRAVEFDIEHIGLIFACSI"
     gene            3060..3311
                     /locus_tag="VLF64_01545"
     CDS             3060..3311
                     /locus_tag="VLF64_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90660.1"
                     /translation="MAKSSSAQKKSIAQAEFTTKEMVVKSSKPSKKTAPKKRSMVKKP
                     VEGAQQKVDYYPNRMTLAISALAGTLIVLLALIAVLGQR"
     gene            3359..4510
                     /locus_tag="VLF64_01550"
     CDS             3359..4510
                     /locus_tag="VLF64_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSW90661.1"
                     /translation="MKVRIEIDTKTFVRFWLVVIGFGIGILALYSARTALVIIGTAFF
                     LALALNGPVSRLSRHLPDRSRTLSTAIAFFAVVLFLGAVIFLVIPPVVQQTVKFIDNA
                     PTLVQTVSDQWHGVGDLVAKYHIQPQVDQAVSAIRDNAEHWASGFGRNFLSGLGSAMS
                     LFAASLLVLVLTFLMLIEGPTWMERIWSVYKNDRRVKSHKRLVHRMQAVVSGYVSGQL
                     SVSGIDATAAGITVFILSRFFSEVPANLALPTVAFCFIFSLIPLFGATLAGIIVTTLL
                     AMNSVPAAIVFAIYFIIYQQLENNVISPRIQSRRIELSPLAVLTAVTIGLYVFGIAGG
                     IISIPIAGCIKVLVENYFEEHGEDIDEPVKEEEPKHRKISLKFFGNSED"
     gene            complement(4507..5970)
                     /gene="cysS"
                     /locus_tag="VLF64_01555"
     CDS             complement(4507..5970)
                     /gene="cysS"
                     /locus_tag="VLF64_01555"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HSW90662.1"
                     /translation="MPLKLHNTLSRKLETFAPINSDKVMLYTCGPTVYDHPHVGNWAS
                     FIYWDILVRTLIASGYRVERVMNITDVGHLTSDADEGEDKLEKGARREGKNAWDIAEF
                     YAEAFIDGMETLGLLPPEHLVRATDFIPQQLDLIRELKEKGFTYQTTDGIYFDTAKFP
                     TYADFAHLDLAAQKAGARVEANDEKRQAWDFALWKFTEPGIVRDMEWETPADLLDLPP
                     REAEPIMGFPGWHLECSAMAMSILGSTIDIHTGGIDHIPVHHTNEIAQSEAASGVKFA
                     NYWLHNNHLKIEGGKISKSLGNGYTLQHLTDRGLSPMDFRLFVLQGQYTNEGNFTFEN
                     IEAAKNRLSNWLGYALLRHQIHDHGTHAVRADDIPVPSTAASGALREALQNNLNTPEA
                     LRIIDETFAALDNKPLNAIDRQNLIDFLETIDELLGLQIMTSTPDIDDEAKRTILERR
                     RARDNKDWKTSDELRDRLAAKGIFVRDTTYDTIWSRS"
     gene            complement(5936..>6607)
                     /locus_tag="VLF64_01560"
     CDS             complement(5936..>6607)
                     /locus_tag="VLF64_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90663.1"
                     /translation="QNDLSFDHEFVLSPTAAATGELIYNLAQTAGWEVTPAAASALYS
                     SIASDTLGLTTESASIDTYRVVAEVVAHGAKPFEIESARREFMKKPADILTYKGELIE
                     RIEYSLDGKLATVHIPWEDIQEYSDRYNPSVLVLDEMRLVDTVEVACAIKTYPDGKIT
                     GKLRCNLPVANQIAGYFGGGGHSYAAGFKVHEQYETIMQELVTVTDKALKDYHATQAS
                     QHIIA"
BASE COUNT         1427 a         1429 c         1763 g         1988 t
ORIGIN      
        1 catcccggat tgcttgcatc atttgatttg gtgctacatc gattgccgcg cttgcaagat
       61 ttcgataaaa caacatatgg aggttttcat cctttgcaat acgctgcagc attttatcgg
      121 cagtatcgtt tttacttgct atgcccgtct gccggtgagc tacgcgggtt gcaagctctt
      181 ggaatgttac atatgcaagc gtgtgaagcg gatctttttc gactgtgtac ccttgcctca
      241 tttgttgcat gcgcattgct tcgttttcgt cggggtcaac ggcacgagta aggtcaagga
      301 acatgcgaat tgcataggcg tgagcatctt cttcggcggt ccattgatgt gtccattgcc
      361 cccacggccc atcgaggccg aagttctcgg caattgtgcg atgataagac ggtaggttat
      421 cttcggttag aagattaacg atcattgcgg cttgggcctc gggggataat aaaggaactt
      481 cttcagagtt ttctactcgg tgaaggattt gtccagtctg ttgatcaatc ggcatgtaat
      541 caattggagt ccagtcgtta cgcatacgat ggtggcggtt tagttcatct gctacaactg
      601 gctcgagctc gcgtaagata ttaagctgat gaggctcatc gcctgttacg acaagaccac
      661 ttctgataat cgcttcttga ccgcgacgtc ttacgctgct tggtaaccat agctcgcgtg
      721 aggatggctg ctcgagatta ggtctttcac tcatatgcct atcatagcat aagcactgca
      781 aacgtcgttg acgcgcactg aggttgctac tactatatag ccatgagtag tacgtttgcc
      841 cttccgagta ttttgcgcta taagcgacaa gattttcggc atgatgtcat ggcggcgctc
      901 gtactgacag cgattacaat accgcagtcg cttgcgtttg cggtggttgt cgggttgcct
      961 cccgtaacgg ggctttatac agcccttttt gcgccagttg tatttgcgct ttttgcaagc
     1021 acacgccgat tggtagttgg tgccgattcg gctactgcgg cgctattagc ctcgagcgcg
     1081 acgttagttg cgcaggtggg aacggccggc tatacgagtg ctattggtac acttggtgcc
     1141 ttgacggcgc ttatcttgct cattgttgcc gtgctacggc ttgggttttt atcggatctg
     1201 gtatcgcgac cagtgattgt cggattctta gccggtgttg gcgtgcaact tatcattacg
     1261 agcgttcctg taatgcttgg tgcgcatatg gtattaggaa cagtctggga cagcattgct
     1321 gggttgctgc gatttcccgc cgtgaactgg atgacgataa ctataacggt ccttgttgtc
     1381 ggtattttac tattagtgcg tggttcgcgg gttcccggcg agctaattgg gattattgtt
     1441 gcggtgatat ttgccgcggt attccaggtt gaacgttttg atgttgctct tgttggtgat
     1501 ttgccaagtc atctgccagt gttcgtactg cctgcatttt cgttaacaac tatctggaca
     1561 ctattaccgg ctgctgcgat ggtggcagcg attattttgg cgcagagttc agcggtagtc
     1621 cggcgtattg ctgaggagca tgatgaaaag atccgggtga atcaagattt atttgcgctt
     1681 ggtatggcga atgtggcgtc agcaattaca cagggatttg ctgtaaacgg cagtccaccg
     1741 cgcactatgg ctgtcgataa agcgggagga cgtacacaac ttacggagat cttgagcagt
     1801 ggactggtgg gttttatcct agtatttggc gttggtctgc ttgcatattt gccacaagcg
     1861 gcgctatcgt cgataatttg tctactcggt gtgcatttaa tccggcttgg cgagcttcgt
     1921 tatttgtggc gtgtgcatcg tactgaattt gcggtagcaa ttattgcatt ggtgggtacg
     1981 gcgtttgtcg gggtgttgca gggcgtgctt attgctgtta ttgtatcgct ttcggagcgt
     2041 gttattcgtc agtatcatcc gaaagatgaa attttgctgc gcgatggtga gttgtcggac
     2101 tgggcgcgag aacgactcag cagtcgtgtg caacagagta gccaacttga gggagtgctc
     2161 gtatatagtt ttgatggttc tctgtttttt gagaatatta cctactttgt gtcacggctc
     2221 aaacgcgcga ttaagggtgc tgcaaagcca gtaaaaacgg ttgttattga tgcgggcagt
     2281 atcgatagta ttgactacac ggccgttgaa gcgattaaac actcgtaccg caagctgagt
     2341 gaagataata ttgcgcttcg ttttgcgcat gtgtcaccaa ccctacataa gcaactggtg
     2401 gaatttggtg tcgtggagct actcgggaac aggcatattt tctccacatt gacagaggcg
     2461 attacgggta agacgcctag tcatgacgaa gaatcaaaag agggagtggt tgctaagggg
     2521 cactagatac tacaagcaaa tatgaggccg atgtgttcta tatcaaactc tactgcgcgt
     2581 ggagcttggt gtgtaagttt tgcgcctgtt gcgaacgttt gcattccctg tccttctgcc
     2641 ttgcgggcgc ggagtaatac cgtttcacgt tcggtgtcgg tgaggctggc atggtgcagt
     2701 actgccgtga cgtctccgca ccagtatgtg tagaaatctt tgccgtggtg ccaggttgcc
     2761 cacgcgatat ccgatgtatt gccggtagat atgggtctat gtgcggtagc tggaacttgt
     2821 gggatgctac gttgtaagac ctgtagcgta aagtccgttg cgcctaaaag agcatgctgg
     2881 gcacacgtaa atatgtcact ttggctaatg agcgggtcaa cagcccatgt gctatcgata
     2941 acaagtttct tatgggctgc aatgggggct gcggcgtgta actttttgaa tgccatatgc
     3001 gtaaagtata gcataagcaa gttgtgcctc gcggtgcttt ctcgtatact atagctacta
     3061 tggctaaatc tagttccgcg cagaaaaaat caatagcaca ggccgagttt accactaaag
     3121 agatggtagt gaaatcaagt aagccatcta aaaaaacagc tccaaaaaag cgctcaatgg
     3181 taaaaaagcc ggtggagggt gcccaacaaa aagtagatta ctacccgaat cgcatgacac
     3241 ttgcaatatc ggcgctggct ggtacactta ttgttcttct tgctcttatt gcggtgcttg
     3301 gtcaacgcta aacctttaag gtatcccaag aaaggctttt atgcggtata ctgacaacat
     3361 gaaagtacgt attgaaattg acacgaagac ttttgttcgg ttttggcttg ttgttattgg
     3421 attcggaatt ggtattctcg cgctctatag tgcacgtact gctcttgtta ttattggtac
     3481 tgcctttttc ttggcacttg cgctgaatgg tcctgttagt cgcctttcac gccacttgcc
     3541 tgatcgtagt cgtacacttt cgacggcgat tgcttttttt gcggttgttc tctttctcgg
     3601 cgccgttatt ttcttggtga ttcctccagt tgttcagcag acggttaagt tcattgataa
     3661 tgcaccgaca ctcgtgcaga ccgtgagcga tcagtggcat ggtgttggtg atttggttgc
     3721 taagtatcac attcagccac aggtagatca ggcggtttct gcaatccgcg ataatgccga
     3781 acattgggca tctggctttg gccgtaattt cctctctggg cttggctctg caatgtcgct
     3841 ctttgccgca agtcttctgg tgctcgtgct aaccttcctg atgcttattg aaggtccgac
     3901 ttggatggag cggatctgga gcgtttataa aaacgaccgt cgggtgaagt ctcacaagcg
     3961 tcttgtacac cgtatgcagg cggttgtctc cggctatgta agcggacagc tatctgtatc
     4021 gggtattgat gctacggctg ctggtattac ggtgtttatt ctctctcgtt tctttagcga
     4081 agtgccagca aacctggcat tgccaacggt agcattttgc tttattttct ctctcatacc
     4141 actctttggt gcaactcttg ctggtattat tgttacgact ttactcgcta tgaatagtgt
     4201 gccggcagca attgtattcg cgatttactt tattatttac caacagcttg agaataatgt
     4261 tatctcacca cgtattcaat cgagacgtat tgaactgagc ccgcttgccg tactcaccgc
     4321 tgtaacaatt ggattgtatg tgtttggtat tgctggtggt attatttcaa tcccaatcgc
     4381 tggctgtatt aaagtacttg tcgagaacta ctttgaggag catggtgaag atattgatga
     4441 gccagtcaaa gaagaagagc caaagcatag aaaaatttca ctcaaattct ttggtaactc
     4501 agaagattaa cttcgtgacc aaattgtatc gtacgtagta tcgcgtacga agatcccttt
     4561 cgcagcgagt ctgtcgcgta gttcgtctga ggtcttccag tccttgttgt cacgtgcgcg
     4621 acgacgctca aggatggtgc gctttgcttc atcatcaatg tctggggtcg aggtcatgat
     4681 ttgtaatcca agtaactcgt cgattgtttc aaggaaatca atcaggtttt gtctgtcgat
     4741 tgcattgagt ggcttattgt cgagtgcagc gaatgtttca tcgattatac gaagtgcttc
     4801 tggcgtgttg aggttgtttt gcagcgcctc acgtagtgcg ccacttgctg cagtggatgg
     4861 taccggaata tcatcagctc ttactgcatg cgtgccatgg tcgtggattt gatggcgtaa
     4921 aagcgcatag ccaagccagt tactaagccg atttttggct gcttcgatat tctcgaatgt
     4981 gaaattgccc tcgttggtat attgcccttg caggacgaat agcctgaagt ccattggcga
     5041 gagtcctctg tctgtgaggt gttgcagtgt gtagccgttg ccgagacttt tgctgatctt
     5101 gccaccttca attttaaggt gattattgtg gagccagtag ttggcgaatt ttacaccgct
     5161 tgcggcttca ctttgggcga tctcattagt gtggtgtact ggaatgtgat cgatgccacc
     5221 ggtgtgaata tcgattgttg agccaagaat gctcatagcc atcgccgaac actcaagatg
     5281 ccagcccgga aatcccataa ttggttcagc ttcacgtggc ggcaggtcaa gcaagtccgc
     5341 aggtgtctcc cattccatgt cacgtacaat gcctggctca gtgaatttcc ataatgcgaa
     5401 atcccatgct tggcgttttt cgtcgttcgc ttcaactcgt gcgccggctt tttgtgcagc
     5461 gaggtcgagg tgcgcgaaat ctgcgtaggt tgggaatttg gcggtatcaa aatagatacc
     5521 gtcggttgtt tggtaggtga aacctttttc tttcaactca cgtattaaat cgagttgttg
     5581 tggaatgaag tcagttgcac ggacgagatg ctctggtggc agcagtccaa gtgtttccat
     5641 accgtctatg aacgcttcgg cataaaattc ggcaatatcc caggcatttt tgccctcgcg
     5701 gcgagcgccc ttttcgagtt tgtcctcgcc ttcgtcggca tcacttgtta agtggccgac
     5761 atcagtgata ttcatgacgc gttcgacgcg gtatccactt gcaatgagtg tacgtacaag
     5821 gatgtcccag taaatgaatg atgcccagtt gccaacatgc ggatggtcgt agacggttgg
     5881 accgcaggta tagagcatta ccttgtcgct gttgattggg gcgaatgttt caagtttacg
     5941 cgataatgtg ttgtgaagct tgagtggcat gataatcctt tagagctttg tcggttactg
     6001 tgacgagctc ttgcataatg gtttcgtact gttcgtgaac cttgaagcct gcagcatagc
     6061 tatgcccgcc cccaccgaag tagccagcga tttgatttgc tacaggcagg ttacaccgca
     6121 gtttgccagt gatcttaccg tctgggtagg ttttgatagc acatgctacc tcgacggtgt
     6181 cgacgaggcg catttcatca agtacgagta cgcttggatt ataacgatct gagtattctt
     6241 ggatgtcttc ccatgggatg tgaacagtgg ctagcttgcc atcaagtgag tattcaattc
     6301 gttcgatgag ctcacctttg taggtcagga tatcggctgg ttttttcatg aattcgcggc
     6361 gtgctgattc gatttcgaat ggtttggcac catgggcaac tacttcagct acaacgcggt
     6421 aagtgtcaat gctggcagat tcggtggtga gaccgagtgt atcagacgca attgaactgt
     6481 agagtgcaga tgcagccgcc ggagttacct cccagcctgc ggtttgagca agattgtaaa
     6541 taagttctcc cgttgcagcg gccgttgggc tgagcacaaa ttcgtgatcg aacgataggt
     6601 cattttg
//
LOCUS       DATIIX010000053         2930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_291265, whole genome shotgun sequence.
ACCESSION   DATIIX010000053 DATIIX010000000
VERSION     DATIIX010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291265"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VLF64_01565"
     CDS             complement(<1..349)
                     /locus_tag="VLF64_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90664.1"
                     /translation="MLPILLSITTFFSTLAGGLTALRFRDKLHRFLGYTAGVLLGVVA
                     FDLLPEIIGLVEQTKVETIWPMLALVVGFMAFHIVEKSILIHESQEHEYEVHKHPNVG
                     VASALPLAGHSFLD"
     gene            1169..1492
                     /locus_tag="VLF64_01570"
     CDS             1169..1492
                     /locus_tag="VLF64_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90665.1"
                     /translation="MQSLSPKLPLAVARSTLLHAPLRRKEIFVVPGNITSPMSAGCNT
                     LIKQGAHVVTDANDITELQAGLRDGDEIQRKTGLSSTDFNTSLTILEMQGIIRALGAN
                     QWTLL"
     gene            1585..2211
                     /locus_tag="VLF64_01575"
     CDS             1585..2211
                     /locus_tag="VLF64_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90666.1"
                     /translation="MKLLRRLSVLAVIVATIVAIVVLTSHNSSNPAAGTQSDPHVGTS
                     RYPAPTAANHTPPSATVAVANNPNAVPALANVNDKCLWAARGNNPPSLQDDGQGSQSY
                     ADMPSAVSDNTLRCAIARAKAAGYKVEINSVTCDEPVIQLLDESNNKLWSFKPFCMYI
                     GRGGPLCKIPLVVTDYQGNFLRQSEPDLKRLQNFRADSVLNESAYADC"
     gene            2364..>2930
                     /locus_tag="VLF64_01580"
     CDS             2364..>2930
                     /locus_tag="VLF64_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HSW90667.1"
                     /translation="MSKNLVIVESPAKAKTIEKYLGSDFTVLSSVGHIRSIAKKTKDG
                     TPPIDVKNGFKTTYEVDSEKKKIITELKKQVKAVGKENVWLATDEDREGEAIAWHLCE
                     VLGLDPLTTKRIVFHEITKDAITHAVANPRTIDMKLVEAQQARQILDRIVGFELSPVV
                     WQKVPGGKSAGRVQSPAVRLLVEREREIR"
BASE COUNT          829 a          798 c          694 g          609 t
ORIGIN      
        1 catctaaaaa gctatggcct gccagcggga gcgccgatgc cacacccaca ttcggatgct
       61 tatgcacttc atactcatgc tcttgcgatt catggataag aatcgacttc tcaacaatat
      121 gaaaagccat gaatcctacc acaagcgcca gcatcggcca aatcgtctca accttcgtct
      181 gctcaactaa tccaataatt tcaggcaaca aatcaaatgc tacgacaccc aaaagtacgc
      241 ccgctgtata gcctaaaaaa cggtgtagtt tgtcacgaaa acgcagggct gtcaaaccac
      301 ctgcaagagt tgagaaaaat gttgtaatag aaagaagaat cggaagcatt agatttattg
      361 tacctgatta tttcagacaa ccaaaaagcg ccaggtaagg gtgggcatca cctggcgcca
      421 tttggaccct tcatcccacc ctgatcgggc atagcgtgac ccacctcaca acatatggag
      481 gtccgccgga taaaggggtt agtatacgca ctaaagcttg tctaacggtg atgtagtgga
      541 gggtagaata ctgcatcaca ccatagtgcg caacctaaca taagtagaat ttcctgttag
      601 ggaaatcctg ttagaatagg gggtataagg tacttgatgt gcctctcggt catcaatcgt
      661 atctcatatt agcatacctt ttgacttatg tcaacactta gcgcaataaa atcatatgaa
      721 tatcaatagc acataaccag ataagcataa gtttctacag aggttagatc agattgacaa
      781 acctgtggag aaagtatttt acagcgattg tctgtcccac acgcatacct accgtagccg
      841 ttattggcac tcgtaagcca actacatatg gtaaagagac tacgtacaaa ttagcctccg
      901 aactcgctag aaaagacgtg gtagtggtaa gcgaattagc actcggagtc ggacagtatc
      961 gcccatgcag ttgcatgcga cgtaggtgga atcgcccttg cggtgctacc ctcaggttta
     1021 ccacatattt atccatcaag tcaccgcagt ttagccgaac gcatcctaaa atctggtggt
     1081 gtcctcataa gcgagtgcaa agaaaccgcc acgaatgcct accaaagcaa ctttttagag
     1141 cgcaattgca tcgtttcagg gcttgccgat gcaatcctta tcaccgaagc tgccgctcgc
     1201 agtggcacgc tcaacactac tgcacgcacc cttgcgccgc aaggaaatct ttgtagtgcc
     1261 gggaaacatt acgagcccaa tgagcgccgg gtgtaataca cttatcaagc agggcgcgca
     1321 tgtcgtgacc gatgcaaatg atattaccga gctgcaagca ggcctgcgtg atggcgacga
     1381 gatccaacgc aaaacagggc tgtcatctac cgatttcaac accagcctca caatactaga
     1441 gatgcaggga ataatccgcg cactcggagc gaatcaatgg acgctcctct agagaccatt
     1501 gacaaatata agcattattg atataatata ctcacaactc ggcatccggc cgaccgcacc
     1561 atctacccgt taggaatagc tcagatgaag cttttgaggc gcctcagtgt gctcgccgtc
     1621 atagtcgcca caatcgtggc gatcgtcgtg ctcacgagcc acaactcgtc gaaccccgcc
     1681 gcgggaaccc agagcgaccc ccacgtcggt acctcccggt acccggcgcc gacagccgca
     1741 aaccacactc cgccatccgc gaccgtcgcg gtagccaaca acccgaacgc cgtcccagcg
     1801 ctcgccaacg tcaacgacaa gtgtttgtgg gcggcccgcg gcaacaaccc gccgtccctc
     1861 caggacgacg gccagggctc gcagagctac gccgacatgc ccagcgccgt cagcgacaac
     1921 acactgaggt gcgccatcgc gcgcgccaag gctgcgggct acaaggtcga gatcaactcg
     1981 gtcacgtgcg acgagcctgt catccaactg ctcgacgaga gcaacaacaa gctctggtca
     2041 ttcaagccgt tctgcatgta catcggccgc ggcggtccgc tttgcaagat cccgctggtc
     2101 gtcaccgact accaggggaa cttcctgcgg cagtcggagc cagacctgaa gcggctgcag
     2161 aacttccgtg cggacagtgt gctcaacgag agtgcctacg ccgactgctg agctccaacg
     2221 ggcatcccgc cccggaaagt gtcccattag ggggtgccgc cggggcgctt ttcgtgtagc
     2281 acttgcattt tactcgtaag cacgttacta taagccactg taatttggca atgtatgcta
     2341 ataatatata gtgaatagag cttatgagta aaaacctagt aatcgttgag tccccagcaa
     2401 aagcaaaaac tatcgagaaa tacctcggga gcgattttac ggtactcagt tccgtgggcc
     2461 acatccgttc aattgcgaaa aaaactaaag acggtacacc gccaatcgat gtaaagaatg
     2521 gatttaaaac aacctatgaa gtcgacagcg aaaagaaaaa gattattacc gaacttaaaa
     2581 agcaagtaaa agctgttggt aaagaaaatg tctggctcgc aaccgatgaa gaccgcgaag
     2641 gagaagctat tgcatggcac ctttgcgagg tactcggcct tgacccactc acgacaaaac
     2701 gaattgtgtt ccatgagatc acgaaagatg caattacgca tgctgtcgcg aacccacgca
     2761 ccattgatat gaaactagtc gaagcccagc aagcccgcca aatcttggac cgtatcgtag
     2821 gcttcgaact aagtccagtg gtctggcaaa aagtacctgg cggcaagtct gcaggtcgcg
     2881 tacaaagccc tgcagtacgg ctactcgtcg agcgtgaacg tgaaattcgt
//
LOCUS       DATIIX010000054         6046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_540210, whole genome shotgun sequence.
ACCESSION   DATIIX010000054 DATIIX010000000
VERSION     DATIIX010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540210"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VLF64_01585"
     CDS             <1..915
                     /locus_tag="VLF64_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HSW90668.1"
                     /translation="DVTAIEHYKSVLAACKERGIEPIITLFHFTLPVWFVEMGGFERA
                     ANVRLFVDYAMRVLEEFGMSVKYVITMNEPEIYVRHGYIEGLWPPQLMNKRLGKTVLA
                     NLVKAHNQIADKLHAASRRYKVSIAKDYSYIYPGDDAWLTVRAASYKHRQHNEAFLNK
                     VIKTCDFIGINYYFSERVYGYRVHNPDLRVNDVGWDMQPQYLARVLEELHEKYDKPIF
                     ITENGLADGVDKDRIWWLTQTILALQVAIKKGVPVMGYLYSNLTDGFEWEKGMWPQFG
                     LFAVNPKTMERTPRQSARWLAKTLKKLR"
     gene            921..1913
                     /locus_tag="VLF64_01590"
     CDS             921..1913
                     /locus_tag="VLF64_01590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HSW90669.1"
                     /translation="MSKHGANIVVIGGGTGSFTLLQGLKSYTSNITALVNMADDGGST
                     GQLRDELGVLPPGDVRQCLVALSAAPQVRDLFNYRFEEGSLKGHAFGNLFLTALEKMS
                     GNFAEAVELASLVLKITGRVEPITLSNVRLCAEGADGKPIIGECNIGEAALTPRPHIW
                     LEPEADANPAAVRAIMDADMVVIAPGNLYGSLAPALVIDGVQRALRRTKAKCVYVANL
                     VTKPGPTDGFTVKDFADEIERLVGSKFLDYVVFNNDTSSDELVERYARDGEYAVAFNI
                     PELKKQHYQFRAASILAKHAWDGAQSSDPLAASRSFIRHDSDAVARQLMRIYFT"
     gene            1913..2515
                     /locus_tag="VLF64_01595"
     CDS             1913..2515
                     /locus_tag="VLF64_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90670.1"
                     /translation="MKSIFYLDLDRTLFRTERVGEFFPALEQLYPQNTALHDSYALRE
                     QYFVFPQHKDGDEKTYYYDVARQLRDAGLDIEQAFSALRTELGDGRFEHEGVRDFVRA
                     LQVHGVVKILTYGEDIYQRFKVSLCPSLAGLEVITTLEPKAKYLNKHSVAGDWVVDDK
                     IIDDLTTGIQTVHIQYDMAKPADVYSLTEATEHIVRGISF"
     gene            2573..2707
                     /locus_tag="VLF64_01600"
     CDS             2573..2707
                     /locus_tag="VLF64_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90671.1"
                     /translation="MKSAVPTTEVKVQEKKPYSPKMAVVNIVMFSSLFLVWLVLQLQK
                     "
     gene            3485..4375
                     /gene="rsmH"
                     /locus_tag="VLF64_01605"
     CDS             3485..4375
                     /gene="rsmH"
                     /locus_tag="VLF64_01605"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HSW90672.1"
                     /translation="MSIKEHPPLHVPVLLESVIALLQPQLGENYLDLTAGYGGHARVV
                     LEKTDNYIESALVDRDDFAIAHLGEFSDKGTRLLHTDYLSAAKQLVEEGKQFDIILID
                     LGVSSPQLDMQARGFSFNRDGPLDMRMDNRQSLTAETIVNTAKRNELTRIIREYGEES
                     PGTAKRYAEAIIAARPIQTTGQLADVIKQSHVGKWQKTHPATRTFQAIRIQVNDELHQ
                     VREVMPLLPRLLKKGGRVGVISFHSLEDRIVKRYFAEQTRSGYEAELEIVTKRPIDGS
                     IQDVHNPRSRSAKLRVAVKK"
     gene            4544..4894
                     /locus_tag="VLF64_01610"
     CDS             4544..4894
                     /locus_tag="VLF64_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90673.1"
                     /translation="MSYNRTVQFNSRRQTNWQRNQNTVAFASAVKLGPISHTVLVALM
                     ITVLGLIYLTQATRVSGYDYAANAISDKISSLTEQKDNLEVENARLTALQTVKNSNVA
                     KAMTTPTDVQSVSE"
     gene            4914..>6046
                     /locus_tag="VLF64_01615"
     CDS             4914..>6046
                     /locus_tag="VLF64_01615"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HSW90674.1"
                     /translation="MELHFSQGSRAKKIAVVTLAAMAIFIVRLFYIQVVQHEKYVAQA
                     DQEQVKQFTLHAKRGEIYTMDGSTPAKLVMNQTVYTVWADPTEVTDKNAVISALNSIA
                     GGNVRDNFAQYLDMSHSRYQVLATKVTMQQAEMLKKKRLAGIGFDGVSQRVYPEGQLA
                     SQVLGFVDANGEGKYGFEQANDAQLKGKDGLLKTVTDIRDVPLTVSDKNIRQPAVNGQ
                     NMVLTIDRNIQAETEQALADGVAKSGADHASAIVMDPQTGKVLAMANVPTYDPSQLNK
                     VTDVAQLNNDIVSAPYEPGSDIKTFTVATGVDKGVITPGSTYNNTGQIKVDDITINNA
                     TKTAAVTGNITMQTALNWSLNTGMVTIAERLGDGNYITRQARDT"
BASE COUNT         1739 a         1293 c         1497 g         1517 t
ORIGIN      
        1 gatgttactg caatcgaaca ttacaagtct gtgctcgctg cttgtaagga gcggggtatt
       61 gagccgatta taaccctctt tcactttaca ttgcctgttt ggtttgttga gatgggcggg
      121 tttgaaagag cggcaaatgt acggcttttc gtggattatg cgatgcgcgt tttggaagag
      181 tttggtatga gcgtgaaata cgtaattacc atgaacgagc cagaaatata tgtacgtcac
      241 ggctatatag agggtttatg gccgccgcag ctaatgaata agcgtcttgg taaaacggtg
      301 cttgctaatc tcgtcaaagc gcataatcag attgccgata agctccatgc tgctagccgg
      361 cgctataaag tttcgattgc taaggattat agctatattt acccaggcga tgatgcttgg
      421 ctgaccgtgc gtgcggcaag ctataagcat cgccagcata atgaagcgtt cctgaacaaa
      481 gtcattaaga cttgtgattt tattggcatt aattattatt ttagcgaacg tgtgtatggc
      541 tatcgtgtgc ataatcctga tctacgagtc aatgacgtcg gctgggatat gcagccacaa
      601 tatttggcgc gcgtgcttga ggagctccat gaaaaatacg ataaaccgat ttttattacc
      661 gaaaatggac ttgcagacgg cgttgacaaa gaccggatat ggtggctgac gcagactata
      721 ttagcgttac aagtggcaat taaaaaaggc gtacctgtga tggggtattt gtacagcaac
      781 cttaccgatg gatttgagtg ggagaaaggc atgtggccgc aatttggact atttgccgtg
      841 aatcctaaga caatggagcg cacgccaagg caaagtgcac gatggctcgc taagacactc
      901 aagaagctac gatgataaat atgagtaagc acggcgcaaa tattgttgta atcggtggtg
      961 gaacagggag ctttacacta ctgcagggac ttaagagcta cactagtaat attacggcgc
     1021 ttgtgaatat ggccgatgac ggtggctcaa cggggcagct acgtgatgag ctgggcgtgt
     1081 tgccgcctgg agatgtacga caatgtttag tggcacttag cgcggcacct caggtgcgcg
     1141 atttgtttaa ttaccggttt gaagaaggaa gtttgaaggg gcacgccttc gggaatttgt
     1201 ttttaacagc acttgaaaag atgtcgggca attttgctga ggcagtggag cttgcgagct
     1261 tggttctgaa gattactggt cgggttgagc caatcaccct gagcaatgta aggctttgcg
     1321 cagagggtgc tgacggtaag ccgatcattg gcgaatgtaa catcggggag gccgcattaa
     1381 cgccacgtcc tcatatctgg cttgagcctg aggcggatgc taaccctgca gccgtgcgcg
     1441 caattatgga tgcagatatg gtggtgatcg cgccaggcaa tctgtatggc agccttgcgc
     1501 ctgcacttgt gattgacggg gttcaacgtg ccctcagacg tacgaaagcg aaatgcgtgt
     1561 atgtggcgaa tcttgtcaca aaaccagggc caactgatgg attcaccgta aaagattttg
     1621 ctgatgaaat tgaacgactt gtaggtagca aatttcttga ctatgtggtc tttaataacg
     1681 atacgtcgtc ggatgagtta gtagagcgct atgctcgcga cggtgaatat gcggtggcgt
     1741 ttaatattcc tgaacttaaa aaacaacatt atcagtttcg tgcggcgtca attttggcga
     1801 agcacgcttg ggatggcgcg caatcaagcg atccgcttgc ggcttcacga agttttatcc
     1861 gccatgattc ggatgctgtt gcacgtcaat taatgcgtat ttactttacc tagtgaagtc
     1921 gatattttat cttgatttag atcgcacgct cttccgtact gagcgagtcg gagaattttt
     1981 ccccgcactt gagcaactct acccacaaaa cacagcttta catgatagct atgcgctgcg
     2041 tgagcaatat tttgtatttc cgcagcataa agacggtgat gagaaaacgt attactatga
     2101 tgttgcgaga caattgcgtg acgctggact tgatattgag caagcttttt cggcgctgcg
     2161 caccgaactt ggtgacggcc gatttgagca tgaaggtgtt cgcgattttg tgcgggcgct
     2221 gcaagtgcac ggcgtagtca agattcttac ttatggtgag gatatttatc agagattcaa
     2281 ggtgtcgcta tgcccgtcgc ttgcgggtct ggaggtaatt acgacgcttg agccaaaggc
     2341 gaaatatctc aataagcact ctgtggcagg ggactgggtt gttgacgata aaattataga
     2401 tgacctgaca acaggtatcc aaactgttca cattcagtac gacatggcga aacccgcaga
     2461 tgtatattcg cttactgagg cgactgaaca tatagttcgt ggcatatctt tctaagtaat
     2521 taggggtatg gattgacttt gtagcataag cgtgctatat tcaattttag caatgaagtc
     2581 tgcagtacct actaccgaag ttaaagtcca agagaagaaa ccatacagcc caaagatggc
     2641 ggtagtaaac atcgtcatgt tcagcagcct ctttcttgtt tggctcgttc tacaattgca
     2701 aaaatagcag caatgacgtc ttgtatagcg aaaattacca cgaccttata aagggcagtg
     2761 gtaattttta tatctgttag cctaactcgt tatccacaaa tctgtggata aatgtaacca
     2821 caatacgaaa agttgtcaaa aaatggtatt gcagttgtaa tcagaccagt ttatgatgag
     2881 ttcagtggaa taagcgtaag cgattgcgca caacacaaac accacaacaa taaaaataca
     2941 aaaaatcacc gcaaggtgaa gggtacggac taaaccatga cacaacctat cagccaacta
     3001 ccacacaagc acgtagccgg ttgtattatc gcgccagcac gtgggtagtt aacgccttat
     3061 tatttctgat acctaaccgt ttttctgcac tttaacaaat tagcgacatc ctcgagatca
     3121 acaatctggt ggtgaaccag tggtctatcg acccacatta aaaatagtcg aatgtacctt
     3181 cctctaacac aacacctccc aaaaaactcc acctcacaca taagtctctc ttagataagc
     3241 aaatcggact atgggtttct tagaaatcac cgtcggcgct tatctttact tatacacaaa
     3301 aaacaaacac accatgcgaa aacaaacata ggtttgctac cagtttggtg atctcgagtt
     3361 gacaaaaaga ggaagggcac gcccttccaa tgcatcatcg aagtaaattc gctggcgcat
     3421 ttccatccct tcaagctcgc ttcgctcgtg cggatacagc ctgggaatgg tatacttttg
     3481 ttatatgagt ataaaagaac atccaccact tcatgttcct gtgttactcg agtcggtcat
     3541 tgcattactg caacctcaat taggcgagaa ttatctcgac ctgacggcgg ggtatggtgg
     3601 tcatgcgaga gtcgtactgg agaagacaga taattatatt gaatcagcct tggttgatcg
     3661 cgatgatttt gcgattgcac acctcggtga gttttctgac aaagggactc gtttactgca
     3721 taccgactat ctcagtgctg caaaacagct agtagaagaa gggaagcaat tcgatataat
     3781 attaattgat ttgggggtgt cgtcaccaca gcttgatatg caagcaagag ggttctcgtt
     3841 taatcgcgat ggacctctcg atatgaggat ggataatcga caaagcttaa cagctgagac
     3901 gattgttaac actgcaaaac gcaatgagtt aaccagaatc atacgagaat atggcgagga
     3961 gtcaccaggc actgcaaaac gctatgccga agcgattatc gccgctcggc ctatacaaac
     4021 gactgggcag ctcgcggacg ttatcaagca atctcatgtc ggcaagtggc agaaaaccca
     4081 cccagcgaca cgtacctttc aggccatacg gatccaggtc aacgatgagc tccaccaggt
     4141 tcgtgaagtg atgccgctcc tccctcgatt actgaaaaaa ggtggtcgag ttggcgtcat
     4201 tagctttcac agcctggagg atcgcatcgt aaagcgctat tttgccgaac agacaagaag
     4261 tggctacgaa gcagagctcg aaatcgtgac caagcgtcca attgatggat ccattcagga
     4321 tgttcacaac ccgcgatcac gcagtgcaaa attgcgcgtc gcagtgaaaa aataaaacaa
     4381 aaaaggaggg gtaactgcat gccagtacat atcccagtac aataccaaga cgaatctgca
     4441 aacgtagacg tacacgttaa gtagtattcg caaccgctct tacggagagc aaaaagctct
     4501 ccgtaaggca ccaagtaaac aaacacaaaa agaaaccacc acaatgtcat ataaccgaac
     4561 cgtacaattt aatagcaggc gccaaactaa ctggcagcgc aatcaaaata cagttgcttt
     4621 tgcctctgct gtaaaactcg gcccaatcag ccacaccgta ctcgttgctc ttatgattac
     4681 agtgctaggt ttgatatact taacacaggc tactcgagtg tctgggtatg attatgcagc
     4741 caatgcaatc tcagacaaga tttcttcatt gaccgaacag aaggataacc tggaagttga
     4801 gaatgctcgt cttacggcac tgcaaactgt caaaaactct aacgttgcaa aggcaatgac
     4861 gaccccgaca gatgttcaga gcgtgagtga gtagtaccta acccctaaaa ctcatggaac
     4921 tgcatttttc ccaaggaagt cgcgcgaaaa agattgctgt agtaaccctt gctgcaatgg
     4981 cgatttttat tgtgcgcctt ttttatattc aggtggtgca gcatgagaag tatgttgcac
     5041 aggcggatca ggagcaagtg aagcaattta cactccacgc caaacggggt gaaatttata
     5101 caatggacgg ctccacgccg gcaaagctgg tgatgaatca gacagtgtac actgtgtggg
     5161 ctgacccaac agaggtaacc gacaagaacg ctgtaatatc ggcacttaac agtattgccg
     5221 ggggaaatgt acgtgataat tttgcgcaat atctcgatat gagccattcg cgctatcagg
     5281 tgcttgcaac aaaagtgacg atgcagcaag cagaaatgct gaagaagaaa cgcttagctg
     5341 gtattggatt cgatggggtg agccaacggg tttatcccga gggccaacta gcgagccagg
     5401 tgcttggatt tgttgatgca aacggtgagg gtaaatatgg ctttgagcaa gcaaatgatg
     5461 cacaactaaa gggcaaggac gggctgctta agacggtgac agacattcgc gatgtgcctc
     5521 ttacagtatc tgataaaaac attcgtcaac ctgcagtgaa cggccagaat atggtgctga
     5581 cgattgatcg taatattcag gctgagacgg aacaagcgct tgcggacggt gtggcaaaga
     5641 gtggggctga ccacgctagt gcaattgtta tggatccaca aaccggcaag gtgcttgcaa
     5701 tggcgaatgt gcctacctat gatccgtccc agctcaataa ggtaactgac gttgcacagc
     5761 ttaacaatga tattgtttca gcaccatacg aacctggatc tgacattaag acatttactg
     5821 tggcgacggg tgtcgacaaa ggagttatca ctccaggttc aacgtataat aatactggac
     5881 agattaaggt cgatgatatc acgattaaca atgctacaaa aacggcagcg gtaacgggca
     5941 atattacaat gcaaacggca ttgaactggt cacttaatac aggtatggtg acgattgccg
     6001 agcgacttgg tgatggcaat tatattacgc gccaggcacg cgatac
//
LOCUS       DATIIX010000055         5890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_320039, whole genome shotgun sequence.
ACCESSION   DATIIX010000055 DATIIX010000000
VERSION     DATIIX010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5890
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320039"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..922
                     /locus_tag="VLF64_01620"
     CDS             230..922
                     /locus_tag="VLF64_01620"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HSW90675.1"
                     /translation="MDSDTTRLNKLLALQLGVSRREADILIDTGSVTVNGKVAELGGR
                     ASESDSITVAGKPIATKKTPYVYLALNKPVGYVCSRRPQGDSPTIYSILPHEYHALKP
                     VGRLDRNSSGLILLTNDGDFAFKMTHPKFAKTKVYHVRIDHDLEPLHQQMISDFGVDL
                     EDGHSQLTLARLSDNRRDQWEVTMSEGRNRQIRRTFAALGYTVKELHRVQFGNYSLGM
                     LQPGDFQFTTVQ"
     gene            complement(1047..1697)
                     /locus_tag="VLF64_01625"
     CDS             complement(1047..1697)
                     /locus_tag="VLF64_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90676.1"
                     /translation="MFRGLFRQHTRTIINERGNSVTVPATCTEVNSLISSHVLLQPED
                     DGKGGLRMVREVVSCASVNEEFTRTVFANEQLTSQVERLTAEVETLTRERDNCNRLMA
                     GALQVIFLMGPALQGLLADDLGSPELTDDRSAVRLTGAAYTIVLDVFGEQIIVRPAIA
                     DQAVPEKIFEVPNGRLGENARTLLLGYITRIKAVAAGATDPVRGEIDLLAPAGAVK"
     gene            2499..2912
                     /locus_tag="VLF64_01630"
     CDS             2499..2912
                     /locus_tag="VLF64_01630"
                     /inference="COORDINATES: protein motif:HMM:NF016564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsgA family sporulation/cell division regulator"
                     /protein_id="HSW90677.1"
                     /translation="MDYPTRIVGVLQDEHEQRLLVPTDFGHSLRDPYAVHLNFNPGSD
                     HKEWIVSIELFTEAFDRPDRTHGYGDVTITYYPTDNQLWIGLQPPEGVATIAFPATAV
                     AEFINSTRNSATDNVRETALDIFFEKVEKCANQSW"
     gene            3069..4532
                     /gene="der"
                     /locus_tag="VLF64_01635"
     CDS             3069..4532
                     /gene="der"
                     /locus_tag="VLF64_01635"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HSW90678.1"
                     /translation="MASKLPTVAIIGRANAGKSSLFNRMVRSQQAIVAREAGTTRDNV
                     LGKVSYSRRNTGEDGQTGEFWLVDTAGLKDPNDEFEAGIQDQIQEAADAADVILVVVD
                     STEYPGDEDRTVAKKALKSKKPVILVTNKADLKDAIHNDEFRRLGIKDIIRTSAEHNS
                     GVSELLDKIAEIIPEAHQAEADDILRVALIGRPNVGKSHLFNTLAGKQQALVANVAGT
                     TRDLNRVQIGYHQQTIELVDTAGIRRQGKQEVGIEKFSVLRTLNAIEEADICFLLMDV
                     NELNVALDQKLAGIIAEAGKGLVLVVSKWDSVMDKDAFTRDALAPKIAHNFDFVPWAP
                     LIFTSSVTGQNVTKLFDLAIDIRTRREQETKTRVLNDVLQNAVAKHPPAGLKNTHPKL
                     RYMVQTDTLPPWFVVYGSNLKFIHWSYKRYIERLVREAYNYSGTPIKFSYRDEKQIKS
                     NKERVAKGLEPVTKAYKQRKEVEKHEQRQLERESGEE"
     gene            4536..5102
                     /gene="pth"
                     /locus_tag="VLF64_01640"
     CDS             4536..5102
                     /gene="pth"
                     /locus_tag="VLF64_01640"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HSW90679.1"
                     /translation="MKVIIGLGNPEEKYRGTRHNVGFWVLEALADARKVSFKLETKFN
                     ARIATVAIAGEKILLVQPQTYYNEVGISARGLLDFYKLTTKDFLIIHDDLALPLGTIR
                     TRLGGSDGGNNGLKSLAAHIGANTARIRVGVWVEHHHGADKVGIVLGRFTHDEQKELA
                     QELPIIQRITQDFISDTFEATTHKPQKS"
     gene            complement(5178..5354)
                     /locus_tag="VLF64_01645"
     CDS             complement(5178..5354)
                     /locus_tag="VLF64_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90680.1"
                     /translation="MFLLIMIGLTETGRVLWAQLPMVLALLWCGLAGLVETASDWQRE
                     ILRWPIDSARDHDG"
BASE COUNT         1566 a         1674 c         1408 g         1242 t
ORIGIN      
        1 ccgcagcaat gccaacacct ccaactccaa ctccaatgcc tgtggcgcca gtcgcaccaa
       61 atcccacgcc aatgcccact cctccaagtc ctcctatcac cccggttgcg cccccttcaa
      121 caggcctcaa tttcgaagaa actccccgtc caaacatgca acctccaaca gctcctccat
      181 tgccacctat tccgccagcc cctaatactg ggctataata gccactacta tggatagcga
      241 tacaacgcga ctcaacaaat tactcgcgct acagcttggc gtttctagac gtgaagcaga
      301 tattcttatt gacaccggct ctgtcacggt aaatggcaaa gttgccgagc taggtggacg
      361 agcgagcgaa tccgatagca ttaccgttgc cggtaaacca attgccacca agaaaacacc
      421 ctatgtttac cttgcgctca ataaacctgt tggctacgta tgctcacgcc ggccacaagg
      481 ggatagtcca accatctaca gtattcttcc gcatgaatac cacgcactca aaccagttgg
      541 gcgactcgac cgcaatagtt ctggcttaat tctccttaca aacgacggtg actttgcatt
      601 taaaatgaca caccctaaat tcgctaagac aaaagtctat catgtccgca tcgatcacga
      661 tcttgagccg ctccaccagc aaatgattag cgactttggt gtcgaccttg aggatggcca
      721 cagtcaacta acactcgctc gcctcagcga taatcgccga gatcaatggg aagtaaccat
      781 gagcgaagga cgcaaccgtc agattcgacg tacttttgcc gccctcggtt acaccgtcaa
      841 agaacttcac cgcgtacaat ttggtaatta ctctctcggt atgctgcaac ccggtgactt
      901 tcaattcaca acagtgcagt aagataatac aaaaaaccac caaatggtgg cttaaataat
      961 ttcatggtgg tgctggcagg ccagccagct cgggattgtc gctcccgagc cagactggcc
     1021 atgccgtcac ctgagggtgt aagagatcac ttgactgcgc cggccggagc cagcaggtcg
     1081 atctccccgc ggacggggtc cgtggcgccg gccgccaccg ccttgatccg cgtgatgtag
     1141 ccgagcagca gcgtacgtgc gttctctccg aggcgcccgt tgggaacctc gaagatcttc
     1201 tcgggcaccg cctggtcggc gatggccggg cgcacgatga tctgctcgcc gaagacgtcg
     1261 aggacgatgg tgtacgccgc accggtgagc ctcaccgccg agcggtcgtc ggtcaactcc
     1321 gggcttccca ggtcgtcggc gagcaggccc tggagggccg gccccatgag gaagatgacc
     1381 tggagcgcgc cggccatcag ccggttgcag ttgtcgcgct cccgcgtcag cgtctcgacc
     1441 tcggcggtga gccgctccac ctggctggtg agctgctcgt tggcgaagac cgtacgggtg
     1501 aattcctcgt tgaccgaggc gcaggacacg acctcgcgga ccatccgcaa gccgcccttg
     1561 ccgtcgtcct ccggctggag gaggacgtgg ctgctgatga ggctgttgac ctcggtgcag
     1621 gtagccggga cggtcacgct gttgccacgc tcgttgatga tcgtgcgagt gtgctggcgg
     1681 aacagaccac ggaacatggt tgacctccaa ggtcacggcc catacactcc tacccggttt
     1741 gggcggagca gggattacaa agttcatgtg ttataaactg tgtaatccct gctccgttga
     1801 tctctcgtat ataaacgttc atgccctcag gcaatggggt ctattatact atatataatt
     1861 atattgtcaa tattcttttc agtcattccg gagtaactcc cattaaccac ataaacctgc
     1921 aacctatatc attgactatg ataacaaaat atgttaagat ataatttagg ctcggacaga
     1981 ccgagatatc gagcagggat gagacagata gtgagcaagc gtgccacgcg gcgtgaagca
     2041 ctcctgtaca tgatcaaaga ccgccacaac ccgaccgagc atgtcgccac cgattggacg
     2101 gcgatatgga gacggggaga gaggtggctt tgcacttact gcggtcatcg gatccccacc
     2161 aatggggttc cgatcatcga gacacggaag caaccgtggc gaatcccgag cgagatgatc
     2221 acgcgattcc gtgcaatgcg gcgccatcaa gaggcatagc ctctcgtggt caccgccctt
     2281 acgggcgtca gcaacagctg gcgccctatt tcttttagca gcgcctcaat ctcaatcacc
     2341 aataaccgat aaatgagtgc agtagataca aaacatctat aaagtccctt aagacacctt
     2401 cgttaaggag ggtggacaat ctccagatat tgacatatat tatataatag tttatagtga
     2461 cactgcctta tcagctacaa gtggcaagga gcatcggcat ggactatccc acacgaatcg
     2521 ttggcgtcct acaggacgaa cacgaacaac ggttgctcgt ccccaccgat ttcggacaca
     2581 gtctgcgcga cccttacgca gttcacctca acttcaaccc aggcagcgat cataaagaat
     2641 ggatcgtgtc tatcgaactc ttcactgaag cattcgatag gccagataga acccatgggt
     2701 atggtgacgt gaccatcact tattacccta cggacaacca gctctggatc ggactacagc
     2761 ctcccgaggg agtcgcgaca atcgccttcc cagcaacagc ggtcgcagag ttcatcaact
     2821 ccacacgaaa tagcgccacc gacaacgtca gggagacggc cctcgacata tttttcgaaa
     2881 aagtcgagaa gtgtgcgaac cagagttggt gacccgcgcc aagctgaaag gctctccggc
     2941 tcccctcaat cagaggggga gccggagata gcccatagct aaaaaactcc ctcaacgcaa
     3001 gcctacaaca ccattaatac cgcttgcgct cgcaagcttt ttttatttct gttataatta
     3061 cctctaatat ggcttcaaaa ttaccaacag ttgcaattat cggtcgggct aatgcgggca
     3121 aaagctcgct ttttaatcgt atggtccgct cgcagcaagc aattgttgcc cgcgaagccg
     3181 gcaccactcg tgacaatgtc ctcggtaaag taagctacag ccgccgtaat actggcgaag
     3241 atggccaaac cggcgaattc tggcttgttg ataccgctgg cttgaaggac cctaacgacg
     3301 aatttgaagc gggtattcaa gaccaaatcc aggaagcggc agatgccgcc gatgtaattt
     3361 tggttgttgt tgatagcact gaataccctg gcgacgaaga tcgtactgtt gcaaaaaaag
     3421 cacttaaaag caagaagccg gtcatcctcg ttaccaacaa agctgacctt aaagatgcaa
     3481 ttcacaacga cgagttccgc cgccttggta tcaaagatat cattcgcacc agcgccgagc
     3541 ataattcagg cgttagtgaa ctgctcgata agatcgccga aattatccca gaagcccacc
     3601 aggcagaagc tgacgatatc ctccgcgttg cacttatcgg tcgcccgaac gtgggcaaga
     3661 gccatctttt taacacgctc gctggcaagc aacaagcact tgttgcaaac gtcgccggta
     3721 ccacccgcga cctcaaccga gtgcaaattg gctaccacca gcaaaccatc gaacttgtcg
     3781 acaccgctgg tatccgccgc cagggaaaac aagaggttgg tatcgagaaa ttctcagtcc
     3841 ttcgtacact caacgcgatc gaagaggccg atatctgttt ccttttgatg gacgtaaacg
     3901 aactcaatgt agcccttgat caaaagctcg ctggcattat tgccgaggca ggtaaaggac
     3961 ttgtgctcgt ggtcagcaag tgggacagcg ttatggataa agacgcgttt actcgcgatg
     4021 cgctagcacc aaagattgct cataacttcg actttgtacc gtgggcgccg ctgattttta
     4081 ccagtagcgt cacgggacag aacgttacaa agctttttga cctcgccata gacatccgca
     4141 cccgccgcga acaagagaca aaaactcgcg tgctcaatga tgtcctacaa aacgccgtag
     4201 cgaagcaccc gccagcaggc ctcaaaaaca cccatccgaa gctccgttac atggtgcaaa
     4261 ccgacacgtt accaccttgg tttgtcgtct atggctcaaa cctcaaattc atacactgga
     4321 gctataagcg ctacattgag cgactcgttc gtgaagccta caactattct ggtacgccaa
     4381 ttaaatttag ttatcgcgac gaaaagcaga tcaagagcaa caaagaacgt gttgctaagg
     4441 gcttagagcc agtcacaaaa gcctacaaac aacgcaaaga agtcgaaaaa cacgaacagc
     4501 gccagctcga gcgcgagtcc ggagaagagt agctcatgaa ggtcattatt ggcctaggta
     4561 accccgagga aaaataccga gggacccgtc acaacgttgg attctgggta cttgaagcac
     4621 tcgctgatgc ccgtaaagtt tcgtttaagc tagaaactaa gtttaatgca cgtatcgcca
     4681 cggtggccat tgcaggtgaa aagattttac ttgtgcagcc gcaaacgtac tacaacgagg
     4741 taggcatcag cgcccgcgga ctgctcgatt tctataagct caccacaaaa gatttcctta
     4801 ttattcacga cgaccttgca ctaccactcg gcaccatccg tacacgtctt ggcggcagcg
     4861 acggcggcaa taatggcctg aaatcacttg cagctcacat tggcgccaat actgcacgta
     4921 ttagagtagg agtatgggtt gagcatcacc acggcgccga taaagttggc atcgtactcg
     4981 gtagatttac tcatgatgag caaaaagagc tcgcccagga gctcccaatt atccagcgta
     5041 tcacgcaaga ttttatttca gatacatttg aagcaacgac gcacaagccc caaaaaagtt
     5101 gaatagcccc cgccgatgat ggcgagggct accccttgag tacggaagaa ttcacgtatg
     5161 ggtggcgcag cgccatatta cccgtcgtgg tctctggcac tatcgatagg ccagcgcaaa
     5221 atctcgcgct gccagtcact cgcagtctcg actagaccag ccagaccgca ccagagcaaa
     5281 gcgagtacca tcggcaactg ggcccaaagc acgcggccag tctccgttag accgatcatg
     5341 atcaaaagga acatgccggc caccgcacac atagcaccta ccacccaacg ctcgcgttgg
     5401 gatttactct ttggcaatcc tgcgcgcgac tctttcgatg caaagtgctt acgcacccac
     5461 atctttaccc gcccctcccg ataaggggca tccgagcctt cggcactcat aggtaagcac
     5521 catccttcac taaggggctg tacgcccagg ttaccaagca atacgtaata aaactatata
     5581 attaaactgt atataaagca acctttcaac acacaacacg caccagagag ataggaagct
     5641 gttaagtgac ctcaaaacag gaacgagccc tccttatttt ttccaaccaa aaagcgccag
     5701 gtaagagtgg gcatcacctg gcgccatttg gacccttcat cccaccctga tcgggcatag
     5761 cgtgacccac ctcacaacat atggaggtcc gccggataaa ggggttagta tactcactaa
     5821 agcttgtcta acggtgatgt agtggagggt agaatactgc atcacaccat agtgcgcaac
     5881 ctaacataag
//
LOCUS       DATIIX010000056         4785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_236454, whole genome shotgun sequence.
ACCESSION   DATIIX010000056 DATIIX010000000
VERSION     DATIIX010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236454"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VLF64_01650"
     CDS             complement(<1..1161)
                     /locus_tag="VLF64_01650"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HSW90681.1"
                     /translation="MKVSLNAVKQYIDFELPPVDELVKRINEQLGGIEEIIDLGARYK
                     GAVIVRVTECDKHENSDHLHKCLVDDGGVTPNVERNERGLVQVLTGAPNVHKDMLAVW
                     LPPKTIVPETFDTTEPFMLESRNMRGAMSHGMLASARELAIGDAHEGIIDITNERPAE
                     GGKPVQPGSLFADAFGLNDTIIDIENKMFTHRPDGFGQLGVAREIAGILGYKFTSPEW
                     YINQPSFASANGLDLEVFNDAPELVPRFMTMAIDGVEVKESPLWLQCALTTMGSRPIN
                     NIVDATNYIMLTTAQPTHAYDYDKLRGHRLGARMAHSGESITLLNDKTYQLHDTDIVI
                     ADGEGPVGLAGIMGGGESEVDKSTTRIVLEVATFDMYAVRKSSMRHGVFTDAL"
     gene            complement(1161..2192)
                     /locus_tag="VLF64_01655"
     CDS             complement(1161..2192)
                     /locus_tag="VLF64_01655"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF013568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HSW90682.1"
                     /translation="MDIETLKNTLLQRVGESKTPNEVLKASEFRELYAKIPTLAPEER
                     GPFGRQVNELKQVIEAAVQAREAILEESNIRPIDVTAPFDINAKMPTLLPSEDGTTHP
                     LMQEIQLVSDIFNRMGFVTEESREIDDQYHMFETLNFPKGHPARDDYDTFMTVETDKD
                     GDPFIAPAHTSTMQNRTLVKYRDNLANGGAIAAIVPDRVFRNEDLDARHEHTFYQVEG
                     IYVAKSVNVGNLVATLQAFLEEYYGRTLKTRINPFYFPFTEPSFEFSLECPFCEVGCN
                     ICSQSKWIELLGCGMIHPNVLKAADIDSNEYTGFAFGCGIDRLVMMKYGIEDIRHLES
                     GKLNFLRQF"
     gene            complement(2203..2463)
                     /locus_tag="VLF64_01660"
     CDS             complement(2203..2463)
                     /locus_tag="VLF64_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90683.1"
                     /translation="MGRYIEQTEGQSELQQRIAAELKAKAAARAKQEGDSNEGYHKAP
                     DGVEDAAYMYGTKRTTTLAPVWAIIFFLAIGVFIYFIYKMSH"
     gene            2571..3503
                     /locus_tag="VLF64_01665"
     CDS             2571..3503
                     /locus_tag="VLF64_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HSW90684.1"
                     /translation="MATKKAASTKKATAKKPAAANTTVRTVRAEAKPEAAPVVRTAVT
                     SGKSGVKLPSNIINIVLAELVGTFVLTLVALATFTDSGALYVGLALSVLVFAVGAVSG
                     THINPAVTFGLWATRKLKSILVPFYWGAQLLGAMLALIVTNWVSDSSTNLSFNHFWNF
                     NWGIFACELVGAAVFLFGLTAVLTRRELSAGVRAIGTGLTLTIGLVAGGSLLSTLKAQ
                     DVAAYQKSSQSSQTDKSVKTPSVPYSAYVKGITANPAIALAVTNHSDSELGVSTTNST
                     TYSYFTSSVILGTLIGAALGGNLYLLIVGRNKND"
     gene            complement(3592..4656)
                     /locus_tag="VLF64_01670"
     CDS             complement(3592..4656)
                     /locus_tag="VLF64_01670"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HSW90685.1"
                     /translation="MEVIVSILLVLVIAVFGVTLYGLTRKLNEMKQSSAVELLKSDVT
                     ELSRSIAALNQSMGDRLEKSSLGVQQSVQRQLSESAKLVADVTQRLAKLDETNARVVG
                     VADELKTLQNVLSNPKQRGVFGEFYLSSVLENVLSPGQFQTQYKFSDGEIVDAVIFLD
                     KGQILPVDSKFSLENYNRMIEASGKEREQYMQQVKADLKGRIDETSKYIRPSEKTMDF
                     AFMFIPSESLYYDLLINNVGSGGSSRDLIEYAFREKHVIITSPTSFMAYLQTVLQGLR
                     SLQIEEQAKDIQVRVGKLGQHIAKFDEYMIKLGNSLGTTVNHYNSAHKELAKVDKDVV
                     KIASADPSVEAILLDKPSQE"
BASE COUNT         1207 a         1192 c         1047 g         1339 t
ORIGIN      
        1 aagagcatct gtaaatacgc cgtggcgcat actacttttg cggactgcat acatatcaaa
       61 ggttgcaact tcgagcacaa tgcgcgtcgt tgatttatcg acttcactct cgccgccacc
      121 cataatgcca gcaagcccga ctgggccttc gccgtcggcg atcacaatat cggtatcatg
      181 tagctggtag gtcttgtcat tgagcagtgt aattgattcg ccactatggg ccatacgtgc
      241 acccagtcta tggccgcgaa gtttatcgta atcatatgca tgcgttggct gtgcggtcgt
      301 gagcattatg tagtttgtcg cgtcaacgat gttgttgata ggacgagacc ccatcgtagt
      361 aagcgcacac tgtaaccaga gtggactttc cttcacttca acaccgtcaa tggccattgt
      421 catgaagcgc ggaacaagtt ctggcgcatc attgaacact tcaagatcaa gtccatttgc
      481 gctagcaaaa cttggctggt ttatatacca ctcagggctc gtgaatttgt accccaaaat
      541 accggcgatt tcacgcgcta cgccaagctg gccaaatcca tcagggcgat gcgtgaacat
      601 tttgttttcg atatcgataa ttgtgtcgtt tagaccaaat gcatcagcga acagcgagcc
      661 aggctggaca ggcttaccac cttctgcagg tcgctcattg gtaatatcaa tgatgccttc
      721 gtgtgcatca ccaatcgcta gttcacgcgc gcttgcaagc ataccgtggc tcatcgcacc
      781 gcgcatatta cgactttcga gcataaatgg ctcggtagta tcaaaagttt caggtacgat
      841 tgtttttggc ggaagccata cggcaagcat atctttatgt acgttagggg cgcctgtgag
      901 gacctgaacc agccctcgtt cattgcgctc aacattgggc gttacgccgc catcgtctac
      961 caagcatttg tgtaagtgat cggagttttc gtgcttatca cattcagtaa cacgtacgat
     1021 aaccgcacct ttgtagcgtg cgcccaaatc gatgatttct tcaatgccgc cgagctgctc
     1081 gttgatacgc tttacaagtt catcaacggg aggaagttcg aaatcgatat attgttttac
     1141 tgcgtttaga ctaactttca ttagaactgc ctcaagaaat taagtttacc agattcaagg
     1201 tggcggatgt cttctatacc atatttcatc atgacgagac ggtcgatgcc acacccaaag
     1261 gcgaagcctg tgtattcatt tgagtcgatg tcggcagcct tcagaacgtt ggggtggatc
     1321 atgccgcagc caagcaattc gatccatttg ctctgagaac aaatattaca accgacttcg
     1381 cagaacggac attcaagcga aaattcaaac gaaggctcgg taaacggaaa gtaaaatgga
     1441 ttaatacgag tctttagcgt gcgaccataa tattcttcaa gaaacgcttg caatgtcgcg
     1501 actaaattgc cgacattcac actcttcgcg acatagatac cttccacttg gtagaaagtg
     1561 tgctcatggc gagcatcgag gtcttcgtta cggaatacgc ggtccggtac gattgcggca
     1621 attgccccac catttgcgag attatcgcgg tattttacaa gagtgcggtt ttgcatagta
     1681 ctcgtatgcg ctggagcaat gaacggatcg ccatccttgt ctgtttcaac tgtcatgaag
     1741 gtatcataat cgtcacgcgc aggatggccc tttgggaaat taagtgtctc aaacatgtga
     1801 tactgatcgt cgatttcgcg agattcttct gtgacaaatc ccatgcgatt aaaaatatcg
     1861 gatacgagct gaatttcctg catcagtgga tgcgtagtgc cgtcttcgct cggcaacaat
     1921 gttggcattt ttgcattaat atcaaacggt gcggttacgt cgatcggacg aatgttggat
     1981 tcttcaagta tagcctcacg agcctggact gcggcttcta tcacctgttt gagttcgttt
     2041 acttggcgac caaacggacc gcgttcttca ggtgcaagtg tggggatctt cgcatagagc
     2101 tcacgaaatt ctgacgcttt caatacttcg ttaggtgttt tcgattcgcc cacgcgttgt
     2161 agcaatgtgt tctttagtgt ttcgatgtcc ataggtcccg ctctaatgac tcattttgta
     2221 gataaagtag ataaatacgc caatcgctaa aaagaaaatt atagcccata ctggtgcaag
     2281 cgttgttgtg cgctttgtac catacatgta tgcagcatcc tcaacaccgt ctggtgcctt
     2341 gtgataacct tcatttgaat caccttcttg tttggctcga gccgccgctt ttgccttgag
     2401 ctctgccgca attcgttgct gcaattcact ctggccttcg gtttgctcta tatatcgtcc
     2461 catgtctatt atcatacact acttcactct gttaatcgat agacggttgc acctgaactg
     2521 tttgtgctat acactggagc tatatcttag ttaaaggagg gatataatca atggctacca
     2581 agaaagccgc ttctactaaa aaagcgaccg ccaaaaagcc ggccgcagcc aacacaactg
     2641 ttcgcactgt tcgtgctgaa gcaaaaccag aggcagcgcc agttgtgcgc accgctgtaa
     2701 cttcaggcaa gagtggcgtt aagttgccat caaacatcat taacattgtg cttgccgaac
     2761 ttgttggtac gttcgtcttg acactcgttg cacttgcaac attcacagac tctggcgccc
     2821 tctacgtagg tcttgcgctt tctgtgcttg tatttgctgt tggtgcagta tctggcacac
     2881 acatcaaccc agcagttacc tttggtctct gggctacgcg taaacttaag tcaattctcg
     2941 taccatttta ctggggcgcc cagcttcttg gtgcgatgct tgcacttatt gttaccaact
     3001 gggtatcaga cagcagcaca aaccttagct tcaaccactt ctggaacttc aactggggca
     3061 tcttcgcatg tgagctcgtt ggtgcagccg tattcctatt cggtcttact gcagtactaa
     3121 ctcgccgtga gctttcagcc ggcgtacgtg cgatcggcac gggtcttacc ctcacgatcg
     3181 gacttgttgc tggcggttcg ctgctttcaa cacttaaggc tcaagacgtt gctgcttacc
     3241 aaaagagctc tcagagttca caaacagaca agagtgtaaa gactccaagt gttccgtact
     3301 cagcatacgt aaagggtatt acagcaaatc cagcaattgc gcttgccgta actaatcact
     3361 cagatagtga gcttggtgta agtacaacca attcaacaac atacagttac ttcacatcat
     3421 ctgtaattct aggtacactt atcggcgcag cacttggcgg taatctttac ctccttatag
     3481 ttggccgcaa taagaacgac tagtcctagg ctattcttca ataaaaatac tacccgcacc
     3541 aaacgggtag tatttttatt gaagatcctg agtgaaaagg agtgggcgta tttattcctg
     3601 cgatggcttg tcaagcagaa tcgcttcaac gcttggatca gcactcgcaa tctttacgac
     3661 atctttatct accttagcaa gttctttatg cgcagagttg tagtggttta ccgtggtacc
     3721 aagtgaattg ccaagcttta tcatatactc atcaaacttc gcgatgtgct ggccgagttt
     3781 gcctacacgc acttgaatat cctttgcctg ttcctcgatc tgaagactac gcagaccttg
     3841 tagcactgtt tgcaagtacg ccataaagct ggtcgggctt gtgataatca catgcttttc
     3901 ccggaacgca tactctatca aatcacgtga gctaccgcca ctccccacgt tattaatcag
     3961 caaatcatag tagagtgatt cgcttgggat gaacataaac gcgaaatcca ttgtcttttc
     4021 actcgggcga atatatttag atgtttcatc gatacgccct ttcaaatctg ctttcacttg
     4081 ctgcatatac tgttcgcgct ctttacccga tgcctcgatc atgcggttgt agttttcaag
     4141 actaaattta ctgtctactg gcaagatctg tcctttatcg agaaagataa cagcgtcgac
     4201 gatttcacca tcgctaaatt tgtactgcgt ttggaattgt cctggcgaca agacgttttc
     4261 aagaacactc gaaagataga attcaccaaa cacaccacgc tgcttcggat tggaaagcac
     4321 attctgtagc gtttttaatt catctgccac gccaacaacc cgtgcattgg tctcatccag
     4381 cttagcgagg cgctgggtta catcggccac aagtttggcg ctttcactca gctgacgctg
     4441 caccgactgc tgtaccccta agctgctttt ctcgagtcta tctcccatgg attgattaag
     4501 cgccgcaata cttcttgaaa gctcggtcac atcgctcttt agcagctcta ccgcgcttga
     4561 ttgcttcatt tcgttaagct tacgcgtgag cccataaagt gtgacaccaa aaacggcaat
     4621 cactaacacg agcaagatac ttacaataac ttccatatag ccccaccttc tgtcacacta
     4681 ggcaacgagt atccaatgaa ttagtacaac agcaacaacc gttacgacga tcgccaaagt
     4741 aaaattacga attcgcgcaa gccatgcata tagtccatat gacaa
//
LOCUS       DATIIX010000057         3102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_431164, whole genome shotgun sequence.
ACCESSION   DATIIX010000057 DATIIX010000000
VERSION     DATIIX010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431164"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1587
                     /locus_tag="VLF64_01675"
     CDS             <1..1587
                     /locus_tag="VLF64_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HSW90686.1"
                     /translation="HQYKYYITTKDGHTLEKNDPRARAITSSDNGFSVIVDNEFDWGD
                     KTFEPLPKNQQIIYELHVGTFNRPDASTPGTFDTAIEKLDYLAQLGITTIELMPITSM
                     AYSNGWGYAPNYIFSVESMLGGRYGLMSFVKACHERGIGVILDVVYNHFYGESDLWQF
                     DGWCENNRGGIYFYNDTRGDTPWGGRPDYGRPEVRQFILDNITMWLNEYHLDGLRIDS
                     TIYMRNTEGRDNDPEHDIPDAWFLLQDIVEVAHKINPKSLIIAEDCANSSYITCNRSQ
                     TGCGFDAQWELGFPHVLRDGLGLNPNSTPTLSGVRYELERNYNGDAFEKVIFSDSHDT
                     AANGSVRLNDAITPENPTSLAAKQKTLLAACMMFTAPGIPMMLQGAEFLQDGAFNDWQ
                     ALEWNHLTAHAGFTLAHQHLTALRKNIYSNSVGLQGQYTAVFHQDDNNHVIGFARATN
                     SDSNDSTIVIANFSNLNLRTYQLHLPGLGTWNVRFNSTWKGYSPDFPEVALHEIITDN
                     TGMATIQLAPYSVYIASKQS"
     gene            complement(1671..2147)
                     /locus_tag="VLF64_01680"
     CDS             complement(1671..2147)
                     /locus_tag="VLF64_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90687.1"
                     /translation="MTKSSEMERKECEQMMLAPTPMELFKLQLEAAKKSWQKGEALLP
                     EIKALEAVFLAELQDARQRATTYAALSTQQYRLATGHLRSGRSRKGHTKLARSRSFAG
                     QARQAWRERRVWGRRLHELCQIRRAVKARKKHYFRLLAAARNLLPTVSDRELATII"
     gene            complement(2212..3048)
                     /locus_tag="VLF64_01685"
     CDS             complement(2212..3048)
                     /locus_tag="VLF64_01685"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HSW90688.1"
                     /translation="MIEQLRVVTWNAEGMFVAGSNTRRATPQMAIATIKRLNADVVFI
                     PEFGNTDAIEEATLMALRALGYEIVFFSYGHSWLGSYGAALLSRLPLKSYLIHHFTNT
                     QRPFIEATVDDNGTLLRIVGIHLDDRSEALRLQQVPEVAKVVNQNDSLPVIVLGDFNA
                     MHASSKFARAVRARPLYYAARLFPHTQLRGMGSRVSEMGRGTAITELLKRTDLHDLDG
                     LHRRTISAKQSGLEWMPAWRLAKIDWIFGSKGIKAVSYKISSDVGSDHRPVIAELDIK
                     RP"
BASE COUNT          778 a          805 c          740 g          779 t
ORIGIN      
        1 catcagtaca agtactacat tacaaccaaa gatggccaca cgttagagaa aaacgacccg
       61 cgcgcacgag ctattacatc aagcgataat ggcttttcgg taattgtcga caacgaattt
      121 gattggggcg ataaaacttt cgaaccactt cccaaaaatc aacagattat ctatgagctc
      181 catgtcggta catttaatcg tccggacgcc tcgacgccag gtacattcga tacggcgatt
      241 gaaaaactcg attaccttgc tcagcttgga attacgacga tcgaattaat gccaattact
      301 agcatggcct acagcaatgg ctggggttat gcgccaaatt atatttttag tgtagagagt
      361 atgcttgggg gccgatatgg cctgatgagc tttgtaaagg cctgccacga acgtggcatt
      421 ggcgtgattc ttgacgttgt atacaaccac ttttacggag aaagtgacct ctggcagttt
      481 gatggttggt gtgaaaataa tcgcggtggc atttactttt ataatgatac gcgaggcgat
      541 acaccttggg gcggtcgtcc agattatggc cggccagagg tgcggcaatt tatcttagat
      601 aacattacaa tgtggcttaa tgaatatcac cttgacgggc tacgtatcga ttcgacaatt
      661 tacatgcgca acactgaagg gcgcgataat gacccagaac atgatatccc tgacgcctgg
      721 tttttgctgc aggatatcgt agaggtggct cacaaaatta acccaaagtc gctcatcatt
      781 gcggaggact gtgccaacag ctcatatatt acctgcaatc gatctcagac tggttgcgga
      841 tttgatgcac agtgggagct tggattccct catgtactcc gcgatgggct cgggcttaat
      901 ccaaactcaa cgccaacatt aagtggtgtg cgctatgagc ttgagcgtaa ttataatggt
      961 gatgcatttg aaaaggttat tttcagcgac tcgcatgata ctgcagcgaa cggatctgta
     1021 cgacttaacg atgcaattac gccagaaaac ccaaccagcc tcgctgcaaa gcagaaaaca
     1081 ttacttgcag cctgtatgat gttcactgcg cctggcattc cgatgatgct ccaaggggca
     1141 gaatttcttc aagatggagc cttcaacgat tggcaagcac ttgaatggaa tcatcttacg
     1201 gcgcatgctg ggtttaccct tgcgcatcag cacctcaccg cacttcgtaa aaatatctac
     1261 agtaattctg ttgggctcca aggccaatac actgcagtat ttcaccaaga tgacaacaat
     1321 cacgttatag gatttgcacg cgcaactaac tctgatagta acgatagcac gatcgtaatt
     1381 gctaacttta gtaacttaaa tcttcgaaca taccagctcc atttgccagg gctcgggacc
     1441 tggaatgttc gattcaatag tacatggaaa ggctacagtc ctgatttccc agaagtggcg
     1501 ctccacgaaa ttataaccga caataccggc atggcaacga ttcaacttgc cccctactcg
     1561 gtttatatcg cctctaaaca aagctaaagg ccccgccgta cccacgcgaa tgtgcgcggg
     1621 tacggacgag gcctgaacga agcttctggt gactagggct gcattcccga ctagatgatc
     1681 gtggcgagct ctcgatcact gacggtaggc agaaggtttc gagctgccgc gagaagcctg
     1741 aagtagtgct tcttgcgcgc cttgacggca cgccgtatct ggcagagctc gtgcaggcgt
     1801 cggccccaca ctcgacgctc gcgccatgcc tggcgagcct ggcccgcgaa gctcctggat
     1861 cgggcaagct tggtatggcc ctttcggctt cgacctgagc ggaggtggcc ggtcgccaga
     1921 cgatactgct gagtgctcag tgcagcgtac gtcgttgcac gctgacgagc atcctgcagt
     1981 tctgccagga acaccgcctc cagcgctttg atttcaggca ggagggcttc acccttctgc
     2041 cagctcttct tggcagcctc aagctgcagc ttgaagagtt ccatcggtgt cggtgccagc
     2101 atcatctgct cgcactcctt cctctccatc tcactggact tcgtcatatt ccctagcgct
     2161 atgcgccgtg gacatgcaca tattttaaca ccctctgcta cagggcacaa tttacggtct
     2221 ctttatatcc agttctgcta ttactggcct atggtcgctt cctacatcgg aagaaatctt
     2281 atacgatact gcctttattc cttttgatcc aaatatccag tcaattttag caagtcgcca
     2341 tgctggcatc cactcgagac ccgattgttt cgcactgata gtccgccggt gaaggccgtc
     2401 gagatcatga aggtctgtcc ttttgaggag ttcagtaatc gccgtgcctc ttcccatctc
     2461 agatacgcga ctgcccattc ctcttagttg agtatgcgga aatagacgag cggcataata
     2521 cagcggccgc gcgcgtactg cacgtgcaaa tttactggag gcatgcatcg cgttgaaatc
     2581 accgagcaca atcaccggca atgaatcatt ttggtttact accttagcta cttctggcac
     2641 ttgctgtaaa cgaagcgctt cactacgatc atccagatga attcccacaa tgcggaggag
     2701 cgtgccatta tcgtcgacag tggcctcaat aaatggacgc tgggtattag taaagtgatg
     2761 gatcagatag ctttttagcg gaagacgtga caatagtgct gctccataag aacccaacca
     2821 cgaatggcca tacgaaaaaa agacgatttc atacccaagc gctcgcagcg ccataagcgt
     2881 tgcctcctca atggcatcgg tattgccgaa ctctgggata aatacgacat cagcattcag
     2941 ccgtttgatt gtagcgatag ccatttgcgg ggtcgcgcga cgtgtatttg atccggcgac
     3001 aaacatccct tcggcattcc acgtcaccac acgtagctgc tcaatcacat ccgttccttg
     3061 atttccttaa tcgtatcgcg cagctctgcg gcacgttcga at
//
LOCUS       DATIIX010000058         7551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_541813, whole genome shotgun sequence.
ACCESSION   DATIIX010000058 DATIIX010000000
VERSION     DATIIX010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7551
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541813"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VLF64_01690"
     CDS             complement(<1..217)
                     /locus_tag="VLF64_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HSW90689.1"
                     /translation="MPRIELNTEELQAEKESLGEFLAQPTAYTDPDFTKNNKRFTELE
                     GIIAKATELKIIERQLTEAKQLAGGSDE"
     gene            complement(308..628)
                     /locus_tag="VLF64_01695"
     CDS             complement(308..628)
                     /locus_tag="VLF64_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HSW90690.1"
                     /translation="MKSSIHPQDYRPVVFSDEIAGFAFLTQSTAQTTETIKWEDGNEY
                     PLIKVHISSASHPFFTGEEKIIDTEGRVDRFASRLKAAEERKAALAAKAKKAAAKKAA
                     KADR"
     gene            886..1587
                     /gene="rpsB"
                     /locus_tag="VLF64_01700"
     CDS             886..1587
                     /gene="rpsB"
                     /locus_tag="VLF64_01700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HSW90691.1"
                     /translation="MAVEVDIKALLEAGVHFGHKTSRWHPKMAPYIHSKRQHSHIIDL
                     TKTVEQLDIALPFITKVAASGKQVLFVGTKKQAKEAVKAAAEQVGQPYVTERWIGGML
                     TNVATVTAQIKKLKDLERRMASGDLEKRYNKLEVQRFQEEIDLLNSKYGGIKDLNGRP
                     GAIVVIDTLVDKNAIAEAKNLGVPVVGVVDTNADPSVVDYAIPGNDDAIKGTTLLLDY
                     FVAAVREGQTKKEEK"
     gene            1590..2186
                     /gene="tsf"
                     /locus_tag="VLF64_01705"
     CDS             1590..2186
                     /gene="tsf"
                     /locus_tag="VLF64_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HSW90692.1"
                     /translation="MGVSIEDIKKLKELTGIGLTDAKAALTEANGDFDKALEAMRKKG
                     LTKAEKKGDRETREGLVESYVHSNRIGVVVEVNCETDFVARLPEFKEFAHQIAMQIAA
                     MNPSYSTMEDIPAEVLEAKKIELLESESLKSKPAEMRDQIVEGQLKKHFAEQVLSEQA
                     FVLDDTRTVGERIKEQIALSGENIRVSQFRRIELGVTE"
     gene            2294..2848
                     /gene="frr"
                     /locus_tag="VLF64_01710"
     CDS             2294..2848
                     /gene="frr"
                     /locus_tag="VLF64_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HSW90693.1"
                     /translation="MFSTDDYELKMAGALEHFEAELSKIRTGRAHPGMLDGIKVEAYG
                     ALMPLNQTANITAPEAQLLQITPFDPSNIQAIAAAIRNDQSLGFNPSDDGRVVRVPVP
                     ALTEERRRQLVKQAGEKVEEVRIALRNIRQDGLKDAKAKKDAKEISEDDLKMIEKEFD
                     RLMGEQQVKIEQVFKAKEKDILTI"
     gene            2848..3534
                     /gene="uppS"
                     /locus_tag="VLF64_01715"
     CDS             2848..3534
                     /gene="uppS"
                     /locus_tag="VLF64_01715"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HSW90694.1"
                     /translation="MSEVPTHVGYILDGNRRWAKKHGLPTYEGHLAGYNALYDVIKAS
                     SDAGVEFISLYTFSAQNWSRAEEEVNGILKIIRRMFRGMLKRLIKDGHRVIVLGSREG
                     IPDDIVTLIEEAEASSKDGVRSTVAICFNYGGQEEIVRAARRLVERGVGVEDVTMGSV
                     AGALDHPEVPPCDLIVRTSGEQRLSDFMLWRAAYSEFIFLDKYWPEMTKEDVTSILEE
                     YTRRSRRFGG"
     gene            3535..4698
                     /locus_tag="VLF64_01720"
     CDS             3535..4698
                     /locus_tag="VLF64_01720"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HSW90695.1"
                     /translation="MNILLGVIVGIIVLTILVVAHELGHALVARRYGTVVEEFGVGFP
                     PMAWGKRVKKSILGKDVLLSVNWLPLGGFVKMQGEHDDDTGKGDYGAMTFWQKTQVLL
                     AGVVVNWLLAVVLITVLAFVGIPKIMPNQFSVSSDTHEISQPVELVGVTKDLPAAAAG
                     LKDGDQIIKIADRRVASPDDLAKLTADNKGKTVAIEYKREGAAHSVNVAIRSSNDDRK
                     GFLGVEPAQREVVRATWSAPIVGVCTTAQFTWLTLQGLGQLVGNTVSGLVMKLSPNHS
                     TQKQANANLAAAGSSVAGPLAIFGIIFPAADKMGLAYVIMIAAVISLTLAVMNVLPIP
                     ALDGGRWFVTALYKVLRKPLTKDVEERIHGTGFIVLMLLVIVISVVDIGKLHS"
     gene            4698..5453
                     /locus_tag="VLF64_01725"
     CDS             4698..5453
                     /locus_tag="VLF64_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HSW90696.1"
                     /translation="MDIAKPTSFATGKKPRLSTMLGGLAGWTTLLVAAYIGAQYIVSF
                     VIIALMHLGLPWSETSTVNILSYRVYVYVVMVLIIGAVAWYRYRELSFEQIGFQRLPE
                     WGDIGLSLAGAIVYMLASTIALTLASTIPAFSSDQTQDLGLGRLFGNDLIIGFFVLVV
                     LTPLFEEVIFRGFLYGRLRKLQFPWWVSSLVVSVLFGLAHMQWNVGVDVFFLSMVACI
                     LREMTDSIWAGVLLHMIKNFLAFLVTFVFIQGV"
     gene            5514..6758
                     /locus_tag="VLF64_01730"
     CDS             5514..6758
                     /locus_tag="VLF64_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HSW90697.1"
                     /translation="MRLSQNFTKTRKEAPADEQARNAQLLIRAGFVHKEMAGVYAFLP
                     LGKRVLDNIAQIVREEMNAIGGEEVQMTVLQPRELFERTDRWDDAKVDNWFKTKLVSG
                     AELGMGLTHEEPIVDAVGEFINSYKDLPIMVYQIQNKFRNELRAKSGLLRGREFVMKD
                     MYSFARSQEEHAEIYEKVALAYARVYDRLGVGAETYRTYADGGIFTEKFSDEFQTLSS
                     IGEDTIYVHEGKKIAVNKEIYTDENLAKLGLNKSELVEKRGVEVGNIFPLESKYTDAL
                     DVTYTDEDGNEQSIIMGCYGIGVSRLVGLLAEHFADDKGLVWPENIAPAKVYLVQIGQ
                     ESKETADALYDEFMSKGVEVIYDDRDERPGAKFADSELMGIPYRITVSDRLLSDDKFE
                     LTLRSTGVTELLTREALLAKLG"
     gene            6841..7296
                     /gene="greA"
                     /locus_tag="VLF64_01735"
     CDS             6841..7296
                     /gene="greA"
                     /locus_tag="VLF64_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HSW90698.1"
                     /translation="MKKTYQITEEGRKELEAELDELKGRRGEIASKIAEARDYGDLSE
                     NAEYDSAREEQGLVETRIAEIEDILVNAEIIKVKKSSKVHVGSTVEIKTGTKTVTYTI
                     VGPVEADPMNGKISNESPLGTALMDKEVGDKVMLTTPKGDTTYTVVSVN"
BASE COUNT         2003 a         1415 c         2058 g         2075 t
ORIGIN      
        1 gttcatcgct gccgccagcg agttgtttgg cctcggtaag ttgcctttcg atgattttga
       61 gttctgttgc ttttgcaata ataccttcca gctcagtgaa gcgcttgttg ttttttgtga
      121 aatctgggtc tgtgtaggcg gttggctgtg ccaaaaattc gccgagtgat tctttttcgg
      181 cttgaagttc ttcggtgttg agctcaattc ttggcataat cttcctttat tatactagta
      241 accctgtatt ctgtaatcct aacaaaaaga gactacctgt aaaacagcag tctctttaac
      301 gtggaagcta acggtcagct tttgcagcct tttttgctgc tgctttctta gccttagcgg
      361 caagagcagc tttgcgttct tcggcagcct taaggcgtga tgcgaagcga tcaacgcgac
      421 cttcggtgtc aatgattttt tcttcacccg taaagaatgg gtgagaggct gatgagatgt
      481 gtaccttgat aagtgggtat tcgttgccgt cctcccattt aattgtttcg gtagtttgcg
      541 ctgttgactg agtcaagaac gcgaagcccg cgatttcgtc gctaaatacg accgggcggt
      601 agtcctgtgg gtgaatactg cttttcatat tcttaatatt gtaactgata agtggtgaaa
      661 agtcaaaggg ttcatcgatc ttataacagt ccataaggaa gtggatgtaa gctcgacaat
      721 gtgcgaccag atgttattct cttataggct tattggtaca gtggtagacc ccttggcaag
      781 agggaaagat tttgctataa tagcgaagta ataactttta acgaaacaac tcttaccaag
      841 atatcctgtg catcaggcgg taagggtgaa gggagtaagg atatcatggc cgttgaggta
      901 gatatcaagg ctttgctcga agctggtgtt cactttggac acaaaacgag ccgttggcac
      961 ccaaagatgg cgccatacat tcatagtaag cgtcagcaca gtcacattat tgacctgact
     1021 aagacggttg agcaacttga tattgcatta ccattcatca ccaaggtagc ggcaagtggc
     1081 aagcaggttc tctttgttgg cactaagaag caggcgaaag aggcggttaa ggctgcagct
     1141 gagcaggttg gtcagccata tgtaactgag cgttggattg gcggtatgct tactaacgtt
     1201 gcaacggtta ctgcacagat caagaagctt aaggaccttg agcgccgtat ggcaagtggt
     1261 gaccttgaga aacgctataa taagcttgag gtacaacgtt ttcaggaaga gattgatctt
     1321 ttgaatagca agtatggcgg catcaaggat cttaatggtc gtcctggtgc cattgttgtt
     1381 atcgacacac ttgttgataa aaacgcaatt gctgaagcta agaaccttgg cgttccagtt
     1441 gttggtgttg ttgacacaaa cgctgatccg agtgttgttg actacgctat tcctggtaac
     1501 gatgatgcaa tcaagggcac tacgctcctc ttagactatt tcgtggccgc ggtccgtgaa
     1561 ggtcagacta agaaagagga gaaataaccg tgggcgtatc aatcgaagat attaagaagc
     1621 tcaaagagct tactggtatt ggcttgactg atgctaaggc cgcacttact gaagcgaatg
     1681 gtgactttga caaggcactt gaagctatgc gtaagaaagg ccttacaaag gctgaaaaga
     1741 agggcgaccg tgaaactcgt gagggtcttg tagaaagcta cgtacactca aaccgtattg
     1801 gtgtggttgt ggaagtaaac tgcgagaccg actttgttgc gcgtcttcct gagttcaagg
     1861 agttcgcaca ccagatcgct atgcagatcg ctgcaatgaa ccctagttac agcactatgg
     1921 aagatattcc tgccgaagtg cttgaggcga agaagattga acttctcgag agtgaaagtt
     1981 tgaaatctaa accagctgaa atgcgcgacc agattgttga aggtcagctc aaaaagcact
     2041 ttgctgagca ggttttatct gagcaggcat ttgtacttga tgatactagg actgttggtg
     2101 agcgcattaa ggagcaaatc gcactttcag gcgagaacat ccgcgtaagc cagttccgcc
     2161 gtattgagct tggtgttact gaataaatca ggtgccaaac tagagacaaa tagcccggga
     2221 gatcgggcta ttttgttggt aatacagaga ttcgttcaaa atgctataat agagctaaac
     2281 gaaagcgagg attatgttta gtactgatga ctatgagttg aaaatggcgg gtgctttaga
     2341 gcactttgaa gcagagttga gtaaaattcg cactggtcga gcacatcccg gtatgcttga
     2401 tggcattaag gttgaggcct atggtgcatt gatgccgctt aaccagactg caaatatcac
     2461 tgcacctgag gcgcagttgc tgcagattac cccgtttgac ccatcaaaca tccaggcaat
     2521 tgctgcggct attcgtaatg atcaatcact tggatttaac ccaagtgatg atggtcgcgt
     2581 agtgcgtgtg ccagtacctg cgctcaccga agagcgacgt cgccaactag taaagcaagc
     2641 gggcgaaaag gtagaagaag tgcgcatcgc acttcgtaat attcgtcaag atggcttgaa
     2701 agatgctaag gctaagaaag atgctaagga aatttctgag gacgacctga agatgattga
     2761 aaaagaattt gaccgtctga tgggtgaaca gcaagtgaag attgaacaag tgttcaaggc
     2821 caaagagaaa gatattctta caatctaatg tctgaagtgc cgactcatgt agggtatatt
     2881 cttgacggca atcgccgttg ggcaaagaag catggtttac caacttacga aggacacctt
     2941 gcgggatata atgcattgta tgacgtcatt aaagcgtcaa gtgatgctgg cgttgaattt
     3001 atatctctat atacattctc tgctcagaac tggtcgcggg ctgaggaaga ggtgaacggt
     3061 atcctgaaga taattcgccg aatgtttaga gggatgctta aacgtttgat taaagatgga
     3121 catcgggtga ttgtgctcgg ttctcgcgag ggaatccctg atgatattgt gacattgatt
     3181 gaggaggcgg aggcatcaag taaggatggc gtgcgatcaa ctgttgccat ctgctttaac
     3241 tatggcggtc aggaagaaat cgtacgagcc gcacgtcgtt tggttgaacg gggtgtcggt
     3301 gtcgaggatg taacaatggg atctgtggcg ggtgcgctcg accacccaga ggttccgcca
     3361 tgtgatttaa ttgtacgaac gagcggggag cagcggttga gcgattttat gctgtggcgt
     3421 gctgcgtaca gtgagtttat cttcttagac aaatactggc ccgagatgac aaaagaagac
     3481 gtaaccagta tactggaaga atatacacgg cgttcgcgtc gatttggagg atagatgaat
     3541 atattgctgg gggtaatcgt aggaattatt gttctgacga ttcttgtcgt tgctcacgaa
     3601 ttagggcatg cgcttgttgc gcggcgatat ggtacggtag ttgaagagtt tggtgttggc
     3661 tttccgccaa tggcctgggg taagagggta aaaaagagca ttcttggtaa ggatgtattg
     3721 cttagcgtca actggttacc gcttggtgga ttcgtgaaaa tgcagggtga gcacgacgat
     3781 gatacaggta agggcgatta tggtgcaatg acgttttggc agaaaacgca ggtactacta
     3841 gcgggtgtgg ttgtgaactg gttgctcgca gtagtgctca ttacggtttt ggcgtttgtg
     3901 ggaattccaa agattatgcc aaatcagttt tcagtctcct ctgacaccca tgagataagt
     3961 cagccagttg aacttgttgg cgtgacaaaa gatttgccgg ctgcagcggc gggtttgaag
     4021 gatggggatc agattattaa gattgcagat agacgggtag catcaccaga cgatcttgca
     4081 aagcttacag cggataacaa gggtaagacg gttgcgattg agtataagcg tgagggtgct
     4141 gctcatagtg ttaatgtggc aattcgtagt agtaatgatg atagaaaggg ctttttagga
     4201 gttgaacctg cccagcgtga agttgtgcgc gcaacgtggt cggcaccaat tgtgggtgtg
     4261 tgtactacag cacagtttac atggctaaca ctacaaggcc tcgggcaatt agtaggtaat
     4321 actgtatctg gtcttgttat gaaactcagt ccgaatcatt caacgcagaa gcaggcaaat
     4381 gcgaacttgg ccgcggcagg cagtagcgtg gctggcccac tcgcaatatt tgggattatt
     4441 ttccccgctg ctgacaagat gggtctggcc tatgtgatta tgattgccgc agtgatttca
     4501 ttgacgcttg ccgtgatgaa tgtgctgccg atccctgcac ttgatggcgg ccgctggttc
     4561 gtaacggcat tatataaggt gcttagaaaa ccgctcacaa aggatgttga agagcgtatt
     4621 catggtacgg gatttatcgt actcatgctt cttgtgatcg tgattagtgt cgttgacatt
     4681 ggaaagctac attcgtaatg gatatagcga aaccgacatc ttttgctaca ggcaagaaac
     4741 cgcgacttag cacaatgctg ggtgggcttg cgggttggac aacattgctt gttgctgcgt
     4801 atattggcgc acagtatatc gtgagttttg taattattgc gttaatgcat cttggtttgc
     4861 cgtggagtga gacttctacc gtgaatatac ttagctaccg cgtatatgta tatgttgtaa
     4921 tggtactgat tatcggtgca gtcgcttggt accgctacag agaactcagc tttgaacaga
     4981 ttgggtttca gcggttacca gagtggggtg atataggatt gagtctggcg ggtgcaatcg
     5041 tctatatgct tgcatcaacc atcgcgctaa cattggcatc tactatccca gcttttagtt
     5101 cggatcagac gcaagatttg ggtctcgggc gcttatttgg caatgattta atcatagggt
     5161 tttttgtgct cgttgtgcta actccattat ttgaggaagt aattttccgc ggatttttgt
     5221 atggccgttt acgcaagtta cagttccctt ggtgggtttc ttcgctcgtt gtgagtgttt
     5281 tatttggcct tgcacatatg cagtggaacg tgggtgttga tgtgtttttt ttgagtatgg
     5341 tggcgtgtat attacgtgaa atgactgata gtatttgggc gggcgtattg ctgcacatga
     5401 taaagaactt cttggcattt cttgtgacct ttgtatttat ccagggcgtg taatttgtag
     5461 tctatagtgt ctctctgttc aagagaggtg aaaacagtta caatagaggt attatgcgat
     5521 tatcacaaaa cttcaccaag acacgcaagg aagcgcctgc cgacgagcag gctaggaacg
     5581 cgcagctatt gattcgcgcg ggatttgtac acaaagaaat ggctggagtg tacgcttttt
     5641 tgccgctagg taagcgggtt cttgataaca tcgcgcagat cgttcgcgaa gaaatgaacg
     5701 ccatcggtgg cgaagaagtg cagatgacag tcttgcagcc acgtgaactt tttgagcgca
     5761 cggatcgctg ggatgacgct aaggtagaca actggtttaa gactaaactt gtcagtggcg
     5821 ctgaacttgg catgggcctt acgcatgaag agccgattgt tgatgcagtc ggtgagttta
     5881 tcaactcgta caaagattta ccgatcatgg tctaccagat tcagaacaaa ttccgcaatg
     5941 agcttcgcgc taagagtggt ttgctccgtg gccgagaatt tgtcatgaaa gatatgtact
     6001 catttgcacg cagccaggaa gagcatgctg agatctatga gaaagttgcg cttgcatacg
     6061 cacgagttta cgaccgtctt ggtgtcggcg ctgaaactta tcgaacgtat gcggatgggg
     6121 gtatttttac tgagaagttc agcgatgagt tccagaccct tagttcaatc ggtgaggaca
     6181 caatttatgt tcacgagggg aagaagattg cggtcaataa ggaaatttat actgatgaaa
     6241 atcttgcaaa actcggtctc aacaaatctg aacttgttga aaaacgcggt gtggaagtgg
     6301 gaaatatttt cccattagaa tcgaaatata cggatgcgtt ggacgttaca tatacagatg
     6361 aagacggcaa cgagcaatct attattatgg gctgctatgg tatcggggtt agccgactcg
     6421 ttgggctttt agcggagcat tttgctgatg ataagggctt agtgtggcct gagaatattg
     6481 cgccagcgaa ggtttattta gtgcagattg gtcaggaatc aaaagaaaca gcagacgccc
     6541 tttacgatga atttatgagc aagggtgttg aggttattta tgatgatcgc gatgagcgtc
     6601 caggcgctaa gtttgctgat agtgaactta tgggcattcc ataccgtatt acggtgagtg
     6661 atcgcttgct tagtgatgat aagtttgagc ttaccttgag gtcaacaggg gtgacagaac
     6721 tcttgacgcg tgaagcgtta cttgctaaac taggctagat tcagagccta tggaggggaa
     6781 taacaacgct aatcgaaatg attaggtccc gccatttggt ttggaagagg taaaaatatt
     6841 atgaagaaga cataccaaat cactgaagag gggcgcaaag agcttgaagc agagcttgat
     6901 gagcttaaag gccgacgcgg cgagattgcc agcaagattg cagaagctcg cgattacggg
     6961 gatcttagtg aaaacgctga atatgatagt gcccgcgaag agcagggtct tgttgaaact
     7021 cgtattgctg aaattgagga tatcttggtt aacgccgaaa ttatcaaggt gaagaaatca
     7081 agtaaggtcc acgttgggag cactgttgaa attaaaactg gtaccaagac agttacgtac
     7141 accattgttg ggccagttga agcagatcca atgaatggca agattagcaa cgaatctcca
     7201 cttggtactg cccttatgga taaggaagtt ggcgacaaag tcatgcttac cacgcctaaa
     7261 ggtgacacaa cgtacacggt tgtatcagta aactagtttt acttccgcat tggtatatac
     7321 tccgctctat gtaagagcgg agtattgctt ttggtggagg ttaacagtag tgactgtgat
     7381 acaatagtag ctagttatgg ctacattgaa agaactgcgc gatgaacgct tacgtaaatt
     7441 agaagagctg cgagctcttg gggtagatcc gtaccctgca gaggtgaatc gtacgcacaa
     7501 tattggtgat gttactactc aattcgagag ccttgaaggc agaacagtaa c
//
LOCUS       DATIIX010000059         3553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_321138, whole genome shotgun sequence.
ACCESSION   DATIIX010000059 DATIIX010000000
VERSION     DATIIX010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321138"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VLF64_01740"
     CDS             complement(<1..274)
                     /locus_tag="VLF64_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90699.1"
                     /translation="MFDKVLPLITTAKRIVIIQGENPDGDSLASSLALEEILSEQGKT
                     ITMYCAIDMPKYLRFLKGWDRVTNYFPFDADLAIIVDTAAEALIEKM"
     gene            complement(355..651)
                     /locus_tag="VLF64_01745"
     CDS             complement(355..651)
                     /locus_tag="VLF64_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90700.1"
                     /translation="MTHPDSLASVSIRFGLDDSVTITAGEVGGAAASGRNSVAYVQDA
                     DQTASTPQPDETSAYTVIAPAGTTVEFYQRGVDRMMEYVSGRKLVPIKVITISE"
     gene            complement(834..1448)
                     /gene="recR"
                     /locus_tag="VLF64_01750"
     CDS             complement(834..1448)
                     /gene="recR"
                     /locus_tag="VLF64_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HSW90701.1"
                     /translation="MVQLLPKALLAVIDELGRLPGVGVRTAERYAYYLLRSNTRNAYE
                     LAKAIAELHSGVKTCPVTFALIDAGENISPLYAGGGRNKKLIAVVEEPLDIIALERTG
                     QYHGTYHVLGGAISPIDGVGPEQLHVPELLKRLKDDEVEEVIIATNASVEGESTAIFM
                     QRQIEQTKLPVTVSRLARGIPVGVDLEYADQITLTHALEGRRIL"
     gene            complement(1461..1751)
                     /locus_tag="VLF64_01755"
     CDS             complement(1461..1751)
                     /locus_tag="VLF64_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HSW90702.1"
                     /translation="MAFDQVKMLNDLRKAQKDLAKEIVEVEAGDGAVVVQITGELKVK
                     SIKIDPEYVDLEDIHQLEHWIEIAIRDGMTKAQEIAAEKMKPLMGGLGKLGL"
     gene            complement(1795..3249)
                     /locus_tag="VLF64_01760"
     CDS             complement(1795..3249)
                     /locus_tag="VLF64_01760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSW90703.1"
                     /translation="MKFRVVDYTLYRWRYTIGYILLIILVLSALACAAFYVPGALRDG
                     EMQSALASGALSMHSIDPTTVVNLPYHILQRLSFLAFGVTILSIKLPSIILGLLTAFG
                     ILLLVKVWFRRNIAIIMVAVGATTAQFLFMVQDGTPAIMFNFVAIWLLVACTYMTRTR
                     IFSTFWKVAACVLTATALYIPLGIYIVLALFITASFHPHIRYMIRHIARVRLIIAILL
                     GLVALVPVGEAIMLNQKVALLLLGIPTSMNLQDNIISLGRDIFGFFLASDSYLLRPLY
                     SLSAVILMAVGAYRFMTVKYTARGYTVFFLSVFLVPLVIINPEHVTDLYVLAMLLMSM
                     GVNTLIATWYTLFPRNPYARIAGMIPISVFVVGMIFTGFASFVNNYSYNANVLQAYSN
                     DLKLLARSVVANHQQDTKLQVVTTKAQEPFYMLVAHYDNRFTVDTDFTTEQTVIMTHD
                     AYHNHAPNAAISSIITNRRATDADRFYVYKSVAK"
     gene            complement(3305..>3553)
                     /locus_tag="VLF64_01765"
     CDS             complement(3305..>3553)
                     /locus_tag="VLF64_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HSW90704.1"
                     /translation="RTPPIPQLADLRESGSIEQDADIVSFIYRPAYYEPDNPEVQNIT
                     DLIIAKHRNGPVGKVQLYFHPERLRFMSLDRRPDDEMM"
BASE COUNT          895 a          799 c          920 g          939 t
ORIGIN      
        1 gcattttttc aattaaggct tcagctgcag tgtcgacaat gattgcgaga tcggcatcaa
       61 atggaaagta atttgttact ctatcccagc cctttagaaa gcgcagatat tttggcatgt
      121 caatcgcgca gtacattgta atggttttgc cttgctcgct caggatctct tcaagtgcga
      181 gtgaacttgc gagtgagtcg ccatctgggt tttcaccctg aataataacg atacgttttg
      241 ctgtggtaat aagaggaagt actttatcaa acattgtgta ctattataac agataaagac
      301 aatgccccgt accacagcct cacgaagagg ccggtggtac ggggcacggt cacatcactc
      361 agatatggtg atcaccttga ttgggaccag cttcctgccg gagacgtatt ccatcatccg
      421 gtcgactcct cgctggtaga actcgaccgt tgtgcccgcc ggagcgatca ctgtgtaggc
      481 ggaggtctcg tcaggctgcg gagtcgacgc ggtttggtct gcgtcttgga cgtaggcgac
      541 gctgttacgg ccgctggcgg cggcgcctcc gacctcgcca gcagtgatcg tgactgagtc
      601 gtccagacca aacctaatgc tgacgctggc gaggctgtcg gggtgggtca tggcccagac
      661 tctaccaggc gaacctgccc tgagcgacac accgcgaggg tctaggaaga ccttcacgcg
      721 tgtgccctcg gcaagcggac atgctacagc gcccatggcg cacactcctt actccttgta
      781 tcgtgattga ccttgaggct attatggtac aattttctag aaaagcaagt agtttataga
      841 attctgcgtc cttcgagggc gtgtgtgagt gtgatttgat cggcatactc aagatcgacg
      901 ccaacaggaa tgccacgtgc gagacgtgag acggtcacag gcagtttggt ttgctcaatc
      961 tggcgctgca taaagattgc cgttgactcg ccctccacgc tggcattggt ggcgatgatt
     1021 acctcttcta cttcgtcgtc tttgaggcgt tttaataatt ctggcacatg taattgttct
     1081 ggcccaacgc catcgattgg tgagatggct ccgccaagta catggtatgt gccgtggtat
     1141 tggccagtgc gctcaagagc gataatgtca agaggctctt caacaacagc aataagcttt
     1201 ttattacggc cacccccagc gtagagtggt gatatattct cgcctgcgtc aataagcgcg
     1261 aaagttacag ggcaggtttt tacgccactg tgtagctccg caattgcctt ggcgagctca
     1321 taggcgttcc gagtatttga tcgcagtaaa taatatgcat atcgttctgc ggtgcgcacg
     1381 ccaacacctg gtagtcggcc aagctcgtca atgactgcca gtaaggcctt cggtaatagt
     1441 tgcaccatcg ttatttgctt ttagaggccg agtttaccga ggccgcccat aagtggtttc
     1501 attttttcag cagcaatttc ttgtgctttt gtcataccgt cacggattgc aatttcaatc
     1561 caatgctcaa gctggtgaat gtcttcaagg tcaacgtact ctgggtcaat cttgattgat
     1621 ttcactttga gctcgccggt gatttgtacg acaactgcgc cgtcacctgc ttcaacttca
     1681 acaatttcct ttgcaagatc tttttgtgcc ttgcgtaggt cgttcaacat cttcacttgg
     1741 tcaaatgcca ttatcaataa tcctccgtag ataactaact ttgtttagta tacgctattt
     1801 tgcgacagat ttgtagacat agaaccggtc ggcgtcggtt gcgcggcgat ttgtaataat
     1861 tgagctgatg gctgcgttcg gtgcgtggtt gtgatatgcg tcatgcgtca taatcactgt
     1921 ttgctcggtc gtgaagtcag tatcaacggt aaatcggtta tcgtagtggg ctacgagcat
     1981 atagaaaggc tcctgagctt ttgtggtgac aacttggagt tttgtatctt gctgatggtt
     2041 tgcgactacg ctgcgggcta atagctttag gtcattgcta tacgcttgta gtacatttgc
     2101 gttgtagctg taattgttca cgaagcttgc aaatccagtg aagatcatac ctacaacgaa
     2161 gacagagatt ggtatcatac cagcgatacg tgcatatggg ttgcgtggaa acaatgtata
     2221 ccacgttgca ataagtgtat ttacgcccat tgacatgagt agcatcgcaa gtacgtagag
     2281 gtcagtaacg tgttcgggat taatgataac aagcggtaca aggaatacac tcaggaagaa
     2341 tacggtatag ccgcgtgctg tatatttgac tgtcatgaat cggtaagctc cgaccgccat
     2401 caaaatgacc gcactaagtg aatatagcgg acgcagcaaa taactatctg aggctaagaa
     2461 gaacccaaat atatccctac caagcgagat tatgttgtct tgtagattca ttgaggttgg
     2521 gattccaagc aataatagcg cgactttttg gttgagcatg attgcttcgc caaccggcac
     2581 taatgcaaca agcccgagca gtatggcaat gattagtcgt acacgtgcaa tgtgacgaat
     2641 catatagcga atgtgtgggt ggaacgaagc ggtaataaag agtgcgagaa caatatagat
     2701 gccaagcgga atgtagagcg ctgttgcggt gagcacgcat gcagctactt tccagaaggt
     2761 actgaagatc cgtgtgcggg tcatatacgt acaggcaaca agtagccata tggcgacgaa
     2821 attaaacatt atcgccggcg taccatcttg taccataaag aggaactgtg cggtcgttgc
     2881 cccgactgcg accataataa tagcaatgtt gcggcggaac catactttca caagcagtaa
     2941 aattccgaat gccgtaagaa gcccgaggat gattgacggt aattttatac ttaggatggt
     3001 gacgccaaat gccaagaagc taaggcgctg tagaatgtgg tacggcaaat ttacgacagt
     3061 cgttgggtca atcgaatgca tcgataatgc accactcgca agtgcagact gcatttcccc
     3121 atcacgaagc gcacctggca catagaatgc agcacatgct agtgcactca aaacaagtat
     3181 gatcagcagt atgtagccga tagtgtagcg ccatctgtaa agcgtgtagt cgacaacgcg
     3241 aaatttcatt cttcaattat agcacgaggt gctgatgaaa tatgcttatg tttattactg
     3301 aaaactacat catctcatcg tcggggcgac gatcgaggct catgaagcga aggcgctctg
     3361 ggtggaagta gagctgcact ttaccaacag gaccgttacg gtgtttagcg atgatgaggt
     3421 cggtaatgtt ctgtacttcg gggttgtctg gttcgtagta cgcagggcga taaatgaaac
     3481 tcacgatatc tgcatcctgt tcgatggaac cggattcgcg aagatcggca agctgaggaa
     3541 ttggtggcgt acg
//
LOCUS       DATIIX010000060         2155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_541932, whole genome shotgun sequence.
