LOCUS       DATIOW010000001         3697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1524067, whole genome shotgun sequence.
ACCESSION   DATIOW010000001 DATIOW010000000
VERSION     DATIOW010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524067"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..829
                     /locus_tag="VLL03_00005"
     CDS             116..829
                     /locus_tag="VLL03_00005"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSS45790.1"
                     /translation="MPRCVEPEWLDELPADDPRAIRSRRDLQRVNVWMLQVGIMERAL
                     AKHCAYDKPHVLLELGAGDGSFMLRLAQRLAPRWKNVKVILLDRQNIVSRETREGFST
                     LQWKIETVTADVFDFLAQAGHQSVDVITANLFLHHFPNQKLIQLLAQAAPITGLFVAC
                     EPRRTMPALLGSRLLWAIGCNEVSRHDAVVSVRAGFSGQELSALWPKNGEWRTHEHAA
                     RLFTHCFVARRCIVQGTTT"
     gene            826..2037
                     /locus_tag="VLL03_00010"
     CDS             826..2037
                     /locus_tag="VLL03_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HSS45791.1"
                     /translation="MKHDALIIGAGPAGGAAALMLAQAGWSVAVVEKSGFPRRKVCGE
                     FISATSLPLLHELGVAGSFLEIAGPEVRRVGLFAGETIVTSAMPQPRGPLERWGRALG
                     REHLDMFLLTAASQAGARVWQPWTATELQRAKNGYICKIAAKGTSKELHAPIVIAAHG
                     SWERGTLSAQSINRAHKPSDLLAFKAHFRECDVPSDLMPLLIFPGGYGGMVHSDGGRA
                     SLSCCIRRDELRRCRHRQKQHAAEAVLQHIQASCLGVRQALRRASLDGGWLSAGPIRP
                     GIRKRYLNGIFLVGNAAGEAHPLVAEGINMAMQSAWLLCQRLIASQDDIAAERAVAEV
                     GKDYAAEWSTGFAARIRAAAVFAHLAMRPNAAVLLLPVLKRFPGVLTFGAQLSGKARP
                     VVSAPYPRTAL"
     gene            2095..2847
                     /locus_tag="VLL03_00015"
     CDS             2095..2847
                     /locus_tag="VLL03_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HSS45792.1"
                     /translation="MSKTLHVATYNIHKGFSHFNRRMMVHELRERLHAMRADVVFLQE
                     VQGIHDHHAVRYANWPCTPQYEFLADSVWSNFSYGRNAIYDAGHHGNAILSRFPIVRW
                     DNQDISAHPLEHRGLLHCELKIAGWNEYLHCINVHLGLFARWRKKQLLALGGRIEELV
                     PPHAPLIIAGDFNDWRGQAGRVFAEALELHEVFEQIRGRPARSFPSVIPLFRLDRIYV
                     RGFCVERAHVHHGRPWSKISDHAALSATMRRV"
     gene            2895..>3697
                     /locus_tag="VLL03_00020"
     CDS             2895..>3697
                     /locus_tag="VLL03_00020"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HSS45793.1"
                     /translation="MHFVAGNKLTLLHNGVKYFPELEAAIDGAASEIFLETYIFENDV
                     AGKRIAAALERAAKRGVIVHLVVDGFGSRTLPQEMIDAMSEAGVQVLVFRPEVMSFRF
                     RRNRLRRMHRKIVVIDSEIAFVGGINIIDDMHTPKQVPPRFDFAVKIEGPLLRAIHTA
                     AKDLWAGAARTHFKRAWRNTLGRPAVIRPRGEQRAALVTRDNVRHRRDIEQAYLKAIN
                     SAKQEILIASAYFLPGRNLRRALVSAAQQGVRVILLLQARVEYVLLHYAS"
BASE COUNT          713 a         1030 c         1174 g          780 t
ORIGIN      
        1 ggcggcgctc atggaaaacg cgccgggcgg gtggtggtgg ttgtcctctt ttggcgccgg
       61 cttcagttgc catggcgcct tgctggaagt ggcttagaca gaagatttca tgagcatgcc
      121 gcgttgcgta gagcccgagt ggctggatga attgccggcg gatgatccac gcgccatccg
      181 ctcgcggcgt gacctgcagc gggtcaacgt ctggatgctg caggtgggca tcatggagcg
      241 cgcgctggcc aagcactgtg cgtacgataa gccgcacgtg ctgctcgagc tcggcgccgg
      301 cgacggcagc ttcatgttgc gactcgcgca gcggctcgcg ccgcggtgga aaaacgtcaa
      361 agtaattttg ctcgatcggc agaacatcgt cagccgcgaa acccgggaag gtttcagcac
      421 actgcaatgg aaaatcgaaa cggttacagc ggacgtgttt gattttctgg cgcaggcagg
      481 gcatcagagc gtggatgtca ttacggccaa tctgtttctg catcactttc cgaatcaaaa
      541 attgattcaa ttgctggctc aagccgcgcc aatcacgggt ttgtttgtgg cttgtgagcc
      601 gcggcgcacc atgccggcgc tccttggcag ccgattgttg tgggcgatcg gttgcaatga
      661 ggtaagccgc catgacgcgg tggtgagcgt gcgtgccggt tttagcggtc aggaactctc
      721 cgcattgtgg ccgaagaatg gcgaatggcg gacgcacgag cacgcggcaa ggcttttcac
      781 gcattgtttt gtggcgcggc gttgcatcgt acaaggaaca acaacgtgaa acatgatgcg
      841 ctcatcattg gcgcaggccc tgccggaggg gccgcggcgc tgatgcttgc ccaagccggc
      901 tggtcggtgg ctgtggtgga aaaatccggt tttcccaggc gcaaagtctg cggcgaattc
      961 atttcggcta ccagcctgcc gttgcttcac gagctcggcg ttgccggttc ttttctagaa
     1021 attgccgggc cggaagtgcg gcgggtcggt ttgttcgccg gagaaacgat tgttacttcg
     1081 gccatgccgc aaccgcgggg ccccctcgaa agatggggca gagccctggg gcgggagcat
     1141 ctcgatatgt ttttactcac cgctgcgtcg caagccggag cgcgagtgtg gcaaccatgg
     1201 acagccacgg aattgcagcg tgccaagaac gggtacatct gcaaaatagc ggcaaaagga
     1261 accagtaaag aacttcatgc gccaattgta attgccgcac atggttcgtg ggagcgcggc
     1321 acattgtctg cgcaaagcat taaccgggca cacaagccct ccgacttgct cgccttcaag
     1381 gcgcacttta gggaatgtga tgtgccctct gatctgatgc cgctgctgat ttttcccggt
     1441 ggctatggcg ggatggtgca tagcgacggc ggacgcgcaa gcctctcctg ctgcatccgc
     1501 cgcgatgagt taagacgatg ccgtcaccgg cagaaacagc atgcggcaga agcggttttg
     1561 cagcatattc aagcttcctg cctgggcgtg cgccaggccc tgcgccgcgc cagtctggat
     1621 ggtgggtggt tgtctgccgg acccatccgg ccgggaattc gcaagcgtta cttgaatggc
     1681 atttttctgg ttgggaatgc tgccggagaa gcgcatcccc tcgttgccga gggaatcaac
     1741 atggcgatgc aatcggcgtg gctgttgtgc caacgcctta ttgcgagcca agatgatatt
     1801 gcggcagagc gggctgtcgc tgaagtcgga aaagactatg cggcagagtg gagcacaggc
     1861 tttgccgcgc gcatccgcgc cgccgcagtg tttgcccatc tcgcgatgcg gccaaatgca
     1921 gcagtcctgc tgttgccggt actgaagcgg tttcctggag ttttaacttt tggggcacaa
     1981 ttaagcggca aggccaggcc ggttgtttct gcgccgtacc cacgaacggc tttataatgt
     2041 tcaaatcacg acctctttac ttcatctgac cgggaaagcg cataaaaacg cgttatgagc
     2101 aagacgctgc acgttgcgac ctacaatatc cacaagggct tttcgcactt caaccggcgc
     2161 atgatggtac atgagctgcg cgagcgcctg cacgcgatgc gcgccgacgt ggtgttcctg
     2221 caggaagtgc agggcatcca cgaccatcat gccgtgcgct atgcaaactg gccatgcacg
     2281 ccgcaatacg aatttctcgc cgattcggta tggagcaatt tcagctacgg ccggaatgcg
     2341 atttacgacg cggggcacca cggcaacgca atcttaagcc gctttcccat tgtgcgctgg
     2401 gacaaccagg acatttccgc gcatccgctc gagcaccgcg gactcctgca ctgcgaattg
     2461 aaaatcgccg gctggaatga atatctgcac tgcatcaacg tgcatctggg cttgtttgcg
     2521 cgctggcgca aaaaacagtt gctggcgttg ggtggacgaa ttgaagagct ggtgccgccg
     2581 catgcgccgc tgattatcgc cggcgatttc aacgactggc gcggccaggc cggacgggtt
     2641 ttcgccgagg cgctggaact gcatgaggtg ttcgagcaga tccggggccg gccggcgcgg
     2701 agtttcccct cggtgattcc cttgttccgg ctggatcgca tttacgtgcg gggtttttgt
     2761 gtcgagcgcg cgcacgtgca tcacggccgg ccgtggtcga aaatttccga ccacgccgct
     2821 ttgtcggcga ccatgaggcg cgtgtgacgc tttcggcggt tgagcgccaa gccgaatcca
     2881 tttcctgatt tcacatgcat ttcgtcgcgg gcaacaagct gacgctgctt cacaacggtg
     2941 tcaaatattt tcccgagctg gaagcggcga ttgacggcgc ggcttcggaa atctttctcg
     3001 aaacctatat tttcgagaat gatgtggccg gcaaacgcat cgcggcggct ctcgaacgcg
     3061 ccgcaaagcg cggcgtcatc gtgcatctgg tggttgacgg cttcggctcc cgcacgctgc
     3121 cgcaggagat gatcgacgcc atgagcgaag cgggagtgca ggtgctggtg ttccgcccgg
     3181 aagtgatgag tttcaggttc aggcgcaacc ggctgcggcg catgcaccgg aagattgtgg
     3241 tgattgacag cgaaatcgct ttcgtcggcg gcatcaacat cattgacgac atgcatacgc
     3301 cgaagcaggt gccgccgcgc ttcgatttcg cagtgaagat tgaagggccg ctgttacggg
     3361 ctattcacac cgcggcgaag gatttgtggg cgggcgcagc gcgcacgcat tttaagcgcg
     3421 cctggcgcaa caccttgggg cggccggcgg tgattcggcc ccgcggtgaa cagcgcgcgg
     3481 cgctggttac gcgcgacaac gtgcgccacc ggcgcgacat cgagcaggct tatctcaagg
     3541 ccatcaactc cgcgaaacag gaaatcctca tcgccagcgc ctatttcctg cccggccgca
     3601 atttgcgccg cgcgctggtg agcgcggcgc aacaaggcgt gcgcgtgatt ttgctgttgc
     3661 aggcgcgggt cgaatatgta ttgctgcatt acgcttc
//
LOCUS       DATIOW010000002         6580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1829744, whole genome shotgun sequence.
ACCESSION   DATIOW010000002 DATIOW010000000
VERSION     DATIOW010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6580
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829744"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1245
                     /locus_tag="VLL03_00025"
     CDS             160..1245
                     /locus_tag="VLL03_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSS45794.1"
                     /translation="MALLEIRNVTRRFGELTAVNNVSINIEAGEFFTLLGPSGCGKTT
                     LLRMIAGFDLPDGGQILLDGKDLAGTPPEKRPIHTVFQSYALFPHMTVTGNVVFPLKM
                     AGKPQEEIQAKVKEVLDLVHLSDKGESFPHELSGGQKQRVALARGLVNRPRLLMLDEP
                     LGALDAKLREEMQIELISLQRDIGVTFVFVTHSQAEALALSHRIAVMNAGVVEQIDEP
                     SKIYGFPVNRFVADFIGNINMLDAEVVETASEHLRLHIPGMGEVRAPAKTGAAPGQKG
                     VLALRPEQVRIAAKIEDAANDNHFPGKVRDFLYIGDITTYIVDLNNGARVEALLPNSA
                     PGRAKFFEIGDTVEVAWRRDAGIFLNA"
     gene            1238..2113
                     /locus_tag="VLL03_00030"
     CDS             1238..2113
                     /locus_tag="VLL03_00030"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSS45795.1"
                     /translation="MPNLLNPARVTKWLISGPPFLYLFLFFLLPSLIMVVASFRYPGE
                     FGGLAKGTELTLENYQFFFSDFIYTQIFIKSFGVALATTLICLLMAYPLALLIAHSPK
                     RHRDFMVLLVILPFASNFLIRVYAWMIILGPQSAFSLVINHFLGLFGIGPVPLLFSSF
                     AVLVGMVYVHLPFMVQPLYTNLEKHDPVLLDAAQDLGANAWDRFWKVTWPLSLPGVYS
                     GAALVFIPVLGMFAIPDILGVTGDILIGNLIKDQFLGTRDWPFGSTLSIMLTIAVLLL
                     AGITAWISRRGVRNA"
     gene            2106..2885
                     /locus_tag="VLL03_00035"
     CDS             2106..2885
                     /locus_tag="VLL03_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSS45796.1"
                     /translation="MRRSAMLWIIAALVYAFLYVPLVIVVVYSFNDSRLNAEWVGFTL
                     NWYSVLFNDEQMIGAAINSLTIAVVSSLVATALGTMAGIAIHRYKLKFLPFMVITPVA
                     MPEILLGVSLLLFFIQVLNLTLGMLSIIIAHITFCIGFVAIVVRARLAGMDESIFEAA
                     RDLGATPWQSFRLVTFPLIMPGVIAGVLMSFTLSIDDFVITFFTVGVGVTTLPLQIYS
                     MIKIAVTPEVNAVSTLLMLLTLTTIIIASKFAPDALRGGAK"
     gene            2882..3940
                     /locus_tag="VLL03_00040"
     CDS             2882..3940
                     /locus_tag="VLL03_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HSS45797.1"
                     /translation="MKRGLFLVFLLFSGGAPAEDVLHLYNWNNYISEGTVGRFEKFCN
                     CRLVQDYYGDNEEMLAKLAAGATGYDLIVPTQNAVDSLIKQDALRPLDKSLLPNLKNL
                     KPEFLNRDFDPGNKYSVPYAYSTTVVGYNEQKMQELGLPTDTWAVIFEPKYLQKVKGR
                     VTVLDSQRELMAAAMKYLGYSINDTDEEKWKRARDLIIRAKPYWAAFNAQSYIKELTV
                     GNIWLAHGYSNDFFQAALDAKKAARKFSIGWRIPKEGAVIGLDSMVLHKFGPRPDLAH
                     KFINFMLEGENSAELTNLIGAGNPNAAALPYIKPEIAQNPAIFPDPETTKHLEFMKDF
                     DRKQRRVMSRMWTEIKLK"
     gene            complement(3942..4634)
                     /locus_tag="VLL03_00045"
     CDS             complement(3942..4634)
                     /locus_tag="VLL03_00045"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HSS45798.1"
                     /translation="MKKANRILPGLILACLLVAENAVRAEEPPRYNEVDLQAEAQREI
                     QNDVMAVTLYAEQNGENPAQVANAVNKIVNSALRTAAEVKGVKAVTGGYQTYAIYGKN
                     NRLESWRVRSEIRLESKDFGAASNLIGKLQSSMQLSYLGFTVSPEARRQAENEMIAEA
                     IAKFRSRADIVKQSINGRSYKIRRISVNTSGFHPRPVLARALAAQEVAAPNVEGGVSQ
                     VTVNVNGMIEVE"
     gene            complement(4651..5091)
                     /gene="mscL"
                     /locus_tag="VLL03_00050"
     CDS             complement(4651..5091)
                     /gene="mscL"
                     /locus_tag="VLL03_00050"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HSS45799.1"
                     /translation="MLKEFKEFAMRGNVVDMAVGIIIGAAFGAIVQSLVADVIMPPIG
                     LILGNVDFSNLFVVLKEGAAAAAPYTSLAEAQKAGAVTVNYGKFLNSVVSFIIVAFAV
                     FMLIRALNRLKKQEEAKPVTRECPQCFSVISIKAKRCPHCTSQL"
     gene            complement(5542..>6580)
                     /locus_tag="VLL03_00055"
     CDS             complement(5542..>6580)
                     /locus_tag="VLL03_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HSS45800.1"
                     /translation="KNIKTEYEKVRQQHKDKKGQPLLRLAEARSNRFKIDWGSCVLPK
                     PRFTGRKEFRGYPLKEIVPCIDWTPFFQTWELAGRYPAILQDAVVGEAARNLFQDAQA
                     MLKLIIEEKWLTANAAIGLFPANSAGDDVEIYTDESRTIAAMTFHNLRQQNVKPKGNP
                     NQCLADFIAPKETGIKDYIGAFAVTTGIGIDARVKAFEEKHDDYNAIMLKALADRLAE
                     AFTEHLHLRVRREFWGYAPDENLDSEALISEKYRGIRPAPGYPACPDHTEKPALFELL
                     KAQEVGITLTESFAMLPAAAVSGFYFSHPESGYFAVGKIGKDQAEDYARRKEMSLAEA
                     EKWLAPVLAYD"
BASE COUNT         1314 a         1964 c         1787 g         1515 t
ORIGIN      
        1 ggcgtgagct acagctacag tttttaacct ggtgcagccc gcgagaataa atcaaagatg
       61 ccaatacaat tcgcaacaaa cattttaggt gtggcgcaat tcagttccta tattttcaac
      121 atttgctaga ataagcgaca ttcatcggcc ggattttaca tggcgcttct ggaaatccgc
      181 aatgtgacgc ggcgcttcgg cgaattgacc gcagtcaaca acgtcagcat caatatcgag
      241 gcaggagaat tctttaccct gctcggccct tcgggttgcg gcaagaccac actgctcaga
      301 atgatcgccg gattcgattt gcccgatggc ggccagattc tgctcgacgg caaggacctc
      361 gccggcacgc cgccggagaa gcgtccgatc cataccgtgt tccagagtta cgcgctgttt
      421 ccgcacatga cggtaacggg taatgtcgtg tttccgctta agatggcggg caagcctcag
      481 gaggaaattc aagccaaagt caaagaggtg ctggatttgg tgcatctctc ggacaaaggc
      541 gaaagtttcc cgcatgaact ttccggaggc cagaagcagc gcgttgcgct cgcccgcggc
      601 ctggtgaacc gcccgcgcct attgatgctc gacgaaccgc tgggcgcgct cgatgcgaaa
      661 ctacgcgagg aaatgcaaat cgagctcatc agtctgcagc gcgacatcgg cgtcaccttc
      721 gtgttcgtca cgcactcgca ggccgaggcg ctggcgctct cgcaccgcat cgcggtgatg
      781 aatgcgggcg tagtcgagca gatagacgag ccctcgaaaa tctacggctt tccggtcaac
      841 cgcttcgtcg ccgactttat cggcaacatc aacatgctcg atgccgaagt ggtggagacg
      901 gcctccgagc atttgcgcct gcacattccc ggcatgggcg aagtgcgggc gccggccaaa
      961 accggcgccg cgcccggcca gaaaggggtg ctggcgctgc gcccggaaca ggtacgcatc
     1021 gccgccaaaa tcgaggatgc ggctaatgac aaccactttc ccggcaaggt gcgcgacttt
     1081 ctttacatcg gcgacatcac cacctacatc gttgacctca acaatggcgc ccgcgtcgaa
     1141 gcgctgctgc ccaactcggc gccgggacgt gctaaattct tcgaaatcgg cgacaccgtc
     1201 gaagtggcgt ggcgacgcga cgcgggaatc tttctgaatg cctaatttgt tgaaccccgc
     1261 ccgcgtcacc aagtggctca tcagcgggcc gccgtttctt tatctttttc ttttcttcct
     1321 gctgcccagc ctcatcatgg tggtggcttc tttccgttat cccggagaat ttggcgggct
     1381 cgccaaggga accgagctca cgctggagaa ttatcagttt ttcttcagcg actttatcta
     1441 cacccagatt ttcatcaagt ccttcggcgt ggccctcgcc accacgctga tatgcctgtt
     1501 gatggcatac ccgctggcgt tgttgattgc acacagcccg aagcggcacc gcgacttcat
     1561 ggtgctgctg gtgattctgc ccttcgccag caattttctg attcgggtct acgcctggat
     1621 gattatcctc gggccgcaat cggcgttcag cctagtgatt aatcatttcc tggggctttt
     1681 cggcatcggg ccggtgcctt tgcttttctc ttcgttcgcg gtgctggtcg gcatggtcta
     1741 tgtgcacctg ccgttcatgg tgcagccgct ttacaccaat ctggaaaagc atgacccggt
     1801 attgctggat gccgcgcaag atctgggcgc caacgcctgg gaccgtttct ggaaagtcac
     1861 ctggccgctg tctttgcccg gcgtgtattc cggcgcggcg ctggtgttta tccccgtgct
     1921 cggcatgttc gccattcccg acatccttgg cgtcaccggc gatatcctca tcggcaacct
     1981 gattaaagac cagttcctcg gcacgcgcga ctggcctttc ggcagcaccc tttccatcat
     2041 gcttaccatc gccgtgcttt tgcttgctgg catcaccgca tggatcagcc gcaggggtgt
     2101 aagaaatgcg tagaagcgcc atgctctgga tcatcgcggc gctggtttac gccttcctct
     2161 acgttccgct cgtcatcgtc gtggtgtatt cgttcaacga ctcaaggctc aacgccgaat
     2221 gggtgggctt cacgcttaat tggtacagcg ttttgttcaa cgacgagcag atgataggcg
     2281 cagcaatcaa ttcgctcacc atcgccgtcg tttccagcct cgtggccacc gcgcttggca
     2341 ccatggcggg catcgccatt caccgctaca agctgaagtt tctgccgttc atggtcatta
     2401 cgccggtcgc catgccggaa atcctgctcg gcgtttcgct gctgctgttt ttcatccagg
     2461 tgctgaacct cacgctgggc atgctttcca tcatcattgc gcacattacg ttctgcatcg
     2521 gctttgtcgc catcgtggtg cgggcgcggc tcgccggcat ggacgagagc attttcgagg
     2581 cggcgcgcga tctcggcgca acaccgtggc agagcttcag gctggtcacc ttcccgctca
     2641 tcatgcccgg cgtgattgct ggtgtgttga tgtcgtttac cctctcgatt gacgattttg
     2701 ttattacctt cttcaccgtg ggcgttggcg tgaccacgct gccgctgcag atttattcga
     2761 tgatcaagat cgcggtcacg ccggaggtga acgccgtctc cacgttgctc atgttactca
     2821 cgctcaccac catcatcatc gcctcgaaat tcgcgcccga tgcgctgcgc ggcggcgcaa
     2881 aatgaaacgc gggttgttcc tcgtttttct tctgttttcc ggcggcgccc cggcggaaga
     2941 cgtattgcac ctctataact ggaacaatta catctccgaa ggcacggtcg ggcgtttcga
     3001 gaaattctgc aattgcaggc tggtgcagga ctattacggc gacaacgagg aaatgctggc
     3061 gaagctcgcc gccggcgcga ccggctacga tctcatcgtg cctacccaaa acgcggtgga
     3121 ttcgctcatc aagcaggacg cgttgcgtcc cctcgacaag tcgcttttac ccaatctaaa
     3181 aaatctcaag cctgaattcc tgaatcgtga tttcgatccg ggcaacaaat actcggtgcc
     3241 ttacgcctat tccaccacgg tcgtcggcta caacgagcaa aagatgcaag agctggggct
     3301 gcccaccgac acctgggcgg tgattttcga gccgaagtat ctgcagaaag taaaaggccg
     3361 cgtcaccgtc ctcgattcgc agcgcgagct gatggcggcg gcgatgaaat atctgggcta
     3421 ttcaatcaac gacacagacg aggaaaaatg gaagcgggcg cgcgacctca taatccgcgc
     3481 caagccgtac tgggcggcgt tcaacgcgca gagctacatc aaggaactga cggtgggcaa
     3541 catctggctc gcgcacggct attccaacga ttttttccag gcggcgctag atgcgaagaa
     3601 agctgcgcgg aagttcagta tcggttggcg gattcccaag gaaggcgcag taattggcct
     3661 ggacagcatg gttctgcaca aattcgggcc gcggcctgat ctcgcgcaca agttcatcaa
     3721 ttttatgctc gaaggcgaaa attccgccga gctcaccaat ctgatcggcg cgggcaaccc
     3781 caacgccgcg gcgctcccct acatcaagcc ggaaatcgcg cagaatcccg ccattttccc
     3841 cgatccggaa accaccaaac atctggaatt catgaaagac ttcgaccgta agcagcgccg
     3901 ggtcatgtcg cgcatgtgga ccgagatcaa attgaagtga cctattcgac ttcaatcatg
     3961 ccgttcacat tgactgtaac ctggctcacg ccgccttcca cattgggcgc cgccacttcc
     4021 tgtgctgcca gcgcccgcgc cagtaccggg cgcggatgaa aaccgctggt gttgacgctt
     4081 atcctgcgta ttttgtaact gcgcccgttg atcgattgtt tgacgatatc ggcgcggctg
     4141 cggaatttgg cgattgcttc ggcaatcatt tcattttccg cctgcctgcg cgcttcgggt
     4201 gaaactgtaa aaccgagata agaaagctgc atgctggatt gcagtttgcc tattagattg
     4261 gaagcggcgc cgaaatcctt gctctccagc cggatttccg agcgcacgcg ccagctttcg
     4321 agccgattgt tcttgccgta aatcgcataa gtctgataac caccggtcac ggcttttaca
     4381 cctttaacct ccgccgccgt gcgcaaagcg ctgttgacga tcttgttgac ggcattggcg
     4441 acctgcgcgg gattctcgcc gttctgctcc gcatacagcg tgaccgccat cacgtcgttc
     4501 tggatttcgc gctgagcttc cgcctgtaaa tctacctcgt tgtaacgcgg cggctcttcc
     4561 gcccgaactg cattttctgc caccagcagg caagcgagta ttaaccctgg caaaatacga
     4621 ttagcttttt tcatctggcc tccgagccga tcagagctgt gaagtacaat gcgggcaacg
     4681 cttggctttg atggaaatga cggaaaagca ttgcgggcat tccctggtaa ccggcttggc
     4741 ttcttcctgt tttttaagcc ggttgagcgc gcgaatcagc atgaacaccg caaacgccac
     4801 gatgatgaaa ctcaccaccg agttgagaaa tttcccgtaa ttgacggtca ccgccccggc
     4861 tttttgcgct tcggcgagcg aagtatacgg cgccgcggcc gccgcgccct ctttcagcac
     4921 cacaaacaag ttggagaaat cgacattgcc gagaatgagg ccgatcggcg gcataatgac
     4981 atcggccacc agcgactgca cgatggcgcc gaacgccgcg ccgatgatga tgccgaccgc
     5041 catgtccacc acattgccgc gcatggcgaa ttccttaaac tccttaagca tggcttccct
     5101 cccgtggaat taaccggccc tcatcatacc ttaagacagc ggagtatatg gggcgcggcc
     5161 ggacggcatc acttctccga ccataatctc atctctgcct attttttgcc ccttctgagc
     5221 cgccgagtac cgcaggcgag ccgggaagtg gtcggcgagc gctgtttgag cgcacagcgc
     5281 gagttgcgca gccgccccgg cttgtcgagg aacataggga accgcttagc ggcggcgaag
     5341 cagggtggcc ttttctttgg tgactttctt ttgggcaagc aaaagaaagt aaccagacgc
     5401 cggggcgcct cggatcacgg tgagcgcagc tttgtgcgga gacgaggcgc agcggctgca
     5461 tgaaatatat ttctccgagg cttttgcggc cgctcccgca ctcgggcttc gcctaccgtg
     5521 tcgcggccgc cccccggcaa cttaatcata agccaacacc ggcgcaagcc acttttccgc
     5581 ttcagccaaa ctcatttctt tgcggcgcgc atagtcctcg gcctggtcct taccgatttt
     5641 gccgacggcg aaatagcctg attcgggatg cgagaaataa aacccgctta ctgccgcggc
     5701 gggcagcatg gcgaaacttt cggtaagcgt gatgccgacc tcctgcgctt tcaataattc
     5761 aaacaacgcc ggcttttcag tgtgatccgg gcaagccggg tagccgggcg cggggcggat
     5821 gccgcggtat ttctcggaaa tcagcgcttc gctgtcgaga ttctcatccg gcgcataacc
     5881 ccagaattcg cgccgcaccc gcagatgcaa gtgctcagta aacgcttcgg ccaggcggtc
     5941 ggcgagcgct ttcagcatga tggcgttgta gtcatcgtgc ttttcctcaa acgccttgac
     6001 ccgcgcgtca atgccgatgc ctgtggtcac ggcgaacgcg ccgatatagt ccttgatgcc
     6061 tgtctctttc ggcgcgatga aatcggcgag gcattggttg ggattgcctt tcggcttcac
     6121 gttctgctgg cgcaggttgt gaaacgtcat cgccgctatg gtgcgcgatt catcggtgta
     6181 aatctcgacg tcatcacccg ccgagtttgc cggaaacaat ccgattgccg cgttcgcggt
     6241 cagccatttt tcctcgatga taagcttcaa catcgcctgt gcatcctgga acagattgcg
     6301 cgcggcttcg cccaccactg catcctgcaa aatcgccgga taacggcccg cgagctccca
     6361 ggtctggaaa aacggcgtcc aatctatgca gggaacgatt tccttaagcg gatacccacg
     6421 gaactctttt cggccggtga agcgcggttt aggcaacacg cagcttcccc agtcgatttt
     6481 gaagcggttg cttctcgctt cggccagcct caaaagcggc tgtcctttct tgtccttatg
     6541 ttgctgccgt actttttcat attcggtctt gatatttttc
//
LOCUS       DATIOW010000003         1681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1119341, whole genome shotgun sequence.
ACCESSION   DATIOW010000003 DATIOW010000000
VERSION     DATIOW010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119341"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VLL03_00060"
     CDS             complement(<1..693)
                     /locus_tag="VLL03_00060"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010114575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HSS45801.1"
                     /translation="MKERLIIFDTTLRDGEQSPGASMTREEKVRIARQLERMRVDVIE
                     AGFPAASNGDFESVQAVAKTIKDSTVCALARAIEADVERAGTALKGAAAARVHTFIAT
                     SPIHMQKKLRMSPDQVLEQAVKAVQWARKYTDDVEFSPEDAGRSEVDFLCRVLEAVIK
                     AGASTLNIPDTVGYNVPDQFGKLIRQLRERIPNSDKAVFSVHCHNDLGLAVANSLSAV
                     LQGARQVECTING"
     gene            complement(921..1679)
                     /gene="pssA"
                     /locus_tag="VLL03_00065"
     CDS             complement(921..1679)
                     /gene="pssA"
                     /locus_tag="VLL03_00065"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HSS45802.1"
                     /translation="MSGKLPKRGIYLLPNLFTTAALFAGFYAIVQAVNGLFEHSAIGI
                     LVAMVLDGLDGRVARLTHTQSAFGAEYDSLSDMVSFGAAPALVAYVWALQTMGRLGWI
                     AAFVYCAGAALRLARFNTTTEVEDKRFFQGLPSPAAAALIAGLVWVMNDYQISGGDVR
                     WLAWGVTVFAGLSMVNNLRFYSFKDINLRKSVPFVFMLLLVLSFVLISVDPPTVLFSG
                     FVAYALSGYVMWLWELRKNKPVAGASSGDKSLPD"
BASE COUNT          381 a          511 c          476 g          313 t
ORIGIN      
        1 gccgttgatg gtgcattcca cctggcgcgc tccctgcaat accgcgctca gcgaattcgc
       61 caccgccagg cccaggtcgt tatggcaatg caccgaaaac accgccttgt ccgagttggg
      121 aatgcgctcg cgcaactggc gaatcagttt gccgaactgg tcgggaacgt tgtagcccac
      181 ggtatcggga atattgagcg tgctcgcccc ggccttgatc accgcttcca gcacgcggca
      241 caggaaatcc acttccgagc gcccggcgtc ttcaggtgaa aactccacat cgtcggtata
      301 tttgcgcgcc cactgcaccg cctttaccgc ctgctcgagc acttgatccg gtgacatgcg
      361 cagctttttt tgcatgtgga tgggagaggt ggcgatgaag gtatgcacgc gcgcggcggc
      421 tgcgcccttg agcgctgtgc ctgcacgctc gacgtccgcc tcgatggcgc gcgccagtgc
      481 gcacacggtg ctgtccttga tggtcttggc gaccgcctgc accgactcga agtcgccgtt
      541 gctcgccgcc ggaaaaccgg cttcgatgac atccacgcgc atgcgctcga gctgcctggc
      601 gatgcgcacc ttctcctcgc gcgtcatgga agcgccgggg ctctgctcgc cgtcgcgcaa
      661 ggtagtgtcg aatataatca aacgttcttt catgtctttc tccgctagaa aatgctggaa
      721 attataatgc agaaaccggc tggcggtttc tgaagctcaa agcaaaactg aatggaagag
      781 tagaagtatt tagcgcgcaa ggcgcagcag cagacctgcc agcagaacgc cggcaaggcg
      841 ggaaactaag cttatgctgc aaatggaact catggtccag gaaatataat gcctttttgg
      901 cgaatatgca agaagcgggc ttaatcgggc agtgatttat ccccggagga cgcgccggca
      961 acaggtttgt tctttctcaa ttcccacaac cacatgacat aaccggagag cgcgtaggcg
     1021 acgaagccgc tgaacaagac agtcggcgga tcgactgaaa tcaagacgaa tgacagcacc
     1081 agcagcagca tgaatacgaa aggcacgctc ttgcggagat tgatgtcctt gaagctgtaa
     1141 aaacgtaagt tgttcaccat ggagagtccg gcaaacaccg tcacccccca ggcgagccag
     1201 cgcacatccc cgccgcttat ttgatagtcg ttcatcaccc acaccagtcc cgcgatcagc
     1261 gccgcagcgg cggggctcgg caatccctgg aagaaacgct tgtcctccac ctcggtggtg
     1321 gtattgaagc gcgcgagcct gagcgccgct cccgcgcagt aaacgaaggc ggcaatccat
     1381 cctagccggc ccatggtctg cagcgcccaa acgtaagcta ccagcgccgg ggccgcgccg
     1441 aaggatacca tgtcggaaag gctgtcatac tcggcgccaa aagcgctctg ggtatgggta
     1501 agccgcgcaa ccctgccatc cagcccgtcg agcaccatcg ccaccaagat gccgatggcg
     1561 gaatgctcga ataggccgtt cactgcctgg acaatcgcat agaatccggc aaacaacgcc
     1621 gccgtggtaa aaagattagg cagcaaataa atcccgcgct tggggagctt gcccgacagc
     1681 a
//
LOCUS       DATIOW010000004         2404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_815163, whole genome shotgun sequence.
ACCESSION   DATIOW010000004 DATIOW010000000
VERSION     DATIOW010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815163"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VLL03_00070"
     CDS             complement(<1..219)
                     /locus_tag="VLL03_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HSS45803.1"
                     /translation="MSSPQLVLASASIYRRELLERLQLPFSAAAPNVDESPLPGEQPE
                     QTAARLAQAKALAVKNLYPQALIIGCDQV"
     gene            275..781
                     /locus_tag="VLL03_00075"
     CDS             275..781
                     /locus_tag="VLL03_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HSS45804.1"
                     /translation="MPEQVAVDSLAFARGAKALSGKIPVADLLRLRDCLYSTAGVIEY
                     ELRGTVNSRGKPQLHLVIQGRICLRCQRCLGELAQDVERVCDLLLIENQNDFPEVTEE
                     DETVDCIPAETELDVLALLEEEIILGLPLSPRHDTAACRISEEYDGSRAGRTAFAPLA
                     ALKNRNTE"
     gene            791..970
                     /gene="rpmF"
                     /locus_tag="VLL03_00080"
     CDS             791..970
                     /gene="rpmF"
                     /locus_tag="VLL03_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HSS45805.1"
                     /translation="MAVQQNKKSPSKRGMHRAHDFLTNPPLAVEPTTGEVHLRHHISP
                     NGYYRGKKVTRGKGE"
     gene            1004..1996
                     /gene="plsX"
                     /locus_tag="VLL03_00085"
                     /pseudo
     CDS             1004..1996
                     /gene="plsX"
                     /locus_tag="VLL03_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764798.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
     gene            2020..>2404
                     /locus_tag="VLL03_00090"
     CDS             2020..>2404
                     /locus_tag="VLL03_00090"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191963.1"
                     /note="FabH; beta-ketoacyl-acyl carrier protein synthase
                     III; catalyzes the condensation of acetyl-CoA with
                     malonyl-ACP to initiate cycles of fatty acid elongation;
                     differs from 3-oxoacyl-(acyl carrier protein) synthase I
                     and II in that it utilizes CoA thioesters as primers
                     rather than acyl-ACPs; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HSS45806.1"
                     /translation="MYSRIVGCGSYLPKKILTNRDLETMVDTSDEWIFTRTGIRQRHI
                     AADNENVSDLALNASKIAMQTSAVKAGDIDLIIVATTTPDMIFPSTACILQSKLGIKN
                     CPAFDVQAVCSGFVYALATADLFIRS"
BASE COUNT          522 a          657 c          729 g          496 t
ORIGIN      
        1 gacttggtcg cagccaatga tgagcgcttg cgggtatagg tttttcaccg ccagggcttt
       61 agcttgggcc agacgagcgg cggtttgttc gggctgctcg cccggcaagg gcgattcatc
      121 aacgttcgga gcggcggcgg agaaggggag ttgcaggcgc tctaacagct cgcggcggta
      181 aatcgaagcc gaggccaaaa ccagctgtgg ggagctcaaa tttttctcgt aagtttttga
      241 caccaaactg caaaaataat atcatgcgcg ccttatgcct gaacaggtcg ctgtcgacag
      301 cctggcattt gcccgcggcg cgaaagctct aagtggtaaa atacccgtcg ccgacttgct
      361 gcgtttgcgc gattgtttgt attccacggc aggtgtcatc gaatacgagt tgcgcggcac
      421 ggtaaattcc cgaggcaagc cgcaattgca tctggtgatt caaggcagga tttgcctcag
      481 atgccaacgc tgcctgggcg aactggcgca ggatgtggaa cgggtttgcg atctgctgct
      541 gatcgaaaac caaaatgatt ttcccgaagt tacggaagaa gatgaaaccg tagactgcat
      601 cccggcggaa acggagctgg atgtgctggc cttgctcgag gaagaaatca tattggggct
      661 gccgctttct ccgaggcatg acacggcggc atgtcgcatc agtgaagaat atgatggcag
      721 ccgtgctggc cgcacggctt ttgcgccgct cgcggcactg aaaaatcgga acactgaata
      781 ggagtttgtc atggcagtcc agcaaaacaa gaaatccccg tcgaagcgcg gcatgcaccg
      841 tgcgcatgat tttctgacca acccgccgct ggcggtcgag cctaccaccg gtgaagtcca
      901 tttgcgccac cacatcagcc ctaacggcta ttaccgtggc aagaaagtga ctcgcggcaa
      961 gggcgaatag tcgccaaacc gggcgacccc tgcctgcacg ggaatggata tcacggtagc
     1021 cattgattgc atgggcggtg accacgggcc gcaggtcacg gtgccggcag cggtaaaatt
     1081 ccagcggcgc aatcccgata cgcaaatcgt cttggtcggc ctgcgcgacg tgatcgaacc
     1141 ggagttgcgc gccatgcgcg ccgaaaccgg cccgcggttg cgcgtgcatc acaccagcga
     1201 aatagtgacc atggatgaac agccggtcct cgccctgcgt ggcaagaaag attcctcgat
     1261 gcgcgtggcg atcaatctgg tgaaaaacgg cgaagcccag gccggcgtca gcgccggcaa
     1321 cactggcgcg ctcatggcca tttcccgttt tgtgctgaaa accctgccgg gaatcgaacg
     1381 ccctgcgatc gccaccgcgc tgcccagctt gcgcggtcaa acctatgtcc tagatctggg
     1441 cgccaacgcc gattgcaccc cggaacactt gctgcagttc ggcatcatgg gcgctaccct
     1501 ggtttccgcg gtggagcaca acgaacgtcc gagtgtcggc ttgctcaaca tcggcgaaga
     1561 agaaatcaag ggcaacgaag tggagaaacg ggcggcggaa ctgttgcgtg cgagcgggct
     1621 caatttctac ggcaatgtcg agggcgacga tatttataaa ggcactactg acgtcgtggt
     1681 gtgcgacggt tttgtcggta atgtggcgtt gaagacttcc gaaggcatgg cgcagatgct
     1741 ggagaccttc ctgcgcgagg aattcgagag cagttttttg actaggataa gcgccttttt
     1801 cgccatgtcg gcgcttaaag cttacaaacg ccgggtggat taccgccgtt acaatggcgc
     1861 gagtctcctt ggtttgcgcg gcgtggtggt aaaaagccat gggtcggcgg acagctttgc
     1921 ttttggtttt gccatccggc gcgcggtgga agaagtgcaa aacggcgtcc tgcggcgcat
     1981 cagcgagcgc atggcgctga ttcacccgga gccggtttga tgtactcgcg cattgtcggt
     2041 tgcggcagtt acctgccgaa gaaaatcctc accaaccgcg atcttgaaac catggtggac
     2101 acctcagacg agtggatttt cacccgtact ggtatccgcc agcgccatat cgcggcagac
     2161 aacgaaaacg tgagtgatct ggcgcttaac gccagcaaga tagcgatgca aacgtcggcg
     2221 gtgaaggcgg gcgatatcga cttgattatc gttgccacca ccacgccgga catgattttc
     2281 cccagcaccg cctgcatctt gcagtccaag ctcggtatca agaactgccc ggctttcgat
     2341 gtgcaagcgg tgtgcagcgg ttttgtttat gccttggcca ccgccgacct gtttattcgt
     2401 tcgg
//
LOCUS       DATIOW010000005         4133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_612172, whole genome shotgun sequence.
ACCESSION   DATIOW010000005 DATIOW010000000
VERSION     DATIOW010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612172"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="VLL03_00095"
     CDS             <1..867
                     /locus_tag="VLL03_00095"
                     /inference="COORDINATES: protein motif:HMM:NF008935.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HSS45807.1"
                     /translation="ITRLCYCGSVAHTLPAASTRTREPLQIGAELYGHAGLESDSEIQ
                     HLTLQALKLAGIAEVHLDVGHVAVFRSIAKRGEIPTELEAELFGVLQSKDMPALRELT
                     RSLEQRVSQALLLLPELYGGDEVLQRAAKHLPDYPEISAALADLRTLAKELRDKVASL
                     CFDLAELRGYRYHSGVVFGAYAKASANAIALGGRYDEVGKAFGRARPATGFSMDLREV
                     SALLPGTPLPAGILAPYVQDAALQKKIESLREQGEIVIVDLPGHENSRRELNCDRTLV
                     SRKGVWEVSSLN"
     gene            879..2171
                     /locus_tag="VLL03_00100"
     CDS             879..2171
                     /locus_tag="VLL03_00100"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HSS45808.1"
                     /translation="MSKNVVVVGTQWGDEGKGKVVDWLTDHAQGVVRFQGGHNAGHTL
                     VIGGKKTVLHLIPSGILRKGVNCYIGNGVVVSPQALLDEMAMLKKAGVEVIGRLKISE
                     ACPLILPYHAALDQAREAAKGARKIGTTGLGIGPAYEDKAARRAIRLQDLFHRERFTA
                     KLREVLDYHNFVLKNYFHAKPVDFQRTLEDTLALAGQIKPLVADVPRLLYQASREGKN
                     LLFEGAQGTLLDIDHGTYPYVTSSNCVAGAAAAGSGIGPQMLHYVLGITKAYTTRVGS
                     GPFPTELSDEVGKKLASRGQEFGATTGRPRRCGWFDAAALKRSIQINGVSGLGVTKLD
                     VLDGMRSLCLGVGYKIEGVDSDILPVGAELLAESEPVYEEIPGWLESTVGVKRFEDLP
                     RPAQNYLKRIEEICDVPIDMISTGAEREETIVLRHPFA"
     gene            complement(2272..2358)
                     /locus_tag="VLL03_00105"
     tRNA            complement(2272..2358)
                     /locus_tag="VLL03_00105"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2322..2324),aa:Leu,seq:cag)
     gene            2429..>4133
                     /gene="rnr"
                     /locus_tag="VLL03_00110"
     CDS             2429..>4133
                     /gene="rnr"
                     /locus_tag="VLL03_00110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HSS45809.1"
                     /translation="MTKAERSRSSPAKPKLRELDPHFKRERSKYREALPSREFILQIL
                     AEQGVPLSVEKLMQSLEVADHEVALFQRRLRAMERDGQIMRNRKGAICVVDKLDLVKG
                     TVQGHPDGFGFLIPDEGGPDLFLGPKEMQKVLHGDRVMAREIGTDRRGRPEASIVEVL
                     TRANQRLVGRLHVEHGIQFVVAENKRISQDILVPQGESHAAKPGQVVMAEIIEQPSKH
                     EQPVGRVVEVLGNYADPGMEIEIALRKHDLPHVFPPDAEISAKHLPSEVLEKEYAGRQ
                     DLRGLPLVTIDGENAKDFDDAVYCEAQGKGFKLFVAIADVSHYVKPGDALDREALQRG
                     NSVYFPRRVIPMLPEALSNDLCSLNPRVARLTMVCEMQIAADGEIGEYRFYPAVMLSQ
                     ARLTYTAVAEMLEHPEGDTARRFGRLLPHIKILYALYKVLAKAREKRGAIDFETIETQ
                     MIFNEHGKIERIVPVKRNDAHRLIEECMLAANVCASDFLKSRGQRTLYRIHEGPTPEK
                     LDTLHGFLREFALHLGGGDNPHAKDYARLLSTDRDRPDAQLLQTVMLRSLQQAMYSPD
                     NV"
BASE COUNT          910 a         1125 c         1274 g          824 t
ORIGIN      
        1 atcacccgtt tgtgctattg cggcagcgtg gcgcacactc tgcctgcggc atccacgcgc
       61 acccgcgagc cgctgcagat cggcgcggag ctttacggcc acgccggatt ggagagcgac
      121 agcgagattc agcacctgac gctgcaggcg ctcaagctag cgggcattgc ggaggtgcac
      181 ctcgatgtgg gccatgttgc ggtatttcgc agtatcgcca agcgagggga gattccgacc
      241 gagcttgaag cggagctttt cggggtgctg caatccaaag atatgcccgc tttgcgcgag
      301 ctgacgcgca gcctcgagca gcgcgtcagc caagcgctgc tgttgctgcc cgaactttat
      361 ggcggcgatg aagtgttgca gcgagcagcc aaacacctgc ccgattatcc ggaaatttct
      421 gcggcgctcg cggatttgcg cacgctggcg aaagagctgc gggataaggt agccagcctg
      481 tgctttgatc tggccgagct gcgcggttat cgctaccaca gcggcgtggt gttcggcgca
      541 tacgccaaag cctcggctaa cgccattgcg ctgggcgggc gttatgacga agtgggcaaa
      601 gctttcgggc gcgcgcggcc cgcgaccgga ttcagcatgg atttgcgcga agtttcggcg
      661 ctgctgccgg gcacgccttt gccggcggga atacttgcgc cttatgtgca ggatgcggcc
      721 ctgcaaaaaa agatcgagag cttgcgcgaa cagggcgaga tcgtgatcgt ggatttgcct
      781 ggacatgaaa actcgcgccg cgaactcaat tgcgaccgca ctttggtatc gcgcaaagga
      841 gtttgggaag taagcagcct gaactaatcg aatcgggtat gtcaaaaaac gtcgtcgtcg
      901 tcggcacgca atggggtgat gaaggtaagg gcaaggtcgt ggactggctc accgaccatg
      961 cccagggcgt ggtgcgcttc cagggcggac ataatgccgg ccatacgctg gtgatcggcg
     1021 gcaagaaaac cgttttgcac ctgattccct cgggcatcct gcgtaagggt gtcaactgct
     1081 atattggcaa tggcgtggtg gtttcgccgc aagccctgtt ggatgaaatg gccatgctaa
     1141 aaaaagccgg agtggaagtc attgggcggt taaaaatcag cgaagcctgt ccgctgattc
     1201 tgccttacca tgcggcgctc gatcaggcgc gcgaagcggc aaaaggcgca cgcaaaatcg
     1261 gcactaccgg actcggcata ggccccgctt acgaagacaa ggcggcgcgg cgcgccatcc
     1321 gtttgcagga tttgtttcac cgcgagcgct ttactgccaa gcttcgcgaa gtgctggact
     1381 accacaattt cgtgctgaag aattatttcc acgcaaagcc agtggatttc cagcgcactc
     1441 ttgaagacac gctcgcgctt gccgggcaaa tcaagccgct ggttgcggat gtgccgcgcc
     1501 tgttatacca ggcgagccgc gaggggaaaa atctcttgtt tgaaggcgcg caaggcacgt
     1561 tactcgacat cgaccacggc acttaccctt acgtcacttc cagcaattgc gtggcaggcg
     1621 ccgccgctgc gggaagcggc attggaccgc agatgctgca ttatgtgctg ggcatcacca
     1681 aggcgtatac cacgcgcgtg ggctccggcc cgtttcctac cgaattgagt gatgaagtgg
     1741 gcaagaagct cgcgtcacgc ggccaggaat tcggcgcgac caccggacgt ccgcgccgtt
     1801 gcggctggtt cgatgccgct gcattgaagc gctcgatcca gatcaacggc gtctcaggtt
     1861 tgggtgtcac caagcttgat gtgctcgacg gcatgcggag cctgtgcctg ggagtggggt
     1921 ataaaattga gggcgtggat agcgatattc tgccagtggg cgcggaactg ctcgccgaga
     1981 gcgagccggt gtacgaagaa attccaggat ggctagaaag tactgtgggt gtgaagcgtt
     2041 ttgaagacct gccgcgtccg gcgcagaatt atctcaagcg catcgaggaa atttgcgatg
     2101 ttccgattga catgatttcc accggggcgg agcgcgaaga aaccatcgtg ttgcgccacc
     2161 cgttcgcctg atggcagtcg cgaatttcgg gttgtgggtt tcgagttcag atcacgcagt
     2221 ttcaggtttt aacacgaaac cgaaaaccca taactcggaa ccgtatttta ttggtgccga
     2281 gaagaggact cgaacctcca cagtgttgcc accgccagga cctgaacctg gtgcgtctac
     2341 caattccgcc atctcggctt tcgagaaggt gcaaatctaa taggaaaaca ttttcttgtc
     2401 aacgaaatcc cccaagcgtt ttaaatcttt gaccaaagcg gaacgcagcc gctcctctcc
     2461 agcaaagcca aagctgcgcg agcttgatcc gcatttcaag cgcgaacgct cgaaataccg
     2521 ggaagccttg ccgagccgcg aattcatttt acagattctc gcagagcagg gtgttccgct
     2581 gtccgtggaa aaactgatgc aatcgctcga ggtcgccgac catgaggttg cgctgttcca
     2641 gcgccggctg cgggcgatgg agcgcgacgg gcaaatcatg cgcaatcgca aaggcgcgat
     2701 atgcgtggtg gacaagctcg acctcgtcaa gggcacagtg cagggacatc ccgacggttt
     2761 cggttttttg ataccggacg aaggcgggcc ggatctgttt ctcggcccca aggaaatgca
     2821 aaaagtgctg cacggcgacc gggtcatggc gcgtgaaatt ggaaccgacc gccgcggccg
     2881 gcccgaagcc agcatcgtcg aggtgctcac gcgcgccaac cagcgcctgg taggcaggct
     2941 gcacgtagag cacggcatcc agttcgtggt ggctgaaaac aagcgcatca gccaggatat
     3001 tttagtgccg cagggcgaaa gccacgcggc gaagccgggg caggtagtga tggcggaaat
     3061 catcgagcag ccgagcaagc acgagcagcc cgtcggccgc gtagtcgaag tacttggcaa
     3121 ttacgccgac ccgggaatgg aaatcgagat cgcgttgcgc aagcatgatt tgccgcacgt
     3181 gtttccgccg gatgcggaga tatcggcgaa acacctgccg agcgaggtgc tggaaaaaga
     3241 atatgccggg cgccaggacc tgcgcggtct tccgctggtg accatagacg gtgaaaacgc
     3301 aaaggatttt gacgatgcgg tgtactgcga agcgcagggc aaaggattca agttattcgt
     3361 ggcgattgcc gatgtcagtc attatgtgaa gcccggagac gccctggatc gggaagcgtt
     3421 gcaacgcggc aactcggtgt attttccgcg gcgcgtgatt ccaatgctgc ctgaagcttt
     3481 gagcaatgat ctttgctcgc tcaacccgag agttgcgcgc ctgacaatgg tgtgcgaaat
     3541 gcaaatcgcc gctgacggcg aaatcggcga ataccgtttt tatccggcgg tgatgctctc
     3601 gcaggcgcga ttaacctaca ccgcggttgc tgagatgctc gagcatcctg aaggcgatac
     3661 tgcgcgccgc ttcggcaggc tgctgccgca tattaaaata ctttacgcgc tctacaaggt
     3721 cttggccaaa gccagggaaa aacgtggcgc gattgacttc gagactatcg aaacgcagat
     3781 gattttcaat gagcatggaa aaatcgagcg catcgttccc gtgaagcgca acgacgcgca
     3841 ccgcttgatt gaagaatgca tgctggctgc caacgtctgt gcctcggatt tcctcaagag
     3901 ccgcgggcag cgcacgctat accggattca cgaaggcccg accccggaaa agctcgacac
     3961 tttacatggt tttctcaggg aattcgcttt gcatttgggc ggaggcgaca atccgcatgc
     4021 caaggattac gccagactgt tgagcaccga ccgcgaccgt cctgatgcac aattgctgca
     4081 aacggtgatg ttgcgttctc tgcaacaagc tatgtacagc ccggacaacg tgg
//
LOCUS       DATIOW010000006         2327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1628525, whole genome shotgun sequence.
ACCESSION   DATIOW010000006 DATIOW010000000
VERSION     DATIOW010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1628525"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1912
                     /locus_tag="VLL03_00115"
     CDS             <1..1912
                     /locus_tag="VLL03_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS45810.1"
                     /translation="SDFLMALQLTGAAKADSIANMQLRLPVVVIGGGLTAIDTATESL
                     AYYPLQVEKFLHRYETLVAERGVAAVRRNWHPEERLIADEFLEHAKAIRAERAAAQAE
                     GRKPRILELLQSWGGVTIAYRKRLIDSPSYTLNHEEVEKALQEGIKFAEGLNPLRVEV
                     DSFGAARAIRMSVQSRDAAGHWSEVNQVELTAHTILVAAGTQPNTVLAREDPGNFKLD
                     GRYFQACDEDGNPVKPERNISKPKAAHVLLSKRSDGRFISFFGDLHPSYYGNVVKAMG
                     GAKQGYPVVSGVLQRVKAASDKSDAEFFAHLNNELRATVRDVIRLTPSIVEVVVKAPQ
                     AARRFRPGQFYRLQNYETHAIKVDGTQLTMEGLALTGAWVDTKKGLISTIVLEMGGSA
                     DLCTLLEPGEPVVLMGPTGSPTEIPSGETVILAGGGLGNAVLFSIGQAMRRTGSKVLY
                     FAGYKKMIDRYKVEQIEAAADVVVWCCDEHPGFQPDRTQDRAFVGNIVQGITAYARGA
                     LGPQQIAVRNADRIITIGSDKMMAAVAEARHAVLKPFLKPHHFAIGSINSPMQCMMKE
                     ICSQCLQPHVNPETGEITYVFSCFNQDQPLDQVDFEGLSARLSQNSLQEKLTAQWIDH
                     CLIRLQRRQAAA"
     gene            2054..>2327
                     /locus_tag="VLL03_00120"
     CDS             2054..>2327
                     /locus_tag="VLL03_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HSS45811.1"
                     /translation="MTLVEMIDALLPQTQCGKCGYGGCKPYAEAIAAGEAEINQCPPG
                     GERGIHRLAVLLNREFKPLNPQFGVEKPREVAVIDEQTCIGCTLCIE"
BASE COUNT          550 a          654 c          665 g          458 t
ORIGIN      
        1 ttccgatttc ctgatggcgc tgcaattgac cggcgcggca aaagcggact cgattgccaa
       61 catgcagctc agactgccgg tggtggtaat cggcggcggc ttgaccgcaa tagacaccgc
      121 gaccgaatca cttgcctatt atccgttgca ggtcgagaaa ttcctgcacc gctatgaaac
      181 cttggttgcc gagcgtggcg tggcagcggt gcgccgcaac tggcaccccg aagaacggct
      241 tatcgccgat gagtttctcg agcacgctaa agccatccgc gccgagcgcg cagccgctca
      301 ggccgaaggc cgtaagccgc gcatcctgga attgctgcaa agctggggcg gcgtgactat
      361 tgcctaccgc aagcgcctga tagacagtcc ctcgtatacc ctgaaccacg aagaagtgga
      421 aaaagcgctg caggagggca ttaaattcgc cgaaggcttg aacccgctgc gcgtggaagt
      481 ggattcattt ggcgcggcgc gcgccatccg catgtcggtg cagagcagag acgccgcagg
      541 ccactggagc gaagtcaatc aagtcgaact taccgcacac accattttgg tcgcggcggg
      601 aacacagccc aacactgttc tggcgcgcga ggaccccggg aatttcaaac tggacggccg
      661 ctacttccag gcctgtgacg aagatggcaa tccggtaaaa cccgagcgca atatttccaa
      721 gcccaaggcc gcgcacgtgc ttttgtcgaa gcgcagcgac gggcggttta tcagcttctt
      781 tggagatctg catccttcat attacggcaa tgtagtgaaa gcgatgggcg gggcaaagca
      841 aggttatccg gtggtgagcg gtgtgctgca gcgcgtaaaa gcggccagcg acaaaagcga
      901 cgcagaattc tttgcgcacc tcaataatga actgcgtgcc accgtgcgcg atgtaatccg
      961 tcttacgcct agcatcgtcg aagtcgttgt caaagcgcca caggcggcgc gccgcttccg
     1021 tcccggtcag ttctaccggc tgcagaatta cgaaacccac gcgattaaag tggacggcac
     1081 gcagctcact atggaaggtc ttgcgctcac cggcgcgtgg gtggatacca aaaaaggttt
     1141 gatttccacc atcgttctgg aaatgggcgg atcggcggat ctgtgcacgc tgctcgaacc
     1201 cggagaaccg gtggttctga tggggccgac cggttcaccc actgagattc cttcgggtga
     1261 gacagtaatt ctagccgggg gcggcctggg taatgcagtg cttttctcta tcggccaggc
     1321 aatgcgccgc accggttcca aagtactgta tttcgccggc tacaagaaaa tgatagaccg
     1381 ctacaaggtg gaacaaatcg aagccgccgc agacgtggtg gtgtggtgct gcgacgaaca
     1441 tcccggtttt caaccagacc ggacgcagga ccgcgctttt gtcggcaaca tcgtgcaggg
     1501 catcaccgcc tacgccagag gcgcgctggg gccgcagcag attgccgtac gtaacgcaga
     1561 ccgcattata accatcggct cagacaagat gatggcggcg gttgccgaag cgcggcatgc
     1621 cgtgctgaag ccttttctca agccgcacca tttcgccatc ggctcgatca actcgccgat
     1681 gcagtgcatg atgaaggaaa tctgttcgca gtgcctgcag ccgcatgtta atcctgaaac
     1741 cggcgagatt acttatgtat tctcctgctt caaccaggac caaccgctcg accaagtgga
     1801 tttcgagggg ctctccgcgc gcctgagcca aaattcgctg caggagaaac tcactgcaca
     1861 gtggattgac cactgcctca tccgactgca acgccgccag gccgcagcct gaccaaaaag
     1921 caaaatcgaa aagacaggat tcacagaatt ttgcaagatt aacaggatta atcttgtaaa
     1981 tcctgaagaa tcctgttaat cctgtcaatt tcaaggcttc ctctcgcgtc cttgcagctt
     2041 aggattgaaa atcatgacgc tggttgaaat gattgatgcg ctgttgccgc aaacccaatg
     2101 tggcaagtgc ggctatggcg gctgcaagcc atacgccgaa gcgattgccg caggcgaagc
     2161 cgaaatcaat caatgtccgc ccggcggtga gagaggaatt cacaggcttg ctgtgctatt
     2221 gaaccgagaa ttcaagccgc tcaatcccca attcggcgtg gaaaaaccaa gggaagttgc
     2281 ggttatcgac gagcaaacct gcataggctg cacgctgtgc atcgaag
//
LOCUS       DATIOW010000007         5138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_511252, whole genome shotgun sequence.
ACCESSION   DATIOW010000007 DATIOW010000000
VERSION     DATIOW010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511252"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1746
                     /locus_tag="VLL03_00125"
     CDS             <1..1746
                     /locus_tag="VLL03_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HSS45812.1"
                     /translation="RDTAAELLNLYAQRASRQGHAFSFQPHDYEAFVDGFSFEETPDQ
                     AAAIQSVIEDLTQGKPMDRVICGDVGFGKTEVALRAAFIAMLDGKQVAVLVPTTLLVE
                     QHFQNFSDRFADWPVKIAELSRFRSGKEQTQALEGLADGRIDIVIGTHKLLQKGVKFK
                     RLGLVIIDEEHRFGVRQKERLKALRAEVDVLTLTATPIPRTLAMSLEGLRDFSVIATA
                     PERRLAIKTFVTPYSQGIIREAALRELKRGGQVYFLHNEIDTIETMYEKLTKLLPEAR
                     IHFAHGQMRERDLEHVMRDFYQQRFNMLVCTTIIETGIDIPTANTMIINRADKFGLAQ
                     LHQLRGRVGRSHHQAYAYLLTPDEEAVGAQARKRLEAIQMMEELGSGFYLAMHDLEIR
                     GAGEVLGEAQSGEMQEVGFNLYNQMLGAAVISLKNGKEPDLSQPLGVTTEINLHVPAL
                     LPEHYCNDIHERLVLYKRLANCETLEELELMQEELVDRFGSPPQPAKVLLDTHRLRIL
                     GKPLGISRIDATAEHLTLQFEPKPPVDAAKIIQLMQQKRNYRLSGPDRLRIEAELPDV
                     KARVAAIREAFSELS"
     gene            2552..3382
                     /gene="serB"
                     /locus_tag="VLL03_00130"
     CDS             2552..3382
                     /gene="serB"
                     /locus_tag="VLL03_00130"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HSS45813.1"
                     /translation="MNLIIQGSEIENRDLRQLAKLAGASRIEQIMHEAFRLREAQARD
                     GVAEYCEKAKLDFAFVPENLKLSSFGLLAMDMDSTLITIECVDELADMQGIKAEVSQI
                     TASSVRGEIEFTESMRRRVALLAGLDESALQRVYDERLQLSPGAEKMLAGLKVAGVKT
                     LLISSGFTFFTDRLKKRLNLDYTCSNTLEIVNGKLSGKVAGEIIDAETKTVKVEEIRK
                     QLGLKKEQVIAVGDGANDLKMMAQAGVSIAYHAQPIVRRQTTYAINHVGLDGVTNLFA
                     "
     gene            complement(3387..3854)
                     /locus_tag="VLL03_00135"
     CDS             complement(3387..3854)
                     /locus_tag="VLL03_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HSS45814.1"
                     /translation="MIKIISGGQTGVDRAALDAALALGTPCGGWCPEGRLAEDGRIPE
                     RYPLKELAGAGYVERTLQNVIDSDGTVIINFGELQGGTEQTLLFCVEQAKPHELINAE
                     EVPALKAAKLIGVFVRKHRIEILNVAGPRHSKEARAYAYALEAVRRFLVELQT"
     gene            complement(3876..4292)
                     /locus_tag="VLL03_00140"
     CDS             complement(3876..4292)
                     /locus_tag="VLL03_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSS45815.1"
                     /translation="MIKRILMAYDGSAPADKAYAFAMDLAKKYGAELIVLAVSRPPDF
                     ADDVEMEAVLENSTKHYEKLLSNLKPQAAAQGINARFEVVVGHPAEQILYYAEQNEAD
                     HIVMGHRGKTFFQRWRLGSVSKQVIHYALCAVTVIR"
     gene            complement(4305..>5138)
                     /locus_tag="VLL03_00145"
     CDS             complement(4305..>5138)
                     /locus_tag="VLL03_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSS45816.1"
                     /translation="ALSTTSVAVVYAVMLEFGFNKTDYGKTVLAACFVTDLGTVLALG
                     FIFAPFTFKTLIFFGVTVVVLAILPWLTSRLFKRYGERPSELEAKYLLLCLFGLGALA
                     AWADSEAVLPAYVIGMVLAGTVGKDHALIRRLRTLTFGLLTPFYFIRAGSFVSVPALL
                     AAPAAFIILLLAKMATKFAGVYPVTKFYGSPRTEAMYTTLLMSTGLTFGSISALFGLS
                     HHIIDQSQYSLLVVTVIGSAVVPTLIANAFFLPRYLLKKVPEEERWAAGQEIPRLADP
                     E"
BASE COUNT         1224 a         1464 c         1441 g         1009 t
ORIGIN      
        1 cgcgacaccg ccgccgagct tttgaatctc tacgcgcagc gtgcgtcgcg ccagggccac
       61 gctttcagct tccagccgca cgattacgaa gcgttcgttg acggtttcag tttcgaggaa
      121 acacccgatc aagcggcggc catccagtcc gtaatcgagg atctaactca gggcaaaccg
      181 atggaccgtg tgatttgcgg cgacgtcggt ttcggcaaga ccgaggtcgc gctgcgcgcg
      241 gcgttcattg ccatgctgga cggcaagcag gtggcggtgc tggtaccgac cacgcttctc
      301 gtcgagcagc atttccagaa tttctccgac cgcttcgccg actggccggt gaaaatcgct
      361 gagctgtcgc gcttccgctc gggcaaggaa cagacgcagg cgctagaagg tctggccgat
      421 ggcaggatcg acatcgtcat cggcactcac aagcttttgc agaaaggcgt caagttcaag
      481 cgtctcgggc tggtgatcat agacgaagag caccgcttcg gcgtgcggca gaaggagcgg
      541 ctgaaagcgc tgcgcgcgga ggtagacgtg ctgacgctca ccgccacgcc gatcccgcgc
      601 acgctggcga tgtcgctcga aggcctgcgc gatttctcgg taatcgccac cgcgcccgag
      661 cgcaggctgg cgattaaaac tttcgttacg ccttacagcc aaggcatcat ccgcgaggcg
      721 gcgctgcggg agttgaagcg cggcggtcag gtgtatttcc tgcacaacga aatcgacacg
      781 attgaaacca tgtacgagaa actgacgaag ctcttgccgg aagcacgcat ccacttcgcc
      841 cacgggcaga tgcgcgagcg cgatttggaa cacgtgatgc gcgatttcta tcagcagcgc
      901 ttcaacatgc tcgtctgcac cacaattatc gaaacaggga ttgatatccc caccgccaat
      961 actatgatca tcaaccgcgc cgataaattt ggcttggcgc aactgcacca gctgcgtggg
     1021 cgcgtcggcc gctcgcacca ccaggcgtat gcctatctcc tcactccgga cgaagaagcg
     1081 gttggcgcac aggcgagaaa gcggctggaa gcgattcaga tgatggagga gctgggctca
     1141 ggtttttacc tagcgatgca tgatttggag atacgcggcg cgggagaagt tttaggtgaa
     1201 gcgcaaagcg gagaaatgca ggaagtgggt ttcaatctct acaaccagat gttgggcgcc
     1261 gcagtgatat cgctcaagaa cggaaaagag ccggatctct cgcaacctct gggtgtgacc
     1321 accgaaatta atctgcatgt gccggcgctg ctgccagagc attactgcaa cgatatccat
     1381 gagcgcctgg tgctgtacaa gcgtcttgcc aactgcgaaa cgctggagga actggaattg
     1441 atgcaggagg aactggtgga ccgcttcggc tcgccgccgc aaccggccaa ggtcctgctc
     1501 gacacccacc gcctgcgcat cctaggcaaa ccgctcggca tttcgcgcat tgacgccacc
     1561 gccgaacacc tcacgctgca attcgagccc aagccgccgg tggacgccgc caaaatcatc
     1621 cagctcatgc aacagaagcg caattaccgg cttagtggcc cggaccgcct gcgcatcgaa
     1681 gccgagctac cggacgtcaa ggcacgcgtt gcggcaatca gagaagcgtt cagcgaattg
     1741 agctgatgat cacagaagag gttgacgaca agactaggaa aaccaagtta tgataattat
     1801 aacctggggg tgctgtgtta aaagtcgtcg cacccatttc tgggtgtaac ctggtagcgc
     1861 agggggatat tagattgcat ttatcaggtc ctctgttagg gagtattatt tgaatttcga
     1921 cctaaaaagg gaaactttat gtcgcacaaa cacaaaattg cattactact tacttcttca
     1981 ttagtttgcc cctcattgag caacgttgca tcagcccaag aggagaccca agcaaatgca
     2041 ccagtcttga cccggaaacc ttgcgaagag ctgaagtctg agatcgaggc gaagcttcaa
     2101 gctaagggag ttgaggctta tactctggaa atcgttccga atgaggatgt gaaagacgag
     2161 aaagtagtcg gtagttgcga tggcggaacc aagaagatta cgtaccagag aggctaaggc
     2221 tcttgtaaag gttacgaacc tagcaaccgg ttggagccca ccgactgtgt cgggcgctca
     2281 accggatcgt tggacatcat aatcatcacc atgccctaaa cgatcccggc aaccggtgtg
     2341 gaaccggagg taggtagtgg gtcagttaga agccgttcgt ggtgatgcta agcttgtcga
     2401 agcatcgaac catgaatggc cgtttccccc ttcgacaagc tcagggcgaa cggcctattc
     2461 tgatcaaact aacccactac ccaaaggcgc accgggtcgc agaggacagc cgcctccact
     2521 ataatcgcgc ctttgctcta cggctcacgt catgaatctc atcattcagg gcagcgagat
     2581 cgaaaaccgc gatctccggc aattggcgaa gcttgccggc gcatcacgta tcgagcaaat
     2641 catgcatgaa gcgtttcgtt tgcgtgaagc acaggcgcgc gacggagtgg ccgaatactg
     2701 cgagaaggcg aagcttgatt tcgctttcgt gcccgaaaac ctcaagctct ccagcttcgg
     2761 tctgctggcg atggacatgg actcgacgct gattaccatc gaatgcgttg acgaactcgc
     2821 cgacatgcag ggcatcaaag cggaagtctc gcaaatcacc gcaagctcgg tgcgcggtga
     2881 aatcgagttc acggaaagca tgcgccgccg cgtcgccctg ctcgccgggc tcgatgaatc
     2941 cgccctgcaa cgggtttacg acgagcgctt gcagctttcc cccggcgcag aaaaaatgct
     3001 cgccggactg aaagtggccg gcgtcaagac actattgatc tccagcggct tcactttctt
     3061 caccgaccgg ctgaaaaagc gcctgaatct cgattacacc tgctccaaca cgctggaaat
     3121 cgtcaacggc aaactgagcg gcaaggtggc gggggaaatc attgacgccg aaaccaagac
     3181 agtcaaagtg gaggagatcc gcaagcagct cggattgaag aaagaacagg tcattgccgt
     3241 cggcgacggc gccaacgatc tcaaaatgat ggcgcaggcc ggcgtcagca tcgcttacca
     3301 cgcccaaccc atcgtccgcc ggcaaacgac ttacgccatc aaccacgtcg gtctggacgg
     3361 cgtgactaac ctatttgcct gattgactag gtttgcagct ccaccagaaa acgcctcacc
     3421 gcttccagag cataggcata agcccgcgct tccttgctgt gccgcggccc agcgacattg
     3481 agaatctcga tgcggtgttt cctgacgaac acgccgatta atttggccgc ctttaacgcg
     3541 ggaacttcct ccgcatttat caattcatgc ggcttagcct gttcgacaca gaacaacaaa
     3601 gtctgctcgg tccctccctg caattcgccg aagttaatga tcacggtgcc gtcgctgtca
     3661 ataacgtttt gcagcgtgcg ctcaacataa cccgcccctg caagctcttt aagcgggtaa
     3721 cgctcgggaa tgcgaccgtc ttcggccagc cggccttcag ggcaccagcc gccgcagggg
     3781 gtaccgagcg caagcgcggc atccagcgcg gcgcggtcca ctccggtctg gccgccggaa
     3841 atgattttaa tcattcttgc ggcttttcgg ccgattcagc ggatgacggt tacggcgcaa
     3901 agcgcgtaat gaattacctg ttttgacacc gagcccagcc gccaacgctg gaaaaacgtc
     3961 ttgccgcgat gtcccatgac aatatgatcg gcttcgtttt gttcggcata gtacaaaatt
     4021 tgctctgcgg ggtgccccac caccacttcg aatcttgcat tgatgccctg cgccgcagcc
     4081 tgcggtttca gatttgacag caatttctcg taatgtttgg tggaattctc cagaaccgct
     4141 tccatttcca cgtcatcggc aaaatccggc gggcgggaaa ccgccaacac aatgagttcg
     4201 gctccatact tcttggcgag atccatggca aaagcatatg ccttgtccgc gggcgccgag
     4261 ccgtcataag ccatcaggat gcgcttgatc atggttgtct ccctctattc tgggtcggcg
     4321 agtcgcggga tttcctgccc cgctgcccac ctctcttcct cgggtacttt tttcagcaag
     4381 tagcgcggta ggaagaaagc gttggcgatg agcgtgggca ctaccgcgct gccgatgacg
     4441 gtgaccacca gcagggaata ctgggactga tcgatgatgt ggtgagaaag cccgaacaaa
     4501 gccgagatgc tgccgaaagt aagcccggtg gacattaaca gcgtcgtgta catagcttcc
     4561 gtcctcggcg agccgtaaaa tttggtaacg gggtagacac cggcaaattt ggtcgccatc
     4621 tttgccaaca gtaaaatgat aaacgcggcc ggcgcggcaa gcaatgcggg caccgagacg
     4681 aaggagccgg cacggatgaa ataaaacggc gtgagcaagc cgaaagtcaa ggtgcgcagc
     4741 cgccggatca aagcgtggtc cttgcccacc gtcccggcca gcaccatgcc gattacatag
     4801 gccggcagta ccgcttcact gtccgcccag gcggcgagcg cacccaggcc gaaaagacag
     4861 agcagcagat attttgcttc cagctccgac ggcctctcgc cgtagcgttt aaaaagacga
     4921 gaagtgagcc agggcagtat cgccagaaca acgacggtca cgccgaaaaa aatgagggtt
     4981 ttaaaggtga aaggcgcaaa gatgaagccc agcgccagca ccgttcccag atcggtgaca
     5041 aagcatgctg cgagcacggt cttgccgtaa tctgtcttat taaagccgaa ttccagcatt
     5101 accgcataaa ccaccgcgac cgaggtggtg gaaagcgc
//
LOCUS       DATIOW010000008         3799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1222577, whole genome shotgun sequence.
ACCESSION   DATIOW010000008 DATIOW010000000
VERSION     DATIOW010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222577"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(278..490)
                     /locus_tag="VLL03_00150"
     CDS             complement(278..490)
                     /locus_tag="VLL03_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS45817.1"
                     /translation="MFSLWLVGAEAPLLGTGIGRFLVKTLAARYARPYLDFSDLVESS
                     LNDRQWIDACAPAVAVALLALREKTD"
     gene            complement(506..1324)
                     /locus_tag="VLL03_00155"
     CDS             complement(506..1324)
                     /locus_tag="VLL03_00155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HSS45818.1"
                     /translation="MPDYTVGWDLGGAHLKVALVDGAGRVCKAMQLPCPLWQGLQHLE
                     SAVAQVLGELNQPAALHALTMTGELADIFPNRSAGVTMLVEAMNSKLPHASLQVYAGR
                     SGFVTPAQAKQLAGEVASANWLATAELAAKRVRQALLIDIGSTTTDLVLLNSGKVHAR
                     GWNDHQRMAFDELIYSGVVRTPVMAVVSRVPFAGEWQGLMAEQFATMADVYRLTEELP
                     EGADQLPAADGGGKTMPDSARRLARMLGRDLELAPLSIWQQLASYIAEQQLQRL"
     gene            complement(1317..2318)
                     /locus_tag="VLL03_00160"
     CDS             complement(1317..2318)
                     /locus_tag="VLL03_00160"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HSS45819.1"
                     /translation="MRIFVYEHITGGGLLDQALPARLAREGGVMLTALLRDLADIPEV
                     EIITSRDPRLPALDQPAKVLVPQNAEEAALVFRDGAEMADAVWPIAPETGGVLEHLSR
                     SVLEQDKILLGSRPEAVRLAASKLATSRQLARHGISAVPTYAATDRISDTAEGWVVKP
                     DDGAGCGDTHWFSTLEATRHWLSRTTGAEYYVLQPFIPGKALSLSLLCRAGQAFLLSC
                     NNQRVVVRDGQFHFLGSVVNAMIDGDGAFAELARRVAAAIPGLWGYVGIDLVQSETGA
                     VVMEVNPRLTTSYAGLREALGCNPAALVLGLLDDAKPLRLPKFTPQRIEVDLESLHA"
     gene            2437..3168
                     /locus_tag="VLL03_00165"
     CDS             2437..3168
                     /locus_tag="VLL03_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HSS45820.1"
                     /translation="MKIIPVLDLMHGRVVRADRGERHSYRPIESRLCKGSNALEIASA
                     LLQLHPFTTLYIADIDAIEGNSNNLAIIENLHARFSALELWVDAGIADLAVLAAWQKR
                     KLGHAVIGSESKPDMELLRRLGQDGKAPYPLLSLDFKGSRFRGDEQLLNRTELWFEHV
                     IAMTLSRVGSGAGPDLALLRSLKRRAPQKKFYAAGGVRDARDFELLRQAGASGVLLAS
                     ALHLRRLTPSDLAAWASVTEATGTP"
     gene            complement(3200..3709)
                     /gene="fae"
                     /locus_tag="VLL03_00170"
     CDS             complement(3200..3709)
                     /gene="fae"
                     /locus_tag="VLL03_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HSS45821.1"
                     /translation="MAKIDRVLVGESLVGDGNEVAHVDLIMGPRGSAVETAFCHALTN
                     NKDGFTSLLALVAPNLQAKPNTILFNKVTIKGAKQAVQMFGPAQRAVAMAVADCVQDG
                     TIPANEADDVFMCVGVFIHWLAENDTKIYDYNYQATKESIKRAVAREPKASEVVAKKG
                     AMKHPFSPK"
BASE COUNT          776 a         1220 c         1064 g          739 t
ORIGIN      
        1 tggcgtattt cggtcacttt gccgctcgtg accagcgcgg catagggctc cgccaccaga
       61 atatcgtagc gcgattgcga gccgaaaacc tgccgctgtc gagaaacgcc gcccacggca
      121 aatcggccac cgcctcgaac agtcgggcgc tgtcttcgtg ataaggaagt tctgtgagca
      181 aaggggttgt catgaattaa attctatcac cgcagagacg ccgagacgcg gagcaaatca
      241 attcttcaat aaaaacatcg tctctgcgcc tctgcggtta atcagttttt tcccgcagcg
      301 ccagcagcgc caccgccacc gcaggagcgc aggcgtctat ccattggcga tcgttcaacg
      361 acgattccac caggtcggaa aaatccagat aaggccgtgc gtagcgcgcg gcaagcgttt
      421 ttaccaaaaa gcggccgatt cctgttccga gaagcggcgc ctcagcgccg accagccaca
      481 gcgaaaacaa gcgcgagcag gcttgttaca ggcgctgcaa ttgctgctcg gcgatatagc
      541 ttgcaagctg ctgccagatt gagagcggcg ccaactccag atccctgccg agcatgcgcg
      601 ccaggcgccg cgcgctgtcc ggcatggtct tgcctccgcc atccgcggcg ggcaactggt
      661 ctgccccttc cggcaattct tcggtcaggc gatagacatc cgccatggtc gcgaactgtt
      721 ccgccatcag cccctgccat tcgcctgcga acggcacgcg ggagaccacc gccatcaccg
      781 gcgtgcgcac cactccgcta tagatgagct cgtcaaacgc catgcgctga tggtcattcc
      841 agcctcgggc gtgcactttg ccggaattca gcaacaccag atcggtggtg gtgctgccga
      901 tgtctatcaa taacgcttgc ctcacccgct ttgccgccaa ctcggcggtt gcgagccagt
      961 tcgctgaagc cacctcgccg gcaagctgct tggcttgtgc aggggtaaca aagccgctgc
     1021 gcccggcata cacttgcaag cttgcatgcg gcaacttgct gttcatcgct tccaccagca
     1081 tggtcacgcc ggcgctgcga ttgggaaaaa tatctgcgag ctcgccggtc atagtgagcg
     1141 cgtgcaacgc tgcgggttgg tttagctctc ccagcacctg agcaacagca ctctccaggt
     1201 gctgcaatcc ttgccacagc ggacagggca attgcatggc tttgcatacg cgcccggcgc
     1261 cgtccaccaa cgccactttg agatgcgcgc ctcccaaatc ccagccgacg gtgtaatcag
     1321 gcatgcaggg attcgaggtc gacttcaatc cgctgcggcg tgaattttgg caggcgcaaa
     1381 ggtttggcgt catccagcag gcccaatacc agtgcggcgg gattgcagcc cagcgcctcg
     1441 cgcaaacccg cataggacgt agtcagccgc gggtttactt ccatcaccac cgcgcccgtc
     1501 tcgctttgca ccagatctat gcccacatag ccccacagcc cgggaatggc tgcggccacg
     1561 cgtcttgcta attcagcaaa agcgccgtct ccatcaatca ttgcattgac cacgctgcca
     1621 agaaaatgaa actggccgtc acgcaccact acgcgctggt tattgcagct caaaagaaaa
     1681 gcctggccgg cgcggcacaa aagggaaaga ctcaacgctt ttccaggaat gaacggttgc
     1741 aacacgtaat actccgcacc ggtcgtccgg gacagccagt gtcgggtcgc ttcaagcgtg
     1801 gaaaaccaat gggtgtcgcc gcaacccgcg ccgtcatcgg gtttcaccac ccagccttcc
     1861 gccgtatcgg aaatgcggtc ggtagcagca taagtgggaa ccgcggaaat tccgtggcga
     1921 gccagctgcc gcgaagtggc aagcttgctt gcggcaagcc gcaccgcttc gggacgactg
     1981 cccagcaaaa ttttgtcctg ctccagcacg ctgcggctta agtgctccag aaccccaccg
     2041 gtttccgggg cgatcggcca caccgcgtcc gccatttccg cgccgtcgcg aaaaaccaaa
     2101 gccgcttctt cggcgttttg cgggaccagg acttttgcgg gttggtcgag cgcgggcagg
     2161 cgcgggtcgc ggctggtgat gatttccact tcgggaatat cggccaggtc gcgcagcagc
     2221 gccgtgagca tcacgccgcc ttcgcgcgcc agccgggcag gcagcgcttg gtcaagcagg
     2281 ccgccaccgg taatatgctc gtatacgaat atccgcatta gaaacggact atctcaccgc
     2341 agaggcgcag agacgcggag aaaacccttt gaaaccggac tcaaagctaa tcatgccctt
     2401 ctctgcgcct ccgcgtctca gcggttcaaa tatggtatga aaattattcc agttctagat
     2461 ttaatgcacg gccgggtggt gcgcgccgat cgcggcgagc gccacagcta ccgtccgatt
     2521 gaatcacggt tatgcaaagg cagcaacgcg ctcgaaatcg cttctgcact gctgcaactc
     2581 catcccttca ctacgctata catcgccgac atcgacgcga tagaaggcaa cagcaacaat
     2641 ctggcgatca tcgagaattt gcacgcgcgc ttctcggcgc tcgaactatg ggttgacgcc
     2701 ggcattgccg accttgcagt cctcgccgcg tggcaaaaaa gaaaactggg ccatgccgtc
     2761 atcggcagcg aatccaagcc cgatatggaa ttgttgcgcc gcctcggcca agacggcaag
     2821 gcgccgtacc cgctgctgtc gctggatttc aagggttcac gtttccgcgg cgacgaacaa
     2881 ctcctcaacc ggactgaact ctggtttgag cacgtcatcg ccatgacgct gtcccgcgtg
     2941 ggcagcggtg cagggccgga tctcgcgttg ctgcgttcgc tcaagcgccg cgccccgcaa
     3001 aaaaaattct acgccgcggg cggcgtgcgt gatgcacgcg actttgaact cctaaggcaa
     3061 gcaggcgcaa gcggcgtgct gctggcgagc gcgctgcatc tgcgccgcct cacgcctagc
     3121 gatcttgccg catgggcttc cgtcaccgaa gcaacgggca caccatgagc ggcttaccca
     3181 cgcctgcccg taccccgact tatttcgggg aaaacggatg cttcatcgcg cctttcttgg
     3241 ccacgacctc cgacgccttg ggctcgcggg caactgcgcg cttgatggac tccttggtgg
     3301 cctggtagtt gtagtcgtaa atcttggtgt cgttttccgc cagccagtgg atgaacacgc
     3361 cgacgcacat gaacacatcg tccgcttcgt tcgccggaat ggtgccgtcc tgcacgcaat
     3421 ccgccaccgc catcgccacc gcgcgctgcg ccgggccgaa catctgcact gcttgtttgg
     3481 cgcccttaat ggtcaccttg ttgaacagaa tggtattcgg cttggcctgc aggttcggcg
     3541 ccaccaacgc gagcaaggaa gtaaaaccat ccttgttgtt ggtcagcgca tggcagaacg
     3601 cggtttctac ggcgctgccg cgcggtccca tgataaggtc gacgtgggcc acttcattgc
     3661 cgtcgccgac cagtgattct ccaacgagca ctcgatcaat tttcgccatt ttatgtctcc
     3721 ttagtttggc tagttaacgg tgaaacacca ctccgaaacc tcagcggcgg ccgccctcct
     3781 aggcgccaac gtgctgagg
//
LOCUS       DATIOW010000009         2717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_511692, whole genome shotgun sequence.
ACCESSION   DATIOW010000009 DATIOW010000000
VERSION     DATIOW010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511692"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="VLL03_00175"
     CDS             complement(<1..1295)
                     /locus_tag="VLL03_00175"
                     /inference="COORDINATES: protein motif:HMM:NF006549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HSS45822.1"
                     /translation="MPQHVATLIDAGWVIPVEPRDVLPRHSIAISNETIQAVLPTGEA
                     HARFSAKQHVRLENHALIPGLVNLHTHAAMTLMRGLADDLPLMEWLNKHIWPAETKYV
                     SPQFVRDGTELACAEMLRGGITCFNDMYFFPEAAAEAVLTAGMRAAIGIIVIEFPSSY
                     AGDPEDYLNKGLAARDELRPQPLLSFCMAPHAPYTVSDRTFEKLLTYAQQLDLPIHVH
                     VQETQDEIQQSLAQYKMRPLERLHKLGLLGPNFIAVHAVHLTPQEMALLANEACSVAH
                     CPSSNLKLASGFAPVAALLDHGINVGLGSDGAASNNRLDLFGEMRLAAMLAKAAAKQA
                     DALPAYQALQMATLNGAKALGLDGKIGSLAPGKAADITAVDFSGLDLTPCFDPVSHLV
                     YAAGREQVSHVWVNGKLLLDKRQLTTLSTEDISAKTRYW"
     gene            1516..2628
                     /locus_tag="VLL03_00180"
                     /pseudo
     CDS             1516..2628
                     /locus_tag="VLL03_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
BASE COUNT          593 a          761 c          801 g          562 t
ORIGIN      
        1 tgccagtagc gcgttttggc tgagatatct tctgtgctca gcgtggtgag ctgacgcttg
       61 tcgagcaaca attttccgtt cacccagaca tggcttacct gctcgcggcc tgcggcgtat
      121 accaggtgcg acactgggtc gaagcagggc gtaagatcga gcccggagaa atccactgca
      181 gtgatgtccg ccgccttgcc tggggccagc gagccaattt taccgtccag acctagggct
      241 ttggcgccgt tgagtgtagc catttgcaag gcctggtatg cgggcaatgc atcggcctgc
      301 ttggctgcag ccttggcgag catcgcggca aggcgcattt ccccgaatag gtccaggcga
      361 ttgttgctcg ccgcgccgtc gctgcccagc cccacgttga tgccatgatc cagcagcgca
      421 gcaaccgggg caaaaccact ggcgagtttc aggttggatg acgggcaatg tgcgacactg
      481 cacgcctcgt tcgccagcag cgccatttcc tgcggagtca aatgcaccgc atgcacggcg
      541 atgaaattgg ggccgagcaa tcccagctta tgcagccgct cgagcggccg catcttgtat
      601 tgcgccaggc tctgctgaat ttcgtcctgg gtttcctgca catggacatg aatcggcaaa
      661 tcgagttgct gcgcataggt gagcaatttc tcgaaagtcc tgtcgctaac cgtatacggc
      721 gcgtgcggcg ccatgcaaaa cgacagcaat ggctggggac gcagctcatc gcgcgccgcc
      781 agccctttgt tgaggtaatc ctcggggtcg ccggcatagc tgcttggaaa ctcgatgacg
      841 atgatgccga tggcggcgcg catgccggcg gtgagcaccg cttccgcagc cgcttccgga
      901 aagaagtaca tgtcgttgaa acaggtgatg ccgccgcgca gcatctcggc gcaggcaagc
      961 tcggtgccgt cgcgaacgaa ttgcggcgag acatacttgg tttcagccgg ccagatatgt
     1021 ttgttgagcc attccatcaa cggcaggtca tcagccaggc cgcgcatcag cgtcatcgcc
     1081 gcatgggtgt gaagattgac gagtcctgga atgagcgcat ggttttcgag gcgcacatgc
     1141 tgttttgcgc tgaaccgcgc gtgcgcctcg ccagttggca gtaccgcctg tatcgtctcg
     1201 ttgctgatgg ctatggagtg ccgcggcaat acatcccgtg gttcgaccgg gatcacccag
     1261 ccggcatcaa tcagggtagc gacgtgttga ggcatggctt atccataaaa agcaacgcac
     1321 aaatcagcac ttccattgta accgcgaatg aagaactgcg gcgcaaatgg cgttgcagcg
     1381 ccgggtttta ttcgaccggg acgcattttc ggaggttatg cgctacttgg ccttaaccct
     1441 ccgtgcatga taaaatttta aatttaattt gccgtaattc cagcccccag accaactaaa
     1501 aaaacacccc aattcatgga ccaaatcgcc aaagaaacca tcccggtcag catagaagaa
     1561 gagatgcgcc gctcgtatct agattacgcg atgagcgtca ttgtgggacg ggcgctgccg
     1621 gatgttcggg acggcctgaa acccgtgcac cggcgcgtgc tcttcgccat gcatgaagcc
     1681 aataatgtgt ggaaccgccc ctatgtgaag tgcgcgcgca tcgttggtga agtgctcggc
     1741 aagtaccacc cgcatgggga taccgcgacc tacgaagccc tggtgcgcat ggcgcaggat
     1801 ttttccatgc gctacaccct gattgacggg cagggcaact tcggctcggt ggacggcgat
     1861 gcgccagcgg cgtaccgcta taccgaatgc cgcatggagc ggatttcctc ggagatcctg
     1921 gcagacattg acaaggaaac cgtggatttt gcgcccaatt acgacggcag agagcaggaa
     1981 cccgtggttt tgccgaccaa ggtcccgaat ctactggtca atggttcatc cggaatcgct
     2041 gtaggcatgg cgaccaatat tccgccgcac aacctgagcg aagtggtgga cggctgtctt
     2101 tcactacttg aaaacccgca aatcgatatc gaggaattga ttgaaactat tcaggcgccg
     2161 gatttcccca ccgccggcat catttacgga gtttccggtg taagggaagg ctaccgcacc
     2221 gggcgcgggc ggatagtgat ccgcgcacgg acgcatttcg aagagatgga caagggcaat
     2281 cgtcagataa ttatcgttga tgagcttcct taccaggtga acaaagccgc cctgctcatc
     2341 agaatcggcg agctggtgcg cgacaagaaa atcgaaggca tttccgagtt gcgcgatgag
     2401 tcggacaagt caggcatgcg cgtggtgatt gaactcaagc gcggcgagaa ccccgatgtg
     2461 gtgctgaaca acctgtacaa agaaacgcag atgcaggaca ccttcggcat gaacatggtg
     2521 gcgctggtgg ataaccagcc gcgcctgctt aatctcaagc agcttctcga gtctttcctg
     2581 cgccaccgcc gcgaagtggt gatgcggcgc acggtgttcg aattgcgccc gccaatccta
     2641 ctcgctccgg ggcgtgaata tgcttgttta aagcgttgaa gcggtccaca aagaaactgg
     2701 agaaacctgc cggttcc
//
LOCUS       DATIOW010000010         7563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_3635, whole genome shotgun sequence.
ACCESSION   DATIOW010000010 DATIOW010000000
VERSION     DATIOW010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7563
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3635"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="VLL03_00185"
     CDS             complement(<1..165)
                     /locus_tag="VLL03_00185"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878830.1"
                     /note="catalyzes the formation of arginine from
                     (N-L-arginino)succinate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HSS45823.1"
                     /translation="MPKKAKSAAKTWSGRFSEPVGKLTQRYTASVGFDQRLAPFDIEA
                     SLAHARMLNAK"
     gene            240..1253
                     /locus_tag="VLL03_00190"
     CDS             240..1253
                     /locus_tag="VLL03_00190"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HSS45824.1"
                     /translation="MRSINQNFTLRTLPNFRNLGVLLRIVLLVNGACLIAAALKSASV
                     AQTLREFTTMGALAEPVLAVSLLMLMLANPWLGQLNYYLGAGLVLILESAITLLIYYF
                     GIDIYGQGDAGPWRYELFSVLLTASLLIYFDLRNRALSPALSEARLQALQARIRPHFL
                     FNSINAVLSLIRSDTKRAEIALEDLADLYRVLMADSRELSTMRDEVDLCRRYLSLEEL
                     RLGGRLQVEWHIDNMPEDAKIPPLVLQPLLENAVYHGIEPRTEPGVISINIYLSNNQV
                     HAVLRNPYRRDGGHHVGNKMAVQNIRERLSLHFDVEASFTATERSGSYEVHIVIPYLR
                     AEK"
     gene            1250..2005
                     /locus_tag="VLL03_00195"
     CDS             1250..2005
                     /locus_tag="VLL03_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HSS45825.1"
                     /translation="MTGPMRIMIVDDEAPARSRLRELLADCAESFPHVVVADAANGRE
                     ALDLISREPADLLLLDIRMPEMDGIEVARHLLKLEPIPAIIFTTAYDVYAIQAFEVNA
                     VDYLLKPVRGERLLAALQKARQGRPPEQARLQQLARQPRTNLSVQERGKIILVPVADI
                     IYLKAELKYVTIRTAAREYLLEEPLTDLEQEFAARFIRIYRNCLVAKASITVFDKVTE
                     EGSEPYWVVVLKGVEEKLPVSRRQQYLVKEFTR"
     gene            2106..3059
                     /gene="hemC"
                     /locus_tag="VLL03_00200"
     CDS             2106..3059
                     /gene="hemC"
                     /locus_tag="VLL03_00200"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HSS45826.1"
                     /translation="MLSPSPSTTVPAKIVIASRESALAQWQARFIQARLSALYPKLEI
                     RILGMTTQGDTLLDTPLAKIGGKGLFVKELEQALQEGRADIAVHSMKDLPMRLPSGFT
                     LAAITEREDPRDAFVSNRYKSPAELPAGSVVGTSSLRRESQLRACFPHLEVNSLRGNV
                     GTRLKKLDEGRYAAIILAAAGLKRLGLESRITALLSSEQSLPAPGQGALGIECLVSRR
                     DLLELLRPLNHQQTALCVSAERAMSRALSGSCQVPLGGYAKIQNDEIWLRGFVATPDG
                     RRLIRAEKRGNCNQPEALGESLAQELKAKGAAQILAELESA"
     gene            3056..3871
                     /locus_tag="VLL03_00205"
     CDS             3056..3871
                     /locus_tag="VLL03_00205"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HSS45827.1"
                     /translation="MIDVRSKSAPSKSALAGRNIVVTRPAGQAHRLADLIRAAGGRAI
                     LFPALEIRDVADTRPLERLVARLEEFDLAIFVSANAVNRAMSLIAARRTLPAKLKFAT
                     VGAGGLRELQRFGVKEAICPASRFDSEALLELPQLQDVNGKRVVIFRGQGGRELLADT
                     LTERGAKVECAECYRRAKPAADAAAWSELWQKHAPDAVTVTSSEGLRNLFEMAGQKGR
                     NVLIKSNLFVPHPRIAEAARALGLNNVIVTAAGDEGLVQGLIEWFDAETQQSS"
     gene            3868..4917
                     /locus_tag="VLL03_00210"
     CDS             3868..4917
                     /locus_tag="VLL03_00210"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HSS45828.1"
                     /translation="MSENPQPETMPVEATVPDTKALRRLNLALIVALMAAVLMGWLWF
                     DTRGQIAALQQEFAKRLADTIGIVKDNRAIVKDTQEAVRETQVKLGLLESKLAESQNQ
                     QAAMEALYQELSRNRDEWALAEVEQILNIASQQLQLAGNVKAALIALQAADARLQRVD
                     RPQLIPLRKAINRDIQRLKAVPYVDSAAISVRLDNLNAAVDTLPLAMDVRPRSDQSEE
                     AQEATQNAWLKFAREIWQDMKQLVRIQNTVKREIPLLAPNQVFFLRENLKLKLLSARL
                     ALMLHDEVSYKADLASAQEWLTRYYDNSAKVTKVMLGSLRQLQESPISIELPDITASL
                     DAVRKFKLGRDKAAR"
     gene            4914..6140
                     /locus_tag="VLL03_00215"
     CDS             4914..6140
                     /locus_tag="VLL03_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HSS45829.1"
                     /translation="MKTLFWLIAIFALAVGLTLAFGFSNGYVLVVQSPYRIELSLNLA
                     ILLLLGAFIVVYFLVRLAVHTLRLPTQVRAYRLRRRGEKARKSFAEALRHFFEGRYGK
                     AEKAAANALELGESPGLTALLAARAAHEVKAFEKRDEYLAKAENEAPDVATARVIIKA
                     EMLLDQRRPIEALDLLWSLNKDSSKKHAAALRLELRAQQQARNWEQVLQLIAQLEKCG
                     VYDETQARGMRLYATQENLKRQAGDAQALKEYWQKIPSTDKKNLRVAAAAARCFMALG
                     GCRLAHEIIEESLENEWDSDLICLYSECLGQDAETLKQIQRAESWLISHRRDACLLLT
                     LGKLCAHQALWGKAQSYLEASISVEPTPTAHRELAKLYEKTGRNEAANVHYRKSLDLA
                     FRRLGNVTGGRRKAAL"
     gene            complement(6182..7213)
                     /locus_tag="VLL03_00220"
     CDS             complement(6182..7213)
                     /locus_tag="VLL03_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HSS45830.1"
                     /translation="MPFQITIKPSEHVFSVEPGEAILEAALRQGFTLPYGCRNGACGS
                     CKGKILQGTVDYGVYQESALSNVEKHAGMALFCQSKPQSDLVIECREINAVKDIQIRK
                     LPCRLQKMEQPADDVMVLYLKLPANERLQFLAGQYIDILMKDGKRRSFSMANPPHDDE
                     HIQLHVRNIVGGAFTDYVFNKMKEREILRFEGPLGTFFLREDSDKPIIFVASGTGFAP
                     IKGIIEHAFYKSIKREMVLYWGARKLTDLYMQQLPLQWAREHENFSFVPVLSDAIPDD
                     NWQGRTGFVHRAVMEDFTDMSGFQVYACGAPVMVEAARKDFTSLCKLSPEEFNSDAFT
                     YSNDPKPAA"
     gene            7298..>7563
                     /locus_tag="VLL03_00225"
     CDS             7298..>7563
                     /locus_tag="VLL03_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HSS45831.1"
                     /translation="MRRLLIVGCGDIALRMVRQLRGGYRIYALSHSPTRFDLLRTHGI
                     TPIPGDLDKPETFTTLGGLAHDVVHFAPPPSRGTRDMRTAHLLAA"
BASE COUNT         1585 a         2182 c         2269 g         1527 t
ORIGIN      
        1 cttggcattg agcatgcggg cgtgcgccag cgacgcctcg atgtcgaaag gcgcaagccg
       61 ctggtcgaaa ccgaccgacg cggtataacg ttgcgtgagc ttgcctaccg gttcgctgaa
      121 tcttcccgac caggtctttg cggcgctctt ggcttttttg ggcatgacgt gcttcttgtc
      181 aaaagtggtg cactatgtaa ctcttagcag cggtaacggc taaccgtcgc gagctcaaaa
      241 tgcgaagtat aaatcaaaac ttcaccctcc gtaccctgcc gaatttccgc aacctcggcg
      301 tcctgctgcg catcgtgctg ctggtaaacg gcgcgtgtct gattgcggcg gcacttaaaa
      361 gcgcgtccgt cgcgcaaacc ctgcgggaat tcaccaccat gggggcgctg gccgagccgg
      421 tgctggcagt gagcctgttg atgctgatgc tcgccaatcc ctggctgggt caattgaact
      481 attacctcgg cgccgggctg gtgctgattc tggaatcggc gatcactcta ttgatttatt
      541 atttcggcat cgacatttat ggccaggggg acgcaggccc atggcgctac gaattgttct
      601 ccgtcctgtt gacggcgagc ttgcttatct atttcgattt gcgcaaccgc gcgctctcgc
      661 cggcgctgtc cgaagcgcgg ctgcaggcgc ttcaagcgcg catccgtccg catttcctgt
      721 tcaacagcat caacgccgtg ttgagcctga tccgctcgga cacgaagcgc gccgagatcg
      781 cgctggaaga ccttgccgat ttgtaccggg tgctgatggc cgacagccgc gaactttcca
      841 ctatgcgcga cgaagtggat ttgtgccggc gttacttgag cctggaggaa ttgcgcctgg
      901 gcggtcgcct gcaggtggag tggcacattg acaacatgcc ggaagatgca aaaataccgc
      961 cgctggtgtt gcagccgctt ttggagaacg ccgtctatca cggcatcgag ccgagaaccg
     1021 agccgggcgt catcagcatc aatatttatc tgagcaataa ccaggtgcat gccgtgctgc
     1081 gcaatcctta tcggcgcgac ggcggccatc acgtcggcaa caaaatggcg gtgcagaaca
     1141 ttcgcgagcg cttgagcctg cattttgacg tggaggcgag cttcaccgcc acggagcgca
     1201 gcggttccta cgaggtgcac atcgtcattc cctatttgcg cgcagaaaaa tgaccggccc
     1261 catgcgcatc atgatcgtgg acgacgaagc gccggcgcgc agccgcctca gagagctgct
     1321 cgccgattgc gcggaaagct tcccccatgt ggtggtggcg gacgcggcga acggccgcga
     1381 agcgctggat ttgatttcgc gcgagccggc agacttgttg ctgctcgaca tccgcatgcc
     1441 ggaaatggac ggtatcgaag tcgcgcgcca tctgttaaag ctggagccga tacccgccat
     1501 cattttcact accgcctacg atgtctacgc gattcaggcg ttcgaagtca acgcggtgga
     1561 ttatctgctc aagcccgtgc gcggcgaacg gctgctcgcc gctttgcaga aagcgcggca
     1621 gggccgcccg ccggagcagg cacggctgca gcaattggca aggcagccgc gcaccaactt
     1681 aagtgtgcag gagcgcggca aaatcatact ggttccggtt gccgacatca tctatctcaa
     1741 ggccgaattg aaatatgtaa cgatacgcac cgcggcgcgc gagtatttgc tggaggagcc
     1801 gctcaccgac ctggaacagg aattcgcggc acgcttcatc cgcatctacc gtaactgcct
     1861 ggtggcgaag gcaagcatca ccgtgtttga caaagtaacc gaggaaggca gtgagccata
     1921 ctgggtggtg gtgctgaagg gggtggaaga aaaactgccg gtgagtcgcc gccagcagta
     1981 cctcgtcaaa gaattcacac gctagaaacc tctgattaag cccctcgcgt ggcgcgttgc
     2041 gggcgggggg gcatgttaaa atagtcgtgg aaactttctt gagcctcacc cgtgcgccag
     2101 aacatatttt gagcccatct ccctcaacca ccgttcctgc caaaattgtc atcgcctcgc
     2161 gtgaaagcgc gcttgcgcag tggcaggcgc gtttcattca ggcccgactc agtgcgctct
     2221 acccaaaact cgagatcagg attctcggca tgaccacgca aggcgatacg ctgctggata
     2281 cgccgctcgc caaaatcggc ggcaagggtt tgttcgtgaa ggaactggaa caggctttgc
     2341 aggaaggccg cgcggacatt gcggtgcatt cgatgaaaga cctgccgatg cgcctgcctt
     2401 caggttttac tctggcggcc atcaccgagc gtgaggatcc gcgcgatgcg ttcgtgtcga
     2461 atcgttacaa atcgcctgcc gaattgcctg cgggcagcgt ggtcggcacc tccagcctgc
     2521 gcagggaaag ccagctgcgc gcatgctttc cgcatctcga ggtgaattcg ctgcgcggca
     2581 atgtcggcac gcgcctcaag aaactcgatg aagggcgtta tgccgccatt attcttgcgg
     2641 cagccgggct caagcgcctc ggattggaaa gccgcataac cgcattgtta agttccgagc
     2701 aaagcctgcc ggcgccggga cagggcgcgc tgggaattga atgcctggtc tcccgccggg
     2761 acctgcttga gttgctgcgg cccttgaatc accagcaaac cgcgctatgc gtcagcgccg
     2821 agcgcgccat gagccgggcg ctttccggca gttgccaggt gccgctgggc ggttatgcca
     2881 aaatccagaa tgatgaaatc tggctgcgcg gctttgtcgc gaccccggac ggccgccgcc
     2941 tgatccgcgc ggaaaaacgc gggaattgca atcagcccga agcgctgggc gaatcgctgg
     3001 cgcaggaatt aaaagccaaa ggcgcagccc aaatcctcgc cgaactggaa agcgcatgat
     3061 tgatgtccgc tctaaatcgg cgccttcgaa atccgcgttg gcgggacgca atatcgtggt
     3121 gacgcggccg gcggggcagg cacatcggct tgcggattta atccgcgccg ccggcggtcg
     3181 cgctatcctg tttccagcgc tggaaatccg ggacgtggcc gacacacgac cgttggaacg
     3241 gctcgtcgcg cggctcgagg aatttgacct cgcgattttc gtgagcgcca acgcggtgaa
     3301 cagggcgatg agcttgattg ccgcacggcg taccctgccg gcaaagctca aattcgcaac
     3361 cgtcggcgca ggcggcctga gggaattgca gcgctttggc gtgaaagaag cgatttgccc
     3421 cgcgtcgcgc tttgacagcg aggctcttct cgaacttccg cagttgcagg acgtgaacgg
     3481 aaagcgtgtg gtgatttttc gcgggcaggg cggacgcgag cttctcgcgg acacgctcac
     3541 tgagcgcggc gctaaagtcg aatgcgcgga atgctatcgg cgtgccaagc ctgcggcgga
     3601 tgcggcggca tggtcggagt tgtggcaaaa gcatgcgccg gatgcggtca ccgtgaccag
     3661 cagcgaaggt ttgcgcaatc ttttcgagat ggccgggcag aaaggacgga atgtgctgat
     3721 caaatcaaat ctcttcgtgc cgcatccgcg cattgccgaa gcggcgcgcg cgctggggct
     3781 gaataatgtc atcgtaacgg ctgcaggtga tgagggtttg gtgcaaggct tgatcgagtg
     3841 gtttgacgcc gaaacgcagc aaagttcatg agtgaaaatc cgcaacctga aaccatgcca
     3901 gtagaagcga cggtgcccga taccaaagcg ctaaggcgcc tgaacctggc gctcatcgtt
     3961 gcgcttatgg ccgcggtgct tatgggctgg ctgtggtttg acacgcgcgg ccagatcgca
     4021 gcattgcagc aagagttcgc caagcgcttg gctgatacca ttggcatcgt caaggacaac
     4081 cgcgccatcg tcaaggacac gcaggaagcg gtgcgcgaaa cgcaggtgaa gcttggtttg
     4141 ctggaaagca agctcgccga gtcgcaaaac cagcaagccg caatggaagc actgtatcag
     4201 gagctctcgc gcaaccgcga tgaatgggcg ctcgccgaag tcgagcagat actcaacatt
     4261 gcaagccagc aattgcagct cgccggcaac gtcaaggcgg ccttgattgc gctgcaggcg
     4321 gcggatgcgc gattgcagcg cgtcgaccgc ccgcagctca tcccgctgcg caaagccatt
     4381 aacagggaca tccagcgcct gaaagccgtg ccttacgtgg actcggcggc catcagcgtg
     4441 cgcctcgaca atttgaatgc cgctgtcgac acgctgccgc ttgccatgga tgtacgcccg
     4501 cgttcggacc aatccgaaga agcacaggag gctacgcaga acgcgtggct taaattcgcg
     4561 cgcgaaatct ggcaggatat gaaacaactg gtgcgcatcc agaacaccgt caagcgtgaa
     4621 attccgcttc tcgcgcccaa ccaggtgttt ttcctgcgcg agaacttgaa gttgaaactc
     4681 ctgagcgcgc gcctggcgtt gatgctgcac gatgaagtga gctacaaggc cgacctggct
     4741 tccgcgcagg agtggctcac ccgctattac gacaacagcg ccaaagtcac caaggttatg
     4801 ctggggagcc tgcgccaact acaggaaagc cccatcagca tcgaactgcc cgatattacc
     4861 gcgagtctcg atgcggtgcg caagttcaag ctcggtcgcg acaaggcggc acggtgaaaa
     4921 ccctgttctg gctgattgcg atttttgccc tcgcggtggg gctcacgctc gcgtttggct
     4981 tcagcaatgg ctatgtgctg gtggttcaat cgccctatcg catagagctg tcgctcaatt
     5041 tggcgatact gctgctgctt ggcgcgttca tcgtcgttta tttcctggtg cgcctcgccg
     5101 tgcataccct gcgcctgccg acgcaggtgc gtgcctatcg tttgcgccgc cgcggcgaaa
     5161 aagcgcgcaa atcgtttgcg gaggccttga ggcatttttt cgagggccgt tacggcaagg
     5221 cggaaaaagc ggcggccaac gcgctggaac tgggcgaatc gccgggatta acggccctgc
     5281 ttgccgcgcg cgcggcgcat gaagtaaaag cttttgaaaa gcgcgacgag tatctggcca
     5341 aagcggaaaa tgaagcgccc gatgtcgcca cggcgcgcgt gattataaaa gccgaaatgc
     5401 tgctcgacca gcgccgtccc atagaagcgc tggatttgct ttggtcgctc aataaagact
     5461 cctcgaaaaa acacgcagcg gcgctgcggc tcgaacttcg cgcgcagcag caggcgagaa
     5521 actgggaaca ggtgctacaa ctcatcgcgc aactggaaaa gtgcggcgtc tatgatgaaa
     5581 ctcaagcgcg gggaatgcgg ttgtatgcca cgcaggaaaa cctcaagcgt caggcggggg
     5641 atgcgcaggc gctgaaggaa tactggcaga agattccttc aaccgacaag aaaaacctcc
     5701 gcgtcgccgc cgccgccgcg cgctgcttca tggcgctcgg cggctgccgc ctggcgcatg
     5761 aaatcatcga ggaaagtctg gaaaacgaat gggacagcga tttgatttgt ctttattccg
     5821 aatgcctggg tcaggatgcg gaaacactca agcaaatcca gcgcgctgaa agctggctca
     5881 tttcccaccg ccgcgatgcg tgtttgttgc tgaccctcgg caagctgtgc gcgcaccagg
     5941 cgctgtgggg caaggcgcaa agttatcttg aagccagcat cagcgtcgag ccaaccccca
     6001 ccgcgcatcg tgaacttgca aagctgtacg aaaagaccgg ccggaacgaa gccgccaatg
     6061 tgcattaccg caaaagcctg gatttggcat ttcgtcggct aggcaacgtc accggcggta
     6121 ggcgtaaggc agcgctttga ttttttgaac cgccaagacg tatcccggcg cccgtggcgg
     6181 ttcaggcagc cggtttggga tcgttggaat aagtaaacgc gtcggagttg aactcttcgg
     6241 gcgagagctt gcatagtgaa gtgaagtcct tgcgcgcggc ttcgaccatc accggcgcgc
     6301 cgcaggcgta gacttgaaag ccgctcatgt ccgtaaaatc ttccatcacc gcccggtgca
     6361 caaatccggt tctcccctgc caattatcgt ccggaattgc atccgagagc accggaacaa
     6421 aactgaaatt ctcgtgctca cgcgcccact gcagcggcag ttgctgcatg tataaatccg
     6481 tcaatttgcg cgctccccaa tacagcacca tttcccgctt tatgcttttg taaaacgcgt
     6541 gctcgatgat gcccttaatg ggggcgaagc cggtgccgct ggcgacgaag ataatcggct
     6601 tgtcggaatc ctcgcgcaga aaaaacgtgc caagcggccc ttcgaagcgc aaaatttcgc
     6661 gttctttcat tttattgaat acatagtcgg tgaacgcccc gccgacgata ttgcggacgt
     6721 gcaactggat atgctcgtcg tcatgcggcg gattggccat gctgaagctt ctgcgcttgc
     6781 catccttcat gagaatgtca atgtactggc cggcgaggaa ctgcaagcgc tcattcgcgg
     6841 gaagcttgag gtaaagcacc atgacatcgt ccgccggctg ctccattttc tgcaagcggc
     6901 acggcagttt cctgatttga atgtccttga ccgcgttgat ttcacgacat tcgataacca
     6961 aatcgctttg cggtttggac tggcagaata gcgccatacc ggcgtgtttt tccacgttac
     7021 tcagcgcgct ttcctgataa acgccgtaat ccaccgtgcc ctggaggatt ttgcctttgc
     7081 acgagccgca ggcgccgttg cggcagccgt aggggagggt aaagccctgg cgcaaagcgg
     7141 cttcgagtat cgcttcgccg ggctcgacgg aaaagacgtg ttcgctgggc ttgatagtga
     7201 tctggaaggg cattaactca aatccttaag gcgggatttg ccgtctctta tagtggtgac
     7261 ccgccatcat tctaaaattt attctacaat taacagcatg agacggcttt tgattgtcgg
     7321 ctgcggcgat attgccctgc gcatggtgcg tcaactgcgc ggcggctatc gcatctatgc
     7381 gctttcgcat tcgcccacgc gcttcgattt actgcgcacg cacggcatta cgccgattcc
     7441 cggagacctc gataaaccgg agacgttcac cacgctcggc ggcctggcgc atgacgtggt
     7501 gcatttcgct ccgccgcctt cacgcggcac ccgggacatg cgcaccgcgc atctgcttgc
     7561 cgc
//
LOCUS       DATIOW010000011        10472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1832602, whole genome shotgun sequence.
ACCESSION   DATIOW010000011 DATIOW010000000
VERSION     DATIOW010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10472
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832602"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..802)
                     /gene="rlmB"
                     /locus_tag="VLL03_00230"
     CDS             complement(53..802)
                     /gene="rlmB"
                     /locus_tag="VLL03_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HSS45832.1"
                     /translation="MSRLIYGFHAVTASIRQSAENVQEIYLDQARQDHRAKDLLALAE
                     SRKVRVILVDSARLDGMTGQAKHQGVAAKVSAVSHTADLVELLKNLSEPALLLVLDGV
                     QDPHNLGACLRVADACGVHVVIAPKDRAVGITPTVSKVACGAAEIVPYIAVTNLARTM
                     RELKERGIWIIGADSKAEKDLFSVKLNGPIAWVLGAEEEGLRRLTRETCDELVHIPMA
                     GGVESLNVSVASGICLFETRRQRASKNAKPG"
     gene            complement(799..2937)
                     /gene="rnr"
                     /locus_tag="VLL03_00235"
     CDS             complement(799..2937)
                     /gene="rnr"
                     /locus_tag="VLL03_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011884656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HSS45833.1"
                     /translation="MPSREFILKILSEQGVPVSAEKLMDILGIGAQESALFHRRLRAM
                     ERDGQIMCNRKGAICVVDKLDLIKGRVQGHADGFGFLIPDDGGTDLFIGPGEMHKVLH
                     GDRVMAREIGVDRRGRPEAKIVEVLARANQRVVGRLHDEHGVLLVAPEDKRISQDILI
                     AKGESSGAEAEQVVTVEIIEQPSKHAQPIGRVVEVLGNYADPGMEIEIALRKHDLPYM
                     FPPEVERLCRSFSSGVEQKDYAGRKDLRRLPLVTIDGETAKDFDDAVYCELHGGEFKL
                     FVAIADVSHYVKTGDALDQEASARGNSVYFPRRVIPMLPEVLSNGLCSLNPNVDRLAV
                     VCEMEIDADGEIGNYRFYPAVFRSHARLTYTEVWDMLQHPQSETARRYVKLLPYLQML
                     YRLYKVLAKAREKRGAIDFETIETQMVFNQQGKIERIVPVKRNDAHRLIEECMLAANV
                     CASDFLKQHGQKTLYRVHEGPTPEKLEALRGFLKEFGLQLGGGDNPHASDYAKLLIQT
                     RDRPDAQLLQTSMLRSLQQAMYSPDNVGHFGLAYESYTHFTSPIRRYPDLLVHRAIKA
                     VLNGKTYNPGDWNELGIHCSQTERRADDATRDVEAWLKCYYMQDKINECFDGTVSAVT
                     GFGIFVALDDIYIEGLVHISELGSDYFHFDARKQMLLGERTGQKYRLGDRVRVKVVRV
                     DLQSSKIDFVLEDAPEASGKRSRQAKRART"
     gene            3115..3201
                     /locus_tag="VLL03_00240"
     tRNA            3115..3201
                     /locus_tag="VLL03_00240"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3149..3151,aa:Leu,seq:cag)
     gene            complement(3291..4583)
                     /locus_tag="VLL03_00245"
     CDS             complement(3291..4583)
                     /locus_tag="VLL03_00245"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HSS45834.1"
                     /translation="MSKNVVVVGTQWGDEGKGKVVDWLTDHAQGVVRFQGGHNAGHTL
                     VIAGRKTVLHLIPSGILRAGVHCYIGNGVVVSPQALLEEIATLKRAGVEVVGRLKISE
                     ACPLILPYHAALDQAREAAKGAGKIGTTGRGIGPAYEDKVARRAIRLQDLFQRERFAA
                     KLGEVLDYHNFVLKNYFHAKPVDFQRTLEQTLALAEPIKPLVADVPRLLYQANREGKN
                     LLFEGAQGTLLDIDHGTYPFVTSSNCVAGAASAGSGIGPHLLHYVLGITKAYTTRVGS
                     GPFPTELSDEVGKLLASRGQEFGATTGRPRRCGWFDAAVLKRSIQINGVSGLCVTKLD
                     VLDGMERLQLGVGYKMDGEESDILPGGAELLAKCEPIYEEIPGWKESTVGIKRLEDLP
                     RPAQNYLRRIEQICDVPIDIISTGAEREETIVLRHPFK"
     gene            complement(4699..5856)
                     /locus_tag="VLL03_00250"
     CDS             complement(4699..5856)
                     /locus_tag="VLL03_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HSS45835.1"
                     /translation="MRNWLLPEYIEDILPPEARRIERLRRNILDLFYVHGYQLVIPPL
                     IEYVESLLTGSGRDMDLKTFKLVDQLSGRMMGLRADITPQVARIDAHLLNRKGVTRLC
                     YCGSVAHTLPSAMTRTREPLQIGAEVYGHAGVESDVEIHRLMLQALKLVGINEIHLDL
                     GHVKVFKAIAGRAGIAPELEAELFGALQSKDVPAVRNLARGLDARVSKALLLLAELNG
                     GDEVLQRAQKELPDYPEIAVALADLRTLASQLRGEVASLCFDLAELRGYHYHSGVVFS
                     AYAKGSTNAVALGGRYDEVSKAFGRPRAATGFSMDLRELAALLSADGASSAILAPYAQ
                     DAALQKRIAELRAQGEVVVVDLPGHEDSCGELNCDRQLVSRNGKWEIAPIK"
     gene            complement(5868..6053)
                     /locus_tag="VLL03_00255"
     CDS             complement(5868..6053)
                     /locus_tag="VLL03_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HSS45836.1"
                     /translation="MSKLLLIAFALMLVLEGVLPFLLPSIWREAFKRLTEMTDGQIRF
                     IGLSSMLVGLLLLYLVR"
     gene            complement(6061..6936)
                     /gene="hflC"
                     /locus_tag="VLL03_00260"
     CDS             complement(6061..6936)
                     /gene="hflC"
                     /locus_tag="VLL03_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HSS45837.1"
                     /translation="MRRASAVLVTLIVALVLASLGMFTVDQRQNAIVFRLGEIISIKK
                     EPGLYFKVPLLDNVRYFDTRILTFDSPEPERFLTSEKKNVLVDSFVKWQIADVRQYYI
                     SVGGDEQRAQLRLAQTVNSSLRDEFGNRTVHDVVSGERDQIMELMREKANEDARKIGV
                     HVLDVLIKRVDLPQEVSESVYRRMEAERKRVANELRSTGAAESEKIRADADRQREIIL
                     AEAYREAQRVKGLGDARAAAIYAEAYQQNPEFYAFYRSLEAYRQSFKSKNDVLVLDPN
                     SEFFKYLKHASKPGK"
     gene            complement(6933..8114)
                     /gene="hflK"
                     /locus_tag="VLL03_00265"
     CDS             complement(6933..8114)
                     /gene="hflK"
                     /locus_tag="VLL03_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020693835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HSS45838.1"
                     /translation="MALNDPQWGRRGNQGPPDLDEVWRKFNEKLNSLFGRSRGSGGGP
                     TPASPKPFGGGLGLLAILVLTVWLASGIYIVDASQRGVVLRFGKYVETTQPGPHWRLP
                     YPIESVEIVNLSQVRTKEVGYRNNVKSKVLTESLILTDDENIIDIQFAVQYILKSPED
                     YLFNNRTPDDTVLQVAETAIREIVGKSKMDFVLYEGREQVAAHATRLMQEILDRYKTG
                     ILISKVTMQNAQPPEQVQAAFDDAVKAGQDRERQKNEGQAYANDVIPRARGGAARLLQ
                     EGEGYRQRVIANAEGDASRFRQILVEYSKAQAVTRDRLYLDMMQQVLSSTSKVLIDQK
                     SGANLLYLPLDKLLQSGGSAATTQELPPRAATTPEPAPQPESTSRSREAFRSRDREQR
                     Q"
     gene            complement(8101..9285)
                     /gene="hflX"
                     /locus_tag="VLL03_00270"
     CDS             complement(8101..9285)
                     /gene="hflX"
                     /locus_tag="VLL03_00270"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HSS45839.1"
                     /translation="MFERPDSGSTAVLVGLDFGEAGYEESLQELKLLARSAGINARSL
                     IQGKRSKPDPTFFAGTGKVGELALALHQTFAQVVIFNHELSPAQERNLEKQLRCRVVD
                     RTSLILDIFARRAKSHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLARRVKVLKDKLTRLCRQREVQRRARRRTNMMSVSLVGYTNAGKSTLFNSLT
                     HAHAYCADQLFATLDTTTRKLFVEGHGTVALSDTVGFIRSLPHTLVEAFRATLEETAQ
                     ADLLLHVVDASHPDRERQIEEANKVLAEIGADALPQILVLNKIDLCGGKAGVERDEYG
                     KITRIRLSAQSAEGIELVRQALLETSEFPESAKVPIAPGLNREPAVRDKTELCSKHYG
                     IE"
     gene            complement(9299..9535)
                     /gene="hfq"
                     /locus_tag="VLL03_00275"
     CDS             complement(9299..9535)
                     /gene="hfq"
                     /locus_tag="VLL03_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HSS45840.1"
                     /translation="MTKGQLLQDPFLNALRKEHVPVSIYLVNGIKLQGQIESFDQYVV
                     LLKNTVTQMVYKHAISTVVPARPVSIPFEPSPES"
     gene            complement(9627..>10472)
                     /gene="der"
                     /locus_tag="VLL03_00280"
     CDS             complement(9627..>10472)
                     /gene="der"
                     /locus_tag="VLL03_00280"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HSS45841.1"
                     /translation="DAPDEETPLQQDTHPKIAIVGRPNVGKSTLVNALLGEERVIVFD
                     EPGTTRDSIYVDFERQGKRYTLIDTAGLRKRGKAVETVEKFSMLKTLQAVADANVAVL
                     VLDSQNQITEQDAHIAGIILEAGRALVVALNKWDGLGWAEREQVKRDLAWKLKFLDFA
                     QVHTISAREGQGLVALMKAVDAAYAAAMAKLPTPRLTRVLMGAVIRQQPPRSGLIRPK
                     LRYAHQGGRNPPRIVVHGNALDSVPDSYWRYLEGVFRAAFKLQGTPLKVEFKRSRNPY
                     AGKKA"
BASE COUNT         2020 a         3223 c         2875 g         2354 t
ORIGIN      
        1 taaagcggat ccgttaattc agaagttaca atgattttgc aagcatagtt tctcagccgg
       61 gctttgcgtt tttagaggct ctttgccttc tcgtttcaaa cagacaaatc ccgctggcca
      121 ctgatacatt caggctttcc acgccgcccg ccatgggaat gtggaccagt tcgtcgcagg
      181 tttcacgggt aagacgccgc agcccctctt cttcagcgcc cagcacccaa gcgatagggc
      241 cattaagttt taccgaaaac aaatcttttt ccgccttgct gtccgcaccg attatccaaa
      301 tgccgcgttc ctttaattcg cgcattgtcc tggcgagatt ggtgacggca atataaggta
      361 cgatttccgc cgcgccgcag gcgactttgg ataccgtggg agtaattcct accgcgcgat
      421 ctttgggggc gatgaccaca tgcacaccgc aggcatccgc cacgcgcagg caggcaccca
      481 gattatgcgg atcctgcacg ccgtcgagca ccagtaacaa ggcgggttca ctcaaattct
      541 tcagcaattc aaccaaatcg gcggtatggc tcactgcgct gactttggcc gccacgccct
      601 gatgcttggc ctggccggtc atgccatcaa gccgcgctga atccaccaga atcacgcgca
      661 ccttgcggct ctccgccaat gccagtaaat ctttcgcgcg atgatcctgg cgcgcttgat
      721 ccagatagat ttcctgcacg ttttccgcgc tttgccggat gctcgcggtc accgcgtgga
      781 aaccgtaaat caagcggctc atgttcttgc cctttttgcc tgccggctcc ttttgcccga
      841 tgcctcggga gcatcttcaa gaacaaaatc gatttttgag ctttgcaaat ccactctcac
      901 cactttgact ctcaccctat cgcctagccg gtatttctgg ccggtacgct cgcccagcag
      961 catctgcttg cgggcatcga agtggaaata atcgctgccg agctcggaga tatgcaccag
     1021 cccttctata taaatgtcat cgagcgccac gaaaatgccg aagcccgtca ccgcgctcac
     1081 cgtgccgtcg aagcattcgt tgattttatc ctgcatgtag tagcacttga gccaggcttc
     1141 gacatcgcgt gtcgcgtcat cggcgcgccg ctcggtttgc gagcaatgaa tgcccaattc
     1201 gttccaatcg cccggattgt aggtcttgcc gttcagcacc gctttgatgg cgcggtgcac
     1261 cagcaaatcg ggatagcggc ggatgggcga ggtaaagtgc gtgtaggatt cgtacgccaa
     1321 accgaaatgc ccgacgttgt cggggctgta catcgcttgc tgcagcgagc gcagcatcga
     1381 ggtttgcagc aattgcgcat ctggacggtc tctagtttga ataagcagct tcgcgtaatc
     1441 gctggcatgc ggattgtcgc ccccgcctaa ttgcaaaccg aattccttga gaaatccccg
     1501 cagcgcttcc agtttttccg gcgtgggacc ttcatgcacc cgataaagcg tcttttggcc
     1561 gtgttgcttg agaaaatccg aagcgcagac atttgccgcc agcatacatt cctcaatcag
     1621 ccggtgggca tcgttgcgct tcactggcac gatgcgctcg attttcccct gctggttgaa
     1681 aaccatctgc gtttcgatgg tctcgaaatc aatcgcgccg cgtttttcac gcgctttcgc
     1741 cagcaccttg taaagcctgt agagcatttg caggtacgga aggagcttga cgtagcggcg
     1801 cgcggtttcg ctttgcggat gctgcagcat gtcccatacc tcggtatagg tgagccgggc
     1861 gtgcgagcgg aacaccgccg gataaaagcg gtagttgcca atttcaccat cagcatcaat
     1921 ttccatttca cacaccaccg ccagccggtc cacgttcgga ttgagcgaac acagcccgtt
     1981 actcaacact tctggcagca tcggaatcac ccgccgcgga aaatacaccg agttgccgcg
     2041 tgctgatgcc tcctgatcca gcgcatcgcc ggtctttacg taatggctga catcggcgat
     2101 ggcgacgaac aatttaaatt ccccgccatg gagttcgcaa tacaccgcgt cgtcgaaatc
     2161 cttggcggtt tcgccgtcta tcgtcaccag cggcaagcgc cgcaggtctt tgcgacccgc
     2221 ataatccttt tgctccactc cgctggaaaa gcttcggcac agcctttcca cttcaggcgg
     2281 gaacatatac ggtaaatcgt gcttgcgcag cgcaatttcg atttccatgc ccggatcggc
     2341 gtaattaccc agcacttcca ccacgcggcc gatgggttgc gcgtgcttgc tcggctgctc
     2401 gatgatttcc accgtcacca cctgctctgc ctcggcgccg ctgctttcgc ctttggcaat
     2461 cagaatgtcc tggctgatgc gcttgtcttc aggcgccacc aacaggacgc cgtgctcgtc
     2521 atgcagccgc cccaccacgc gctgattggc acgcgcgagc acttcgacga tcttggcctc
     2581 cggccggccg cgccgatcca cgccgatttc gcgcgccatc acgcggtcgc cgtgcagtac
     2641 cttgtgcatt tcaccggggc cgatgaatag atccgtcccg ccgtcatccg gtatcaaaaa
     2701 accaaagccg tctgcgtgtc cctgcaccct gcccttgatc agatcgagct tatccaccac
     2761 gcaaatcgcg cccttgcggt tgcacatgat ttgcccgtcg cgctccattg cgcgcagacg
     2821 ccggtgaaac agcgcgcttt cctgcgcacc gatgccgaga atgtccatca gtttttccgc
     2881 ggaaacggga acgccctgct cggaaaggat tttcagtatg aattcgcggc tgggcatagc
     2941 gtagggatat ttggcacgtt cgcgtttgag atgcgggtca agctcccgca atttggactt
     3001 ggcttttgcc gccggacgcg attggcgccg cgctccagcc gaatgtagat gagaattact
     3061 gcgtttcgtt gacaagaaag tgttttccta ttagatttgc acctctcgta aagagccgag
     3121 atggcggaat tggtagacgc accaggttca ggtcctggcg gtggcaacac tgtggaggtt
     3181 cgagtcctct tctcggcacc atacggcagg attcaggatc aaggatgccg gattcaacaa
     3241 aacttttcct gccttttcca aaatcccgat tcctgagtcc cgaatcccgg tcatttgaac
     3301 gggtggcgca acacgatggt ttcttcgcgt tctgccccgg tcgaaattat atcaatcggc
     3361 acatcgcaaa tttgctcgat gcgcctcaag taattctgcg ccgggcgcgg taaatcttcc
     3421 aatcgcttta tccccaccgt gctttctttc cagcccggaa tttcttcgta gatgggctcg
     3481 catttggcca acagctccgc cccccccggc aggatatcgc tttcctctcc atccattttg
     3541 tagcccacac ccagctgcag gcgttccatg ccgtcgagca cgtcgagctt ggtgacgcac
     3601 aggcccgaga cgccgttgat ttgaatcgag cgcttcaaca ccgccgcatc gaaccagccg
     3661 caacgccgcg gacgcccggt ggtggcgcca aattcctggc cgcgcgatgc cagaagcttg
     3721 cctacttcat cgctcaattc cgtcggaaac ggcccggaac ccacgcgcgt ggtataagcc
     3781 ttagtgatgc ccagcacgta atgcagcaag tgcgggccga tgccgcttcc cgccgacgcc
     3841 gcgccggcga cgcaattgct ggacgtcaca aaggggtagg tgccgtggtc aatatcgagg
     3901 agcgtgcctt gcgcgccctc gaacaaaaga tttttgcctt cacgattggc ctgatacaaa
     3961 agccgcggca catcggcgac cagcggctta atcggctcgg ccagcgccag cgtttgttcc
     4021 agcgtgcgct gaaaatccac cggtttggcg tgaaaataat ttttcagtac gaagttgtga
     4081 taatccagca cttcgcccag tttcgctgcg aagcgctcgc gctggaacag atcctgcaaa
     4141 cgaatcgcgc gccgcgccac tttgtcttca taagccgggc cgatgccgcg cccggtcgtg
     4201 ccgatcttgc cggcgccttt cgccgcttcg cgcgcctgat ccaaggccgc gtggtaaggc
     4261 agaatcagcg ggcaggcttc gctgatcttg agcctcccca ccacctccac tccggctcgc
     4321 ttgagtgtag cgatttcctc cagcagcgcc tgcggcgaga ccaccacgcc gttgccgatg
     4381 tagcaatgca cgcccgcgcg cagaatgccg gaaggaatca gatgtaaaac agttttcctg
     4441 ccggcgatga ccaacgtgtg gccggcattg tgcccgccct ggaaacgcac cacgccttgg
     4501 gcgtgatcgg tcagccagtc gacgaccttg cctttgcctt catcccccca ttgggtgccg
     4561 acgacgacga cgtttttgga catgcccggt caatttcctt tagttaggct ttttcaccga
     4621 cccagagaac ggaagacaaa cattcttgaa taataatgtt gtaatgaatt tctccgtgac
     4681 ttgtgtgtct ccgtggtttc acttaatcgg cgcgatttcc cacttgccgt tgcgcgacac
     4741 cagctggcgg tcacaattca attcgccgca actgtcttca tgcccgggca aatccacgac
     4801 gacgacttcc ccttgcgcgc gcaattcggc gatgcgcttt tgcagagccg cgtcctgcgc
     4861 ataaggcgcg aggatggccg aagacgctcc gtcggcagac agcagcgcgg ccagctcgcg
     4921 caagtccatg ctgaagccgg tcgcggcgcg cggacggccg aatgccttgc ttacttcgtc
     4981 atagcgtccg cccagggcaa cggcattggt cgaacctttg gcataggcgc tgaacaccac
     5041 gccgctgtgg tagtggtagc cgcgcagctc cgccagatca aagcacaggc tcgccacctc
     5101 gccgcgaagt tgagaagcca gcgtgcgcaa atcggcaagc gcgacggcga tttccggata
     5161 atccgggagt tccttttgcg cacgctgcaa gacctcatcc cctccgttga gttccgcaag
     5221 caaaagcagc gccttgctta cccgcgcatc aagaccacga gcaagattcc gcaccgccgg
     5281 cacgtccttg gattgcagcg cgccaaacag ttcggcttca agttcaggcg cgatgccggc
     5341 gcggccggca atggctttga ataccttgac atggccgagg tcgagatgaa tctcattgat
     5401 gcccacgagc ttcagcgcct gcagcatcag ccgatggatt tccacgtcgc tctccacgcc
     5461 ggcgtgaccg taaacttcag cgccgatttg cagcggctcg cgcgtgcgcg tcatggccga
     5521 tggcaaggtg tgcgcgacgc tgccgcaata gcacaggcgg gtgacaccct tgcggttcag
     5581 gaggtgcgcg tcgatgcgcg ccacctgcgg cgtaatgtcg gcgcgcaagc ccatcatgcg
     5641 gccgctcaat tggtccacca gcttgaaggt tttcaaatcc atatcgcgcc cgctgccggt
     5701 gagcagcgat tcgacatatt caatgagcgg cgggatcacc aattgatagc cgtgcacata
     5761 aaacaggtcg aggatgttcc ggcgcagccg ctcgatgcgg cgcgcctccg gcggcaggat
     5821 gtcttcgatg tattcgggca gcagccagtt gcgcataaga acctatctca tcgcaccaga
     5881 taaagcagca gcaaaccgac aagcatcgag ctcaagccga taaagcggat ttggccgtcg
     5941 gtcatttcgg taagtctctt gaaggcttcg cgccagatgg acggcagcag aaacggcaac
     6001 acgccttcca gcaccaacat cagggcgaac gcaatcagca acagtttgga catggcgcgc
     6061 ctacttgccc ggtttgctgg cgtgtttcag gtatttgaag aactccgaat tggggtccag
     6121 caccagcacg tcgtttttgc tcttgaaact ttgccggtag gcttcgagac tgcggtaaaa
     6181 agcgtaaaac tccggattct gctgataagc ctcggcgtaa atcgccgcgg cccgggcatc
     6241 gcccaggccc ttgacgcgct gcgcctcgcg gtaggcttcg gcgaggatga tttcacgctg
     6301 gcggtcggca tcggcgcgga ttttttccga ttctgccgcg ccggtggaac gcagttcgtt
     6361 agcgacgcgt tttctttccg cttccatgcg acggtaaacc gattcgctca cttcctgcgg
     6421 caggtccacg cgcttgatga gcacatccag cacatgcacg ccaatcttgc gcgcgtcctc
     6481 gttggccttt tcccgcatca gctccatgat ctgatctcgc tcgccggata ccacatcatg
     6541 caccgtgcga ttgccgaatt cgtcgcgcag gctggaattc accgtttgcg caagccgcaa
     6601 ctgcgcgcgc tgctcgtcgc cgccgacgct gatgtaatac tgcctgacgt ccgcgatttg
     6661 ccatttgacg aacgaatcca ccagaacgtt tttcttttcc gaggtgagaa agcgctccgg
     6721 ctcgggggaa tcaaaagtga gaattcgcgt atcgaaatag cgtacgttgt cgagcagcgg
     6781 caccttaaaa tacagtccgg gttctttttt aatcgagatg atttcgccca agcgaaacac
     6841 gatggcgttc tgccgctggt ccacggtgaa catgccgagg ctcgccagca ccagcgccac
     6901 gatcagggta accagtaccg cgcttgccct tctcattggc gttgctcccg gtcgcggctg
     6961 cggaacgctt cgcgcgaacg cgaggtgctc tccggctgcg gtgcaggctc aggtgtcgtg
     7021 gcggctctcg gtggaagttc ctgcgtggtc gccgccgagc caccgctctg caacagcttg
     7081 tccaaaggca ggtacagcag attggctccg gacttctggt ctatcaacac cttgctggtg
     7141 ctggaaagca cctgctgcat catgtccagg tacaggcggt cgcgcgtcac tgcctgtgcc
     7201 ttgctgtatt ccaccaggat ctgccggaag cggctggcat cgccttcagc attggcaatc
     7261 acgcgctgcc ggtagccttc cccttcttgc agcaggcgcg cggcaccgcc tcgagccctg
     7321 ggaatcacat cgttggcata ggcctggcct tcgttttttt gccgctcgcg gtcctggccc
     7381 gctttcaccg cgtcgtcaaa cgccgcttgc acctgctccg gcggctgcgc gttttgcatc
     7441 gtcaccttgc tgatcaggat gccggtcttg tagcggtcga gaatctcctg catcaggcgc
     7501 gtggcgtgcg ccgccacctg ctcgcgcccc tcgtagagca cgaaatccat tttgcttttg
     7561 ccgacgattt cgcggatcgc ggtttccgcc acttgcagca ccgtgtcgtc cggcgtgcgg
     7621 ttattgaaca gataatcctc ggggcttttc aggatgtact gcaccgcaaa ttggatatcg
     7681 atgatgttct catcgtcggt gagtatcagc gattccgtca gcaccttgct tttcacgttg
     7741 ttgcggtagc ccacttcttt ggtgcgcacc tgcgagagat tgacgatttc cacgctttcg
     7801 atgggataag gcaggcgcca gtgtggccca ggctgtgtgg tttccacata ttttccaaaa
     7861 cgcagcacca cgccgcgctg cgaggcatca acgatataaa taccgcttgc cagccagacg
     7921 gtcaaaacca gaatggcgag caagcccagc ccgccgccga agggcttggg cgaagcgggc
     7981 gtgggcccgc cgccggaccc acggctgcgg ccgaacagcg aattcaattt ttcgttgaat
     8041 ttgcgccaga cttcgtcgag atcgggaggc ccctggttgc cgcgcctgcc ccactgcgga
     8101 tcattcaatg ccatagtgtt tgctgcacag ttcggttttg tcgcgcactg caggctcccg
     8161 gttcaggccg ggtgctatcg gtacttttgc tgattcggga aattcgctgg tttcgaggag
     8221 cgcctgccgc actagttcga tgccttctgc gctttgtgcg ctcaaacgaa tacgggtaat
     8281 tttaccatac tcgtcccgct ccacgcccgc cttcccgccg cacaaatcga tcttgttgag
     8341 caccagtatc tgcggcaagg catccgcgcc gatttccgcc agcactttgt tcgcttcctc
     8401 aatctgccgc tcgcggtcgg gatggctcgc gtccaccaca tgcagcagca aatccgcctg
     8461 cgcggtttct tccaatgtgg cgcggaaagc ttccaccagc gtgtgcggaa gactacggat
     8521 aaagccaacc gtatctgaca acgccaccgt gccatgaccc tcaacaaaca atttgcgcgt
     8581 agtagtatcc agcgtggcaa aaagctggtc ggcgcaataa gcgtgggcgt gagtaaggct
     8641 gttgaagagc gtggacttgc cggcgttggt gtagcccacg agagataccg acatcatgtt
     8701 ggtgcgacga cgcgcccttc tctgcacttc gcgctggcgg cacagtcttg tcaatttatc
     8761 cttgagcacc ttgacgcgcc tggcgagcaa acgccggtcg gtttccaact gggtctcacc
     8821 gggaccccgc aatccgatgc cgcccttttg cctttccaag tgcgtccagc cgcgtaccag
     8881 gcgggtggcg agatgcctaa gctgcgcgag ctccacttga agtttgcctt catggctttt
     8941 ggcgcgcctg gcgaagatgt cgagaatgag gctggtgcgg tccaccaccc ggcagcgtaa
     9001 ctgcttttcc agattgcgct cctgggccgg ggacaattcg tggttgaaga tgacgacttg
     9061 cgcgaaagtt tggtgcaaag ccagcgcaag ttccccgact ttgccggtgc ctgcaaaaaa
     9121 cgtggggtca ggttttgagc gttttccctg gatcaagctg cgggcgttga tgccggcgct
     9181 tctagccagg agcttgagtt cctgcaggct ttcctcgtag ccggcttcgc caaaatcgag
     9241 gccaactaat actgccgtac tcccgctgtc gggacgctcg aacattaaaa ctttaacgtc
     9301 aactttccgg ggatggctcg aacggtatgc tcaccggcct agcgggaacc acggtggaaa
     9361 tggcgtgctt gtataccatt tgcgtgactg tgttcttcag caacaccaca tactggtcaa
     9421 acgattcgat ttgcccttgc agtttgatgc cgttcaccag ataaatcgaa accggcacat
     9481 gttccttacg tagagcgttc aaaaacgggt cttgtaacaa ttgccctttg gtcatgttga
     9541 ctccaatttt agttattggg tgtttaactt tacttgattt tcgggcttaa agccagccgt
     9601 cgagcccggc ggcaaatact ttgttttcaa gcctttttcc cggcataggg gttgcggctg
     9661 cgcttgaatt ccaccttgag cggcgtgccc tgcagtttga atgccgctct aaatacgccc
     9721 tccagatagc gccaatagct gtccggcacc gaatccaggg cattgccgtg aaccacgatg
     9781 cgcggcgggt tgcggccgcc ttgatgggcg tagcgcaatt tcgggcggat gagccccgaa
     9841 cgcggcggtt gctgcctgat gaccgcgccc atcaagacgc gggtcagcct gggggtgggg
     9901 agtttcgcca tcgccgccgc atacgccgca tccaccgctt tcatcaaggc gactaggccc
     9961 tgtccttccc gcgccgaaat agtgtggacc tgggcgaagt cgaggaactt gagcttccac
    10021 gccagatcgc gcttaacctg ttcgcgctcg gcccagccaa ggccgtccca cttgttcaac
    10081 gccaccacca aggcccgccc cgcttccaga atgatgccgg caatgtgcgc gtcctgttcg
    10141 gtgatctggt tttgcgaatc gagaaccagc accgccacat tggcatccgc caccgcctgc
    10201 agggttttga gcatggaaaa tttctcgacc gtctccaccg ccttgccgcg cttgcgcaag
    10261 ccggcggtgt cgatcagcgt gtaacgcttg ccttgccgct cgaaatccac gtaaatgctg
    10321 tcgcgggtgg tgccgggttc atcgaacacg ataacccgct cctcgcccag cagggcgtta
    10381 accaaagtgg atttgccgac gttgggacgg ccgacgatgg cgattttcgg gtgagtgtct
    10441 tgctgcagcg gcgtttcctc gtccggcgca tc
//
LOCUS       DATIOW010000012         1500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_3981, whole genome shotgun sequence.