ACCESSION   DATIIX010000060 DATIIX010000000
VERSION     DATIIX010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541932"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2130
                     /locus_tag="VLF64_01770"
     CDS             <1..2130
                     /locus_tag="VLF64_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90705.1"
                     /translation="RLWWAGEGDKGLGKDGSTIQMSVSHMTAGGSFHGSSHVSWAQAA
                     HDGKLKLAVSASRDTQTHVYFVDVRPNGEINIPQDDPSRKFFSIGKGGQVEFNGAYAE
                     VVEVRGEVGGVTHIAPLATEVGSNSLHEIPDKISVPTQHYVPHVKLTPPVITHEIPGQ
                     ALEVEGFGGPAIVHRRPLEALSRRRVEEQPIETGGYNSYRQRANGEYGRPEGADTISP
                     RLEANPTGQLVLGEELEWFAQELENSEGADYMRRIDSDIQNSAELQSLPAGLRTITTI
                     PVAAASESDNIYKTLSLYAQQDAEQLQHNAILLNVNWIDTVGNDPAKRAKIDKTFQEI
                     ERARRDFPGLHIATMTREYNGEEVKKTGGVIGYVARDLLNTALIAVHKRIQAGDIPFD
                     ADVAIVRQDADMRGLSRHYLRQLEGGMDKNPNVDIFHGTIRSDVPMQERYPGLGIVTN
                     FSQAMSAANAAANRPWTVGINAVVRASSMAAVNGLGRPTWTGAGSDDVNIAWRVGGAR
                     RGTPVVSPDSSTGYYQPQGLMDVKGHRIVVPIPGMSVDSSADRLIPQYLMGRHFGAAW
                     DSRVSQGTSFSDGPGGYRDRTADADIMKKVKREKVNDDFYDRIETNISGELQHANEEV
                     ARKILAIFFAGTPNAYTITGNIGDGSTKFELTDAGREFIKRSVQREVNGSRGAGYGAR
                     KMRQLYGQKRGGRRSVARESPFVAPLE"
BASE COUNT          541 a          450 c          614 g          550 t
ORIGIN      
        1 cgtctgtggt gggcgggtga aggagataaa ggcttaggga aagacggtag cactatccaa
       61 atgagtgtct ctcacatgac cgctggggga tcgttccatg ggagtagtca tgtttcgtgg
      121 gcgcaagctg cgcatgatgg caaactgaaa ctcgccgtgt cagcatctcg tgacacacag
      181 acacatgttt actttgtcga tgtcaggcca aatggggaga ttaacattcc ccaggacgat
      241 ccttcgcgga agtttttctc gataggtaag ggcggtcaag tagaatttaa tggtgcatat
      301 gctgaagttg ttgaggtccg tggagaggtt ggcggggtga cacatattgc accattagcc
      361 acggaagttg ggagtaactc actccatgag attccggata agattagtgt accgacacag
      421 cactatgttc ctcatgtgaa gttgacgcca ccagtgatta cacacgagat accgggtcaa
      481 gcacttgaag ttgaggggtt tggtgggcct gcaattgtac atcgccgacc gctcgaagct
      541 ttatcgcgtc gtcgtgttga agagcagcca atcgagacag gcggctataa cagttatagg
      601 cagagggcta atggtgaata tggtcgacct gagggtgccg ataccatatc gccaaggctc
      661 gaggccaatc caacaggtca actcgttctc ggcgaggaac ttgagtggtt tgctcaagag
      721 cttgaaaact ctgaaggcgc tgattatatg cggcgtattg atagtgatat tcagaactca
      781 gcagagcttc agtcgcttcc tgctgggttg cgtacaatca ctactattcc agtggctgcg
      841 gcaagcgaga gcgataatat ctacaagact ctttccttat atgctcaaca ggatgctgag
      901 cagctacagc acaacgctat tctgctaaat gtaaactgga ttgatacggt gggcaacgat
      961 cctgcgaaac gtgcaaagat tgataagaca ttccaggaaa tcgagcgtgc acgtcgtgac
     1021 ttcccggggt tgcatattgc aacaatgact cgtgagtaca atggcgagga agtaaagaag
     1081 acgggtggtg ttattggata tgttgcacgt gatcttttga atacggctct cattgcagta
     1141 cataagcgga ttcaagccgg cgatataccg tttgatgcag atgttgctat tgtgcgacaa
     1201 gatgcagata tgcgcggcct ctcccgtcac tatcttagac agcttgaagg cggaatggat
     1261 aaaaatccta atgttgatat tttccacggc actattcgtt cggatgttcc tatgcaagag
     1321 cgctatccgg gcctcggtat tgtgacgaac tttagccagg cgatgagtgc tgcaaatgcc
     1381 gctgcaaatc gtccttggac agtcggtatt aatgccgtcg ttcgtgcgtc atctatggct
     1441 gccgttaatg ggcttggtcg gcctacatgg actggtgcgg gttctgatga cgtaaacatt
     1501 gcatggcgag ttggcggtgc ccgccgaggt actccagtag tgtcgcccga ctctagtact
     1561 ggttactatc agccgcaagg acttatggac gtaaagggtc atcgtattgt tgttcctatt
     1621 cctggtatgt cggtagacag ttccgcagat aggcttatac ctcagtatct tatgggtcgt
     1681 cacttcggag cagcatggga ttcacgcgtt agtcagggca caagtttttc agacggacct
     1741 ggtgggtatc gtgataggac ggctgatgcc gatatcatga agaaggttaa gcgagagaaa
     1801 gtcaacgatg atttctatga ccgtatcgaa accaatattt caggtgagtt gcagcatgcg
     1861 aatgaagagg tcgcgcgcaa gattcttgct atcttctttg cgggaactcc aaatgcgtat
     1921 actattactg gtaatattgg tgatggatca actaaatttg aacttactga tgcaggccgc
     1981 gagtttatta aacgaagcgt tcagagggag gtcaatggat ctcgtggtgc aggttatggt
     2041 gcgcgtaaga tgaggcaact atatggccaa aaacgcggcg gtaggcgttc ggtggctcgg
     2101 gaatcgccgt ttgtggcacc gctggaataa caggttagta ataaatagga ggcaa
//
LOCUS       DATIIX010000061         5655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_515840, whole genome shotgun sequence.
ACCESSION   DATIIX010000061 DATIIX010000000
VERSION     DATIIX010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515840"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VLF64_01775"
     CDS             complement(<1..798)
                     /locus_tag="VLF64_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90706.1"
                     /translation="MDYTDFHLELIPATIVIFGVTGDLSTRKLLPSLYELEKNGLLHP
                     KTRIIGITRRKFTLAQLLKSSKEGIRDKDGRVIGSTLKSLGKKIEMYEMNAASGEGYS
                     GLKKYLDEIEAQVGLCMNRLYYLAIPPQVSAPIITHLGENGLNHGCTRHNTLSHLLLE
                     KPFGFDTATAEELITVTRKYFGEKQLFRIDHYLAKETVQNLVTFRFRNPIFEDIWDSK
                     HIKSISVIADEKIDIEGRANFYEQVGAMRDLIQSHLLNVVSVVMMNRP"
     gene            complement(800..1708)
                     /locus_tag="VLF64_01780"
     CDS             complement(800..1708)
                     /locus_tag="VLF64_01780"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF007161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HSW90707.1"
                     /translation="MKLILVGLGKMGGQIAKRLIESGHDVVAVDPNPAAVTAAAEFGA
                     ITSMSRIDAVSKFNGERIVVWLMIPAAYVNDEIAEWCKVLPDEAILIDGGNTDFRETK
                     KHALLASERNIHFLDIGVSGGIMGLANGFSMMGGGDGAAYEMITPILNALSAPRGGHA
                     YFGEVGSGHYVKMVHNAIEYGMMEALAEGYQMLREGPYKDLDLAKAGEVWQKASVIES
                     TLNELATEIMHEDKTLASASGYVAESGEARWTLEEAERAHITLPVIQAAFDVRLASEK
                     GDVSYTTKLLAELRNKFGGHPLNTNG"
     gene            complement(1718..2380)
                     /locus_tag="VLF64_01785"
     CDS             complement(1718..2380)
                     /locus_tag="VLF64_01785"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HSW90708.1"
                     /translation="MQLVMKTTDLGEKLADALTSALQMNDKVIWLVPGGSNIDISVQA
                     MQLLDDELTRKLVIMQTDERFVALDGEDCNWRQLRDAGFNVKRAEAFPILVDGESRDA
                     TVERYSETVLREFIAADYIIGQFGVGPDAHVAGIKPNSPASTSDALVCGYQGEDFERV
                     TLTFVALQQVDEALAFVFGEAKRPVLERLLGDIPSFAEFPAGVLRTSPISTIYNDQIE
                     DN"
     gene            2494..3408
                     /locus_tag="VLF64_01790"
     CDS             2494..3408
                     /locus_tag="VLF64_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90709.1"
                     /translation="MNSLPSLDTVVLGALEMLTTETVPTLDVTNFKRPLVVGSGNAEA
                     TGRILFAETDAIFASESSVDDKLSHIDDIDEVVVVSASGGKHAPILARKARDAGKHVT
                     LITNTPNSPAQRELSDSEITALVYPKNREPYTYNTSTYMSMMMGLTHEDPAAIKRFIE
                     EQIDTLELPDLSQFDKYYLIVPPKFADVIRMLHVKFIELFGRNIARDVETSEYVRHAT
                     TVAPSNNELFISFGEQNATWGNTNNRLNVPLPKDANYIAMVAVGYYVIAQIQKAHPQY
                     FKENINAYCQFISGVFGESIMPIVDGTV"
     gene            complement(3478..3888)
                     /locus_tag="VLF64_01795"
     CDS             complement(3478..3888)
                     /locus_tag="VLF64_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90710.1"
                     /translation="MSTELVLQHATSERWKELLDKFAEDAANKKREFRLPVELVIEAV
                     NGERWVMPAGTKVHLFAFITGAGATRHVFRTEGTFFMEDRDISICGAYPKRRVMSPKR
                     KPHRPVRPRCVRITLHDPMPSLGLRSERMPLNLI"
     gene            4259..5215
                     /gene="corA"
                     /locus_tag="VLF64_01800"
     CDS             4259..5215
                     /gene="corA"
                     /locus_tag="VLF64_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HSW90711.1"
                     /translation="MKIITSIDETLIKELRAKQEFFWLDLVSPSVKQVEQLGSLLSLH
                     PLAVEDSINFKQRPKLDIYENSALLVLAGTVQNQTSPHSIEEIHCYISGDYIVTLHHK
                     AYEELEQLMQLLKDRSPIKSEEYYVYKVIDAIVDSYFPPLADLDKSINDLENAIVSGV
                     KDHQLEEIFRLKRLLLQLRKVVVPMRDVFARQIEAIASLSGLESDSHDYFRDVYDHLI
                     RITDELDSYHEMVGSCTDLYLSMIANRQGEISKQLTIIASIFLPLTFLTGFFGMNFGW
                     EVNHIQSLWNFVLFGIGIMIVSVIAMAWWFHHKGWVGHSANR"
     gene            5361..>5655
                     /locus_tag="VLF64_01805"
     CDS             5361..>5655
                     /locus_tag="VLF64_01805"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSW90712.1"
                     /translation="MRTFSSDTKTTATPAQAWKAWTDLPNWKRHDPTIESITLDKFEN
                     GAKAKLVNKKGPGAILILEDIVPGKSFTIRTNLPLCTMMSFHEVVPTEDGGARF"
BASE COUNT         1549 a         1435 c         1214 g         1457 t
ORIGIN      
        1 cgggcggttc atcatcacaa cagacacgac attcagcagg tgactctgga ttaagtcgcg
       61 catagcccca acctgttcgt aaaagtttgc tcgaccctca atatcaatct tttcatcagc
      121 gataacacta attgacttaa tgtgcttgct atcccaaata tcttcaaaaa ttggattcct
      181 aaagcgaaaa gtcacaagat tctgaacagt ttcttttgca aggtaatggt cgatacggaa
      241 aagctgcttc tcgccaaagt acttgcgagt tacggtaatg agctcttctg cagtagcagt
      301 atcaaatcca aacggcttct caagcaatag gtgcgagagt gtattgtgcc ttgtgcagcc
      361 gtggttgagt ccattttcgc caagatgggt aataatcggc gccgatacct gtggcggaat
      421 tgcaaggtag taaagccggt tcatacataa gccaacctgc gcctcaatct catccaggta
      481 ctttttaagc cccgaatagc cctcgcccga tgcggcgttc atctcataca tctcaatctt
      541 tttgcccagc gatttaagcg tgctgcctat aacgcgacca tctttatcac gaatgccttc
      601 tttagatgat ttcagaagct gcgccaacgt aaatttacgg cgcgtaattc caatgatgcg
      661 cgttttcggg tgtaacagtc cgttcttttc taattcatac agtgacggca agagcttgcg
      721 tgttgaaaga tcgcctgtaa caccaaaaat aacgattgtc gcaggtataa gttcaagatg
      781 aaagtcagtg tagtccatat tatccattcg tgttgagcgg gtgaccgcca aatttattcc
      841 gtagctcagc aagaagcttg gtagtatagc ttacgtcacc tttttcagaa gcaagccgta
      901 catcaaatgc agcttgaatt accggcaatg taatatgcgc ccgctccgcc tcttcaagcg
      961 tccaacgtgc ttcaccggat tctgccacat aaccactcgc actcgcgagc gttttgtctt
     1021 catgcataat ttcagtcgca agctcattaa gcgtgctctc aatcacgctg gctttctgcc
     1081 acacttcacc agccttcgcg aggtcgagat ctttgtatgg cccctcgcgg agcatctggt
     1141 aaccttcggc gagcgcctcc atcatgccgt attcgatagc attatgtacc atcttcacat
     1201 agtggcctga tcccacttct ccaaagtagg catggccacc tcgtggcgct gaaagtgcat
     1261 tgagaatcgg cgtaatcatt tcgtaggccg ccccatcacc accacccatc atcgagaatc
     1321 cattagcaag ccccataata ccgccgctta caccaatatc aagaaagtga atatttcttt
     1381 cacttgcaag cagcgcatgc ttcttggtct cacgaaaatc tgtattgcca ccatcaataa
     1441 ggattgcctc atctggaagc actttgcacc actcggcaat ttcgtcattc acatatgctg
     1501 cagggatcat cagccatact acaatacgtt cgccgttaaa tttgctcacg gcatcaatac
     1561 gactcatgct cgtgatagca ccaaattctg ccgcagcagt cacggctgct gggttcgggt
     1621 caacagccac aacatcgtga cccgattcaa tcagccgctt agcaatttga ccgcccattt
     1681 taccgagtcc gacaagaata agtttcatat atgcccctta attatcttca atttgatcat
     1741 tataaatagt actgattgga cttgtacgaa gtacgcctgc agggaattca gcaaatgacg
     1801 gaatatcacc aaggagtctc tctagcacag gtcgctttgc ttcaccaaaa acaaaagcca
     1861 gtgcctcatc aacttgctgc aacgccacaa aggtaagcgt tacgcgttca aagtcctcgc
     1921 cttgataccc gcacaccaac gcgtcactcg tacttgctgg cgaattgggc ttaatacctg
     1981 caacgtgggc atctggccca acaccaaact gaccaataat atagtcagct gcaatgaatt
     2041 cccgtaacac cgtctcgcta taacgctcga ccgttgcgtc acgactctca ccatctacta
     2101 gtatcgggaa agcctcagca cgcttaacat tgaaccctgc gtcgcgcaat tgacgccaat
     2161 tacaatcctc gccatcaagc gctacaaagc gttcatcggt ctgcataatc accagcttac
     2221 gtgtcagctc atcatcaagt aattgcatcg cttgtacgct aatatcgata ttgcttcccc
     2281 cgggcaccag ccagataact ttgtcgttca tctgtaaggc tgaggttaat gcatcagcca
     2341 gtttctcccc taaatctgtc gttttcatca ctaactgcat actctattta gtattgcata
     2401 ctcacccctg acgcgctagt gctttatgct attatatgag ggaaggagag tctgatggag
     2461 cagccaaata gggaaaacct caagaatttt aatatgaact cactgccaag cctcgacact
     2521 gttgtgctcg gtgcgctaga aatgctaacg accgaaaccg taccgacgct tgatgttaca
     2581 aactttaagc gaccgcttgt cgtaggttcg ggcaatgccg aagccacagg tcgtatcttg
     2641 tttgctgaaa cggatgcaat ctttgcaagt gaaagctcag ttgatgataa actaagccat
     2701 atcgatgaca tcgacgaagt agttgtagtg tcagcatctg gcggcaaaca tgcgccgatc
     2761 ctagcacgta aagcacgcga cgcaggcaag catgttacac tcattacaaa tacaccaaac
     2821 tcaccggctc agcgagaact gtctgatagc gagattacag cgcttgtcta tcctaaaaac
     2881 cgcgaaccat acacctataa cacaagcacc tacatgtcaa tgatgatggg actaacacat
     2941 gaagatcctg cggcaattaa gcgttttatt gaagagcaga tcgatacgct tgagttaccc
     3001 gatctttccc agtttgataa atactatcta attgtaccac ccaaatttgc tgatgtaatt
     3061 cgcatgcttc atgtaaagtt cattgaactt ttcggacgca atatcgcccg tgatgtagaa
     3121 acgagcgaat atgtgcgcca tgcgacaaca gtggcgccaa gcaacaatga actcttcatt
     3181 agcttcggcg agcaaaacgc aacatggggc aacacaaata accgcctaaa tgtcccattg
     3241 cctaaagatg ctaactacat agcgatggtg gcggttggct actacgttat agcgcagatc
     3301 caaaaggcac acccgcaata ctttaaagag aatatcaatg cttattgcca gtttatttct
     3361 ggagtattcg gtgaatcaat tatgccaatt gtcgatggca cggtatagta tagcgctgat
     3421 atgacaaacc ggcccttgcc acgcccgaat agggcatggt aggtggccgc taggtctcta
     3481 gatcaggttg agcggcatgc gctcagagcg taggcctagg gagggcattg ggtcgtgcag
     3541 ggtgatgcgt acgcacctgg gccgcaccgg ccggtgcggc tttctcttcg gcgacatcac
     3601 cctccgcttc ggatatgcac cgcagatgct gatgtctcga tcctccatga agaacgtgcc
     3661 ttccgtgcgg aacacgtgcc gcgtcgcacc agcgccggtg atgaaagcga acaagtgaac
     3721 cttggtgccg gcaggcatca cccatctttc cccattgacc gcttcgatga cgagttcgac
     3781 cggcagccga aactctcgct ttttgttcgc cgcgtcctcg gcaaatttgt cgagcagttc
     3841 tttccaccgc tcggacgtgg cgtgttgcag tacgagctca gttgacatgc tctacccctt
     3901 actgacttga gccttaggta tgttatataa cagaaactat aattattcaa tagtacactc
     3961 aatgaaaata cctctccctt ggcgtttccg tagaatacca gggaagagca ggaggctctg
     4021 ttgtaggtct taccgctgac tagtgacctc aagcatttat cgtgcatata cgaaacatgt
     4081 tgctggactt aatccataca ccatcagtcg agaggcttct acaaaactta cgattttgcg
     4141 aagtaaaatg ccctttcttt ggagccacca acccagtgtc ttataatggg cagtgtagtg
     4201 agggggttaa acaaataaca gatacacata ccccgtatca tcaggagaac taacaaatat
     4261 gaagattatc acttcgatcg atgagacact tatcaaggaa ctacgtgcta aacaagagtt
     4321 tttctggctc gacctcgtta gcccgagcgt caaacaggtt gagcaacttg gctccctgct
     4381 tagtttgcac ccccttgccg ttgaagacag catcaatttc aaacagcgcc caaaacttga
     4441 catctacgag aacagtgcgc ttctcgtgct ggcgggcaca gtccaaaacc aaacatctcc
     4501 ccactctata gaagaaatcc actgttatat cagcggcgac tacattgtta ctctacacca
     4561 taaggcctac gaagagctcg aacaacttat gcaactgctt aaagacagat ctcctataaa
     4621 gagtgaggaa tattatgtat ataaagtcat cgacgcaatc gttgacagct atttcccgcc
     4681 gctggctgat ttagataagt caattaatga cttagaaaat gccatcgtct ctggcgttaa
     4741 agaccatcag ttagaagaga tcttcaggtt aaagcgattg ctactacaat tacgtaaggt
     4801 tgtcgtgcca atgcgtgatg tatttgcccg acagattgaa gctattgcta gtctgtcggg
     4861 gctcgaatct gatagccacg actacttccg ggacgtgtat gaccacctta tccggattac
     4921 cgacgaatta gactcatatc atgagatggt aggcagttgc accgacttat atctatcgat
     4981 gattgcaaac cgacagggag agatatctaa gcaattgaca atcattgcct cgatattcct
     5041 gccgttgacg ttcctgacag ggttcttcgg catgaatttt ggctgggagg tcaatcatat
     5101 ccaaagctta tggaacttcg tcctcttcgg aattggcatt atgatcgtat cggtaattgc
     5161 gatggcctgg tggttccacc ataaaggttg ggtagggcat agcgccaatc ggtgaagtca
     5221 gtaaggattt aagtctctaa tcttttagct ataaaacacg cctcctttag ccatacactc
     5281 ggtatataaa agtcaccgta atgtacttgc aaatattttc gatataattt agtatgtaca
     5341 catattaaaa ggagtccaac atgagaacat tttcatcaga cactaagacc accgccaccc
     5401 cagctcaagc ctggaaggca tggacagact taccaaactg gaaacggcac gatcctacaa
     5461 ttgagtcaat tacacttgat aagttcgaaa atggcgccaa ggctaaactt gttaataaaa
     5521 aggggccggg tgcgatacta attcttgaag atattgtccc tggtaaaagc tttacaattc
     5581 gtacgaatct accgctttgt actatgatga gcttccatga agtggtccca acagaagatg
     5641 gtggcgcacg attca
//
LOCUS       DATIIX010000062         9978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_265691, whole genome shotgun sequence.
ACCESSION   DATIIX010000062 DATIIX010000000
VERSION     DATIIX010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 9978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9978
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265691"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1330)
                     /locus_tag="VLF64_01810"
     CDS             complement(98..1330)
                     /locus_tag="VLF64_01810"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90713.1"
                     /translation="MMNPLLNTDRPLIMHIDLNSCFATVEQQARPMLRGRPVAIVNRR
                     TEHTMIVTASYEAKARGVKLGMRLREAKKLCPDLVGIESDPQKYRYVYHKMMRIMGDY
                     SPHVTMKSIDEGVIDFSQAPASMRNRDLVEIGCEIKQRLRDEIGCWMKCNVGISTNRF
                     LAKTAASLHKPDGLDVLTSHNIRDVLGALKLQDLTGIAHHNELRLNAVGIYTPLDMLN
                     ANASSLHVAFKSILGEQWYQRLRGWEVDDIDYDLKSCGRQYVLERPGLSQQEKVARLH
                     ALVEGVGAKLRHSHKSARGVRIYARLYGGGAWQAHELTPLPFYSDAAIWLIARRLFLG
                     APSNINMISVTCYHLCDDDDAQLSLFGDELARERHITWAIDEINGRYGERTIHSGNTL
                     TMNDIVKTKIPFGSTRYM"
     gene            complement(1327..1590)
                     /locus_tag="VLF64_01815"
     CDS             complement(1327..1590)
                     /locus_tag="VLF64_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90714.1"
                     /translation="MDDEIFLNERVDVVARFGTGMNPCVPIKFRRTNGREIIVTEVGL
                     RTPVAAGRKTVHWFDVTDGEADYRLEFDSERLTWRLTLEADRL"
     gene            complement(1769..2245)
                     /locus_tag="VLF64_01820"
     CDS             complement(1769..2245)
                     /locus_tag="VLF64_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90715.1"
                     /translation="MQQLSGLENSLDMLFKVLPRLPKDWRHWLADNVWWLALIVVAFG
                     CLGILGLVPVVLGISIFTGIIAGPAAGALVIITGIVNMAVLVVSVVLEAMAITPLKAK
                     QKRGWDLLFLVSLVTFTGAVLNALITGNINHIFGLIIGTLVGLYVLFEIRMYFVHK"
     gene            2343..2419
                     /locus_tag="VLF64_01825"
     tRNA            2343..2419
                     /locus_tag="VLF64_01825"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2377..2379,aa:Met,seq:cat)
     gene            complement(2837..3970)
                     /locus_tag="VLF64_01830"
     CDS             complement(2837..3970)
                     /locus_tag="VLF64_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90716.1"
                     /translation="MNSEPERATNSPDENKQLGIDTAEPHVDTATGAAPTSEKLVSTI
                     MENQPSVQPMPPMVAPEVPQTHKTQADAVTLVLQWLTYAFWGWFALAMLWLSAVVYAF
                     LVSGSDSNDWTSIVSYPIASALVLLIVAAVVDFFYRRREPAKKEGFATAVMVIHTVIF
                     ALCGIGALVASVFAGINLVLSEAPSASEGIRAALFTALTMVPLYALLIARTTLVAKWR
                     RIPMLATIILSVGALVFIGIGVFGPVMKSVATRQDRQVVSALSSIVYAVNSYDQDNDK
                     LPESLDGLDFNTYDSTGTVTVKSLLTKGVMTYKPNTKSGTKPSAQDTYRSRTIHYYQL
                     CATFMNAQTSPYTSYSPSDYSESINAQYHKKGEQCYNVKTNTY"
     gene            complement(4091..4237)
                     /locus_tag="VLF64_01835"
     CDS             complement(4091..4237)
                     /locus_tag="VLF64_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90717.1"
                     /translation="MEATTDTQSANLSETFVAASWNVPRTIEQLRSFVSSREDALDVE
                     DFDF"
     gene            4386..5105
                     /locus_tag="VLF64_01840"
     CDS             4386..5105
                     /locus_tag="VLF64_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90718.1"
                     /translation="MDTLTAFSVITLAALIHASFQLGVSMVTLLSGHAVGKKLAAKRT
                     LRLVGAFLAGTLIMTALVISTLSYIAGIFFNHGATPMAWGTLAFAMIIIGLVVWICYY
                     RRGPGTLLWVPRGLAKLLSKRIHATQMSAEAFSLGATSVFTELLFTITPAVAAAFAII
                     SLPTSLQLIGAGVYILVASLSILLVVGFIGSGHNISRLQRWREANKRFLQFAGGSALI
                     VLAFFLYINVVVTPVVVARVG"
     gene            5102..5383
                     /locus_tag="VLF64_01845"
     CDS             5102..5383
                     /locus_tag="VLF64_01845"
                     /inference="COORDINATES: protein motif:HMM:NF015442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HSW90719.1"
                     /translation="MSALATVIKQGRRGTESFDRGKLYESVYAACLSVQSVDGLARDT
                     TECVCNILTDWLENKLEITSSDIRRQVAKALEPLHPDAAYLYKHHKQMI"
     gene            5395..6771
                     /locus_tag="VLF64_01850"
     CDS             5395..6771
                     /locus_tag="VLF64_01850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSW90720.1"
                     /translation="MAKKHTFDYDLIVIGSGAGGSAAATIAAREGKKVAIVEQSVFGG
                     DSPNFSDVPTKALLHAAQLYDEARHGARFGLRSNALGYNYPSLRAWKNLAVERTGAAG
                     NRKFYESQGIDTIEARAHFLSPHEISVNRKHITAEHFLIATGSEWVAPDIPGIEDVPY
                     LTPRTVLESIKPTKSIYIIGAGTVGVEIAQLLAIFGTKVYLAEIAARILPQEEEEVGE
                     LFERMLREGKGVTSLTQTRTVAVVKDGLGYRVTFLRAGVEKSVHVDEVLVATGRKPSV
                     DLGLENASVEYDPTGIKANEYLQTTARHIYAAGDVLGHSSHTHTALLESRIAAHNLFS
                     KVQLAPDYTATPRLTFTFPGVATVGFTEDDCIKRDLHVNIGLAPLNIIARSNTSDFRD
                     GFVKLTADKKGVLLGATVVSPHAAEVIHELALAVKYGLTARQVAETPHAFLSWSEAVR
                     VAAGKLIR"
     gene            complement(7078..8058)
                     /locus_tag="VLF64_01855"
     CDS             complement(7078..8058)
                     /locus_tag="VLF64_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90721.1"
                     /translation="MERRNIVFLGANDDPHFPSVKRHLGPEYPEPIIINLGSPECTIG
                     YEFVRGRDEPIITYKEQEIDPASIRSIWYRNGVTRWLWPDLKVGPGLLEYTLSSLQHQ
                     ADTMVTFLDSGAFWVSDQQAIKRAQNKPPQLKVANMLGLRTPETIFASDAERAAAFVK
                     RHGTVILKPMAHCAPGGLDQYSTLRAGDQIDFRGLVANPHIFQELIKPKVEVRVSIIG
                     NRVFTAKVGDRDEAATLATGKRDYREAFNTGLFDVEPYDLPQDITDRLVAFVRYYGTV
                     CGYADLIQSLDDGEWYFLENNPNGQWVFQDDETTEGIGRALAQLLMTGQV"
     gene            complement(8130..8291)
                     /locus_tag="VLF64_01860"
     CDS             complement(8130..8291)
                     /locus_tag="VLF64_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90722.1"
                     /translation="MDDTQVTTSSEMQQTPESEPVFGALNGLLTPATGPSATTVKGKT
                     TLTENNGNG"
     gene            complement(8647..8724)
                     /locus_tag="VLF64_01865"
     tRNA            complement(8647..8724)
                     /locus_tag="VLF64_01865"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8687..8689),aa:Met,seq:cat)
     gene            complement(8753..9205)
                     /locus_tag="VLF64_01870"
     CDS             complement(8753..9205)
                     /locus_tag="VLF64_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90723.1"
                     /translation="MFTAFYKRLHHYKRVYATLNNGVILIALIVAVSWVWGSISVMQT
                     NFSAQKIVDEQKRQLELTQLQVDTLKYEQNYYNSNEYKDLAARQNLGLVSPGEKVLIL
                     PPNSTAVRQQDMLDAKREVTTAQTSESTTNFEQWLEFFNGRAARGLQK"
     gene            9365..9440
                     /locus_tag="VLF64_01875"
     tRNA            9365..9440
                     /locus_tag="VLF64_01875"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9398..9400,aa:Ile,seq:cat)
     gene            complement(9489..9899)
                     /locus_tag="VLF64_01880"
     CDS             complement(9489..9899)
                     /locus_tag="VLF64_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90724.1"
                     /translation="MAEQRTDVAKTVLTPVQSADVIRYYDTAANRFAYDNGWKPKIAL
                     GRVAVNTTQPRHSIIPNEEKPERAFTEPALTPQEMSEGLEHLMKGVEPEPSRQSTGEW
                     PIDPTDEQWKRIEKLKETHDPITAYEMVMGIWAH"
BASE COUNT         2437 a         2517 c         2345 g         2679 t
ORIGIN      
        1 ctccattcct tagcatcgga gccataccgc caagcgtcat accgacaccg gccataatcg
       61 agataattgt ctttttactc atatatatag tataccccta catatagcgc gtacttccaa
      121 atgggatttt tgtcttcaca atatcgttca tggtgagcgt gttgcccgaa tgaatcgtcc
      181 gctcaccata gcgaccatta atttcgtcga tcgcccacgt aatatggcgc tcacgtgcaa
      241 gctcatcgcc gaagaggcta agctgagcgt cgtcgtcatc gcaaagatgg taacatgtca
      301 ccgatatcat attaatattg ctcggcgcgc ctaaaaacag ccggcgcgca atcaaccaga
      361 ttgccgcgtc gctataaaag ggcaggggag taagctcgtg cgcctgccat gcaccacccc
      421 catacaatct cgcatatatg cgcactcccc tcgctgattt atggctgtgc cgcagtttcg
      481 cgcccacccc ctctacgagc gcgtggagcc ttgctacctt ttcttgttgg cttagacctg
      541 gccgctcaag tacatactgt cgtccgcaac tttttaagtc ataatcaatg tcatcaactt
      601 cccagccgcg cagtcgctgg taccattgtt cacccaagat acttttaaat gccacgtgca
      661 gcgacgatgc gttggcgttt agcatatcaa gtggggtata gatacctaca gcattaagcc
      721 gtagttcgtt atggtgagcg atgcctgtta aatcttgcaa tttcaaggcc ccaagcacat
      781 cgcgtatatt gtgcgacgtt aacacatcta acccatctgg tttatgtaga cttgccgcag
      841 ttttagccaa aaaccgattc gtgctaatcc ctacattgca tttcatccag cagccgattt
      901 catcgcgcag ccgttgtttg atttcgcacc ctatctctac tagatcccga ttgcgcatcg
      961 aagctggtgc ttgactgaaa tctattaccc cctcatcaat acttttcatc gtaacgtgcg
     1021 gactgtaatc acccataatc cgcatcattt tgtggtagac atatcgatat ttttgcgggt
     1081 cgctttctat gccaacaagg tcggggcaaa gttttttcgc ttcacgcaac ctcataccta
     1141 gctttacgcc gcgtgcttta gcctcgtaac tcgctgtcac gatcatcgta tgttcggtgc
     1201 gtcgatttac aatcgcgact ggccgaccgc gaagcattgg cctcgcttgt tgctcgactg
     1261 ttgcgaaaca actgttcaag tcgatatgca tgattaatgg acggtcagta ttgagtaagg
     1321 gattcatcat aatcgatctg cctccaaagt aagccgccat gttagacgtt cgctatcgaa
     1381 ttcgagccgg taatctgcct cgccatcggt tacatcaaac cagtggactg ttttacggcc
     1441 agctgctact ggcgtacgga ggccaacttc agttacgatg atttcgcgac cattagtacg
     1501 ccgaaatttt attggtacac atggattcat tcccgtacca aagcgcgcca ctacatcaac
     1561 ccgttcgttt aaaaagattt catcgtccac agcaaaattc tccaaataag taattattga
     1621 agaaacgttt tctgattgct cgcccgtgct tttacccgtt tctagcaaaa atccgaagac
     1681 gatgaatatg atccgtggat gagggacgct tacactactt gtaggggtag ggcagtgagt
     1741 atcacctgga attaccttct attgtaccct atttgtgcac gaaatacatg cgtatctcaa
     1801 acagtacgta gagtccaaca agtgtgccga tgattagtcc gaaaatatga ttgatatttc
     1861 ctgtaatcag cgcattcaag acagcgccgg tgaaggtgac tagacttact aaaaatagca
     1921 agtcccatcc ccgcttttgc ttcgccttta acggtgtgat tgccattgct tcaagtacca
     1981 ccgacacaac aagcaccgcc atattcacga tacccgtaat gataacgagc gcacccgccg
     2041 ctgggcccgc aataatacca gtaaaaattg aaatgccgag cactaccggc acaagcccga
     2101 gaataccaag acacccgaat gcgacgacaa taagtgccag ccaccacacg ttatcggcaa
     2161 gccaatgccg ccagtctttt ggtaggcgtg gtagcacctt aaataacatg tctaggctgt
     2221 tctctaatcc cgataattgc tgcatattcc cctcctctat cttatgagta ttttaccatt
     2281 ttctataaac accctgatac cactagaaaa atacgttaac ttttgctaca atttacggga
     2341 atggcaccat agctcagctg gttagagcgc aggactcata agcctgaggt cggtagttca
     2401 agcctacctg gtgctaccat gaaaataagc ccacaacttg tgggcttatt ttcatggtag
     2461 ggctcaataa taaaaactac ctcgccgagg agcgtaagct cactcggcga ggtcttccag
     2521 ggtatgagag ggaaagagaa gcacttgcta gcccttctta cccttcttgt gcccctttcc
     2581 tggccgtcgc ggccactttt tcgacgtcat agcctcgcgt ttggtgcgct tgggtttgtc
     2641 gttcgccatg agccacctct taaggttctg caacagctga ggagctggtg cgacgaaaat
     2701 cggactgatg cccgattcaa gtattatact atagataatg gataattacg tcaaacacca
     2761 aagtacatat caccctaaag caaaaccgaa ataaaggcat cggtcatatg accgatgcct
     2821 ttatttcact agttccttag tatgtgtttg tcttcacgtt gtagcactgc tcaccttttt
     2881 tgtgatactg tgcattgatt gattcagaat agtcgctcgg gctatacgat gtatacggcg
     2941 atgtttgtgc attcataaat gttgcgcaaa gttggtagta atggatcgtg cgactcctat
     3001 aggtatcttg cgcggagggc tttgtgcctg atttggtatt tggtttatag gtcatcacgc
     3061 ctttcgtaag cagactctta acggtgactg taccagtact atcgtacgta ttaaaatcaa
     3121 gcccgtcgag tgattctgga agtttatcat tatcttgatc gtatgagttt actgcataca
     3181 cgatactact cagcgcgctg acaacttgcc gatcctgcct tgtagctacc gacttcatga
     3241 ccggaccaaa cacgccaata ccgataaaca cgagcgcacc tacgctcaaa ataatagtcg
     3301 caagcattgg aatgcgtcgc cattttgcta cgagtgtagt gcgtgcaatc aataatgcat
     3361 atagcggcac catcgtgagc gcagtaaaga gcgcggcgcg tataccttcg ctcgctgatg
     3421 gcgcttcact taacactaag ttaatacctg caaataccga tgcgacaagc gcaccgatgc
     3481 cgcagagtgc aaaaataacc gtgtgaatta ccatcactgc cgttgcaaaa ccttcttttt
     3541 tcgcaggctc acgacgtcga tagaagaaat caactacggc ggcaacaatc aaaagtacga
     3601 gtgctgaggc gattggatat gaaacgatac tcgtccagtc attactatca ctgccggaca
     3661 caagaaaggc gtacacgacc gcacttagcc atagcatcgc aagtgcgaac cagccccaga
     3721 atgcataggt aagccactgc aatacaagtg ttacggcatc tgcttgggtc ttgtgagttt
     3781 gtggaacttc tggtgctacc attggtggca ttggctggac agatggctga ttttccatga
     3841 tagttgatac tagcttttcg gaagtaggcg cggcccccgt tgctgtatcc acatgtggtt
     3901 cagcagtatc tattcccaac tgcttgtttt catcaggcga gttagtcgcg cgttcaggtt
     3961 cgctattcac tagaatctcc ttatctatat agccagtata ctatagagct tacggctata
     4021 tacagcaaaa agtgaccaga caattgttta tcaggtcact ttccaggtgg agtttcggac
     4081 aagtctggaa ttaaaagtca aaatcttcaa catcaagcgc atcttcacgc gatgatacaa
     4141 atgaacgaag ttgctcaatt gtgcgtggta cgttccagct tgctgctaca aatgtttcgc
     4201 tgaggttcgc cgactgggtg tcagttgttg cttccatatt gttaattgcc tttttttgtt
     4261 tttattggct tatgtattta atatagcata aacactataa aaaagcaata gctttatgac
     4321 ctctttttga ctcgagtgag atgcttatgg tataaataac aggagaggaa gaaaaaatag
     4381 aaacaatgga tactcttacc gccttcagtg ttatcactct tgccgcactg attcatgcaa
     4441 gcttccagct cggcgtgagc atggtaacgc tgctctcagg ccatgcagtt ggaaaaaaac
     4501 tcgcagccaa gcgaacactc cgtcttgttg gtgcgtttct tgctggcacc ctcattatga
     4561 ctgcacttgt aatttctaca ctgagttata tcgctggcat cttcttcaac catggcgcta
     4621 caccaatggc ctggggtaca cttgcttttg caatgatcat tattggcctc gtcgtatgga
     4681 tttgttacta ccgccgaggg cctggcacgc tactgtgggt gcctcgcggc cttgctaagc
     4741 tcctcagtaa gcgtattcat gcgacccaaa tgagcgccga ggcattcagt ttgggcgcaa
     4801 caagtgtatt tactgagcta ttgtttacta ttactccggc ggtagctgca gcatttgcta
     4861 ttatttcctt accgactagc ctgcagctta ttggtgcggg cgtatacatc ttggttgcat
     4921 cattgagtat tttgctcgtt gtagggttta ttggtagcgg ccacaacatt agccgcctgc
     4981 aacgctggcg tgaggccaac aagcgtttct tacagtttgc tggcggctcg gcgcttattg
     5041 tcctcgcctt cttcctatac attaatgtcg ttgttacacc ggtagtcgtt gcgagggtgg
     5101 gatgagtgct cttgctactg ttattaagca aggtcgtcgc gggactgagt ccttcgatcg
     5161 aggaaaactc tacgagagtg tctatgccgc ttgcctcagt gtacagagtg ttgatggcct
     5221 tgccagagat accaccgaat gtgtttgtaa tattcttacc gattggcttg aaaataaact
     5281 agagattacg agtagtgata ttcgccgcca agtggccaag gcgcttgaac cactccatcc
     5341 agatgccgct tatttatata aacaccacaa acaaatgata taaggagccc ggatatggcc
     5401 aaaaaacaca ccttcgacta tgacctcatc gttatcggca gcggcgccgg aggatctgca
     5461 gctgcaacga ttgccgcgcg tgaaggtaag aaagtcgcaa ttgttgaaca gtcggtattt
     5521 ggtggcgact caccaaactt tagtgatgtg ccgacaaagg cactcctcca tgccgcacag
     5581 ctctacgacg aagctcgcca tggcgcgcgc tttggccttc gtagcaatgc gctcgggtac
     5641 aactatccgt cactccgcgc ctggaagaac ctcgctgttg agcggactgg ggctgcgggt
     5701 aatcgtaaat tctatgaatc acaaggaatc gatactattg aggcgcgcgc gcacttcttg
     5761 tctccgcacg aaatatctgt caatcgcaaa catattaccg ccgagcactt cctcattgcc
     5821 actggctcgg aatgggtcgc gccggacatc cccggtatcg aggacgtccc ttatcttaca
     5881 ccgcgtactg tgctcgaatc cattaagccg acgaagagta tttatattat tggcgctggt
     5941 actgtgggcg tagagatcgc acaactactt gcgatctttg gtacgaaagt gtacctcgct
     6001 gaaatcgccg ctcgtatttt accgcaagag gaagaagaag ttggcgaact atttgaacgt
     6061 atgctgcgcg aaggtaaagg ggtgacttcg ctcacacaga cgcgcaccgt tgctgtcgta
     6121 aaagatggtc ttggctaccg cgtgacattc ctgcgtgctg gtgttgagaa atctgttcac
     6181 gtagatgaag tactcgttgc aactggacgc aagccgtcag ttgatctcgg ccttgaaaac
     6241 gctagcgtgg agtatgatcc gacaggtatt aaggcaaatg aatacctaca gactaccgct
     6301 cggcacatct atgcagctgg cgacgtactc ggccacagca gccatacaca caccgcattg
     6361 ctcgaaagcc gcattgctgc acacaactta tttagcaaag ttcaacttgc tccggattac
     6421 accgcaacac cacgccttac ttttacgttc ccgggtgttg ccacggttgg ttttaccgaa
     6481 gacgattgta ttaagcgcga tttgcatgta aatattggac ttgcaccact aaatattatt
     6541 gctcgcagca acacgagtga cttccgcgac ggatttgtga aacttactgc cgataaaaag
     6601 ggtgtactac tcggcgcgac cgttgtgtca ccacacgcag ccgaggtgat tcacgagctt
     6661 gcacttgccg ttaagtacgg gcttaccgca cgccaagttg ccgagacacc ccacgcattc
     6721 cttagttgga gcgaagcggt gcgtgttgca gccggtaaat taatacgata agccctaggc
     6781 tatcaactaa aaaacgctcg tcccaagact agcgtttttt cttggttgcc caggtagctc
     6841 tgcgctggca ccagatctgt aataagatag tgtgttggag agaactaaca gatggtagac
     6901 ctctgttatt tagcactccc gcgtagtatt agatgagcgc caccaagccg aagagctatg
     6961 tagtgagaaa tatgaggctg aacaaataat aattactgac tgatcgcccc gacctcgcaa
     7021 caacgcgaag tcggggcgct tctaacgctt ttgagtgccg tcacgcatgg cgcgacgcta
     7081 cacttgacca gtcatcagaa gctgggcgag ggctctgcca attccctcag ttgtctcgtc
     7141 gtcctgaaac acccactggc cgttgggatt attttcgagg aagtaccact caccgtcgtc
     7201 aagactttgg atcaagtccg cgtaaccaca gacagtgccg tagtagcgaa caaaggccac
     7261 aagacgatcg gtgatgtcct gcggaaggtc gtacggttct acgtcgaaga ggccagtatt
     7321 gaaagcttcc cggtagtccc gcttccctgt cgcgagggtt gccgcctcat ctcgatcacc
     7381 aaccttggct gtgaaaaccc tgttgccgat aatggaaacc ctcacctcaa ccttgggctt
     7441 gatcagctcc tggaagatat gggggttggc gacgagtcca cggaagtcga tctggtcgcc
     7501 cgctcgaagg gtgctgtact ggtcgagacc acccggtgcg cagtgagcca tcggtttaag
     7561 gataacggtg ccgtgacgct tgacgaacgc ggctgcacgt tccgcatcgg atgcgaaaat
     7621 cgtttctggg gtcctgagac ctagcatgtt cgccactttc agttgtggcg gtttgttctg
     7681 agcccgcttg atcgcttgct gatcggaaac ccagaaggct ccactatcca gaaacgtcac
     7741 catggtatca gcctgatgct gcagcgagct gagtgtgtac tccaggagcc ccggcccaac
     7801 cttaaggtcg ggccaaagcc agcgagtcac cccatttctg taccaaatag accgaatgct
     7861 tgcaggatcg atctcctgtt ccttgtaggt gatgatcggc tcatcacgcc cccgcacgaa
     7921 ctcatatcca atcgtacact ctgggctgcc gagattgatg atgatcggct ccgggtattc
     7981 cgggcccaga tgacgcttca cactgggaaa gtgcgggtca tcatttgcgc ccaagaagac
     8041 gatattacga cgttccattt tacctccagg tgttgaacga gcccgtcatg ccggcacatt
     8101 gccggtatga cgggctcttt gatgggcgat cagccgttgc cgttgttctc ggtcagcgtc
     8161 gtcttgccct tgacggtcgt ggctgacggc ccggtcgccg gcgtcagcag cccgttgagg
     8221 gcgccgaaca cgggctccga ctcgggcgtt tgctgcattt cactgctggt ggtcacctgc
     8281 gtgtcgtcca tcagttcccc ttagtgaggt ggtctgcccg cggcggattg ccaacgtagc
     8341 gttttatatc atagtatgaa aagataattt ttgcaatggt cccattggac aactctcccg
     8401 acatagcaag taaatatatg aggcttattt gttatatggg tatagtcacc tcgtattgag
     8461 taacttttat ttgtaagctc tacttccatt aaagagttgc tatagaacta aagaaaaggt
     8521 cagaatgatt aatcggcttt aggctttttt ctttagctag gcaacaaaga tttaaaccaa
     8581 aataacagag gtgaaacaaa agatagagac tagcgtaaaa tctaatctct atctgttatt
     8641 tcatcttggt tgcgggggta ggatttgaac ctacgacctc ttggttatga gccaagcgag
     8701 ctgaccgggc tgctccaccc cgcgatactt ataccatcgt atcagatgag ggctactttt
     8761 gcaaacctct ggcagcacgc ccattaaaga actcgagcca ttgttcaaaa ttagttgttg
     8821 attcgctcgt ttgtgccgtt gtgacttcgc gttttgcgtc aagcatatct tgttgccgca
     8881 ccgctgtact attgggcggc aatattagta ctttttcgcc gggcgatact aaaccaagat
     8941 tctgacgagc tgcaaggtct ttgtactcat tgctattgta gtagttttgc tcgtacttta
     9001 gggtatccac ttgcaactgc gtaagttcaa gctggcgctt ttgctcatcc acaatctttt
     9061 gcgcggagaa gttggtttgc atcactgaga tcgagcccca tacccaactc actgcaacga
     9121 ttagcgcaat aagaatgacg ccattattga gcgtggcata gacacgtttg tagtgatgca
     9181 gtcgtttgta gaaagccgta aacatgatat ctggagtcta ttatagcagc gcaaggtact
     9241 catacgctat ggagtatagt atagctgttc gaaacttgat atattgcttc gtcacctcag
     9301 aaaaccccac aaacaggttt ttcgctcggc gccttgtggt ataataccct cttagaagta
     9361 ccctgggggt gtagctcagt ggttagagca gcgggctcat aacctgttgg tcgtcggttc
     9421 aatcccaacc acccccacca tattttgctt ctaaaaaatc tcacctgtta tggtgagatt
     9481 tttgtttatt agtgagccca gatgcccatt accatttcgt atgcagtgat agggtcatgt
     9541 gtttctttca gtttctcgat gcgtttccat tgttcgtcag ttggatcaat cggccattca
     9601 cctgtagact gtcgggacgg ttctggttct acacctttca taaggtgctc aagcccttcc
     9661 gacatctcct gcggagtaag cgccggctcc gtaaatgcac gctcaggctt ttcctcgtta
     9721 ggaatgatgc tgtgtcgtgg ttgcgtcgta ttaacagcta cacgaccaag agcaatttta
     9781 ggcttccacc cattgtcata ggcgaatctg tttgctgcag tatcgtaata gcggataaca
     9841 tccgccgact gcaccggtgt aaggactgtt tttgccacat ctgtgcgttg ttctgccatg
     9901 tttacgttat aacataaaca ctggcaatta tacacttggc attaccgctc acccctgcta
     9961 caccagaggt gtgggaag
//
LOCUS       DATIIX010000063         1594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_155025, whole genome shotgun sequence.
ACCESSION   DATIIX010000063 DATIIX010000000
VERSION     DATIIX010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155025"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VLF64_01885"
     CDS             complement(<1..200)
                     /locus_tag="VLF64_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90725.1"
                     /translation="MTHESRKIIGRADRIDLPELGFLGVPAKVDTGAKTSAIWASNIV
                     ERDGGLAFTLFDESYTLYTGEE"
     gene            408..692
                     /locus_tag="VLF64_01890"
     CDS             408..692
                     /locus_tag="VLF64_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90726.1"
                     /translation="MTASRETYLTTFARTCEQANRSGFSLNLLTGTPGERVDDDFSVT
                     FGVGGLVALTISNTLYEELKADLTPHMRKILRDFPHGQGQRKIEFYPHAT"
     gene            complement(744..>1594)
                     /locus_tag="VLF64_01895"
     CDS             complement(744..>1594)
                     /locus_tag="VLF64_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90727.1"
                     /translation="TDLARELEGINWDAKQIKQCVETVLEKWSVLSGHTVTWQEVDGR
                     DGFAPDGKFQVIITPRRKNMSVDSTRRLVNIPEGTVRPLVGLYPAGALPLIAHELSHV
                     LQAYADYELGQQIPLARIKGRRYRILREAGGAYQEKILCRDYLGLDRAPNLHYLIGYT
                     AKVNGSTRLQVARAFYESLIQGKSLTNDEDLAARTLAVNRSSRLYRYGGHSSQALDYV
                     EQSVVCGVLMERLSPDQVDAFLLGSTSFSLEDSALLHRFGLLTLPARAAYSPAQDVMR
                     VFESLI"
BASE COUNT          370 a          457 c          417 g          350 t
ORIGIN      
        1 atttcttcgc cggtgtacaa tgtgtagctt tcgtcgaaaa gcgtgaaggc caaaccgcca
       61 tcacgctcga cgatgttgct agcccaaatc gctgacgtct tcgcgccagt gtctaccttg
      121 gcgggcacgc ccaagaagcc cagttcaggc aggtctatac ggtcggcgcg gccaatgatt
      181 ttacgagatt catgagtcat agttgttacc acctattgta gcagctttgg agtttacccc
      241 acctcggctg actgaatgtt tttaggtagc gtaagcataa tacaaaaaga cgatttagtc
      301 aatacaaaac actatctact ttctccgtct catctgaagt aactattgaa aaagcataag
      361 ttttatggta taagtattaa gtcacccgta cctagggaga gtgcatagtg acggcatcca
      421 gagaaaccta tctcaccacc ttcgcacgta cctgcgagca ggccaacagg tcgggcttca
      481 gcctgaacct cctgaccggc acgccgggcg agagggtcga cgacgacttc tccgtgacgt
      541 tcggcgtcgg tggcctcgtg gccctcacca tcagcaacac gctgtacgag gaactgaagg
      601 ccgacctgac cccacacatg cgcaagatcc ttcgggattt cccgcatggc caaggccagc
      661 gcaagatcga gttctacccg cacgcgacgt gaccgacggc ccgccacctt gaccggtggc
      721 ggggccgcac acccttactg aacttatata agagattcga acacacgcat aacatcttgc
      781 gctgggctgt atgcagctcg agccggcaag gtgagcaatc cgaagcgatg caacaaggca
      841 ctatcttcca agctgaagct ggtgctgcca agcaagaatg catcgacttg gtcggggctg
      901 aggcgctcca tcaacacacc gcagaccaca gattgttcaa cgtagtccag cgcctgcgag
      961 ctatgaccgc catagcggta caagcgtgac gaccggttaa cggcgagtgt ccgcgcggcg
     1021 aggtcttcgt cgttggtgag cgacttacct tggatgagtg actcgtagaa tgcacgcgca
     1081 acttgtaaac gagttgagcc gttgaccttt gccgtatagc cgatcaaata atgcaggttt
     1141 ggcgcacggt ccaagcccag ataatcccga cacaggatct tctcttgata cgctccgcca
     1201 gcttctcgca aaatgcgata acggcggcct tttatacgtg ccaacggtat ctgttgaccg
     1261 agttcgtagt cggcgtaagc ctgcaggaca tggctcagtt cgtgcgcgat cagcggcagc
     1321 gcaccggctg gatacagacc cacaagcggt cgaactgtac cctctggaat attcaccagg
     1381 cggcgagtgc tgtcgacgga catattcttg cggcgaggcg taatgataac ctggaacttc
     1441 ccgtccggtg cgaatccgtc gcgaccatcg acttcctgcc atgttaccgt gtggccgctc
     1501 aggacgctcc acttttccag cacagtttcg acgcactgct tgatctgttt ggcatcccaa
     1561 tttataccct cgagctcacg tgctaggtca gttg
//
LOCUS       DATIIX010000064        10129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_376983, whole genome shotgun sequence.
ACCESSION   DATIIX010000064 DATIIX010000000
VERSION     DATIIX010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 10129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10129
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376983"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /gene="dnaN"
                     /locus_tag="VLF64_01900"
     CDS             complement(<1..944)
                     /gene="dnaN"
                     /locus_tag="VLF64_01900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HSW90728.1"
                     /translation="MELSVTQENLARALSIVGRVASSRTGLPILNNVLLRTEGNRLLV
                     AATNLEIAATYYVGAKVTKQGELTLPARLVSEFIASLPKGTVDIMSKNTSMTIASGNF
                     SSNLTGVDASEFPELPSIEEKEAVAYTITTEDFKQAVNQTIITTSSDTSRPVLTGVYW
                     HSIDGELYLAGTDGYRLAERRLMKSRSTIEAIVPSSSLQEALRTINDGVSEIEVLFDN
                     TQVRFRVGDAEITSRLIDGNYPDYRQLIPKESETNITVSTSEFTRIVKIAGLFSRDSG
                     GSVTLMADKEAGQLRIHSIASELGENTSSADATITADG"
     gene            complement(1212..2597)
                     /gene="dnaA"
                     /locus_tag="VLF64_01905"
     CDS             complement(1212..2597)
                     /gene="dnaA"
                     /locus_tag="VLF64_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HSW90729.1"
                     /translation="MYNALWQSVLGEIELSVPHGQFEAWFKNTTLLDYTDDAVTIGVA
                     NIFAKSQFEKRYDDKIREVLKHNGVAAPTVQYQVHTVKKSIVNRETTGDTDAMSARIE
                     SLVAPMPSVSVPASSSNQTISANTLNPRYSLDNFIVGSSNDLAYAACQAVAVNPGTKY
                     NPLFLYGGVGLGKTHLMQAVGNAITRQDPSAKVLYINTETFVKEYVEMIRFKKKSISD
                     KYRSVDVLIVDDMQFIAGKEKVQEEFFHTFNALHQANKQIIISSDKPPRSIPTLTERL
                     RSRFEWGMAIDIQMPDFETRCAIVETKAGLSGVNLEHDTVEYLAKNIKTNVRELEGAL
                     NQLLAYAEMRGVAPDVTTAEGLLGNVRQSRPQHVTSKQIIDKTARHFQIDAKEICSSR
                     RDKYIVVPRQIAMYLLRSELHMSFPRIAQELGRKDHTTAIHSVEKIEKSIKLDFMIRE
                     QVAEIREKLYA"
     gene            2859..2996
                     /gene="rpmH"
                     /locus_tag="VLF64_01910"
     CDS             2859..2996
                     /gene="rpmH"
                     /locus_tag="VLF64_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HSW90730.1"
                     /translation="MPKRTYQPRTRHRAKTHGFRARVASKAGRLVLKRRRAKGRAKLA
                     I"
     gene            3052..3405
                     /gene="rnpA"
                     /locus_tag="VLF64_01915"
     CDS             3052..3405
                     /gene="rnpA"
                     /locus_tag="VLF64_01915"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HSW90731.1"
                     /translation="MINATLRFHGHGSLRYLYKNGSAVRSHLFTLKYTVNPHRKKSRF
                     AVVVSKKVHKSAVGRNRIRRRVYEILRQEIPHLTTICDVAVIVSSGEVISIEHQELVA
                     VLQDLLVQAELLKKK"
     gene            3478..4404
                     /locus_tag="VLF64_01920"
     CDS             3478..4404
                     /locus_tag="VLF64_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HSW90732.1"
                     /translation="MFETIIVKPIFNALMLLYSIIPGGDFGVAIIIFTVIIGFVQYPL
                     VKQQLHQTKQMRKMQPELAKIKSAANGNKQLEATMQMELYKRYGINPFQSILVLIVQL
                     PIFIGLYQVIQVVTLHRNEVAKLAYEPVQHIPAIHKIIENPDNFNHTMLGFIDLTKTA
                     FSHGGVDITLVVLALLSAVTQYVISKQLLPTSDKPKKRFRDIMKEAASGKQSDASEMS
                     NAMMGNMSKFMPIMMFFIMINLPGALALYYTTSNLVAAAQQHYLLNKDTEEMDELADE
                     VIEQPKAKKGNNKGAQRAKKASEARITRIKAK"
     gene            4414..4875
                     /locus_tag="VLF64_01925"
     CDS             4414..4875
                     /locus_tag="VLF64_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HSW90733.1"
                     /translation="MDTAQSIEFARDFLKDIISFFGENIEVTATQEEDIIELYVETSD
                     LNSILIGRNAETLRSLQYIVSTALRNKNAELIRVNVDIADYKKQHEEKIAEKARGWIE
                     QVRATGDSYIAHINAADRRIVHQVATEYSDIRTYSEGEGRDRKIVIAQASS"
     gene            4919..5500
                     /locus_tag="VLF64_01930"
     CDS             4919..5500
                     /locus_tag="VLF64_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HSW90734.1"
                     /translation="MSGLVIAVIVIVVLAIIAAIIAGMYNGLVRLNVKAQEAWSGITV
                     QLKRRADLIPNLVESVKGYAAHESGVFEKVTEARSAIMNATQVGPAAAAQAEGELTGA
                     LKSLFAVAENYPDLKASQNFMDLQAQIADTEDKVAASRRFYNSTVTLFNTKRKVFPTN
                     IFANMLGFTQDKEFFDVPEGERQAVEQAPEVKF"
     gene            5547..6872
                     /locus_tag="VLF64_01935"
     CDS             5547..6872
                     /locus_tag="VLF64_01935"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HSW90735.1"
                     /translation="MPLELFARAGGGGSGGGGSGDGSGGIFIFLGYIPMHAVGAVLQR
                     VSAKNKALKIAINIIGWIIAVIYAVIWMVIWRGFGFFVGMAALAGMAGGLYNLFAKLK
                     QSKQTQAELAAASANDSVWSEEKLVNHARQVFMQYQKDWSNLNIQAMQGYLTPYYYNH
                     AALLAYTLKAMGRRDNMEDVKIQNAVILGVQNDTDDSRDAFTIGITAQANDQLINTVT
                     DQEIFTDRKPFTEYWTFQRSGNTWLLGGISQATADPYALNGQLRTFAANNGYYYSLDM
                     GWLFIPQHGQLFGEAKFGTSDINNHIVGMYNNQMLVQLYSYVKNPQSSTKPYVIAQVN
                     VPKQYGNIIVRRKKTFRMGIRGLEKVETEWTQFNDKYEVFASSAEQATSFELLNPTYM
                     EQLEALPFEVSIEVVDNVIYLYTDELTATLEIYEAMLDLVNKAFKELRY"
     gene            complement(6869..7957)
                     /locus_tag="VLF64_01940"
     CDS             complement(6869..7957)
                     /locus_tag="VLF64_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HSW90736.1"
                     /translation="MHIAIDARIINSGTGMYVAKLLEYLQQIDTTNQYSVLLRGKDID
                     YWKPTAPNFSVKVADFDNYSLAEQIGFKKFLDKLAPDLVHFCMPQQPVLYRGRHVTTM
                     HDLTLLNTYNSDKNWLVFHIKQLVGRWVFKRIARTNEHIIAISENTKREYEQFSHIPN
                     NKISVVYEAGEVYKGELEPYEVPYDEFIMYVGQQPDYKNLRRLAAAHQELLADNPKLG
                     LVFVGRMNEDTMRNKAFYEKHGYKNIHFTGFIPDSQRDWLLTKTRAYVFPSLMEGFGL
                     PPLEAMAYGTPVVSSNASCLPEILGDAAEYFNPTDIHSMAETIDRVIHNDKLRASMIE
                     RGTKQVGKYSWRRMAEQTHEIYMNALDS"
     gene            8006..8557
                     /locus_tag="VLF64_01945"
     CDS             8006..8557
                     /locus_tag="VLF64_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90737.1"
                     /translation="MTEFANHIRTLIEGHGDDKIEMIADIIGFATKAGYTVADVDDTK
                     PWGGFVRFDYKDGDRFIAEFFPDVNPVEARLGNPNAELSPKILLVAPEQRLSWQVHAR
                     RAERWKFLNDGGYHKSTDPDNPGEIIEAHTGDEVQFEAGECHRLVGANNHYTLVAEIW
                     QHIDKNNPSDEADITRLQDDYKR"
     gene            complement(8554..8982)
                     /locus_tag="VLF64_01950"
     CDS             complement(8554..8982)
                     /locus_tag="VLF64_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HSW90738.1"
                     /translation="MSLTEKLKNSHQHQVVRYGAVGVINTVIDFGLLFLLKSAGLPVE
                     LANICSTGTAFIFSFFANKKYTFKTTDTNVVREMVLFVIVTLFGLWVLQTIVINLTMP
                     MARQITSDQNIALLIAKLIATVVSLVWNYLLYNKLVFKKD"
     gene            complement(8954..9904)
                     /locus_tag="VLF64_01955"
     CDS             complement(8954..9904)
                     /locus_tag="VLF64_01955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSW90739.1"
                     /translation="MKKLISLLIPAYNEEEVLSLLFDRLETLADTNPEYDFEFFFVND
                     GSRDNTYELIRAQADKDGRVAYINFSRNFGKETAMLAGFDNVRGDAMVIIDADLQDPP
                     ELIPQMIKYWEEGYDDVFAKRRSRAGESFAKKKTSQWYYKILQSSTRIPIQVDTGDFR
                     LLDRRCIEALKQFRESERYTKGMYSWIGYKKKEILYDRDPRAAGQTKWNYPKLINLAV
                     DGITSFTTAPLRISSVIGSIVSVVAFVYILYLLIRPIFGVPTGGGYSSTMAVILFLGG
                     VQLLSIGIIGEYIGRIFNETKHRPLYFIEELHESHRKAEK"
     gene            complement(9914..>10129)
                     /locus_tag="VLF64_01960"
     CDS             complement(9914..>10129)
                     /locus_tag="VLF64_01960"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641667.1"
                     /note="Catalyzes the only substrate-level phosphorylation
                     in the TCA cycle; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HSW90740.1"
                     /translation="IAAADYIKDHVTKPVYAYIAGHSAPVGVQMGHAGAILGNNNQES
                     APAKTEYLEKSGAITADSITELVNRVQ"
BASE COUNT         2856 a         2304 c         2254 g         2715 t
ORIGIN      
        1 tggccatccg ccgtaattgt cgcatcagca cttgaagtgt tctcgccaag ctcgctcgca
       61 atcgaatgga tacggagttg tcctgcttct ttatcggcca tcagtgtgac gctaccgcca
      121 gagtcacgtg agaaaagccc cgcaatcttc acgatacgtg taaattcact tgttgatacc
      181 gtaatatttg tctctgattc tttaggaatg agttgtcggt aatcagggta attaccgtca
      241 atcaaacgac tggtaatctc agcgtcacca accctaaagc gcacctgggt attatcaaac
      301 aacacttcaa tctcactcac accatcgtta atcgtcctaa gagcctcctg aaggctcgag
      361 gaaggcacga tagcttcaat agtgctccgt gacttcatta agcgtcgttc agccaaacga
      421 tagccatctg tgccagccag gtacagctcg ccatcgatag agtgccagta cacgcccgtc
      481 aagactgggc gagaagtgtc gctgctcgtt gtaataatcg tttgattaac tgcttgtttg
      541 aaatcttcag tggtaatcgt gtacgcaacc gcctcttttt cctcaatcga cggcagttca
      601 gggaattctg atgcatcgac tccagtgaga ttcgacgaga aattaccaga agcaattgtc
      661 attgaggtgt ttttgctcat aatatccacc gtgcctttag gaaggctcgc aatgaactca
      721 ctgacgaggc gtgcaggcag agtaagctcg ccttgctttg tcacttttgc gcctacatag
      781 taggtagccg caatctctaa gtttgtcgct gctactaata ggcgattacc ttctgtgcgc
      841 agcagtacat tattcaagat cggcagtccg gttcgactac tcgctacacg accgacaatg
      901 ctaagtgccc gtgccagatt ttcttgtgtg accgagagtt ccattattat taaatatcct
      961 ttctaatgaa ttgatagtaa tgatagtagg tgctgtggat agtgggtaaa acaatactca
     1021 tagcaggggt aatactgcta tttctgtatg tggatagtgt aggcagggta actgtgtata
     1081 cagcatgggt gaaatagtta tttatccacg tacattggac gactgcttgg gtttgggcta
     1141 aatcagtgta caaacgaaca cattttttac tagcagcttt acgcaagtaa ttcacaccgt
     1201 ttccacaggc cttaagcata gagtttctcc cgaatctcag cgacttgctc gcgaatcata
     1261 aaatcaagct tgatagactt ctcaatcttc tcaactgaat gaatggcagt tgtgtgatct
     1321 ttgcggccga gctcctgggc gatccttgga aaactcatat gaagctcgct gcgcagtaaa
     1381 tacatcgcaa tctggcgtgg caccacaatg tatttgtcac ggcgactgct gcaaatctct
     1441 ttcgcatcaa tctggaaatg acgtgctgtc ttatcgatga tctgttttga agtaacatgc
     1501 tgcgggcgtg attgacgcac attaccaagg aggccctctg cagtagtaac atctggtgca
     1561 acaccgcgca tttctgcgta ggcgagcaac tggttgagtg cgccttctag ctcgcgaaca
     1621 ttggttttaa tattctttgc caagtactca actgtgtcat gctcaagatt cacaccagat
     1681 agaccagctt ttgtttccac aattgcacaa cgtgtctcaa aatctggcat ctgaatatcg
     1741 attgccatac cccactcgaa ccgactgcga agacgctcgg taagagtagg gatactgcgc
     1801 ggcggtttgt cgctactaat aataatttgt ttgttcgcct ggtggagcgc gttgaaagta
     1861 tggaagaatt cttcctgcac cttttctttg ccggcaatga actgcatgtc atcaacaatc
     1921 aacacatcaa cagaacggta tttatcagaa atactcttct ttttgaagcg aatcatttcg
     1981 acatattctt taacaaaggt ttccgtatta atatagagca cttttgctga aggatcttgc
     2041 cgggtaattg cgttacctac tgcctgcata agatgggttt taccaaggcc tacgccacca
     2101 tagagaaaga gtggattgta cttagtgcct ggattaacgg caactgcctg gcaggctgca
     2161 taggcaaggt cattacttga tccaacgatg aagttatcga gcgaatagcg ggggtttaac
     2221 gtattagcac tgatggtctg atttgatgat gatgccggta ctgacacaga aggcattggt
     2281 gcaactaaac tctcaatgcg agccgacatc gcatcggtat cgccagttgt ctcgcgatta
     2341 acaatactct tctttacggt atgtacttgg tactgcacgg ttggcgcggc aacgccatta
     2401 tgcttcagca cctcgcgaat tttatcgtcg tatcgcttct caaattgtga cttagcaaag
     2461 atattggcca caccaatcgt tacagcatcg tcggtataat caagaagtgt cgtgtttttg
     2521 aaccacgctt caaactgccc atgaggaacg gatagttcta tctccccaag tacactttgc
     2581 cacagtgcgt tatacattca ctccccctgt tcgtttatct gttcctatga taccgataat
     2641 caaagttttc cacaactaaa caagtgttgt ggattttcac atcacactaa caggcttaac
     2701 tagtggtgca tttatccaca aatggcgacc acccctgtta atctgtggat aactgtcgcc
     2761 tgaatgtgga taaaattata tgaccaggat atattttaga caaaaattca aggagatatt
     2821 gataaaagat agaacaaagg ttataatacg ttcttgatat gccaaagcga acttaccaac
     2881 cacgcacacg tcaccgtgca aagactcacg ggttccgcgc acgtgtagcg tcaaaagctg
     2941 gccgacttgt tcttaagcgc cgccgcgcta aaggtcgcgc taaactcgct atctagcgaa
     3001 taaaatcacc gctgtacatt cagcggtgat tttattatgt atacttatac tatgattaat
     3061 gcaacgttac gtttccacgg acacggcagc ctgcgctacc tttacaaaaa tggcagcgcc
     3121 gtacgttcac atttgtttac gctcaaatac accgtcaatc cacaccgtaa gaaatcacgc
     3181 ttcgcagttg tagtgagtaa aaaggtacat aagtcggcag ttggccgtaa tcgcattcgt
     3241 cgtcgcgttt acgaaatact tcgtcaggaa attccgcacc ttactactat ctgcgatgtg
     3301 gcggtgattg tctcaagtgg tgaagttatt tctattgagc atcaagaact cgttgccgta
     3361 cttcaagatc tactcgtcca ggctgaactc ctcaaaaaaa agtagccttc ggcctctggt
     3421 aaaaccccga gggtatcgtg ctataattat aaggagtaag atttatctgg agtattcatg
     3481 tttgaaacaa ttattgtaaa gcctattttc aacgcattga tgctacttta cagcatcatc
     3541 ccaggcggcg actttggtgt tgcgatcatc atcttcacag tgataattgg cttcgttcag
     3601 tatccgcttg ttaaacagca gttgcatcaa accaagcaga tgcgtaaaat gcagcccgag
     3661 ctcgcgaaaa tcaaaagcgc agcaaatggt aataagcagc tcgaagctac catgcagatg
     3721 gagctctata agcgctacgg tatcaaccca ttccaatcca tcctggtgtt aattgttcag
     3781 ctgccaatat tcattgggct ctatcaggtg attcaggtgg taacgcttca ccgtaacgaa
     3841 gttgcgaagc ttgcatacga gccagtgcag catattcctg caattcataa gattatcgaa
     3901 aacccagata actttaacca cacaatgctt gggtttattg atcttactaa gacggcattc
     3961 agccatggtg gcgtagatat cacactagtt gtgttggcgc ttctttctgc tgtgactcaa
     4021 tatgtcatca gcaagcaact tctgccaaca agcgacaaac cgaagaagcg ttttcgcgac
     4081 atcatgaaag aggcagcttc tggcaagcag tctgatgcga gtgaaatgag caacgcaatg
     4141 atgggcaata tgtcgaaatt tatgccaatc atgatgttct tcattatgat taacctgcca
     4201 ggtgcactcg cactttatta cacgacgtct aaccttgtag cggcagctca gcagcactat
     4261 cttctcaata aagacaccga agagatggat gagcttgctg acgaggttat cgaacaaccg
     4321 aaagccaaga agggtaacaa taaaggtgct cagcgtgcga aaaaggcaag cgaagcccgc
     4381 atcacccgaa ttaaggcgaa ataggaggta catatggaca cagcacaatc aattgaattc
     4441 gcacgagact tcctcaaaga catcatttca ttttttggtg aaaacattga ggtaactgca
     4501 acacaagaag aggacatcat cgagctctat gttgaaacga gtgacctcaa tagtatcctt
     4561 attggccgta atgccgaaac acttcgcagc ctacaatata tcgtaagtac cgcacttcgc
     4621 aacaaaaacg ccgagctcat tcgtgtaaat gtcgatattg ccgactacaa aaaacagcac
     4681 gaggagaaga ttgccgaaaa agcacgaggc tggattgagc aagtacgtgc gacgggtgat
     4741 tcatacattg cccatattaa cgccgcagat cgccgtattg tgcaccaggt agcgactgag
     4801 tattcagata ttcgtaccta ttcagaaggt gaaggccgcg atcgcaagat tgttattgct
     4861 caggcaagct cgtagtgtat tagaataagt agtaagcata agaaaatagg agaataaaat
     4921 gtcaggatta gttatcgcag tcattgttat cgtcgttttg gccattattg ctgcaattat
     4981 tgcaggtatg tacaacggcc tcgttcgctt aaatgtaaaa gcccaagaag cgtggagtgg
     5041 tatcaccgta cagcttaagc gacgtgctga cttaattcca aaccttgttg aatctgttaa
     5101 ggggtatgcg gcacatgaaa gcggagtctt cgagaaagta actgaagcac gttcagcaat
     5161 catgaatgca acccaagttg gccctgctgc agctgcacaa gccgaaggcg aactcacagg
     5221 tgcgctcaag agcctcttcg cagttgccga gaactatcca gatcttaaag cttcacagaa
     5281 ctttatggac ttgcaggcgc agatcgccga caccgaagat aaggttgctg catcgcgccg
     5341 cttctacaac agcacagtga cgctttttaa cacgaagcgc aaagtattcc ctacaaacat
     5401 ctttgcaaac atgcttggct ttactcagga taaagaattc ttcgacgttc ccgagggtga
     5461 gcgccaggcg gtcgagcagg ctcctgaagt taaattctag ccgctggtag gttccagtgc
     5521 taacgtccct attcacaatc gctacattgc cgctcgagct ttttgctcga gctggtggcg
     5581 gtggttcagg cggaggtgga tccggtgatg gcagcggcgg aatctttatt tttcttggct
     5641 atattccaat gcatgcagtg ggtgcagtac ttcaacgtgt tagtgcaaaa aacaaagcac
     5701 tgaagattgc aataaatatt attggctgga tcatcgcagt catctacgcg gtaatctgga
     5761 tggtaatctg gcgtggattt ggattttttg ttggtatggc tgcgcttgct ggtatggcgg
     5821 gtggccttta caatctcttt gcaaaattaa agcaaagtaa acaaacacag gctgaattag
     5881 cagctgcgag tgctaatgat agtgtttggt ctgaagagaa gctcgtcaat cacgccaggc
     5941 aagtatttat gcagtatcaa aaggattggt ctaatcttaa tattcaagcg atgcaaggct
     6001 atttaacgcc gtattactac aatcatgcag cgctacttgc atatacactt aaagcaatgg
     6061 gtcgtcgcga caatatggag gatgtaaaaa tacaaaacgc cgtgatcctc ggggttcaaa
     6121 atgacaccga tgatagccgc gatgctttca caatcggcat caccgctcaa gcaaacgacc
     6181 aattgataaa cactgtcact gatcaagaaa tctttactga ccgcaagccg tttaccgagt
     6241 actggacatt ccagcgtagc ggtaatacat ggctgctcgg cggcatttct caagcgacag
     6301 ccgatccgta cgcgctcaac ggccagctcc gtacatttgc agcaaataac ggctattatt
     6361 atagtctcga tatgggttgg ttgtttatac cacagcatgg tcaattattt ggtgaagcaa
     6421 aatttggcac atcggatatt aacaaccata tcgttggcat gtacaacaat cagatgctcg
     6481 tacagctcta tagctatgtc aaaaacccac agtcgagcac gaagccctac gtgattgcgc
     6541 aggtaaacgt tccaaaacaa tacggcaata ttatcgtacg ccgcaagaaa acattccgta
     6601 tgggtatccg tggccttgaa aaagtagaaa ctgaatggac acagtttaac gataaatatg
     6661 aggtgtttgc atcgagtgcc gagcaagcaa caagtttcga attgctcaac ccaacctata
     6721 tggaacaact cgaggcactt ccatttgagg tgagtattga agtcgtagat aatgtaatct
     6781 acctctatac cgatgagcta acggccacgc tcgaaattta tgaagcaatg cttgatctcg
     6841 taaacaaggc ctttaaagaa ttgcgctact agctatcaag tgcgttcata tagatttcgt
     6901 gagtttgctc cgccatgcgg cgccacgaat atttgccaac ttgctttgta ccgcgttcaa
     6961 tcatactggc gcgtagctta tcgttatgaa taacgcgatc gatagtctca gccatactgt
     7021 gaatatcggt tggattaaaa tactcagcag catcaccaag gatctcggga aggcaagatg
     7081 cattactgct cacgactggt gtaccatacg ccattgcttc aagtggtggt aagccaaaac
     7141 cttccataag tgaagggaat acatatgctc tggttttagt aagtagccag tcacgttggc
     7201 tgtcggggat aaatccggta aaatggatat ttttataacc gtgtttctca taaaacgctt
     7261 tgttgcgcat cgtgtcttca ttcatgcgac ccacaaatac gaggccaagc tttggattat
     7321 cagcaagaag ctcttgatgc gctgccgcaa gtctacgcaa attcttgtaa tcgggctgct
     7381 ggccaacata cataataaac tcatcatatg gcacttcgta gggttcaagc tcacccttat
     7441 agacttcacc agcctcgtaa acgacagaaa ttttattgtt gggtatatgg ctaaactgct
     7501 catattcacg cttcgtattc tcgctaatag caatgatatg ctcatttgtg cgagcaatac
     7561 gcttaaatac ccaccgtcca accaactgtt tgatatgaaa tacgagccag tttttatcac
     7621 tattatatgt attgaggagg gtaagatcat gcatcgtcgt gacatgccta cctctataaa
     7681 gcactggctg ttgtggcata caaaaatgca ctaaatctgg tgcaagttta tcaagaaact
     7741 ttttaaaacc gatctgttcc gcaagtgagt aattgtcaaa atcagccact ttaacggaaa
     7801 agttaggtgc cgtaggcttc caataatcaa tatccttgcc acgcagtagt acggaatatt
     7861 ggtttgtcgt gtctatttgt tgtaaatatt ccaacagctt tgccacatac ataccagtac
     7921 cagagttgat aatacgggcg tctattgcaa tgtgcatata ctcttaatat acagtattat
     7981 agagtaacca caaaggagga taacgttgac tgaatttgca aaccacatcc gtacgcttat
     8041 tgaaggacac ggagacgaca agatagagat gatcgcagat atcataggtt tcgccacaaa
     8101 ggcaggctac acagtagcag acgttgatga tacgaaacca tggggcggat ttgttcgatt
     8161 tgactacaaa gatggcgacc ggtttattgc tgaattcttc cccgatgtca acccggtaga
     8221 agctcgttta ggtaatccta atgccgagct aagccctaaa atcttactcg tagctcccga
     8281 gcaacgctta agctggcagg tacacgctcg ccgcgccgaa cgctggaagt ttctgaatga
     8341 tggcggttac cacaagagca ccgaccctga taacccgggt gaaattatcg aggcccatac
     8401 aggcgacgaa gtgcagttcg aggctggcga atgccatcgt ttagttggcg caaacaatca
     8461 ctatacactt gttgcggaaa tctggcagca catagacaag aataatccat ctgatgaagc
     8521 ggacatcaca cgtctccaag acgattacaa gcgttagtct tttttgaaca ccagcttgtt
     8581 atatagtaga tagttccaaa cgagcgatac tactgtcgca ataagcttag caatcagtag
     8641 ggcgatgttc tgatcgcttg tgatttgcct tgccatcggc attgttaggt taataacaat
     8701 tgtttgcagt acccataatc caaatagtgt gacaatgacg aaaagcacca tttcacgtac
     8761 aacatttgta tcagttgttt tgaacgtata tttcttatta gcaaagaagc taaagataaa
     8821 tgctgtgcct gttgaacaga tattagcaag ttcaacgggt aggccagcag acttcaaaag
     8881 aaataacaaa ccgaagtcga taaccgtatt aataaccccg acagcaccat atcgtacaac
     8941 ttggtgttga tggctatttt tcagcttttc ggtgagactc atgaagctcc tcgataaaat
     9001 aaagtgggcg gtgctttgtt tcattaaaga tacggccaat atactcacca ataataccga
     9061 tactaagtaa ctgaacacca ccaagaaaca agataacggc catagtcgag gaatagccac
     9121 caccggtcgg tacaccaaag atagggcgaa tcagtagata gagaatatat acgaaggcaa
     9181 caacagacac gatactgcca ataacactcg aaatacgcag cggcgcagta gtgaagcttg
     9241 taataccatc aacggcgagg ttaatgagtt taggatagtt ccatttcgtt tgtcctgcag
     9301 cgcgcgggtc acggtcgtag aggatttctt tcttcttgta ccctatccag ctgtacatac
     9361 ctttggtgta gcgctcactc tcacggaatt gcttgagcgc ctcaatacag cggcgatcga
     9421 gtaaacgaaa gtcaccagta tcaacctgaa tggggatgcg ggttgagctt tgtagtattt
     9481 tgtagtacca ctgactcgtt ttcttctttg caaaactttc accagcacgg ctacgacgct
     9541 ttgcaaacac atcgtcataa ccctcttccc aatatttgat catctgcggg ataagctctg
     9601 gcggatcttg tagatcggca tcaataatca ccattgcgtc gccgcgcacg ttatcaaacc
     9661 cagcaagcat cgcagtttct ttgccaaagt tgcgactaaa gttaatgtag gcaactctgc
     9721 cgtctttatc tgcctgggcg cgaataagtt cataggtatt atcgcgactt ccatcgttta
     9781 caaagaagaa ttcaaaatcg tattctgggt tggtatctgc gagcgtttca agacgatcaa
     9841 agagaagact aagtacttct tcttcgttat aggctgggat aagcagggaa ataagctttt
     9901 tcatagttat acactattgt acacggttta cgagctctgt aatactgtct gcggtaatcg
     9961 ccccagactt ttctaggtat tcagtcttag cgggcgcgct ttcttggtta ttattgccca
    10021 ggattgctcc agcatgcccc atctgcacgc caactggcgc actgtggccg gcaatgtacg
    10081 cgtacacagg cttggtcaca tggtctttta tgtaatctgc ggccgcaat
//
LOCUS       DATIIX010000065         3492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_155552, whole genome shotgun sequence.
ACCESSION   DATIIX010000065 DATIIX010000000
VERSION     DATIIX010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155552"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VLF64_01965"
     rRNA            <1..411
                     /locus_tag="VLF64_01965"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            601..710
                     /gene="rrf"
                     /locus_tag="VLF64_01970"
     rRNA            601..710
                     /gene="rrf"
                     /locus_tag="VLF64_01970"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            1341..2819
                     /locus_tag="VLF64_01975"
     CDS             1341..2819
                     /locus_tag="VLF64_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HSW90741.1"
                     /translation="MRTLELKLDTPDVLYTYHVQVGKEIAQCRAVLGKALLSCGLAPL
                     ALLQGRQYQIEVLRSFRSEAPSAVLKAMITTLTATTIVNGSVGGGDTVYAKPMEFTFT
                     ADKPLKSAIAILTQEGAASAMGTTTVVKGKVITIKLAGELLREKAYILKITNLEAADG
                     SSLVEPYIVAFHTSGGPKVTNVSVGSSGVATNAHVVVTFDQQLSDSQDISKLVVISGV
                     NAQITRSGNQIIYTLSAGLCVPFTLSVAKGLLSSYDIASVADWSYTSRTICHTTLTYG
                     YSVHGRALVAYAFGTGGPVTMYVGAIHGNEPSSSGLMRAWTDDLEANPSLYSGKRVVV
                     VPTINPDGVAANTRTNAHGVNLNRNFPTDGWVKDINDTDGYHAGGGGSEPLSEPEARA
                     LASLTTSWQPRLLLSFHAVGSLVMGDPGGYSVGYAAKYASMVGYSDTTYSLNGGFDYN
                     ITGAYEDWTASKQGIPSMVIELGSYGYYSFSHHQAALRAMLN"
     gene            3231..3461
                     /locus_tag="VLF64_01980"
     CDS             3231..3461
                     /locus_tag="VLF64_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90742.1"
                     /translation="MAGTKAGGLKAAQKNLAKDPNFYAKIGAKGGSNGHTGGFAANPD
                     LARVAGAKGGRISRRTKKADDSQAVQATPVAE"
BASE COUNT          932 a          709 c          890 g          961 t
ORIGIN      
        1 gacggcgctg tttggcacct cgatgtcggc tcatcacatc ctggaggggg agcaccttcc
       61 aagggttcgg ctgttcgccg attaaagtgg tacgcgagct gggttcagaa cgtcgtgaga
      121 cagttcggtt tatatccggt atggacgtca ggaattttga gaggatctac ccctagtacg
      181 agaggaccgg ggcggacgca cctctggtgt acggattgtg gccccctgct gcattgtccg
      241 gtagctatgt gcggaataga taagcgctga aagcgtatta agcgcgaaac taactccaag
      301 attagatttc tttatgagga cactgaaaga atatcagttt gataggtgca aggtggaagt
      361 gcggtaacgt atggagccaa agcatactaa tagtcccatt gcctttttat gttctcttta
      421 ccactgttac tgcttgcaat aacgacggta aagggataga cttaactagt aattgatgtt
      481 cataaaagaa tatcagacgt cagtctttga catcaccaag cccgtgcatc acgttacgga
      541 catcgaaata aggcttttcc caggttgcct gtatggaacc tctgaggagc aaagccttat
      601 ttcggtgccc atggcgatga ggaaacacct gttctcattc cgaacacaga agttaagctc
      661 atcagcggcg attatactgc cacaagtggg aaactagcac ggcgccgaat tatacaataa
      721 cccctgggaa taccaggggt tttgtcttgt ttgaaattgt ttaaccaaaa ttctatttct
      781 tagcagatat gcgcgtcgta tacttattac tatacaagtg ggtataaata aaaagaggtg
      841 gtaaaatgaa aatttctgtt gaatatgtcg acgctgaact agctgttgct gttcgtcggt
      901 agtattagag gggtagattg atatataaat acatcaatct tattaaaact tgggtgcgat
      961 tacggaatct gcgcacctgg gtcattgctc ttgtagctat aagcctcctc gtatatattg
     1021 tactttggat tattcccagg ccactcgcat tgtcgtatgc atctgagcgt acatgtattg
     1081 tgtggcctac gtttttgcca ggagtccaga aaacggtgga cggtacgaag ttttcggtgt
     1141 cgactgaggg tggcctgcag atagggagca tccgtttgct atcaacgcac acctgcatag
     1201 agccagtaaa aaacttcaat gctggacgta ctatagtagc gttatctccg tttgggagct
     1261 ggctatttca gcaacgtata atggtggtaa cgagtaacgt tcctagtgtt agtttgaata
     1321 ccgataaccc cataccggct atgcgtactc ttgagctaaa gcttgatacc cccgatgtgt
     1381 tatatacgta tcatgttcag gtgggtaaag agatagcaca gtgtcgggcg gttttaggta
     1441 aggcgttatt atcatgtggc ctagccccgc ttgcgctcct acaaggtagg cagtatcaga
     1501 ttgaggtgtt acgatcgttt aggtctgagg ctccatcagc tgttctcaag gcgatgatta
     1561 cgacccttac cgcaactacg atagtaaatg gatctgtagg aggcggtgac acggtctatg
     1621 ctaagcccat ggaatttacg ttcactgccg ataagcctct gaagagcgcg attgcaattc
     1681 ttacacagga gggtgcagcc agtgctatgg gtacaactac agtcgtaaaa ggtaaagtca
     1741 tcaccattaa acttgctggt gagttactac gagaaaaagc ttacatatta aagattacga
     1801 atcttgaggc cgctgatgga agttcgctgg ttgagccata tatcgtggca tttcatactt
     1861 caggtggccc aaaagtcact aacgtgtctg tcggtagttc gggtgttgcg acaaatgcac
     1921 atgttgtcgt tacatttgac cagcaattgt cagactctca ggatataagt aagcttgtgg
     1981 tgatatcagg agttaacgcg cagataacaa gaagtggcaa tcaaatcata tatacattga
     2041 gtgcgggttt atgtgtacca tttactctta gtgttgctaa gggcctacta agtagctatg
     2101 atattgcctc tgtcgcggat tggtcatata catcacgcac tatttgtcat acaaccttga
     2161 cgtatggtta ctcagtgcat ggtcgagcct tggtcgcata tgcatttggt acaggaggtc
     2221 cggttactat gtatgttggt gcaattcatg gcaatgagcc aagttcaagt ggacttatgc
     2281 gagcctggac ggatgattta gaagcaaatc cgagtttgta tagtggcaag agggtagttg
     2341 ttgttccgac tattaaccct gatggagtag ctgcaaatac tcggacgaat gctcatgggg
     2401 ttaatttgaa tcgcaacttc ccaacagatg ggtgggtgaa ggatattaat gatacggatg
     2461 gatatcatgc agggggaggg ggctctgagc cactttcgga gcccgaggcg cgggcacttg
     2521 caagtcttac tactagttgg cagccacgat tgctcttatc gtttcatgca gtggggagtt
     2581 tggtgatggg agacccaggc ggatactctg taggctatgc agctaaatac gcatctatgg
     2641 ttggctatag cgatacaacg tatagcctaa acggagggtt tgattataat attaccggcg
     2701 catatgaaga ttggacggcc tcaaaacaag gtattccaag tatggtaatt gagctcggca
     2761 gttatgggta ctatagcttt tcacaccatc aagcagcgct gcgtgccatg ctgaattaaa
     2821 actgtccacc tctggtggct agaagagatt gactcggctg agtaacccaa agctgcatag
     2881 aatagaaaag catagcattc ctaataaaat aacccgtctc gcaggcgggt tattttttgc
     2941 tttctcacgt ctcgcaggcg tgagaacctt tttgtggtga atctcgtagt ttttcgaggt
     3001 tcaaacaggt gcatatctat aatatcgcac agcatgcata agagcaatac tttttatacc
     3061 ataattactt tgcttgtaac cattgacact ttgaattgtt tgtgctaata tagacttata
     3121 cacatctcag atgtgaggta taagcgaggg tttcgaacca tctaccgcag gtaggagggc
     3181 ggaacccttt taatttgctc ggtatattcc cgggcgagga gaacctacaa atggcaggta
     3241 caaaagctgg cggccttaag gctgcacaaa agaacttagc taaggatcct aatttttacg
     3301 ctaaaattgg cgcgaagggc ggtagtaatg gtcacacagg cggatttgct gctaacccag
     3361 atcttgcacg tgttgcaggt gctaagggtg gccgtatctc acgtcgcact aagaaggctg
     3421 acgacagtca ggctgtacaa gctactccag ttgcagagta gtcaaaccgt ataaaagtct
     3481 gaaaaacatc tc
//
LOCUS       DATIIX010000066         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_15924, whole genome shotgun sequence.
ACCESSION   DATIIX010000066 DATIIX010000000
VERSION     DATIIX010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15924"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VLF64_01985"
     CDS             <1..700
                     /locus_tag="VLF64_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deoxycytidine triphosphate deaminase"
                     /protein_id="HSW90743.1"
                     /translation="EIKNAVAEGTIVSVPYNEAHVSEASLDFTLGYYFYKQEVREDNR
                     VYNPFDAGDVAQYFKGPLQAIPHKEWCDQYGYKPFDNIPMDHPIIVLRPGERILAHTH
                     EFMGIRAHGGAAEVRARSSWGRNGVAVCFDAGWVDPGYINRITLEIYNLNKYESVVLP
                     VGERMGQLVFHKTGKVDGDYSHGREGMSGKYQTTDDLDKLIANWRPSDMLPRNHKDKR
                     VKPLTIKGLSEGIL"
     gene            710..1396
                     /locus_tag="VLF64_01990"
     CDS             710..1396
                     /locus_tag="VLF64_01990"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90744.1"
                     /translation="MSKRGRFFAIEGGDGSGKGTQTSVLAEALRAAGRDVYQLSFPRY
                     GTHSAAIVEKYLRGEFGEANDVAAELASAAFALDRVAAKPELDEWLDNHTDGIALADR
                     YVLSNMAHQGTKIPDQDKRFAFYKDILDLEFGDLRLPRPDKNVILLVPPDIAQENVDK
                     KAARSYTTAKRDIHEKDSSHLTLANRNYREIAELFPEFAVAIEAYDPKAEQMRPIEDI
                     QTEIRRIFNL"
     gene            1435..>2697
                     /locus_tag="VLF64_01995"
     CDS             1435..>2697
                     /locus_tag="VLF64_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90745.1"
                     /translation="MEHRTDKRDAHKSEHVLSGVTLVDMPVSIHDTYQVFWSASGLSR
                     AEIAAHEHELGLTTMLLADFCEEYVSPSGYTAAMLSPILEAATSETTHAQAYEALRAY
                     ANNTSESEGEKRYVIGLASDRPIIADFWSSNVADQYTADPELQYLLQSNQTTNVSIDQ
                     WSKHARSIEPKYINELASSVNIESLIIKAAELLAQLDPSTATNDNQTLSRIHQAEALL
                     APLCEIIGFDALAMALHSHVHVLRALFTGNIAPLQRAQEIIAERGDKEQVDSDVQQLF
                     SVVFGENIHEQIIKHGAEHGIMMGEGFCTSECLRVNWRLKSLGSLTKKLNALPPERLP
                     LDILGVTVTTPDVPQIASRLKSILERSRNDGRITLTPTAERTAAIHIKGPNEYIEIIR
                     EGLGFESLAAMREVADVIEVDEGAHRVCK"
BASE COUNT          775 a          668 c          644 g          610 t
ORIGIN      
        1 tgaaattaaa aatgcggtag cagaaggaac aattgtcagc gtgccatata acgaggcgca
       61 tgttagcgaa gccagcctcg attttacgct gggctactac ttttataaac aagaggtacg
      121 tgaagacaat cgcgtgtata atccgttcga tgctggtgat gtcgcacagt attttaaagg
      181 tccactccaa gcgattccgc acaaggaatg gtgcgaccaa tatggttaca aaccatttga
      241 caacattcca atggaccacc cgatcatcgt gctacgtcct ggtgagcgca ttttagcgca
      301 cacacatgaa tttatgggta tccgtgcaca tggtggagct gccgaagtcc gcgcgcgtag
      361 cagctggggt cgcaacggcg tagcagtttg ttttgacgca ggctgggtag acccgggcta
      421 tatcaaccgc attacactcg aaatctacaa tctcaacaaa tacgaatctg ttgtgctgcc
      481 agtaggcgaa cgtatgggtc agctggtgtt tcacaaaacc ggcaaagtag atggcgacta
      541 tagccacggc cgggaaggca tgagtggtaa atatcagacc accgatgatc ttgataagct
      601 cattgccaac tggcgcccaa gcgatatgct accacgcaat cacaaagaca agcgcgtcaa
      661 gccgcttact atcaaaggcc tgtcagaagg gatattgtag acagccatta tgagtaaacg
      721 aggacgtttt tttgccattg agggtggcga tgggtcaggt aaaggtacac agacttcagt
      781 tcttgccgag gcattacgcg cagctggccg cgacgtgtat cagttaagct ttccgcgcta
      841 cggaacacac tctgctgcaa tcgttgaaaa atatctgcgc ggtgaattcg gcgaagcgaa
      901 cgatgtagcg gcagagctcg ccagcgccgc ctttgcgcta gatcgcgtcg ccgccaaacc
      961 agagttagat gagtggctcg ataaccatac cgatggcatc gcgctcgctg atcgctacgt
     1021 attatctaat atggcacacc aaggcactaa aattccagat caagacaaac gctttgcgtt
     1081 ctacaaagat atcctcgatc ttgaattcgg cgatctgcga ctaccacgac ctgataaaaa
     1141 tgtcatattg cttgtgccgc ctgacatcgc tcaggaaaat gtcgacaaaa aagcagcaag
     1201 aagctacacg acagccaaac gtgacatcca tgaaaaagac agcagccacc tgacgctcgc
     1261 caatcgcaac taccgcgaaa ttgccgagtt attccctgaa tttgccgtag ctattgaagc
     1321 atacgaccct aaggctgaac aaatgcgccc gatcgaggat atccagactg aaatacgccg
     1381 tatatttaac ctgtgatttg acttatttaa ccttgggttg tataatatga actaatggag
     1441 catcgcacag acaagaggga tgctcacaaa agtgagcatg ttctttcggg cgtgacatta
     1501 gtcgatatgc ccgtgtctat tcatgatacc taccaagtct tctggtcagc cagtggacta
     1561 agtcgggccg aaatagcagc acatgaacat gagctcggcc ttactacaat gctgctcgcc
     1621 gacttttgcg aagaatatgt ttcgccaagt ggttacaccg cagcgatgct ttctcctatc
     1681 cttgaggctg caactagcga aactactcat gcacaagctt acgaagcgct gcgtgcatat
     1741 gcaaacaaca caagcgaatc tgaaggtgaa aagcgatatg ttatcggcct tgcaagtgat
     1801 cgtcccatca ttgccgactt ttggagtagc aatgttgcag accaatatac agcagatcct
     1861 gagctgcaat acctgctaca aagcaaccag acaaccaatg tatcaatcga ccagtggagt
     1921 aaacacgcac gatcaatcga gcctaaatat atcaatgagt tagcatcatc ggtcaacata
     1981 gaatccctca tcatcaaagc agccgaacta cttgcacagc tcgacccgtc aaccgcaaca
     2041 aacgacaatc aaacactgag ccgtatacat caggcggagg cgctccttgc tccactctgt
     2101 gaaattattg gtttcgatgc gctcgcgatg gcacttcata gccatgtaca cgtgctacgt
     2161 gctctattca ccgggaatat tgccccgttg caacgagcac aagaaatcat tgccgagcgc
     2221 ggtgataagg aacaggtaga cagtgatgtg caacagcttt tcagcgtagt atttggcgaa
     2281 aacatccacg agcaaattat taagcacggc gccgaacacg gtattatgat gggtgaaggc
     2341 ttttgtacga gcgagtgcct acgcgtaaat tggcggttaa aatctctcgg ttcgctaact
     2401 aaaaaactta acgctctccc accagagagg ttaccacttg atattctcgg agtaacagtc
     2461 accacaccgg atgtgccgca aatcgcgtca cggctcaaga gtattctaga acgctcacgc
     2521 aacgatgggc ggatcacatt aacacctaca gcagaacgca ctgcagccat ccatatcaag
     2581 ggtccaaatg aatatatcga gataattcga gaaggacttg gatttgaaag cctcgctgct
     2641 atgcgcgaag ttgccgatgt tatcgaagtt gatgagggcg cacatcgagt gtgtaag
//
LOCUS       DATIIX010000067         6136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_184112, whole genome shotgun sequence.
ACCESSION   DATIIX010000067 DATIIX010000000
VERSION     DATIIX010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184112"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..1085
                     /gene="pilM"
                     /locus_tag="VLF64_02000"
     CDS             39..1085
                     /gene="pilM"
                     /locus_tag="VLF64_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HSW90746.1"
                     /translation="MNILKGLGDFFALDIGTNAIRVVQLKKAGAGYALEHYGYAPIDS
                     KLTGSDSEEARRKVGEVIMTAVGQSGIKSKNVVIGLPSSKTFTTIIDVPMVSEAELKA
                     TMKYQIDQYIPMSVDDAKVDWALLGNSLRAQNQQEVLLSSVARQYSEDRLELVEALGF
                     NVIAAEPDPIAMVRALSVTGATGAVLQLDMGENSTDIAIVYGDTPRLVRTIPTGVGSL
                     VKSTVTNLNVQEDQARQFILKFGLAPDRLEGQVLRALESVLDNFAAEVVKSIKFFETR
                     YPSIAVGGVIVTGFAATIPQFDQYLAGKVGKGVQLGNPWQGIQMAEADQRQPAAVAME
                     FSTVIGLAKRGVEQ"
     gene            1082..1813
                     /locus_tag="VLF64_02005"
     CDS             1082..1813
                     /locus_tag="VLF64_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90747.1"
                     /translation="MIQINLVPDVKQEMLHAQRMRNVAISLSIVIGLVAVGIVVALAL
                     ILGGQVAVQKIEEGQITSGYSKLSSVSDLDSALTIQNQLGTISSQNDKRTVDSRLLDV
                     LAAINPPAPNDVKLSKVTLDPTQNTVTLEGSAVGGYPATDVFRKTILNTKITAKQQGN
                     DNAAETHLTSNVTLQDTSYGEDATGAKVVRFTIIFTYPNGLFDNRMSDVKISTPSARV
                     DVTDSKTLIPDSMFSQAATDLKENK"
     gene            1813..2496
                     /locus_tag="VLF64_02010"
     CDS             1813..2496
                     /locus_tag="VLF64_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90748.1"
                     /translation="MASQMVSPKRRAIADSSRTMFIWVAGMSAIVGICVVVAIFLVQQ
                     IMFKVKVVGAMTETVNTLNYNNKVAKDLTSNVVVLQTNSALNSVKANGDENALQVPLD
                     ALPAERNSLALGASLQQVLLAGVDGLSIDSLSVDTVDSNANTNIADTSATADNTISLQ
                     MQISSSNVNTIKSTLTRLENSIRVIDIDSFILERSDTTYQATITAHAYYQPGIKLQLQ
                     SKSIMPGGK"
     gene            2496..2729
                     /locus_tag="VLF64_02015"
     CDS             2496..2729
                     /locus_tag="VLF64_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90749.1"
                     /translation="MKKSDIAMVALIASVSVMISFVIANQISFLKPPQKGEDVKTIEK
                     INPDVTAPDTKVFRADAINPTVQTVIGGGQSAQ"
     gene            2751..4502
                     /locus_tag="VLF64_02020"
     CDS             2751..4502
                     /locus_tag="VLF64_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HSW90750.1"
                     /translation="MTDDIQQKLITLLQEEGLVSAETIRMATERSTKENKPLLAMLAA
                     DGTIDNELLTHGIAQVSGVPYVNLTNTIVDQSILSLLPSDIAERFMAVPLAEVQNRLA
                     VAMIDANNVQAVDYLANRIQRPLKVFMASEAGVRHVLDQYKTDLSSVDVAARASQEDA
                     KTESSDIKTIVQDSPISRALSTILEYAVKARASDIHIEPLEKALKIRCRVDGVLREVM
                     QLPKSIEPALVSRIKILSNLKIDEHRIPQDGQFTVKVGNKEVDLRIAISPVVWGEQVV
                     IRLLDKTGNSFDLEQMGYAGRALRAIRKGIKRPNGMILTSGPTGSGKSTSLYALIKEI
                     KDDTVNIVTLEDPVEYKMDGVNQIQVNAEVGLTFASGLRSILRQDPDVVMVGEIRDGE
                     TASLAVQAALTGHLVFSTLHTNSSAGVLPRLLDMEIEPFLIASTVNTIIGQRLVRRVA
                     VKRDAYWSSPLETKSILDNVGHLLPKTKEEVAQVSADLGYKDLPLANQTAYTLVKGHD
                     TPASPRGYSGRAGVYEVLDVTEEIRELIVKRTTSGEIQRKAVEQGMITMRQDGYLKAL
                     QGLTTLEEVNRVTSDTA"
     gene            4514..5587
                     /locus_tag="VLF64_02025"
     CDS             4514..5587
                     /locus_tag="VLF64_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HSW90751.1"
                     /translation="METQNNLRIEVLLEEVVKKRASDLHIQVGLPPMLRVDGSLTPIA
                     GFNPLDEQQVEALVFAILDQDQQQILMKDKEFDFSFAFGTLGRFRVNAFHERGNLAAA
                     LRLIPNEIKSVTELGMPPIVMTFAEYPRGLVLVTGPTGSGKSTTLAALVDKINTEKSQ
                     HIITIEDPIEFTHKSKRSVVVQREVHYDTYSFSAALRSSLRQDPDVVLIGEMRDLETI
                     SAAITIAETGHLVFATLHTNSAAQSIDRMIDVFPPHQQPQIRSQLANILMAICSQRLI
                     PAIGGGRVVAAEILIANPAVRNVIREGKSHQLDAIIQTGADQGMQTMDRTLVSLVQSG
                     TITYDSAREFAVDLTEFERLMRG"
     gene            5593..>6136
                     /locus_tag="VLF64_02030"
     CDS             5593..>6136
                     /locus_tag="VLF64_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSW90752.1"
                     /translation="MRKFNYQARDKSSNEVISSMVQADSETAAAKLLIEQGLMPLNIS
                     EIDEDGSLFAKIANRVTVKDKIVFLRQLATLIGAGLPLAQSLRTVLEQTENKKMQRVV
                     EEIIGEVEGGHTLSDAFGKHPEVFDKIVIALVSAGEASGTLDDALKRVAAQKEKDAAM
                     MSKIRGAMIYPAIVLVVIMGV"
BASE COUNT         1644 a         1270 c         1599 g         1623 t
ORIGIN      
        1 gcaatcactc gtactgctta tgtatactaa gagctagtat gaatatatta aaagggttgg
       61 gcgacttctt tgcactcgat attggcacta atgctattcg ggttgttcaa ctcaaaaagg
      121 ctggggcggg gtatgcgctg gaacattacg ggtatgcgcc tattgatagc aagctaactg
      181 gcagcgattc tgaagaggct cgacgtaagg tcggtgaagt tattatgact gcagttggac
      241 agagtggtat taaatcgaag aatgttgtga ttggcctgcc atcaagcaag acattcacca
      301 caattattga tgtgcccatg gtgagcgaag cagaacttaa ggcgacgatg aagtaccaga
      361 ttgatcagta catccctatg tctgttgatg acgcaaaagt agactgggct ttgcttggca
      421 attcgcttcg tgcacagaat cagcaggaag tgcttctctc aagcgtcgcg cgtcagtata
      481 gtgaagatag acttgaactc gttgaggccc ttggatttaa tgtgattgcg gctgagcctg
      541 atcctattgc aatggtgcgt gcattgagtg tgactggggc aacgggggcc gtattgcagc
      601 ttgatatggg tgaaaactca acggatatcg cgattgtata cggtgatacg ccacgccttg
      661 tgcgtactat tccaactggt gttgggtcac ttgtaaagtc aacggttacg aatctgaacg
      721 ttcaagaaga tcaggcgcgt cagtttattc tcaaatttgg acttgccccg gaccgtcttg
      781 aaggacaagt gcttcgcgcg cttgaatcgg tgctcgataa ctttgctgcc gaagttgtaa
      841 agtcaattaa gttctttgag acacgctatc caagtattgc cgtaggtggt gtgattgtaa
      901 ctggatttgc tgcaacgatc cctcagttcg atcaatatct tgctggcaag gttggcaagg
      961 gtgtgcagct cggtaaccct tggcaaggta ttcagatggc tgaagctgac cagcgacagc
     1021 ctgcagctgt ggctatggaa ttttcgacag taattggtct agctaagcga ggggttgagc
     1081 agtgattcag attaacctcg ttcctgacgt taaacaggaa atgctccacg ctcagcgtat
     1141 gcgcaatgtt gcaatctcgc tttcgattgt aattggactt gttgcggttg gtattgttgt
     1201 ggcacttgca ttgatccttg gtggtcaggt ggcggtacag aagattgagg agggccagat
     1261 tactagtggg tatagtaaac tctcttcggt gtcagatctt gatagtgcac tgacgattca
     1321 aaatcagctt ggtacaattt cgagccaaaa tgacaagcgt acggttgatt cgcgactctt
     1381 ggatgtactt gcagcgatta atccgcccgc gccaaacgat gtgaaacttt ctaaggtaac
     1441 tctcgatccg acgcagaata cggttactct tgaaggctca gcggtgggag gctatcctgc
     1501 aacggacgta tttagaaaga ctatcttaaa tacgaagatt accgcgaagc agcagggtaa
     1561 cgataatgca gcagaaactc acctcacctc aaatgttaca ttgcaggata caagctacgg
     1621 agaagacgct acaggcgcaa aggtagtacg ttttacgatt atctttacct atccaaacgg
     1681 acttttcgat aaccgtatga gcgatgtaaa gattagtacg ccgagcgccc gtgtggatgt
     1741 cactgactca aaaaccttga ttccggatag tatgtttagt caggccgcaa cagatttgaa
     1801 ggagaacaaa taatggcatc tcaaatggta agcccgaagc gtcgagctat tgcagattca
     1861 agtcgtacga tgtttatatg ggtagcgggc atgtcggcaa ttgttggtat ttgtgtggtt
     1921 gtagctatct tcttggtgca acagattatg tttaaggtga aggttgttgg ggcaatgacc
     1981 gaaactgtga atacactgaa ttataacaat aaagtcgcca aagaccttac tagcaatgtt
     2041 gtggtgttac agacaaactc ggcgcttaat tccgtgaaag caaatggcga tgagaatgcg
     2101 ttgcaggttc cacttgatgc actcccagca gagcgcaact cgctcgctct tggtgcatcc
     2161 cttcagcagg tgttacttgc gggagtggac ggactttcta ttgattcgct atctgttgat
     2221 acagttgatt cgaatgcaaa tactaatatc gccgatacca gtgcaacggc tgataatacg
     2281 atttcgttac agatgcagat ttcatcgagt aatgtaaata ccattaaaag caccctcaca
     2341 cgactagaga actcgattag ggtcattgat attgatagct ttatcttgga gcgaagcgat
     2401 actacatacc aggcgacaat tactgcacac gcctactatc aaccgggtat taaattacaa
     2461 ctacagtcga agtcaattat gccgggaggt aaataatgaa gaagagcgat atagcaatgg
     2521 ttgcgcttat cgcgagcgtg tcagtaatga tttcgtttgt gatcgctaat cagatctcat
     2581 tcctaaaacc gcctcaaaag ggtgaggatg tgaaaacaat tgagaagatt aatccagatg
     2641 ttactgcacc agacactaaa gtttttaggg ctgatgcaat taacccaacg gttcaaacag
     2701 taattggcgg tggtcagtct gcgcaatagt aggagtttac tatggccctg atgactgatg
     2761 atatccaaca gaaactgatt acacttctcc aagaagaggg gctggttagt gctgagacta
     2821 tcaggatggc aaccgaacgc tctacaaaag agaataagcc acttcttgct atgcttgcgg
     2881 ctgatggaac gattgataat gaactcctta cgcacggtat cgctcaagta tcaggtgtgc
     2941 catatgtgaa tcttacaaac acaatcgtag accaatcgat tttatcactt ttaccatctg
     3001 atattgcgga gcgatttatg gctgtgccgc ttgctgaggt acaaaaccgc cttgcggttg
     3061 cgatgattga tgcaaataac gtgcaggcag tggattatct ggcaaatcgc atccagcgcc
     3121 cacttaaggt gttcatggcg tcggaggcgg gtgttcgtca tgtccttgac cagtacaaaa
     3181 ccgacctttc tagcgttgat gttgcggcgc gtgcttcaca ggaagacgca aaaactgaga
     3241 gcagtgacat taagacgatt gttcaggatt cgccgattag ccgagcgctt agtaccattt
     3301 tagaatatgc ggtgaaggcg agagcgagtg acattcatat tgagccactt gagaaggcgc
     3361 tcaagattcg ttgccgcgtt gatggtgtgc tccgtgaggt gatgcaactt ccgaaaagta
     3421 ttgagccagc actcgtaagc cgtattaaga tcttgtcgaa tctaaagatt gacgagcacc
     3481 gcattccgca agatggtcaa tttacggtaa aggttggcaa taaagaagtg gaccttcgta
     3541 ttgctattag cccagtggta tggggcgagc aagtcgtgat ccgtttgctt gataagacag
     3601 gtaattcatt tgatcttgag caaatggggt atgcaggtcg tgctcttcgt gctattcgta
     3661 aaggcattaa gcgaccaaat ggtatgattc ttacgagtgg cccgactggt agtggtaagt
     3721 cgaccagtct gtacgcgctt atcaaagaga taaaagacga tactgtgaat attgtgacac
     3781 tcgaagatcc agttgagtac aaaatggacg gcgtgaatca gattcaggtg aatgctgagg
     3841 ttggccttac ctttgcttcg gggcttcgtt caattctacg ccaagaccca gacgttgtga
     3901 tggtaggtga gattcgtgat ggtgagaccg ccagtctggc ggttcaggca gcacttacag
     3961 gacaccttgt gtttagtacg cttcacacaa attcatcagc tggtgtgttg ccacgactac
     4021 ttgatatgga aattgagccc ttcttgattg caagtacagt gaacacaatt atcggccagc
     4081 gtctggttcg tcgtgttgct gtaaagcgtg acgcgtattg gtcaagcccg cttgaaacga
     4141 agagcatcct cgataatgta gggcatttat tgccaaaaac aaaagaggag gtagcacaag
     4201 tttcagccga tcttggatat aaagacttgc cacttgctaa ccaaaccgct tatactttgg
     4261 tgaagggaca tgatacgcct gcttcgccac gtggctactc gggtcgtgct ggagtctatg
     4321 aggtattgga tgtcactgaa gagatccgcg agcttattgt gaagcgtacc acgagtggtg
     4381 agattcagag aaaggcagtt gagcagggta tgattactat gcgacaggac ggctatctca
     4441 aggctttaca gggccttacg acacttgaag aagttaaccg agtaacgtca gatacggcat
     4501 aaaggggaag gtaatggaaa cacagaataa tttacggatt gaggtactac tagaagaggt
     4561 cgtgaagaag cgagcctccg accttcatat ccaggtgggt ttgcctccta tgctgcgtgt
     4621 ggatggctca ctgacgccga ttgctggatt taatcctctt gatgaacagc aagttgaagc
     4681 gctcgtcttt gctatcttag atcaagatca gcaacagatt cttatgaaag ataaagaatt
     4741 cgactttagc ttcgcctttg gtacacttgg tcgtttccgc gtgaatgcat ttcacgagcg
     4801 tggtaatctt gcagcggcac tccgtttgat tccaaatgag attaaatctg ttaccgaact
     4861 tggtatgccg ccaattgtta tgacatttgc tgaatatcca cgcggacttg tacttgtaac
     4921 tggcccgacg ggcagtggta agtcgacgac acttgcggca cttgttgata aaattaacac
     4981 cgaaaaatct cagcacatta ttacgattga agatccaatt gagtttactc acaaatcaaa
     5041 gcgctcggtc gtcgtacaac gagaggtgca ttatgacact tattcattct ctgccgcact
     5101 tcgttcgagt ttgcgccaag acccggatgt tgtgcttatc ggtgagatgc gcgatcttga
     5161 gacgattagc gcagcaatca ctattgccga gactggccac ttggtgtttg caacacttca
     5221 tacgaactct gccgctcaga gtatcgatcg tatgattgac gtattcccac ctcaccagca
     5281 gccgcagatt cgctcacagt tggcaaatat tctgatggcg atctgtagcc agcgtcttat
     5341 accggcaatt ggtggcggac gagtggttgc cgctgagatt ttgattgcta accctgcggt
     5401 tcgcaacgtg attcgcgaag gtaagagcca ccagcttgac gctattatcc agacgggggc
     5461 cgatcagggc atgcagacga tggatcgaac actcgtgagc ttagtgcaga gcggtactat
     5521 tacctacgat agcgcacgag agtttgctgt tgatcttacg gaattcgaaa ggctaatgcg
     5581 aggataatat aggtgagaaa gttcaactat caggcacggg acaagtcgag taacgaggtt
     5641 attagttcga tggttcaggc cgacagtgaa acggcagctg caaagttgct gattgagcag
     5701 ggcttgatgc cattgaatat atcggagatc gacgaagacg gctcgctctt tgctaaaatc
     5761 gctaaccgag ttactgtaaa agataagatt gtctttttac gccaacttgc aaccttgatt
     5821 ggtgccggct tgccacttgc acagagtctg cgtacggtac ttgagcagac cgagaataaa
     5881 aaaatgcaac gtgttgtcga agaaatcatt ggcgaagttg agggcggaca tacactttct
     5941 gatgcatttg gcaagcatcc agaagtattt gataagatcg tgattgcact tgtgtcagcc
     6001 ggtgaagcct ctggtacgct tgatgacgcg ctcaagcgtg ttgcagcgca aaaagagaaa
     6061 gatgctgcaa tgatgagcaa aatccgtggt gcaatgattt accctgcaat tgtgctggta
     6121 gtgatcatgg gcgtga
//
LOCUS       DATIIX010000068         2879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_405263, whole genome shotgun sequence.