ACCESSION   DATIOW010000012 DATIOW010000000
VERSION     DATIOW010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3981"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VLL03_00285"
     CDS             <1..470
                     /locus_tag="VLL03_00285"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HSS45842.1"
                     /translation="EQVDVVRRFGSKVYYGDASRLELLRAAKADRAKIFVLAIDNVAS
                     SLKTAATVKKHFPNLAIYARARNRFHAYQLMDIGVDGLIRETFLSSLDLARRVLWGLD
                     IGEEQARATVEMFKAHDEKTLLAQHAIHHDETKLIQTAKEAAQELQALFETDT"
     gene            complement(516..>1500)
                     /locus_tag="VLL03_00290"
     CDS             complement(516..>1500)
                     /locus_tag="VLL03_00290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HSS45843.1"
                     /translation="IEAQLAEVEAAEATRRDVIVSLIAEVARNYFELRGTQHQLDVAL
                     KNATNQHETLNLTLILLEGGQGTELDTSRAREQWNSTLATIPLLQAAVQRAIYRLSVL
                     TGQQPQALLAEFLEPAPLADYSKPITIGKPDELLRRRSDIRFAERSLAAATALIGVAT
                     ADLFPRVTFIGNAAFEANTFSGLGNSGSGTYSFGPSIRWAAVFDYGRVRARILAADAR
                     AEANLARYERTVLTALEETENALVDFGRQRARRDYLRAATEASEKAAVLARQRYQEGA
                     TDFLTVLDSERRLLEAQENLAQSETNTATALVAVYKALGGGWEIEQPLAAK"
BASE COUNT          337 a          445 c          407 g          311 t
ORIGIN      
        1 aagagcaagt ggatgtagtg cggcgattcg gcagcaaggt ctattacggt gatgcctcac
       61 gcttggagct gctgcgcgcc gccaaagcag accgggcgaa aattttcgtc ctggcaatcg
      121 acaatgtcgc ctcgtccctc aaaaccgccg cgacagtaaa aaagcacttt cccaacctgg
      181 caatttatgc ccgcgcccgc aaccgtttcc atgcttacca gctgatggat ataggcgtag
      241 atggcctgat acgggaaacg tttctctctt ctttagattt agctaggcgg gtattgtggg
      301 gcctggacat tggggaagag caggctcgcg ccaccgttga aatgtttaag gcccatgatg
      361 aaaaaacgct gcttgcgcaa catgcgattc atcacgacga gaccaaactg atacaaaccg
      421 caaaagaagc cgctcaagag ctgcaggcac tgttcgaaac cgatacctaa tcctgaaaaa
      481 catcaaagcg tcgcgcaagc ttaattaaat ttgattcact tcgcggcaag cggctgctcg
      541 atttcccaac ccccgcccag cgctttatac acggccacca gcgcagtcgc agtgttggtt
      601 tcgctctgcg ccaagttttc ctgtgcctca agtaagcggc gctcagaatc aagcaccgta
      661 agaaaatcgg tggcaccctc ctggtagcgc tgccgcgcca gaacagccgc cttttcgctg
      721 gcttccgttg cggcacgcaa ataatcacga cgcgcgcgtt gacggccgaa gtcgaccagc
      781 gcgttttcag tctcttcaag agctgtcagg acggttcttt cgtaccgtgc gaggttcgct
      841 tccgcgcgcg cgtcagccgc aagtatgcgc gccctgacac gtccatagtc aaagaccgcc
      901 gcccaacgga tcgagggtcc gaatgagtaa gttccgctgc ccgaattccc tagtccagaa
      961 aaagtgttgg cctcgaacgc cgcattgccg ataaaagtca cccgcgggaa caggtcggca
     1021 gtcgccaccc cgataagagc cgtggcggcg gcgaggctgc gctccgcgaa gcggatgtcg
     1081 gagcgacgcc gcagcaattc atcgggtttg cctatcgtga ttggctttga ataatccgcc
     1141 agaggtgccg gttccaggaa ctcagcaagc aatgcctggg gttgctgccc cgtcaacacg
     1201 ctgagacggt aaatcgcccg ttgcaccgca gcctgcaaca gcggtatggt ggccagagtt
     1261 gaattccatt gctcgcgcgc ccgcgaagta tccaattccg tgccttgccc tccctccaga
     1321 agaatcagcg tgagattaag ggtttcatgc tggttagtag cgtttttcag cgccacatca
     1381 agttgatgct gagtgccgcg aagctcgaag taattccgcg cgacctccgc aatcaggctc
     1441 acgatcacgt cgcggcgcgt ggcttcggca gcttcaactt cggccaactg cgcttcgatc
//
LOCUS       DATIOW010000013         6584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1426563, whole genome shotgun sequence.
ACCESSION   DATIOW010000013 DATIOW010000000
VERSION     DATIOW010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426563"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VLL03_00295"
     CDS             complement(<1..455)
                     /locus_tag="VLL03_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HSS45844.1"
                     /translation="MPLTDTAIRNAKPGEKPVKMFDERGLFLLVIPSGGRWWRFKYRF
                     DGKEKQLSFGVYPDVGLKEARERRDEARKQVAAGIDPAENRKAMKTARADRAANSFEV
                     VAREWFAKYSPNWATSHADKIIQRLEKDVFPWIGSKPIAKITAPELLTVL"
     gene            complement(619..694)
                     /locus_tag="VLL03_00300"
     tRNA            complement(619..694)
                     /locus_tag="VLL03_00300"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(659..661),aa:Ala,seq:cgc)
     gene            complement(811..1593)
                     /gene="lgt"
                     /locus_tag="VLL03_00305"
     CDS             complement(811..1593)
                     /gene="lgt"
                     /locus_tag="VLL03_00305"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012212886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HSS45845.1"
                     /translation="MLIHPQFDPVAIHLGPLSIRWYGLMYLLGFIAGLMLGRHRIKAQ
                     PQSGWTYQQLDEALFYIVLGVILGGRLGYVLFYKFADYLHEPIKIFYVWEGGMSFHGG
                     FLGVIFALWLFSRKHRKHWFAMTDFIAPLVPVGLGAGRIGNFINGELWGRPAAVPWAM
                     IFPQADNIPRHPSQLYEFTLEGVALFIILWFYSSKPRPMGAVSGVFLIGYGLFRFLVE
                     FTREPDSFLGLLALNLSMGQWLSLPMIAGGVWMLWWSYRNRT"
     gene            1667..3340
                     /gene="ilvD"
                     /locus_tag="VLL03_00310"
     CDS             1667..3340
                     /gene="ilvD"
                     /locus_tag="VLL03_00310"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HSS45846.1"
                     /translation="MPHNSRSKTITQGVQRSPNRAMLRAVGFADGDFDKPIVGIANGH
                     STMNPCNAGLGPLAARAEEALKQAGAMPQSFGFPTVTDGISMGTEGMKYSLVSREVIA
                     DSIETAVQSQFMDGVLVVGGCDKNMPGSMMALLRMNVPGIFCYGGTIKPGSWHGQVLT
                     IVSPFEAVGAFSAGKMTQEDFEGIEKNACPGFGACGGMYTANTMSSSFEALGMSLLGS
                     SQMANPDPEKADSVAESARVLVNAVQKQILPRQIVTRKSIENAISLVMAAGGSTNAVL
                     HYLAIAHTAGVEWVLDDFERVRIKVPVLCDLKPSGKYVATDFHKAGGVPQVLKMLLKA
                     GLLHGDCMTITGRTMAEELAKVPDEPRSDQEVIRPFNRPIYAQGHLAILKGNLAAEGC
                     VAKITGLKNPSITGRARVFNSEDEAMAAILGDKIKAGDVLVIRYEGPKGGPGMREMLA
                     PTSAIIGKGLGESVGFITDGRFSGGTWGMVVGHIAPEAFVGGTIALVKEGDSITIDAR
                     KLLIQLNVPEAELARRKAAWQPPKPRYTRGVMAKYAKLVSSASTGAVTD"
     gene            3377..5482
                     /locus_tag="VLL03_00315"
     CDS             3377..5482
                     /locus_tag="VLL03_00315"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HSS45847.1"
                     /translation="MSNHLASETSPYLQQHADNPVDWHPWGEEALRLSREQNKPILLS
                     IGYSACHWCHVMAHESFEDEEVAAIMNQHFINIKVDREERPDLDQIYQAAHYMLTQRG
                     GGWPLTLFLTPEQKPFFGGTYFPKQPRYNLPGFGDLLKRIAGFYHEHRDEIEKQNESL
                     LAAFERLEPDKPSRPQEPDAEPLQRLFTQLQNIFDPVHGGFGDAPKFPHPAEIEFCLR
                     RFAASGDSVALHFACFTLEKMAQGGIYDQLGGGFSRYSVDQYWMIPHFEKMLYDNGPL
                     LRLYTDAWLAAKNSLFKTVAEETARWVLREMQSPEGGYYSTLDADSEHEEGKFYIWRR
                     EEVSSLLTADEYAVLAPYYGLDRAPNFEGGFWHLNICKSLDEVAKTLSISNEAARHRL
                     DGARQKLFAVREQRVHPGRDEKILASWNGLMIKGMARAARVFGHENWLASAQRACDFV
                     RNALWRDGRLLATYKGAPGADATTQHSGISAKGSTGRAHLNAYLDDYAFMLDALLELL
                     QTQFRKADFDFAKDLAEVMLEQFEDRELGGFFFTSRDHEKLIHRPKAGHDNATPSGNG
                     IAIFALQRLGHLLGEPRYLEAAERALKLFYPEVSSRPSGYTTIAIALEEFLAPPQTVI
                     LRGEAEAVREWQQELGREYLPHALILALPKGLAGLPQTLDREAKGKVNAWVCQGVKCL
                     PAINKLQELKQVCKTGQAG"
     gene            5514..5840
                     /locus_tag="VLL03_00320"
     CDS             5514..5840
                     /locus_tag="VLL03_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSS45848.1"
                     /translation="MRTLLIGLLSGLALATASANALADAKAGEALAKKNNCFACHAVD
                     SKLVGPAYKDVATKYKGDKSAEARLIEKVKKGGSGVWGQIPMPPNSPQVKDEDIKTIV
                     QWVLSL"
     gene            5982..>6584
                     /locus_tag="VLL03_00325"
     CDS             5982..>6584
                     /locus_tag="VLL03_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HSS45849.1"
                     /translation="MKFSHGFALAILAVTLGLLGCGKEQTSSSELVVKIGSVAPLTGP
                     QAHLGKDNDNGARLAIEEVNARGPVIGGKKVRFELISEDDQADPKTATIVAQKLVDQK
                     INAVIGHLNSGATIPASKIYHDADVVQISPSATAIPYTQQGFKNAFRVMTNDEQQGKV
                     LGEFAVSQMAAKKIAIIDDRTAYGQGLADQFEKAAKAGGAE"
BASE COUNT         1498 a         1929 c         1883 g         1274 t
ORIGIN      
        1 agcactgtca gcagttccgg tgccgttatc ttggcaatcg gtttactgcc gatccagggg
       61 aaaacatcct tttccagccg ctggatgatt ttgtcggcat gcgatgttgc ccagttcggg
      121 gaatacttgg cgaaccattc ccgcgccacc acctcgaaac tgtttgccgc ccggtcagcc
      181 cgcgccgttt tcatcgcctt gcggttttct gcggggtcga taccagcggc gacttgcttg
      241 cgggcttcgt cgcgccgttc gcgggcttcc ttcaatccca catcggggta gacgccaaac
      301 gacaattgct tttccttgcc gtcgaatcgg tacttgaaac gccaccatct gcccccggaa
      361 ggaattacca acaggaatag cccgcgctcg tcaaacatct tgacgggttt ttctccgggc
      421 ttggcattgc ggatcgcggt gtcagtcagg ggcatggggg caactcattg cagtggcaac
      481 tcacttgccc ctaatgttgc ccctacttgc ccccggatgt caatggacag tgttgcccct
      541 tgctggacgg aaaacaaaat aaaataccag cagttacgcg ggcttctgga tgttgctgga
      601 attgctggga tggttaaatg gtggagtcga ggaggatcga actcccgacc ttcgcattgc
      661 gaacgcgacg ctctcccagc tgagctacga ccccacttta cgagggttgc tatcatatac
      721 ttaaatgaga aaagcgtaaa ttcccggggc agaacaacgc ctgattgcgc tgccttcccg
      781 aacccttgac tcccatgtca cgcgcggtct tcaggtgcgg ttgcgataac tccaccacaa
      841 catccaaact ccgcccgcta tcatcggcag cgagagccat tgccccatgc tcaaattaag
      901 agcaagcaag cccaaaaacg aatccggctc gcgagtaaac tccaccagaa agcggaacag
      961 tccgtagcca atcaaaaaca cgcccgacac tgcacccatc ggcctgggct tggacgagta
     1021 aaaccagagg atgatgaaca gcgccacgcc ttccagcgta aattcgtaca gctgcgaggg
     1081 atggcgcgga atgttgtccg cttgcggaaa aatcatggcc caaggcacag cggcgggacg
     1141 cccccacaat tccccgttga taaaattgcc gatgcgcccg gcgccgaggc cgacgggcac
     1201 cagcggcgca atgaaatcgg tcatggcaaa ccaatgtttt ctgtgtttgc gcgaaaacag
     1261 ccatagggcg aaaatcacgc cgagaaatcc gccgtgaaaa gacatgccgc cttcccacac
     1321 gtaaaaaatc ttgatgggtt cgtgcagata atcggcaaac ttgtaaaaca gcacgtaacc
     1381 caggcgcccg ccgagaatca cgccgagcac gatgtaaaac agcgcttcat cgagttgttg
     1441 ataagtccag ccggattgcg gctgggcctt gatgcggtga cgccccagca tcagccccgc
     1501 gatgaagccg agtagataca tcagcccata ccagcgaatg gaaagcggcc cgaggtgaat
     1561 ggcaacggga tcgaactgcg gatggatgag cattggggag atcaagagtt tcagctagaa
     1621 ttgatattat acgtaaattt ctttcctggc ttactggagc ttatccatgc ctcacaattc
     1681 tcgcagcaaa actattactc aaggcgtgca gcgctcgccc aatcgcgcca tgctgcgcgc
     1741 ggtcggcttc gccgacggcg atttcgacaa gcccatcgtc ggcatcgcca acgggcattc
     1801 gaccatgaat ccctgcaacg ccggcttggg tcccctggcc gcgcgcgcgg aagaagcgct
     1861 gaagcaggcg ggcgccatgc cgcaatcttt cggctttccc accgtgaccg acggcatctc
     1921 gatgggcacg gaaggcatga agtattcgct ggtgtcgcgc gaagtgatcg ccgacagcat
     1981 cgagaccgcg gtgcagtcgc aattcatgga cggcgtgctg gtggtcggcg gctgcgacaa
     2041 gaacatgccg ggcagcatga tggcgctgtt gcgcatgaac gtgccgggca tcttctgcta
     2101 cggcggcacc atcaagcccg gctcatggca cggccaggtg ctgaccatcg tcagcccctt
     2161 cgaagcggtg ggcgcgttca gcgccggcaa gatgacgcag gaagatttcg agggcatcga
     2221 gaagaacgcc tgccccggct tcggcgcctg cggcggcatg tacaccgcca ataccatgtc
     2281 atcaagcttc gaggcgctgg gcatgagcct gctgggctct tcgcagatgg cgaatcccga
     2341 tccggagaaa gcggattccg tcgccgaatc cgcgcgcgtg ctggtcaacg ccgtccagaa
     2401 gcagattctg ccgcgccaaa ttgtgacgcg aaaatccatc gagaacgcaa tcagtttggt
     2461 gatggccgcc ggcggttcca ccaatgccgt gttgcactat ctcgccatcg cgcataccgc
     2521 gggcgtggaa tgggtgctgg acgatttcga acgcgtgcgc attaaagtac ctgtgctgtg
     2581 cgatctcaaa ccgtccggca aatacgtcgc cacggatttc cataaggccg gcggcgtgcc
     2641 gcaggtgctg aagatgctgc tgaaagcagg cctcttgcac ggcgactgca tgaccatcac
     2701 cggccgcacc atggcggaag agttggccaa ggtgcctgac gaacctagat cggatcagga
     2761 agtgattcgc ccttttaacc ggccgattta cgcacagggc cacctcgcca tactcaaagg
     2821 caatctggcg gccgaaggtt gtgtcgccaa gatcaccggc ctcaaaaatc cctccatcac
     2881 cggccgcgcg cgcgtgttca attcggaaga cgaagcgatg gccgccattc ttggcgacaa
     2941 gatcaaggcc ggagacgtgt tggtgatccg ttacgaaggt ccaaagggcg ggccggggat
     3001 gcgcgagatg ctggcgccca cctcggccat catcggcaag ggactgggcg agagcgtggg
     3061 cttcatcacc gacggccgct tctccggcgg cacttggggc atggtggtgg gccacatcgc
     3121 gcccgaagct tttgtcggcg gcacgattgc attggtcaag gagggcgaca gcatcaccat
     3181 cgatgcacgc aaattgctca ttcagctcaa tgttcccgag gcggaacttg cgcgccgcaa
     3241 agccgcctgg cagccgccca agccgcgcta cacccgcggc gtgatggcga agtatgcaaa
     3301 gctcgtttcc agcgccagca caggcgcggt gacggattaa tctgcgcttg gtgctttgac
     3361 ctagccgcgc caaaatatgt ccaaccatct cgcaagcgaa accagcccct atttgcagca
     3421 gcacgccgac aatccggtgg actggcatcc gtggggtgag gaagcgctga gactttcgcg
     3481 cgagcagaac aagccgattc tgctttccat cggttattcc gcctgccact ggtgccatgt
     3541 gatggcgcac gagtcgtttg aagatgaaga agtggctgcg atcatgaacc agcacttcat
     3601 caacatcaag gtggaccgcg aggagcgccc ggatctggat cagatttacc aggccgcgca
     3661 ttacatgctc acccaacgcg gcggcggctg gccgctcacc ttatttctca ctcccgagca
     3721 gaaaccgttt ttcggcggca cttattttcc gaaacaaccg cgctacaacc tgcccggctt
     3781 tggcgatctg ctgaagcgca ttgcgggttt ctaccatgag caccgggatg aaattgaaaa
     3841 gcagaacgag tcgctgcttg ccgcttttga gcgcctcgaa cctgacaagc cttctcggcc
     3901 gcaggagccg gatgccgaac ccttgcagcg cctgtttaca caactccaaa acatttttga
     3961 cccggtgcat ggcggctttg gcgatgcgcc gaagtttccg catccggccg aaattgaatt
     4021 ctgtctgcgc cgattcgccg cgagcggcga cagcgtcgca ctgcacttcg cctgcttcac
     4081 gctggagaaa atggcccagg gcgggattta cgaccagttg ggcggcggct tttcccgtta
     4141 cagcgtggat caatactgga tgattccaca cttcgagaaa atgctctatg acaacggccc
     4201 gctgctgagg ctgtataccg acgcctggct cgccgccaaa aattccttgt tcaagacggt
     4261 agcggaggaa accgcacggt gggtgctgcg cgaaatgcaa tcgcccgaag gcggttatta
     4321 ctcgacgctc gacgccgact ccgaacatga agaaggcaag ttctacatct ggaggcgcga
     4381 ggaggtttct tccctgctca ctgccgatga atacgcggtg ctagcgccgt attacgggct
     4441 ggatcgcgcg cccaattttg aaggcgggtt ctggcacctc aacatttgta agagcctgga
     4501 tgaagttgcc aagacgctct ccatttcaaa cgaagcagcc cggcatcggc ttgacggtgc
     4561 cagacaaaaa ctttttgcgg tgcgcgaaca gcgcgtacat ccggggcgcg atgaaaaaat
     4621 cctcgccagc tggaacgggc tgatgattaa aggcatggcg cgcgccgcac gggtttttgg
     4681 gcatgagaat tggctcgctt cggcgcagcg cgcctgcgat ttcgtccgca acgccttatg
     4741 gcgcgacggg cggctcctcg ccacttacaa gggcgcgccg ggcgccgacg caacaacgca
     4801 acattcaggc atatccgcca aaggaagcac cggaagagcg catctcaacg cctatctcga
     4861 cgattacgcc tttatgctcg atgcgctgct ggaactgctg cagacgcaat tccgcaaagc
     4921 cgacttcgat ttcgcgaaag acctcgccga agtcatgctt gagcaattcg aggaccgtga
     4981 gctgggcggt tttttcttca ccagccgcga ccatgaaaaa ctgattcatc ggccgaaagc
     5041 ggggcacgac aatgcgacgc cttcgggcaa cggcatcgca attttcgcgc tgcaacgcct
     5101 tggccatttg ctgggtgagc cgcgctatct tgaagccgca gagcgcgcat taaaactttt
     5161 ttatccggag gtaagcagcc gcccaagcgg ttacaccacc attgccatcg cgctggaaga
     5221 atttctcgcg ccgccacaga ccgtgattct gcgcggcgaa gcggaagcgg tgcgcgaatg
     5281 gcagcaggaa ctcggccggg aatacctgcc gcacgcgctg attctcgctt tgcccaaggg
     5341 ccttgccggc ctgccgcaaa ccttggaccg tgaagcaaag ggaaaagtca acgcttgggt
     5401 gtgccagggc gttaaatgcc tgccggctat caataagctg caagaactca agcaggtatg
     5461 caaaaccggt caggctggtt aaaatcgcgg acattttcaa ccaaggagca aacatgagga
     5521 cactattgat aggattactt tcgggcctgg ccttggccac ggcgagcgcc aatgcgctgg
     5581 ctgatgcaaa ggcaggagag gcgctggcaa agaaaaataa ctgcttcgcc tgtcacgcgg
     5641 tggacagtaa gctggtgggg cccgcttata aggacgtcgc caccaaatac aagggcgaca
     5701 aaagcgcgga agccaggctg attgagaaag tcaaaaaagg cggctccggc gtgtggggac
     5761 agattccgat gcctcccaat tccccccaag tgaaagacga agacatcaaa accatcgtgc
     5821 agtgggttct gtccctataa gcttccctgg ttaccggaaa agccggcgca aggccggctt
     5881 gtttgtttcc atcacgagcc atcgccgcaa attgacaagg tttaggtgac tgcggataat
     5941 cgtttgcatt tcatgaagct atgcatatgt caggagtaag catgaaattt agccacggtt
     6001 tcgcattggc cattctggca gtaactctcg gcctcttggg ttgtggcaag gagcaaacat
     6061 cttcatctga gctcgtcgtc aaaatcggct cggttgcccc cctgaccggt ccacaggcac
     6121 acctcggcaa agacaacgac aacggcgcgc gcctggcgat tgaggaagta aacgcgcgag
     6181 gcccggtgat tggcggcaag aaagtgcgct tcgagctcat cagcgaagac gaccaggccg
     6241 atccgaaaac cgctaccatc gttgcgcaga aactggtgga tcaaaagatc aacgcggtca
     6301 tcggccacct taactccggc gccactattc ccgcttccaa gatttaccac gacgccgacg
     6361 tggtgcagat ttccccctcc gccaccgcga ttccctatac gcagcaaggc ttcaagaatg
     6421 ccttccgggt catgactaac gatgaacagc aaggcaaagt gctgggtgaa ttcgcagtga
     6481 gccaaatggc cgcgaaaaaa atcgccatca ttgacgaccg caccgcttac ggccagggac
     6541 tggctgacca gttcgagaaa gcagcaaagg ccggcggcgc ggag
//
LOCUS       DATIOW010000014         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_207774, whole genome shotgun sequence.
ACCESSION   DATIOW010000014 DATIOW010000000
VERSION     DATIOW010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207774"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VLL03_00330"
     CDS             complement(<1..271)
                     /locus_tag="VLL03_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HSS45850.1"
                     /translation="MNVTQDLSLMGMVTHASVLVQLVILLLLVVSLMSWWYIFRKMFV
                     IKRATRQSNEFEKDFWSGKDLNSLYQSAASSRHSAGSLEKIFDAGF"
     gene            complement(379..1416)
                     /gene="ruvB"
                     /locus_tag="VLL03_00335"
     CDS             complement(379..1416)
                     /gene="ruvB"
                     /locus_tag="VLL03_00335"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HSS45851.1"
                     /translation="MIETDRLIAAAPLSPQEEALERALRPKSLSEYIGQEKIRGQLQI
                     FIEAARKRKESLDHVLLFGPPGLGKTTLAHIIARELGVNLRHTSGPVLERAGDLAALL
                     TNLEPNDVLFIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSVKLDLPAFTLIG
                     ATTRAGMLTNPLRDRFGIVARLEFYSAPELTQIVVRSARLLNVEIAHEGALEIAGRSR
                     GTPRVANRLLRRVRDYAEVRAKGKVTREVADAGLKMLDVAAIGLDVMDRKLLLAVMEK
                     FSGGPVGIDNLAAAIGEERDTIEDVLEPYLIQQGYLQRTPRGRMATANAYRHFGLALP
                     KNYATGDLWEG"
     gene            complement(1489..2064)
                     /gene="ruvA"
                     /locus_tag="VLL03_00340"
     CDS             complement(1489..2064)
                     /gene="ruvA"
                     /locus_tag="VLL03_00340"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HSS45852.1"
                     /translation="MIGRITGKLIEKRPPQVLLEVAGVGYELDVPMSTFYQLPAIGQQ
                     VTLHTHLIVREDAHLLYGFATERERGAFRQLLKISGVGARIALSVLSGLSVADLAQSV
                     TNQDSGRLTKIPGIGKKTAERLLLELRGKLETGMATGAAPAAGTGQDVLNALAALGYN
                     DREAAWAIKQLPEGLPVADGIRQALKLLSKA"
BASE COUNT          455 a          646 c          651 g          459 t
ORIGIN      
        1 ggaagcccgc atcgaagatt ttctccaggc tgccggcgct gtggcgtgag ctcgcggcgc
       61 tctgatagag cgaattcaaa tccttcccgc tccagaaatc cttttcgaac tcgtttgact
      121 gccgggtggc gcgcttgatc acgaacatct tgcggaagat gtaccaccag gacatcagcg
      181 acaccaccaa aagcaacagt atcaccagct gcaccaacac gctggcgtgg gtgaccatgc
      241 ccatcaagga cagatcttga gtgacattca cggttttttt cctaacgcaa ggtagttggc
      301 atagtagacc acgccgccgg tatcagtgtc ttcgtaataa atccgcaccg gctcggtgaa
      361 agttttgcgc tctgagattt agccctccca caggtcgccg gttgcataat ttttgggtag
      421 cgccagccca aagtgccgat aggcgttggc agtcgccatg cgtccgcgcg gggtgcgctg
      481 gagatagccc tgctggatga gataaggctc cagcacgtct tcgatggtgt cgcgttcttc
      541 gccgatagcc gcagcgagat tatcaatgcc caccggaccg ccgctgaatt tttccatcac
      601 cgcgagcagc aactttctat ccatcacatc gagacctatc gcagccacgt ccagcatctt
      661 gagtccggca tcggcgactt cgcgagtgac tttgcctttg gccctgacct cggcgtaatc
      721 gcgcacgcgc cgcagcaggc gattggcgac gcgtggcgtg ccgcgcgagc ggccggcgat
      781 ttcgagcgcc ccttcgtgcg cgatttccac gttgagaagc cgggccgagc gcaccacgat
      841 ttgcgtgagt tccggcgccg aataaaactc cagccgcgcg acgatgccga agcggtcgcg
      901 caagggattg gtgagcatgc cggcgcgggt ggtggcgccg attagcgtaa acgccggcaa
      961 atcgagcttc accgagcgcg ccgccggtcc ttcgccgatc atgatgtcga tttgataatc
     1021 ctcgagcgcg gggtagagga tttcctccac caccggcgag agacggtgga tttcgtcgat
     1081 gaacagcaca tcgttcggct cgagattggt gagcagcgcc gccaaatcac cggcgcgctc
     1141 cagcaccggc ccggaagtgt ggcgcaggtt cacgcccaat tccctggcga tgatgtgcgc
     1201 cagcgtggtc ttgcccaggc ccggcgggcc gaacagcaga acatgatcga gcgactcttt
     1261 gcgcttgcgc gccgcctcga tgaaaatctg cagctggccg cggatttttt cctggccgat
     1321 gtattcgctt aagcttttgg ggcgaagcgc ccgttccagc gcttcttcct ggggggaaag
     1381 cggtgcggcg gcaatcaggc ggtcggtctc gatcattgaa aagcggtgaa gggtgaaggg
     1441 tgaaaagtga agggtaaaac cggaagcaaa ttcatgttgt tttgctcctt acgcttttga
     1501 caataacttc aatgcctggc gtatgccatc ggcaacgggc aacccctcgg gcaactgctt
     1561 gattgcccaa gccgcttcgc ggtcattata gcccaaagcc gccagcgcat tgagcacgtc
     1621 ttgccccgtt cccgcagcgg gcgctgcgcc ggttgccatg ccggtttcca gcttgccgcg
     1681 taattccaga aggagacgct ccgcggtttt tttgccgatg cctggaattt tggtgagacg
     1741 cccgctgtcc tgattcgtga ctgactgcgc cagatcggca acgctcaagc cggagagcac
     1801 ggaaagcgcg atgcgggcgc cgacgccgct gattttcagc aactgcctga acgctccgcg
     1861 ctcccgctca gtggcgaagc cgtacagcag atgcgcgtcc tcgcgcacaa taagatgagt
     1921 gtgcaaggtt acctgctggc cgattgcggg caactggtaa aacgtgctca tcggcacgtc
     1981 gagttcatag cccaccccgg cgacctcgag cagcacttgc ggcggacgtt tttcgatgag
     2041 tttaccggtg atgcgtccga tcatttgagg cctctaaaaa acaagtattt tgtgtcaaga
     2101 caaggcgcgg ggttttttgc aagcgcagca tacaaagtag tatgtaagcg aagcaaaaaa
     2161 ccgagcaacg cagtattggc gcaaaagacg ccgttttgca gaaacctcac a
//
LOCUS       DATIOW010000015         4085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_4511, whole genome shotgun sequence.
ACCESSION   DATIOW010000015 DATIOW010000000
VERSION     DATIOW010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4511"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2292)
                     /locus_tag="VLL03_00345"
     CDS             complement(<1..2292)
                     /locus_tag="VLL03_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HSS45853.1"
                     /translation="MTNLFWILATVAAAWALAYHRAAGWAWSAGALVLAVVTLGAAPV
                     LSVLLWIAFAISIALNIPSIRMQYISAPLLEWFKKVLPQVSQTEQEALDAGTVWWDGD
                     LFSGNPDWKKLLALPRPQLTPEEQAFLDGPVEQLCAMLDEWRISHELYDMPQEVWQFI
                     KDQGFLGMIIPEQYGGKGFSALAHSAVITKLASRSSTATVSVMVPNSLGPAELLLHYG
                     TEAQKNYYLPRLAKGLEIPCFALTGPEAGSDAGSIPDYGIVCRGEFQGKEVLGMRVTW
                     EKRYITLGPVATLLGLAFKLYDPDHLLGQEANIGITLALIPTSTAGVNIGRRHFPLNA
                     MFQNGPNSGKDVFIPMDYIIGGQERVGHGWRMLMNCLAAGRSISLPASSTGMAKLASR
                     ATGAYSRVRVQFKTPIGKFEGIEEALARIGGNTYLMDAARVMTAGAVDLGEKPSVISA
                     IVKYHITERGRQVINDAMDVHGGKGICMGPNNYLGRAYQQIPVGITVEGANILTRSMI
                     IFGQGAIRGHPYVLKEIAATNEPDADKALSDFDSVLFGHIGFTLSNAARASFHGLTGA
                     RFAAAPGAPEIARYYQHLTRLSAAFALLADVAMLVMGGSLKRKEKLSARLGDVLSQLY
                     LSSSVLKRYEDEGRQTADLPLVRWSLQDALWRAQLALEGLLDNFPLGSWLWRRLVFPL
                     GRSFAPPSDDLGHAVASLLLSPSSVRNRLTAGMYLPVDEKDPVGRLEVALKAVMAAEA
                     VEAKIRTDTKAGRLKGKAPEELAA"
     gene            complement(2356..3063)
                     /locus_tag="VLL03_00350"
     CDS             complement(2356..3063)
                     /locus_tag="VLL03_00350"
                     /inference="COORDINATES: protein motif:HMM:NF037008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSS45854.1"
                     /translation="MKLQAVTIKQDSSTQERILDAAEKLFMERGFVATSLRMITAKAK
                     VNLAAVNYHFGSKEELIKAIFSRRLGPLNRERIAHLDALEQAAAGKPLTPEQILEAYV
                     SFALHLGRDPLQGGAVFLRLLGRVFAEPPGYMKTFLSEQYRGVVRRFKTAFTRALPHL
                     PEEELVWRMHFMFGVVAYTMAGNDALQLICNYTLDDSKSAGAVTRHLMPFLVAGLNAP
                     PPRLRAVKKTERVHEPA"
     gene            complement(3258..>4085)
                     /locus_tag="VLL03_00355"
     CDS             complement(3258..>4085)
                     /locus_tag="VLL03_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSS45855.1"
                     /translation="QVTHYCGAPVVHSMLISAPAEMKAGITHKISGLVAAAAPPAAMI
                     EGMERLGFNITHVYGLTETYGPASVCAKHPEWDKLDIGARTERNGRQGVRYTMQEGMT
                     VMDPETMRPVPWDGETMGEIMFRGNITMKGYLRNPKATEAAFAGDWFHSGDLAVMQPD
                     GYVKIKDRSKDIIISGGENISSLEVEDVLYRHPAVMAAAVVAQPDPKWGETPCAFVEL
                     KEGAKATEKEIIEHCRKSMARFKVPKKVIFGVLPKTSTGKIQKFVLRERAKSASAIE"
BASE COUNT          843 a         1248 c         1148 g          846 t
ORIGIN      
        1 cgcggcaagt tcttcaggcg ccttgccttt gaggcggccg gcttttgtgt cggtgcggat
       61 cttggcttcc accgcttccg ccgccatcac cgccttgagg gcgacctcta aacggccgac
      121 cgggtccttc tcatcaaccg gcaagtacat accggcggtc aagcggttcc ggacagagga
      181 aggcgagagc aggaggctgg ccaccgcgtg ccccagatca tcgctcggag gggcgaaact
      241 ccggccgaga ggaaacacca gccgccgcca tagccacgac ccaagcggaa aattatcgag
      301 caggccctca agcgctaatt gcgcgcgcca cagcgcgtcc tgcaaagacc atcgcaccag
      361 cggcaaatcc gccgtttgcc tgccttcgtc ttcataacgc ttcaatacgc tggaagagag
      421 ataaagctga ctcaacacat cgccgagccg cgccgaaagt ttttccttgc gctttaacga
      481 accgcccatt accagcatcg ccacgtccgc cagcagtgcg aaggccgcgc tcaagcgggt
      541 caaatgctgg taatagcgcg cgatttccgg ggcgcccgga gcggcggcga aacgcgctcc
      601 ggtcaggcca tggaacgacg cgcgcgccgc attactcaaa gtaaagccaa tgtgtccgaa
      661 caacacgctg tcgaaatcgc tcagcgcttt atcggcgtca ggctcattgg tcgcggcgat
      721 ttccttaagc acataaggat ggccgcggat cgcgccctgc ccgaaaataa tcatcgagcg
      781 cgtgagaatg ttcgcgcctt ccaccgtgat accgacggga atctgctgat aagcgcggcc
      841 cagataattg ttgggcccca tacatatgcc tttgccgccg tgcacgtcca tcgcatcgtt
      901 aatcacctgc cggccgcgct cggtgatatg gtatttgact atggcggaaa tgaccgatgg
      961 cttctctccc aaatccaccg cgcccgctgt catcacgcgc gctgcgtcca tcaaataagt
     1021 attgccgccg atgcgcgcca gcgcttcttc aatgccttcg aatttgccga tgggcgtttt
     1081 gaattgcacg cgcacccggg aatacgcgcc ggtagcgcgg gaagccagct tggccatgcc
     1141 ggtgctgctg gcggggagtg aaatcgagcg cccggcggcg aggcagttca tcagcatgcg
     1201 ccagccgtgg ccgacgcgct cctgcccgcc gatgatgtaa tccatcggaa tgaaaacgtc
     1261 cttgccggag ttgggcccgt tctggaacat ggcgttgagc gggaaatggc ggcgcccgat
     1321 attgacgccc gcggtgctgg tgggaatcag cgccagcgtg atgccgatat tcgcctcttg
     1381 ccccagcaga tgatcggggt cgtagagctt gaacgccaga cccaacagcg tcgccaccgg
     1441 ccccagcgtg atataacgct tttcccaagt cacgcgcatg cccagaactt ccttgccctg
     1501 gaattctccg cggcagacga tgccgtaatc ggggatcgag ccggcatccg agcccgcttc
     1561 cggtccggtc aacgcgaagc acggaatttc gaggcctttg gcgagacgcg ggagataata
     1621 atttttttgc gcctcggtcc cgtaatgcag cagcaattcc gccggcccca gggagttagg
     1681 caccatcacc gaaacggtgg cggtgctgct gcgggaagcg agtttggtaa tcaccgccga
     1741 atgcgccagt gcggaaaaac ccttgccgcc gtattgctcg gggatgatca tgccgaggaa
     1801 accctggtct ttgatgaact gccagacttc ctgcggcata tcgtagagtt catgggagat
     1861 gcgccactcg tcgagcatgg cgcacaactg ctccaccggc ccgtcaagaa acgcctgctc
     1921 ttcaggagtc agctgcggcc tgggcaaggc cagcaatttt ttccagtccg gattgccgct
     1981 gaataaatcg ccgtcccacc acaccgtacc ggcatcgagc gcttcctgct cggtctgcga
     2041 aacctgaggc agaacctttt tgaaccattc caggagcggc gcgctgatgt attgcatgcg
     2101 tatcgaagga atgttaagcg cgatggagat tgcaaacgca atccatagca gcacgctcaa
     2161 aacgggcgcc gcgccgaggg tcaccacggc gagaaccaga gcacccgcgc tccacgccca
     2221 gccggcagcg cgatggtaag ccagcgccca ggccgccgcc acggttgcga gtatccagaa
     2281 caagtttgtc atgatgagtt actcctttca aaaccccggt gcaaaccggg gtaaatcagc
     2341 ggacgcctgg ccttatcagg cgggctcgtg cacccgttcc gtttttttca ccgcgcgcaa
     2401 gcgcggcggc ggcgcattca agcccgctac caaaaacggc atgagatgcc gggtgacggc
     2461 tccggcgctt ttcgaatcat ccagcgtata gttgcaaatc aactgcagcg cgtcgttccc
     2521 cgccatggtg taggccacca cgccgaacat gaagtgcatg cgccacacca gctcttcctc
     2581 gggcagatgc ggcagcgctc tggtaaacgc cgttttgaag cgccgcacca ctccgcggta
     2641 ttgctccgac agaaaagtct tcatataacc cggcggttcg gcgaagactc tgcccaatag
     2701 tcgcaaaaac accgcgccgc cttgcaacgg atcgcgcccc aaatgcaacg cgaaagaaac
     2761 gtaagcttca agaatctgtt ccggtgtcag cggtttgccc gcggcggctt gctccagcgc
     2821 atcgagatga gcgatgcgct cgcggttaag cggcccgagc cggcgtgaga aaattgcctt
     2881 gatgagctct tctttggaac cgaaatggta attgacggcg gcaagattga ctttcgcctt
     2941 ggcggtaatc atgcgcaggg aggtggcgac aaacccgcgc tccatgaaca gtttctcggc
     3001 cgcgtcgagg attcgctcct gggtgctgct gtcctgtttt atggtgacgg cttgtagttt
     3061 cacggcttaa gtctttacgt taatattttg ctttatatta ttgaatataa aagataatat
     3121 tattaaagaa acggctatca ggggttaaca gaatttatgg atttaggggg atccaaattt
     3181 caaacagtcg tttgaatcct gctcctgttt tacgcgaatg tcaacgctaa cgtaaaggcc
     3241 aaaaatccat gatgaactta ttctattgcc gatgcggact tggcgcgctc gcgtaaaaca
     3301 aatttctgta tcttgccggt cgaggtcttg ggcagcacgc cgaatatcac ttttttcggc
     3361 accttgaagc gcgccatgct ttttcggcaa tgctcgatga tttccttctc ggtggctttg
     3421 gcgccttcct tcaattccac gaacgcgcac ggcgtctcgc cccatttcgg gtcgggttgc
     3481 gccaccaccg ccgccgccat caccgccgga tggcgataca gcacgtcctc gacttccagc
     3541 gaggaaatgt tctcgccgcc ggagatgata atgtctttcg agcggtcctt gattttcaca
     3601 tagccgtcgg gctgcatcac cgccagatcg ccggagtgaa accagtcgcc ggcaaacgcc
     3661 gcctcggtgg ctttcggatt tctcagataa cctttcatgg tgatgttgcc gcggaacatg
     3721 atttcaccca tggtctcgcc gtcccacggc accggccgca tggtttccgg atccatcacc
     3781 gtcatgcctt cctgcatggt atagcgcacg ccttgccggc cgtttctttc ggtgcgcgcg
     3841 ccgatatcga gcttgtccca ttccggatgc ttggcgcaca ctgaagccgg cccataggtt
     3901 tcggtgaggc cgtaaacgtg ggtgatattg aagcccagac gctccatgcc ttctatcatc
     3961 gccgcgggcg gcgcagcagc ggccaccagg ccgctgatct tgtgcgtgat gcccgccttc
     4021 atctccgcgg gcgcgctaat caacatgctg tgcacaacgg gcgcaccgca gtaatgcgtc
     4081 acctg
//
LOCUS       DATIOW010000016         6048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1529739, whole genome shotgun sequence.
ACCESSION   DATIOW010000016 DATIOW010000000
VERSION     DATIOW010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529739"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VLL03_00360"
     CDS             complement(<1..562)
                     /locus_tag="VLL03_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS45856.1"
                     /translation="MTLLAGFLSILFHALLLIGMSAAVGGLIFLLAVLLPLANTGNAA
                     RLAIRRSLIVLVWGAALLALGQVLGLLMELWAIADESGEWPIGAFFSTGFAHAGLLHI
                     AFALALASSGVWLIKQPASRGAWTASIAAAILLMISGAWLVHAVSRLEGMVPLMVMTI
                     LHQLGAAVWVGGIIHLVSLWRQTRNGA"
     gene            complement(566..934)
                     /locus_tag="VLL03_00365"
     CDS             complement(566..934)
                     /locus_tag="VLL03_00365"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HSS45857.1"
                     /translation="MKKKLSAVALVLFFFLNGCGLALAHAVVVESSPRESAVLEQAPD
                     RIQLRFNVKIEKALARVSLTKGGKQKIKLPPSDFSQSAPERLEVLLPPLEPGDYLLRY
                     SVLAADGHTTQGALRFSIAR"
     gene            complement(1079..2071)
                     /locus_tag="VLL03_00370"
     CDS             complement(1079..2071)
                     /locus_tag="VLL03_00370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HSS45858.1"
                     /translation="MKLGIAIAALAGLLAAVLLIGYHGFIAVSDILLTAGWGLVVVSL
                     FHLLPVTLSSLAWRRLLRSTWRHSLPVFFWARSVREAVGSLLPVMQVGGEFVGARILT
                     FHGANAGIAGASVVVDLTLETIAQFFFTLLGLLLLVLLGSSEQTVRWAAAGLAVAALA
                     LAGFVFAQRKGMFKLLENFIEKITDKWEWLSLGKIDNLHDTIQKLYEDRYTLLIAGVT
                     HFLSWIISAGEVWLALYFMGNPVSFLEVLILESLSHAVKSAAFAVPGALGVQEGVYML
                     LGEVLGLSPEMGLALSLVKRVRDLLLGIPALLGWQAVESGRLWFRTNRNKSDGR"
     gene            complement(2068..4692)
                     /locus_tag="VLL03_00375"
     CDS             complement(2068..4692)
                     /locus_tag="VLL03_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HSS45859.1"
                     /translation="MLDSFGNQYRRVVVRLVEFSAHAAKGLVAIVLLLTAVFLFYTAT
                     HLSLDTDPTEMLDPQLRFRQLEIDLQKAFPQFSDVIVVVIEGDSAGHADSASQKLIAQ
                     LKQETKALESIYDPEQTSFFSQNGLLYLDKKELLQLSDRLTEAAPFLGTLAQDPSLRG
                     LFTVLGRALDENLSPDYRTRLKHMLDSISGIAEALSAGRLLKQSWRGQFIEGTMAQGN
                     DRRHFILIKPKFDYSSLQPGQEAINTVRRVAQELQLDPQLGVRVRLTGDVPIEDEELD
                     SVARGAGLASLFSTLLVCVILFLGLRSTRLVAAILITLFVGLIWTGAFTAFAIGHLNL
                     ISVTFAVLFIGLGVDFGIQFGMRYKEELHRTKDRENALSEAAYGAGGMLTLAAVAAAI
                     SFFSFTPTSYRGLAELGVISGVSMFIALISNLTLLPALLKLMPVDMESKLPKSSMLTR
                     LRFPVRRHRKGILLAAMLAGVVSLALLSQVRFDFNPLNMKDPNTESVATFLDMLNDPN
                     TTPYTIDVLTTNLNQAKEVAAKLEKLAVVDKTVTLASFVPDDQDEKLAVIADMALVLQ
                     PLVTPTRVAAPSAQEEFQAVEQFQKKLQETSGKNPDSAFAASMKRLANALEKLKSVPG
                     WPDQTLKQLRDGVVGDLPQNLAQLRGLLQPQRIELKDVPQDLRERYLAADGRARVEVF
                     PKINVTDNKELRRFVYEVQKVAPHAIGTPVALVEGGDTVVQACLLATGIALFAALILL
                     LLVLQSVSGAFLVLLPLVLAMVMTAASSVILDLPLNLANIIALPLLLGLGIAFGIYLV
                     MRKRSGLDVEHLFRSSTPRAVLFSALTTVVSFGSLAFSEHRGMAGMGLLLTLALSFAL
                     LCTLVVLPAIMSVIEDQQ"
     gene            complement(4751..5620)
                     /gene="hpnK"
                     /locus_tag="VLL03_00380"
     CDS             complement(4751..5620)
                     /gene="hpnK"
                     /locus_tag="VLL03_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013428742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis-associated protein HpnK"
                     /protein_id="HSS45860.1"
                     /translation="MKRLIVTADDFGASLPVNEAIEQAHRQGILTTASLMVGAAAARD
                     AVERAKRLPSLKVGLHVVLVRGRPLLPPDLVPDLVDAQGNFSSQLARASVNFFFRSKV
                     RRQLEAEIRAQYEAFRATGLVLDHVNAHNHMHLHPTVLGLILKIGQGYGAHAVRLPNE
                     PFIYSWRAARNAFTLRLANSLLLKPWLTLMRWRLNKAGVACNEYMFGMSDSGQMNEQR
                     VLGLLAHLPHGVSEMYFHPALEAWDSVDPGMAHYDHQAEFQALVSPAVARAVRQHQIE
                     KISFGDLPAKPFS"
     gene            complement(5617..>6048)
                     /locus_tag="VLL03_00385"
     CDS             complement(5617..>6048)
                     /locus_tag="VLL03_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HSS45861.1"
                     /translation="CRKLGVVVHGTFIMGLPGETHETIEETIRFAKEINPHTIQVSLA
                     AAYPGTYLHKQATENGWLLRDPTHIVAQEGFQVSSLNYPHLSHEEIFKALEKFYKRFY
                     FRPSKIAEITFEMMQSWEMTKRRLREGVEFFRFLYGREDRV"
BASE COUNT         1351 a         1774 c         1793 g         1130 t
ORIGIN      
        1 cggcgccatt gcgcgtttgc cgccacagcg agacaagatg aatgatgccg cctacccaca
       61 ccgcagcgcc cagttggtgc agtatcgtca tcaccatcaa tggcaccatg ccttcgaggc
      121 ggcttaccgc gtgcaccagc caggcgccgc ttatcatcag gagaatcgca gcggcgatgg
      181 atgcagtcca ggcgccacgt gatgccggtt gtttgataag ccatactccg cttgaagcca
      241 aagcaagggc aaatgcgata tgcaacagcc ccgcgtgggc gaatcccgtg gaaaaaaacg
      301 cgccgatggg ccactcgccc gactcatccg cgatcgccca gagctccatc agcaatccca
      361 gaacttgccc gagcgcaagc agggcggcgc cccagaccag aacgatgagc gaacgcctta
      421 tcgcaagccg tgccgcgttg ccggtgttgg caagcggcag caacacggcg agcagaaaaa
      481 tcagcccgcc taccgccgcc gacatgccga tcagcaacag tgcatgaaac agaatcgata
      541 gaaaaccggc aagcagagtc atgtgttatc tggcaatact gaagcgcaat gccccttggg
      601 tagtatggcc gtccgcagcc agcaccgaat agcgcagcaa gtaatccccc ggttccagag
      661 gcggcaaaag cacttccagt ctttccggcg cgctttggct gaaatcggag ggcggcagtt
      721 taatcttttg cttgccgcct ttggtgagac tcacacgggc gagcgctttt tcaattttga
      781 cgttaaaacg cagttggatg cggtccggcg cctgttcgag caccgcgctt tcgcgcgggc
      841 tggattcaac cacgacggcg tgcgccaagg caaggccgca cccatttaga aaaaagaata
      901 ggaccaaggc gacggcagac agtttttttt tcatgattgc gaaatttgtg taatgcgcac
      961 gaatcatgcg caactaagca aacctggcaa gaatgcccgc aaaacacgaa tattttacca
     1021 gcttgggtcg caattcaaaa taccgctacg ccattttccg gtgaaatgag aacttctctc
     1081 aacgtccgtc gcttttgttg cggtttgtcc tgaaccacag gcgcccgctt tccaccgcct
     1141 gccatcccag cagcgcgggg atgcccagca acaaatcacg cacccgtttc accagggaca
     1201 gcgccagccc catttccgga gaaaggccca acacttcgcc cagcagcatg tacacgcctt
     1261 cctgcacgcc cagagcgccg ggaactgcaa aagcggcgct ttttaccgcg tggctcaaac
     1321 tttccagtat cagcacttcc aggaaactta ccggattacc catgaaataa agcgccagcc
     1381 agacctcgcc tgcgctgatg atccaggaca gaaaatgtgt cactccggcg ataagcagtg
     1441 tgtagcggtc ctcgtagagt ttctggatgg tgtcgtgcag attgtcgatt tttcccagcg
     1501 acagccattc ccatttgtcg gtgatttttt caataaaatt ttccagcagc ttgaacatcc
     1561 ccttgcgttg ggcgaaaaca aaaccagcca gagccagcgc ggcgacggcg aggcccgctg
     1621 ccgcccagcg caccgtctgt tccgaactgc ccagcaatac cagcagcagc agcccgagca
     1681 gagtgaaaaa gaattgcgcg atggtttcga gcgtcagatc caccaccacg ctggcaccgg
     1741 cgatgccggc attggcgccg tggaaggtaa ggatcctcgc gccgacaaac tcgccgccga
     1801 cttgcatgac cggcagcaaa ctgcccacgg cttcgcgcac cgagcgcgcc cagaaaaaca
     1861 ccggcaagct gtgacgccag gtgctgcgca gcaaacggcg ccaagccaat gaagaaagcg
     1921 tcaccggcag cagatgaaac aggctcacca ccaccagtcc ccagccggcg gtgagcagga
     1981 tatcggatac cgcgataaag ccgtggtagc cgattagcag aaccgccgcc agcagaccgg
     2041 cgagcgcggc gatggcgatg ccgagcttca ctgctggtcc tcgataacgc tcatgatcgc
     2101 cggcaaaacc accagggtgc agagcagcgc gaacgacaaa gcgagggtga gtaaaagacc
     2161 cataccggcc atgccgcggt gttcggagaa agccagcgag ccgaacgaaa ccacggtggt
     2221 aagcgcgctg aacaacacgg cgcgcggtgt gctgctgcga aacaggtgct cgacgtcgag
     2281 cccgcttctt ttgcgcatca ccaggtaaat gccgaaggct atccctaagc ccagcagcag
     2341 cggcagcgcg atgatgttcg ccaggttaag cggcaggtcg agaatcacgg aacttgctgc
     2401 ggtcatcacc atcgccagaa ccagcggcag caacaccaga aacgccccgg agacgctttg
     2461 caataccagg agcagcagga ttaacgctgc gaacaaggcg atcccggtgg ctagcaggca
     2521 ggcctgcacc actgtgtcgc cgccttcgac cagcgccacc ggcgtgccga tggcatgggg
     2581 cgcgactttt tgcacctcgt acacaaagcg gcgcaattcc ttgttgtcgg ttacattgat
     2641 tttgggaaaa acctcaaccc gggcacgacc gtcggccgcg agatatcttt cccgcaaatc
     2701 ctgcggcaca tccttgagct cgatgcgctg cggttgcagg agcccgcgca actgcgccag
     2761 attttgcggc aagtcgccta ccacgccatc gcgcagttgc ttgagcgtct ggtccggcca
     2821 gccggggacg gatttgagct tttccagagc gttggccagg cgcttcatgc ttgcggcaaa
     2881 agcggaatcg ggatttttcc cactggtttc ctgcaatttt ttctgaaact gttccacggc
     2941 ttggaattct tcttgcgcgc tcggcgcggc aacacgcgtc ggtgtaacca gcggttgcag
     3001 caccagcgcc atgtcggcga tgaccgcaag cttttcgtcc tggtcgtcgg gcacgaaact
     3061 ggcgagtgtc accgttttgt ccaccaccgc gagcttctcc agttttgccg cgacctcctt
     3121 ggcctggttg aggttggtgg tgaggacatc aatggtgtag ggcgtggtat tggggtcgtt
     3181 cagcatgtcg aggaaggtgg cgaccgactc ggtgttagga tccttcatgt tgagggggtt
     3241 gaagtcgaag cgcacctgtg acagcagcgc cagcgaaacc acgcccgcca gcatagccgc
     3301 gaggagaatg cctttccggt ggcgccgcac cggaaagcgc aagcgggtga gcatgctgct
     3361 cttgggcaat ttgctttcca tgtccaccgg catgagcttg agcagcgccg gcaacagagt
     3421 aagattggaa atcagggcga tgaacatgct cacgccggaa ataacgccaa gctcggcaag
     3481 cccgcggtaa ctagtggggg taaaggagaa aaagctgatg gcggcggcga ccgccgccag
     3541 cgtcaacatc ccgcctgcgc cataggcggc ctcgcttaac gcgttttcgc gatccttggt
     3601 gcgatgcagc tcttccttgt aacgcatgcc gaactgaatg ccgaaatcga cacccaggcc
     3661 gatgaacagc acggcgaagg tcactgaaat caaattaaga tggccgatgg caaaagcggt
     3721 aaaagcccca gtccaaatca ggccgacaaa cagcgtgatc agaatcgcgg ccacgagtcg
     3781 ggtcgagcgc agccccagga acaaaatcac gcacaccagc aaagtggaga acaggctggc
     3841 caggcccgcg ccgcgcgcaa cgctgtccag ctcttcatcc tcgataggaa cgtctccggt
     3901 gagacgcacg cgcactccaa gttgcggatc gagctgcaat tcctgtgcga cccggcggac
     3961 ggtgttaatt gcctcttggc ccggctgcag cgaggagtaa tcaaatttag gtttgatgag
     4021 aatgaagtgc cgccggtcgt tgccttgggc catggtgccc tcgataaact ggccacgcca
     4081 ggattgcttg agcaggcgcc cggcggacag agcctcagca atgccgctga tgctgtccag
     4141 catgtgcttg aggcgggtgc ggtagtcggg actcagattt tcatcgagtg cccggccaag
     4201 cacggtaaac aggccgcgca agctggggtc ctgcgccagc gtgccgagaa atggcgccgc
     4261 ctcggtcagg cggtcggaaa gctgcagcaa ctcctttttg tcgagataaa gcagcccgtt
     4321 ctgggaaaag aaactggttt gctcggggtc ataaatcgat tcgagcgcct ttgtttcctg
     4381 ctttagctgc gcgataagtt tttgcgaggc tgaatcggcg tgtccggcgc tgtcgccttc
     4441 aatcaccact acaatcacat cggaaaactg cgggaaggcc ttctgcaggt ctatttccag
     4501 ctggcggaag cgcagctggg gatcgagcat ttcggtggga tcggtgtcga ggctcaagtg
     4561 agttgcggtg taaaagagaa acaccgcggt caacaacaag acgatcgcca ccaagccttt
     4621 ggcggcgtgg gcagagaact ccaccaggcg caccacgacg cggcgatact gattaccaaa
     4681 gctgtctagc aagagccgtc cccgttcgtg gaagcgaagc tgcgttgtga cttgccggaa
     4741 ttcatgcgat tcaagaaaaa ggtttggccg ggagatcgcc gaaactgatt ttttctatct
     4801 ggtgctggcg cacggcgcgc gcgaccgcgg ggctcaccaa ggcctggaac tcggcttgat
     4861 ggtcatagtg cgccatcccg gggtccacgc tgtcccaagc ttcaagcgcc ggatggaaat
     4921 acatttccga gacgccgtgc ggcagatggg caaggaggcc aagcacgcgc tgttcgttca
     4981 tctggccgga gtcgctcatt ccgaacatgt attcgttgca agccaccccg gctttattca
     5041 agcgccagcg catgagggtt aaccagggct tgagcagcag cgaattggca agacgcagcg
     5101 tgaacgcatt tcttgcggcg cgccaggaat aaatgaaagg ctcgttaggc aggcgcaccg
     5161 cgtgcgcgcc gtagccctgt ccgattttga ggatgaggcc gagcaccgta ggatgcagat
     5221 gcatgtggtt gtgggcgttg acgtgatcta gcacgaggcc ggtggcgcgg aacgcttcat
     5281 actgcgcgcg gatttccgcc tcgagctggc gtcttacctt ggagcgaaag aagaaattca
     5341 cgctggcgcg ggcgagctgc gaggaaaaat tcccctgcgc gtcgaccagg tcgggaacca
     5401 ggtcgggagg taaaagcgga cggccgcgga cgaggaccac atgcaagccg actttgagcg
     5461 agggcaggcg cttggcgcgc tccaccgcat cccgggctgc tgcggcaccc accatcaagc
     5521 tcgcggtggt gagaatgccc tggcggtgtg cctgctcgat tgcctcgttg acgggaaggg
     5581 atgcgccgaa gtcgtcagcc gtgacgatga gccgcttcaa acccgatctt cccgcccgta
     5641 aagaaagcga aaaaattcca cgccttcgcg cagccggcgc ttggtcatct cccagctttg
     5701 catcatttca aacgtgattt cggcgatctt gctcgggcgg aagtagaagc gcttgtagaa
     5761 tttttccagc gccttgaaaa tttcctcatg cgagaggtgc gggtagttga gcgagctgac
     5821 ctgaaagcct tcttgcgcga cgatatgcgt gggatcgcgc agcaaccagc cgttttccgt
     5881 cgcctgcttg tgcagataag ttccgggata ggcggcggcc agcgagacct gaatcgtgtg
     5941 cgggttgatc tccttggcga agcggatagt ctcttcaata gtttcgtgcg tctcgcccgg
     6001 cagccccatg atgaaagtgc cgtgcaccac cacgccgagc ttgcggca
//
LOCUS       DATIOW010000017         8084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1936342, whole genome shotgun sequence.