ACCESSION   DATIIX010000068 DATIIX010000000
VERSION     DATIIX010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405263"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="VLF64_02035"
     CDS             <1..1157
                     /locus_tag="VLF64_02035"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HSW90753.1"
                     /translation="RVIETFEGQPVAVGAQSAEANLLDRNIDVLVKDVPELLEFLGRS
                     LRAVRRSHAKHIQIPEWHKNIRVEPHATSRPLHFGAQGFLPALLDRQKPILTVQVERQ
                     RADVGKVGGTEQLMARYKEMTSGAHRLDAEFIMHEAFDSVVPLYNVAQFEELKKYPNI
                     IAGIASIAEQSFMVISDQPSYRVFSDTYVSKHAPNPGPEDFEYTTRMIEAAERWKLPL
                     VFMTDTLGALPTLAAERRGQSRAIARAITAASSHPYPVISVITGALGSGGGLATTPFG
                     RHTVMLDSALAFVSEPRSMASILYSTANPTEDQVSMTLATVRASAEDLRSQGLVDMIV
                     TDSDNPYQTVVDIRKAIIEGFEKQQGKSPRQLRADADKRLATKMTAEGRR"
     gene            complement(1243..1617)
                     /gene="rplL"
                     /locus_tag="VLF64_02040"
     CDS             complement(1243..1617)
                     /gene="rplL"
                     /locus_tag="VLF64_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HSW90754.1"
                     /translation="MADVKKLAEELVKLTVLEVNELKNVLKDEYGIEPAAAAVAVAGP
                     AASPADAADEKTEFTVTLKDAGSQKVAVIKAVKELTGLGLGEAKAIVDGAPAPVKEKV
                     SKDDAEAAKKALEEAGATVEVA"
     gene            complement(1822..2346)
                     /gene="rplJ"
                     /locus_tag="VLF64_02045"
     CDS             complement(1822..2346)
                     /gene="rplJ"
                     /locus_tag="VLF64_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HSW90755.1"
                     /translation="MAISKDKKQALVNELSELFSSAKATAVAKYQGLTVAELQELRRG
                     AREADVTIKVVKNRLVRVAMTDNKTYKEADSSALVGQLLYAFSDTDEVAPAKVLDTFG
                     KTHPGLELVAGFSGEGLTQNTADVTALASLPSKQQLIAEVVAQLLSPVHDTTNALSGN
                     LHALLDGVSEKATA"
     misc_feature    complement(2425..2544)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(2576..>2879)
                     /locus_tag="VLF64_02050"
     CDS             complement(2576..>2879)
                     /locus_tag="VLF64_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90756.1"
                     /translation="QDGQVGPDLRTEIQITTKDDRIENRIGKAAHTLYKLVKYLGISA
                     NSIDRENYAQDLALIHARKKHLGATSYDQLTSGGLARADSLYKELTTPVAAVSSVT"
BASE COUNT          688 a          659 c          722 g          810 t
ORIGIN      
        1 cgcgcgtgat tgagacattt gaagggcagc ctgttgctgt gggtgcgcag agcgctgagg
       61 ctaatttact tgatcgtaac attgatgttt tagtgaagga tgtgcccgag ttgcttgaat
      121 tccttggcag gtctttgcgc gcagtgcgtc gttcgcatgc aaagcatatt cagattccag
      181 aatggcataa gaacattcgt gttgagcctc atgcgacaag caggccgtta cattttggtg
      241 cacaaggctt cttgcccgcc cttcttgatc gccagaagcc aatactaaca gtacaggttg
      301 aacgtcaacg agcagatgtc ggcaaagtgg gtggcactga gcagttaatg gctcgttaca
      361 aagaaatgac gtcgggcgcg catcgccttg atgctgagtt tattatgcac gaagcctttg
      421 atagtgtcgt gccgctttac aatgtggcgc aatttgaaga gttgaagaaa tatccaaata
      481 ttatcgcagg gattgcatcg attgcagagc agtcgtttat ggtgattagt gatcaaccat
      541 cgtatagagt gttttcagat acgtatgtta gcaaacacgc gccaaatcca ggacctgagg
      601 attttgaata cactacgcgc atgattgaag cggcagagcg gtggaaactt ccacttgtat
      661 ttatgaccga cacactgggc gcattgccca ctcttgcagc cgaacgacgt ggccagtcgc
      721 gcgcaatcgc tagggcaatt actgcagcga gctcgcatcc ctacccggtg atttcggtta
      781 ttacgggtgc acttggtagt ggcggcggcc ttgctacgac gccatttggc cgccatacgg
      841 taatgctcga tagtgcactg gcctttgttt ctgagccgcg ctcaatggct tcgattttgt
      901 acagcacggc caaccctacg gaggatcagg tttccatgac gctagcaacg gttcgtgcat
      961 cagctgagga tttacgatct caggggcttg ttgacatgat tgttacggat agtgataacc
     1021 cgtatcaaac ggtcgtagac atacgcaagg caattatcga aggttttgaa aagcaacaag
     1081 gtaaatcacc gcgccagctt cgtgctgatg cggataagag acttgcgaca aaaatgaccg
     1141 cagaaggtcg ccgctagtaa ggtttactgc aaaaaataac accctgcgat ttgcagggtg
     1201 ttattttaca tttgaatgta gtcgttttag gttgtaatga acttaggcta cttctacggt
     1261 agcgccagcc tcttcaaggg ctttctttgc agcctcagcg tcgtccttag ataccttctc
     1321 cttgactgga gctggggcac cgtcaacgat agccttagct tcgccaagac caagaccagt
     1381 gagttccttc acagccttga tcacagcaac tttttgagaa cctgcgtcct taagggttac
     1441 agtaaactca gtcttttcgt cagcagcgtc ggcagggctg gctgcaggac cagcaactgc
     1501 aactgcagca gcagctggct cgatgccgta ttcgtcttta aggacgtttt taagttcgtt
     1561 aacttcaagt acagtaagct ttacaagctc ttctgcaagt ttctttacgt ctgccatggg
     1621 taatttcctt tcaataccgg cctgcatatt gcgcacattt tggacgccct gttccgtgct
     1681 tcctagatag tacgatacgt acaatctatg tcagctactc acaggtgatc ccaaactgta
     1741 ctgcaatctg caactctggt agttggtaga ttctagtttg taatgataga tgcgtagttt
     1801 cctacgcgga tgctttggtt gttaggctgt agccttttcg ctcacaccat ccaaaagcgc
     1861 gtgcaaattg cctgatagcg cgttggttgt gtcgtgaact ggtgaaagca gttgagcaac
     1921 cacttcggca ataagttgct gcttgcttgg aagactagca agtgcagtta cgtctgcagt
     1981 gttttgcgtt aaaccttcac cgctaaaacc agcgacgagc tcgagacccg ggtgggtctt
     2041 tccaaacgta tcaagtacct ttgcaggagc cacttcgtca gtgtcgctga atgcatagag
     2101 caactgacct acaagtgctg atgaatcagc ttctttgtaa gtcttgttgt cagtcattgc
     2161 cacgcgaacg aggcggttct ttacaacctt gatggttacg tctgcttcac gagctccacg
     2221 gcgaagctct tggagctctg caacggtcag cccttggtat ttcgcaacgg ctgttgcttt
     2281 tgcgctcgag aatagctcgc taagttcatt taccaaagct tgtttcttat ctttgctgat
     2341 agccatagat aaaaacctca ctttttagct aaattttgta atataacgac cacattgtta
     2401 atgttcgaca ccacttactt gataggcgga cccaaaaaat ctttgattca ctatcaaaga
     2461 ctgtaggaaa agaatgtaat tccctcggtg gcatgattaa gtgaatttca ccgccactgt
     2521 ctttggtaat gtctgctagt attatagcag atagaggtag atactacaag tagcactagg
     2581 tgacagatga gacagcggca acgggtgttg tgagttcttt gtaaagcgag tcggcgcgag
     2641 ccaggccacc gcttgtgagt tgatcgtagc tagtggcacc gaggtgtttt tttcgagcat
     2701 ggataagtgc gaggtcctgt gcgtaattct ctctatcaat agagttagca cttattccga
     2761 ggtatttgac tagtttgtat agcgtatgtg ccgctttgcc gatgcggttt tcaatgcgat
     2821 catctttggt ggtaatctga atttctgtac gcagatcagg tcctacctgc ccgtcttgt
//
LOCUS       DATIIX010000069         4415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_185083, whole genome shotgun sequence.
ACCESSION   DATIIX010000069 DATIIX010000000
VERSION     DATIIX010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185083"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /gene="rny"
                     /locus_tag="VLF64_02055"
     CDS             <1..1309
                     /gene="rny"
                     /locus_tag="VLF64_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HSW90757.1"
                     /translation="ERRKEIQKLENRLADRESNLDKKLDELDKRAEKLRKEETEVETL
                     KGEIREIRGRQQEKLEKIAGLKKKDAADKLMQMTERDIKDDLTGLVSKLQKEAVDDAE
                     ERAQLVIVGAMERMASEVTAERTVTAVKLTDDEMKGRIIGKEGRNIQAMQRATGVDFL
                     VDDTPGMVVLSSFDPIRRQVARIGLEMLMKDGRIHPGRIEEVFAKAEKQIEKDVIRAG
                     EDAAREVGVTGIPKELLQLLGELKYRTSYGQNVLMHSTEMAHMAGLIADEIGANVRVT
                     KTATLLHDIGKAVTHKVEGKHHHIGAELARKAGMPDEIVHAIEAHHDDIEATTPEALV
                     VRVCDAISAARPGARNISAENFAERMRDLENIAIGFKGIDKAYAISAGREVRVIVKPE
                     NVDDLSAIKLARDIANKIESTMQYPGTIKVNVIRETRAVEFAK"
     gene            1309..1779
                     /locus_tag="VLF64_02060"
     CDS             1309..1779
                     /locus_tag="VLF64_02060"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HSW90758.1"
                     /translation="MSDYSSNEFSSWLARLEKRASSAGVALYDANERVLIVKAHYKKY
                     WSFPGGIIDSGETPRQAAIRETHEEVGLSVNIDKLVFRLVVNRASSIAQTYQFVFESR
                     VDASVLGHVLIDNKEIESYALVSREDILHGNRVCSTSVIEWAKGTLGYLEQDFN"
     gene            complement(1782..2402)
                     /locus_tag="VLF64_02065"
     CDS             complement(1782..2402)
                     /locus_tag="VLF64_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HSW90759.1"
                     /translation="MLLIETRTKRRTYISTTLAIFAVVALLMGLYILSLVFAPAIAPM
                     VSHTSIDVKKLPAPAMKDDRIVIPKIGVNILYGTGTAALNAGAEWRYPDRGNPEKGGN
                     FIIAAHRLTIQPTPWATVEKSPFYNIDKLAIGDKIIIDYSGTRYGYNISKIFNVTPNQ
                     VEIEAPSETPELTLYSCDITGSATGRVVIIAEPMGKVALNGSQQTN"
     gene            complement(2425..3021)
                     /locus_tag="VLF64_02070"
     CDS             complement(2425..3021)
                     /locus_tag="VLF64_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90760.1"
                     /translation="MNTAHKTKRQTPKTIANVFGTLGYFSVLLQWTWVLLLLCYPLLT
                     VNHSFLLPDAPAEPFHPTHNAVTESPITTVAAITISAFVLIATAVVITRLPKQIGKRG
                     ARLTHQTADAILPIVTKHKVLPQKKRRALSYRIILLAKLTFIILPVIGLSWVVPSDPL
                     TVPVVWTIGLYCAGWSIIYFAVQQMIGFFAKVDTRRLW"
     gene            complement(3216..>4415)
                     /locus_tag="VLF64_02075"
     CDS             complement(3216..>4415)
                     /locus_tag="VLF64_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HSW90761.1"
                     /translation="AYMANNGNVEDYGNYDITNRPKDMAINSVVIHDTEGDLQSVLSA
                     FQDPTFYASVHYVVDTDGTIYQMVQDKNIAWHAGNWSVNMHSIGIEHIGHAATGSTDY
                     TPAMYHASAQLVKYLTDKYDILRDREHIIGHDNVPATTTDGIASMHTDPGPYWNWQNY
                     MAMIGAPVLPTSNLRHKSNMVTIAPVWPLNKLTVTGCTTNTQSCVPSGAHADSFIYLR
                     AEPNEDAAFFTDPVIGQGSTDINNNAARLFYGQTFAVADQKIDNHGIWYEVWVNGRTG
                     WFYSPWKAPTAFPASGEYVTPKSDSGAAVYGRAIPERSEYPTDLLATPPASWYIPTPT
                     PLVYTVAAGQRYKLIEEVPTDHFYAWASDSSFPYDHTVFHGATNYLEIQIGNRVGYVK
                     ADSVNIR"
BASE COUNT         1219 a          970 c         1100 g         1126 t
ORIGIN      
        1 agagcgtcgc aaagaaatac agaaacttga gaaccgtctc gctgaccgcg agtcgaatct
       61 cgataaaaag cttgatgaac ttgacaagcg tgccgagaag ctacgtaaag aagaaaccga
      121 agtcgaaact ctgaagggcg aaatccgcga aatccgcggt cgccagcaag agaaacttga
      181 aaagatcgct ggcctcaaaa agaaagacgc tgcggataag ctaatgcaga tgaccgagcg
      241 tgacatcaaa gatgacctca ctgggctcgt aagtaaactg caaaaagaag ccgttgatga
      301 cgccgaagaa cgcgcgcagc tagtgattgt gggcgcaatg gagcgcatgg cgagcgaagt
      361 tacagccgag cgaacagtaa cggctgtgaa attgactgac gatgaaatga agggtcgtat
      421 tattggtaaa gaaggtcgaa acatccaggc gatgcagcgt gcaactggcg tcgacttctt
      481 agttgacgac actcctggaa tggtagtctt gagctcgttt gatcctatcc gtcgtcaagt
      541 agcgcgcatt ggtcttgaaa tgctcatgaa agacggccgt attcacccgg gtcgtataga
      601 ggaagtattt gcaaaagctg aaaagcaaat cgaaaaagac gttattcgcg caggtgaaga
      661 tgccgcacgc gaagttggtg taacagggat tccaaaagag cttttgcaac tgcttggcga
      721 acttaagtac cgcacaagtt acggccaaaa tgtcctcatg cacagcaccg aaatggcaca
      781 tatggcagga cttatcgccg atgagattgg cgcaaatgta cgcgttacta aaaccgcaac
      841 gctactgcac gatattggca aggcagtcac acacaaagta gaaggtaaac atcaccacat
      901 aggtgctgag cttgcacgta aagctggtat gccagatgaa atcgtacatg cgatcgaggc
      961 tcaccatgat gacatcgaag caacaacgcc tgaagccctc gtcgtacgtg tctgtgatgc
     1021 aatcagtgcg gctcgtcctg gcgctcgcaa tatcagcgcc gagaatttcg cagaacgcat
     1081 gcgtgatcta gaaaacatcg cgatcgggtt caagggcatc gataaggcgt acgcgattag
     1141 cgctggccgc gaagtccgcg taattgtgaa gccagaaaat gtcgatgacc tctcagccat
     1201 caagctcgca cgcgatatcg caaataagat cgaaagcacg atgcaatatc ctggcactat
     1261 taaggtgaat gtaattcgtg agacccgggc ggtagaattt gcaaaataat gagcgactac
     1321 agtagtaacg aattctcgag ttggctggcg cgtcttgaaa aacgtgcctc cagcgcgggt
     1381 gttgctctgt atgatgcaaa cgagcgtgtc ttaatcgtga aggcacacta taaaaagtat
     1441 tggtcatttc ctggcggtat cattgattct ggtgaaacgc ctcgacaggc ggcgattcgc
     1501 gaaacacatg aagaggtagg actgtcagtg aatatcgata agcttgtttt tcggctcgtc
     1561 gtaaacaggg ctagtagtat cgcacagacc tatcaattcg tttttgaaag ccgagttgac
     1621 gcgagtgtgc tcggccatgt gttgattgac aataaagaaa ttgagtccta tgctcttgtg
     1681 tctcgtgaag atattcttca tggtaatagg gtttgctcaa catcagtcat tgaatgggca
     1741 aaaggtacgc tcggatacct cgagcaagac tttaactaaa tttagttggt ttgctggctg
     1801 ccattcagag cgaccttgcc cattggctcg gcaataatca cgacacgccc cgtagcagaa
     1861 ccagtgatat cgcaactata taatgttaac tctggcgtct ctgacggcgc ctcaatctct
     1921 acttggtttg gtgtgacatt aaagatttta ctaatgttat atccgtaacg cgtacccgaa
     1981 tagtcaataa taattttatc cccaatggct agtttgtcga tattataaaa cggcgatttc
     2041 tcaaccgtag cccatggtgt tggctgaatt gttaatcggt gtgcggcaat aataaagttg
     2101 ccgccttttt cagggttgcc tcgatccgga tagcgccact cggcacctgc gttaagcgct
     2161 gcagtacccg tgccatagag aatattcacg ccaatttttg ggataacgat tcgatcatct
     2221 ttcatagctg gcgctgggag ttttttgaca tcgattgatg tatgcgaaac cataggcgca
     2281 atagctggtg caaatacgag cgataagata tatagaccca taagcagcgc taccacagca
     2341 aaaatggcta gtgtcgtaga tatataagtg cgtcgtttag tgcgtgtttc aataagcagc
     2401 ataagcaata taacgtatac tattctacca tagtcgacgg gtgtctactt tggcaaagaa
     2461 cccaatcatc tgttgaaccg cgaaataaat aatgctccaa ccagcacaat aaagtccgat
     2521 agtccacaca acggggacgg taagtggatc cgatggcacc acccaactga gcccaatgac
     2581 cggaaggata ataaatgtaa gttttgcaag aagaataata cgataggaca gtgcgcggcg
     2641 tttcttttgt ggtagcacct tatgtttagt aacaataggc agaattgcat ctgcagtttg
     2701 atgagtaagt cgcgcgccac gtttacctat ttgcttcggt agtcgtgtga taacaacggc
     2761 ggtagcaata agtacaaatg cgcttattgt aatggcagca actgtcgtaa ttggcgattc
     2821 cgttacagca ttatgtgtag ggtgaaatgg ctcggcggga gcatcgggaa gcaaaaaact
     2881 gtggttcact gtaagaagcg gatagcaaag aagcaacagt acccaggtcc actggagtaa
     2941 gactgaaaaa tagccaagtg tcccaaaaac attggcgatt gttttaggtg tttggcgctt
     3001 agtcttatgc gcggtgttca tgcttttatg gtaacagata ttaaaaggaa atgaacaact
     3061 cttatattaa tttttcctaa atataagcaa tatgattgct gactttgcag catttttcat
     3121 gtccatactt actatagcgt atccatgact catcatgaaa agaagtaccg tttatgatac
     3181 tcctttccat gtgcgatatc gtctatcttg tgaaactatc gtatatttac tgagtcggct
     3241 tttacgtatc caacgcgatt accaatttga atttctaggt agttcgtagc tccatggaaa
     3301 accgtgtggt cgtatgggaa cgaactatcg ctagcccatg cataaaagtg atctgtcggt
     3361 acttcttcaa taagcttata gcgttgaccg gcagctacag tgtagactaa tggtgttggc
     3421 gtggggatgt accacgaagc aggaggggtg gcaagcagat ctgttggata ttctgaccgt
     3481 tccggaatag cgcggccata tacggctgct cccgagtcag attttggggt tacatattca
     3541 cccgatgccg ggaatgctgt cggcgctttc catggactat agaaccagcc tgtacgtcca
     3601 ttgacccata cttcgtacca aatgccatgg ttatcaatct tctgatcggc aacggcgaat
     3661 gtttgaccat aaaacagccg cgcggcatta ttatttatgt ctgtgcttcc ctgcccaatg
     3721 accgggtcgg tgaaaaatgc tgcgtcttcg ttcggttcgg cgcgtaagta aatgaaactg
     3781 tcagcatgcg cgcctgaagg tacacaggac tgtgtatttg ttgtgcagcc agttaccgtc
     3841 agcttgttaa gtggccacac gggagcgatc gttaccatgt tacttttgtg ccgcaagtta
     3901 ctggtcggca gaaccggtgc accaatcatt gccatataat tctgccaatt ccagtaaggg
     3961 cctggatcgg tgtgcatact agcgatgccg tccgtggttg ttgccggtac gttatcatgg
     4021 ccaataatgt gttcgcggtc gcgaaggatg tcgtacttat ctgttaagta tttcacaagc
     4081 tgcgcactgg catgatacat tgctggcgtg taatccgtac tgccagtcgc ggcgtggccg
     4141 atgtgctcaa tgccgatact atgcatgttg actgaccaat tacccgcatg ccacgcgata
     4201 tttttgtcct gtaccatctg atagatcgta ccatccgtgt ctacaacata gtgaaccgaa
     4261 gcataaaatg tcggatcttg gaacgcacta aggacgctct gcaaatcgcc ttcggtgtcg
     4321 tgaatgacca cagagtttat tgccatatct ttcggacgat ttgtaatatc gtaattgcca
     4381 tagtcctcga cgttaccatt atttgccata tatgc
//
LOCUS       DATIIX010000070         3789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_350836, whole genome shotgun sequence.
ACCESSION   DATIIX010000070 DATIIX010000000
VERSION     DATIIX010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350836"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="VLF64_02080"
     CDS             complement(<1..1030)
                     /locus_tag="VLF64_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HSW90762.1"
                     /translation="MLDIRFIRENPDRVQEAAAQKGYTVNIAELLSVDESRRELQMKA
                     DELRTKRNEISAQMKGGKPSAELVEQGKVIKTELIEVEDHLHIVEETYIQLLKVVPNM
                     PLDTVPIGTSEDENVVIKTVGEKPEFAFVPKSHWEIAEARGWIDKERAAKIAGARFAY
                     LRGDLVRLEMALWQYGMDVLGSEEVLQQIIDNNQLTGVSNKPFVPIIPPAVARTEVFQ
                     ATGRLNKEEQTYKIEDEDLWLNASAEHTMAPMYMNEILPQAELPLRMVGYTTAFRREA
                     GTYGKDAEGIFRLHQFNKLEMESFSTAETSLNEHLFMVAIQEYLMTQLGLPFQTLEKC
                     TADIGRPNA"
     gene            complement(1094..2362)
                     /locus_tag="VLF64_02085"
     CDS             complement(1094..2362)
                     /locus_tag="VLF64_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSW90763.1"
                     /translation="MRRADILTRAGRNLRQAKVRTLLTALAISVGAFTITLALAAGTG
                     GKLYTDNLIKNNGDANSLTVFAKNSSQDSSGPRKYGADESHVSQGLLGDADVKKIQSL
                     AGVESVTPMYNVGATYMVGLNGEKYDTSVSIKVDQTGVTLGAGALTHNQPATGSVVIP
                     EDYIKPLGYGSDGAAIGKAITLHVDKTTIPGLPTEGKDISFTIAAVSKKSDTVLHYQA
                     EVLLNSADGKAIYDYQTPAQAANQYYGVNVRVANSADAKAVKTGIENKGDYQVFSLQD
                     VQQVLFTFVNVVMGGAAGFGALAILASIFGIINTQYISVLERTQQIGLMKALGARRKD
                     IGKLFRYEAAWVGFLGGLIGTVGALLVGLFNPMIATALNLDKGTELLVFQPLTSVILI
                     LALVLVAVLAGYFPSRKASRLDPIEALRVE"
     gene            complement(2359..3042)
                     /locus_tag="VLF64_02090"
     CDS             complement(2359..3042)
                     /locus_tag="VLF64_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSW90764.1"
                     /translation="MIEVKDITKVYGKKKNTFTALSNMSFIVPDGEVTAIVGKSGSGK
                     STLMHVMSGLDHATEGAVLVNGENLSLMKQKNIDAFRAHQMSFIFQSFFVQGNETCYD
                     NVSLPLEIANVPGKDRKAMIESALEAVEMSEKKKSKAKDLSGGQKQRLAIARAIVNRP
                     KILFADEPTGNLDSTTGDHVEKLLFNYARKNNANLIIVTHDPDLAARCDRRIYVKDGR
                     IEKMEGGKV"
     gene            complement(3081..>3789)
                     /locus_tag="VLF64_02095"
     CDS             complement(3081..>3789)
                     /locus_tag="VLF64_02095"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HSW90765.1"
                     /translation="VGFGVYGVNQIHQRMDQLKVSDTSKLSELVGVTGATVEDDSSVA
                     VKYVVAEGTPYATVTHLRGQNTAKVSIVKTGDTAAITIKDEGTCEYSEYECNSLSRVT
                     IYGPQLSQLTVGHGGLDYQTAKTNDLNVSVNSEAQLVLHGSVVSLTANVDDLATLSAS
                     DVAVQRVTLTMATSADVDFGTVSDLALSVPTACAVGRHGQLSTEGISRFTVNGQTASI
                     TGHHYDANTPCLLINHW"
BASE COUNT          988 a         1038 c          733 g         1030 t
ORIGIN      
        1 ttgcgtttgg ccgaccaata tcagctgtac atttctcaag tgtttgaaaa ggcaaaccaa
       61 gttgtgtcat taggtattct tggatcgcca ccataaacaa atgttcattt aatgatgtct
      121 ccgcggtcga gaagctctcc atttcaagct tattaaactg gtgaaggcga aaaattccct
      181 cggcgtcctt gccataagtt ccagcttcgc gacgaaatgc cgtcgtatat ccaaccatgc
      241 gaagtggcaa ctctgcctgt ggcaaaatct cgttcatata catcggtgcc atcgtatgtt
      301 cagcacttgc gttaagccac aaatcctcat cctcaatctt ataggtttgc tcttctttat
      361 ttaagcgacc cgtcgcctgg aaaacctccg tacgcgcaac tgctggtggg ataatgggaa
      421 caaatggctt gttactaaca ccagttagct gattattgtc aatgatctgt tgcagcacct
      481 cttcggagcc cagtacgtcc atcccatact gccatagcgc catctcaagc ctgacaagat
      541 cacctcgtaa gtacgcaaat cgtgcaccag caatctttgc ggcgcgctcc ttatctatcc
      601 acccccgagc ttcggcgatc tcccagtggc tcttaggaac aaaagcaaat tcaggtttct
      661 cccccactgt cttaataacg acattctcat cctcactagt accgataggt acggtatcta
      721 gcggcatatt aggcactact ttgagcaatt ggatatatgt ctcttccact atgtgtaaat
      781 gatcttccac ctcaataagc tcagtcttta tcaccttgcc ctgttcaaca agctctgcgc
      841 tcggcttgcc gcccttcatc tgtgcggaga tctcattacg cttcgtacgc aattcatccg
      901 ctttcatctg gagttcacgt cgcgattcat ccacactcaa aagctcggca atgtttaccg
      961 tataaccttt ttgcgcagcc gcttcctgga cgcggtcggg attctcccta atgaatcgga
     1021 tgtctaacat actgtaaatt atacactagt cactcccgaa cgataagagg tggccatagc
     1081 cacctctaga gtattattca acgcgaagtg cttcaatcgg atcaagccgt gacgctttgc
     1141 ggcttgggaa ataacccgca agcacagcca caaggacaag cgcaaggatg agaatcacac
     1201 tcgtcagcgg ctgaaacaca agtagctccg tgcctttatc aagattgagt gccgtagcaa
     1261 tcattgggtt aaagagcccg acaagcaatg cgcctactgt cccaataaga ccaccgagaa
     1321 acccaaccca tgctgcttca taacggaata gcttaccaat gtctttgcga cgagcgccaa
     1381 gtgccttcat tagaccaatc tgctgtgtgc gctctaacac gcttatgtac tgtgtattga
     1441 taataccgaa gattgaagca agaatcgcca gtgcaccaaa gcccgcagcg ccgcccatga
     1501 caacattgac aaatgtaaag agtacttgtt gcacatcttg caatgagaat acctggtaat
     1561 cacccttatt ttcaatcccc gtcttcaccg cttttgcatc tgcgctattc gccacacgaa
     1621 cgttcacacc ataatattgg tttgccgcct gcgcaggggt ctggtaatca taaatagcct
     1681 tgccgtcagc agaattaagc aacacctcgg cttggtaatg cagcaccgta tcagactttt
     1741 tgcttacagc cgcaatcgta aaacttatgt ccttgccttc tgttggcaaa cccggaatcg
     1801 tcgtcttatc gacatgtagc gtgattgcct taccgatagc tgccccatcc gaaccatagc
     1861 caagtggctt aatataatct tctgggatga ccaccgagcc agtcgctggc tgattgtgcg
     1921 taagcgcacc cgcaccgaga gttacacctg tttgatcaac tttgattgaa actgaagtat
     1981 catacttctc gccattcagt ccaaccatat acgttgcacc tacattatac attggcgtaa
     2041 cactttcaac tccagccaaa ctttggatct ttttaacgtc cgcatcaccc aagaggcctt
     2101 gagaaacatg cgattcatct gcgccgtact tcctcgggcc tgacgaatct tgtgaggaat
     2161 tctttgcaaa cacagtgagg ctgtttgcgt caccattatt cttaataaga ttatcggtat
     2221 ataatttacc acccgtacca gcggcaagcg cgagtgtaat cgtaaacgca cccacgctaa
     2281 ttgcaagtgc ggttagcaac gtgcggactt ttgcctggcg taagtttcgc cctgccctgg
     2341 tcaggatatc tgcgcgcctc ataccttgcc tccttccatt ttttcaattc tgccatcttt
     2401 cacatagatt cgacgatcac agcgtgccgc caaatctgga tcatgtgtca caataataag
     2461 attagcgtta ttctttcgcg catagttgaa taataacttt tcaacatgat cacccgttgt
     2521 cgaatcaagg ttacccgttg gctcatcagc gaacaaaatt tttggtcgat tcacaattgc
     2581 gcgcgcaatc gcaagacgct gcttttgacc acctgacaaa tctttcgcct ttgacttctt
     2641 cttttcgctc atttctacag cctcaagcgc cgattcgatc atcgctttcc gatccttacc
     2701 tggtacgttt gcaatctcca gtgggaggct aacattgtca tagcatgttt cgttaccctg
     2761 cacaaaaaag ctctggaaaa taaaactcat ctgatgcgca cgaaaagcat caatattttt
     2821 ctgcttcatt aggctgagat tttcgccgtt cacaagcacc gcaccctccg ttgcatggtc
     2881 caagccactc atcacatgca taagtgtact tttacctgaa ccagattttc caacaatcgc
     2941 cgtcacctct ccatccggta caataaaact catgttactc agcgcggtaa aagtattctt
     3001 ctttttacca tacaccttcg taatgtcttt tacttcaatc aacgtgaatc ctccaatgtt
     3061 cttacgctta gtataacaaa ttaccaatgg ttaatcagaa gacatggcgt atttgcatca
     3121 taatgatgtc ctgttatgct agccgtttgt ccgttaacag taaaccgcga tataccttcg
     3181 gtagagagtt gaccatgcct acccactgca caagcagtcg gcacacttag ggcgagatca
     3241 ctaacagtcc cgaagtccac atctgccgaa gtcgccatag taagtgtcac gcgctgaacc
     3301 gcaacatctg atgctgaaag agttgccaaa tcatcaacgt ttgcagtaag tgacacgacg
     3361 gagccatgca gtacaagctg cgcctcacta ttaacactaa cgttcagatc atttgtcttt
     3421 gccgtctgat aatcaagccc accatgacct accgtaagct gcgagagctg tgggccataa
     3481 atagtaacac gagaaagact attgcactcg tactcactat attcacaggt accctcatct
     3541 ttaatagtga ttgctgccgt atcacccgtt ttaacaatgc tcacctttgc cgtattctgc
     3601 ccgcgcagat gagtgacggt tgcataggga gtcccttcag ccacaacata cttcacagcg
     3661 acactactat catcttcaac ggtcgcacct gtaacgccaa cgagctcact cagtttactt
     3721 gtatcactaa ccttaagctg atccattctt tgatgaattt gattcacacc atacacacca
     3781 aagccaaca
//
LOCUS       DATIIX010000071         9777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_379409, whole genome shotgun sequence.
ACCESSION   DATIIX010000071 DATIIX010000000
VERSION     DATIIX010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 9777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9777
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379409"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="VLF64_02100"
     CDS             complement(<1..1065)
                     /locus_tag="VLF64_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSW90766.1"
                     /translation="MDSLKLSTLFKGRVIIPTDADYDAERQVFYGGADKKPAVIIKVA
                     NAEDIQKVIAFARDQNLELVIRSGGHSPYSATDGGIVIDLRDLHNVVVDEVGKTVWAG
                     SGLTAGELTTELDKYNLAIGFGDTGSVGIGGITLGGGVGFLARKFGLAIDNLLAAEIV
                     TADGKILQVDEDNYPDLFWAVRGGGGNFGVVTKFKYALHDLNGCYGGMLLMPATPEVI
                     TGIAQIAKKAPDELSVIVNVMPTFPAPFVAKEFHGKMSVMTLVMYAGDPKEGEKVISP
                     IRALAKPIADMLKPMRYKDIFMPEDQNYHPTAVSTNLFMEDIDRDTAVSILDWLGRID
                     ASMKACQFRVLGGAVARISDD"
     gene            1297..1434
                     /locus_tag="VLF64_02105"
     CDS             1297..1434
                     /locus_tag="VLF64_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90767.1"
                     /translation="MMGFSARRAANSSAKDARARKGKAAPKKMPPEQKKIRRRKRRKK
                     R"
     gene            1524..3080
                     /locus_tag="VLF64_02110"
     CDS             1524..3080
                     /locus_tag="VLF64_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90768.1"
                     /translation="MTEVLNASKEFSRSPEQFRDFMSQNPVYLISADLPGCGSTTLTR
                     GIAERVVTGYGIEPHVVQIGEALRQALGVSNEVELQEKLREIKDPAQFDPQFYGDLPD
                     DRPCIIDGKLATTVGLQYIDSQSRPVVRIDLTSEPLVSAKRVLQREGYQFKDIFAAQD
                     NGEALLARLAMIRRRANHDSDMRSSLHTEADDSSLHYKIDTSKISITEIIEYFTGTAD
                     FESQVPEWEFQALKETLATLAQLHIMLDGSVHPNDVAHFEHQFQSIRYNMDRLSVMTH
                     PAGIADVRASLKKSITDCWFGLMMKEAPRFFADEKGVPMVDTDSKSWTPEFYKVAEGW
                     PILSSLLKDKKILDPFAGAGTLINLLVARNIPQSAVLSDIAFNGGRPVDKKGHTYLPA
                     LNNQVSQLLFDNLPSWYKPDFTPIQGYVTASANQLPIRDNAFDYVVADPPYGRNCASG
                     GIGLLLGSINEFNRVATEGSILMVPVEWVKEIAAAGHTVTQLTTDVSRGHSKLPVCYI
                     LVESKKEIKS"
     gene            3095..3388
                     /locus_tag="VLF64_02115"
     CDS             3095..3388
                     /locus_tag="VLF64_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HSW90769.1"
                     /translation="MRDLQKHIADVCKEKGWDKNSIPEIYLLFSEECGELAKAIRKET
                     AFKGEKKPDTKDNLIEEFADVLNYLMDLANQFDIDLTDAYFKKHDINKDRRWL"
     gene            complement(3576..6020)
                     /locus_tag="VLF64_02120"
     CDS             complement(3576..6020)
                     /locus_tag="VLF64_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSW90770.1"
                     /translation="MEDLAFHYKSTRAGVARFGVLVHGAVAMLLPWLAALFIMCGVGI
                     VIGLSMALGWLFIAIGVIPYIFFAFYKYHVKTLPSGKERSITALLAGDVLGHLPASPT
                     PSDVARAVSLAPSGQFMAVRLGLTPSLLDSLASKDATTMPALWKRADEIRVKNGLSQI
                     TGSMLAVAIVRSFTGYQSVIAQMHLADEDLDGGVVWQQHLMDLVQKHTKPRRTGGIAR
                     DWSFGWIPLLQRFGQNISEQMSGSGGLLTVDVSSHIEAIDQLIETFGTGGRQNAVLVG
                     QAGSGKTTIIHAFAERLLDASTNLPNSLKFRQVFILDSAALISAAPGRGELENLIMQV
                     LNEAYTAKNIIICLDNAQLFFEEGVGAVDLSNVLQPILEAGNLRVILSMDEQKYLELG
                     RRNPSLVNALNRINIEQSDEVETKRVMQEQLIITEVRRNVTYMYQAVNEAYHLSERYM
                     HEMVMPGRAIRLLESAASFSESGLVTMNSVQQAIEKTLDVKVSVASEASDRERLLNME
                     DLIHKRMVNQVRAVSVVSDALRRARAGVRNQNRPIGTFLFLGPTGVGKTELSKALADV
                     YFGGEGKMVRIDLNEYVRPEDVTRLIADGVDDPSSLTARVMKQPFSVILLDEIEKAAP
                     EVLSTLLQMLDEGILRDAHNREVSFRDAIIIATSNAGADRIREYIERGYDVAQFEQQF
                     IDELITTNQFRPEFLNRFDEIVVFRPFKKEELVQVMDLILVGVNKTLEPQKVSVDVDD
                     EAKQILVDKGYDPRLGARPMRRVVQKAVENTVAKQMLSGAVNPGDVIHITGNQVAAIF
                     ATSETARAISDSNGGQ"
     gene            complement(6008..6391)
                     /locus_tag="VLF64_02125"
     CDS             complement(6008..6391)
                     /locus_tag="VLF64_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90771.1"
                     /translation="MFIVGLLSWWYGAGWLSCMARVRDNLMSVYDYFSFDLLLRTLFS
                     PFRQISAGKVRGPLGVQMRAFLDKLISRAIGGIIRTTVIIFGSVILSIAAIIGAIRLV
                     LWPIIPLLPIVFVFLALAGWVPWKI"
     gene            6483..7766
                     /locus_tag="VLF64_02130"
     CDS             6483..7766
                     /locus_tag="VLF64_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HSW90772.1"
                     /translation="MQQTFITILGKSVRMLARARGGGSALPGLFVEKIDPNFIQRTLS
                     PIPRGVVLISGTNGKTTTTKMVVELLEGQGLRVFTNRTGSNFTRGVVAALLGDVDMHG
                     ELSADIAVLELDEAYATHFVRSIQPRYSLFLNVMRDQLDRFGEIDTTAKLLHMVATHT
                     TETIVVNHDDPRLNSPTFTSDLNAKVASFNVAPTLHRLFPSDDGMRGAKTGGKAKISN
                     ITPITTLEAIDGQRATIRFDNKNHETSLKIKGIYNLQNAVGAIALVHEIMGSELDTKR
                     MLASLANVTPAFGRGETVIVNQLPCELVLVKNPSGFRLGLKSFESKDYATMIAINDSY
                     ADGRDVSWLWDVDFDSLKNEGVAEVSGVRAFDMALRLQYDEVIANKIEPDLAKALAAF
                     IADNKHRPKRIYCTYTAMLALRRELSKHTDVETIR"
     gene            7763..8488
                     /locus_tag="VLF64_02135"
     CDS             7763..8488
                     /locus_tag="VLF64_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HSW90773.1"
                     /translation="MNENKTINLLQLYPRDMNIYGDWGNTLVLKKRLEWYGYKVNLLE
                     YNPGDKFPTHVDLIIGGGGQDSGQSVIGSDLLAIGDKLKALANDNVPMLMVCGLYQQF
                     GHFFKTRTGEIIQGIGLLDIETIGGDERLIGNIVSQNDEFGLIIGYENHSGLTTLGPK
                     ATPLGKVIRGAGNNNQDETEGARYKNVIGTYLHGSLLPKNPLIADWLIEKAVMRKYGD
                     FSSTLIDDRFAEQARDIAGRRPR"
     gene            complement(8509..8979)
                     /locus_tag="VLF64_02140"
     CDS             complement(8509..8979)
                     /locus_tag="VLF64_02140"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HSW90774.1"
                     /translation="MTMQSVPYMAPDFTLQDESGRTHSLADYKGKWLVLYFYPKDETP
                     GCTTEACSLRDAATDMSELDAEIVGVSADNAVSHGKFIAKHHLNFTLLSDQKKTTIEA
                     YGAWGKKMFGKEGILRKTFIINPHGEVVKVYGQVTPLGHGEQVTAELKRLKEHL"
     gene            complement(9064..>9777)
                     /locus_tag="VLF64_02145"
     CDS             complement(9064..>9777)
                     /locus_tag="VLF64_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90775.1"
                     /translation="TLYQPTYGLTVSLTVVLIAFIAAIIWSIKKGGNVLRKHPDELSL
                     LGTYIGVPIVMLMGISLVKSMYTERYLSHVAISLVMLIGIAASAALVQHLKRSQRITV
                     IVIVYGTMLIGFCNIATTGNFNFQRNEHPSVKQASASVHDCSPGSQVVAADPYVMTEL
                     SYYVTGCQTYFVSQWPTLAGGYAPWSGSKYQIKHMSDITSSRVTYVYYGTPDQAMPAI
                     YKEVATHAYGSLNVTTYER"
BASE COUNT         2670 a         2386 c         2192 g         2529 t
ORIGIN      
        1 gtcatcagat attcgcgcca ccgccccgcc taacactcga aactggcatg ccttcatcga
       61 cgcgtcaatt cttccaagcc agtccaaaat gctcaccgca gtatctcgat caatatcctc
      121 cataaagaga tttgtactca ccgcagtcgg atggtaattt tgatcttcag gcataaagat
      181 gtctttatag cgcatcggct ttagcatatc ggcaattggt ttcgccaagg cgcgaattgg
      241 cgaaatgact ttttcacctt cttttgggtc gcctgcatac attacaagtg tcatcaccga
      301 catcttaccg tgaaactcct tcgctacgaa cggtgcggga aatgtgggca tgacatttac
      361 aatcaccgat agctcatcag gtgctttttt agcaatctgt gcaatacctg tgattacctc
      421 gggtgtagct ggcattagca gcatgccgcc ataacaccca ttaagatcat gaagtgcgta
      481 tttaaatttt gttacaaccc caaaattgcc gccaccaccc cgaaccgccc agaataaatc
      541 agggtaatta tcttcgtcta cctgaagaat cttaccgtct gccgtcacga tctcagcagc
      601 gagtagatta tcaattgcaa gtccaaactt tcgtgccaaa aatccgacgc caccgccaag
      661 cgtaatacca ccaatgccga ctgaacctgt atcgccaaag ccaattgcta aattatattt
      721 atcgagttct gttgtgagct cgccagccgt aagtcccgat cccgcccaga cagttttacc
      781 tacttcatcg accactacat tatgcaagtc acggagatca atcactatgc caccatctgt
      841 cgcactatat gggctatgtc caccacttct tatgacaagc tcgagatttt gatcccgtgc
      901 aaatgcaatt accttctgga tatcttcggc atttgctact ttaataatta ctgccggctt
      961 tttatcagcg ccgccataaa atacctgacg ctctgcatcg taatccgcat cggttggtat
     1021 aatgactcta cccttaaata atgtgctaag ttttagtgaa tccatacata gccagtataa
     1081 cagctggcgt gcacccattt ctatgcaaaa tagatactcg ccactacaag cataggatct
     1141 taacgacgat tcagttgcgc tatcgattaa tctaatgcgc tcatatcatt agatatcttt
     1201 taatatattg acattaactt gtttaaatat cattgttctg ctttaatatt aataagttcc
     1261 ggcaaagtcg gtgcgggaga gatgcggaag aggaggatga tgggattctc tgccaggcga
     1321 gccgccaaca gtagcgcaaa agatgcgcgc gcaagaaaag gcaaggcagc ccccaagaag
     1381 atgccgccag agcagaagaa gatccgccgg cgtaaaaggc gcaagaagcg ctaaagaaag
     1441 gctggggtct tgtccgagca gaccccagcc tttccagatc ttgtgcgtcc gcatctctca
     1501 cagttacatc ttaaggagtt gtcatgaccg aagtgctgaa tgcatcaaaa gagttttcaa
     1561 gatcacccga gcaatttaga gactttatgt cccagaatcc cgtatacctc atttctgctg
     1621 acctgcctgg ctgcggttcg acaaccttaa ccagaggcat agccgagagg gtggtcactg
     1681 ggtatggaat agagcctcac gtcgttcaaa ttggagaagc tctgcgtcaa gctcttggcg
     1741 tgagcaatga agtagaactg caagagaagt tgagagaaat taaagaccct gcgcaatttg
     1801 atccgcaatt ctatggggat ttgcctgacg ataggccatg cattattgac ggcaaactag
     1861 caacaactgt tgggctacaa tacattgact ctcaaagtcg ccctgttgtg cgtatcgatc
     1921 tcacgtccga gccgctcgtt agcgctaaac gcgtattaca gcgagaaggc taccagttca
     1981 aggatatatt tgctgcgcaa gacaatggcg aggccctcct tgcgcgacta gctatgatta
     2041 ggcgacgtgc aaaccatgat tcggatatgc gtagctccct gcataccgag gcagatgata
     2101 gttcgctaca ttataaaatt gatacaagca aaatatcgat aactgaaata attgaatact
     2161 tcactggcac ggcagatttc gaatcacagg ttccagaatg ggaattccag gctctaaaag
     2221 agactcttgc cacgcttgca caacttcata ttatgttgga cggctcggta caccctaatg
     2281 atgtagctca ttttgagcat caattccaaa gcatacggta caacatggac cgactgagtg
     2341 ttatgacaca cccagcggga attgctgatg tgcgtgcttc actcaagaaa tcaattacag
     2401 attgctggtt tggtcttatg atgaaagagg cgccccgctt ctttgcagac gaaaagggtg
     2461 tgccaatggt agatacggat tcgaaaagct ggacgcctga attctacaaa gttgctgaag
     2521 ggtggccaat attgagctca ttattgaaag ataagaaaat attagatccg ttcgcaggtg
     2581 ccggcactct catcaacctg cttgttgccc gtaacatccc gcaatctgcc gtattatctg
     2641 atatcgcgtt caatgggggg aggcctgtcg acaaaaaagg ccacacctac cttcccgccc
     2701 tcaacaatca ggtttctcag ctgctttttg acaatcttcc ttcttggtat aagccggatt
     2761 tcacgccaat ccagggctac gttacggcta gcgctaatca gcttccgatt cgagataatg
     2821 ctttcgacta tgtcgtggcc gatccccctt atggccgtaa ttgtgcatca ggtggaatag
     2881 gactactgct cggtagtatc aatgagttta atcgagtcgc tacagaaggt agtattttga
     2941 tggttccagt agaatgggtc aaagaaattg ccgctgctgg acatactgtt acacaactca
     3001 ctacagatgt ttcgagaggt cattcgaagc taccggtatg ttatatacta gtagaaagta
     3061 aaaaggagat taaatcgtga acaacaatgt aacgttgagg gatttacaaa agcacattgc
     3121 tgatgtatgt aaagaaaaag gatgggataa aaattctatt ccagaaatct acctcctctt
     3181 tagcgaagaa tgtggtgaac ttgcaaaagc tataaggaaa gaaacggcat tcaaaggtga
     3241 gaaaaaaccc gatacaaaag ataatctcat cgaagaattt gctgatgttc ttaattatct
     3301 aatggatctc gcaaatcagt ttgatattga tcttacagat gcttacttca aaaaacacga
     3361 catcaataaa gatcgcaggt ggctgtaata tactaagcag atgcctaatt taaatactcg
     3421 ccctttaaag agcgagtatt ttttatgcaa gttgtagctt tttcttgaga gctttgagct
     3481 tcttcttgcg tgcacgcttg atatccgcgc cgtgccagtt gcgatgttta gccataatat
     3541 tctcctaact tgcacaagta taccatatat ctctcctact gaccaccgtt actgtcacta
     3601 attgcccgtg ctgtttcact tgttgcaaat attgccgcca cctggttgcc ggtaatatga
     3661 attacgtccc caggatttac tgcgcctgat aacatttgtt tagcgactgt attttcgacg
     3721 gctttttgca cgacacggcg catagggcgg gcgccgagcc gcggatcata ccctttgtct
     3781 acaagaatct gcttcgcttc atcgtctacg tcaactgata ctttttgtgg ttcaagcgtt
     3841 ttgttgacac ctacgagaat gaggtccatc acctgaacaa gttcttcttt cttaaagggc
     3901 ctaaatacga caatctcatc aaagcggttc aagaactctg gcctgaattg attagttgta
     3961 ataagctcat caataaattg ttgctcaaac tgtgcaacat cgtagccgcg ttcaatgtat
     4021 tcacgaattc gatcagctcc ggcattgctc gttgcaataa tgattgcgtc acggaaactt
     4081 acctcgcggt tatgagcatc gcgcaagatt ccttcgtcaa gcatctgcaa cagtgttgag
     4141 agaacctcag gcgccgcctt ttcaatctcg tcgaggagta ttacgctgaa cggctgcttc
     4201 atgacccgtg ccgtaagact gctcgggtca tcgacgccat ctgcaattaa gcgtgtaacg
     4261 tcctcggggc gtacatactc gttaaggtcg atgcgcacca ttttgccttc accaccaaag
     4321 tatacatctg caagcgcttt ggagagctcg gtcttgccca ctcctgttgg gccaaggaat
     4381 aaaaatgtac caattgggcg gttctgattg cgtacgccag cccgcgcacg gcgcaaagcg
     4441 tcactcacca cactcactgc acgcacctga ttcaccattc gtttatggat tagatcttcc
     4501 atattaagca atcgctcacg atcacttgct tcactggcga cactcacctt tacatccaag
     4561 gttttctcaa tcgcctgctg gactgagttc attgtgacca aacctgactc gctaaagctt
     4621 gccgcacttt ccagcaatcg tattgcgcgg cccggcataa ccatctcatg catatagcgc
     4681 tcactcaggt ggtatgcctc attcactgct tgatacatat acgttacgtt acgacgcacc
     4741 tcagtaataa taagctgctc ctgcattact cgttttgtct cgacctcgtc tgactgttca
     4801 atgtttatac gattaagcgc atttacaagc gaaggattac gccggcctag ctcaaggtat
     4861 ttttgctcat ccatcgataa gatgacacgc aagttaccgg cttccaagat tggctgcagc
     4921 acattcgata gatcaactgc tccgacacct tcctcaaaga ataactgcgc attatcaaga
     4981 cagataataa tgttcttggc ggtatatgct tcattcagca cctgcattat gagattctca
     5041 agctcgccac gaccaggtgc agcgctaatt aatgcagctg aatccaggat gaatacttgc
     5101 ctgaatttaa gtgagtttgg cagattcgtt gatgcatcga gcaatcgctc ggcgaatgca
     5161 tgaataatcg tggtcttgcc cgagccagcc tggccgacga gcactgcatt ttggcgccct
     5221 cctgtaccga acgtctcgat aagctgatcg attgcttcaa tgtgactgct cacatcaacc
     5281 gtcagtaacc ctccactacc gctcatttgc tcactaatat tctgcccgaa tcgctgtagc
     5341 aatggtatcc agccgaatga ccagtcacgc gcaataccac ctgtacgacg aggctttgta
     5401 tgcttttgga ctagatccat gagatgctgc tgccaaacga ccccaccatc gagatcttca
     5461 tcagctaaat gcatctgcgc aataactgac tgatacccag tgaaactacg gacgattgct
     5521 accgcaagca tgctaccggt aatttgagaa agtccgtttt taactcggat ttcatcggct
     5581 cgtttccata atgctggcat cgtcgtcgcg tctttgctcg ccagcgaatc gagcagcgat
     5641 ggggtgaggc caagccgtac agccataaat tgtccactcg gtgctaaact caccgcacga
     5701 gctacatccg atggagtcgg tgatgctggt aagtgtccga gtacgtcgcc tgcaagcaat
     5761 gcagttatag aacgctcttt gccacttggt aaggtcttta cgtgatattt atagaatgcg
     5821 aagaaaatat atggaataac accgattgct atgaataacc agccgagtgc catgctgagc
     5881 ccaataacaa tcccaacacc gcacataatg aaaagggctg ccagccaggg tagaagcatt
     5941 gctaccgctc catggactag taccccgaaa cgagcaacgc ctgcacgggt agatttgtaa
     6001 tgaaatgcta aatcttccat ggaacccacc ctgcaagtgc gagaaataca aaaacgatcg
     6061 gtaataatgg aataattggc caaagcacca ggcgaatagc gccaataatt gctgcaatag
     6121 agagtattac tgacccaaag ataatcactg tagtgcggat gataccacca atggcgcgtg
     6181 atattaattt atccaagaat gcgcgcattt gtacaccaag gggccctcgc accttacccg
     6241 cggaaatctg gcgaaatggt gagaataacg tacgaaggag tagatcaaac gaaaagtagt
     6301 cgtaaacact catgagatta tcgcgcacac gagccataca actaagccag ccggcaccat
     6361 accaccacga gagcagaccc actataaaca taatcttatt gtaccgtagt ggcaagatat
     6421 tatgaaatca gaatcgcgcc caagcttacg ttttgcctgt tttcaaatga tatactgaga
     6481 aggtgcaaca aacgtttatt actattttag gtaaaagtgt ccgaatgctc gcccgcgcca
     6541 ggggcggagg atcggcgtta ccggggttat ttgttgaaaa aattgacccg aactttattc
     6601 aacgtacatt atcgccgatc ccccgcggag tagttcttat tagtgggacg aatggcaaaa
     6661 ccacaaccac taagatggtt gtagagctac ttgaaggcca gggtcttcgt gtatttacga
     6721 accggactgg cagcaacttc acacgcggtg tcgtagcagc cttgcttggt gatgtcgata
     6781 tgcacggtga acttagcgcc gacattgccg tgctcgagct cgacgaagca tatgcaactc
     6841 actttgttcg cagcattcaa ccacgctaca gcttgttttt gaatgttatg cgtgatcagt
     6901 tggaccgctt tggcgagatt gatacaactg cgaaactgct acacatggtg gccacccata
     6961 ctactgaaac gattgtcgtg aatcacgatg atcctcgcct caacagcccc accttcacga
     7021 gcgatttgaa cgctaaggtt gcaagcttta atgttgcgcc gacactccat cgtctctttc
     7081 cgtcggatga tggcatgcgg ggcgcaaaga cgggcggtaa ggcaaaaatc tcaaatatca
     7141 caccaattac tactcttgaa gccattgatg ggcaacgcgc gactatacga tttgataata
     7201 aaaaccatga gacatcacta aagattaagg ggatctataa cctacagaat gctgtcgggg
     7261 ctattgcgct cgtacacgag attatgggta gcgagcttga taccaagcgt atgcttgcga
     7321 gccttgcaaa tgttacccca gcctttggac gtggtgaaac tgtcattgtt aaccagttgc
     7381 cgtgtgagtt ggtgctagtc aaaaacccaa gcggattcag gctcggtctc aagagctttg
     7441 agtcaaaaga ttacgcaaca atgattgcta ttaatgatag ctacgcggat gggcgcgacg
     7501 taagctggct atgggatgtt gattttgaca gtctcaaaaa cgaaggtgtt gccgaagtaa
     7561 gcggggtaag ggccttcgat atggcgttgc gcctacaata cgatgaagtt attgcaaata
     7621 agattgagcc agaccttgct aaagcgctcg cagcgtttat tgctgataac aagcacagac
     7681 cgaagcgtat ttactgcact tacactgcaa tgcttgcatt acgccgagag ctctcaaagc
     7741 ataccgacgt ggagacgatt cgatgaacga aaataagaca attaatctac ttcaactcta
     7801 ccctagagac atgaatatct acggcgactg gggcaatacc cttgtattaa aaaaacgcct
     7861 cgaatggtac ggctacaaag tgaatctact tgaatataac cctggcgata aattccctac
     7921 ccatgttgac ttaatcattg gcgggggcgg acaagatagc ggccagagtg ttattggctc
     7981 agatttacta gcaatcggcg acaaactgaa ggcacttgcg aacgacaatg tgccgatgct
     8041 aatggtatgt ggtctctacc agcaatttgg tcatttcttt aaaacacgca ccggcgaaat
     8101 catccagggt attggcctac tcgatattga gactatcggc ggtgatgaga ggcttattgg
     8161 taatatcgtc tcgcagaatg atgagttcgg actgattatt ggctatgaga atcactcagg
     8221 ccttacgacg ctcggcccga aggctacgcc actcggtaaa gttattcgtg gcgccggcaa
     8281 taacaatcaa gacgaaactg agggcgcacg ttacaaaaat gtcattggca cctacctgca
     8341 tggctcgtta ctgccaaaga acccactaat tgcagactgg cttattgaaa aggccgtcat
     8401 gcgtaaatac ggtgacttct cgtcgacgct tattgatgac cgctttgccg agcaagcgcg
     8461 cgatatcgcc ggccggcgac cgcgctagtt tattctagaa acacctcgtt acagatgctc
     8521 tttgagacgc ttgagctcag ctgtcacctg ctcgccatga ccaagcggcg tcacttggcc
     8581 atacaccttc acgacttcac catgagggtt aataataaag gtcttacgca agatgccctc
     8641 cttaccgaac atctttttgc cccatgcacc gtatgcctca attgtggtct tcttttgatc
     8701 tgataatagc gtgaaattaa ggtggtgttt tgcgataaat ttaccatggc tcacggcatt
     8761 atcggcgctc acgccaacaa tttcagcatc aagttcactc atatcggttg ccgcgtcacg
     8821 caagctgcag gcttcggtcg tgcagcccgg tgtctcatcc ttaggataga aatacaatac
     8881 taaccatttg cctttgtagt cggcaaggga atgagttctg ccagactcgt cttgaagtgt
     8941 aaaatctggt gccatatagg gaactgattg catcgtcata cctttagcgt actcctaaaa
     9001 caccaagctg tcacgtacgg atcgtatgtt gcaatgggcc tataatgtct catttcaacc
     9061 acactaacgc tcataggtgg tgacgtttaa tgagccatac gcatgggttg cgacttcttt
     9121 gtagattgct ggcattgctt ggtctggcgt cccgtagtac acataagtaa cacgcgagct
     9181 cgtaatatca ctcatgtgtt tgatttggta tttactaccc gaccatggtg catacccgcc
     9241 ggcgagtgtt ggccactgac taacaaaata ggtttggcaa cctgttacat agtagctcag
     9301 ctcggtcatt acatatggat cagccgctac cacttgggag cctgggctgc aatcatgaac
     9361 acttgcagat gcttgtttga ccgatggatg ttcattacgc tgaaagttga agttacccgt
     9421 tgtggcaata ttgcaaaagc cgataagcat cgtgccgtaa acgataacaa tcaccgtaat
     9481 ccgctgcgag cgcttaagat gttgcacgag tgccgctgac gctgcaatac cgattaacat
     9541 aacgaggcta atcgcaacat gtgacagata gcgctcggtg tacatagatt ttacaagcga
     9601 gatgcccatt aacatcacaa ttggtacacc aatgtacgta ccgagcagcg atagctcgtc
     9661 agggtgcttg cgtaagacat tcccaccttt tttaatagac caaataattg cggcaatgaa
     9721 tgctatgagc accaccgtga gtgataccgt taacccatat gtcggctggt aaagcgt
//
LOCUS       DATIIX010000072         2707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_240857, whole genome shotgun sequence.
ACCESSION   DATIIX010000072 DATIIX010000000
VERSION     DATIIX010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240857"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VLF64_02150"
     CDS             complement(<1..632)
                     /locus_tag="VLF64_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90776.1"
                     /translation="MKKLRLLIGSVAAFAVLAIAGLLYIPHADAGQCLSSSVMKCGFT
                     SVADMRNKYNSDTTPGTKNIYTYFGITSNTVNHYTTKDGTVTKNGDVYIGSEKVATGA
                     MTADRIDIAGSNRATKRTYNGTIFYTRTTEIAPSRAASAKVIVFLDGNGEFAGAVVYD
                     CGNPISGHPVPKPKPPVQACNLDTMKIETVDQSKIDNVHYTTDLSKCAPKP"
     gene            775..1326
                     /locus_tag="VLF64_02155"
     CDS             775..1326
                     /locus_tag="VLF64_02155"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSW90777.1"
                     /translation="MRSLHLTRPRAIMMVGVPGSGKSFFASQFSHTFSTPFIDSETIN
                     AITGDRVASGRIIALLLIEICKTNQTFILEGNSETKTLRTEFAEFAKRHGYDPLFIWV
                     QADPIISRQRSAKKGRNKESYESTLRRFEAPADAEKALVVSGRHTYATQVRMVLNHIG
                     NDNRPPIAHERQPSPVVPVRKRL"
     gene            1330..2028
                     /locus_tag="VLF64_02160"
     CDS             1330..2028
                     /locus_tag="VLF64_02160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HSW90778.1"
                     /translation="MSSFIDPEFGEIIVHKRRGMHSVHIKIGTDGRYAATAPFYTPLI
                     FIKQVVNNAREDLRKLAAHTSTARPYENGQAIGKTHTLAVVPTQTVKNPTTKILRERL
                     LVYLPPTSKLEDIAVQQQIRDTVVNILRQEAKAYLPNQLATLAKRHGFRYERIRFSHA
                     SGRWGSCSSTGTISLNIALMKLPDELIDYVLIHELCHTKHMNHSRRFWAEVEAINPRY
                     RLHKIQIAAHSPGV"
     gene            2072..>2707
                     /locus_tag="VLF64_02165"
     CDS             2072..>2707
                     /locus_tag="VLF64_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90779.1"
                     /translation="MKQGMGAILHINAHTERTIRLAGFVVPVILSLYGWLLQGDIIKT
                     NRVINPTLFYCISAAWITLGLYNWLSAVNSHIDAAVRLLLYHALAVAYVLFISGLAMP
                     FTGCAVVLFLASYTYFGRDGLNLSVLVLFVAMLSDMMLHMDDTQYMLQSGLFALGALV
                     VGFVTGTVVASQEIDRLELARNQAEKILQRDRILTIVNNLADAVISTDKDGV"
BASE COUNT          735 a          679 c          581 g          712 t
ORIGIN      
        1 ggctttggtg cacacttaga aagatcagtc gtgtaatgca cattgtcgat cttcgactgg
       61 tcaacagtct cgatcttcat agtgtcaagg ttgcaagctt gtacaggtgg ctttggtttt
      121 ggtaccggat ggccactgat agggttacca cagtcatata cgacagcacc ggcaaattca
      181 ccgttgccat caaggaacac aataaccttt gcgcttgcag cacgacttgg tgcaatttca
      241 gttgtacgag tatagaagat agtgccgttg taagtacgct tagtcgcacg atttgaacca
      301 gcaatatcga tgcggtcagc ggtcatagcg ccagtagcaa ctttttcact accaatatat
      361 acatctccgt tttttgttac ggtaccgtct ttcgtagtgt agtggttcac agtgttactg
      421 gtaataccaa agtatgtgta gatattcttc gtacctgggg tggtatcact gttatattta
      481 tttcgcatgt cagccacgga agtgaatcca cacttcataa ctgagcttga cagacactga
      541 ccagcatctg cgtgcggtat gtagagcaag cctgcaatag caagtacagc aaacgccgct
      601 acactaccga ttaataatcg caatttcttc atacacgttc ctccctgaac ttagtgtact
      661 ttgataaatt taattgtact ccttttttta agttttgtca atgttattat atagctatac
      721 ttcttatgct tatctcatct gacaaactca cattttttct gctacaatgg acatatgaga
      781 tcgttacacc ttacgcggcc gcgagcgatt atgatggtag gggtacccgg cagcggtaaa
      841 agcttctttg cgagtcagtt ttcgcataca tttagcacgc cgtttattga tagtgagacg
      901 attaatgcaa tcactggtga ccgagtcgcc tcgggccgca ttattgcact tcttttaata
      961 gaaatctgca aaaccaatca gaccttcatc ctagaaggca acagcgagac aaaaacactc
     1021 cgcaccgaat ttgcagaatt cgccaaaagg catggctatg atccactttt tatatgggta
     1081 caggctgacc caattatttc gcgacaacgc tcagcaaaaa aaggtcgaaa caaagagtca
     1141 tacgagtcaa cattgcgtcg attcgaagcg cctgccgatg ccgagaaagc actggtcgtc
     1201 agcggccgtc atacctatgc aacacaggta cgaatggtac tcaatcatat cggcaatgac
     1261 aaccgtccac cgattgcaca cgagcgtcag ccctcgccag tagtgccagt tcgtaaacgc
     1321 ttatagtata tgtcatcatt cattgacccc gaatttggcg aaattattgt tcacaaacga
     1381 cgaggcatgc acagcgtgca cataaaaatc ggtaccgatg gtcgctatgc cgccacagca
     1441 cctttctaca caccgctcat ctttattaag caagtcgtca ataatgcgcg cgaagatctc
     1501 cgaaaactcg cagcacatac gtcgactgct agaccctatg aaaacgggca agcaatcggc
     1561 aagacgcata cgcttgccgt cgtaccgacg caaactgtca aaaacccaac aaccaagatc
     1621 ttgcgcgaac gtctccttgt gtatctgcct ccaacgagca aactcgaaga catagccgta
     1681 caacagcaaa tccgcgacac tgtcgtaaac atcttacgcc aagaagccaa ggcatatctg
     1741 cccaatcaac ttgcgacact cgctaaaaga cacgggttta ggtacgagcg tattcgtttt
     1801 agccatgcga gtggccgatg gggaagctgc agcagcaccg gtacaatcag cctcaatata
     1861 gccctgatga aattgcctga tgagttgatt gattatgtgc tcatacatga actctgccac
     1921 accaagcaca tgaaccattc cagacgtttc tgggccgaag tcgaagcaat caacccccga
     1981 taccggctgc ataaaatcca gattgccgcc cactcaccag gtgtataaaa agcaagtgct
     2041 tttcgccggg caacatgata caataacaac aatgaaacag gggatgggtg cgatactaca
     2101 tatcaacgcg catactgagc gcacgatacg tcttgccggc tttgttgtcc ctgtaattct
     2161 atccctttac ggatggctac tacaaggtga tatcatcaaa acaaatcgtg ttatcaaccc
     2221 gacattattc tattgtatta gtgcggcctg gattacactc ggtttatata actggctttc
     2281 agcggtgaat tctcatatcg atgcggcagt ccgtctattg ctctatcacg cgcttgcagt
     2341 tgcatatgtt ttattcatat ctgggctcgc tatgcctttt actggctgtg cggtagtgct
     2401 cttcctcgcc tcatacacat attttggtcg tgatggactt aaccttagcg ttctggtatt
     2461 attcgtcgcc atgctaagcg acatgatgct tcatatggac gacacacagt acatgctgca
     2521 atctgggcta ttcgctcttg gcgcgcttgt cgttgggttt gtcactggca cagttgttgc
     2581 ttcacaagaa atcgatcgcc tcgaacttgc acgcaaccaa gccgagaaaa tcctacaacg
     2641 cgatcgcatt ctaacaatcg taaacaacct cgctgacgct gtcattagca cggataaaga
     2701 cggcgtg
//
LOCUS       DATIIX010000073         1778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_102564, whole genome shotgun sequence.
ACCESSION   DATIIX010000073 DATIIX010000000
VERSION     DATIIX010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102564"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VLF64_02170"
     CDS             <1..464
                     /locus_tag="VLF64_02170"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HSW90780.1"
                     /translation="TERPGIGLFLPIADCVGTIIYDPNRKALALAHLGRHASLADTIT
                     KTIEFFARKGSDPQDLHIWMAPSVKQQSYRMEYFDKAADPTWKEYCLKKSDGFYLDMQ
                     GYNQSRAITAGVSASNIVVSPVDTATNKHYFSHSQGDREGRFAVIAQLRAI"
     gene            complement(457..999)
                     /locus_tag="VLF64_02175"
     CDS             complement(457..999)
                     /locus_tag="VLF64_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HSW90781.1"
                     /translation="MKISRSGFTIVELLVVITVIGILATITVVSYNGVHARTRDDRRR
                     TDVANIIKAMELYYSDNNSYPTPLTNTGSAINNGWYSSGDTSWSKLSTLMLGSQAIDV
                     VPVDPINTPSGVPSAGGLVYAIYVNYNGGYCGATAGQMYMLVYKLEASTQVQKTDGTC
                     STNPLPSSYYSGLSYYQVVK"
     gene            1046..>1778
                     /locus_tag="VLF64_02180"
     CDS             1046..>1778
                     /locus_tag="VLF64_02180"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HSW90782.1"
                     /translation="MSKKAYITTAIPYVNGVPHIGHALDYTLADIWARYQKQNGHDVR
                     FQTGVDEHGNKIAAKAAENGLDPQSYVDKMHVNFQSLITSLNVESTDFIRTTDPHHKG
                     AVQYIWQKLTEAGLIYKGSYEGWYCTGCENFVTDKEATENNGICPDHQKPYERLSEEN
                     YYFKASHFSEQIREAIETNRLRIVPEFRKNEFLELIKDGIKDVSVSRPRKNLSWGVTV
                     PGDESQVMYVWLDALSNYITVLGYPD"
BASE COUNT          502 a          430 c          410 g          436 t
ORIGIN      
        1 acaccgaacg gccagggatt ggtctttttt tgccaatcgc cgattgcgtc ggtacgatta
       61 tttatgaccc aaaccgcaaa gcattggcgc ttgcccatct tggccgccac gccagtcttg
      121 cagatacgat cacaaaaaca attgagtttt ttgccaggaa gggtagcgat cctcaagatt
      181 tgcatatctg gatggcgccg agtgttaagc agcaaagcta tcgtatggag tattttgata
      241 aggccgcaga tccaacctgg aaagagtatt gtctcaagaa gagtgatggc ttttacctag
      301 atatgcaagg atataaccag tcacgcgcaa ttactgccgg tgtatccgct agtaatattg
      361 ttgtttcgcc ggtagatacg gccacaaaca agcattattt ttcacattca cagggcgacc
      421 gcgaagggcg ctttgctgtt attgcgcagc tccgcgctat ttaactactt gatagtaact
      481 gaggccacta tagtagctac tcggaagtgg gtttgtcgag cacgtgccat cggttttttg
      541 tacttgcgtg gaagcctcaa gcttgtagac gagcatatac atttggcctg ctgtagcgcc
      601 acagtaaccc ccattgtaat taacataaat ggcataaacc agccccccag cagacggtac
      661 acccgaaggt gtgttaatag ggtcaactgg cactacatca atagcctgcg acccgagcat
      721 aagggtagaa agcttgctcc agctcgtgtc accagagcta taccagccgt tattaatggc
      781 agatccggtg ttagtaagtg gtgtcggata gctattattg tcgctgtaat acagctccat
      841 cgctttgatt atattcgcta catccgttct gcggcgatca tcacgagtgc gcgcatgtac
      901 accgttgtaa gagacaacag tgatagtggc caaaatgcca atcacagtga tgacaacaag
      961 cagttcaaca atagtaaaac cgcttctgct tatcttcata gagctagtat accacttatt
     1021 tatcacctct ggtatactaa gagcaatgag taaaaaagcc tatattacta ccgcaatccc
     1081 gtacgtgaac ggcgtgccac atattggcca tgcgctagat tacacacttg ccgatatctg
     1141 ggctcgctac caaaaacaaa atggtcacga tgtccggttt caaacaggtg ttgacgaaca
     1201 tggcaacaag attgctgcaa aagctgccga aaatgggctc gatccgcaaa gctacgtaga
     1261 taagatgcac gttaatttcc aatcacttat cacctcgctc aacgtggaga gcactgattt
     1321 tatccgtaca accgaccccc atcacaaagg cgcagtgcag tatatttggc agaaacttac
     1381 tgaggccggg cttatttaca aaggaagcta cgaaggctgg tactgtactg gctgtgaaaa
     1441 ctttgttacc gacaaagagg caaccgaaaa caatggcatt tgcccagacc accaaaagcc
     1501 atatgagcgc ctgagcgaag aaaactacta cttcaaggcg agccatttct cagaacaaat
     1561 ccgtgaagca atcgaaacaa acaggttgcg tattgttccg gaattccgca agaacgaatt
     1621 cctcgagctc attaaagatg gcatcaaaga cgtatcggtg tctcgcccgc gcaagaatct
     1681 ttcttggggc gttacggtcc ctggggatga aagccaagtc atgtatgtct ggcttgacgc
     1741 gctttcaaac tacatcaccg tattaggata cccggacc
//
LOCUS       DATIIX010000074         3263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_18154, whole genome shotgun sequence.
ACCESSION   DATIIX010000074 DATIIX010000000
VERSION     DATIIX010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18154"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /locus_tag="VLF64_02185"
     CDS             <1..1386
                     /locus_tag="VLF64_02185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HSW90783.1"
                     /translation="QGPLNITLALLLAFIFQPVKNFFDKLTNKIFYSSNFTIGDFVVR
                     LSKKLTMSNDLRTLLHHAALDIADVLKIDQAFFIVRYGEGTSMSAGTREHKHVSQSDE
                     RTLDEYFYSNQEEILLTDTLLSDEGSIKDALNQHSIAAVLPLWYAGHLQGYLFLGEKH
                     SGYYVNRDLKALRTIRDELIIAIQNSLSVQEVREINATLQQRIKDATKKLRQSNNQLQ
                     RLDAAKDEFVSMASHQLRTPLTSVKGYISMVLEGDVGKITDEQRQLLEEAFTSSERMV
                     HLIGDFLNVSRLQTGKFIIDAHPVDLAKVAKQEVESIRQIAATHGMTVTYSSPSRFPE
                     LYIDENKIRQVIMNFMDNAIYYSPDSQEIKVMLSIEEGDAVLRVIDKGMGVPEDAQKK
                     LFTKFFRAENARRQRPDGTGIGLYLAHKVINGHGGRLVFESKEGEGSTFGFRLPIKKL
                     STPLPLQQETA"
     gene            complement(1469..3142)
                     /locus_tag="VLF64_02190"
     CDS             complement(1469..3142)
                     /locus_tag="VLF64_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90784.1"
                     /translation="MTLTSLLGKVSKRIWALTAIATATVAIPEVLLAWVPDRPTFTMA
                     HPSDHVTFNSITDNPVQGDERNFVQIRNVTTNSKFSENESLVAGNEYEVYVFYHNDAA
                     SNLNDAAHNYKGIATGAYMKTELPASVAAGSTGTINGYVGASNSSPSQVWDEATVKNT
                     TGGAMDIRIESGSAKITNNGATNGKTLPDAIFSTGTPLGYDTLDGKVPGCTQYSGYVT
                     YRFKADQPNFTVAKTVSTHNADNFSKTVSAKAGDLVDFLVQYKNTGTTEQDGVVIRDT
                     LPTNLSYVAGSTYLSNSLTGNKFQAITANTVAQQGINIGNYIPGAGGFVKLTAQVAAD
                     SALACGTNTLTNTAAADTQNGSKSDTATVTVTKDCVEQVQACNLTTFKIETVDKTKID
                     NVHYTTDLTKCQPVEQVQACNLATMKVEMVDKSKIDNVHYTTDLTKCAPKPETVVACN
                     LDTFKIETVDKTKIDDVHYSTDLTKCQEQVCRISDKTIVTINKSDFDNSKYTTDLSQC
                     ATTPTPTPTPPTTTELPHTGISDGVMSVIGAGSLVGMVGAYWGSRRITK"
BASE COUNT          838 a          713 c          788 g          924 t
ORIGIN      
        1 caggggccgc ttaatattac acttgcgctc ttacttgcct ttatttttca acctgtaaag
       61 aatttctttg ataaattaac aaacaaaata ttctacagta gcaacttcac gattggtgat
      121 tttgttgtac gactaagtaa aaaactgacg atgtctaatg acttacgaac actactccat
      181 catgctgcac ttgatatcgc cgatgtcctt aaaatagacc aagctttctt tatcgtacgg
      241 tatggagagg gtacttcaat gtccgctggg acgcgtgagc ataagcatgt gtcgcaatca
      301 gacgagcgta cgcttgatga atatttttat agcaatcagg aagagatctt gctaacggac
      361 acacttctct cagacgaagg tagcattaaa gacgcgttga atcagcatag tatagccgcc
      421 gtcctgccgc tctggtatgc tgggcatctt caagggtatc ttttcttggg cgaaaaacat
      481 tcaggttact atgttaatcg tgaccttaag gcgttgagaa ccattcgaga cgaactcatt
      541 atcgctattc aaaattccct gtccgttcaa gaagtgcgcg agattaacgc tacgctacaa
      601 caacgcatta aggacgccac aaagaagctt cgtcagtcca acaaccagct tcagcgcttg
      661 gatgcagcca aagatgagtt tgtaagtatg gcttctcatc agcttcgtac accgctcaca
      721 agtgtaaaag gatatatatc tatggttctt gaaggggatg ttggaaaaat cacagatgag
      781 caacgccaac tgcttgaaga ggcatttaca agcagcgagc gcatggtcca tctaattggt
      841 gattttctta atgttagtcg tctgcaaact ggtaaattca tcatcgatgc ccatcctgtc
      901 gatcttgcga aggttgctaa gcaggaagtg gaaagcattc gtcagatcgc cgcgacccat
      961 ggcatgacgg ttacgtatag cagtccatcg cggtttccag agctatatat tgatgaaaat
     1021 aagattcgtc aggtaatcat gaattttatg gataatgcta tttactactc acctgatagc
     1081 caggaaatta aggtaatgct ctcaatcgaa gagggcgatg ctgtgcttcg ggtaatagat
     1141 aaaggcatgg gagtgcctga ggacgcgcaa aagaagctct tcacgaaatt ctttagagcc
     1201 gagaatgctc gcaggcagcg tccagatgga acaggtatcg gtctctactt ggcccataaa
     1261 gttatcaatg gacatggtgg aaggctagtg tttgaatcaa aggaaggcga gggaagcaca
     1321 tttgggttcc gtttacctat caaaaagctc tcaacaccgc tgccgctgca gcaggagacc
     1381 gcataaacgc aaaagccacc catatcacag ggtggctttt cggtattaag gtgcgaaccc
     1441 taaacgcgag cggtcagctt agttttgctt atttagtaat gcgcctactc ccccaataag
     1501 ctcctaccat accaacgagc gatcccgctc caatcacact catcacacca tcactgatac
     1561 cagtgtgagg aagttcggtg gtggttggtg gagttggggt tggtgttggt gtcgttgcac
     1621 actgacttag gtcagtggtg tacttgctgt tgtcgaagtc agacttgtta atggtcacaa
     1681 tagtcttgtc gctaatgcgg cagacttgtt cctgacactt cgtaaggtca gtactgtagt
     1741 gaacgtcatc aatcttggtc ttatcgacag tctcgatctt gaacgtatca aggttacaag
     1801 caacaaccgt ttcaggcttt ggtgcacact ttgtaagatc agttgtatag tgaacgttgt
     1861 cgatcttcga cttgtctacc atctcaactt tcatcgtagc aaggttacat gcttgtacct
     1921 gttctactgg ttgacacttg gtaaggtcag ttgtgtagtg gacattatct attttagtct
     1981 tatccacagt ctcaatcttg aacgtggtga ggttgcaggc ttggacttgt tcgacacagt
     2041 ccttagtaac ggtaacagtt gcagtgtcgc ttttgctacc attttgggtg tctgcggctg
     2101 ccgtgtttgt caatgtgttt gtaccacaag caagtgcgct atcggcggct acttgggcgg
     2161 tgagcttgac aaatccaccc gctccaggaa tatagttacc aatattgata ccttgctgag
     2221 caaccgtgtt tgcagtaatt gcttggaact tgttaccggt cagtgagttt gagaggtatg
     2281 tagaaccagc tacgtagcta aggttagtag gaagtgtatc ccggataacg acgccatctt
     2341 gctctgtagt accagtattt ttgtactgga cgaggaagtc aacgaggtcg ccagcctttg
     2401 cgcttactgt tttactaaag ttgtcagcat tatgtgtgct tactgtttta gcaacagtga
     2461 agtttggctg gtctgcctta aagcgatagg taacgtagcc tgagtactgc gtacagccag
     2521 gtactttacc gtcaagtgtg tcgtagccaa gtggtgtacc ggtgctaaaa atagcgtccg
     2581 gaagggtctt gccattagtc gcgccattgt tggtaatctt agcactgcca gattcgatac
     2641 gaatatccat cgcacctcca gtagtgtttt ttaccgttgc ctcatcccaa acttggcttg
     2701 gtgatgagtt gctcgcgcca acgtagccgt tgattgtgcc agttgagccg gccgcgacgc
     2761 ttgctggaag ctcagtcttc atataggcgc ctgttgcgat acccttgtag ttatgcgcgg
     2821 catcgttaag gttgcttgct gcatcattgt gataaaatac gtaaacttcg tactcgttac
     2881 cagcgacaag tgattcattc tcactaaact tgctgtttgt tgtaacgttg cggatctgaa
     2941 cgaaattacg ttcgtcaccc tgtaccggat tatcggtgat tgagttgaat gttacgtggt
     3001 cggatggatg agccatcgtg aacgttggac ggtctggtac ccatgcgaga agtacttcag
     3061 gaatcgcaac tgtagcagta gcgatcgctg taagagccca tatacgcttc gagaccttac
     3121 cgagtagtga tgttagggtc atttcgctat ctccttagac gcgcctatag cgcgttatta
     3181 aaattggtgg tgtactacga caaaaatccg agaacactcg gaataagcaa aactaattgt
     3241 caatgtactt caccatcccg aga
//
LOCUS       DATIIX010000075         2904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_519761, whole genome shotgun sequence.
ACCESSION   DATIIX010000075 DATIIX010000000
VERSION     DATIIX010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519761"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VLF64_02195"
     CDS             <1..191
                     /locus_tag="VLF64_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HSW90785.1"
                     /translation="DDKELPRNAVLLFGAEGPGIREELLTHADEIVAIEQYGSTRSIN
                     VGVAAGIVMYEWVRIHKR"
     gene            248..1597
                     /gene="hisS"
                     /locus_tag="VLF64_02200"
     CDS             248..1597
                     /gene="hisS"
                     /locus_tag="VLF64_02200"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HSW90786.1"
                     /translation="MANLSSEPYKGARDWYPESMRLRSYIFHTWRKVAQRYGYEAYDT
                     PLLEPVDVYEAKSGQELVGEQTYQFTDRGERRVAVRPEMTPSVSRMIAARQQELAIPA
                     RWFSIAQFMRYERPQRGREREFWQLNCDLFGQEGAIADAEIISLGADILTELGATPEM
                     FTIKINNRRIVNFMMAQYLELDGVQAQLMIRLFDRKNKIAPEAFRDQAIEIFGTNMAS
                     KGLQKLAQLLAAKTMADLPETIRESKAVKEIQELFTQLERAGVKNAIFDITLMRGLDY
                     YTGTVFEFFDTHPENARALFGGGRYDGLVGLFGGEPMSGVGIAPGLSMTELFMHTHRL
                     VPKLRSNTDVYMIVIGDTLRSAQKLAIELRKEGVNIELDTTGRKMDKQLKTAVKKSIP
                     YLLFVGEEEIAKEAYSLKNTLTGEEKKLSFERIVSTVTDHRTGHSEDDEFDVSDMIA"
     gene            1622..2215
                     /locus_tag="VLF64_02205"
     CDS             1622..2215
                     /locus_tag="VLF64_02205"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSW90787.1"
                     /translation="MKHRVRRGFTIVELIIVIVVIAILATLTALLYSNAQRQARDTAI
                     RDAADKFAQSIELMSSKINGGAIIKGGYGSTTAATSTGCIDGSGGEQAPGYGAQKSDA
                     NYKCTLGDAVVAAGYLSASYFTGLPPNVADGNSPVTNFITQKCSGDGKNYLFYSQEAP
                     SSDSLAAVNNVLSTVCASQVSSVSGRNMGGVIQLDVR"
     gene            2244..>2904
                     /gene="tig"
                     /locus_tag="VLF64_02210"
     CDS             2244..>2904
                     /gene="tig"
                     /locus_tag="VLF64_02210"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HSW90788.1"
                     /translation="MKTTVKHLSDTRVLVTITLGATELEAAEQVALKKLAKNVKVDGF
                     RKGHVPIALIKKNVDPNELGQETIENALSRAVAESYLEKDIQALERPEVEIKKFVPGE
                     SLEFTAEGDVMPKVTLGDYKNLKAEKKVKAVVKADIEDTIERIRKGFATKEETKSAAK
                     DSDEVIIKFVGKKDGKAFDGGTGDDYPLTLGSKSFIPGFEEALVGLKAGDKKEVELTF
                     PE"
BASE COUNT          833 a          668 c          732 g          671 t
ORIGIN      
        1 tagacgacaa agagctacca cgtaatgcag tgctgctttt tggcgctgaa gggcccggga
       61 ttcgtgaaga attactcact catgcagacg aaatcgtcgc tatcgagcaa tacggcagta
      121 cgcgcagcat taatgttgga gttgccgcag gtatcgttat gtacgaatgg gtgcgcattc
      181 acaagaggta agtgatgatg ataatgcttg caaaatgcgc ataatattgc tataatataa
      241 gtcagttatg gcaaatctgt cctcagaacc gtacaagggt gcccgcgact ggtatccaga
      301 gtcaatgcga cttcgcagct atatttttca tacctggcgc aaggtagcgc agcgctacgg
      361 atacgaggca tatgatacac ctcttctgga gccagttgat gtatacgagg caaaaagtgg
      421 ccaagaactg gttggcgaac agacctatca attcaccgat cgcggcgagc gccgcgtcgc
      481 agtccgcccc gaaatgacgc cgagtgtgag ccgtatgatc gccgcgcgcc agcaagagct
      541 cgctataccc gcaaggtggt ttagtattgc gcagttcatg cggtatgagc gaccccagcg
      601 cggtcgcgaa cgcgagttct ggcagcttaa ctgcgaccta ttcggccaag agggtgcaat
      661 cgccgacgcc gagattatct cacttggtgc tgatatcctc acagaacttg gcgctactcc
      721 agaaatgttt acaatcaaaa tcaacaaccg ccgtatcgta aactttatga tggcacagta
      781 ccttgagctt gatggtgtgc aagcacagct catgatccgc ctttttgacc gcaagaacaa
      841 aattgcgcca gaagcattcc gcgaccaagc aattgaaatt ttcggcacaa acatggcatc
      901 taaaggacta cagaaactcg cccaactcct cgctgcaaaa accatggcag acttacctga
      961 aacaattcgt gagagcaagg ctgtaaaaga aatccaagaa ttgttcaccc agctcgaacg
     1021 cgccggcgta aagaacgcca tatttgatat caccctaatg cgcggccttg actactatac
     1081 cggtaccgta ttcgagttct tcgacacgca ccccgaaaac gcccgcgcgc tctttggcgg
     1141 cggtcgctac gatgggcttg ttgggctatt tggcggcgaa ccaatgagtg gcgttggcat
     1201 agcgccaggt ctcagtatga ccgagttatt catgcacact cacaggctcg taccaaagct
     1261 ccgcagcaac actgatgtat atatgattgt cattggcgat acactccgta gcgctcagaa
     1321 acttgccata gagcttcgta aggaaggtgt caatatagag cttgatacga caggccgtaa
     1381 aatggataag caacttaaaa ctgctgtcaa gaaatcgatt ccttatctat tgtttgtcgg
     1441 cgaagaagag atcgccaaag aggcatactc actcaaaaac acgcttaccg gtgaagaaaa
     1501 gaagcttagt tttgagcgaa tcgtcagcac cgtcaccgat caccgcactg gccatagtga
     1561 ggacgacgaa tttgacgttt ctgatatgat tgcgtagtct tgcttatgtt atactaatgg
     1621 catgaagcat cgggtgaggc gtgggtttac cattgttgaa ttaatcattg tgattgtggt
     1681 catcgcaatc ctagcaacac ttacagcgct gttatacagc aatgcgcaaa ggcaagcgcg
     1741 cgatacggcg atccgtgatg cagcagacaa atttgctcaa tctattgaac tcatgtcatc
     1801 aaaaataaac gggggcgcca taataaaagg tggttacggg tcgacaaccg ctgcgacatc
     1861 aacgggctgc atcgacggaa gtggtggcga gcaagcacca ggatatggag cgcagaagag
     1921 tgatgcaaac tataagtgta cgttaggtga cgctgttgtc gcagctggct atttaagtgc
     1981 atcgtacttt acaggattgc cacccaatgt cgcagatggc aatagtccag taacgaattt
     2041 cattactcaa aaatgcagtg gtgatggcaa aaactatcta ttctatagcc aagaagcgcc
     2101 gtcatcggac agtttagccg ctgtaaataa tgtccttagt acagtttgcg caagccaagt
     2161 gtcaagcgtg agcggccgca acatgggtgg cgtgattcag ctcgatgtgc gttagatagt
     2221 cctatctgtt ataatctgta gttatgaaaa cgacagtcaa acacctatct gatacgcgtg
     2281 tgctagtaac gattacactt ggggctacag aacttgaagc tgccgagcaa gttgcgctga
     2341 aaaagctagc aaagaatgtt aaggttgatg gttttcgtaa gggccatgta ccaatcgcgt
     2401 tgatcaagaa aaacgttgat cctaatgagc ttggacaaga aactatcgag aacgctcttt
     2461 ctcgcgcggt tgcggaaagc tatctcgaaa aggacataca ggctcttgag cgtcccgaag
     2521 tcgaaatcaa gaaattcgta cctggagaat cactcgaatt tacagccgaa ggcgatgtca
     2581 tgcctaaagt aacactcggt gactacaaaa atctcaaggc tgagaaaaag gtgaaagccg
     2641 tcgtaaaggc cgatatcgaa gatacaatcg aacgcatccg caaaggtttc gcgactaaag
     2701 aagagaccaa atctgcagca aaagacagcg atgaggtaat catcaagttt gttggcaaaa
     2761 aagatggtaa agcgtttgat ggtggtactg gtgacgatta tccgctcaca cttggtagca
     2821 aatcgtttat tcccggcttt gaagaggcgc tcgttggcct taaggctggc gataaaaagg
     2881 aagttgaact taccttccct gagg
//
LOCUS       DATIIX010000076         4010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_407910, whole genome shotgun sequence.
ACCESSION   DATIIX010000076 DATIIX010000000
VERSION     DATIIX010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407910"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1485)
                     /locus_tag="VLF64_02215"
     CDS             complement(<1..1485)
                     /locus_tag="VLF64_02215"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HSW90789.1"
                     /translation="MRRFGVGESEPAYSFLQLTFPADTTKSAYATEQLHILLRSMVKY
                     YSFWDRLAARKQPYSLEIVATRDDGIRYIIRIPKRELETVKRTLLSFLPGLKVTEIDD
                     YLPDSASLPTSITELKLANDFVLPLGNHKALEEHDPIAYLTGHMRNLPTDASISFQIV
                     AVPVFNNTHPNELRHIRNVEHKIALSKQLRPKLTEYKINVPRILLRAVLAPFWLIAMG
                     AKIIAGVIEAITTSDLPSDWKGGPKRSSDNPYEQELGNTIKGKLDQQLFEVSMRVLVA
                     SPDSSDNHTRLNAIVSSFDTFSSTYQHMTTRKVAPLITSVDKWVARFHNRSLLCHVVT
                     QQTILSSSELSDLYHFPNTDLTKTEGLVKSRSRELAAPLSIKRSDAKLDVIVGANLFG
                     GEVQEIGMTLEQRQKHTYIIGKTGTGKTTLLKSSIYQDMLNGKGLAVLDPHGDMFREL
                     LSIVPENRRKDVVVFDPSDRDYPVGLNILDPGIEFASEDDKHEWI"
     gene            complement(1749..1985)
                     /locus_tag="VLF64_02220"
     CDS             complement(1749..1985)
                     /locus_tag="VLF64_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSW90790.1"
                     /translation="MTRDAKESKYLSAFGKRLAEVRRAKGFTQESLAEKADVTALTVS
                     YIEQGRQWPRITTLHKLAKSLGVPVTELFRDLKS"
     gene            2101..2541
                     /locus_tag="VLF64_02225"
     CDS             2101..2541
                     /locus_tag="VLF64_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90791.1"
                     /translation="MKVHGLFIEKYSLNHKPLSQQLAEHIATHGLQGKVAVVADKPIT
                     LLASTRKYWFRLIRLTENERASTLNAARKEYLEVRLERMRQLQFTAKPPEDLLEADIT
                     FATADDFVRNPPDCKMIYITYSFERKKLHMLASWMPRNGVVVVL"
     gene            2721..3845
                     /locus_tag="VLF64_02230"
     CDS             2721..3845
                     /locus_tag="VLF64_02230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSW90792.1"
                     /translation="MTQELSRIAIVCDFLTTMGGAENVVLAMHEAFPDAPIYTAIYNA
                     DKMPAFSKLDIRTSSLQNMPKKLRNYHKLFPTLAVKAMRKLDLSEFDIILTSSYLHGH
                     QVTKSRPDQVIINYCHTPARYYWSHYDEYRRDPGYGKLNPIIRTLMPLMIPHQRKLDL
                     EAAKQVDVFIANSSETAKRIKKYYGRNATIIHPPVDVMRFSPSRERNDYYVTIGRQLP
                     YKRYDLTVQAATQLGINLKVFGNGPMHEKLVAMAGPTVHFYTDRFGDASNSEVEEALN
                     NAKGFIYPAEEDFGIVTVEALAAGAPAIALAKAGTLDIVQDGETGILFEHQTVEDVVT
                     AIKRAEEITFFPSKLQRTAKRFNTSLFITKLRKVVTDQIH"
BASE COUNT         1072 a          923 c          991 g         1024 t
ORIGIN      
        1 gatccactcg tgcttgtcgt cttcactcgc aaattcaatc cctggatcga ggatgttgag
       61 gccaacggga tagtcacgat cagacggatc gaacacgacc acatccttgc ggcgattttc
      121 cggcacgata gagagcagtt cgcggaacat gtcaccgtgc ggatcgagta cggccaagcc
      181 tttgccattg agcatatctt ggtaaatgga gctcttgagc agtgttgttt taccggtgcc
      241 ggtcttacca ataatgtagg tgtgcttttg gcgttgttcc aaagtcatac caatttcctg
      301 cacttcaccg ccaaaaagat ttgcaccaac gatgacgtcg agcttcgcat cgctgcgctt
      361 aattgaaagt ggcgcagcca actcgcgact acggctcttt acgaggcctt cagtcttggt
      421 aaggtcagtg tttgggaaat gatagaggtc ggacaactca gagctggata aaatggtctg
      481 ctgggtaacg acatggcaaa gcagcgaacg attgtgaaac cgggcgaccc atttatctac
      541 ggaggtaatg agtggtgcta ctttacgtgt agtcatgtgc tgatacgtgc ttgaaaaggt
      601 atcaaatgac gaaacgatgg cgttgaggcg tgtgtggttg tcgctgctat ccggggaggc
      661 gacaaggacg cgcatagata cctcaaatag ctgctgatcg agcttccctt tgattgtatt
      721 acctagttcc tgctcgtatg ggttatccga ggatcgtttg gggccgcctt tccagtcact
      781 cggaagatca cttgtcgtaa tagcctcaat cacaccagca ataatctttg cgcccatcgc
      841 gataagccag aatggtgcaa gtacggcacg aagtaatatg cgtggtacgt tgatcttata
      901 ttcggtcaat ttagggcgga gctgcttact gagtgctatt ttatgttcaa cattgcgtat
      961 gtggcgtagc tcgttgggat gagtattgtt aaagaccgga acggcgacaa tctggaaaga
     1021 gatcgacgcg tcagtcggca aattgcgcat gtgcccggta agatacgcaa ttgggtcatg
     1081 ctcctcaagt gctttatggt tgcctaacgg caatacaaaa tcatttgcaa gcttcagctc
     1141 agtgatgctt gtaggaaggc tcgctgagtc aggtaaatag tcatcaatct cagtaacttt
     1201 caggccgggc aagaatgaca gaagcgtgcg ttttacggtt tctaactctc gcttcggtat
     1261 tctgattatg tatcgaatgc catcatcacg agttgcgaca atctccaatg aataaggttg
     1321 tttacgtgcg gcgagccgat cccagaaact gtagtacttc accatgctgc gcaggagaat
     1381 atgcagctgc tcagtcgcat aagcggattt ggtagtatct gctggaaatg tgagttgtag
     1441 gaacgaatac gcgggctcac tctccccgac accaaagcgc cgcaatataa aggaaaagca
     1501 tgtacgagat agtattgtcc tagtttgatg agcagcagga taaggatata cgcagcgcca
     1561 aactcgagca gcaatgctcc gataacaacc actagtgtcc agccatcact caggtgatgc
     1621 gactgtgtgt agcgagctgg accgttgatg agggtgtcta tcaaatgctg catgtgtggt
     1681 ttctccagaa ttgcagttca cgacatccac accatgtccg ctgctagggt ttatacataa
     1741 ggtttgattt aacttttgag gtctctgaac aattcagtaa ccggaacgcc taaagatttt
     1801 gcgagtttgt gcaatgtcgt aatgcggggc cactggcggc cttgctcgat gtagcttacg
     1861 gtcaaggctg taacatctgc cttctcggct aatgattctt gcgtaaaccc tttggcacga
     1921 cgaacttctg cgagtcgctt gccgaaggct gataagtact tggattcttt tgcgtctcta
     1981 gtcataggtt cagcgtatat aactatgagt gttagtaaca cttagcacta ctaatagttg
     2041 agagttcgtg ttatgctcaa aaccagatgt aactattacc aatgctaata gttaccggag
     2101 atgaaagtgc acggtctatt tattgaaaaa tactcattaa atcacaaacc attatctcag
     2161 cagttggctg agcacatagc cacgcatgga ttacagggaa aggtggcggt tgttgcggat
     2221 aagccgatca cgctgcttgc atccactcgt aagtactggt tcagacttat ccgcctaact
     2281 gagaatgaaa gagcaagtac actcaacgcc gctcgaaaag aatatctcga agtacgccta
     2341 gaacggatgc gccaactaca gttcacggca aaaccgccag aagatttact ggaagcggac
     2401 ataacatttg caacagcaga tgattttgta cgcaatccgc ccgattgcaa gatgatctat
     2461 ataacctaca gctttgagcg caaaaaactc catatgctag caagttggat gccgagaaat
     2521 ggtgtagtgg tggttctata gataagacta agccgctcta tacaatcgag gacattctac
     2581 cgcaccttaa gtttattcgt actaagtggc aggagtatat tcttattggt aacatcaagt
     2641 atataattgc caattatata gagaggagtg atcaaacgta atgataagcg ttattactat
     2701 caatccgcta tactggtagt atgactcagg aactttcgcg cattgctatt gtctgtgatt
     2761 ttcttaccac catgggtggg gcggaaaatg ttgtgctggc aatgcacgag gcgttccccg
     2821 atgcgccgat ttataccgct atttataacg cggataaaat gccggcattt agtaagctgg
     2881 atatccgtac atcgagcctg caaaacatgc cgaagaaact acgcaactac cacaagctgt
     2941 tccctacgct tgccgttaag gctatgcgca agcttgacct gagtgagttc gatattatcc
     3001 tgacgagcag ctacctgcac ggccaccagg tgacgaaatc tcgcccagac caagtgatta
     3061 ttaattactg ccacacacca gcacgctact actggagcca ttacgatgaa taccgccgcg
     3121 acccaggata cggcaaactc aaccctatta tccgcacatt gatgccactg atgatcccgc
     3181 accagcgcaa gcttgactta gaggctgcta aacaagtgga tgtgtttatt gccaattcgt
     3241 ctgagaccgc taagcgtatt aagaagtact acggccgaaa tgctactatt attcatccgc
     3301 cggtcgatgt gatgcggttt agtccatcgc gcgagcgtaa cgactactat gtaaccattg
     3361 ggcgccaact gccatacaaa cgctatgatc tgactgtgca agcggccacg caacttggta
     3421 ttaatctgaa ggtatttggc aatggcccga tgcacgaaaa actcgtcgcg atggctgggc
     3481 caactgtgca tttttacacg gatcgatttg gcgatgcatc gaatagtgaa gttgaagagg
     3541 cacttaataa tgctaaagga ttcatttacc cagccgagga agatttcggg attgttactg
     3601 tggaggcact cgccgctggt gcgcctgcga tcgcacttgc aaaggctggc acgctcgata
     3661 tcgtgcagga cggtgaaaca ggcatactgt ttgagcatca aacggtcgag gatgtggtta
     3721 ccgcgatcaa gcgagccgag gaaattacat tcttcccgtc gaaattacag cgcacggcaa
     3781 aacgatttaa tacgtcactg tttattacta agctgcgcaa ggttgttacc gaccaaattc
     3841 actagcacaa cagctcgcca taatgctata gtttacttat ggagagttta ccggtggggc
     3901 aaaatgatgt tgagaggctt aatgccgagc ctgctacagg tccaggaaca ccaatagctc
     3961 aggatactcc acctcagaat atccaaccgc agatggctcc cccatctgcg
//
LOCUS       DATIIX010000077         5798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_520152, whole genome shotgun sequence.
ACCESSION   DATIIX010000077 DATIIX010000000
VERSION     DATIIX010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520152"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1064)
                     /locus_tag="VLF64_02235"
     CDS             complement(87..1064)
                     /locus_tag="VLF64_02235"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HSW90793.1"
                     /translation="MSKEKNTKAFAQAKQHRRQWITFLRMCRYGVNNFTRNTWLTIAA
                     TAVMTLTLVTVLITVAAQNILTDTATHIARNIDRSIYLKTGTTEKQAGPIIGDLKKLS
                     NVESVNFISTEQGKADFAQKNKSDLKTLSALTQATDKIPATIRVNLKNVNDTSQLVDF
                     VRTNKPLKQYIDDSTPPSFMGNRKSATDTIAAWTHTAQQVGIGASILFTAMSMLIIFN
                     TIRMAIFNRRDEIEMMKLIGADKGFIRGPFVVEAMVYGFIAAVIATVLTFMIMLSIHD
                     KVAAWGVSIQPTMELYTTYIGLVLLGAIVVGALIGTISSLLATRRYLKI"
     gene            complement(1061..1744)
                     /gene="ftsE"
                     /locus_tag="VLF64_02240"
     CDS             complement(1061..1744)
                     /gene="ftsE"
                     /locus_tag="VLF64_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HSW90794.1"
                     /translation="MILLDRVTKSYGKDGKPALNRVSLHVEPNEFVIIVGTSGAGKST
                     LLKLLTREEKPTSGKIVVGGIDYDTLKDKHIPLLRRKIGVVFQDFKLLPQRTVFENVA
                     FALEIAGMTNREIKNTVPKVIELVGLTGKEKQFPHQLSGGERQRVAIARAVVRQPKIL
                     IADEPTGNLDPKHSWDIVRLLEKINKYGTTVLLTTHNVEIVNKLKRRVITIEHGKITS
                     DQAQGSYRQ"
     gene            complement(1800..2885)
                     /gene="prfB"
                     /locus_tag="VLF64_02245"
     CDS             complement(1800..2885)
                     /gene="prfB"
                     /locus_tag="VLF64_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HSW90795.1"
                     /translation="MQPLKKRANELLADITADCDSLHISAKSEELEAIDAQLADANVW
                     ANLERAQNLSRQSATLKGQVEPWNILHSQVSDIVELMELGDDSMAAEFNSQLDVFELE
                     YAQRHKELLLSGEYDSHAAVLKLSSGAGGTDAQDWTEMLERMYLRWAEANDMHVELIE
                     RSTGEEAGIKNATYIVSGSYAYGRLRSEHGVHRLVRLSPFNSDNLRQTSFALVEIMPE
                     IDAPDEVKVDDKDLKIDVYRSGGHGGQSVNTTDSAVRVTHIPTGIVVAIQNERSQIQN
                     KETALKILRSKLAQMLAEQHADTLSDLRAGESASWGAQIRNYVLHPYTLVKDTRTKYE
                     VTDAKSVLDGNIDGFIDAYLTAQATKQ"
     gene            2934..3581
                     /locus_tag="VLF64_02250"
     CDS             2934..3581
                     /locus_tag="VLF64_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HSW90796.1"
                     /translation="MKIGVFDSGRGGEFIAEGLQRLLPEHTFIVVNDREHVPYGSRKD
                     SEIIELTTKAIQPLLEVHCPIIVIACNTATMAAIAILRRQFPEIKFVGIEPMIKPAAT
                     TSTTHHATVLATPLTLKSKRYQHLLHQYASELIIDQPNAAGWAAAIEHDKADMISFDE
                     VATSIASGSDTVILACTHYITLKRQLQIRFPGVNILEPTEAIARQITRLAFEFEM"
     gene            complement(3550..4437)
                     /locus_tag="VLF64_02255"
     CDS             complement(3550..4437)
                     /locus_tag="VLF64_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HSW90797.1"
                     /translation="MAKDVTKGRLAVQRAFSTSDIVVLAITFVVFFLASSTYFIINYF
                     TNDMSGSDTADIIMVGIIGTIIGAGVLFGVALVVGMFMVRLQRQVMLGNSLQVEYSAY
                     AWLRDWANEVSADLEMPRVEIFVTQDPVINAYSFGFARPYAIVLQSGTIRYLSKDELK
                     VVLVHEMAHIKYGHVNAAVYLQPFMAVPVLGAIFTWLAGFWQRRTEFTADRLALMYMQ
                     DSELVKQALIKVHVGPDVASSMNDIARQWLQYTAERPMNHLAQTFSNHPFLVRRISRI
                     DERKHIVEPVTSQTQKPVA"
     gene            complement(4439..5716)
                     /gene="murD"
                     /locus_tag="VLF64_02260"
     CDS             complement(4439..5716)
                     /gene="murD"
                     /locus_tag="VLF64_02260"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HSW90798.1"
                     /translation="MKVAIAGYGIEGKASFDYWCAKGAEVTIADERDSLSVPAEAAGA
                     ILGAEAFAELGDFDLIIRSPSINPVKLPYGDKVWSATNEFLKECPAPIIGVTGTKGKG
                     TTSSLIAAILKESGCTVHLVGNIGVPALEELASIKKDDIVVYEMSSFQLWDAHASPRI
                     AIILMLEPDHLDVHTSFEEYIEAKSQIVRHQSADDIALYHPTNRWSSEIAHSGVSQPV
                     RYGIPDDGQVYVDGDAFYAEDTRICSVDALQLVGEFNRENACAAISAALAAGAERSAV
                     EAGLKSFHGLNHRLKFVRTVDGVSYYDDSIATTSGSAIAALRSFAAPRFIILGGSSKG
                     VEYDEIVAECRAANAAVIAIGQTGETIAHLCAEQEVVCYRMTGRMNEVVEKAHEVAPE
                     GSVVILSPASASFDQYDNYSHRGDQFVAAVEAL"
BASE COUNT         1601 a         1529 c         1154 g         1514 t
ORIGIN      
        1 cataagcata aaccgtatgt caagttgttt gtgcgtcata aaactcccct ctctccatat
       61 cacctcatta ggaggtagac gggcgattaa atcttcaggt agcgcctagt tgcaagtagt
      121 gatgaaattg taccaataag cgctccgacc acgatagcac caagcaacac aagtccaatg
      181 tacgtagtat agagttccat tgtaggttgg atagacactc cccatgcggc aaccttatca
      241 tgaatactca gcataatcat aaatgttaat acggttgcga taaccgctgc aataaagcca
      301 tataccatag cttcaaccac aaatggaccg cgaataaagc ctttatctgc gccaattaac
      361 ttcatcatct caatctcatc acgacggtta aagatggcca tgcgaatcgt attaaagata
      421 atcagcattg acatcgcggt aaagagaatg cttgcaccaa tacctacctg ttgcgcagta
      481 tgtgtccatg ctgcaatcgt atcggttgct gacttgcggt tgcccataaa acttggcggt
      541 gtcgaatcat caatatattg cttgaggggt ttgttggtcc tcacaaaatc cacaagctgg
      601 ctcgtatcat tcacgttttt aagattaacg cgaattgtcg ctggaatttt atcagtggcc
      661 tgagtaagtg cactcagggt tttaagatca ctcttattct tctgggcaaa atccgccttc
      721 ccctgctcgg tactaataaa gttcaccgac tcgacattcg aaagtttctt aagatcacct
      781 ataatcggac ctgcctgctt ttcagtcgtc cctgtcttga gataaataga gcgatcgata
      841 ttacgagcaa tatgcgttgc cgtatcagta aggatgttct gggcagcaac agtaatgagc
      901 accgttacta gcgttagggt cattacagca gttgctgcga ttgttagcca ggtattacga
      961 gtaaagttat ttacgccata gcgacacatt cgaaggaatg taatccactg acgacgatgc
     1021 tgctttgcct gcgcaaaagc cttagtgttc ttttccttac tcattgtcgg tagctcccct
     1081 gagcttggtc agacgtaatc ttgccatgct caatcgtaat cacgcggcgt ttcaacttgt
     1141 taacgatctc gacattgtga gtagttaaaa ggacagtagt gccgtattta ttaatctttt
     1201 ccagtaatcg aacaatatcc cagctgtgct ttgggtcaag gttacccgtt ggctcatcgg
     1261 caattaaaat cttcggttgg cgtacaactg cacgcgcgat tgccacgcgc tgacgctccc
     1321 caccggaaag ttggtgtggg aactgcttct ctttgccagt taagcccaca agctcaatta
     1381 cctttggtac tgtattctta atctcacggt tagtcatacc ggcaatttcg agtgcaaatg
     1441 ctacgttctc aaataccgta cgctgtggca ataatttgaa atcctgaaaa accacaccaa
     1501 ttttacgacg aagtagtggg atatgtttat ctttcagtgt gtcgtaatca ataccgccaa
     1561 cgacgatctt accactagtt ggcttctctt cacgtgtcaa caatttaagt agcgtgctct
     1621 tacctgctcc agacgtaccg acaataatca caaattcatt cggctcgaca tgcaaagaca
     1681 cccggtttaa tgccggcttg ccatctttgc cgtacgactt ggttacccta tctaacagaa
     1741 tcattactta gcccagtata gcataagaat aaccactaaa ccaccgtgcc atgagcacgt
     1801 tactgcttag ttgcttgggc ggtaagatat gcatcaataa atccatcaat attaccatcg
     1861 agcaccgatt ttgcatccgt aacttcgtat tttgtacgcg tatctttaac gagtgtgtaa
     1921 gggtgcaata cgtaattgcg aatctgcgcg ccccaacttg cagactcccc tgcacgtaga
     1981 tccgatagcg tatcagcgtg ctgttcggcg agcatctgtg caagtttgct acgaagaatc
     2041 ttaagtgctg tttctttatt ttgtatctga ctgcgctcat tctgaatagc cacaacgatc
     2101 ccagtcggaa tatgagtgac acgcactgca ctatcagtag tattcacact ctgtccacca
     2161 tggccgccgc tccggtatac atcaattttg agatctttat cgtccacttt cacttcatca
     2221 ggcgcatcga tttctggcat aatttcaaca agagcaaaac ttgtctgacg gaggttatcc
     2281 gagttaaacg gactcaagcg aaccaagcga tgtacgccat gctcactacg aagccgacca
     2341 taagcgtaag acccgctcac gatataagta gcatttttaa tacccgcctc ttcgccagta
     2401 gaacgctcga taagctcaac atgcatatcg tttgcctctg cccagcgcag atacatacgc
     2461 tcaagcatct ccgtccaatc ctgcgcatca gtaccgccag cgcccgaact taacttcagc
     2521 acggccgcat gggaatcata ttcaccactc agtagtaatt ccttatggcg ctgagcatac
     2581 tccaactcga atacatcaag ctgactatta aattccgctg ccatgctatc gtcacccagc
     2641 tccatcaatt caacaatatc gctcacctgc gaatgcagaa tattccacgg ctcaacttgg
     2701 cctttaagtg tggctgattg gcgcgataaa ttctgcgcac gctctaaatt ggcccaaaca
     2761 tttgcatcgg caagttgcgc atcgattgct tctaattctt cagactttgc gctgatatga
     2821 agtgagtcgc agtcggcggt gatatcggca agtaattcgt ttgcccgttt cttcagtggt
     2881 tgcatctctt tacattgtat catcacctct agcgttagtg atacactgag catatgaaga
     2941 ttggcgtatt tgattctggc cgcggcggtg aattcatcgc agagggtttg caaagactct
     3001 taccagaaca tacgtttata gtagtaaacg atcgcgaaca cgtaccctat ggatcacgca
     3061 aagatagtga gattattgag cttacaacaa aggctattca gccgctacta gaggttcact
     3121 gccctattat cgtcattgct tgcaataccg caacaatggc tgcaattgct atcttacgga
     3181 gacaatttcc agaaataaaa tttgttggca tcgaaccaat gattaagccg gcagccacca
     3241 catcaactac ccatcacgcg actgtacttg caaccccgct tacactcaaa agtaagcgat
     3301 atcagcatct actccatcaa tacgccagtg aacttattat tgaccagcca aatgcagctg
     3361 ggtgggccgc cgctattgaa cacgacaagg ctgacatgat ttcatttgat gaagtggcta
     3421 caagtatagc aagtggcagt gatacagtca tccttgcctg cacacattac attacactca
     3481 agcgtcagct acaaattcgc ttccctggcg tgaatattct agagcccaca gaggcaattg
     3541 ctcgtcaaat tacgcgactg gcttttgagt ttgagatgtg accggctcaa cgatatgctt
     3601 tcgttcgtca attctactaa tacgacgtac taaaaaggga tggttgctga acgtctgcgc
     3661 aagatggttc atcggtcgct cagcggtata ttgaagccac tggcgagcaa tatcattcat
     3721 actactcgca acgtcagggc caacatgcac cttaataagc gcttgcttca caagttccga
     3781 atcttgcata tacataagcg cgagccgatc agccgtaaac tcagtgcgac gctgccaaaa
     3841 gccggcaagc caggtaaaaa tagcaccaag tacaggcacg gccataaatg gttgtaagta
     3901 caccgcagca ttcacatggc catatttaat gtgtgccatc tcatgcacta acaccacctt
     3961 gagctcatct ttgcttaagt agcgaatcgt acctgattgc aacacgatag cgtacggacg
     4021 cgcaaatcca aatgaatagg cattaatcac cggatcctgt gtcacaaata tctccacccg
     4081 tggcatttcc aaatccgccg acacttcatt agcccaatca cgaagccacg catacgcgct
     4141 atattcaacc tgcagtgaat tacccagcat tacttggcgc tgcagtcgga ccataaacat
     4201 accaactaca agtgccacac caaaaagtac acccgcacca atgatagtac cgataatccc
     4261 caccataatt atatctgccg tgtcgctacc agacatatca ttcgtaaagt agttaataat
     4321 aaaatatgta cttgatgcta agaagaaaac aacaaacgta atggcaagta cgacaatgtc
     4381 actcgtcgaa aaagcccgct ggaccgccaa ccggcctttt gtcacatcct ttgccatctt
     4441 atagcgcctc tactgcggcc acgaattggt cgccacgatg actataatta tcatactgat
     4501 caaaactcgc actcgcagga ctcagtataa cgacactccc ctcgggcgca acttcatgcg
     4561 ccttttcaac cacttcgttc atgcgcccag tcatgcggta gcacactact tcttgctcag
     4621 cacaaagatg tgcgattgtt tcaccagtct ggccaatagc aatcaccgca gcatttgctg
     4681 ctctacattc agcaacaatc tcatcgtact ctacaccctt gctcgagcca cccaaaataa
     4741 taaaccgagg tgccgcaaat gatcgtaatg ccgcaatagc actccccgac gtagtagcaa
     4801 tactatcatc gtaataggat acgccatcca ctgtgcgtac aaacttcaat cgatggttaa
     4861 gaccatggaa tgatttcagg ccggcctcaa ccgcagatcg ctcggcccca gctgcaagcg
     4921 ccgcactaat agcagcacac gcattctcac ggttaaactc accaaccaac tgtagcgcat
     4981 caaccgagca aatacgcgta tcttcagcat agaatgcatc accatctaca tacacttgcc
     5041 catcatcagg gataccgtat cgaacaggct gtgacacgcc agaatgtgca atttcacttg
     5101 accaccgatt tgttggatga tacagtgcaa tgtcatcagc actctgatga cgaacaatct
     5161 gcgactttgc ttcaatatac tcctcaaagc tcgtgtgtac atctagatga tccggctcaa
     5221 gcatcaatat aatcgcaata cgtggactcg cgtgggcatc ccacagctga aaactcgaca
     5281 tttcgtaaac aacgatgtca tctttcttaa tgctcgcaag ctcctctagt gccggcacgc
     5341 caatattacc caccaggtga accgtacagc ctgattcttt caaaatggcc gctattagac
     5401 ttgaggtcgt acccttacct ttggtgccag tcacaccaat gattggtgcg gggcactctt
     5461 tcaaaaattc attagttgcc gaccaaactt tatccccgta aggtaacttt acgggattga
     5521 tacttggcga acggataatc aaatcaaaat ctccaagttc agcaaacgcc tcagcaccta
     5581 aaatcgcccc agcagcctca gcaggcacgc ttaggctatc gcgctcatct gcaatcgtta
     5641 cttcggcgcc tttggcgcac caataatcaa agctcgcctt cccctcaatt ccgtagccag
     5701 cgattgcaac cttcatatgt atattgtacc agttttagga ttcaggagtt gagaaaacct
     5761 taccaagctt gtcgctcagc gttacgatat cctcttta
//
LOCUS       DATIIX010000078         7372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_352735, whole genome shotgun sequence.
ACCESSION   DATIIX010000078 DATIIX010000000
VERSION     DATIIX010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7372
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352735"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1738
                     /gene="uvrA"
                     /locus_tag="VLF64_02265"
     CDS             <1..1738
                     /gene="uvrA"
                     /locus_tag="VLF64_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HSW90799.1"
                     /translation="QGGGRYYDSTYEGVIPNLERRHTETDSEFMRKDIERFMRERECT
                     ACHGARLKPVVLAVTVHGLSIMDICNLGVDDAIELFNGLKFDEGEMTIAKLVLKEIMA
                     RLGFMSNVGLSYLELSRAANTLSGGEAQRIRLATQIGSGLQGVLYVLDEPSIGLHQRD
                     NDKLIETLKHLRDLGNSVLVVEHDEDTIAQADFLVDVGPGAGVHGGMIVAAGSPSEVA
                     KNPDSITGRYLVGTEKIAVPKKRRAIDRDRKLLVRGAKENNLKNIDAAFPLGVMTLVT
                     GVSGSGKSTLVNDILAKELAARLHRAQEVPGTHDNIEGINQLDKAIIIDQSAIGRTPR
                     SNPATYTGVFTPIRELFAGTPEANIRGYKAGRFSFNVKGGRCENCQGDGVIKIEMHFL
                     PDVYIQCSECHGKRYNREALEIKYKGKTISDILEMTVAEAAEFFENVPAIYRKLATIN
                     EVGLGYIKLGQPATTFSGGEAQRIKLASELSRRSTGKTLYILDEPTTGLHTADVKKLL
                     EILQRLVEGGNSMVIIEHNLEVIKCADWIIDLGPEGGQGGGMIIAEGTPEDIAKAKGS
                     FTGKYLKDVLAK"
     gene            complement(1768..2409)
                     /locus_tag="VLF64_02270"
     CDS             complement(1768..2409)
                     /locus_tag="VLF64_02270"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HSW90800.1"
                     /translation="MENLLHAITNLGPLAVILVIFAESGLMAGFFLPGDSLLFIAGTL
                     VNQGIFHINIVIFAALLFGAAVVGNSTGYYIGRKVGRRLFRRPDSRFFRQEYLQQAEN
                     FYEDNGSKAIVMAMFVPIVRAFSPVVAGISRMPYKTFVTFNLIGALIWTCGFTFLGYF
                     AGAVIQKLGINIEIAALLIIFLSLLPGLAHVLKEPETRAKITHHIKKRITRRK"
     gene            2519..3229
                     /locus_tag="VLF64_02275"
     CDS             2519..3229
                     /locus_tag="VLF64_02275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HSW90801.1"
                     /translation="MGEKISLSISERTVHGKKVRSLRAKGITPGVVYGHGIEPMPIQA
                     DTGEVRRVVAQAGKHTPVHLTGAKRRIALIKDIEFDATKHGIVRHVSFHAVNADEPVH
                     AEVPVHLLGIGESEAEKAGFVVLQAIDKAEVRALPADLPEALEADITTLATVGDRVTL
                     GDIKLPSGVELVEHDTGRHDDDEEEEKPSITDLVVANVYEPAALEAANEAAAGEGINE
                     EQVAVEGEQETGAETKES"
     gene            complement(3276..3983)
                     /locus_tag="VLF64_02280"
     CDS             complement(3276..3983)
                     /locus_tag="VLF64_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HSW90802.1"
                     /translation="MTYEPDVHTVQTSILRALLFVPHANFAELQKTTSLSSDHFTFHI
                     KKLIGAGLVEKMDSGYTLTPHGKEYANRMDTDENEIEKQPKVSVALIVERQQGNRREF
                     IVQQRLKQPYYGFYGRLGGKVRWGESFAEAAARELKEETGLIGKFTFSHIFHKRDYRK
                     LDGSLLEDKIFVIMYCNNVSGEMMEAFEGGRNFWMTQEELIAQDKIFESARDFVKLYD
                     QGIHYHAQEYTYDDSEY"
     gene            complement(4031..5788)
                     /locus_tag="VLF64_02285"
     CDS             complement(4031..5788)
                     /locus_tag="VLF64_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSW90803.1"
                     /translation="MQKDILALFWRTTKPFSKRRNLAIFFVLFSVITEGYVAPFILSK
                     FIDMLQAGSITLNGSIPLIAAYGGTLLASTVISWRLTLWFTWSFEVLAARNLASKVIK
                     HLLGHSLNFHANRFGGSIISQTTKLLSSFERFWDTIIWDLLPIFASITAAVTILSFVY
                     WQYALFLLVVSVIFALSVLFGSRFMRTLNERETEAWNKMTGFIADVITNIATVKAFGG
                     STHEQGSMDARTAEWGDRSFDSMRGFLKVSTVWSSLIMVIYVCALGFAAVAGEHHSIQ
                     VGAVYLMLTYTLGVGRNLWSLNHVMRQYNSVMGDALAMTKELKLEFDIKDKTTKHLDA
                     TKGEIAFDDVTFAHDGGKGERIFNNLSLTINPGERVGLVGHSGSGKTTLTRLLLRFSD
                     IDKGLITIDSQNIAEVTQQSLHQSIAYVAQEPLLFHRTLADNIAYGKPDATREQIIQA
                     AKQAHAWEFIQKLPEGLETMVGERGIKLSGGQRQRIAIARALLKDAPILVLDEATSAL
                     DSESEKLIQESLNTLMQKRTSIVIAHRLSTIAQLDRIIVLEHGTIAEDGSHQELLKKK
                     GIYASLWKHQSGGFIEADE"
     gene            complement(5989..>7372)
                     /locus_tag="VLF64_02290"
     CDS             complement(5989..>7372)
                     /locus_tag="VLF64_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSW90804.1"
                     /translation="IDFINGHVGIQDLFLLRTMSFAINILVGLTLIAAHTPTLAIVIL
                     FLIVGLLVQVRVFRRMREGIRAERKRLISEVNGAVADTITNNLTVKTFAGEAYEIGRT
                     EAISREYQRAYYKDFTIMSYEGSGRIFFMQIFQIASIVIIGTLLLHHAMPLAIAIFTI
                     AYLQRLAAQLFTLGELINGYDKILLEATPMTEILLDPPKVVDMSSTDLEVTNGVIDFD
                     NVSYAYSDSKGTDVLANLDLHIPAGQKVGLVGVSGAGKTTITKLLLRFDDLTNGVISI
                     DGQDIANITQHSLRSSIAYVAQEPLLFHRTLAENIAYGKPGATMDAIRDAAAEAHALE
                     FIERLPHQFETVVGERGIKLSGGQRQRVAIARAILKDAPILMLDEATSALDSESEKLI
                     QASLETLMEGRTSIVIAHRLSTIAKLDRIIVLDRGTIIEDGTHAELLKKKQGIYASLW
                     KHQSGGFIED"
BASE COUNT         2015 a         1703 c         1834 g         1820 t
ORIGIN      
        1 acagggcggc ggccgttact atgattcaac ctacgaaggt gtaattccaa acctcgagcg
       61 acgccatacg gaaaccgata gcgaatttat gcgtaaagac atcgagcgct ttatgcgtga
      121 gcgtgagtgt acggcatgtc atggcgctcg tttgaagcct gtagtactcg ctgtaacagt
      181 gcatggttta tcaatcatgg acatctgtaa tcttggtgtc gacgatgcaa tcgagctctt
      241 taatggcttg aagttcgacg aaggcgaaat gacgattgcg aaactcgtgc taaaggagat
      301 tatggcccgc cttggcttta tgagtaatgt cggcctcagc tatcttgagc tctcacgcgc
      361 agcgaacaca ttgagcggtg gtgaagcgca gcgtatccgt cttgcgaccc agatcggatc
      421 tggtctacaa ggtgtgctat atgtgctcga cgaaccatca atcggccttc atcagcgtga
      481 taatgacaaa ttgattgaga cattgaagca cctccgtgac cttggtaact cggtgttggt
      541 cgttgaacat gacgaagata ctattgcaca agcggatttc ttagttgatg taggtcctgg
      601 cgctggtgta cacggcggta tgattgttgc tgccggtagc ccgagtgaag ttgcaaagaa
      661 ccccgacagc attaccggtc gttacttagt aggaaccgaa aaaatcgccg taccgaaaaa
      721 acgtcgggca attgatcgcg atcgtaaact tttggtccgt ggtgccaaag aaaacaacct
      781 taagaacatc gacgccgcgt ttccacttgg cgtaatgacg cttgtaacgg gtgtgagcgg
      841 tagcggcaag tcaacgttag tgaatgatat cttagcgaaa gaacttgccg ctcgcttgca
      901 ccgtgcccaa gaagtgccag gcacacatga taatatcgag ggcattaacc agctcgataa
      961 agcaatcatt atcgaccagt cggcgattgg ccgtacgccg cgatctaacc cagcaacgta
     1021 caccggtgtg tttacgccaa tccgtgaact attcgctggc acgccagaag caaacatccg
     1081 cggctacaaa gcggggcgct ttagctttaa tgtaaagggt gggcgctgcg agaattgcca
     1141 gggtgatggg gtaattaaga ttgaaatgca cttcttgcct gatgtgtata tccaatgttc
     1201 tgaatgtcat ggtaagcgct ataaccgtga agcactagaa attaagtaca agggcaaaac
     1261 aatcagtgac atacttgaaa tgacggttgc agaggctgct gaattcttcg agaatgttcc
     1321 cgcgatctac cgtaaactcg caaccatcaa cgaagtaggg cttggctaca ttaaacttgg
     1381 tcagcccgca actaccttca gtggtggtga agcgcagcgt atcaaactcg cgagtgaatt
     1441 gagtcgacga agtaccggta agacgcttta catcctcgat gagccaacta cgggattaca
     1501 taccgccgac gtaaagaagc tgcttgagat cttgcaacgc cttgtggaag gtggtaacag
     1561 tatggtaatc atcgagcaca acctcgaggt gatcaaatgt gccgactgga tcattgacct
     1621 cggcccagaa ggcggtcagg gcggcggtat gattattgcc gagggcacgc ctgaggatat
     1681 cgcaaaagcc aagggcagct ttacgggtaa gtatctcaaa gacgtcctcg caaagtaaat
     1741 agtacaatct aaaagaatta ctcgccgtta tttgcggcga gtaattcttt ttttaatatg
     1801 gtgcgtaatc tttgctctgg tttcaggctc ttttaacaca tgagccagcc caggcagaag
     1861 tgataagaaa ataatgagca gggcagcgat ttcaatgttg ataccgagct tttgaatgac
     1921 ggctccggca aagtatccaa ggaacgtaaa gccgcatgtc catatgagtg ctcctattag
     1981 gttaaatgta acgaatgtct tatagggcat acgactgatc ccggcgacga caggcgagaa
     2041 ggcgcgaaca attggcacga acattgccat tacaatcgcc ttagaaccat tgtcttcgta
     2101 gaaattctca gcctgctgga ggtactcttg gcggaagaat cgactgtcgg ggcgacggaa
     2161 aagacgcctg cctactttgc gaccaatgta gtagccagta ctgttgccaa caactgctgc
     2221 cccgaagagc aatgcggcga atataacaat attgatatga aaaatgcctt ggttgacgag
     2281 tgtcccggca atgaaaagca ggctgtcacc gggcaagaag aaacctgcca tgagtcctga
     2341 ttcagcaaaa ataactaaga taacagcaag cgggcctaga ttggtaattg cgtgtaataa
     2401 gttctccatt actttgattg tagcatacta ctgtttgctt gagcattttg atacgtggta
     2461 tagtagtaag tgcaaatatc acacctccgc acaggtatct aacgaaagga agagagaaat
     2521 gggagagaag ataagtctta gtattagtga gcgtaccgtt cacggtaaga aagttcgaag
     2581 tctacgcgcc aagggcatta caccgggcgt tgtgtatggt cacggtatag aacctatgcc
     2641 aattcaagct gatactggcg aggttcggcg agttgtagcg caggcgggca aacatacgcc
     2701 ggtacaccta acaggggcaa aacgacgtat tgcattgatt aaagatattg agtttgacgc
     2761 cacaaaacat ggtatagttc gtcatgtttc gttccacgcg gtaaacgcag atgagccagt
     2821 acacgccgag gtcccggttc acttgcttgg tatcggtgaa agcgaagctg aaaaagcagg
     2881 atttgtcgtt ttgcaggcaa tagataaagc cgaagtcaga gcacttccag cagacttacc
     2941 cgaagcgctt gaagcagata ttaccactct tgcgactgtg ggtgatcgtg tcacgctcgg
     3001 tgacattaag ctgccaagcg gcgtagagct cgtcgagcac gacaccggtc gtcatgacga
     3061 tgatgaagag gaagaaaagc cttcaatcac cgacttagtc gttgcgaacg tgtacgagcc
     3121 cgctgcgctt gaggcggcta acgaagcggc cgcaggtgag ggcattaatg aagagcaagt
     3181 ggctgtagaa ggcgaacaag aaacaggtgc cgaaacgaag gaatcgtagg cagatacatc
     3241 aaaaataccg caccgtatat acggtgcggt attttttagt actcgctatc gtcatacgta
     3301 tattcttggg catgatagtg tataccctga tcatataact tgacgaaatc gcgggcgctt
     3361 tcgaagattt tatcctgcgc gataagctcc tcttgcgtca tccagaagtt ccttcctccc
     3421 tcaaatgctt ccatcatctc accacttaca ttgttacagt acataatgac gaagatttta
     3481 tcttcaagta aactgccgtc caatttacga taatcgcgtt tatggaagat atggctgaaa
     3541 gtgaatttgc caataagccc tgtctcttct ttcagctcgc gggcagctgc ttctgcaaaa
     3601 ctttcacccc atcgaacctt gccgccaagg cgaccataga atccatagta tggttgctta
     3661 aggcgttgct gtacaataaa ctcgcggcga ttgccctgct gtcgttccac aatcaatgcg
     3721 accgatactt ttggctgctt ctcgatctca ttttcgtcag tatccatgcg attagcatac
     3781 tctttaccat gcggcgtaag cgtgtagccg ctatccattt tttcaaccag ccccgcacca
     3841 atcagcttct tgatatgaaa tgtaaagtgg tcgctgctga ggctagttgt tttttgaagt
     3901 tcggcaaaat tagcatgagg cacgaacaaa agtgcgcgaa gtatgctagt ttgaacagtg
     3961 tgaacgtctg gttcgtaagt catagagcta tggtagcact ggctggccgg gcaatctagt
     4021 aaagtcgact ttactcatct gcctcgatga agccgcccga ctggtgcttc caaagcgaag
     4081 cgtagatgcc tttctttttg agcagttcct ggtgacttcc gtcctcagca attgtaccgt
     4141 gctcaagcac gataatacga tcaagttgcg caatggtact gagacggtga gcgattacaa
     4201 tactagtacg cttctgcatt aaggtattaa gtgattcttg aataagcttc tcactttcgc
     4261 tatcgagtgc tgacgtcgct tcatcaagca cgagaatggg tgcgtctttc aataatgcgc
     4321 gggcaatcgc tatgcgttgg cgttgacctc cactcaactt gatgccacgc tcaccaacca
     4381 ttgtctcaag gccttctgga agtttttgaa taaattccca tgcatgcgcc tgcttagccg
     4441 cttgaataat ttgctcgcgg gttgcatctg gcttgccata ggcgatatta tctgccagtg
     4501 tacggtggaa gagcaacggt tcctgtgcaa cataggcaat cgactggtgc aggctttgtt
     4561 gggtaacctc ggcaatattc tggctatcaa tggtgattaa gcctttgtca atgtcactga
     4621 atcgaagcag gagtcgggta agggttgttt tacctgagcc actatggcca acaaggccaa
     4681 cgcgttcacc cgggttaata gtgagtgaca aattattaaa aatccgctca cccttgccgc
     4741 cgtcatgggc gaatgtcaca tcatcgaatg caatctcacc ttttgtagca tcgaggtgct
     4801 tggtcgtttt gtctttaata tcaaactcga gctttagttc ttttgtcatc gctaacgcgt
     4861 cacccatcac gctattgtac tgacgcatta catggttgag tgaccaaagg ttccgaccaa
     4921 caccaagtgt gtaagtaagc atcaagtaaa cagctcccac ctgaatgctg tggtgctcac
     4981 ctgcaacagc ggcaaagccc agcgcacaaa cgtaaattac catgataagg ctcgaccata
     5041 cggtactaac tttcaagaat ccacgcatag aatcaaacga acgatcgccc cattccgcag
     5101 tgcgtgcgtc cattgagcct tgctcatggg ttgacccacc gaatgccttc accgttgcaa
     5161 tgtttgtaat aacatcagca atgaatccgg tcatcttgtt ccaggcttcg gtttcacgct
     5221 cgttcaacgt acgcataaaa cgtgagccaa ataagacgct aagcgcgaaa atgacagaaa
     5281 caaccaagag aaacagcgca tattgccaat agacaaacga aagaattgtt acggctgcag
     5341 taatgcttgc aaagattgga agcaagtccc agataatagt atcccagaaa cgctcgaacg
     5401 agctaagcaa cttagtggtc tgactgatga tcgaaccacc aaatcggttg gcgtggaaat
     5461 taaggctatg gcccaacaag tgcttgatga cttttgaagc taggttacgt gccgcaagta
     5521 cctcaaatga ccaggtaaac caaagcgtga ggcgccatga tatcacggta cttgcgagta
     5581 gcgtaccacc ataagcagcg attagtggaa tactaccgtt gagggtaatt gaaccagcct
     5641 gaagcatatc gataaacttc gacaggatga acggcgcgac atagccttcg gtgataaccg
     5701 aaaagagcac gaagaaaatt gcgagattgc gtcgctttga aaatggtttt gttgtgcgcc
     5761 agaatagcgc gagtatgtct ttttgcatac gatcctttct gggaatgggc acacctgtag
     5821 cgcgggtgct gatgatgaaa tgggagatta ctatgtagaa gtattacgcc acacggaagg
     5881 gcgcaataac ggcaattaca gaatctaaca ttgattggcc tttcctttag cggttaacaa
     5941 taaaagctat attaacacga tgttgttata tgtgcaagct ctgaagactt aatcttcgat
     6001 aaaacccccc gactgatgtt tccagagaga agcatagata ccctgtttct tcttgaggag
     6061 ttcagcgtga gtaccatctt caatgatggt accgcgatcg aggacaataa tgcgatcaag
     6121 ctttgcgata gtactcagac ggtgagcaat cacaatactc gtgcgacctt ccatgagtgt
     6181 ctcaaggctc gcctgaatga gcttctcact ttcactatcg agtgccgacg tcgcttcatc
     6241 aagcattagg attggtgcgt cctttaagat agcgcgggcg attgcgacgc gttggcgctg
     6301 gccaccgcta agcttaatgc cacgctcacc aacaactgtt tcaaattgat ggggcaatcg
     6361 ctcaatgaat tctaaggcat gcgcctctgc cgcggcatcg cggattgcat ccattgttgc
     6421 gcctggcttg ccataggcaa tgttctctgc aagcgttcgg tggaagagta gcggctcctg
     6481 agcgacataa gcgattgatg aacgcaagct atgctgagta atatttgcaa tatcctggcc
     6541 atcaatacta atcacgccat tggttaaatc gtcaaaacgc agcagcaact ttgtgattgt
     6601 cgttttgccc gcgcccgaca cgccgacgag accgaccttt tgcccagcag gaatatgcaa
     6661 gtcaagattc gcgagaacgt ctgttccctt ggagtcgcta tatgcataac tgacattatc
     6721 aaagtcgatc acaccattag taacctcaag atccgtgctt gacatgtcaa caacttttgg
     6781 cggatcaagc aaaatttctg tcatcggtgt tgcctcgagt aggattttat cgtagccatt
     6841 aataagctcg ccaagggtaa agagttgggc ggcgagacgc tgcaaatacg caatggtgaa
     6901 gatagcgatc gcaaggggca tggcatgatg aagtaggagt gtgccaatga ttacaatcga
     6961 agcaatctgg aagatctgca taaagaagat gcgcccggag ccctcgtagc tcataatcgt
     7021 gaaatctttg taatatgcgc gctgatactc gcggctaatt gcttctgtgc gcccgatctc
     7081 gtaggcttca ccagcgaatg ttttaacagt aaggttattc gtaatagtat ccgcaacggc
     7141 gccgtttacc tcggaaatta accgtttgcg ttcggcgcga ataccctcac gcatacgacg
     7201 gaacacccgt acctggacaa gtaggcccac gatcaagaag agtataacta tcgcgagcgt
     7261 tggcgtgtgc gctgcaataa gtgtaaggcc aacgaggata ttgatagcaa acgacattgt
     7321 gcgcagtaag aagagatctt gaataccaac atgaccgttg ataaaatcga ta
//
LOCUS       DATIIX010000079         8302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_352901, whole genome shotgun sequence.
ACCESSION   DATIIX010000079 DATIIX010000000
VERSION     DATIIX010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 8302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8302
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352901"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /gene="heR"
                     /locus_tag="VLF64_02295"
     CDS             <1..402
                     /gene="heR"
                     /locus_tag="VLF64_02295"
                     /inference="COORDINATES: protein motif:HMM:NF037099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HSW90805.1"
                     /translation="VGVYDLSSLVMIFALAAMMNLMGLVMEVHNQTTKKTSWLSYVMG
                     CLAGIVPWLVIAFYMWLGAENGSKAPTFVYWIFVSIFVFFNCFAINMFLQYKKIGPWK
                     NYVYGEYAYIILSLVAKSLLAWQVFAGTLRP"
     gene            477..1034
                     /locus_tag="VLF64_02300"
     CDS             477..1034
                     /locus_tag="VLF64_02300"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSW90806.1"
                     /translation="MKRVLRYDSRSLGFTIVELLIVIVIITILAAITIISYNGIQNRA
                     KDAALNDQIQKLQNAVELYNTANGSYPKSANWGQMGLADSTGVWPVQNMAQYGVTAQL
                     LVSPYDTSGEANSIQEQGNWTTSSRSLNGIIGWVSIIDPSLDASAGHWTCFNPGEICT
                     GYRFWWRTSDGVLHTIAGGLTPPSP"
     gene            1078..1347
                     /locus_tag="VLF64_02305"
     CDS             1078..1347
                     /locus_tag="VLF64_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90807.1"
                     /translation="MKSKRINLQSTLIEAAGWYGAAAVLLAYTLVSFNVISPHGWTYQ
                     LLNFTGAVGVLIISYMKHAKQPALLNFVWAIIAFVALVQLAINHI"
     gene            1449..2735
                     /gene="eno"
                     /locus_tag="VLF64_02310"
     CDS             1449..2735
                     /gene="eno"
                     /locus_tag="VLF64_02310"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HSW90808.1"
                     /translation="MKISSIHARQILDSRGNPTVEADVILENGILGRAAVPSGASTGS
                     HEAIELRDGGNLYGGKSVLHAVAHVNGEISEALVGMEVTNQTDIDQKMIDLDGTDNKG
                     RLGANAILAVSLACAKAAALAKDKPLYVYIASLAGNGKLMLPLPMMNIINGGKHAADS
                     TDIQEFMIMPVGAANFSDCIRMGAEIFQALGKVLRSKGYATTVGDEGGYAPAVKNGNA
                     EALDLISEAVASAGYKLGEDVVLALDVASSELMQNGKYSLATEGKRLSSEEMVTFYED
                     LANRFPIVSIEDGLGEDDWDGWKQLTKKLGNHIQLVGDDLLVTNTEFLKRGITEKAGN
                     AILIKVNQIGTLTETIRAVNMAHDAGWKAIISHRSGETEDTTIAHLAIGLGTGQIKTG
                     SLSRTDRVAKYNELMRIEEQLGEGATFAGRGAVEQK"
     gene            2735..3358
                     /locus_tag="VLF64_02315"
     CDS             2735..3358
                     /locus_tag="VLF64_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HSW90809.1"
                     /translation="MAYLVLVRHGQSKWNALGLWTGQRDIELTDQGREEARRAIEHLK
                     DIPLHKAHTSKLIRAKQTLEEIKQALNQVELETVEHEALNERDYGDYTAKNKWEIADQ
                     LGEEEFTKLRRNWDHPVPGGETLKDVHARVLPYYEQEILKDLKEGKNVIVAAHGNSLR
                     ALMKHLDSQPEDKVHELDIGTGEVLVYEISEDGTVLVKEIRAKGGKA"
     gene            3419..3991
                     /locus_tag="VLF64_02320"
     CDS             3419..3991
                     /locus_tag="VLF64_02320"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HSW90810.1"
                     /translation="MKSSPETLFFKTSDEWETWLAQNYTLEEGVLLKFAKKESGITSL
                     NYAGALDIALCYGWIDGQVYAIDETFYAQRFTPRRTRSIWSKRNVSKVEELIKLGKMK
                     VPGIAAVEAAKADGRWEQAYESSSNATIPPELQVALKANPAAKEFFETLNKTNRYSII
                     WRIATARKPETRAARITKLIAMLENHEKFH"
     gene            4031..4366
                     /locus_tag="VLF64_02325"
     CDS             4031..4366
                     /locus_tag="VLF64_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90811.1"
                     /translation="MTRLHRHKQQKSQKTLIVKKQQKPKFIDRLVYVAAIVEPLCSLP
                     QAYAVWSTRSAGSISILAWLGFVVMALIWIWYAIVNHERIIFIYQAMFFVIDSSVLIG
                     AIYFGGRLY"
     gene            5022..5873
                     /locus_tag="VLF64_02330"
     CDS             5022..5873
                     /locus_tag="VLF64_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90812.1"
                     /translation="MALKEGAHPQINDTHAALDHTIDKKDPPDRIIFEGMSAGGKEAL
                     DLAAYLREKYPKIDISIILSSSPYDQDSAYQLRGSNNVLPIIADLSAELNLRGGPITR
                     FLGEMYNKRHRCWDDDIFFRQCYETAKQVAHSKLTSDASSTELLEWQVEWTRSLSAGR
                     NINRLKNEDGKGYTSILYIKTTRDTVVDDAQAIEKYEADAAKNGIPFEVISIDAPHAS
                     EQANASLYNKKVIKTYLAEIDNIYAQIDTQNLADKALENKIGPSPSPSVAAIAPSPSN
                     IPDMAAK"
     gene            5918..7561
                     /locus_tag="VLF64_02335"
     CDS             5918..7561
                     /locus_tag="VLF64_02335"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HSW90813.1"
                     /translation="MSRKISEFVRSDDPGSYQPQSTPQLAVHPQRPRSVVEQKLSPAP
                     PAAAPSESLPSVDLESLDTHLEPLPEKKQRKRRFHIKFTKKRVILSIIVLLLLIAGGI
                     FTYLNYTASKVTNGGTILDLVAPGTPLKTDDEGRTNILVFGTSQDDTAHQNADGGGGL
                     WLTDSIMLVSINKSANTAKMVSIPRDLWVKLDDQCSVGNESKINAVYECAAGLINDAQ
                     SPSEYTKKDKEGAAALMKTVTTVTGITPQYYAHVNYNVLKQAVDAVGGVSVNIKGDGA
                     SGIYDTNFDWNCPNGRPYTCKNVYYPKDGTYELDGQHALYLARARADSGAYSYKDFGL
                     ARGDFDRQLNQQKIMTAIKEKAQSAGVLSNPVAVFNLLNALGNNITTSISTGEMKTFL
                     TTLQKLKSADITSVDLNGDTPVVKTGSYSGQSIVLSTSGLYDYTSMITLIAKQLSTNP
                     AIAEGASIAIYNGGETAGAAGTLQTKLTSGGLTVTATGNTDPTSSSASYTIYVAKPDS
                     FTKTVDYLTKNLASATVVQTAAPASVPANDADIVIVINK"
     gene            7561..>8302
                     /locus_tag="VLF64_02340"
     CDS             7561..>8302
                     /locus_tag="VLF64_02340"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSW90814.1"
                     /translation="MKHSCQILGTTTSYWEYNPDKPTTLIFIHGFRGTHHGLLKIADL
                     LPEYRLIIPDLPGFGVSEALTNGHGIDNYIEWLFLFRQIVDPQAPSSYIVGHSFGSII
                     VSHYASLHPLTVTKLTLINPIGAPALEGEQRFLTQLAILYYWLGRKLPARAARAWISF
                     KPITKITSVTMRRTKDRQIRKFIDEQHYTHFSTFANPTQLSEAFKTSVSHDVSQVAKH
                     LTMPTLMIVGEKDDITALSKQHELHAKID"
BASE COUNT         2451 a         2124 c         1834 g         1893 t
ORIGIN      
        1 gtgggcgtat acgacttgtc aagtttggta atgatttttg cattggccgc catgatgaac
       61 ttaatggggc ttgttatgga agtccacaat caaacaacaa aaaagacaag ctggctaagt
      121 tacgtcatgg gttgtttggc cggcatagta ccatggttgg tgatagcgtt ttatatgtgg
      181 cttggagccg aaaatggcag caaggctccg acgttcgtct actggatatt cgtctcgata
      241 ttcgtattct ttaactgttt tgcgataaat atgtttttac aatacaaaaa aattggaccc
      301 tggaaaaact acgtatatgg tgaatatgcc tatattattc tgagtctcgt tgccaaatcg
      361 ctattagcat ggcaagtctt tgctggaaca ctacgaccat agttcagtcg tctgtctaac
      421 cggtcaagct aaatcttttt caaaaggagt aaaggatgcc gtataataag taacgaatga
      481 aacgggtatt acgctacgat tcaagatcat taggctttac aattgtcgag ctgctaatcg
      541 tcatcgttat aatcacaatt ttggctgcaa tcactattat ttcctacaat ggaatccaga
      601 atcgcgccaa agatgctgcg cttaacgatc agatccaaaa actgcaaaac gccgtcgaac
      661 tatataacac agcgaatgga agctacccta aatcggcaaa ctgggggcag atgggcctcg
      721 ccgattctac tggcgtctgg cctgtccaaa acatggcaca atatggtgta acagctcagc
      781 ttttggtttc gccgtatgac acgtccgggg aagctaactc aattcaagag caaggcaact
      841 ggacgacatc ctctcggtca ctcaacggca tcattggctg ggtttcaata attgaccctt
      901 cgctcgatgc atctgccgga cactggacat gcttcaatcc tggtgagatc tgcacaggat
      961 atcgtttctg gtggcggaca agcgacggtg tgcttcatac aatagcaggc ggactaaccc
     1021 caccgtcacc ataaaaattt cgccgcaaac gagaaacatc acgtaaatgg aagatcaatg
     1081 aaatctaagc gcatcaacct acaatcaacc cttatcgaag cagctggttg gtacggtgca
     1141 gctgcggtgt tgcttgcata cacactcgtc agcttcaacg ttatttcgcc gcatggatgg
     1201 acgtaccagt tactcaattt cactggcgcc gttggtgtgc taattatttc gtacatgaag
     1261 cacgctaaac aacccgcatt gctcaatttt gtttgggcta tcattgcctt tgtcgcatta
     1321 gttcaactag ctatcaatca tatctaagtc attctcggta gtatgtagtt gtacattcgt
     1381 catacgcata cgcatattgt atatcccacc gtgggcgagt agcataaaag actggaggta
     1441 cacatataat gaaaatttca tccattcacg cccgacagat tctagatagc cgcggcaacc
     1501 caactgttga agcagatgtt atactcgaga acggtatcct tggccgtgca gcggtgccat
     1561 caggtgccag cactggatcg catgaggcaa tcgaacttcg tgacggtggt aatttatatg
     1621 gcggcaaatc cgtattacat gctgttgcac atgttaatgg cgagatcagc gaagcacttg
     1681 ttggtatgga agtaactaac cagacagata tcgaccaaaa gatgattgac ctcgacggta
     1741 cggataacaa aggccgtcta ggggcaaacg caattttggc agtgtcactc gcatgcgcaa
     1801 aggcagcagc gctcgcgaaa gacaaaccat tatatgtata tattgcatcg cttgctggca
     1861 acggcaaact catgctgcca ctccctatga tgaacatcat taatggcggc aagcacgcag
     1921 cagatagcac tgacattcag gaatttatga ttatgcccgt tggtgctgca aattttagcg
     1981 actgcattcg tatgggcgct gagatttttc aagctcttgg caaagtactg aggagcaaag
     2041 gctacgcgac aaccgtcggc gacgaaggcg gatatgcgcc agcagtgaag aatggcaacg
     2101 ccgaagccct cgatcttatt agtgaagcag tggcaagcgc cggctacaaa cttggcgaag
     2161 atgtggtgct cgcgctcgac gtagcaagta gtgaacttat gcaaaacggt aaatatagcc
     2221 ttgcaactga aggcaaacgg ctttctagcg aagaaatggt tacattctat gaagatctcg
     2281 ctaacagatt cccgatcgta tcgatcgaag acggtctcgg cgaagatgat tgggatggct
     2341 ggaagcaact caccaaaaaa ctcggcaacc acattcagct cgttggtgac gatctgctcg
     2401 taaccaacac tgagttctta aagcgcggca ttaccgaaaa agctggtaac gcgattctga
     2461 tcaaagtaaa ccaaatcggc acgctcaccg aaacgatccg cgctgtcaat atggctcacg
     2521 atgcaggctg gaaggcgatt atttcacatc gctctggcga aacagaggat acgacaatcg
     2581 cacaccttgc aatcggcctc ggcactggcc aaattaaaac aggtagcctt tcacgaaccg
     2641 accgcgtagc aaaatataac gagctcatgc gcatcgaaga gcagctcggc gaaggcgcca
     2701 cctttgcagg tcgcggtgcc gtggagcaaa aataatggca tacctcgtat tagttcgcca
     2761 tggtcaatct aagtggaatg ccctcggact ctggacaggt cagcgcgata tcgagctcac
     2821 cgatcaaggc agggaagaag cacgtcgtgc cattgaacac ctaaaagaca ttcccctcca
     2881 caaagcacat acgtcaaagc taattcgcgc caagcaaaca ctcgaggaaa tcaagcaagc
     2941 actcaatcaa gttgagctcg aaacagttga gcatgaagcc ctcaatgagc gtgattatgg
     3001 cgactacact gccaaaaaca agtgggaaat cgccgaccaa ctcggcgagg aggaattcac
     3061 gaagcttcgc cgtaactggg atcatccagt gccaggcggc gaaacgctca aggatgttca
     3121 tgctcgcgta ttaccatact acgaacaaga aatcctcaaa gatctcaaag agggtaaaaa
     3181 tgtaattgta gccgcacatg gcaattcact ccgcgcgctc atgaaacatc ttgatagcca
     3241 gccagaagat aaagtgcatg aactcgatat cggcacaggt gaagtactgg tctatgagat
     3301 ttcagaagat ggcacagtcc tcgtcaaaga aatccgtgcc aaaggcggca aagcctaaca
     3361 attaatccga aaacaagcgc tccacccagg acgcttgttt tgatatagta atataagcat
     3421 gaaatctagt cccgaaacac tctttttcaa aacatccgac gaatgggaaa cctggcttgc
     3481 gcaaaactat acccttgaag agggcgtatt actcaaattc gctaaaaaag aatcgggcat
     3541 tacctcactg aattatgcag gtgcgctcga catcgcacta tgttatggct ggattgacgg
     3601 ccaagtctat gctatcgacg aaacgttcta tgcacaaaga tttacgccgc gccgcacccg
     3661 aagcatttgg tcgaagcgca acgtcagtaa ggtcgaggaa ctcataaagc tcggcaaaat
     3721 gaaagtccca ggcatcgccg cagtcgaggc ggccaaagca gacggtcgtt gggagcaggc
     3781 gtatgaatca tcgtcaaatg ccaccatacc gccggaacta caagtcgcac tcaaggcgaa
     3841 tccagccgct aaagaattct tcgaaaccct taataaaacc aatcgctact ccataatctg
     3901 gcgcattgcc acggcgcgta aacccgagac gcgtgctgct cgaattacca agcttattgc
     3961 tatgcttgaa aatcacgaaa agttccacta acattggaca tttctcgtat ggaagcttat
     4021 gctaggcata atgactcgct tacatcgaca caagcagcaa aaatcgcaaa aaacactcat
     4081 tgttaaaaag cagcaaaagc caaaatttat tgaccggctt gtctatgtcg ccgcaatcgt
     4141 cgaaccactc tgctcgctcc cacaggccta cgcagtctgg agcacgcgct cagcaggtag
     4201 tatctcaata ctcgcatggc tcggttttgt cgttatggcc ttaatttgga tttggtatgc
     4261 aatcgttaac cacgaacgta tcatctttat ttaccaggca atgttcttcg tcatcgatag
     4321 ttctgtactc atcggggcaa tctacttcgg cggccgatta tactaaatcg caaataaaat
     4381 gcccctcagt tgtgaggggc attttatttg cctttcgttc ttttgagctc atcagcatac
     4441 atatgcatgt atgcaggcag tcctttaacg cacacgtgtc attatgtaat tcttctagtg
     4501 agattattgc ccaagctaat gacagtaagg taccatatat acatgactga ggtactgcat
     4561 cgaccgccaa gcccagacca atcaccatgt tcgcaaaatg actacgaccg ccgcatgctt
     4621 gatgcgcaaa caattgaaag gcaagacaaa ccaaaaataa cttctatgtc accagtgtcc
     4681 ctgagcgaac tcctcacccg aaaagagcgt aaaaggcgtg atcgggaaga gcgacgcgca
     4741 agacctgggt atacacgccg tcgaattggc gtcgcagcgc tctgccttgc cgaatcactt
     4801 ggcctcacat acaaaggctt tatgttcgac caaaacactc tcgccaatat ggttggggca
     4861 gcgacaaggc ggaattccat acgctctacg aacatttcga tggcgatatc acccccgaaa
     4921 cctttgtata tgtcatcccg ggtaccggta ttaaagacgc tacgcctcgc atagccacac
     4981 cactcatgcc ctcattttca tcaataccaa atgtcaaata catggcatta aaagagggtg
     5041 cacatccaca aattaacgac actcacgctg cgctggatca cactatcgac aaaaaagacc
     5101 cgccagaccg tatcattttt gagggtatga gcgcaggcgg aaaagaagcg cttgatcttg
     5161 ctgcgtactt acgtgagaaa tacccgaaaa tcgatatttc tatcatcctt agcagctcgc
     5221 catatgacca agactccgcc tatcaattgc gtggatccaa taatgtgctg cccattattg
     5281 ccgacctctc tgcagaactc aatttgcgtg gcggtccgat aacacgcttc ctcggcgaga
     5341 tgtacaacaa acgccatcga tgctgggacg acgacatttt ctttagacaa tgctatgaaa
     5401 ctgccaagca agtcgcccat agcaaattaa cgtccgacgc ttcatcaact gaactactcg
     5461 aatggcaagt ggagtggaca cgctccctct ccgccggccg caatattaac aggctaaaaa
     5521 atgaagatgg gaaaggatac acatcaattc tgtatataaa gacgacaagg gatactgtcg
     5581 ttgacgatgc gcaagctata gaaaagtacg aagcagacgc cgccaaaaac ggtattccat
     5641 tcgaagtcat ctcaattgac gctccacatg ccagcgagca agctaacgca agcctctata
     5701 ataaaaaggt tatcaaaaca taccttgccg aaatcgacaa tatttacgca cagattgaca
     5761 cccaaaacct cgcggacaag gcgctagaga ataaaatcgg gccgagcccg tcaccatcag
     5821 tcgcagctat agccccaagt ccaagcaata tacctgacat ggctgctaag tagccataca
     5881 atgtattctt aagtctacgc gtgttaaaat aagtagcatg tctagaaaga tcagtgaatt
     5941 tgtgcggtca gatgaccctg gctcgtacca gcctcagtcg accccacaac tcgccgtaca
     6001 tccacaacgc ccacgctcag tggtagagca aaagctttcg cctgctccac ccgccgcagc
     6061 gccaagcgag tcgctacctt ccgttgattt agaatcgctc gatacgcatc tcgagccact
     6121 acccgaaaaa aagcagcgca aacggcgatt ccatattaag ttcacaaaaa aacgagtgat
     6181 cctctcaatt attgtactat tgttgctgat tgctggcggc atctttacat acttaaacta
     6241 caccgcctca aaagtcacaa acggcggcac aatccttgat cttgtcgccc caggaacccc
     6301 tcttaaaaca gatgacgaag gccgtacaaa tatcctcgta ttcggcacct cgcaggatga
     6361 taccgcacat caaaatgcag atggcggtgg tggtttatgg ctcaccgact caattatgct
     6421 cgtgagcatc aacaaaagcg ccaacacggc aaaaatggta agcatcccac gtgatttatg
     6481 ggtcaaactc gacgaccaat gctcggtagg aaatgaatca aagattaacg ccgtctacga
     6541 atgcgccgct ggactcataa acgacgcgca atcaccatca gagtatacta agaaagacaa
     6601 agaaggcgct gcagccttaa tgaagaccgt cacgacagtc actggtatca caccccaata
     6661 ttatgcacat gttaactaca atgtactcaa gcaagcagtg gatgcggtag gtggcgttag
     6721 cgttaatatt aagggtgacg gcgcaagcgg tatttatgac actaacttcg actggaactg
     6781 cccaaatggc cggccataca cctgcaaaaa cgtctactat ccgaaagacg gcacttatga
     6841 actcgatggt caacacgcac tatacctggc gcgcgcgcgt gccgatagtg gagcatattc
     6901 atataaagat ttcgggctcg ctcgcggcga tttcgatcgc cagctaaacc agcaaaagat
     6961 tatgacagct atcaaagaga aagcgcaatc tgcgggcgtg ctgtcaaatc cagttgccgt
     7021 ctttaatcta ctaaatgcgc ttggtaacaa tatcactaca agcatcagca ctggcgagat
     7081 gaagacattc ctcacaactc tgcaaaagct taaatccgcc gatatcacaa gtgttgacct
     7141 aaacggtgat acaccagttg taaagaccgg atcgtactca ggacaaagca tcgtactctc
     7201 gacgagcggc ctctatgact atactagtat gatcactctc atcgccaaac aactctcaac
     7261 taacccagcc atcgccgaag gtgctagcat agccatctat aatggcggcg agactgccgg
     7321 cgcagcgggt acactccaga cgaagctcac atcaggcgga cttactgtca cagcaactgg
     7381 caacaccgat ccaaccagct caagtgcgtc gtatacgatt tacgtggcaa aaccagattc
     7441 atttacaaaa actgtcgact atctcaccaa gaaccttgct agtgctactg ttgttcaaac
     7501 cgcagcgcca gctagtgttc ccgctaatga cgcagacatc gtcatcgtaa ttaataagta
     7561 atgaaacata gttgccaaat cctcggaacc actacaagct attgggaata caaccccgat
     7621 aaaccaacca cgctcatttt cattcacggg ttccgcggca cacatcacgg gctactcaaa
     7681 attgccgatc ttctgcctga atatcgatta atcattccgg atctcccagg ctttggtgtg
     7741 agcgaggcgc taacaaacgg tcatggaatc gacaactaca ttgaatggct attcctattc
     7801 cgccaaatcg tggatccaca ggcaccctcg tcatacatcg tcggacactc attcggttct
     7861 atcatcgtca gccattacgc aagccttcac ccgttaaccg ttacgaagct tacgctcatc
     7921 aatccaatcg gagcacccgc cctcgaaggc gaacaacgat ttcttacaca acttgctatt
     7981 ctttactact ggctcgggcg caagttgcca gcccgtgccg cacgagcctg gatttcgttc
     8041 aagccgataa ctaagattac aagtgtcacg atgcgtagaa ccaaagaccg ccagatacgc
     8101 aagtttatcg atgagcaaca ctatacacac ttcagtacat ttgcaaaccc gacgcagtta
     8161 tcagaggcat tcaagacatc tgtaagtcat gacgtcagtc aggttgccaa acatctcact
     8221 atgccgacac tcatgattgt aggcgaaaaa gacgacataa cagcgctcag caagcagcac
     8281 gagttacacg ctaaaatcga tg
//
LOCUS       DATIIX010000080         4629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_546887, whole genome shotgun sequence.
ACCESSION   DATIIX010000080 DATIIX010000000
VERSION     DATIIX010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546887"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..2772
                     /locus_tag="VLF64_02345"
     CDS             181..2772
                     /locus_tag="VLF64_02345"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HSW90815.1"
                     /translation="MSEQFPKSRSNTSRQEHGTSEIPGGYYRYQRSARQLQAEHTGSP
                     DIFLDKFKEQEYAQVISAAITAQKGIADLMKTNPKKAAIEQKRYEPLFTASAEAQRVL
                     TEANLRFAAYYARASMNIVAPKDDYPAKRRRGAFGDITRLRSPYADLEERTQVANEAL
                     VKAAQTFVPKQDEKGTPISFLGHAGYRIHKALSIHAKTEEAPGWHVSAGEVEKLYAAR
                     KDPTSVDALELERLDAMDEGRQTVPIDEFTLEVVAPEEADGFDDLHALSLADVLPDPN
                     GEPVDIEALLNLRDGRIRKVLDTLSEREAGVISMRFGLDDGTMKKLDEIAAVYGVTRE
                     RIRQIESKTMGKLRHQSRSRLLRGFVGLEAQDFDIVNPAVLPHKADGTQNIRTTYSVA
                     HQGGQSVETAEEAEFTHKASEHMASWQVFPDEPWDIQPANERVSQTFEVTAQHLERLL
                     YNTPPYTFQHSFAKEVASRYPIALVATINEQLGTSLTSSHIEDFWNHHINNYVASLRE
                     QLGDDFSLDRVSALFTRLLAEYMLDDDRVELHIPPELDGKLHGMGAELQTGELILYGN
                     VGDFTGHRMNGMASIMVNGSTGDFTAFESAGYASLEVMGDAGHFAGSRLTEAAYLHVH
                     GSVGDYCGQGLKGIDAYIEVDGNAGSHVGDNALRGVIDVRGVGESAQSNSSAVVNGEF
                     AEWEAKDTTRVPVSLGKLAVHPPYDTEVIKRVLTPQPKDPQATIAEMEAAAQSVNPSL
                     LEHAMPAPMQAELSPERLTFSQACEWFGVSAERLNECLEEIGHMRDADLTTSDMGVMS
                     ELYEDLQVETVPTEGFISTEKVIEKFKIRKTPTQLLRLLAKKNVPLYKFRDVDGCVKH
                     FISNENL"
     gene            3037..3294
                     /locus_tag="VLF64_02350"
     CDS             3037..3294
                     /locus_tag="VLF64_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90816.1"
                     /translation="MALLGCVMYALRQTRRPREVQASALLRCVTEGPEGYLIVQVSGN
                     TFLAPVSQRAMIESFKGSSWPMPFMLLAVEPIDGLRVGTLR"
     gene            3465..3848
                     /locus_tag="VLF64_02355"
     CDS             3465..3848
                     /locus_tag="VLF64_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90817.1"
                     /translation="MNIYISHCGGAYDYKTELYAPIKESELAKTHYFFLPHEPENLDT
                     DAVTELKRMDVLVAEASFPSTGQGIELAQAKAANVSIICFYKTGAKTSSSLRFVTDKI
                     IEYTDISDFFVKLRTELRKLSQDGR"
     gene            3857..>4629
                     /locus_tag="VLF64_02360"
     CDS             3857..>4629
                     /locus_tag="VLF64_02360"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HSW90818.1"
                     /translation="MNRQHMFSFLHHQKIGLITSTAFAVGAMVGGGVFVLTGVALKQT
                     GPSAIVSFLIAGLIVSFSALSFAVVAAKAGRGESAYAYVGKVLGSPMWGFITSWCFYL
                     GGIVGAAFVLKAFGIYMHSFIYPGLSAISWGLIGAAFLTLVNLGPASQIGRIETLLVG
                     IKLMILLILAAAGIVAFKPHDILPFAPNGTWQIIQTSSYLFVAYLGFSVITSISGDIV
                     RPQKTVPRAIVLSMAIVAVIYAGVVVALLAAHIKDYSEA"
BASE COUNT         1218 a         1077 c         1213 g         1121 t
ORIGIN      
        1 tgcttcaaaa acgattgcaa attattcaag attaactccg tttataattg gcagcctcat
       61 actattatat cgaacattct ggcgagttat gaaattcgta agctgattgg agcgtattcc
      121 gcgagttgca aggggtcaag ctatagtaat aaagttaagt ttattgtata ataagtatcc
      181 atgtctgaac aatttcccaa gtcacgatct aacacctctc ggcaggagca tggtacctca
      241 gaaattcctg gtggatatta ccggtatcag cgtagcgcaa ggcagctcca ggccgagcac
      301 acgggttcac cagatatctt tttagataag tttaaagagc aagagtatgc gcaggttatt
      361 agtgctgcca ttactgctca aaagggtatc gctgacttga tgaaaactaa cccaaagaaa
      421 gcagcaatcg aacaaaagcg ctacgaacct ctctttacag catcagcaga agcgcagcga
      481 gtacttaccg aggctaattt acgatttgcc gcgtattatg ctcgcgcttc aatgaacatc
      541 gttgcgccca aggatgacta tccagcaaaa agacgtcgtg gcgcattcgg cgacataaca
      601 cggctgcgtt cgccatatgc cgaccttgag gaacgcacgc aggttgcaaa cgaggcgctc
      661 gtgaaggctg cacaaacgtt cgttccaaag caagatgaaa aaggcacgcc aatttcattt
      721 ctcgggcacg ccgggtatcg tatccacaag gcactcagta tccatgcgaa aactgaggaa
      781 gcgccaggct ggcatgttag tgcaggcgaa gtagaaaagc tttatgcggc aagaaaggat
      841 ccgacatccg ttgacgcact ggagctggaa cggcttgatg ctatggatga ggggcgtcag
      901 acagtaccga ttgatgaatt cactctagag gtcgtggcgc cagaagaggc agatggtttt
      961 gatgatcttc acgcattgag tcttgccgat gtactgccag accctaatgg cgagccagtt
     1021 gatatcgagg ccctgcttaa tctccgtgac gggagaatac gtaaagtgct tgatacactt
     1081 tccgagcgcg aagctggcgt catctccatg cgttttgggc tggatgacgg aacaatgaag
     1141 aaactcgatg aaatagctgc agtgtatggc gtgacacgtg agcgaatacg acaaattgaa
     1201 tcgaaaacaa tgggtaaatt acggcaccaa agccgaagcc gtcttttaag aggctttgta
     1261 ggtctggaag cacaagactt cgatatagta aatccagcag tacttcccca taaggccgat
     1321 ggtacacaga atatccgcac tacgtacagt gttgcgcatc aagggggtca atccgtggaa
     1381 actgcagaag aagcagagtt cacgcataaa gcctctgaac acatggcttc atggcaggta
     1441 ttccccgatg agccttggga catacaaccg gcgaacgagc gcgtgtcaca aacctttgaa
     1501 gtaaccgctc aacatctgga gcggctgcta tataacaccc caccctacac attccagcat
     1561 tcattcgcaa aggaggtggc ctctcggtac cccatagcgc tcgttgcaac aattaatgaa
     1621 cagcttggca cctcactgac gagcagtcac atcgaagatt tttggaacca tcatatcaat
     1681 aattacgtcg cctcgttgcg tgaacagttg ggagatgatt ttagcctcga cagggtaagc
     1741 gctttgttca caagattact cgcggaatac atgctcgatg atgatcgcgt agaattacac
     1801 atacctcctg aactcgacgg aaaattgcat ggcatgggtg cagagctaca gacgggtgag
     1861 ctgatattat atggaaacgt aggtgatttc actggccacc gaatgaacgg catggcaagt
     1921 attatggtca acggctcaac aggtgatttt actgctttcg agtcggctgg atacgcctca
     1981 cttgaggtta tgggtgatgc tggacatttt gcaggaagca ggcttaccga agccgcgtac
     2041 ctacatgtac atggttcggt cggtgactac tgtggccaag gcttaaaagg catcgacgca
     2101 tacattgagg ttgatggtaa tgccggcagt cacgttggcg acaatgcact ccgcggggtg
     2161 attgatgtac gaggtgtggg tgaaagcgct cagtctaata gtagcgcagt ggtaaatggg
     2221 gaatttgctg aatgggaagc aaaggacacg acgcgggtac cagttagttt gggaaaactc
     2281 gccgttcatc cgccgtatga cacggaggtt attaagcggg tattgactcc gcagccaaaa
     2341 gatcctcaag ctactatcgc tgaaatggaa gctgcagcac aaagcgttaa cccatcattg
     2401 ctcgagcacg ctatgccagc gccaatgcaa gcagagctgt cgccagagcg ccttacgttc
     2461 agccaggcat gcgagtggtt tggcgtatct gccgagcgac ttaacgaatg tttagaggaa
     2521 atcggacata tgagagatgc cgatcttact acctcggaca tgggtgtgat gagtgagctg
     2581 tatgaagact tgcaggtaga aactgtccca acagaaggtt tcatttctac cgaaaaggtc
     2641 attgagaaat ttaaaatccg caaaacgcca acacaattgt tgcgattgct cgctaaaaag
     2701 aatgttccac tctacaaatt tcgcgatgtt gacggttgcg tgaagcactt tataagcaac
     2761 gaaaatctct agcatttgaa ggattaaata acccatgtca actggcggtc ttgatctgcc
     2821 gccgttcgcc tctaaaggga tattgacaaa atcatattga ggttttatta tatcgagtgc
     2881 ctcaataagg ggctattggt agatcgagaa agagaaaggt gggcaggcaa tgcgccagct
     2941 caactttggt tcggttcgcg gcaccctcac gggtctacga cagtcgctcc cagtgtcacc
     3001 atcacgcgca ggcgtgcggg tgcgggggtg ggtgtcatgg cgcttctggg ctgcgtcatg
     3061 tacgcactgc gccagacgcg acgaccgcgc gaagttcagg cctcggccct actgcggtgt
     3121 gtcaccgagg gccctgaggg gtatctcatc gtgcaggtct cgggcaatac gttcctggcg
     3181 ccagtgtcac agcgggctat gatcgagtct ttcaagggct cctcttggcc gatgccgttc
     3241 atgttgctcg ccgtcgagcc catcgacggc ctgcgcgtcg gcacgctgcg ctagggtggc
     3301 gcagcggggt gcggcgcggc ttcgcccgtg ccacaccccg ctctcggtct accagcgtat
     3361 ttccatatct gtgttggtat gctgacgagt cccaaaggac gaaaaatacg aaatgaacag
     3421 tgcagtcaag ctcacctcat aggcgaaaga ggtataataa aactatgaat atctatatct
     3481 cacattgtgg tggcgcttac gactacaaaa cggaacttta cgcgccaatc aaagaatctg
     3541 agcttgctaa gacccattac ttctttttgc cacatgagcc cgaaaacctc gataccgatg
     3601 cagtgacaga gctcaaacgc atggacgtgc ttgtcgccga ggcctcattt ccatcgactg
     3661 ggcagggtat cgaactagcg caggccaagg cggcgaacgt atcgataatt tgcttttaca
     3721 aaactggcgc aaaaacctcg agttcattac gttttgtaac agataaaata atagagtaca
     3781 cagatatatc tgattttttt gtaaagcttc ggaccgaact caggaagctc tcacaggatg
     3841 gtcgctagcc tgaggcatga atagacagca catgtttagc tttctccatc accaaaaaat
     3901 aggacttatc acgtcaactg catttgccgt aggggcaatg gttggtggtg gggtatttgt
     3961 tctcaccggt gttgccttga agcaaacagg gccgtccgct attgtctcat tcttaattgc
     4021 tggccttatc gtatcattct ctgcactttc gtttgcagtg gttgcagcaa aggcgggtag
     4081 aggtgagtca gcatatgctt acgttggcaa agtactcggc tcgccgatgt gggggttcat
     4141 tacctcatgg tgtttttacc ttggcggtat agtgggggca gcgttcgttc tgaaagcgtt
     4201 tggcatctac atgcatagtt ttatctaccc aggcctatcg gctataagct gggggcttat
     4261 tggcgctgca tttcttacac tggtaaatct cggcccagcc tcacaaatcg ggagaatcga
     4321 aacactgctt gtcgggataa aattaatgat cttattaatt ctcgctgcgg cagggattgt
     4381 agcatttaag ccgcatgaca tattgccatt tgcgccaaat ggaacatggc aaattatcca
     4441 aacgagctcg tatttatttg tcgcgtatct aggatttagt gtcatcacga gcatatctgg
     4501 tgatatcgtc agaccgcaaa agacagtccc gcgagcgata gtgttgagca tggcgattgt
     4561 cgcagtgata tatgctggcg tggtggtagc actccttgca gcgcacatta aggattactc
     4621 ggaggcgag
//
LOCUS       DATIIX010000081         1593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_19548, whole genome shotgun sequence.
ACCESSION   DATIIX010000081 DATIIX010000000
VERSION     DATIIX010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19548"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VLF64_02365"
     CDS             complement(<1..413)
                     /locus_tag="VLF64_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90819.1"
                     /translation="MYFESRAQAGQLLAEQLIDKYRYENCAVVALSDGGVLVGEEIAA
                     QLHCILTMLVTEGIEVPGESTMFGAVSQGGNFTYNNEFSTGEIEEYTSEFHGYLDDQR
                     RQAFSRINRLLGDGGIITSDMLRERVVILVSDGLA"
     gene            584..>1593
                     /locus_tag="VLF64_02370"
     CDS             584..>1593
                     /locus_tag="VLF64_02370"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSW90820.1"
                     /translation="MMPVGKQTLLGIVIKEVARPAYTTKEVSAPLDIPTLPIQLVQVA
                     EWMSSYYHAHLATVFQTILPRGLTKKRRFKQREKFVHKRDRTHFLLNETQQAALDTLN
                     NTNAGTVILHGVTGSGKTAVYIEYAKQMLNQGKSVIVLVPEIALTSQLVAEFQQHFPD
                     VVLAHSKQTEAERHTAWLAVAGTITPQVIIGPRSALFLPLKSIGTIIIDEAHEPSYKQ
                     DQSPRYSALRVAGVLAKIHAGIVVQGTATPLISEYFLAKENNRPIIELPERARADAIA
                     PKVALIDMTKRDAFVQHRFLSDELLSKMQASLDANEQVLIFHNRRGSASTTLCENCGW
                     SAT"
BASE COUNT          454 a          431 c          337 g          371 t
ORIGIN      
        1 tcagcaagcc cgtcagacac caaaatcacg acgcgctcgc gtaacatgtc cgaagtaatg
       61 ataccgccat ctccaagtag tcgattaatg cggctgaacg cctggcgcct ctgatcatca
      121 agataaccat ggaattcgct cgtatattct tcgatttctc ccgtagaaaa ttcattgttg
      181 tacgtaaaat taccgccctg agatactgca ccaaacatcg tactctcgcc cggcacttca
      241 atcccctctg tgacaagcat ggtcaagata cagtgaagtt gcgccgcgat ttcctcgccc
      301 actaatacgc ccccatcact aagagcaacg actgcgcagt tttcgtatcg atatttatct
      361 atcagctgtt cggcaagtaa ttggcccgct tgggcccggc tctcaaaata catatgccta
      421 tactaccagt gttctagcct tatctgcaag cggttatatg cgtctcacgg tataatagaa
      481 tgcgatgagg tattatgaga tcgctccgac aaagatcatt cgcgcaaatg cgaatacttt
      541 tacatattca agtagcactc cactcataat agggtcgttc gtaatgatgc ccgtgggcaa
      601 acagacactc ctcgggattg ttataaaaga agtagcacgt cctgcctaca caacaaaaga
      661 ggtttccgcg ccgcttgaca tcccgacgtt gccaatacaa ttagtgcaag tcgccgagtg
      721 gatgagtagc tactatcacg cacatctagc tacggtattt cagacaatac taccccgcgg
      781 actaacaaaa aaacgcagat ttaaacagag agagaaattc gtgcacaagc gagatagaac
      841 acatttttta ctcaatgaaa cacagcaggc tgccctcgat acgctaaaca acaccaatgc
      901 cggaacagtg attcttcatg gagtgacggg tagtggtaaa acagccgtat acatagaata
      961 tgcaaagcaa atgctcaatc aaggtaaatc tgttatcgtc ctcgttccag aaatcgcact
     1021 tacctcacag ctcgttgcag aatttcaaca acacttccca gatgtcgttc tcgcccattc
     1081 taaacaaaca gaagccgagc gccacacagc ctggcttgcc gtagcgggta ccataacacc
     1141 tcaggtcatt atcgggccgc gctcagcgct ctttctgccg ctaaagtcta taggtacgat
     1201 cattattgat gaagcgcatg agccaagcta caaacaggat caatctcctc gctactcagc
     1261 gctccgtgtt gccggggtac tagcaaaaat ccacgctggt atcgtcgttc aaggtacggc
     1321 aacaccactt atcagcgaat atttcctagc gaaagaaaac aaccggccga tcatagagct
     1381 ccccgagcga gcacgagccg atgctatagc cccgaaggtc gctcttatcg acatgacaaa
     1441 acgagatgct ttcgtgcagc atcggttttt atcggatgaa ctgctctcta agatgcaagc
     1501 aagtctcgat gcaaatgagc aggtgcttat ctttcacaac cgccgcggta gcgccagtac
     1561 aacactttgt gaaaactgcg gctggtcggc cac
//
LOCUS       DATIIX010000082         3901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_159929, whole genome shotgun sequence.
ACCESSION   DATIIX010000082 DATIIX010000000
VERSION     DATIIX010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159929"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VLF64_02375"
     CDS             <1..586
                     /locus_tag="VLF64_02375"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HSW90821.1"
                     /translation="RDNGAFTGEVSAAQLHGLVKYALVGHSERRHIFGERDRDLRNKV
                     QAAFRNEITPVLCVGETAHERSDGETNDILHDQLVGGLANITGEEAGQLVIAYEPVWA
                     IGTGSIPTSGDISRAIKSIRSQVKHLFGEATAYNLRVLYGGSVNTDNAANILAIEGVD
                     GLLIGGASLDVHAFSEIIKKAYGEVAVEKKGLKG"
     gene            589..1686
                     /locus_tag="VLF64_02380"
     CDS             589..1686
                     /locus_tag="VLF64_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90822.1"
                     /translation="MADIDFDELDKAVGDLMANVDASKRLPGLDEPEEKTVTIPTSDT
                     ASSVVVPTSTPTVQTVEPSIAAEVAPTPIAASAGRALAVKRRGQFMDIMHPSRDMNTS
                     KPVSRQGATIRPGNDIIAASAAGQNEGLAPEPASPEKQPVEASASAPSAWPDPIDMAT
                     SAAEVPLLPTPTPASETPLEHISETPLMSPFLPDAKPEKRPLGGASQPEDTISVPEIS
                     AVDAQTMPAVALPEEFSGDLMAVESRDLSSHPVQAAVPSQAPVEQPKLEPGEEETLIP
                     AVATPDPIERPQTTTQPPAGGSIAQQYTEQPSSGDQSNGSIYDTSAYHQAIEPTAPAK
                     KKTPMSVWIMIGVGLLLLGAIGGAAYFYFTR"
     gene            1711..>3901
                     /locus_tag="VLF64_02385"
     CDS             1711..>3901
                     /locus_tag="VLF64_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HSW90823.1"
                     /translation="MNILEGLNDAQKSAVLTTGGPLLILAGAGSGKTKTLTHRIAYLL
                     THERVWPNEILAVTFTNKAAKEMRERLWKLTVSAQQVAADNGSSSRLQGEERNSGLSP
                     GLPMESQAPPRNFMPWMGTFHSICVRLLRQDGAAIGIQSNFVIYDEDDRQGLVKQAMK
                     ALSISADQIKPRTVSSAISNAKNELLTPEDYVALAHYPNQEAIGRIYIRYETMRKEAG
                     ALDFDDLLIETVRLLRESIEVRQRYQTQFKHVLIDEYQDTNAAQYAIVKLLVNSAHNI
                     CVVGDDWQSIYSWRGADFKNILGFERDFSGAKVVKLEQNYRSTGAILEAAHNVISKNV
                     ERTDKKLWTDAGKGSPVEIQAVYDETEEAYAVARRISAQTSVGARRHGDFAILYRTNA
                     QSFAFERTLLQYHIPYQIVGGVRFYDRAEIKDIVAYLRLVYQPNDRMSLSRIINIPAR
                     GIGAVSFEKFMEWQASSGMDIITALVNAEQTSKLTPRARNSMVALGEKMRIMQTLVEG
                     GTPPSELIDYLVGAVGYREHILDGTPRAEEREENLGVLISDAKSYATLPDLLEEIALM
                     SSADTDSTESKVTLMTLHSAKGLEFPVVFMVGMEDGLFPSSRALEEGPKQLEEERRLC
                     YVGMTRAREELHLSYAQSRLQFGQRSYASPSRFLEDMGYELMQRQAPIAPQSQDYNEF
                     DEFVMDFDIGDRVRSRQFGDGEIVDIDGLAATIEFDNGVRKKLNVEYA"
BASE COUNT         1050 a          965 c         1015 g          871 t
ORIGIN      
        1 gcgcgataat ggcgcattta cgggtgaggt ctcagcggcg caactacatg gattggtaaa
       61 atatgctctt gttggccaca gtgagcgccg tcatattttt ggcgaacgcg atcgcgacct
      121 tcgcaacaaa gtccaggcgg catttcgtaa cgaaatcacc cctgttcttt gtgtcggtga
      181 aacagcgcat gagcgtagcg atggcgagac aaatgatatt ctccatgatc aacttgttgg
      241 tggtcttgca aacatcaccg gtgaagaagc gggccaatta gtcattgcat acgaaccggt
      301 ctgggcaatt ggcacgggta gcatccctac ttctggcgat attagccgag caattaagtc
      361 gatccgtagc caagtcaagc atttgtttgg cgaggcaaca gcctacaacc tgcgcgtact
      421 gtatggcggc agcgttaata ccgacaacgc agcaaatatt cttgctattg aaggtgtcga
      481 cggcctactt atcggtggtg cgagcctcga tgtgcatgca ttttcagaaa ttattaaaaa
      541 agcttacggc gaagtggcgg tcgaaaagaa aggtctcaaa ggttagtaat ggcggatatt
      601 gatttcgatg aactcgataa ggcagtgggc gacctgatgg caaatgtaga tgcaagcaaa
      661 cgccttcctg gtcttgacga gcctgaagag aaaaccgtga caattcccac gtccgacacg
      721 gcatcttctg ttgtggtccc cacctctact ccaacggtcc aaaccgtcga gccctctatt
      781 gccgcagaag tagctcctac accgatagcc gcgtcagccg ggcgtgcact cgccgtaaag
      841 cgccgcggcc aatttatgga tattatgcat ccatcgcgtg atatgaacac ctctaagcca
      901 gtcagtcgcc aaggtgccac gatccgccct ggtaatgata ttattgcagc aagtgctgcc
      961 gggcagaatg aaggcttagc accggagcca gcatcaccag aaaagcaacc agttgaggcg
     1021 tcggcgtctg caccatcggc atggccggat ccaattgata tggcgacttc tgccgctgag
     1081 gtgccgttat taccaacacc gactccggcc tcagaaactc cccttgagca tatcagtgag
     1141 actcccctga tgtcgccatt cttaccagat gcaaagccag aaaagcgccc acttggtggc
     1201 gcatcacaac ccgaagatac catttccgta cccgagattt cagcagttga tgcacagaca
     1261 atgccagctg tagcgctgcc tgaagaattc agcggtgacc ttatggcggt cgagtcacgc
     1321 gatctatcga gccacccagt gcaggctgcc gttcctagtc aagcgcctgt tgaacagcca
     1381 aagcttgaac cgggcgaaga agagacgctt atcccggcag tagcgacgcc ggatcccatt
     1441 gagcggccac agacgacaac gcaacctcca gctggcggct caattgccca gcaatatacc
     1501 gagcaacctt catcgggtga ccaatcaaac ggttcgatct acgatacgtc ggcgtaccat
     1561 caggcaatcg agccaacggc accagcaaaa aagaaaacac caatgagtgt ctggattatg
     1621 attggcgttg gcctcttgct ccttggtgca attggtgggg cggcatattt ctacttcacg
     1681 cgctaagcat tcttcggtat tatagaggta atgaatattt tagagggttt aaacgatgcc
     1741 cagaaatcgg ccgttctaac gacaggcgga ccattactga tcttagcggg tgcaggcagt
     1801 ggtaaaacaa aaacattaac gcatcgcatt gcgtatctgt taacgcacga gcgtgtatgg
     1861 ccaaatgaaa tcttagcagt aacatttaca aacaaggccg ccaaagaaat gcgtgaacgc
     1921 ctctggaagc taacagtcag cgcgcagcag gtggccgcag acaacggctc ttcgagtcgg
     1981 cttcaggggg aggaacgtaa ttcgggactt agccctggac taccgatgga gagtcaggcc
     2041 cctcctcgaa acttcatgcc atggatgggc acgttccaca gtatctgcgt gcgattatta
     2101 cggcaagacg gtgcggcaat tggcattcaa tcaaacttcg taatctacga cgaagatgat
     2161 cgccaagggc tagtgaagca agcgatgaag gcgctttcaa ttagtgcaga tcagataaaa
     2221 ccgcgaacag tgagctcggc aatcagtaat gctaaaaatg aattactaac cccagaggat
     2281 tacgtcgcct tggcgcacta cccaaatcag gaagccatcg ggcgtattta cattcgctat
     2341 gaaacaatgc gcaaagaggc aggcgcactc gattttgatg atttattgat tgaaacggtg
     2401 cgattgctac gcgaatcaat agaggtgcgc cagcgctacc aaacccagtt caagcacgtg
     2461 ctaatcgatg agtatcagga tactaatgct gcgcagtacg caatcgtgaa gctgctcgtg
     2521 aacagcgcgc ataacatctg tgtcgtggga gatgattggc agtcgattta tagttggcgt
     2581 ggggctgatt tcaaaaacat cttgggcttt gagcgtgatt tctctggcgc gaaggtagta
     2641 aaactcgaac agaactatcg ttccaccgga gctattctcg aagcagccca caacgtaatc
     2701 tcaaaaaatg tcgagcgcac cgataaaaaa ctttggacgg atgccggcaa ggggagtccg
     2761 gtagagatcc aagctgtata tgatgagaca gaagaagcat acgctgtagc gcgccgaatt
     2821 agcgcacaaa cttccgttgg cgcacgtaga catggtgatt ttgctatttt ataccgtaca
     2881 aacgcacaga gcttcgcctt tgaacgcaca ttactgcaat accacatacc atatcagatc
     2941 gtggggggcg tacgatttta tgaccgtgct gaaattaaag acatcgtagc ctacctacgc
     3001 cttgtatatc agccgaatga ccgcatgagc ttaagccgaa tcataaatat acctgcacgc
     3061 ggtatcggtg cagtaagctt tgagaaattt atggaatggc aggccagctc gggtatggat
     3121 atcatcacag cactcgtaaa cgcagaacag accagcaaac tcacaccacg cgcacgtaat
     3181 tcgatggtgg ccttgggtga aaagatgcgt ataatgcaaa cgcttgtcga gggtggcaca
     3241 ccaccgagtg aactgatcga ttaccttgta ggtgcagttg gctatcgcga acatattctc
     3301 gatggcacac ctcgcgcgga agagcgcgaa gaaaacctcg gtgtattgat tagcgacgcg
     3361 aagtcatatg ctacattgcc tgatttactt gaagagatcg cgctaatgag tagcgcagac
     3421 accgatagta ccgaatctaa ggtaacgctc atgacgcttc attctgctaa gggcctagaa
     3481 tttccagtag tattcatggt gggcatggag gacggcttgt tcccaagttc acgcgctctc
     3541 gaggagggcc ctaagcagct cgaagaagag cgccggcttt gctatgttgg catgactcgc
     3601 gcccgggaag aactccatct cagttatgca cagagtcgct tacagtttgg ccagcgaagc
     3661 tatgcatcac cctcacgttt cctcgaagat atgggttatg agctcatgca gcgccaagcg
     3721 ccaatcgcgc cacagtcgca agactacaac gagtttgatg aatttgttat ggactttgat
     3781 attggtgata gggtgcgctc gcgacaattt ggcgatggcg aaatcgttga tattgatggt
     3841 ttggcggcaa caattgagtt cgataatggc gtacgcaaga agctaaatgt cgaatacgca
     3901 c
//
LOCUS       DATIIX010000083         4568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_299543, whole genome shotgun sequence.
ACCESSION   DATIIX010000083 DATIIX010000000
VERSION     DATIIX010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299543"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1358)
                     /locus_tag="VLF64_02390"
     CDS             complement(<1..1358)
                     /locus_tag="VLF64_02390"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HSW90824.1"
                     /translation="MQKWTNNIFRVVATVVITSLVVAPISYAAAGKDFRAKNDVIIYD
                     PDAKKCGDGSNTPLTTEQKIGQILNVGFDNSSIDNLKATAEKYQLGGVYLNIQDPSKL
                     PKKDIDEINTSMKSQLIVATDDEGGQIYRMLPKGAEPSAKQLGKMSDSQVLAAGKDTG
                     TRLNQLGVTTVLGPVLDIDTGLQNAISPYDRSFSSDPNTIADKAGAWADGVTSEKVGV
                     TFKHFPGIGSNTGNTDDTYVVMPNSKSNIDQFKQDLIPYTKLGNKNSASVMLANFVLP
                     GWGDDPVSINPKAVTYLRDTIGFKGLVTTDDLAVMGKSGYGSHQLELPQTIVKALEAK
                     VDMPLFAYPGDAAMDDIVKAVKDNVDLSLIDTAYANVIAYKTSLGLNTAPPAASTGGT
                     ASVVMGGDNRTKIWNFLISNSFKEYGGKPFGPVQAAGAVGNFFQESGWNFADIEVPSG
                     EG"
     gene            complement(1342..1689)
                     /locus_tag="VLF64_02395"
     CDS             complement(1342..1689)
                     /locus_tag="VLF64_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90825.1"
                     /translation="MLNSVSNTNSQQLTSNPPARSNDEVAAAIAKSSPYLHDSKDEPT
                     FKLLKVKRLANNWYMAWIDSTTTKVLINDPSLSTNYMRVLLGPATAFDASDTYSKGIP
                     QQVFGDFVNAKMD"
     gene            complement(1770..2291)
                     /locus_tag="VLF64_02400"
     CDS             complement(1770..2291)
                     /locus_tag="VLF64_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90826.1"
                     /translation="MQDNILTNILSHKKLLIIVGVLFVAALGALGYMQYHKSQPINLK
                     TTNLRNASSEDRAKAAAQLTITINHMPSAIDETTAKYLKNQLAFILRQKYGPDAEKLT
                     ATVRQDLGYDDAGGYSMYVDVPGENETYVAYVNITNHNGSFSCAPQAQQMDPNTSHCY
                     NLPSVDDNAFPNG"
     gene            complement(2291..>4568)
                     /locus_tag="VLF64_02405"
     CDS             complement(2291..>4568)
                     /locus_tag="VLF64_02405"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HSW90827.1"
                     /translation="GDISPTLAANSNNGNFGQAMGREGTDLAGDATKGIGGKVWGYVN
                     ALGPVDTMCTIYNVANTANVLARTVALANIIRFAMTIRATIEKAKAGDDDGESTRYLM
                     DLIQQKDPTTGQSFDETSYAAFLFSGTLSSEPSAVSAIGGQAMLALYMTMHAVHSLFG
                     EVAKYASLGFSGGSAGQGRKFLKDVCKIAQNIGIQIVATAGSFIVGLFSGGSSVAAEE
                     GIMASIKLSFQSAIKTITESVGKTAIKDFLKKQGEKVSEDGLLRYAAKSSWSAFTTLY
                     KSMSPWEKVGLLAAGASAFGMGYIVDTLAGGNIAGFVKNGVSAFMGFGTGWDAYESKN
                     AIASGGTMANYGRAAAYQQTQQTYSNAYVADMQYQAKDTPFDLKNPYSMLGSAMFGIQ
                     KTIGISASLSLPSTLLSMASLPFNIPSLFAAHADDTQPTPKEIGDQVQDPFYQDNSIA
                     VTVTGSPQVIFQKQYSFQDILDKLVDTPNPQVAYTGNDQTTGEPQLSIIPGTDLDAYA
                     QKCHNPERTEADPEFANEEGSNVYDTDTCMQDGKNYKPEYQLYDDAIRFIGQVSPDST
                     DGNSNATTSTAAGTSGQDDVPAKFREIAEGCLKASFGKTACTSDSWLGMLPGQCASFA
                     AWRAAQQWYGALLKSDGSNLEQLLHDKPLPGFGSSLALGNGNQVAPALIANKMADPVK
                     SLADTQPGDIVSVNSSNSAGHVFVILSNNNGQITIEDYNFSGGPGKYGTTTAAGKAIY
                     SQSSVVAIARVHRGGGSN"
BASE COUNT         1016 a         1045 c         1112 g         1395 t
ORIGIN      
        1 tgaccttcac cgcttggtac ttcaatatcg gcaaagttcc acccagactc ctggaagaag
       61 ttgccgactg caccggctgc ttgcactggg ccgaatggtt tgcctccgta ctctttgaat
      121 gagtttgaga ttaagaaatt ccaaattttt gtcctattgt cgccgcccat tacaactgac
      181 gcagtacccc ctgttgaggc tgccggtggt gccgtattga gacctagtga tgtcttgtat
      241 gcgatgacat ttgcataagc ggtatcgata agtgagagat cgacgttatc ctttacggct
      301 tttacgatgt cgtccattgc ggcgtcgcca ggatatgcga agagcggcat atccactttt
      361 gcttctagcg cttttacgat tgtctgtgga agctcgagtt gatgggaacc atatccactt
      421 ttgcccatga cggcaaggtc atcagttgtc acgaggcctt taaagccaat agtatcccga
      481 agatatgtca ctgcttttgg gttaatactc actgggtcgt caccccaccc tggaagtaca
      541 aagtttgcaa gcatcaccga agcactattt ttgttaccaa gcttggtgta tggaataaga
      601 tcttgcttaa actgatcgat gttagatttt gaatttggca tcacgacata ggtgtcgtcg
      661 gtattgcccg tattgctgcc gatgcctggg aagtgtttga atgttacgcc aactttttct
      721 gatgtcacac catcagccca agcgccagct ttgtctgcga ttgtatttgg gtcggatgag
      781 aatgatcggt cgtatggtga gattgcattt tgtaagccgg tatcgatgtc gagcacaggt
      841 ccaagcactg tcgtgacgcc gagttgatta agccgagtgc cggtgtcttt accggctgcg
      901 agtacctggg aatcgctcat cttcccgagt tgttttgcag aaggttccgc gccttttggc
      961 agcatacgat aaatttgtcc gccctcatcg tcggtcgcga cgataagttg agatttcata
     1021 ctcgtgttaa tctcatcaat atcttttttg ggaagttttg acggatcttg gatattgaga
     1081 tagactccgc caagctggta tttttccgct gttgccttga gattgtcgat tgatgaattg
     1141 tcaaatccga cattaagaat ctgcccaatc ttttgttcgg tcgtgagtgg tgtattactg
     1201 ccatcgccgc atttctttgc atcgggatcg tagatgataa catcgttctt tgctcgaaaa
     1261 tccttgccgg ctgcggcata gcttatcggt gcaacgacca ggctcgttat cactaccgtg
     1321 gcaacgacgc gaaatatatt attagtccat ttttgcattt acgaaatctc caaatacctg
     1381 ctgtggtata ccttttgaat acgtatcaga tgcatcaaat gccgtcgctg gcccgagcaa
     1441 tactctcatg tagtttgtac tgagtgatgg atcattaatt aaaactttcg ttgtggtgct
     1501 atcgatccat gccatatacc agttgttggc aagtcgtttt acttttagta acttgaacgt
     1561 cggctcgtcc ttgctgtcgt gaaggtacgg gctacttttt gcgatggctg cggcaacttc
     1621 gtcattactt ctcgctggcg ggttactagt aagttgctgt gagttcgtat ttgatacgct
     1681 atttaacatt gctattacga caataggcac tacgatgagc gatattacga agcctatgat
     1741 aattctgatc gttgtttttg acatagcctt tatccattcg ggaacgcgtt atcatctact
     1801 gatgggagat tgtagcaatg tgaagtattt gggtccattt gttgtgcctg tggcgcgcaa
     1861 ctgaagctcc cattgtggtt tgtgatattt acgtacgcta cgtaggtttc gttttcgcca
     1921 ggcacgtcta catacatact atagccacca gcatcatcat atccaagatc ttggcgaact
     1981 gtggcggtga gtttttctgc atccggccca tatttttgtc gcaagataaa tgctaattgg
     2041 tttttgaggt attttgcggt tgtttcatct atcgcacttg gcatatgatt aattgtaatc
     2101 gtcagttgtg ctgctgcttt agcgcggtct tcgctacttg cattcctcag gttggtggtt
     2161 ttgaggttaa ttggttgact tttgtgatac tgcatataac cgagtgctcc gagcgcagcg
     2221 acaaatagta cgccaacaat gatgagtagc tttttatgac ttaggatgtt tgtgagtatg
     2281 ttgtcctgca ttagtttgac cctcctcccc tatgaacgcg tgcaatcgca acaacgctag
     2341 actgcgagta gatcgctttg cctgctgcgg ttgtagtgcc gtattttcct ggtccaccgg
     2401 agaagttgta gtcctcaatg gtaatctggc cgttattgtt cgaaagaatt acgaacacgt
     2461 ggcctgctga gttgcttgag tttacactta cgatgtctcc tggctgagta tcagccaggc
     2521 tttttaccgg atcggccatt ttattggcaa ttagtgccgg tgctacctgg tttccattgc
     2581 cgagagcaag gcttgaaccg aatcctggaa gcggtttgtc gtgaagaagt tgttcaaggt
     2641 tgctgccatc cgacttaaga agtgcgccat accactgctg ggcagcgcgc catgcggcga
     2701 aggaggcaca ttgacctgga agcataccga gccacgagtc gcttgtacac gctgttttac
     2761 cgaaagacgc ttttaagcac ccttcggcga tttctctaaa tttagctggt acatcatctt
     2821 gcccgcttgt gcctgccgcg gtcgaagttg ttgcatttga attaccatca gtactgtcgg
     2881 gcgatacctg cccgatgaag cggattgcat catcgtagag ttgatactcg ggtttgtagt
     2941 ttttgccgtc ctgcatacag gtatcggtat cgtagacgtt gctaccttct tcgtttgcaa
     3001 actctgggtc ggcttcggtg cgttcgggat tgtggcattt ttgtgcgtaa gcgtcgaggt
     3061 cggtgcccgg aataatacta agctgaggtt cgcctgttgt ctgatcatta ccagtatatg
     3121 ccacttgtgg gtttggagta tcaacaagtt tatcgaggat gtcctgaaaa ctatattgct
     3181 tctggaaaat aacctgcgga cttcctgtta cggtaactgc aatgctattg tcttggtaga
     3241 acggatcttg gacctgatcg ccgatctctt ttggtgttgg ttgcgtgtcg tcggcatgtg
     3301 ctgcaaagag gcttggaata ttgaatggca gcgaggccat ggaaagcaaa gttgacggaa
     3361 ggcttagtga agcagatata ccgatcgttt tctgtatgcc aaacatcgca gagccaagca
     3421 ttgaataagg attctttaga tcaaatggtg tatcctttgc ttggtactgc atatccgcaa
     3481 cgtatgcgtt gctgtaggtc tgttgtgttt gctggtaggc tgcagcgcgc ccatagtttg
     3541 ccatagttcc acctgatgca atagcattct ttgattcgta agcatcccag cctgtaccaa
     3601 atcccataaa cgccgagacc ccattcttta caaatccggc aatattgcca ccggcaagag
     3661 tatcgacaat atagcccata ccgaaggcac ttgcacccgc tgccaagagg ccgacttttt
     3721 cccacggact catactctta tataatgttg tgaaggcgct ccaggagctt ttagcggcat
     3781 agcgcagcag accatcttca ctgacttttt caccttgttt ttttaagaaa tctttaattg
     3841 cggtctttcc gacactttcg gttattgttt tgattgcgga ttggaaacta agtttaatag
     3901 aggccataat tccctcttcc gcagccacac tgctaccacc gctaaagaga cctacaatga
     3961 atgatccggc tgttgcaacg atctgaatac caatattttg tgcaatctta caaacatctt
     4021 tgaggaattt ccttccttgc ccggcgctac cacctgagaa tccaagagat gcatatttgg
     4081 caacttcacc gaagagactg tgaacggcat gcatcgtcat atagagtgca agcattgctt
     4141 gcccaccgat ggctgatacc gcgcttggtt cacttgaaag ggtcccactg aataagaagg
     4201 ctgcgtatga ggtttcgtcg aatgactggc cagtggtcgg gtctttttgt tggataagat
     4261 ccattaaata tcgggtcgat tcgccatcat catcgcccgc cttagccttt tcgattgtgg
     4321 ctcgaattgt catagcgaac cgtatgatat tagcaagtgc aactgtgcgc gccaggacat
     4381 ttgcggtatt ggcgacgtta taaatcgtac acatcgtatc gactggtccg agtgcgttta
     4441 cgtagcccca gaccttacct ccgataccct ttgttgcgtc gcctgcaaga tcggtgcctt
     4501 cccggcccat tgcttgtcca aaattgccat tattgctatt tgccgcaagg gtggggctga
     4561 tgtcgcct
//
LOCUS       DATIIX010000084         1849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_21208, whole genome shotgun sequence.
ACCESSION   DATIIX010000084 DATIIX010000000
VERSION     DATIIX010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21208"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VLF64_02410"
     CDS             <1..188
                     /locus_tag="VLF64_02410"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HSW90828.1"
                     /translation="GEMPELKLPELIVILVIFLLPFGGKKLPELSKSIGQSIRELRGA
                     ATDKDRHDEKAQTEKAE"
     gene            229..1233
                     /gene="tatC"
                     /locus_tag="VLF64_02415"
     CDS             229..1233
                     /gene="tatC"
                     /locus_tag="VLF64_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HSW90829.1"
                     /translation="MEHIHELRGRLVWVALWFVLATGIAFPFFGDIIKLLMKPLGNEK
                     LYYLTPIGGVSFAIKVCMYVGMVATIPVLVYHLYQFISPVMKRRSAKRTILYVSLSTL
                     LAAVGIVFAYLVTLPSAMRFLTGFTIGGVSAMLTVDSYLSFIVAYVLASALMFQVPLI
                     MVIIDRITPMPPSSWNKYQRHMIIGAFIIAMLITPSPEITTQFSLAAPTILMYQIGIL
                     LVWMRHRQVKSEKASASKTLERELVEEGTQPHPQTVLEQQSAPVTAMIAPKPHSGAIP
                     GRVDVLVRYKPTSARNRTVAVPPRQKPQDILGSQARPQISQNPGGRLSIDGFFPASDA
                     "
     gene            1269..1499
                     /locus_tag="VLF64_02420"
     CDS             1269..1499
                     /locus_tag="VLF64_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90830.1"
                     /translation="MNTTKINKGFNVALPKPAILEQEMDRKEFLKNVGIGLLLLLGGN
                     MIMGALKGLEHINSSKQASKNTFGYGGSKYGH"
     gene            1583..>1849
                     /locus_tag="VLF64_02425"
     CDS             1583..>1849
                     /locus_tag="VLF64_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HSW90831.1"
                     /translation="MTERQRAILATIIEQYAEIAAPVGSVTLAKLFGVSSATIRAEMA
                     RLEDMGFIEAPHTSAGRVPTDKGYRVYVNGITDAAMSELPSSLDR"
BASE COUNT          519 a          415 c          437 g          478 t
ORIGIN      
        1 ggggagaaat gcctgaactc aagttgccag aactcatagt aatcctcgta atctttctat
       61 taccctttgg gggtaaaaaa ctaccagagc taagcaagag tatcggtcag tctatccgcg
      121 aacttcgcgg tgccgctact gataaagatc gtcacgatga gaaagcccaa acagagaaag
      181 ctgaataaga cggccccatc taaaacaacg ccaaaaacct tgacgtttat ggagcatatc
      241 catgaattga gaggccggct tgtttgggtg gctctttggt ttgtcttggc aacaggaatt
      301 gcattcccgt tttttgggga catcatcaag ctgttaatga agccgcttgg gaacgaaaaa
      361 ctctactacc ttaccccaat tggcggtgta agttttgcta ttaaagtatg catgtatgtc
      421 gggatggttg cgacgattcc tgttctcgtg taccacctat accaatttat ctcaccagta
      481 atgaagcgtc gcagtgcaaa gcgcaccata ttgtatgtct cgctttcaac actccttgcg
      541 gcggtcggta tcgtattcgc ctatcttgtc acactgccat cggcaatgcg tttcttaaca
      601 ggatttacaa ttggtggcgt gagtgcgatg ttgacggtcg actcgtatct ctcatttatt
      661 gtggcgtatg tactagcgag tgccttgatg ttccaagtgc cattaattat ggtgatcatt
      721 gacagaatta ccccgatgcc tccgtcaagc tggaacaagt atcagcggca tatgatcata
      781 ggcgcattta ttattgcaat gcttatcacg ccgtcgccag agataacaac gcagttttcg
      841 ttagcagcac caacaatcct gatgtatcag atcgggatat tactagtatg gatgcgccat
      901 cgtcaagtca aatctgaaaa ggcgagtgca agtaagacac ttgagcgaga attagtcgaa
      961 gaaggaacgc aacctcatcc tcaaacggtt cttgaacaac agagtgcgcc agttactgca
     1021 atgatagctc ccaagcctca ttcaggagca atcccgggca gagtcgatgt attggtgcgg
     1081 tataagccaa ccagtgctcg taacagaaca gttgcagtgc ctccccgcca aaagcctcaa
     1141 gacatcttag gctctcaagc tcggcctcag atcagtcaaa atcctggcgg tcgactctca
     1201 attgacggtt ttttcccagc aagtgatgca taaacttgca gccactataa gtaagtggta
     1261 ttatgcttat gaatactaca aaaataaaca agggttttaa cgtggcacta cctaaacctg
     1321 caatacttga acaagaaatg gaccgtaaag aattcctcaa gaatgtagga attggcctat
     1381 tattgctact cggtggcaac atgattatgg gcgcattgaa ggggcttgag cacattaaca
     1441 gctcgaagca ggcttcaaag aatacattcg gttatggtgg ctctaagtac ggacattaga
     1501 ctaataaatt ttatgtgcta gggtcgcaaa caactttagc actcatattg atagagtgct
     1561 aagaccttgc tataataact gcatgacgga gcgccagcga gcaattttag caacaattat
     1621 agagcagtat gcagagattg ctgcaccagt tggcagcgtt acacttgcca aattgtttgg
     1681 ggtgagtagt gcgacgatcc gtgctgagat ggcccgtctt gaagatatgg gattcatcga
     1741 agcgccacac actagcgccg gtcgcgtgcc aactgacaaa gggtaccgtg tatatgtaaa
     1801 tggcatcacc gatgctgcaa tgagcgagtt gccaagtagc cttgatcgt
//
LOCUS       DATIIX010000085         2877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_132852, whole genome shotgun sequence.
ACCESSION   DATIIX010000085 DATIIX010000000
VERSION     DATIIX010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132852"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="VLF64_02430"
     CDS             complement(<1..1003)
                     /locus_tag="VLF64_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90832.1"
                     /translation="MSLKKPNVVLVDLGVQAMSFNRARPHWRHFVRRERDPNVGKWRD
                     RRLDEQHASEPELVEYVCKSTDFFDCAEERIVAMLQRRPEMGDLERRIPKLRKRFAVT
                     HEPLDGLIALLAQVPRAVFAQLQMDKYPHGYRNKQERLFELIDFNDTLVSTILLLDDK
                     HREEFSERTKQAADRVCKRIGVPCFTDEQWTAIIRGLTREVAVYSAARSAGFNAWLTD
                     RAHDALGVDLQVQDPEDGRYINIDVKTPSSFRHRMEQLVHEKRLTERELVIGDQQSYV
                     IEQNGHGNNTVQIVVLCILPDLFGDLADWRFVSPEPMRQKINQLIRDHGIHDGQYGKL
                     "
     gene            complement(942..1382)
                     /gene="dut"
                     /locus_tag="VLF64_02435"
     CDS             complement(942..1382)
                     /gene="dut"
                     /locus_tag="VLF64_02435"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HSW90833.1"
                     /translation="MNIAIKKLHEQAVLPKYQTEQAAGMDLVACIDVPVVIEPHQRVI
                     IPTGLAIALPAGYEAQIRGRSGMAARFGVMPANGLGTIDADYRGEIGVILLNTSDEVF
                     TIEPGMRVAQMVIARYEKIVWEVIDELEETKRGAGGFGSTGHVV"
     gene            complement(1383..>2877)
                     /locus_tag="VLF64_02440"
     CDS             complement(1383..>2877)
                     /locus_tag="VLF64_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HSW90834.1"
                     /translation="MPIKEAVEIMQHVPMVGHDTKVILKAFLAVGAKTLPVIAHDTAQ
                     GSFLLNPLRKSRELSDLVGFESIDDAKVAVSAIWALYEEQKPALEVLPALAKVAHTMD
                     FPLIPVLARMEHRGIRLDSGMLAKMDISLSKEITQIQDQMHEMAGHTFNIASPSQLGD
                     VLFTTLQLPTFGIKKGKTGYSTGQKELDKLRGQHPIIELIEQYRELTKLQNTYVQTLP
                     KQVDDKGFIHTTFNQDVAATGRLSSIDPNLQNIPIRTELGRKIRDAFIPEKGNVFVSA
                     DYSQFELRLAAVLAGDEKMTNDFNSGIDIHAKTASDVYHVPLNEVTKEQRRRAKVVNF
                     GVLYGMSQHGLAAAANMSFAEAQHFIDEYFRVRPEVKKYISATIKKAHDEGFVETLFG
                     RRRPTPDVKSSNFVVRAAAERAAANMPIQGTEADLMKLAMIKVDARLDGRGEQVLQIH
                     DSILIECKKEHAGELGKLLEETMEQIYPELGVRLQVDVHTGENWGEV"
BASE COUNT          753 a          792 c          557 g          775 t
ORIGIN      
        1 ataatttacc atactgacca tcgtgaatac catgatcacg aataagttgg tttatctttt
       61 gacgcatcgg ctctggactc acaaatcgcc agtcagctaa atccccaaac agatcaggca
      121 aaatacacag tactacaatc tgcaccgtat tattaccatg gccattctgt tcaataacgt
      181 aactttgctg gtcgccaatc acaagttctc gctcggttaa acgcttttca tgcacaagtt
      241 gctccatacg gtgacgaaat gagcttggtg ttttaacatc aatattaata tagcgcccat
      301 cctcaggatc ctggacttgc aaatcaacgc caagcgcgtc atgcgcgcga tcagtaagcc
      361 aagcgttaaa tccagcactg cgtgccgctg agtacaccgc aacttcacgg gtaagccccc
      421 gaataattgc cgtccactgc tcatcggtaa aacacggtac accaatacgc ttacatactc
      481 gatcagccgc ctgttttgta cgttcgctga attcttctcg atgtttgtca tcaagcagta
      541 aaatcgttga aacaagcgtg tcattaaagt cgatcagctc aaacagacgt tcttgcttgt
      601 tacgataccc gtgaggatat ttatccattt gaagctgggc gaatactgcc cgaggaactt
      661 gtgccaaaag tgcaatcaaa ccgtcaagtg gctcatgtgt aaccgcaaaa cgtttacgaa
      721 gcttcggaat acgtcgctcc agatcaccca tctcgggccg acgttgcaac attgccacaa
      781 tccgctcttc ggcgcaatca aaaaaatcag tgcttttaca cacatattcg acaagctctg
      841 gttcgctagc atgctgctcg tcaagccgcc gatcacgcca tttaccaacg ttcggatctc
      901 gctcacgacg cacaaaatga cgccaatgag gtcgtgcgcg gttaaacgac atggcctgta
      961 ctcccaaatc caccagcacc acgtttggtt tcttcaagct catcgattac ttcccagaca
     1021 attttctcat acctcgcaat gaccatttgc gcgacacgca tgccaggttc aatcgtaaat
     1081 acctcatcgc tcgtattgag caagatcact cctatctcac cacggtaatc ggcgtcaatt
     1141 gtgccaagac catttgccgg catgacacca aaccttgccg ccatgccgct acgaccacga
     1201 atctgcgctt catacccagc aggtagtgca atcgcaagtc cggttggtat aattacacgc
     1261 tgatgcggtt caataacaac aggtacatca atacatgcaa caagatccat acccgccgct
     1321 tgttccgtct gatatttggg caacactgct tgttcatgca gttttttaat cgctatattc
     1381 acctatactt ctccccaatt ctcaccagta tgtacatcaa cttgcagccg tacgccaagt
     1441 tctggataaa tctgttccat cgtctcctca agcagtttac ctagttctcc agcatgctcc
     1501 tttttgcact caatcaaaat tgagtcatga atctgtaaca cttgctcacc gcgaccatcc
     1561 agccgcgcgt ctactttaat cattgctagc ttcataagat ccgcttcggt gccctgaatc
     1621 ggcatattag ccgccgcacg ttccgccgcc gcacgaacaa cgaaattact cgacttcaca
     1681 tcgggtgttg ggcgacgacg cccaaataag gtctctacga aaccttcatc atgtgctttt
     1741 ttaatggtcg cactaatata cttttttact tctggacgta ccctgaaata ttcatcgata
     1801 aagtgctgcg cctctgcaaa actcatgttt gctgcagcag cgaggccatg ttgactcatg
     1861 ccatacaata caccaaagtt caccactttt gcgcgtctac gctgctcttt tgtgacttca
     1921 ttgagcggca catgatacac atcacttgcc gttttggcgt gtatatcgat acccgaattg
     1981 aaatcattag tcatcttttc atcgcctgcc agcaccgcag ctaaacgaag ctcgaactga
     2041 ctgtagtcgg cactcacaaa cacattaccc ttctcaggga taaacgcatc acgaatctta
     2101 cggccgagct cggtgcgaat tgggatattc tgaagattag gatcaatact gctcagtcgc
     2161 cccgtcgcag caacatcctg attgaaagtg gtatgaataa atcccttgtc gtctacctgt
     2221 tttggcaaag tctgcacata ggtattttga agttttgtaa gctcgcgata ttgttcaatc
     2281 aactcaataa tcgggtgttg accgcgcaat ttatcaagct ctttttgacc agtgctatac
     2341 cctgttttgc cttttttgat tccaaatgtc ggcaactgca atgtcgtaaa taacacatcc
     2401 cctagctgtg atgggctcgc aatattgaac gtatgccccg ccatttcatg catctgatcc
     2461 tgaatttgtg taatctcttt cgaaagcgat atatccattt tggcaagcat tccactatca
     2521 agtcgaattc cgcggtgctc catacgcgca agtacaggta tgagcggaaa atccattgta
     2581 tgcgccacct tcgctaaggc aggaagtacc tctagcgcag gtttttgctc ctcgtacagc
     2641 gcccaaatcg cactcactgc cacctttgca tcatcgattg attcaaaacc aacaagatcc
     2701 gacaactcgc gcgatttacg aagcggatta agcaagaacg acccctgcgc cgtatcatgg
     2761 gcaatgactg gtaacgtctt tgcgcccact gcaagaaatg ccttgagaat tactttagta
     2821 tcatgtccaa ccattggcac atgttgcata atctcaactg cttctttgat tggcatc
//
LOCUS       DATIIX010000086         5433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_493614, whole genome shotgun sequence.