ACCESSION   DATIOW010000017 DATIOW010000000
VERSION     DATIOW010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8084
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1936342"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1111
                     /locus_tag="VLL03_00390"
     CDS             134..1111
                     /locus_tag="VLL03_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSS45862.1"
                     /translation="MISYIIRRIAYAVPILIGVNIITFTLFFVVNTPDDIARMQLGVK
                     RVTPEANAKWKQDHGYDQPLLFNSGAEGTSKLTKTIFYEKSVRMFVFDFGPADDGRDI
                     AHEIKTRMGPSLAIAVPVFLVGLMINITFALLIAFFRATYIDFWSVVLCVAMMSISGL
                     FYIIGGQYLVSKLWNLVPISGFAYGFDSIKFVVLPVVVGVIGGVGSGTRWYRTIFLEE
                     INRDYVRTARAKGLSELRVLFKHVLKNALIPILTGAVVVIPLLFMGSLITESFFGIPG
                     LGSYTIDAISSQDFAVVRAMVFIGSVFYIVGLILTDISYTLVDPRIRLE"
     gene            1159..2604
                     /locus_tag="VLL03_00395"
     CDS             1159..2604
                     /locus_tag="VLL03_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSS45863.1"
                     /translation="MLTPLWTDWLIYLLLALIALFVWQVRGRPHLVAPWRRVAQSKSG
                     VVAATVMAAYLIVGLLDSVHFRLPLENSNGDGERVYSVEALSIFDVLVTSLRTNKEKT
                     YSAPMATQLFAKEVMQLPDGREMRDYPWLKYGGAHLRNPDKEQAWDVTWRILVGVILA
                     VSCWLLAAALPVWLLARRARNSFRATAMALWRGQSEIPWSVVLITLGVLLLMVFPLGL
                     LCTHYHVLGTDKVGQDVLYLSLKSIRTGLVIGTLTTLVMLPFALAMGIMAGYFRGWVD
                     DVIQYIYTTLSSIPDVLLIAAAVLAMQVFMDTHPQLFPTITERADFRLLFLCIILGVT
                     SWTSLCRLLRGESLKLRELEYIQAAHAFGVSHLRIMTRHILPNVMHIVLITVVLGFSG
                     LVLAEAVLSYVGVGVDPSMISFGNMINGARLEMAREPIVWWSLFAAFLFMFVLVLAAN
                     LFADAVRDAFDPRVRTATKTFTAMNAKHAKA"
     gene            2752..4770
                     /locus_tag="VLL03_00400"
     CDS             2752..4770
                     /locus_tag="VLL03_00400"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSS45864.1"
                     /translation="MINNMPLLRIENLKTWLDNDGAPARAVDGLSLTLERGETFALLG
                     ESGCGKSMTALSIMRLLPDVGRIVSGEVLLEGQNLLALPEVAMRTVRGRRVAMIFQEP
                     SLSLNPVLTVGEQIAEVLERHKGMSGAQAKKRILELLDAVGIPDSSRRYREYPFQLSG
                     GMKQRVMIAIALAGEPDLLIADEPTTALDVTIQAQVLDLIRDLQKRTGMAILLITHDL
                     GVVSEMAHKVAVMYAGQIVETAQAREFFANPAHPYSRKLFASLPHSNKRTGKLEIIKG
                     TVPPLTRDFQGCRFAERCDFAWDKCRKIDPGWSEAACDHTVRCHLYSEGKAAKVEGSA
                     PAAGYKITQTNEPVADLLDVSGLKVYFPVHKGLFKRVVGYVKAVDGVTLEIKKGRTLA
                     LVGESGCGKTTVGKGILQLVPVTSGSVRYNGIDLTRLSRSDLRSRRSEFQIIFQDPYS
                     SLNPRMRVSEIIEEGMTALGIAASAEERAKRGDVLLEQVGLSPEMKSRYPHEFSGGQR
                     QRIAIARALAVNPKLIICDEPTSALDVSVQAQILNLLKELQDRLGLAYLFITHNISVV
                     EYLAHEVAVMYLGRIVETGTVEEVLNNPKHPYTRALLSAVPVIDKSGKREIIRLKGDL
                     PSPLNPPSGCHFHPRCPDVMPVCKESYPGDSRFSSTHCARCHLYPTGN"
     gene            complement(4818..5588)
                     /gene="gloB"
                     /locus_tag="VLL03_00405"
     CDS             complement(4818..5588)
                     /gene="gloB"
                     /locus_tag="VLL03_00405"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HSS45865.1"
                     /translation="MSNITVVPVPAFRDNYIWVIRNQHCAAVVDAGDATPVLAYLERE
                     KLELVSILCTHHHADHVGGNSGLLQRFRVPVYGPRHESISTLTHRLVEGDRVTVPQLG
                     LEFTVLDIPGHTAGHIAYYGANLLFCGDTLFACGCGKLFEGTARQMYTSLQKLVALPD
                     ETLVYCGHEYTLANIRFAKAADPDNPALIERETRACALREQGLPTVPSDIRLEKATNP
                     FLRCNHPQIIKTATRAAGKSLNDPVAVFAAVREWKNRF"
     gene            5617..6369
                     /locus_tag="VLL03_00410"
     CDS             5617..6369
                     /locus_tag="VLL03_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSS45866.1"
                     /translation="MSTTLSDWFETPLGQYLLEQEQTYFDKTVADVFGFNAVQLGLAH
                     HDFLRMSRIPLRVSAGPDIEARVKLDFPLLPFATNSIDLLMLPHVLEFSDNPHQILRE
                     AERVLLPEGQLIICGFNPRSLWGLSRQFGQSSGEYPWCGRFISLPRVKDWLALLGFEM
                     TAGRLGCYVPPFRQEKWLRRFRFLEAAGDRWWAIAGGVYFLQAIKRVQGLRLIKPNWN
                     EALRPKKSLAPVRRESKAKVLQFKTRRPSPSI"
     gene            6366..6821
                     /gene="rnhA"
                     /locus_tag="VLL03_00415"
     CDS             6366..6821
                     /gene="rnhA"
                     /locus_tag="VLL03_00415"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HSS45867.1"
                     /translation="MMAETVEIYTDGACKGNPGVGGWGALLKYSGHVRELFGGEAQTT
                     NNRMELTAVIEALSALKRPCNVKLHTDSRYVQQGISEWIHDWKKRNWRSADKKPLKNS
                     DLWKRLDQLAAGHQIEWIWVQGHSGDEGNEHADSLANRGVESMQLKREE"
     gene            6825..7532
                     /gene="dnaQ"
                     /locus_tag="VLL03_00420"
     CDS             6825..7532
                     /gene="dnaQ"
                     /locus_tag="VLL03_00420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HSS45868.1"
                     /translation="MTRQITLDTETTGLEPNLGHRVVELAAVELLNRRLTGNRFHSYL
                     NPERDSDAAALQIHGLTREFLQDKRRFKDIVNEFLEYISGAELIIHNAAFDEAFLDYE
                     LGLLGFKGLKEYCAGIVDTLKMAKELHPGKKNSLDALCERYEVDNSKRTLHGALLDAE
                     LLADVYVAMTRGQESLMMDEEDGALPRAEVAHAIKRDLLIITASPEELQAHAEQLQEI
                     DKASGGDCVWNKIAQAR"
     gene            7574..>8084
                     /locus_tag="VLL03_00425"
     CDS             7574..>8084
                     /locus_tag="VLL03_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HSS45869.1"
                     /translation="MSKILNEVLAANKEYSKNFGDKGKLPIPPARHFAVLTCMDARLQ
                     PSRFAGLAEGDAHVIRNAGGRASDDAIRSLVISYKLLGTREWFVIHHTDCGMETFTDE
                     VMRWLLANSLKTASLTKDGWRDSGKGPGSMEGQFIDWLTIKDQPKSMCADVARIRNHP
                     LVPRDIPIYG"
BASE COUNT         1697 a         2155 c         2508 g         1724 t
ORIGIN      
        1 aaaagcgacg aatgccccct ctccggcgcc ctcatccaat ccttctcccg gagggagaag
       61 ggacgaacgt tctctccccc tccgggagag agatagagtg agggatcccg ggagagggat
      121 tgggtgaggg atcgtgatta gctacattat ccgccgcatc gcctacgccg ttccgatcct
      181 gatcggggtg aacatcatta ctttcactct ttttttcgtg gtcaacacgc ccgacgacat
      241 cgcgcgcatg cagctgggag tgaaacgcgt cacgcccgaa gcgaatgcca agtggaaaca
      301 ggaccacggc tatgaccagc cgctcttgtt taattcaggc gcggaaggta cgagtaagct
      361 gaccaagact attttttatg aaaaatcagt gcggatgttc gtgtttgact ttggcccggc
      421 cgacgacgga cgcgacatcg cgcatgaaat caagacgcgc atgggtccca gcctcgccat
      481 agccgttccg gtgtttctgg tcggactcat gatcaatatc acgttcgcgc tgctgattgc
      541 gtttttccgc gccacctaca tcgatttctg gagcgtggtg ctgtgcgtgg cgatgatgtc
      601 gatttcgggg ctgttctaca tcatcggcgg ccagtatctg gtgagcaaat tgtggaacct
      661 ggtgccgata tcgggatttg cctatggttt tgactcaatc aaattcgtcg tcctgccggt
      721 ggtggtgggg gtcatcggcg gtgtcggcag cggcacgcgc tggtaccgca ccattttcct
      781 cgaggaaatt aaccgcgatt acgtgcgcac cgcgcgcgcc aagggcttgt cggaattgcg
      841 cgtgctgttc aagcatgttt tgaaaaacgc actgatacct attctgactg gagcagtggt
      901 ggtgatcccg ctcctgttca tgggcagtct gatcacggaa tccttcttcg gtattccggg
      961 gctgggcagc tacaccattg atgccatcag ttcgcaggat ttcgcggtgg tgcgcgccat
     1021 ggtgttcatc ggctcggtgt tttacatcgt cggactgatt cttaccgaca tttcctatac
     1081 tttggttgat ccacggataa ggctggagtg agggcgtgaa tgaagtgaag aggtgaagag
     1141 ggaaagacga tgttgtttat gcttacacct ttatggactg actggctgat ttacctgctg
     1201 ctggccctca tcgcgttgtt tgtctggcag gtgcggggaa ggccgcatct ggtcgcgccg
     1261 tggcggaggg tggcgcaaag caaaagcggg gtggtggcgg ctaccgtcat ggccgcctat
     1321 ctcatagtgg gtctgcttga ctcggtgcat tttcgcctgc cgctggaaaa tagcaatgga
     1381 gacggggagc gtgtttattc agtggaagcc ttgagcatat ttgatgtgct ggttacctcg
     1441 cttcggacca acaaggagaa aacctattcg gcgccgatgg cgacgcagct cttcgccaaa
     1501 gaagtgatgc aactgccgga tggcagggaa atgagggatt atccgtggct gaaatatggc
     1561 ggcgcgcatt tgcggaaccc cgacaaggaa caagcttggg atgtgacctg gcgcattttg
     1621 gtgggcgtga ttctcgccgt gtcatgctgg cttttggccg ccgcgcttcc ggtctggctg
     1681 ctggcccggc gcgcgcgcaa ttcttttcgc gcaacggcga tggccttatg gcgcggccaa
     1741 agtgaaattc cctggagtgt cgtcttgatt acgctgggcg tactgctgct gatggttttt
     1801 ccgctggggc tgctgtgcac ccattatcac gtactgggca ccgacaaagt ggggcaggat
     1861 gtgctctatc tttcgctcaa gagcatacgc accggcctgg tgataggcac gctgactacc
     1921 ctggtgatgc tgccgtttgc gctggcgatg ggtatcatgg cggggtattt caggggctgg
     1981 gtggatgacg tgattcaata catttacacc accttgagct cgattcccga tgtgctgctg
     2041 attgcggcgg cagtgctggc catgcaggta ttcatggaca cgcatccgca gctttttccc
     2101 accatcaccg aacgcgccga tttccgcttg ctgtttttgt gcatcatcct gggcgtgacc
     2161 agctggacca gcttgtgccg gctgctgcgc ggcgaaagcc tgaaactgcg cgagctggaa
     2221 tatatccagg cggcgcacgc ttttggcgtc tcccatttgc gcatcatgac ccggcacatt
     2281 ctgcccaatg tcatgcacat cgtgctgatt accgtggtgc tgggcttcag cggactggtg
     2341 ctggcggaag cggtgctgtc ttatgtgggg gtgggcgtcg atccctcgat gatcagtttc
     2401 ggcaacatga ttaacggcgc tcggctggaa atggcacgtg agccgatagt ctggtggtcg
     2461 ctgttcgccg cgttcctgtt catgttcgtg ctggtgctcg ccgccaatct tttcgccgac
     2521 gcggtgcgcg atgcgttcga cccgcgcgtg cgaacggcaa caaaaacatt caccgccatg
     2581 aacgcgaagc atgcaaaagc atgaaaaccg tttaccgcag agggcgcaga ggacacggag
     2641 aaattcaaaa atcaaggtta attcgattat ttgattttac aagatttgaa tttattaaat
     2701 caaaatgatt ttcctccgcg ctctccgcgc ccttcgcggt taaagagttt agtgataaac
     2761 aatatgccgc tattacgaat cgagaatctc aagacctggc tggataacga cggggcgccg
     2821 gcgcgtgcgg tggacgggct gtcgctgacg ctggagcggg gagaaacctt tgcgcttctc
     2881 ggcgaatccg gctgcggcaa atccatgacc gcactttcga tcatgcgcct gttgcccgac
     2941 gtcggaagaa tcgtttccgg tgaagtgctg ctggagggac aaaatctgct ggccctgccg
     3001 gaagtcgcca tgcgcacggt gcgcggccgg cgcgtggcca tgatattcca ggagccgagc
     3061 ttgagcttga acccggtgct cacggtgggc gagcagattg ccgaagtgct ggagcggcac
     3121 aagggcatga gcggcgctca agcgaaaaaa cgcatcctcg agcttctcga cgcggtgggg
     3181 atacccgata gctcacggcg ctatcgtgaa tatccgttcc agctctccgg cggcatgaag
     3241 cagcgggtga tgattgcgat tgcattggcg ggagaaccgg atttgctgat agccgacgag
     3301 ccgaccacgg cgctggatgt cacgattcag gcgcaggtgc tcgacctcat acgcgatttg
     3361 caaaagcgaa ccggcatggc gattctgctg attacgcacg atctgggcgt ggtttccgaa
     3421 atggcgcaca aagtggcggt aatgtacgcc ggccaaatcg tggagaccgc gcaagcccgg
     3481 gagttttttg ccaatcccgc gcatccttat tcgcgcaagc tgttcgcctc gcttccccat
     3541 tccaacaagc gcaccgggaa acttgaaatt atcaaaggca cggtgccgcc gctaacccgc
     3601 gattttcaag gctgccgttt tgccgagcgc tgcgattttg cctgggacaa atgccggaaa
     3661 attgatcccg gttggagcga agcggcctgt gaccatactg tgcgttgcca tctttattcc
     3721 gaagggaagg ctgcaaaagt tgaaggcagc gcgcctgctg cgggctacaa aataacgcaa
     3781 acgaatgaac ctgtcgccga cttactggat gtttcagggc tcaaggtgta ttttccggtg
     3841 cacaaaggtt tattcaagcg cgtggtcggg tatgtcaagg cggtggatgg cgtcacgctg
     3901 gaaatcaaaa aaggccgcac cctcgctttg gtgggggaat cgggatgcgg caagaccacg
     3961 gtaggcaagg gcatcctgca attagtgccg gtgacttcgg gcagcgtgcg ttacaacgga
     4021 atcgacctca cccgactttc gcgcagcgat ttgcgttcaa gacgttccga gtttcagatc
     4081 atcttccagg acccgtattc ctcgctcaat ccgcgcatgc gggtttccga aatcatcgag
     4141 gaagggatga cggcgttggg aatcgccgcc agcgccgaag aacgcgcaaa acgcggggat
     4201 gttttgctgg agcaggtggg cttgtcgccg gaaatgaaga gccgctaccc gcatgaattt
     4261 tccggcgggc agcgccagcg catcgcgata gcccgcgcgc tggcggtgaa tccgaagctg
     4321 attatttgcg acgaaccgac cagcgccctg gatgtctcgg tgcaggcgca gattctgaat
     4381 ttgctgaaag agctgcaaga ccggctggga ctcgcttacc ttttcatcac ccataacatc
     4441 tcggtggtgg aatacctcgc gcacgaagtg gcggtgatgt atctgggaag gattgtggaa
     4501 accggcacgg tggaggaagt attaaacaac ccgaaacatc cgtacacccg cgcattgctg
     4561 tcggcggtgc cggtgattga taagagcggc aaaagggaaa ttatccgcct aaaaggcgat
     4621 ctgccttcgc ccttgaatcc gccgtccgga tgtcacttcc atccgcgctg ccccgacgtc
     4681 atgcctgtat gcaaggagag ttatccggga gacagccgtt tcagcagcac gcattgcgcg
     4741 cgctgtcatc tctacccaac ggggaactga ccggcattgc acgattgctt cgcgttggct
     4801 tgcaatgact ggtggggcta gaaccggttc ttccactcgc gcaccgctgc aaataccgct
     4861 accgggtcgt tgagcgactt gcccgccgct ctggttgcgg ttttgatgat ttgcggatgg
     4921 ttgcagcgca ggaaaggatt ggtggctttt tccagccgga tgtcggaggg cacggtgggc
     4981 aagccttgct cgcgcagagc acacgcccgg gtttcgcgtt caatcagcgc ggggttatcg
     5041 ggatcggcgg ctttggcgaa gcggatgtta gcaagcgtgt attcgtggcc gcagtaaacc
     5101 agggtttcat ccggtaaagc gacgagcttt tgaagggacg tgtacatttg ccgcgcggtg
     5161 ccttcgaaca atttgccgca gccgcatgca aacagcgtgt cgccacaaaa cagcaggttt
     5221 gcgccataat aggcgatgtg gccggcggtg tgtcccggaa tatcgagcac ggtgaattcc
     5281 aggcccagct gcggcaccgt cacccggtcg ccttcgacga gccgatgggt gagggtggaa
     5341 atggattcat ggcgcgggcc gtagaccgga acccggaaac gctggagcag gccgctgttg
     5401 ccgccgacat ggtcggcatg atgatgcgtg cagagaatcg agaccagctc gagcttttcg
     5461 cgctccaggt aagcgagcac gggcgtcgcg tcgccggcat caacgacggc ggcacagtgt
     5521 tggttgcgga tgacccagat ataattatcc cggaacgccg ggacggggac gacagttata
     5581 ttgctcatga cttatcttta cggttaatgc gcgcaaatgt caacgacact ttcggactgg
     5641 tttgaaacgc ccctcgggca atacctgctc gagcaggagc agacttattt cgacaaaacg
     5701 gtggcggatg tgttcggctt caacgccgtg caactagggc tggcgcacca cgattttctg
     5761 cgcatgagcc gcattccgct gcgcgtaagt gcgggaccgg acatcgaagc cagggtgaag
     5821 ctcgatttcc cgctgctgcc gtttgccacc aacagcattg atttgctgat gttgccgcac
     5881 gtgctggaat tcagcgataa cccgcatcag attttgcgcg aagcggagcg tgtgctgctg
     5941 cccgaggggc agttgattat ctgcggcttc aatccgcgca gcctgtgggg tctgtcgcga
     6001 cagtttggac aaagcagcgg cgagtacccg tggtgcgggc gcttcatttc gctgccgcgg
     6061 gtcaaggatt ggctggcgct gctcggcttt gaaatgacgg cggggcggct gggctgttat
     6121 gtgccgccat tcaggcagga aaaatggctg cggcgctttc gttttctcga agcggcgggc
     6181 gaccgctggt gggcgattgc cggcggcgtt tatttcctgc aggccatcaa gcgggtgcag
     6241 ggcctgcggc tgatcaagcc caactggaac gaagcattga gaccaaagaa aagcctggct
     6301 ccggtgcgcc gcgaatccaa ggccaaggta ctgcagttca agacgcggcg cccgagtcct
     6361 tccatctgat ggctgaaacc gtcgaaatct acaccgacgg cgcgtgcaag ggaaatcccg
     6421 gtgttggggg ttggggcgcg ctgctcaaat actccggcca tgtcagggaa ctcttcgggg
     6481 gcgaagcgca gaccaccaat aatcgcatgg aattaaccgc ggtcatcgaa gcgctttccg
     6541 cgctcaaacg tccttgcaat gtgaaactgc acaccgactc gcgctatgtg cagcagggca
     6601 tcagcgaatg gatacacgac tggaaaaaac gcaactggcg cagcgccgac aagaaaccgt
     6661 taaagaatag cgatttgtgg aaacgtctgg accaacttgc cgcaggacac cagatcgaat
     6721 ggatttgggt gcagggccac agcggcgacg aaggcaatga gcatgccgac agccttgcca
     6781 atcgtggggt cgaatcgatg caattaaagc gtgaggagta aggagtgacg cggcaaatca
     6841 cactcgatac cgaaaccacc ggcctggagc ccaacctcgg gcaccgcgtg gttgagctgg
     6901 ccgcggtgga attgctgaac cgcaggctca ccgggaaccg tttccacagc tatctcaatc
     6961 ccgaacgcga cagcgacgcc gctgcgctgc agattcacgg cctaacccgc gaatttttgc
     7021 aggacaagcg caggttcaag gatattgtca acgaattcct cgagtacatc agcggcgccg
     7081 aactgattat ccacaacgcg gcttttgacg aggcgtttct cgattacgag ctgggactgt
     7141 tgggcttcaa ggggcttaag gaatactgcg ccggcatcgt ggatacgctg aagatggcga
     7201 aggagctgca tccgggcaag aaaaacagcc tcgatgcctt gtgcgagcgc tatgaggtgg
     7261 acaattccaa gcgcacgctg cacggcgcgc tgctcgatgc ggagctgttg gccgacgtgt
     7321 atgtagcgat gacgcgcggc caggaaagcc tgatgatgga tgaagaggat ggcgcattac
     7381 cacgtgccga agttgcgcac gccatcaagc gcgatttgtt gataatcacc gcttcgccgg
     7441 aggagctgca ggcccacgcg gagcagttgc aggaaatcga caaggcgagc ggcggcgact
     7501 gcgtgtggaa taaaatcgcg caagcccggt aacgcaagcg gcgcaacggg cgtcatcata
     7561 cgggaggtgc caaatgagca agattctcaa tgaagtactc gcggcaaaca aggaatactc
     7621 caaaaatttc ggggacaagg gaaaactccc gataccgccg gcgcggcatt tcgccgttct
     7681 cacctgcatg gacgcgcgcc tgcagccctc caggttcgcg gggctggcgg agggcgacgc
     7741 ccatgtgatc cgcaacgccg gcgggcgcgc gagcgacgac gcgatacgct cgctggtgat
     7801 ttcgtacaag ctgctcggca cccgcgaatg gttcgtgatt caccacaccg attgcggcat
     7861 ggaaaccttt accgacgaag tgatgcgatg gctgttggca aacagcctga aaaccgcttc
     7921 gcttacgaaa gacggctggc gtgatagcgg caaggggccg ggttcgatgg aagggcagtt
     7981 catagactgg cttaccataa aggatcagcc gaagagcatg tgcgccgatg tggcacgcat
     8041 caggaatcat ccactggtgc cgcgcgacat tcccatttac gggt
//
LOCUS       DATIOW010000018         8334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1733502, whole genome shotgun sequence.
ACCESSION   DATIOW010000018 DATIOW010000000
VERSION     DATIOW010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733502"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VLL03_00430"
     CDS             complement(<1..302)
                     /locus_tag="VLL03_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HSS45870.1"
                     /translation="MIQRKAASVVTRLHAKAKLFVLDTNVLMHDPTSLFRFQEHDIYL
                     PMATLEELDNNKKGMSEVSRNARQASRFLDEIVSDASINMDEGISLETHGGKDATGR"
     gene            complement(324..557)
                     /locus_tag="VLL03_00435"
     CDS             complement(324..557)
                     /locus_tag="VLL03_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS45871.1"
                     /translation="MPLEFFGNRVINCKTLMLNQPSPDFELPATGNKLFRLSALKGKP
                     LVIDRKGLLQRERRAAKVPGHAQEVLEYVQTLK"
     gene            complement(547..1350)
                     /locus_tag="VLL03_00440"
     CDS             complement(547..1350)
                     /locus_tag="VLL03_00440"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HSS45872.1"
                     /translation="MKLALKVDVRTMRGTRTGVPRLMELLQKHNAQASFFFNLGPDRS
                     LFPGAEVGRRYAETLRQTRAAGFEVGIHAYNSKLWKSKAARADAAWTLNQMQLACERF
                     DEIFKDAPRIHGAPGWQMSLHAFRLTQRLGFDYGSDTRGTCPFVPIYRGEIIACPQLP
                     TTLPTLDEAIASAGMEGAAGHLLKLSENPPASGHLYTLRAEREGRKLAPLFERLIESW
                     RAQGYELTSTHMLFETLETKNLPRHEVTLGTLRGLRGPLMLQAKEFLAA"
     gene            complement(1347..2420)
                     /gene="mnmH"
                     /locus_tag="VLL03_00445"
     CDS             complement(1347..2420)
                     /gene="mnmH"
                     /locus_tag="VLL03_00445"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008752.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HSS45873.1"
                     /translation="MHPYPLRDVVNVAQLRDFDELIDVRSPQEFNEDHIPGAINCPVL
                     NDGERARIGSLYKQVSSFEAKKTGAVLIARNIARHIETLFLGKDQGWRPLVYCWRGGK
                     RSEAMLHVLKQIGWNAARLEGGYKSYRAAVIAGLEELPQRFGFRVICGPTGSGKSRLL
                     QALEELGAPVLDLEKLASHRGSVLGALPDAPQPRQKMFESLIWRQLQRFDPKRPVYVE
                     AESKKIGNVRVPDRLLEAMWNSPCIRLEADAALRATLLKKEYQHFLEDPVALHQKLGF
                     LTARYGHEVIRRWKGYSANRQWDELVLELLEQHYDPAYGKSTVKHYTLYQDALAVRVN
                     GPDDAEMRLLAQTLRSEENPKTK"
     gene            2426..3484
                     /gene="selD"
                     /locus_tag="VLL03_00450"
     CDS             2426..3484
                     /gene="selD"
                     /locus_tag="VLL03_00450"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HSS45874.1"
                     /translation="MNQIIDLGRIKLTQFSHGGGCGCKIAPAVLSEILSQSAIGKTWP
                     NLLVGTESSDDAAIYRLNHNQALIATTDFFMPIVDDPYDFGRIAAANALSDIYAMGGQ
                     PILALAIVGMPIDKLPVAVIQRILAGGEAVCTQAGIPIAGGHSIDSPEPIYGLVGLGL
                     VTPDKLKRNDTARAGGALILGKALGIGILSAALRKGKLSVEGYRQMLESATQLNTAGV
                     GLAEMPGVHALTDVTGFGLLGHLLEMCRGAGLGAEIDFAKLPVLPEALELAKQDFGPG
                     AIGRNWAGYGKEVILPEAMPKWQKNLLCDPQTSGGLLVACSEEAVEKVLELFHQQGFQ
                     YAAKIGRMTQGAAKVTVR"
     gene            complement(3496..3663)
                     /locus_tag="VLL03_00455"
     CDS             complement(3496..3663)
                     /locus_tag="VLL03_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS45875.1"
                     /translation="MGENKSKLPIYEVEFIPLERRLASRRKQNQPVPVEQRKGSSRRG
                     PEGASHSPKEK"
     gene            complement(3746..4282)
                     /locus_tag="VLL03_00460"
     CDS             complement(3746..4282)
                     /locus_tag="VLL03_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HSS45876.1"
                     /translation="MVFGDSLSSGYGLPQDAGWVSLLKQRLQQEHPEYQVINASITGE
                     TTRGGLSRIENSLKTHQPSIVILELGGNDGLRGQRIEIARDNLEAIIKACKQRRAEVL
                     LIGMRLPPNYGPAYTQKFQSIYPELAKRQRVALVPFMLEGFSDNPALFQPDGIHPAAA
                     AQPLILDRVWKRLRPLLR"
     gene            4362..5030
                     /locus_tag="VLL03_00465"
     CDS             4362..5030
                     /locus_tag="VLL03_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSS45877.1"
                     /translation="MEPIVQAIALTKQVRNGATDLTILDKVSFHVAAGEALAILGASG
                     SGKSTLLGLLAGLDTPSSGTVKLNGVDLFALDEDGRAAVRAGSIGFVFQSFQLLPALT
                     ALENVMLPLELAEMEDARAQALRLLDRVGLGQRLHHYPKQLSGGEQQRVALARAFVGR
                     PKLLLADEPTGNLDSVTGEQIIDLLFELNREQGATLLLVTHDEALSKRCGRLLYLAAG
                     RLAP"
     gene            5027..7675
                     /locus_tag="VLL03_00470"
     CDS             5027..7675
                     /locus_tag="VLL03_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSS45878.1"
                     /translation="MNLLPLSLRMLARDWRSGELRVLAAAMVIAVGSVTAVAFFTDRV
                     GQALDKEANQLLGADLVLASDRGWREDFADEARRRGLQVTHALKFPSMVQNAESGQLT
                     EIKAVAEGYPLRGELRIATETDKPGSTVNAAPKPGTVWADQRLLTRLGLKLGDSVELG
                     TARFKVTAVVTEEPDYAVGFFNIAPRLIMNQDDVSATGLVQTGSRVNYYWLFAGEPER
                     ISAFRQWSEPRLASGERLQDVKDARPEIRAALERAQKYLGLAALMSVVLAAAAVALSA
                     RRFVERHLDGCAMMRCLGSSQGSMLRLYFYHFLVLGLAASLAGCLLGLIAQQLLAHWL
                     GVLVAKNLPPPSPLPLLQGLVSGLALLLGFALPPLIRLSQVPTLRVMRRELGLPKGLG
                     GAGYVLGLMLIAGLLLWQANELKLGLYVLGGLVLSAAVSVLAGFGLIKALAFIPQGVG
                     VTWRYGLASARRHSTASVIQVVCLSAGMTALLLLTLVRGDLLQSWRASLPADAPNRFL
                     VNIQPDQLLSLKEFFASHRFDALRFFPMVRGRLTAINNQRVSSSDYPDERAKRLIERE
                     FNLSWAARMQEDNQIVEGAWWAEKEHGQSQFSVEQGLAETLGIRLGDTLAYDIAGATI
                     RGRVTSLRKVDWDSFRVNFFVIAPPGMLESYPVSYITSFHLSPQRALVINDLVKAFPN
                     FLVIDVEAILAQVQKIMDQVVQAVEFVFLFGLFAGLMVLYAAIVATQDERLLEAGMLR
                     TLAPAAGRFCAPSWPSSSPSAPWRACSRQRPPARWVWYLPRRSCIFLMRLIPGSGCWV
                     LLAAGWACLWRAGRNCALFCYGPHCKPCAASVNAEAAARLALIGYTGVNSRSVYERNS
                     AWRGCGINLGYPHLDGCPLRRPDSAQ"
     gene            7581..>8334
                     /locus_tag="VLL03_00475"
     CDS             7581..>8334
                     /locus_tag="VLL03_00475"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HSS45879.1"
                     /translation="MSEILLGVVAVLILVILIWMGVRFAALIRLSNNLSAEVAQLMED
                     KHRSMLKDLHDGLTRQGDRLDEKLAQTSERLRATVDERLEQISGKVGERLDEGFKKTN
                     ETFVNVMARLATIDEAQKKIDGLTSNVVSLQELLGDKRSRGAFGEVQLEALVRNILPP
                     SAYAMQHTLSNGSRADCILRLPEPTGPVAVDSKFPLENYHRMFAAGVNEIERAQAQRQ
                     FKADVKKHVDDISNKYIILNETSDGAVMFVPAE"
BASE COUNT         1525 a         2278 c         2653 g         1878 t
ORIGIN      
        1 cggccggtcg cgtccttgcc gccgtgcgtt tcgagtgaaa tgccctcgtc catgttgatg
       61 gaggcgtcgc tcacgatttc atcgaggaag cggctcgcct ggcgtgcgtt gcgcgaaacc
      121 tcggacatgc ccttcttgtt attgtcgagc tcctcgaggg tagccatggg gaggtaaata
      181 tcgtgttcct ggaagcggaa cagactggtc ggatcgtgca tcaggacgtt ggtgtcgagc
      241 acgaacagct tggctttggc gtgcaggcgg gtgacaacag aagcggcttt tctctggatc
      301 atgcagtttt ttccgttaga tgattatttc agggtttgca catactccag gacttcctgc
      361 gcatggccgg gcactttcgc tgcgcgcctt tcgcgttgca gcaggccctt gcgatcgatg
      421 acgagtggct tgcccttgag agcagagagg cgaaaaagct tgttcccggt tgcagggagc
      481 tcaaaatcag gagaaggttg attgagcatt aacgttttgc agtttataac acgattgccg
      541 aaaaactcaa gcggcaagaa attccttggc ttgcagcatg agcggtccgc gtaaaccgcg
      601 caaggtgcca agggtcactt catgccgcgg caggttcttg gtttccagcg tttcaaacag
      661 catatgcgtt gaggtgagct cgtagccttg agcgcgccaa ctttcgatga ggcgctcgaa
      721 cagcggtgcg agttttctgc cttcgcgctc ggcgcgcaag gtgtaaagat ggccactggc
      781 aggtggattc tcgctgagct tcaggagatg tccggccgcg ccttccatgc cggcgctggc
      841 aatcgcttca tcgagcgttg gcagggtggt gggaagctgc ggacaggcga tgatttcacc
      901 gcggtaaata gggacgaaag ggcaggtgcc gcgggtatcg ctcccgtaat caaagcccag
      961 gcgctgggtg agccggaaag cgtgtaagct catctgccag cccggcgcac cgtggatgcg
     1021 gggagcgtcc ttgaaaatct cgtcgaaacg ctcgcaggcg agttgcattt gattcaatgt
     1081 ccatgccgca tcggcccggg cggctttgct tttccacagc ttgctgttgt aggcatgaat
     1141 gcctacttca aagccggcag cgcgcgtttg gcgcagtgtc tcggcgtatc gcctgccgac
     1201 ttccgcgcct ggaaagaggc tgcgatcggg gcccaggttg aagaaaaagc tggcctgcgc
     1261 gttgtgcttt tgcagcagct ccatgaggcg cggcacgccg gtgcgggtgc cgcgcatggt
     1321 tctcacgtcc accttgagcg cgagtttcat tttgttttcg gattttcttc ggaccgaagg
     1381 gtttgcgcaa gcagacgcat ttccgcatca tccgggccat tcacgcgcac cgccaacgcg
     1441 tcctggtaaa gtgtgtaatg cttgaccgtg gatttgccat aggcggggtc gtagtgctgc
     1501 tcgagcaatt ccagcaccag ttcgtcccat tggcggttgg cggagtatcc tttccagcgc
     1561 cggatgactt catggccata gcgcgcggtg aggaatccca gcttctggtg gagcgcgacg
     1621 gggtcttcca gaaagtgctg atactctttt ttcaataaag ttgcgcgcaa cgcggcatcg
     1681 gcctcgagcc ggatgcaggg gctgttccac atggcttcaa gcagccggtc cggcacgcgc
     1741 acgttgccga ttttcttgct ctccgcctcg acgtacaccg ggcgttttgg gtcgaagcgc
     1801 tgcagttgcc gccagatcag gctttcgaac attttttgcc tgggttgcgg agcgtcgggc
     1861 agtgcaccca gcaccgagcc gcgatgcgag gcgagttttt ccaaatccag aactggtgcg
     1921 ccgagttcct caagcgcctg caacaagcgg cttttgccgg agccggtcgg gccgcagatg
     1981 acccgaaaac cgaaacgttg gggcagttct tccagcccgg caatcaccgc tgcccgataa
     2041 gatttgtagc cgccttctag ccgcgcggca ttccagccga tttgcttgag cacatgcagc
     2101 atggcttcac tgcgcttgcc gccgcgccag cagtaaacta gcggccgcca gccttggtcc
     2161 ttgccgagga acagcgtttc aatatggcgg gcgatgttgc gtgcgattag cacggcaccg
     2221 gtttttttcg cttcgaagct ggatacttgc ttgtaaaggg agccgatacg cgctctttcc
     2281 ccgtcgttga gcaccggaca attgatggcg ccgggaatat ggtcttcgtt gaactcttgg
     2341 ggcgagcgca cgtctatcaa ttcgtcaaag tcgcgcaact gtgctacgtt aaccacgtca
     2401 cgaagcgggt aaggatgcat tttcaatgaa ccagataatt gacttgggtc ggataaagct
     2461 gactcaattc tctcacggcg gcggctgtgg atgcaaaatc gcgccggcgg tgttgagcga
     2521 aatcctgtca cagtcggcaa tcggcaagac ctggccgaat ttgctggttg gcaccgaatc
     2581 gagcgacgac gcggccattt atcggttgaa tcataatcag gcgctgatcg ccaccaccga
     2641 tttcttcatg cccatcgtcg atgaccccta cgactttggc cgcatcgccg cggccaatgc
     2701 gctgtcagac atttatgcga tgggaggcca gccgatcctg gcgcttgcca tcgtcggcat
     2761 gccgatagac aaactcccag tggccgtcat tcagcgcatt ctcgccggcg gcgaagcggt
     2821 gtgcacccag gccggcattc ccatcgccgg agggcactcg atcgattcgc ccgaaccgat
     2881 ttacgggctg gtcggtttgg gtctggtgac tcccgataaa ctgaagcgca acgacaccgc
     2941 gcgcgcgggc ggcgccttga tactcggcaa ggcgctgggc atcggcatcc tcagcgccgc
     3001 gctgagaaaa ggaaaacttt ccgtggaggg ttaccggcaa atgctggaga gcgcgaccca
     3061 gctcaatacc gcaggtgtgg ggctggcgga aatgccggga gtacacgcgc ttaccgatgt
     3121 tactggtttt ggtttgctcg ggcatttgct ggaaatgtgc cgcggcgccg ggcttggtgc
     3181 cgaaattgat tttgctaagt tgccggtatt gccggaggca ttggaactcg ccaaacagga
     3241 tttcggtccg ggcgcgattg gccgcaattg ggctgggtat ggaaaagaag tgattttgcc
     3301 tgaagcgatg ccgaaatggc agaagaacct gctatgcgat ccgcaaacca gcggcggcct
     3361 gcttgtcgct tgcagcgagg aagctgtgga gaaggtgctg gagctgttcc accaacaggg
     3421 gtttcagtac gccgccaaaa tcggccgcat gacccagggt gcggccaaag tcacggtgcg
     3481 ctgaggcgcg ccggcttatt tctccttggg ggaatggctt gcgccttcag gccctctgcg
     3541 cgaagacccc ttgcgctgct ccaccggcac gggttggttc tgcttgcgcc ggcttgccag
     3601 ccttctttcc agaggaataa actcgacttc gtagatcggc agcttggatt tgttctctcc
     3661 catgcgtttt tgctttcgct gtgttgagcc tgcgataggg ttaggttgtc tgtggggatg
     3721 aatatagcat attttgtccg gtctttcagc gcagcagcgg gcgcagacgt ttccacactc
     3781 tgtcgaggat aagcggttgc gcggcggccg ccggatggat gccgtcaggc tggaacaggg
     3841 cgggattatc ggaaaaacct tccagcatga acggcaccag cgccacccgc tggcgtttgg
     3901 cgagttcggg ataaatgctc tgaaactttt gggtgtaagc gggtccgtaa tttggcggca
     3961 gacgcatgcc aatgagcagc acctcggcgc ggcgctgctt gcaggctttg atgatggcct
     4021 ccagattatc gcgcgcgatt tcgatgcgct gtccgcgcag tccgtcgttg ccgcccagtt
     4081 caagtatgac gatggacggc tgatgggttt tcaggctgtt ctcgatgcgg cttaaaccgc
     4141 cgcgcgtggt ttcgccggtg atgctggcat taatgacttg gtattccggg tgctcttgtt
     4201 gcaagcgttg tttaagcaga ctcacccagc cggcgtcttg cggcaggcca taacccgaag
     4261 acaggctgtc gccaaatacc atgagagttg cgccagtggc gtgaagcggc agccctaacc
     4321 agaaaacagc aaggacgcgg gcgacgatga cggaaaaacg catggaaccg attgtgcagg
     4381 caatcgcact taccaagcaa gtgcgcaatg gcgcaaccga tttgaccata ctggataaag
     4441 tgagtttcca tgtcgcggcg ggcgaagcgc tggcgatact cggcgcctcg ggttcgggga
     4501 aatccacttt gctgggattg ctcgcggggc tggatacgcc gagttccggc accgtgaagt
     4561 tgaacggcgt agatttattt gcgctcgatg aagacgggcg tgccgccgtg cgcgccggat
     4621 ccatcggttt cgtgtttcag tcttttcagt tgttgccggc gcttaccgcg ctggagaatg
     4681 tgatgttgcc gctggagctg gcggagatgg aagatgcgcg cgcgcaggcg ttgcgcttgc
     4741 tcgatcgcgt ggggctgggg cagcggctgc accattaccc caagcagctt tccggcggcg
     4801 agcaacagcg cgtggcgctg gcccgcgctt ttgtcggcag gcccaaattg ctgctggcgg
     4861 acgagccgac cggaaatctc gacagcgtga ccggcgagca aatcatcgat ctcctgtttg
     4921 aattgaaccg ggagcagggc gcgaccttgc tgctggtcac tcacgatgaa gctttgtcga
     4981 agcgctgcgg ccgcttgctg tatcttgcgg cggggcggct tgcgccgtga atttactccc
     5041 gctctcgctg cgcatgctgg cgcgcgactg gcgctcgggc gaattgcgcg tgctggccgc
     5101 ggccatggtg attgcggtgg gcagcgttac cgccgtcgct tttttcaccg accgtgtcgg
     5161 ccaggcgctg gataaggaag ccaaccagct gctcggcgcg gatttggtgc tcgcttccga
     5221 tcgcggctgg cgcgaggact tcgccgacga agcgcgccgc cgcggcctgc aagtcacaca
     5281 cgcgcttaag tttcccagca tggtgcaaaa cgcggagagc gggcagctta ccgaaatcaa
     5341 ggcagtcgcg gaaggctatc ccttgcgcgg cgaattgcgc atcgccaccg aaaccgataa
     5401 accgggcagt acggttaacg ctgctccaaa accagggacg gtctgggccg accagcgtct
     5461 gttaactcgc ctcggcctca agctggggga tagcgtggag ctcggcactg cgcgatttaa
     5521 ggtgacggct gtggtgaccg aagagccgga ttatgcggtg ggatttttca acattgcccc
     5581 gcggctcatc atgaatcagg atgatgtatc cgccaccggc ctggtccaga ccggcagccg
     5641 tgtcaattat tattggcttt ttgccggcga gccggagcgg atttccgctt ttcgccaatg
     5701 gtcggagccg cggctggcat cgggcgagcg cctgcaggat gtgaaggatg cgcgcccgga
     5761 aatccgcgcc gcgctggaac gcgcgcaaaa atatcttggg ctggcggcgt tgatgagcgt
     5821 ggtgctggcg gcggcggcgg tggcgctttc ggcgcgccgc tttgtcgagc gccatctcga
     5881 tggctgcgcc atgatgcgct gcctgggttc cagtcaaggt tcgatgctgc ggctttattt
     5941 ttatcatttt ctggtgttag ggctggcggc gagcttggca ggttgcctgc ttggattgat
     6001 tgcacaacaa ttgctcgccc attggttggg cgttttggtt gcaaagaacc tgcctccgcc
     6061 cagcccattg ccgttgctgc agggtttggt cagcggcttg gctctgttgc tgggttttgc
     6121 gctgccgccg ctcatacgct taagccaggt gcctaccttg cgcgtaatgc ggcgcgaact
     6181 gggcctgcct aaaggcttag gcggcgccgg ttatgtcttg ggcttgatgt tgattgccgg
     6241 cttgctgttg tggcaggcga atgagctgaa actgggcttg tatgtgctgg gaggcttggt
     6301 gttgagtgct gcggtttcag tgcttgcggg atttggatta atcaaggcct tggcatttat
     6361 tccgcaaggt gtgggcgtga cctggcgtta tggcttggcg agcgcaagac gccactccac
     6421 ggccagcgtc atccaagtgg tttgcttaag cgcgggaatg acggctctgt tgttgctgac
     6481 gctggtgcgc ggcgatctgc tgcaaagctg gcgagcaagt ttgcccgcgg atgcgcctaa
     6541 ccgcttttta gtcaacatcc aaccggatca attgctatcg ctcaaggagt tttttgcttc
     6601 gcatcgtttc gatgcgctgc ggtttttccc catggtgcgt ggcaggctga ccgccatcaa
     6661 caaccagcgc gtatcttctt cggattatcc ggatgaacgc gccaagcgcc tgatcgaacg
     6721 cgaattcaat ctgtcctggg cggcgcggat gcaggaagac aatcaaatcg tggagggcgc
     6781 gtggtgggcc gagaaagagc acggccaatc gcagttttcg gtggagcagg gtttggcgga
     6841 aacactgggt atccgtctgg gcgatacgct ggcgtacgat attgccggcg ctacaatccg
     6901 gggccgggtc accagcttgc gcaaagtgga ctgggacagc ttccgggtga attttttcgt
     6961 gatcgcgcct cccggcatgc tggagagtta tcccgtcagc tatatcacca gctttcattt
     7021 gtccccgcag cgtgcgctgg tcattaacga tttggtcaag gctttcccga attttctggt
     7081 tatcgatgtt gaagcgattc tggcgcaggt gcagaaaatc atggatcagg tggtacaggc
     7141 ggtggaattc gttttcctgt ttggcttgtt cgcggggttg atggtgctct atgccgccat
     7201 cgtcgcgacc caggacgagc gcttgctgga agccggcatg ctgcgcaccc tggcgccagc
     7261 cgcgggcagg ttttgcgcgc ccagctggcc gagttcgtcg ccgtcggcgc cttggcgggc
     7321 ttgctcgcgg cagcggcctc cggcgcgctg ggtttggtac ttgccgagaa gatcttgcat
     7381 attccttatg cgtttaatcc ctggctctgg ctgctgggtg ttgttggcgg cgggttgggc
     7441 gtgcctttgg cgagctggcc gcaactgcgc gctattttgt tacggccccc attgcaaacc
     7501 ttgcgcagct tcggttaatg ccgaagcggc ggcgaggctg gcgctgatag gctatactgg
     7561 cgtcaactcg cggagtgttt atgagcgaaa ttctgcttgg cgtggttgcg gtattaatct
     7621 tggttatcct catctggatg ggtgtccgct tcgccgccct gattcggctc agtaataacc
     7681 tgtcggccga agtggcgcag ctgatggaag acaagcaccg ctccatgctc aaggatttgc
     7741 atgacggcct gacgcgccag ggcgaccggc tcgatgaaaa actggcgcaa acctcggaac
     7801 gcctgcgcgc cacggtggat gagcgccttg agcaaatcag cggcaaagtc ggcgagcgtc
     7861 tcgacgaagg tttcaagaaa accaatgaaa cctttgtcaa tgtgatggcg cggcttgcca
     7921 ccatagacga agcgcagaag aaaatcgacg ggctcaccag caatgtggtc agcctgcagg
     7981 agcttttggg tgacaagcgt tcgcgcggcg cctttggcga agtgcagctc gaagccttgg
     8041 tgcgcaacat attaccgcct tccgcgtatg cgatgcagca caccctttcc aatggcagcc
     8101 gcgccgattg catcctgcgc ctacccgagc ccaccggccc ggtggcggtg gattccaagt
     8161 tcccgcttga aaactaccat cgcatgttcg ccgcgggtgt caacgaaatc gagcgcgccc
     8221 aggcgcagcg gcaattcaag gccgacgtga aaaaacacgt cgacgacatc agcaataaat
     8281 acatcattct caatgaaacg tccgatggcg cggtgatgtt tgtcccggcg gaag
//
LOCUS       DATIOW010000019         2298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_108297, whole genome shotgun sequence.
ACCESSION   DATIOW010000019 DATIOW010000000
VERSION     DATIOW010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108297"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VLL03_00480"
     CDS             complement(<1..439)
                     /locus_tag="VLL03_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS45880.1"
                     /translation="MPRENLLKFLKKTWLVLFLVAGFAFVFDYIREQTGKLEGVLYFS
                     ALYFFLALTVHSAFWTGAAWCWGRVVLFSNGTRLPLLVCFSHLALLTIGKYFPGKIWG
                     MAARAALMKEQDINLSNALLATFHEQFIFLHAAVVLSGIFFGVL"
     gene            complement(454..588)
                     /locus_tag="VLL03_00485"
     CDS             complement(454..588)
                     /locus_tag="VLL03_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS45881.1"
                     /translation="MTVTAKITSIGDSFAFAATTGIFFGYHPARQAAYLNPAEALRYE
                     "
     gene            complement(659..979)
                     /gene="soxZ"
                     /locus_tag="VLL03_00490"
     CDS             complement(659..979)
                     /gene="soxZ"
                     /locus_tag="VLL03_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HSS45882.1"
                     /translation="MADGIKIRAQLREDVADIKVLISHPMETGQRKDSQGNLIPLHFI
                     KKVSATLNGKLVLDAQWSQGIAKNPFLGFKIRGARAGDKVAVSWSDNLGEANFIETTV
                     TPAN"
     gene            complement(984..1445)
                     /gene="soxY"
                     /locus_tag="VLL03_00495"
     CDS             complement(984..1445)
                     /gene="soxY"
                     /locus_tag="VLL03_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HSS45883.1"
                     /translation="MHRLRRAFLQNAGVFFIAVLAGAIRPVRLFAAEWNQSGFEAKTL
                     AEALKSIGATGAAVSSDILIKAPDLAENGARVQIEVLSNIPGTQTITLVAEKNPFPLV
                     ATFEFDNGAEAFVSTYIKMGESANVRAVVNAGGKIYTAAKEVKVTIGGCGG"
     gene            complement(1557..2075)
                     /gene="moaC"
                     /locus_tag="VLL03_00500"
     CDS             complement(1557..2075)
                     /gene="moaC"
                     /locus_tag="VLL03_00500"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HSS45884.1"
                     /translation="MAGKLTHFDAKGQAFMVDVAAKGETRRIARATGRIVMQPATLKK
                     ILEGRAKKGDVLGVARIAAIQAAKRTAELVPLCHPLALTRVSVEFSVDQKASAVDCLA
                     TAETVGRTGVEMEALTAVNVGLLTIYDMCKAVDRGMHMENIRLLHKEGGKSGLWRARD
                     AKPAKRRKAQKA"
BASE COUNT          475 a          671 c          637 g          515 t
ORIGIN      
        1 agagtacccc aaaaaaaatc ccgctcagta caacggcggc atgaagaaag atgaattgtt
       61 catgaaaggt ggcaagcagg gcgttgctca aattaatatc ttgctctttc atcagggctg
      121 cacgggcggc catgccccat attttcccgg ggaagtattt gcctatcgtc aacaaggcta
      181 aatgggaaaa gcacacgagt agcggcagtc tggttccatt gctgaacagg accacccggc
      241 cccagcacca cgcggcgcca gtccagaaag cagaatgcac cgtcaaggcc aaaaaaaagt
      301 aaagagcgga aaaataaagc actccttcca atttcccggt ctgctcccgg atgtaatcga
      361 atacgaacgc aaagccagcc acgagaaaaa gtaccagcca tgttttttta aggaacttta
      421 aaagattttc ccgcggcatc ggtggaccag cctttattca tagcgcaaag cctcggcagg
      481 atttaaatag gctgcctggc gcgccgggtg gtagccgaag aatataccgg tggtggcggc
      541 gaaagcaaat gaatcgccga ttgaagtaat cttggctgtc accgtcatat gccgcggcgg
      601 cgcaaacaat ggcgaaattc aggggttaaa gcgcgcgaaa tgtccagccg agggcggttc
      661 agtttgccgg agtgacggtg gtctcgatga aattggcttc gcctaggttg tcgctccagc
      721 ttaccgccac tttatcgccg gctctggcgc cgcggatttt gaagccgagg aaggggttct
      781 tggctattcc ctggctccac tgcgcgtcca gcaccagttt gccgttcagc gtggcgctta
      841 ccttcttgat gaagtgaagc ggtatcaggt taccctgact atccttgcgc tgaccggttt
      901 ccatggggtg actgatcagc accttgatgt cggcgacatc ctctcgcagt tgcgcccgga
      961 tttttatccc atcagccatt ttttcatccc ccgcaaccgc ctatcgtgac cttgacctct
     1021 ttcgccgcgg tatatatttt gccgccggcg tttaccaccg cccgcacatt ggccgattcg
     1081 cccattttga tgtaggtgga aacaaaagct tccgcgccgt tgtcgaactc gaaggtcgcg
     1141 accaacggga aggggttttt ctccgcgaca agggtaatcg tctgggtgcc ggggatgttg
     1201 ctcaggactt cgatctgcac ccgagcgccg ttctcggcga gatccggggc tttgatcagg
     1261 atgtcgctgc tcactgccgc gccggtggcg cctatgcttt tcagtgcctc ggccagcgtt
     1321 tttgcttcga agcctgactg attccactcc gcggcaaaga gccgaacggg acgaatcgcg
     1381 ccggcaagaa cagcgatgaa aaatacccct gcgttttgca gaaacgcacg gcgcagccta
     1441 tgcatagact cgagcctcgg acaaaatcat cgtattgccg tttatggtat cgtaaattgc
     1501 actacgcttg gtgctgcgac tgctagcttg aaaccccgcg gccaggctca gcaaggctaa
     1561 gctttttggg ccttgcggcg tttggcgggc ttcgcgtccc gcgcccgcca caggccggac
     1621 ttgccgccct ctttgtgcag tagccggatg ttttccatgt gcatgccgcg atcgaccgcc
     1681 ttgcacatat cgtaaatcgt gagcagcccc acgttcaccg cggtgagcgc ttccatttcc
     1741 acgccggtgc gccccacggt ctcggcggtg gcgaggcaat ccactgcgct tgctttctgg
     1801 tccacgctga attccaccga taccctggtc agcgcaagag gatgacaaag aggaaccaat
     1861 tcggcggtgc gcttggccgc ctgaattgcg gcaattcgcg ccacgcccag cacatcgcct
     1921 tttttggcgc ggccctcgag aatttttttc agcgttgccg gctgcatgac aatcctgccg
     1981 gtggcgcggg caatgcggcg ggtctcgccc ttcgccgcga cgtccaccat gaaagcctgc
     2041 cctttggcgt cgaaatgggt gagcttgccg gccatgcttt aggttattgt ggattaggaa
     2101 cttatttctg aaaggtttta tcatatcacc atgcgctcag cctacctaat cgcgctcctg
     2161 ctgctcgcca cgggcgttct cgccgaaggc ctgcccgagt tgggcgattc ctcgcaaacc
     2221 acgctttcgc cgcggcagga gcggcagctc ggcgagagca tcatgcgcga gatccgcgcc
     2281 gacccgagct atcttgac
//
LOCUS       DATIOW010000020         4375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_210030, whole genome shotgun sequence.
ACCESSION   DATIOW010000020 DATIOW010000000
VERSION     DATIOW010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210030"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VLL03_00505"
     CDS             <1..232
                     /locus_tag="VLL03_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="5'-methylthioadenosine
                     nucleosidase/S-adenosylhomocysteine nucleosidase"
                     /protein_id="HSS45885.1"
                     /translation="FVAVRVVVDTADMTFPRSAIRASDEYGRLHITKLLFSLLAHPAD
                     LSALIGLSKSFRAAQATLQTIARLAGPKLCLQ"
     gene            249..1241
                     /gene="hpnA"
                     /locus_tag="VLL03_00510"
     CDS             249..1241
                     /gene="hpnA"
                     /locus_tag="VLL03_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HSS45886.1"
                     /translation="MTSLVTGASGFVGSALARRLVAAGHRVKVLLRPTSDHRNIEELP
                     VEIAQGDLTDKASLERALEGCDSLFHAAADYRLWTRNPDALYQSNVSGTLNIMRVALE
                     TGVKRIVYTSSVATLGLNADGTPADENTPVTFADMIGHYKRSKFLAEAEVKKLVQEQG
                     LPAVIVNPSTPVGPRDIKPTPTGRMVLDAACGRTPAYVDTGLNLVHVDDVAAGHWLAY
                     ERGKIGERYILGARNMTLKEILTVVAKIAGHKPPSVRLPHNLVLPIAYLSEAWAWLTD
                     GSEPRATVEGVRLSKKYMFFSIEKAQRELGYHPRAVEQALQDAVDWFRANGYCG"
     gene            complement(1238..2467)
                     /gene="ispG"
                     /locus_tag="VLL03_00515"
                     /gene_synonym="gcpE"
     CDS             complement(1238..2467)
                     /gene="ispG"
                     /locus_tag="VLL03_00515"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HSS45887.1"
                     /translation="MSLQTISRRVSAGVRIGPVTIGGGAPIVVQSMTNTDTADVESTA
                     KQVAALAQAGSELVRITVNSAEAAAAVPAIRGHMEQMGCKAPLIGDFHFNGHKLLTEY
                     PGCAEALSKYRINPGNVGRGSKRDEQFATMIEVACKYGKPVRIGVNWGSLDPALLTRM
                     MDENAKLPEPKDANEVTREALIVSAIDNARRAEEIGLAHDKIVLSCKVSGVQDLIAVY
                     SELARRCDYPLHLGLTEAGMGSKGIVASTAALSVLLQQGIGDTIRISLTPEPGGDRTR
                     EVVVAQEILQTMGLRSFTPMVVACPGCGRTTSTFFQELADKIQTYLRNQMPAWREKYA
                     GVEKMNVAVMGCVVNGPGESKHANIGISLPGSGETPVAPVFVDGQKTVTLKGANIAEE
                     FQKIVDDYVRTRYAKRS"
     gene            complement(2654..3031)
                     /locus_tag="VLL03_00520"
     CDS             complement(2654..3031)
                     /locus_tag="VLL03_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS45888.1"
                     /translation="MNCGVLAFFGLSFGGLAIFFAQLDVAFALAGVLAFASVLGSLAV
                     GLALASVHAFTVHFAFCRNGAADTGSNKHAGRSNSQSNTCEFFNSSHGLSLLKLQKPV
                     FKSGRQAGLSIMRPGCALMNSED"
     gene            3186..>4375
                     /gene="hemA"
                     /locus_tag="VLL03_00525"
     CDS             3186..>4375
                     /gene="hemA"
                     /locus_tag="VLL03_00525"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HSS45889.1"
                     /translation="MQLFAFGINHKTAPLAMREQVAFHAQTLGVALRDLVNRRPVAEA
                     AIISTCNRTEVYCNTPEPEAAVEWLSDYHHLKTQNIQPYLYTLPQEKAVKHAFRVASG
                     LDSMVLGEPQILGQIKQAVRTAEEAGTLGLLLHKLFQRSFSVAKEVRSQTDIGACSIS
                     MAAAAVRVAERIFPAIAEQQLLLIGAGEMIELCATHFAAQKPKRITVANRTLERAQTL
                     AQRLGAGTITLNELPEQLAVNDIIVTGTASPLPILGKGMLERAIKARKHRPLLLIDLA
                     VPRDVEAEAGSLDDVFLYTVDDLGNMVQSGLGVRHSAVAQAEAIIDSNVTNFVHWLES
                     RELVPTIRALRDHAERSRRKELQRALRQLQRGADPQQVVEQLSQALLNKFLHVPTHTL
                     NHAGA"
BASE COUNT          880 a         1315 c         1301 g          879 t
ORIGIN      
        1 gtttgtagcg gtgcgtgtgg tggtggacac cgccgacatg acctttcccc gcagcgcaat
       61 ccgcgccagc gacgagtacg ggcgcctgca tatcaccaaa cttttattct ccctgcttgc
      121 ccaccctgcg gatcttagcg cgctgatcgg gttgagcaag agcttccgcg cggcgcaggc
      181 gacgctgcaa accatcgccc ggctcgctgg cccgaaatta tgcttgcagt agctattagc
      241 gctgtcacat gaccagcctg gttaccggcg cttcgggttt tgtcggctcg gcgctcgcgc
      301 gcagactcgt cgccgcggga caccgcgtga aggtcttgct gcgccccacc agcgaccacc
      361 gcaacatcga ggaattgccc gtggaaatcg cgcagggcga tctcaccgat aaagcctcgc
      421 ttgagcgcgc attagaaggc tgtgattcgc tgtttcacgc cgccgccgat taccgcctct
      481 ggacgcgcaa ccccgatgcg ctttatcaaa gcaacgtcag cggcacgctt aacatcatgc
      541 gggtagcgct cgagaccggc gtgaaacgca tcgtctatac cagcagtgtc gcgactttgg
      601 gcttgaatgc cgacggcacg ccagcggatg aaaatacgcc ggtgacgttc gccgacatga
      661 ttgggcatta caaacgctcg aaattcctag ccgaggcgga agtgaaaaag ctggtgcagg
      721 agcagggctt gcccgcggtc atcgtcaatc cctccacgcc ggtcgggccg cgcgacatca
      781 agcccacgcc tactgggcgc atggtgctgg atgcagcctg cggtcgtacg ccggcttatg
      841 tggacaccgg cctcaatctg gtgcatgtgg acgatgtcgc ggccgggcat tggctggctt
      901 atgagcgcgg caaaatcggc gagcgctaca tcctgggcgc acgcaacatg acgctcaagg
      961 aaattttgac cgtagtggcg aaaattgccg ggcataaacc acccagcgtg aggctgcccc
     1021 acaatctggt tttgccgatt gcttatcttt ctgaagcttg ggcgtggctc accgatggga
     1081 gcgagccgcg cgcgaccgtg gagggcgtgc ggctttccaa aaaatacatg tttttttcga
     1141 ttgagaaggc gcaacgcgaa ctgggctacc atccgcgggc ggtggaacaa gcgctgcaag
     1201 acgcggtgga ttggttccgc gccaacggct attgcggcta actccgcttc gcgtacctcg
     1261 ttcgcacgta gtcgtcgacg attttctgga attcctcggc gatattcgcg cccttgagcg
     1321 tcactgtttt ttgcccgtca acgaacaccg gcgccaccgg cgtctccccc gagccgggca
     1381 ggctgatgcc gatgttggcg tgcttgctct cgcccggccc gttcaccacg cagcccatca
     1441 ccgctacgtt cattttttcc acgccggcat atttctcgcg ccatgccggc atctggttgc
     1501 gcagataggt ttgaatcttg tcggcgagtt cctggaaaaa ggtgctggtg gtgcggccgc
     1561 aaccggggca ggccacgacc atcggcgtga acgagcgcaa tcccatcgtc tgcaggattt
     1621 cctgcgccac gacgacttcc cgggtgcggt cgccgccggg ctcgggcgtg agcgaaatgc
     1681 gtatcgtgtc gccgatgccc tgctgcaaca aaaccgagag cgccgcggtc gaagcaacga
     1741 tgccctttga gcccattcct gcttcggtga ggcccaggtg cagcggataa tcgcagcgcc
     1801 gcgccagttc cgagtacacc gcaatcaagt cctgcacgcc gctgaccttg caggagagca
     1861 cgattttgtc gtgcgcaagg ccgatttcct ccgcgcgcct ggcgttgtcg atcgccgaaa
     1921 caatcagcgc ttcgcgcgtg acttcgttgg cgtccttggg ttcgggaagt ttcgcgtttt
     1981 catccatcat gcgcgtgagc agtgccgggt cgaggctgcc ccagttcacg ccgatgcgca
     2041 ccggttttcc gtatttgcac gcgacctcga tcatggtggc gaactgctca tcgcgcttgg
     2101 aaccgcggcc gacgttgccg gggttgatgc ggtatttgga aagcgcctcg gcgcagccgg
     2161 gatattcggt gaggagcttg tggccgttga aatgaaaatc gccgatcagc ggcgccttac
     2221 agcccatttg ctccatgtgt ccgcggatgg cgggcacggc cgccgctgct tcggcggaat
     2281 tgacggtgat gcgtaccagt tccgaaccgg cttgggccag tgccgctacc tgtttggccg
     2341 tggactcgac gtcggcggta tcggtattgg tcatcgactg cactacgatg ggagcgccgc
     2401 cgccgatggt gactgggccg atgcgcacac cggcgctcac tcgtctggaa atcgtttgta
     2461 gagacataat ccttaaaaaa ccctaaactt gcgaaggcaa gccgcaaaga aaagtgaatc
     2521 caaacaacga attttaccgt atttgggagc agtcggcata agtttaggcg tgcgacccgg
     2581 cgagaatcat gtcaactcaa cccattatgg gcacgttact gttaggaaat gcagggcttt
     2641 ctcgacaaag aggttaatct tcagaattca tcaatgcgca gcccggtcgc atgatggaca
     2701 gcccggcttg tctgccggac ttaaaaaccg gtttttgcaa cttcaggaga cttaaaccat
     2761 gactgctgtt aaaaaactca caggtattgc tttggctgtt gctgcggccg gcatgtttgt
     2821 tactgccggt atcggcagcg ccgttgcggc agaaggcaaa gtgcactgtg aaggcgtgaa
     2881 cgcttgcaag ggcaagaccg actgcaagac tgccaagaac gcttgcaaag gccaaaacgc
     2941 ctgcaagggc aaaggctacg tcgagttgag cgaaaaagat tgccaggccg ccaaagctaa
     3001 ggccaaaaaa ggctaggact ccgcagttca tcacccaacg gacacctttg ccttaaccgg
     3061 ctggtgtccg ttttcttttt ccagcgcgga tgggcgggcc ctgaccaata cctgcgtgcc
     3121 gagcttttcc caaacgcccc ggtgctataa tacttttcgc tttttctgcc actacgagtc
     3181 gcggcatgca gttatttgcc tttggcatca accacaaaac cgcccccctg gccatgcgcg
     3241 agcaggtggc gtttcacgcg caaactttgg gtgtagcctt gcgcgacctg gttaaccgcc
     3301 ggccggttgc ggaagcggcc atcatttcca cctgcaaccg cactgaagtg tattgcaaca
     3361 cacccgagcc tgaagcggca gtcgaatggc tgtcggacta ccaccatctc aagactcaga
     3421 atatccagcc ttatctttac actctgccgc aggaaaaagc ggtgaagcac gctttccgcg
     3481 tcgccagcgg cctcgattcg atggtgctgg gcgagccgca gatcctgggg caaatcaaac
     3541 aagcggtgcg caccgcggaa gaagcgggaa ctttgggcct gttgttgcat aaactctttc
     3601 agcgctcgtt ttcggtggcg aaagaagtgc gcagccaaac cgatatcggg gcgtgttcga
     3661 tttccatggc cgccgccgcc gtgcgcgtgg ccgagcgcat ctttcccgca attgccgaac
     3721 aacaactgct cctgatcggc gcaggcgaga tgattgaatt gtgcgcaacc cacttcgcgg
     3781 cgcagaaacc gaagcgcatc acggtggcca accgcacgct ggagcgcgcg caaacgctgg
     3841 cgcaacgcct cggcgccggg accataacgc tcaatgagct gcctgagcag ctggccgtca
     3901 acgacatcat cgtcaccggc accgccagcc cgttgccgat attgggcaag ggcatgctgg
     3961 agcgggcgat caaagcgcgc aagcatcggc cgctgctcct cattgatctt gccgtgccgc
     4021 gcgatgtcga agccgaagca ggtagtctcg acgatgtgtt cctctatact gtggacgacc
     4081 tcgggaacat ggtgcaaagc ggcctggggg tgcgccatag cgcggtggcg caggcggaag
     4141 cgatcattga ttccaatgtc acgaatttcg tgcactggct ggaaagccgg gaactggtgc
     4201 cgaccatccg cgcgcttcgc gaccacgccg agcgcagccg cagaaaggaa ttgcagcgcg
     4261 cgctcagaca gcttcagcgc ggcgccgacc cgcagcaggt ggtggagcaa ttaagccagg
     4321 cgctgctcaa caagttcctg catgttccca cccacacgct gaaccacgcg ggagc
//
LOCUS       DATIOW010000021        10799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1734363, whole genome shotgun sequence.
ACCESSION   DATIOW010000021 DATIOW010000000
VERSION     DATIOW010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10799
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1734363"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /gene="ntrC"
                     /locus_tag="VLL03_00530"
     CDS             <1..1274
                     /gene="ntrC"
                     /locus_tag="VLL03_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HSS45890.1"
                     /translation="SAAPQVVVSDIRMPGGSGFELLQTLKQRFPQLPVIIMTAYSDLD
                     SAVAAFQGGAFEYLPKPFDVDHAVELIRRAMQQLSSEEDEAETAKAPEILGQAASMQE
                     VFRAIGRLSHSQATVLITGESGTGKELVACALHRHSPRAQKPFVAINTAAIPKDLLES
                     ELFGHERGAFTGAQSMRRGRFEQADAGTLFLDEIGDMPADLQTRLLRVLSDGQFYRVG
                     GHQPIKANVRVIAATHQDLEQRAKQGLFREDLFHRLNVIRLRLPSLRERRDDIPLLAR
                     HFLQKSARELSVEPKRLSDACLKFLAAQDFPGNVRQLENLCHWLTVMAPGQNIEVKDL
                     PPELRAVSGVQETDWSGALEKEVERALNRGDKAIMDELTRQFEKTLISKALAHTGGRR
                     IEAANLLGLGRNTLTRKIQELELEEKPDKTD"
     gene            complement(1271..2032)
                     /gene="xth"
                     /locus_tag="VLL03_00535"
     CDS             complement(1271..2032)
                     /gene="xth"
                     /locus_tag="VLL03_00535"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HSS45891.1"
                     /translation="MRLAAWNVNSLKVRLPQVLEWLSAQKPDLLCLQETKLEDANFPA
                     GEIRAAGYHSLFTGQKTYNGVAVLSRQAGGDIVAANPEFSDEQKRVLATTVDGVRVIC
                     VYVPNGQNVDSDKYRYKLDWLAALKEWLQMELKRHPRLVVLGDFNVAPEDRDVHDPKA
                     WEGNVLVSPRERQAFRELLDLGLKDSFRLFEQPEKSFSWWDYRLNAFKRNLGLRIDHI
                     LLNDTLAASCKSSTIDITPRKHERPSDHAPVLAEI"
     gene            complement(2032..2583)
                     /locus_tag="VLL03_00540"
     CDS             complement(2032..2583)
                     /locus_tag="VLL03_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HSS45892.1"
                     /translation="MKLRFLFCLLLFSASMGLAGNLYRWVDGEGNVHYSDQPPPPSVK
                     EVQEKTLGVSVIEGSQPYGLRQAVKNFPVTLFVSDCGAGCTRARELLNKRGVPFTEKN
                     PNQPENADELKKIVGDLVVPVLVVGNTQPLRGFEESSWNNALNFAGYPSAPVPGAPSG
                     GRTSGPQPSAAPAPAPTPESKPY"
     gene            complement(2698..4887)
                     /locus_tag="VLL03_00545"
     CDS             complement(2698..4887)
                     /locus_tag="VLL03_00545"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HSS45893.1"
                     /translation="MNPLLDFSGLPRFSQIEPQQIAPAVDALLAENRALVEKLVADPA
                     PAGWNNFVEPLEDANERLSRAWGPVAHLNSVMNSPELREAYNANLPKITQYNSELAQH
                     QGLFEKYQALKTLNSFPQLSHVQQKVVENELRDFRLGGAELPAEKKARFMQIQEELAH
                     LYSRFEENLLDATNDFALYVTNREELAGLPEDVLDAAKEAAAQDSKPGWKLTLHAPSW
                     IPVMQYADNRTLRERLYRAYVTRASEFSKAPATRCCETAGAGAGAPAPNQESSHGDEA
                     RLPAPAFSEGAESGAQWDNTPLIAQILKLRQEKARLLGYANYAEVSLARKMARSPREV
                     LDFLRELGKEARPYAERDFTELKQFARGELKLEILETWDIAYASEKQRAKRYSFSDQE
                     VKQYFPEPQVLQGLFQLVERLYGIRIEKTETPVWHPDVRFYNIFDFGGRLIGQFYVDL
                     YSRPSKRGGAWMDEAIARRRKDGAIQIPVAYLNCNFSAPVGGRPTLFTHDEVITLFHE
                     FGHGLHHLLTSVEELGVSGINGVEWDAVELPSQFMENFCWEWDVLKYLTRHVDSGEPL
                     PRALYEKMLAAKNFQSGMQTVRQLEFALFDMHLHYDFDPNGGKTPLQLLEEIRREVAV
                     VFPPHYNRLPNNFSHIFAGGYAAGYYSYKWAEVLSADAYSLFEENGVVNQNIGRRFWQ
                     EILSVGGSRPALESFVAFRGRAPKIDALLRHNGMSHSYKPGGCEQGE"
     gene            complement(4952..6226)
                     /locus_tag="VLL03_00550"
     CDS             complement(4952..6226)
                     /locus_tag="VLL03_00550"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HSS45894.1"
                     /translation="MKIHIKNGRVIDPKNGVDAIQDLFIAAGKVQTIGRTPESYHANR
                     VIDATNLIVCPGLIDLSARLREPGFEYKATLESEMQAAVAGGVTSLACPPDTDPPLDE
                     PGLVEMLKHRAKSLNQARVYPLGALTQGLNGERITEMAELHDAGCVAFSQANAPLNDT
                     DVLMRAMQYAATFGYTVWLRPQDAYLARGGVAHDGEVATRLGLPAIPSCAETIALATI
                     LLLARQTGAKIHLCRLSTAESVEMVRQAKAQGLPITCDIAAVHAHLSEMDLGFFDSNC
                     NLIPPLRSLRDRDALRSGLKNGVIDALCSDHTPVDDDAKLLPFAEAESGATGLELLLP
                     LTLKWANETQVPLSQALFKITHVPAKILGIDAGHLSPGHAADMCIFDPHHDGKIEANT
                     LKSQGKNTPFLGYELPGKVRFTLVEGHIVYEA"
     gene            complement(6310..7260)
                     /locus_tag="VLL03_00555"
     CDS             complement(6310..7260)
                     /locus_tag="VLL03_00555"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HSS45895.1"
                     /translation="MHSNPQLNQNGELQHLLTIEGLPATLLTRILDTAQSFIGVTERE
                     VKKVPLLRGKAIFNLFFEASTRTRTTFEIAAKRLSADVINLNISTSSQSKGETLLDTI
                     DNLEAMHADMFIVRHAQSGAAHLIARHVAPNVHVINAGDGRHAHPTQALLDMFTIRHY
                     KRSFTNLRVAIVGDILHSRVARSQIHALTTLGVPEVRVIAPKTLLPAGVEKLGVQVHH
                     DMRQGLKDVDVVMMLRLQNERMQGPLLPSAQEFFKYYGLTSDKLALARPDAIVMHPGP
                     MNRGVEIDSSVADGPRSVILPQVTFGIAVRMAVMSMLAGS"
     gene            complement(7315..7809)
                     /gene="pyrR"
                     /locus_tag="VLL03_00560"
     CDS             complement(7315..7809)
                     /gene="pyrR"
                     /locus_tag="VLL03_00560"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HSS45896.1"
                     /translation="MNLPDAEQLLNQLAAQIRPRLRAETALIGIHSGGAWIAQRLHEQ
                     LKIATPLGFLDISFYRDDFARIGLHPQVKPSHIPFDVEGRSIILVDDVLYTGRTVRAA
                     MNELFDYGRPASIDLAVLVDRGGRELPVFARYSGASLELADSQNIELKKDKNGRLSLS
                     LNQT"
     gene            complement(7796..8191)
                     /gene="ruvX"
                     /locus_tag="VLL03_00565"
     CDS             complement(7796..8191)
                     /gene="ruvX"
                     /locus_tag="VLL03_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HSS45897.1"
                     /translation="MAFDYGEKRIGVAVGDLSVRIAHPLTTIEATQAAKRLDAITDLV
                     EEWRPALIVVGLPSHMNGAEHEMSKRCRKFARRLEQRFRLRTALVDERLSSHAAELSL
                     RESGAAQTKTRIDQLAAQQILQGYFDELA"
     gene            complement(8325..8885)
                     /locus_tag="VLL03_00570"
     CDS             complement(8325..8885)
                     /locus_tag="VLL03_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HSS45898.1"
                     /translation="MSSVNLTNHFLIAMPNMADPYFSKSLTFICEHNEQGALGVVVNR
                     PIDMTLQALLDQISIPLGEPRLKGIPVHFGGPVQIDRGFVLHRPLGAWQSTLAVNSEI
                     GLTTSKDILQAVARGEGPQHILVTLGYAGWAPGQLEHELTQNAWLSVPANPDLMFNLA
                     AEERLAAAMELLGVNFANLSEDAGHA"
     gene            9025..9348
                     /locus_tag="VLL03_00575"
     CDS             9025..9348
                     /locus_tag="VLL03_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS45899.1"
                     /translation="MAHTFEELKGKTVAELRQIASGIEHEAVQGYTQLNKDHLLAALC
                     KALNIDMHAHHQVVGVNKTAIKAQIKALKQKRDEAMATHDHSRLKLIRRTIHHFKRQL
                     HKATV"
     gene            complement(9443..>10799)
                     /locus_tag="VLL03_00580"
     CDS             complement(9443..>10799)
                     /locus_tag="VLL03_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS45900.1"
                     /translation="VRLTAKELNKKANLEIRGSQVELDRSVLEKIVAPIEHMLRNSIA
                     HGIEQPNVRVAGGKFETGEINITLRQEANEIVITLSDDGAGLDFERIRQKGLELGLLQ
                     PGEQPDEARLAQLIFASGFSTAQEVTELVGRGVGLDVVEAEITGLSGRVDVASRHNQG
                     TTFTLHLPLTLAVAQAVLVRAAGRIYALPSTMVEQVREPRPEELATLQKNRELIWLGN
                     SYPFYYLPRLLGDNETMPETKRYNPVLLLRSGMQRTAILVDEIIRNQEVVVKNIGAQL
                     AHAPGVVGATVLGSGLVVLIINPVLLTAKLAAALAEAPQVVALDRVKEERAPLIMVVD
                     DSLTVRKITGRLLAREGYQVATAKDGVDAMQQLQDLIPDAMLVDVEMPRMDGFDLTKN
                     LRANPKTAHIPVIMISSRSVDKHRKLAVQLGVSVFLGKPYQEEELLKHIAGYVRQAAT
                     T"
BASE COUNT         2097 a         3159 c         3240 g         2303 t
ORIGIN      
        1 gttccgccgc gccgcaggtg gtggtatcgg acatccgcat gccgggtggc tcgggcttcg
       61 aacttctgca aacgctcaag cagcgtttcc cgcaactccc cgtcatcatc atgacggctt
      121 attccgattt ggacagcgcg gtggcggcgt tccagggcgg cgcgttcgag tatctgccca
      181 agccgttcga cgttgaccat gcggtggaat taatccgccg cgccatgcag cagctttcgt
      241 ccgaagagga tgaagcggag acggcgaagg ctccggaaat tctcggccag gcggcctcga
      301 tgcaggaagt atttcgtgcg attggcaggc tttcccactc gcaggcgacg gtgctgatta
      361 cgggcgaatc gggaaccggc aaggagctgg tggcgtgtgc tttgcatcgc cacagcccgc
      421 gcgcgcaaaa accgttcgtc gcgatcaata cggccgccat ccccaaggat ttgctggaat
      481 ccgaattgtt cggccatgag cgcggcgcgt ttaccggcgc gcagagcatg cgccgcggcc
      541 gcttcgagca ggccgacgcc gggaccctgt ttcttgacga aatcggcgac atgcccgccg
      601 atttgcagac ccgcttgctg cgggtgctat ccgacgggca attttatcgc gtgggcgggc
      661 accagccgat caaggccaac gtgagggtca tcgccgccac tcaccaggac ctcgagcagc
      721 gggcgaagca agggttgttt cgcgaagatt tattccaccg cctgaacgtg attcggcttc
      781 gcctgccgtc cttgcgcgaa cggcgcgacg atattccgct gctcgcgcgg cattttttgc
      841 aaaaaagcgc gagagaactc tcggtagagc cgaaacggct ttccgatgcc tgcctcaaat
      901 ttctcgctgc ccaggatttt ccgggcaatg tgcggcagct ggaaaacctc tgtcactggc
      961 tcacggtgat ggcaccgggg cagaacatcg aagtgaaaga cctgccgccc gagttgcgcg
     1021 cggtgagcgg cgttcaggaa accgactggt ccggcgcttt ggaaaaggaa gtggagcgtg
     1081 cgctcaaccg cggcgacaag gccatcatgg atgaactcac ccggcagttc gagaaaacgc
     1141 tcatcagcaa ggcgcttgcg catacgggcg ggcggcgcat cgaagcggcc aatttgctgg
     1201 gacttggccg caataccctc acccgcaaaa tccaggagct ggagctcgag gaaaagccgg
     1261 ataaaactga ttaaatttct gccaggaccg gcgcgtggtc ggaggggcgc tcatgcttgc
     1321 gcggcgtgat atcgatggta ctggatttac acgacgcggc gagcgtatcg tttaacagga
     1381 tgtgatcgat gcgtaagccc agattgcgct tgaacgcgtt caggcggtaa tcccaccagc
     1441 tgaaggattt ttccggctgc tcaaacaggc ggaagctgtc cttgagtccc agatccagca
     1501 gttcgcgaaa ggcctgccgt tccctggggc tcaccagcac attgccttcc caggctttgg
     1561 ggtcgtgcac atcgcggtct tccggcgcga cattgaaatc gcccagcacc acaagccggg
     1621 ggtggcgttt gagttccatt tgcagccatt ctttcaatgc ggccagccag tcgagcttgt
     1681 agcggtattt gtccgagtcc acgttctgcc cgttgggcac gtaaacgcaa atcacgcgca
     1741 cgccatcaac ggtagtggcc agcacgcgct tttgctcgtc gctgaattcc ggatttgcag
     1801 cgacgatatc cccgccggcc tgccggctca aaacagcgac gccgttgtag gttttctgcc
     1861 ccgtgaataa agaatggtaa cccgccgcac gaatttcccc ggccggaaag ttggcgtctt
     1921 caagcttggt ttcctgcagg cacagcaaat cgggtttttg cgcggagagc cactccagaa
     1981 cctgcggcaa gcggactttc agcgaattga cgttccaggc ggcaagccgc atcaataagg
     2041 cttggattcc ggtgtaggcg cgggcgccgg ggctgcgctt ggctgtggcc cggatgttct
     2101 gccgccggag ggcgctccag gaaccggagc tgaagggtaa cctgcgaaat tcaacgcatt
     2161 gttccagctg gattcttcaa agcctctgag cggttgagtg ttacccacga ccagcaccgg
     2221 caccaccaaa tctcctacga tttttttcaa ctcgtcggca ttctcgggct ggtttggatt
     2281 tttttcggtg aacggcacgc cgcgcttgtt cagtaactcg cgcgctctgg tgcatcccgc
     2341 gccgcagtcg ctgacgaaca gggtgacagg gaaattttta acagcctgcc gcaggccgta
     2401 gggttgactg ccttcaatca cgctcacgcc gagtgttttt tcctgaactt ctttcaccga
     2461 aggcggtggc ggctggtcgg agtaatgcac attgccctcg ccatccaccc agcggtagag
     2521 atttccggcc aagcccatgc tcgcggaaaa cagcaaaaga caaaataaga aacgaagttt
     2581 catagtgaat attttacctg ctatgacttt agcacagatt ggttcgcgct agaacgggtc
     2641 atgccgcctc catcattact cgcttgatcg ccacagggcc gcgtcggcgg ccaggcgtca
     2701 ttcgccttgt tcgcatcccc ccggcttgta tgagtggctc atgccgttat gccttagcag
     2761 ggcgtcgatt ttcggcgccc ggccgcggaa cgcgacgaag gattccagcg cgggacggct
     2821 gccgcccacg ctcaggattt cctgccagaa acgccggccg atattctggt tgaccacccc
     2881 gttttcctca aacaagctgt acgcgtcggc ggaaagcacc tccgcccact tgtagctgta
     2941 ataacccgcg gcataaccgc cggcgaagat atgcgagaaa ttgttgggga ggcggttgta
     3001 gtgcggcggg aaaaccaccg cgacttcacg gcggatttct tccagcaatt gcaacggggt
     3061 tttgccgccg ttgggatcga aatcataatg cagatgcatg tcgaacagcg caaactcgag
     3121 ctggcgcaca gtctgcatgc cgctctggaa attcttggcg gcgagcattt tctcgtatag
     3181 tgcgcgcggc agcggttcac cgctgtcgac gtgacgcgtc agatacttga gcacgtccca
     3241 ctcccagcag aaattctcca tgaactggct gggaagctcc accgcgtccc attccacgcc
     3301 gttgatgccc gaaacgccca gctcttccac cgaagtcagc aggtgatgca agccgtggcc
     3361 gaattcgtga aacagcgtaa tcacctcgtc gtgggtaaac agcgtgggcc tgccgcccac
     3421 cggcgcggag aaattgcagt tgagataggc cacgggaatt tggatcgccc cgtccttgcg
     3481 ccgccgcgca atcgcttcat ccatccaggc gccgccgcgc ttcgacggcc gcgaataaag
     3541 gtcaacgtaa aactgcccaa tcagcctgcc gccaaaatcg aagatgttat agaaacgcac
     3601 atccggatgc cataccggcg tttcggtttt ctcgatgcga atgccgtaaa gcctttccac
     3661 cagctgaaac agtccctgca acacctgggg ctcgggaaaa tactgtttca cttcctgatc
     3721 ggagaacgaa tagcgtttgg cgcgctgctt ttccgaggcg tacgcgatgt cccatgtctc
     3781 aagtatttcg agcttgagtt cgccgcgtgc gaattgcttg agttcagtga aatcacgctc
     3841 ggcgtagggt cgggcttctt tcccgagttc gcgcaggaaa tccagcacct cgcgcgggct
     3901 gcgcgccatt ttgcgcgcta gagaaacttc cgcgtaattg gcatagccca aaagccgcgc
     3961 tttttcctgc cgcagtttca ggatttgcgc aatcagcgga gtgttgtccc attgcgcgcc
     4021 cgactcggcg ccttcggaga aggccggggc ggggaggcgc gcctcgtcac cgtgggaact
     4081 ctcttgattc ggcgctggtg cgcccgctcc cgcacccgct gtttcacagc accgtgtcgc
     4141 gggcgctttg ctaaactccg aggcgcgcgt gacgtaagcc cggtacagcc gctcccgcaa
     4201 ggtccggttg tccgcatatt gcatcaccgg aatccaggaa ggcgcgtgca gggtgagctt
     4261 ccagcccggc ttgctatcct gggcggcggc ttcctttgcg gcatccaaca cgtcttcggg
     4321 caggcccgcc agctcttcgc gattggtcac gtaaagcgcg aaatcgttgg tggcatcgag
     4381 caggttttcc tcgaatctgg aatacagatg cgccagttct tcctggattt gcatgaagcg
     4441 cgcttttttc tccgcgggca gttcggcgcc gcccaagcgg aaatccctaa gttcattttc
     4501 caccactttt tgctgcacgt ggcttaattg gggaaaacta ttcagggttt tgagcgcctg
     4561 gtatttctca aacagccctt gatgctgcgc aagctcgctg ttgtattggg tgattttggg
     4621 gagatttgcg ttgtaagctt cgcgcagttc cgggctgttc atcaccgagt tgaggtgagc
     4681 gacgggtccc caggcgcgcg acaagcgctc gttggcgtcc tccaggggct cgacaaaatt
     4741 attccagcct gccggcgccg gatcggcgac cagcttttcc accagcgcac gattttccgc
     4801 cagcaaggca tcgaccgctg gggcgatctg ctgcggctcg atctgagaaa agcgcggcaa
     4861 gcccgaaaaa tcaagcaaag gattcatagg gttaaatccg ggagcggggt ctagggacgg
     4921 tgcttatttt acacccgctg agaatcgtag gctaagcctc gtagacgatg tggccttcga
     4981 ccagcgtaaa gcgcaccttt ccgggaagct catagccgag gaagggtgtg ttttttcctt
     5041 gactcttcaa tgtattagct tcaatcttgc catcgtgatg cgggtcgaaa atgcacatgt
     5101 cggcggcatg gcctggactt aaatgcccgg cgtcgatgcc gagaatcttc gccggcacgt
     5161 gggtgatttt gaaaagcgcc tgggacagcg gtacctgggt ttcgttggcc catttcaggg
     5221 tgagcggcaa caggagttcc agtccggtcg cgccgctttc cgcttcggca aacggcagga
     5281 gcttggcatc gtcatccact ggcgtgtgat ccgaacacag cgcatcgatc acgccgttct
     5341 tgagcccgct gcgcaacgcg tcacggtcgc gcaaggatcg cagtggcggg atcagattgc
     5401 agttggaatc gaaaaagccc agatccattt cactcaagtg cgcgtgcacg gcggcgatat
     5461 cacaggtaat cggcagaccc tgcgctttgg cctgccgcac catttccacg ctttctgcag
     5521 tggagagccg gcacagatgg atttttgccc cggtttgcct cgccagcagc aggatggtgg
     5581 caagcgcgat ggtctcggcg caactcggaa tcgcgggaag tccaagccgt gtggccactt
     5641 cgccgtcatg tgcgacaccg ccgcgcgcaa gatacgcgtc ctgcgggcgc agccatacgg
     5701 tgtagccgaa agtcgccgca tattgcatcg cacgcatcag cacgtcggtg tcgttgagcg
     5761 gcgcattcgc ttgcgagaat gccacgcagc cggcatcgtg cagttcggcc atttcggtga
     5821 tgcgctcgcc atttaaccct tgagtcagtg caccaagggg atagacgcgc gcctgattca
     5881 ggctcttggc gcggtgcttg agcatttcca ccagtcccgg ttcatccagc ggtggatcgg
     5941 tatccggcgg gcaggccaga ctggttacgc cgccagcgac cgccgcctgc atttccgatt
     6001 ccagcgtcgc cttgtattcg aaacccggct cgcgcaggcg cgccgaaaga tcgatgagtc
     6061 ccgggcagac aatcaggttg gtggcgtcga tgacgcggtt ggcatgataa ctctcaggcg
     6121 tgcgccctat cgtctgcacc ttgccggcgg cgatgaacaa atcctgaatg gcgtccacgc
     6181 cgtttttggg atcgatcacg cggccgtttt taatgtgaat tttcatttta aagctttcag
     6241 cattcagctg tcggcggtca gcttaagcct gttttggccg aatgctgagg gctgatagct
     6301 gacagcgttt caactccccg ccagcatgct catcaccgcc atgcgcactg cgatgccgaa
     6361 ggtgacttgc ggcagaatca ccgaacgggg tccgtcggcc acgctggaat cgatttctac
     6421 gccgcggttc atcgggcccg gatgcatgac gatggcatcg ggtcgggcca gcgccagttt
     6481 gtccgaagtc aagccgtagt atttgaagaa ttcctgcgcg ctcggtagca gcgggccttg
     6541 catgcgttca ttctgcagcc tgagcatcat caccacgtcc acgtccttca ggccctgccg
     6601 catgtcatgg tgcacctgca ccccgagttt ttccacgccc gcgggcagca gggttttcgg
     6661 cgcgatgacg cgcacttcgg gcacgccaag ggtggtgagg gcgtggattt gcgagcgcgc
     6721 cacccgtgag tgcaggatgt cgccgacaat tgccacccgc aggttggtga aactgcgctt
     6781 gtaatggcgg atggtaaaca tgtccaggag cgcctgcgtc gggtgggcgt ggcggccgtc
     6841 gccggcgttg atcacgtgca cgttcggcgc gacgtgacgg gcgatcaggt gcgccgctcc
     6901 gctttgcgca tggcgcacga tgaacatgtc ggcgtgcatc gcctccagat tgtctatggt
     6961 gtcgagcagg gtttcgccct tgctctgcga ggaggtgctg atattgaggt tgatgacgtc
     7021 ggcggagagc cgcttcgccg cgatttcaaa agtcgtgcgg gtgcgggtgc tggcttcgaa
     7081 gaagagattg aatatcgctt tgccgcgcag cagcggaact ttcttgactt cgcgctcagt
     7141 gacgccgatg aaggactgcg cggtgtcgag aatgcgggtt agaagtgttg ccggcagccc
     7201 ttcgatcgtg agtaagtgct ggagttcgcc gttttggttg agctgcggat tgctgtgcat
     7261 gtacgcgcag gcaggattat agcaccattg gcgaaagcct gcccgcccgc ggagctaggt
     7321 ctggttcagg cttaggctca aacgcccgtt cttgtccttt ttgagttcga tgttctggct
     7381 gtcggcaagc tccaggctgg cgccgctgta gcgcgcgaat accggcaact cgcgcccgcc
     7441 gcggtccacc agcaccgcca ggtcaatgct ggccggccgg ccgtaatcga aaagctcgtt
     7501 catcgccgcg cgcacggtgc ggccggtgta gagcacgtcg tcgacaagaa tgatgctgcg
     7561 tccttcgaca tcgaagggta tgtgcgaagg cttgacctgc ggatgcaggc cgatgcgcgc
     7621 aaaatcgtcg cggtagaacg aaatgtcgag aaacccgagc ggagtggcga ttttgagctg
     7681 ctcgtgcagc cgctgcgcga tccaggcgcc gccactgtgg atgccgatga gcgccgtctc
     7741 cgcccgcagc cgcgggcgga tttgcgccgc gagctggttg agcaattgtt cggcatcagg
     7801 caagttcatc gaaatagccc tgtaaaatct gttgtgcggc cagctggtcg atgcgggttt
     7861 tggtttgcgc cgcgccgctt tccctgagcg aaagttcggc agcgtgggag ctcaagcgtt
     7921 catcaaccaa agcggtgcgc agcctgaacc gttgttccag ccgccgcgcg aacttgcggc
     7981 agcgcttgct catttcgtgc tcggcgccgt tcatgtgcga cggcaggccg actactatga
     8041 gcgcaggccg ccattcttcg accaggtccg taatggcatc caagcgtttg gcagcctgcg
     8101 ttgcttcgat ggtggtgagc ggatgggcaa tgcgcacact caaatcgcct accgcgacgc
     8161 cgatgcgttt ctcgccataa tcaaaagcca aaaccgttcc ggttttggag tgagaggtaa
     8221 ggggtgaagg gtaaggggta aattttgttt ttaccacgtt gttgttttgc tcctcgctct
     8281 ccacattttc acttcaactc tcaactcata actcttaact gatctcaagc atgccccgca
     8341 tcttccgaca aattggcgaa attgacgccc agcaattcca tcgccgcggc caggcgttct
     8401 tcggcggcaa ggttgaacat cagatcggga tttgccggca ccgaaagcca ggcgttttgc
     8461 gtaagctcat gctcgagctg ccccggcgcc catccggcat aacccagggt caccagaata
     8521 tgttgcgggc cttcgccgcg cgccaccgcc tgcagaatat ccttggaagt agtgaggccg
     8581 atttccgaat tgacggcgag cgtggattgc caggcgccga gcggccggtg caatacgaag
     8641 ccgcggtcaa tctgcaccgg tccgccgaaa tgcacgggta tgcctttcag gcgcggttcg
     8701 cccaacggga tgctgatttg gtcaagcagc gcctgcagcg tcatgtcaat cgggcggttg
     8761 accaccacgc cgagcgcccc ctgttcgttg tgctcgcaaa taaaagtcag gcttttggaa
     8821 aaatagggat ccgccatgtt aggcatggcg atcaggaaat gattggtcaa gttgacggac
     8881 gacatggatc tattttaacc tccccgtcct gccttcacaa tgaagttccg cggttgccgc
     8941 atactggttt cgatgggcaa aacagggaaa tattcttcga gacgcgatac gatgtaaaat
     9001 tcggggatcc cctgaggagg aaacatggct cacacctttg aagaactgaa gggcaaaact
     9061 gtcgccgagc ttcgccagat tgcttccggg atcgaacacg aggcggtgca aggctacacg
     9121 cagcttaata aagaccattt gctggccgcc ttgtgcaagg cgctcaacat tgacatgcat
     9181 gcgcatcatc aggtggtggg ggtgaacaag accgccatca aggcgcagat caaagcgctc
     9241 aagcaaaagc gcgatgaagc catggccacg catgatcatt ccaggctcaa actaatccgg
     9301 cgcaccattc accacttcaa gcggcagcta cacaaagcca cggtataggc agttggtttg
     9361 ggttccgtcc atacgttccg tgccggacta tggccacgcc gcgcgcaaaa ccgcgctgga
     9421 aatgtacggc gaagagaccg gactaggtgg tggccgcttg cctgacgtag ccggcgatgt
     9481 gcttcagcag ttcttcttcc tgataaggct tgccgaggaa cacgctgacg ccgagctgta
     9541 cggcaagctt gcggtgcttg tccaccgagc gcgagctgat catgatgacg ggaatatgcg
     9601 cggtcttggg gttggcgcgc aggttcttgg tgagatcgaa cccgtccatg cgcggcattt
     9661 cgacgtccac cagcatcgcg tcgggtatca ggtcctgcaa ctgctgcatg gcatccacac
     9721 cgtccttggc agtcgccacc tgatagcctt cgcgcgcgag caggcggccg gtgatcttgc
     9781 gcacggtgag cgaatcgtcc accaccatga tgagcggcgc gcgttcttct ttgacgcgat
     9841 cgagtgcaac aacctgcggg gcttcggcga gagcggctgc tagcttcgca gtaagcagca
     9901 ccgggttaat aatcaacacc accagcccgc tgccgagcac ggtggcgccg accacacccg
     9961 gcgcgtgcgc cagttgcgcg ccgatgtttt tgaccaccac ttcctggttg cggatgattt
    10021 cgtccaccag gatcgcggtg cgttgcatgc cgctcctgag cagcagtacc gggttgtagc
    10081 gcttggtttc aggcatggtt tcgttgtcgc ccagcagccg cggcagataa taaaagggat
    10141 agctgttgcc cagccatata agctcgcggt ttttctgcaa tgtcgcaagc tcctcgggac
    10201 gcggctcccg tacctgctcg accatggtgg aaggcagcgc gtaaatccgg cccgcggcgc
    10261 gcaccagcac cgcttgcgcc actgccagtg tcagcggcag gtgcagggtg aacgtggtgc
    10321 cctggttgtg ccttgaagcg acatccacgc ggccgctcaa accggtaatc tcggcttcta
    10381 ccacgtccaa gcccacgccg cggccgacaa gctccgtcac ttcctgcgcg gtggagaagc
    10441 cggaagcaaa aatcagttgc gcaagccgcg cttcatccgg ctgttcgccc ggttgcagca
    10501 ggccgagctc cagacctttt tggcggatgc gctcgaaatc caaccccgcg ccgtcatcgc
    10561 tcaaggtaat cacaatttca ttggcttcct ggcgcagggt gatgttgatt tcaccggttt
    10621 caaatttgcc gccggcgacg cgcacgttgg gttgttcaat accgtgtgcg atggaatttc
    10681 tcagcatgtg ttcaatcggc gcgacaattt tctccagcac gctgcggtcc aactcgacct
    10741 ggctgccgcg gatttccagg ttggctttct tgttgagttc cttggcggtc agacgcaca
//
LOCUS       DATIOW010000022        10233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1734890, whole genome shotgun sequence.
ACCESSION   DATIOW010000022 DATIOW010000000
VERSION     DATIOW010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10233
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1734890"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VLL03_00585"
     CDS             complement(<1..191)
                     /locus_tag="VLL03_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS45901.1"
                     /translation="MEPRQVEAGRGWQWLVDGFALFRKNPLIWVVLCAILVAISYVLI
                     KIPFLGIVLNLILPVLVGGL"
     gene            complement(251..1195)
                     /locus_tag="VLL03_00590"
     CDS             complement(251..1195)
                     /locus_tag="VLL03_00590"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HSS45902.1"
                     /translation="MSVFTTVTREQLATWLRNYSLGMLTDLHGIAAGIDNTNYFVYTT
                     HGRYVLTLFEKLKAEELPYYLNLMSHLASHGIPCPNPIANLVNSFLGELNGKPACLVS
                     CLQGVDLKQPSPEHCAEVGELLADMHLAGRTYPDKMENPRGTHWWKATAPQIMPFLSQ
                     EEADLLKAELSYQSLYRYHDLPRGVIHADLFCDNVLFHNDAVTGVIDFYFACNDVLLY
                     DVAIAVNDWCITQNYELDVRRTRAFLSAYHSTRPFSAAEREAWLVMLRAGALRFWVSR
                     LQDFHLPRPGELTHAKDPNHFMKLLKQHASGQSGLVWL"
     gene            complement(1258..1593)
                     /locus_tag="VLL03_00595"
     CDS             complement(1258..1593)
                     /locus_tag="VLL03_00595"
                     /inference="COORDINATES: protein motif:HMM:NF022639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HSS45903.1"
                     /translation="MRLVTAALLCLALSAFAQAPQRPRDLQPLPEAPPPPPPPVGSPM
                     EPQVTIIKRGQETVEEYRVHGRLYMIKVTPSHGVPYYLLDESGQGKFVRHDDLDNPVK
                     VPLWLLLEF"
     gene            complement(1654..2340)
                     /locus_tag="VLL03_00600"
     CDS             complement(1654..2340)
                     /locus_tag="VLL03_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HSS45904.1"
                     /translation="MLEKSWSARESWRVFEIMAEFVEATERLSAIRPAVSIFGSARTP
                     QDHPYYKLAEQIARQLSDAGFAVISGGGPGIMDAANKGAFHGKSPSVGLNIQLPLEQH
                     SNAYQDISQTFRHFFARKVMFVKFATAYVVLPGGFGTLDELMEALTLVQTGKTRKIPI
                     ILVHEPFWRGMLDWFKQTLIAENMIDAEDMHLIKVINEPKAVVDAIFDHYEKYGFEPS
                     AQEREIQLNL"
     gene            2443..5211
                     /gene="polA"
                     /locus_tag="VLL03_00605"
     CDS             2443..5211
                     /gene="polA"
                     /locus_tag="VLL03_00605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HSS45905.1"
                     /translation="MKNATNADNGRKTLLLVDGSSYLYRAFHALPDLRNRRDEPTGAI
                     YGVLNMLRRLRSDYKADYSACVFDAKGKTFREDWYPEYKANRPAMPDDLAAQIAPLHE
                     CIRALGWPLLIIEGVEADDVIGTLVTMAAREGVNSIVSTGDKDLTQLVNLHVMLVNTM
                     SNEKLDEQGVLAKFGVAPARMVDYLTLIGDSVDNVPGVDKVGPKTAVKWLSQYRTLDN
                     LITHADEIPGVVGENLRKSLDWLPQARQLLTVKCDVDLPVALPDLASQPQDAAKLNEL
                     FERFEFKSWLRESKESGVRSEEPSQVGTHSAPQTAHPLPLTAHPSQRNYETILTEAQL
                     EKWLKRIHAAQLVAVDTETTGLDPMQDKLVGISFSVEANRAAYIPLTHRYSGAPEQLD
                     SGRVLERLKPWLKDAGKPKLGQNLKYDKHIFANHGAALNGIAHDTLLESYILESHKPH
                     DMDSLAERHLGVKTITYDEVTGKGANRINFDQVAIDRAAEYAAEDADVTLQLHQALYP
                     RIAADSRLEDIYRRIEMPLLEVLFSMERNGVLLDVELLDEQSRELGSRMLELENHVHQ
                     AAGQPFNLNSPKQIQEILFERQKLPVVKKTPGGQPSTDEDVLQELALNYPLPKLILDY
                     RGLAKLKSTYTDKLPRMVNPQTGRVHTNYAQAVAVTGRLASNDPNLQNIPVRTQEGRR
                     IREAFIAPPGHRIVSADYSQIELRIMAHISGDKSLLRAFAEGQDIHRATASEIFGIPP
                     AEVGPEQRRYAKVINFGLIYGMSAFGLAGQLGIERSAAQLYMERYFARYPGVAEYMQR
                     TRELAKQQGFVETVLGRRLWLPDIKSSNGTRRQGAERAAINAPMQGTAADLIKLAMIA
                     VHGWLKQKRLKAKLIMQVHDELVLEVPDDELELLKTELPKLMCGVAKLNVPLVVDVGT
                     GPNWEKAH"
     gene            5267..5785
                     /locus_tag="VLL03_00610"
     CDS             5267..5785
                     /locus_tag="VLL03_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSS45906.1"
                     /translation="MRAMNTLKNRRFLILLALGALLAVLFTQAVTQNSPAPQVTFTSL
                     KGEKIPMRSLRGKVVLVNFWATSCVGCVHEMPQMVDTYRKFQAQGLETIAVAMSYDPP
                     NYVVNYTEKNALPFKVALDVQGDIAQAFGDVKLTPTTFVIDKRGNIIKRILGEPDFAK
                     LHLLLEKELQTA"
     gene            5852..6376
                     /locus_tag="VLL03_00615"
     CDS             5852..6376
                     /locus_tag="VLL03_00615"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HSS45907.1"
                     /translation="MIGWKQHFIYQVDYQHWANEQIFIMLDRFDDNVRKSQQGTLSES
                     IHNSVDHMLVLTRVWFGRLKAQPNELPFNQLLHPDWRELKRAFRQELRDLQRWLETRP
                     EDFFAQQVSYKTGEGRAASFWVRDLLTHLMLQLIHHRGQVTSVAGKLGAPAPELGYLF
                     YRREMDKYLAQIRR"
     gene            complement(6373..7323)
                     /gene="lipA"
                     /locus_tag="VLL03_00620"
     CDS             complement(6373..7323)
                     /gene="lipA"
                     /locus_tag="VLL03_00620"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HSS45908.1"
                     /translation="MSSVAEERKQKGEAKTARIPVKIVPMARLKKPDWIRVKAASGTR
                     FYEIKRTLREHNLHTVCEEASCPNIGECFGKGTATFMILGDLCTRRCPFCDVAHGRPL
                     PPDADEPMHLAQTISVLKLNYVVITSVDRDDLRDGGARHFTDCIRCVREKSPHIRIEI
                     LVPDFRGRLEIALDILSACPPDVMNHNLETVPRLYKQARPGADYYNSLRLLKEFKARH
                     PEIPTKSGLMLGLGETDEEILEVMHDLREHNVDMLTLGQYLQPSAHHLPVLRYAEPAL
                     FNKFEQLASQMGFLHAACGPMVRSSYHADQQAHEAGIAAI"
     gene            complement(7320..7910)
                     /gene="lipB"
                     /locus_tag="VLL03_00625"
     CDS             complement(7320..7910)
                     /gene="lipB"
                     /locus_tag="VLL03_00625"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010922.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HSS45909.1"
                     /translation="MGPVEYLTAWQAMQDFTRQRDANTPDEIWLMQHPPVYTVGLNGK
                     SEHLPKRTTIPVIKTDRGGQITYHGPGQLVIYPLLDMRRLKLGVRPLVRSLEQSVIDL
                     LHDYGIEAQNRVDAPGVYVSGAKIAALGLRIKNGCCYHGLALNVDMDLTPFHAINPCG
                     HPGLQVTQLRALGIADSPDQVGNKLIQKLLHQLEKT"
     gene            complement(8015..8281)
                     /locus_tag="VLL03_00630"
     CDS             complement(8015..8281)
                     /locus_tag="VLL03_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HSS45910.1"
                     /translation="MGLEPSLIEYPCDFPIKIMGRSQAGFAQAVMEIVLRHAPDFDPA
                     SLEMKPSKKGKYLSLTCVIQARSREQLDDLYRELCDHPMVVMVL"
     gene            complement(8288..9163)
                     /locus_tag="VLL03_00635"
     CDS             complement(8288..9163)
                     /locus_tag="VLL03_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HSS45911.1"
                     /translation="MSRQIVYLNGKFLPPEEARVPVLDRGFIFGDGVYEVIPAYSRIP
                     FRLKQHLLRLQTSLGAIRLANPHSDAEWTRLIGELIAQNQGEDQYLYLHVTRGPAKRD
                     HAFPKQVHPTVFMMSTPLTIPPPELLKTGVAAITAQDKRWLRCDIKAISLLPNVLLRQ
                     MAADAGAAEAVLLRDGFLTEGAASNILVVKDNLLLAPPKNHLMLAGITYDVVLELAAA
                     NNIPHEVRVVSENEVRSAQELWMTSSTREVLAITILDGKPVGDGKPGEHFWRMHGLYQ
                     RYKETVMRKAEAPAI"
     gene            complement(9160..>10233)
                     /locus_tag="VLL03_00640"
     CDS             complement(9160..>10233)
                     /locus_tag="VLL03_00640"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HSS45912.1"
                     /translation="QLPVPQAPPVAAKSYLLTDFYSRQVLVRQNVNERIEPASLTKLM
                     TAYLTFTALKQKQITLNQTLPVSTRAWRAEGSRMFIEPNRPVTVDELIHGMIVQSGND
                     ACIALAEGIAGSEEVFAQMMNREAQRLGMKDTHFMNSTGLPHPQHYSTAHDLSLVAAA
                     IIRDFPEYYPLYSTKEYKYNNISQSNRNRLLWTDPYVDGMKTGYTENAGYCLISTAKR
                     GERRLISVVLGTSSDSVRATESQKLLNYGFQFYDTLHLYLKGQAITSFPVWKGAENSV
                     KAGFSNDVYLSLPKGQAERLKATMESRQPLIAPISAGQNVGVVKLTLDGKPIAEYPVV
                     ALESIGLANILGRGWDTLRLLFN"
BASE COUNT         2234 a         2830 c         2992 g         2177 t
ORIGIN      
        1 aggccgccca ccagcacggg caggattaaa ttcaacacta tcccgagaaa gggaatttta
       61 atcaagacgt aagaaattgc gacgaggatg gcgcacagga caacccaaat caaaggattt
      121 ttcctgaaca aagcaaagcc atcaaccagc cattgccagc cccggcctgc ttcgacttga
      181 cgcggttcca tgtctcacct cccaagcttg ttgtgtgcta cttccaactt acaccccttt
      241 aagatggatt tcaaagccag acaagcccgg attggccgct tgcgtgctgc ttcaataatt
      301 tcatgaaatg attggggtcc ttggcatggg tgagctcgcc cggccgcggc aaatgaaagt
      361 cctgcaggcg cgagacccag aagcgcagag cgcccgcgcg cagcatcacc agccaggcct
      421 cgcgttcggc tgcactaaac gggcgggtgg agtggtaagc ggagaggaaa gcgcgggtgc
      481 gtctgacgtc gagttcataa ttttgcgtga tgcaccagtc gttgacggca atcgccacgt
      541 catagagcag cacgtcgttg caggcgaaat agaaatcaat cacgccggtc accgcgtcgt
      601 tgtggaacag cacgttgtcg caaaacaaat cggcatggat tacgccgcgc ggtaggtcat
      661 ggtagcgata aagcgactgg tagctcagct cagctttcag cagatcggcc tcctcttgcg
      721 agaggaaagg catgatttgc ggcgcggtgg ctttccacca gtgggtaccg cgcgggtttt
      781 ccattttgtc cggataggtc cgcccggcga ggtgcatgtc ggccaagagc tcgcccactt
      841 cggcgcagtg ttcgggcgag ggttgtttca agtccacccc ctggaggcag ctcaccaagc
      901 acgccggctt gccgtttaat tcccccaaaa aactgtttac cagattggcg atgggattgg
      961 ggcaggggat gccgtggctc gccaggtgcg acattaaatt cagataatag ggcagttcct
     1021 cggctttgag cttctcgaac agcgtcagca cgtagcggcc gtgggtggtg tagacaaaat
     1081 aattggtgtt atcaattccg gcggcgatgc cgtgcaaatc tgtgagcatg cccagcgaat
     1141 aattcctgag ccacgtagca agctgctcgc gggtgacggt ggtgaagaca gacatgtgtt
     1201 gttgtcggcc gcggtattca tcccgcggct gcaaagggat ttataccggc ggccggatca
     1261 aaattccagc aacaaccaca gcggcacttt aaccgggtta tcgaggtcgt cgtgccggac
     1321 aaacttgcct tggccagact catccaacaa gtaatagggc accccgtgcg agggcgttac
     1381 cttgatcatg tacaaacggc cgtgcacccg gtattcctcg acggtctcct ggccgcgctt
     1441 gatgatggtg acctgtggct ccatcgggga gccgaccggc ggcggcggag ggggtggagc
     1501 ctcgggcaag ggttgcaaat ccctaggtcg ttgcggggcc tgcgcaaacg cggatagcgc
     1561 aaggcaaagc aaagcggcgg taacgaggcg catgcggtcc tttacgggtg gtttgcccgg
     1621 aacgttagag ggcgttagtt tagcattaag acatcagagg ttgagctgga tttcgcgttc
     1681 ctgcgcagaa ggctcgaagc cgtatttctc gtaatggtca aaaatcgcat cgaccaccgc
     1741 tttcggctcg ttgatgacct tgatgagatg catgtcttcg gcgtcgatca tgttttccgc
     1801 aatcagcgtt tgcttgaacc aatccagcat gccgcgccag aacggctcgt gcaccaggat
     1861 aatgggaatt ttgcgtgttt tgccggtttg cactagcgtc agtgcttcca tcagttcatc
     1921 cagcgtgcca aaaccgccgg gcaagaccac gtaggcggtg gcgaatttca cgaacatcac
     1981 cttgcgcgcg aagaaatggc ggaaggtctg gctgatgtcc tgataggcgt tgctgtgttg
     2041 ttcgagaggc agctgaatgt tcaggcccac gctcgggctt ttgccgtgaa atgctccttt
     2101 gttcgccgca tccatgatgc cggggccgcc gccggaaatc acggcaaagc cggcatccga
     2161 cagttgtcgc gcgatttgtt cggcgagctt gtaatacggg tggtcctgcg gcgtgcgcgc
     2221 gctgccgaaa atgcttaccg caggacggat cgcgctcaag cgttccgtcg cttccacaaa
     2281 ttctgccata atctcgaata cgcgccagga ttcgcgcgct gaccacgatt tctcgagcat
     2341 tccgggggct ataaatttct tgagcttgtc taaatgcgtc attttgaaag cctgttcagt
     2401 gagttcgaat cgtaactacg cccacatgcg gggcgggaga aaatgaaaaa cgccacgaac
     2461 gcggacaatg ggcggaaaac gctgctgctg gttgacggtt cttcgtatct ttaccgagcc
     2521 ttccacgcgc tgccggattt gcgcaaccgc cgggacgagc ccaccggcgc gatttacggc
     2581 gtcctcaaca tgctgcgccg gttgcgcagc gactacaagg cggattatag cgcctgcgtg
     2641 ttcgacgcaa aaggcaagac cttccgcgag gactggtacc ccgaatacaa ggctaaccgg
     2701 ccggcgatgc cggacgatct cgccgcgcaa atcgccccgt tgcatgaatg cattcgcgcg
     2761 ctcggctggc cgcttttgat catcgaaggt gtggaagcag acgatgttat cggcacgctc
     2821 gtcaccatgg ccgcgcgcga gggcgtcaac agcatcgtct ccaccggcga caaggacctt
     2881 acgcagctcg tcaatctgca tgtcatgctc gtcaacacca tgagcaatga aaaactggat
     2941 gagcagggtg tattggccaa attcggcgtg gcgcctgcgc gcatggtgga ttatctgacg
     3001 ctgatcggcg acagcgtgga caatgttccg ggcgtggaca aggtcggacc gaaaaccgcg
     3061 gtgaaatggc tctcgcagta ccggacgctc gataatctga taacacacgc cgatgaaatt
     3121 cccggcgtgg tgggcgaaaa cctgcgcaag tcgctcgatt ggctgccgca ggcgcgccag
     3181 ctccttaccg tcaaatgcga tgtggatttg ccggttgctc ttcccgacct tgcgtcacaa
     3241 ccgcaggatg cggcgaaact caacgagctg tttgagcgtt ttgaattcaa gagctggctt
     3301 agggaaagta aggagtcagg agtaaggagt gaggagccaa gtcaagttgg aactcattcc
     3361 gcgccccaaa ctgctcaccc cttaccgctt actgctcacc cctcacagag aaactacgaa
     3421 accattctga ccgaagcaca actggaaaaa tggttgaagc gtattcacgc ggcgcaattg
     3481 gtggcggtgg ataccgaaac caccggcctt gacccgatgc aggacaaact tgtagggata
     3541 tcgttttcag tagaggcaaa tcgagcggct tatattccgc tcacccaccg ttattccggc
     3601 gcgccggagc agcttgattc cgggcgggtt ttagagcggc tcaagccctg gctgaaagat
     3661 gcgggcaagc ccaaacttgg acagaatctc aaatacgaca aacacatttt cgccaatcac
     3721 ggcgcggcct tgaacggcat tgcgcacgat acgctgctcg aatcctatat cctggaaagc
     3781 cataagcccc acgacatgga tagtctggcg gagcgccatc tcggcgtgaa aaccattact
     3841 tatgatgaag tgacgggcaa gggcgcaaac cgcatcaatt tcgaccaggt ggcgattgat
     3901 cgcgctgccg aatatgccgc tgaagacgcc gacgtcactt tgcagctgca tcaggccttg
     3961 tatccccgga tcgccgcgga ttccaggctc gaagatattt accggcgcat cgagatgccg
     4021 cttctggaag tgctgttcag catggagaga aacggagtgt tgcttgacgt cgagctcttg
     4081 gacgagcaga gccgtgagct aggtagccgc atgctcgagc ttgaaaacca tgtgcaccag
     4141 gcagccggcc agccgttcaa tctcaattcg cccaagcaaa tccaggaaat tttattcgag
     4201 cgccagaagc tgccggtggt gaaaaaaacc ccaggcggcc agccttccac agatgaagac
     4261 gtgctgcagg agctggcgct gaattatccg ctgcccaagc tgatactcga ctatcgcggt
     4321 ttggccaagc tcaaatccac ttacaccgac aaactgccgc gcatggtcaa tccgcaaacc
     4381 ggccgcgtcc acaccaatta tgcgcaggcg gtggcggtga ccggcaggct cgccagcaac
     4441 gatccgaatt tgcagaatat cccggtgcgc acgcaggaag ggcggcgcat ccgcgaagcg
     4501 ttcatcgcgc cgcccgggca ccggattgtt tccgccgatt attcgcagat tgaacttcgc
     4561 atcatggcgc acatctcagg ggacaagagc ctgctcagag cttttgctga aggtcaggac
     4621 atccaccgcg ccacggcttc ggagattttc ggcatcccgc cggccgaggt cgggccggaa
     4681 caaaggcgtt atgccaaggt gataaatttc ggcctgattt acggcatgtc ggcgttcggt
     4741 ttagcgggcc agttgggcat cgagcgttcg gcggcgcagc tttacatgga gcgctatttc
     4801 gcgagatatc cgggcgtggc cgaatacatg cagcgcaccc gcgaacttgc caaacaacag
     4861 ggctttgtgg aaaccgtgct tggcagaagg ctttggcttc ccgacatcaa aagctcaaac
     4921 ggcacacgcc ggcagggggc agagcgcgcg gcgatcaacg cgccgatgca gggtactgct
     4981 gcggatctaa ttaaacttgc catgatcgca gtgcatgggt ggcttaagca aaagcggctc
     5041 aaagccaagc tcatcatgca agtgcacgac gagctggtgc tggaagtgcc ggatgacgaa
     5101 ctcgagttgc tgaaaaccga gttgccaaag ctcatgtgtg gcgtggccaa actgaatgtg
     5161 ccgttggtgg tcgatgtggg aaccgggccc aattgggaaa aagcacatta gtgtctgatg
     5221 acaagaagga gaaaaagtca cacaataatc accctcgagg aaatcgatga gagccatgaa
     5281 tacgcttaaa aacaggcgct tcctgatttt gctggcgctg ggcgcgctgt tggcggtgct
     5341 gttcacgcag gccgtgaccc aaaactcgcc cgcgccgcaa gtgacgttta cttcgctgaa
     5401 aggcgagaaa atcccgatga ggagcctgcg cggcaaggta gtgctggtga atttctgggc
     5461 caccagttgc gtcggctgcg tccacgaaat gccgcagatg gtagacacct accgcaagtt
     5521 ccaggcgcag gggctggaga ccatcgccgt ggcgatgagc tatgatccgc cgaattacgt
     5581 ggtgaactac actgagaaaa atgcgctgcc tttcaaagtg gcgctggatg tgcaagggga
     5641 cattgcccag gcatttggcg atgtgaagct cacgcccacc acttttgtca tcgacaagcg
     5701 cggcaacatc atcaaacgca tcctcggcga gcctgatttc gccaaactcc atcttttgct
     5761 ggaaaaagaa ttgcagaccg catgagacct cagcgaaacg agccgtgaca aaatataaag
     5821 gcaaaatccg cagttttgat ggggtttcgg catgatcggt tggaagcagc acttcatcta
     5881 tcaggttgac tatcagcact gggccaacga gcagatattc attatgctcg accgctttga
     5941 cgacaatgtg cgcaagagcc agcaaggcac gctgtccgaa agcatccaca acagcgtgga
     6001 ccacatgctg gtactcaccc gagtctggtt cggccgcctt aaggcacagc cgaacgaact
     6061 gcctttcaac caactgctcc atcccgattg gcgcgagctg aagcgcgcat tccgccagga
     6121 gctgcgcgat ttgcaacgct ggctggaaac caggccagaa gatttttttg cgcagcaggt
     6181 gagttacaag acgggtgagg gccgtgcggc ctcattctgg gtgcgcgatt tgcttactca
     6241 tttgatgctg caattgatcc atcaccgcgg ccaagtcacg tcggtggcgg gaaagctcgg
     6301 cgcgccggcg ccggaactgg gttatctgtt ttaccgccgc gagatggaca agtacctggc
     6361 gcaaatcagg cgctagatgg cggctatccc ggcttcatgc gcctgctggt cagcgtggta
     6421 gctcgagcgc accatgggtc cgcacgccgc gtgcaagaaa cccatttgcg aggccagttg
     6481 ttcaaactta ttaaataacg ccggctccgc atagcgtagc accggcagat ggtgcgcgga
     6541 gggttgaaga tactggccca gcgtcagcat atccacgtta tgctcacgca aatcgtgcat
     6601 cacttcgaga atttcctcat cggtttcgcc cagacccaac atcagtcccg acttggttgg
     6661 aatttcggga tggcgcgcct tgaattcttt taatagtctt agcgaattgt agtaatccgc
     6721 gccggggcgc gcctgcttat aaagccgcgg cacggtttcc aggttgtgat tcatcacatc
     6781 cggcggacag gcggaaagaa tgtccagcgc gatttccagt cttccgcgaa aatcaggcac
     6841 cagtatttcg attcgtatat gaggtgattt ttcgcgcacg cagcggatgc aatcggtgaa
     6901 atgccgcgcg ccgccgtcgc gcaaatcgtc gcggtccacg ctggtaatca ccacataatt
     6961 gagtttcaac acggagatgg tttgggcgag atgcatcggc tcgtccgcat cgggcggaag
     7021 cggtcttccg tgagccacgt cgcaaaacgg gcagcgccgc gtgcacaggt ctcccagaat
     7081 catgaaagtc gccgttccct tgccgaagca ttcgccgatg ttggggcagg acgcttcttc
     7141 gcagacggtg tgcaaattat gctcgcgcag ggtgcgcttg atttcgtaaa agcgcgtgcc
     7201 gcttgccgct ttcacgcgga tccagtccgg ctttttcagc cgcgccatgg gcacgatttt
     7261 gacgggtatg cgcgccgtct tggcttcgcc cttttgcttg cgctcttccg caaccgaact
     7321 catgttttct caagctgatg caggagtttt tgaataagtt tattgcccac ctgatcgggt
     7381 gagtcggcaa tgccgagcgc gcgcagttgc gtgacctgca agccgggatg accacagggg
     7441 ttgatggcgt gaaacggtgt cagatccatg tccacgttga gagccaggcc gtggtaacag
     7501 caaccgtttt tgattctcaa ccccagcgcg gcgattttcg cgccgctgac atacacaccc
     7561 ggcgcgtcca cccggttttg cgcttcgatg ccgtaatcgt gcagcaagtc tatcacggac
     7621 tgttccaagc ttctcaccag cgggcgcacg cctagtttca gcctgcgcat gtcgagcagc
     7681 ggataaatga caagctgccc cgggccgtga taggtaatct gtccgccgcg gtcggtttta
     7741 atgactggaa tggtggtgcg cttcggcaaa tgttcggact tgccgttcaa acccacggtg
     7801 taaaccggcg gatgctgcat cagccatatt tcatccggcg tattggcatc gcgctgtctc
     7861 gtaaaatcct gcatcgcctg ccaggcagtg agatattcaa ccgggcccaa gcgcctgatt
     7921 aagggtgaag cgtgaaacgt gaaacgtgaa acgggggaga gcagagtctc tctggtattg
     7981 ggcgtttcac ttttcacttt tcgcctttca cgtttcagag caccatgacc accatcgggt
     8041 gatcgcacaa ttcgcgatag aggtcgtcca gctgttcgcg ggacctggct tgaatgacac
     8101 aggtgaggct caggtatttg ccttttttgc tgggtttcat ttcgaggctc gcggggtcga
     8161 agtccggcgc atggcgcagc acgatttcca tcaccgcttg cgcaaaaccg gcctggcttc
     8221 tacccatgat tttgatggga aagtcacatg gatactcaat gagagaaggc tcgaggccca
     8281 ttttgattca gatggccggt gcttcggctt tgcgcatcac ggtttccttg taccgctggt
     8341 acaacccatg catgcgccag aagtgttccc ccggcttgcc gtcgcccacc ggtttgccgt
     8401 cgagtatggt aatggccagc acttcccggg tcgaagaagt catccataat tcctgcgcgc
     8461 tgcgcacttc gttttcggaa accaccctga cttcatgcgg gatattgttt gcagcggcga
     8521 gttccagcac cacgtcgtag gtgatgccag ccagcatcaa atgatttttc ggtggcgcaa
     8581 gcagcagatt gtctttcacc acaaggatat tgcttgccgc gccctcggtg agaaaaccgt
     8641 cacgcagcaa aacggcttcc gctgcgccgg catccgccgc catttgccta agcagcacat
     8701 tgggcagaag cgaaatcgcc ttgatgtcgc agcgcagcca gcgcttgtcc tgcgcggtaa
     8761 tagccgccac cccggttttc aaaagttccg gaggcggaat ggtgagcggc gtgctcatca
     8821 tgaacacggt ggggtgaacc tgcttgggga aagcgtggtc gcgctttgcc ggcccgcgcg
     8881 tgacatgcag gtaaaggtac tggtcttcgc cctggttttg tgcaatcagt tcaccgatga
     8941 gtcgggtcca ttcggcatcg ctgtgcgggt ttgcaaggcg aatggcgccg aggctcgtct
     9001 gcaggcgcag caagtgctgc ttgaggcgga atgggatgcg cgaataagcg ggaatgacct
     9061 catacacgcc gtcaccgaaa atgaagccgc ggtcgagcac cggaacccgc gcctcctccg
     9121 ggggcaggaa cttgccgttc aaataaacga tttgcctgct caattgaaaa gcaacctcag
     9181 agtatcccag ccgcggccga gaatattcgc caagccgata ctctccagcg caaccaccgg
     9241 gtactctgct atgggtttgc cgtccagggt tagtttgacc acgccgacat tctgtccggc
     9301 gctgatcggc gcgatcagcg gctgccggct ttccatggtg gccttcaggc gttccgcttg
     9361 ccccttgggc aggcttaaat agacatcatt ggaaaatccg gccttcactg aattctccgc
     9421 tcccttccag actgggaacg aagtgatcgc ctggcctttc aggtacagat gcagggtatc
     9481 gtaaaactgg aagccatagt tcaaaagctt ctggctctcg gtcgcgcgca cgctgtcgga
     9541 ggaggtgcca agcaccactg aaatcagccg ccgctcgccg cgcttcgcgg tggatatcag
     9601 gcaatagccc gcgttttcgg tatagccggt cttcataccg tccacgtaag ggtcggtcca
     9661 cagcagccgg ttgcggttgg attgcgagat gttgttatac ttgtattcct tggtcgaata
     9721 aagcggataa tattcgggaa agtcgcgaat gatggccgca gcaaccaggg aaaggtcatg
     9781 cgcggtggaa taatgctgcg ggtgcggcaa gccggttgag ttcatgaagt gcgtgtcctt
     9841 catgcccagg cgctgcgctt ctcggttcat catctgtgcg aacacctcct cgctgccggc
     9901 aatgccttcg gcgagcgcaa tgcaggcgtc gttgccggat tgcacaatca taccgtgtat
     9961 cagctcatcc accgtcaccg gccggttcgg ctcaatgaac atgcgcgagc cctcggcgcg
    10021 ccaggcacgg gtggagaccg gcagggtctg gttcagcgtg atctgcttct gcttgagcgc
    10081 agtaaaagtg agataggcgg tcatgagttt ggtgagcgaa gccggctcga tgcgctcgtt
    10141 gacgttttgc ctcaccagca cctgcctgct gtaaaaatca gttagcagat aggacttcgc
    10201 ggcgacggga ggcgcctgcg gtacaggcag ttg
//
LOCUS       DATIOW010000023         2518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1330056, whole genome shotgun sequence.