ACCESSION   DATIIX010000086 DATIIX010000000
VERSION     DATIIX010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493614"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..275
                     /locus_tag="VLF64_02445"
     tRNA            200..275
                     /locus_tag="VLF64_02445"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:233..235,aa:Phe,seq:gaa)
     gene            368..1147
                     /locus_tag="VLF64_02450"
     CDS             368..1147
                     /locus_tag="VLF64_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HSW90835.1"
                     /translation="MKQPERIVGAIYDVDDTLLVSQPTENPLSNLHQVARLAALHTIA
                     DKYDYCTQLRDVGSQKNFDSFAASPVHTIFGAFHTLLKNRGILAGEVDPMHPLILELV
                     ALKNEEYGKLLPIHGRAIEGAGSFVRDFSKHHSINDFNAIASTAIMRDIKTFLRMTNL
                     GDLFPDNRIIDVGKVAQPKPHPEAFDRAFHSLGLPESARSQVVAFEDDPRGIASARAA
                     DLFVCGITTRYPREFLESLDEAPHLIADSYAEFRKHFGLTN"
     gene            1169..1243
                     /locus_tag="VLF64_02455"
     tRNA            1169..1243
                     /locus_tag="VLF64_02455"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1202..1204,aa:Gly,seq:ccc)
     gene            1345..1725
                     /locus_tag="VLF64_02460"
     CDS             1345..1725
                     /locus_tag="VLF64_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90836.1"
                     /translation="MSMGTIFRLRPFEPKRLLVLPCKGGEITLYVLEGRAYVTNPGGR
                     QIIRAIHFDGWSPSFDNAKLDVPADRLVKSDDAEYCVFDVVFRLVPLEGRGQTAEIVA
                     VSYDGCETFYENELCTLVQEYEGA"
     gene            complement(1886..2668)
                     /locus_tag="VLF64_02465"
     CDS             complement(1886..2668)
                     /locus_tag="VLF64_02465"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90837.1"
                     /translation="MQDGEVITTRYLDALIPGIATELGITCHSFSNNWVHQLARDDAR
                     AEIFGYKFGVNGAAAAAIASDKVATSDLLNHADIPAIKHVLIHNYRGNIESYGSIAGQ
                     VVVKPLSGSGGWGVQRFGSQTEAERAIAESGRFAWAISPYYEIVREIRIIMLDKEILL
                     AYEKTHPVLRDGLRMFNLGAGAEPLDIKPHESLGDMVKAAMKALNLRLASVDVVELHD
                     GKYCIMEVNDGIMMEHYMRQSENHYLRGKAIYTKIMSTLMAK"
     gene            complement(2680..3447)
                     /locus_tag="VLF64_02470"
     CDS             complement(2680..3447)
                     /locus_tag="VLF64_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HSW90838.1"
                     /translation="MGFGITVGIFVAILLLSGIKIVNQFERGVVLTLGRFTGIREPGL
                     RLVVPIFQRMIKVDQRTNTIDIPKQEVITKDNVTVNVDAVVYFRVKSADKAILEVANF
                     VYASSQFAQAALRDVTGNVDLDDLLSKRDEVSQQIEQIVDTETEKWGIDIENVKIQNI
                     ELPQDMKRAMAKQAEAERERRAVVITADGERQAAQGIADAAMTLAKSEGGMNIRTLQT
                     LEKISSEPSQKTLVVLPTDMANSGVAQALTFSESNKR"
     gene            complement(3461..4486)
                     /locus_tag="VLF64_02475"
     CDS             complement(3461..4486)
                     /locus_tag="VLF64_02475"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HSW90839.1"
                     /translation="MTRQTVLLVFGGESSEHEVSVSSAHNIYAAMDGDKYDTKLCFID
                     EQGKWWLLDAWTDNLERHGGVQLVAAPGSKSFMTVPGNTVLHIDVIFPALHGKNGEDG
                     TVQGLAALLGVPIVGCDVTASAVCMDKATTKQVLIASGIKTAKFHLHRRGEELPTYEE
                     IAKDLGDIFFVKPARAGSSVGVSKVHIADEYTPAVRDALRHDDRVLIEEFIDGREFEI
                     SVLGNPPKHQVSGVGEIISGGEFYDYSDKYDEASAAQVLTNIDLSDELRAKLQELASR
                     TYTVLGCRGLSRVDFRMKGSIPYVLEVNTMPGFTNISMYPKLWRAAGMHYPQLVDKLI
                     QLAVKDS"
     gene            4603..>5433
                     /locus_tag="VLF64_02480"
     CDS             4603..>5433
                     /locus_tag="VLF64_02480"
                     /inference="COORDINATES: protein motif:HMM:NF017321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HSW90840.1"
                     /translation="MERVPLAERMRPTTLDEVIGQAHLLGKGEILRQIVQSKEPVSLI
                     LWGPPGSGKTTLARIIAREVEAEFIELSAVTSGKKDVEKVIEHARQNWNLGLRTILFV
                     DEIHRFNKAQQDAFLPHVESGLITLIGATTENPSFEVITPLLSRSRVLVLEPLDKQDI
                     VGIIKRALMNIKKTRNVTPKAIDYLAELSGGDARTALGNLELALSFGEKVTPEIVRAA
                     AQKKVPGYDKKGDMHYDVVSAFIKSMRGSDVTATLYYMSRMIDAGEDPKFIARRMVIF
                     A"
BASE COUNT         1349 a         1403 c         1216 g         1465 t
ORIGIN      
        1 ctcgggtgtt ggctcatcag cataggtaat cgcaacatta tcaagacctc taatatattc
       61 ctgcggcaat tcgagcatcg ctcgctggat tagctggtca aattcgtcat cgcttactag
      121 ctgcattact cctccatcat aacaacttac ccacccgctt gtctatttgc cccttatcta
      181 gtagaataga gcgaagtgtg gctctttagc tcagttggta gagcagaggc ctgaagagcc
      241 tcgtgtcact ggttcaagtc cagtaggagc caccaagaaa agcggatcaa cgaaagttgg
      301 tctgtttttt attaagtaaa gctcacttta cttgcaggcg cctcttcagt catgccatta
      361 tgtggggatg aaacagccag aacgtattgt cggagctatc tacgatgttg atgatacctt
      421 gctcgtgagc cagccaaccg agaatcccct tagtaatctt catcaagtcg ccagacttgc
      481 agcactgcat acgatcgccg ataaatacga ctactgtacg caacttcgcg acgtagggtc
      541 gcagaaaaat ttcgacagct ttgccgcctc tccggtccat acaatcttcg gcgcctttca
      601 tactctgtta aaaaaccgag gcattctcgc gggcgaggtc gacccgatgc acccacttat
      661 cctggagctc gttgctctta aaaatgaaga gtacggtaag ttactaccga tccacgggcg
      721 agcaattgaa ggcgcgggtt cgtttgttcg tgatttctca aagcaccata gcatcaacga
      781 cttcaatgca attgcgagca ccgctattat gcgcgacatt aagacatttc ttcgtatgac
      841 taatcttggt gatcttttcc ctgacaatcg aattattgac gttggaaagg ttgcccagcc
      901 aaaaccccac cctgaagctt ttgatcgtgc atttcattca cttggcctgc ctgaatccgc
      961 gcgtagtcaa gtcgtagcat ttgaggacga cccacgtggc attgcatcag cacgtgcagc
     1021 ggatttattt gtttgcggaa tcactacaag gtacccacga gaattccttg agtcacttga
     1081 tgaagcgccg catctcattg ctgatagcta tgccgaattc cgtaaacatt tcggtcttac
     1141 aaactaaatg gtatactata tagattctgc gggtatcgta tagcggctat tatgtagcct
     1201 tcccaaggct gagatgaggg ttcgactccc tctacccgca ccattataga ctattgaaga
     1261 cgtcgtaacc gacgtctttt gctttatcta atttatgata taatatacgc ggtaatgttc
     1321 ggcaaacgga gaagggctat tactatgtcc atgggcacca tttttcgcct tcgacctttc
     1381 gagccaaagc gtctgctcgt tctgccctgc aagggtggag agatcacgct ctatgtgctc
     1441 gaaggcagag cctacgtgac caatccggga ggtcggcaga tcatccgagc catccacttc
     1501 gacggctggt cgcccagttt cgacaacgcg aagctcgatg ttcccgcgga ccggctagtc
     1561 aagtcggacg acgcggagta ctgcgtgttc gacgtcgtct tccgcctcgt ccccttggag
     1621 gggcgtggtc aaacagcgga aatcgtggca gtcagctacg atggctgcga gacgttctac
     1681 gagaacgaac tctgcactct cgtgcaggaa tacgaggggg cataggaact cgaccgtcgc
     1741 gctccacacc cgatccgctc aagcattttg cctgggggga tcggggcacc acctaactca
     1801 ttgaaaatat cgatggttta tttggtaagg acagccctgg cctataaaaa atagtcctag
     1861 agttttagga ttggattatt ttttgttatt ttgccatgag tgtactcatg atctttgtat
     1921 atatcgcctt gcctcgcagg tagtgatttt cactttggcg catataatgc tccatcataa
     1981 ttccgtcatt tacttccata atgcagtact ttccgtcgtg aagctcgact acatcaaccg
     2041 atgctaggcg taagttgagg gctttcatag cggcctttac catatctcca agcgattcat
     2101 gcggcttgat atcaagtggc tcggcccctg cacctaggtt gaacatcctc aggccatcac
     2161 gcagtactgg gtgcgttttt tcgtatgcaa gaagaatttc cttatcaagc atgattatcc
     2221 gaatctcgcg cacgatctca taatatggtg aaattgccca tgcaaaacga cccgattcag
     2281 caatagctcg ctcagcttca gtttggcttc caaatcgttg cacaccccag ccacccgaac
     2341 cactcagggg tttaactacc acttggccag caatactacc atatgactcg atattgcccc
     2401 ggtaattatg gattagtaca tgcttaattg cggggatatc tgcatgatta aggaggtctg
     2461 aggtcgccac tttatcgctg gctatcgctg ccgctgccgc gccatttact ccgaacttat
     2521 agccaaagat ctcggcccgt gcgtcatctc gtgctaattg atgaacccag ttgttagaaa
     2581 aagagtggca cgttattcca agctcggttg cgatcccagg aatgagcgca tctaaatatc
     2641 gagttgtaat cacttcgccg tcttgcattg aatttaccgc tatctcttat tagactcgct
     2701 gaatgtcaat gcctgcgcga caccgctatt tgccatatca gttggtagca ctacgagcgt
     2761 cttctgactt ggctcgcttg agattttctc gagtgtttgg agtgtacgga tattcatacc
     2821 tccctcgctc ttcgcaagcg tcattgccgc atcggcgatg ccttgtgctg cttgacgctc
     2881 accatctgca gtaatgacta ccgcgcgccg ctcacgctcg gcctcagcct gctttgccat
     2941 tgcgcgcttc atatcttgtg gtagttcgat attctgaatt tttacattct cgatatcaat
     3001 accccatttt tcagtttcag tatcaacaat ttgctcaatc tgctgactca cctcgtcgcg
     3061 cttacttaaa agatcatcga gatctacgtt acccgttaca tcgcgcagtg cagcctgtgc
     3121 aaattggctg cttgcataca caaagttcgc aacttcaaga atcgccttat ctgcactctt
     3181 tacacggaag tataccaccg catcgacgtt gacggtcacg ttatcctttg tgattacttc
     3241 ttgctttgga atatcaatcg tgttggttcg ttgatcaact ttaatcatac gctgaaagat
     3301 tggtacaaca aggcgaagcc ctggttcgcg aataccagta aatctaccta gcgttagtac
     3361 aacgccacgc tcaaactgat ttacaatctt aatgccgctc aaaagcaaaa tagcaacgaa
     3421 gataccaact gttattccaa atcccataat aatttctcct ttacgagtct ttcactgcta
     3481 attgtattag tttatcgaca agctggggat agtgcatccc ggcagcgcgc cacagttttg
     3541 gatacatact gatattcgta aagccaggca ttgtatttac ttcaagcaca tatggaatac
     3601 tgcctttcat gcggaagtca actcgcgaca gaccacggca gcccaataca gtgtatgtcc
     3661 tactcgcaag ctcttgcagt tttgcgcgaa gctcatcact taagtcaata ttcgtcagta
     3721 cctgtgcagc acttgcctcg tcgtacttgt cgctgtaatc gtaaaattct ccgcccgaga
     3781 tgatttcacc aacaccactc acttgatgct tcggcgggtt gccaagcact gagatttcga
     3841 attctcgccc gtcaataaac tcttcaatca atacgcgatc atcatgccgc aaggcgtctc
     3901 ttacggccgg cgtgtactca tcggcaatat gcaccttact aacgcccacc gagctaccag
     3961 cgcgtgctgg cttgacgaag aatatatcac caagatcctt agcaatttct tcgtaagttg
     4021 gcaattcttc accgcgtcgg tgcaaatgaa atttagccgt cttaatgcca cttgctatca
     4081 agacttgctt tgtagtagcc ttgtccatgc acactgcgct cgctgttaca tcacacccga
     4141 cgattggaac gccaagtaat gccgcgagac cctgcaccgt gccatcttcg ccgttcttgc
     4201 catggagagc gggaaatatc acgtcgatat gcaacactgt gttccccggg acggtcataa
     4261 aactcttaga accaggtgct gccactaatt gcacaccacc gtgccgttca agattatccg
     4321 tccaggcgtc aagtagccac cattttccct gctcatcaat gaaacagagt tttgtatcgt
     4381 atttatcacc atccatcgca gcatagatat tatgggccga gctaacggat acttcgtgct
     4441 ctgacgattc tccaccgaat acaagtagta cagtttgtcg agtcattata gttctagtat
     4501 atcgtcaaag ctgaaagctg taaaatgtcc taggcttctg cgaaagcacc cacaccgcac
     4561 gagaacccct ccagggtttc tcgtatccgt tataatagag ccatggaaag agtgccactt
     4621 gcggagcgca tgcgaccaac caccctggac gaggtaatcg gccaggcgca tttacttggt
     4681 aagggcgaaa tcttgcggca aatcgtacag agcaaagaac cggtgagtct tatcctttgg
     4741 gggccgcccg gtagcggtaa aaccaccctg gctcgcatta ttgcccgcga agttgaggct
     4801 gagtttattg agctttcggc agttacaagc ggtaaaaaag atgtcgagaa agtcatcgaa
     4861 catgctcgtc agaactggaa cctagggctt cgtactattt tatttgttga cgaaattcat
     4921 cgctttaata aagcacagca agatgcgttt ttgccgcatg tagaatctgg actcattacg
     4981 ctcattggcg ccaccaccga gaacccaagc tttgaagtta ttacaccact tttaagtcgt
     5041 agccgtgtac ttgtgcttga accgctcgac aaacaagaca ttgttggcat catcaagcgt
     5101 gcactgatga acattaaaaa gactagaaat gttacaccaa aagcgattga ttacttagcg
     5161 gaactttctg gcggtgacgc gcgtactgcc ctcgggaatc tcgagcttgc acttagtttt
     5221 ggtgagaaag tgacgcccga gatcgtgagg gctgcggcac aaaagaaagt tccaggctac
     5281 gacaagaaag gtgatatgca ttatgatgtt gtatcggcat ttataaagag tatgcgtggc
     5341 agcgatgtta ccgccacgct atactacatg agccgtatga tcgatgccgg cgaagacccg
     5401 aagttcattg cccgacgtat ggtcattttt gcc
//
LOCUS       DATIIX010000087         3641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_382916, whole genome shotgun sequence.
ACCESSION   DATIIX010000087 DATIIX010000000
VERSION     DATIIX010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382916"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            560..1642
                     /locus_tag="VLF64_02485"
     CDS             560..1642
                     /locus_tag="VLF64_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90841.1"
                     /translation="MTEGTVVAWTPMMGAMRDLILEALHANGGVIHDEKGRVAGRLRE
                     LIGAPNEKNSNQRISNVLRQLTTEGTIIRIQKEKRTYDIRLVQPCTKDEATALSTAQS
                     DNLSILGAAAPLSEIDRLIHDCQRAYEGLQDAADKATRFMDNGVVVIDPIAVLLAAGI
                     TTRTRAGAIKYYLRRLGLAYSVADAQESDRQRWWWFVGRAEKQPFRAENLRAMATSNT
                     ISYEAQRMAKSGTPWASEVTVRRVEPATALGDADICDELAAATDDAPQDSTTIEQLEP
                     TMAPTFEDDELYKRLVDIAERLERKNERLSAINEELRREAEVQQAAHASEVAALKGEI
                     TVLQKRPALDREILDRAHRLIARNDG"
     gene            1804..2295
                     /locus_tag="VLF64_02490"
     CDS             1804..2295
                     /locus_tag="VLF64_02490"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HSW90842.1"
                     /translation="MAYSTSEEQQELLIELITTIREEMRLAVEWSNAATTYTGLYPTD
                     ATILFFLYEHRSATASEVSKIANLTTGATTAALIRLEKGGFISRARDPNDKRRVIIRV
                     KQLPRQFQVIRKISETELQNSLASTINTTIHDIITHRRLVNTMLKHIINELKTESNKG
                     EIR"
     gene            2292..2711
                     /locus_tag="VLF64_02495"
     CDS             2292..2711
                     /locus_tag="VLF64_02495"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HSW90843.1"
                     /translation="MNNAAISIQIIKDWLDAHNRDDIDSQLTFWDDNAEMTITPTGKV
                     YRGINELRDAAAMAVKSHGRKTLTHIFADDDWVCAEYTATSNVQGPMDAHNIKIPAGV
                     TKELTLQICFLANIQNGKIVRSREYWDTGSMMRQLSA"
     gene            3077..>3641
                     /locus_tag="VLF64_02500"
     CDS             3077..>3641
                     /locus_tag="VLF64_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HSW90844.1"
                     /translation="MENNITDHLDPKANEWLFGVLAKPFKATKHVHSHRDEVYKIETV
                     GANYYLKIAQTLSTERDNLKKLASLLPVPEVVHFRESKEGYDYLLLSELPGKNLVELI
                     GEWSDTEIVETFAQAIRQLHATPVTTVFPEANSRDVVLHGDMALPNILISESKKISYI
                     DFGRLSFGSPELDLADAVWSLQRNLGPV"
BASE COUNT          990 a          953 c          916 g          782 t
ORIGIN      
        1 gccgctacag ccaaccttgg tagcgccgaa tgcataccgt gagggggtgg gctactagta
       61 gcccagcttg gaaaggtcgg tagggaaccc caagttgccc aagttacccg ttcctccctt
      121 gttcttcttg tttcccttcc ccctttttcc cttcttgctc ttgtcgttgc ctgaatcttt
      181 tggggcagga gtggacatga tacccttctt aggttcgaaa tcaggcacgc agaggcgcag
      241 tacccaacct taaatttaat accagctagc agcattggtc aagttttata cttttgaatg
      301 gggtcatttt tgtgcatgat ttccgctcta gcatcccgcg atagggcggc ataccagaac
      361 taagtagagt actgataatc gcacaggaat cactctatct agtagtagtc gcgtcagcaa
      421 ataggcgacg catgtcgcta gaatagcctc cagatcggcg acttggataa gagacattga
      481 aatgttatgc ggtatttacc gttgttaaac aaaatggtat aatatagtgc ggtccaatcg
      541 atgctcggaa ggagcaaaca tgaccgaagg cactgtcgta gcgtggacgc ccatgatggg
      601 cgctatgcgc gacctgatcc tggaggcact gcacgccaac ggcggcgtca tccacgacga
      661 aaaggggagg gtcgcaggcc ggctgcgtga gttgattggc gccccgaacg agaagaacag
      721 taaccagcga atctcaaacg tcctcaggca actcactacg gaaggtacga tcatccgcat
      781 ccagaaggag aagagaacgt acgatatccg actggtgcag ccatgcacca aggacgaagc
      841 caccgcgctt agcaccgccc agagcgacaa cctgtcgatc ttgggcgccg ccgcaccgct
      901 gtccgagatt gaccggctca tccatgactg ccagcgcgcc tacgaaggac tacaggacgc
      961 ggcggacaag gcaacccgat tcatggacaa cggagttgtc gtgatcgacc cgatagccgt
     1021 cctgctggca gcgggcatca ccacccgcac cagggctggt gccatcaagt actacctgcg
     1081 ccggctcggc cttgcgtact ccgtggcgga cgcccaagag tccgacaggc agcgctggtg
     1141 gtggttcgtc ggtcgtgcgg agaagcagcc cttcagggct gagaatctgc gcgctatggc
     1201 tacgagcaac accatatcct acgaagcaca gcgtatggca aaaagcggta cgccatgggc
     1261 cagtgaagtc accgtgcggc gggtagagcc cgccaccgca ctgggcgacg ctgacatctg
     1321 cgacgagctg gcagccgcaa ccgacgacgc gccccaggat tcgacgacca tcgagcagct
     1381 ggagccgacc atggccccaa cattcgagga cgacgagctg tacaaacggc ttgtcgatat
     1441 cgctgaacga ctggagcgca agaacgaacg gctcagtgcc atcaacgagg agcttcgccg
     1501 cgaagctgaa gttcagcagg ctgcccacgc aagtgaggtg gccgccctga agggtgagat
     1561 caccgtgctg caaaagcgac cggccctgga tcgagagatc ctcgaccgcg cccaccgtct
     1621 catcgctcgc aacgacggct gaccccttca ccatcttccg caccggcccc agcgccggtg
     1681 cggaagatag cacaacctaa gatattactt gccacgcttt tgcttggctt ttttatttct
     1741 agaaaataca aagaatgata gaacatagtc aaataattac ctttatgata aagttatttt
     1801 accatggctt acagtacatc agaagaacag caagaattat taatagagct tattactact
     1861 attcgcgaag aaatgcgtct tgcggtggag tggagcaacg ccgcaacaac gtataccggt
     1921 ctttatccca ccgatgcaac tattttattt tttctttacg aacaccgttc tgccacagcg
     1981 agcgaagtaa gcaagatcgc taacctcaca acgggcgcaa caactgcggc gctcattagg
     2041 cttgaaaagg gcggatttat tagtagggca cgcgacccta acgacaaacg ccgtgttatc
     2101 attcgcgtaa agcagctgcc aaggcagttt caagtcattc gcaaaatctc tgagacagaa
     2161 ctgcaaaaca gtctagcaag cactattaat acgacaattc acgatattat tacacaccgc
     2221 cggcttgtta ataccatgct caagcacatc attaacgagc taaaaacaga atcaaataaa
     2281 ggagaaatac gatgaataat gcagcaataa gtattcaaat tatcaaagat tggcttgacg
     2341 cacacaacag ggacgacatc gacagccagc tcacattttg ggatgacaac gccgagatga
     2401 ctatcacacc aacaggcaaa gtctatcggg gtatcaacga gttgcgcgat gccgcagcta
     2461 tggcggtaaa atcccatggt cgcaaaacac taacgcatat tttcgccgac gacgattggg
     2521 tatgcgccga gtacacggca acatctaatg tacaaggccc gatggacgcg cataatatta
     2581 aaatccccgc gggtgtcaca aaagagctta cgttacaaat ttgcttcttg gcaaatatac
     2641 aaaacggtaa aattgttcgc tcccgtgaat attgggatac gggcagtatg atgcgccagc
     2701 tgagtgccta gaaggtccta ggggcgtcgt aacggctcgc agttcgtggg cctagtttag
     2761 cacttttcac aaatgctaaa ctgggctcac gaccgtgggc tctatcgtcg agtggcatag
     2821 gagtcactgt gaacatcgca aggagatcgc gaaggcgagc aaaagcgcta cgacagtctg
     2881 cgctcaagcg cagatcgatc atgtatgtgg attgcggcaa aacaaattgc cgcatcggca
     2941 taatcgacac gaacggcttg ccgatccagc agatgctgaa ctacgacgtg actaagttca
     3001 atacccataa attcgcctgc tcatgtacgc cacgtcgcgc atatgataga aaacggtaat
     3061 acgatataat tgtaaaatgg agaataatat tacagaccat ctcgacccca aagcaaatga
     3121 atggctattt ggcgtgcttg ctaaaccgtt taaggccacg aaacatgtcc attctcatcg
     3181 ggatgaggta tataaaatag aaaccgtagg cgctaactac tatcttaaaa ttgcacagac
     3241 gctttcaacg gagcgggata atctcaaaaa gctcgcatct ctcctgcctg ttccggaagt
     3301 agttcacttt cgtgaatcaa aagaggggta cgactacctg ttactcagcg aattaccagg
     3361 taagaatctt gttgaactca ttggagagtg gtctgacact gaaatcgtgg agacttttgc
     3421 gcaggctatc aggcagctcc atgctacacc cgtaactacc gtatttcccg aggccaattc
     3481 tcgcgatgta gtactccacg gcgatatggc attgccgaat attctcattt cagaatcaaa
     3541 gaagattagc tatatcgact tcgggcggct cagcttcggt tctcctgaac tagatttagc
     3601 cgatgccgta tggagcttgc aaagaaacct tggtcccgtc t
//
LOCUS       DATIIX010000088         3569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_522715, whole genome shotgun sequence.
ACCESSION   DATIIX010000088 DATIIX010000000
VERSION     DATIIX010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522715"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VLF64_02505"
     CDS             <1..904
                     /locus_tag="VLF64_02505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSW90845.1"
                     /translation="RPFKMIPNFALARGVVAREDLRATISGETIRLSVIYSPIRSNNS
                     SSSQKSYNDGFVVILRDITKQKSLEEERDEFISVVSHELRTPITVAEGSLSNLEFMME
                     RTDIPQSVLRENIKMSHDQVLFLARMVNDLSTLSRAERGVADAAEEISVDELVQDLFK
                     EYARQAEAKGLRFNLDVEPQLGEVSASRLYLKELLQNLITNAIKYTKEGEVTLIAKKL
                     KGNKLELTVKDTGIGISKSDQAHIFEKFWRSEDYRTRETGGTGLGLYVARKLAKKLGI
                     EIEMASRLNHGSTFKFIIMTNKKD"
     gene            991..1359
                     /gene="secE"
                     /locus_tag="VLF64_02510"
     CDS             991..1359
                     /gene="secE"
                     /locus_tag="VLF64_02510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HSW90846.1"
                     /translation="MAKAPTKKDSDASTKVTRIKASESSIKKATQAKAKPTIDSKKKE
                     AIAKIENREQTSSRNPFRAIANYFKGSWYELTQVRWPDRKSTWGMTGALVAFTLFFVV
                     VVLLFDYGFAQLFKLILGSN"
     gene            1363..1911
                     /gene="nusG"
                     /locus_tag="VLF64_02515"
     CDS             1363..1911
                     /gene="nusG"
                     /locus_tag="VLF64_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HSW90847.1"
                     /translation="MAKLNRYDSTRQWYAIHTYSGYEEKVADSIRQRIQAVDMADKIF
                     DAMVPKEKQIQIKNGKRKVIEAKIFQGYVLVEMKLTDETWYIVRNTPGVTGFVGSGTE
                     PTPVSDSEITKIKRRMGVEDPKHQIDFSVGEVVNIVDGPFKGFDGAVSEIDTAKGKLK
                     VMVSMFGRDTPVELDALQVSKV"
     gene            2023..2448
                     /gene="rplK"
                     /locus_tag="VLF64_02520"
     CDS             2023..2448
                     /gene="rplK"
                     /locus_tag="VLF64_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HSW90848.1"
                     /translation="MAKKVIGNLKLRIPAGRATAGPPVGSTLGQWGLNMMDFINPFND
                     ATKADMGKDVIVHIQVFEDRPFTWKSLGQPVDDMIREAIGIKKGSGKPHAEKVGKITR
                     AQLEEIAQAKMGQLNAIDLDGAIKVIAGTARSMGVEVTN"
     gene            2546..3463
                     /locus_tag="VLF64_02525"
     CDS             2546..3463
                     /locus_tag="VLF64_02525"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HSW90849.1"
                     /translation="MRKSVERQQHAKAVAETIHTSRVAHHRRLAAIIILALIILDTAI
                     IGIFQLQANQTIAATQKKFASDQTVVAVNKPFTHILASVRQTEAHSAEQTVKSIPAYP
                     QKVITNTTSITATAHCDVNNPTSILVIVNKKHCINPPSWAPPDLASLDGYLMRSIAAT
                     HMQEMMQAATAAGLPFGITSAYRSYDDQVATYNNWVQVNGSTASADTVSARPGYSEHQ
                     TGLAADLKTDGCVLDCFTGTAQYTWLQQNAANYGFIQRYPTSLTSITGYSAEPWHWRY
                     VGSDTALAMKRSGVQTLEEYLGVSGGDYN"
BASE COUNT         1069 a          903 c          804 g          793 t
ORIGIN      
        1 cagaccattc aaaatgattc caaattttgc acttgcacgc ggcgttgttg ctcgcgaaga
       61 tttacgcgca acgatttctg gcgaaacaat ccgtcttagt gttatttact caccgatccg
      121 cagtaacaat agctcgagta gccaaaaatc ttacaatgac ggcttcgtgg taatcctccg
      181 cgacattact aagcaaaaat ctcttgaaga agagcgtgat gaatttattt ccgtcgtcag
      241 tcacgagctt cgcacgccaa tcacagtcgc cgaaggatct ctttcaaatc tcgagttcat
      301 gatggagcgt accgacatac cgcagtccgt ccttcgtgaa aacatcaaga tgtcgcacga
      361 ccaagtactg tttcttgcgc gcatggttaa cgatctaagc acgctcagtc gcgcagaacg
      421 tggtgtcgcc gatgcagccg aagagattag tgtggacgaa ctcgtacaag acctctttaa
      481 ggaatacgca cgccaagcgg aagctaaggg cctccgcttc aatcttgatg tcgaaccgca
      541 acttggcgaa gtaagcgcaa gtcggttgta tctcaaggaa ctacttcaga acctcattac
      601 gaatgctatc aagtacacga aagaaggtga agtcacactc atcgccaaaa aactcaaggg
      661 caataaactt gagctcaccg taaaagacac tggcattggt atatccaagt cagatcaagc
      721 acatattttc gagaaattct ggcgcagtga agattaccgc acacgcgaaa caggtgggac
      781 aggacttggc ctctacgtcg cacgaaagct cgcaaaaaag ctcggcatag aaatcgaaat
      841 ggcctcgcgc ctcaatcacg ggtcaacgtt taagttcatt attatgacca acaaaaaaga
      901 ctagggcttg acgatttacc taatactcac tataattgac ttgacagtct ggtgagtaaa
      961 ccaggctttt aattttggga gacacacgga gtggcaaaag caccaaccaa gaaagactct
     1021 gacgcaagca ccaaggtgac tcgcatcaaa gcaagcgaaa gtagcataaa aaaggctaca
     1081 caggctaaag ctaagccgac tatcgatagc aagaaaaaag aagctatcgc taagattgaa
     1141 aatcgcgaac aaacaagtag ccgcaatcca tttcgtgcga ttgcaaacta ctttaaaggc
     1201 tcttggtacg aacttacaca agttcgttgg ccagaccgca aaagcacgtg gggtatgacc
     1261 ggtgcgctcg ttgcgttcac gctctttttc gttgttgttg tcctcctctt tgattacggc
     1321 tttgcacaat tatttaaact aatcctcggg agtaactaat ctatggcaaa actaaaccgc
     1381 tacgactcaa ctcgtcaatg gtacgcgatt cacacgtact ctggctatga agaaaaagta
     1441 gccgatagca tccgccagcg catccaggca gtcgacatgg ctgacaagat ttttgacgcg
     1501 atggtgccaa aagaaaagca aatccagatc aagaacggta aacgcaaagt tattgaagcc
     1561 aagatcttcc agggctacgt gctcgttgag atgaaactca ccgacgaaac atggtacatc
     1621 gtgcgcaaca ccccaggcgt taccggcttt gtcgggagcg gcactgagcc tactcccgta
     1681 tctgacagcg aaatcaccaa gattaagcgc cgcatgggcg tagaagaccc taagcatcaa
     1741 atcgacttca gcgtcggtga agttgtaaac atcgttgatg gtccattcaa gggcttcgat
     1801 ggtgcagtca gtgagatcga caccgccaaa ggcaaactca aggttatggt gagcatgttc
     1861 ggccgcgaca ctccagttga actcgacgcg ctacaggtga gtaaggtcta gtaacagcgc
     1921 tcatatcttg catttacagg taatttagag tataattaac caccgttacg cactgcgaaa
     1981 tgcttcaaat ttagcagtaa gaaataaagg acaatattta ctatggcaaa aaaggtaatt
     2041 ggtaacctca aactccgtat ccctgccggt cgcgcgactg ctggtcctcc agtaggtagt
     2101 acactcggtc agtggggcct taacatgatg gatttcatca acccattcaa cgacgctact
     2161 aaagcagaca tgggtaaaga tgtcatcgtg cacatccagg tttttgaaga ccgccccttc
     2221 acttggaaaa gccttggcca accagttgac gatatgattc gcgaagctat cggcatcaaa
     2281 aagggcagtg gtaagccaca cgctgaaaag gtaggtaaaa tcactcgcgc acagctcgag
     2341 gaaattgcac aagctaagat gggtcagctt aacgcgatcg atcttgacgg tgccataaag
     2401 gttattgccg gcacagcacg cagcatgggc gtagaagtca ccaactaatt acaccaagct
     2461 tacaataaaa tacgaagcta ctatagagct tcgtatttta tttgccgccc aagcgaggca
     2521 taatgggata tagtatactg aatatatgcg aaaatcagtc gagcgacaac agcatgcaaa
     2581 agcagtcgcc gaaactattc ataccagccg cgtagcccac caccgtcgcc tcgcagcgat
     2641 aattattttg gcgcttatca tccttgacac cgccattatt ggcatatttc aactgcaagc
     2701 aaatcaaacc atagctgcta cccaaaagaa attcgcgagc gatcaaacgg tagtcgccgt
     2761 aaataaacca ttcacgcata tactcgcttc tgtgcgccaa acagaagccc atagcgccga
     2821 gcaaacggtt aaatccatcc ccgcatatcc tcaaaaagtt attacaaaca ctacttcaat
     2881 tacagcaaca gcgcactgcg atgtcaataa cccgacatca atccttgtaa tcgtcaacaa
     2941 aaaacattgt atcaaccctc cttcatgggc tccaccagat ctcgcatctc ttgatggcta
     3001 cctgatgcgc agcatcgcag ccacgcacat gcaagaaatg atgcaagctg cgaccgccgc
     3061 aggactgccg tttggtatta caagtgcata tcgctcatac gatgatcagg ttgcaacata
     3121 taacaactgg gtacaggtaa atggcagtac ggcatcagca gacacagtaa gcgctcggcc
     3181 aggctacagt gagcaccaaa cgggccttgc cgcagatctt aagaccgacg gatgcgtgct
     3241 cgactgcttt acaggcaccg cccagtacac gtggctacaa cagaatgctg caaactacgg
     3301 cttcatccaa cgctatccta ctagccttac aagcatcact ggctactcgg cagaaccatg
     3361 gcattggcgc tatgtaggca gtgatacggc gctcgcgatg aaacgaagtg gggttcagac
     3421 gcttgaagag taccttggcg tgagtggggg tgactacaac tagtccgtaa tgttcgctga
     3481 tggtgcgcgg ttcacgcgta caatttcagt atcattaata ccctccacac gttctggcgt
     3541 tctcgaacca cacacttcag cttagatcg
//
LOCUS       DATIIX010000089         1887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_355012, whole genome shotgun sequence.
ACCESSION   DATIIX010000089 DATIIX010000000
VERSION     DATIIX010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355012"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /gene="rsmD"
                     /locus_tag="VLF64_02530"
     CDS             complement(<1..419)
                     /gene="rsmD"
                     /locus_tag="VLF64_02530"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HSW90850.1"
                     /translation="MTFVRVVAGKFGGRKIDAPSFDNSRTKPMGERIRNAIFNRVGNE
                     IKGAHVLDAFAGTGAVGLEALSRGAAHVTFIERDKIAQKIIAKNMVLLGCEDNATLVS
                     TSVNNWLETTTPESYDIIFADPPYHDPQLSTVEKLFSL"
     gene            complement(416..1222)
                     /locus_tag="VLF64_02535"
     CDS             complement(416..1222)
                     /locus_tag="VLF64_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90851.1"
                     /translation="MYAGTTFHGKSGGFIGVHQKIDRIARRNFNTLPGVHKDFPGIRE
                     ILHFEGNNGPDGIKRKSPSTDEPWHYIDPDNVNDRKILEMILDHQHNLATALKQNNSE
                     RAAFEAAWMAHAITDGLTPAHHYPLSDKIEELWGKPKEERLTVRDKNIIKGEGARDTL
                     SRNWEYWGVKGVFMTHGLFELGVATSIKTTRFTQSAPTAEWLKQADKKGLEQVFLDTM
                     HEIHGLAMYERFWKQGWNANLARETRQLLIPKICAVISFAWYTAYREAGL"
     gene            complement(1257..>1887)
                     /locus_tag="VLF64_02540"
     CDS             complement(1257..>1887)
                     /locus_tag="VLF64_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HSW90852.1"
                     /translation="QAYVICSLIADSSENDRKSVEAEYKRLKNSIFGHCSIGLLHGKM
                     KPAEKEQVMQEFKDRKYDILVSTTVVEVGVDVPNATAIMIEDAEQFGLAQLHQLRGRV
                     GRSELQSYCYLMMGTTNKPSERLRELEKSNDGFYLAEVDMTLRGPGEIYGKRQSGALN
                     LQIATLADTELIARAQKAAKWFIESGEDITAYKELNAQVKHYQRLTTLN"
BASE COUNT          432 a          457 c          444 g          554 t
ORIGIN      
        1 agactaaata atttttcaac tgtggataac tgtgggtcgt gatatggcgg gtcggcaaag
       61 attatgtcgt aactttcggg agtcgtggtt tcgagccagt tatttactga tgtacttacg
      121 agcgtcgcgt tgtcttcgca ccctagtaat accatatttt tggcaatgat cttttgcgct
      181 attttgtcac gctcgatgaa tgtcacatgt gcggcaccgc gactaagtgc ctctaacccg
      241 accgcgccag tacctgcaaa tgcatcgagt acatgcgcac ctttgatttc attgcccacg
      301 cgattaaata tagcgttgcg aatgcgctcg cccatcggct tagtgcgtga gttatcaaag
      361 cttggggcgt caatcttgcg accgccaaat ttaccggcga ctacgcgtac aaatgtcata
      421 gtccggcctc gcgataggcg gtataccagg caaagctaat tacagcacaa atctttggta
      481 taagtagctg gcgggtttcg cgcgccaggt tggcgttcca gccttgcttc cagaatcgct
      541 cgtacatagc aaggccatga atttcatgca ttgtatcgag aaatacttgc tcgaggcctt
      601 ttttatctgc ttgtttgagc cactcagctg ttggcgcgct ttgtgtgaaa cgagtagttt
      661 tgatagaggt cgccacacca agctcgaaga gtccatgggt cataaacaca cctttgacac
      721 cccaatattc ccagttgcgg ctgagcgtat cgcgtgcccc ctcgcccttg atgatgtttt
      781 tgtcacgcac tgtgagtcgt tcttcttttg gtttgcccca gagttcctca atcttgtcac
      841 tgagcggata atgatgggct ggggttaggc catcagtgat tgcatgtgcc atccaagcag
      901 cctcaaacgc agctcgctca gagttatttt gtttaagcgc cgttgctaaa ttgtgttggt
      961 gatcgagaat catctctaag atcttgcggt cattcacatt atctggatct atataatgcc
     1021 atggctcatc cgtgcttgga cttttgcgct taataccatc tggcccatta ttgccttcaa
     1081 agtgaagaat ctcgcgaatg cccgggaaat ccttgtgaac gccaggaagt gtattgaaat
     1141 tgcgcctcgc aattcggtca atcttctgat gaacgccaat gaatccgccg gatttgccat
     1201 gaaaagtcgt tcctgcgtac attagtattt atagtagcaa attgccgagg taaatgctaa
     1261 ttgagtgtcg tgaggcgctg gtagtgcttt acttgggcat tcagttcttt ataggccgta
     1321 atatcttcac cgctttcaat aaaccatttg gccgcctttt gagcccgagc gataagctca
     1381 gtgtcggcaa gtgtagcaat ttgtagatta agcgcacctg attggcgttt gccatagatt
     1441 tcgccggggc cacgaagtgt catgtcgact tcagcaaggt aaaatccatc attggatttt
     1501 tcaagctcgc gcagtcgctc acttggttta tttgtcgtgc ccatcataag gtaacaataa
     1561 ctttgcagct cgctacgtcc tacacgcccg cgtaactggt ggagctgagc aaggccgaat
     1621 tgttcggcat cttcaatcat aattgccgta gcattcggca cgtcgacgcc aacctcaaca
     1681 accgtcgtgc ttacaagaat atcgtacttt ctgtctttaa attcctgcat cacctgctct
     1741 ttttcggcgg gtttcatctt gccatgcagc aggccaatgg agcaatgtcc aaaaatgcta
     1801 ttcttaaggc gcttgtattc tgcttcgaca cttttacggt cattttcgga tgagtcagcg
     1861 attaaactgc aaattacgta tgcctga
//
LOCUS       DATIIX010000090         4891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_523524, whole genome shotgun sequence.
ACCESSION   DATIIX010000090 DATIIX010000000
VERSION     DATIIX010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523524"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..702
                     /locus_tag="VLF64_02545"
     CDS             244..702
                     /locus_tag="VLF64_02545"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HSW90853.1"
                     /translation="MYKKLAVAGFVIVTFIIYSLHQRSEDSQAVQKVSIRQNATPQSN
                     TSSANPLTTVTTAVDYKDGAYTGKSSDAFYGYVQVRATISGGKLSNITILDYPQDRDN
                     SVEINNSALPQLKQEAIQAQTAQIDGVSGATDTSQAFKESLGDALAQAKA"
     gene            704..1447
                     /locus_tag="VLF64_02550"
     CDS             704..1447
                     /locus_tag="VLF64_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HSW90854.1"
                     /translation="MIKETRLIMGMPIILMTPDELVLRENIEEVFSFFTYVDKTYSPF
                     IESSLVSRISRGEVDEPEYSNELHEILAVARQTKHETNGYFDVWHKGDFDPSGIVKGW
                     AIAEAAKIIGRYTNDFYIEAGGDIQVGGANEEGGPWQIGIRNPFDRHQNIKVVALKNS
                     AIATSGTAIRGQHIYDPVDNKKLNEIVSISVIASNIIDADRMATAAFAMGVKGIYFIE
                     QLGGYEACAVTSDKTTITTTGWQKYEENE"
     gene            1444..2952
                     /locus_tag="VLF64_02555"
     CDS             1444..2952
                     /locus_tag="VLF64_02555"
                     /inference="COORDINATES: protein motif:HMM:NF015100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HSW90855.1"
                     /translation="MNYVDNMINRITMYRFLIYYLCGLLLVAMFFGAIGTLAYPPQMI
                     LLSASITVGVCYAVNYVFAKIYDAPSNTESALITGLILSLIITPMSSQKGLPFLVAAS
                     GLAIASKYIIAIGNKHIFNPVAIAVVLTAFGPGESASWWVGSVALLPYVIAGGLLIIR
                     KIRRATMFAIYFVAAICSVIIMAAATNHDIVTNFQATLQHSSLFFLGFVMLTEPWTSP
                     STKRHRYIYATIVGLLFSPLIQIAGIYSTPELALILGNLYAFLVTPIAKTKVRIIHRT
                     MYGANTEDIELAPEHPFLYKPGQYIEMTLPHHGSDARGARRYFTLASSPTEDTLHLGV
                     RYYNNGSSFKETLHTSENALMSFGQVGGDFTLPSDASARLAFIAGGIGVTPFRSMVKY
                     LSDTNDQRSAILLYGERSASDVAYKEIFEEARAKIGLTTKYILSASLQTQDNEILGNI
                     SRELIEREIPDYASRMFYISGSQSMVQGVRRELRAMGVARRNIKADYFFGYA"
     gene            2978..3496
                     /locus_tag="VLF64_02560"
     CDS             2978..3496
                     /locus_tag="VLF64_02560"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HSW90856.1"
                     /translation="MDIFIVFCAKYLIIIIGLLALGYWLTLPRKQKTEVLIMGAVTGI
                     IAFILAKTGSAMFTDPRPFVSDGVTPLFAYTADNGFPSDHTLLGMIVTMALLSVSRKW
                     AINLFVLTVIVGASRVLAGVHHPVDIVGSIVFGALGGVVAVYVTPKIIDWLATSRYAK
                     SLGLHHISKKKK"
     gene            3605..3934
                     /locus_tag="VLF64_02565"
     CDS             3605..3934
                     /locus_tag="VLF64_02565"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HSW90857.1"
                     /translation="MIDTKLTSDLLRGHTDTMILKLLIDGDRYGYEITKHILDASGGE
                     YELKEATMYSSLKRLETDGRISSYWGDESQGGRRKYYHITESGREVYDTNITTWNAAK
                     KILEKLL"
     gene            3945..4781
                     /locus_tag="VLF64_02570"
     CDS             3945..4781
                     /locus_tag="VLF64_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HSW90858.1"
                     /translation="MNDKLIQFLDNAFKPYGNFPARKDVEQELLANLTEKYSDLKRDG
                     KSDEEAYQLTTESFGDVSEIMEQVAPEEHARETRKQDLRKTIVDSIKDTFGIGDTSRF
                     RASALEESDLADVNLRGADFSMSAMMKSNFNGSQLQNAKFKAAALKGSTFTGADLTGA
                     EFNSSDIQESDFTNANLEGATLISCALKGANFNSANLTNTVFKHSDLSEVVFDGHMLN
                     GTVFDGAGLKNTSFQGATLVNVSFHHSRVKYTIFDGATMDKTTNALLKGAKANLDNVT
                     VE"
BASE COUNT         1448 a         1076 c         1116 g         1251 t
ORIGIN      
        1 tacatatgct aattttggtg gatgtggctg gttatagagg aaaagtgcac caattataat
       61 gacacagaca gtggcaattg cgctcactgt gagctgttgt ttatgcatag taatgtaggt
      121 cataactttc tcgaggtagt gtttcataat tagttacagt atagcagtag tgatgactat
      181 acagctaatg aatattctga tactttaagc ttatattaac gcacatcaat ttaactagac
      241 agtatgtata agaaattagc cgttgctgga tttgtcattg tcacgtttat tatctatagt
      301 cttcaccagc ggagtgagga ctctcaggcg gtacaaaaag tctcaatacg tcaaaacgcc
      361 acaccacaga gtaatacttc tagcgcgaat ccactcacta cagtcacaac cgcagtggac
      421 tataaagacg gtgcttatac aggcaaaagt agtgatgcat tctacgggta tgtgcaagtg
      481 cgcgcaacaa tctcaggtgg taagttgagc aatattacca tcctagatta tccccaagac
      541 cgtgataatt cagtcgagat taacaacagt gcacttccac aactaaaaca agaagcgatc
      601 caagcgcaaa ccgcacaaat tgacggtgtc tcgggcgcaa ccgacaccag ccaggcattc
      661 aaagaatcac tgggcgacgc gctcgcacag gcgaaggcct aacgtgataa aagaaacgcg
      721 gcttattatg ggcatgccaa ttatactcat gacacctgat gaattagtat tgcgcgaaaa
      781 tatcgaagaa gtcttctcgt tttttaccta tgttgataaa acgtacagcc cattcattga
      841 atcgagctta gtgtcgcgta ttagtagagg cgaagtagac gagccggaat attccaatga
      901 gttgcatgaa atactggcag ttgctagaca aacaaaacac gaaacaaatg gatattttga
      961 tgtatggcac aaaggcgatt tcgacccttc aggcatcgtg aagggttggg cgattgctga
     1021 ggcggcgaag atcatcggca gatatacaaa cgacttttac attgaagcgg gcggcgatat
     1081 tcaggtgggc ggagcgaacg aagaaggcgg cccatggcag attggcatac ggaacccttt
     1141 tgatcggcac caaaatatca aagtagttgc cctcaaaaat agtgcaattg ctacatcagg
     1201 aacggcaatt cgtggacagc atatctacga cccagtcgat aacaaaaagc tcaatgaaat
     1261 tgtgtcgatt agcgttattg cctccaatat catcgatgcc gatcgtatgg caacggctgc
     1321 ctttgccatg ggtgtaaaag gtatttattt tattgaacag cttggtgggt atgaagcgtg
     1381 cgctgtgacg agcgataaaa caacgattac gacaaccggc tggcagaagt atgaggaaaa
     1441 cgaatgaatt acgtagataa catgattaat cgtattacga tgtatcggtt cctaatatat
     1501 tacctgtgcg gcctgctgct cgtggcaatg ttctttggtg ctatcggtac acttgcgtac
     1561 ccaccacaga tgattctgct ctctgcgtca attactgttg gcgtatgtta cgcagtaaat
     1621 tatgtatttg ctaagatcta cgatgcaccg agtaatactg aatccgcatt aattacagga
     1681 ttgattttat cgctcatcat cactccgatg tcgtcacaga aaggactacc atttcttgtt
     1741 gctgcatcag ggctcgcgat tgcgtcaaag tatattattg caatcggaaa caaacacata
     1801 ttcaatccgg tagctattgc ggtagtgcta acggcatttg gccccggcga atcagcgagc
     1861 tggtgggttg ggtctgtcgc actgctgcca tacgtaatag ccggcggact attaatcatc
     1921 cgcaagatcc gacgtgccac aatgtttgca atttattttg tagcagcaat ttgttcggta
     1981 ataataatgg cggcggcgac aaatcacgat atagtcacta attttcaggc tacattgcag
     2041 cattcatcac tgttctttct cggtttcgtt atgcttaccg agccatggac atcaccatca
     2101 acaaagcgcc accggtatat ctatgcgaca attgtaggtt tgttgttctc gccacttata
     2161 caaatcgctg gtatttactc aaccccagaa ctcgcgttaa tactgggtaa tctctacgct
     2221 ttccttgtaa cgccgatcgc caaaacgaaa gtgcgtataa ttcaccgcac gatgtatggt
     2281 gcgaataccg aggatatcga attagcccca gaacacccat ttttatataa accagggcaa
     2341 tatattgaaa tgacattacc gcatcacggc agcgatgcac gtggcgccag gcgctacttc
     2401 acgcttgcat catcgccgac tgaagataca ttacatctag gcgtgcgtta ctacaataac
     2461 ggtagtagtt ttaaagaaac tttacatacc agtgaaaatg cgctcatgtc atttggacaa
     2521 gttggcggtg attttactct gccaagcgat gccagcgcta gacttgcgtt tattgctggc
     2581 ggtatcggcg ttacgccgtt taggagcatg gtgaaatatt tatcagatac aaacgaccaa
     2641 cggtctgcta ttttattata tggcgagcga agtgcaagcg atgttgcata taaagagatc
     2701 ttcgaggaag cgcgcgcaaa aataggactg acaacaaaat acatcttgag cgcctcattg
     2761 cagactcaag ataatgaaat tcttggaaat attagtagag aacttatcga acgcgagatc
     2821 ccagattacg catcacgaat gttctatatt tccggttcac aatcaatggt gcaaggggta
     2881 aggcgagagc ttcgcgcaat gggagttgcg cggcgaaata ttaaagcaga ctacttcttc
     2941 ggctacgcat agtaagcata tgtagtatga ttaaagtatg gatatattca ttgtattttg
     3001 tgcgaagtat ctcattatta tcattgggct acttgctctc ggctattggc ttacactgcc
     3061 gcgcaagcaa aagacagaag tactcatcat gggggctgtt accggcatca tagcgtttat
     3121 tctcgccaag acaggtagcg ccatgttcac agatcctcgt ccatttgtat ccgatggagt
     3181 gactccctta ttcgcctata ctgctgacaa cggcttcccc tccgaccata cattgttggg
     3241 tatgattgtg acgatggcgc tactgagcgt atctcgaaaa tgggctatca atctatttgt
     3301 cctgacggtt attgtcggcg catcacgtgt acttgcaggg gttcatcacc cagtagacat
     3361 tgttggcagc atcgtatttg gtgctcttgg tggagtagta gccgtgtatg ttacgcctaa
     3421 gattatcgac tggctcgcaa cgtcaagata cgccaaatca cttggtcttc atcacatttc
     3481 caagaagaaa aagtaacaag ccattacatt tttcgcggcc tcacacccct gtgaaagggg
     3541 tgtcttagta ttttgttaaa taaaatacct tgacaggcat agtaatatga tatacaatgc
     3601 agacatgatc gatacgaaat taacgtcaga tcttttacgc gggcacaccg acacgatgat
     3661 tctcaagtta ctcatcgatg gcgataggta cgggtatgag atcaccaagc acatcctcga
     3721 tgcctcgggt ggcgaatacg agctgaaaga agccacgatg tattccagtc tcaagcgcct
     3781 cgaaaccgat ggccgcattt cctcgtactg gggtgacgaa agtcaaggtg gtcgacgcaa
     3841 atattaccat ataaccgaaa gtggccggga agtttacgat acgaatatca ctacctggaa
     3901 cgcagccaag aaaatcttag aaaaattatt gtaaggagaa cgaaatgaac gacaagctta
     3961 tacaatttct cgataacgca tttaagccat acggcaattt cccagcacgc aaagacgtcg
     4021 agcaagagtt gctggcaaac ttaactgaaa agtatagtga cctcaagcgc gatggcaaaa
     4081 gtgacgaaga ggcataccaa ttaacaactg aatcgttcgg cgacgtatcg gaaatcatgg
     4141 agcaggtcgc tcctgaagag cacgcccgcg aaacccgcaa gcaagattta cgtaaaacaa
     4201 tcgttgacag catcaaagac actttcggca taggcgacac atctcgcttc cgcgcatcag
     4261 cgcttgaaga atcagatctt gcagatgtaa accttcgtgg tgcagatttt agtatgagcg
     4321 cgatgatgaa gagcaacttc aacggttcac agctacaaaa tgctaaattc aaagctgcag
     4381 cactgaaggg ctcaacattc actggcgctg acctcactgg cgctgaattt aacagcagtg
     4441 acatccagga gtcagacttc acaaacgcga acctagaagg cgccacactc atcagctgcg
     4501 cgcttaaggg cgcaaacttt aacagtgcca atcttacgaa taccgtattt aagcattcag
     4561 acctcagcga agtagtattt gatggtcaca tgctaaacgg tacagtattc gacggtgcag
     4621 gtcttaaaaa tacatcattc cagggcgcaa cgctcgtgaa tgtatcattc catcactcac
     4681 gggttaagta cacaattttc gacggtgcga caatggataa aacaaccaac gcattgttaa
     4741 agggcgcaaa agcaaacctt gataacgtaa cagtggagta acattcagtg agtacaatta
     4801 cggtaaaaaa cttaactaaa gcctacggca aacagcaagt gctcaagggc gtagacttta
     4861 ctgtcgaaga gggtaagatt ttcgctcttc t
//
LOCUS       DATIIX010000091         1709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_78811, whole genome shotgun sequence.
ACCESSION   DATIIX010000091 DATIIX010000000
VERSION     DATIIX010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78811"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VLF64_02575"
     CDS             <1..357
                     /locus_tag="VLF64_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HSW90859.1"
                     /translation="PDQTAKVAGDDGMYISGQGSSHQVAPDLAKHYSLKSTKIQSDVT
                     AISNAVKGGSYVIMSGTGSLPFTSSGHYILIRGVTADGKWKIADSAHKDTNNTAFSPS
                     TILSMASEDSMYVISK"
     gene            369..911
                     /locus_tag="VLF64_02580"
     CDS             369..911
                     /locus_tag="VLF64_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90860.1"
                     /translation="MDIKKRNLLIIFGFIMLSALSGLAAYGFYNNNRSTTNGATVKPT
                     STQKVDPVTKQVIDDGSDQTPETYGQNPAKPVLIGFAALLDQGITQNDLTAFQGGISN
                     YFVSAGNTYPPASVVVFSNAACGVPLSDGSVDCSYKLTVNDKTKIDGAFKTDENGHIT
                     IGLTQDGKTIYHTTIDENAS"
     gene            complement(908..>1709)
                     /gene="recF"
                     /locus_tag="VLF64_02585"
     CDS             complement(908..>1709)
                     /gene="recF"
                     /locus_tag="VLF64_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HSW90861.1"
                     /translation="QKRIATFDNRGEKKQKQFTIDDKTNKMLLGRFRYPVVLFQPDDT
                     RLIGGSPSRRRKYLDGVVAQYDTQYGSDVRRYERVLAQRNKLLKQPGITQDQLFSWDV
                     ILSNLGARIIAAREQYVARVNMQIEQYYRKIAKNNAEIHVEYFNHGEHTAQSLMTRMH
                     SSLERDRILGSTSVGPHRHDFSVILADKPSDTTASRGEVRTIILALKYIEVDVLFEYT
                     DQRPIVLLDDVFGELDEQRQKQLLTTLTDSQVIITSTEAHEASQHISL"
BASE COUNT          491 a          435 c          353 g          430 t
ORIGIN      
        1 cctgatcaaa ccgccaaggt agctggcgat gacggaatgt atataagcgg tcaaggttcg
       61 tcccaccaag tcgcacctga tcttgcaaaa cactacagcc ttaaatctac caaaatacaa
      121 tccgacgtta ctgctatttc aaatgcagtt aaaggtggat cgtacgtaat tatgtcaggc
      181 acaggatcac taccatttac tagtagcggt cactatattc tcattcgcgg tgttacagca
      241 gatggcaaat ggaagatagc cgactcagcc cacaaggaca ctaataacac agcgtttagc
      301 ccatcaacaa ttctcagtat ggcgtcagaa gatagtatgt acgtcattag taaataagcg
      361 aggtagccat ggatataaaa aaaagaaacc tactgatcat cttcggcttt atcatgcttt
      421 cagcgctctc aggacttgcg gcgtatggat tctataataa taaccggtca acaacaaatg
      481 gggctaccgt aaaaccaacc agtacgcaaa aagtcgatcc cgttactaag caggtaatcg
      541 atgacggatc tgatcaaacg ccagaaacgt acggccaaaa tcccgccaag ccagtgctta
      601 ttggcttcgc agcattactc gatcaaggca ttactcagaa cgaccttacc gcattccaag
      661 gcggcatctc gaactacttc gtgagtgccg gcaacacgta tccaccagca tcagtcgttg
      721 tattctcaaa tgctgcgtgc ggagtacccc ttagtgacgg ttcagtagac tgctcctaca
      781 aattaacagt aaacgacaaa acgaagatcg acggagcatt caaaaccgat gagaatggtc
      841 atatcacgat cggcctcacc caggacggta aaactattta ccataccaca atcgacgaaa
      901 acgctagcta aagtgagata tgttgactcg cctcatgcgc ctcagtcgaa gtaattatca
      961 cctggctatc tgtcaaagta gtcagaagtt gcttttgtcg ttgctcgtct agctccccaa
     1021 acacatcatc aagtagtacg attgggcgct gatcggtata ttcaaataac acatccactt
     1081 caatatattt caatgccaat atgatcgttc gcacctcacc tcgcgatgcg gttgtgtcag
     1141 atggcttatc ggcaagtatc actgagaaat catggcgatg cggcccaact gaggtgctac
     1201 caagaatcct atcgcgctcg agggagctgt gcattcgggt cattaatgat tgtgcggtat
     1261 gttcgccatg attaaagtat tctacatgaa tttccgcatt attcttagca atcttacggt
     1321 agtactgctc aatttgcata ttcaccctgg cgacatattg ttcgcgggct gcaataattc
     1381 gcgcccccaa attgctcaaa atcacatccc acgaaaacaa ttggtcctgg gtaatacccg
     1441 gctgcttgag taatttatta cgctgcgcca aaacgcgctc gtaccgtcgc acatcactgc
     1501 catattgcgt atcatactgc gcgaccaccc catcaaggta tttgcgccgc cttgagggcg
     1561 agccaccaat cagccgtgta tcatcaggct gaaatagaac cactgggtaa cgaaacctcc
     1621 cgagcaacat cttgtttgtc ttatcatcaa tagtgaactg tttctgtttt ttctcacccc
     1681 gattatcaaa tgttgcgata cgcttctgt
//
LOCUS       DATIIX010000092         2690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_549873, whole genome shotgun sequence.
ACCESSION   DATIIX010000092 DATIIX010000000
VERSION     DATIIX010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549873"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..1748
                     /gene="secY"
                     /locus_tag="VLF64_02590"
     CDS             342..1748
                     /gene="secY"
                     /locus_tag="VLF64_02590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HSW90862.1"
                     /translation="MNWRIVLRSLKNRDMQKRLSIVLGLLIAYRFMAHIPVPLANPTE
                     LKNIIDNVVSGSNFGGFLNLMSGGALAQISVILVGLNPFITASIITQLLTKAIPRLEE
                     LHKDGESGRRKINQWTRMITVPLAIVQSIAFIYILRQSVLAASTTGTVSTTPTEWVIS
                     VAAMTAGSILLMWVGELITEQGIGNGISLLIFAGIISQIPNMFGTLISSLFDTKSGSL
                     SVFGWFNLPVNPTIFWVVLSILLAGLVILYILVKINEAQRIITINYAKRVGGNTSYGG
                     IKSILPVKLIAAGVIPVIFAVAFLSLPAFIGQVMKAMPNGNHELAANLVKWFQAPSAT
                     TFANADWASLIYPIAYFVLVIAFTYFYTGIVFNSNEIAENLQKQGGFIEGVRPGAQTE
                     KYLTSMVNRLVFFGSLSLGLIAIMPFAADYIFAQLGLNMANISIGGTGLLIVVQVGLE
                     TLRQINSRALMVTYDDYK"
     gene            1874..2098
                     /gene="infA"
                     /locus_tag="VLF64_02595"
     CDS             1874..2098
                     /gene="infA"
                     /locus_tag="VLF64_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HSW90863.1"
                     /translation="MASQKEVIKLKGKVVEALPNTQFKVELENGLSIIAHISGKMRKH
                     YIRLVPGDTVEVELTPYDLTKGRIVFRARD"
     gene            2175..2291
                     /gene="rpmJ"
                     /locus_tag="VLF64_02600"
     CDS             2175..2291
                     /gene="rpmJ"
                     /locus_tag="VLF64_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HSW90864.1"
                     /translation="MKVRASVKKISPDDQLVRRKGRLRVINKKKPKNKQRQG"
     gene            2294..2680
                     /gene="rpsM"
                     /locus_tag="VLF64_02605"
     CDS             2294..2680
                     /gene="rpsM"
                     /locus_tag="VLF64_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HSW90865.1"
                     /translation="MARISGVVIPAEKQVQIALTYIYGIGPKHSRDILAAAKIEPTTR
                     VKDLTEAEEQKLRDIIDNDYVTEGDLGRLVANNIKRLKDINAYRGLRHKAGLPTRGQR
                     TRTNARTRKGKAVAVGGAQPKAASKT"
BASE COUNT          733 a          656 c          625 g          676 t
ORIGIN      
        1 ggatacgacc caagctccct ggtaggggag aacacgtacg aaacactgcg tcgtgtcaca
       61 ttgcacggcg acaacacgcc catcgctgtc gtcatcggca gccacaccta caaggttact
      121 acaacctcgt agccaccccc cgcgacccgc gccggcgaga caccccgtct cccgagtgtg
      181 ggtggcgggg tgttttcata taaatatgct atactagtca taatacattg gagtagccac
      241 gagaggttcc gggaaaagcc tgttagcagt cgctttcctt gactgctagt gctataatga
      301 atggtaatta atcgactgat aaatggggaa tatctaccga aatgaattgg cgaatcgtct
      361 tgcgctcact taaaaatcgc gacatgcaaa agcgtctcag cattgtgctt gggctactta
      421 ttgcataccg ctttatggca cacattccag tgccgctcgc aaatcctacc gagctaaaga
      481 atatcatcga taacgtcgta agcggcagca actttggtgg tttcctaaac cttatgagtg
      541 gtggcgcact cgcccagatt agtgtgattt tggtaggtct aaacccgttc attaccgcga
      601 gcatcatcac ccagttactc actaaggcca ttcctcgcct tgaagagctt cataaagacg
      661 gtgaatcagg ccgtcgcaag attaaccagt ggacacgtat gattaccgtg ccgctcgcta
      721 ttgttcaatc aattgccttt atttacatcc ttcgccaatc tgtacttgca gcaagtacaa
      781 cgggcacggt aagcaccacg ccaactgagt gggtaatctc tgttgcagct atgaccgctg
      841 gctcaatcct cttgatgtgg gttggtgaac ttattactga gcaaggtatc ggtaacggta
      901 tttcgctctt aatttttgcc ggtattatca gccagattcc gaatatgttt ggcaccctta
      961 ttagctcact attcgacaca aagtcaggct cgctcagcgt ctttggctgg tttaatctcc
     1021 cagttaaccc aacaatattc tgggtcgttc tcagtatcct gttggctggg ctcgtcatac
     1081 tgtatatatt ggtcaaaatt aatgaagccc aacgtatcat tacaatcaac tatgccaagc
     1141 gtgtgggcgg caacaccagc tatggcggca tcaaaagcat cctgccagtg aaactaatcg
     1201 cagccggtgt gattccggtc atcttcgccg tagcgttctt gagtctcccc gcatttattg
     1261 gccaagtaat gaaagcaatg ccaaacggca accacgagct cgcggcaaac ctcgttaaat
     1321 ggttccaagc gccatcagct acaacattcg ctaatgccga ttgggcaagt ctaatttacc
     1381 caattgcata tttcgtactt gtgattgcat tcacatattt ctacactggt atcgtattta
     1441 attcgaacga aatcgccgag aatctgcaaa agcaaggtgg atttatcgag ggtgttcgcc
     1501 caggtgcaca gacagaaaaa tatcttacaa gcatggtgaa ccgtctcgta ttcttcggtt
     1561 cgctctcgct tggccttatc gctattatgc catttgctgc agattatata ttcgcccaac
     1621 taggcctgaa tatggcgaac atctcaattg gtggtactgg acttttgatt gttgtacagg
     1681 taggtcttga aacattgcgt cagattaact cccgcgcgtt gatggtcaca tacgacgatt
     1741 acaaataaag agcactcatt gacaaggcaa agaggaatgc tatactgaat atagcttcaa
     1801 ggggcactga aaaagtggcc catgcctctt tactttacga gcattttcaa ctataatacg
     1861 ggattgtata tctatggcga gtcaaaagga agtcattaaa ctaaaaggta aggtagtgga
     1921 agcgctgcct aatactcagt ttaaggtaga actagagaat ggcctaagta ttatcgctca
     1981 cattagcggc aagatgcgca agcattatat ccgcttagta ccaggcgata ccgtggaagt
     2041 cgagttaacc ccatacgatc ttacgaaggg tagaatcgtc ttccgcgcac gagattaata
     2101 acgcagtagt atcggcccga tatcaccgat aatactcact aatgcaggtt tttcctgaag
     2161 gagtttttac gctcatgaaa gttcgtgcga gtgtcaaaaa gatcagccca gatgaccagc
     2221 tggtccgccg taaagggcgc ctccgtgtca tcaacaagaa aaaacctaag aacaagcaaa
     2281 ggcagggtta agcatggctc gaatctctgg ggttgttatt cccgcagaaa agcaagtgca
     2341 gatcgctctt acgtatattt acggtattgg gccaaagcac tctcgagaca tccttgcggc
     2401 ggctaaaatt gagccgacca ctcgggtgaa agatctcacc gaggctgaag agcagaaact
     2461 tcgtgacatc atcgacaacg attatgtcac tgaaggcgat ctcgggcgcc tggtggctaa
     2521 caatattaag cgtctaaagg acatcaatgc ctaccgtggc ctccgccaca aggcaggtct
     2581 gccaactcgt ggccagcgta ctcgtacgaa tgcacgaact cgcaagggta aagcagttgc
     2641 agtcggtggc gcacaaccta aagcagcaag taagacctag gaaaggacta
//
LOCUS       DATIIX010000093         3886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_50541, whole genome shotgun sequence.
ACCESSION   DATIIX010000093 DATIIX010000000
VERSION     DATIIX010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50541"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VLF64_02610"
     CDS             complement(<1..343)
                     /locus_tag="VLF64_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90866.1"
                     /translation="MSANKKFIILLIVIGLISSSAFFLFPAAKAIGLSYSIAVGFAIT
                     LTQAVALWYFLSSLKTFKSGLKIAYYWLAAGIVIFNIGSGVISGLAQLIPGLVVLVST
                     PYVIGAGCMYVG"
     gene            623..1234
                     /locus_tag="VLF64_02615"
     CDS             623..1234
                     /locus_tag="VLF64_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90867.1"
                     /translation="MRLAHIRQKGTFNEPVWHVLVVLTVAIVLQAALNNQLTFGFKYV
                     IIALEVVLFVILSTFFLPLAWRRIVAIALLALISVANMVSLSVVIAALIGSAQVNGHE
                     LLFSSVVIYLTNVIVFGLWYWELDNQRTNVDDFQFPQRAGETSQRPTFLDYLYVSITN
                     ASAFSPTDTLPLTHRAKYLMALQSLTSLAIVVLVTARAVNILS"
     gene            complement(1245..2399)
                     /gene="alr"
                     /locus_tag="VLF64_02620"
     CDS             complement(1245..2399)
                     /gene="alr"
                     /locus_tag="VLF64_02620"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HSW90868.1"
                     /translation="MTIRTTIDRFISSFEKDFATHNRIEVSKSALVHNFDLFAALSGK
                     QVIPVLKGNAYGHGIEQVATALKDQSLPYIAVDGYFEALRVRKVSQQPVLVMGAIKPE
                     NFAKLTYDQFAFVVQDKAAIRALGKTGKHVVVHLECNTGMNRYGAAKTELSELTSLIL
                     SYPNLRLEGVMSHLADSDGNNAETVENAVRIFDECVETVRMAGASPTMFHVAQTAGSL
                     KAKSKYATAMRLGIGLYGINPFSPGHLEHSKLVGLRPALQLISTITKTIYLDKGDQVS
                     YNYTFTTPKAMRIGVLPLGYYEGMPRTFSNAGIVKLGASYAVIVGRVCMNHTMINLDG
                     MTAKVGDEAIVFSSDGKNKNSIANAAKQHGLFTYNLLTTLSPDVRRILVD"
     gene            2579..3166
                     /locus_tag="VLF64_02625"
     CDS             2579..3166
                     /locus_tag="VLF64_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90869.1"
                     /translation="MTNMPQLHRVTRIRGELALHPNITKVVEGFFEHPERAETGKLYT
                     TAQLIVDQGDYQGRRYDRQLSDWERSMALKRMASQIFRIGPPLQLYEGSVMGRLQFSV
                     HPDDKQGLTEFREHLAEIGGIEPVPADDMLYLELSRGALAKNAATRREQRDETLGKLA
                     SMLRHPTAATHLTATNLRMVTKDMTRRLMAEPDAA"
     gene            3391..>3886
                     /locus_tag="VLF64_02630"
     CDS             3391..>3886
                     /locus_tag="VLF64_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90870.1"
                     /translation="MHDENLVTTLAQKQRAVRAIYISSYIPRRCGIATFTKDLTTAIN
                     NLNPHALSEIIAVLDDIEYSYPWEVKTRISQQNLSDYIDAARYINRSSTDIVNLQHEF
                     GLYGGEDGEYILSLIDDLERPLITTFHTVLPDPDEHKAYIMRRIIDKSAAVVAMTNES
                     LQTLI"
BASE COUNT         1000 a          885 c          950 g         1051 t
ORIGIN      
        1 tcccaacata catgcaccca gcgccaatta cataaggcgt cgaaaccaac accacaagac
       61 cggggatcaa ttgcgccaaa ccactgataa cgccactgcc tatattaaat ataactatac
      121 cagctgccag ccaataatat gctattttca ggccgctttt aaaagtctta agacttgata
      181 aaaaatacca caacgctact gcttgtgtaa gggtaattgc gaatccaaca gcgatactat
      241 aactcaagcc aattgctttt gccgcgggga ataaaaagaa ggccgaggat gaaatcaagc
      301 ctattactat cagtagtatg ataaatttct tgtttgcgct cataatctac ccggatcttc
      361 ttataccatt gacctaagca taaatgtttt gtcgtatgcg gtcaagtaaa gccatggtat
      421 tatatgtcca tattccatcc ggtacttcgc ggtacactcg agatgctgat cagctcgggt
      481 atcgaggccc tgagtgactg gacgtgcggg gcttggtgac gatatggatc tcccttcgca
      541 gccgatctga tctaggtcgt taggttttac gcattagaac caagccattt taatatattc
      601 tgagctacgt tatgataaca gtatgcggtt ggctcatata cgtcaaaaag gaactttcaa
      661 cgaaccggtg tggcatgtgc ttgttgtgct caccgtggcc atagttttac aggcagcgct
      721 caataaccag ctaacgttcg gttttaagta tgtcattatc gcactcgagg tagtcttgtt
      781 cgtgattctc agtacgtttt tcctgccact agcgtggcgg cgcatcgtgg caattgcact
      841 gttggcattg atttcagtgg ctaacatggt gtctcttagt gtcgtgatcg cagcacttat
      901 cggtagcgcg caggtgaacg ggcacgaact gctattttca agcgtggtga tttatctcac
      961 gaacgtgatt gtttttgggc tttggtattg ggaattagat aaccaacgta cgaacgtgga
     1021 tgattttcag tttccacagc gtgcgggtga aacctcccag aggcctacgt ttctggacta
     1081 tctgtatgtt tctattacga acgccagcgc ctttagcccc accgatacgc tgccgctcac
     1141 gcaccgggca aaatatctca tggcactcca gtcacttacg tcgctggcaa tcgttgtgct
     1201 cgtaacggct cgagcggtga atattcttag ttagccttac gtggtcaatc tacaagtatc
     1261 cggcggacgt ctggcgaaag tgtcgtcaag agattatagg tgaataaccc atgctgtttt
     1321 gcggcgttag cgatgctgtt tttgtttttc ccgtcagagc taaacacaat ggcttcatcg
     1381 cctacttttg ccgtcattcc gtcaaggttg atcatggtat ggttcatgca gacgcgaccg
     1441 acgatgacgg catacgacgc gccgagcttt acgatgcccg cattgctgaa tgtgcggggc
     1501 ataccctcgt agtatcccaa tggtaatacg ccaatccgca tggccttagg ggtggtgaac
     1561 gtatagttat agctgacctg gtcgccctta tctagataga tcgttttggt gattgtactt
     1621 ataagctgga gggcgggccg gaggcctact aactttgagt gctctaaatg tccaggcgaa
     1681 aacgggttga tgccgtagag gccgatacct agtcgcatgg cggtggcgta tttggacttt
     1741 gcctttaagc tgccagctgt ttgggcaaca tggaacattg ttggtgaggc gccagccatg
     1801 cggactgtct cgacacattc atcgaagatc cgaacggcgt tttctaccgt ttcggcgtta
     1861 ttgccgtcac tatcggcaag atgactcatg accccctcta gtcggagatt cggataacta
     1921 agaataagac ttgttaactc cgaaagctcg gtctttgccg caccatagcg gttcatgccg
     1981 gtattacatt caaggtgaac gacaacgtgt ttgccagttt tcccgagtgc acgtattgct
     2041 gctttgtcct gcacgacaaa ggcgaactgg tcatatgtta acttggcgaa gttctcgggc
     2101 tttattgcgc ccatgacaag cactggttgc tggctgactt tgcggactcg tagtgcttca
     2161 aagtagccgt caacggcaat gtagggcaat gattggtcct ttagtgctgt cgctacctgt
     2221 tcgatgccgt ggccgtaggc gttgcctttc aataccggga taacctgctt gcccgaaagt
     2281 gctgcgaaca agtcaaagtt atggactaga gcactttttg atacttcgat tcgattgtgg
     2341 gttgcaaagt ctttttcaaa actggagata aatcgatcga ttgtcgtgcg aatagtcata
     2401 tacttatctt acgctggtta acgcgactgt aaaagccggg tattccgacg tatgatgagt
     2461 gcgtagagga tatagtggtc gaagcgccgc tacatatgat gttggcgtgg tgcagtctat
     2521 gctcaatgac gaaataccaa cactaggcta aaacacatgt aagtgtcata attaagtcat
     2581 gacaaatatg ccgcaattgc atcgggtaac acgtatacgg ggtgagctgg cattgcaccc
     2641 aaatattaca aaggttgtcg aaggcttttt tgaacatccc gagcgtgccg aaacggggaa
     2701 actatacaca acagcccaat tgatcgttga ccaaggtgat taccaggggc gtcgttatga
     2761 ccgacaacta agcgactggg agcggtcgat ggcattaaaa cgaatggcat cgcaaatatt
     2821 tagaatcgga ccgccgttgc aattatacga agggtctgta atgggtcggc tgcagtttag
     2881 cgtccatcca gatgataagc aaggcctaac tgaatttcgt gaacacttgg cggaaatcgg
     2941 tggcatcgaa cctgttcctg ctgacgatat gctgtaccta gagctttcca ggggcgcctt
     3001 ggcaaaaaac gccgcgacac gacgtgaaca acgcgacgaa acgctgggca aacttgcctc
     3061 tatgttgcgt cacccgaccg ctgccacaca cctaacagcc acgaacttgc gtatggttac
     3121 taaagatatg actcgtcgcc tgatggccga acccgatgcg gcataagcgt tatactatat
     3181 gaaaaattgt taagttcata gttgccttcc tgcaaagcgt ctaatcaccc ttgttaaggt
     3241 aacggtttct tatagtaagt aggcgcaagg tctattcaga ttgataaaag atgaaatcat
     3301 tggggttcag catgtgacaa attctaggag gagtataata agaacagtag gcgtaggagg
     3361 tatctatttc ggtatgccga tgggagatgt atgcacgatg aaaatctagt gacaacccta
     3421 gctcaaaaac agagggcagt tagagcaatc tacatctcgt cgtatattcc acgtaggtgc
     3481 ggcattgcca cctttacaaa agacctaaca acggcaatca ataatctaaa tccccatgct
     3541 ctctccgaaa tcattgctgt attagatgat attgagtact cgtatccgtg ggaggttaag
     3601 acacgcatct cgcagcagaa tctgagtgat tatattgatg ctgcacgcta tattaaccgt
     3661 tcatctactg acattgtcaa tttgcagcat gaatttggcc tctatggagg tgaagacggg
     3721 gagtatattc tttcgctcat tgacgatctc gagcggccac ttattacaac gtttcatacg
     3781 gttttgcctg acccagatga gcataaggcc tacattatga ggcgtattat cgataaatcc
     3841 gctgcagttg tagctatgac aaatgaatct ctgcagacgc tcatta
//
LOCUS       DATIIX010000094         2609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_329872, whole genome shotgun sequence.
ACCESSION   DATIIX010000094 DATIIX010000000
VERSION     DATIIX010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329872"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1479)
                     /locus_tag="VLF64_02635"
     CDS             complement(<1..1479)
                     /locus_tag="VLF64_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSW90871.1"
                     /translation="MSEHPTDSAHHHASKLTHRERTVALTIVALAFVMDLLDNTIVNI
                     AIPSIRENLGASYATIQWLIAGYALSFAVLLITGGRMGDVFGYKKLFMWGVGGFTLAS
                     LLSGLACNSEVLIAARLLQGGMAALMVPQVMSLMQVMFKPSERGGVMGIFGALAGVAA
                     SLGPVIGGILIHFNIAGSTWRPIFLINVPVGIFALFMAAKYLPDGKSPHPLKLDLVGT
                     LLVVVSMFLFVFPLIEGRDYDWPVWCFAMMAAAVPVLGLFIWWQGVKEKKDGSPLVVP
                     ALMKTRTFVTGLIVNIIFEGAMIGFFLPFTLILQVGLGYEVIKAALTGIPTAIGISVV
                     MAVFSQKLIPKLGRYTMTLGTIIMAIGLGLIYFFVHWHGLDTQPWEFIPGLLVTGAGM
                     ALIMSPMFSVVLTDVDSRHAGSASGVMNAIQQLGGAIGTALIGVFFFGQLVTSAPASF
                     ASAEPSVRSSLADAHLPKSTENGIIKGAKACFVDTNKAKDASD"
     gene            complement(1574..1954)
                     /locus_tag="VLF64_02640"
     CDS             complement(1574..1954)
                     /locus_tag="VLF64_02640"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HSW90872.1"
                     /translation="MQLVSDDEFDQLIQRAMLELPQEYIRGLDNVAITYADEPTPEQL
                     VANHVGPNQLLLGLYEGIPQTSRFSTSYSGVLPDKITIFKRPIMIVAHDAASFFEQIK
                     RTLWHEIAHHYGLGHDRIHVLERS"
     gene            2088..2163
                     /locus_tag="VLF64_02645"
     tRNA            2088..2163
                     /locus_tag="VLF64_02645"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2121..2123,aa:Phe,seq:gaa)
     gene            2281..>2609
                     /locus_tag="VLF64_02650"
     CDS             2281..>2609
                     /locus_tag="VLF64_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90873.1"
                     /translation="MKRPERIAGAIYDVDDTLLVSQPTENPLSNLHQVARLAALHTIV
                     DKYDYCTQLRDVGPQKNFDSFAASPVHTIFGAFHTLLKTEAFSRARSTRCTHLSWSSL
                     LLKMKSTV"
BASE COUNT          729 a          656 c          609 g          615 t
ORIGIN      
        1 atcactggca tcctttgctt tgtttgtgtc aacaaaacat gcctttgcgc ccttaatgat
       61 tccgttttcg gtgctttttg gcaagtgtgc gtcggcaaga ctcgagcgga cgcttggctc
      121 agctgatgca aagcttgctg gcgcactcgt aacgagctga ccgaagaaaa atacaccgat
      181 cagtgccgta ccgattgcac caccgagctg ctgaatagca ttcatcacac cagacgcgct
      241 accggcatgc cttgagtcga catcagtaag caccactgag aacattggcg acatgataag
      301 tgccataccc gcacccgtca cgagaaggcc tgggataaat tcccacggct gcgtgtcgag
      361 cccatgccaa tgaacaaaga agtagatgag gccaaggcct attgccatga taattgtacc
      421 aagcgtcatg gtgtagcgac cgagctttgg aataagtttc tgactaaaga ctgccatcac
      481 tacagaaata ccaatcgccg ttggaatacc cgtgagggct gctttgatca cttcgtaccc
      541 aaggcctacc tgcaaaataa gggtaaacgg caggaagaag ccaatcattg caccttcgaa
      601 aatgatgttc acaatgaggc cagtcacgaa tgtacgagtt ttcataagtg ccggaacgac
      661 aagcggagag ccgtcttttt tctctttaac gccctgccac catataaaca atccaaggac
      721 aggaacggca gccgccatca ttgcaaaaca ccacactggc cagtcatagt ctcgcccttc
      781 aataagcggg aaaacgaaga ggaacatgga gacaaccaca agaagcgtgc caacgaggtc
      841 aagctttaat gggtgtggag atttgccgtc aggtaagtat ttcgcagcca taaagagggc
      901 aaagatacca acaggaacgt taattaggaa gattggacgc catgtagagc cagcaatatt
      961 gaagtggata agaataccac cgatgacagg accaagactt gccgcaacgc cggcaagggc
     1021 gccaaagata cccatcacgc cgccgcgttc acttggcttg aacattacct gcatcaagct
     1081 catcacctgc ggcaccatga gtgctgccat accaccttgc aaaagtcgcg ccgcaatcag
     1141 cacttcagag ttacaggcaa ggccgcttag caaacttgca agcgtgaacc cccctacacc
     1201 ccacataaag agtttcttgt agccgaatac atcgcccata cggcccccag taatcagtag
     1261 cacggcgaag cttagtgcat acccagcgat aagccactgg atagttgcgt aactcgcgcc
     1321 aagattctca cgaattgacg ggatcgcaat attaacaata gtgttgtcga gcaagtccat
     1381 gacaaacgcc agcgccacga tagtaagcgc gacagttcgt tcacggtgtg tcaatttact
     1441 ggcgtggtgg tgcgccgagt cagtagggtg ttccgacatg tgtctccttt tatttacggt
     1501 atcaattatt tagtatgcca actacttatt atacagatat agtaagtagt atttaatgta
     1561 aaaagtacat cgattaacta cgttcgagaa cgtggattcg atcatgccca agcccataat
     1621 ggtgggcgat ttcatgccat aaggtgcgtt tgatttgctc gaagaacgaa gccgcatcgt
     1681 gagcgacaat catgatagga cgcttgaata tagtgatttt atctggcaat acgccgctgt
     1741 agcttgttga gaaccgagag gtctgtggga taccctcgta tagaccaaga agcagttgat
     1801 ttgggccaac atggtttgca accaactgct cgggtgttgg ctcatcagca taggtaatcg
     1861 caacattatc aagacctcta atatattcct gcggcaattc gagcatcgct cgctggatta
     1921 gctggtcaaa ttcgtcatcg cttactagct gcattactta tccatcatag caacttaccc
     1981 actcgcttgt ctatttgcct cttatctagt aaaatagaac gaagtaaagc ctctaggcta
     2041 caccgtcaat gccgtatacg acgtaaatga tgcgacatca ccaatgtggc tctttagctc
     2101 agttggtaga gcagaggcct gaagagcctc gtgtcactgg ttcaagtcca gtaggagcca
     2161 ccaagaaaag taactcacga aagtgggtta tttttttata agcgacatga gagtgatacc
     2221 tttataggta acatcgactt tatttgcgag cgcatcttta ttctgccatt attggagaat
     2281 atgaaacggc cagaacgtat cgccggggct atctacgatg ttgatgacac cttgctcgtg
     2341 agccagccaa ccgagaatcc ccttagtaat cttcatcaag tcgccagact tgcagcactg
     2401 catacgatcg tcgataaata cgactactgt acgcaacttc gcgacgtagg gccgcagaaa
     2461 aatttcgaca gctttgccgc ctctccggtc catacaatct tcggcgcctt tcatactctg
     2521 ttaaaaaccg aggcattctc gcgggcgagg tcgacccgat gcacccactt atcctggagc
     2581 tcgttgctct taaaaatgaa gagtacggt
//
LOCUS       DATIIX010000095         4050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_245676, whole genome shotgun sequence.
ACCESSION   DATIIX010000095 DATIIX010000000
VERSION     DATIIX010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245676"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1946)
                     /locus_tag="VLF64_02655"
     CDS             complement(<1..1946)
                     /locus_tag="VLF64_02655"
                     /inference="COORDINATES: protein motif:HMM:NF036483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HSW90874.1"
                     /translation="MVEHTHGYIEIVGARENNLKNVSLQIPKRKITIFTGVSGSGKSS
                     IVFDTVAAEGQRLLNENFSAFVRTFLPKVKQPEADSIANLNMPIIVDQRKLGGGSSST
                     VGTVADIAPLIRRLFSRIGQPNVPQVYMFSFNNAAGMCPECKGLGSNMGLDVEAALDT
                     SLSLNEGAIQLPDYGVDGWYWKQVVAATGIDPDKRLKDYTDAEMNELLYGETRKVKLE
                     GVGINMTYEGVARRFATKYITRDLSAMSERTQKAVKPFMAMAPCPLCHGARLNQTALA
                     SKINGHNIADYNAMQIDALLEALDVIEDSSVTKLVAMLRGRLNHLNEIGLNYLSLDRT
                     TDSLSGGESQRVKLVKYLNGTLSDVMYIFDEPSVGLHPRDVHRLNELLKKLRDNGNTV
                     IVVEHDPDVIKVADHVIDVGPGAGSRGGEIMFEGTYEGLLKSDTLTGKALSQHLDLKD
                     DVRTPTGEIRIENAHANNLHNVDVSIPTGVLTAITGVAGSGKSSLINQTFVKQHPDSI
                     VIDQSPVAANSRSNVLTYTGVMDEIRKSFAKENDVDASLFSFNSKGACENCQGTGYVA
                     VEAAYIEEAKTICEICEGKRYKEEVLQYQWHGKNIVEVLDLTIADAVSFFENPAVTKQ
                     LQALFDVGLDYLTLGQPLSTLSGG"
     gene            complement(1950..2306)
                     /locus_tag="VLF64_02660"
     CDS             complement(1950..2306)
                     /locus_tag="VLF64_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSW90875.1"
                     /translation="MKLLGLRTLIYPSNNLEADTQWWADALGVEPYFDQPFYVGFNVG
                     GYELGLDPNGDMADGPRTYFGVDDINEAVDHFIASGCEIYEPVTEAGGDIMVATVKRP
                     NGQLLGLIYNPHFKEV"
     gene            complement(2425..3045)
                     /locus_tag="VLF64_02665"
     CDS             complement(2425..3045)
                     /locus_tag="VLF64_02665"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HSW90876.1"
                     /translation="MPNSVTAVDAQRELRMFSRPERVESTARFFKAYPGGYSEGDVFL
                     ACGVPATRDVAKHFAAMAFTELDKLIASKWHDDRLLALIILVNQYKKGDPAERAAIYN
                     FYLAHKAYVNNWDLVDSSAEFIVGPELQDNPDKFAVLKPLALSEDVWDRRIAMIATFA
                     YIKQGEADDALVIAEILLHDTHDLIQKAVGWMLREIGKRVNRDTLL"
     gene            complement(3035..3220)
                     /locus_tag="VLF64_02670"
     CDS             complement(3035..3220)
                     /locus_tag="VLF64_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90877.1"
                     /translation="MDSREAVQGFADTAKQNGGDWHMAALNEGMDGMFGLEVQDPDGN
                     TLEPMWMDMSFDPHHAE"
     gene            complement(3232..3456)
                     /locus_tag="VLF64_02675"
     CDS             complement(3232..3456)
                     /locus_tag="VLF64_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90878.1"
                     /translation="MSKQVFINFPVNDLEKSVKFYEALGFTKNEMFSDERAAALQWSD
                     DIVFMLLTREFYGQFLKDRLLRTRRILVAR"
     gene            3593..4033
                     /locus_tag="VLF64_02680"
     CDS             3593..4033
                     /locus_tag="VLF64_02680"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HSW90879.1"
                     /translation="MDRRITTRGIIYKNGKIFAQKLKRGEGATDYWCTPGGGLDPHES
                     LQDGLVREMIEETGVSPEIGKLLYIQQYREENGREYLEFFYHIINVDDYETIDLASTT
                     HGEIEVAEYGFVNPSTTIVLPKFLGEVDIARDIKAGLVQEFNYL"
BASE COUNT         1118 a         1082 c          830 g         1020 t
ORIGIN      
        1 tcaccgccag atagcgtgct cagtggctgc ccaagggtaa ggtaatcaag cccaacatca
       61 aacagcgcct gcaattgctt tgtcacggca ggattttcaa agaagctcac cgcatccgca
      121 atcgtcaaat caagcacctc aacgatattt ttgccatgcc attggtactg caagacttct
      181 tctttgtatc gtttgccttc gcaaatctca caaattgttt tcgcctcctc tatgtacgct
      241 gcctcgacag caacatagcc agtgccttga cagttttcac aggcgccctt tgaattaaag
      301 ctaaacaggc tcgcatcaac gtcgttttct ttcgcaaaac tcttacgaat ctcatccatg
      361 acacccgtgt acgtaagtac attagagcga ctgttcgcag cgaccgggga ctgatcaatc
      421 acaatcgaat ccgggtgctg ctttacgaat gtttggttaa tcaacgagct tttacccgat
      481 cctgccactc cagttatagc ggtaagcacg ccagttggaa tagagacatc cacattatgc
      541 agattgttag cgtgcgcatt ttcgatgcga atttcacccg ttggtgtgcg aacgtcatcc
      601 tttaaatcaa ggtgctggct cagcgcctta cccgtaagcg tgtcagattt caacaagcct
      661 tcgtaagtgc cctcaaacat aatctcaccg cctcgcgacc ccgccccggg gccaacatca
      721 ataacatgat cggcaacctt aatcacatca gggtcgtgtt caaccacaat caccgtattg
      781 ccattatcgc gcagtttttt aagaagctca tttagacgat gaacatcgcg tgggtgtagc
      841 cccacacttg gctcatcaaa aatgtacatt acgtcgctca atgtgccgtt aagatatttc
      901 acaagcttca cacgctgcga ctcgccacca gatagcgaat cggtcgtacg atctagactg
      961 agatagttaa gtccaatttc attcaggtga ttcaatcggc cgcgaagcat cgcaacaagc
     1021 ttcgtaactg aggaatcttc aatgacgtca agcgcctcaa gcaaggcatc aatctgcatc
     1081 gcattgtaat cagcaatatt atggccgtta attttgctcg ccagcgccgt ttgattcagc
     1141 cgtgcgccat ggcaaagcgg gcaaggggcc attgccataa atggcttcac ggccttttgc
     1201 gtgcgctccg acatcgctga aagatcgcgc gtaatatact tagtcgcaaa gcggcgcgca
     1261 acaccctcgt aggtcatgtt gataccaaca ccttcgagct tcactttacg tgtctcacca
     1321 tacagcaatt cgttcatctc ggcatcggtg taatctttca gcctcttatc tgggtcaatc
     1381 ccggtggctg caactacttg cttccagtac caaccgtcaa ctccataatc aggcaattga
     1441 atcgcaccct cattcagcga cagagaagta tcaagtgctg cttcgacatc aagccccata
     1501 ttgctgccaa gccccttaca ttcgggacac atacctgcgg cattattaaa cgaaaacata
     1561 tacacttgtg gcacatttgg ctgaccaata cgtgaaaaca agcgacgaat cagcggcgca
     1621 atatcagcga ccgtcccgac agtagagcta gagccgccac caagcttgcg ctggtctaca
     1681 ataatcggca tgttcaggtt tgcaatacta tcagcctccg gttgctttac ctttggcaaa
     1741 aaggtgcgca caaacgcgct gaaattttcg ttcagcaaac gctggccttc tgcagcaacc
     1801 gtatcaaaca caattgacga tttgccagat ccggacacac cagtaaaaat cgtgatctta
     1861 cgttttggta tttgcaggct cacattttta aggttattct cacgcgcgcc aacaatttca
     1921 atatagccgt gggtatgttc taccattagt taaacttcct taaagtgagg gttataaatt
     1981 aatccgagta actgaccgtt tggtctcttc acagtcgcga ccataatatc accaccagcc
     2041 tcagtcactg gctcgtaaat ttcacagcca ctcgcaataa aatggtcaac agcctcattt
     2101 atatcatcta caccaaagta tgtgcgcgga ccatctgcca tgtcaccatt cggatcaaga
     2161 ccgagttcat acccaccaac attaaacccg acatagaacg gctggtcaaa gtatggctca
     2221 acaccgagcg catccgccca ccattgcgta tcagcttcta aattgtttga tggatagata
     2281 agtgtacgaa ggcctaaaag tttcatacat ctatcgtatc aaacttcgct gccgcatata
     2341 atgcactcgt tgttcttggg gcaagtgttc aatcgcatac cgtagcgttg tgcgcggcat
     2401 agtcgtcgca tgagtatcca gaaatcacag cagggtatcg cggtttactc gcttcccaat
     2461 ttcacgcagc atccaaccaa cagccttttg aatcaaatca tgagtatcgt gcaaaagtat
     2521 ctcggcaatc acgagcgcat catccgcctc gccctgttta atgtaggcaa atgtagcgat
     2581 catggcgatc cggcggtccc aaacgtcctc cgataaggca agaggcttca atacggcaaa
     2641 cttatctgga ttgtcctgta attctggccc aacaataaac tccgcactac tatcaactaa
     2701 atcccagttg ttcacatatg ccttatgcgc aagatagaaa ttataaattg cggcacgctc
     2761 agcagggtca ccctttttat attgattcac caatataatc agcgccaaca atcgatcatc
     2821 atgccatttg ctcgcaataa gcttatcgag ctcggtaaac gccatcgcag caaaatgctt
     2881 cgctacatca cgcgtcgccg gtacgccaca tgctaaaaag acatcgccct cagagtatcc
     2941 gccaggatat gccttaaaaa atcttgcggt cgattccaca cgctcggggc gagaaaacat
     3001 acgtagttcg cgctgtgcat ccactgctgt cacgctattc ggcatggtgc ggatcaaaac
     3061 tcatatccat ccacattggc tcaagtgtat tgccgtcagg gtcttgcacc tcaaggccaa
     3121 acataccatc catgccttcg tttagtgcgg ccatatgcca atcaccacca ttttgctttg
     3181 cagtatcagc aaagccttgc accgcctcgc gactatccat gctcagcgcc actaacgcgc
     3241 cactagtatc ctgcgtgttc gcaacagtct atccttcagg aactggccat aaaattcacg
     3301 ggtcagcagc ataaatacaa tatcatccga ccattgtagg gcggcggcgc gctcgtcgct
     3361 gaacatctcg ttcttcgtaa agccaagcgc ttcataaaat ttcactgatt tctctaaatc
     3421 attaactggg aagttaataa aaacttgttt actcatagca ccccctccat attacttact
     3481 tttagtatat actattacat attatgatcc cacaggatcc actatgcgca tacgtttgga
     3541 cgggccaaac cgctgacgct tcttgattca ataatttata aaggagactc taatggatcg
     3601 acgcattaca acacgaggca ttatctacaa aaatggtaaa attttcgcgc aaaagctcaa
     3661 aagaggtgag ggcgcaaccg actattggtg tacgccaggt ggcgggctgg acccacacga
     3721 atcgctacaa gatgggttag tgcgcgaaat gatcgaagaa acaggtgtat cgccagaaat
     3781 cggtaagctc ctatatatac agcagtatag ggaagaaaac ggccgcgaat accttgagtt
     3841 tttctaccat atcattaatg ttgatgatta cgaaacgatt gatctcgcca gcactaccca
     3901 cggtgaaata gaggttgccg aatacggttt tgtcaaccca agcacaacaa tagtcttacc
     3961 aaaattcctc ggggaagtag atatcgcgag agatatcaag gcggggctag tacaagaatt
     4021 taattacttg tagcagcaaa attaaagagc
//
LOCUS       DATIIX010000096         3492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_524607, whole genome shotgun sequence.
ACCESSION   DATIIX010000096 DATIIX010000000
VERSION     DATIIX010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524607"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VLF64_02685"
     CDS             complement(<1..440)
                     /locus_tag="VLF64_02685"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90880.1"
                     /translation="MQPVTRLYTQFIPHHYDIHWDLTKAKEDRTILGKVTISGEQKSA
                     SSIRLHAHELTVRSVIVNSVQVKDFKLDPDADELIIPHHTPGQVTLAIEFSLDLTDAM
                     HGLYPCYFVHDGVRQELYATQFESHHAREAFPCIDEPEAKATFSV"
     gene            464..1153
                     /locus_tag="VLF64_02690"
     CDS             464..1153
                     /locus_tag="VLF64_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HSW90881.1"
                     /translation="MRSQRVVIMFVAVLTALFWMAMQQLQMTVPKSILEVKSTTPADS
                     KSALSVLDGLAVKGRAPKTGYARTQFGSGWASSNGCDTRERILQRDLLNAVTSDGCKV
                     ISGLLHDPYTGKDITFTRGIDTSSAVQIDHVVALGNAWQTGAQQLTTTQRQQLANDPL
                     ELLAVDGPANQQKSDGDTATWLPANKPFRCQYVARQITVKVKYTLWVTAAEKEAMQRV
                     LTTCPHQPIAS"
     gene            1375..1632
                     /locus_tag="VLF64_02695"
     CDS             1375..1632
                     /locus_tag="VLF64_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90882.1"
                     /translation="MNDNNHASLPIDANLLAVADALVEAGNLEALHALRGALTKLILG
                     DVTSSSLVKINEYVTEALSLLEDGRTPEEVKVSTIRELIDK"
     gene            1872..2999
                     /gene="nusA"
                     /locus_tag="VLF64_02700"
     CDS             1872..2999
                     /gene="nusA"
                     /locus_tag="VLF64_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HSW90883.1"
                     /translation="MDDLNIKQLTLAVRSIADEKNLPEETVMDVIEQAIAAAWRRDNG
                     ERDQNVRAELNMVDGTAKVFVQREVVEDVVNDLTEISLEDAQKVKSDAKLEDIIEDKF
                     EVTSFGRVAAQTAKQVVLQRLREAEREVVLAEFEDKIGTVVTGIVQRVEPRVVRIELG
                     KATGILPGSEQIQGEFYSPGQRIKVFIKDIERDNRGPQLILSRGNEQFIEFLFRQEVP
                     EMETGAVEIKGIAREAGRRTKLAVTSTVPGVDPVGTFVGGHGTRVNAVMNEIGDQEKI
                     DIITFDENLDTYIRNALSPAEVVKVEIDKDEKRAKVFVTEDQQSIAIGRGGQNVRLAS
                     RLTGYELDIETATAKQTEKKPKKNIEDDLFSAIEGQNEEDK"
     gene            3150..>3492
                     /locus_tag="VLF64_02705"
     CDS             3150..>3492
                     /locus_tag="VLF64_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HSW90884.1"
                     /translation="MPEDFADFISHLTDNARMSVQYADAIARGNGSAYIGTEHLLLGV
                     LAQGSSMGAKILADAGVTLQQAELALDIKPAKVVVSTGTVGLSETALLTLRMAWETAK
                     EFNQDYLGTEHI"
BASE COUNT         1014 a          752 c          889 g          837 t
ORIGIN      
        1 accgaaaaag tcgcctttgc ctcaggttcg tcaatacaag ggaaagcttc acgggcatgg
       61 tgactctcga actgtgttgc gtagagctct tggcgaacgc cgtcatgtac gaaatagcag
      121 gggtagagtc cgtgcattgc atcagtaagg tcaaggctga attcaatggc taaggtaact
      181 tggccagggg tatggtgtgg aatgataagc tcgtccgcat ctggatccag tttgaaatcc
      241 ttaacttgga cactatttac gatgacacta cgtacagtga gttcatgcgc atgtaagcgg
      301 atgctactgg cacttttctg ctcaccgcta atggtgactt tgccaagtat cgtgcgatct
      361 tcttttgctt ttgtgagatc ccaatggatg tcatagtgat gtggaataaa ctgagtataa
      421 agacgggtaa caggttgcat accatccagt atatactaaa gatatgcgga gtcagcgagt
      481 agtaatcatg ttcgtggcag tactaactgc gcttttttgg atggccatgc agcaattaca
      541 gatgacagta ccaaaatcta tccttgaggt aaaaagtacg acaccagcag acagcaaaag
      601 cgctctctca gtgcttgatg gccttgccgt gaaagggcgt gcgccaaaaa ctggatatgc
      661 acggacgcag tttggtagcg gctgggcatc aagtaatggt tgtgatacac gtgaacgaat
      721 tttgcagaga gatttgctaa atgcggtcac cagtgacggc tgtaaagtaa taagcggact
      781 gctccatgac ccttataccg gcaaagatat tacatttacg cgaggcatcg atacgagtag
      841 cgcggtgcaa attgaccacg tagtcgcatt gggtaatgcc tggcaaactg gcgcacaaca
      901 attgacgact acccagcgac agcaacttgc gaacgatcca cttgaacttt tagcagtaga
      961 tggcccggca aatcaacaaa aatcagatgg cgatacagca acatggttac cagcaaacaa
     1021 accattccgt tgccaatatg tagcgaggca gattactgtg aaggtgaaat atacgctatg
     1081 ggtaactgcg gcagaaaaag aggcgatgca gcgagtgctc acaacatgtc cccatcagcc
     1141 tatagcaagt tagacgccac atgttaacaa aaggttaaat agcgcaagct tattcttgtc
     1201 agggttttga ggatcctaca tactctatct aagtgagaac gaatgtggaa gtcattcgtg
     1261 ttttgtcgtg ttgcgttgat attcattctt gtgaagtatt agctacatac atctcgcacc
     1321 caaagtgtgt cttgggtagt ctttatacag ctaatttatc gaaggagtta tggaatgaat
     1381 gataataacc atgccagctt accaatagat gctaatttac ttgctgtagc cgatgcgctc
     1441 gtagaagcag ggaacctcga ggctttacat gctttgcggg gcgcactcac aaaactaata
     1501 cttggcgacg taacaagttc gagtctcgtt aagataaatg aatacgtcac agaggccctc
     1561 agtctacttg aggacggaag aacacctgaa gaagtgaaag tctcgacaat tcgagaattg
     1621 atcgacaaat agagacaccc ccaagccgac ttactcctag gacaatgtct ccattgacaa
     1681 atttgcagtg gtttgatatc ataaaaaagt agtaatccgg ccggcaggtc ggcattttta
     1741 ataattaagc atagtcaacc tctagagcgt cgctgcaatc acagtgactt gagggagagg
     1801 gcattgagtg acgaattcat ttcggaatca gtgcggaggg tgaagccctc ctaaagagaa
     1861 aggatagtta tatggacgac ttgaatatta agcagttgac acttgcggtt cgctcaattg
     1921 ctgacgaaaa gaacctccca gaagaaacag ttatggacgt cattgagcag gcaattgctg
     1981 ctgcatggcg tcgcgataac ggtgagcgcg accagaacgt tcgtgcagaa cttaacatgg
     2041 ttgacggtac cgctaaggta ttcgtacagc gtgaagtagt tgaagatgtc gtaaatgacc
     2101 ttactgaaat cagccttgaa gatgcgcaga aagtgaaatc cgatgcgaag ctcgaagaca
     2161 ttattgaaga taagttcgaa gtaacaagtt tcggccgcgt agccgcacag acagctaagc
     2221 aagttgtact acagcgcctt cgcgaagcag agcgtgaagt cgtacttgct gagttcgaag
     2281 acaaaatcgg cactgttgta acaggtattg tacagcgcgt tgagccacgt gtcgtgcgca
     2341 ttgagctcgg caaagctacc ggtatcctgc cagggagcga gcaaatccag ggtgaattct
     2401 acagtccagg ccagcgcatt aaggtattta tcaaggatat cgagcgcgat aaccgtggcc
     2461 cgcagctcat cttaagccgt ggcaatgaac aatttattga attcctcttc cgtcaggaag
     2521 tccctgaaat ggaaactggt gccgtcgaaa tcaagggtat tgcccgtgaa gccggtcgtc
     2581 gtactaagct tgcagtaacg agtactgtgc ctggtgtcga cccggtcggt acattcgttg
     2641 gtggtcatgg gacgcgtgtg aatgcagtca tgaacgaaat cggcgatcag gaaaagatcg
     2701 acattattac atttgacgaa aacctcgaca cgtacatccg caacgccctc agcccagctg
     2761 aagtggtgaa ggttgaaatc gacaaagacg aaaagcgtgc caaggtattc gtcactgaag
     2821 accaacaaag catcgcaatt ggccgtggtg gccagaacgt ccgtctcgcg agtcgtctta
     2881 ctggctatga actcgacatt gagaccgcta cagcaaagca gactgaaaag aagccaaaga
     2941 agaacatcga agacgatctc tttagcgcta ttgaaggtca gaacgaagaa gacaaatagt
     3001 cctctagaag ttttacaaaa tgccccacct gtcagttggc ggggtatttt gtttacctaa
     3061 aataaagtta gcactctgag ttgacgagtg ctaattatgt gcagtatact ggacgtatag
     3121 ggccaataaa ccctgaatgg agagcattta tgccagaaga ttttgcagat tttatttcac
     3181 acttaacaga taatgcacga atgagcgtcc aatacgctga tgctattgca cgtggcaatg
     3241 gtagcgcata cataggtacc gaacatctat tactcggtgt ccttgcgcag ggctcaagta
     3301 tgggcgccaa gatactcgcc gatgctggcg tcacgttaca gcaagctgag ctcgcacttg
     3361 atatcaagcc ggcaaaagtc gtcgtgagta ccggtaccgt tgggttgagc gaaacggcgt
     3421 tattgacgtt gcgtatggct tgggaaaccg ccaaagaatt taatcaagac tacttaggta
     3481 ccgagcatat cc
//
LOCUS       DATIIX010000097         7723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_80010, whole genome shotgun sequence.