ACCESSION   DATIOW010000023 DATIOW010000000
VERSION     DATIOW010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330056"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VLL03_00645"
     CDS             complement(<1..142)
                     /locus_tag="VLL03_00645"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HSS45913.1"
                     /translation="MNKACEMTKQYAPLVGRALLSLIFILSGWGKITGFSGTAAYMAN
                     KGM"
     gene            complement(170..868)
                     /locus_tag="VLL03_00650"
     CDS             complement(170..868)
                     /locus_tag="VLL03_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HSS45914.1"
                     /translation="MIVLRKSADRGYFDHGWLKTYHTFSFADYFDREHMNFGSLRVIN
                     EDFVQAGKGFGTHPHRDMEIITYILEGQLAHKDSMGNGSIIKRGDVQRMSAGTGVTHS
                     EYNASQTEAVHLLQIWILPKLNGIKPGYEQKYFSDAEKTGKLRLVVSPDGRDDSVTIH
                     QDAFMYAGLLSQGKELSHSPASGRRAYLQVARGALRVNGQRLEAGDGVKVTDEKTITL
                     AASENSELLLFDLA"
     gene            1069..1344
                     /locus_tag="VLL03_00655"
     CDS             1069..1344
                     /locus_tag="VLL03_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS45915.1"
                     /translation="MLSKRVVSGLALGAAMLISAAAPVVTQAHDRVYIDSGYTVYPGN
                     SYYGYHRYCQPRAYFPYYSSNSYYYGSGYGYSHPAYGNRLTIIYRSY"
     gene            1384..1911
                     /locus_tag="VLL03_00660"
     CDS             1384..1911
                     /locus_tag="VLL03_00660"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HSS45916.1"
                     /translation="MNKRIPLIAALALASASATAFANDYEDIAIVKSASPQYEQVNVP
                     RNQCYTEYVPAPRYQSSNGYAGPLIGGLAGALLGNTVGRGTGNKAATAGGAVIGAIVG
                     DRISRNADYGGDSYHEVRRCNVVDSYESRLAGYHVVYEYAGRTYTALLPYDPGRELHV
                     RVNVDPVGGPAHYNR"
     gene            2061..2240
                     /locus_tag="VLL03_00665"
     CDS             2061..2240
                     /locus_tag="VLL03_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS45917.1"
                     /translation="MVAQRGRISAGEAAQIVQRRTGARVLDAQPARDGYRVKVLTRQG
                     EVRVYFVDAQTGAMQ"
     gene            2255..>2518
                     /locus_tag="VLL03_00670"
     CDS             2255..>2518
                     /locus_tag="VLL03_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS45918.1"
                     /translation="MRVLVIEDELLLQKQIATRLGAEGYVVDVSSDGKDGFYRAAEYP
                     HDVLIVDLGLPGLSGIEIIRKLRTQGKLVPILILTARGRWQDKV"
BASE COUNT          527 a          737 c          696 g          558 t
ORIGIN      
        1 gcatgccctt gttcgccata taagcggcgg tgccggaaaa accggtgatc ttgccccagc
       61 cggatagaat aaaaattaac gacagcagcg cccggccgac cagcggcgca tactgcttgg
      121 tcatttcgca tgctttgttc atgatcgcac ccctttctag ttaatacact caagcaaggt
      181 caaacaacaa taattcgctg ttttccgaag cggcaagcgt tatggttttt tcgtcagtga
      241 ctttcacgcc atcccccgct tccagccgct ggccgttcac cctgagcgcg ccgcgcgcca
      301 cttgaaggta agcgcgccgc ccgctggccg gcgagtggct caattccttg ccctggctca
      361 aaagccccgc atacatgaag gcatcctgat gtatcgtcac cgagtcatcg cggccatcgg
      421 gcgaaaccac cagccgcagt ttcccggttt tctccgcatc gctgaagtat ttctgctcgt
      481 agcccggctt gatcccgttt aattttggca gaatccagat ttgtaatagg tgcaccgcct
      541 ctgtttgcga agcattgtat tcgctgtgcg tcacgccggt gcccgcgctc atacgctgca
      601 catcgccgcg cttgatgatg gagccgttgc ccatgctgtc cttgtgcgca agctgtcctt
      661 ccagaatata agtgatgatt tccatgtcgc ggtgcgggtg cgtgccgaag ccttttcctg
      721 cttgaacaaa atcttcgttg atgacgcgca agctgccaaa gttcatatgc tcgcggtcga
      781 aataatcggc gaatgaaaag gtatggtagg tcttgagcca gccgtgatcg aaatagccgc
      841 gatccgcgct ttttcttaaa acaatcatga caaatccttg agaaattcac tggcggggtc
      901 gtttttgacc ccacttgtcc agcattaagt cattgatagg caattatatt cctaaaccgc
      961 cgggatttca ccttccttcg agcattccgt tcagctttcg ttcagcatgc cccgcgtagg
     1021 atgcgagtca tcaggcaagg cctgagactt tatgaaagga ggtttcaaat gttaagcaaa
     1081 cgtgttgttt cgggattggc gctgggagcg gcaatgctaa tcagcgccgc ggcgccagtg
     1141 gtgacgcaag cgcacgaccg cgtttacatc gacagcggtt acacggtgta tccggggaac
     1201 agctactacg gctatcaccg ctattgccag ccgcgtgcgt attttccgta ctactccagc
     1261 aatagttatt actacgggag cggatatggc tacagccatc cagcttatgg caatcgccta
     1321 accatcattt atcgcagtta ttgatttgat taactcttaa ctatttactg aaaggagcac
     1381 aaaatgaaca aacgcattcc tctgatcgca gccttggcac tggcgtccgc ttctgcgacc
     1441 gcattcgcca acgactacga agatatcgcc atcgtcaaaa gcgcgagccc gcagtacgag
     1501 caagtcaacg tgccgcgcaa ccagtgctac accgagtatg ttccggcgcc gcgctatcag
     1561 agcagtaatg gctatgccgg cccgctgatc ggcggtctgg ccggcgctct acttgggaac
     1621 accgtgggcc gcggtaccgg caacaaggcg gcgaccgccg gtggtgcggt gattggcgcc
     1681 attgtcggcg accgcatctc gcgcaacgcc gattacggcg gcgattccta ccatgaagtg
     1741 cgccgctgca atgtggtgga cagctacgaa tcccgccttg ccggttatca tgttgtatat
     1801 gaatacgccg gccgcacgta caccgccctg ctgccgtatg atcccgggcg cgaattgcac
     1861 gtgcgtgtta acgtggatcc ggtgggtggt cccgcgcact ataatcgcta accggccggc
     1921 ggcttgactt tggcgcacgc gcccaccaag atggcggact tatgaaaaac ctgatctact
     1981 cattcctcct cctcgccttg tgtgcggcag agcctgttac cgccacacac gggcgggata
     2041 cgggtgactg ggatacatcc ttggtggcac agcggggccg catctcggca ggcgaagcgg
     2101 cgcaaatcgt gcagcgtcga accggcgcgc gcgtgctcga cgcccagccg gcgcgagacg
     2161 gctaccgcgt caaggtgctc acgcgccagg gtgaggtgcg ggtgtacttt gtggatgccc
     2221 aaaccggagc tatgcagtag gaacctcttc ggtaatgcgc gtcctggtca tcgaagacga
     2281 gctgctgctg caaaaacaaa ttgcgacacg cctgggggcg gaaggttatg tggtcgacgt
     2341 cagcagcgac ggcaaggacg gattctaccg cgccgccgaa tatccgcacg acgtcctcat
     2401 cgtcgatctt ggcctaccgg ggctttccgg cattgaaatc atccgcaagc tgcgcacgca
     2461 gggcaaactc gtgccgatcc tgattctcac ggcacgcggc cgctggcagg acaaggtg
//
LOCUS       DATIOW010000024         9263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_720741, whole genome shotgun sequence.
ACCESSION   DATIOW010000024 DATIOW010000000
VERSION     DATIOW010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9263
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720741"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VLL03_00675"
     CDS             <1..544
                     /locus_tag="VLL03_00675"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HSS45919.1"
                     /translation="ILDRAQGDIHPAGNPHILLDPRNIAKVGAALTERLSQLDPGNAD
                     AYQVRGKAFQDRWQTAIQRWEQEGAPLKGVPVITHHKDMTYLIHWLGMREVGNLEPKP
                     GIPPTAGHLAELFANMQRDPAKMILRTPFQDARASESLGSRAKIPAIVLPYTVGGTDK
                     AKDLFGLFDDTLQRLLQAEK"
     gene            541..1320
                     /locus_tag="VLL03_00680"
     CDS             541..1320
                     /locus_tag="VLL03_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HSS45920.1"
                     /translation="MNLDALTLGILAPAFLAGLLVLATHVPLGIQVLKRGIVFIDLAI
                     AQIAGMGVIAADFMGFEPQGWAVQLAALSAALAGALILTWTERRWPEVQEALIGSLFV
                     LAATGGILLLANNPHGGEHLKDLLVGQILWVSFAQLIPVAVLTAVICILWFRFQQKLG
                     HTGFYLLFAVSVTASVQLVGIYLVFASLIIPALAARNYARLRLTAGYALGAAGYALGL
                     VLSALFDLPSGAVIVWALATLGIVFYLLQAETTQTKDATQL"
     gene            1429..2550
                     /locus_tag="VLL03_00685"
     CDS             1429..2550
                     /locus_tag="VLL03_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSS45921.1"
                     /translation="MVFPFFRNKDQEEGRSDRASAAKKDEAGSRAAKTEFGTGTEMEV
                     LDRTSELSAAAEEAAMLYANNLTELTYELLLTTVKAGAPDLEAWLMLFDLYQQKGMQK
                     EFEELALNFVVKFERSPPAWREIKATNTTLAQQDGTFALSGELNAASGSQFDEMARIA
                     ENTDQLRLDFSEIESVAPTGCVLLLQTLRNIRKGGHRIAITGFGHLVQMMQSATNPVP
                     EPEQLILLLELYQVKGMQKEFEDLALQYAMKFEVSPPSWEPLPQEQAQVGKESPEPAP
                     AELEANQEGYAMHGEVVGSADPQLQGISGYAAQRQDVNIDMSDVRRMDFVSCGMFLNI
                     LAGLKQSGKNITITGANEMILSLWRVMEVHKVATLIRKK"
     gene            2562..2939
                     /locus_tag="VLL03_00690"
     CDS             2562..2939
                     /locus_tag="VLL03_00690"
                     /inference="COORDINATES: protein motif:HMM:NF022610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HSS45922.1"
                     /translation="MPRPTRQLVRRIVLFALVFSAISPALAALRFYGNAEVLANIASV
                     YTAAHNSAEHQHSGHANSKVHQVYCSFCLDMASVQALTLAAPHLPLSAMAAEPPPRAV
                     PHSAERVRCYSPQHSRAPPIPSV"
     gene            3031..3969
                     /locus_tag="VLL03_00695"
     CDS             3031..3969
                     /locus_tag="VLL03_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS45923.1"
                     /translation="MPVLKRGFLFVTTFFLLAATEAWAHGFAGKRFFPATLAVDDPFI
                     SDELTFLTNHIREPDQVTNEFSVAYSKRITPNFGFELGETYQHLKPNEGGSKNGFANL
                     KVGAKYQFFTSEVHEAILSVGLDAELGRTGSRNVGTESFSTLSPTFFFGKGFGDLPES
                     LKYLKPVAITGAIGPSFPTRKTNVTTTVDPVTGNINQDIERNPTSLNWGFSFQYNLQY
                     LQSFVKDVELAEPFNRMIPVVELALSTCLNADCKGQTTGTVNPGIIWFGKYAQLGLEA
                     QIPVNQRTGNHVGVLFQMHFFIDDLFPQSVGRPIFR"
     gene            3966..4340
                     /locus_tag="VLL03_00700"
     CDS             3966..4340
                     /locus_tag="VLL03_00700"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HSS45924.1"
                     /translation="MKKFRIKLIIALCGLALAAASPRWAGAHAFPDHAEPRVGAVISI
                     SPTRVRIWFDGVLEPLFSTLQVRNTSGQPVDRGDGHVDASDQTLLEISLPHLPPDTYR
                     VFWSVVARDGHRTQGDYTFTLR"
     gene            4353..5408
                     /locus_tag="VLL03_00705"
     CDS             4353..5408
                     /locus_tag="VLL03_00705"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HSS45925.1"
                     /translation="MWSNLVHSFPNALDLLALTTCIGTLACRLWAVPATPVESSVIQP
                     LLARLWRLLLVCLTVLVLSSAIELIQRAAEMSGRPLGEVFSILHAVLFNSHYGQAWLV
                     RVIALVMLWVGWLLGRRKLDSRTVPIFMLISAVLIALTRSASGHGADAGDMSLPELMD
                     WFHLLAASLWSGSLVAFTTVIFPTWFRYGGQHAQLIAHMARRFSALAGIALAAILLTG
                     LYNTWFEVRTLSALWTTSYGNILSIKLLLVLALIILGASNRYISVPLLERRAGYPQTG
                     NGLFKWFTLRYLSHPQGNMDETSVARHFAHKVMTEVILMVSVLVCAALLIHETPARHQ
                     PHTGHMTLQPHFGHGTH"
     gene            complement(5450..6295)
                     /locus_tag="VLL03_00710"
     CDS             complement(5450..6295)
                     /locus_tag="VLL03_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HSS45926.1"
                     /translation="MCVFVLALALAACGSTPKRQPGKGYYLDDGPGDNPPSNLDTLPD
                     AQPRVEPLNQYANRPYTVLGKNYVPETRFVPYKARGTASWYGRKFQGQRTSSGEPYNM
                     YAMTAAHPILPIPSYARVTNLRNNKSVVVRINDRGPFYSGRIIDLSYAAAYKLGIAAQ
                     GSGTVEVESVQPGTETLVAQAPKPAVPDPAPPPVPAQVLPAAADSSGIYLQLGAFSSQ
                     GNAEQFLTRLRSQLASFSETLSIFTKDGLFRVHAGPYANRSEAQHAADKIGEALGFKP
                     LLLIR"
     gene            complement(6374..7468)
                     /gene="rodA"
                     /locus_tag="VLL03_00715"
     CDS             complement(6374..7468)
                     /gene="rodA"
                     /locus_tag="VLL03_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HSS45927.1"
                     /translation="MIKRLWHHLTAHIDGFLLATVCVLMLLGLFVLYSATGQNLSRVS
                     NQLVSMLIALALMWAIAKMPPHYLMRLSLPTYVIGMVLLICVALFGDVVNGARRWLHV
                     GVARIQPSELMKIAVPLMLAWYFDKHETALKLQNYVVAILSLLVPIVLIARQPDLGTA
                     ILITASGFYVLFLAGLSWRIMLGFFALGLASLPVLWSVMHDYQRQRILTLFDPSTDPL
                     GAGYHTIQSNIALGSGGVTGKGWLNGTQSHLEFLPEHHTDFIFAVFGEEFGLLGNLLL
                     LFLYALVIGRGLVIASNASTFFTRLMAGAITLTFFTYAFVNMGMVSGILPVVGVPLPL
                     LSYGGTSLVTICLGFGILMSIQAHQKLVKT"
     gene            complement(7461..>9263)
                     /gene="mrdA"
                     /locus_tag="VLL03_00720"
     CDS             complement(7461..>9263)
                     /gene="mrdA"
                     /locus_tag="VLL03_00720"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HSS45928.1"
                     /translation="LLIARFVYLQVVQNEYYQTLAESNRISVVPIVPNRGLILDRNGA
                     VLAHNYSAYTLEITPSRVDNLEALINQLATLVEITPKDRKRFRKLLEESKNFESLPIR
                     TRLNDVEVARFAVNRYRFPGVEIKARLFRHYPLGEMASHVIGYINRINDDDLEWLEEQ
                     GALPNYRGSDYIGKTGIEQSYEMELHGRAGFEQVEIDAGGRAVRTLDRTPPISGNNLL
                     LSLDARLQEFAVKAFGNFRGALVAIEPASGGILALVSKPGFDPNLFVDGIDPQNWDAL
                     NGSPDKPLTNRAMHGQYPPGSTFKPFMALAGLELNKRSPQYTIYDPGYFTLPGSTHRY
                     RDWKAGGHGIVDLHKSLVISCDSYYYGLANELGIDNIHSFISQFGLGSKTGIDIEGEA
                     AGILPSQEWKQNRYREKWFAGDTVSVGIGQGYNLVTPLQLANATAILANNGIAFRPHL
                     VRNIQDGKTELLRGVSNKVFYALNLKQSNLELVKKALIDVMLPGGTAARAGAGSLYPI
                     AGKTGTAQVIALKQNEKYDESKVAERQRDHALFIAYAPADRPQIALAVLVENGGHGAS
                     TAAPLARQVLDFYLLGKRPQSPLPRLDEKDEAND"
BASE COUNT         1895 a         2642 c         2688 g         2038 t
ORIGIN      
        1 gatactggat cgtgcccagg gagacataca tcccgctggg aatccgcata ttcttctcga
       61 cccgcgcaac attgccaaag tgggagcagc gcttaccgag cgcctgtcgc aactggaccc
      121 cggcaacgcg gatgcttatc aggtccgtgg taaagcgttc caggatcgct ggcaaactgc
      181 gattcagcgc tgggagcagg aaggagcacc actcaaagga gtaccggtca ttacccacca
      241 caaagacatg acttatctga tccactggct cggcatgcgc gaagttggca atttggagcc
      301 taaacccggg attccaccca ctgcgggaca ccttgcggag cttttcgcca atatgcaacg
      361 agacccggcg aaaatgatac tgcgtacgcc gttccaggac gcccgggctt ccgagtcgct
      421 gggcagccgc gccaagattc cagccatcgt attgccttac accgtaggcg gcaccgacaa
      481 ggcaaaagac ttattcggct tgttcgatga tactttgcaa cgtttattgc aggcggaaaa
      541 atgaatctcg atgcgctgac tctaggcata ctcgcgccgg ccttccttgc cggcttgctg
      601 gtgcttgcca cccatgtgcc gcttgggatt caggttttga agcgcggcat cgtgttcatc
      661 gatctggcga ttgcgcaaat tgcggggatg ggggtgattg ccgcggattt catgggtttt
      721 gagccgcaag gttgggcggt gcagcttgcc gcactgagcg cggcgttggc tggagcactg
      781 atacttacct ggactgagcg gcgctggccg gaagtgcagg aagcgttgat cggatccctg
      841 ttcgtactgg cggcgaccgg cggaattttg ctgctcgcca acaatccgca tggcggggag
      901 catctgaagg atttgctggt cggccagatt ctatgggtga gcttcgcgca actgattccg
      961 gtggcggtat tgaccgccgt gatttgcatc ctgtggttca ggttccaaca gaaactcggg
     1021 cacaccggat tctatctgct gtttgcggtt tcagtcacag cttcggtgca gttggtgggc
     1081 atttatctgg tgtttgccag cctgattatt cctgctttgg ccgcacgcaa ttacgcgcgc
     1141 ctgcgcctga ctgcggggta tgcgcttggc gcggcgggtt acgccctcgg tctcgtatta
     1201 tccgccctgt tcgatttgcc ctcgggagcg gtgatcgttt gggcgctcgc cacactgggc
     1261 atcgtgtttt atttgttgca ggcagaaaca acccagacta aagacgcgac ccagctctga
     1321 ttttccgatc taattgcggg gatccggttg gtgttataaa attcaactgc agtaccgccc
     1381 gtccaacaca tccccattat cacggaatca ctagaactaa ggtggcaagt ggtttttcca
     1441 ttttttcgca ataaagatca ggaggagggc aggtctgatc gcgcctcggc cgcgaagaag
     1501 gacgaggccg gctctcgagc cgctaaaacc gagttcggca ccggcactga aatggaagtc
     1561 ctggatagga ccagcgagct ttccgctgcg gctgaagaag cggctatgct ctatgcgaac
     1621 aacctgaccg agctgactta cgagttgctg ttaaccacag tcaaggcggg cgcgcccgac
     1681 ctcgaagctt ggctgatgct gttcgatctt taccagcaaa agggaatgca gaaggaattc
     1741 gaggagttgg cgctaaattt tgtggtcaag ttcgaacgct cgccgcccgc ctggcgcgaa
     1801 atcaaggcaa caaacaccac gctcgcccag caagacggca cctttgcctt gtccggggag
     1861 ttgaacgcgg cttccgggtc gcagttcgac gagatggcca ggattgccga aaacaccgac
     1921 cagttgcgcc tggatttcag cgaaattgaa agcgtcgcac ccacagggtg cgtgctgctg
     1981 ctgcaaactt tgcgcaacat ccgcaaaggt gggcaccgca ttgcgattac cggctttggc
     2041 catctggtgc aaatgatgca aagcgcaacc aacccggttc ccgaaccaga gcagctaata
     2101 ctgttgctgg aactgtatca agtgaaaggc atgcagaagg agttcgagga cctggctttg
     2161 cagtacgcga tgaagtttga agtctcgccg ccctcgtggg aacccttgcc acaagagcaa
     2221 gcgcaggtag gaaaagagag cccagagccc gcgccggcag aactcgaagc caaccaggag
     2281 ggctatgcca tgcacggcga agtagtcggt tccgccgacc cacaattgca aggcatttcg
     2341 ggatatgccg cgcagcggca ggatgtgaac atcgacatgt cggatgtgcg ccgcatggat
     2401 tttgtcagct gcggaatgtt cctcaacatc ctcgccggtt tgaagcagag cggcaaaaac
     2461 attactatta ccggtgccaa cgaaatgatc ctctccctgt ggcgggtgat ggaagtgcac
     2521 aaagtggcga ccctcattcg aaaaaaataa accaccagac catgcctcgc ccaacccgcc
     2581 aacttgtccg ccgtattgtg ctgttcgcgc tggtgttcag tgcgatttca cccgcgcttg
     2641 cggcgttgcg cttctacggc aacgcggaag ttttggcgaa tatcgccagc gtttacacgg
     2701 cggcgcacaa ctcggccgag caccagcatt ccggtcacgc aaactccaag gtgcaccagg
     2761 tttactgctc gttttgtctg gacatggcat cggttcaggc actgacactg gccgcgccgc
     2821 atttgccgct ctccgcaatg gcggcggaac caccgccccg cgctgttccg cacagcgccg
     2881 agcgagtgcg ctgttactcc ccgcaacatt cccgagctcc tcccatcccc tccgtctgat
     2941 cccgcgggcg cttaagcccg aaaatgcgcc aaatcctcgc accttgggct tggcgccgtt
     3001 ccatattcta ttcgtttagg gagaaacgag atgccggtat tgaagcgggg gtttttgttc
     3061 gtgacgactt ttttcttgct tgccgcgacc gaagcttggg ctcatggttt cgccggcaag
     3121 cgattctttc ccgccacgct ggcggtggac gatcctttta tttccgacga gctgacattt
     3181 ctgaccaatc acattaggga acctgaccag gtgaccaatg agttttcggt ggcttactca
     3241 aagcgcatca cgccaaactt cgggttcgag ctgggcgaga cgtatcaaca cctgaagccg
     3301 aatgaaggag gaagcaaaaa cggctttgca aatcttaagg tgggcgccaa atatcagttt
     3361 tttacaagcg aggttcacga agccatcctc tccgtcgggc tcgacgccga actcggccgc
     3421 acgggcagcc gcaacgtggg caccgagtcg ttttcgacgc tttccccgac cttctttttt
     3481 ggcaagggat tcggtgacct ccccgaatcg ctgaagtacc tcaagcctgt tgcaatcacc
     3541 ggtgctattg gccccagctt tccgacgcgc aagaccaatg tgacaaccac cgtggatccc
     3601 gttaccggaa atatcaacca ggacatcgag cgtaatccga caagcttaaa ctgggggttt
     3661 tccttccaat acaatcttca gtatctgcag tcattcgtaa aagatgtgga gttagcggaa
     3721 ccgtttaacc ggatgattcc cgtggtcgag cttgccctca gtacttgcct gaatgcggac
     3781 tgcaaagggc agaccaccgg tacggtgaat ccgggcatca tctggtttgg caaatacgct
     3841 cagctcgggc tcgaggccca gattcccgtc aaccaaagaa cggggaacca tgtcggtgta
     3901 ttgttccaga tgcatttctt cattgacgat ctgttcccgc aaagcgtggg gcggccgatt
     3961 ttccgttgaa gaagtttaga atcaagctca ttatcgcttt gtgcggcctt gcgctcgccg
     4021 ccgcatcgcc gcggtgggcg ggggcgcacg cctttcccga ccatgccgaa ccgcgggtgg
     4081 gagcggttat cagtatttct cccacccgtg tacgcatctg gtttgacggc gtcttggaac
     4141 cgctcttcag cacgctgcaa gtccgcaaca cgagcggcca gccggtggac cgaggtgacg
     4201 gacatgtgga tgcgtcggac cagacgctgc ttgaaatcag cttgccccat cttccacctg
     4261 acacttaccg cgtcttctgg agcgtcgtcg cccgcgatgg acaccgtacc caaggtgact
     4321 acacgttcac tctgcgttaa gggcatttga ttatgtggag caatcttgta cacagttttc
     4381 cgaacgcact cgacctcttg gcactcacca cgtgcattgg gactttggcc tgccgcctct
     4441 gggcggtccc tgccacgccg gtagagtcgt ctgtaattca gcctctattg gcgaggctat
     4501 ggcggttgct tcttgtctgt ctcaccgttc tcgtgctgag cagcgccatc gagctgatac
     4561 agcgtgcggc cgaaatgagc ggtcgtccgc tcggtgaagt gttttcgata ctgcatgcgg
     4621 tgctcttcaa cagccattac gggcaggctt ggctggttcg tgttatcgcc ctggtgatgt
     4681 tatgggttgg ctggttgcta ggcagacgaa aattggattc gcgcactgtt cctatattca
     4741 tgctgattag cgcggtactc atcgctttga cgcggagcgc ctctgggcac ggtgctgatg
     4801 cgggggatat gagtcttccg gaactcatgg attggtttca tctcctcgcg gcctcgctat
     4861 ggagcggtag tttggttgcg ttcactaccg ttattttccc cacctggttc cgctatggcg
     4921 ggcagcacgc ccagctgatt gcccacatgg cgcgacggtt ttccgctctt gccggcattg
     4981 ccctcgccgc cattttgctg accggactgt acaatacctg gtttgaagtc agaactcttt
     5041 cggcgctttg gacgacgtct tacggtaaca ttctcagcat caagcttttg ctcgtgctgg
     5101 cgcttatcat tctgggcgct tccaatcgct acattagcgt accgctgctt gagcgccggg
     5161 ctggctatcc tcaaacaggc aatggcttgt tcaagtggtt tactctgcgt tatctttctc
     5221 accctcaggg caacatggat gagaccagtg ttgcgcgtca cttcgcgcat aaggtgatga
     5281 cagaggtaat ccttatggta agcgtgctgg tttgcgcggc gttacttatt catgaaaccc
     5341 cggccagaca ccaaccccac accgggcata tgactctcca gccacacttt ggtcacggaa
     5401 cgcactagtt cccaaccttt gaactggctc atgccgtttt aacaattgcc taacggatta
     5461 acagcaacgg cttgaagccg agcgcctcgc cgatcttgtc ggcggcatgc tgtgcctcgc
     5521 tgcggttggc ataagggcct gcgtgtaccc ggaacaaccc gtccttggtg aaaatcgaaa
     5581 gtgtttcgct aaagctggca agctgcgagc gcagccgcgt cagaaactgt tccgcgttgc
     5641 cctgtgagct gaacgcgccg agctgcaagt agatgccact gctatccgcc gccgccggca
     5701 gcacctgggc gggaacgggt ggtggtgcag ggtcgggcac ggctggcttt ggtgcttgcg
     5761 ccaccagcgt ttcggtgccg ggctgcacgc tttccacctc aaccgtgccg ctgccttgag
     5821 cagcgatgcc tagcttgtac gcagcggcat aagagagatc tataatgcgc ccgctataga
     5881 acggcccgcg gtcattgatg cgcacgacaa ctgatttgtt gtttctaagg ttggtgaccc
     5941 gggcatagct gggtatcggc aaaatggggt gcgctgcggt catggcatac atgttgtaag
     6001 gctctcctga tgatgttctc tgtccttgga attttctgcc ataccaggaa gcggtgccgc
     6061 gcgccttgta aggaacgaag cgggtttcag gaacatagtt tttgccgaga acagtgtacg
     6121 gccggtttgc gtattggttg agcggctcaa cgcgcggttg agcatcgggt agcgtatcaa
     6181 ggttcgacgg cggattatcg cccgggccat cgtcgagata ataacctttg ccaggttgtc
     6241 gcttgggcgt gctgccgcag gcggcgagag ccagcgccag gacgaaaaca cacaagaagc
     6301 gtaaagtaga gttaatgtgt ttgggccttt gtttccactc ttctctcttc ttcacccctc
     6361 atacctcgca ggctcaagtt ttaaccaatt tctggtgcgc ctggatgctc atcaaaatac
     6421 caaagcccag gcagattgtc accagcgaag tgccgccgta gctcagcagc ggcagcggca
     6481 cgccgaccac cggcaagatg ccgcttacca tgcccatgtt gacgaaggcg taggtaaaaa
     6541 aagtgagtgt gattgccccc gccatcaatc gcgtaaagaa ggtcgaggca ttgctggcaa
     6601 taaccagtcc cctgccgatc accaatgcgt ataggaacag cagcaacagg ttgccgagca
     6661 ggccaaattc ctcgccgaac acggcgaaaa tgaaatcagt gtggtgttcc ggcaggaact
     6721 ccaggtgcga ctgcgtgccg ttcagccagc ctttgccggt tacgccgccc gaaccgagcg
     6781 caatgttgga ttgaatggtg tgatagcccg cgcccagcgg atcggtggaa ggatcgaaca
     6841 aagtgagaat gcgctgccgc tggtagtcgt gcatcactga ccacagcacc ggcaggctcg
     6901 ccagccccag cgcaaaaaag ccgagcatga tgcgccagct caagcccgcg agaaacagca
     6961 cataaaaacc ggaagcggtg ataagtatgg cggtgccgag atctggctgg cgcgcaatca
     7021 gcactatcgg cactagcagc gacaagatcg ccaccacgta attctggagt ttcagcgccg
     7081 tctcgtgctt gtcgaaatac caagcgagca tcaacggcac cgcgattttc atcagctcgg
     7141 aaggctggat gcgggccacg cccacatgca gccagcgccg cgcgccgttc accacatcgc
     7201 cgaacagcgc cacgcaaatc aggagcacca tgcctataac atacgtcggc agagacaggc
     7261 gcatcaggta atgtggcggc atcttggcaa tcgcccacat cagcgcgagc gcgatcagca
     7321 tgctgacaag ctggttgctg acgcggctta agttttgccc ggtggcgcta tagagcacga
     7381 acagtccgag cagcatcagc acgcacaccg tagcgaggag aaaaccgtca atgtgcgcgg
     7441 tgagatgatg ccacaagcgt ttaatcattg gcttcgtcct tttcgtccag tctgggcaga
     7501 gggctttgcg ggcgtttgcc gagcagataa aaatccagca cttggcgggc gagcggcgcc
     7561 gcagtggagg cgccgtggcc gccattttcc accagcaccg ccagcgcaat ctgcggccgg
     7621 tccgccggcg cgtaggcgat gaacagcgca tgatcgcgct gacgctccgc caccttgctc
     7681 tcatcgtatt tttcgttttg ctttaaggcg atgacctgcg cagtcccggt cttgccggca
     7741 atcgggtaca agcttccggc gccggcgcgc gccgcagtgc cgccgggaag catgacgtcg
     7801 atcagcgctt ttttcaccag ttccagattc gactgtttga ggttgagtgc atagaacact
     7861 ttattggaaa cgccgcgcaa cagctcggtc ttgccgtcct gtatgttgcg caccagatgc
     7921 gggcggaagg caataccgtt gttggcgaga attgcggtgg cgttggcgag ctgcaggggc
     7981 gtcaccaagt tgtagccctg gccgatgccg acggacacgg tgtcgcccgc gaaccatttt
     8041 tcccggtagc gattttgctt ccattcctgc gacggtagaa tgccggccgc ttcgccttcg
     8101 atgtcgatgc cggtcttgct gcccaggccg aactggctga tgaaggaatg aatgttgtcg
     8161 atgcccaatt cgttggcgag gccatagtaa taactgtcgc acgaaatcac cagtgacttg
     8221 tgcaaatcga cgatgccatg gccgccggct ttccagtcgc ggtagcggtg ggtactgccg
     8281 ggaagagtga aatagcccgg atcatagatg gtgtattgcg gcgagcgctt gttgagctca
     8341 agtccagcca gcgccatgaa cggcttgaag gtcgagccgg gcggatattg cccgtgcatc
     8401 gcgcgattgg tgagcggctt gtccggggaa ccgttgagcg catcccagtt ttgcggatca
     8461 atgccgtcga cgaagaggtt cggatcaaaa cccggcttgc tgaccaaggc gaggatcccg
     8521 ccgctcgcgg gctcaatcgc caccagcgcg ccccgaaagt tgccgaaagc tttcaccgca
     8581 aactcctgca gccgcgcatc cagcgaaagc agcagattgt ttccggaaat cggcggcgtg
     8641 cgatcgagcg tgcgcacggc ccgcccgccg gcatcgattt ccacctgctc gaacccggcc
     8701 cggccgtgca attccatttc gtagctttgc tcgatgccgg tcttgccgat gtaatccgag
     8761 ccgcggtaat tgggaagcgc gccctgctcc tccagccact cgaggtcgtc gtcgttgatg
     8821 cggttgatgt agccgatgac gtgcgaggcc atttcgccga gcggataatg ccggaacagg
     8881 cgcgccttga tttccacgcc ggggaagcgg tagcggttca ctgcgaagcg cgcaacctcc
     8941 acgtcattca ggcgggtgcg gatcggcagg ctttcgaaat tcttgctttc ctcaagaagc
     9001 ttcctgaaac gcttgcggtc cttcggcgtg atttccacca gcgtggcgag ctgattgatg
     9061 agcgcttcga ggttgtccac cctggacggc gtgatttcca atgtgtaggc ggaataattg
     9121 tgcgccagca ccgcgccgtt gcggtcgagg atcagcccgc ggttgggcac gatcggcacc
     9181 accgagatgc gattgctctc ggcgagggtc tggtaatact cgttctgcac gacctgcagg
     9241 tagacgaagc gcgcgatcag cag
//
LOCUS       DATIOW010000025         4042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_213524, whole genome shotgun sequence.
ACCESSION   DATIOW010000025 DATIOW010000000
VERSION     DATIOW010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213524"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /gene="glnA"
                     /locus_tag="VLL03_00725"
     CDS             complement(<1..1277)
                     /gene="glnA"
                     /locus_tag="VLL03_00725"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HSS45929.1"
                     /translation="MASPADVFKIIKEKEVKFVDLRFTDTRGKEQHVTVPVKAFSELK
                     FTDGHFFDGSSIAGWKSIEASDMVLLPDPDSARMDPFTDEAVLNITCDVIEPSDGKGY
                     DRDPRSIAKRGEAYLKSTGIADTAFFGPEPEFFIFDSVTWNVDMSGCFVKIKSEEAPW
                     SSGEEFEGGNSAHRAPVKGGYFPVPPTDSLHDIRNAMVLAMEEQGVVCEVHHHEVAAA
                     GQNEIGTLYNSLVKRADWMQILKYTIWNVAHSYGKTATFMPKPVVGDNGSGMHVHQSL
                     AKDGKNLFTGNGYAGLSELALYYIGGIIKHARALNAITNPGTNSYKRLVPGFEAPINL
                     AYSSRNRSAAVRVPLVSNPKARRIEVRFPDPITNPYLAFTAMMMAGLDGIQKKIHPGD
                     PVDKNLYDLPPEEAKKITHPCASLDEALECLNK"
     gene            1529..1975
                     /locus_tag="VLL03_00730"
     CDS             1529..1975
                     /locus_tag="VLL03_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSS45930.1"
                     /translation="MKADEILKTAESRAKEMKLPYKGALTPSEAYQLMAEKGAKLVDV
                     RSRAEWDWVGRVPGSVEIELLTYPGMQPNPNFLNQLKQQVGPDSLVLFMCRSGQRSHT
                     AAVAANQAGYSNSYNVLEGFEGDKDGGSHRNSVGGWRAAGLPWVQS"
     gene            2005..2664
                     /locus_tag="VLL03_00735"
     CDS             2005..2664
                     /locus_tag="VLL03_00735"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HSS45931.1"
                     /translation="MSSTSSKPAIACLPNGPYYLLNDLSPAVVPNLQDSKGESLSCVR
                     GVALCRCGGSKNKPFCDGTHGSNGFSDKKLEGGSLNKRVSYQGKRITIHDNRGICSHA
                     GHCTDGLVSVFKMRAEPWIDPDGAAVEKIIETIKRCPSGALSYSISDVEHRDQERPPR
                     VTVTRDGPYAVTGGISLMGQTFGEGASTEHYTLCRCGASKNKPFCDGSHWQVGFKDEH
                     N"
     gene            complement(2724..3863)
                     /locus_tag="VLL03_00740"
     CDS             complement(2724..3863)
                     /locus_tag="VLL03_00740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSS45932.1"
                     /translation="MIGRIPMSITGLAILLFVQSKAGSFALAGVVSALYVLGLAVLAP
                     FLGRLIDRVGPKPVLSVSAVMYPAALTALVLLVTQSAQSFWIAACAAVAGAAMPPITV
                     CMRALYPQLLKEDNLLQTAYSLDSALIEMIFILGPNLVAAFVAIHYTQGAMLFAAASA
                     PLGSLVFLRSPAIKRWKLHAADPQHSLLGPLHDLRLLALFAVTVLYSAAFGLFEVAVT
                     GFATGQGAPAAAGVILGLASVGSALGVLMYGSRDWALPVTKQLLFALAAMAAGILLVV
                     PVTNLYWFTVVAIIGCAPMSVALAATSTLISRLAPRAMLAESFTWMATCLLAGISAGI
                     AAGGILVEHFSPVLVFIAAAAVTALAGIIAWLNLLPIQTQAEEKI"
BASE COUNT          973 a         1178 c         1145 g          746 t
ORIGIN      
        1 tccttgttca gacactccag cgcttcatcc aaagacgcgc agggatgcgt aattttcttc
       61 gcttcttcgg gaggaaggtc atagaggttc ttgtcgaccg ggtcgcccgg gtgaatcttc
      121 ttctggatgc cgtcaagccc cgccatcatc atggcagtaa aggcgagata aggattggtg
      181 atcggatcgg ggaagcgcac ttcgatgcgc cgcgctttcg gattcgacac tagcggcacc
      241 cgcaccgccg cagaacggtt gcgcgatgaa tacgccagat tgatcggcgc ctcgaacccc
      301 ggaaccagcc gcttgtagga attggtgccc gggttggtga tggcgttcaa cgccctggcg
      361 tgcttgatga ttccgccaat gtaataaagt gcgagttccg acagccccgc atagccgttg
      421 ccggtgaaaa gattcttgcc gtccttggcc aacgactggt gcacgtgcat gccggaaccg
      481 ttatcgccga cgacaggctt gggcatgaat gtcgcggtct tgccgtagga gtgcgccaca
      541 ttccagatcg tgtacttgag gatttgcatc cagtcggcgc gcttcaccag gctgttgtac
      601 agggtgccga tttcgttttg ccccgcagcc gccacttcgt gatggtgcac ttcgcatacg
      661 acgccttgct cttccattgc gagcaccatg gcattgcgaa tgtcatgcag actatcggtc
      721 ggcggcaccg gaaagtagcc tcccttcacc ggcgcgcgat gcgccgagtt gccgccttcg
      781 aattcttcgc cggaagacca cggtgcttct tcggatttga ttttgacgaa gcagccggac
      841 atgtccacgt tccaggtcac ggagtcgaaa atgaagaact cgggctccgg accgaaaaac
      901 gcggtgtcgg cgatgcccgt ggatttgaga taagcttcac cgcgcttggc gatcgagcgc
      961 ggatcgcggt catatccctt gccatcggaa ggctcaatca cgtcgcaggt gatattgaga
     1021 accgcctcgt ccgtaaatgg atccatgcgg gcgctgtcag ggtcgggcag caacaccatg
     1081 tcggaggctt caatgctttt ccaaccggca atggatgaac cgtcgaaaaa atggccgtca
     1141 gtaaacttaa gctcgctaaa agccttcacg ggaaccgtga catgctgttc cttgccgcgg
     1201 gtgtcggtaa agcgcaggtc cacaaactta acctcttttt ccttgattat tttgaataca
     1261 tcagcgggcg acgccatttt tctctcctaa gattgaaagt gcggggtatt tttaagattg
     1321 agggaatcgg cagggacttg ctgagcatga aatgtgccac tgccaaaaac gagcaaccta
     1381 ttgtgccaca agtctttagc cgttgaatag cggatttgag cgcactaaag tggcacaaaa
     1441 aaacttcgac cgcaccatta ttgtgcgtaa gtccggccat ggtttttggc tataataggt
     1501 cgtccatcta acacctagcg agcccgccat gaaagccgac gaaatcctga aaaccgccga
     1561 aagccgcgcc aaggaaatga agctgcccta caagggagcg cttaccccca gcgaagccta
     1621 tcagttgatg gccgagaagg gcgcgaaact ggtggatgtg cgctcccgcg ccgagtggga
     1681 ctgggtcggg cgcgttcccg gcagcgtgga aatcgagctc ctcacttatc caggcatgca
     1741 acccaacccc aatttcctaa accagctcaa gcagcaagtg ggcccggatt cgctggtgct
     1801 tttcatgtgc aggagcggcc agcgctctca caccgcggcg gtcgcagcaa accaggctgg
     1861 ctatagcaac agctacaacg tgctcgaagg attcgaagga gacaaggacg gcggcagcca
     1921 ccgcaacagt gtcggcggct ggcgcgcggc gggcttgccg tgggtgcaaa gctaaattaa
     1981 gcggtaagag ggaaaaaagt tgatatgagc agcaccagtt caaaaccggc aatcgcgtgc
     2041 cttcctaacg ggccttacta cctgctcaat gacctctctc ccgctgtagt ccccaatctg
     2101 caggactcca agggcgaaag cctctcttgt gtgcgcggcg tggcgctgtg ccgctgcggc
     2161 ggctccaaaa acaagccttt ctgcgacggt acccacggta gcaacgggtt cagcgacaaa
     2221 aagctcgagg gcggcagctt gaacaaacgc gtgagctacc aaggcaagcg cattaccatc
     2281 catgataatc gcggcatttg ctcccatgcc gggcattgca ctgacgggct tgtctcggtt
     2341 ttcaagatgc gcgcagagcc gtggattgac cccgacggag cggctgttga aaaaatcatc
     2401 gaaactatca aacgctgtcc gtccggcgca ttgagttatt caattagcga tgtagaacac
     2461 cgcgaccagg agcggccgcc ccgagtaacc gtaaccagag acggccccta cgccgtgacc
     2521 ggcggtatct cactgatggg ccaaaccttc ggcgaaggcg cctccaccga gcattacacg
     2581 ctctgccgtt gtggtgcttc caaaaacaag ccgttttgcg acggctccca ctggcaagtc
     2641 ggattcaaag acgagcacaa ctgaaaaact cggaaaagac ggggaaaaat aaatcccctt
     2701 ctgagccgca gtccccgaca aatttaaatc ttttcttcag cttgagtctg tataggcaat
     2761 aaatttagcc aggcaataat ccccgcaagc gcggttactg cagcagcggc aataaacacc
     2821 agcaccggcg aaaaatgctc gaccaggata ccgcctgcgg cgatgccggc gctgatcccg
     2881 gcgagcaggc aggtcgccat ccaggtaaag ctttcagcga gcatcgcgcg cggcgccagc
     2941 cttgaaatca gggtagaggt cgccgccagc gccaccgaca tcggcgcgca gccaataatg
     3001 gcaaccaccg tgaaccaata gagattggta accggcacca ccaataaaat ccccgcagcc
     3061 atggccgcga gcgcaaacag aagctgcttg gtcaccggaa gagcccaatc gcggctgccg
     3121 tacatcaaaa cgccgagcgc gctgcccacg ctggcgagcc ccagtatcac gcccgcggcg
     3181 gcgggcgcgc cttgcccggt cgcgaatccc gtgaccgcaa cttcgaacag gccaaaagcc
     3241 gcggaataaa ggaccgtgac ggcaaaaaga gcgagaagcc gcaagtcatg cagcggcccg
     3301 agcaggctgt gttgcggatc cgccgcatgc agcttccagc gcttgatcgc cggcgagcgc
     3361 aggaagacca aacttcccag cggcgcagag gccgcggcaa acagcatcgc accctgcgtg
     3421 tagtggatgg cgacaaaagc ggcaaccaag ttcgggccca gaatgaaaat catctcgatc
     3481 aacgccgaat cgagcgaata agcggtttgc aacaggttgt cctctttcag caattgcgga
     3541 taaagcgcgc gcatgcaaac cgtgatcggc ggcatggccg cgccggcaac agccgcacac
     3601 gctgcgatcc aaaatgattg agcggattgg gtaacgagca gcacgagcgc ggtaagcgcg
     3661 gccggataca tcaccgcgct cacgcttaaa accggctttg gccccacccg gtcgatcagg
     3721 cggccaagaa acggcgccag caccgccagc cccagaacat aaagcgcgct aaccactccc
     3781 gccaaagcaa aagagcccgc ttttgattgc acgaaaagca gaatcgccag accggtaatg
     3841 ctcatgggaa tgcgcccgat caccgaagcc aggacggcgg gggcgacatg gggtgtgcga
     3901 aaaagtagtt agtagcgttg cagagaaatc atgggactta actgttgcgc aagtccgagt
     3961 ccgggggctg cccccggcaa ttcaagatgc agaccagtta accaagccac cataagaggt
     4021 ggttaacacc actaaaccaa ac
//
LOCUS       DATIOW010000026         3394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_416559, whole genome shotgun sequence.
ACCESSION   DATIOW010000026 DATIOW010000000
VERSION     DATIOW010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416559"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /gene="ssb"
                     /locus_tag="VLL03_00745"
     CDS             complement(<1..266)
                     /gene="ssb"
                     /locus_tag="VLL03_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HSS45933.1"
                     /translation="MASVNKVILIGNLGRDPEVRYNPDGGAMANISIATTDTWKDKQS
                     GEKQERTEWHRIVFFGKLAEIAGEYLKKGSPVYVEGRLQTRKWQ"
     gene            complement(267..1631)
                     /locus_tag="VLL03_00750"
     CDS             complement(267..1631)
                     /locus_tag="VLL03_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSS45934.1"
                     /translation="MTFSDRMSPLELRASMGLASIFGLRMLGMFVILPVFALYAEHLP
                     GGDNRTLVGIALGAYGLTQAILQIPLGWLSDRVGRKAVIYGGLIMFAIGSFVAAAAHD
                     IYLIIFGRVLQGAGAISAAVIAMAADLTREQHRTKAMALIGSTIGLTFALSLVVSPLL
                     NRWIGVPGIFAMTGVLALLAILVILVMPSPQLASSAERDRGKFIAVLRDGQLLRLNYG
                     IFALHAALMALFLIVPFSLRKAGLGVDHHWKIYLPVMLASFVVLVPAIVFAEKKARIK
                     QVFCAAVALMLATQLLLPWLLGSLWEIFVALLAFFAAFNILEAMLPSLISKLAPVGAK
                     GTAIGVYSSVQFFGAFVGAAMGGYLAQHYGNHAVFGFCVLLFAVWFIVAFGMKVPGAV
                     ETKMYPIPAMDTRRASGLSRQLASVPGVKEVLVLAAEGVAYLQVDSGGFDEQNVIRLI
                     AGEI"
     gene            1697..3181
                     /locus_tag="VLL03_00755"
                     /pseudo
     CDS             1697..3181
                     /locus_tag="VLL03_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090680.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
     gene            3114..>3394
                     /locus_tag="VLL03_00760"
     CDS             3114..>3394
                     /locus_tag="VLL03_00760"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 family protein"
                     /protein_id="HSS45935.1"
                     /translation="MIIWLSTVPPTRFPAAKGSASGSARFAALALKCLHQQYPNHISH
                     ALTSDADARPPRSLTPAFYGCYDWHSDVHGHWLLVRLLRLFPDAPFAAQA"
BASE COUNT          744 a         1084 c          935 g          631 t
ORIGIN      
        1 tcttgccatt ttcttgtctg caaccgacct tccacataga ccgggctgcc tttcttgaga
       61 tattcaccgg cgatttcggc gagcttgccg aagaagacta tgcggtgcca ttcagtgcgc
      121 tcctgttttt caccgctctg cttgtccttc caggtatcgg tggtggcgat gctgatgttg
      181 gccattgccc cgccgtcagg gttgtagcgc acctccgggt cgcgcccaag atttccaatc
      241 aggatgactt tgttcactga agccatttaa atctcccccg cgattaatcg gattacattt
      301 tgttcgtcaa aaccgccgct gtccacttgt aaataagcga ccccttccgc ggcaagcacc
      361 agcacttctt ttacccccgg caccgaggcg agctgtcgcg acaagccgct ggcgcggcgg
      421 gtatccatcg ccggtatcgg atacatcttg gtttccaccg cgcccggtac cttcatgccg
      481 aacgccacga tgaaccagac cgcaaacagc agtacgcaaa aaccaaacac agcgtggttc
      541 ccgtaatgct gtgcaagata accgcccatc gccgccccca caaaggcgcc gaagaactgc
      601 acgctgctgt agacgccgat ggccgtgccc ttggctccca ccggcgccag cttggaaatc
      661 agcgaaggca gcatcgcttc gaggatgttg aacgcggcaa aaaacgccaa cagcgccaca
      721 aaaatttccc acaggctgcc cagcagccac ggcagcagaa gctgggttgc cagcatcagt
      781 gccaccgccg cgcaaaacac ctgtttgatc ctggctttct tttccgcaaa cacaatcgcc
      841 ggcaccagca ccacaaaaga agcgagcatc accggcaggt agattttcca atggtggtcc
      901 acacccagtc cggctttccg caacgaaaac ggcacgatga ggaacaaggc catcagcgcc
      961 gcgtgcagcg cgaaaatccc gtaattcagg cgcagcaact gcccatcgcg caacaccgct
     1021 atgaatttgc cgcggtcgcg ctcggcgctc gaggcaagtt gcgggctggg catcaccaaa
     1081 atcacgagga tggcaagcag cgccagcacc ccggtcatcg caaaaatacc cggtacgcct
     1141 atccatcggt tgagcagagg cgaaaccacc agcgacaagg caaaagtgag gccgatggtg
     1201 gagccgatca gcgccatcgc tttggtgcgg tgctgctcgc gcgtcaaatc cgcggccatc
     1261 gcaatcaccg ccgccgaaat cgcgcccgcc ccttgcagca cgcgcccgaa aataatcagg
     1321 taaatgtcgt gggcggcggc cgcgacaaaa ctgccgatgg caaacatgat caatccgccg
     1381 taaatcaccg ctttccgccc cacgcggtcg gaaagccagc cgagcgggat ttgcagaatc
     1441 gcctgcgtca gcccataagc gccgagcgca ataccgacca gggtgcggtt gtcccctccg
     1501 ggaagatgtt cggcgtagag cgcaaacacc ggcagaatca cgaacatacc cagcatgcgc
     1561 aagccaaata tggaggccaa gcccatgctg gcgcgcagct ccagcgggct catcctgtcg
     1621 gagaaagtca tagagaggtt gggaaaaact tgcgaactgc ggtatattaa caggttgacc
     1681 gttgccggaa cagttcatgg acttcatcaa aatccgcggc gcacgcacgc ataatctcaa
     1741 gaacatcaac ctcgatctgc cgcgcaaccg gctcatcgtg attaccggac tgtccggctc
     1801 cggcaaatcc tcgctggcgt tcgacacgct ctacgccgag gggcagcggc gctatgtgga
     1861 atcgctctcg gcttacgcgc gtcaattcct gcagcttatg gaaaaaccgg acgtggattt
     1921 gatcgaggga ctctcgcccg cgatttccat cgagcagaaa gcagcttcgc acaacccgcg
     1981 ctccaccgtc ggcacggtga ctgaaatcca cgattacctg cggcttttgt atgcccgtgt
     2041 gggcgatcct tactgccccg accacaacat cctgctgcag gcgcaaagcg tctcgcaaat
     2101 ggtggatcat gtgctgcaac tgccgctcga caccaggctg atgatacttt cgcccttgat
     2161 cgtcgggcgc aagggcgagc agatggagct tttcgacgag ctgcgcgcac aaggcttcgt
     2221 ccggctcaga atcaacggca aggtgcatga aatcgacgac ctgccgaagc tcaataaaaa
     2281 caaaaagcat acgatcgaag tcgtcgttga ccgggtgaaa gtccgccccg acatcaagca
     2341 gcggctcgcc gaatcgttcg agaccgcgct gcgccatgcc gacgggcgcg cgctggcggt
     2401 ggaaatggaa agcggaaaag agcatttgtt ctcggccaaa ttctcctgcc cggtgtgcag
     2461 ctattccctc cccgagctcg agccgcggct tttctccttc aacaatccca tgggcgcctg
     2521 cccgcgctgc gacgggctgg gacaaatcag ctttttcgat tccaagcgcg tggtcgcctt
     2581 cccgcacttg agtctcgcgt ccggcgccat caagggctgg gatcggcgca accagtttta
     2641 ttttcagatg ctggcgagcc ttgcttcgca ttacaaattc gatctcgacg cgccgtttga
     2701 acagttgccg ccgcccgtcc aggagatggt tctgaacggc agcggccggg aaaaaatccg
     2761 cttcaattat ctcggcgagc gcggcagcca ttatcagcgc gagcacgctt tcgaaggcat
     2821 cattcccaat ttggagcgcc gctaccgcga gaccgattcg ctggtggtgc gcgaggaact
     2881 ggcgaaatat ctcaacagcc agccctgtcc gctgtgccag ggcacgcggc tgcggcgtga
     2941 agcacggcac gtgcgcgtcg ccggcaagac gatttacgaa acgagcgcca tgccgctcaa
     3001 gcaaacggtg cagttcttcg aaaaaataag cctgcacggc gccaagcagg cgattgcgga
     3061 aaaaatcgtc aaggaaatcg tgagccgcct gcaatttctc aacaacgtcg gccttgatta
     3121 tctggctctc gaccgttccg ccgacacgct ttccggcggc gaagggcagc gcatccggct
     3181 ccgcgcgctt tgccgcgctg gcgctgaagt gcctgcacca gcaatacccc aaccacatct
     3241 cgcacgcgct caccagtgac gccgacgcgc ggccgccgcg ttcgctgacg ccggcgttct
     3301 acggctgtta cgactggcac tcggacgtgc acggtcactg gctgctggtg cggctgctgc
     3361 ggctgttccc ggacgcaccg tttgcggctc aggc
//
LOCUS       DATIOW010000027         5048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_925540, whole genome shotgun sequence.
ACCESSION   DATIOW010000027 DATIOW010000000
VERSION     DATIOW010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925540"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VLL03_00765"
     CDS             <1..644
                     /locus_tag="VLL03_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HSS45936.1"
                     /translation="HAAASVQALNENGLHRGLLPMLDLIIEQPLGQRFFAAALKNTDD
                     RVREDKPVSPAFLFAALLWHEVLAAWKNLQSRGEQPMSALHLAMEQVISQQAQRLAIP
                     RRFAATMKEIWTSQPRFLQRAGKRPFRLVEHPRFRAGYDFLLLRCDSGEVDAEIGKWW
                     TAFQNAGQEQRRNMLLIDQTPGKRRRRRRRKGPAQSQDEGVRGPDQPAAPVTE"
     gene            648..1163
                     /gene="folK"
                     /locus_tag="VLL03_00770"
     CDS             648..1163
                     /gene="folK"
                     /locus_tag="VLL03_00770"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HSS45937.1"
                     /translation="MSFNPNQSGVVTAYIALGSNLANPLRQMHDAFEELARLAQSRLA
                     ARSSLYRSAPVSYLNQPDFINAVAGIETALEPHALLTALLEIERRHGRVRDFDNAPRT
                     LDLDLLLYGDLIRHEHGLTLPHPRMHERAFVLQPLLEIAPDCVIPGIGRVADLLVGCA
                     KQKLEKLVEAG"
     gene            1166..1807
                     /locus_tag="VLL03_00775"
     CDS             1166..1807
                     /locus_tag="VLL03_00775"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HSS45938.1"
                     /translation="MLPERYRYIVVEGPIGVGKTSLARLLSQHFSGRALLETPDANPF
                     LPRFYQDMPRYALQTQLFFLFNRVNQLQGLAQMDMFDKLTFADFILEKDPLFARLTLS
                     DDEFRLYHEIYRQVKPQALQPDLVVYLQAPVESLVERVRRRGNAYEQNIPEEYLARIT
                     ESYSRFFHEYNDAPLLIVNSENLNFVDNPADFELLLQRMAGMRGGREFFSLGN"
     gene            1943..2734
                     /gene="panB"
                     /locus_tag="VLL03_00780"
     CDS             1943..2734
                     /gene="panB"
                     /locus_tag="VLL03_00780"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011351027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HSS45939.1"
                     /translation="MRITLNTLQKMRKNGEKIAVLTCYDASFAGLLEGAGVEVLLVGD
                     SLGMVLQGHESTLPVTLNDMAYHTASVARGAKRAFIVTDMPFGTFQLSPQQTFENAVE
                     LMAAGAHMVKLEGGTAMAETVDFLTERGIPVCGHLGLTPQSVHQLGGYQVQGKTDPQA
                     RRLMQEAKMLEQAGAGLLVLEAVPAKLAKEVTRRLSIPTIGIGAGADCSGQVLVLHDM
                     LDVFPGKKPKFVKNFMRGRNSIQAAVETYVREVKAKTFPGPEHSF"
     gene            2827..3666
                     /gene="panC"
                     /locus_tag="VLL03_00785"
     CDS             2827..3666
                     /gene="panC"
                     /locus_tag="VLL03_00785"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HSS45940.1"
                     /translation="MEILSTIESLRARLEREPGVAFVPTMGNLHEGHLALMRQARAHA
                     PCVVASIFVNRLQFGPKEDYDQYPRTLQEDCDKLKACGVDVVFTPDEKELYPSPQQVM
                     VEPPPIADELCGAFRPGHFRGVATVVLKLFNIVQPQVAVLGKKDYQQLFIVRTMVREL
                     NLPIEIVGGETVRAADGLALSSRNGYLSVEERREAVRLHRTLLYIKERVEAGERNFSQ
                     LEQNAKKTLDTHGWRVDYVALRKSSTLEPAKPVDRELVVLGAAWLGGTRLIDSVEFLS
                     GAR"
     gene            3700..4080
                     /gene="panD"
                     /locus_tag="VLL03_00790"
     CDS             3700..4080
                     /gene="panD"
                     /locus_tag="VLL03_00790"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HSS45941.1"
                     /translation="MQRTMLKSKLHRVRVTHSQLHYEGSCVIDQVLMDAADIREYQQI
                     DIYNVSNGERFTTYAIRAERNSGIISVNGAAARKASPGDILIIATYAAYSELELQKYH
                     PELVYVDERNRIIEQRHEIPVQRA"
     gene            complement(4126..4212)
                     /locus_tag="VLL03_00795"
     tRNA            complement(4126..4212)
                     /locus_tag="VLL03_00795"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4176..4178),aa:Leu,seq:caa)
     gene            4265..>5048
                     /gene="queA"
                     /locus_tag="VLL03_00800"
     CDS             4265..>5048
                     /gene="queA"
                     /locus_tag="VLL03_00800"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HSS45942.1"
                     /translation="MQTSDFEFDLPHELIAQFPPDQRSASRMLHLDGKSGELRDAWFR
                     DLPKYVEPGDVMVFNDTRVIKARLFGKKASGGKVQLLVERVLDAHHALAQIYAGHPSR
                     PGTVLNFDRGLQAVVLGREDEFYQVRFESGRTVFELLERHGVTPLPPYIARPAAELDE
                     TRYQTVYAREPGAVAAPTAGLHFDAAMLDNLRERGVKMAYVTMHIGAGTFQPVRTENI
                     AEHKMHGECHSLPVATVKMIEEAKQKDGRVLAVGSTSLRALEA"
BASE COUNT         1069 a         1409 c         1537 g         1033 t
ORIGIN      
        1 gccatgccgc ggccagcgtg caagcgctga acgaaaatgg gctgcaccgg ggtttgctgc
       61 cgatgctgga cttgattatc gagcagccgc tcggacagcg ctttttcgcg gccgcgctca
      121 agaataccga cgaccgcgtc cgcgaggata agcccgtctc ccccgctttt ttgttcgcag
      181 cgttgttgtg gcatgaagtg ctggcggcgt ggaaaaattt acaaagccgg ggcgagcaac
      241 ccatgtctgc gctgcatttg gcgatggagc aggtgataag ccagcaagcg cagcgtctgg
      301 cgatcccgcg gcgctttgcc gcgaccatga aggaaatctg gacgtcgcag ccgcgttttt
      361 tgcagcgcgc cggcaagcgg ccgttccgct tggttgaaca tccccgcttt cgcgccggct
      421 acgattttct gctgctgcgc tgtgacagcg gcgaagtgga tgctgaaatc ggaaaatggt
      481 ggacggcgtt tcaaaacgcc ggccaagaac agcgccgcaa catgctgctg attgaccaaa
      541 cgccagggaa gcgccgtcgt cggcgcagga gaaaaggccc tgcccaaagc caagacgaag
      601 gggtccgcgg ccccgatcaa cccgccgcgc ccgtcacaga ataacccgtg tcgtttaacc
      661 caaaccaatc cggagttgtt accgcctaca ttgcgctggg cagcaatctg gccaatccgc
      721 tcaggcagat gcatgatgcc ttcgaggagc tggcgcggct tgcgcaaagc aggctggcgg
      781 cgcgttcctc gctttaccgc agcgcgccgg tcagttatct caatcagccg gacttcatca
      841 atgccgtggc cgggatcgag acggcgcttg agccgcacgc gctgctcacg gcgcttctgg
      901 aaatcgagcg ccgtcacggc cgggttcgcg atttcgacaa tgccccgcgc acgctggatc
      961 tggatttgct gctgtatggc gatctgatcc gtcatgagca cgggctgact ctcccgcacc
     1021 cgcgcatgca tgagcgggct tttgtcttgc agcccttgct tgaaatcgcg cccgattgcg
     1081 tgatcccggg tatcggccgt gttgccgatc tgctcgtggg ctgcgccaaa caaaaactgg
     1141 aaaaactggt tgaagcgggc tgataatgct gcccgagcgc tatcgctata ttgtggtcga
     1201 agggccgatc ggcgtgggca aaaccagcct ggcgcggctg ctttcgcaac acttcagcgg
     1261 gcgggccttg ctcgaaaccc cggatgccaa cccgtttctg ccgagatttt atcaggacat
     1321 gccgcgctat gcgcttcaga cgcagctatt tttcctgttc aaccgcgtca accaattgca
     1381 gggtttggcg cagatggaca tgttcgacaa gctcaccttc gccgatttca ttctggagaa
     1441 ggacccgctg tttgcgaggc tcactctcag cgacgacgaa ttccgcctgt atcatgaaat
     1501 ctaccggcag gtgaaaccgc aggcgctgca acccgacctg gtggtttacc tgcaggcgcc
     1561 ggtggaaagc ctggtggagc gcgtgcgccg gcggggaaat gcctatgagc aaaacatccc
     1621 cgaggaatat ctggcgcgga ttaccgaaag ttacagccgc tttttccacg agtacaatga
     1681 tgctccgctt ttgattgtca acagcgaaaa cctcaatttt gttgataatc cggcggattt
     1741 tgaattattg ttgcagcgga tggcaggtat gcgcggcggg cgcgaatttt tcagcctggg
     1801 aaattaaatt taccgacggc acggagaaaa acaaatagac aggatttttc gggatttaca
     1861 tgatttatcc ttaaattagt ttctgaaatc acataaatct tgttttagat tttcgtcgcc
     1921 tctatggtaa aaggccttta agatgcgaat caccctcaac accttgcaga aaatgagaaa
     1981 gaacggcgag aaaatcgccg tgcttacctg ctatgacgca agcttcgcgg gcctactcga
     2041 gggcgccgga gtggaggtac tgctggtcgg cgactcgctg ggcatggtgc tgcaaggcca
     2101 tgagtcgacg ctgccggtga cgctgaacga catggcctac cacactgcca gtgtcgcgcg
     2161 cggcgcgaaa cgcgccttca tcgtcaccga catgcctttt ggcacgtttc aactgagtcc
     2221 gcagcagact ttcgaaaatg cggtggagct gatggcggcg ggggcacaca tggtgaagct
     2281 tgaaggcggc acggccatgg cggaaaccgt ggatttcctc accgagcgcg gcattccggt
     2341 gtgcgggcac ctggggctta ctccgcaatc ggtgcaccag ctcggcggct atcaagtaca
     2401 gggcaaaacc gacccgcagg ccaggcggct gatgcaggaa gcgaaaatgc tggagcaggc
     2461 gggcgcgggc ttgctggtgc tggaagcggt gccggcgaaa ctggccaaag aagtcacgcg
     2521 gcggctttcc attcccacca tcgggatcgg tgccggtgcg gactgctcgg gtcaagtgct
     2581 ggtgctgcac gacatgctcg atgtgttccc cgggaagaag cccaagttcg tgaaaaattt
     2641 tatgcgcggc cggaacagca tccaggccgc ggtggaaacc tacgtcaggg aagtaaaagc
     2701 caagaccttt cccggacccg agcactcgtt ttaaccagga taaaggattc gagattgcgg
     2761 atcggtaatc gacaaaccct cacgcttccg cctgctgaat cctgaatcct tgctcttgaa
     2821 tcctccatgg aaattctttc cacaatagaa tccctgcgcg cgaggctcga acgcgagccg
     2881 ggtgtcgctt ttgttcccac catgggcaat ctgcatgaag ggcatctggc gttgatgcgg
     2941 caggcgcgcg cgcatgcgcc ctgcgtggtg gcgagcattt ttgtgaatcg tctgcagttc
     3001 ggcccgaagg aagactacga ccaatacccg cgcaccttgc aggaggactg cgacaaactc
     3061 aaagcttgcg gcgttgacgt ggtgttcacg ccagacgaaa aagaacttta cccctcgccg
     3121 cagcaggtaa tggtggaacc gccgccgatc gccgatgagc tttgcggcgc cttccggccg
     3181 gggcattttc gtggcgtggc gaccgtggtg ttgaagctct tcaacatcgt gcagccccag
     3241 gtcgcggtat tgggcaaaaa agattatcaa caactcttca ttgtccgaac catggtgcgc
     3301 gagttgaacc tgccgattga aatcgtcggc ggcgaaaccg tgcgcgcggc cgacgggctg
     3361 gccttgagtt ccagaaacgg ctatttgagc gttgaagagc ggcgggaagc ggtgcgtttg
     3421 caccgcactc tgctatatat aaaggaacgg gtggaagccg gcgagcgcaa cttttcgcag
     3481 cttgagcaaa acgctaaaaa aacgcttgac acgcatggct ggcgcgtgga ttatgttgcg
     3541 ctgcggaaaa gctctaccct ggagcccgcc aagccggtag acagggagct ggtggtactc
     3601 ggcgccgcat ggctgggcgg cacgcgactt attgatagtg tagagttttt gtctggggca
     3661 aggtagggtc ggacgacctc ttttcgtgcg gagtgactca tgcaaagaac catgctcaaa
     3721 tccaaattgc accgtgtgcg ggtgacgcat tcgcagctcc actatgaagg ctcttgcgtc
     3781 atcgatcagg tgttgatgga tgccgcggat atccgcgagt atcagcagat cgacatttac
     3841 aacgtcagca acggcgagcg tttcaccacc tacgccatcc gcgccgagcg caactcggga
     3901 attatttcgg tgaacggcgc cgccgcgcgc aaagccagcc ccggcgacat tctgattatt
     3961 gcgacttacg ctgcttattc cgaactcgaa ttgcagaagt accatcccga actggtttac
     4021 gtggacgagc gcaaccgcat tatcgagcag cgtcacgaga ttccggtgca gcgggcttga
     4081 ggtttcagtg aagtaaaaag gcggcttgca agccgccttt tttattggtg ccgggggagg
     4141 gactcgaacc ctcatggtgt tgccaccacc ggattttgag tccggcgcgt ctaccgattc
     4201 cgccatcccg gcgagggatt ggggattatc ctctaaaaag cttttttccg gcaagataca
     4261 acctatgcaa accagcgact tcgaatttga tttgccgcat gaactcatcg cgcagtttcc
     4321 gccggatcag cgttcggcga gccgcatgtt acatctggat ggcaaaagcg gtgaattacg
     4381 cgatgcttgg ttccgcgatt tgccgaaata tgtcgagccc ggcgatgtca tggtgttcaa
     4441 cgacacccgc gtcatcaaag cgcggctgtt cgggaaaaaa gccagcggcg gcaaggtgca
     4501 gctgctggtg gagcgggtgc tggacgcgca tcatgcgctg gcgcaaattt acgccggcca
     4561 cccctcgcgc cccggcacgg tcttgaattt tgaccggggc ctgcaagccg tcgtgctggg
     4621 gcgcgaggac gaattttacc aagtgcggtt cgaaagcggg cggacggtat tcgaattgct
     4681 ggagcggcat ggcgtaacac cgctgccgcc ttatattgcg cggcccgcgg ctgagttgga
     4741 tgaaacgcgc taccagacgg tatatgcgcg cgagcccgga gcggtggcgg cgcccaccgc
     4801 cggtttgcat ttcgatgccg ccatgctgga caatctgcgc gagcggggcg tgaagatggc
     4861 ctacgtgacc atgcacatcg gcgccggcac tttccagccg gtgcgtaccg aaaatattgc
     4921 ggaacataaa atgcacggag aatgccatag cctgcccgtt gcgaccgtga agatgatcga
     4981 ggaagcaaag cagaaagacg ggcgcgtgct ggcggtgggc agcaccagcc tgcgcgcgct
     5041 ggaagccg
//
LOCUS       DATIOW010000028         3172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_622115, whole genome shotgun sequence.