ACCESSION   DATIIX010000097 DATIIX010000000
VERSION     DATIIX010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7723
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80010"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..574)
                     /locus_tag="VLF64_02710"
     CDS             complement(188..574)
                     /locus_tag="VLF64_02710"
                     /inference="COORDINATES: protein motif:HMM:NF018826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HSW90885.1"
                     /translation="MFNLQQINDIHDKFGKSETLGLYLKELRLIGVNLQESYLSDGHS
                     VYYGNNGEKLESPSIHKTYIISDNSDKNSFMRTLKLAEEGKLNYFDMSEQFAHSGVEK
                     WVFDTDQMTITYYDKIGDTLLSEDIK"
     gene            complement(631..707)
                     /locus_tag="VLF64_02715"
     tRNA            complement(631..707)
                     /locus_tag="VLF64_02715"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(671..673),aa:Lys,seq:ttt)
     gene            827..1012
                     /locus_tag="VLF64_02720"
     CDS             827..1012
                     /locus_tag="VLF64_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90886.1"
                     /translation="MYWSRRRIHKGFSGFSASIMGEVIHIQRPLPGDVALDVRDGFAS
                     DIRQAIAEPVAGLGLWT"
     gene            1028..1288
                     /locus_tag="VLF64_02725"
                     /pseudo
     CDS             1028..1288
                     /locus_tag="VLF64_02725"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
     gene            1345..2070
                     /locus_tag="VLF64_02730"
     CDS             1345..2070
                     /locus_tag="VLF64_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HSW90887.1"
                     /translation="MSGHSKWATTHRQKAIVDAKRGAVFTKLGNMIAIAARGGIDPVM
                     NSSLALAIEKAKAANMPLANIQRAIDRVADKNAAALEEIVYEGYGPGGVGIIVEVATD
                     NKNRTYPEVRTALTKNGGTMADAGSVLFQFTRRGVIRVAATGEDALLTVLDAGAEDAI
                     EESSGLTVYTDMKDLAKVRTAIVEAGLAVEDAELQYVANNDVPVNDPETARKLLKVLD
                     ALEDLDDVVNVHTNGEITAEVDN"
     gene            2305..2787
                     /gene="ruvC"
                     /locus_tag="VLF64_02735"
     CDS             2305..2787
                     /gene="ruvC"
                     /locus_tag="VLF64_02735"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HSW90888.1"
                     /translation="MRIIGIDPGTGILGFGVIDANRGKTKLVTAGVITTPAHTPLPDR
                     LEEIYNGLTEIIDETKPTVMAIEKLFFNQNVTTAMSVSHARGVAMLTGQQAKLRIEEY
                     TPLQIKQTMTGYGRADKKQIQEMVRMQLGLTEVPKPDDCADALAAAIMCAFTLRTEQV
                     "
     gene            2797..5205
                     /locus_tag="VLF64_02740"
     CDS             2797..5205
                     /locus_tag="VLF64_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HSW90889.1"
                     /translation="MVKRKQPVRMNRYTNLAHNKPKAHAATAPKKRGLIGRFIGLSRK
                     RKIIVIAIPIVAALILIPLITYIILANDIDNPERLMNRNNTGIVLEDMNGKPFYSVGK
                     AEHRDQIPLDQISPNMKNALVASEDHDFYKHGGFSFSGIFRALFTGVGGGSTLTQQLV
                     KNTLLSDQHSYVRKYQELFMSIAVEQHYSKDQILDMYLNSVFFGENAFGIEDAAKLYF
                     GKVPKDLDLAESAMLVGVLPAPNAYSPISGNATYAKERQTTVLARMVTNGYITEEQKT
                     AALAETLVYASTAQSINSVAPHFAQMVIDELSKKYGYETVMRSGYQVKTTLDLTAQQN
                     LLDSVGAGMAHINAMGGSNASGIVIDPASGGIRALVGSANYDDPSWGKVNMVTTARQP
                     GSSFKPIYYSNALADGTITPATVLQDKVIDFGGGYVPRDADHNEASRGQVTTRQALDW
                     SLNIPSIHVMQSYGVSKTIQAAKNLGITTIDASKNYGLTLALGSAEVPLEQMAHAYAA
                     FANQGQQYDVSTINQVEDKYGKNIFKSLPISKQVISKEGAYLLSSILSDNKTRSHIFG
                     SSLTVSGHTVAVKTGTTNDDRDAWTIGYNPQYVVGVWVGNNDNTVMASGGSDMAGPIW
                     RGTMTKLLSGKKDVPFVVPSGITQRQVCSGSGGLADKAGPNTYSEYFMPGALPTNSCQ
                     AAPIMMSVCDLQTGKVTSIDESAFDSTKQSKDTANCKAPTEQACNLSTGKVETINTSD
                     FDGTKYSTDTANCKTSSNNSDGTHSDTVTACDMQTGQWVQIPQSQVDGTRYMTGMATC
                     QVSH"
     gene            5224..5796
                     /gene="ruvA"
                     /locus_tag="VLF64_02745"
     CDS             5224..5796
                     /gene="ruvA"
                     /locus_tag="VLF64_02745"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HSW90890.1"
                     /translation="MIAHIAGVVAEKFGGSVIVDVVGVGYEINVTALDYEAVTLDQQV
                     KFYTYHHIREQSQELFGFSSLAAKKLFEMLITVQGVGPKAALAILSLGEAEHVRNAIA
                     NADSMYLAKASGVGKKTAERVVVDLSDKVGMPVVYKQEQAAQTELNTSDEALEALIAL
                     GYNLADATKALDGIDTGLPTAKRVTEALRG"
     gene            5817..6818
                     /gene="ruvB"
                     /locus_tag="VLF64_02750"
     CDS             5817..6818
                     /gene="ruvB"
                     /locus_tag="VLF64_02750"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HSW90891.1"
                     /translation="MAIERIVDTSTHDEDSDEQIIEITLRPQNFDDYIGQERLKKNLR
                     LAIDAAKKRGEPIDHVLLYGPPGLGKTTMASVIANEMGAGLRITSGPAIERAGDLASI
                     LTNLQDGDILFIDEIHRLSRVVEEVLYSAMEDFKLDIVIGKGPAARSIRLDLPKFTVI
                     GATTRTGSLAAPLRDRFGHIYRLEFYTPDEIGKIITRSAKILESRIDAESAALLSTRA
                     RLTPRIANRLFKRVRDFADVNGDGIIDTTMTHGALQMMEIDDLGLDPADRNLLESIIE
                     HYGDRPVGLNTIAALTGDEMSTIEDFYEPYLIQIGFIERTPRGRQVTQKAKRHLQKQ"
     gene            6836..7588
                     /locus_tag="VLF64_02755"
     CDS             6836..7588
                     /locus_tag="VLF64_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90892.1"
                     /translation="MKKDDQTNDKPVAYDTDGKPLFATPQQPTEPEVQRQIVHIARST
                     DPIRQEVSPEIQKKHNESMRLYPNLNLSDAEYVISSVRRHPIGLFAPVFVTGLLVAMI
                     ASFLANYYNIMQLLHIVNIPKFGTVLLLGVLAIILVLMGGYIAIWVYLSNQFFLTNES
                     VIQEIQLTLFSRREQTVSLLNIEDASFLQTGILQTMFNYGSIRLSTEGEETTYRFSYV
                     ANPRAEIAQLNNAVEAFKNGRPVEVVEANEKN"
BASE COUNT         2207 a         1670 c         1933 g         1913 t
ORIGIN      
        1 gatgcttacg tatggcgcgt ttagtaatgc agtagtactc gacaatggga gtaagcaata
       61 cgtactcgag attgtgaaat atcaaaacat tgttataacg gtgtgcgttt tactggcgat
      121 tatagaagtg ttattttatc ggaaaccccg tgggcatgag cataaaactc atcgagagta
      181 gcatgtgtta ttttatgtct tcgctcagca atgtatctcc tattttatcg tagtatgtaa
      241 ttgtcatttg atctgtgtca aatacccatt tctcgacacc actatgtgca aattgctctg
      301 acatatcaaa gtagtttagt tttccctctt cggcaagttt cagtgttctc atgaaagaat
      361 ttttgtcgct attatcagag ataatgtatg tcttgtgtat tgatggcgat tctagttttt
      421 caccgttatt accataatat accgagtggc catcagataa atatgactct tgtaaattta
      481 ctccgatcaa acgaagttct ttaagatata atccaagggt ttcagactta ccaaacttat
      541 cgtgtatatc attaatttgc tgtaagttaa acattatgcc tatcatatca aaaataaaaa
      601 gaccattgaa ggtcttttgg cgatcaaact tggagggccc agtgaggctt gaactcacga
      661 caccctgctt aaaaggcagg tgctctaacc ggctgagcta tgggcccata gctgcttgtc
      721 ataaagcttc taaaactata ccacctcacc tctgatactg tcaagatagt taacgcgaat
      781 gctttgtagc tgtacgccgc ttttcgctct atgataccat caggcaatgt actggtcgcg
      841 caggcgaatc cataaaggat tcagtggatt cagcgcctcg attatggggg aagtgataca
      901 catccagcgc ccgctacctg gcgacgtagc gttagatgtg cgtgatggat ttgcgagtga
      961 tattcgccag gcgattgccg agcccgtcgc tggattaggg ctgtggacgt aatttcaccc
     1021 agccacactc cttgcggtag cggcctctgc gacggtgcta atgaatccta gctatgtgtg
     1081 gggagatcta ggatggctgc tgagcttcgc ggcatttgca ggcgtgatga ttattgcatc
     1141 aattgtgacc gcctattttt ttggtgaaga aaacgtgcca tttctcggcc agctactcat
     1201 tgaaacaata gccactcaag tcgctacgct acccattatg gtcatggcgt ttcagcaact
     1261 tagcatgatt gcgccagtcg tagaataacg ggggtgaatg tggtatacta cgattagatt
     1321 gatcaggaat attaggagat ttctatgtca ggacacagta agtgggcgac gactcatcgc
     1381 cagaaagcaa tcgttgatgc aaagcgcggc gcagtattta ccaaacttgg taatatgatt
     1441 gcaattgcag cgcgtggtgg tattgaccct gttatgaact caagtcttgc gcttgcgatt
     1501 gaaaaagcca aggcagcaaa catgcctctc gcgaacatcc agcgggcaat tgatcgtgta
     1561 gccgataaaa acgcagcagc acttgaagaa attgtctacg aaggctacgg cccaggtggt
     1621 gtaggcatca tcgttgaagt tgcaaccgac aataagaacc gcacctaccc agaagtccgt
     1681 acggcactca caaaaaacgg tggcacaatg gcagatgctg gtagcgtgct cttccagttt
     1741 actcgcaggg gagtgatccg cgttgcagca actggcgaag atgcattact aactgtgctc
     1801 gatgccggcg cagaggatgc gatcgaagaa agcagtgggc taacagtata tactgacatg
     1861 aaagatcttg cgaaagttcg tactgcgatt gttgaggccg gcctagctgt tgaggatgca
     1921 gagctacaat atgttgcaaa caatgatgtg ccagttaatg atccagaaac agcccgcaag
     1981 ctgctgaaag tgcttgacgc ccttgaagat cttgatgatg ttgtgaacgt acataccaac
     2041 ggtgaaatca ctgccgaagt tgacaattag ccacaaagtg ccactattta accagttttt
     2101 tattgacccc cgccgcactt atctataggg tgtatattta aggtatacat aagttttagg
     2161 ggtaaaagtg aagtggctaa acataattgt tgtgtagcac ttagctacgg tgtttgggag
     2221 tggcgtgaag aaattggtac agctggtggc caatttcttc gaatactaag ccgccgtaaa
     2281 gaatagactg gtatactagt acaaatgaga attatcggaa tcgacccagg aacgggtatt
     2341 ttgggatttg gcgtgattga cgctaatagg gggaaaacca agctagtgac ggctggggtg
     2401 attacaacac ccgcacacac gccgctgcca gaccgtcttg aagagatcta taatggactt
     2461 actgaaatca ttgacgaaac aaaaccaact gtgatggcga tcgaaaagct tttttttaat
     2521 caaaacgtaa ctaccgctat gagtgtatcg catgcgcgcg gcgtggcaat gctgactggg
     2581 cagcaagcaa agttgcggat agaagagtac acaccactgc agattaaaca aaccatgaca
     2641 ggctacggca gagcagataa aaagcagatt caagagatgg tccgtatgca gctcggtcta
     2701 acggaagtac caaagccgga tgactgcgcc gacgcgctgg cggctgcaat aatgtgcgcg
     2761 tttacactgc gtaccgaaca ggtgtaaaat gatcctatgg taaaacgtaa gcagccggtg
     2821 cggatgaacc gctacacaaa ccttgcgcat aataaaccta aggcacatgc ggctacggca
     2881 cccaagaaaa gggggctgat cggccggttc attggactat cccgtaaaag gaagattatt
     2941 gtcattgcaa tacctatcgt tgccgcttta atattaatac ccttaatcac gtatataata
     3001 cttgcgaacg atatcgataa ccctgagcgt ctgatgaacc gaaataacac aggcatcgta
     3061 ctagaagata tgaatggcaa accattttat agtgttggca aggctgaaca tcgcgaccag
     3121 atcccgcttg accagatatc acctaatatg aaaaacgcac tcgttgcaag cgaagatcat
     3181 gatttttata agcatggagg gttcagtttt tcaggtatat tcagagcact ctttacaggg
     3241 gtgggtggcg gttctacact tacacagcag ctcgtaaaga atacgcttct aagtgatcag
     3301 catagttacg ttcgtaagta tcaggagctg tttatgtcga ttgctgttga gcaacactac
     3361 agcaaagatc aaatcttaga tatgtatctg aattctgtat tctttggtga gaatgcgttt
     3421 gggatcgagg acgcggcaaa gctctacttc ggtaaagtac cgaaggatct tgatcttgct
     3481 gaaagtgcaa tgctcgtagg tgtacttccg gcaccaaatg cttattcgcc gattagtggc
     3541 aatgcaacct atgctaaaga acgccaaaca acggtactcg cacgcatggt aacaaatggc
     3601 tatatcaccg aagagcagaa aacagcagcc ctcgctgaaa cgctagtata tgcttcaaca
     3661 gcgcagtcaa tcaactcagt tgcaccgcac tttgcccaaa tggttatcga tgagttaagt
     3721 aagaagtatg gctatgaaac tgtaatgcgt tctggctacc aggtgaaaac tacgcttgat
     3781 ttaacggcac agcaaaatct tctcgatagc gtgggcgcgg gtatggcgca catcaacgcg
     3841 atgggtggta gcaatgcaag cggcatcgtg attgatccag ctagcggtgg gatacgcgca
     3901 cttgtgggta gcgccaacta tgatgatcca agctggggca aggtgaatat ggtaacaacg
     3961 gcgcgtcagc caggttcgag ctttaagcca atttactatt caaacgcgct tgcggatggt
     4021 acgattacgc cagcgacagt attgcaagat aaggtgattg attttggtgg cggctatgtg
     4081 ccgcgtgatg ctgaccataa tgaagcaagt cggggccagg taacaacgcg tcaggcgctt
     4141 gattggtcgc tgaacatccc aagtatccat gtgatgcagt cgtatggcgt atcgaagaca
     4201 atacaagcag cgaaaaatct cggtattacg actattgacg caagcaaaaa ctacgggctc
     4261 acgctagctc ttggttcggc agaagtacct cttgagcaaa tggcccacgc ctatgctgca
     4321 tttgctaacc aaggtcaaca atatgacgta tcaacaatta atcaggtcga agataagtat
     4381 ggcaagaaca tctttaagag cctaccgata agcaagcagg taatcagtaa agaaggcgca
     4441 tatttgcttt ctagcatcct aagcgacaac aaaacacgtt cacatatctt tggctcgagt
     4501 cttacggtat ccggtcacac tgttgctgta aaaactggta caacaaatga tgatagggat
     4561 gcatggacaa tcgggtataa cccacagtat gtagtaggcg tatgggttgg taacaatgat
     4621 aatacagtaa tggcaagcgg cggtagtgat atggctggac caatttggcg cggcacaatg
     4681 acaaaactgc tctcgggcaa aaaagacgtt ccctttgtag taccaagtgg tattacccag
     4741 cggcaagtat gtagcgggag cggtggactt gcagacaaag ctggtcctaa cacctattct
     4801 gagtacttta tgccaggtgc gctgccaaca aatagttgcc aggcagcacc aattatgatg
     4861 agcgtgtgcg acttgcagac tgggaaagta acatcaatcg acgaatcagc attcgatagc
     4921 acgaagcagt caaaggatac ggcgaactgc aaggcgccta ccgagcaagc ctgcaacctc
     4981 tctacgggca aggttgaaac gatcaacaca tcagactttg acggcactaa atactcgacc
     5041 gatacggcaa actgtaagac gagcagtaat aatagtgatg gaactcatag tgacacggta
     5101 acagcgtgtg acatgcaaac gggccaatgg gtacagatac cgcagagcca ggttgacggt
     5161 acgcgctata tgacggggat ggccacctgt caggtgagcc actaatctgt tacaatacct
     5221 actatgattg cacatattgc tggggtagtg gctgaaaaat ttggagggtc agttattgtt
     5281 gatgtagttg gtgttgggta tgagattaac gtcacggcac ttgactatga ggcggtgacc
     5341 cttgatcagc aagtaaaatt ctacacatat catcacattc gcgaacaaag ccaagagcta
     5401 ttcggttttt caagtctagc agcaaaaaag ctcttcgaaa tgttgattac agtgcagggc
     5461 gtagggccaa aagcagcgct tgcgattttg agtctgggtg aagcagagca tgtgcgaaat
     5521 gctattgcga atgctgatag catgtatctt gcaaaggcct ccggtgtcgg caagaaaaca
     5581 gccgagcgtg tggtagtaga tcttagtgat aaagtcggca tgccggtcgt gtataaacag
     5641 gaacaagcag cacagaccga actcaataca agcgacgaag cactcgaagc attgattgcc
     5701 ctaggctaca accttgcaga tgcaacaaaa gcactcgatg gcatcgacac aggccttcct
     5761 actgctaagc gggttacgga agcgcttcgg ggctagtatc tgctacaata taagcaatgg
     5821 ctattgagag aattgtagat accagcaccc acgatgagga tagcgacgag cagattattg
     5881 aaatcactct gcgaccccag aattttgatg actacattgg ccaggagcgt ttgaagaaaa
     5941 atcttcgtct cgcaatcgat gcagcaaaga aacgtggcga gccaatcgac cacgttctgc
     6001 tatacgggcc tcctggactc ggtaaaacaa ccatggcaag cgtgattgct aacgagatgg
     6061 gggcagggct tcgtattacg agtggtcccg cgattgaacg tgcgggtgat cttgcaagca
     6121 tcctgacgaa cctccaagac ggtgacattc tatttattga cgaaatccac cgcttaagtc
     6181 gtgtagttga agaggttcta tattcggcga tggaagactt caaacttgat attgttattg
     6241 gtaaagggcc ggcggcacga agtatccgtt tggacctgcc gaagtttacg gtgattggtg
     6301 caacgacgcg cacgggtagc ctggcggcac cactgcgcga tcgattcggg catatttatc
     6361 gcttggagtt ctatactcct gatgaaattg gtaaaattat cacgcgttcg gcaaagattt
     6421 tggagtcgag aatcgatgct gagtctgcgg cgctactctc aacgcgcgca cggctaacgc
     6481 cacgtattgc gaaccgtttg ttcaagcgag ttcgtgattt cgctgatgtg aatggtgacg
     6541 gtattatcga tacaaccatg actcatggcg cgctgcaaat gatggagatt gatgatctcg
     6601 ggctcgaccc agcagaccga aacttattag agagtataat agagcattat ggcgataggc
     6661 cagttggcct caatacaatc gctgcactaa ctggtgatga gatgtctaca attgaggatt
     6721 tctacgagcc gtacttaata cagatcgggt ttatcgagcg gacgccgcgc ggacgccaag
     6781 taacacaaaa ggcaaaacgg cacttacaaa agcaataata agagggggat tatctatgaa
     6841 aaaagacgac cagaccaacg acaagcctgt cgcgtatgac acagatggta agccattatt
     6901 tgcaacgccg caacagccaa cagaaccaga ggttcagcga cagatagtac acattgcgag
     6961 gtcaaccgac ccaattaggc aagaggtcag ccctgagata cagaagaaac acaacgagtc
     7021 gatgcggtta tatccaaacc ttaatctcag cgacgctgag tatgtgatta gttccgtgcg
     7081 tcggcatcca attggcctat tcgcgccagt gtttgtcaca ggtctacttg tagcgatgat
     7141 tgcaagtttc ttagcaaact actataacat tatgcaatta ctgcacattg tgaatatacc
     7201 gaagttcggc acggttctcc tgctcggtgt ccttgctatc atacttgtgc taatgggggg
     7261 ctatattgca atctgggtat accttagtaa ccagttcttc cttacaaacg aaagtgtaat
     7321 tcaggagatc cagcttacat tattctctcg tcgcgagcaa acggtgagcc tccttaacat
     7381 cgaggatgca agctttcttc aaacaggaat tttgcagacg atgtttaatt atggctcgat
     7441 tcgcttgagt accgagggcg aggaaacaac ctatcgcttt tcatatgttg ccaatccgcg
     7501 cgctgaaatt gcgcagctta ataatgcagt tgaggcattt aaaaacggtc gaccagttga
     7561 agtggttgaa gcaaacgaga aaaactagtt attagtgtga ctgcgatttg tttttacgta
     7621 gtctgattcg cgttccataa tactacggag cgtataacct gtgcagtggc catcggtaga
     7681 acagcctgta gcatcgtagc gatagtctga atcggactcc ccg
//
LOCUS       DATIIX010000098         3085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_469041, whole genome shotgun sequence.
ACCESSION   DATIIX010000098 DATIIX010000000
VERSION     DATIIX010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469041"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VLF64_02760"
     CDS             complement(<1..834)
                     /locus_tag="VLF64_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSW90893.1"
                     /translation="MSDVREVVMIGAGPSALSAAIYTTREDIDTVLYEKGVVGGLAAI
                     TDKVDNYPGFPDGIEGMTLAEQLQKQAERFGANIELGDVSALRRDGDEIIVTVDGNEV
                     RAKTVLIATGSDYSKLGIPGEAEYYGRGVHYCATCDGAFYRDKRLVVVGGGNSGVQET
                     IFLTRFASHIDLLVRSTIKASQVLQDDLQQYIESGKVTVHLGTTPDEIVTQDGKVTAV
                     RVTKDGEQSNVDTDGVFVFIGLKPNTQFLKGSDVELDELGLVKTNEKLETNVQGVYAS
                     GD"
     gene            complement(850..1542)
                     /locus_tag="VLF64_02765"
     CDS             complement(850..1542)
                     /locus_tag="VLF64_02765"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HSW90894.1"
                     /translation="MATTKAQRYGILTILIVTVVGTVGSFAVMILTSKSSAENQAKYQ
                     TALNKYTEVQKGYQAKVQAQADELSGKYYDTVNAYESRPAKFDINSVKDVGTEDLVAG
                     DGEEITGATAFAAYYIGWDANGRIFDQSVDTSAKKLKSPLPIDGGLDKASLIEGWKTG
                     MKGMRIGGIRLVTIPSDKAYGASGQTDSNGQQTIAPNMPLKFIVMAIPQPAAISQPDT
                     TKLLQEMQEARQ"
     gene            complement(1631..2827)
                     /locus_tag="VLF64_02770"
     CDS             complement(1631..2827)
                     /locus_tag="VLF64_02770"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSW90895.1"
                     /translation="MQITIVGGGFGGIRAALKLAREKSNEITLISDRPEFQYYPTLYS
                     SATGHSHLESWVPLGEIFAEHENVKVHIDTIETLQVSDKTLRGKSGATYKYDTLILAM
                     GVVTTYFGIPGLETYAYGIKSEAEIKHLKQRLYTDIAENHELDRNYVVIGAGPTGVEL
                     SAALGTYIQRLSKKYRVKGRGPRVRLIEASPRVLPRMSELSSRVVEHRLRSLGVDVET
                     GKKVEGENATELVVSGKPIASHTVIWTSGVTNNPFFAAHPNVFKLAKNGRVEVDAYLK
                     AYNSIYVIGDNAATPYTGLAQTAIHDGDFVAKNLIRKSHGKKVKKYHTSKPIPVVPVG
                     ANWAAVEWRGIKVYGFIGGLIRRAADAIGYNDLLPLGRTIGAWTASMVYEEDYFTPTV
                     KDKKLR"
BASE COUNT          745 a          821 c          645 g          874 t
ORIGIN      
        1 gtcaccacta gcatacacac cctgaacatt cgtctcgagc ttctcattcg ttttaacaag
       61 tccaagctca tcaagctcaa catcgctgcc tttaaggaac tgcgtattcg gtttgagccc
      121 gataaataca aacacaccgt ctgtgtcgac attggactgc tccccgtctt ttgtgacgcg
      181 cactgctgta actttaccgt cttgcgtcac gatttcatcc ggagttgtac ctagatgaac
      241 cgttacctta ccactctcga tgtactgctg taaatcatct tgtaacacct ggctggcttt
      301 gattgtgcta cgcactagta ggtcgatgtg gctcgcgaag cgcgtcaaga aaatcgtttc
      361 ctgtactccg gaattaccac caccgacaac aaccaatcgc ttatcacggt aaaacgcacc
      421 atcacaagtt gcacagtaat gaacgccgcg gccgtagtat tcggcttcgc caggaatacc
      481 aagcttgctg tagtcgctac cagtcgcgat aagtacggtt ttagcgcgaa cttcattacc
      541 atcaacagtt acaataattt catcgccatc acggcgcagt gcacttacat caccgagctc
      601 gatgttcgca ccgaagcgct cggcttgttt ttgcagctgc tcagcaagtg tcatgccctc
      661 aataccatcc gggaatccag ggtaattgtc gactttatcg gtaatagccg caaggccacc
      721 aacgacgccc ttttcataga gcaccgtatc gatatcctca cgcgttgtat agattgccgc
      781 gcttaatgcg cttggcccag caccaatcat taccacttct cgtacatcac tcattacccg
      841 tactccttat tactgtcgtg cttcttgcat ttcttgtaaa agcttagttg tgtctggctg
      901 tgagatcgcc gccggctgcg ggattgccat aacgataaac tttagtggca tgtttggtgc
      961 aattgtctgt tgaccatttg agtcagtctg accgcttgca ccgtaggctt tatctgatgg
     1021 aatcgtaacc aaacgaatac caccgatacg catacctttc atacccgttt tccaaccctc
     1081 aattaaactc gctttgtcga ggccaccgtc aataggtagc ggcgatttca acttcttcgc
     1141 agacgtatcg acactctggt caaaaatgcg accgttcgca tcccagccaa tatagtatgc
     1201 agcgaacgct gtagcaccag taatctcttc accgtctcca gccacgagat cttcggtgcc
     1261 gacatccttc acgctgttta tatcaaactt agcaggacgc gattcgtagg cgttaacggt
     1321 atcataatac ttgccggaca gttcgtcagc ttgcgcttgg actttggcct gatatccttt
     1381 ttgaacctcg gtgtatttat ttagagctgt ttggtacttc gcctgattct cagcgctact
     1441 tttgcttgtc aaaatcatta cagcaaacga accaacagtg cccacaacag tcacgattaa
     1501 aatcgtcaaa ataccatagc gctgcgcttt tgttgttgcc ataaactccc tcgttttgtt
     1561 ctagtctcgt ctctacagta taccatgagt cacactatat atagcggttt ttgtaaaatt
     1621 tacaccacta ctagcgtagt ttcttgtcct tgacagttgg cgtaaagtaa tcttcttcat
     1681 acaccatact tgcggtccac gcaccgatag tcctgccaag tggtaggaga tcattatacc
     1741 caatcgcatc agccgcacga cggattaaac cgccgataaa tccataaacc ttgatacctc
     1801 gccattctac agccgcccag tttgccccta ccggaacaac cgggattggt ttagatgtat
     1861 gatatttctt caccttcttg ccgtggcttt tgcgaataag gttctttgcc acaaaatcac
     1921 cgtcgtgaat tgccgtttgt gctagcccag tgtacggcgt cgctgcgtta tccccaatga
     1981 cgtagatgct attgtaggcc ttcaagtagg cgtcaacttc aacgcgtccg tttttggcga
     2041 gtttgaagac attgggatgc gctgcaaaga atggattatt tgtcacgccc gaggtccaga
     2101 tcactgtgtg actagctatc ggcttgccgc tcacaacaag ctctgttgcg ttctcgcctt
     2161 caaccttctt gcccgtttct acatcaacgc caaggctacg caagcgatgc tcgacaactc
     2221 gactcgacaa ttcactcatt cgaggcaaca ctcgtggtga ggcctcaata agccgcactc
     2281 ttggcccgcg accttttact ctatattttt tgctaagacg ttggatatag gtacctaacg
     2341 ctgcagagag ttcgacgcca gtcggcccag caccgatcac tacatagtta cgatcgagct
     2401 cgtgattttc agcaatgtct gtatataggc gctgctttaa gtgtttgatc tcggcttcac
     2461 ttttaatacc gtatgcatat gtttccagac cagggatgcc gaagtacgtc gttaccaccc
     2521 ccattgctaa aataagcgta tcgtatttat atgtggcgcc cgacttaccg cgcaatgtct
     2581 tgtctgaaac ctgaagtgtt tcgatagtat caatatgaac ttttacattt tcatgctctg
     2641 cgaaaatttc accgagcgga acccatgatt ctaaatggct atgaccggtt gcgctactgt
     2701 aaagtgtagg gtaatactga aattctggac gatcagaaat aagtgtaatc tcgttggatt
     2761 tctcacgtgc taatttaagc gctgctcgaa taccgccaaa cccgccaccg acaatcgtaa
     2821 tctgcatgtt tactgttcct tttttgagta gttatacgtt ttattgtagt atagcgctta
     2881 tgctactgca acagccacta cgctggaggc aagcattacc attggcatat agagtgacgt
     2941 accaaacgtc cttcgtgccc atgcgtcggt accacgttgc tttgtgagaa gtattataca
     3001 tacccatgtg accgctgcgg ctgcaaacat tccggtggca actttcggta gtggagaaaa
     3061 tactaccaga gcgatcgttg cgagc
//
LOCUS       DATIIX010000099         2676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_275134, whole genome shotgun sequence.
ACCESSION   DATIIX010000099 DATIIX010000000
VERSION     DATIIX010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275134"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..632)
                     /locus_tag="VLF64_02775"
     CDS             complement(48..632)
                     /locus_tag="VLF64_02775"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HSW90896.1"
                     /translation="MSDLLDEIYQAIPNDPMNAEMSAKGYVPVYTASAKSRIVIVGQA
                     PGRKAQESMKPWNDASGVLLRSWLGVTDEQFYNPDLFALVPMDFYYPGKGAHGDLPPR
                     KGFAEKWHPKIFEHMPNVELIILVGAYSQKYYLGKGVGKNLTETVFAYHQYAPKFFPL
                     VHPSPLNIGWRKNNAWFETDIVPKLRTRVVEILK"
     gene            complement(648..983)
                     /gene="trxA"
                     /locus_tag="VLF64_02780"
     CDS             complement(648..983)
                     /gene="trxA"
                     /locus_tag="VLF64_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HSW90897.1"
                     /translation="MALYNTRTKQEFEDKVLNSKKTVLVDFWADWCPPCRAMAPILHD
                     LGNELDETIDIVKVDIEDGAENSALAVEYGISSIPNMHIFKGGKVVDHVIGMVPKVHL
                     VEVLNQAAK"
     gene            complement(1026..1610)
                     /locus_tag="VLF64_02785"
     CDS             complement(1026..1610)
                     /locus_tag="VLF64_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HSW90898.1"
                     /translation="MPHIHNEPDQHDMTVSGYIVRTDGNEPLCLVHMHKKIGKLMQIG
                     GHIELNETPWAAIAHELEEESGYQLNELKVVQYTADRVVDASNVSHPTPYLMNTHNVG
                     NEHYHSDLCFGFVARSLPVRNMADGESDDIRWLTLSELREAAKNGDALQDVSNMYTFL
                     IEHLNIYVLVPALDFSVDKPTIAAATYKRGAPGK"
     gene            complement(1610..2521)
                     /locus_tag="VLF64_02790"
     CDS             complement(1610..2521)
                     /locus_tag="VLF64_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HSW90899.1"
                     /translation="MQKILLYYKFTPIADPEAVKLWQKTLCESLNLRGRILVSAQGLN
                     GTVGGDIDGLKAYIKATKQYPGFKGIAFKWSEGSREDFPRLSVKSRRELVGFKNSDDE
                     FAVDENGVIDGGQHIKPEQVHELVEKYGNDVIFFDGRNEHEAKIGKFKNAIVPNTNTS
                     RDFISELESDKYDDIKDKKIITYCTGGIRCEVISAMMKRRGFSDVYQIDGGIVKYGET
                     YGDDGLWEGSLRVFDNRMTVNFSDHAKTIGECSHCSAKTANFENCALPECNELVLICE
                     TCKEQPDLLFHTTLCRERQTDKIGAMA"
BASE COUNT          641 a          637 c          604 g          794 t
ORIGIN      
        1 gtcaggtgtt ccgggcacca ctcccctcct cactgagggg attttgctta tttgagaatc
       61 tcgacaacgc gtgtacggag ttttggtacg atatctgttt cgaaccatgc gttgtttttt
      121 cgccacccga tattcagcgg actcggatga acaagtggaa aaaactttgg cgcatactga
      181 tggtacgcaa agactgtttc agtgagattc ttaccgacgc ctttgccaag atagtatttt
      241 tgtgaatatg caccgaccag gataattagc tcaacatttg gcatgtgctc aaatattttt
      301 gggtgccatt tttcggcgaa acctttacgg ggtgggagat cgccgtgcgc gcctttaccc
      361 gggtagtaaa aatccattgg cacaagggca aataaatctg ggttgtagaa ttgctcgtct
      421 gttacgccaa gccacgaacg aagtagcacg ccgctcgcat cgttccatgg cttcatggat
      481 tcctgtgctt ttcgccctgg ggcctggccg acgataacaa tccgcgactt tgcggaggca
      541 gtatacactg gcacatatcc cttggcgctc atttcggcgt tcattgggtc attaggaatc
      601 gcttggtaga tttcgtcaag caggtcgctc attattgcct aaaagaatta cttagcagcc
      661 tggttgagca cttcaacaag gtgtactttt ggaaccatac cgattacatg gtcgacaact
      721 ttgccgccct taaaaatatg catgtttgga atgctgctga taccatattc gacagcaagc
      781 gcagaattct cggcgccatc ttcgatatca accttgacaa tgtcgattgt ctcgtcgagt
      841 tcgttgccta aatcatgaag aatcggcgcc atcgcacggc aaggcggaca ccaatctgcc
      901 caaaaatcaa caaggactgt ttttttgctg ttcagtacct tatcttcaaa ctcttgcttg
      961 gttcgtgtgt tatataacgc cataatactt taagtatatc accattttaa tgtggcgcga
     1021 acgcattact taccaggtgc accgcgctta tatgtggcgg ctgcaatagt tggcttatca
     1081 actgaaaagt ctagtgccgg cacgagtacg tatatattga ggtgctcgat gagaaatgtg
     1141 tacatattgc ttacatcttg gagcgcatca ccgtttttag cggcctcgcg aagttcgctg
     1201 agtgtgagcc agcgtatatc atcagattcg ccatcagcca tattccgaac aggcaagctt
     1261 cgagctacaa agccaaaaca taaatctgag tggtaatgtt cgttacctac attgtgcgta
     1321 ttcattaagt acggtgtcgg gtgactaaca ttacttgcat cgacgacgcg gtcggctgtg
     1381 tattgcacaa ccttcagttc gtttagctga tacccacttt cttcttcgag ttcatgcgca
     1441 attgctgccc atggtgtctc gttgagttca atgtggccac cgatctgcat tagtttgcca
     1501 atctttttat gcatatgtac gaggcaaagc ggctcgttgc catcagtacg cacaatatac
     1561 ccactcactg tcatatcatg ctggtcgggt tcgttatgaa tatgtggcat tatgccatag
     1621 ctccaatctt gtcagtttgt ctttcgcggc aaagggtcgt gtggaatagc aaatctggtt
     1681 gctctttgca ggtttcgcag attagtacaa gctcgttaca ttctggcaat gcacagtttt
     1741 caaagttggc ggttttcgcg ctacagtggc tgcattcgcc gattgttttg gcgtgatcgc
     1801 tgaaattcac tgtcatacga ttatcgaata cacggagtga accttcccac aggccatcgt
     1861 cgccgtaggt ctcaccgtat ttcacaatac cgccgtcgat ctggtagaca tcactgaaac
     1921 cgcgcctttt catcattgcg ctaatcactt cacagcgtat accgcctgtg caatatgtga
     1981 tgattttttt gtctttaatg tcgtcatact tgtcactctc tagttcagag ataaaatcgc
     2041 gtgaggtgtt agtatttggc acgatagcgt ttttgaactt gccaattttt gcctcatgct
     2101 cattgcgccc atcaaagaat atgacatcgt taccgtattt ctcaacaagc tcgtgtactt
     2161 gctctggctt aatatgttgg ccgccgtcaa tcacaccgtt ttcatcgaca gcaaactcat
     2221 catcgctatt cttaaaccca acgagctcac gacgagattt tacgctgagt cgcgggaagt
     2281 cttcacggct gccctcgctc cacttaaatg caatgccttt gaatcccgga tattgtttcg
     2341 tcgctttaat gtatgccttt aagccatcga tgtcgccgcc aactgttcca ttgagaccct
     2401 gggcgctcac taaaattcgg ccacgtaaat taagtgattc acagagtgtt ttctgccata
     2461 atttcactgc ttctgggtcg gcaataggag tgaatttgta gtagagaaga attttttgca
     2521 ttggcattta ttataccact tttgcctaaa ataatctagc ggacgtgcga gactgcttgc
     2581 tggcgatgca ctgcgcgaag cgataacgac attgccgtaa ctgttaatga agtaaacagt
     2641 agtccgctca gacaaaggtg taatacattt gcgcct
//
LOCUS       DATIIX010000100         6084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_357655, whole genome shotgun sequence.
ACCESSION   DATIIX010000100 DATIIX010000000
VERSION     DATIIX010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357655"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="VLF64_02795"
     CDS             <1..1360
                     /locus_tag="VLF64_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HSW90900.1"
                     /translation="FYYDGSKNWWNRGKTGPDTTPIGDPCGPDSEMFYDFGTPHNPEF
                     GEFCHPNCDCGRFMEIGNNVFMAYKKVGDGRFEELENKNVDHGSGLERIAAAKLNSPD
                     VFKISLMLPIVQKLEKLSGKNYDSHTESMRVIADHLRAATFLAVDGCTPSNKEQGYVM
                     RRLLRRAIRFSFDLGIEQNFLQEIVPVIADLYVNDFPEVKEHRDAVIAVLVKEEKAFR
                     QTLRKGLKELEKFKNQTIAGEQLFTLYDTYGFPVELSTEEAFKQSITVSPSWREEFDT
                     KMEEQRNRSRTATKGEFKGGLGGQDLIHKKYHTATHLLQSALREIFGSELRQHGSNIT
                     EERLRFDFNIDRKMTPEEVARAEELVNGWIAEDLPVSFKQYPTQVALDMGAIGPFGER
                     YGEEVKVYQMGEGNHVASLEICGGPHVDHTGQLAEDGKKFKITKEEASSAGIRRIKAV
                     LV"
     gene            1700..2962
                     /locus_tag="VLF64_02800"
     CDS             1700..2962
                     /locus_tag="VLF64_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HSW90901.1"
                     /translation="MVFDKILNKSKESQLDYLVGLDIGTEYVKALIAQVSGDQLEIIG
                     VGKSHQELGDMHAGAIADIAGVVRNCEEALAHAEEAAGLQAKRCVIGIAGELVKGVTN
                     TIRYRRPQPDRPLDENEMEFIIEKVQDRAATKAQKQIALETGNDDVEVKLVNSALVSI
                     HIDGYKVSNPIGFQGKDVAVQIYTAFAPMVHIGALEKVADELDLELVAVAAEPFAVSR
                     SVLGTDVDSAFTAILADVGGGTTDIAVVNDGGVEGTKMFGIGGRSFTNTIATELEIPY
                     EQAEKLKVNVGDDKVKASVKKDVAKAVDKTLDVWLAGVELALSEFDSVDHLPSRILLC
                     GGGASLEQLMTALEQREWYKDLPFTKKPSVHHIKPTDVVGMSDITGEANDHTFITAMG
                     LLRVGYDTIIGISETGSLRDKLNRMLRI"
     gene            2973..4610
                     /locus_tag="VLF64_02805"
     CDS             2973..4610
                     /locus_tag="VLF64_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90902.1"
                     /translation="MQKDVIYIDTEDDITAIIGKVRASGRKIVALVPPKRIGAIQSAV
                     NLKLVQRAADQAGKHLVLISNNAALTALAGSAKIPVAKNLQSKPEIAEIPAIDIDDGE
                     DVIDGSDVLATPSVPGKPNLDDATAATGDDLGQGMDAKAPARPIRATPAVPRTTGGRG
                     IKIPNFDKFRKKLFLIVAAVVLLIGFLIWAIVFAPAAKITITARTSDSSLNTLIKMGD
                     SITTSLRDGTVKSLTKTAKKDVSVSFAATGTKNVGDKASGSVVMQACVAGLSTPSSVP
                     AGTTLTANSNEYTTGSAASFAYDHVGNGCIVYKSGSVPITASASGVAYNTSGSFTVAG
                     GSVTAQGSASGGTDKTVQVVQQSDVDTVSNNILSSSDNDTAKKSLQDAFGSDYVVIDS
                     SFKSDTSAVKPSPAVGTEADSGKGALVGSVTFSMAAIPKKELGTFLDAYFAQQIDGQD
                     NQKVYDNGLKSVSFTNVNAVESGFTANITTNGKIGPKIDDKALQEYAKGRRIGEIQSY
                     VQNINGVENVDIKFSPFWVNSAPNNVNKIHVEFKVNG"
     gene            4603..5004
                     /gene="ruvX"
                     /locus_tag="VLF64_02810"
     CDS             4603..5004
                     /gene="ruvX"
                     /locus_tag="VLF64_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HSW90903.1"
                     /translation="MAKQNFLALDVGERRIGVAIADSSIKIAIAYETIIVDGNELKRI
                     AEIISYENIDIVVIGYPRNQSGEPTKQTEYVEAFAELLVDIAPEIVFQDESLTSVKAE
                     ETLKRQGKPFEKGDIDALAASFILEDYLEQH"
     gene            5004..>6084
                     /locus_tag="VLF64_02815"
     CDS             5004..>6084
                     /locus_tag="VLF64_02815"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HSW90904.1"
                     /translation="MDGFKRPSQQAPRPLRPVAPQSSPNLQSQQTPMQPQPVVLQPQA
                     VSGATNPSVAQPLAKSTENKSLSLAKKHRWMWWIAGIIIFSIVVAAIGYLWYVNALTP
                     VNAKDRTVQQVQINEGSSFGFVSGRLEERKLIRSSLALQIYAYLHHEAGNLKQGSCNL
                     TPSESSREILKKLTGGCHDFKLVTFYPGATIEKSKNLKEGEKDFSVRGSLEAAGFSDL
                     DITAALDATYDSPLFADKPTSASLEGYIYGDTYYINADSGPAEALKDSFNEMEKVVEK
                     NNLVTDYRAHGLTLYQGITLASIIQRELSCDNLDETKQTICYENQQKVAQVFYSRLAQ
                     GITLGSDVTFIYAAGLTGVAPTPSLD"
BASE COUNT         1731 a         1391 c         1501 g         1461 t
ORIGIN      
        1 tttctattac gacggaagca agaactggtg gaatcgtggt aaaactggcc ccgacacaac
       61 gccgattggt gacccatgtg gcccagatag cgaaatgttc tacgactttg gtacgcctca
      121 taatcctgaa ttcggtgaat tttgtcaccc aaactgtgac tgtggtcgct ttatggaaat
      181 tggcaacaat gtctttatgg catacaagaa agttggcgat ggtcggtttg aagagcttga
      241 gaataaaaac gttgaccatg gttccggcct ggaacgtatt gctgcagcca aacttaacag
      301 tcctgatgta ttcaaaatta gcctaatgct ccccatcgtc caaaagctcg aaaaacttag
      361 cgggaaaaac tacgatagcc acaccgaaag tatgcgggtt atcgccgatc accttcgtgc
      421 ggcaacattc cttgcagttg acggttgtac gccaagcaac aaagaacaag gctatgtcat
      481 gcgccgactg ttgcgccgtg cgattcgctt tagtttcgat cttggtatcg agcaaaactt
      541 cttacaagag attgtcccag taattgccga tctctacgtc aatgacttcc cagaggtaaa
      601 agaacatcgt gatgcggtta tcgctgtact cgtgaaagaa gaaaaagcat ttcgtcaaac
      661 gcttcgcaag ggtcttaaag aactcgaaaa gtttaagaac cagacaattg ccggtgagca
      721 gctatttaca ctctacgaca cctacggttt cccagtagag ctttcaactg aagaagcctt
      781 caagcaaagt atcacagtat ctccctcgtg gcgcgaggag ttcgacacta aaatggaaga
      841 acaacgtaac cgtagtcgca ctgcaaccaa gggtgagttc aagggtggtc ttggcgggca
      901 agacttaatc cacaaaaagt atcacactgc gacacatctt ttacagtctg cactccgtga
      961 aatcttcggt tcagagcttc gtcagcacgg cagcaacatc accgaagaac gcctccgctt
     1021 cgatttcaat atcgatcgca aaatgacccc tgaagaagtt gctcgtgccg aagaactcgt
     1081 gaacggctgg atcgccgaag accttcctgt gtcatttaag cagtatccaa cccaggtggc
     1141 acttgatatg ggcgccatcg ggccatttgg tgaacgctat ggcgaagaag taaaggtata
     1201 tcagatgggc gaaggcaatc acgttgcttc gctcgaaatc tgtggcggcc cgcacgttga
     1261 tcataccggt caactcgctg aggacggcaa gaaatttaag atcactaagg aagaagcttc
     1321 aagcgcgggt attcgccgca ttaaagccgt acttgtatag actagaggta ttccacgaga
     1381 tattctcgct attctgacga gtctaccttc tactatgtag cgccgaaatt ttcggtttct
     1441 aggaggcaaa ataataaagt taaaatcttt ctctatagaa ttcttgggaa gcatgacatg
     1501 cttgacccgt actaatatgt gccatagaat ccaaggtcgg ccatggtgat acgctcctat
     1561 ggccagccag cccccaccca agagtcgggc ggggctctac aactcagttg caaatgtaat
     1621 tgtatatatt cttgtctcgt ggtgaaacgg ttagattatc gttttgcatt tttaaaaagt
     1681 gctataataa acaatgatta tggtttttga taaaattctt aataaatcaa aagaatcaca
     1741 gttagactat cttgtcgggc ttgatattgg tactgagtat gtgaaggcgc taattgctca
     1801 agtctctggt gatcagcttg aaattattgg tgttggtaag tcacaccagg aacttggtga
     1861 catgcacgcc ggtgcgattg ctgacatcgc cggtgttgta cgtaactgcg aagaagcact
     1921 agctcatgcc gaagaagcgg ccggcttgca ggcaaagcgc tgcgtaattg gtattgctgg
     1981 cgaactggta aaaggcgtaa cgaatacaat ccgctaccgc cgaccgcagc cagaccgtcc
     2041 gctcgacgaa aacgagatgg aattcattat cgaaaaagtc caagatcgtg ccgctacaaa
     2101 agcgcagaag cagatcgcgc ttgaaacggg caacgatgac gtcgaagtaa agcttgtgaa
     2161 ttctgcgctt gtcagcattc acattgatgg atacaaagtg agcaacccta tcggcttcca
     2221 aggcaaagac gtagcagtac agatttacac cgcctttgca ccgatggtac atatcggcgc
     2281 actcgaaaag gttgctgacg agcttgacct cgagcttgtg gcggttgctg cggagccatt
     2341 tgcggtaagc cgtagtgtac tcggtaccga tgttgatagc gcctttacgg caatcttagc
     2401 tgacgtcggt ggcggtacta ccgatattgc cgtcgtgaat gatggtggcg ttgaaggtac
     2461 gaaaatgttt ggtattggcg gacgcagctt tacaaatact attgcgaccg agcttgaaat
     2521 tccttacgaa caggccgaga agctcaaggt aaatgtaggc gacgacaaag tgaaagcttc
     2581 ggttaaaaaa gacgttgcga aggcggtcga taaaacactc gatgtttggc ttgcgggcgt
     2641 tgagctagcg ctgagcgaat ttgattctgt tgatcacctc ccgtctcgaa tattactttg
     2701 tggtggcggt gcgagtcttg agcaacttat gacggcgctt gaacagcgag aatggtataa
     2761 agaccttccg ttcactaaga agccctcggt gcatcatatt aagccaactg atgtcgtcgg
     2821 catgtcagat attaccggtg aagcaaatga ccataccttt attacagcga tgggattgct
     2881 acgcgtcggg tatgatacaa taattgggat aagcgaaacg ggttcattgc gcgataagct
     2941 caatcgaatg cttcgtattt aactgaggta gcatgcaaaa agacgttatc tatatcgata
     3001 ccgaagacga catcaccgcc atcatcggca aggtgcgcgc aagtggtcgc aaaattgttg
     3061 cactcgttcc accaaaacgg attggcgcta ttcagagcgc ggtaaaccta aagctcgtac
     3121 agcgcgcagc ggatcaagca ggcaagcatc tcgtacttat ttcgaataac gcggcgctta
     3181 cggcgcttgc gggtagcgca aagatccctg tcgcgaaaaa ccttcagagt aaacctgaaa
     3241 ttgctgaaat cccagcgatt gatattgatg atggtgaaga tgtaattgac ggtagtgatg
     3301 tactcgctac accaagtgtg cccggcaaac caaacctcga tgatgcaact gctgcaacgg
     3361 gtgatgattt agggcagggt atggatgcaa aggcgccagc gcgacctatc agagccactc
     3421 cggccgtccc gcgaactaca ggtggacgcg gaataaaaat cccgaacttc gataaatttc
     3481 gcaagaaact atttttaatt gtcgctgcag tagtattgct tataggcttc ttaatctggg
     3541 cgattgtatt tgcacccgct gctaaaatta cgattaccgc gcgcacgtca gatagctcgc
     3601 tcaatacact tatcaaaatg ggcgacagca tcacaacaag cttgcgcgat ggtacagtaa
     3661 aatccttaac gaagacagcc aagaaagatg tatcagtgtc atttgctgca acaggcacta
     3721 aaaacgtagg cgacaaagct tcgggtagtg ttgtaatgca ggcctgtgta gctggactga
     3781 gtacgccatc aagcgtacct gcgggcacta cgcttacggc aaacagcaat gaatacacaa
     3841 ctggatcagc ggcatcattt gcttatgatc atgtcggtaa tggctgtatt gtttataaat
     3901 ctggcagtgt tccgattact gcaagtgcaa gcggtgtggc atataacacg tctggtagtt
     3961 tcactgttgc tggcggtagt gtaacggcgc aaggatctgc aagcggtggt acagacaaaa
     4021 ccgttcaggt tgttcagcaa agcgacgttg acacggtttc gaacaatatt ctcagtagct
     4081 cagataacga caccgcaaag aagtctcttc aagacgcttt tggcagtgat tatgtcgtga
     4141 ttgacagtag ttttaagagt gacacgagtg cagtcaagcc aagcccagcg gttggcaccg
     4201 aagcggacag cggcaagggt gctctcgtgg ggagcgtaac attctcaatg gcggcgatac
     4261 ctaagaaaga actcggtaca ttcctcgatg catacttcgc acagcagatt gacggtcaag
     4321 acaaccagaa ggtatacgac aatggcctaa agagtgtatc atttaccaat gtaaacgcgg
     4381 tggagagcgg ttttaccgcg aacattacca ctaacggtaa gattggcccg aaaattgatg
     4441 ataaggcgct tcaggaatac gcaaagggta ggcgaatcgg cgaaatccag agttacgtcc
     4501 aaaatattaa tggcgtcgaa aacgttgata tcaagttctc tccattctgg gtaaactctg
     4561 cacccaataa cgtgaacaaa atccacgttg agtttaaggt gaatggctaa acaaaacttc
     4621 cttgcgcttg atgttggcga gcggcgcata ggggttgcta ttgccgatag ctctattaaa
     4681 atcgctatcg cctacgaaac gattatcgtc gacggtaatg agctcaagcg tattgctgag
     4741 attatctcat atgaaaacat tgacattgtc gtgattggct atccgcgtaa ccaatccggc
     4801 gagccaacaa aacagaccga atatgttgaa gcgtttgctg agttactcgt tgacatcgca
     4861 ccagaaattg tgtttcaaga tgagtcactc acgagcgtaa aagcagaaga gaccctcaag
     4921 cgccagggga agccgttcga aaagggcgac attgatgccc ttgccgcaag ctttattcta
     4981 gaagattatc tggagcaaca ttgatggatg gcttcaagcg accatcacag caggcacctc
     5041 gtcccctgcg cccagttgca ccacaatctt cgccgaatct acaatctcaa cagactccga
     5101 tgcaaccgca gcctgttgta ttgcagccgc aagcggtatc tggcgcaacc aatccttcgg
     5161 tagcacagcc gcttgcaaaa tctacagaga ataaatcact ttcactagca aaaaaacacc
     5221 gttggatgtg gtggatagcg gggatcatca tattttcaat cgttgtggct gccatcggat
     5281 atctctggta tgtcaatgca ctcactcccg ttaatgcaaa ggatagaaca gttcagcagg
     5341 tgcaaatcaa tgagggcagc tcatttggct tcgtaagtgg tcgccttgaa gaacgcaaat
     5401 tgattcgcag ctcgctcgca ttacagatat acgcatatct ccatcatgaa gcgggcaatc
     5461 tcaagcaagg ctcttgtaac ctgacgccga gtgaatcatc tagggaaatc ctaaaaaaac
     5521 tcacaggggg atgccacgat ttcaaattag tgacatttta cccaggcgca acgattgaaa
     5581 aatcaaaaaa tctcaaagaa ggtgaaaaag atttctcagt tcggggaagt ctcgaagcgg
     5641 caggatttag cgacttggat atcacagcgg cccttgacgc cacgtatgat agccccctct
     5701 ttgctgacaa gcctacaagc gcttcgcttg aaggatatat atatggtgat acatattata
     5761 tcaatgccga ttctggccca gcagaggcgc taaaggattc atttaacgag atggagaagg
     5821 ttgtcgagaa gaataacctg gtcactgact atagggcgca cgggttgacc ctataccagg
     5881 gcatcacgct tgcatcaatc attcagcgcg aactgagctg tgataacctt gacgaaacca
     5941 aacaaaccat atgttacgaa aatcagcaaa aggtggcgca ggtattttat agccgtcttg
     6001 cccagggtat aaccctagga tcggatgtca catttatcta tgcagccgga cttacaggtg
     6061 ttgcaccaac gccaagcctc gatt
//
LOCUS       DATIIX010000101         6071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_246815, whole genome shotgun sequence.
ACCESSION   DATIIX010000101 DATIIX010000000
VERSION     DATIIX010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246815"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VLF64_02820"
     CDS             complement(<1..862)
                     /locus_tag="VLF64_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HSW90905.1"
                     /translation="MQPHASLRIFLPSIIISLLVGTFIGFQAPLDSLWIFLILVILEI
                     TFSFDNAVINSKILGRMSSFWQQLFLTVGIVFAVFIVRFVLPIVIVMISSGHMFGDVV
                     NMALNEPRHYAETLHDAAPIINAFGGSFLLMIGVNYFLDRNKDIHWLGHIERWLSRFG
                     TYDGFKILIMLLVALGLYATVDESLRSIVLASSTIGIVLHIALELFGEYFGGKQSNAS
                     HLAGAAAFASFLYLNVLDASFSLDGVIGAFAISTDVLLIMAGLGVGALWVRSLTIYLT
                     RANTLSHYRYL"
     gene            912..1835
                     /locus_tag="VLF64_02825"
     CDS             912..1835
                     /locus_tag="VLF64_02825"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HSW90906.1"
                     /translation="MITYYQRTSETASLTEQDKISSGSWVRSERPTTSDIETLHKFGI
                     DADLLTDALDPHEVPRIEHDDGWTYFITRVPDVGDNFNDYTTPMMFALGKDQTVTVSR
                     DSLGRLWLPFVEKTTISPGQQSKLFLSMVEIIFQSYQQRVTMINRQMRALTSNIQSLR
                     PHDISMIAEHERKLNDYLDALIPTNTALEKTLNPRFNLLRLSDDDQDMVEDLSVDIEQ
                     VIARCKSLLRTITNVRDSYRAVMDTRLNETMRVLTVVTLSLTIPTMISGVFGMNVALP
                     VDLHDGAIFWIVIVVSLIAAASVSAYFLRKR"
     gene            1836..2534
                     /locus_tag="VLF64_02830"
     CDS             1836..2534
                     /locus_tag="VLF64_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HSW90907.1"
                     /translation="MSLSLNVTNEYSYRARSRITVMTVITVCLVASLAVIAIKLARGV
                     QEHETLGMDKRILLTIHQHATPFLDWLAVHTTDIGSPLVAIAIALCIGFYLAYKREVI
                     RFFIIGGSMVGSAGLVSIIKSLIERPRPQLWHHLLSEKGFSFISGHATMSMTLAAAIV
                     AILWRTKWRWPAIIGGTVYASWVGFSRLYLGVHYPTDILGGWVVASAWVLVVTLTIHL
                     VFGDTISIPFAKKS"
     gene            2567..2815
                     /locus_tag="VLF64_02835"
     CDS             2567..2815
                     /locus_tag="VLF64_02835"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HSW90908.1"
                     /translation="MAFEHNVKLAGTVTVGPKGQVVIPAEVREQMQFGPGDKLVALYI
                     KDKKSVLLITEEQAQAFVTHMGEQFTQFKKDLEEGKES"
     gene            2815..4494
                     /locus_tag="VLF64_02840"
     CDS             2815..4494
                     /locus_tag="VLF64_02840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HSW90909.1"
                     /translation="MLHHFSLRQKIIVMVAVMSSLFLVALDQTVIATALSAIVREFKS
                     YSSLGFIVTAYMLTTTITVPLAGKLSDMYGRRPMLITGVSIFTIGSLLSGLSPSVEWL
                     IAWRALQGIGGGIITSQAFTIVGDLFTPRERGRWQGMIGAVFGMSSVIGPLIGGWLTD
                     KQHILGLVTDWRWTFFINVPVGIIAAIIIMANLPNIKHDAKHYPDYAGAAMMSIVLAS
                     VVLAVDNTKVIFKFLTDTGVELGLIQGILWSIVILGTGLFIAVERRAKQPILPLRFFK
                     KQVYTKLTAVTLLFGAAFMGSILYLTQFNQQVYGASASQAGYMLLPMVAGMMVSSIGT
                     GQIVSRLGRYKIFIVTGFTLATLSILALLTLEPSSPYWHEAVIMTFAGLGLGMAMPIL
                     SLAVQNEFEHQDLGAATSSVQLFRGLGSTIGTAIFSGMLTAGVLAAIGKPTDIPYIQT
                     LQRSPQSAQMLNGNIDADVLLQINTQKDTIADGANKAFDKIPAPAVRDETKKSFADQQ
                     NKYSAEVTDAFTKSLHQIFMLGAALMALSLVIVCFVKERPLRDSVNITPSE"
     gene            complement(4831..5589)
                     /locus_tag="VLF64_02845"
     CDS             complement(4831..5589)
                     /locus_tag="VLF64_02845"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HSW90910.1"
                     /translation="MTKRAWIIFAVICVALIGGLIYMSSMNKVDVAKVDALKIQSAMD
                     DNGKIADHTFGNTKSKVTLVEYGDYQCPGCGDAYPVIKQITEKYKDQLGFIFRNYPLS
                     TLHPNALAAATVAEEANLQGKFWEMHNKLYETQDSWNQLSGSDRTNYFVGLADAVGID
                     KSKLESALNGDSSDSNHISKKINFDMALGKKQGVSGTPTLYLNGKEVSSEYFKDDKIV
                     PQSVTGAQPVWSDATAFDTLVIKPVLQDSGMLSQ"
     gene            5676..>6071
                     /locus_tag="VLF64_02850"
     CDS             5676..>6071
                     /locus_tag="VLF64_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90911.1"
                     /translation="MQTFIVIPLCLTIVFLILAASISPRRSRLSRFELERRREEGDTS
                     AHGELERELAIADVVSFLRAVVALLLVFAVLLAVSTFGPFFGLIIGVILALGYGRMAQ
                     VDWVHGLADRMYQPYDESLVSFVQTQPWIG"
BASE COUNT         1617 a         1406 c         1404 g         1644 t
ORIGIN      
        1 caaggtagcg gtagtggcta agcgtatttg cacgcgtaag gtaaatggta agcgaccgaa
       61 cccacagagc gcccacgccg agaccagcca taatcagtag cacgtcggtg cttatagcaa
      121 atgcaccgat cactccatcg agtgagaatg atgcgtcaag tacattaagg tataagaatg
      181 atgcaaaggc tgctgctccg gctaggtgtg acgcatttga ttgtttgccg ccaaaatact
      241 caccaaagag ttcaagcgct atgtgtaata caatgccaat tgtagatgat gcaagtacga
      301 tagatcttag cgattcatca acagtcgcat ataggccaag cgccacaagc agcataatga
      361 gaatcttaaa gccgtcatag gtaccaaacc gagaaagcca tcgttcgata tggcccagcc
      421 agtgaatgtc tttgttgcgg tcgaggaaat agttgacccc aatcataagg aggaacgatc
      481 caccgaaggc attaataatt ggcgccgcgt cgtgcagcgt ctctgcgtag tggcgtggct
      541 cattgagcgc catattcacg acatctccaa acatgtggcc ggatgaaatc ataacaataa
      601 caattggtaa tacgaagcgc acaataaaca cggcaaagac gataccaacc gtcagaaaga
      661 gctgttgcca gaatgaggac atgcgaccga gaatcttact gttaataacc gcattgtcaa
      721 aactaaacgt gatctctaga attaccagaa tcagaaaaat ccaaagacta tcgagcggtg
      781 cctgaaaacc aatgaaggtt ccgaccagta acgagataat gatagatggg agaaaaatac
      841 ggagactagc gtgtggttgc atactttcaa gtatagcaaa gcttacactt acagtataaa
      901 ctatagggga gatgattacg tattaccaac gcaccagcga aacagcaagc ttgactgagc
      961 aagataagat ttcttctggt agttgggtac gatcagagcg tccgacgact agcgatatcg
     1021 aaacattgca caaatttggc atcgatgccg atctattgac tgacgccctt gacccgcacg
     1081 aagtacctcg tattgagcac gatgatggct ggacgtactt tattacccgt gtccctgatg
     1141 tcggcgataa ttttaatgat tacacgaccc caatgatgtt cgcgctcggt aaggatcaaa
     1201 cagtaaccgt gagccgtgat tcacttggtc gtttatggtt accatttgtt gaaaaaacga
     1261 cgatctcgcc agggcagcag tcgaaactct ttttaagtat ggtagaaatt atcttccagt
     1321 cgtaccaaca gcgggtgacg atgatcaatc gccaaatgcg cgcacttacc agcaatattc
     1381 aaagtctgcg cccacacgac atctcgatga ttgccgaaca cgagcgaaaa ctgaacgact
     1441 atcttgatgc actcatcccg acaaacactg cgctcgaaaa aacacttaac ccacgcttca
     1501 acctattgcg ccttagtgat gatgaccaag atatggttga ggatctttct gtggacattg
     1561 agcaggtaat tgcacgctgt aagtcgctcc ttcgtaccat tacaaacgta cgcgacagtt
     1621 accgcgctgt gatggatact cgccttaacg aaacgatgcg cgtattgacc gttgtgacat
     1681 tgtcactaac aatcccaacc atgatctcag gtgtattcgg catgaatgtg gcactaccag
     1741 tagatctgca tgatggcgct atcttttgga ttgtaatcgt agtaagttta attgctgcgg
     1801 caagtgtgag tgcatatttt ttgaggaaac ggtaaatgtc actcagcctt aatgtcacca
     1861 acgagtattc atatcgcgct cggtcgcgta tcacggtgat gacggtaatc acagtctgtt
     1921 tagtcgcgag tctagcagta attgctatta agcttgcaag gggggtgcaa gagcatgaaa
     1981 cgctcggtat ggacaagagg atactgttga cgattcatca acatgcgaca ccgttccttg
     2041 attggttagc agtgcacacg accgatattg gtagtcctct tgtcgccatc gctatcgcat
     2101 tatgtattgg gttttacctt gcctataaac gtgaggttat tcggttcttt atcattggtg
     2161 gcagtatggt gggttccgca ggtcttgtgt ccatcattaa atcactgatt gagcgcccac
     2221 gcccacagtt atggcatcac ctattatctg aaaaaggatt ttcattcatt agtgggcatg
     2281 ccactatgag tatgacactt gctgcagcga tagtggctat tctttggcgc acaaaatggc
     2341 gctggccagc aattattggc ggtactgtat acgcaagctg ggttggattc tcacgtcttt
     2401 atctaggggt acattatccg acagatattc ttggcggttg ggtggttgct tcggcctggg
     2461 tacttgttgt tacgctgaca attcatcttg tcttcggcga tacgatcagc attccatttg
     2521 caaaaaagtc ttgacataca agttatacaa gtataacatt atatttatgg cttttgaaca
     2581 taatgtcaaa ctagcgggta cggtaactgt tggtcctaaa gggcaggtgg ttattccggc
     2641 agaagtccgc gaacaaatgc aatttgggcc cggtgacaag ctagttgcac tgtatatcaa
     2701 ggacaaaaaa tcagtgctcc ttattaccga agaacaagcc caggcatttg tcactcatat
     2761 gggtgaacag tttacacaat ttaaaaaaga ccttgaggaa ggaaaagaat cataatgttg
     2821 catcacttct ctttgcgaca aaaaattatt gtcatggttg ctgtgatgag cagcctattc
     2881 cttgttgcac tcgaccagac agtcattgct accgcgttgt cggcaatcgt ccgtgaattc
     2941 aagagctact cgagtcttgg ctttattgtg actgcgtaca tgcttacaac gacgatcact
     3001 gtgccactcg ctggtaaatt gtctgacatg tatggtcgcc gaccaatgct aattacgggt
     3061 gtgagtattt ttacgattgg ttcactactc tctgggttgt caccgtcagt tgaatggctg
     3121 atcgcatggc gagccttgca gggtattggt ggcggtatca ttacctcgca ggcctttacg
     3181 atcgtcggtg acctctttac cccgcgtgag cgcggtcgtt ggcagggcat gattggtgcc
     3241 gtgtttggca tgagctcggt aattggtcca ctaattggtg gctggctaac cgacaaacag
     3301 cacatcctcg gccttgtgac cgactggcgc tggacattct tcattaacgt accggttggt
     3361 attattgctg ccatcattat tatggcaaac ctcccgaaca tcaaacatga tgcaaagcac
     3421 tatccagact atgcaggtgc agcaatgatg tctattgtac ttgcatcagt agtacttgca
     3481 gtcgataata cgaaggttat tttcaagttt ctaactgata ctggtgtaga gcttggcctt
     3541 atccaaggta tcctgtggtc aattgttatc ctaggcaccg gtctctttat agctgttgag
     3601 cgccgtgcaa agcaaccgat cctgccactc cgcttcttca aaaagcaggt atacacaaag
     3661 ctcaccgctg tgacacttct ctttggcgcc gcgtttatgg gttcaatctt gtaccttacg
     3721 cagttcaacc agcaggtata tggcgcatct gcatctcaag cggggtatat gctcctaccg
     3781 atggttgcag gtatgatggt atcgtcaatc ggtactggcc aaatagtatc gcgccttggt
     3841 cgctacaaaa tattcatcgt aactggcttt acacttgcga cgctcagcat cctggcgctt
     3901 ctcacgcttg agccttcatc accgtactgg catgaggcag ttattatgac ctttgcaggt
     3961 ctcggccttg gtatggctat gccaatcttg agcctcgctg ttcagaacga attcgagcat
     4021 caggatcttg gtgctgcaac ctcaagtgtc cagctcttcc gtggacttgg ctctaccatt
     4081 ggtacggcaa tcttcagtgg catgcttact gccggtgtac tcgccgcgat aggcaaacca
     4141 actgacatcc cctacataca aacccttcaa agatctccac aatcagcaca gatgctgaat
     4201 ggtaacattg atgcggatgt attgctgcaa atcaatacac agaaggatac gattgctgac
     4261 ggtgcaaaca aggcctttga taaaatcccc gcacctgcgg tacgcgatga gaccaagaag
     4321 tcatttgcag atcagcaaaa taaatatagt gctgaagtca ccgatgcatt tacaaaatca
     4381 ttgcaccaga tcttcatgct cggcgcagcg ttaatggcac tttcactcgt catagtatgc
     4441 tttgtcaaag aacgtccgct gcgcgatagt gtaaatatca caccatcaga gtaacagggg
     4501 taatggagca agtataaatg cctatgcttg ctctataaag cttcattttg atacagttac
     4561 gtttggctca aagaaatgtc taactacaga gtaatcaaag gtcgagaatt ttcagtaaat
     4621 gagccaaagg taaataaagc acatgccaat agcaatcacg tttgctccgt cgcggagcaa
     4681 aaagatcgcg gtggatgtgg tgcctgccaa ctggcagctg tatatcgcac acgactagcg
     4741 tatatacgct acaaccgctc ttatataaag ggcaaacaac aaacaataca ccccaaggca
     4801 ccggggtgta ttgttttgta ttacgcccga ttactgagaa agcattccgc tgtcttggag
     4861 tactggttta ataacaagcg tgtcaaatgc tgtagcgtca ctccacaccg gttgagcgcc
     4921 agtgacactc tggggaacaa tcttgtcatc cttgaagtac tcactgctta cttctttgcc
     4981 attcaaataa agtgttggcg taccgctcac accttgtttc ttgccaagtg ccatatcaaa
     5041 gttaatcttt ttgctaatat gatttgaatc acttgagtca ccgttaagcg ccgattcaag
     5101 tttactttta tcgataccaa cagcatcagc aaggcctaca aagtagttgg tgcggtctga
     5161 gccgcttaat tgattccatg aatcttgcgt ttcgtatagc ttattgtgca tctcccaaaa
     5221 cttaccctga agatttgctt cttcggcaac ggttgctgca gcaagtgcat tagggtgaag
     5281 cgttgaaagc gggtagttgc ggaagatgaa tccgagctga tctttgtact tttcggtgat
     5341 ttgcttaatg acagggtagg catcaccgca acctgggcac tggtagtcac catactcaac
     5401 caaagtcacc ttgcttttcg tgttaccaaa cgtatggtcg gcaatcttac cgttgtcatc
     5461 cattgcactc tgaattttca atgcatcaac ctttgcgaca tcaaccttat tcatactcga
     5521 catatagata agtccgccaa tgagcgccac gcaaataaca gcaaatataa tccacgctct
     5581 ttttgtcata tacagaattc tccctcgttt atctatataa aagtataccg cgtattgcat
     5641 agcgcagcaa ggtaaaggtg aggtacaata aagccatgca gacgttcatt gtcatacccc
     5701 tctgtcttac catagtcttc ttaattttgg cagcaagcat atctcccagg cgttcgcgcc
     5761 tatcacgatt tgaactcgaa cgtcgtcgcg aagaaggcga cacttcagcg catggcgagc
     5821 ttgagcgtga gttggcaatt gctgacgttg tttcgttcct tcgtgcagtg gttgcgcttc
     5881 ttttggtatt tgccgtatta ttggctgttt cgacctttgg cccgttcttt ggccttatca
     5941 ttggcgtcat acttgcactt gggtatgggc gtatggcaca agttgactgg gtacacggtc
     6001 tagcggaccg catgtaccag ccgtatgatg aatcacttgt cagctttgtg caaacacagc
     6061 cctggattgg t
//
LOCUS       DATIIX010000102         6498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_275424, whole genome shotgun sequence.
ACCESSION   DATIIX010000102 DATIIX010000000
VERSION     DATIIX010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6498
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275424"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VLF64_02855"
     CDS             complement(<1..708)
                     /locus_tag="VLF64_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HSW90912.1"
                     /translation="MNTIFKRTKILATLGPATSSEEKITELIDAGVNGFRFNFSHGSH
                     EERLDQLKWIREASAASSRSVAVLQDLQGPKIRLGVLNDNRHDVKKGDEIILDFAADH
                     HDNILPVQYNLAEKVKVNEPIYLFDGKVRTTVIGIESGTAIRLRIENDGFLMSKKGIN
                     LPDTDFGGDILTEKDLKDLEFGATQDYDYVALSFVQSEDDINNLRQILASHGSTAQII
                     AKVETKAAIRPETLEAIV"
     gene            complement(735..1496)
                     /locus_tag="VLF64_02860"
     CDS             complement(735..1496)
                     /locus_tag="VLF64_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HSW90913.1"
                     /translation="MLLIAIKKDDIITDTMAEYDKTKYELPSDMANNSKLNRLRAAVL
                     GGNDGIVSISSLMMGVAGATSDTHSIALAGLAALIAGALSMAVGEYVSVSSQSDAEKA
                     YVALEKEDLHKDPAGELDELAREYQKLGVSAQTARQVAVELTERDALRAHLHVHFNLD
                     PDDLNSPWQAAIASFLSFSMGGLVPFLAMILTPSAWRIPFAVTAVVIALLAIGYVSAI
                     VGKAPKVRAIIRVVAGGLVAMAITYGVGVLFGHSV"
     gene            1612..3624
                     /locus_tag="VLF64_02865"
     CDS             1612..3624
                     /locus_tag="VLF64_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90914.1"
                     /translation="MFNQVNRRLTAALMALAVVISNVIAPVAPAFASSNDKQVYCNIE
                     GNSGQWKAQISNGNTEHEFKLPGVFVHDQHDVTPAMNDQCTQLFQAPTPTAPVATPAA
                     CDTAGSLTLTDTLDYTWSTNPAYHGPGTYAATATIRNNVNKVFSNGKTSISFNNVIVQ
                     QQLDGPQCHKPADKITYTEWVDGQEDCTTLTVTQTRNKITTPYKWDQNSNSWVLDTAH
                     EVITQDTPRQRNLTSSELMTCTPKPKDTTTYSEWSDTAPYKCGNTFIDQTRTKTSTTY
                     TWSGAAWVSTVTTTTESRTRVPTANELAICAKPADYTTTTTKTATICLPGGGGKTTTW
                     TTVNTYTHILISNKWVSILKSSKTTDQTVDASEADCPTPKQVNPTCNTLGSLTLPAYK
                     DGIPGVHHTYYVWVNGIRTSYSASSDVVVANIPQGATVEVKLYINGTERFGWPIYKHT
                     FTFSTSDCIAIPQPPQQADECGPNNAYWIIPADTNDVHWSTNTTNGHLIATAIGGHFV
                     GGAATIDFGAAKDSNDVCPPAVVTPVKPQIVDCEVVLPITLGITYKEAYLDTNGNIVD
                     PSQAGITATHRGVLATANPGYVLSTTNYDEQGYLYTFDFTDMNCGKGDVTPPTVTPPV
                     VTAASTTPPTPAELPHTGSSLGGLLVTLLATLSTYGVVYFLQPKRR"
     gene            complement(3773..4366)
                     /locus_tag="VLF64_02870"
     CDS             complement(3773..4366)
                     /locus_tag="VLF64_02870"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HSW90915.1"
                     /translation="MARRIVFSIIAVGLLAAAAPTLGLAFNRPAQSIARETSHTPVLS
                     QKSVNDIGIPIRLRIPAINVNSAVEPVGITPGGVMDIKENQDNTAWYKYGPRPGENGN
                     AVIAGHYGWDHQKPSVFTQLSKLQEGDKLYVDSDKRTTIAFVIRKSKRYNPDADTTAI
                     FKSNDGNSHLNLITCDGSWDEAKKSYTNRLVVFADRT"
     gene            complement(4412..5323)
                     /locus_tag="VLF64_02875"
     CDS             complement(4412..5323)
                     /locus_tag="VLF64_02875"
                     /inference="COORDINATES: protein motif:HMM:NF023425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050825 - ice binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HSW90916.1"
                     /translation="MLLIGASGTVMTSSIAFAAQPTVDLQTAAPFAILAGTPNITDAG
                     NTSVITGDVGLSPATGAGIGLLCSQVTGTIYSVDAAGPLPCRVTNGPLLVTAKNNLTT
                     AYTDAAGRTPVTTVPTELGGTTKTAGVYDSASTTFGITAGAGALILDGQGDPSSVFIF
                     EMNAAGTGLTVGAGSTVSLINGAQACNVFWRLDTASVNTTAVFKGSILALNSISVANG
                     ANIEGRLLARNGNVTLINDVIAKPTCTAPSGGGGSGSSTTPKLPNTGVHTEDTMSTVL
                     ILGSVGLITASLCGGYFVEKRHLSAKN"
BASE COUNT         1759 a         1575 c         1403 g         1761 t
ORIGIN      
        1 tacgattgct tcaagtgttt ctgggcgaat tgccgctttt gtttcaacct ttgcgataat
       61 ctgcgctgtt gaaccatggc ttgccaggat ttgacgaagg ttattaatgt cgtcttcact
      121 ctgaacgaag ctaagtgcta catagtcata gtcctgtgtt gcgccaaact caagatcttt
      181 aagatccttt tcagtaagaa tgtcgccacc aaagtcagta tctggcaggt taatcccttt
      241 tttactcatc aggaagccgt cattttcaat gcgaaggcgg atagcagtac ctgattcaat
      301 cccaatgact gtggtacgaa ccttaccgtc aaagaggtag attggttcgt taacctttac
      361 tttttcagca aggttgtact gtacaggcaa aatattgtcg tgatgatcgg ctgcgaaatc
      421 aaggataatt tcatcacctt tctttacgtc atggcggttg tcgttaagaa caccaagacg
      481 aatttttggg ccttgcaaat cttgaagcac agcaactgag cgactactcg ctgcgcttgc
      541 ttcgcgaatc catttgagct gatcgagtcg ctcttcgtgc gagccgtgac taaagttgaa
      601 tcgaaagcca tttacaccag catcgattaa ctcagtgatt ttctcctcgc ttgatgtagc
      661 cgggcccaag gttgccagga ttttagtgcg tttaaagatt gtgttcattt ctataattgt
      721 acaataccga gacactatac gctatggcca aacagaacac ctaccccata ggtaattgcc
      781 atggcaacga ggccacctgc aacaacacgt ataatcgcgc gcaccttcgg tgctttacca
      841 acaatggcgc tcacataccc tattgctaag agggcaatca caacagctgt gaccgcaaac
      901 ggaatgcgcc aagcgctagg cgtgagaatc atcgccagaa aggggactaa gccgcccata
      961 gagaaggata aaaagcttgc gattgctgct tgccacgggc tatttaaatc atcagggtca
     1021 agattaaagt gtacatgcag gtgcgcgcgc aaggcatcgc gctcggtcag ttcaactgcg
     1081 acctgtcggg ctgtttgagc gcttacccct agcttttggt attcgcgtgc caactcatca
     1141 agctcgcccg ctgggtcttt atgtaaatct tctttttcta atgctacgta ggctttttcg
     1201 gcatctgatt gactcgagac gcttacatat tcgcctacgg ccatacttaa ggctcctgca
     1261 attagtgccg cgagtccagc tagggcaatt gaatgggtgt cactcgtcgc tccggcgacg
     1321 cccatcataa ggcttgagat actgacaatg ccatcattgc cgccaagaac agcagcacga
     1381 agtctattta acttgctatt attggccata tctgacggca actcgtattt tgttttatca
     1441 tactctgcca tggtatctgt tattatatca tctttcttga tggcaatgag taacatctac
     1501 acccccgtaa agcataaggg ttttatactt atagaatata attgacgtat accaaacttg
     1561 gtgttacttt aaagtcaata ggaactgtag gagctaaatc ggggaggatt tatgttcaac
     1621 caagtaaacc gcaggttaac tgcggcattg atggctttgg ccgtcgtaat atcaaatgta
     1681 atcgcccctg ttgcacctgc attcgcaagc agtaatgata aacaggtcta ctgtaatatc
     1741 gaggggaatt ctgggcagtg gaaggcgcag attagtaatg gcaacactga gcatgagttt
     1801 aaacttcctg gtgtattcgt gcatgaccag catgacgtta cacccgccat gaacgatcag
     1861 tgtacgcagt tgtttcaagc gccaacgcca acagctccag ttgccacgcc tgcagcatgc
     1921 gacacggcag gtagcctcac gctcacagac accttagatt acacatggtc aacaaatcca
     1981 gcatatcacg gccctggtac atacgcagcg actgcgacta tcaggaacaa tgttaataaa
     2041 gtcttcagca acggaaagac atctatttct ttcaataacg tcattgtcca gcaacaactt
     2101 gatggccctc aatgccacaa gcctgcagat aaaatcacct acactgaatg ggttgacggg
     2161 caggaggatt gtactacttt aacagtcacg caaactcgca acaaaatcac tacaccatac
     2221 aaatgggatc aaaattctaa tagctgggta ctggatactg cacatgaggt tatcactcaa
     2281 gatactcctc gacagcgtaa tcttacttca tctgaactta tgacgtgtac gccaaagcca
     2341 aaagatacca ctacctatag cgaatggtct gataccgccc cttacaaatg tggcaatacg
     2401 ttcatcgatc agacccgtac caaaacgagc acaacctaca catggagcgg tgcggcttgg
     2461 gtgtctaccg taactaccac gactgagagt aggacgcgtg ttcctacagc caacgaactg
     2521 gcaatttgcg ctaaaccagc cgattacaca accaccacga caaaaacggc aactatctgt
     2581 ttacctgggg gtggcggtaa aacaactacc tggactacgg tcaacactta tacccacata
     2641 cttatctcca ataagtgggt aagcatactt aagtcatcga aaacaaccga ccaaaccgta
     2701 gatgcttctg aagcagactg ccctacccct aagcaagtta acccgacttg caacacgctt
     2761 ggcagcctta ctctccctgc atacaaagat ggcattcctg gcgttcatca cacctactat
     2821 gtatgggtaa acggcattag gacaagttac tcagcaagca gcgacgttgt cgttgcaaac
     2881 attccacagg gtgcaactgt agaagtaaag ctctacatca atggcactga aaggtttggc
     2941 tggccaatct acaaacacac atttacattc agcacatcag actgtatcgc tattccacag
     3001 ccaccgcagc aggctgatga atgtggcccg aacaacgcat actggattat acctgcagac
     3061 actaatgacg ttcactggtc tacaaacacg accaatggtc accttatcgc aaccgctatc
     3121 ggtggacact tcgtaggcgg cgctgcaact atcgactttg gtgcagccaa ggattcaaac
     3181 gatgtgtgcc caccagccgt tgttactccg gtaaagccac agattgttga ctgcgaagtc
     3241 gttctcccaa tcacactcgg tattacttac aaagaagcgt acctcgacac taacggcaac
     3301 attgttgacc ctagccaggc aggtatcact gcaacacacc gaggtgtact tgcaaccgca
     3361 aatccaggtt atgtactcag taccaccaac tacgatgagc agggctacct ctacacgttt
     3421 gatttcactg atatgaactg tggtaagggt gacgttacgc caccaacggt aactccacca
     3481 gtagttactg ctgcctctac cacaccccct acaccagctg aattaccaca cactggttca
     3541 tcactcggtg gattgctcgt aacactcctt gcgactctct caacctacgg tgtcgtgtac
     3601 ttcttgcaac caaagcgccg ctagataata gcaaccaaaa ataccccgga ttaaccgggg
     3661 tatttaacgt agtggttcca tatagtaata cccgacaatg ggactagttt gtatttggta
     3721 tcctgaagcg gacacctctt gtataattca ttgctcatgc cgttattgta ttctatgtac
     3781 gatcagcgaa tacaacaagt cgatttgtat agcttttttt agcttcatcc cagctgccat
     3841 cacaggttat tagattcaaa tgagaattgc catcgtttga cttgaagata gcggttgtat
     3901 cagcatctgg gttgtatctt ttactcttac gtatgacaaa tgcaatggtt gttctcttat
     3961 cgctatcgac atacaatttg tcaccttcct gtaacttaga taattgagtg aagacagagg
     4021 gtttctggtg atcccaaccg tagtggcctg caataacggc attgccattc tcgcccggac
     4081 gtggaccata tttataccat gctgtattgt cttggttctc ctttatatcc attactccgc
     4141 ccggagtaat gccaacaggt tcaacggcag aattaacatt aattgcaggg attctgaggc
     4201 gtatcggtat cccaatatca ttaacacttt tttgagatag tactggggta tgagacgttt
     4261 ctcttgcaat actctgggct ggtcgattga atgcgagacc cagggtaggt gcagctgctg
     4321 caagaaggcc tacggctata atactgaata caatacgcct agccatggac ttcgattgtt
     4381 tttcaggcat tgtgagcaag cagatggctc cttagttttt ggctgagaga tgccgctttt
     4441 caacgaagta gcctccacat aggctagcgg taatgaggcc aacagatccg agtattaata
     4501 cggtactcat tgtgtcttct gtgtgcacgc ctgtgtttgg gagcttgggc gtcgttgaac
     4561 tcccgcttcc accgcccccc gatggggctg tacaagtagg tttcgcaatg acatcattaa
     4621 tcagcgtaac attgccattt cgtgccagta gtctaccctc gatgtttgca ccattggcca
     4681 cgctgattga attaagggcg agaatgctcc ctttgaacac ggccgtagta ttaacgctag
     4741 ctgtatctaa tcgccaaaag acattacaag cctgtgctcc attgattaat gataccgtac
     4801 tcccagcccc aacggtcagt ccagtaccgg ctgcgttcat ttcaaaaatg aaaacagagg
     4861 aaggatcgcc ttgcccatca aggataaggg cacccgcacc tgccgtaatt ccaaaagtgg
     4921 tagatgctga gtcatatacg cccgccgtct tggtggtacc acctagctcg gttggtacgg
     4981 ttgttacggg agtgcgcccc gccgcatccg tataggctgt cgtcagatta tttttggctg
     5041 tcaccagcaa cgggccgtta gtaactctac aaggaagcgg ccctgcggcg tccacggaat
     5101 agatcgttcc ggttacttga gaacaaagta gcccaatccc agcacctgta gctggactta
     5161 gtccgacgtc tcctgtaatg actgatgtgt tacccgcgtc cgtaatgttg ggagttcctg
     5221 caagaattgc aaatggtgcg gctgtttgta aatctacggt aggctgcgct gcaaatgcaa
     5281 tagacgaagt cattaccgtt ccactagctc cgatcagtaa cattatgccg atcaaaattt
     5341 ttcttgaatt tttataagtt ttcatgtcct attccctatt gttatttgac gaacgataaa
     5401 cttgaaccta tgcaaaggtt ttttatatgg acgatttaat cgaagacgta ggatcatccg
     5461 agttcttttc tagaatatgt acagaattat ttttcctcat ctcgcgagga gtgatgttaa
     5521 atacatcctt gaagacaatg ttgaactgtc ggatgctaga gaaaccaaca ctaaatgcaa
     5581 tatcagtgat tggtaacttt gtacacgtta tgagttgttt agctgcgtca atacgtttct
     5641 cctgattaaa atgtaatggt gaagaacccg ttactcgtcg gatgatgcgt cgcagctgtc
     5701 gttcactcat atgtaacgta tctgcgaagc catttacgcc acgatcattt ataacgccgg
     5761 catctatgct atcgagtatc actagtgaca ggtcaccctt tgatgtatct agacagcaac
     5821 gtccacatgt ctgatatccg catgagattg cctccgaagc agtgtcgaag aaggtgtatt
     5881 ctgcctgcaa tggatgagga gctggacaag agggacgaca atatgttcct gttgtttttg
     5941 caaaataaaa cacgccatca aacttcctgt ttctttctcg cactgcgcga taccgtacgg
     6001 agttgcttaa gctcgtcgtc atatgtacat ctttgcatgg taagttttta tatactagcg
     6061 attatcggac attcaaagat atcctttttg cgcaaagagc tatccctcca ctaaattatt
     6121 aaccaatatt tttctccgaa ctttgactat gtagtatctt tattaagatc acacttagtg
     6181 ttttgtgaat tgtgatgaaa taatcaagaa actaaaatgc aatagatggg tcagctgcaa
     6241 ttggtgatca aaatataata cttcctcgct cgaaaaaacg aacaagctcg tttttctctc
     6301 gcctcgtcgt attatcgtac ccggattatg ttttgcttgc gcaaagccaa tccgaatccg
     6361 actactcgca aaagcaccaa gttttaaacc ccacacaagg tgaggtgtta aacttggtga
     6421 acctaagggt gaatagggtt gaaatcttct tcggtgagtt ggaatatctt actactcacc
     6481 tatgcagcta aatatatg
//
LOCUS       DATIIX010000103        10620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_525800, whole genome shotgun sequence.
ACCESSION   DATIIX010000103 DATIIX010000000
VERSION     DATIIX010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 10620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10620
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525800"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VLF64_02880"
     CDS             complement(<1..277)
                     /locus_tag="VLF64_02880"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HSW90917.1"
                     /translation="MNILTFVTFCVAFLSSVISGMSGGGGGFIMTPYYLFIGLSPQQI
                     AAGASVASLGLSGSSLIAMRDKQLIDKRFLWPLVAVTIIATLLAMIAL"
     gene            complement(549..2261)
                     /locus_tag="VLF64_02885"
     CDS             complement(549..2261)
                     /locus_tag="VLF64_02885"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HSW90918.1"
                     /translation="MARLPVPGSDNEIWGNILNEFLSVEHNTDGTLKSTGSLATKAND
                     AAVVHNAGNEMIGGIKTFSTAPVVPSNSFPESAITNLTADLNARLTFRGAWAPSTAYV
                     THDLVTFSGTSYVAISSFTSGTSFSLTNWTPLMAQPGVFNVKDYGAKGDGVTDDTVAI
                     TNTINAAYAAGTADGTNYAEVYFPPAVYLIAGAPTTGGATFGSAQIPLPVTTPEDPVT
                     HHIPQQKFILVLRGTVSATALYHWNQTVPQEAGAVLRSTYDAGTTLPGTGEVSVIGGP
                     TPHFGYGNGTSTFDNMLVTVSGITVEVPQNGNICGFDFRGMAEANILNASVLARSTVT
                     GAPLVPDPNWAFGLAMPQTNNNDICNIGYFSCEGLVYGLIIYEHVQANSLQLINCFDG
                     LVIWPSSGAPHRNIITHASIENCNQCVVFASTGVTKLDILVLDIEWTTGAGVHSIVND
                     THNAAYGTIYVGSNGGDGGITLQEALSASYGVIGGSHMKVVNVDQSVGAIAAPAMPAS
                     TTTLQNPFWRDAAITITGGTVTDITVDGTTQLTATPGTIYLPSGRSITITYSVAPSWS
                     WILL"
     gene            complement(2371..3387)
                     /locus_tag="VLF64_02890"
     CDS             complement(2371..3387)
                     /locus_tag="VLF64_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90919.1"
                     /translation="MNKVNPTSHTRDIAVFLQSIYVRGFHGEQIRHAKEAVGLGVENS
                     KLSKQQLNTLAKYLNKLDQPYQTQGVDFSRAQISLLMNARTAGYPIEFETFGPYLTDY
                     LQYEGNMHASHLIPEDSIGLSLAGVMRRFFPSARLISLYDEYNVKKGTVHRSEELAHF
                     TRRHKENFRASLLALLQYAAIVPEDAVDGKDYLLIPESSRVPDADHLVTQLESRGFIF
                     RRGDEIVFVNEDAENPLYQRVILRTRQGRWLCEALDAATFLKPENQKITHLVMLPDYM
                     KAQQDKVWEILRTLNIPRAHYHNIFYNPTMLPQTAVRVVTEVFETAEMFFADKDSIYS
                     PESL"
     gene            complement(3514..3924)
                     /locus_tag="VLF64_02895"
     CDS             complement(3514..3924)
                     /locus_tag="VLF64_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90920.1"
                     /translation="MNVLVKRFLKVAVIPMAVVMFVVGCKSTTKNADQGAQGQTQNVC
                     DVLKPLPSGSGPIPSPDAQQLSCLNNVRQTIGTLSEDNKVKPLMPERLKECKDALVTW
                     HAWTDRQSDAWFTANNMSRKLPGDCGMQPTPTKR"
     gene            4251..5270
                     /locus_tag="VLF64_02900"
     CDS             4251..5270
                     /locus_tag="VLF64_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90921.1"
                     /translation="MAYNATREALEVTDTTVRRYFLAAIFHGMLVAKPTPDQIVGMLE
                     ASFSFDDYHPNTRPMLAPKTNKTPTITLMGSGKKGLKTVNISTLAAITASSSKRIIVT
                     KPCTEAISSLTGSADFLQVLGIEAHKDKDQMRDIALKTGLGLFFVNSYMPQFMERYDG
                     VFYAPHAMSYALAGLVSPFSTDSLLYGLALEDTALSYEVFKRYNITNPTVVSSTVDGV
                     HFVDEALLVGKISISTATNNGVYEFSKIIGGSPIKIESFEPAKSATKKDNVLKGLRSI
                     LPGSHSDLSRTIALNAGLIMYAGGVVDSIEHGYDIAMALIADGSAWKQLHEFVEDVEL
                     LQTIV"
     gene            complement(5706..5936)
                     /locus_tag="VLF64_02905"
     CDS             complement(5706..5936)
                     /locus_tag="VLF64_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90922.1"
                     /translation="MVAVVLRQRYQTDPAGTIRQINHLMAAGSKATLTDIYEAIDITL
                     GPDLWRECISAIADQVDQATALAQQLGLIPAT"
     gene            complement(6123..6626)
                     /locus_tag="VLF64_02910"
     CDS             complement(6123..6626)
                     /locus_tag="VLF64_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90923.1"
                     /translation="MDGFDDLDIESTQPLDMNHDTLQPGSKATPMSRLIYGVAASDIS
                     AIEDQIADNIEWGLMPYLKVLKGKEQVMSWLKAGSSDQKEPVMITNAMAKDWGVFEYW
                     NIGTLSDDVIEFGNANHWPWPKDPKSLLGQKYKVVQCFVYHVNAAGKIDVMRQYLDTQ
                     SLWAQLK"
     gene            6844..7419
                     /locus_tag="VLF64_02915"
     CDS             6844..7419
                     /locus_tag="VLF64_02915"
                     /inference="COORDINATES: protein motif:HMM:NF037625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HSW90924.1"
                     /translation="MQHKMGLRQQKKQHVLNTVHKTAVTLFTKRSYDTVSMEDIAKAS
                     GISRSTLFRHFATKEATVLHNNLNPILRDIFRNQPTHITAIQAIRNTLRIVPLQSNAS
                     KLHEQRYNLIRTVPQLRIALLQELAQTSDMLIDLIAERTNRSTDDIVVCTLANALMGT
                     AVGIVLSLNETEDYLTRFDQALEQLEAGFVI"
     gene            7619..8779
                     /locus_tag="VLF64_02920"
     CDS             7619..8779
                     /locus_tag="VLF64_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90925.1"
                     /translation="MPHHPDLLARLRVAIPHKASAKDLEILRATGMETCTVLLTAEEK
                     RRRLAGQDAWALDHATQAYFVACWCAFVNQALGAAFLDRSYEINLSAVGYVPQVTADQ
                     TEAFFWEVAEWLPGISQVAVSSPYILGLGIPHELPGWVGAEPCPRAHLLAMFAACATI
                     VEHAEVAVNDLNALGADKHSADMRVLLGDLRAAKTAMIYAADLLPQLNDSSIGQRLHG
                     EIEKNVKLAIGLTYRVGQLAAMPQLIGRSQLTPRRNRSTAKRKLPLPSERGFNKWCLT
                     DPAIHQHWRRDPAAVSAIKMLWQRDPDPEATLDIQSEINAANALGAIWCNGQGNYYCC
                     PWPAIYQALDPITIGGCSILRGQTFTFDVSVKEGRFKREILVASFRPIAEAD"
     gene            9419..10138
                     /locus_tag="VLF64_02925"
     CDS             9419..10138
                     /locus_tag="VLF64_02925"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HSW90926.1"
                     /translation="MDSDDPVIASALHVLSKFKPKSVFLYGSRGRGDAKQDSDYEIGV
                     IFDDDKYVHRSDIHAAITDSQVKAYPFKWGELSSGTFGHVFQKSIYLREIITGGKTIA
                     GERLIEQISLPHITTLDLIQRLRFDIGMALAAILSFRTGDMQTSMEEFSKSCFFGLRC
                     LEILELKTLPVGYEEIYNLSSKLVIDPDYRSVIEAAHKVRNTGEPASIDILFDNISLL
                     DSLIEPKILVAFNTRGNENIL"
     gene            10122..>10620
                     /locus_tag="VLF64_02930"
     CDS             10122..>10620
                     /locus_tag="VLF64_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90927.1"
                     /translation="MRTFYDSTKCSDWRRVGVMTSIRCDIIILPTEGLARGATVMSAE
                     LQKFDTLFQLNTTGLFPHVSLYMVQLKVEDLDRIRASLATIAANAPQFDLTATRYSQA
                     KGYIDVEYARTENLDKLQMTIIDAISPIRDDMREKDSARVSAATGRVRENLEKYGYHA
                     AGELFR"
BASE COUNT         2714 a         2622 c         2537 g         2747 t
ORIGIN      
        1 gtagggcgat catcgccagc agcgtcgcga ttatcgttac tgctacgagt ggccacaaga
       61 accgcttgtc tatgagctgc ttatcgcgca ttgcgatcag tgagcttcca ctgagtccga
      121 gagacgctac ggacgctcca gctgcaattt gttgcggtga taggccaata aaaaggtagt
      181 atggcgtcat aataaagccg ccgcctccac cgctcatgcc actaataacg cttgataaaa
      241 acgcgacaca aaatgtcacg aaagtaagta tgttcattct atttccttta aatgataatt
      301 cagtttttat atatgaagaa attgatgcag cgcgtcgctg acataatcta tgccaaaact
      361 gtacaacttt ttttgagaga acagtgggta actgctattg tgattaacat tgtaccacag
      421 gataagtgag tagtcaatag cagtttcctt gtttcgataa gtgctatctc aaactctcca
      481 aagcaaaagc aatgaaaaga ccccatcgca gctgcgatgg ggtcttgatg tttctttcta
      541 gctatacttt acagtaatat ccacgaccaa gatggtgcta cagaataagt tatggtaatc
      601 gaacgacctg atggtaagta tatcgttccg ggtgttgcag tgagctgcgt cgtaccgtcg
      661 accgtgatat ctgttaccgt accacccgtt attgtaatag ccgcatcgcg ccaaaaagga
      721 ttttgcaagg tcgttgttga cgcgggcatg gcaggtgcgg cgatggcgcc gactgattga
      781 tccacattga caaccttcat atggctaccg ccaattactc catacgacgc acttaatgcc
      841 tcttgcagtg taatgccacc gtcaccacca ttactgccaa cataaatagt gccgtatgct
      901 gcgttgtgcg tatcattaac aatgctatgg acacccgcac cggttgtcca ttcaatatcg
      961 agcaccaaaa tatcgagttt agtgacacct gtacttgcaa atacaacgca ctggttgcag
     1021 ttttctattg aagcgtgggt aataatgttg cgatgcggag cgccagatga cggccagata
     1081 acaagcccgt caaagcagtt gataagttgc agcgagttag cctggacatg ctcgtaaatg
     1141 ataaggccgt atacaagccc ttcacacgag aagtagccga tgttgcagat atcattattg
     1201 ttcgtttggg gcatagcgag accgaacgcc cagttaggat ctggaacgag tggtgcgccc
     1261 gtaacggttg aacgtgcaag aacggatgcg tttaatatat tagcctcagc cataccgcgg
     1321 aagtcaaatc cacagatgtt accgttttgg ggaacctcaa cagttatccc gcttacggtc
     1381 acgagcatat tatcaaacgt tgaggtaccg tttccgtatc cgaaatgagg ggtaggtccg
     1441 ccaataaccg atacttcgcc agtcccaggc aaagtagtcc cggcatcgta ggtagatcgc
     1501 agtaccgcgc ctgcctcttg tggtacggtt tggttccagt ggtaaagcgc cgttgcacta
     1561 actgtacctc ttaagacaag aatgaatttt tgttgtggaa tatgatgcgt tacaggatct
     1621 tcaggcgtgg tcactggaag gggaatttga gcgcttccga atgtggcccc accagtggtt
     1681 ggtgcacctg caatcaaata gactgcaggt gggaaataga cctcggcgta gttagtgcca
     1741 tctgcagtgc cagccgcata cgcagcattg atggtattgg tgatcgcgac cgtgtcgtcc
     1801 gtaacgccat ctccttttgc tccgtagtct ttcacattaa agacgccagg ctgcgccata
     1861 agaggagtcc agtttgttaa actgaagctc gtaccacttg taaacgaaga tattgcgacg
     1921 tagcttgttc ccgaaaacgt gacaagatca tgggtaacgt acgctgtaga tggcgcccaa
     1981 gcacccctaa aggtaagtcg tgcattaaga tcggctgtta gattagtaat tgcgctttcc
     2041 ggaaaagagt tactcggcac gactggcgca gtagaaaatg tttttatacc accaatcatc
     2101 tcattacctg cattgtgaac aaccgctgcg tcgttggctt ttgtagcaag actgcctgtt
     2161 gatttcaatg ttccgtcggt attatgttca acactcaaga actcattaag tatattgccc
     2221 catatctcat tatcactgcc tggtactgga agacgcgcca tactgtttac aaacccctta
     2281 ttgctaatac tagtatggta tacaaaatgc ttgtgattgt aaatacttga ggtttctatg
     2341 ccttctcaac gtaccgcaaa atagaccggg tcacaatgat tcaggggagt atatcgaatc
     2401 tttatcggcg aagaacattt cagccgtctc aaacacttca gtgacaacgc gtactgcggt
     2461 ttgaggcaac attgtgggat tatagaaaat attatggtaa tgcgctcgtg gtatgttaag
     2521 tgtccgcaaa atttcccata ccttatcctg ctgtgctttc atataatccg gcagcataac
     2581 aagatgagtg attttttgat tttctggctt taaaaaggtg gcggcatcaa gtgcttcgca
     2641 taaccatcgc ccctgtctcg tccttaagat cacacgctga tatagggggt tttcggcatc
     2701 ctcgtttaca aatacgattt catcaccacg ccgaaaaata aaaccacgcg attcaagttg
     2761 cgtaacaaga tgatcggcat ctgggactct tgagctttca ggaattaaaa ggtaatcttt
     2821 tccgtcaacg gcatcctcag gtacaattgc cgcatattgc aaaagcgcaa gtaaactcgc
     2881 acgaaagttt tctttatgcc gccttgtaaa gtgggctagc tcttcacttc tatgaacggt
     2941 acccttctta acattatatt catcatagag ggagatgagg cgagctgacg gaaagaatct
     3001 gcgcattacg ccggctagac taagcccaat gctatcttcg ggaataagat gggacgcgtg
     3061 catgttgcct tcgtactgta agtaatccgt aagataggga ccaaacgttt caaattcaat
     3121 agggtagccc gcagtgcggg cattcataag caggctaatc tgcgcccgcg aaaaatcaac
     3181 accttgagtc tggtaaggct gatcgagttt atttaaatat tttgccagtg tattgagttg
     3241 ttgctttgac agtttactat tctcaacgcc aagtcctacc gcttcctttg catgtctaat
     3301 ctgttcgccg tgaaagcccc gaacataaat agactgcaaa aatacagcga tgtctcgtgt
     3361 atggctagta ggattaactt tattcatgtg ttatatcgaa taactgctcc tactataata
     3421 tctgtttttc ctaaaataaa taagtctaca tattatacat cggcaggggc ggggcgtcgt
     3481 tgcccctgcc cctgccttca ttacgatgcg gatctaccgc ttcgtcggcg tgggctgcat
     3541 cccacagtcg ccagggagtt tgcgcgacat gttgttggcg gtgaaccagg cgtcactctg
     3601 acggtcggtc cacgcatgcc aggtgacgag tgcgtctttg cactctttca agcgctccgg
     3661 catgagcggc ttcaccttgt tgtcctcact gagcgtgccg atcgtctggc gcacgttatt
     3721 gagacagctc agctgctgag catccggtga cggtatcggc ccagagccgg atggaagggg
     3781 cttgagcacg tcgcagacat tctgtgtctg cccctgcgcc ccttgatctg cgtttttcgt
     3841 tgttgatttg caaccaacaa cgaacattac gacagccatt ggaatgaccg ccaccttcag
     3901 gaacctcttg accaagacgt tcattaccat ctctcttcca agtcaagatc gcgcacggcg
     3961 cacgcagttt ggtccttggc cttggaaaaa tagatacatt aaataaatat accgactctt
     4021 tcaaggccaa gtcgtaggga gcaaaagctc gacctacttt ataattatac gcatattatg
     4081 tgtatacaat cttagattat tcaaaaaaac aacagatttg atataccgtg cgctcattta
     4141 tattgcttat ggtaaaatga caggatgaaa aaagtcgaag tagctcagga atacccatat
     4201 tgccgtgagg ccctctatta gttgaatgct caaaacccat cgatcagaag ttggcatata
     4261 acgccactcg tgaagcgttg gaggttacag acaccactgt tagacgatat ttccttgctg
     4321 ccatttttca cggaatgctc gtcgccaagc caacacccga ccaaatcgtt ggtatgcttg
     4381 aagcatcatt ttcgtttgat gattatcacc caaacacccg ccccatgctt gcaccaaaaa
     4441 ccaataaaac gccgacgatt acacttatgg gaagtggtaa aaaaggccta aaaaccgtta
     4501 acatttcaac gctcgcggcc attacggcta gcagctcaaa acggatcatt gttactaaac
     4561 cgtgtactga agcgatatct tcactcacag gttcggccga tttcctgcaa gttctcggta
     4621 ttgaagctca caaagacaaa gaccaaatgc gtgatattgc tctaaaaaca gggctcgggc
     4681 tgttttttgt taattcgtac atgcctcagt ttatggaacg atatgatggt gtattttatg
     4741 ctccgcacgc tatgagttat gcgctcgctg gtctcgtctc accttttagt acggattcgc
     4801 ttttatatgg acttgcactt gaagatactg cgttatccta cgaagtattt aagcgctata
     4861 acattactaa tccaacagtc gtgtctagca cggttgacgg agttcatttt gttgatgagg
     4921 ccctgctagt tggcaaaata tccattagta cagcgacaaa taatggcgtc tacgaattta
     4981 gcaaaataat aggcggcagc ccgattaaga ttgaaagctt cgagcctgca aaaagcgcca
     5041 ctaaaaagga taatgtgtta aaaggacttc ggtctattct acctggatca cattccgacc
     5101 ttagtcgcac gattgctctt aacgcagggc tgattatgta tgccggaggg gtagtcgata
     5161 gcattgagca cggctatgat attgccatgg ctcttattgc ggatggaagc gcctggaaac
     5221 agcttcacga attcgttgag gatgttgagc tattgcagac aatcgtctag agaatgtcac
     5281 acctcaatca tccatatctc acgacgagcg tatcactcta ctgaggtatg gaatttgcct
     5341 acgatcaaaa aatcagccca cgcgccgcga taatacggca cgtgggcgag gtggcgggct
     5401 gtatgccaaa gggtaatcaa tcttggttag atagtttacc gtttttgctt tggctctttt
     5461 ttgcccgagg gttctttagg accaggtttg cgcgatggct tgggcgaagg ctttttgggg
     5521 ccaccagttg gcgccgcagc caatgtcata cacgagtgca tcgtaacctc cattgaatcc
     5581 tataactgcg acaaagcgcc gaagtcatag taataagtat accagtctta cattaactac
     5641 cccgccaacc actcggtcgg cggggtagtt gccgtaccat gagtagttga cggggttgcg
     5701 cgctgctatg tggccgggat cagacccagc tgctgcgcga gcgcggtcgc ctggtccacc
     5761 tggtcggcga tagcgctgat gcactctcgc cacaggtccg ggcccagggt gatgtcgatc
     5821 gcctcgtaga tgtcggtcag cgtggccttg ctgccagccg ccatcaggtg attgatctgt
     5881 cgaattgtgc cggccgggtc ggtctggtac cgctgtcgta gcacaacggc gaccataccg
     5941 gctaggacat agccgtagtt gtagaacccc tggcgcagct gcggccagta gatcctatgc
     6001 tgtttacctg ctatgcgggg atagttgctc tctaaagttt agacaacacc ctctctctag
     6061 ttatgaaaaa ttaaatagtc tgcagtttaa actgcagact atttaatttt caacaaattt
     6121 agctacttaa gctgcgccca caggctttgt gtgtctaagt attgcctcat aacgtcaatc
     6181 ttacctgctg cattgacatg gtagacaaaa cactgcacca ctttgtattt ttgccctagg
     6241 aggctcttag ggtcctttgg ccaaggccag tgattggcgt tgccaaattc aattacatca
     6301 tcactaagcg tgccaatatt ccaatattca aacacgcccc aatccttagc cattgcgttt
     6361 gttatcataa caggctcttt ttggtctgat gacccagcct taagccacga catgacctgt
     6421 tccttacctt taagcacctt aaggtaaggc ataagtcccc attcgatatt atctgcgatc
     6481 tgatcctcga tggccgatat atcactcgcg gcaactccat atatcaacct tgacatcggt
     6541 gtagcctttg agcccggctg cagtgtgtca tggttcatat caagtggctg tgtactttca
     6601 atgtcaagat cgtcgaatcc gtccatatgt tatctccttc tttgatttat ttaataccac
     6661 taataagtat aagcgtcttc aggaggcgct gtcaattatt ttgatactcg gtaccgtttt
     6721 cgattaaaat ccacttcaaa tcacataggt tttcgccgca tgccaataca gcactcaaag
     6781 agaagagcgt aattttggta aagaatgtcg ctaatggcgt caaaataggg tatagttgct
     6841 tgcatgcaac acaaaatggg acttcgtcag caaaagaagc agcatgtact aaatacggtg
     6901 cataaaacgg ccgtaacgct ctttactaag cggagctacg atactgtatc catggaagat
     6961 attgcaaagg cgtcggggat atctcggagc actcttttta ggcattttgc aaccaaagaa
     7021 gcgacggttc tacacaataa cctcaatcct atactccgtg acatatttcg caatcaacca
     7081 actcatataa cagctattca agcgatccgt aatacattgc gcatcgtacc cttacagtcc
     7141 aacgctagca aattgcacga gcaacgctac aacctcatcc ggactgttcc ccaattacgt
     7201 atcgccttat tgcaagagtt agctcaaaca agcgacatgc tcatagatct gattgccgaa
     7261 cgtaccaacc gctcaactga tgatatcgtt gtttgcacac tcgcaaatgc gcttatgggt
     7321 acagcagtgg gtatcgtact tagcttaaat gaaacagagg actatcttac aagatttgat
     7381 caggctttag agcagctcga ggctggattt gtaatatagt gactcattct ttgtcatgga
     7441 ttatctgacg cgctttgtat tactagcaac ccgtaccgct cgagtaagcg cagtagccgt
     7501 tggtaatatt gcgtctgatg ccatattatt ttataattaa tagattggta tctcgtcgtc
     7561 cgccatgcac acgcgaaaga gacggcaatt gtttttgcgg aaggagtagt gacagtttat
     7621 gccacatcac cccgatctgc tcgcgcgact gcgcgtcgcc atacctcata aagcgtccgc
     7681 caaggacctc gagatcttga gggccactgg tatggaaacg tgtactgtac tcctcactgc
     7741 ggaagaaaag cgtaggagac tcgccggcca ggacgcctgg gcactcgacc acgccactca
     7801 ggcatatttt gtggcctgct ggtgtgcatt cgtcaaccag gcactcggtg cggcgtttct
     7861 cgacagaagc tacgagatca accttagtgc tgtcgggtac gttcctcagg tgaccgcgga
     7921 ccaaaccgag gcgttcttct gggaagtcgc cgaatggcta cccgggattt ctcaggtagc
     7981 ggtcagttcg ccttacattc tcggtttggg catcccgcat gaactaccgg gctgggttgg
     8041 cgctgaaccg tgtcccagag cacatttgct cgcgatgttt gccgcctgtg cgacgattgt
     8101 cgagcacgcc gaggtcgccg tcaacgacct caatgcgctg ggcgccgata agcattcggc
     8161 tgacatgcgg gtcctcctgg gcgatctgcg ggcggcaaaa accgccatga tctatgcggc
     8221 tgatctgctg ccgcagctca acgacagctc gatcggccag aggttgcatg gggagattga
     8281 gaagaacgtc aaacttgcca tcggactgac ctaccgcgtg ggccagcttg ccgccatgcc
     8341 gcagctgatc ggcaggtcac agctcacccc ccgccgcaac cggtctacgg ccaagcgaaa
     8401 gctaccgctg cccagtgagc gtgggttcaa taagtggtgc ctgaccgatc ccgcaattca
     8461 ccaacactgg cgccgcgacc cggcggctgt cagtgcaatc aagatgctct ggcagcgcga
     8521 ccccgaccca gaggcgacgc tggacatcca gtccgagatt aacgccgcca acgcgctggg
     8581 cgccatctgg tgcaatggcc agggcaacta ttactgttgt ccgtggcccg cgatctacca
     8641 ggcgctcgac ccgatcacta taggtgggtg cagtatcctc agggggcaga ccttcacatt
     8701 cgacgtctcc gtcaaggagg gaaggttcaa gcgcgagatc ctcgtcgcca gcttccgtcc
     8761 aatcgctgag gcagactagg agggccgaag gcaagcatac gactaacact cgtcaggcac
     8821 cgctgccctc agccacgggt atgtggcaaa gccctggacc ttccaggcgt cagcataggg
     8881 tgggctggct aagtacggtt cgggctgtca tcacgacacg cccaatccga acagccggct
     8941 ccccctgtgc aggatctacc caggatctat tcgggcattt tacagttaca ataatattta
     9001 taagttcatg gcatatccag cccacgtacc attaatcagg tacgcgggct gggcagacta
     9061 aagaatagtc ctaggattcg ccctagttca cgtcaccgcg gcggcggcgt cggctggggt
     9121 ggcgtccgcg gtggcttcgg cctgaccggc ggccggcctc ctggcggagc tgtgggcggt
     9181 ctcggcgtcg gtggtgatgc gaacaacacc attctttccc ttcgtagagt cctgtggcag
     9241 caacggaagt gtcgaagcca actctatata ttataatata ctttctacag tttcatgtat
     9301 gtatgttaca aaaatgtaaa ttttagctcg acatgcgtgg tgagtttcat aaaaagtaaa
     9361 ctcgctttta ctggcagcaa atgcgagtaa ctgctacttg gtgcggtatt atgagagtat
     9421 ggatagtgat gatccggtta tagcgtccgc actacacgtg ctctcgaaat ttaagccaaa
     9481 gtctgtattc ctctacggtt ctcgcggccg cggcgatgcc aaacaagata gtgactatga
     9541 aatcggggtt atctttgacg atgacaaata cgtccaccgc agtgacatac atgccgctat
     9601 tactgactca caggtaaaag cctacccctt caagtggggc gagctatcga gtggcacatt
     9661 cgggcacgtc ttccagaagt cgatttacct gcgtgaaatc atcacgggcg gcaaaacgat
     9721 tgccggggag cgcctcatcg aacagatttc gctaccccat ataactacgc tcgatcttat
     9781 tcagcgctta cgcttcgaca tcggcatggc tcttgcagct atattgtcat tccgtaccgg
     9841 cgatatgcag acgagcatgg aagagtttag taaatcctgc ttctttggtc tgcgctgtct
     9901 agaaatatta gagctgaaga cgctccccgt cggttatgaa gaaatctaca acctgtcttc
     9961 taagctagtc atcgatcctg attaccggtc agttattgag gcggcacata aagtgcgtaa
    10021 cacgggcgaa cctgcaagta tagatatact gtttgataat atctcgctgc ttgatagctt
    10081 gattgagccc aaaattttgg tggcatttaa tacaagagga aatgagaaca ttctatgata
    10141 gcaccaaatg cagtgactgg agaagagtgg gtgtcatgac ttctatccgc tgtgacatca
    10201 tcatcctccc tacagaagga ctagctcggg gtgctactgt aatgagtgct gaactccaga
    10261 aatttgacac actttttcaa ctaaacacga ctgggctatt tcctcacgtt tcactctata
    10321 tggtgcaact taaagttgaa gacctagata gaatacgtgc aagccttgca actattgcag
    10381 ctaacgcacc acaattcgat ctaactgcta cccgctatag tcaagccaaa ggctatattg
    10441 atgtagagta cgcccgtacc gaaaacttag acaaactaca aatgacaatc atcgatgcga
    10501 ttagccctat ccgcgacgat atgcgagaaa aagatagtgc ccgtgtgtcg gccgctacag
    10561 gtagggttcg cgaaaacctc gagaaatatg gctaccacgc tgctggcgaa ttgtttcgcc
//
LOCUS       DATIIX010000104         2813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_192353, whole genome shotgun sequence.