ACCESSION   DATIOW010000028 DATIOW010000000
VERSION     DATIOW010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622115"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /gene="rbfA"
                     /locus_tag="VLL03_00805"
     CDS             complement(<1..285)
                     /gene="rbfA"
                     /locus_tag="VLL03_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HSS45943.1"
                     /translation="MHKDYPRSRRVAEQIQRELADIIRLELKDPRVGMITLTGVEVTP
                     DYAHARIFFTQLGESKPETLQGLQRAAGFLRSQLAQRLKMHAVPRLHFVYD"
     gene            complement(362..2872)
                     /gene="infB"
                     /locus_tag="VLL03_00810"
     CDS             complement(362..2872)
                     /gene="infB"
                     /locus_tag="VLL03_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HSS45944.1"
                     /translation="MAQMNVEQFARELKLDPSRLIEQLQAAGVNKQQAEDLLTEQDKS
                     QLLEYLHRVHGAKDQKKKITLTRRQTSEIKKADSTGKARTIQVEVRKKRVFVKRDESA
                     EGEGHDTPPMLDAEQVALREEEARKQAELIARQSEALREKQEKGQKRKQPEVKTEAPE
                     QKQAAPAPGTEGTLHKPAMTPEKAAKADKKTRKQRQVIWQEETARRRTIKTRGDFAGG
                     QGWRERKGPKPRQPQPEGQPLQPHAFSMPTEPVVHEVPVPETITVGVLAQKMSVKAAE
                     VIKVLMNMGTMVTINQVLDQDTAMIVVEEMGHVAKRAKLDDPESFLSAEEQPPQEFPQ
                     QPRAPVVTVMGHVDHGKTSLLDYIRRTRVASGEAGGITQHIGAYHVETPKGTITFLDT
                     PGHEAFTAMRARGAKVTDIVILVVAADDGVMPQTIEAIHHAKAAKVPIVVAINKIDKP
                     EANPDRIKQELLVHEVLPEEYGGEAMFVHVSAKTGQGIDQLLEGVLLQAEVLELKAAK
                     DGPAKGLVIESRLDKGRGPVATILVQSGTLKRGDILLAGAVFGRVRDMFDEAGRTIQE
                     AWPSIPVEIQGLAGVPVAGEPAQVLGDERRAREIALFRQGKFRDVKLARQQTAKLENI
                     FEHMGEGQVKTLSLVIKADVQGSYEALSHALSKLSTGEVKVNIIHSGVGAITESDVNL
                     AFASKAVVIGFNTRADSTARKLIASTGVDVRYYNIIYEAVDEIKAALSGMLAPERKEN
                     VIGLVDIRQVFRISKVGAVAGCYVLEGVVKRNSPVRVLRQNVVIHSGEIESLKRFKDD
                     AREVKAGFECGLSLKGYDDINVGDQLEVYEVVEVARTL"
     gene            complement(2968..3172)
                     /gene="nusA"
                     /locus_tag="VLL03_00815"
                     /pseudo
     CDS             complement(2968..3172)
                     /gene="nusA"
                     /locus_tag="VLL03_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206255.1"
                     /note="modifies transcription through interactions with
                     RNA polymerase affecting elongation, readthrough,
                     termination, and antitermination; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
BASE COUNT          523 a         1011 c          867 g          771 t
ORIGIN      
        1 gtcgtagaca aaatgcagcc gcggcacggc gtgcatttta agccgctgcg ccagttggct
       61 gcgcaaaaac cccgccgcgc gttgcaagcc ttgaagcgtt tccggttttg attcgccaag
      121 ctgggtgaaa aatatcctgg cgtgggcgta atcgggagtg acttcaacac cggtgagcgt
      181 aatcatgccc acgcgcggat ccttcaattc cagccgtatg atatccgcca gttcgcgctg
      241 gatttgctca gcgacacggc ggctgcgggg gtaatccttg tgcatgacaa attctggttg
      301 agagttcaga gttcaaagtt tagagtaaac tgaaaacact taactctcaa ctctcaattc
      361 cctacaacgt tcttgcgact tcgacgacct cgtaaacctc gagctggtcg ccgacattga
      421 tgtcgtcata acccttaagc gacaagccgc actcaaaacc ggctttcact tcacgcgcgt
      481 catccttgaa gcgcttcagt gattcgattt cgccgctgtg gatcaccacg ttttggcgca
      541 gcacccgcac cggcgaattg cgcttgacca ccccttccag cacatagcag ccggcgaccg
      601 cgccgacttt cgaaatgcgg aacacctggc gtatgtccac cagcccgatg acgttttcct
      661 tgcgctcggg ggcgagcatt ccggaaagcg cggcttttat ctcgtccacc gcctcataaa
      721 tgatgttgta gtaacgcaca tccacgccgg tgctggctat cagctttcgg gcagtgctgt
      781 cggcgcgggt attaaagcca atcaccacgg ctttggacgc gaacgccagg ttgacatcgg
      841 attcggtaat cgcgccgaca ccgctgtgga tgatgttgac cttgacctcg ccagtggaaa
      901 gtttcgagag cgcgtgggaa agcgcctcgt aggaaccctg cacgtcggcc ttgatgacga
      961 gcgagagcgt cttcacctgc ccctctccca tatgctcgaa gatgttttcg agcttcgccg
     1021 tctgctgcct agcgagcttc acatcgcgga acttgccttg gcgaaacagt gcgatttcgc
     1081 gcgccctgcg ctcgtccccc agcacctgcg ccggctcgcc cgcgaccggc acgccagcca
     1141 aaccctgaat ttccaccgga atagaaggcc aagcctcctg tatggtgcgc ccggcttcgt
     1201 cgaacatgtc gcgtacgcgg ccaaacaccg cacccgcgag caatatatcg ccgcgtttga
     1261 gtgtgccgga ttgcacgagt atcgtcgcca ccggaccgcg ccctttgtcc aagcgcgact
     1321 cgattaccaa gcccttggcg ggaccgtctt tggccgcctt aagctccagc acctcagctt
     1381 gcagcagcac gccttccaga agctggtcta tcccctgccc ggtcttggca gaaacgtgga
     1441 cgaacatcgc ttcgccgccg tattcctccg gcaacacttc gtgcaccagc agctcttgct
     1501 tgatgcggtc gggattggct tccggtttgt cgattttgtt aatcgccacc acgatcggca
     1561 ctttcgccgc cttggcgtgg tggatggctt caatcgtctg cggcatcaca ccgtcatcgg
     1621 cggccaccac cagaatcacg atgtcggtga ctttcgcgcc gcgcgcgcgc atcgcggtaa
     1681 acgcttcgtg tccaggtgtg tcgaggaagg taatggtgcc tttcggcgtt tcaacgtggt
     1741 acgcgccgat gtgctgggta atgccgccgg cttcgccgct cgccacgcgg gtgcggcgga
     1801 tatagtcgag caaagacgtc ttgccgtggt ccacatgccc catcaccgtc accaccggag
     1861 cacgcggctg ctgagggaat tcttgcggcg gctgctcttc tgcgctcaag aacgattcgg
     1921 gatcgtcgag cttggcgcgc ttggcgacgt ggcccatttc ctcgaccaca atcatcgcgg
     1981 tatcctggtc aagcacctgg ttgatggtga ccatcgtgcc catattcatc agaaccttga
     2041 tgacttccgc cgctttcacc gacattttct gggccagcac gccaacggtg atggtttccg
     2101 gtaccggcac ttcgtgcacc accggttcgg tgggcatgga aaacgcgtgc ggctgcaggg
     2161 gctggccttc cggctgcggc tgcctgggct tgggtccttt gcgctcgcgc cagccctggc
     2221 cgccggcgaa atcgcccctg gtcttgatgg tgcgtctgcg ggcagtctct tcctgccaga
     2281 tgacttgtct ctgcttcctg gttttcttgt ccgccttggc ggctttttcc ggcgtcatcg
     2341 ccggcttgtg cagcgtgccc tcggttccgg gcgcgggagc ggcctgtttc tgctccggcg
     2401 cttcggtttt cacttcaggc tgcttgcgct tttgcccttt ttcctgcttt tcgcgcagcg
     2461 cctcgctttg ccttgcgatc agctcggcct gcttgcgcgc ttcttcctcg cgcagcgcaa
     2521 cttgctcggc atccagcatt ggcggggtgt cgtgtccttc gccttcggca gactcgtcgc
     2581 gcttcacgaa cacgcgtttc ttgcgcactt ccacctgaat cgtgcgcgcc ttgccggtgc
     2641 tgtcggcttt cttgatttcc gaagtctgcc ggcgggtcag cgtgattttc tttttctggt
     2701 ctttcgcgcc gtgcacgcga tgcaagtatt ccagcagctg gctcttgtcc tgctcggtaa
     2761 gcaagtcttc ggcctgctgc ttgttgactc ccgccgcctg caattgctcg atcaagcgtg
     2821 aagggtccag cttcagctct cgcgcaaatt gctctacgtt catctgtgcc atgcgctttc
     2881 ctgcgtttcg agtttacctt ctcatacgaa ccacggcgca cgcgccgtca taatcagctt
     2941 cttcgcgcgt tcggcatcca tgccggtgat ttccagcatt tcagtaagcg gcacataagc
     3001 cacttcctcg agggtgttga aaccttcctg caccaggatt tcggcgactt cctcatccac
     3061 gtcgagccgc tccatgaaca gcttcttgac gccgctcgtt tcttcgttgc tcttcttctg
     3121 cgattcttcg accgtcatga tgttgagctc ccagccggtc aattcggacg cg
//
LOCUS       DATIOW010000029         7424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1030286, whole genome shotgun sequence.
ACCESSION   DATIOW010000029 DATIOW010000000
VERSION     DATIOW010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7424
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030286"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VLL03_00820"
     CDS             complement(<1..874)
                     /locus_tag="VLL03_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HSS45945.1"
                     /translation="MNVNQVALLYLVASVFFIFALKGLSSPVAARRGNLFGIIGMALA
                     VLITLAITRSVSLIVLAIAVGGTVGAVVARRVQMTQMPQLVAAMHSLVGLAAVLIAIA
                     AVNNPAAFNIEVPLPRGNRLELFIGTFVGAITFSGSVIAFGKLAGLGKHFRLFSSAPV
                     VFKGQHWLNLLLAIVMIGFGVVFFMAGEKTWTPFVNMTAIAFVLGVLIIIPIGGADMP
                     VVISMLNSYSGWAAAGIGFSLNNSMLIIAGSLVGSSGAILSYIMCKAMNRSFFSVILG
                     GFGAQEGTAVAGGAQ"
     gene            complement(888..1196)
                     /locus_tag="VLL03_00825"
     CDS             complement(888..1196)
                     /locus_tag="VLL03_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HSS45946.1"
                     /translation="MIDHTIINLTVFVLAVFVGYHVVWNVTPTLHTPLMSVTNAISGV
                     IIVGAILAAGPTEMDFGSIIGFIAVALAAVNVFGGFLVTQRMLEMFKKKMHKAGTKGE
                     "
     gene            complement(1282..2406)
                     /locus_tag="VLL03_00830"
     CDS             complement(1282..2406)
                     /locus_tag="VLL03_00830"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HSS45947.1"
                     /translation="MHIGIPAETLAGEQRVAGTPETVKKMLAGGHHKILVQSGAGGAA
                     SVPDSEYQAAGATIVAGAQELYSLSEIILKVRSPDVDELVQIKKGTILIGLLNPHNKE
                     GIEVLAKQGVTAFAMEALPRITRAQSMDVLSSQANIGGYKAVLVAANHYQKIFPMMMT
                     AAGTVKAARVIVMGVGVAGLQAIATAKRLGAVVEATDVRPETKEQVESLGAKFIEVPL
                     TEEEKELAKGAGGYAREMGGDYKKRQAALTAERIKQADIVITTALIPGRPAPLLVTED
                     MVKTMKPGSVIIDMAAEQGGNCPLTEKDQVVVKHGVTLVGHTNLPARVAADASALYAR
                     NLLNFFDLLVDKKSGNLNLNREDEIIAGTLMCMNGEVVKK"
     gene            2511..4253
                     /gene="mutL"
                     /locus_tag="VLL03_00835"
     CDS             2511..4253
                     /gene="mutL"
                     /locus_tag="VLL03_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HSS45948.1"
                     /translation="MSAIRVLPDLLINQIAAGEVVERPASALKELLENSLDAGAGEIT
                     VQLARGGIKSLKVLDNGSGIVREDLPLALARHATSKITSLDDLERVASLGFRGEALAS
                     IAAVSRLELISRMAKDKHAWKIECAGGIIQKPAPAALSLGTSVEVQDLYFNTPARRKF
                     LKTDATEFAHCEEVFKRIALSRAETAFSLHHNTRAQWHLAAQAAQDRIKALLGENFAD
                     ASVWVDEQSGLLHLWGLAGLPAYSRGTRESQYFYVNRRFVRDKLITHAVREAYQDILH
                     HDRHPAFVLFLEIDPALVDVNVHPTKIEVRFRDSRAVHQFVYHVLNKALSPSVVRHDA
                     LHAAPGNPFTAPTYATQSAMPLGVAQPAVFYQTLFGAQPKTPGGTLGESQQEIPPLGF
                     ALAQLQGIYILAHNDKGLVIVDMHAAHERIMYEKLKSALDNGTTVMQPLLIPVAFNSD
                     KLDVATVEEHQAALRELGFEIAALGPNALAVRAVPTALKDADASELARAVLKDIREYG
                     ASRVLTERRNELLATMACHGAVRANRILSLAEMNALLREMEATERAGQCNHGRPTWYQ
                     FSIADLDKLFMRGQ"
     gene            4381..5292
                     /gene="miaA"
                     /locus_tag="VLL03_00840"
     CDS             4381..5292
                     /gene="miaA"
                     /locus_tag="VLL03_00840"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HSS45949.1"
                     /translation="MGPTASGKTTVALKLVEKFPCEIISVDSAQIYRGMDIGTAKPDA
                     GTLGRVPHHLIDILEPTERYSAAQFRADALKLMREICARGRIPLLVGGTMLYFKALRE
                     GLSELPPADPAMRTVIDAMAKASGWPALHQELSRLDPITAARLEPTDAQRIQRALEIC
                     YITGKPMSQVLGQSEIAALPYRVTAIGFTPGDRAVLHRRIGERFGRMLADGLIGEVES
                     LRSRYDLNRSLPSMRCVGYRQIWQYLDGELDQQQMREKGVAATRQLAKRQLTWLRSMA
                     EVKTFDCLAANLPQKVETYLETILEQK"
     gene            complement(5353..5580)
                     /locus_tag="VLL03_00845"
     CDS             complement(5353..5580)
                     /locus_tag="VLL03_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HSS45950.1"
                     /translation="MVKKSAGDGIYKIVEIVGSSKISWEDAAKRAVQTAARTLHDLRV
                     AEITKLDMKVEDGKVMAYRARVTLSFKYRAD"
     gene            complement(5676..7010)
                     /gene="xseA"
                     /locus_tag="VLL03_00850"
     CDS             complement(5676..7010)
                     /gene="xseA"
                     /locus_tag="VLL03_00850"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HSS45951.1"
                     /translation="MSTQMEIAPRPAVISVAELNRRARDLLEQGFPLLWVAGEISNFT
                     RAVSGHCYFSLKDKEAQVRCVMFKQKTLHLDWKPENGIQVEVRALVTLYEARGEFQLN
                     VETMRRSGLGALYEAFEKLKAKLEAEGLFESSRKKLLPLFPHVVGVITSPQAAALSDV
                     LTTLRRRMPGIPVIIYPAPVQGEGAAQKIAEVLRLAGRRAECDVLIVCRGGGSIEDLW
                     AFNEEIVARAIADCPIPIVSGVGHETDFTIADFVADRRAPTPTAAAELASPNRVELLA
                     ALGLFHKRLQRWMKRLLEARMQQLDYLSRRLQHPAAHLQNQQIHLEQLSLRLKSAWWR
                     QADNRRWRLRELMQKLQVPDTSLFVQKQRDLAQRLKLAGARNLEAALARLQSFKAQLA
                     QLNPQSVLERGYSMVQTVQDEIVRDSAQVRLDDEVKMTFAKGNARARVTGKG"
BASE COUNT         1597 a         2265 c         2057 g         1505 t
ORIGIN      
        1 tctgtgcacc tcccgcgacc gccgtgcctt cctgcgcgcc aaagccgcct agaataacgc
       61 tgaagaagga gcggttcatc gccttgcaca taatatagga cagaatcgcg ccggatgagc
      121 ccaccagcga gccggcgata atcaacatcg agttgttgag cgagaaacca atacccgccg
      181 ccgcccagcc cgaatacgag ttcagcatag agatcaccac cggcatgtcg gcaccgccga
      241 tcgggatgat gatgagcacg ccgagcacga aggcgatcgc ggtcatgttc acgaacggtg
      301 tccaagtttt ctcccccgcc atgaaaaata cgacaccgaa accgatcatg acgattgcaa
      361 gcagcagatt cagccagtgc tggcccttga acaccaccgg tgccgaggaa aagaggcgaa
      421 agtgcttgcc cagacccgcc agcttgccga atgcaatcac cgagccggag aacgtgatcg
      481 caccgacgaa ggtgccgatg aagagctcca gccgattgcc gcgcggcagc ggtacttcga
      541 tgttgaacgc cgccggatta ttgactgcgg caatcgcgat cagcaccgcc gccagaccca
      601 ccagcgagtg catcgcggcg accagttggg gcatctgcgt catttgcact cgccgcgcga
      661 cgacggcacc cactgtgccg cccacggcaa tcgccagcac gatcagcgac acgctcctgg
      721 taatggcaag cgtgatcaag accgccaaag ccatgccgat gatgccgaac aagttgccgc
      781 gccgcgccgc aaccggcgag gacaagcctt tcagcgcgaa tatgaaaaat accgaggcga
      841 cgagatacaa gagagcgact tgattcacgt tcatgacggg ttttccctta ttcgcctttg
      901 gttcccgcct tgtgcatttt ctttttaaac atttccagca tgcgctgcgt caccagaaaa
      961 ccgccgaaga cgttgaccgc cgcgagtgcc accgcaataa aaccgatgat ggaaccgaag
     1021 tccatttcgg tggggccggc ggcgaggatc gcgccaacga taatcacgcc ggaaatcgcg
     1081 ttggtcacgc tcatgagcgg cgtatgcaaa gtgggggtca cgttccacac cacgtgatag
     1141 ccgacgaaca ccgccagcac gaacaccgtg agattaatga tggtgtgatc aatcatattt
     1201 gctccttaac taacaatggg tgaagaagtg ggggaatgaa ttttcacgat acttggccaa
     1261 acacgggctt attcacctca ttcacttttt caccacttcg ccattcatac acatcagcgt
     1321 gccggcgatg atttcatctt cgcggttgag gttgaggttt ccgctcttct tgtccaccag
     1381 caggtcgaaa aagttgagca aattgcgcgc ataaagcgcg ctcgcgtccg ccgccacccg
     1441 cgccggaagg ttagtatggc cgacgagggt gacaccatgc ttgaccacga cctggtcctt
     1501 ttcggtcagc ggacaattgc cgccttgttc cgccgccatg tcgataatca ccgagcccgg
     1561 tttcatcgtc ttgaccatgt cctcggtgac cagcaacggc gccgggcggc cgggaattaa
     1621 cgcggtggta atgacgatgt ccgcctgctt gatgcgctcg gcggtcagag ccgcctggcg
     1681 tttcttgtag tccccgccca tttcgcgcgc gtaaccgccc gcgcccttgg cgagttcttt
     1741 ctcctcttcg gtaagcggca cttcgataaa cttagcgccc agcgactcga cctgctcctt
     1801 ggtttcaggc cgcacgtcgg tggcttcgac caccgcgccc aggcgcttcg ccgtcgcaat
     1861 ggcctgcagt cccgccacgc ccactcccat tacgatcacg cgcgccgctt tgaccgtgcc
     1921 ggcggcggtc atcatcatgg gaaatatctt ctggtaatgg ttcgcagcga ccaaaaccgc
     1981 tttgtaaccg ccgatgttgg cctgcgacga cagcacatcc atgctctgag cgcgggtgat
     2041 gcgcggcagc gcttccatgg cgaacgcggt cacgccctgc ttcgccagca cctcgatgcc
     2101 ttccttgttg tgcggattca aaagcccgat taaaatcgtg ccttttttaa tttgcacaag
     2161 ctcatcgaca tcggggctgc gtaccttgag gatgatttcc gacaggctgt agagttcttg
     2221 cgcgccggca acaatggtcg cgcccgccgc ctggtactct gaatcgggca cgctcgccgc
     2281 gccgccggcg ccggactgca ccagaatttt gtgatgtccc ccggccagca ttttcttgac
     2341 ggtctcaggc gttccggcca cacgctgctc gccggccagc gtctccgcgg gaatgccgat
     2401 atgcattaat caacctcccc aatgttcatt tttcctgttc atttttcgcg aagcgtgaat
     2461 tataagcgaa aaaaggtata ctgcgcttgt ttattcccct tctgcgtttc atgtcggcca
     2521 tccgcgtttt acccgatttg ctgattaacc agatcgccgc cggcgaagtg gtggaacgcc
     2581 ccgcttcggc actcaaagaa ttgctggaaa acagcctcga cgccggcgcc ggcgaaatca
     2641 cggtgcagct tgcccggggc ggaatcaaat cgcttaaagt gctggataac ggcagcggca
     2701 tcgtcaggga agatttgcca ctggctctcg cccgccacgc caccagcaaa atcacctcgc
     2761 tcgatgatct ggagcgcgtg gcaagcctgg gcttccgcgg cgaggcgctt gccagcatcg
     2821 ccgccgtctc aagattggaa ttgatcagcc gcatggcaaa agacaaacat gcctggaaaa
     2881 tcgagtgcgc gggcggcatc atccaaaaac ccgcgcctgc cgcgctgagt ctaggcacta
     2941 gcgtcgaagt ccaggacctt tatttcaata cgccggcgcg gcgcaaattc ctcaaaaccg
     3001 acgccaccga attcgcccat tgcgaagaag tattcaagcg catcgcgcta agtcgcgccg
     3061 aaaccgcttt cagcctgcac cacaataccc gcgcgcaatg gcatctcgcg gcgcaggccg
     3121 cgcaagaccg catcaaggcc ttgctgggcg aaaacttcgc cgacgcctcg gtttgggtgg
     3181 acgagcaatc ggggttattg catctgtggg gattggcggg gcttcccgct tattccaggg
     3241 gcacgcgcga gtcgcaatat ttttatgtca accgccgctt cgtgcgcgac aagctgatca
     3301 cgcacgctgt ccgcgaagcg tatcaggata ttctgcatca cgaccggcat ccggcgttcg
     3361 tgctgttcct ggaaatcgat ccggctctag tcgacgtgaa cgtgcacccg accaagatcg
     3421 aagtgcgttt tcgcgattcg cgcgcggtgc accagtttgt gtatcacgtg ctcaacaagg
     3481 cgctttcgcc ctcggtggtc cggcatgatg ctttgcatgc ggcgcctggc aaccccttca
     3541 ccgcgccaac gtacgccaca caatccgcga tgccgctcgg cgtagcgcag ccggcggtgt
     3601 tttatcaaac cctgttcgga gcgcaaccca aaacgccagg cggcacgctt ggcgaatcgc
     3661 aacaggaaat tccgccgctg ggctttgctt tggcgcaatt gcagggtata tacattcttg
     3721 cgcataacga caagggattg gtgattgtgg acatgcacgc cgcccatgag cgcatcatgt
     3781 acgaaaagct gaaaagcgcg ctggataatg gcacaactgt gatgcagcct ttgctgatac
     3841 cagtcgcatt caactccgac aagctggatg tggctaccgt tgaagaacac caggccgcgt
     3901 tgcgcgagct gggatttgaa atcgcggcgc tgggcccgaa tgccttggcc gtgcgcgccg
     3961 tgcctaccgc cctcaaggac gcggatgcgt cggaactggc gcgggcggtg ctcaaagaca
     4021 tccgcgaata cggcgcaagc cgcgtgctga ctgagcggcg caatgaattg ctggcgacga
     4081 tggcctgcca cggcgcggtg cgcgccaacc gcattttgag cctcgccgaa atgaatgcat
     4141 tgctgcgcga gatggaggcc accgagcgcg ccggccagtg caatcacggc cggccgacat
     4201 ggtatcaatt cagcatcgcg gatctcgaca agctattcat gcgcggccaa taaataatgc
     4261 gtgagaagtg aggagtaagg agtgaggagt aaaaagcaaa aaaacccgga gggtaaggct
     4321 tctacccttc acccctcacc cttcacccct tactcaaaat ggccaccggc cattttgctg
     4381 atgggtccca ccgccagcgg caagaccaca gtggcgctga agctggtgga aaaatttcca
     4441 tgcgaaatca tcagcgtgga ttcggcacaa atttaccgcg gtatggatat cggcacggcg
     4501 aaacccgatg ccggaactct gggacgcgtt ccgcaccatc tcatcgacat tctcgaaccc
     4561 accgagcgct attcggcggc gcaattccgc gcagatgcgc tcaaactgat gcgggaaatc
     4621 tgcgcgcgcg gccgcatacc acttttggtg ggcggcacca tgctctattt caaggcgctg
     4681 cgcgaaggcc tgagcgaact gccgccggct gacccggcaa tgcgcacggt gatcgacgcc
     4741 atggcgaaag ccagcggctg gccggcactg caccaagaac tgtcgcgcct cgatcccata
     4801 accgcggcgc ggctcgaacc cacggatgcg cagcgcatcc agcgcgcgct ggaaatctgc
     4861 tatatcaccg gcaagccgat gtcgcaagtg ctgggacaaa gcgaaatcgc agcactgccc
     4921 tatcgcgtga ctgcaatcgg ctttacaccg ggcgaccgcg cggtcctgca tcggcggatt
     4981 ggcgagcgct tcggacgcat gcttgccgac ggtttaatcg gggaagtgga atcgctgcgc
     5041 agccgctatg acttgaaccg ctcgctgcca tcgatgcgct gtgtcggcta ccgccagata
     5101 tggcaatatc tggatgggga gctcgaccag cagcagatgc gggaaaaagg agtggcggcc
     5161 acccgccagc ttgccaagcg ccagcttacc tggctacgct ctatggcgga agtgaaaacc
     5221 ttcgattgtt tggccgcaaa cctgccgcaa aaggtggaaa cttacctgga aacaatactg
     5281 gagcagaaat agacttcccg aatctggtgt ttcacaaccc tatccgcaac gctaaacgca
     5341 gcacgccgac ctctaatccg cccgatactt gaacgacagc gtcacccgcg cgcggtaggc
     5401 cattactttg ccatcctcga ccttcatgtc gagcttggtg atttcggcca cgcgcaagtc
     5461 gtgcaaggtg cgcgccgcgg tctgcaccgc gcgcttcgcc gcatcttccc aggaaatctt
     5521 gctgctgccg acaatttcaa caatcttgta aatgccgtca cctgcgcttt tcttcaccat
     5581 ggtgtctctc cttcattatt aactagcgcc catttccatc atagaccaca aggaacctct
     5641 gatcacgtgc gacctaatca gaggttccct ggttttcatc ccttgccggt gacgcgcgcg
     5701 cgcgcgttgc ctttggcaaa agtcattttc acttcgtcgt caagccggac ttgcgcgctg
     5761 tcacgcacga tctcgtcttg caccgtctgc accatgctgt agccgcgctc cagcaccgat
     5821 tgcgggttga gttgagccag ctgcgccttg aagctttgca gccgcgcaag tgccgcttcc
     5881 agattgcgcg cgccggcgag cttgaggcgc tgcgccaggt cgcgctgctt ttggacgaat
     5941 agcgaagtat cggggacttg cagcttttgc atgagctcgc gcaggcgcca gcggcggttg
     6001 tccgcctgcc gccaccaggc acttttcaag cgcagcgata attgctccag atggatttgc
     6061 tggttttgca ggtgtgcggc gggatgctgc agccggcgcg aaagataatc gagctgctgc
     6121 atgcgcgctt ccaggaggcg tttcatccag cgctgcaggc gcttgtgaaa cagccccaag
     6181 gccgccaaaa gctcgacacg gttgggactt gccagttccg ccgcggcggt aggcgtgggc
     6241 gcgcgccggt cggcgacaaa atcggcgatg gtaaaatcgg tctcatgccc caccccgctg
     6301 acaataggaa tagggcagtc ggcgatggcg cgcgccacga tttcttcatt aaaggcccac
     6361 aagtcctcga tgctgccccc gccgcggcaa acaatcagca catcgcattc ggcgcgtcgt
     6421 cccgccaaac gcagcacttc cgcgattttt tgcgccgccc cctcgccctg caccggtgca
     6481 ggataaataa ttacgggaat gcccggcatg cgccggcgca gcgtagtcag cacatcggaa
     6541 agcgccgccg cctgcggcga agtaatgacg ccgacgacgt gcgggaacag cggcaaaagt
     6601 tttttgcgcg aggactcaaa cagtccttcc gcttcgagct tcgctttcag cttctcgaac
     6661 gcttcataca gcgcgcccag gcccgagcgg cgcatggttt cgacgttgag ctgaaattcg
     6721 ccgcgcgcct catacagcgt caccagagcc cgcacttcta cctgtatgcc gttttccggc
     6781 ttccagtcga ggtggagggt tttttgcttg aacatcacgc agcgcacctg cgcttccttg
     6841 tctttgagcg agaaataaca atggccggac acggcgcggg tgaagttgga aatttcgccg
     6901 gcaacccaaa gcaatggaaa accctgctcg agcaggtcgc gtgcgcggcg gttaagctcg
     6961 gccaccgaaa tgacggcggg acgcggcgca atttccattt gcgtagacat gcgggaattg
     7021 tactcgatga ctcaaaacaa tttcggatcg cgtggaatcc cgcaccgtta ttagggggtt
     7081 caaaaaacct cccgactggc gcgggattcg aggcgatatc acttgaaatc ccgcgccaga
     7141 caaggttttt cgcgggctga ttcatccaca aacaggaaaa acccgcgatt tttctccggt
     7201 caatgccgtc actccccggc aaagtttata taatatgttg attattataa atatttcatt
     7261 agtataaaat ttaatcggca agcaaaaatc ctttgcacaa aggccattta gcttggcttt
     7321 ttatctgcta tgcacaatgt tatccacaga gaatgtggaa aaataaaaaa tgttttctcg
     7381 cgtcgtcgct ttaattgccg aaagaaagtc cttttggcac gagc
//
LOCUS       DATIOW010000030         4570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1132451, whole genome shotgun sequence.
ACCESSION   DATIOW010000030 DATIOW010000000
VERSION     DATIOW010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132451"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..639
                     /locus_tag="VLL03_00855"
     CDS             31..639
                     /locus_tag="VLL03_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HSS45952.1"
                     /translation="MLHIIDAAGWPIWLIIIASIIALGIIVERAWSLRRDLVAPRVLL
                     LNVINDYQQSGVSEEMLKRLEQGPLLGQIFAAGLKNIRSPREVVKESLDEAGHAAAHE
                     LERFLTSLGTIAAISPLLGLFGTVIGMIEIFGSQTPTGGNPVQLAHGISIALYNTAFG
                     IMVAIPSMIFYRYYRSKAETLMVEMEMQALKLIEIVHGERQE"
     gene            636..1055
                     /locus_tag="VLL03_00860"
     CDS             636..1055
                     /locus_tag="VLL03_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HSS45953.1"
                     /translation="MNFRRGYLKEEPEINLVPLIDVLLVILIFLMVSTTYSKFTELQI
                     NLPQADAEKQQEHPNQINIGVDASGRYIIEKTPLSTTNPEVLSEAMRKAVGNLNDPVI
                     VINADAFATHKAVILVMEAARLAGYTHITFTAERRSN"
     gene            1133..2146
                     /gene="lpxK"
                     /locus_tag="VLL03_00865"
     CDS             1133..2146
                     /gene="lpxK"
                     /locus_tag="VLL03_00865"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HSS45954.1"
                     /translation="MRWMEQYWYRLTFLNLLLLPFALLFAIGVSLRRFLYLIHALPSS
                     RLPAPVVIIGNITAGGTGKTPLVIWLTNFLQEQGWRPGIISRGYGGAAKIPQAVKPES
                     DPVSVGDEPVLLARRCRCPVWIGRNRAAAGKALLTAHPECNVLVSDDGLQHYRLRRDV
                     EIAVVDGERKFGNGLPLPAGPLRELVGRLNSVDAVVVHDGEQKAGAEAIQSSFTMHLA
                     GETFYNLLNPDFKGTAADLRGKKTYAVAGIGNPQRFFRQLQKLGLTVAARPFPDHHLY
                     RPEDLFAPDAEVIIMTEKDAVKCGTFADEKYWVLAVDVQVPRELGLRILKKLRTRHGP
                     QTA"
     gene            2127..2306
                     /locus_tag="VLL03_00870"
     CDS             2127..2306
                     /locus_tag="VLL03_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HSS45955.1"
                     /translation="MDPKLLDILVCPLCKGPLLYKKAEQELICKPDRLAYPIKDDIPV
                     MLEEEARKLRPEEEV"
     gene            2388..3146
                     /gene="kdsB"
                     /locus_tag="VLL03_00875"
     CDS             2388..3146
                     /gene="kdsB"
                     /locus_tag="VLL03_00875"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HSS45956.1"
                     /translation="MIDFIVIIPARLNSIRLPGKPLLDIAGKPMIVHVAEKAAASGAA
                     SVWIATDHQQVAEAVQHRGFNACLTRADHASGTERIAEVARQKAFADDAIIVNVQGDE
                     PLIAPQLIREVARELHDHPEAQVATACTPLHNRGEMFNPNVVKVALDSRGYALYFSRA
                     PIPYARDEFAKNEWPQSLPAYRHVGIYAYRAGFLKTYVGLEPSPLEQFEALEQLRVLW
                     HGYRIVASVTAHATEAGVDTPQDLERVRALLSRK"
     gene            3208..3861
                     /gene="adk"
                     /locus_tag="VLL03_00880"
     CDS             3208..3861
                     /gene="adk"
                     /locus_tag="VLL03_00880"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HSS45957.1"
                     /translation="MRLILLGAPGAGKGTQATYIAQKYGITQISTGDMLRAAVKAGTT
                     IGMEAKKYMDSGELVPDEVIIGLVKERIKQLDCAKGFLFDGFPRTIPQAEALKKAGVR
                     LDYVLEIDVPDEEIVRRLSGRRVHIASGRVYHVLFNPPKVLGKDDVTGEDLVQRDDDR
                     EETVKKRLAVYHSQTKQLIDYFSKWSAQGEKGAPKYVKIPGVGRVEEICDKCFAALK"
     gene            4045..>4570
                     /locus_tag="VLL03_00885"
     CDS             4045..>4570
                     /locus_tag="VLL03_00885"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HSS45958.1"
                     /translation="MKPKNAFYAQSGGVTAVINASACGLIETARKHRTKIGKVYAGRN
                     GIIGALTEDLIDTSKESTAAIRALRHTPSGAFGSCRYKLKGLEQNRVQYERLIEVFKA
                     HNIGYFFYNGGGDSADTCLKVSQLSEQLGYPIVAVHVPKTVDNDLPITDCCPGFGSVA
                     KYVATSIREAGFDVA"
BASE COUNT         1070 a         1323 c         1255 g          922 t
ORIGIN      
        1 acaacctttc ttgaggagga aatttcctaa gtgctccaca ttatcgacgc agccggctgg
       61 ccgatttggc tcatcatcat cgcttccatc atcgcccttg gaatcattgt cgaacgtgcc
      121 tggtcattgc ggcgcgacct cgtcgccccc cgcgttctcc tgctgaatgt cataaacgat
      181 taccagcaaa gcggcgtcag cgaagaaatg ctgaaacgcc tcgaacaggg cccgctccta
      241 ggccagattt tcgccgcagg tttgaaaaac atcagaagcc cgcgcgaggt ggtgaaggaa
      301 tccctcgacg aagccggcca cgccgcggca catgagcttg agcgcttcct caccagcctc
      361 ggcaccatcg ccgccatcag tccgctcctg ggcctgttcg gcacggtgat cggcatgatc
      421 gaaatcttcg gctcacagac gccgaccggt ggcaatccgg tgcaactcgc ccacggcatt
      481 tcgattgccc tctacaacac ggcattcggc attatggtgg cgattcccag catgattttc
      541 taccgctatt accgctccaa ggcggaaacc ctgatggtgg aaatggaaat gcaggccctg
      601 aagctaatcg aaatcgtcca cggcgagcgg caagaatgaa tttccgccgc ggctatctga
      661 aagaggaacc ggaaatcaat ctggtgccgc tgattgacgt gctgctggta attctgattt
      721 tcctgatggt tagcaccacc tattccaagt tcaccgaatt gcaaatcaac ctgccgcagg
      781 cggacgccga aaagcagcaa gagcacccga accaaatcaa catcggggtg gacgccagcg
      841 gccgttacat aattgaaaaa acgccgctca gcaccaccaa tcccgaagta ttgagcgagg
      901 ccatgcgcaa ggctgtgggc aacctcaacg acccggtgat tgtcatcaat gccgacgcct
      961 tcgccactca caaagcggtc atcttggtaa tggaagcggc gcgcctcgcc ggctacaccc
     1021 atatcacatt caccgccgag cgcagatcga attaggaggc tgagggccgg agtctgacaa
     1081 aagcagctct cgaattccca accccgagcc tccaatcccc agtccctcgc atatgcgctg
     1141 gatggaacaa tactggtacc gattgacctt cctgaacttg ctgttgctgc cattcgccct
     1201 gctttttgcg attggcgtaa gcctgcgccg ctttctttac ttgattcacg ccctgcccag
     1261 cagcaggctt ccagcccccg tggtgattat aggcaacatc accgcaggcg gcaccggcaa
     1321 aacccctctg gttatatggc ttacaaattt tctgcaggaa cagggctggc ggccgggaat
     1381 tatcagccgc ggctatggcg gcgcggcgaa aatcccgcag gcggtcaagc ccgaaagcga
     1441 ccctgtttca gttggcgatg agccggtgtt gctcgcgcga cgctgccgct gcccggtgtg
     1501 gataggccgc aaccgcgccg ccgcgggcaa agccttactg acggcgcatc ccgagtgcaa
     1561 tgtgctggtt agcgacgacg gcctgcagca ctaccggctg cgccgcgacg tagaaatcgc
     1621 ggtggtggac ggcgagcgca aattcggtaa tggcttgccg ttgcccgccg ggccgctacg
     1681 cgagctggtc ggcaggctga actccgttga tgcggtggtg gttcatgacg gtgaacaaaa
     1741 agcaggcgcc gaagcaatac aaagctcgtt caccatgcac cttgcggggg aaactttcta
     1801 caatttgctg aaccccgatt tcaaggggac cgctgccgat ttgcgcggga aaaaaaccta
     1861 cgcggtagcg gggatcggca atccgcaacg gtttttccgc caactgcaaa aacttggcct
     1921 gactgtcgcc gcgcgcccat ttcccgacca ccatctttac cgacctgaag atttgtttgc
     1981 ccctgatgca gaagtcatca ttatgaccga gaaagatgcg gtaaaatgtg gcacgttcgc
     2041 ggatgaaaaa tactgggtgc ttgcggtgga tgtgcaagtg cctcgcgagc ttggcctgcg
     2101 gattttgaaa aaactaagga cgcgtcatgg accccaaact gcttgatatc ctggtctgcc
     2161 ccttgtgcaa agggccgctc ctctacaaaa aagccgagca ggagctgatt tgcaagccgg
     2221 atcgactagc ttatcccatt aaggacgata ttccggtgat gctcgaagaa gaagcacgca
     2281 agctccgtcc ggaagaggaa gtgtaaaggc agtgaactgt gaacagtaat cagtaaacgt
     2341 tatctccgag ccactgttca gcgttcaccg tttaccgttc actgaaaatg attgatttca
     2401 tcgttatcat ccccgcgcgc ctgaattcca tccgcctccc cggcaagccg cttctggata
     2461 tcgccggcaa gcccatgatt gtgcatgtcg ctgaaaaagc ggcggccagc ggggctgcca
     2521 gcgtgtggat agccacggac catcaacaag tggcggaagc ggtgcagcat cgtggtttca
     2581 atgcctgcct gacccgcgcc gaccacgctt ccggcaccga gcgcattgcc gaagtcgccc
     2641 gccaaaaagc tttcgccgac gatgcaatca tcgtcaatgt gcagggcgat gagccgctga
     2701 ttgcccccca gctgatacgc gaagtggcgc gcgagttgca cgaccatccc gaagcccagg
     2761 tcgcaaccgc ttgcaccccg cttcacaatc gcggagaaat gttcaatccc aacgtggtca
     2821 aggtcgcatt ggacagccgg ggctatgcgc tttatttctc gcgcgcgccc attccttatg
     2881 cacgcgacga attcgccaaa aacgaatggc cgcaaagttt gccggcatac cgtcatgtcg
     2941 gcatttatgc gtaccgcgcc ggctttctga aaacctacgt cggactcgag ccttcgccgc
     3001 tggagcaatt cgaggcactg gagcaattgc gcgtgctgtg gcatggctac cgcatcgtgg
     3061 caagcgtcac cgcgcacgcc acggaggccg gcgtggacac gccccaagac ctggagcggg
     3121 tgcgcgcgct gctgtcgcgc aaatgacgcc cctgttcgcg ccgattccag taaaatcgga
     3181 aaaaatcaac caaggggcct gaaaatcatg agactcattt tgttgggtgc gcccggcgcg
     3241 ggcaaaggca cccaggctac ctatatcgcg cagaaatacg gcataaccca gatttccact
     3301 ggcgacatgt tgcgcgccgc ggtcaaggcg gggacaacaa tcggcatgga agcgaaaaaa
     3361 tacatggatt caggcgagct ggtgccggat gaggtcatca tcggcctggt caaggagcgc
     3421 atcaagcagc tcgattgcgc caaaggtttt ctgtttgatg gttttccccg caccatcccg
     3481 caggcggagg cattgaaaaa agcgggagtg agactggatt acgtattgga aatcgacgtg
     3541 ccggatgagg aaatcgttcg ccgtttgagc gggcgccgcg tgcacattgc ttcgggacgc
     3601 gtttatcatg tcttatttaa tccgcccaaa gtcctgggca aagacgacgt caccggcgaa
     3661 gacctggtgc agcgcgatga cgacagggaa gaaaccgtga aaaagcgcct tgccgtttat
     3721 cactcccaaa ccaaacagct tattgattac ttctccaaat ggtcggcgca aggcgagaaa
     3781 ggcgcaccca aatacgtgaa aattcccggt gtcggcaggg ttgaagagat ttgcgacaag
     3841 tgttttgcag cattgaaata agataattga accgcagaga cgctgaggcg cagagaaaaa
     3901 accaaacttg agtttttcaa gatgaaattt accagtgctt atcatggggt atcaataaaa
     3961 cgatatttgt acacttgaaa taataagaaa tagtttttga atttctccgt gtctctgcgc
     4021 ctctgcggtt gggagatttg aatcatgaaa ccgaaaaacg ctttttacgc ccaatccggc
     4081 ggggtcaccg ccgtcatcaa tgcttccgcc tgcggcttga ttgaaactgc gcgcaaacac
     4141 cgcaccaaaa tcggcaaggt ctacgccggc aggaacggta tcatcggcgc gctcaccgaa
     4201 gacctgatcg acaccagcaa ggagtccacc gcggctatcc gcgcattgcg gcacacgcct
     4261 tcaggcgcat tcggctcctg ccgttacaag ctcaagggtc tggagcagaa ccgcgtgcaa
     4321 tacgagcgct tgatagaggt gttcaaggcg cacaacatcg gttatttctt ctacaacggc
     4381 ggcggcgatt cggcggacac ctgcctcaaa gtttcgcagc tttcggaaca actgggctac
     4441 cccattgtcg cggtgcacgt gccgaaaacc gtggacaacg atttgccgat taccgactgc
     4501 tgcccgggat tcgggtcagt cgcgaaatac gtggccacca gcatccgcga agcgggcttt
     4561 gacgtcgcct
//
LOCUS       DATIOW010000031         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_726362, whole genome shotgun sequence.
ACCESSION   DATIOW010000031 DATIOW010000000
VERSION     DATIOW010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726362"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VLL03_00890"
     CDS             <1..736
                     /locus_tag="VLL03_00890"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HSS45959.1"
                     /translation="PGRGMEREVIGTRRVSAKTRLGIYSSAYRLRLLEALDTDYPALR
                     VLAGDDEFEKMGYAYIENHPSPYYNMRWYGGELAEFLKTTPPYNEHPVLSEMAAWEWA
                     MALAFDAPNEPVVTVEDMGKVPADAWPNLVFTPHASLQRLNLEWNVPAFWKAVDKKQQ
                     PEPPLKNEFPIGWVLWRKELQSYYRSLSVDEAWALDAMREGQNFAEICSGLCEWIDEL
                     QVAQHAAGLLKGWIAEGMISKISFSR"
     gene            complement(763..1212)
                     /gene="ssb"
                     /locus_tag="VLL03_00895"
     CDS             complement(763..1212)
                     /gene="ssb"
                     /locus_tag="VLL03_00895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HSS45960.1"
                     /translation="MASVNKVMIIGNLGRDPEVRYMPDGGAIANISVATTDTWKDKSG
                     EKQERTEWHRVAFFGKLAEIAGEYLKKGSQVYVEGSLRTRKWQDKEGKERYTTEIVAD
                     RMQMLGSRAGGSESMAREEAPPKESAGAAKSGGKGGGIEDLEDDIPF"
     gene            complement(1213..>2169)
                     /locus_tag="VLL03_00900"
     CDS             complement(1213..>2169)
                     /locus_tag="VLL03_00900"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSS45961.1"
                     /translation="ALIGATIGLTFALSLVAAPLLNQIIGVPGIFVMTGVLALLAIAV
                     VYAVIPNPQHSYSSASDAQRARLLAVLRDGQLLRLNYGIFALHAVLMALFMVVPFALR
                     KAGLGVDRHWLMYLPVMLGSFVLLLPAILFAEKKARLKQVFCASVVLMLATQLLLPWL
                     LGSLWEIFVALLAFFTAFNILEASLPSLISKIAPLGAKGTAIGVYSSVQFFGAFVGAA
                     VSGYLSQHYGTKAVFAFGAVLLAIWLIPALTMKVPVAVETKIYPIPAMDNRRASGLSR
                     QLVSVPGVQEALVLAAEGVAYLKVDSNGFDEQNVIRLIAGEV"
BASE COUNT          495 a          663 c          602 g          409 t
ORIGIN      
        1 gcccggccgg gggatggaac gcgaagtgat tggcacccgg cgcgtcagcg ccaaaacacg
       61 gcttgggatt tattccagcg cttaccgcct gcggctcttg gaagcgctgg acaccgatta
      121 ccctgccctg cgcgtgctgg caggtgacga tgagttcgag aagatgggat atgcctatat
      181 agaaaaccac ccttctccct attacaacat gcgctggtac ggcggcgaac tcgcggaatt
      241 tctgaagacc acgccgccct acaacgagca tccggtgtta agcgaaatgg cagcctggga
      301 atgggcaatg gcgctcgcct tcgatgcacc gaacgagccg gtggtcacgg tggaagacat
      361 gggcaaagtg ccggcggacg cctggccaaa tctggtgttc acgccccacg cttcgctgca
      421 gcgcttgaac ctggaatgga acgtgccggc gttctggaaa gcagtggata aaaaacaaca
      481 gcctgaaccc ccgctgaaaa acgagtttcc tataggctgg gtgctgtggc gcaaggaact
      541 gcaaagttat taccgctccc ttagcgtcga tgaggcctgg gcgctggacg cgatgcggga
      601 aggacagaac ttcgctgaaa tttgcagcgg gctttgcgaa tggatagatg aacttcaggt
      661 cgcgcagcac gccgcgggtc tgctcaaagg ctggatagcc gaagggatga tcagcaagat
      721 ttcgttttcc cgctgaattc cgtactacgt ccggcacagc gatcagaacg gaatatcgtc
      781 ctctaaatct tcaattccgc cgcccttgcc ccccgacttg gctgcaccgg cggactcttt
      841 tggcggcgct tcttcgcgtg ccatgctctc agaaccgcca gcacggctgc ccagcatctg
      901 catgcggtcc gcgacaattt cagtggtgta gcgttctttt ccctccttgt cctgccactt
      961 gcgggtgcgc agcgagcctt cgacatacac ctggctgcct tttttcagat actctccggc
     1021 gatttcggcg agcttgccga agaacgccac gcggtgccat tcagtgcgct cctgtttctc
     1081 tccgctcttg tccttccagg tatcggtagt agcgacgctg atgttggcga tagccccgcc
     1141 gtcgggcatg tagcgcacct ccgggtcgcg cccgaggttg ccgatgatca taaccttgtt
     1201 cactgaagcc atttaaacct cccccgcaat taaccgaata acattctgtt cgtcaaaacc
     1261 gttgctgtcc actttgagat acgccacgcc ttcggcggcg agcaccagcg cttcttgcac
     1321 tcccggaacc gagacgagct gccgcgacaa tccgctggcg cggcggttgt ccatcgccgg
     1381 aatcggatag atcttggttt ccacggcaac cggcactttc atggtgagcg ccggtatcag
     1441 ccaaatggcc agcagcacag cgccgaaagc gaacaccgcc ttcgtgccgt aatgctgcga
     1501 cagatacccg ctcaccgcgg cgccgacaaa agcaccgaaa aactgcacgc tgctgtacac
     1561 gcctatcgca gtgcccttgg cgccgagcgg cgcgattttt gaaatgagcg aaggcagcga
     1621 cgcttcgagt atattgaaag cggtaaagaa agcgagcaac gcaacaaaga tttcccacag
     1681 actgccgagc agccacggca gcaataactg cgtggcgagc atcagcacca ccgacgcgca
     1741 aaacacctgc ttgagcctgg ctttcttttc cgcaaacaaa atagccggca gcagcagcac
     1801 aaaagaaccc agcatcaccg gcagatacat gagccagtgg cggtcaacgc caagccctgc
     1861 cttgcgcaaa gcaaacggca ccaccataaa caaggccatc agcaccgcgt gcagcgcgaa
     1921 gatgccgtaa ttcaggcgca gcaactggcc atcgcgcaat accgcgagca gcctggctct
     1981 ctgggcatcg ctcgctgaag aataactgtg ttgcggattg ggaatcaccg cgtagaccac
     2041 ggcaatcgcc aacaacgcca gcacaccggt catgacaaaa atacccggca cgccgatgat
     2101 ctgattaagc aacggcgcag cgaccaatga aagcgcgaag gtgagtccga tggtcgcgcc
     2161 gatcagcgc
//
LOCUS       DATIOW010000032         2888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_217956, whole genome shotgun sequence.
ACCESSION   DATIOW010000032 DATIOW010000000
VERSION     DATIOW010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217956"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2303)
                     /locus_tag="VLL03_00905"
     CDS             complement(<1..2303)
                     /locus_tag="VLL03_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HSS45962.1"
                     /translation="MSYPPVKQGLYDPAREHDACGVGFIAHTKGAKSHAIIEKGLQIL
                     INLTHRGATGWDPLLGDGAGILIQLPDAFLRAECGKLKRALPKSGGYGVGMVFLPRTT
                     DSRAACEKLMEQIVAEEGQEVLGWRDVPTSNQGISEDAKRQEPLACQIFVARGSSCPD
                     QDSFERKLFVIRKRIEHAVRSLNLADGKMFYISSLSSRTLVYKGMLLANQVGTYYKDL
                     QDERMVSALALVHQRFSTNTFPTWELAHPFRMIAHNGEINTLRGNINWMAARRHAISS
                     PLLGKDLEKLWPLIAEGQSDTACFDNALELLVAGGYSLAHAMMLLIPEAWAGNPLMDE
                     SRRAFYEYHAALMEPWDGPAAVAFTDGRQIGATLDRNGLRPARYLITDDDMVVMASEM
                     GVLDIPQEKIIKKWRLQPGKMFLIDLEAGRIIDDAELKARLSSAKPYRAWIDQSKIAL
                     EELPGQEYQIEAGATLLDRQQAFGYSQEDLKFILAPMAAVGEEAVGSMGNDSPLAVLS
                     DRPKPLYNYFKQMFAQVTNPPIDSIREELVMSLVSFIGPRPNLLGIDVTTPNQRLEVK
                     QPVLTSEDMGKILRIAEHTKGAFRSCVLSICYPAAQGAAGVEKALDALCQKAEQAVRR
                     DCNIIVLTDKEVGAENVAVPALLATAAVHHHLVRNGLRTSTGLVIETGSVREVHHYAL
                     LAGYGAEAIHPYLAFETLAHLNDFAPRDLNYKEAVKRYIKAVNKGILKVMSKMGISTY
                     QSYCGAQIFEAVGLNSTFVQKYFTGTA"
     gene            complement(2506..>2888)
                     /locus_tag="VLL03_00910"
     CDS             complement(2506..>2888)
                     /locus_tag="VLL03_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203815.1"
                     /note="dGTPase family type 2 subfamily; presumably
                     hydrolyzes dGTP to deoxyguanosine and triphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HSS45963.1"
                     /translation="LVSDLTTQSLKNIRHEAPVNLDEVRKTGSLIAFSLEIREEQQQL
                     KQFLRRHLYQHYRVARMSGKARRIIADLFRAFMEDARLLPPQFQDKAGGDKPRAIADY
                     IAGMTDRYAIREHRRLFSVEELWS"
BASE COUNT          557 a          833 c          870 g          628 t
ORIGIN      
        1 ctggcggtgc cggtgaaata tttctgcaca aaggtggagt tcagccccac cgcctcgaaa
       61 atctgcgcac cgcagtagga ctggtaagtg gaaatgccca tcttggacat cactttgaga
      121 atgcctttgt tgacggcctt gatgtagcgc ttgaccgctt ccttgtagtt caagtccctg
      181 ggcgcgaaat cgttcagatg cgccagcgtc tcaaatgcca gataaggatg aatcgcttcg
      241 gcgccgtagc cggcgagcaa ggcgtagtgg tgcacttcgc gcaccgaacc ggtttcaatc
      301 accagaccgg tgctggtacg taggccattg cgcaccagat gatgatgcac agccgcggtg
      361 gcaagcagcg ccggaaccgc cacgttttcc gcaccaacct ctttatcggt aagcacgata
      421 atattgcagt ctcgacgcac ggcttgttcc gctttttggc acagggcatc gagcgctttt
      481 tccactcccg ccgcgccttg cgccgcggga tagcaaatgc tcaaaacgca ggagcggaac
      541 gcacccttgg tgtgttcggc gatgcgcagg atcttgccca tgtcttcgct cgtcagcacc
      601 ggttgcttga cttccagccg ctggtttgga gtggtgacgt caatgcccag tagattcgga
      661 cgcgggccga taaaggaaac cagcgacatc accaattcct cccggatgga atcgatcggc
      721 ggattggtca cctgcgcaaa catctgcttg aaatagttgt aaagcggttt cggccggtcg
      781 gagagtacgg ccagtggcga atcgttgccc atcgagccca cggcttcttc acccaccgcc
      841 gccattggcg cgaggatgaa tttcaggtct tcctggctgt agccgaaagc ctgctggcgg
      901 tcgagcagtg tagccccggc ttcaatctgg tattcctgcc caggcaattc ttccagcgcg
      961 attttggatt ggtcgatcca ggcgcgataa ggcttggccg aagaaagccg cgctttcaat
     1021 tcggcatcat cgatgatgcg gccggcttcg aggtcgatga ggaacatctt gcccggctgc
     1081 agccgccatt tcttgatgat tttttcctgc gggatgtcga gcacacccat ttccgaggcc
     1141 atcactacca tgtcatcgtc ggtgatgaga tagcgcgccg gcctgaggcc gttgcggtcg
     1201 agcgtcgcgc cgatttgccg cccgtcggtg aacgccaccg cggcaggacc gtcccatggc
     1261 tccatcaacg ccgcatgata ttcgtagaac gcacgccggc tttcatccat cagtggattg
     1321 ccggcccagg cttcgggaat gagcagcatc atggcatgcg ccagcgagta gccgcccgcg
     1381 accagcaatt ccagcgcatt gtcgaaacac gcggtatccg actggccctc ggcaattaaa
     1441 ggccacaact tctcgaggtc tttgcctaaa agcggcgagg aaatggcgtg gcggcgcgcc
     1501 gccatccagt tgatgttccc gcgcagcgtg ttgatttcac cgttgtgagc gatcatgcga
     1561 aacggatgcg cgagctccca agtcgggaaa gtgttggtgg agaaacgctg gtgcacgaga
     1621 gccaaggcgg aaaccatgcg ctcgtcctgc aagtctttat agtaggtgcc cacctgattc
     1681 gcgagcagca tgcctttgta caccagggtg cggctcgaca gcgaggagat gtaaaacatt
     1741 ttgccgtcgg caagattgag cgagcgcacc gcgtgttcga ttctcttgcg gatgacgaag
     1801 agttttctct cgaagctgtc ctggtccggg cagctgctgc ctctggcgac gaaaatctgg
     1861 cacgccagcg gttcctgccg cttggcgtct tcactgatcc cctggttgct ggtgggtaca
     1921 tcgcgccagc cgagcacttc ctggccttct tcggcgacga tttgctccat gagtttttcg
     1981 caggcggcgc ggctgtctgt cgtgcgcggc agaaacacca tgcccacgcc ataaccgccg
     2041 ctcttcggca gcgcgcgctt cagttttccg cattcggcgc gcaggaaagc gtcgggcagc
     2101 tgaatcagga tgcctgcgcc gtcgcccaag agcgggtccc agccggtcgc gccccggtgg
     2161 gtgaggttga tcagaatttg caaacccttc tcgataatgg cgtggctttt tgctcccttg
     2221 gtatgggcaa taaagccgac cccgcaagca tcatgctcgc gggcgggatc gtacaaaccc
     2281 tgttttacgg ggggatagct caccttttac ctcgtgaatc tgcggtcagg acgacaaagc
     2341 cggactttaa atagcgggca tctcgggatt tcgctaggcc cggaatttaa agcggaagca
     2401 tttaagttta catcttcccc tgggctgctt caacttggac gctgcagggt tgtggccgcg
     2461 tgacagggag ggccgccggt ccggaaaggc ctgatgggaa atggcttatg accacagctc
     2521 ttccacggag aacaggcgcc ggtgctcgcg gatggcgtag cggtcggtca tgccggcaat
     2581 gtagtcggca atcgctcttg gcttatcgcc gcccgccttg tcctggaatt gcggcggcag
     2641 caagcgagcg tcctccatga aagcgcgaaa caggtcggcg atgatgcgcc gggctttacc
     2701 gctcatgcgc gcaacgcggt aatgctggta gaggtggcgg cgcagaaact gcttcaattg
     2761 ctgctgctcc tcgcggattt cgaggctgaa ggctatcagc gacccggttt tccgcacctc
     2821 atccagattg acgggagctt cgtggcggat gtttttcaag ctctgggtgg tgaggtcgga
     2881 caccaggg
//
LOCUS       DATIOW010000033         8613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_319588, whole genome shotgun sequence.