ACCESSION   DATIIX010000104 DATIIX010000000
VERSION     DATIIX010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192353"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VLF64_02935"
     CDS             <1..372
                     /locus_tag="VLF64_02935"
                     /inference="COORDINATES: protein motif:HMM:NF021427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HSW90928.1"
                     /translation="RPQFFIPMITLLRNAALNSLKYKSELAEVREQNIDIANFEDNMN
                     EFKMSFSHRTDLAFDQYAKAIEEIDKSIDRLTKVKDNLMKSGNSLRLANNKAQDLSIK
                     KLTRGNPTMQAKFEEAKRAKQ"
     gene            complement(390..1028)
                     /locus_tag="VLF64_02940"
     CDS             complement(390..1028)
                     /locus_tag="VLF64_02940"
                     /inference="COORDINATES: protein motif:HMM:NF038065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HSW90929.1"
                     /translation="MKQAKNTVNRNTFLISYKLFFALLGFSSIVTEMAVTIDRGTFRP
                     ENFFSYFTIETNILAVIALLLSAVAIAAGKGSKFDLLRSATTVYILVVGIGFSVLLSH
                     MDDVALTAVPWDNVVLHYLIPIAVFADFIMDRPRRKLAFKKALSWLLFPAIYATYALV
                     RGWLTGWYPYPFLNPDISGVESVVATVVGLLALGFVLVWGVGKLSDNSQKAR"
     gene            1111..1278
                     /locus_tag="VLF64_02945"
     CDS             1111..1278
                     /locus_tag="VLF64_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90930.1"
                     /translation="MSKHRRNIFGASGGMFIGMIITGTALGLLLNNVLVGSMLGVSVA
                     LIITTLLRKDE"
     gene            complement(1347..2729)
                     /locus_tag="VLF64_02950"
     CDS             complement(1347..2729)
                     /locus_tag="VLF64_02950"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSW90931.1"
                     /translation="MKEVTYRLVLAIAILASIIAFLDGSIVNVALPAIQHTLGGGLST
                     QQWVVDAYLITLGAFMLVAGSLSDIIGRKRVMMIGLIGFGVTSLLCAAATTKELLIIG
                     RLLQGTTGALLVPSSLALIMSRVPKDRQHAAIGAWTSWTGIAMLIGPFVGGVLIDLGS
                     WRYIFLLNVLPIAVTVYLLARLKDEHDTTTRVRLDWYGAVLGALSLGGIVYALIEQPH
                     FGWAYPLIYGTMAVGVVSFILFIWREKVAAQPMMPLGLFRSRNFSAGNIATLSIYAGL
                     SLVAFLITIFVQQVGHYTATAAGLTMVPMSILMFFLASRFGKLSGEYGPRIFMTVGPL
                     IVAAGFVTMLFIDATANYWAMLPGILLVGIGLSVTVAPLTAAILGAVDSKRSGIASAI
                     NNVVARVAGLVAVALIGTVTGYNLNLAGFRNGLVLAIVLFVVGGFVSLVGIRNPARLK
                     SDPSQAPVVE"
BASE COUNT          897 a          760 c          578 g          578 t
ORIGIN      
        1 cgcccacagt tcttcatccc gatgattacc ttgctacgca acgccgcgct caactcgctc
       61 aaatacaagt cagagctcgc cgaagtgcgc gagcaaaaca tcgatatcgc caacttcgaa
      121 gataacatga acgaattcaa aatgagtttt tcacatagaa cagacttagc ctttgaccaa
      181 tacgctaagg ccattgaaga aatcgataaa tccatcgacc gcctcacaaa agtaaaagac
      241 aaccttatga aatcaggcaa cagcttgcgc cttgccaaca acaaagccca agatctcagc
      301 atcaaaaagc tcactcgcgg caatccaacc atgcaggcaa aattcgaaga ggcaaagcgc
      361 gccaagcaat agcaaaaagc aatcgggaat tatcgagctt tttggctatt atcactcaat
      421 ttgccaacgc cccatacaag cacaaaacca agcgcaagca agccaacaac tgtcgcgacg
      481 actgattcta caccagatat atcaggatta agaaacggat acggatacca gccagtaagc
      541 caaccgcgta ctaatgcgta tgtcgcgtat atggcaggaa agagcaacca cgacaacgcc
      601 tttttaaatg caagcttgcg acgtggacga tccattataa agtctgcgaa taccgcaatt
      661 gggataagat agtgcaacac cacattatcc caaggcacag ccgtcagagc gacatcgtcc
      721 atatgtgaca gcaggacaga gaagccaata ccaaccacca agatatacac cgtagttgcg
      781 ctgcgcagta ggtcgaattt gctgccctta cccgcagcaa ttgctactgc actcagcaac
      841 agggcgatta ccgctaagat attcgtttca atagtgaagt agctaaagaa attctctggt
      901 ctgaacgtcc cacgatctat cgtgactgcc atttctgtaa caatagacga aaacccaagc
      961 agtgcaaaaa acaacttgta cgaaataaga aatgtgttgc gattgacggt atttttagct
     1021 tgcttcatac caccattgta tcccatactg ctataggtaa aacattgcta acctgctaaa
     1081 atataccaaa agttcacttg aggaggcgcc gtgtcaaaac atcgtcgaaa tatcttcgga
     1141 gcatcaggcg gcatgtttat cggcatgatc attactggca ccgctctcgg acttttactt
     1201 aacaatgtac ttgttggtag catgctcggc gtcagcgtcg cgctcattat taccacgcta
     1261 ctaagaaaag acgaataaat cttggggcca attcgatatt gcaacgatcc acggcagact
     1321 ttcttgtatt acagtcagaa taaaacttac tctacgacgg gagcctgcga cgggtcggac
     1381 tttagtctag ctggattacg aatcccaaca agcgatacaa atccacccac aacgaacagc
     1441 acgatggcaa gaacaagtcc attacgaaaa cctgcaagat tgagattgta tccagtcacc
     1501 gtgccaataa gcgctaccgc taccagccca gctacgcgcg caacaacgtt gttaatcgcc
     1561 gaggcaatac cagatcgttt actatcaacc gcgcccaaga tagctgcggt aagcggcgca
     1621 acggtaaccg atagtccaat accaaccagc aaaatacctg gcaacatcgc ccagtagttc
     1681 gccgtcgcgt caataaatag catcgtcaca aatccagcag ccacaatcag tggcccaaca
     1741 gtcataaata tccgggggcc gtattcgcca gacagtttcc caaagcgcga cgcaaggaag
     1801 aacatcaaaa tcgacatagg caccatcgta aggccggcag ccgtagcggt gtaatggccg
     1861 acttgctgca caaaaatcgt aatcaagaac gcaacaagcg aaagtccagc atatatactc
     1921 agtgtagcga tattaccagc actgaagtta cgcgaacgaa atagccccag tggcatcatt
     1981 ggttgcgcgg ccaccttttc acgccatata aacaaaataa atgaaacaac cccgaccgcc
     2041 attgtgccgt aaataagcgg ataagcccaa ccaaaatggg gctgctcaat caatgcgtat
     2101 acaatgccgc caagactcag tgcgccaagt accgcgccgt accagtcaag tctaacgcgc
     2161 gtcgttgtat catgctcatc tttcagcctt gcaagcaaat ataccgtcac cgcgatcggc
     2221 aatacattca atagaaagat atagcgccac gagccaaggt caataagtac gccaccaaca
     2281 aatggcccga tcagcatcgc aatacccgtc cacgacgtcc acgcgccaat agccgcatgt
     2341 tgtctatcct tcggtacgcg cgacataatc agcgcaagcg agctcggcac aagaagcgca
     2401 ccagtagtgc cctgtaacaa acgcccaata atcaacaatt cttttgttgt tgccgcagca
     2461 caaagcaggg aagttacacc aaatccaatc agtccaatca tcattactcg tttgcgccca
     2521 ataatatccg atagcgaccc agctacaagc ataaatgcac caagtgtaat taaatacgca
     2581 tcaacaaccc attgctgcgt cgacaaaccg ccacctaaag tgtgttggat cgctggcaat
     2641 gccacattca caattgagcc atccaaaaag gcaataatag aggcaaggat ggcaatagcc
     2701 aaaacgagac ggtaggtaac ttctttcatg tccttagtat agcctgcgta gacagggtat
     2761 acctaatcat tttcagagac ggtcgcagga gccaattcgc gttgcgcctg ttt
//
LOCUS       DATIIX010000105         2694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_52601, whole genome shotgun sequence.
ACCESSION   DATIIX010000105 DATIIX010000000
VERSION     DATIIX010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52601"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /gene="dnaB"
                     /locus_tag="VLF64_02955"
     CDS             complement(<1..1047)
                     /gene="dnaB"
                     /locus_tag="VLF64_02955"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HSW90932.1"
                     /translation="MAAKKEVVAGKIPPQNLDAEMSLLGAILIDDEVIADASEIVDVK
                     DFYDKRHALIYGGMVRLYERHRPIDLLTLTDELKKKDQLETVGGSAYLTELTNYVPTA
                     AHAASYAEIINQKAIRRRLIKASAEISEMGFDEETSTQELLEKAEAELFSVSDQSIKQ
                     DLVSIESILTESFDRIEELHRNKGQLRGIRTGYRDLDNMTAGLQRSDLIILAARPAMG
                     KTTLVTNLAYNVATIAKLPVLYFNLEMSKEQLIDRMLADASGVDSWNIRTGNLSDEDF
                     SKISEAMGEMAEAPIFIDDTPGLSVLEMRTKARRAAHDQPLGLIIVDYLQLMEASGNH
                     NGNRVQEVSEISRGL"
     gene            complement(1047..2459)
                     /gene="dnaX"
                     /locus_tag="VLF64_02960"
     CDS             complement(1047..2459)
                     /gene="dnaX"
                     /locus_tag="VLF64_02960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HSW90933.1"
                     /translation="MGQALYRKYRSKSLDEVVGQSHITDMLRRAIASGNVTHAYLLTG
                     PRGVGKTSIARILAHEINQLPYTDESTHLDIIEIDAASNNSVEDIRNLRDKVAIAPTS
                     APKKIYIIDEVHMLSKSAFNALLKTLEEPPEHVVFILATTDVEKLPATIISRVQRFNF
                     RLIDSDDAVKHLRAISDAEHIDINDEALQLVARHGQGSFRDSISLLDQLRNLSDSTIT
                     PDLIESVVGLASNEMVLALLNAYDSQNLAKVIELINNAEKRGISAITITDQLVHAVRA
                     DITTHPERLPLLDKLLEVAKSTWPFVKLITALASNTIRTAAQATMPAPTAPSKTLSNP
                     TITSKSIVNPTAKPKATDVPELKAPEKFVEPPKPLGPEQPFPWEEFITNIKTSSPGAH
                     SILGKCDYAFDGTTLTIFTGKPFAKKQLDKSLPIMQAVLESVGVQDGVISVLGSTKPS
                     DKSAITAALDIMGGGEEVEL"
BASE COUNT          600 a          595 c          683 g          816 t
ORIGIN      
        1 gagcccgcga gaaatctcac tcacctcttg tacgcggttg ccattgtgat tgcctgacgc
       61 ctccataagt tgtaagtagt caacgatgat aagaccgagt ggttggtcgt gggcggcgcg
      121 gcgggctttt gtgcgcattt caagcacgct caagcctggg gtgtcgtcga tgaagattgg
      181 cgcttcggcc atctcgccca ttgcttcgct aattttactg aaatcttcgt cgcttaaatt
      241 cccggtacga atattccagc tatcaacacc cgatgcgtcg gcaagcatac ggtcgatcag
      301 ctgctcttta ctcatctcaa ggttgaaata cagtacgggc aactttgcaa tcgttgctac
      361 gttgtaggca agatttgtca cgagagttgt cttacccatt gctgggcgcg ccgcgaggat
      421 gatgagatca gagcgttgca gtccggcggt catattatca agatctcggt agccggtgcg
      481 tatgccgcgg agctgacctt tatttcgatg aagctcttcg atacgatcaa agctttcagt
      541 cagaatactc tcgatactga caaggtcttg cttaattgat tggtcgctta cgctaaatag
      601 ttcagcctcg gccttttcga gtaattcttg tgtgctggtc tcttcgtcaa atcccatctc
      661 actaatttcg gcgcttgctt taataagccg gcgacgaatg gccttttgat taatgatttc
      721 agcatagctt gcggcatgcg ccgcggtcgg tacgtaatta gtgagttccg taagatatgc
      781 tgaaccgcca actgtttcca gctgatcttt tttcttgagt tcatcagtga gtgttaaaag
      841 atcgatcggc ctatgacgtt catacagccg taccatacca ccgtaaatca acgcatggcg
      901 cttatcgtaa aaatccttta cgtcaacgat ttcacttgca tcagcaataa cttcgtcgtc
      961 aattaaaatt gcaccaagta agctcatctc agcatcaaga ttttggggcg gaattttccc
     1021 tgcgactact tctttttttg cggccattat agttctacct cttctcctcc acccataata
     1081 tcgagtgctg cggtgattgc acttttatct gatggttttg tactccctag cacactaatg
     1141 acaccgtctt gcacgccaac actttcaagt acggcttgca tgattggcag tgacttatcg
     1201 agttgttttt tggcgaacgg tttgcccgta aaaatcgtca atgttgtacc atcaaatgca
     1261 taatcacact taccgagaat actgtgtgcg ccgggtgacg aggtctttat attggtaatg
     1321 aattcctccc atgggaatgg ttgttctggt ccgagtggtt ttggtggttc aacgaatttt
     1381 tctggtgctt ttaattctgg cacatcagtc gcctttggct tagctgtggg atttactatt
     1441 gattttgaag ttatagttgg gtttgataaa gtttttgaag gcgcggttgg tgctggcatg
     1501 gttgcttggg ctgcagtgcg gatcgtattt gatgccagtg cggtgatcag tttgacaaat
     1561 ggccatgtgc tttttgcaac ctcgagtaat ttgtcgagta gcgggaggcg ctcgggatgt
     1621 gtggtgatat cggcgcgaac agcgtgcaca agttggtcgg tgatcgtaat tgcagaaatg
     1681 ccgcgctttt cagcattatt aataagctcg atgactttgg cgagattctg agaatcgtag
     1741 gcgtttagca gtgcaagtac catttcgtta cttgcaaggc cgacgacgct ttcgataaga
     1801 tctggtgtaa ttgtcgagtc gctcaaattg cgcagttgat cgagcaggct gatactatcg
     1861 cggaagctac cttggccgtg ccttgcaaca agttgcagcg cttcgtcgtt aatatcaata
     1921 tgttcggcgt cacttatggc gcgtaagtgc ttgacagcat catcactgtc gatgagccgg
     1981 aaattaaagc gctgtacgcg gctgatgatg gttgcgggta gtttttcaac atctgttgtc
     2041 gcgaggataa atacgacgtg ctctggtggt tcctcgagcg ttttaaggag agcgttaaat
     2101 gccgatttac tcagcatgtg cacctcgtcg atgatataaa tcttttttgg cgcgctggtg
     2161 ggcgcgatgg caactttatc gcgtaaattg cggatgtctt cgacggaatt gttacttgcg
     2221 gcatcaattt cgatgatgtc gagatgggtt gattcgtcag tgtatggcaa ttgattgatt
     2281 tcgtgcgcca aaatgcgggc gattgatgtt ttgccaacgc cacgcggccc tgttagcaaa
     2341 taggcatgcg taacattgcc gctcgcgatt gctcggcgca gcatatcagt aatatgcgat
     2401 tgaccaacaa cctcatctaa agatttcgaa cgatattttc ggtatagtgc ctgccccatt
     2461 gcttttatta tacctcgaga gctacagatt atgtgttgtg attttattag gccgctacag
     2521 cgcctggcat ggaggcgttt tcgagatagc atacgtatgc gccgcgtttt attagtatgt
     2581 tcgatggaaa cgaaaacgac catcgaactg gttggttata gccgatttgg cgaacttttt
     2641 cagtggtcat ggccattagc cacctaagcg tttattgcag taggaagtgc catc
//
LOCUS       DATIIX010000106         2117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_414083, whole genome shotgun sequence.
ACCESSION   DATIIX010000106 DATIIX010000000
VERSION     DATIIX010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414083"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="VLF64_02965"
     CDS             complement(<1..1246)
                     /locus_tag="VLF64_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HSW90934.1"
                     /translation="MDEDEVQQRRREQDEHSTKQRGALLGLDYLDTREFESELPLAQD
                     VMAIEKMHQDKMVPLVKGSGDAVYQFGVTTQTPQSVLEKMRREYADRGETVHFSLISM
                     SGYRALMLRYDPPKKVIYNDIEIAKEGDSDTITEVSKTLNSVSSEELFDYLIRQADKL
                     GASDIHIENERDSIRIRMRVDGALHPVALIERERYRVIMGELASRAGVSTASNESQSG
                     HMQKEITTERGTHLLNLRVETVPTMYGQDAVLRLFNFDESLLNLDLLGIPEKQRREID
                     EVVSHPRGLVLMVGPTGSGKSTTLYSMLNALNTPDRKLITLEDPIEYGLTGISQIPID
                     TTHGQSFADALRSVLRLDPDVVMVGEIRDTDTARTAIQASITGHLVLSSFHANSTSTA
                     FSRMVDMIGVNPIFSSAIRLIIA"
     gene            1290..1793
                     /locus_tag="VLF64_02970"
     CDS             1290..1793
                     /locus_tag="VLF64_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HSW90935.1"
                     /translation="MDKMKPEITLVLHNIRSIYNVGSILRSAEGFGVTSIVCCGYTPY
                     PIIAGDTRLPHIRDKITTSIHKTALGAEKMLQISHFETLDEWFTQNSLPVVALEQTSG
                     SVTPRQFVAPTAFALLLGEEVYGIEPQYLERCEAAIEIPMRGKKESFNVSVATGVALY
                     AILFGDD"
     gene            complement(1806..>2117)
                     /locus_tag="VLF64_02975"
     CDS             complement(1806..>2117)
                     /locus_tag="VLF64_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90936.1"
                     /translation="IAKPTASASKPAAAAPTRPIPSVDVPIEALKTIYVRIQNPNDHE
                     QLLSLKRICSLYPGQNTIVMMLGDNKSNAIRLPFKVDVQVADLTGQLTQLLGDESVVI
                     K"
BASE COUNT          525 a          488 c          529 g          575 t
ORIGIN      
        1 gtgcaataat taggcgaatc gctgagctaa agattggatt aaccccaatc atgtcaacca
       61 tacggctgaa ggcggtgctt gtagaattag catgaaagct tgaaagcacc aggtggccag
      121 taatcgaggc ctgaattgcc gtacgggcgg tatctgtgtc acgaatctcg cctaccatca
      181 ccacgtcagg gtcgaggcgc agcacactgc gaagtgcatc agcaaaggac tggccgtgtg
      241 tggtatcgat tggaatctga gatatacctg tcaagccata ctcgattgga tcttcaaggg
      301 tgataagctt gcgatcaggt gtgttaagcg cattaagcat actatagagc gttgttgatt
      361 taccacttcc agtaggaccg accattagca caaggccgcg cgggtggctg acgacttcgt
      421 caatttcgcg acgttgcttt tcaggaatac ctagaagatc aagatttagt agcgattcgt
      481 cgaagttaaa gaggcgaagc acggcatcct gaccatacat tgtcgggaca gtttcgacgc
      541 gaagatttag caagtgtgtt ccccgctcgg ttgtgatttc tttttgcatg tgaccggact
      601 gtgattcatt tgaggcggta gaaaccccgg cacggcttgc caattcaccc atgatcacac
      661 gatatcgctc gcgttcgata agtgccacgg ggtgcaaggc gccatcgaca cgcatgcgta
      721 tgcgaatgct gtcgcgctcg ttctcgatat gaatatcgct tgcaccgagc ttatcggctt
      781 ggcgaatcaa atagtcaaag agctcctcgg acgagacgct attgagtgtt ttacttactt
      841 ctgtaattgt gtcgctatca ccctctttag cgatttcgat gtcattgtag atcaccttct
      901 tgggtggatc gtaccgcagc atgagcgctc ggtagccact catgctaatt aatgaaaaat
      961 gtacagtctc gccacgatct gcgtattcgc ggcgcatctt ttcgagtact gattgtggcg
     1021 tttgcgtggt aacaccgaac tggtagaccg catcaccact gcccttaaca agtggcacca
     1081 ttttatcttg atgcattttt tcgattgcca ttacatcttg agcaagtggc aattctgact
     1141 caaattcacg tgtatcgaga tagtccagcc ccagcagggc accacgctgt ttcgtcgagt
     1201 gctcgtcttg ttcgcgacgg cgttgctgaa cctcatcttc atccatagac ttagtgtaac
     1261 aaatcgccta tggtttttat agtggtacaa tggataaaat gaagcctgaa atcactcttg
     1321 ttctccataa tatccgtagt atttacaatg ttgggagtat tctgcgtagc gccgaagggt
     1381 tcggcgtgac ttctatcgtg tgctgtggct atactccgta tcctataatc gctggcgaca
     1441 cacgcctacc gcatattcgc gataaaatta cgactagtat tcataagact gctcttgggg
     1501 ccgaaaaaat gctgcaaatt agccattttg aaacgcttga tgagtggttt acgcaaaatt
     1561 ccttgccagt agttgcgctt gagcagacat caggttcggt gacaccacga caatttgttg
     1621 cacctacggc attcgctttg ctcctcggcg aagaggtgta cggcatcgag ccacaatacc
     1681 ttgaacgatg tgaagcggca atcgaaatac cgatgcgtgg caaaaaagaa tcatttaatg
     1741 tgagcgttgc aactggtgtc gcactctacg caattctgtt tggtgacgac taaacgagta
     1801 ggactttatt taatgacgac agactcgtca ccaagtagct gggtcaattg accagtgaga
     1861 tcagccactt gtacgtctac tttgaatggc aagcggattg cattcgactt attgtcgccg
     1921 agcatcatca caatcgtatt ttggccaggg tacaaactac atatcctctt gagcgaaagt
     1981 agctgctcgt gatcattcgg attttggata cgaacatata ttgtcttaag tgcttctatg
     2041 ggtacatcga ccgaaggtat ggggcgagtt ggtgcagcag cagcgggctt agaggctgat
     2101 gcagtaggtt ttgcaat
//
LOCUS       DATIIX010000107         1936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_552685, whole genome shotgun sequence.
ACCESSION   DATIIX010000107 DATIIX010000000
VERSION     DATIIX010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552685"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            529..1059
                     /locus_tag="VLF64_02980"
     CDS             529..1059
                     /locus_tag="VLF64_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90937.1"
                     /translation="MPNDQNITKSISLQNDGGQLVLKVDYSHHQSETGDVVAIGRIMD
                     AFERQANSMLASSEDLLSDAKLPRGRRHIASMHSIKVDGRTLRIRADIKTTRKVDDFT
                     LICFADGLIGRTIPCIFAALKPEETAAFVLARLSRLLLRTMRQTAMDMYRLMPPSILF
                     GPFEATTGATVAVQEP"
     gene            complement(1463..>1936)
                     /locus_tag="VLF64_02985"
     CDS             complement(1463..>1936)
                     /locus_tag="VLF64_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSW90938.1"
                     /translation="LQYQWHGKNIVEVLDLTIADAVSFFEYPAVTKQLQALFDVGLDY
                     LTLGQPLSTLSGGECQRVKLASELHKSGSMYVLDEPTTGLHMSDIARLLTILNRLVDS
                     GNTVIAIEHNLDVIRQADWIIDIGPEAGQAGGRIVFAGKPKDLHTAKESITRQFI"
BASE COUNT          475 a          543 c          478 g          440 t
ORIGIN      
        1 gtaggcgccg cccttgcacc actcctcgct gctattggcg caattgcagc gctcgtcacc
       61 gaatgtacaa tcgtcgtgga acgcgaagag aaatagtctc aataccgggc gcaaaaatcg
      121 acgaggatac cgataagtgt agggtgaata gaaaaatagg ctaactcatc aattcaggaa
      181 cgcttcaatc acttcttgtg gcagttgccc tatatatttg ggatcgtacg aaagttttgc
      241 gagtggtatt ttaccgacaa ataagcgcat aaactcatac ttgtctaagt tacgcagcag
      301 ctctattaca tcactattga ccttacttaa cttatattgt aatataaaca tgttattcat
      361 cctagcggct ggatacgacc ctcacaaaca aactacaaat tcttctacgc agatgaggga
      421 aggtgttata acaaatagtt atagctcatt ccctcatctg cgcgaaccgt tcgcgcatat
      481 aaccctacat tctgtggggt tggtgagtac cggagaagga atacgtatat gcccaacgac
      541 cagaacatca cgaagtccat ttcgctgcag aacgacggcg gccagctcgt gctgaaggtg
      601 gactactcgc accaccagag cgagaccggc gacgtggtgg ccatcggccg gatcatggac
      661 gccttcgagc gccaggcgaa cagcatgctc gcctcctcgg aagacctcct ctcggacgcg
      721 aagctcccga gaggccggcg gcacatcgcc tcgatgcaca gcatcaaggt ggacggacgg
      781 acgctgcgga tcagggcgga catcaagacc acccgcaagg tggacgactt cacgctcatc
      841 tgcttcgcgg acgggctcat cgggagaacg atcccctgca tcttcgcggc actcaagccc
      901 gaagagacgg cggcgttcgt cctggcccgc ctctcccggc tcctcctgcg gaccatgcgc
      961 cagacggcca tggacatgta caggctgatg ccgccgtcga tactgttcgg tcccttcgag
     1021 gcgacgaccg gtgccacggt ggccgtccag gagccgtaga gagtctgcca cgatcaacgc
     1081 gggcgcggcg gcgatgccgc gcccgcacgg tctggtgtat ggaagtagtt tgtttgtgag
     1141 actacttcga acttatttac tctatcaaaa aagacacctc catagttggg gtgtcttgaa
     1201 ttttagttac taaaccaagg cgtttcattc aaccaaccga tcagttgtct tttcagtgag
     1261 ctgacgatct ccaatatcac gggacgagcc gcaggcttgt cgaaccacgc aacctttcca
     1321 gattgttttt gtactgttct ttatagaggg tgacgcgttc ttcgaatgcc tcgacccgct
     1381 caacctcttc tggaggcggt ggtggcatgg tgagcgccca tcctgctcgt tcacttgtgt
     1441 gggcagtggg cgggccatac ccctaaataa attgtcgcgt aattgactct ttcgctgtgt
     1501 gcaaatcttt tggcttacca gcaaacacaa tacgcccacc agcttgccca gcctctgggc
     1561 caatatcgat aatccaatcc gcctggcgaa tcacatccaa gttatgctcg atcgcaatga
     1621 ccgtattacc actatcaacc agccgattaa gaatcgtaag caagcgagca atgtccgaca
     1681 tatgcaaacc tgtcgttggc tcatcgagca catacatact gccagattta tgcagttcac
     1741 tcgcaagctt cacacgctgg cattcaccgc cagatagcgt gctcagtggc tgcccaaggg
     1801 taaggtaatc gagcccaaca tcaaacaacg cctgcagttg ctttgtgacg gcaggatatt
     1861 caaagaagct caccgcatcc gcaatcgtca gatcaagcac ctcaacgata tttttgccat
     1921 gccattggta ctgcaa
//
LOCUS       DATIIX010000108         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_136791, whole genome shotgun sequence.
ACCESSION   DATIIX010000108 DATIIX010000000
VERSION     DATIIX010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136791"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VLF64_02990"
     CDS             complement(<1..308)
                     /locus_tag="VLF64_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90939.1"
                     /translation="MIEVPQEFSKPAGFPVEIPDKCRRCVTLARFAVKYADLRAKIET
                     AEEVGISGALPSQWADQVAKVGKMSADEAVAFVASHEPELRAEFVGRLNSMDRACGDQ
                     "
     gene            614..853
                     /locus_tag="VLF64_02995"
     CDS             614..853
                     /locus_tag="VLF64_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90940.1"
                     /translation="MNNLINSPVSITALGFGRDMQPIPKRMEWGGRTYQFIDRGIRVI
                     SRRGEQIHSTVTMSDGERSFCLRETAGAWMLLSVC"
     gene            complement(917..1663)
                     /locus_tag="VLF64_03000"
     CDS             complement(917..1663)
                     /locus_tag="VLF64_03000"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HSW90941.1"
                     /translation="MRIARSVVALTIILAATLSYGHSASADSITYTNTATQSDASAKT
                     EPAKIVSTPAVTVQIGDTLTAIADAHSTTVNDIMAANAINDPNLIEPGQVLQIPAQPQ
                     GVTGFSHSLKAAAAAFMVPPVQLAQPQVQAAATNVPAQPTHHVAGVAGNTYVVGTCTW
                     YVKNRIPSLPNQLGNGGYGWLATAVAFGYVTGSSPAPGAIGVESGHVVYVESVNSDGT
                     VNISEMNYAGQIGVVHYRTTSVGEFQYIYV"
     gene            complement(1881..>2063)
                     /locus_tag="VLF64_03005"
     CDS             complement(1881..>2063)
                     /locus_tag="VLF64_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90942.1"
                     /translation="ERDGDRHDGRNHTTPVAEADGQDGVHNHGDNEQWKDDIHHGLLK
                     FEMADGVNLNKYYFTI"
BASE COUNT          523 a          530 c          455 g          555 t
ORIGIN      
        1 atttgatccc cacaggccct gtccatactg ttaagtcgcc ccacaaactc agcccttagc
       61 tctggttcat gacttgcaac aaacgccacc gcctcatcag cgctcatttt acctaccttt
      121 gcaacctggt cagcccattg acttggtagc gctccggata ttccaacctc ctcagcggtt
      181 tcaatcttcg cacgcaaatc ggcgtactta accgcaaagc gagcaagggt gacgcatcgt
      241 cgacacttat ccggaatttc tacaggaaac cccgctggtt tagagaactc ttgaggcacc
      301 tctatcatag tattagcata caatattcct gaatttttgc atctttttca cacttttatt
      361 taacaattgc cagtcttgac gcgtacggcc taagcaccca caatagaaga tggttatggt
      421 agtacttttg ccatttatgg cagatttcca ctcccaaacc gtacattagc atgaggctct
      481 cctagttaag gggaaccttc cgaaatcacg acttcaggca tacgctgtac tcgctaatta
      541 agtggggcag gccagcggcc ttgtcgttca tactccacca cttataaacg gtaatcacat
      601 aggagagtat gctatgaaca atttaataaa tagtcctgtt tctattaccg cacttggctt
      661 tggtcgcgac atgcaaccaa tcccaaagcg catggaatgg ggaggccgca cttatcaatt
      721 catcgaccgt ggtattcgcg tcatctcacg ccgaggtgag caaatacatt caacagttac
      781 gatgagcgac ggcgaacgaa gcttttgtct tcgcgagacc gccggtgcat ggatgctact
      841 tagcgtctgt taagcctcta caggatgaaa aagcccccga ctaccaatcg gaggcgtttt
      901 tcgtttttag ggccatttac acgtagatgt attggaactc acccactgaa gttgtgcggt
      961 aatgcacaac accaatctgg cctgcataat tcatttctga aatatttaca gtaccatcac
     1021 tattgacaga ttcaacatag acaacgtggc cgctctcgac gccaatcgca ccaggagctg
     1081 ggcttgaccc cgtgacatac ccaaacgcga cagcggttgc aagccagcca tagcctccat
     1141 tgcccaattg attaggcagt gacgggatcc ggttctttac ataccatgta caagtgccga
     1201 caacatacgt attaccagcc acacctgcaa cgtgatgggt tggctgagcg ggtacattcg
     1261 tagcagcagc ctgaacctgc ggttgggcta gttgtactgg gggcaccata aacgcagccg
     1321 cagcggcttt cagcgaatgc gaaaaacccg taacaccttg tggttgtgct ggtatttgta
     1381 aaacttgccc cggttcgatc aaattcggat cattaattgc atttgccgcc ataatatcgt
     1441 tcaccgtcgt gctatgtgca tcggcaatcg cggtaagtgt gtcgcctatt tgcaccgtca
     1501 ctgctggtgt tgatactatc tttgctggct cagtttttgc cgaagcatca gactgagtag
     1561 cggtgtttgt gtacgtaatg ctatctgcag aggctgaatg cccatacgat agcgtggcag
     1621 caagaataat cgtcagcgct accaccgacc ttgctatacg cattattatc tcctaagatt
     1681 acacccccac ggataggcaa ctatagtcat tacacgtgga tttcgtgtac tgaaataccc
     1741 ttaaaaaaac gggattcaaa aaacacatag tcaataatat caaaccatag tgtttatgtc
     1801 aatctttagc acgctagtgg tagtggttta cagaggctgc gaggcatatt gtccattaag
     1861 gtatttgatt atattaatga ttatattgta aaatagtatt tgttgaggtt gacaccatcc
     1921 gccatctcga acttaaggag tccatggtgg atatcgtctt tccactgttc attgtcgcca
     1981 tggttgtgta cgccgtcctg gccatctgct tccgcaactg gcgtagtgtg attgcgaccg
     2041 tcatggcgat cgccgtcccg ctc
//
LOCUS       DATIIX010000109         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_552842, whole genome shotgun sequence.
ACCESSION   DATIIX010000109 DATIIX010000000
VERSION     DATIIX010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552842"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VLF64_03010"
     CDS             complement(<1..634)
                     /locus_tag="VLF64_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HSW90943.1"
                     /translation="MMTTYDERYKNLNDEQRQAVDLVDGPLLVVAGPGTGKTELLSMR
                     VANILKKTDTLPENILCLTFTESGATAMRSRLSDIIGPQAYKVAIQTFHSFGTEVINQ
                     NREYFYRGAEFQPADDIASYDILTGIFDELDYSNPLASKNNGEYVHLSDTLRAIGELK
                     QAGLSSDELIAILNANDRTLDAIEPDLADIFSNRPSVSMLSMLVPIAEKVA"
     gene            687..1178
                     /locus_tag="VLF64_03015"
     CDS             687..1178
                     /locus_tag="VLF64_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90944.1"
                     /translation="MDNQALLDDLSLEKSAKERFGVALDVDSVIVRHIDVGQSAYATL
                     FLSSKKQLYCYIDGPTKLLLSDIKKIVSRMGLRPELFFPPKGRHTYFDDIGRAKFREV
                     FPGRRDVHDNDIIFYRTLAPYSPALVLVSEVKGGVVYQVDHDARGGWRPAVKFAYRRI
                     KTS"
     gene            complement(1175..1840)
                     /locus_tag="VLF64_03020"
     CDS             complement(1175..1840)
                     /locus_tag="VLF64_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90945.1"
                     /translation="MHKRLSRGFTIVEIATVIFIIALLAALIVVAFNNVQQQTRDTAR
                     ANDMSILGNLFERYYDKNGTYPVGCGGATCNSGYAWWGYTGTDIISTSTTLSQLTSML
                     SQTVSTIDPQLSTLQTPFIGVSYTIPSAPGYVYRGAQTITPPPNNTSGSIPIVKLSET
                     GSSRQCSLTVALNTTVGTDTAAYILAYYSESTKTWNVYLGKHGVRPTFDGTSTSSFCQ
                     LMN"
     gene            complement(1865..>2189)
                     /locus_tag="VLF64_03025"
     CDS             complement(1865..>2189)
                     /locus_tag="VLF64_03025"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90946.1"
                     /translation="EMREKITRLGKTAKAVLAPSEQALEYITGNEVVIVDPPRAGLHT
                     DVIARLLETKPKRIVYLSCNPVTQARDVAMLAEGYGVRAHRGYNFFPRTPHIEHLVVL
                     DHIEQ"
BASE COUNT          529 a          469 c          563 g          628 t
ORIGIN      
        1 atgcaacttt ttcagcaatt ggcacgagca tacttaacat actgacgctt gggcgattag
       61 aaaaaatatc ggcaagatct ggctcgattg cgtcgagtgt tctgtcgttt gcattgagta
      121 tggcaatcag ttcgtcactc gataaacccg cttgcttgag ttcgccgatt gctcttagcg
      181 tgtcacttaa gtgcacgtat tcaccattgt ttttgctggc gagcggattg ctgtaatcga
      241 gctcgtcaaa gatacctgtt aagatatcat aacttgcgat atcgtcagcg ggctgaaact
      301 cggcaccacg ataaaaatat tcacggtttt ggttaatcac ttctgtgcca aaactgtgaa
      361 acgtctggat tgctactttg taggcttgtg gcccaataat atcactgagg cgtgagcgca
      421 tggctgtggc gccgctttcg gtgaatgtaa gacaaaggat gttttctggc agggtatcag
      481 tttttttgag aatgttggca acgcgcatac tcagcagctc ggttttaccc gttcctggcc
      541 ctgcgacgac aagcaatggc ccgtctacga ggtctactgc ttgtcgttgt tcatcattga
      601 ggtttttgta gcgctcgtca taagtagtca tcatattcat tgtaccccag cacgctttgc
      661 aagaatatgg tataattacc gatattatgg ataaccaagc gcttctcgac gatttatcac
      721 tagaaaaaag cgcaaaggaa cgatttggtg ttgcgctcga tgttgattcg gtgatagtgc
      781 gacatataga tgttggccag tcggcgtatg cgaccctctt tctttcgagc aaaaaacagc
      841 tttactgcta tattgatggg ccaacgaaac tgcttttgag cgatatcaag aagatcgttt
      901 cgcgcatggg tcttaggcca gaattatttt tcccgccgaa ggggcgacat acatattttg
      961 acgatattgg tcgcgcaaaa tttcgtgagg tattccctgg ccgtcgtgat gtgcatgaca
     1021 atgatattat attttatcgc acacttgcgc catacagccc ggcactggta ttagtgagcg
     1081 aggtcaaagg cggcgtagtt taccaggttg accacgatgc tcgtggtggg tggcgtccag
     1141 cagttaagtt tgcgtatcgt cgtattaaga cgagctaatt cataagctgg caaaagctag
     1201 atgttgaagt gccgtcaaat gttgggcgaa ccccatgttt gcctaggtaa acgttccacg
     1261 tctttgtaga ttctgaatag tacgccaaaa tataggctgc ggtatcggtg ccgacggttg
     1321 tattaagcgc taccgtaagc gaacactggc gactgcttcc tgtttcagag agtttaacga
     1381 ttgggatgga cccgctggta ttgtttggcg gcggtgtaat agtctgcgcg ccgcgatata
     1441 catagcctgg tgcgctagga atggtatagc tcacaccgat aaaaggtgtt tgtagagtgc
     1501 tcaactgcgg atcgatagtg ctgaccgttt ggctgagcat agaggtaagt tgactgagag
     1561 tggtcgatgt actaatgata tctgtccccg tgtagcccca ccatgcgtag ccagagttgc
     1621 aggttgcgcc accgcagcct actggatatg tgccattttt atcgtagtat ctttcaaata
     1681 gattacctaa aattgacatg tcatttgccc tagctgtatc gcgcgtctgc tgttggacat
     1741 tattgaacgc gacaactatg agtgcagcta gtaaggcaat aatgaaaata accgttgcga
     1801 tttcaacgat ggtgaacccg cggctaagtc gcttatgcat atatttagtg taacagccta
     1861 acagttattg ctcaatatga tcgagcacta ctagatgctc aatgtgtggg gttcgtggga
     1921 agaaattgta accacgatgc gcgcgtacac catagccttc tgcaagcatt gcaacatccc
     1981 gagcttgtgt gacgggattg cagctaagat acacaatgcg ttttggcttt gtctcgagga
     2041 gacgcgcaat aacatcagta tggagaccgg cacgtggtgg gtcaacgatt actacttcat
     2101 tgccggtgat atattcaagt gcttgctcgc ttggtgcaag cacggctttt gccgttttgc
     2161 cgagtcgggt gattttttcg cgcatttcg
//
LOCUS       DATIIX010000110         1960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_332733, whole genome shotgun sequence.
ACCESSION   DATIIX010000110 DATIIX010000000
VERSION     DATIIX010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332733"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..824
                     /locus_tag="VLF64_03030"
     CDS             132..824
                     /locus_tag="VLF64_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90947.1"
                     /translation="MTLSSQDVQNVIGGVAVAVTFIVYSRRRIDARHDAARVILYEIE
                     QAESRLDDALVVIDNAENLVLHDRVQILPQNSWSDSRKQLINVLPSDVIKRINQFYND
                     CELLDGSLRQIDEMFLKNETEVRVNAFRVNADYFKARVDKFMYDKDDKSEKQDLERVN
                     KEMIIDANNRREYFNKEFPTLYSYAPVKPINDGRKIVARLRDYNLSQSRAGDYLRKNG
                     ASLFFRLINRSK"
     gene            1062..1841
                     /locus_tag="VLF64_03035"
     CDS             1062..1841
                     /locus_tag="VLF64_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90948.1"
                     /translation="MIDAMMCRQADRAGYTVDPSYSPGHVIRGIQIVNPDAERLSVVF
                     PPWHGGGVYGNVLASRIANHRDPDGSQSAVWTYDFADDIIHPSVDGVLAGYRTIRDRV
                     ARDLDSAVGPGRLYRSVDIYSVSLGTVAASVVASAYPNFRAATMVVPGSSLAHCLWEG
                     VRTQALRAALVANHAGLDVDALHDAWRELTTLHSITSFAGKAALVVRSWSDNIIPRDY
                     QTQVMAQLVAAGARVESPRATSFGHYATAANYCLRGTVGHA"
BASE COUNT          514 a          425 c          526 g          495 t
ORIGIN      
        1 gatcatcgat atacaattcg ccgttaataa gatttgtttg gcacgattca taagtgtgga
       61 taaagtttgc acgaagtcgt aaagtaatat caagctacta gggctatgtg ggaaaataat
      121 cggagataat tatgaccctt agctcacaag atgtacaaaa cgtaataggt ggtgttgctg
      181 ttgccgttac attcattgtc tattccaggc gacgaattga cgcaaggcac gatgctgcaa
      241 gagtcattct ttacgagatt gagcaagctg agagcagact ggatgatgcc ttggttgtaa
      301 tagataatgc ggagaaccta gtactgcatg acagggttca gatcttacct cagaatagct
      361 ggtcagattc aagaaaacag cttatcaacg tactgcctag cgatgtcata aaaaggatca
      421 accaattcta caatgactgt gagttactcg atggctcgct taggcagata gacgagatgt
      481 tcctaaagaa tgagaccgaa gtacgagtga atgcctttag ggtaaatgct gattatttca
      541 aggctagagt cgataagttt atgtacgata aagatgataa atctgaaaag caggatttgg
      601 agagggtcaa taaagaaatg attatcgatg caaacaaccg acgagagtat ttcaacaaag
      661 aattccctac actgtatagc tacgcgccag ttaagcccat caatgacggc aggaagatag
      721 tagcaagact gagggactat aatctcagcc aaagcagggc gggtgattac ttacgcaaga
      781 atggcgctag cttattcttc agactaatta ataggtctaa gtaagagggc taaagcttca
      841 agggactgta tacgtctgtt gagcgttttt cacggtactt gaagtctaga tagtcgcccc
      901 aaagtaaatc acgccaacgg tgtaccttct caataatgag gggtgaacct ttttcgaggt
      961 gctccaccat aaaagcatta acagcacgtt ggtgctttca tattattgcg ggccgaatgg
     1021 gccattgatc tacctatgta ttgtgatata acaatacaat tatgattgat gccatgatgt
     1081 gccgacaggc agaccgagcg ggctataccg tggacccgtc ctactctcct ggccacgtta
     1141 tccgtgggat acagattgtt aacccagatg cagagcgctt gtcggttgtc ttcccgccat
     1201 ggcatggcgg cggggtatat ggcaatgtgc ttgcaagccg gattgcaaac caccgcgacc
     1261 ccgacggatc acaaagtgcc gtatggacgt acgattttgc cgacgacatc attcatccta
     1321 gtgtagatgg cgtactcgcc ggctatagaa ctattcgtga cagagttgcc cgtgacctcg
     1381 acagtgcagt ggggcccggc aggctctacc gctcggtaga tatttatagt gttagtttgg
     1441 gtacggtcgc cgccagcgtc gtagctagcg catatccaaa ctttagagcg gcgaccatgg
     1501 tggtgcccgg cagtagcttg gcccactgcc tgtgggaagg cgtgcgcacg caagctttgc
     1561 gtgctgcgct ggttgcaaac catgccgggc tagacgttga tgcgctccac gatgcgtggc
     1621 gagagttgac gactttgcac agtattactt cattcgctgg caaggcggcg cttgtggtcc
     1681 gttcgtggtc ggacaatata atcccgcggg attaccaaac acaggtgatg gctcagttgg
     1741 ttgcggcggg tgcacgggtt gaatctccaa gggcaacatc gtttggccac tacgctacgg
     1801 ccgcaaacta ttgcttgcgc ggcacagtcg gccacgcgta agcgttttgt ggtactataa
     1861 ccgtaataat ggttgcgatt actttgatgc tcattggcct ggtggccggc gcactcagcg
     1921 gtctagtagg tgtaggtggc ggtgtattaa ttgtgccagc
//
LOCUS       DATIIX010000111         3124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_442876, whole genome shotgun sequence.
ACCESSION   DATIIX010000111 DATIIX010000000
VERSION     DATIIX010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442876"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..925
                     /locus_tag="VLF64_03040"
     CDS             299..925
                     /locus_tag="VLF64_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90949.1"
                     /translation="MPPQQSQSGMTPVAPSGQYDFIVNGSNRPSESWLVHAPLKTRIL
                     VVGGGALVLLLVAWIVIALLSASGGTSARNLLAIAQEEAELVRISDGPAHNASLETTQ
                     NLASSTQLSVLSDEQSIITYLGTVNAAPTTTDLVKNRNTRTDSQLLAAKADGTYDQVY
                     ISIAQHQLTVYSQNLKRTFDATGNTTERQLLSAAYHHAQLLLTLSKQH"
     gene            complement(922..1245)
                     /locus_tag="VLF64_03045"
     CDS             complement(922..1245)
                     /locus_tag="VLF64_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HSW90950.1"
                     /translation="MDHKTVEEYLLSMPNARLDYPFGEGVAVYKVGDKMFALVEEKSD
                     PLRISLKCDPQLSEILREKYESVMPGYHLNKKHWNTIVLSGQLPLDELQGLIRHSYQL
                     IEPGN"
     gene            complement(1261..1749)
                     /locus_tag="VLF64_03050"
     CDS             complement(1261..1749)
                     /locus_tag="VLF64_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90951.1"
                     /translation="MAYVPYVGYALVWARSWVGIAALVYLPTLLILVEEVNRLSSYYR
                     LALPYRLASYHRRAKQETHKSMLTVPMTGVFVVAIVVLAWQPIAHALFHSNTVAIIHN
                     RLSISLPLCQNADNTNNISIMNTSTQTAASGSASGGSANSGNASNSNNTNINITITGS
                     HC"
     gene            complement(2131..2709)
                     /locus_tag="VLF64_03055"
     CDS             complement(2131..2709)
                     /locus_tag="VLF64_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90952.1"
                     /translation="MFAFHKINPMVRAIGTMVAVVGVASGVTFASLTTNTVALTANEL
                     DVTSDVLRISNGGAFSSTGVTGFTNSNLIVNTEGPKNAFYFQNLANAALTLSATVPGS
                     PTVNHIATNGIHVKFYDKDGTTLLLSTTLQALEAGPVALGDQLDALAQGNGGVAGTEG
                     NYFYSVSVDASAVTGSPASVPSFEIDFSGTTV"
     gene            complement(2821..>3124)
                     /locus_tag="VLF64_03060"
     CDS             complement(2821..>3124)
                     /locus_tag="VLF64_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HSW90953.1"
                     /translation="GKPESIVVDELTDLIHDQFASEPAVLAGNSIHQDRRFIRQWWPE
                     VEALLHYRMFDVSSFKVWMQGKYGLEFEKKETHRALDDIDESIAELQYYLEWFRKN"
BASE COUNT          775 a          829 c          724 g          796 t
ORIGIN      
        1 ttatggagaa taggccgcta aattcaggtt ctaatgatcg ctcatgtaga tatctcgcat
       61 atctcgtcgg taattccaac tccagcacaa agtcctgccc cgcaggttcc tcgcctaccg
      121 attcttgacc agcttcatgc cacataagta ctcccgttgc tacaaatgca gttaaacatc
      181 ttttgtagat atgcgatgta aaattactcg catacgtata cgccggcgga gcggtatact
      241 ataagttcgt gcaaaagcct tcttgagtgt ttcggtgggt tcaactacca taagaactat
      301 gcctccgcag caatcacaat ccggcatgac gcccgtggcg ccaagcggtc aatatgactt
      361 catcgtcaat ggcagcaatc gtccctcaga aagctggctt gtccacgcgc cgctgaaaac
      421 gcgtattctg gtagtcggcg ggggcgcact ggtactgcta ttggtcgcct ggatcgttat
      481 cgctttgctc tccgccagtg gtggcacatc ggcccggaac ctgttagcca tcgcccagga
      541 ggaggccgaa ttagtccgta tttcagatgg gccagcccac aatgccagcc tcgagaccac
      601 gcagaattta gccagcagta cgcaactgag tgtccttagc gacgagcaga gtattatcac
      661 ctacctaggg accgtcaatg ccgctcccac aactacggat ctagtcaaaa atcggaatac
      721 ccggaccgat tcgcagctcc tagctgccaa agccgacggc acgtatgatc aggtgtatat
      781 ctccattgcc cagcatcagc tcaccgttta cagccagaat ctgaagcgta ctttcgacgc
      841 gaccggcaat actaccgagc gccagctact tagcgccgct tatcatcacg cgcagctgct
      901 actgacactc agcaaacagc actagttacc tggctcgatc agttgatacg aatggcggat
      961 taatccttgg agttcgtcga gtggtaattg gccactcaaa acgatcgtgt tccaatgctt
     1021 tttattcaag tgatagcctg gcatgacgct ctcatacttt tcgcgcaaga tctccgacaa
     1081 ctgcgggtcg cacttcagac taatgcgcag cgggtcgctc ttctcctcga ccagcgcaaa
     1141 cattttgtcg ccgactttgt agacggcaac accttcgcca aacggataat ccagccgcgc
     1201 attcggcatg ctcagcagat attcctcgac ggtcttatga tccatgaccg ttagtataaa
     1261 ttaacagtgg ctgcccgtga tagtaatgtt tatatttgta ttgtttgaat tgctcgcatt
     1321 gccgctgttc gctgacccgc cgcttgccga acccgatgcc gccgtctgtg ttgatgtatt
     1381 cataatactt atattatttg tgttatccgc gttctggcac aaaggcagtg aaatacttag
     1441 ccgattgtga ataattgcaa cggtatttga atgaaatagg gcgtgagcaa tgggttgcca
     1501 ggctaatacc acgatggcaa ccacaaaaac ccctgtcatg ggtacagtca acatgctttt
     1561 atgtgtctct tgttttgctc tgcgatgata acttgccagg cgatacggca gcgccaagcg
     1621 ataatagcta ctcagccgat ttacttcttc gaccagaatt aacagcgtcg gcaggtatac
     1681 gagggctgct atgccgaccc aggatctggc ccatacaagt gcataaccaa catatggcac
     1741 ataagccatt actctacctt tgacactacc gccaactatg gggtatgtcg gctgcttcgt
     1801 tagcatcgcc tttggtaatg aaactaggga ttctgccggc tagtagaaat gttttgatga
     1861 tgcgatgcgt tacagtctgc cctggtatca acggattggc atacgtgatg acgtccccaa
     1921 tatgtaatgt cgagatggga acgcggtgca tgaatactaa tgagccccgc gcaatagctg
     1981 gacgcatgct gccagtcgga attatttcgg ctttccagcc cgtttccggc aaactaaata
     2041 gcagccatgc gccgcagccc agcccaagaa cgagcacacc taaactggtg aagactctac
     2101 gaatccaccg cattattcaa tttctctgta ttaaacagtc gtaccagaga agtcaatctc
     2161 gaagctcggc acactagccg ggctaccagt tacggcagac gcgtctacgc tgacactata
     2221 gaagtaatta ccttctgtgc ctgcaactcc accattaccc tgtgcgagcg cgtctagctg
     2281 atcacctaga gcaacgggac ccgcttcaag tgcttgaagc gttgtgctaa gcaatagtgt
     2341 ggttccgtcc ttgtcataga atttaacatg aatgccattc gtggcaatgt ggttgacggt
     2401 tggcgaacca gggacggttg cacttaaagt gagtgcagca ttcgcaaggt tttggaagta
     2461 gaaggcattt ttaggccctt cggtattaac aattagattg ctgttcgtaa atccagttac
     2521 acccgtacta ctaaacgcgc ctccattact aatacgtagt acatctgacg ttacgtcgag
     2581 ttcgtttgcg gttaaagcta cggtgtttgt tgttagacta gcgaacgtga ctcctgatgc
     2641 tacaccgacc acggcgacca ttgtcccgat agctcggacc atcggattaa ttttgtgaaa
     2701 tgcaaacatg cccacctcct cttattgtta ccattgggca gtgtgcggca catgcgcggc
     2761 cagtacagca tcatttctta cagagccgct gaatattaca tgaattatta ctgggattag
     2821 ctagttcttg cgaaaccact cgagataata ctgcagctcg gcaatactct cgtcaatatc
     2881 atcaagcgcc cgatgcgttt ctttcttctc gaattccagg ccgtattttc cttgcatcca
     2941 gactttgaac gagctgacat caaacatccg gtaatgcagc agcgcttcaa cttccggcca
     3001 ccattgccgg ataaaacgcc gatcctgatg gatagaattg ccggccaaaa ccgccggttc
     3061 cgaggcaaat tgatcgtgga tcagatctgt taattcatcg acaacgatcg actcgggctt
     3121 gcca
//
LOCUS       DATIIX010000112         6310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_442889, whole genome shotgun sequence.
ACCESSION   DATIIX010000112 DATIIX010000000
VERSION     DATIIX010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442889"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /gene="msrA"
                     /locus_tag="VLF64_03065"
     CDS             <1..306
                     /gene="msrA"
                     /locus_tag="VLF64_03065"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HSW90954.1"
                     /translation="FDPAKTSHGALLDYFFKIHDPTTKNRQGNDAGTSYRSVIFYKSD
                     DEKRAAEEAITRNQPHWEDPIVTQIEPLTKFWSAEGYHQDYLQKNPGGYTCHYERPW"
     gene            325..1881
                     /locus_tag="VLF64_03070"
     CDS             325..1881
                     /locus_tag="VLF64_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HSW90955.1"
                     /translation="MNTALRQKLTTLPNSPGVYFHKSADGEIIYVGKAAILKNRVRQY
                     FQSKKDFDVKTLALVNEIADTDWVETESEIDALFLESEMVKRYMPRYNILLRDDKSQT
                     FVRIDMRSEWPTVTFTRNPADDGAKYIGPFYNGFAVKKALRYLRKVFPYYTNEPRRDR
                     NSLDVQIGLEPRHDITSTEYKASLKKLIKYVEGGRLAIVKSLEQDMKRAAKNEEFELA
                     ASYRNKLSHLRELQRRIMFGDREFMDISKDKALTQLTELLGLASVPARIEGYDISHMS
                     GTNVVASMVVFTNGVSDRSNYRKFKMSRATNDDTANMAETISRRFSEKNIKAWGMPDL
                     ILIDGGKGQLHAAQAALELTGVQVPAVSIAKREEELVVAKNAPHIKDDFLQELIRSQQ
                     DGVSVIESGGYYFINLHVGRHNAGGHSRNLRGGDSLSPYDELTKLFQRIRDESHRFAV
                     SYHTTLKRQGAIKSQLDDIPGVGPVMKKKLLKQFGSVRGVSAAGETDLRAVVGPLLAS
                     RIRAHINRQE"
     gene            1907..3097
                     /locus_tag="VLF64_03075"
     CDS             1907..3097
                     /locus_tag="VLF64_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HSW90956.1"
                     /translation="MFHTNNRKRLFAKAKGELIILTAYDSVQQSGDMAAPFLQESSFW
                     WVTGINQPGWKVILDTARGEAMLVRPQRSDIDVIFNGESDDNEVRAISGIQHIISQRE
                     FEGQLRQLHRHHTIAYTLTQKEDHEFVLNPAKATLVAMLKRIFDSVQYSDSLLAELKA
                     IKQLDELGRMRAAMKLTCDTFSSVQSQLASFKTEAEIEAEFIYAFHKAGAHHAYEPIV
                     ASGSHACTLHYIENSGKLRAREMVLIDIGARVDGYSADVTRTYCRNPTKRQSEVHAVV
                     VEAEQAIIGLIMPGLPVADYLKQVDEIMKQALQKLGLLEDINDTETYRKYFPHAVSHG
                     LGVDTHDSLGRPRYLEAGMVLTVEPGIYIPEESIGVRIEDDILVTSTGCENMTGQLST
                     ALTI"
     gene            3134..3529
                     /locus_tag="VLF64_03080"
     CDS             3134..3529
                     /locus_tag="VLF64_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HSW90957.1"
                     /translation="MKRQLFTFVIRWVLNSFGLWVAVRIFGTGYSNEQISNSISVFLV
                     AGLIFSVVNAILKPIVIILSLPAILITLGLFTVIVNGLMVYISLKLAPGMHMTFLNSV
                     LTGLVLSLVNYIVSSALELEYGRTREEKL"
     gene            3526..3759
                     /gene="secG"
                     /locus_tag="VLF64_03085"
     CDS             3526..3759
                     /gene="secG"
                     /locus_tag="VLF64_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HSW90958.1"
                     /translation="MNLTTILQVVTVGSAVLLVIAILLQQRGASLGAGFGGSNELYTT
                     RRGLDKNLFEVTIVLAVIFVLSILISLVVPNLK"
     gene            3774..5573
                     /locus_tag="VLF64_03090"
     CDS             3774..5573
                     /locus_tag="VLF64_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HSW90959.1"
                     /translation="MDDEQKRWGKVRGGMKLNRKQLLRRMRRAEAVSTKHAHKFVIGR
                     INNIRLVSREITLWLVFVGVLIAGLGVQILWSQDSYMMTTPQGGGAYVEGAVGRIDTL
                     NPLFSSTDTEVSAARLVFSSLYNYDRTGTLHQDLAMDMVADSSQRVYTITMRGDAKWQ
                     DDKPVTAQDVVFTINLIKNPATRSPLRVNWLDISATALNNTSVQFTLPAVYAAFPQAL
                     TFPIMPMHLLKSLDPSAVRESVFSQSPVGSGPFKFSRLQQIDSATGQKVVHLVANTKY
                     YAGRPKLDRFELRSYPTETALIRAVNNGEVTGASDVSVTGVKEVKTKQATITSESVDS
                     GVYLLFNMQNPVLKDASVRQALQLATNTADIRKALGGGVLPLDGPLLQNQLTGVNVPH
                     APAPDVVKANQLLESDGWKLVNGYRVKDGQTLQLGITTTKDSEYEAVLKQVEAQWHKV
                     GVKVVTNVIDTSSTTSTFVQNVLQARNFDVLLYELSLGADPDVYAYWHSSQIGMSGYN
                     FSNYSNRIVDASLASARSRIEPDLRNAKYTLFVRQWFADAPAVALYQSAVEYVTASGV
                     QATRSGGRLVTLADRYADIQYWTVQNGLMYKTP"
     gene            5612..6064
                     /locus_tag="VLF64_03095"
     CDS             5612..6064
                     /locus_tag="VLF64_03095"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSW90960.1"
                     /translation="MNHEFDITVTKKINATPAEIWHALTDREAVKQWLFGTEVNSTWQ
                     QGASITYRGVWDGKPYEDKGVIQDITPEVLLKTTYFSPLSGKEDISENYNLVTYVIAR
                     NDDGTSGLSVTQENNDSQETADNMKGNWQQMLDGLAAYITSSKTMSKS"
     gene            complement(6106..6179)
                     /locus_tag="VLF64_03100"
     tRNA            complement(6106..6179)
                     /locus_tag="VLF64_03100"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6145..6147),aa:Cys,seq:gca)
BASE COUNT         1800 a         1400 c         1535 g         1575 t
ORIGIN      
        1 tttgacccag ccaaaacatc gcacggtgca ctgcttgatt actttttcaa gattcatgac
       61 ccgacaacca aaaatagaca aggtaatgat gccggtacga gctatcggtc agtaatattc
      121 tacaaaagcg atgatgaaaa gcgcgcagca gaagaggcga tcacgcgcaa ccaaccgcat
      181 tgggaagatc caatcgtcac gcagattgag ccgctcacga agttctggtc agctgaagga
      241 tatcatcaag actatctcca aaagaatcca ggcggctata catgccacta tgagcggcct
      301 tggtaattcg cgtatactgt aagagtgaac acagcactta gacaaaagct aactacactc
      361 ccaaatagcc ctggcgtgta cttccataaa tcggcggatg gcgagattat ttacgttggc
      421 aaggcggcga tactaaagaa tcgtgtgcgg cagtactttc agagcaaaaa agattttgac
      481 gttaagacct tggcactggt gaatgaaatt gcagataccg actgggttga gaccgagagt
      541 gagattgacg cactgttctt agagagtgag atggtcaagc gctacatgcc gcgctataac
      601 attttgctac gtgatgacaa atcccaaaca tttgtacgta ttgatatgag gagtgaatgg
      661 ccaacagtga cattcacacg caatccagcc gatgacggcg ccaagtatat aggcccgttc
      721 tataatgggt ttgccgtcaa aaaggcactg cgatatttgc gtaaagtatt cccgtattac
      781 acaaacgagc cgcgacgaga tcgcaatagc ttggatgtcc agattggttt agagccacgc
      841 cacgacatta caagtacgga atataaagca agtctcaaaa aacttattaa atatgtagaa
      901 ggcggccgtc ttgctatcgt gaagtcactc gaacaggaca tgaagcgcgc tgccaagaat
      961 gaagagtttg aacttgcggc cagctaccgc aataagctct cgcacctacg tgaattacag
     1021 cgtcgtatta tgtttggcga tagagaattc atggacatct ctaaagataa ggcgctcaca
     1081 cagctcactg aattgcttgg gcttgccagc gtgccagctc gcattgaagg ctacgatatc
     1141 tcacatatga gcggcacaaa tgttgttgca agcatggtag tgttcacaaa tggggtaagt
     1201 gaccgtagta actaccgtaa atttaaaatg agtagggcga caaacgatga taccgcaaat
     1261 atggctgaga caatctcgcg tcgttttagc gaaaaaaata tcaaagcctg ggggatgcca
     1321 gaccttatat tgatcgatgg cggcaaaggc cagctgcatg ccgcacaagc cgcacttgaa
     1381 cttaccggtg tacaagtacc tgcagtcagt atcgcgaagc gcgaagaaga actagtggtg
     1441 gcaaaaaacg caccgcatat taaagatgac tttttacaag aattaattcg ttcgcaacaa
     1501 gacggtgtga gtgtcattga atcaggcgga tattatttca ttaaccttca cgttggccgt
     1561 cacaatgcag gcggacattc gcgcaactta cgtggtggtg atagtcttag cccatatgac
     1621 gagctaacaa agctctttca gcgaatccgt gatgagtcac atcgctttgc agtgagctac
     1681 cacacaactc ttaagcgcca aggcgcaata aaaagtcagc ttgatgatat ccctggggtt
     1741 gggcccgtta tgaaaaagaa gctacttaag caatttggta gtgtgcgggg tgtttcggcg
     1801 gcaggcgaaa ctgatttgcg cgcagttgtc ggtccgcttt tagcaagccg cattcgggcg
     1861 catattaaca gacaagaata gtttgtagcg atgctacaat aaatatatgt ttcataccaa
     1921 taaccgcaag cgactcttcg ccaaagctaa aggcgaactt attattctca ccgcgtatga
     1981 cagtgtgcaa caatcgggcg atatggcagc gccattttta caggaatcat cattttggtg
     2041 ggtaacagga attaatcagc ctggctggaa ggtgattctc gatacggcac gaggtgaggc
     2101 tatgcttgtc cggccacagc gctcggatat tgacgttatt tttaatggtg aatcagatga
     2161 taacgaagtg cgtgcaatta gcggtattca gcacattatt tcgcagcgcg aattcgaggg
     2221 ccaattacgc caactgcatc gccatcatac aattgcgtac accctaacac agaaagaaga
     2281 ccatgagttt gtactgaatc cagcaaaggc tacgctggta gcgatgctaa agagaatctt
     2341 cgactctgta cagtattctg actcgctact tgctgaactt aaagcgatca agcagttaga
     2401 tgaacttggg cgtatgcgcg cagcaatgaa actaacttgt gatactttta gctcggtaca
     2461 atcccagctt gcttcattta aaacagaggc cgagattgaa gcggaattta tttacgcatt
     2521 tcataaagcc ggcgctcatc atgcgtacga gccaattgtt gcgagcgggt cgcatgcatg
     2581 tacgcttcat tacatagaaa actcaggcaa attacgagcc cgtgaaatgg ttctcattga
     2641 tatcggtgca cgtgtggatg gctattcggc agatgtgacg cgcacatatt gccgcaaccc
     2701 aactaaacgc caaagtgagg tgcacgcagt agtcgttgag gcagagcaag cgatcattgg
     2761 acttatcatg ccaggcttgc cggtcgcaga ctacctgaag caagtcgatg aaattatgaa
     2821 acaggcactt cagaaacttg gactgctgga ggatatcaat gataccgaga cttatcgtaa
     2881 atactttccc cacgctgtta gccatgggtt gggcgttgat acacatgaca gcttaggccg
     2941 accgcgttat cttgaggcgg gtatggtgct gacggtggag ccgggaattt atatccccga
     3001 agaatcaatt ggcgtacgga ttgaggacga tatcctggtt acctcaactg gctgcgagaa
     3061 catgacaggc caattatcca cagcgcttac catctagtat ttaactagca tattcgatat
     3121 aatatctaca acaatgaaac ggcagctttt tacatttgtc atacgctggg tactcaattc
     3181 ctttggttta tgggttgcag tacgtatatt cggcaccggc tatagcaatg agcagatctc
     3241 taacagcata tcagtcttct tggtcgcagg gctcatcttc tcggttgtta acgcaatcct
     3301 taagccgatc gtaattatct tgtcacttcc tgcgattctt atcactttgg gactctttac
     3361 ggtcatcgtg aatggtctca tggtatacat ctcgctcaag ctcgcaccag gaatgcacat
     3421 gacatttctt aactcagtac ttaccggatt ggtgctaagt ttggtaaact atattgtaag
     3481 tagtgcacta gaacttgaat acggacgcac acgggaggaa aaattatgaa tttgacaact
     3541 attttacagg tagtaactgt tggctcggcg gtgcttcttg ttatcgccat tctattacaa
     3601 caacgtggcg caagccttgg cgctggtttt ggtggctcaa acgaattata cacaactagg
     3661 cgtggcctcg ataagaacct ttttgaggta accatcgtac ttgcagtgat ctttgtcttg
     3721 tcaattctta ttagccttgt agtaccaaat cttaagtaac ggattacaat tacgtggacg
     3781 acgaacaaaa gagatggggt aaagttcgcg gtggtatgaa gcttaaccgt aagcagcttc
     3841 ttcgtcgtat gcgtcgtgca gaggctgtct caacgaagca cgcccacaag tttgtcattg
     3901 gccgcattaa taatatacgg ctcgtctcac gcgaaattac cctatggcta gtatttgtcg
     3961 gtgtcttaat tgctgggctg ggtgtccaaa ttctatggag ccaagacagt tatatgatga
     4021 caacaccgca aggcggcggc gcatatgttg aaggagcagt agggaggatt gatacgctta
     4081 atccgctatt ctcatctaca gatactgaag tctcagcggc acggttagta ttttcgtcac
     4141 tctataacta tgatcgaacg ggcacactcc atcaagactt ggccatggat atggttgctg
     4201 attcttcgca aagggtatat accatcacga tgcgtggtga cgcaaagtgg caagatgata
     4261 aaccggttac cgcacaagat gtcgtattta caattaacct cattaaaaat ccagccacac
     4321 gttcgccact gcgcgtaaac tggcttgata tatcagcaac agctctcaat aatacaagcg
     4381 tgcagtttac gctgcctgca gtttacgcag cgtttcctca ggcgcttacg ttcccaatta
     4441 tgccaatgca tcttttgaaa tcactcgatc catcggctgt gcgtgaaagc gtattcagcc
     4501 aaagtccagt cggaagcggt ccattcaagt tctcccggtt gcagcagatt gacagcgcaa
     4561 cgggccagaa agtggtacat ctggtcgcaa acacaaaata ctacgcaggt cgcccaaagc
     4621 tcgatcgatt tgagctccgt tcatatccta ccgagactgc actaattcgc gcagtgaata
     4681 atggcgaagt gaccggggcc tcagacgtat cggtgacggg cgtgaaagaa gtgaagacta
     4741 aacaagcaac tattacgtct gaatctgtcg atagtggagt ttatctatta ttcaatatgc
     4801 agaacccagt attaaaagac gcatccgttc ggcaggcgtt gcagctcgca acaaatacag
     4861 cagatatccg caaggccctt ggcggaggcg tacttccgct tgacgggcca ttgctacaga
     4921 atcaacttac tggggtgaat gtgccgcacg cgcctgcacc ggatgtcgtg aaagcaaatc
     4981 agttacttga aagcgatggt tggaaacttg taaatgggta tcgagtaaag gatgggcaga
     5041 cactgcagct cggaattaca acgacgaaag atagtgagta tgaggcagta ttaaaacaag
     5101 ttgaagcaca gtggcataag gtcggggtaa aggtggtaac gaatgtgata gacacttcaa
     5161 gtacgacatc aacattcgtg cagaatgttc tgcaggcgcg caacttcgac gtgctattat
     5221 atgaactttc actcggtgcc gatccagatg tctatgcgta ttggcactca tcgcaaattg
     5281 gcatgtcagg ttataatttc tctaattact caaatcgtat tgttgatgcc agccttgcaa
     5341 gtgctcgctc gcgcattgag ccggacttgc gtaacgcaaa gtatacatta ttcgtccggc
     5401 agtggtttgc cgatgctcct gcggtcgctc tttaccagtc tgcagttgag tatgtcacgg
     5461 ctagtggtgt gcaagcaaca agatcgggtg gtcgtcttgt gacgctcgct gatcgatatg
     5521 cagatataca atattggact gttcagaacg ggttgatgta caaaacgccg taaagcacgg
     5581 cgatgatata taaaagagaa ggagaatgag tatgaatcac gagtttgaca ttactgttac
     5641 aaaaaagatt aatgcaacgc cagctgaaat atggcacgct ctcacagatc gtgaggctgt
     5701 aaagcaatgg ctgtttggaa cagaagtcaa ctctacttgg cagcaaggag cgtcaataac
     5761 ataccgaggt gtatgggatg gcaagcctta tgaggacaag ggcgtgattc aagatatcac
     5821 acctgaagtg ctgctaaaga caacgtactt tagtccactt tccggcaagg aggacatctc
     5881 cgagaattat aatcttgtta cttatgttat agcccgaaac gatgacggaa caagcggtct
     5941 atctgtaaca caagaaaata atgactcaca ggaaaccgcg gataatatga agggcaactg
     6001 gcagcaaatg ctcgatggac ttgctgcata tatcacaagt tcgaaaacta tgtccaagtc
     6061 gtagccgatt ggagaaccga gctggaaact tgtaatcgta taacatggag gcacgtacgg
     6121 gagttgcacc cgtctacgcg gttttgcaga ccgctgcata agtgctccgc caacgcgcca
     6181 tctaggacat tatagcagat ggaatatggc tcaatagaat tgctaaccca tgcccgctgt
     6241 agctgtaaca cctcttgcaa acagagacca agtctggtat attttgatag tacattcatc
     6301 cctatgcact
//
LOCUS       DATIIX010000113         3082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_527399, whole genome shotgun sequence.
ACCESSION   DATIIX010000113 DATIIX010000000
VERSION     DATIIX010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527399"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1560
                     /gene="glmS"
                     /locus_tag="VLF64_03105"
     CDS             <1..1560
                     /gene="glmS"
                     /locus_tag="VLF64_03105"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HSW90961.1"
                     /translation="VGDIYLVHNGIIENYQDLKAELGKYDYDFKSDTDSEVLAALVDY
                     IYKKDDTTLLDALSQALKMVVGTYGIAVMSIKNPNEIIIARKGSPLIVGVGEGEVFIA
                     SDASALVGYTNQVVYLHDGEVGVCSRDGVQLYDIDANEVDAAVETLELDLQAIQKKGY
                     DHFLLKEINEQPDSLRSTLSGRVNADNLYARLGGLNMTNAELRDIEHVMVIGCGTAYY
                     AGLEAGYLVEQFLDNVTFSSQIASELRYRSFAVPKNTVALIVSQSGETADTLACLQEL
                     KRRGVKCLGVVNVVGSTIAREVDGGVYVHVGAEISVASTKAFTSQVAAITIFGLMVAQ
                     AKGMGSKELSSYIRELELLPSEIEKVIAKHDKEVKKIASTYAKYDHALFVGRDALFPI
                     ALEGALKLKEVSYVHAEGYAAGELKHGPIALIDDRFFEVCYLQDNWLYEKSQSNLIEM
                     NARGAHAIVITNTSKRVPAEQVIRVSTKLQLFTPLVFNVISQLLAYYVAVARGNDVDQ
                     PRNLAKSVTVE"
     gene            complement(1561..2703)
                     /locus_tag="VLF64_03110"
     CDS             complement(1561..2703)
                     /locus_tag="VLF64_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90962.1"
                     /translation="MESLLVGQNDVERLNAEPATGPGTPIAQDPPQNIQPQMAPPSAK
                     PPLDMPKKSHKKRYIIIATLLLLLLTAGAIAAIMIFSAPKKDTGRKFASQATKPLDDA
                     QGMITKINTALTDSSNTGYQNPQISFTSPTSTPPAVSPSYQVSGADYYVSVTQAYGLN
                     IQKTVDTNSETGVDTVFTQGVIDEMTSAFVSSRYHKTIDLPSGVEYQSDAVICTVSGT
                     NSIPIYVTCANKNAYIKVSSDVKPFVTAYKASNSNDASQSVFSAPIIKSSSTSGYQTA
                     GINMWSRQSAGGAGLIFYRKNNGDWKFFTVTQNELSCSKYNTDDLKAAFADESCMSDA
                     NVDTTVKNYVPGAKKVSGSSSSSLSNSTTKNDTTYNSGAASSLTNP"
     gene            complement(2737..>3082)
                     /locus_tag="VLF64_03115"
     CDS             complement(2737..>3082)
                     /locus_tag="VLF64_03115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSW90963.1"
                     /translation="DRFGDASNSEVEEALNNAKGFIYPAEEDFGIVTVEALAAGAPAI
                     ALAKAGTLDIVQDGETGILFEHQTVEDVVTAIKRAEEITFFPSKLQRTAKRFNTSLFI
                     TKLRKVVTDQIH"
BASE COUNT          807 a          703 c          758 g          814 t
ORIGIN      
        1 gtcggcgata tttaccttgt acataatggt attattgaaa actaccaaga tcttaaagct
       61 gaactcggta agtacgacta cgacttcaaa agtgacaccg acagcgaagt actcgcggcg
      121 ctcgtagact atatttacaa aaaagacgac accaccctgc tcgatgcact gagccaagcg
      181 cttaaaatgg tagtaggaac ctacggtatt gcggtcatga gcatcaaaaa cccaaatgag
      241 attatcattg cacgtaaggg cagcccgctt atcgttggtg ttggcgaggg tgaagtgttt
      301 attgcaagtg acgcttcggc actcgtcggc tacaccaatc aagtggtgta cttacatgat
      361 ggagaggttg gtgtttgctc gcgcgacggc gtacagcttt acgatatcga tgcgaacgaa
      421 gttgatgccg cggttgaaac acttgagctt gacctgcagg caatccagaa aaaggggtat
      481 gaccacttcc ttctaaaaga gattaatgag caaccagata gtttgcgcag cacccttagc
      541 ggccgtgtaa atgccgataa tctgtatgca cggcttggcg gtcttaatat gaccaacgcc
      601 gaactacgcg atattgaaca cgtaatggtg atcggctgtg ggacagccta ttatgctgga
      661 cttgaagcag gctatttggt cgagcaattt ctcgataacg taaccttcag ctcgcaaatt
      721 gccagtgaac tccgctatcg ctcatttgca gtgcctaaaa acacggtagc tttgattgta
      781 agccagagcg gcgagaccgc tgatacgctt gcttgcctgc aggagctaaa gcgtcgtggg
      841 gtaaaatgtt tgggtgtcgt gaatgtagtg ggcagcacga ttgctcgtga ggtcgacggc
      901 ggtgtatatg tacatgttgg cgccgaaatc agtgttgcca gcactaaggc ctttacctca
      961 caggtagcgg caatcactat ttttggactc atggtcgcac aggcaaaagg catgggctcg
     1021 aaagaactca gtagctacat tcgcgagctc gagctattgc cgagcgaaat cgaaaaggta
     1081 atcgcaaagc acgataaaga agtaaagaag attgcgagca cctacgcaaa atacgaccac
     1141 gcactgtttg ttggtcgcga tgcactcttc ccaatcgcac tcgaaggcgc gctcaaactg
     1201 aaagaagtga gttatgtaca cgccgaaggc tacgcagcag gtgagctcaa gcatgggcca
     1261 atcgcgctca tcgacgatcg attcttcgag gtttgctacc tgcaagataa ctggctatac
     1321 gaaaagagcc aaagcaatct cattgagatg aatgctcgcg gtgcacacgc aattgttatt
     1381 accaatacca gcaaacgagt cccagccgag caagtaattc gggtaagtac aaaattacag
     1441 ctgttcactc cacttgtatt caatgttata tcgcagcttc ttgcctacta tgtagcagtc
     1501 gcgcgtggca acgatgtaga ccagccacgc aacctcgcaa agagcgtaac cgtagaatag
     1561 ttacggattt gtcaaagatg acgcggcccc agagttgtag gtcgtgtcgt ttttggttgt
     1621 tgagttagaa agtgaacttg agctcgaacc tgagactttc ttcgcaccag gaacataatt
     1681 tttaacagtt gtatcgacat tagcatccga catgcatgac tcatcggcga acgcagcctt
     1741 aagatcatcg gtattgtact tactgcaact taattcattt tgcgttacag taaagaattt
     1801 ccagtcacca ttattcttac ggtaaaagat aagacctgcg ccaccggcgc tttgtcgaga
     1861 ccacatattg attccagccg tctggtagcc gctagtgctg ctgcttttaa tgattggtgc
     1921 agagaaaaca ctctgcgaag catcgtttga gttgctcgct ttataggcgg taacaaatgg
     1981 cttaacatcg ctgctcactt ttatgtatgc gtttttgtta gcacaggtta cgtagatagg
     2041 aatactgtta gtgccagata ctgtacaaat aacagcatct gactggtatt cgacccccga
     2101 tggaagatct attgttttgt ggtatcggct gcttacgaac gcagaagtca tttcgtcgat
     2161 gacaccctga gtgaatacag tatccacgcc tgtttcgcta ttagtgtcga cagttttctg
     2221 gatattgagt ccgtacgctt gagtaacact cacatagtaa tcagcaccac tcacctggta
     2281 gctagggctt acggcaggtg gggtacttgt aggtgacgta aaactaattt gcggattctg
     2341 gtagccggta tttgaactat ccgtcagcgc tgtattaatc tttgtgatca taccttgggc
     2401 gtcatcaagt ggcttagtgg cctgtgatgc aaactttctt cccgtatctt tctttggtgc
     2461 agagaagatc ataatagccg caatagcacc ggcggtaagt aatagcaaaa gtagggtcgc
     2521 aataataata tagcgctttt tatggctctt ttttggcatg tcgagcggag gctttgcaga
     2581 tgggggagcc atctgcggtt ggatattctg aggtggatcc tgagctattg gtgttcctgg
     2641 gcctgtagca ggctcggcat taagcctctc aacatcattt tgccccacca gtaaactctc
     2701 cataagtaaa ctatagcatt atggtgagct attgtgctag tgaatttggt cggtaacaac
     2761 cttgcgcagc ttagtaataa acagtgacgt attaaaccgc tttgcggtgc gctgtaattt
     2821 cgacgggaag aacgtaattt cctcggctcg cttgattgcg gtaaccacat cctcgaccgt
     2881 ttgatgctca aacagtatgc ctgtttcgcc gtcttgcacg atatctagcg tgccagcctt
     2941 tgcaagtgcg atcgcaggtg cacccgcggc gagtgcctcc acagtaacaa tcccgaaatc
     3001 ttcctcggct gggtaaatga atcctttagc attattaagt gcttcttcaa cttcactatt
     3061 cgatgcatcg ccaaatcgat cc
//
LOCUS       DATIIX010000114         4841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_553161, whole genome shotgun sequence.
ACCESSION   DATIIX010000114 DATIIX010000000
VERSION     DATIIX010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553161"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VLF64_03120"
     CDS             <1..444
                     /locus_tag="VLF64_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HSW90964.1"
                     /translation="FDPFAELNALQRQFFGDDWFTPIKGGAIPTTDVYTRGGKELVVE
                     AHLPNFDQEDIDIHVDDNGSALVIQAEKHEREEDKDKKYVVRESSNSFYRRIALPERA
                     DGDSVEAHLDGGVLKVTVPLKSLPEPKKVTIQSGDSNGHKKLTNK"
     gene            641..1351
                     /locus_tag="VLF64_03125"
     CDS             641..1351
                     /locus_tag="VLF64_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSW90965.1"
                     /translation="MSSRPNDTLIHITDISKKYRRGHESVDALHSISLDIYKGELLAI
                     VGPSGSGKTTLTHIVGGLITPDTGTVEVEGRPLKGRRDKQLSSFRNSEVGFVFQNFSL
                     IPYYTVLENVTLPLVVAKMAPRERRELAKEYLKLVSLEKRIHQRANELSGGERQRVAI
                     ARALVNHPQIIIADEPTGSLDSARGNEIMAILETLSHTRGITVLVVTHDTKLAARADR
                     SIHIQDGHLIKEESHANQ"
     gene            1338..2675
                     /locus_tag="VLF64_03130"
     CDS             1338..2675
                     /locus_tag="VLF64_03130"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSW90966.1"
                     /translation="MRTSDYLMVALRDIRRQPLRSGLTIFALIISTVILVLMAAISVG
                     GRQAITEQFGPSSGLTSIAVTPNQSNSSLSPFGGIQEVNSHAAKLDDTTVTTLAKLPH
                     VNSASPQAHIWELANFSLDGSDKQFVAQAEGVPSDAKLPLTAGVAFTSNDQANVAIIG
                     LSYAKALGFGDNPTQLIGKTINVTTQKGYRGIGATIPDALATKTTLDAFNQQATTLKV
                     QIVGVTTTGPNQNELFVPIGWAHAIRTIHYYDAGMSKQTDQIAENGYTTIKVMVDGTA
                     NVASVSSAIEALGYGQVSTLSQVQRLQQLSATMWMILGAVAIIAVVAAALGVVNTMLM
                     AVSEQRYTIGVWRACGARRRTMVALFLTESALLGFIGGALGVAIGVFAGRFVNEYVGV
                     LLQNQGFTLTNIAIVPPWLVGGTIMVTTIFGIIAGAYPAYRAARQDPVQALTE"
     gene            complement(2676..4172)
                     /locus_tag="VLF64_03135"
     CDS             complement(2676..4172)
                     /locus_tag="VLF64_03135"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSW90967.1"
                     /translation="MKKSPLLYIVIIACWIALTAAAITPFMKAVHGSESHGTLVLVLV
                     SLSTAFVVYFWLNGTKDLVYTFYYFLRRKQFDLPLEGEWRRKNGRDASKRIVAVYCTY
                     NDFNGASLAACMTQDYPNTTYVILDDSSQKSYMDEVDEFARLHNIEVVRRENHVGFKA
                     GNLNNYLQRGGFDYFVILDSDEVIPGNFVTRCLDYFAYYENAGIVQANHVATRNRNKF
                     MRLFAIGVNSHWPTYQTVKHYNGFLSLLGHGAMVSKECYEAAGGFPHLVAEDLCMSIE
                     ARNKGFYTAFAPDITCEEEYPISYLAFKKRHSKWTQGNMEFNKKYTWRIATSKMTWYE
                     KLDIVLFTYSLPLSAFFALYIIINVILLPVLHYHITYPVWLLIPTLMFLFAPMLNDII
                     FYGRKIKPHQLVWYLLHTVLLYGSMLFTSLRASFKSMFGKSIFVVTPKEHERLRRWDA
                     LKANKGELLFGIAVLIIAFVFNQTIASTVIIAIPGLFSIYLSLMANNE"
     gene            complement(4248..>4841)
                     /locus_tag="VLF64_03140"
     CDS             complement(4248..>4841)
                     /locus_tag="VLF64_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HSW90968.1"
                     /translation="LQQDVEPDCAFIVKSGTVKTYNITASGEEKPIGFSLKNEIFPIG
                     WVFSKIFRSQYYYEALTDSEVYVVPKDDLITYLRKTPEAMLQLLGQCVDDALHNQMRI
                     NALEQSKAYEKVLHTIHYLALCFGRDLRQDLVEIPLPLTQQDVANFTGLTRETISVEL
                     KKLAHQKIIMYRNRCYVVLTDKLDDLLDDDYEHQFVR"
BASE COUNT         1312 a         1148 c         1167 g         1214 t
ORIGIN      
        1 ttcgatccct ttgcagaact aaacgcgcta cagcgacagt tcttcggtga cgactggttt
       61 acgccaatta aaggtggcgc cattccaact acggatgtct acacaagggg tgggaaagaa
      121 ctcgtcgttg aggcgcatct gccgaacttc gaccaagaag atattgacat tcacgtggac
      181 gacaatggga gtgctctcgt aattcaggct gaaaagcacg aacgcgaaga ggataaagat
      241 aaaaagtatg tcgttcgtga aagttctaat agcttctatc gtcggattgc actcccggaa
      301 cgcgcggacg gtgatagcgt cgaagcccat cttgatggcg gtgtgctcaa ggtgacagtg
      361 ccacttaagt cgcttccaga gcctaaaaag gtgacgatcc aaagcggcga tagcaatggg
      421 cataagaaat tgacaaacaa gtagtcagca taggctgcag taaagatagt ccggcccacc
      481 gtggaggtcg ggctattgct ttgtgtatga tcacgcatag tgacccaacc cgacaattat
      541 cgtaacgccg ccgggatgaa aagcgccatt ctttctcctc agctgaggag aaagctactt
      601 gttattggta ctgctttgca aagtcctata tactaaaaag atgagtagta ggcctaacga
      661 tacacttata cacatcactg atatatctaa aaaataccgc cgggggcacg aatcggtaga
      721 tgcgcttcat agtatctcat tggacatcta taaaggtgaa cttttggcca tcgtgggccc
      781 ttccgggtca ggcaaaacga cattaacgca tattgtgggt gggctaatca ccccagatac
      841 gggcacagtg gaagtggaag gccgtccgct aaaaggacgt cgagacaaac agctttcgag
      901 ctttcgtaat agcgaagtcg gttttgtatt ccaaaacttc agccttattc cgtattacac
      961 tgtgctcgaa aatgtcacgc tgcctctagt ggtagcaaaa atggcaccgc gcgagcggcg
     1021 cgagctcgct aaagaatacc taaaactagt cagcctcgaa aaacgaatac accagcgcgc
     1081 caatgaatta tcaggtggtg agcgccagcg tgtagcgatt gctcgggcac tcgtgaacca
     1141 tccgcagatt attatcgccg atgagcctac gggtagtttg gattctgctc gtggcaacga
     1201 aattatggca atcctcgaaa cgctcagcca cacgcgtggc attactgtgc tggtggtgac
     1261 acatgacaca aaacttgcgg cgcgcgccga tcgaagtatt cacattcaag acgggcacct
     1321 tataaaggag gaatcacatg cgaaccagtg attaccttat ggtcgcgcta agagacattc
     1381 ggcgacagcc gctgcgctct ggtcttacta tattcgctct gatcattagt acggttattt
     1441 tggtattgat ggcggcaatt agtgttggtg ggcggcaagc aatcacggag cagttcggcc
     1501 ccagtagcgg tcttacaagc atcgccgtaa cgccaaacca aagcaactcg tcgctcagtc
     1561 cattcggcgg tatacaagaa gtgaatagtc acgccgcaaa acttgacgat acaaccgtca
     1621 ccacgctcgc aaagctgcca catgtcaact cggcgagccc gcaggcgcat atatgggaac
     1681 tcgccaattt ctctcttgat ggcagcgaca agcaattcgt cgcccaggcc gaaggtgtac
     1741 caagtgatgc caaactaccg cttacggctg gtgtcgcgtt tacctcaaat gatcaggcaa
     1801 atgtcgcaat tattgggcta agttatgcaa aagcactcgg atttggtgat aacccgacac
     1861 agcttattgg taaaacgatc aatgtcacaa cgcaaaaagg ctatcgcggt ataggtgcaa
     1921 ctatccccga tgcactagct acgaagacca cgcttgatgc gttcaatcaa caagcaacca
     1981 cgctaaaagt gcaaatcgta ggcgtcacaa caaccggccc aaatcaaaat gagctattcg
     2041 tgccaatcgg ttgggcacat gcaatcagga caattcatta ttacgatgcc ggcatgtcga
     2101 agcaaacgga tcagatcgca gaaaacggct acactacaat aaaagtaatg gtggacggta
     2161 ctgcgaatgt ggcgtctgtg tcatcagcta ttgaggcgct tggctatggg caggtctcta
     2221 cactatcgca ggtgcaacgc ctgcagcagc tttctgcaac aatgtggatg atattaggcg
     2281 ccgtagcaat cattgcggtt gttgcagctg cgcttggcgt cgtaaacaca atgcttatgg
     2341 cggtatcaga gcagcgctac acaattggcg tatggcgtgc atgtggcgct cgcaggcgaa
     2401 caatggttgc acttttcttg accgaatccg cgctccttgg ctttattggt ggcgcgcttg
     2461 gcgtagcaat cggtgtgttt gcgggccgct tcgtaaacga atatgttggt gtgcttctgc
     2521 aaaaccaagg attcacactt actaatatag caatcgtacc accgtggcta gtgggtggta
     2581 cgattatggt aacgacgatc tttggtatta ttgccggcgc ataccctgca tatcgtgcgg
     2641 cccggcagga ccccgtacag gcgctcactg aataactatt cgttattagc catcagcgat
     2701 aagtaaatac taaatagtcc cggtattgcg atgataacgg tcgatgcaat cgtttgattg
     2761 aatacgaagg cgataatgag gacggcaatg ccaaacagca gttcgccctt attcgctttt
     2821 agcgcatccc atcgccgtaa gcgctcgtgc tctttcggcg tcacaacaaa gatggattta
     2881 ccaaacatcg atttaaacga cgcacgcagg ctcgtgaaca gcattgatcc atacaaaagc
     2941 accgtgtgca gcaaatacca taccagttgg tgtggtttga tcttacggcc ataaaatatg
     3001 atgtcattga gcatcggcgc gaacaaaaac atcagggttg gaatcaatag ccatactggg
     3061 taggtgatgt gataatgcag cacgggcaac agtatcacgt taatgatgat atagagggcg
     3121 aaaaatgcag aaagcggtaa actgtaagtg aatagcacga tatccagttt ttcgtaccac
     3181 gtcatttttg atgtagcaat gcgccatgtg tactttttat tgaactccat attgccttgg
     3241 gtccactttg agtggcgctt cttaaacgct aggtaactaa ttgggtactc ttcttcacag
     3301 gtaatgtcgg gtgcaaatgc tgtgtagaag cccttgttgc gcgcttcgat gctcatgcat
     3361 agatcttcgg caaccaggtg tgggaatcct cccgccgcct cgtagcactc tttgcttacc
     3421 attgcaccgt gtccgagcag tgacaagaac ccattgtaat gtttgaccgt ctgataggtt
     3481 ggccagtgtg aattaacacc gattgcaaag agacgcatga atttattacg gttgcgcgtt
     3541 gccacatggt tggcctgcac gataccagca ttttcatagt aggcgaagta atcgaggcac
     3601 ctcgttacaa aattgcccgg gatcacttcg tcactatcta aaattacaaa gtaatcaaaa
     3661 ccaccgcgct gtaggtagtt atttaaatta ccagctttaa acccaacatg gttttcacgg
     3721 cgaactactt ctatattgtg aaggcgtgca aactcgtcta cttcatccat gtacgacttc
     3781 tggctcgaat catcgagtat gacgtaggta gtgtttgggt agtcctgtgt catgcaggca
     3841 gccaggcttg ctccattaaa atcgttgtag gtgcaatata cggcgacaat tcgcttgctt
     3901 gcatcccttc cgttcttgcg tcgccactca ccttcgagtg gcaagtcaaa ctgcttgcgg
     3961 cgtaaaaagt aataaaaggt atacacgaga tcttttgtcc cgttcaacca aaaatacacg
     4021 acaaatgccg tgctaagact cacgaggacg aggacgagcg taccgtgact ttctgaaccg
     4081 tgcactgctt tcataaatgg tgtaatcgct gctgctgtca gtgcaatcca acaggcgata
     4141 atgactatgt acagtaatgg tgacttcttc atcaatctcc tttttcaacg ttaccgcagg
     4201 taagcgtagc acacgggaaa atgacaaaaa tatgaattat ttcacaatta tcgaacgaac
     4261 tgatgttcgt aatcgtcgtc caataaatcg tcgagcttat ctgttaatac gacataacaa
     4321 cgattgcgat acataataat cttctgatga gcgagttttt ttagttcaac acttatcgtt
     4381 tcgcgcgtaa gtcctgtaaa atttgcaaca tcttgctgcg taagtggcaa cgggatctct
     4441 acgagatcct ggcgcaggtc gcgcccaaaa caaagcgcta agtagtgaat tgtatgcaat
     4501 accttctcgt aagcctttga ttgctcaagg gcattaatac gcatttgatt gtgcagcgca
     4561 tcgtcaacgc attggccgag aagctgcagc attgcttcgg gcgttttacg aagatacgtt
     4621 atcaaatcat ctttcggtac tacatacact tcgctatccg tcagtgcctc gtagtaatat
     4681 tgggatcgaa agattttact aaatacccat ccgatcggga agatctcgtt cttaaggcta
     4741 aacccaattg gtttctcctc accgcttgcc gtgatattat aggtttttac cgtgccgctc
     4801 tttacgataa aagcgcaatc cggttcgaca tcttgctgca a
//
LOCUS       DATIIX010000115         8351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_26157, whole genome shotgun sequence.
ACCESSION   DATIIX010000115 DATIIX010000000
VERSION     DATIIX010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 8351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8351
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26157"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1576)
                     /locus_tag="VLF64_03145"
     CDS             complement(<1..1576)
                     /locus_tag="VLF64_03145"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HSW90969.1"
                     /translation="MARKSNVDGFIPRRSPRAVGGDYVSRRSPSPMAQGLRQRPIISD
                     TRSATSVGSEGLTRSDIDESLNEIDTQAPLTRKERKQQKKHQKAPKSRRRKVIKRVII
                     ALVIILLAIGGYVAFKAMSASNSVFKGDIFGLVQQKKLQQDANGRTNILILGTSEDDP
                     GHQGAYLTDSIMVLSLDQANKNAYMISIPRDMQVQYGMACDAGYAGKINVYFNCVNSD
                     WGSESAETERQAKSREFYGNILGIPIQYSAHVNYSVMRDVVNAVGNITVNIQGSGGAP
                     GVMDSNFDWKCKGGNAHASLATMKQNCPPSGHFIDYPNGPATLDAEHALYLAQARGDV
                     APTYGLGNSNFDREQNQQKIVLAIKEKATGTGTLTNIGKITGLIDAIGKNLRTNFETS
                     EVRTLMTLAKDIPSSSFQSVNLLDGGIMTGDAQPVAGLYNFTQLQAFVKKMIYATGIT
                     KEGAHVIVLNASGVAGAAQTEADKLTALGMTVDTVDNAPTGGAYVSNEIFKLTDSKMP
                     LTTAKLESLYGAKIQQV"
     gene            1675..2286
                     /gene="lepB"
                     /locus_tag="VLF64_03150"
     CDS             1675..2286
                     /gene="lepB"
                     /locus_tag="VLF64_03150"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HSW90970.1"
                     /translation="MQSNYLTRHPHAKDALQFVGFIILVFIGTLVINTFIFRSFSVLG
                     HSMDKTLADGQRLIVNRLPITIAQAENKSYMPDRGQIIVFKNPRYVAGMPDEYIVKRV
                     IAFPGERVTVHDGVLKVYNNDHPDGFNPDTAYRKDGVGPQSPVSGEGLDTVVPDDTVF
                     VCGDNRIGDNSYDSRTGLGNIPLFDIVGPVSFRIWPFNAIHTF"
     gene            complement(2341..3228)
                     /locus_tag="VLF64_03155"
     CDS             complement(2341..3228)
                     /locus_tag="VLF64_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HSW90971.1"
                     /translation="MPAKKKGLGRGFESLIPTELFDESFDPTAEQDDRVSDLRHIKLS
                     EIVADPDQPRRSFDEVSLEELGASIREHGVLQPIVVAPHEGKYMIVAGERRFRAATKV
                     GLEKMPALVRTLSNQHKLEISLIENLQRRDLNPLETATAYLKLRDQFNLTLDQIGERV
                     GGKTPGAVSNTLRLLRLPEVVRNALAAGTVREGQVRPLIGLDTKIVEAILPRIIREEW
                     SARDTERYANQVKNGEIAARRSEAKPVSAKYVDRATKIAKHFNAKVSVRQMARGKGQI
                     VITYKNEDDFERLAKLLEK"
     gene            complement(3218..3982)
                     /locus_tag="VLF64_03160"
     CDS             complement(3218..3982)
                     /locus_tag="VLF64_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HSW90972.1"
                     /translation="MSIIIAVTNQKGGVGKTTSSINIAYYLAKAGKRTLLVDFDPQGN
                     ATSGLGVDKTELDVTVADVVTGQKKLAEVILPTEFKNLSIAPAMPTLANTEVEIAQAE
                     HRFSRLKYAIESLNTEYEYVIIDCPPSLSLLTVNALIAAKYVLLPVQAEFYALEGLGQ
                     LLETMKLVRKNLNPTLDLIGVLPTMVDSRTSLSNQVLSEIAKHFPAKVFKTTIPRNVR
                     LAEAPSHGVPIGAYDRFSKGARAYKAAAKEVMERAS"
     gene            complement(4072..4377)
                     /gene="rplU"
                     /locus_tag="VLF64_03165"
     CDS             complement(4072..4377)
                     /gene="rplU"
                     /locus_tag="VLF64_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HSW90973.1"
                     /translation="MKAVVKVGGKQHIVAEKETLMVDLLPEGTKELDLDALLLIDGDK
                     ITVGAPFVKGVKVKAKVVDEKVAGEKIRVIRYKSKKRVNTQTGHRQKYTKIEIVSIK"
     gene            complement(4540..4788)
                     /locus_tag="VLF64_03170"
     CDS             complement(4540..4788)
                     /locus_tag="VLF64_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90974.1"
                     /translation="MLHLSPSRFSFFQSQPAQHVELSAILDDDNDELIQAIKADHEET
                     WELDPSPDTTALSKFWSGVEDDLHNDPDWVTFTEDDES"
     gene            complement(4892..7741)
                     /gene="ileS"
                     /locus_tag="VLF64_03175"
     CDS             complement(4892..7741)
                     /gene="ileS"
                     /locus_tag="VLF64_03175"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HSW90975.1"
                     /translation="MKFQHGTRRRALEYEKDRVQRWKLESTFEKSVENRPAGDAYVFY
                     DGPPFISGVPHHGTLLSSIVKDAVPRYWTMKGKRVERVWGWDCHGLPAERFSEKKLGL
                     SSHQDVLDYGLEKYIIATRAYMTQTAADWEDVIDRIGRWVDFNGAYKTMDKNYMESIW
                     WAFKELYEKGKIYEGEKVLMYCTLDGTPLSKAEVTMDAGAYQDVTDPSVFVKFKLENG
                     VTLLAWTTTPWTLPANTAVAINKDFTYVEVAVAGEKLVVAKDLVAKVFVNEKHQPLEY
                     TALRELKGKDLVGLSYEPLLGVNRGKNAHKVWDAEYVSDEDGTGIVHLAPAYGEEDFE
                     LAREKKIPVVHVLDENGLYTEGDWLGRNVWEENKAIAKTLKERGIVWKIEYIRHSYPH
                     CHRCGTRLMYRAHPSWFANIADQKTQMLEQNGNVNWFPAHLKNGRFAKNIEQAPDWNL
                     SRDRFWATPLPVWKTKSGKIKVVGSYAELKELSGIELEDYHRPWIDEVKFTIDGEEYA
                     RIDKVMDSWFEAGSMPFAQFHYPFENKEKFEANFPGDFIVEYVGQVRAWFYYMHAMSV
                     ALFGTNSFKNVITTGTLAGNDGRKMSKSYGNFTDPNELMDKFSADSLRFLLLSSPVLN
                     GEDFALHDKDVGDVARKLSMVWNMYDFFTMYAEVDGWEFDPKQAQTLSIAEVTNPLDK
                     WVISRVHQLNEHITHNMDAYNLPDAMAEILPFIEDASNWYVRRSRRRFWKSEETSDKN
                     DAYRTLHYVLLKLSLILAPFTPFLAEELYQNLGGDSESVHLLDWPTEFGIDQLVLDEM
                     EQVRGYVNEGLSLRAKAGLKVRQPLASVAVPSLGKFVDYKAILTDELNVKIVKLGGGV
                     AIDEKLTPVLKREGLMREVIRYVQSARKKAGLNVDDHIKLFLVTDDKELSKAIDEHQE
                     EIYAETLAEGGTKAGDYTEEVTVEGVKLRVSLSKR"
     gene            complement(7738..8265)
                     /locus_tag="VLF64_03180"
     CDS             complement(7738..8265)
                     /locus_tag="VLF64_03180"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSW90976.1"
                     /translation="MTEQPQFIITELTAATIDESNRMRLQLWLDTYVNDEAGVTREWI
                     EARNVDQMSDEKRARRLERLDSSKTAGWVAFDLDSRVIGMATPYVDDEGRQHVGSLYT
                     DKNRHGKGVGGALMQKIVNWSNPKKPIELGVVTYNERAKAFYRKWGFEEIPGSETLFD
                     NTIPEIMMIRKGDRQ"
BASE COUNT         2146 a         2229 c         1651 g         2325 t
ORIGIN      
        1 tcacctgttg tatctttgca ccgtacagac tttcaagctt tgcagtcgtc aaaggcattt
       61 tactatcagt aagcttaaaa atttcattgg acacatatgc tccacctgtt ggcgcgttgt
      121 caaccgtatc gacagtcatg ccaagtgccg taagcttatc tgcttctgtc tgagcagcgc
      181 ctgctacacc tgaagcattc agtacaataa catgcgcacc ttccttggta atacccgtcg
      241 cgtaaatcat cttcttcaca aacgcctgaa gctgcgtaaa attatacagc cccgctacag
      301 gttgcgcatc accagtcata atgccaccat cgagtaagtt aacgctttgg aatgagctcg
      361 atggaatgtc ttttgcaagg gtcataaggg tacggacttc actcgtttca aaattagtgc
      421 gcaagttctt gccaattgcg tcaatcaatc cagtaatctt accgatattt gtgagcgtcc
      481 cggtaccagt agctttttct ttaatagcaa ggacaatctt ctgctggttc tgctcacggt
      541 caaaattaga attaccaaga ccataagtag gagcgacgtc gccacgcgcc tgtgccaagt
      601 aaagcgcatg ttctgcatca agcgttgctg gaccatttgg ataatcaata aagtggccgc
      661 tcggtggaca gttctgcttc attgtcgcaa ggcttgcgtg cgcattacca cccttacact
      721 tccagtcgaa attactatcc attacacctg gtgcgccacc gctaccttgt atattcacag
      781 taatattacc gaccgcattt actacatcac gcatcaccga gtaattcaca tgagcactgt
      841 attgaatagg aatgccgagt atattgccat agaattcacg tgactttgct tgacgctcag
      901 tttccgcaga ttcagagccc caatcactgt ttacgcagtt aaagtacaca ttaatcttcc
      961 cggcatatcc tgcatcacac gccataccat attgcacctg catatcacgc ggaatactta
     1021 tcatatatgc attcttattc gcctgatcaa ggcttagcac cataatagaa tccgtcaaat
     1081 acgccccttg gtgccccgga tcgtcttcag acgttccaag aattaagata ttcgtacgcc
     1141 cattagcatc ctgttgcagc ttcttttgct gtaccaaccc gaaaatgtcc cccttgaata
     1201 ccgagttact cgccgacatt gccttaaaag ctacatatcc accaatagca agcaaaataa
     1261 tgacaagagc aattattaca cgcttaataa ctttacggcg gcgagacttc ggcgcttttt
     1321 ggtgcttttt ctgctgctta cgctcttttc tcgtaagagg cgcttgggta tcaatctcgt
     1381 tcagcgattc gtcgatatcg ctgcgcgtta aaccttctga tccaacagat gttgccgaac
     1441 gggtatcaga aataatcggt cgctgccgca gcccctgtgc catcgggctc ggactgcgac
     1501 gactaacata atcaccacca accgcacgcg gggagcgtcg aggaataaag ccatcaacgt
     1561 ttgattttct cgccattact tttatccact atagccgatg ctagacaaaa aataaacata
     1621 ccgcctacgc taaatggttt tattttttac cttaaacgcg ctatagtgag tgcaatgcaa
     1681 tcaaactacc taactcgaca tcctcatgcc aaagacgccc tccaatttgt tgggtttatt
     1741 atcttggttt ttatcggcac gctcgttatt aatacgttca tcttccgcag cttctcagta
     1801 ttagggcata gtatggacaa gacacttgcc gatgggcagc gactcattgt taatcgcttg
     1861 ccgatcacta tcgctcaggc cgaaaacaaa tcatatatgc cagatcgcgg tcaaatcatt
     1921 gtgttcaaga acccgcgcta tgtagcaggc atgccagacg aatatatcgt caagcgagtc
     1981 attgccttcc ctggtgaacg cgttacagtt catgatggtg tacttaaagt gtacaataat
     2041 gaccaccctg atggattcaa cccagatacc gcctaccgta aagatggcgt tggaccgcag
     2101 tcaccagtca gcggcgaagg tctcgatacc gtagtgccgg atgatacagt gttcgtttgc
     2161 ggagacaacc gcattggtga taactcatac gactcacgca caggccttgg taacattcca
     2221 ctcttcgata ttgttggccc tgtgtcgttc cggatttggc cattcaatgc tattcataca
     2281 ttctagtctc tatacacata aaataacccg ccatatcggc gggttatttt attcaatact
     2341 ctatttctcg agcaacttcg caagccgctc aaaatcatcc tcgttcttgt acgtaatcac
     2401 aatctggccc ttgccacgcg ccatctggcg cacgctcacc ttcgcgttaa agtgcttagc
     2461 aattttcgtc gctcgatcca catactttgc agacaccggc ttagcttcac ttcgtcgagc
     2521 cgcaatctca ccattcttaa cctggtttgc ataacgctca gtatcgcgcg cactccattc
     2581 ttcacggata attcgcggca gaatcgcctc tacaatcttt gtatcgagtc cgatcagtgg
     2641 acgaacctga ccctcgcgta ccgtcccagc tgcaagtgcg ttccgcacta cctctggcag
     2701 acgaagcaaa cgcagtgtgt tacttaccgc ccctggcgtt ttaccgccaa ctcgctcacc
     2761 aatctggtca agagttagat taaactgatc ccgcaacttc aagtacgccg tagccgtttc
     2821 aagcgggtta aggtcgcgac gctgtaggtt ctcaattaat gatatctcga gcttatgctg
     2881 gttagaaagt gtgcgtacaa gtgccggcat cttttcgagc ccgaccttgg ttgcggcacg
     2941 aaatcggcgc tcaccagcaa cgatcatata cttgccctca tgtggcgcaa caacgatagg
     3001 ctgcaacacg ccatgctctc ggatggaagc accaagttct tccaaagaaa cctcatcaaa
     3061 cgaacggcgt ggctgatcag ggtcggcaac aatctcactc agcttaatat gacgcagatc
     3121 actcacgcga tcatcttgct cagccgttgg atcgaacgac tcatcaaata gctcagtcgg
     3181 aataagtgac tcgaatcccc ttccaagacc tttcttttta gctggcacgt tccatcacct
     3241 ccttcgcagc tgctttatac gcccttgcac ccttgctgaa tcgatcatat gccccaattg
     3301 gcacaccgtg gcttggcgcc tcagcaaggc gtacgttacg gggaattgtc gtcttgaata
     3361 ctttagctgg gaaatgcttg gcaatttctg aaagcacctg atttgataac gatgtgcgac
     3421 tatctaccat cgtcggcaac acaccaatca ggtcaagagt agggttaagg ttcttacgca
     3481 caagcttcat cgtctcaagc agctgaccaa gcccttcaag cgcgtaaaac tcagcctgca
     3541 ctggtaataa cacatatttc gcagcaatca gcgcatttac cgtcaataag ctcaaagaag
     3601 gtggacaatc gataataacg tattcatact cagtattcag cgattcaatt gcatatttca
     3661 atcgactaaa tcgatgttct gcctgcgcga tttctacctc agtattcgca agtgtcggca
     3721 tcgctggcgc gattgaaaga ttcttaaatt cagttggcaa aatcacctct gcaagtttct
     3781 tctggcctgt gaccacgtcc gccacggtca catcgagctc tgttttatca actccaaggc
     3841 cactcgtcgc attaccctgt ggatcaaaat caactaagag cgtccttttg ccagctttgg
     3901 ccaaatagta cgcgatgtta attgagctcg ttgtcttacc gacgccgccc ttttgatttg
     3961 ttacggcaat aattatactc accttttgtg tcttccctcg cttgttctat acgccattat
     4021 agcacgaccg cagtaaaaac ccctctctga aatcagagag gggttttgga attatttgat
     4081 agatacaatt tctatcttgg tgtatttctg gcggtgacca gtttgcgtat tcacgcgctt
     4141 cttgctcttg tagcggataa cgcggatctt ttcgccggct accttttcgt ctacaacctt
     4201 agctttcacc tttacaccct ttacaaatgg cgcgccgaca gttatcttgt cgccatcgat
     4261 tagaaggagt gcgtcgagat cgagctcttt tgtgccttca gggaggaggt ccaccatgag
     4321 ggtctctttt tcggcgacaa tgtgttgttt gccacctact ttcacgactg ctttcatatg
     4381 tttccttatg atttaattgc gttcaagtaa gtattgtaac agatgtaagc agtttttaca
     4441 accagccaaa tacaacattc ttagcatggt catcaaagtc cttgatacat aatatagaaa
     4501 tcatggctct tgtaacattc ataccctaat atacgatatc taggactcgt cgtcttcggt
     4561 aaacgtcacc cagtccggat cgttatgaag atcatcttca acaccagacc aaaacttgct
     4621 taacgctgtc gtgtctggag atgggtcaag ctcccaagtc tcctcgtggt cagctttaat
     4681 ggcctgaata agctcgtcat tatcatcgtc aagtatcgca cttaattcga catgctgcgc
     4741 aggttgtgat tgaaagaatg agaatcgact tggtgataga tgtaacatat ttgtttgtgt
     4801 tagttgctaa ctatcaacta tttaagcaca aacattataa aatgtcaatt gtagagatga
     4861 gggtcattga gtgcccctat caagggacta actatctttt agaaagactt accctgagct
     4921 tcacgccctc gacagtaacc tcctcggtgt aatcgcctgc ctttgtgcca ccttcagcaa
     4981 gtgtctcagc atagatttct tcttgatgtt cgtcaatggc ctttgaaagc tctttatcat
     5041 cggtcactag aaacagctta atatggtcat cgacgttaag cccagctttc ttgcgcgcag
     5101 actgtacata ccggatcact tcacgcataa ggccttcacg cttcagtacc ggagtaagct
     5161 tctcatcaat cgccacgccc ccgccaagct tgacaatctt cacattaagt tcatctgtaa
     5221 gaattgcttt gtaatcgaca aatttgccaa gacttggcac cgcaacacta gctagtggct
     5281 gacgcacctt caatcccgcc tttgcacgaa ggctcagacc ttcattcaca tagccacgaa
     5341 cttgctccat ctcatcaagc acaagctgat caatgccaaa ctctgtaggc caatcaagca
     5401 aatgaaccga ctcgctatcg ccaccaaggt tctggtaaag ctcctcagcg aggaatggtg
     5461 taaacggtgc caaaataagt gaaagtttca aaagcacata gtggagcgta cgatacgcgt
     5521 cattcttatc gcttgtttct tcgctcttcc agaagcggcg acggctacgt cgcacgtacc
     5581 aattacttgc atcttcaata aacggcaaga tttcagccat cgcatcaggc aaattatacg
     5641 catccatatt atgcgtaata tgctcattta gttgatgcac acgtgagatc acccacttat
     5701 cgagcgggtt tgtcacctca gctattgaga gtgtttgcgc ttgcttagga tcaaattccc
     5761 aaccatcaac ctcagcgtac atcgtgaaga agtcatacat attccaaacc atactcagct
     5821 tgcgtgcaac atcaccaaca tccttgtcgt gcagtgcgaa gtcttcaccg ttgagtactg
     5881 ggctactcag taacaagaag cggagcgaat cagcgctaaa cttgtccatt agttcgttag
     5941 gatcagtaaa gttaccgtac gatttgctca tcttacggcc atcattgcca gcaagcgtac
     6001 cggtggtaat aacattttta aagctattcg taccaaacag cgcaacgctc attgcatgca
     6061 tgtaatagaa ccacgcgcga acctggccaa catattcaac aatgaagtca cctgggaaat
     6121 tcgcctcgaa tttctctttg ttctcaaatg gataatgaaa ctgcgcaaat ggcatcgagc
     6181 cggcttcaaa ccaactgtcc attactttat cgatacgagc gtactcttca ccatcgatcg
     6241 taaacttcac ttcatcaatc catggacggt gataatcctc aagttctatc ccactaagct
     6301 ctttaagttc agcatagcta ccgacaactt taatcttacc gctcttagtt ttccaaacag
     6361 gcaatggcgt cgcccagaaa cgatctcgac tcaagttcca atcaggtgcc tgctcgatgt
     6421 ttttcgcaaa acggccgttc tttaagtgtg ctggaaacca gtttacatta ccgttttgct
     6481 caagcatctg cgttttttgg tcagcaatgt tcgcaaacca gcttgggtgc gctcggtaca
     6541 taagacgcgt gccgcagcgg tgacagtgtg gataactgtg acgaatatat tcaatcttcc
     6601 aaacaatacc gcgctcttta agtgttttag cgattgcttt attttcttcc caaacgttcc
     6661 taccaagcca atcgccctct gtgtaaagac cgttttcatc aagcacatga acaacaggga
     6721 tcttcttttc gcgcgcaagt tcaaagtcct cttcaccgta cgcgggcgca agatgcacga
     6781 tacccgtacc gtcttcatcg ctgacatatt cagcgtccca taccttatgt gcattcttac
     6841 cgcgattaac gccaagaagt ggctcataac taagtccaac aagatcttta ccctttagct
     6901 cgcgtagcgc tgtatattca agtggttgat gcttttcgtt gacaaacacc tttgcaacaa
     6961 gatctttagc aacgactaat ttctcacccg cgactgcaac ttcaacatac gtaaagtctt
     7021 tatttatggc aactgccgta ttcgcaggta gcgtccaagg tgttgttgtc cacgcaagaa
     7081 gcgtcacacc attttcaagt ttaaatttca caaacacgct tgggtcagtt acatcctggt
     7141 atgcaccggc atccatggta acctcggcct ttgaaagcgg tgtaccgtcg agcgtgcagt
     7201 acataagtac cttctcgccc tcgtaaattt tacctttctc gtaaagctct ttaaacgccc
     7261 accaaatgga ctccatgtag tttttgtcca tggtcttata ggcaccgtta aaatcaaccc
     7321 atcgaccgat acggtcaatc acatcttccc aatcggcagc tgtttgcgtc atatacgcac
     7381 gcgttgcaat gatgtatttt tcaaggccat aatcaagcac atcttggtga ctactaagtc
     7441 cgagtttctt ctcactaaaa cgctcggctg gcaatccatg acaatcccaa ccccatacac
     7501 gctcaacacg cttacctttc atcgtccagt agcgtggtac ggcgtctttt acgatactac
     7561 taagaagtgt gccgtgatgt ggcacaccag aaataaatgg aggcccatca taaaacacat
     7621 atgcgtcgcc cgctggacgg ttctcaactg atttttcaaa cgtactctct agcttccagc
     7681 gctgcacccg atccttttcg tattcgagtg cgcgacgtcg tgtaccgtgt tgaaacttca
     7741 ttgcctatct ccttttctta tcatcattat ttctggaatc gtattatcaa acaatgtctc
     7801 ggaacctggt atttcctcaa atccccattt tcggtaaaat gccttcgcac gttcattgta
     7861 agtaactacc cctagctcaa tcggcttctt tgggttactc cagttgacaa tcttctgcat
     7921 gagagctcca ccaacacctt taccgtgccg attcttatcc gtatagagcg agccaacatg
     7981 ctggcgacct tcgtcatcta catatggcgt agccatgcca atcactcggc tatccaagtc
     8041 gaatgccacc cacccagcag tcttcgagct atcaagtcgc tcaagacgtc tagcgcgctt
     8101 ctcatccgac atctggtcga cgtttcgtgc ttctatccat tcccgggtca caccagcttc
     8161 atcattcaca taagtatcaa gccacaattg caagcgcata cgattgcttt cgtcaatcgt
     8221 agctgcagtc agttctgtaa tgataaattg tggctgctca gtcataatcc tctccaaaat
     8281 aaaaatccct tctcgctact ggaatccgat aaatcggacg gtaccatcca gtttcgaaaa
     8341 gggataactc c
//
LOCUS       DATIIX010000116        10232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_359710, whole genome shotgun sequence.
ACCESSION   DATIIX010000116 DATIIX010000000
VERSION     DATIIX010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 10232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10232
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359710"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="VLF64_03185"
     CDS             <1..1018
                     /locus_tag="VLF64_03185"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HSW90977.1"
                     /translation="KSKPAQNALNDLRGFGIVPDAVIVRSDDPAPDSVRSKVAMFGGV
                     SEDNVLMMPNVSSVFLIPERIAMSSIMPLLERFTNNTVQPDLSAWQDLAKRQATNFPH
                     NVTIGLVAKYLDNEDTYLSVLEALKSASWSEGVNLTIKWINAETATNEDFSAVDGLLV
                     PGGFGSRGIGGKIAAARFALETGKPYLGICLGLQVAVIAAARRAGILDANSSEFEVGA
                     THNVVYIMDGQQGKESTGGTLRLGNYPAHLVEGSLAERVYGVKEITERHRHRYEVNQA
                     YRDDIMKGGLTISGTSPDGRLVEFVEGTDGNFFIATQAHPEFRSRPMRAHPLFVGLIN
                     ASRR"
     gene            1075..1236
                     /locus_tag="VLF64_03190"
     CDS             1075..1236
                     /locus_tag="VLF64_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90978.1"
                     /translation="MAEEKPTPQNEPVNDTLTADDMDMLREVLQDAHSRGIAPVSVYK
                     QDDLAIDEF"
     gene            1278..2918
                     /gene="argS"
                     /locus_tag="VLF64_03195"
     CDS             1278..2918
                     /gene="argS"
                     /locus_tag="VLF64_03195"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HSW90979.1"
                     /translation="MEKTIAAIISIVQELYSIDLRVQLDRPDPKFGDYSTNVALQLAK
                     QVGKNPRDTATEIADKLKATGDYADVTVAGPGFINLTLGDKALLDLTRVNPGQIRQGQ
                     TVVIETNNPNPFKAMHIGHAYNAILADTMANLLAVSAATVRRVSYHGDVGTHVGKSMW
                     AILKETGGDIEKLEAIPVDERNIFMNKMYAIGAQAAKDSDEAKKEIDELAKQSFVLDD
                     PTYKAIYDMCAMWSYEQIDRIVARIGNVPIERRYVESETEKPGKELIVANTPGVFTQS
                     DGAYIFEGSKYGSFDNVYIGSHGNGLYGAHDMGLVQLKYADYPQLDQSIVVTGGEQAA
                     YFKGVIAASELAIPELKGKLFNYPTGLVKLTTGKMSSRTGDVVAIDWLFDEFGKAITE
                     RGGVAIDEIIAGALRYQFLKVKIGGDVVFDVNEAVSLTGNTGSYLQYAHARARRILDK
                     SAGTVSYPIDVRDEDRMLVRKLGEYHEVVNRALESLEPHHICTYLFELAQEFNRYYEQ
                     YRVIGDELETHRAGLVAVYADTLKAGLAILGIHAPERM"
     gene            2939..3361
                     /locus_tag="VLF64_03200"
     CDS             2939..3361
                     /locus_tag="VLF64_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HSW90980.1"
                     /translation="MTTKKQETIIRRDEDNKIERAARIRDVFSNQGAGFMDFIRQQGV
                     VGLAVGLAIGTAAGASVKAIVDNLINPLVALITQGVNLNNLKWVLEKANGSHPEAAIG
                     WGAILSSLVTLLATALVVYVAVHYAKLDRIDKKKEDAK"
     gene            3361..3906
                     /gene="orn"
                     /locus_tag="VLF64_03205"
     CDS             3361..3906
                     /gene="orn"
                     /locus_tag="VLF64_03205"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HSW90981.1"
                     /translation="MKKSFKPERILWVDLEMTGLDPVEDRILEIGAIVTDWDFKEIAR
                     FEAVKKVGPSLMKKRMVGEFWDEFSDVRDALIAQNMDGKNGRTVENELLAFIDEYIGG
                     EGKVLLAGNSIHQDRRFIANEWRRLDARLHYRMLDVSAWKVVFEGKFHKKFSKPEAHR
                     AIDDIRGSIEELEYYLKKVKA"
     gene            3903..4244
                     /locus_tag="VLF64_03210"
     CDS             3903..4244
                     /locus_tag="VLF64_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HSW90982.1"
                     /translation="MTHKDLEEYLLSFPGAWLDYPFGAETAVYKHGHKDVPGEDEKMF
                     AIIAEGSNPVRVSLKCDPLLAKHLREKYEEVQPGYHLNKKHWNTIICSGQLSDDEVKD
                     LARLSYRLVTE"
     gene            complement(4245..4895)
                     /locus_tag="VLF64_03215"
     CDS             complement(4245..4895)
                     /locus_tag="VLF64_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90983.1"
                     /translation="MPGYQPQTPFVPPSETNSQSPEYSIDYLNRIAPKEQRVVNRFAV
                     IGLIGGGILAAIFALILITASGGPSANDQLIPISERIATLQSVTKAEQTHLAEDQISE
                     ANAALSSSLSSMTTSMATILTERKLKDSTSSGAAKAEKLYAQNLTTTLEDSYQRGTLD
                     RTYTSQMTYELTVLKSRIAKLKQATGSKELLSFCDTSLNNIDLVLKAYSTFDATKS"
     gene            4868..5059
                     /locus_tag="VLF64_03220"
     CDS             4868..5059
                     /locus_tag="VLF64_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90984.1"
                     /translation="MEFVVGTQAWVWAATAAFGVVEAMETVAVASVEAAEVAAVGLVV
                     AKWSVVVVAKVAVDSSHQE"
     gene            5216..6970
                     /gene="dnaG"
                     /locus_tag="VLF64_03225"
     CDS             5216..6970
                     /gene="dnaG"
                     /locus_tag="VLF64_03225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HSW90985.1"
                     /translation="MQDAKEEVRSRLNIEDVIGEYVQLKRAGRNYKGLSPFSGEKTPS
                     FYVSPDKNIWHDFSSNKGGDVFTFVMEVEGMDFRQALEHLARKAGVELMEYESSDAKR
                     RAALKKRAIEANELAARYYQQNLLRNQHALEYVFKKRKLSKQVVQDFQIGYAPDNGSA
                     LVNFLLKRGFSKQEIAEAGLANRYGGDMFRGRMMVPLMDSSGQVIGFTGRIIGEVPNA
                     PKYLNTPQTLVYDKGRHIFALSQAKEAIRRAEYVVMVEGNLDVVSSHQVGVKQVVATA
                     GTAMTEHHLRAMKRLTGDVRLAYDSDKAGVAATERAIGLASQVGVELTVITLTDNAKD
                     PDELIQISPKKWLHVIDTAVPAVEWVLEQYAARLDLTTAAGKREFTTAGLNVVTTLND
                     PIEQEYYEQKIADISGSSLEAVRSKRASGSKPEPKLREVKAQRKQDLPGGEQYIYEDE
                     LLAVAAIDAPSQELFAHLDISYFHGEERQALARYYVTNGGKALDDTPEELQKYDTYVK
                     IVLLRADERYIKLDSNDRYYETARLIKQMKQEHMKQTRDTMTAELREAESNGDDARAM
                     QLLVQLNELTKEIARGKR"
     gene            6957..7943
                     /gene="rpoD"
                     /locus_tag="VLF64_03230"
     CDS             6957..7943
                     /gene="rpoD"
                     /locus_tag="VLF64_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HSW90986.1"
                     /translation="MANDDQIPTGDREEDDLMNIDPDEPAIDELMDEDEDTPEDILNS
                     GQYFDDISDDSVRLHLREIGKIPLLSADEELALAQRVVKGDKKAKDKMAEANMRLVVS
                     IAKRYSGRGLDFLDLIQEGHTGLLRAVEKFDPDKGFKFSTYATWWIRQAITRAIADQA
                     RTIRIPVHMVETINKLLRTQRRMTQELNREPTIEELAKELEMEPERVEYVIKIKQDIS
                     SLDAGVGRDGEDEDSVLGDFIEDEDGATPEESASSQLLKEQVQSVLSTLSEREQKIIK
                     MRFGLENGKSHTLEEVGQEFAVTRERIRQIEAKALAKLRKHKDAKKLYEYLQ"
     gene            complement(7991..8584)
                     /locus_tag="VLF64_03235"
     CDS             complement(7991..8584)
                     /locus_tag="VLF64_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90987.1"
                     /translation="MDQQSVLPPAPSVPARTPNPGQASGIIGVILAFVGLAPFGLILA
                     IISTVQSKKAKAPTTLGIIGIVINAIAVFVLAFLIIIISTHYSGIQETAKNADSKNAA
                     STVAKLAESYYAAHHSYPQSVSDFEKESGTSLAITRIHVTAIPPTDNSTIMYRACGLA
                     GAQIIYFSSVDNIPVSSYLGDGDAASCSANQPINKTA"
     gene            complement(8612..9190)
                     /locus_tag="VLF64_03240"
     CDS             complement(8612..9190)
                     /locus_tag="VLF64_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HSW90988.1"
                     /translation="MTEHRDTGTKILAIVGLTGSGKSEVVNYITEKGVPKVYFGGVIL
                     GAMKEAGIEWTAENEKQFREDIRAQEGKDFVVNRIVKEIRDLIDAGQRRIVADGLYTW
                     TEYKTLKHEFPGELTVAAVVAPKNLRHHRLTTRPIRPLTIEEANSRDWAEIENLEKGG
                     PIAIADHYLTNEGGLDTLHKQVDQLLDETHFL"
     gene            9273..>10232
                     /locus_tag="VLF64_03245"
     CDS             9273..>10232
                     /locus_tag="VLF64_03245"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HSW90989.1"
                     /translation="MSKPKRLVIIDGKSVFYRGYYAMPNLSTADGTPTGGVYGFAALS
                     IELIKKLKPDYVCVAWDKPKTNIRKRLEIYPEYKAGRKPAPADFYVQIPILHELLSAF
                     GWPLYELDDYEADDIMATLAHEAAKQGIEVCLITSDLDALQAIEPLVHVYALKKGLTN
                     IDLFKPESFTEKYGLRVDQFLDLKSLKGDSSDNIPGVPGIGEKTATQLLQQFETLDGI
                     YENLWQVKDSVRRKLEEGRDMAYMSKKVAELWFDAPIKLDLASMDCTLLDTAKLKELL
                     GQLEFKSLLRNLPDYMQDHSSVATQVSGSVTDVIELPASQAKAT"
BASE COUNT         2808 a         1998 c         2756 g         2670 t
ORIGIN      
        1 taaaagtaag cctgcacaaa atgcccttaa tgatcttcgt ggttttggta tcgtgccgga
       61 cgctgtgatt gtacgttctg atgatcctgc accagactct gtgcgcagca aggtagcaat
      121 gtttggcggc gtatctgaag ataatgtatt gatgatgcca aatgtctcaa gcgtattttt
      181 aattcctgaa cgtattgcga tgagtagtat tatgccgctt cttgaacgtt tcactaacaa
      241 caccgtgcag cctgatttat cagcatggca ggatctcgcg aagcgacagg cgacaaactt
      301 tccgcataat gtcacgattg gtcttgtggc gaagtatctc gataatgaag atacatactt
      361 atctgtgctt gaggccttaa aatctgcatc atggagtgaa ggtgttaacc ttactattaa
      421 gtggatcaat gcggagactg caacgaatga ggatttctca gccgttgatg gactgttggt
      481 gccgggtggc tttggttcgc gtggtattgg aggtaaaatt gctgcagcac gattcgcact
      541 tgaaacgggc aaaccgtatc taggtatttg tcttggacta caagtagcgg taattgcggc
      601 agctcggcgt gcaggaattc tagatgcaaa ttcatcagag tttgaggttg gtgctacgca
      661 taacgttgtg tatattatgg acggccagca aggtaaagag tcgactggtg gcacgcttcg
      721 ccttgggaat tatccagcac atcttgttga aggtagtctt gcggagaggg tgtacggcgt
      781 caaagagatc actgaacgcc atcgccatcg ctacgaagta aaccaggcat atcgtgacga
      841 tatcatgaaa ggtggtctca cgattagcgg tacgagccca gatggtagac ttgtagagtt
      901 tgttgaaggg acagatggta atttcttcat tgcaactcag gcgcaccctg aatttcgttc
      961 gcgcccaatg cgtgcacatc ccctgtttgt tgggcttatc aacgcaagcc gtcgctagat
     1021 atcttcggcc aggtttatgt tgacatttca tggtgtttat gctaatatca tttcatggca
     1081 gaagaaaaac caacaccaca aaatgagcca gtaaatgaca ctttaactgc agacgacatg
     1141 gacatgctcc gtgaagtgct acaggatgcg cattcacgcg gtatcgcgcc cgtgtccgtc
     1201 tacaaacaag acgatctcgc tattgatgaa ttttagctcg tttgccgaag gtatactacc
     1261 tctggtagaa tagcagtatg gaaaaaacaa tagctgctat tatttcgatt gttcaggaat
     1321 tgtattcgat tgatttgcga gtacagcttg accggccaga tccaaagttt ggtgattatt
     1381 cgacaaacgt agcattgcag cttgcaaaac aagtaggcaa gaacccgcgt gatactgcaa
     1441 ctgagatcgc tgataaactc aaagccaccg gtgactatgc tgatgtaact gtcgcaggac
     1501 cgggttttat taacttgacg cttggcgata aggcactcct tgatctgact cgcgttaacc
     1561 cagggcaaat ccgtcaaggt cagactgtgg tgattgaaac gaacaaccca aacccattta
     1621 aagctatgca tataggccat gcctacaatg caattttagc tgataccatg gcaaatcttt
     1681 tagcagtaag cgcagcaact gtacgccgcg ttagttatca tggtgatgtt ggcacacacg
     1741 tcggtaaaag tatgtgggca attcttaagg aaacgggcgg ggatatcgag aagcttgagg
     1801 ctattccggt tgatgagcgt aatatattta tgaataaaat gtacgcaatt ggtgcgcagg
     1861 cggcaaaaga ctcggatgag gctaagaagg aaatagatga gcttgcaaag caatcatttg
     1921 tgcttgatga ccctacgtat aaagcaattt atgatatgtg tgctatgtgg agctatgagc
     1981 agattgaccg tattgttgcg cgcataggta atgtgccaat tgagcgtcgt tatgtagaaa
     2041 gcgaaactga aaagccgggt aaggagctta tcgttgctaa taccccaggc gtgtttacgc
     2101 aatctgatgg cgcgtatatt tttgaaggta gtaaatatgg ctcgttcgat aatgtttata
     2161 tcggttcgca cggcaatggc ctatatggcg cgcatgatat gggtttagtg caacttaaat
     2221 atgccgatta tccgcagctt gaccagagta tcgtggtaac aggcggggaa caggcggctt
     2281 actttaaagg cgtgattgcg gcatctgagc tggctatccc cgaacttaag ggcaaacttt
     2341 ttaactatcc aactggcctt gtaaagctaa caactggtaa gatgagctca cgtactggcg
     2401 atgttgtcgc aattgactgg ttgtttgatg agtttggtaa ggcaatcact gaacgtggtg
     2461 gtgtggcgat tgatgaaatt atcgccggtg ctcttcgtta tcagttcttg aaagtaaaga
     2521 ttggtggcga tgttgtgttt gacgtgaatg aggcggtgag cttaactggc aacacaggca
     2581 gctatctgca atatgctcat gcacgcgcac gtcgtatttt agacaaatcc gcaggcaccg
     2641 tcagctatcc aattgatgtg cgtgatgaag atcggatgtt agtacgtaaa ctcggtgagt
     2701 accacgaagt tgtgaaccgc gcgcttgaat ccctagagcc gcatcatatc tgtacgtatt
     2761 tgtttgaact cgcacaggaa tttaaccgtt attacgagca gtaccgagtg attggcgacg
     2821 aacttgaaac gcatcgcgcg gggctggttg cagtatatgc agatacactt aaggcgggtc
     2881 tagctattct cggtattcat gcacctgaga gaatgtaacg agagagcgaa gcaataatat
     2941 gacaacgaaa aagcaagaaa cgataatccg tcgtgacgag gataacaaga tagagcgagc
     3001 tgcacggata cgagacgtat tcagtaacca aggagccggc tttatggact ttattaggca
     3061 gcaaggcgta gttggtctag cagttggttt agcaattggt actgcggcag gtgcgagcgt
     3121 taaggcgatt gtcgataatt taatcaatcc gcttgtagca cttattacac aaggagtgaa
     3181 tcttaacaac cttaagtggg tacttgaaaa ggcaaatgga tctcacccgg aagctgcaat
     3241 tggctggggt gcgattttga gttcgcttgt tacactgctc gcgacggcac ttgttgtcta
     3301 tgttgctgtt cattatgcaa aacttgatcg aatcgataaa aagaaagaag acgcaaagta
     3361 gtgaaaaaat cctttaaacc tgagcgaatc ttgtgggtag atcttgaaat gactggcctc
     3421 gatccggttg aagatcgtat cttggaaatc ggtgcaattg ttactgactg ggattttaaa
     3481 gaaattgctc ggtttgaggc agtgaaaaaa gtaggcccaa gtttaatgaa aaagcgtatg
     3541 gtgggcgagt tttgggacga attttctgat gtgcgcgatg cactgattgc acaaaatatg
     3601 gatggaaaaa acggtcgtac ggttgagaat gagctacttg catttattga tgaatatatt
     3661 ggcggagaag gtaaggtgct gcttgcaggc aattctattc accaagatcg acgctttatt
     3721 gctaacgagt ggcgacgact tgatgcacgg ttacactacc ggatgctcga tgtaagtgct
     3781 tggaaagtgg tgtttgaggg taagttccat aagaaattct cgaagcctga agcgcatcga
     3841 gctattgatg atatccgtgg aagcattgaa gagctagaat actatctgaa gaaagtaaaa
     3901 gcatgacaca taaagatcta gaagaatatt tgcttagttt tccaggtgca tggctcgatt
     3961 atccgtttgg agctgagacc gctgtatata aacatgggca taaggatgtg ccaggcgaag
     4021 atgaaaagat gtttgctatt attgctgaag gtagcaaccc ggtgcgcgtg agtctaaaat
     4081 gcgatccact acttgcgaaa catcttcggg agaagtacga agaagtgcag ccaggctatc
     4141 acttaaacaa gaaacactgg aatacgatta tctgtagtgg ccagctatct gacgacgagg
     4201 taaaagatct tgctcgtctt agctatcgtc ttgttactga gtaactacga ttttgttgcg
     4261 tcaaatgtcg agtaggcctt aagaacaagg tcgatattat tgagtgaagt gtcgcagaat
     4321 gaaagaagct cttttgagcc tgtagcttgc ttgagttttg cgatgcggga cttaagtacg
     4381 gtgagctcat aggtcatttg gctcgtgtag gtgcgatcga gggtgccccg ttggtaggaa
     4441 tcttcaaggg tagtagtaag gttttgagca tacaattttt cggcttttgc agcacctgag
     4501 ctggtgctat cttttagttt tcgctctgtc agaatagttg ccatgctcgt tgtcatgctt
     4561 gagagggaag atgaaagtgc ggcatttgcc tcggagattt gatcttctgc aagatgtgtt
     4621 tgctcggctt ttgtgacgct ttgaagtgtg gcgatacgct cggaaatagg aatgagttgg
     4681 tcattggcgc tcggaccacc tgatgctgtg ataagaatca gcgcaaaaat agccgcaaga
     4741 ataccaccgc cgataagtcc aatgacggca aaacggttta cgacacgttg ttcttttggc
     4801 gcaattcgat tgaggtagtc aatgctatat tcaggtgact ggctattagt ctccgatgga
     4861 gggacgaatg gagtttgtgg ttggtaccca ggcatgggtg tgggctgcga ctgctgcatt
     4921 tggggttgtt gaggccatgg agactgtggc tgtggcatct gtggaggctg ctgaggttgc
     4981 ggctgttgga ctggttgttg ccaagtggtc tgttgtggtt gttgcgaagg ttgcggttga
     5041 ttccagccac caggaatagg aggcacgcca ttgggcgaaa cccccggtgg gttcgagcca
     5101 ggttgtcctt gcggattcat agttatatta aaccacaagc aagtacccag aggaagagct
     5161 acccagaaaa gttccacact agtactaaag aggtgcagaa gttatactta gtactatgca
     5221 ggacgccaaa gaggaggtgc gtagtcggct caatattgaa gacgtaatag gcgaatatgt
     5281 tcagctgaaa cgagctggac gtaactataa aggtctgagc ccatttagcg gtgagaaaac
     5341 accgagtttt tacgtgagtc ctgataaaaa tatttggcat gactttagta gtaacaaagg
     5401 cggcgatgtc tttaccttcg tgatggaagt agaaggaatg gactttcggc aggcgcttga
     5461 gcacttagcg cgtaaggctg gtgtggaact catggagtat gaatcctctg atgcaaagcg
     5521 acgcgcggct ttaaaaaagc gtgcaattga ggcaaatgaa ctcgccgcac gttactatca
     5581 gcagaatctt ttacgtaacc agcacgcact tgagtatgta tttaagaaac gaaagctttc
     5641 aaagcaagtt gtgcaagatt tccagatcgg gtatgcgcca gataatggat cggcgttggt
     5701 gaattttctg ctaaagcgcg gatttagcaa gcaggagatt gctgaggcgg gacttgcaaa
     5761 ccgctatggc ggtgatatgt ttaggggtcg catgatggtg ccattaatgg attcgagcgg
     5821 acaggtgatc ggatttactg gccgcatcat tggtgaggtg ccgaacgcac caaaatacct
     5881 gaatactcca caaacgctgg tgtacgataa gggccgacat atttttgcgc ttagtcaggc
     5941 aaaagaagcg attcgccgcg cggaatatgt ggtaatggtg gaaggtaatc ttgatgttgt
     6001 gagcagccac caagtgggtg tcaaacaggt ggtggcgacg gcaggaacag cgatgacaga
     6061 acatcacctg cgagccatga agcggcttac gggcgatgtg cgattggcgt atgatagcga
     6121 taaagcaggc gtggcggcaa cagagcgagc aattgggctt gcatcgcagg ttggtgttga
     6181 gctaactgtc attaccctga cggataatgc aaaagatccc gatgaactga ttcaaatctc
     6241 accaaagaaa tggctgcatg tcatcgatac agcggtacct gctgtggagt gggtacttga
     6301 gcaatacgcg gcacgacttg acttgacgac agcggcaggg aagcgtgagt tcacgacggc
     6361 ggggctgaat gtggtaacga cattgaatga ccctattgaa caagaatatt atgaacaaaa
     6421 aattgccgat atttcaggga gctcccttga ggcagtacgc tcaaaacgag ctagtggatc
     6481 aaaacctgag ccaaagctac gcgaagtaaa agcacagcga aagcaagatc tgccaggggg
     6541 tgagcaatat atttacgaag atgaacttct tgcggtagcg gcaattgatg cgccaagcca
     6601 ggaactgttt gctcatcttg atatcagcta ttttcatggt gaagagcgac aagcattggc
     6661 gcgatactat gtgactaatg gcgggaaagc acttgacgat acgccagaag agttgcaaaa
     6721 gtacgatacc tatgtaaaaa tagtattatt acgtgctgat gaacggtata taaagctgga
     6781 tagcaacgat cgctattatg aaacagcccg cctcataaag cagatgaaac aagaacacat
     6841 gaaacaaact cgagacacaa tgactgcaga gctgcgcgaa gccgaatcta acggtgacga
     6901 cgctcgtgca atgcaactac tcgtccaact aaatgaacta acaaaggaga tagcgcgtgg
     6961 caaacgatga ccaaatccca accggcgacc gtgaagaaga cgatttgatg aatattgatc
     7021 cagatgaacc tgcgatcgat gaactgatgg atgaagatga ggatactcca gaggacattc
     7081 tgaactcagg ccagtacttt gatgatattt ctgatgactc cgtgcgccta caccttcgtg
     7141 aaattggtaa aatcccactt ctttcagcgg atgaagaact agcgcttgca cagcgagtgg
     7201 tgaagggtga taaaaaggct aaagacaaga tggctgaagc aaacatgcgt cttgttgtga
     7261 gcattgcaaa gcgctatagc ggccgtggcc ttgatttcct tgatttgatt caggaaggcc
     7321 acactggact cttgcgtgct gttgagaagt ttgatccaga taaggggttc aaatttagta
     7381 cgtatgcaac gtggtggatt cgccaggcga tcacccgtgc gattgctgat caggcgcgta
     7441 caattcgtat ccctgtacac atggttgaaa ctatcaataa gctactccgc acgcagcgcc
     7501 gcatgactca agagcttaac cgtgagccaa ctattgaaga gctcgcaaaa gagcttgaga
     7561 tggagccaga gcgtgttgaa tacgtgatta aaatcaagca ggatatttcg agccttgatg
     7621 ctggtgtggg tcgtgatggt gaagacgaag atagcgtgct tggtgacttt attgaagacg
     7681 aagatggtgc tacaccagaa gagtctgcaa gcagccaatt actgaaagag caggtgcaat
     7741 cagtactttc gacgctttca gagcgtgagc agaagatcat caagatgcgt tttggtcttg
     7801 agaatggcaa aagccataca ctcgaagagg taggtcaaga atttgctgtg acccgggagc
     7861 gtattcgcca gattgaagca aaggcgctcg cgaagctacg caaacacaag gatgctaaaa
     7921 agctttacga atacctgcaa tagagaatga tacatctaaa acgacgctat agagcgtcgt
     7981 tttttattgg ttaggcggtt ttgttaattg gctggttggc actacacgaa gctgcatcgc
     8041 catcgccaag atatgatgaa acgggaatgt tatcaacaga tgagaagtag atgatctgtg
     8101 caccggcaag cccacatgca cggtacataa tggtgctatt gtcggttggt ggtatggcag
     8161 tgacgtggat gcgagttata gcaaggcttg tgcctgattc tttttcgaaa tcgcttactg
     8221 actgcgggta ggaatgatgg gcggcataat agctttcagc aagttttgct acggtacttg
     8281 cggcattctt tgaatcggcg ttttttgctg tttcttgaat accggaataa tgagtgctaa
     8341 tgatgataat aagaaaggcg agcacgaata cggcgattgc attaataaca attccaataa
     8401 ttccgagcgt tgtcggggct tttgcttttt tagattgaac ggtcgaaata atagcaagga
     8461 tcagcccaaa cggtgcaagg cctacaaatg cgaggatgac gccgatgatg ccggatgctt
     8521 ggcctggatt gggtgttcga gcagggacgc ttggcgcagg aggaagtact gattgttgat
     8581 ccatactact tatagttata gcacgtggcg cctataaaaa gtgggtttcg tcgagtaatt
     8641 gatcaacttg tttatgcaat gtgtcgagac caccttcgtt agtaaggtag tgatcggcga
     8701 tagcaatagg gccgcccttc tcgagatttt cgatttctgc ccagtcacgt gaattggctt
     8761 cctctatggt cagagggcga atagggcggg tggtaaggcg atggtgccgc aggttttttg
     8821 gtgcaacgac agcggcaaca gtgagctcgc cgggaaactc atgcttgagg gttttgtatt
     8881 cagtccaggt gtagagtccg tcggccacaa tgcggcgctg accagcatca ataagatcac
     8941 gaatttcttt tacgatacga ttaacaacaa agtctttgcc ttcttgggca cgaatatctt
     9001 cgcggaattg cttttcgttt tctgcagtcc actcaatgcc cgcttctttc atggcgccga
     9061 gaattacgcc gccaaaatat actttgggga cgcctttttc ggtgatgtaa tttactactt
     9121 cagatttgcc gctgcctgta aggccaacga ttgcaagaat ttttgttccg gtatcgcggt
     9181 gttcagtcat agcactagtg taacagagag ggaaaccact tgtatatatg tgtatggctt
     9241 tttgctcacc accccgatgg tatactggta ctatgagcaa accaaaacgt cttgtaatta
     9301 tcgatggtaa gtcagttttc tatcgtggct actatgcaat gccaaacctt agtactgccg
     9361 atggtacacc aactggtggt gtatatgggt ttgcagcgct tagtattgag cttattaaaa
     9421 aattgaagcc agattatgtt tgtgtcgcgt gggataagcc aaagactaac attcgcaagc
     9481 gtcttgagat ttaccctgaa tacaaggcgg gacgtaagcc ggcgccggca gatttttatg
     9541 tgcaaattcc gattctacat gaacttcttt cagcatttgg ttggccgcta tatgaacttg
     9601 acgattatga ggcggatgac attatggcaa cgcttgcaca tgaagcagca aagcagggaa
     9661 tcgaagtctg tctcattacg agcgaccttg acgcattgca ggcgattgag cctctcgtgc
     9721 acgtatacgc gcttaaaaaa ggtcttacta atattgattt gtttaaaccg gagagcttta
     9781 cggagaagta tggtttacgg gttgatcaat ttctcgatct taaaagtttg aagggtgatt
     9841 cgagtgataa tattccgggc gtaccaggaa ttggtgaaaa gactgcaaca cagcttttgc
     9901 aacaatttga aacgctcgat ggtatctatg aaaatctttg gcaagtgaaa gatagtgttc
     9961 gtcgcaagct ggaggaaggc agggatatgg cgtacatgag caagaaggtg gctgaactat
    10021 ggtttgatgc gccaattaag ctggatttag cgtcgatgga ttgcacactt cttgatactg
    10081 caaaactgaa agagttactt ggccagcttg agtttaagag cttactgcgc aacctaccag
    10141 actacatgca agatcattcg tcagtggcca cacaggtatc gggtagtgtt acggatgtga
    10201 ttgagttacc cgcgagccag gctaaagcaa cg
//
LOCUS       DATIIX010000117         6957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_471479, whole genome shotgun sequence.
ACCESSION   DATIIX010000117 DATIIX010000000
VERSION     DATIIX010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6957
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471479"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VLF64_03250"
     CDS             <1..393
                     /locus_tag="VLF64_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90990.1"
                     /translation="RLIIAQRLVRRLVDETKEEYEPDEATRNYVKRVLEGIPEDTDHP
                     DLDSFKLWRPKPSEDAPFGYKGRIVIMEQLIVDEEIQKFIRGDIAEVHPEAIEKTARE
                     RGMLTLEQVGVLAALRGETTLDEVGRVI"
     gene            complement(516..926)
                     /locus_tag="VLF64_03255"
     CDS             complement(516..926)
                     /locus_tag="VLF64_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90991.1"
                     /translation="MPLLTEDFCTDVRQNGEWVGTLRVHVQFDLEDPFALTFMLLQAE
                     SYHQQTLAVKLFRRAFKRPGKPQGKGAIKVIYMELEGVVSVEFPNSELGPFQVLFDEV
                     ALSQYLTCIGQLVQFNQLKKQYLRYGIAQLLDQE"
     gene            complement(1097..1513)
                     /locus_tag="VLF64_03260"
     CDS             complement(1097..1513)
                     /locus_tag="VLF64_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW90992.1"
                     /translation="MSVLPVLEFSAAIINTPTGRHDTTVVLSYVSERPDVFRLSISFS
                     ADEAASRVVHVHALRNALANPGELIDGCIMAVPDCETVIIYLSAGNGEYAMLYLDMQT
                     AQSFVDTIDVEMIAEQAVDTVTHQGFEDLCKWLAYQ"
     gene            complement(1615..2190)
                     /locus_tag="VLF64_03265"
     CDS             complement(1615..2190)
                     /locus_tag="VLF64_03265"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HSW90993.1"
                     /translation="MKARTHDLAALTALGIVVISQPVNTISLGTMLVALLANQIGGIA
                     PDIDQPTAPLWRNLPIGGVVGRIVDKMLGGHRFVTHSLLGLGLFGVGLWWMLALLQPI
                     VPHIDWHLVWWAFMIGMASHLVMDTFTKEGVPWLLPIPIKFGFPPTKSLRITTGKAGE
                     FVIFCLILLLDMWYCASHYNAILSLIHERII"
     gene            complement(2217..3350)
                     /locus_tag="VLF64_03270"
     CDS             complement(2217..3350)
                     /locus_tag="VLF64_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HSW90994.1"
                     /translation="MDYDKLALDLHKQFKGKITTKLRDESELDRTRLSAYYTPGVAAV
                     SKAIADDPSLLPTYTWTNNLVAVVSDGSAVLGLGNIGPEGSMPVMEGKALLFKHFAGL
                     DAVPITLNVHTADEIVATVKAISPSFGAINLEDIAAPLCFEIEERLKAELSIPVFHDD
                     QHGTAVVTLAGLINSMRITGKSLKDCKVVTIGAGAAGTAIMKLLHAYGVGSIIAVDSH
                     GIINDMRDDLNDEKKALLPYLDISLSGSLKDAVQGADIFIGVSKPGLLTEDMVRTMAK
                     DPIIFALSNPTPEIMPDYAKAAGAAIIATGRSDFPNQINNAIAFPGIFRGALDNGVQK
                     ITDEHKIAAAEAIAALIDAPTTDEIIPSVFDERLVPAVAAVIR"
     gene            3529..3876
                     /gene="rplS"
                     /locus_tag="VLF64_03275"
                     /pseudo
     CDS             3529..3876
                     /gene="rplS"
                     /locus_tag="VLF64_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            4136..4756
                     /locus_tag="VLF64_03280"
     CDS             4136..4756
                     /locus_tag="VLF64_03280"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HSW90995.1"
                     /translation="MILGIDEAGRGPWAGPLVVGAVVLGGEIDGLNDSKKLTKRHREK
                     LESEILERAQATATGWVSATELDEIGMSAALRLATRRAVEQITVPYHEIIIDGTVNFL
                     RDTSKNGYVTTLPKADALIPSVSAASIIAKVARDRYMTSLGEKYPEYGFKNNAGYGVI
                     KHRDAIERIGVMDEHRLSFAPLQKYRATPPTSMTSLHTARPQGDNA"
     gene            4762..5088
                     /locus_tag="VLF64_03285"
     CDS             4762..5088
                     /locus_tag="VLF64_03285"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HSW90996.1"
                     /translation="MTRAKGWPPKNLFGSVIRLLIVTGKQKYCEIDIISPNGDTIYFT
                     EVKHRKNTEHGDGLEVIIPRKRRQMTFAARMYAQAHKLTNVNLQLLAIATTGNNPVVE
                     LVIEIV"
     gene            complement(5068..>6957)
                     /locus_tag="VLF64_03290"
     CDS             complement(5068..>6957)
                     /locus_tag="VLF64_03290"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HSW90997.1"
                     /translation="NIWASPAQHANTLDFALENATKIIDFYEEYFGVDYPLAKCDHVA
                     LPDFSSGAMENWGLITYREVCLLADPKTTGPSGRHLIASVIAHELAHQWFGNLVTMEW
                     WDYLWLNESFADFVEHIAIDVIHPEWETWLDFILSRGIAALRRDAIEGVQSVQVMVKH
                     PDEISTLFDGAIVYGKGARLMKMLRAFVGEAAFRAGLKEYFTMYAYSNTTGDDLWACL
                     SKASSKDVGAFMNTWITQPGYPVVIVKPEGLSQTQFFIGAHGPSAQTWPVLLGATPVG
                     DTPEIMYDGELAATIKPTQRFNSDDNGHFITQYTPEHLNTLLSDIHSASTIDRLTLLN
                     NQTLLVRGRYEPSASLIDLLNFYEGETNDAVWDIMAMTLGELKKFVETDNAAEQKLRA
                     LSARIARSNFERLGFEPIKGESITDTKLRETVLGMMLYGEDATVIDGMMSRFDIAMLA
                     ELPAEIRSLVIGTVVRYTDDNTVIDQLMDTYQSTPSAELKEDIMAAMTSTRDPEVATR
                     LLADCKNAKIVRPQDVAPWFVYLVRNRFTRTVAWKWLQDNWNWVVATYSGDMSFDEFP
                     RYAASGLMTNEQLVEYKRFFEPMLDNPSLTRTITMGISEIAARLELLASDGKAVRVKL
                     HDLDN"
BASE COUNT         1892 a         1697 c         1729 g         1639 t
ORIGIN      
        1 cgcctaatta ttgcacagcg cctagtacgt cgcttggttg atgaaaccaa agaagagtat
       61 gagcctgatg aagcaacccg caactatgta aagcgtgtgc ttgaagggat acctgaagat
      121 accgaccatc cagatctcga cagcttcaaa ctttggcggc caaagccaag tgaagatgcg
      181 ccgtttggct ataaaggccg cattgtgatc atggaacaac taattgttga cgaggaaatc
      241 caaaagttca tccgcggcga tatcgccgaa gtccacccag aagcaatcga aaagaccgcg
      301 cgcgaacgcg gcatgctcac actcgaacaa gttggtgtac tcgcagctct tcgcggtgaa
      361 acaactcttg atgaagtcgg acgcgttatc tagtcgtatg gcgctataag caacacccgc
      421 cttttctgag gcgggtgttt tgtatcttgg aggatccccg gcgccactca tacatgaggg
      481 gtcgccgagg gcccttgtcg cggtggagta ggttactact cctgatcgag aagctgtgcg
      541 atgccatacc gaaggtattg cttcttgagc tggttgaatt gaaccagctg gccgatgcag
      601 gtcaggtact gggagagggc gacttcatcg aagagaacct ggaacggccc cagctcgcta
      661 ttcgggaact caacgctgac aaccccctcc agctccatgt agatgacttt gatggcgccc
      721 ttaccctgcg gctttccggg gcgcttaaat gcgcgcctga acagcttgac ggcgagagtc
      781 tgctggtgat agctctcagc ctgaagcaac atgaaggtga gcgcgaaggg gtcctcgagg
      841 tcgaattgca cgtgaactcg aagggttccg acccactcgc cgttctgtcg cacgtcggtg
      901 cagaaatctt ccgtaagcag aggcatcgta ggtgctcctt tcgtgcaata tgcgctgaca
      961 aggttgtgct atagtaccgt gcagcgtaac agttgtcaat gacaaataaa aaagacgcgt
     1021 atcgcgtctc tagtgggcaa ccttcggcca ctccctaaag cgggagcggc cgaagtacct
     1081 ttcagcctag ctcgccttat tgataggcga gccacttgca gaggtcttca aacccctggt
     1141 gcgtaaccgt gtcgacggcc tgctcggcga tcatctcgac gtctatggtg tccacgaagg
     1201 actgcgcggt ctgcatgtcg aggtacagca tggcgtactc gccattgccc gcggacaggt
     1261 agatgatgac cgtctcgcaa tcggggactg ccataatgca gccatcgatc agttcgccgg
     1321 ggttcgctag cgcattgcgc agcgcgtgaa catgcacgac gcgcgatgcg gcctcatcag
     1381 cactgaagct gatgcttagc ctgaacacat ccggtcgttc cgacacgtag ctcagaacaa
     1441 cggtagtatc gtgccgcccc gtaggggtgt tgatgatggc agcactgaac tccagaacgg
     1501 gcagaacaga caacgtaggt actccttgca gggataaagc tgataggaca acacacatta
     1561 tatactactt atgctatcct gtcaatgcat atgtcatata taccacggcc tgatctatat
     1621 aatccgctcg tgaataagcg agagaatagc attgtagtgc gaggcacagt accacatatc
     1681 gagtaggaga atcaaacaga atataacgaa ttcacccgct tttccagtgg tgatacggag
     1741 agactttgta ggtggaaagc caaacttgat tggtataggc aacaaccaag gtacaccttc
     1801 tttggtgaat gtgtccataa caagatgcga agccatgccg atcatgaatg cccaccatac
     1861 aagatgccaa tcaatatgtg gcacaattgg ttgcagaagg gcgagcatcc accatagacc
     1921 aacgccaaac agaccaagtc caagaagcga atgggttacg aagcgatgac cgccaagcat
     1981 cttatcaaca atgcgtccga caaccccacc tatcgggagg ttgcgccata gtggtgccgt
     2041 cggttgatcg atatcaggtg cgataccgcc aatttgattc gcgagcaggg caacgagcat
     2101 ggtaccgagt gatattgtat ttaccggctg agatataaca acgataccga gcgcagtaag
     2161 tgcagcgaga tcgtgggtgc gtgctttcat agcactagtg tagcatgagc gggttcctac
     2221 ctaataacag cagcaacggc aggcactagt cgctcgtcaa acacactcgg gatgatttca
     2281 tcagtcgttg gcgcatcaat aagtgcggca attgcttctg cagctgcaat tttatgttca
     2341 tcggtaattt tttgtacccc gttatcaagt gcaccacgaa agataccagg aaaggcaata
     2401 gcattgttga tttggtttgg aaagtcgctg cgaccagtcg cgataatcgc cgcaccagca
     2461 gcttttgcgt aatctggcat aatttcgggg gtcggattag aaagcgcgaa gataatagga
     2521 tcctttgcca tcgtgcgcac catatcctca gtgagcaagc ctggttttga tacaccaatg
     2581 aagatatctg caccctgaac cgcgtcttta agcgaccctg acaatgaaat gtcaaggtaa
     2641 ggaagcaatg cttttttctc gtcattcaaa tcatcacgca tgtcgttgat aatgccatga
     2701 gagtcaacag caataattga cccaacaccg tatgcatgaa ggagcttcat aatagctgta
     2761 ccagccgccc cagcacctat ggtgacaact ttgcaatctt taagactttt accggtaatg
     2821 cgcattgaat taatgagccc tgcaagcgtt acgacagcgg taccatgttg gtcatcatgg
     2881 aatacaggga tgctcagctc agccttaagg cgctcttcaa tctcaaaaca gagtggtgca
     2941 gcaatgtctt caaggttgat tgccccaaag ctgggcgaaa tcgcctttac tgtagcgacg
     3001 atttcatccg ccgtgtgcac attcaatgta attggcacag cgtcaagtcc agcaaagtgc
     3061 ttaaagagta atgctttacc ctccatcaca ggcatgctgc cctcaggtcc aatattgcca
     3121 aggcctagga cggccgagcc atcgctcacc acggccacaa gattatttgt ccaggtgtac
     3181 gtcggtagaa ggctaggatc gtcggcgata gccttactga cagctgctac gcctggtgtg
     3241 tagtaggcac ttagtcttgt acggtcaagt tcactctcat cgcgcagttt cgtcgtgatt
     3301 ttacctttaa attgtttgtg taaatcaagt gcaagtttgt cataatccat aactctagta
     3361 tacgcctgcg tgattttacg gttagcccct ttcaaaatca tgaaaactat gctatagttc
     3421 atcattgatt gtatgtagta accgccgatg attcgtggtg tggagccgta gccgtacaac
     3481 tacgtggcca gccgacttga actaagcatt aaccagagga acaatttaat gagttttgca
     3541 cttatccaaa aagtcaacga tgagcagaaa aaggcacaag ttgttgacgt tcgcagcggc
     3601 gacaccgtcc gcgttcacca gaagattaaa gaaggtaaca aagagcgtat tcagatcttt
     3661 gaaggcgttg ttatccgtac tgacaacaaa ggccagcaca caagtcgtat cactgtccgc
     3721 aaaatcgcta gtggcgttgg cgttgaaaag agcttcttgc tccacagccc acttgttgaa
     3781 aaggtagaaa ttgtacgtcg tgctaaggtt cgccgtaact tccttagctt ccttcgcaat
     3841 cgtagtggta agagcgctcg tcttgcagcc gtacagttcg accgcgaagc agtgaacgct
     3901 gtcgttgata agcacgctga agaagaggaa gctcgtctta aagaagaagt taaggctgaa
     3961 gctgaaacta aggctgccga aaaagccgct gagctagctg aactcgaagc aaagcaaaaa
     4021 gctgttgaag acgcacacaa agctaacagc taatcactcg cagaaatata tacaaaactc
     4081 ccctgcttct aggggagttt tgtataggcc tgcagaagta gcgtacactg gtaatatgat
     4141 cttggggatt gatgaagcag gccgtggacc ttgggcgggg ccgctggtgg tcggtgcagt
     4201 cgtacttggt ggcgaaatcg atggtcttaa cgacagtaaa aaacttacaa agcgacaccg
     4261 tgaaaagctc gaaagtgaaa ttctcgaaag agcgcaggcc acagctactg gatgggtaag
     4321 tgcgaccgag ctcgacgaga taggcatgag tgccgcgctg cggcttgcaa ctcgccgagc
     4381 agtagagcaa atcacagtgc cctatcacga aattatcatt gacggtacag tgaattttct
     4441 gcgtgataca tcaaagaacg gttatgtgac gacactgcca aaagccgacg cactcatacc
     4501 aagcgtaagc gcagcctcta ttattgcaaa ggttgctcgc gacaggtata tgacaagcct
     4561 tggagaaaaa tacccagagt atggctttaa aaataatgct ggatatggag tgataaagca
     4621 ccgcgatgca atcgagcgga ttggagttat ggacgagcat cggctgagct ttgcaccact
     4681 acagaagtac cgtgctacgc cgccaaccag catgacctca cttcataccg cccgcccaca
     4741 aggtgacaac gcgtagtatc ggtgacacga gcgaaagggt ggccgccaaa gaacttattc
     4801 ggctcggtca tacgattatt gatcgtaact ggaaaacaaa aatactgcga aattgatatt
     4861 atttcgccaa atggcgacac gatatacttc accgaagtaa aacaccgcaa aaacactgag
     4921 cacggtgatg gattagaggt tattatccca cgcaagcggc gacaaatgac atttgccgcc
     4981 cgaatgtacg cgcaggctca taaacttacg aatgtaaacc ttcagctact cgcgattgcc
     5041 actactggca ataatccagt ggtagaacta gttatcgaga tcgtgtaatt ttacgcgaac
     5101 cgctttgcca tcactagcaa gaagctccag gcgcgcggca atttcagaaa tacccatagt
     5161 aatagtgcgc gtgagagatg ggttatcgag cattggctca aagaatctct tatactcaac
     5221 aagctgttcg ttagtcatta gtccactcgc tgcataacga ggaaattcat caaaactcat
     5281 gtcgccactg taagttgcaa ccacccaatt ccagttgtct tggagccatt tccaagcaac
     5341 agtacgagta aagcgattac gaacaagata cacaaaccat ggtgcgacat cttgcgggcg
     5401 gacaatcttg gcattcttgc agtcagcaag caggcgtgta gctacttcgg ggtcacgtgt
     5461 tgaggtcatt gcagccatga tgtcttcttt aagctctgct gatggcgtac tttgatatgt
     5521 atccatgagt tgatcgatga ctgtgttgtc atcggtatag cgcacgaccg tcccaataac
     5581 aagcgagcgg atttccgctg gaagttcagc gagcattgcg atatcaaaac gagacatcat
     5641 accatcaatt actgtcgcat cttcaccata aagcatcatt ccgaggacgg tttcgcgcag
     5701 tttagtatcg gtgatggact cacccttgat tggctcgaag ccaagtcgtt caaaattact
     5761 gcgtgcgata cgcgccgaaa gggcgcgaag tttttgttcg gcagcattgt ctgtttcaac
     5821 gaacttctta agttcaccga gcgtcatcgc catgatatcc cagacggcgt cgtttgtctc
     5881 gccttcgtaa aaattcagca aatcaatgag tgatgcagac ggttcgtagc gtccacgcac
     5941 gagaagcgtc tgattattaa gcaaagtcag ccgatcaatg gttgatgcgc tatgaatgtc
     6001 agaaagcaaa gtgttgaggt gttccggggt gtattgggta atgaagtggc cgttatcgtc
     6061 actgttaaat cgttgagtag gtttaatagt agccgcaagt tctccgtcgt acataatttc
     6121 tggtgtatcg ccaacaggcg ttgccccgag aaggactggc caggtctgtg cgcttggccc
     6181 atgtgctcca ataaagaatt gagtctggct aagcccttct ggcttaacga tcacaacagg
     6241 gtagcctggc tgagtaatcc aagtattcat aaaagcaccc acatctttac tggatgcttt
     6301 actgagacat gcccacaggt catcacctgt tgtgttgcta taggcataca tagtgaaata
     6361 ctctttaagg ccagctcgaa acgctgcttc accaacaaac gcccggagca tcttcataag
     6421 tcttgcgcct ttaccataca cgatagctcc gtcaaagagc gtcgaaatct catctgggtg
     6481 tttgaccata acctgtacag actgcacgcc ttctattgca tcacggcgca gtgctgcaat
     6541 gcctcgtgac aaaataaagt caagccatgt ttcccattct gggtgaatca catcaatagc
     6601 aatgtgctcg acaaagtcgg caaaactctc attcagccag aggtaatccc accactccat
     6661 agtaaccaga ttgccaaacc actggtgagc aagttcatgt gcgatcacac tggcgatgag
     6721 gtgcctgcca ctcggaccag ttgttttagg gtcggcaagc aaacaaactt cacggtaggt
     6781 aataagtccc cagttttcca tcgcaccact actgaaatct ggtagagcga catgatcaca
     6841 cttagcaagc gggtagtcga caccaaaata ctcttcgtaa aagtcaataa tctttgtggc
     6901 attttcaagt gcaaaatcaa gcgtgttggc atgctgtgca ggacttgccc agatatt
//
LOCUS       DATIIX010000118         2958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_360257, whole genome shotgun sequence.
ACCESSION   DATIIX010000118 DATIIX010000000
VERSION     DATIIX010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360257"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /gene="priA"
                     /locus_tag="VLF64_03295"
     CDS             <1..782
                     /gene="priA"
                     /locus_tag="VLF64_03295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HSW90998.1"
                     /translation="LRCHICAYTEKVPTSCPACHETDIIHKGIGTKLVESEIRRLFPN
                     KNIMRFDGDTEATETIEHQYQALYSGEVKIIIGTQVVAKGLDLPKLSTVGVIQADAGL
                     SLPDYSSSERTFQLLAQVIGRVGRSSRKANVIIQSYQPTNPAVQYGIEQNYEMFYQET
                     LALRKRSNFPPFTYLLKLTCTYKTEAAAIRNSKALADLIRSRYPNISVLGPTPAFYER
                     QRDTYRWQITIKSTKRAQLLAIIGEVPGQYWQYDIDPMNLL"
     gene            827..1402
                     /gene="def"
                     /locus_tag="VLF64_03300"
     CDS             827..1402
                     /gene="def"
                     /locus_tag="VLF64_03300"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HSW90999.1"
                     /translation="MKKESIIILPNPHLRQKSTRIHVVTDDIRKIIDNMTSASLDWED
                     SRPHEISAALAAVQIDKLERIVIVRSDFDDKAVREFIPLINPEIVKGEGPMITDYEGC
                     LSVDGFYGKVPRYSKVRIKAMDINGNEVRIKAEGFLARVLQHEIDHTNGIVFVDHIRD
                     QHDAFYTLNAKGELDPLDYDTHVKDNPKLWD"
     gene            1371..2276
                     /locus_tag="VLF64_03305"
     CDS             1371..2276
                     /locus_tag="VLF64_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HSW91000.1"
                     /translation="MSKTTLSSGINQPIVFFGTEEHSLIALRGLVDAGLPIAAVVTKP
                     DAAKGRGKKLTPPAVKVYADAHSIAVWQPTRLKEITEDIKKLDNPIGVLVSYGKIIPK
                     KTLEVFTPGIINVHPWLLPKYRGPSPIESAIVNRDNKTGVTIMRLISAMDAGPIYQQV
                     PYVLDFTETKPELYNTLFTLGTHLLISKLPAIVSGELQPMPQDDNNATYCALLSKEDG
                     DLDLTTLTPGEAEARVRGYLGYPRTRVTIGNHTIIITKAHGVMTQKTPLDLMCANGAF
                     LSIDELIAPSGKTISAAEFLRGYSL"
     gene            complement(2273..2695)
                     /locus_tag="VLF64_03310"
     CDS             complement(2273..2695)
                     /locus_tag="VLF64_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5663 domain-containing protein"
                     /protein_id="HSW91001.1"
                     /translation="MFQLDDNFLQEVGLGDLPEDQKKLFLGHFREQLELRVGTQLSEG
                     LSDAQLQEFESFLDRKDEVINAWLAANAPAYEQDSIYQQLTSSAPENVPRNAVLAEYA
                     SLKWLSLNRPDYKDVVSRTMNELKGEIITNRDAILGTV"
     gene            complement(2730..2885)
                     /locus_tag="VLF64_03315"
     CDS             complement(2730..2885)
                     /locus_tag="VLF64_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW91002.1"
                     /translation="MQQASEIAEQLPEDGRLDAYLDLIYEIDARLGDEQASGSDTSAQ
                     GGANPTA"
BASE COUNT          850 a          783 c          602 g          723 t
ORIGIN      
        1 aactccgttg tcatatatgc gcatatactg aaaaagtacc gacatcatgt ccagcctgcc
       61 acgaaaccga catcatccat aaaggcatcg gcacaaaact tgttgagtct gagattcgtc
      121 gtctcttccc gaacaaaaat attatgcgct tcgacggtga cactgaagcc acagagacaa
      181 tagagcatca ataccaggcg ctctacagtg gcgaagttaa gattattatt ggtactcagg
      241 ttgtagccaa aggccttgac ctaccaaaac ttagtaccgt tggcgtcatt caagcagacg
      301 ccggactaag ccttcccgac tactcatcaa gtgaacgaac atttcagtta ctcgctcagg
      361 tcattggccg agttggcaga tcttcgcgca aggcaaacgt cattatccaa agttaccaac
      421 caactaatcc ggccgtccaa tacggcatcg agcaaaacta cgaaatgttc taccaggaaa
      481 ctctcgcatt gcgtaaacga agtaacttcc caccatttac ctacctgctg aaactaacct
      541 gcacctacaa aactgaagct gccgcaattc gtaatagcaa agcactcgca gatcttatac
      601 gcagtcgata tcccaatata tcagttcttg gccccacccc cgccttttac gaacgacaac
      661 gcgatacata tcgttggcaa ataaccatca agtcgacaaa gcgggcacag ctgctggcaa
      721 ttatcggtga agttcccggg caatattggc aatacgatat agatccaatg aatctactct
      781 aatctacctc tgttcttatg ttgctttttc atatataatt agagcaatga aaaaagaatc
      841 tatcatcatc ctgcctaacc cgcatctacg gcaaaaatca acacgaatac acgtagttac
      901 agatgacatt cgtaaaatca tcgacaacat gactagtgcg agtcttgact gggaggactc
      961 gcgccctcac gaaatcagcg ctgcgctcgc agctgtgcaa attgacaagc tcgaacgcat
     1021 cgttattgtt cgtagcgact tcgacgataa ggccgtccgc gaatttatcc cgcttattaa
     1081 tcccgagatc gtaaagggtg aaggcccaat gattacagat tatgaaggct gccttagcgt
     1141 cgacggtttc tatggcaaag tgccccgcta tagcaaagta cgcatcaaag ctatggatat
     1201 aaatggcaat gaagtgcgca ttaaagctga aggttttctc gcgcgtgtat tacagcatga
     1261 aattgaccac acaaacggca tcgtatttgt tgaccacatt cgcgatcagc acgacgcatt
     1321 ctacacactt aacgcgaaag gcgagctgga tccgctcgat tacgacacac atgtcaaaga
     1381 caaccctaag ctctgggatt aaccagccaa ttgtcttttt tggtaccgaa gaacacagcc
     1441 taattgcact tcgcggactt gtcgacgcgg gtctacctat tgccgctgtt gttacgaaac
     1501 ccgatgccgc aaaaggccgt ggtaaaaaac ttactccacc agcggtaaaa gtatacgccg
     1561 atgcgcatag tatcgctgtc tggcagccaa cccgcctcaa agaaattacc gaagacatca
     1621 aaaaactcga taaccctatt ggtgttcttg taagttacgg caaaattatc ccaaaaaaga
     1681 cacttgaggt tttcacccct ggcatcatca atgtacatcc gtggctacta ccaaagtacc
     1741 gcggaccatc cccgattgaa tcagcaatcg ttaaccgcga caataaaaca ggtgtaacga
     1801 ttatgcgcct tataagcgcg atggacgctg gaccgattta ccagcaagtc ccctatgtac
     1861 ttgatttcac cgaaaccaag ccggagttat acaacaccct atttacgctt ggtacccatt
     1921 tacttattag caagcttcct gctattgtat ccggcgagct gcagcccatg ccacaggacg
     1981 acaataatgc aacatactgc gcacttcttt ctaaggaaga tggtgacctt gatcttacaa
     2041 cactcacccc cggtgaagca gaggctcgcg taaggggcta cctcggctat ccacggacac
     2101 gcgtcacaat tggtaatcat actattatca ttacaaaagc ccatggtgta atgacacaaa
     2161 aaacaccgct cgaccttatg tgtgcgaacg gtgcattttt gtctattgat gaacttattg
     2221 cgccaagtgg caaaacaata agcgctgccg aattcctgcg cggctattcc ctctagaccg
     2281 tgccaagaat tgcatcacga ttagtgataa tctcgccttt caattcattc atagtgcgcg
     2341 acacaacatc tttatagtcg gggcggttga gactcaacca cttcaaagat gcatattccg
     2401 ctagcactgc attccgtggg acgttctctg gtgcgcttga tgtaagctgc tggtaaatag
     2461 aatcttgctc atatgccgga gcgttcgctg caagccaggc gttaatgacc tcgtccttgc
     2521 gatcaagaaa actctcaaat tcctgaagct gtgcatcact caacccttca gacagctggg
     2581 tgcctacgcg taattcaagt tgttccctaa agtgaccaag aaaaagcttc ttttggtctt
     2641 ctggaaggtc accaagacct acttcttgca gaaaattatc gtctaactgg aacatttgtc
     2701 attctccttc ttgccccaat tataccacct tacgcagtag ggttcgcccc accttgagca
     2761 gacgtatcag aaccagacgc ctgctcatca ccaagccgag catcgatctc ataaatgaga
     2821 tcgaggtatg catcaagcct accatcttca ggaagctgct cggcaatttc agaagcctgt
     2881 tgcatcactt cctttgaagt gtccccgaac gtataagccc cataagtact tctagacggc
     2941 ttacgggatc agtattgt
//
LOCUS       DATIIX010000119         7852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_388591, whole genome shotgun sequence.
ACCESSION   DATIIX010000119 DATIIX010000000
VERSION     DATIIX010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 7852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7852
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388591"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VLF64_03320"
     CDS             complement(<1..577)
                     /locus_tag="VLF64_03320"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HSW91003.1"
                     /translation="MPKKITKRRLRLQLTFVYGIMILAVVAIVTVLVLIIQGYRFNRY
                     DGRLEQGGLVQFDSQPSGATVMADSSELANKTASKIVLSAGNHTIAIGKDGYNTWKKD
                     VIVKPGTVLWLNYALLFPTNPKVSTAAKYPAVASALPSPNERIMAVLADATIPEITLT
                     TLDTDSPATTKLTVPASAYTQPVDGAVQSFSL"
     gene            715..1731
                     /locus_tag="VLF64_03325"
     CDS             715..1731
                     /locus_tag="VLF64_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW91004.1"
                     /translation="MKSTTVSINGTVYDSRTGMPLRKERSDADAVNHSAHNVHTQLQK
                     STTLNRRYVHHDTSPVAKPHVIYVPQRQRVAAPSANVMHRAPATQRQTPATRPQNQIT
                     HFAEHTPHPVAAPIADIGPTYHATVRRAKTRVEATRQVPERIVKPSQIIKQEAISRAM
                     AEATPKHEKAKQHHHHAKAHKKAHRSKIARFMSVASASVAVLLLGAYFTYLNMPALST
                     RVAAAQAGIGAGYPGYTPNGYSLNGPVAYQQGSVMMKFAANAGPQNYTLSQTHSAWDS
                     SALLEDYVAPQAGSNYITTTTGGLTIYSYNTTSVWVNNGILYTINGNSTLSDEQIQRI
                     AASL"
     gene            1774..3204
                     /gene="gltX"
                     /locus_tag="VLF64_03330"
     CDS             1774..3204
                     /gene="gltX"
                     /locus_tag="VLF64_03330"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HSW91005.1"
                     /translation="MTTRTRFAPSPTGFLHVGGIRTALFAYLVARQENGKFVLRFEDT
                     DKKREVAGSAEHLISSLRAIGIEYNEGPDIGGPFGPYVQSQRLERYKEWANKLIEQGR
                     AYADPYTPAEIQSFRDEAQTAKRAFRYRDHRPENPPIWDGSQPLRFKCDPKSFTYHDE
                     VMGDVTTSPEVVDDFILIKSDSYPTYNFAHIVDDADMEISHVIRGQEFISSMPNYLAL
                     YEALGLRHPIFAHMPHIMNEQGNKKLGKRDGAKDVLDYIRDGYLSETMMSFIATLGWN
                     DGTEQEVFTKDELIEKFSLARVQRSGARFDEKRLLWMNGHFIRELSLDELADRIEFFW
                     PESAKKASEAYKKRVLALAQDRIKTLRDLPGLTSYFFEEPTVNLALITENKQLKKLST
                     NETVNLLELVKNTFREVTDWTPEVLQDCLNQLLETTDQKPGTLFSLIRVVTTWAPFSP
                     QLNDTLALLGKNTTLARIDAATNHIQ"
     gene            complement(3185..4084)
                     /locus_tag="VLF64_03335"
     CDS             complement(3185..4084)
                     /locus_tag="VLF64_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HSW91006.1"
                     /translation="MPYQYRNPQYYRNAPEPVSRRRPLRKLTATFIVAAAVGAIIVSH
                     VEAQTAQTAVVEVSTKKQQVQMAVVRQAGTTIRNIIAADTKDTIGVAIENVDTGATTL
                     FGSPLQFEAASTEKVLSAITYYHLVEKGVLSLGAEVGIYPASYQLQQMINQSDNDSWS
                     AINAAVGGNDGLQAYANSIGLQYNIDGNLMSAKDMAVLLTKLYTGVLLNADHTKTLLS
                     YMQDTNDETLIPSVLPNALTIYHKYGELFDGTTNVLHDAAIVTDGSTTYAIVIYSDGK
                     TDEAARTETITRIAQAALTVCGW"
     gene            4184..5296
                     /locus_tag="VLF64_03340"
     CDS             4184..5296
                     /locus_tag="VLF64_03340"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HSW91007.1"
                     /translation="MIDVTPSKSTHSRWHKTRAWMHVHPQRTYAIVGFCIIVLAFIAA
                     ALVYAFAVPADKPIHIATVKKTPKKVASVVYYSPLTGIKVPDEATTKLPVTGIMIENS
                     PDARPQSGLKQAGIVYEAIAEGGITRFLALYQEAKPQLIGPVRSLRMYYLDWAAAYQA
                     SITHFGGSAASLAEVKNGQYRNLDLMAAGGQYYWRATDRYAPHNVYTTFAKLDALNAS
                     KNYTSSAFTPWPRQDGKPSAAPNATSVDIHISGPLYDSHYVYDATTNTYLRNVNGKPS
                     TDREDGQIAPTTLIAMKIDMSTVLQDGYREDIATTGSGQATIFQDGIATTATWQKKSR
                     TDPITFTDTAGKEIPLDRGQTWVAAVPNSSGSVSWQ"
     gene            5287..6843
                     /locus_tag="VLF64_03345"
     CDS             5287..6843
                     /locus_tag="VLF64_03345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSW91008.1"
                     /translation="MAVEQKQTGRREPQIRDYWRRYRRTARGIVILMEIVVTLIVGLA
                     LLFSDIDPTGTAFWATMTATLFATTALNFFILDFLLMPLRDLAMAVTNAAGEKPTDPL
                     ANPNTPRYSKNGFKPLLQFIYEHVHDGESAPNEKSENRENPQINHLETALRETNTGFI
                     VMDAHGKIQYANHAAPVKHDTNDTLQLELLFETNNTFVNWLANCRETAVRSEQSWMRV
                     PNRIVGDEERRIFNVTANYEKGSVAEVVIVAYDATNIFQPEDDELDFISFAAHELRGP
                     ITVIRGYLDVLEMELEDQLEPDQHELFKRLIVSANRLSGYINNILNTSKYDRRHLQVH
                     LSEENLGNIYDTIKDDMQLRAQSQRRLLSVDLPSSLPTIAADRSSISEVLSNLIDNAL
                     KYSNDGGSVSVGATIEGSFVKVSVTDNGIGIPSSVIGNLFHKFYRSHRSRETVAGTGI
                     GLYLCKAIVESHGGQIGVTSIEGQGSTFTFTVPVYASVKDKLQANDYTNAGLISSSEG
                     WIKNHSKYIS"
     gene            6864..7262
                     /locus_tag="VLF64_03350"
     CDS             6864..7262
                     /locus_tag="VLF64_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015642010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSW91009.1"
                     /translation="MAIKSILVIEDDRFIGEMYVRSLKHEGYDVDWVVDGHDGLVAVT
                     SKPYDLMMLDIMLPEMRGPEILKALRGGKVDKIPNTRVIVMTNFDQDDESRMAMQHDV
                     DAYLIKAEITPRKLIEVIKGFDTLTPVGAQ"
     gene            7423..7497
                     /locus_tag="VLF64_03355"
     tRNA            7423..7497
                     /locus_tag="VLF64_03355"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7456..7458,aa:Glu,seq:ctc)
BASE COUNT         2223 a         2140 c         1677 g         1812 t
ORIGIN      
        1 ccaagctaaa gctctgcact gcgccatcca caggttgcgt ataggctgat gctggcacgg
       61 taagcttcgt tgttgctggg ctatccgtat ctagcgtcgt caaagtgatt tctggtatag
      121 tagcgtcggc aagcacagcc attatgcgct catttggtga cggcaacgcg cttgcaactg
      181 ccggatattt cgccgcggta ctcaccttag gattagtagg gaataataag gcataattaa
      241 gccatagaac tgtgccaggc ttcacaatca catctttctt ccaagtgtta tagccatctt
      301 taccgatagc aatcgtgtgg ttacccgcag aaagcacaat cttgcttgcc gtcttattcg
      361 caagctcact actatcagcc ataactgtcg cacccgatgg ctgcgaatca aactgcacca
      421 aaccaccctg ttccaaccta ccgtcatagc ggttaaatcg atagccctga ataataagta
      481 caagcaccgt cacgatagca acaacggcta gtatcatgat tccatacaca aatgttagct
      541 gtaaacgcaa acgacgcttc gtaattttct ttggcatagt tattatatta taccacccac
      601 cgcaccactg tactaaccgg taaaataccg ggaagatcct cttgcataag cagttacgac
      661 aaagtatgat aaggtaagca cgagcagggt aggctcggag taactgggga atttatgaaa
      721 tcaaccaccg tatcaatcaa cggcactgtg tacgatagtc gcactggcat gccactacgc
      781 aaagagcgaa gcgatgcaga cgcggtcaat cacagtgcgc acaatgtcca tacgcaattg
      841 caaaaatcta cgacactcaa ccgtcggtat gttcatcacg atacgtctcc agtggcaaaa
      901 ccccatgtaa tttatgtgcc acagcgccag cgtgttgccg caccaagtgc aaatgtaatg
      961 caccgcgccc cagctactca acgtcaaact cctgctactc gtcctcaaaa ccagatcacc
     1021 cattttgcag agcatacccc acaccccgtt gccgcgccta tcgcagacat cggccccact
     1081 tatcacgcaa ccgtacggcg tgccaaaact cgcgtcgagg caacgaggca agtaccagaa
     1141 cgcatcgtaa agccttcgca gattattaag caagaagcaa tcagtcgcgc tatggccgag
     1201 gccacaccaa agcacgaaaa ggcaaaacag caccatcacc atgctaaagc tcataaaaaa
     1261 gcacatcgca gcaagattgc tcgctttatg agtgttgcta gtgcgtctgt tgccgtactg
     1321 ctgcttggtg catattttac atacctcaat atgccagcac tctcaactcg tgttgccgca
     1381 gcacaagcag gtatcggcgc cggctaccca ggctacacac caaacggcta cagtcttaat
     1441 ggcccagttg cttaccagca gggaagcgtt atgatgaaat ttgcggcgaa cgctggtcca
     1501 caaaactaca cgctgtcgca aacccactca gcttgggatt caagtgcgct tctcgaagat
     1561 tatgttgcac cgcaagccgg cagcaactac atcacaacaa ctaccggcgg gctgaccatt
     1621 tacagttaca acacaacatc agtttgggta aacaacggca tcctctacac aatcaacggc
     1681 aattcaacac tttccgacga gcagatccag cgcatcgcag caagtcttta gagcagtcgc
     1741 acattgcctt ataaataagg tataataaca gatatgacta cacgtactcg tttcgctcca
     1801 agcccaactg gctttttaca tgttggtggt attcgcaccg cactatttgc ttatctcgtt
     1861 gcccgccagg aaaacggtaa attcgtactg cgtttcgaag acaccgataa aaagcgtgaa
     1921 gtagcaggca gcgccgaaca ccttatttca agcctacggg caatcggcat tgagtacaac
     1981 gaaggcccag acatcggcgg cccatttggc ccatacgtcc aaagtcagcg tcttgaacgg
     2041 tataaagaat gggcgaacaa actaatcgag caaggccgtg cctatgccga cccttacacg
     2101 ccagcagaaa tccagagctt tcgcgatgag gcgcaaacgg caaaacgcgc ttttcgttac
     2161 cgtgaccacc gcccagaaaa tcctcccatc tgggatggct cacagccact tcgtttcaaa
     2221 tgcgacccaa aaagcttcac ttatcacgat gaagtaatgg gcgacgtcac gacaagccct
     2281 gaagtcgttg acgattttat tctcattaaa agcgatagct acccgaccta caatttcgcc
     2341 cacattgtcg acgatgcaga catggaaatc tcacatgtta tccgtggcca agagtttatt
     2401 agtagtatgc caaactacct tgctctatac gaagcactcg gtctgcggca cccaatcttc
     2461 gcacacatgc cacacatcat gaacgaacag gggaacaaaa aactcggtaa acgcgacggc
     2521 gccaaagatg tcctcgatta tattcgcgat ggctacctat cagaaacaat gatgagcttc
     2581 attgccacgc tcggttggaa cgacggcacc gaacaagagg tcttcacaaa agacgaactc
     2641 atcgagaagt tcagtcttgc cagggtacag cgcagcggcg ctcgcttcga cgaaaagcga
     2701 ctcctctgga tgaatggcca tttcattcgc gaacttagcc ttgacgagct cgctgatcgc
     2761 atcgaattct tttggcctga aagcgcaaaa aaggcgtccg aggcatacaa aaagcgagtt
     2821 cttgcacttg ctcaagatcg catcaagaca ctccgcgacc tcccaggact tacaagctat
     2881 ttcttcgagg aaccaacagt caatcttgct ctgattaccg aaaataaaca gctcaagaag
     2941 ctttccacta acgagaccgt caatctgtta gaattggtca aaaatacatt tagagaggtt
     3001 acagattgga cccccgaagt attacaagat tgcctcaacc aactccttga aactacagac
     3061 caaaaaccag gtacactctt cagccttatt agggtcgtca cgacctgggc gccctttagt
     3121 ccgcaactta acgacacgct tgctctactc ggcaaaaaca caacgcttgc gcgcatcgac
     3181 gccgctacca accacataca gtaagcgccg cttgagcaat ccgcgtaata gtttctgtac
     3241 gcgccgcctc atcagtctta ccatctgaat aaatcacaat tgcataggta gttgacccat
     3301 ctgttacgat tgccgcatca tgcagtacat ttgtggtccc atcaaaaagc tcaccgtatt
     3361 tatgatagat tgtcagcgca tttggcaaaa ctgatggaat caacgtctcg tcattcgtat
     3421 cttgcatata cgacaatagt gttttggtat gatcagcgtt aagcaatacg cccgtataca
     3481 gtttggtgag caatactgcc atgtcttttg cgctcattaa attaccgtcg atgttgtatt
     3541 gcagcccaat tgaattagca tacgcctgta gtccatcatt accaccaacc gcagcattga
     3601 tcgcactcca cgaatcatta tcgctctggt tgatcatttg ctgtaactga tacgatgcag
     3661 ggtaaatacc cacctctgca ccaagactca acacaccctt ttcaacaaga tgatagtagg
     3721 tgatagcgct caacactttc tcggtgcttg ccgcttcaaa ctgcaacggc gagccaaaca
     3781 aagttgtcgc gcctgtatcc acattctcaa tcgcgacgcc aatcgtatcc ttcgtatcgg
     3841 cggcaataat atttcgtatt gtcgtgcccg cttgccgtac aactgccatc tgcacctgct
     3901 gctttttcgt tgacacctca accaccgcag tctgtgctgt ttgcgcctca acatgactga
     3961 caataatggc cccaactgct gcagcaacaa taaaagtcgc tgttaattta cgtaagggac
     4021 gtctacgtga tacaggttct ggcgcgtttc gataatactg gggatttcga tattgataag
     4081 gcataaatct cttagtcacc ttagtccatg cacgcttaag acgccctgaa ggtatatccg
     4141 agcatcctat atagacctcc tctatgctat actaagcata agtatgatag acgtcactcc
     4201 gtccaaatca acccattcac ggtggcacaa aacccgtgca tggatgcatg tacatccaca
     4261 gcggacctat gcgattgtcg ggttttgtat aattgtccta gcgtttatcg cagcagctct
     4321 cgtttatgca tttgcggtac cagcagataa gccaatccat attgccacag tcaaaaagac
     4381 accaaaaaag gtggcgtcgg ttgtatatta ctcaccactc actggcataa aagtacccga
     4441 cgaggcaaca actaaattac cagtcacggg tattatgatt gaaaattcac ctgacgctcg
     4501 gccacaaagt ggactcaaac aagcggggat tgtgtacgaa gcaatcgccg agggtggcat
     4561 cacgcgcttc ctcgcactct accaagaggc aaaaccgcag ttaattggcc ctgtgcgcag
     4621 ccttcgtatg tattaccttg attgggcagc cgcctatcaa gcctcgatca cacactttgg
     4681 cgggagcgcc gcatcgcttg cagaagtaaa gaacggccaa taccgtaatc ttgatttgat
     4741 ggcagctggt ggccagtact actggcgtgc caccgaccgc tacgcgccgc acaacgtcta
     4801 cacaacattc gcaaaactcg atgccctgaa tgcatcaaaa aattatacct catcagcatt
     4861 tactccatgg ccacggcaag acggcaagcc aagtgcggcg cccaatgcaa cctcggtcga
     4921 tatccatata tctggcccgc tatacgattc acattatgta tacgacgcaa caaccaacac
     4981 gtacctgcgt aatgtaaacg gcaagccaag caccgatcgc gaagatggtc aaattgcgcc
     5041 aactactctc attgcaatga aaatagatat gtcaaccgtc ttgcaggatg gctatcgcga
     5101 agatattgcc accactggct ccggtcaagc aaccattttt caagacggca tcgctacaac
     5161 agctacttgg caaaaaaaga gtcgcactga cccaattaca ttcaccgaca ctgcaggcaa
     5221 agagattcca ctcgacagag gccagacctg ggttgcagcc gtgccgaatt ctagtgggag
     5281 cgtatcgtgg cagtagaaca aaagcaaaca ggtcgacgcg aaccacaaat ccgtgattat
     5341 tggcgccgct accgtcgcac tgcgcgcggc atcgtcattt tgatggaaat cgtcgtcaca
     5401 ttaattgttg ggctcgcact tctattcagt gacattgatc caacaggtac tgcgttctgg
     5461 gcgacaatga cagcaacact ctttgcgaca acagctctta acttcttcat tctggatttc
     5521 ctgcttatgc cactccgtga ccttgcaatg gccgtcacta atgccgcagg cgaaaaaccg
     5581 accgacccgc tcgcgaaccc taacacacca cgctatagta aaaacggttt taaaccacta
     5641 ctgcaattta tctacgaaca cgtacacgat ggagagtcag ctcctaacga aaagagcgaa
     5701 aaccgcgaaa acccacaaat taaccatctc gaaaccgcac tacgcgaaac aaacactggg
     5761 tttatcgtga tggacgcgca cggcaaaatt cagtatgcca accacgccgc cccagtcaag
     5821 catgatacca acgatacgct gcagctcgag cttctatttg agaccaacaa cacatttgta
     5881 aattggcttg ccaactgccg cgagacagca gtccgctccg agcaaagttg gatgcgagta
     5941 ccaaaccgca ttgtcggcga tgaagagcgt cgtattttca atgtaacggc aaactacgaa
     6001 aagggaagtg tcgccgaagt cgtaatcgtt gcgtatgatg ccactaatat cttccagccc
     6061 gaagacgacg aactcgattt catttcattc gcagcgcatg aactccgtgg tccgattaca
     6121 gtaatccgtg gctatctcga tgttctggaa atggaactcg aggatcagct cgagcccgat
     6181 caacacgagc tattcaaacg cctcatagtc agcgccaacc gcctctcggg ctacattaac
     6241 aacatcctca atactagcaa gtacgatcgc cgtcacttac aggtacatct ttcggaggaa
     6301 aacctcggca atatttacga cactatcaaa gacgacatgc agctccgcgc acagtcacaa
     6361 cgtcgtttgc tttctgttga tctcccgtca agcttgccaa caattgccgc cgaccgctca
     6421 agcattagcg aagtgctttc aaaccttatt gataacgcac tcaagtactc taacgacggt
     6481 ggctcagtat cagtcggcgc aacaatcgaa ggcagtttcg tcaaggtctc cgttacagac
     6541 aacggcatcg gtattccatc aagtgttatt ggcaacttat ttcacaaatt ctaccgcagc
     6601 caccgctcac gcgaaaccgt tgcaggcacc ggtatcggtc tttacctctg taaagcaatt
     6661 gtcgaaagtc atggtggcca aatcggggtc acaagtatcg aaggccaagg gtcaaccttc
     6721 acgtttaccg tgccagtcta cgcatcggtc aaagataagc tccaagcaaa cgattacact
     6781 aacgctggcc ttatttcatc aagcgaaggc tggattaaga atcactcaaa atacataagc
     6841 taaagtgtta taatagaggc actatggcaa ttaagagcat cctggttatc gaagatgatc
     6901 gattcatcgg cgaaatgtac gtccgcagcc tcaagcatga gggatacgac gttgactggg
     6961 ttgtcgacgg acacgacggg cttgtggcgg taaccagcaa accctacgat cttatgatgc
     7021 tcgacattat gctccctgaa atgcgtggcc cagagattct caaggcactt cgcggcggta
     7081 aggtggacaa aatcccgaac acccgcgtta tcgttatgac taacttcgac caagacgatg
     7141 aaagccgcat ggctatgcaa catgatgtcg acgcatacct cattaaagcc gaaattaccc
     7201 cacgcaaatt gatcgaggta atcaaaggct ttgacacact cacaccagtg ggtgcgcagt
     7261 agacaaaagc ggttacatct gttaatatga tctgagtcaa tcctttacgg gttgataaaa
     7321 actcaggaaa tacgttatat ggctccatca tataagacgc aaatgcctcg gaatagcaca
     7381 aaaatagtaa acacattttt tgccgctctt cactcagaat ttggccttat cgtctaacgg
     7441 ttaggacaca aggttctcat ccttgcaatc cgggttcgat tcccggtaag gtcaccaaag
     7501 aaagaacgtc accatttggt ggcgttcttt ctttgggcga atttatccgc cgaatcgaac
     7561 ccgttgcggt tcgctattat ctggccgttt actgcaaaag gcgtaatata ccttagtatt
     7621 cccggtgagg tcaccaagtt tctatcaaat cctttttgtt ctgaatgcat gcacgctcac
     7681 tcaaagggcc ttacgattga cattacagtt acctagtgtt ataataatat aaccatcagg
     7741 gagagtctct ggcggttgca cacagaaggg tcaataccat gaccaccaat cagatcaaga
     7801 ctcagaccac caccggctgc accacggaga atccgttctc gcctcagcgg ct
//
LOCUS       DATIIX010000120         5741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Saccharimonadales bacterium isolate
            SMAG_U15676 k141_54890, whole genome shotgun sequence.
ACCESSION   DATIIX010000120 DATIIX010000000
VERSION     DATIIX010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936390
            Sequence Read Archive: SRR9179561
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Saccharimonadales bacterium (soil metagenome)
  ORGANISM  Candidatus Saccharimonadales bacterium
            Bacteria; Candidatus Saccharimonadota; Candidatus Saccharimonadia;
            Candidatus Saccharimonadales.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 06:25:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 679
            CDSs (total)                      :: 654
            Genes (coding)                    :: 652
            CDSs (with protein)               :: 652
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Candidatus Saccharimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54890"
                     /isolate="SMAG_U15676"
                     /isolation_source="soil"
                     /db_xref="taxon:2973664"
                     /environmental_sample
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.426944 N 8.518333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..306)
                     /locus_tag="VLF64_03360"
     tRNA            complement(230..306)
                     /locus_tag="VLF64_03360"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(270..272),aa:Arg,seq:tct)
     gene            complement(383..457)
                     /locus_tag="VLF64_03365"
     tRNA            complement(383..457)
                     /locus_tag="VLF64_03365"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(422..424),aa:Gly,seq:tcc)
     gene            579..1040
                     /locus_tag="VLF64_03370"
     CDS             579..1040
                     /locus_tag="VLF64_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW91010.1"
                     /translation="MGFFRSRFNNNSARQLFHSSGVVSDADGEKIGSINSESFEQRQK
                     VEGNRRLVGGYRNAGIVHTYRQEAHDALFSQQGNSDDKDKKYHRTSMVTDRPTSYIQN
                     PSSSRIDIIKPSSRIARDTGITKPGIGPTKPTTGVKRTFMEPVTRKYNPYQ"
     gene            complement(1088..1164)
                     /locus_tag="VLF64_03375"
     tRNA            complement(1088..1164)
                     /locus_tag="VLF64_03375"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1128..1130),aa:His,seq:gtg)
     gene            complement(1252..2781)
                     /locus_tag="VLF64_03380"
     CDS             complement(1252..2781)
                     /locus_tag="VLF64_03380"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HSW91011.1"
                     /translation="MSTAKYLRNIGTLVQETRQARGMTQAELATALGTSQSAINRIEK
                     GGQNISLEMIARIGEVLSSEIVRLNKSGKTNFVISGGNELHGEIEVKTSKNAAVGLLC
                     AALLNKGKTTFHRVARIEEVNRIIEVLESIGVRCRWLDGNDLEITPPKVLKLDDIDNA
                     AAKRTRSIIMFLGPLLHQYKDFKLPFAGGCNLGTRTVEPHMVGLAPFGLSVEARAGTD
                     YYHATVEARSPERAIVLTERGDTVTENIIMAAALYDGEVVIRNASPNYMVQDVCFFLQ
                     KLGVKIEGIGTTNLKIHGVKSINKTVDYYPSEDPIEAMSFVAAGVVTNSEITVKRVPI
                     EFMELELATLGGMGLEYTQTDEYPARNGQTRLVDISLKKSELHSPKDKLHAMPFPGIN
                     MDNLPFLGLCATVATGRTLLHDWSYENRAIYFTELSKLNASIEMVDPHRVYITGPTKW
                     KPADITAPAALRPSVVILLAMLAAPGKSILRDVYSINRGYEDIANRLNTLGAEIETTW
                     E"
     gene            2906..4189
                     /gene="glyA"
                     /locus_tag="VLF64_03385"
     CDS             2906..4189
                     /gene="glyA"
                     /locus_tag="VLF64_03385"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HSW91012.1"
                     /translation="MNDDIVAQLIAGEANRQREGLELIPSENYVSSDVLAALGSVFTN
                     KYSEGYPGKRYYGGQEFTDQVEQLAIDRAKQLFGADHANVQPHSGAPANEAVYSAWCE
                     PGDTILAMDLSHGGHLTHGSPVTRSAHLYNFVRYKMKDPATGAIDYDELRKVALEHRP
                     RIILAGFSAYPRELDYQKFADIAHEVGEDCLLMADMAHIAGLIAGGAAKNPLDYGFHV
                     ITTTTHKTLRGPRGGMILSMGKVGNPLKAPEKKLENIPTLIDRAVFPGMQGGPHMHTI
                     AAKAVAFGEALKPEFREYAAQIVKNAAKLAEELEKRDFQLVTGGTSNHLILADVNKSF
                     GIDGKVAEVALDKIGLTLNANSVADDPLPMYRPSGIRLGTPAATTRGLKEEHMMQIAE
                     WMKQAIDNRKDEAKLAGLRAEVHEFMGAFPLPTDK"
     gene            complement(4294..4761)
                     /locus_tag="VLF64_03390"
     CDS             complement(4294..4761)
                     /locus_tag="VLF64_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSW91013.1"
                     /translation="MTKQIKTSGFTIVELLIVIVIIAILAAITIVAYNGITQRANTSS
                     AKSAAENAIKKIEAYNADSSVQTYPKTFAALTGAASTASYQLTGVTFTTTRATGSTAP
                     STPSTLNFFICTTGSAVEYFDYTAKAWTAVFTGGATGIGGTAGTTADCAYQAS"
     gene            complement(4851..>5741)
                     /locus_tag="VLF64_03395"
     CDS             complement(4851..>5741)
                     /locus_tag="VLF64_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSW91014.1"
                     /translation="GVITTLPAGFRPNKQIIFTTISSGSNAIARVDVFPDGTVMWQGG
                     GDNSWVSLDGINFMSSDFDASTNWVNPTLLCPSGCTTPWTDFSGGSTSYGRLQIGKDS
                     IGRAQLYGILSAPSPYPPANNSNMATLASAYSVSTNDIYPDFASSNVYAAYGIISNAL
                     QFRTNLGNNWKTFAAMYYPSGSSATWTSIPLSSSWVPYNTAGSGYSQPQFTESTDHIV
                     TLRGLIRSGTASAGTTVGTLPAGAHPATNLIFIVSGYNGTAEIPVRVDVLATGQIVIR
                     TTIANNYLSFANISFYQDGN"
BASE COUNT         1542 a         1298 c         1419 g         1482 t
ORIGIN      
        1 gaggaatata cagttgcaaa tttaggtagg tgcacagaat attcctctgc ggcagcttca
       61 ccttgcgcgg tgagtgtgtc atcttttcca tcccagttgg cgtgccttag cacgtatact
      121 tctttcatct tcctttaatg ataacagatg gatgtcaagt ttctaactca aaaatggtgt
      181 gtgagatgtc aaaactaaat ttaaaggttg aaataatgaa ttctaaagtt ggtaccccga
      241 gctgggttcg aaccagcgac cttaggctta gaagtcctct gctctatcct gctgagctat
      301 cggggcattg tcataattat agcaaggaca tccgaataaa tgaaaaatag acgctagtaa
      361 tgcgcctaca ataaatatct attggtgcgg gtggggggaa tcgaaccccc gtcccaagtt
      421 tggaaaactt gtatactaac cgttgtacta cacccgcaaa gtctaacgta ttgtagcatt
      481 tttattgcgc ataggaaagc aggtggaaat gataagcaaa agagccttta acgtttatta
      541 tacatttttg ctagtctgca ctagggtata ataaaagaat gggtttcttc cgttcacgat
      601 ttaataataa ttcagcgcgt cagctattcc attctagtgg tgtagtcagt gatgccgatg
      661 gcgagaagat tggcagtata aacagcgaat cttttgagca gcgtcaaaag gttgagggta
      721 atcgacgtct tgttggtggc taccgcaacg cagggatagt acacacctat cggcaagaag
      781 cgcacgatgc acttttcagc cagcagggga atagtgacga caaagacaaa aaataccatc
      841 ggacctcgat ggtcactgat cgtccgacct catatatcca aaatccctca tcgagccgaa
      901 tcgatattat aaagccatca tcacggattg cgcgcgatac tggtatcaca aaaccaggga
      961 tagggccaac aaaaccgacc acaggcgtaa agcgcacatt catggagcca gtaacacgaa
     1021 aatataatcc gtatcaataa aatgcgccca tagaaaaaga cttatcgcag tgataagtct
     1081 ttttctatgg ggcgaataat ggggttcgaa cccacggcct ccggagccac aatccagcgc
     1141 tctaaccagc tgagctatat ccgccataaa aatctagttt tcactaaact cctctggatt
     1201 ataccagata tagcgcgata tgtcatctgt tatttaggaa ttgtgggaat cttattccca
     1261 ggtagtttca atctcggcgc caagtgtatt gagacgattg gcgatgtctt cgtagccacg
     1321 gttgatgctg tagacatcac gcaggattga tttaccaggt gccgcaagca ttgcaagcaa
     1381 aattacgaca cttgggcgga gtgctgctgg tgccgtaata tccgctggct tccactttgt
     1441 tgggccagtg atatacacgc ggtgtgggtc aaccatttca atactagcat tcagtttaga
     1501 aagctcggta aagtaaatag cgcggttctc gtaactccaa tcgtgaagta gtgtccgtcc
     1561 ggtcgctacg gttgcgcaca gtccaaggaa tgggaggtta tccatattaa taccggggaa
     1621 tggcatggcg tgaagcttgt cttttggcga atggagctcg gatttcttga gtgaaatgtc
     1681 aacgagacgg gtctgaccgt tgcgggcagg gtactcgtcg gtctgcgtat actcaagtcc
     1741 catgccgcca agtgttgcca gctcgagctc cataaactca atcggtacgc gctttacagt
     1801 gatttcagaa ttagtcacaa cgccagccgc cacaaaactc atagcttcga tcgggtcttc
     1861 gctaggatag tagtcaacag ttttattaat tgatttcaca ccgtggatct tgagattggt
     1921 cgtaccaata ccttcaatct ttactccgag cttttgcagg aaaaagcaaa catcctgcac
     1981 catgtagtta ggacttgcgt tacggatgac tacttcaccg tcgtaaagcg cagctgccat
     2041 aataatgttc tcggtgaccg tatcaccgcg ctcggtaagt acaatagcac gctcaggact
     2101 gcgagcctcc actgttgcat ggtagtagtc ggtacccgcg cgggcttcaa cgcttagacc
     2161 gaacggcgca aggccgacca tgtgtggctc aactgtacgc gtaccgaggt tgcagccacc
     2221 agcaaatggg agtttaaaat ctttgtattg atggagcagc ggaccaagga acatgatgat
     2281 tgagcgggtt cgcttagcgg cggcattgtc aatatcatca agtttgagca cctttggcgg
     2341 agtaatctcg aggtcgttgc catcaagcca acggcatctt acgccgatac tctcgagtac
     2401 ctcgataata cgattcactt cctcaatgcg tgcaactcgg tgaaaggtag ttttcccctt
     2461 attgagaagt gcggcacaga ggagcccaac agcagcattt ttactagttt ttacctcgat
     2521 ttccccgtgg agctcgttgc caccactaat aacaaagttc gttttaccgg acttattaag
     2581 ccgaacgatt tcgcttgaaa gcacctcacc gattcgagct atcatctcaa ggctgatatt
     2641 ttgtccgccc ttctcaatgc gattaatcgc cgattggctg gtgccaagag cggttgctag
     2701 ttctgcctgg gtcatcccac gggcttggcg cgtttcctgc acgagtgtac cgatattacg
     2761 taaatacttt gctgtactca tacatctata atatatctga tgtgatatat ttgcaataca
     2821 ctacctgtgg tatactaaag gtatgattta acactacgac gtatcaccac agcaacaaat
     2881 agaatccaaa agaggggaat tttatatgaa tgatgatata gtcgcacaac ttattgcagg
     2941 tgaagcaaac cgccagcgcg aaggtcttga gctaatacca agtgaaaatt acgtgagcag
     3001 cgacgttcta gcagcgcttg gtagtgtgtt tactaataaa tatagcgaag gctacccggg
     3061 caagcgctac tatggtggcc aagagtttac agaccaggta gaacaactag ctattgatcg
     3121 tgcaaagcag ctatttggcg cagaccatgc gaatgtgcag ccacacagcg gcgcacccgc
     3181 aaacgaagcg gtatatagcg catggtgcga gccaggggat acaatccttg cgatggattt
     3241 gagccatggt ggtcacttaa cacacggctc acctgtcact cgtagcgcac acttatataa
     3301 tttcgttcgt tacaagatga aagatcccgc aacaggtgca atagactatg atgaattgcg
     3361 taaagttgcg ctcgagcacc gcccccggat tattttggca ggatttagcg cgtacccacg
     3421 cgagctggac tatcagaagt ttgcagatat cgcccacgaa gtaggcgagg attgtttgct
     3481 aatggcggac atggcacata ttgccggcct gattgctggt ggagcagcta aaaacccact
     3541 cgattatggc ttccatgtta tcaccacgac aactcataag acacttcgtg gcccacgtgg
     3601 gggcatgatt ctttctatgg ggaaggtagg aaatccgcta aaggcacccg agaagaagct
     3661 cgaaaacatc ccgacactta ttgaccgcgc ggtcttccct ggtatgcagg gtggcccgca
     3721 catgcatacg atcgctgcta aagcagtggc atttggcgag gcattaaaac cagagtttcg
     3781 tgaatacgct gcacaaatag taaagaatgc cgcaaagctg gccgaagagc tcgaaaagcg
     3841 cgacttccaa ctggtaacgg gtggaaccag caaccatttg attctagcag atgtaaacaa
     3901 gagcttcggt atcgatggaa aggttgccga ggttgctctt gataagatcg gtctaacgct
     3961 taatgcaaac tcagtggcag acgacccgtt gccaatgtac cgtccaagcg ggattcgcct
     4021 cggcacacca gcggcgacga ctcgcggcct caaagaagag catatgatgc agattgctga
     4081 atggatgaaa caagcgattg ataatcgtaa ggatgaagcg aagcttgctg gcttgcgtgc
     4141 tgaagtccat gaatttatgg gcgcatttcc attgccgaca gataagtaac tttcgcgtgt
     4201 ggttataaac tcgtcacaga taaaagtggt gagtttttct gtataagaaa tgagcgccat
     4261 atggcgctca tttcttatgt ggaggcaaaa tccttatgat gcctgatacg cgcagtcggc
     4321 tgttgtacca gcagtaccgc cgataccagt cgcaccacca gtaaagacgg ctgtccatgc
     4381 cttagctgtg tagtcaaagt attcaactgc tgaacctgtc gtacagataa agaagttaag
     4441 agtacttggt gtacttggcg cggtgctacc tgtagccctt gtagtggtga aagtcacacc
     4501 agtaagttgg taacttgcgg tagatgccgc acccgttagc gctgcaaatg tcttaggata
     4561 tgtctgaaca ctggagtctg cattatatgc ttcaattttc ttgatcgcgt tctcggccgc
     4621 agacttcgca ctcgaagtgt ttgctcgctg cgtaataccg ttgtatgcaa cgatcgtgat
     4681 cgcagcaaga atagcgatga ttacaataac gatgagaagc tcaacgattg tgaatccgct
     4741 tgttttaatc tgtttggtca tttaagacgc cccctaatta atatcttata tacgtatatg
     4801 gtatcaaagt gcttatgctc gcgcaatagg taaatacagt tgactacgaa ttagttacca
     4861 tcttgataga agctgatatt ggcaaacgaa agataattat ttgctatggt agtcctaatt
     4921 actatctgtc ccgttgctag gacatcgacg cgcactggta tttctgcggt accattatat
     4981 ccagatacaa tgaagataag attagtagcg gggtgcgctc cagcagggag cgtgccaacc
     5041 gttgtaccgg ctgaggcagt tccgctccta ataaggccgc gtagagtgac aatatgatcc
     5101 gttgattcag tgaattgcgg ttgcgaatag ccgctgccag cagtattgta gggtacccag
     5161 ctacttgata gcggtataga tgtccatgtt gctgacgatc cgcttgggta atacatggca
     5221 gcaaatgttt tccagttatt gccaagatta gtgcgaaact gcagggcgtt actaatgatg
     5281 ccgtatgccg cataaacatt agatgaggca aaatctggat agatgtcgtt ggttgagaca
     5341 gaatacgctg aagcaagcgt ggccatattt gagttgtttg ctgggggata tggggatggt
     5401 gcggagagga tcccataaag ttgtgcccgg cctatgctat ctttaccaat ctgtaatctt
     5461 ccgtaacttg ttgagcctcc actaaagtct gtccaggggg tagtacatcc gctcgggcag
     5521 aggagcgtcg ggttaaccca gtttgtcgat gcgtcaaagt ctgatgacat aaaattgata
     5581 ccatcaaggc ttacccagct gttgtcgcca ccgccctgcc acatgactgt gccatcagga
     5641 aatacgtcga cacgtgctat tgcgtttgag ccgcttgata tggtggtaaa gatgatttgc
     5701 ttgtttggtc ggaaccctgc cggcaatgta gtgatgactc c
//