ACCESSION   DATIOW010000033 DATIOW010000000
VERSION     DATIOW010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8613
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319588"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VLL03_00915"
     CDS             complement(<1..200)
                     /locus_tag="VLL03_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003270147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HSS45964.1"
                     /translation="MQPAAQIRMWTEGVPVEAEALRQLENMAKLPVLAGHIAVMPDVH
                     LGKGATVGSVIPTKGAIIPAAVG"
     gene            complement(208..783)
                     /locus_tag="VLL03_00920"
     CDS             complement(208..783)
                     /locus_tag="VLL03_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HSS45965.1"
                     /translation="MSTPLVLTLDVAGLPRRWIDTQEAVLYYARELVTWSIGDPVCVY
                     HGGISRLTGKRSEIAPRPIIAVEGKVHTGHFECEPWLNRETLFRRDRHMCAYCGGLYH
                     AHELTKDHVTPTSRGGVEHWTNVVTACRMCNEVKSNRTPEEAGMKLLYVPYAPNRYEG
                     MILANRHILADQMDYLLAHVPKNSRLWVGRA"
     gene            complement(1244..1537)
                     /gene="ftsB"
                     /locus_tag="VLL03_00925"
     CDS             complement(1244..1537)
                     /gene="ftsB"
                     /locus_tag="VLL03_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HSS45966.1"
                     /translation="MKFFTLILIALIAAIQYPLWLGKGSWLRVWEIDRQLKEQRATNR
                     GLQARNGALDAEVRDLKQGFEAIEERARSELGMIKQNEIFFQILDEPQQKSRE"
     gene            complement(1534..2820)
                     /gene="eno"
                     /locus_tag="VLL03_00930"
     CDS             complement(1534..2820)
                     /gene="eno"
                     /locus_tag="VLL03_00930"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HSS45967.1"
                     /translation="MSAIVDVVAREILDSRGNPTVEADVLLESGVLGRAAVPSGASTG
                     TREAMELRDGDKKRYFGKGVLKAVEHINTEICEAIIGLDATEQSFIDRTLIDLDGTEN
                     KSRLGANAILAVSVAVAKAAAEEAGLPLYRYFGGSGQMQMPVPLMNVINGGAHANNNI
                     DLQEFMLIPLGAKSFREALRYGAEVFHTLKKLIDEKGMPTTVGDEGGFAPSLPNNEAA
                     LKLIVEAIEKAGYQPGADVAIGLDCASSEFFNEGKYRLESEKLNLNSKQFSDYLAAWT
                     DKYPIISIEDGMAETDWDGWRTLTRRLGTSVQLVGDDVFVTNTKILAEGIKQEIANSV
                     LIKINQIGTLTETFAAIELAKHSGYTVVISHRSGETEDTTIADVAVATNAMQIKTGSL
                     SRSDRLAKYNQLLRIEEDLGDSAVYAGRGAFYQLRR"
     gene            complement(2820..3692)
                     /gene="kdsA"
                     /locus_tag="VLL03_00935"
     CDS             complement(2820..3692)
                     /gene="kdsA"
                     /locus_tag="VLL03_00935"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HSS45968.1"
                     /translation="MKLCGFDAGLDKPLFLIAGPCVVESRQLALDTAGALKEICREIG
                     VPFIFKSSYDKANRSSGKSFRGIGIDNGLKILDDVKNQIKVPVLTDVHEAADVAQVAA
                     VVDVLQTPAFLCRQTDFIHAVAATGKPVNIKKGQFLSPGEMKNVVDKAREASGKDNIM
                     VCERGVSFGYNNLVSDMRSLMIMRETGCPVVFDATHSVQLPGGQGNKSGGQREFVPVL
                     ARAAVASGIAGIFMETHPDPDQALSDGPNAWPLKYMRELLQTLQALDELVKRRGFAEQ
                     ALTEKTIKPTRPSK"
     gene            complement(3689..5356)
                     /locus_tag="VLL03_00940"
     CDS             complement(3689..5356)
                     /locus_tag="VLL03_00940"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HSS45969.1"
                     /translation="MTKYIFVTGGVVSSLGKGIAAASLAAILESRGIKVTLLKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFVNAKMSKRNNFTAGQIYESVIKKE
                     RRGEYLGRTVQVIPHITDEIKSFIAKGAQGAEVAIVEVGGTVGDIESLPFLEAIRQLG
                     LELGRDNTCYVHLTLVPFIKSAGEMKTKPTQHSVKELREIGIQADALLCRSDRPIPED
                     ERKKIALFTNLAPNAVISVWDVDSIYKIPYMLHQQILDEIVCHKLQILAKPADLKIWK
                     RLIDALEHPQHEVSIAMVGKYVDLTDSYKSLNEALVHAGIHTRSKVKIQYVDSEAIEK
                     DGIDCLKKVDAVLVPGGFGKRGVEGKIKAIRYARENKIPYLGICLGMQLAVIEYARHK
                     AGLTQANSTEFEPDTAQPVIALVTEWQNRDGRAERRDAHSDLGGTMRLGAQTADIISD
                     TLVQRIYNSNIVSERHRHRYEVNNFYLPKLEAAGLKIAARTKAEQLCEMIELPDHPWF
                     VACQFHPEFTSTPRAGHPLFTAFIEAALRHSGAFTESEGAKQLSVAR"
     gene            5493..6515
                     /locus_tag="VLL03_00945"
     CDS             5493..6515
                     /locus_tag="VLL03_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HSS45970.1"
                     /translation="MHAIWETHLRNTGANLTDSIVRDFGDLRAELRAARDGTVLCDLS
                     QYGLLLFAGEEAQAFLHAQFSSDVKKIHLAAAQYSSYCTAKGRMLANFLLWQSNEGFF
                     AQLHAGLLESIQNRLTKFVLRTKVKISNVSDSHVRLGLAGIQAEALVKKHLGSLPPAR
                     LGVAHEKAVTLIRLSPNRFEIIVPHQEAPKLWDALRTDAKPAGSACWDWLNVRDGVPV
                     ITAATQEQFVPQMANLELLGAVSFNKGCYPGQEIVARTQHRGLLKRRMYLAHVDIEAS
                     PQPGDELFSQDIESQASGIIVNAAAAPEGGYDVLAVVQISSAQSDEVRWKSMGGAALK
                     LLPLPY"
     gene            6545..6826
                     /locus_tag="VLL03_00950"
     CDS             6545..6826
                     /locus_tag="VLL03_00950"
                     /inference="COORDINATES: protein motif:HMM:NF027614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4936 family protein"
                     /protein_id="HSS45971.1"
                     /translation="MPLNFYIYYRVEQAHAASLEPLIHAMQAVLWKQTGVAGRLLKKR
                     DEPLLWMEIYENVAEAGPFEDALMHAVEKYQVKQFVEAGSQRHTECFVI"
     gene            6843..7610
                     /locus_tag="VLL03_00955"
     CDS             6843..7610
                     /locus_tag="VLL03_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HSS45972.1"
                     /translation="MLAYKLSPDWPLLLAANRDEFYERSTAAAAFWEDHPEVLGGRDL
                     QKHGTWLGVTRSGRFAAVTNFRQGFAQKKNAPSRGALVSNYLLGKEKPGDYLQRLQQH
                     AAEYDGFNLIVGDIKEVYYCSNRGGKMQKLAPGLYGLSNHLLDTPWPKVERGKKQLIR
                     LFSAPQPPSVEALFAILADRFVPEDKELPNTGIGPEWERRLSSAFTATAEYGTRSSTL
                     LFIHSDNRVELIERSFKRAGAEGGTVEHEFLIDSTPL"
     gene            complement(7605..8030)
                     /locus_tag="VLL03_00960"
     CDS             complement(7605..8030)
                     /locus_tag="VLL03_00960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSS45973.1"
                     /translation="MQDYRIREVDWQEAQHELKRVREAVFIVEQNVPVELEWDGLDPN
                     ARHVLAQSRGGVAMGTARLLPDGQIGRMAVLKEWRGLGVGSALLEALLQIARSRGFSQ
                     VFLNSQTRAVAFYSRRGFKVASGEFMDAGIPHVKMVLHL"
     gene            8090..>8613
                     /locus_tag="VLL03_00965"
     CDS             8090..>8613
                     /locus_tag="VLL03_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HSS45974.1"
                     /translation="MKSLWNNKEAAQHKGDLGLRVYTSRLLGRDKSLVLHGGGNTSVK
                     IKERNILGAEEQILYVKGSGWDLETIEEAGFSPVRMPHLLRLARLDALSDPQMVNELK
                     THMTRASAPAPSIETILHAILPHKYVDHTHADAVITVTNTDNGEARIREIYGDSEVVV
                     PYVMPGFDLARVCA"
BASE COUNT         1749 a         2532 c         2427 g         1905 t
ORIGIN      
        1 ccgactgccg cgggaataat cgcgcctttg gtggggatca cgctgccgac ggttgcgcct
       61 ttgcccaaat gcacatcggg catcaccgcg atgtgcccgg cgagcaccgg cagcttcgcc
      121 atgttttcaa gctggcgcaa ggcttccgct tcgacgggga cgccttcggt ccacatccgg
      181 atttgcgccg cgggttgcat ggcgggacta cgcccggccg acccagagtc tgctgttttt
      241 tggaacgtgg gcgagaagat aatccatctg gtcggcgaga atatgacggt tcgccagaat
      301 catgccctca tagcgattgg gcgcgtaagg cacgtaaagc aatttcatcc ccgcctcttc
      361 cggcgtgcgg ttggacttga cttcgttgca catacggcag gcggtgacca cattggtcca
      421 gtgctccacg ccgccgcgcg aagtcggcgt cacgtggtcc ttggtgagtt cgtgtgcgtg
      481 gtacaggccg ccgcaatagg cgcacatgtg ccggtcgcgg cggaacagcg tttcgcgatt
      541 cagccacggc tcgcattcga aatgtccggt gtgcactttg ccttcgaccg cgatgatggg
      601 acgcggcgcg atttccgaac gtttgccggt gagccgtgaa atgccgccgt ggtacacgca
      661 caccggatcg ccgatggacc aggtcaccag ttcgcgcgcg taatacagca ccgcctcctg
      721 cgtgtcgatc cagcgacgcg gaaggccggc gacatcaagc gtcaatacca gcggcgtgct
      781 catgtgttta atcccgttta gcccgtttgc gggcggcgca taatgccaaa ttaacgcgcc
      841 tgtccgcaga tttacccatt gtatcgcctg cgaagggtat tttccaataa tttagcgact
      901 tagtgataat actaaatttt taacttaact tatcccgctg ggccagcaag ggggtgaata
      961 cggttccact gctggaaaaa ttttcccgcc ggtcggcgaa ttttcggctg ataccctgga
     1021 aaccaaatac tcgatgcacg aactcagaag aggcttactc agagacaatc atcaataatt
     1081 catgaattcg ttgattttta tatgattttt attgtacgcg gtcgccactg catgtttatt
     1141 gcggtggtgc tgcgaaattt atcgcggcat cgcaaggatg aaaattgttt cgcagagttt
     1201 attctgggct catcgcaaaa ctcgcaacga gcggtgattt gttctactcg cgggattttt
     1261 gctggggctc gtccaggatc tgaaaaaaga tttcgttctg cttgatcatg ccaagttcgg
     1321 agcgcgcacg ctcctcaatg gcttcaaaac cctgcttgag atcgcgcacc tcggcgtcca
     1381 gcgcgccgtt gcgcgcctgc agcccccgat tggtggcacg ttgctccttg agctgccggt
     1441 cgatttccca caccctgagc cagcttccct tgccgagcca cagcggatat tgaatcgcag
     1501 cgatcagggc aatcaggatc agagtgaaga atttcatcgg cgcagctggt aaaaagcgcc
     1561 gcgaccggca tagaccgcgc tgtcgccgag gtcctcctca atccgcagca actggttgta
     1621 cttggcgagc cggtccgaac gcgacagcga gccggtcttg atttgcatgg cgttggtcgc
     1681 caccgcgacg tcggcaatcg tggtatcttc ggtttcgccc gagcggtggg agataaccac
     1741 ggtgtaaccg gagtgcttgg cgagctcgat cgccgcgaag gtctcggtga gcgtgccaat
     1801 ctgattgatt ttaatcaata ccgaattggc gatttcctgc ttgatgcctt cggcaaggat
     1861 tttggtgttg gtcacgaaca cgtcgtcgcc gaccagctgc accgaagtgc ccagccggcg
     1921 ggtcagcgtc cgccaaccgt cccagtcggt ttccgccatg ccgtcctcga tgctgatgat
     1981 gggatatttg tcggtccagg cggcaaggta atcgctgaac tgcttggagt tcaagtttaa
     2041 cttttccgat tccaaccgat acttgccctc attgaaaaat tcggaactcg cgcaatccag
     2101 accgatcgcc acatccgcgc ccggctgata gcccgctttc tcgatggctt cgacgatgag
     2161 cttaagcgcc gcctcgttgt ttggaagact tggcgcaaag ccgccttcgt cgcccaccgt
     2221 ggtcggcatg cctttttcgt ctatgagttt tttcagcgta tggaacacct cggcgccgta
     2281 gcgcagcgct tcgcggaagc ttttcgcgcc gagcggaatc agcatgaatt cctgcagatc
     2341 gatgttgttg ttggcgtgcg ccccgccgtt aatcacgttc atcagcggca ccggcatctg
     2401 catctgcccc gacccgccga aatagcggta aagcggcaac cccgcttcct ccgccgccgc
     2461 cttggccacc gccaccgaaa ccgcgagtat cgcgtttgcc cccaggcggg acttgttttc
     2521 cgtgccgtct agatcaatca gcgtgcggtc aatgaagctt tgctcggtgg cgtccagccc
     2581 gatgatggcc tcgcagattt cggtgttgat gtgctccacc gctttcagca cacccttgcc
     2641 gaagtaacgc ttcttgtcgc cgtcgcgcag ctccatcgct tccctggttc cggtggaagc
     2701 ccccgatggc acggcggcgc gccccagcac gccggattcc agcagcacat cggcttccac
     2761 cgtgggattc ccgcgcgaat cgagtatctc ccttgctacc acatcgacga tggcgctcat
     2821 tatttgcttg gcctcgttgg tttgattgtt ttctcggtta acgcctgttc ggcaaagcca
     2881 cggcgcttca ccagctcgtc cagcgcttgc agcgtttgca gcagttcccg catgtatttc
     2941 agcggccagg cgttggggcc gtcagacaag gcctggtccg gatccggatg cgtctccatg
     3001 aaaatcccgg caatgccgct tgccaccgcc gcccgcgcca acaccggcac gaattcgcgt
     3061 tgcccgccgc ttttattgcc ttgtccgccc ggcaattgca ccgaatgcgt cgcatcgaaa
     3121 accaccgggc agccggtctc gcgcatgatc atgagcgagc gcatgtcgga aaccagatta
     3181 ttgtaaccaa acgacacgcc gcgctcgcac accatgatat tgtctttgcc actcgcctct
     3241 ctggctttgt ccaccacgtt cttcatttcg ccgggagaaa ggaattggcc ttttttaata
     3301 ttgaccggct tgcccgtcgc agccacggcg tggatgaaat cggtttggcg gcatagaaac
     3361 gcgggggttt gcagcacatc aaccacggcg gcaacctgcg ccacatcggc ggcttcatgg
     3421 acatcggtta aaaccgggac tttgatctgg tttttaacat catctagaat cttaagacca
     3481 ttatctatcc ctatgcctct aaaggatttt cctgagctgc gattggcttt gtcgtaagac
     3541 gatttaaaga tgaatggcac gcctatttcc ctgcagattt ccttgagcgc accggcggta
     3601 tcgagagcca actgccgcga ttcaacaaca cacgggccgg ctatcaaaaa gagtggttta
     3661 tccagaccgg cgtcgaagcc gcagagcttc atcgagccac gctcagctgt ttggcgcctt
     3721 cgctctcagt aaacgcgccg gagtgacgca atgccgcctc gatgaaggcc gtgaacaagg
     3781 ggtggccggc acggggggta gacgtgaatt cggggtgaaa ctggcaggcg acaaaccagg
     3841 gatgatcggg aagctctatc atctcgcaca actgctcggc cttggtgcgc gccgcgattt
     3901 tcagcccggc agcttcgagc ttgggcagat agaaattgtt cacttcatag cggtggcggt
     3961 gccgttcgct gacaatgttg ctattgtaaa tacgctgcac cagggtgtcg gaaatgatat
     4021 ccgcggtctg cgcgcccagg cgcatggtac cgccgagatc agaatgggcg tcgcggcgct
     4081 cggctcttcc atcgcgattc tgccattccg taaccagggc tatcaccggc tgcgccgtat
     4141 cgggctcgaa ttcggtgctg ttggcctgag tgagccccgc tttgtgccgc gcgtactcga
     4201 tgaccgccag ttgcatgcca agacagatgc ccagataggg aattttattt tcccgcgcgt
     4261 aacggatcgc tttgatcttg ccttccacgc cgcgtttgcc aaagccgccg ggaaccagca
     4321 cagcgtccac ttttttcagg caatcgatgc catctttttc gattgcctcg gaatccacat
     4381 attggatttt cactttggag cgggtgtgga tgccggcatg cactagggct tcgttgagcg
     4441 acttgtagga atcggtgagg tccacgtatt tgccgaccat ggcgatgctg acttcgtgct
     4501 gcggatgttc cagcgcgtcg atcaggcgtt tccagatctt gaggtcggcc ggcttggcga
     4561 ggatttgcag cttgtggcag acgatttcat ccagtatctg ctggtgcagc atgtaaggaa
     4621 tcttgtagat ggaatccacg tcccacaccg aaatcaccgc gttgggcgcc aggttggtaa
     4681 agagcgctat tttcttgcgt tcgtcctcgg gtatcggccg atcggagcga catagcagcg
     4741 catcggcctg gatgccgatt tcgcgcagtt ctttcaccga atgctgcgtc ggcttggtct
     4801 tcatctcccc cgccgacttg atgaaaggca ccagcgtcaa atgcacgtag caggtgttgt
     4861 cgcgccccag ctccagcccc aattggcgga tggcctcaag gaacggcagc gactcgatat
     4921 cgcccaccgt gccgccgacc tcaacaatgg cgacttccgc gccctgcgcg cccttggcga
     4981 tgaaggattt tatctcgtcg gtgatgtgcg gaatcacctg cacggtgcgg cccagatatt
     5041 cgccgcggcg ctccttttta atcacgctct cgtaaatctg gccggcggta aaattgttgc
     5101 gcttgctcat cttggcgttg acgaagcgct cgtagtggcc cagatcgaga tcggtttccg
     5161 cgccgtcctc ggtcacgaac acctctccgt gctggaacgg gctcatggtg ccggggtcaa
     5221 cgttgatgta cgggtcgagc ttgaggaggg tgactttgat gccgcgggat tcgaggatgg
     5281 ctgcgaggga ggcggcggcg atccctttac ccagggagga gaccacgccg ccagttacaa
     5341 aaatatactt ggtcatgaaa tggccagtaa cagtagcaaa tatgataacg caaaaagggt
     5401 cgcgtcacaa tgaaagagaa agctccccgc aagcggcaga cccgctagaa taacagctgc
     5461 caaatctgaa aaagcggaaa ccctcttcat ctatgcacgc catatgggaa acccatctgc
     5521 gcaacaccgg cgccaatctg accgatagta tagtccgtga tttcggcgac ctgcgcgccg
     5581 agctgcgcgc cgcgcgggac ggcacagtcc tatgcgacct ttcgcaatac ggcctactcc
     5641 tgtttgcggg agaagaggcc caggcttttt tgcacgccca gttttccagc gacgtcaaaa
     5701 aaattcatct tgccgccgcc caatacagca gctactgcac ggccaaggga cgcatgctgg
     5761 cgaattttct gctctggcaa tccaacgaag gtttcttcgc ccagcttcat gccggtttgc
     5821 tcgaatctat tcaaaaccgt cttaccaaat tcgtgctgcg caccaaagtg aaaatcagca
     5881 acgtcagcga cagccatgtg cgtctgggac tcgccggcat tcaggccgaa gcattggtga
     5941 aaaaacacct cggcagcctg cccccggcca ggctgggcgt ggctcacgag aaggccgtga
     6001 ccttgattcg cctgtccccc aatcgtttcg aaataattgt cccgcaccaa gaagcgccaa
     6061 aactgtggga tgctctgcgt accgatgcaa aaccggcggg cagcgcctgc tgggactggc
     6121 tcaacgtccg cgacggcgtt ccggtgatta cagcggcaac ccaagagcaa ttcgtgccgc
     6181 aaatggcgaa cctggaactt ctcggcgcgg tgagcttcaa caagggttgc tatccggggc
     6241 aggaaattgt cgcgcgcacc cagcaccggg gtctgctcaa gcggcgcatg tatttggctc
     6301 acgtggacat tgaagcttcg ccgcaaccgg gcgatgagct tttcagccag gatatcgaaa
     6361 gccaggcgag cggaatcata gtgaacgccg cagcggcgcc tgaaggcggt tatgatgtgc
     6421 tggccgtcgt gcaaatcagc agtgcgcaaa gtgacgaggt acgctggaaa tccatgggcg
     6481 gcgccgcttt gaaattgctg cccctgccct attaattttc gcgcataccc tcgaggttcc
     6541 cttaatgcct ctcaattttt acatttatta ccgggtggag caggcccatg ctgcgtcgct
     6601 ggaacccctt attcacgcca tgcaagccgt tttgtggaaa cagaccgggg ttgccggacg
     6661 cctgctgaag aaacgcgatg aaccgctgct gtggatggaa atctacgaaa atgtcgccga
     6721 agccggcccg tttgaagatg cgctgatgca tgcggtcgaa aaataccagg tgaaacaatt
     6781 cgtggaagca ggatcgcagc ggcataccga gtgcttcgtt atttaattac atgtgcctcg
     6841 tcttgctggc ctataaactg tcgccggact ggcctttgct gctcgccgcc aatcgcgatg
     6901 aattctacga gcgctccacc gccgcggcgg cgttttggga agaccatccg gaggttctcg
     6961 gcggccgcga cttgcagaag cacggaacct ggcttggcgt cacgcgcagc gggcgttttg
     7021 ccgccgtcac caattttcgc caggggtttg cccagaagaa aaatgcgccg tcccgcggtg
     7081 cgctggtgag caattatctg ctgggaaaag aaaaaccggg ggactattta cagcggttgc
     7141 aacagcatgc cgccgagtac gatggcttta acctgatagt cggggacatc aaagaggttt
     7201 actactgttc gaaccgcggc ggaaaaatgc aaaaactcgc tcccgggttg tatggcttga
     7261 gcaatcatct gctggatacg ccctggccca aggtggagcg cggcaagaaa cagctcatcc
     7321 ggcttttttc ggcgccgcag ccgccttccg tcgaggcgct gtttgccatt ctggccgacc
     7381 gttttgttcc ggaagataaa gagctgccga acaccggcat cgggccggaa tgggaacggc
     7441 ggttgtcatc ggcttttacc gctaccgccg agtacggcac gcgttcctcc accctgctct
     7501 ttatccattc ggacaaccgg gtcgagctta ttgagcgctc cttcaagcgg gccggcgccg
     7561 agggcggtac cgtggagcac gagttcttaa tcgactctac gcctttataa atgcagaacc
     7621 atttttacat gcggtatgcc tgcgtccatg aattccccgc tcgccacctt gaaaccacgg
     7681 cgggaataaa atgcgacggc gcgggtctgc gaattgagga agacctggct gaatccccgg
     7741 cttctagcga tttgcaaaag agcctcaagc agcgcgctgc ccacgcctaa cccccgccac
     7801 tctttcaata ccgccatgcg gccgatttgg ccgtcgggga gcaggcgtgc cgtgcccatg
     7861 gctacgccgc cgcggctttg cgccaaaaca tgacgtgcgt tcgggtccag cccgtcccat
     7921 tccagttcga ccggaacgtt ctgctcgacg atgaaaaccg cctcgcggac gcgcttgagc
     7981 tcatgctgcg cttcttgcca gtccacttcg cgtatccggt aatcttgcat tttttacgga
     8041 atccccatcc ggccaacgat ataatattgc aactggcaat gagataacca tgaaaagctt
     8101 gtggaacaac aaggaagccg cgcagcacaa gggcgatttg ggcttgcgcg tttatacttc
     8161 gcgcctgctc gggcgcgaca aatcgctggt gctgcacggc ggcggcaaca cttcggtcaa
     8221 aatcaaggag cgcaacatcc tcggcgcgga agaacaaatc ctctacgtca agggcagcgg
     8281 ctgggacctg gaaaccatag aagaagccgg tttctccccg gtgcgcatgc cgcacttgct
     8341 gcggctcgcc cggcttgacg cgctgtccga cccgcaaatg gtgaacgagc tgaaaacgca
     8401 catgacacgc gcttctgccc ccgcaccctc catcgaaacc atcctgcacg cgattctccc
     8461 ccataaatat gtcgatcaca cccacgccga tgcggtgatt accgtgacca ataccgataa
     8521 cggcgaggcg cgcatacggg aaatctacgg cgacagcgag gtggtggttc cttatgtcat
     8581 gcccggtttt gacctggcgc gggtctgcgc cag
//
LOCUS       DATIOW010000034         3495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_523399, whole genome shotgun sequence.
ACCESSION   DATIOW010000034 DATIOW010000000
VERSION     DATIOW010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523399"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VLL03_00970"
     CDS             complement(<1..375)
                     /locus_tag="VLL03_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004553866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HSS45975.1"
                     /translation="MFNPSREEARRFFFNTWHKYKEQGPLTDLEKMALKIMILHPEYH
                     KTLDEPERSLERDYLPEMGAVNPFLHMGMHLAIEEQLSIDQPYGIRTQFERILQKTTD
                     RHEAFHIVMECLGEMIWHAQRCH"
     gene            complement(464..1102)
                     /gene="nth"
                     /locus_tag="VLL03_00975"
     CDS             complement(464..1102)
                     /gene="nth"
                     /locus_tag="VLL03_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HSS45976.1"
                     /translation="MNAAKRREIFTRLKTVNPKPATELEYQTSFELLIAVVLSAQATD
                     KSVNLATRKLFAAANTPQQIFALRQSGLQKYIKSIGLYRTKAKNILAACKILIEEHGG
                     EVPRTREALERLPGVGRKTANVILNTAFGQPTIAVDTHIFRVANRTGVAPGKNVLEVE
                     ERLLKLVPDEFRHDAHHWLILHGRYVCKARKPDCPLCLIRDLCEYKAKTATA"
     gene            complement(1099..1710)
                     /gene="rsxB"
                     /locus_tag="VLL03_00980"
     CDS             complement(1099..1710)
                     /gene="rsxB"
                     /locus_tag="VLL03_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HSS45977.1"
                     /translation="MTASLALVEQIDVILPQTQCGKCGYGGCRPYAEAIAAGEADINQ
                     CPPGGAAGIRRLAALLNREFKPLNPQFGVEKPLEAAVIEEELCIGCTKCIEACPVDAI
                     IGASKQMHTVITAYCTGCELCIPPCPVDCIRMIPAAHPVWSQQDADAARERFNFHNFR
                     LQRDEQEIQQKLAQKTQPEHEPKKAAIRAALERARMRRISSQK"
     gene            complement(1777..3357)
                     /locus_tag="VLL03_00985"
     CDS             complement(1777..3357)
                     /locus_tag="VLL03_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS45978.1"
                     /translation="MLQRWGGVTIAYRRRLIDSPSYTLNHEEVEKALQEGIRFAEGLN
                     PLRVEVDSFGAARAIRMSVQSRDAAGHWSEVNQVELPAHTILVAAGTQPNTVLAREDS
                     GNFKLDGRYFQACDEDGNPVKPEKNISKPKTANVLLSKRSDGRFISFFGDLHPSYYGN
                     VVKALGGAKQGYPVVSGVLERVKSASDKSDAEFFAHLNNELRATVHEVIRLTPSIVEV
                     VVKAPQAARRFRPGQFYRLQNYETNAIKVDGTQLTMEGLALTGAWVDTRKGLISTIVL
                     EMGGSADLCMLLEPGEPVVLMGPTGSPTEIPSDETVILAGGGLGNAVLFSIGQALRRA
                     GSKVLYFAGYKKMIDRYKVEQIEAAADMVVWCCDEHPGFQPDRPQDRAFVGNIVQGIT
                     AYARGALGPQQIAVLDADRIITIGSDKMMAAVAEARHTVLKSYLKPHHFAIGSINSPM
                     QCMMKEICSQCLQPQVNPETGKITYVFSCFNQDQPLDQVDFEGLSARLSQNSLQEKLT
                     AQWIDRCLVRLQRRQAAA"
BASE COUNT          659 a          992 c         1026 g          818 t
ORIGIN      
        1 gtggcagcgc tgcgcgtgcc agatcatttc acccaggcac tccatgacga tgtggaaggc
       61 ttcgtgccga tccgtggtct tttgtagaat ccgctcgaat tgcgttcgta tgccgtaagg
      121 ctggtcaata gaaagctgtt cttcgatggc gaggtgcatg cccatgtgca ggaaagggtt
      181 cacggcgccc atttccggaa gataatcccg ctctagagaa cgctccggct catccaatgt
      241 cttgtgatat tccggatgca agatcattat cttcaacgcc attttttcaa gatccgtgag
      301 cggcccctgc tctttgtatt tgtgccaggt attgaaaaag aagcgccgcg cttcttcgcg
      361 ggaagggtta aacatgaaaa atcagtgaag aagtgaatga agtgaaagag tgaacaagtt
      421 taacacgtgc tcttgtttcg ttccttcacc ccatttactt cgttcacgct gttgctgttt
      481 ttgccttgta ctcgcacaaa tcgcgaatca ggcatagtgg gcaatcgggc ttgcgcgcct
      541 tgcacacata gcgcccgtgc agaatcagcc agtgatgggc gtcgtggcgg aattcatccg
      601 gcacgagctt taacagcctt tcttccacct cgagcacgtt tttccccggc gcgacgccgg
      661 tgcggttggc gacgcgaaag atatgcgtgt ccaccgcgat ggtcggctgg ccaaacgccg
      721 tgttgagaat cacattggcg gtttttctcc cgaccccggg gagtctttcc agcgcctcgc
      781 gggtgcgcgg cacttcacca ccatgttctt caatcagtat tttgcaagcg gcgaggatgt
      841 ttttagcttt ggtgcggtag aggccaatgc ttttgatgta tttctgcagg cctgattgcc
      901 tcaaggcgaa aatctgttgt ggcgtattgg ccgcagcaaa taatttgcgg gtggcaaggt
      961 ttacactctt gtcggtcgcc tgcgccgaga gcaccactgc gatcaaaagc tcgaaagacg
     1021 tttggtattc cagctcggtg gcgggcttgg gattgacggt tttcagccgg gtgaaaatct
     1081 cccggcgctt ggctgcattc atttttgcga agatattctg cgcattcgcg cgcgctccag
     1141 cgctgcccgg atcgcggctt ttttgggctc atgttccggc tgggtttttt gtgccagctt
     1201 ttgctgtatt tcctgttcat cccgctgcag gcggaagttg tggaaattga agcgctcgcg
     1261 cgccgcatcg gcatcctgtt gcgaccaaac cggatgagcg gcgggaatca tgcgtatgca
     1321 atccacgggg cagggcggga tacacagctc gcagccggta cagtaagcgg tgattaccgt
     1381 gtgcatttgc ttggaggcgc cgatgattgc atccaccgga caggcttcga tgcacttggt
     1441 gcagccgatg cagagttctt cttcaatgac cgcggcttct agcggttttt ccacgccgaa
     1501 ttgaggattg agcggcttga attcgcggtt gaggagggct gcgagcctgc ggatgcccgc
     1561 tgctcctccg ggcgggcatt ggttgatgtc ggcttcgccc gcggcaatcg cttcggcata
     1621 gggcctacag ccgccataac cgcatttccc gcactgggtt tgcggcaaaa tcacatcaat
     1681 ttgctcgacg agggcaagac ttgcagtcat cccgcaattc tactgcaaaa aacagagtgt
     1741 tcgtgctgaa cggggtaagc tggagttgcc gctgttttaa gccgcggctt gccggcgttg
     1801 caggcgcacc aggcagcggt ctatccactg cgcagtgagc ttctcctgca gcgaattttg
     1861 gctcaggcgc gcggagagcc cctcgaaatc cacttggtcg agcggctggt cctggttgaa
     1921 gcaggaaaag acgtaggtaa tcttgccggt ttccggatta acttgcggct gcaggcactg
     1981 cgaacagatt tccttcatca tgcattgcat cggcgagttg attgagccga tggcgaagtg
     2041 gtgcggcttg aggtaagact tcagcaccgt atggcgggct tcggccaccg ccgccatcat
     2101 cttgtccgag ccgatggtga taatgcggtc ggcgtcgagt acagcaatct gctgcggccc
     2161 cagcgcgccc ctggcgtagg cggtgatgcc ctgcacgatg ttgccgacaa aagcgcggtc
     2221 ctgcggccga tccggctgga aaccgggatg ttcgtcgcag caccacacca ccatatctgc
     2281 ggcggcttcg atttgttcta ccttgtagcg gtctatcatt tttttgtagc cggcgaaata
     2341 cagcaccttg gaaccggcgc ggcgcaacgc ctggccgatg gagaaaagca ccgcattgcc
     2401 caggccgccg ccggctagaa ttaccgtctc atccgaagga atctcagtgg gtgagccggt
     2461 cgggcccatc agaaccaccg gttctccggg ttcgagcagc atgcacagat ccgccgatcc
     2521 gcccatttcc agaacgatgg tggaaatcaa acctttcctg gtatccaccc acgcgccggt
     2581 gagcgccaag ccttccattg tgagctgtgt gccgtctact ttaattgcgt tggtttcgta
     2641 attctgcagc cggtagaact gaccgggacg gaagcggcgc gccgcctgcg gcgctttgac
     2701 cacgacttcg acgatgctag gcgtgaggcg gattacctcg tgcaccgtgg cacgcagttc
     2761 attattgagg tgtgcaaaga attctgcgtc gcttttgtcg ctggccgatt ttacgcgctc
     2821 cagcacgccg ctcaccaccg ggtagccttg cttcgccccg cccagcgctt tcactacatt
     2881 gccataatag gaaggatgca gatctccgaa gaagctgata aagcgcccgt cactgcgctt
     2941 cgacaacagc acgttcgcgg tcttcggctt ggaaatgttt ttctccggct tgaccggatt
     3001 gccgtcttcg tcacaggcct ggaagtagcg gccgtccagt ttgaaattcc ccgaatcctc
     3061 gcgcgccaga acagtattag gctgtgttcc cgccgcgacc agaatggtgt gtgcgggaag
     3121 ttcgacttgg ttgacttcgc tccagtgacc tgcggcgtct ctgctctgta ccgacatgcg
     3181 gatggcgcgc gccgcgccaa atgaatccac ctccacgcgc agcgggttca agccttcggc
     3241 aaagcggatg ccttcctgca gcgctttttc cacttcctcg tggttgagcg tgtaggaggg
     3301 actgtcgatc agccgtcggc ggtaggcgat cgtcacgccg ccccagcgct gaagcatctc
     3361 ggcgatccgc ggcgagcgcc aggaggcggc acgcgagcat cgcgcgccgc gcatcgccga
     3421 gatgcttcag cactggggcg gcgtgacgat cgcctaccgc cgacggctga tcgacagccc
     3481 ttcctacacg ctcaa
//
LOCUS       DATIOW010000035         6487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_828007, whole genome shotgun sequence.
ACCESSION   DATIOW010000035 DATIOW010000000
VERSION     DATIOW010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828007"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..827
                     /locus_tag="VLL03_00990"
     CDS             303..827
                     /locus_tag="VLL03_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HSS45979.1"
                     /translation="MAEETGSYHNRLATLEHLGEGTVVAEQIFGMVGHSPFFAELDQE
                     DVRVLAGFMQVYRAQSGEVIIREGDPGDYMLLLIQGQVDILKAGQHRTERHMTTVLPG
                     MTLGEMSMIDGEPRFATCIAKDTATFAVLTRDDMAKIILEQPSLGAKILIKLVTLLSQ
                     RLRQTSHKLLDHME"
     gene            856..1734
                     /locus_tag="VLL03_00995"
     CDS             856..1734
                     /locus_tag="VLL03_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HSS45980.1"
                     /translation="MNFFLFFPACAIFFSTLIFLTAFASPVRVTDDRGGTVVLDQPAQ
                     RIIALAPYITELVYAAGAGSKLVGASEYSDYPEAAKNITRVGDAAHIDIERILLLKPD
                     LILGWRSGSHAGELARLEKLGFKVFVMEPVKLADIPRLLRALGKFAGTRAAAEIAAKD
                     FEDRLEALRKRFSGKRPVTVFYEIWHEPLMTVNGEHIISDVLKLCGGVNVYANVRPLT
                     PIISIEGLLAEDPEAIISSVSQGELWQPFKVLSAVRKERIFFIPPEWLERQSPRILEG
                     AREICERLDTVRKEKR"
     gene            complement(1738..2427)
                     /locus_tag="VLL03_01000"
     CDS             complement(1738..2427)
                     /locus_tag="VLL03_01000"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HSS45981.1"
                     /translation="MNYTNLELREKLAAEYALGTLRGAARARFTRLMKYDPALRRTVV
                     EWENRIYPILEAAPEIAPPARVWNAIKARIAGNAGAPVNLGFWRTLGLFSSGLAAVLL
                     IYLSIASRPEAPPAYMALLNDQKTQQPVLLVSTKRNSPELTVKALITQPIAADKTLEL
                     WALPAEQGMAPKSLGLVAASGVTRLMLQQPADQQLGNVPALAISLEPKDGSPTGLPTG
                     PVLYVGKWLRL"
     gene            complement(2424..2927)
                     /locus_tag="VLL03_01005"
     CDS             complement(2424..2927)
                     /locus_tag="VLL03_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSS45982.1"
                     /translation="MGDQAAFANLYRQTSAKLFGVALRILKREEWAEEVLQDSFVNIW
                     KHAAEYTAPKSAPMTWMTSIVRNRALDLLRRPGLEVAQEDNSLIEDFGDGAQGPVELL
                     LQSNAADALARCMQQLEARQRQSIMLAFYHGLTHAELAKHLRQPLGTVKTWIRRGLER
                     LKRCLSA"
     gene            complement(2994..3539)
                     /locus_tag="VLL03_01010"
     CDS             complement(2994..3539)
                     /locus_tag="VLL03_01010"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HSS45983.1"
                     /translation="MGHRLTKIYTRTGDNGTTGLGDGSRTPKDSPRIEAIGEIDELNS
                     CIGVLLAENLPPRLRACLTDIQHDLFDLGGELCMPGHSSLTPKQVERLENTLDALNAD
                     LPPLKEFILPGGSRAASLAHLARTVCRRAERSLVKLAGSEKISTASCHYLNRLSDLLF
                     VTGRVLNRKHGDVLWQQGKNK"
     gene            complement(3602..5359)
                     /locus_tag="VLL03_01015"
     CDS             complement(3602..5359)
                     /locus_tag="VLL03_01015"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HSS45984.1"
                     /translation="MHYVLGLLSFLFALCAQAAQHDLIQWQPWSDGLFEQAKRENKFI
                     LLDLEAVWCHWCHVMDRETYGNPEVAKLIRAKYIPVKVDQDARPDISRRYEDYGWPAT
                     IVFNSDGGEIVKRSGYLPPPNMLAMLKEIINDPSPIIYPDVRILNVSDFSNQTLLDDK
                     LRSELKQRFFLSHDYRVGGLDQQQKFMDRDSVEYAMLLTKKGDAKAGTMARQTLDGAL
                     NLIDPIWGGAYQYSTDHDWKHPHFEKIMQVQAEYMRVYSLAYLHFRDPRYLRAGEDIY
                     RYLKDFLTSPEGAFYTSQDADLVKGEHSGEYFALDDTGRRKLGLPAIDKHRYARENGW
                     AINGLAHLYAASGDEQYLAHAIKAANWIIQNRSLPNGGFRHDKVDSAGPYLEDTLAMG
                     RAFISLYTVTADRQWLQHAERAADFIDKNFKAQAGFATSAAKGVLKPRPTIEENIFTA
                     RFANLVYQYSGREPYRKLTEHAMRFLATKNIALYRRTEAGILLADNELANEPSHITVV
                     GHKDDPQAQALYRSALTYPSVYRRIEWWDTREGKMPNPDVKYPELPTAAAFVCSNKRC
                     SLPLFKPGEIALAADRLNQ"
     gene            5673..5963
                     /locus_tag="VLL03_01020"
     CDS             5673..5963
                     /locus_tag="VLL03_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSS45985.1"
                     /translation="MIVAIPVSAQAAGDPAQGQKKNSMCAGCHGIPDWRTAYPEVYSV
                     PKLGGQHAAYIVKALQAYKSGARNHPTMKAIAGSLTDQDMADLAAYYSGGGK"
     gene            5991..6317
                     /locus_tag="VLL03_01025"
     CDS             5991..6317
                     /locus_tag="VLL03_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSS45986.1"
                     /translation="MNKLVTVATASALLLAAGHSFAGGDAAAGKQKSAACAACHGADG
                     NSATPDFPRIAGQQPDYIVKALSDYKSGARKDPIMGAMGANLSKQDMEDLAAYFSSQS
                     GLVVKY"
BASE COUNT         1315 a         1767 c         1987 g         1418 t
ORIGIN      
        1 aagcctacca aaagcgagtg gcggccgccg cgcgttatac aaattgagag gggagagtgg
       61 cgggcccggt ttccacgtat tgaattggcg ttgcggcgtt gaaatatatc ttgtatgact
      121 gggcgggctt gtctagaatc agcccgggcg ctcattttcc cgcggacgtg ccgaccggct
      181 aggtgaaatc catgctgccg gtgtgctaca tcgttacacg ggttttcaat aggtattcag
      241 gcaatacagc atgagcgcgc gagcgctggt aaaataggca aaacgcgaag aagggcgcag
      301 tcatggcgga agaaacaggt tcttaccata accggcttgc gacgctggaa catctcgggg
      361 aagggacagt ggtcgcggaa cagatcttcg gaatggtcgg ccattcgccg tttttcgccg
      421 aactggacca agaagacgtc agggtgctgg cgggtttcat gcaggtgtac cgggcccagt
      481 ctggagaggt tattattcgt gaaggagacc ctggcgacta catgctgctg ctgatccagg
      541 gacaggtgga tattctcaag gcaggccagc acagaactga gcggcacatg actacagtgc
      601 tgccgggaat gactctgggc gaaatgtcga tgatagacgg ggagccgcgc tttgctacct
      661 gtatcgcgaa ggacaccgct acttttgccg tgctgacccg ggatgacatg gcgaaaatca
      721 ttcttgagca accaagcctc ggcgcaaaaa tcctgatcaa gctggtcacc cttttgtctc
      781 aacgcctgcg ccagaccagc cataagcttc tggatcacat ggagtgataa gcttgttggt
      841 gttccggttt ttcgcttgaa tttcttttta ttcttccctg cctgcgcgat atttttttcc
      901 acgctcatct tcctgaccgc ttttgcttcc ccagtccggg taacggatga ccgtggcggg
      961 acggtagtgc tcgatcagcc ggcacagcgg attatcgcgc ttgcgcctta tattaccgag
     1021 ctggtttatg cggcgggagc ggggtcgaaa ctcgtcggag cgagcgagta cagcgattat
     1081 cccgaagcgg cgaaaaacat cacgcgggtt ggagatgcgg cgcatatcga tattgaacgc
     1141 atcctgctcc ttaagcctga tttgattctc ggctggcgca gcggcagcca cgcgggggag
     1201 ctagccaggc tggaaaaact cggcttcaag gtgttcgtca tggagccggt caagctcgct
     1261 gacatacctc gattgctgcg cgcgctggga aaatttgccg gcacccgggc ggcggcggaa
     1321 atcgcggcga aggattttga agaccggctg gaggccctga gaaaacgctt cagcggcaag
     1381 cgcccggtca ccgtatttta tgaaatctgg cacgagccgc tgatgaccgt caacggcgag
     1441 cacataatca gcgacgtact gaagctatgc ggtggcgtga acgtttatgc caatgtgcgc
     1501 ccgctgactc cgataatttc catcgaaggc ttgctggccg aggatccgga agccattatt
     1561 tcaagcgtat cgcaaggcga gctatggcag ccattcaaag tgttaagcgc tgtgcgcaaa
     1621 gagcgcattt ttttcattcc gccggaatgg cttgaacgcc agtcgccgcg gatattggaa
     1681 ggtgcgagag aaatttgcga gcggctggat acggtgcgaa aagagaaacg ctgaagctta
     1741 cagcctcagc cacttgccca cgtacagcac cggccccgtc ggcaatccgg tgggcgatcc
     1801 gtctttgggt tcgaggctga tggcgagcgc aggcacgttg ccgagttgct gatcagcggg
     1861 ttgttgcagc atgagccggg tgacgccgga ggccgcaacg aggccgagtg atttcggcgc
     1921 catgccttgc tcggcgggca gcgcccacaa ctccagcgtc ttgtcggctg cgatgggttg
     1981 cgtgatgagg gctttgacgg tgagctcggg cgagttgcgc ttggtgctca ccagcagcac
     2041 cggctgctgg gttttctggt cgttgagcag cgccatgtag gcgggcggcg cttccggccg
     2101 cgacgcgatg ctcaggtaaa tgagaaggac cgcggccaaa cctgagctga aaagacccag
     2161 tgtgcgccaa aagcccaagt taacaggcgc tccggcattt cccgcgatcc gcgccttgat
     2221 ggcgttccac acccgcgccg gcggggcgat ttcaggcgcg gcttccaata tcgggtaaat
     2281 gcggttttcc cattcgacga cggtgcggcg cagcgccgga tcgtatttca tcaaccgggt
     2341 aaagcgcgca cgcgccgcgc cgcgcagggt gccgagcgca tattccgcag ccagtttttc
     2401 gcgcaattca aggttcgtgt aattcatgct gaaaggcacc tcttaagccg ttccagaccg
     2461 cgccgaatcc aggttttcac cgtgcccagg ggctgacgca aatgcttcgc cagttcggcg
     2521 tgggtcaggc cgtgataaaa cgcgagcatg atgctctgcc gttgccttgc ttcgagttgc
     2581 tgcatgcagc gtgccagcgc gtccgctgca ttcgattgca acagcagctc caccgggcct
     2641 tgtgcaccgt ccccaaaatc ttcgatcaac gagttgtctt cttgtgcgac ttccaggccg
     2701 ggacggcgca gcaaatccag cgcgcggtta cgcacgatgc tggtcatcca tgtcattggc
     2761 gcgctcttgg gggccgtgta ttcggcggca tgcttccaga tgttcacgaa gctgtcttgc
     2821 aaaacttctt ccgcccactc ctcccgcttt aatatacgta gcgcgacgcc aaacagtttc
     2881 gccgaggtct ggcggtacag attggcgaaa gccgcctggt cgcccaacgc gcattgcgca
     2941 agtagccggc tcagcgcttc cggctggagt ttgggactaa tggagagcct ccgttatttg
     3001 ttctttccct gttgccacag tacgtcgccg tgtttgcggt tcagcacccg tcccgtcaca
     3061 aacaacaggt cggacaagcg attgagataa tggcagctcg ccgttgagat tttttcggag
     3121 ccggcgagct ttaccaggct gcgctcggcg cggcggcaca cggtgcgcgc gagatgcgcc
     3181 aggctcgcgg cgcgcgagcc cccgggcaga atgaattcct tgagcggagg caaatcggcg
     3241 ttgagggcat cgagcgtgtt ttctagccgc tcgacctgct ttggcgtgag actggaatgc
     3301 cccggcatgc acagctcgcc gccgagatcg aacaggtcgt gctggatatc ggtcaggcag
     3361 gcgcgcaggc gcggaggaag gttttcggcg agcagtacgc cgatgcagga attgagttcg
     3421 tcgatttcgc cgatggcttc gatgcgcggg ctgtccttcg gggtgcgcga accgtcaccg
     3481 aggccggtgg tgccgttgtc gccggtacgc gtgtagattt tggtcagccg gtggcccatg
     3541 gtgctccctg cgtttccgtt gagggattat aggcattaag gggtggcctg aacagtgcgg
     3601 cttattggtt caagcgatcg gctgcaagcg cgatttcccc gggtttgaac agcggcagag
     3661 aacagcgttt gttgctgcac acgaaagctg cggcagtcgg cagctccgga tatttgacat
     3721 ccggattggg cattttgcct tcgcgcgtgt cccaccattc aatgcggcgg tacactgaag
     3781 gataggtgag cgcggagcgg tacaaggctt gcgcttgcgg atcgtccttg tggccgacca
     3841 cggtgatgtg agaaggctcg ttggccagct cgttgtcggc gagcaggatg ccggcttcgg
     3901 tgcggcggta cagcgcgatg ttttttgttg cgaggaagcg catcgcgtgc tcggtcagtt
     3961 tgcggtatgg ttcgcggccg gagtattggt aaaccagatt ggcaaagcgt gcggtgaaaa
     4021 tattttcttc gatggtcggg cgcggtttca acacaccttt tgcggcgctg gtcgcaaagc
     4081 ctgcctgggc cttgaaattt ttgtcaataa aatcggcggc ccgctccgcg tgctgcaacc
     4141 actgccggtc ggcggtgacg gtatagagac tgatgaatgc ccgtcccatg gccagcgtgt
     4201 cttcgagata agggccggcg ctatctactt tatcgtggcg gaaaccacca ttgggcagcg
     4261 agcggttttg aattatccag ttcgccgcct tgatggcgtg ggctagatat tgctcgtcgc
     4321 cggaagcggc ataaaggtgt gcaagcccgt taatcgccca gccgttctcg cgcgcataac
     4381 ggtgcttgtc gatcgccggc aggccgagtt tgcgccgtcc cgtgtcgtca agcgcaaagt
     4441 attcgccgct gtgctcgccc ttgacgagat cggcgtcctg gctggtgtaa aaagcgcctt
     4501 ccggactagt cagaaaatcc ttgaggtagc ggtaaatgtc ttcacccgcc ctgagatagc
     4561 gaggatcgcg aaaatgcagg taggccagcg aatacacccg catatattca gcctgcactt
     4621 gcatgatttt ctcgaaatgc gggtgcttcc agtcgtggtc cgtcgaatac tggtacgcgc
     4681 cgccccagat cgggtcgatt agattaagcg ctccgtccaa ggtttgacgc gccattgttc
     4741 cggccttcgc gtcgcctttt ttggtgagca gcatcgcgta ttcgacgctg tcgcggtcca
     4801 tgaacttctg ttgctggtcg agtccgccga cccggtaatc gtgggacaga aagaagcgct
     4861 gctttagttc gctgcgcaac ttgtcatcaa gaagcgtctg gttggagaaa tcgctgacgt
     4921 tcagaatacg gacgtcgggg tagataatcg gactgggatc gttgatgatt tccttaagca
     4981 tcgcgagcat gtttggcggc ggaaggtaac cggagcgctt gacgatttcc ccaccgtccg
     5041 agttaaacac gatggtcgcc ggccagccgt aatcttcgta gcgcctggag atatcggggc
     5101 gggcatcctg atccactttc accgggatgt atttggcccg aatcagcttg gcgacttccg
     5161 gattgccgta ggtttcccga tccatgacgt ggcaccagtg gcaccacacc gcttccagat
     5221 cgagcagaat aaacttgttt tcgcgcttgg cttgttcgaa caggccatcc gaccagggct
     5281 gccactgaat gaggtcgtgt tgcgcggcct gcgcgcaaag cgcaaagaga aaactcagca
     5341 aaccgagaac gtagtgcatg ttcttagacc accttggaaa aagcggcctc ggaaagacta
     5401 accgaggccg caaagctcct tgtaaagggg caatgtcaac ttactgtgga acagtccggt
     5461 ttaagctgaa gtccgtcggg ttccccctaa gacagcggcc tggtccgggc ggttcaatgc
     5521 atgatacggg cgagggccgg atctggatgc cggcgcttgc caagaatcat tgaccttaat
     5581 ccaagtatcc tttaaagttc cgcaatccga tttgttcctt tcaagagcta taagacatga
     5641 aaaaaaccag cctgttcatc ctttgcgccg ccatgattgt tgcgattccc gtttcggcgc
     5701 aagcggcggg cgacccggcg caagggcaaa agaaaaactc gatgtgcgcg ggctgccacg
     5761 gcattccgga ttggcgcacc gcttaccccg aggtttacag cgtgcctaag ctcggcggtc
     5821 agcatgcagc ttatatcgtc aaggcgctgc aagcctacaa gtccggcgca cgcaaccatc
     5881 cgacaatgaa ggcgattgcc ggcagcctga ccgaccagga catggctgat ttggcggctt
     5941 attattcggg cggcggcaaa taagacaatc ggagatacgg gaaaaataga atgaataaac
     6001 tggtcacagt tgccactgca agcgcattgt tactggccgc aggccactct tttgccggcg
     6061 gtgacgctgc agccggcaaa cagaaatccg ctgcatgcgc agcgtgccac ggcgcggacg
     6121 gcaatagtgc cacgcccgac ttcccgcgca tcgccggcca gcagcccgat tatatcgtta
     6181 aggccctttc cgactacaaa tccggtgccc gcaaggatcc catcatgggg gcgatgggcg
     6241 cgaatctttc caaacaggac atggaagacc tggcggcgta tttctccagc cagagtggat
     6301 tggtagtcaa gtattaagtg aagggtgaaa agtgagggtt gtgttgaact ttactcctca
     6361 cgcctgacgc ctcactcctt acgctgtttc cccaggcatt ccaggtaaat tttctcgtca
     6421 ggcgcagtgt ggcagcgctg cgcgtgccag atcgtttcac ccaggcactc catgacgatg
     6481 tggaagg
//
LOCUS       DATIOW010000036         9728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1945334, whole genome shotgun sequence.
ACCESSION   DATIOW010000036 DATIOW010000000
VERSION     DATIOW010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9728
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1945334"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2515
                     /locus_tag="VLL03_01030"
     CDS             <1..2515
                     /locus_tag="VLL03_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HSS45987.1"
                     /translation="NKGILKVMSKMGISTYQSYCGAQIFEAVGLNSAVVQKYFTGTAS
                     QVEGIGILEVAEETVRLHKLAFSDHPLYRNALDPGGEYAFRVRGEEHTWTPESIAKLQ
                     HATRANNLKTYKEYAELINEQSQRLMTLRGLFEIKESPNPIPLEEVEPAKEIVKRFST
                     GAMSLGSISQEAHTTLAIAMNRIKGKSNTGEGGEDPQRYKPITKGGMLADTMTGVLVD
                     IPVQPGDSLRSAIKQVASGRFGVTTEYLVNADQLQIKIAQGAKPGEGGQLPGHKVSPY
                     IAQVRHTVPGVGLISPPPHHDIYSIEDIAQLIHDLKNANPHASVSVKLVSEVGVGTVA
                     AGVCKAKSDYVTIAGHDGGTGASPWSSIKHAGTPWELGLAETQQTLVLNRLRGRITVQ
                     ADGQMKTGRDVVVGALLGADEFGFSTAPLVVEGCIMMRKCHLNTCPVGVATQDAELRK
                     KFTGLPEHVVNYFFFVAEEVREYMAKLGFRTFNEMIGRSDRLDTKKAVDHWKARGLDF
                     SKIFHRPDVPADVARYHCEKQNHGLEKALDHKLIAEAKPALERRQPVAVETTIRNIHR
                     VVGTMLSGEVAKRYGHAGLPDDTIYIKLRGTAGQSFGAFLAHGVTLELEGEGNDYVGK
                     GLSGGRIVIYPPANCPIVPEQNIIVGNVVLYGAISGECYFRGVAGERFAVRNSGAIAV
                     VEGVGDHGCEYMTGGTIVVLGYTGRNFAAGMSGGAAYVLDEAGDFERRCNLAMVELEA
                     VPEEEKALEQLGGGDLEGHGKVNVKQLGQSDERLLRVLIQKHLHYTGSARAKEILDHW
                     DKYLAKFVKVMPTEYRRALKEMAAAESGAQPKAGIAQHG"
     gene            2508..3929
                     /locus_tag="VLL03_01035"
     CDS             2508..3929
                     /locus_tag="VLL03_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HSS45988.1"
                     /translation="MGKTTGFMEIARHDLPALPVSERVHNFREFFLHLPDAEMSKQGA
                     RCMDCGIPFCQTGCPINNIIPDWNDLVYRQNWREALDVLHSTNNFPEFTGRICPAPCE
                     AACTLNINDDPVTIKSIEHAIIDKGWEMGWVVPQIPARKTGKRAAVVGSGPAGLACSQ
                     QLARAGHDVVLFEKNDRIGGLLRYGIPDFKMEKHHIDRRMEQMRTEGVEFRVNCHIGV
                     NYPAKKLLDEFDAVVLTGGSEHPRDLPVPGRDLKGIYFAMEFLPQQNKVNAGDIIPNQ
                     ITAKGKHVVVIGGGDTGSDCIGTSNRQGAASVTNFELMPQPPLQENKPLTWPNWPMKL
                     RTSSSHEEGCDRDFAIATQRFSGKGGHVATLHTVKLEWKAGKMQEIPGTEFEVKADLV
                     LLAMGYLYPVHEGMLQELGVEFDPRGNVKADTNKYQTSISKVFAAGDMRLGQSLVVWA
                     IREGRQAARAVDEFLMGCSDLPR"
     gene            4097..5158
                     /gene="hemE"
                     /locus_tag="VLL03_01040"
     CDS             4097..5158
                     /gene="hemE"
                     /locus_tag="VLL03_01040"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HSS45989.1"
                     /translation="MSKLKNDTLLRALSRLPTPYTPVWLMRQAGRYLPEYNETRKRAG
                     DFLALCKNPDLACEVTLQPLARFNLDAAILFSDILTIPDAMGLGLYFVENEGPKLAKP
                     LRDEREIQKLKTPDPDSHLRYVMDAISQIRKALGGSVPLIGFSGSPFTLASYMIEGGG
                     GPDFPQVKEMLYRRPDLLHHVLAVNTRALTAYLNAQTESGAQAVMIFDTWGGILSSHA
                     YHEFSLRYTEEVLHGLIRERDGMRVPSIVFTKGGGEWLESIAATGCDCVSLDSLTDIG
                     LARRRVGHKVALQGNLDPAALFSSPEIIAKEVEKILESFGAHPGHVFNLGHGVMPTTP
                     PENVAAMVEAVHHLSRNRH"
     gene            5494..7668
                     /locus_tag="VLL03_01045"
     CDS             5494..7668
                     /locus_tag="VLL03_01045"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HSS45990.1"
                     /translation="MSIARVALDVPLDKLFDYLAPDLTREHIGLRVLVPFGKKQIVGV
                     VVDIAEKSNIAPQRLKRIVSVFHDISPLGAQILDVFKFCSDYYHHPLGQVMVNALPAR
                     LRRSRPITRKTSGVFCLTETGRKLDPSALPAQAAVKRKLFERLKSGALKKEELRQISP
                     GAAKALNEFITLGWAHEVAAASSRPPGQPADPHQLTAEQAGVVESIQLAGFSCSLLFG
                     VTGSGKTEIYFNRITRALAEKKQVLLLVPEINLSPQLEAWLKQRFPQSRVVSLHSGLN
                     DGERLENWLAAQSGEAQVILGTRLAVFTPIPKLGFIIVDEEHDASFKQQDGLRYSARD
                     VAIFRAKQADVPVILGSATPALESYQHALSGRYRLAKLAQRAVDNALLPELRCIDIRR
                     LKLNEGLSEPLIGAIRQRLERGEQCLIFLNRRGYAPVLMCTTCNWISGCTRCTAGLVL
                     HLREKKLRCHHCGHEERVPAACPNCGSADLLPLGQGTQRVESALSALFPGARVLRIDR
                     DSTRRKFAWPEMLKRIRDHEVDILVGTQILAKGHDFPKLSLVGILNPDSSLYSTDFRA
                     SERLFAQLMQVAGRAGRAQIPGEVLIQTQFPDHPLYEALQRHDYSAFAKTLLAERRHA
                     GFPPFVHQALLRAEATRSSTVMSYLADAAKLASRISNEVTIYDPVPAAVQRLAGRERG
                     QLLVQSPSRKKLQLFLKEWYAKLAAADNKKVRWALDVDPLEF"
     gene            complement(7791..8024)
                     /locus_tag="VLL03_01050"
     CDS             complement(7791..8024)
                     /locus_tag="VLL03_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS45991.1"
                     /translation="MNAVSMGGILKPQREEPWPGNITLYIDVDDLAAYRKKIIAAGGK
                     IYVEEQEVPGMGAFSLFTDLEGRMMGLWETKKK"
     gene            complement(8168..8527)
                     /locus_tag="VLL03_01055"
     CDS             complement(8168..8527)
                     /locus_tag="VLL03_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HSS45992.1"
                     /translation="MRYVDGFVVPVPKKKLQAYRRMAQMSGKIWREHGALEFCECVAD
                     DVKSGKWTSFPQSVKLKRGETVVFSYIVYKSRAHRDRVNAKAMKDKRLAAMMEAKPWP
                     FDGKRMFFGGFKVMVSS"
     gene            complement(8557..8931)
                     /locus_tag="VLL03_01060"
     CDS             complement(8557..8931)
                     /locus_tag="VLL03_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013094005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HSS45993.1"
                     /translation="MSTNSGPGYKKYPNHRLTTKPAGVRVQVRFMGEVIADSQDAVRV
                     DEGDYPAVYYIPRKDVKMDRLVRSNHQTYCPFKGTASYYSLKNGAEDAVWTYEQPYDE
                     MSVIKERLAFYPDKVDSIVVTE"
     gene            complement(9179..>9728)
                     /locus_tag="VLL03_01065"
     CDS             complement(9179..>9728)
                     /locus_tag="VLL03_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HSS45994.1"
                     /translation="PDYTAGCPSCSAIADGFNGFAVHLANHDVTISAVSRAPLAKLQD
                     YKRRMGWTFPWASWHGGDFNFDFNVSLTAEQQREGGFEYNYRREAAWQLRDDEGIASR
                     MPGTPVADNAAMTGTDVATYTRERPGMSAFVLEHGVVYHTYSTYARGLDGLWGMYQWL
                     DRAPKGRNETGVWWRRHDEYKG"
BASE COUNT         2086 a         2931 c         2757 g         1954 t
ORIGIN      
        1 caacaaaggc attctcaaag taatgtccaa gatgggcatt tccacttacc agtcctattg
       61 cggcgcgcag atttttgagg cggtggggct caactcagcc gttgtgcaga aatatttcac
      121 cggcaccgcc agccaggtcg aaggcatcgg cattctcgaa gtagcggaag aaaccgtgcg
      181 gctgcacaag ctcgccttca gcgaccatcc gctttaccgc aatgcgctcg acccgggcgg
      241 tgaatacgct ttccgcgtgc gcggtgagga gcacacgtgg acgccggaat ccatcgccaa
      301 attgcaacac gcgacccgcg ccaacaatct caagacttat aaggaatacg ccgagcttat
      361 caacgagcaa agccagcggt tgatgacgct gcgcggcctg tttgagatca aggaatcgcc
      421 caatccgatt cctttggaag aagtcgagcc ggcaaaggaa attgtcaagc gcttctccac
      481 cggcgccatg tcgctgggct cgatttcgca ggaagcgcac accacgctgg cgattgccat
      541 gaaccgcatc aagggcaagt cgaacaccgg cgaaggcggc gaggacccgc agcgctacaa
      601 gcccattacc aaaggcggca tgctggccga caccatgaca ggggtgctgg tggacatacc
      661 ggtgcagccg ggcgattcac tgcgctccgc catcaaacag gtggcctccg gccgctttgg
      721 cgtcaccacc gaatatctgg tgaacgccga ccagctgcaa atcaagattg cccaaggcgc
      781 gaagccgggc gaaggcgggc aattgcccgg ccacaaagtg agcccctaca ttgcccaagt
      841 gcgccacacc gtgccgggcg tgggtctgat ttcaccgccg ccgcaccatg acatttattc
      901 catcgaggat atcgcgcagc tcattcatga tctgaaaaac gccaatcccc atgccagcgt
      961 cagcgtcaaa ctggtgtccg aggtcggggt gggaacggtt gccgcggggg tatgcaaggc
     1021 caaatccgat tacgtcacga ttgccggcca cgacggcggc accggcgcgt ccccctggag
     1081 ctcgatcaaa cacgccggca cgccttggga gctgggcttg gccgagaccc agcaaacact
     1141 ggtgctgaac cgcctgcgcg gacgcattac agtgcaggcc gacggccaga tgaagaccgg
     1201 ccgcgacgtg gtggtgggcg cgctcctggg cgcggatgaa ttcggcttct ccacggcgcc
     1261 gctggtagtg gaaggctgca tcatgatgcg caaatgccat ctcaacacct gtccggtggg
     1321 cgtagccacg caagacgcgg aactgcgcaa aaaattcacc ggcctgcctg agcatgtggt
     1381 gaattacttc ttcttcgtcg ccgaggaagt gcgcgagtac atggcgaagc tgggtttccg
     1441 caccttcaac gaaatgatcg gccgctccga caggctggac acgaagaagg cggtcgacca
     1501 ttggaaagcc aggggtctgg atttctcgaa aatcttccac cgacccgacg tgcctgcgga
     1561 cgtggcgaga taccattgcg aaaaacaaaa tcacggcttg gaaaaagcgc tcgaccataa
     1621 actcatcgcc gaagccaagc ctgccctcga gcgccggcaa ccggtggcgg ttgaaacgac
     1681 tatccgcaat atccaccgcg tggtgggcac gatgctctca ggcgaagtgg cgaagcgcta
     1741 tggacatgcg ggcctgccgg acgacacgat ttacatcaag ctcagaggca ccgcgggcca
     1801 gagtttcggc gccttcctcg cgcatggcgt aacgctggaa ctggaaggcg aaggcaacga
     1861 ctacgtgggc aaaggcctct cgggcggacg catcgtgatc tatccgcccg ccaactgccc
     1921 gattgttcca gagcagaaca tcatcgtcgg caacgtagtg ctctacggcg cgatttccgg
     1981 cgaatgctat ttccgcggcg tcgccggcga gcgcttcgcc gtgcgcaact ctggcgcgat
     2041 tgccgtggtg gaaggcgtgg gcgaccacgg ttgcgaatac atgaccggcg gcaccatcgt
     2101 ggtgctgggt tacaccggcc gcaatttcgc cgcgggaatg agcggcggcg cggcctacgt
     2161 gctcgatgaa gcaggcgact tcgagcgccg ctgcaacctc gccatggtcg aactcgaggc
     2221 ggtgccggag gaagaaaaag ccctggaaca attgggcggt ggcgatctgg aaggccacgg
     2281 caaggtcaac gtcaaacaac tcggacagtc cgatgagcgg ctgctgcgcg tcctgattca
     2341 aaagcacctg cattacaccg gcagcgcgcg ggccaaggaa attctcgacc actgggacaa
     2401 gtatcttgcg aaattcgtca aggtgatgcc gaccgaatac cggcgcgcct tgaaggaaat
     2461 ggccgcggcc gaaagcgggg cgcaacccaa ggctggaatc gcgcagcatg ggtaagacca
     2521 ccggtttcat ggagatcgcc agacacgatc tccccgccct tcctgtgtcc gagcgggtgc
     2581 acaactttcg cgagttcttc ctgcacctgc ccgatgccga gatgagcaag cagggggcgc
     2641 gctgcatgga ttgcggaatc cccttctgtc agaccggctg ccccatcaac aacatcattc
     2701 ccgactggaa cgacttggtt taccgccaga actggcgcga agcgctggac gttctgcact
     2761 cgaccaacaa cttcccggaa ttcaccggcc gcatctgccc ggcgccttgc gaagcagcgt
     2821 gcacgctcaa catcaacgac gatccggtga ccatcaaatc catcgagcac gccatcatcg
     2881 acaaaggctg ggaaatgggt tgggtagtgc cgcagattcc cgcgcgtaaa accggcaagc
     2941 gtgcggctgt ggtgggctcg ggtccggcgg gtctcgcctg ttcgcagcag ctggcgcgcg
     3001 ccggccatga cgtggtgctg ttcgaaaaaa acgaccgcat cggcgggctt ttgcgctacg
     3061 gcattcccga cttcaaaatg gaaaagcacc atatcgatcg ccgcatggag cagatgcgca
     3121 ccgaaggcgt ggaattccgc gtgaactgcc acatcggcgt gaactatccc gcgaaaaagc
     3181 tcctcgacga attcgatgcg gtggtgctga ccggcggatc ggaacacccg cgtgaccttc
     3241 ccgtgccggg aagggattta aaaggcattt actttgcgat ggaattcctg ccgcagcaaa
     3301 acaaggttaa tgccggcgat atcatcccca atcaaatcac cgccaaaggc aaacatgtgg
     3361 tggtgatcgg cggcggcgac accggctccg attgcatcgg cacctccaac cgccagggcg
     3421 cggcctccgt caccaatttc gagctgatgc cgcaaccccc gctgcaagaa aacaaaccgc
     3481 ttacctggcc caactggccg atgaaactgc gcacatcttc ttcccacgaa gaaggctgcg
     3541 accgcgattt tgccattgcc acccagcgct tctctggaaa aggcggccat gtcgccactt
     3601 tgcacacggt aaagctggaa tggaaagccg gcaagatgca ggagatcccg ggcaccgaat
     3661 ttgaagtgaa ggcggacctt gtgctgctgg cgatgggcta tttgtacccg gtgcatgaag
     3721 ggatgctgca ggagctcggc gtggagttcg acccgcgcgg caacgtcaag gccgatacca
     3781 ataaatatca gacttcaatt tccaaagtgt ttgccgcggg cgatatgcgc ctcgggcagt
     3841 cgctggtggt atgggcgata cgcgaaggcc gccaggcagc gcgggctgtt gacgaatttt
     3901 tgatgggttg ctccgacctc ccgcgttaat aaacattcac cgcagaggac acgggagacg
     3961 cagagtaaat ttttcctgaa cacgggtctt gttcaaaatt gatgacttaa tctcgacaaa
     4021 actgggccca aaaccgttaa ttcagaattt ttaatttgct tttcctctga gctctccgcg
     4081 tcctctgcgg tgaataatgt ctaaactcaa aaacgacacc ctgctgcgcg cgctctcacg
     4141 cctgcccact ccttatacgc cggtgtggct aatgcgccag gccgggcgtt atcttcctga
     4201 atacaatgaa acccgaaaac gcgccgggga ttttctagcg ttgtgcaaaa atcccgatct
     4261 tgcctgcgaa gtcacgctgc aacctttggc aagatttaat ttggacgctg cgattctgtt
     4321 ttccgacatt ctcactattc ccgacgccat gggcctcggt ctttatttcg tcgagaacga
     4381 agggccgaag cttgccaaac ccttgcgcga cgagcgggaa atccagaaac tcaagacgcc
     4441 cgaccctgac tcgcatctgc gttatgtaat ggatgcgata agccaaatcc gcaaagcgct
     4501 gggcggcagt gtgcctctca tcggcttctc aggaagtcct tttacgctcg ccagctacat
     4561 gatcgaaggc ggcgggggcc ccgattttcc tcaggtcaaa gagatgcttt atcgtcgtcc
     4621 cgacctgctg catcacgttc tggcggtgaa tactcgcgca ctgaccgctt acttgaacgc
     4681 acaaactgaa tccggcgcgc aggcggtgat gatcttcgac acctggggcg ggatactctc
     4741 aagccacgcc taccacgagt tttcgctgcg ctacaccgaa gaagtgctgc acggcctgat
     4801 ccgcgagcgt gacggcatgc gagtgcccag cattgttttt accaaaggcg gcggcgagtg
     4861 gctggagagc attgccgcca ccggttgcga ttgcgtgagc cttgatagtc ttaccgacat
     4921 cggtcttgcg cgcaggcgcg tgggccataa agtcgcgttg caaggtaatc tcgatcctgc
     4981 cgcgctgttt tcttctccgg aaatcatcgc caaggaagtg gaaaaaattc tggagagctt
     5041 cggcgctcac cccggccatg ttttcaatct cggtcacggc gtgatgccaa ccacgccgcc
     5101 agaaaatgtc gcggcgatgg tggaagcagt ccaccactta agcagaaacc gtcactgaaa
     5161 caagggttaa aaagcgtttc acaaaaaatc gagtgtggtt tcacgcactt tacttatgca
     5221 catctgcatt tgctcttccc aactaaagcc cttgccctat aaggcttttt ctatttgttt
     5281 ttaacattat gatttataag tgttttttat agcttaaatt ttaagcaaaa caacaaagcc
     5341 tttgccggaa gagaaattat cagctgattc aaatttttat ccacaaagtt attcaccgaa
     5401 tttatgcata aaaaaataat tttttgcgta aagaagttag tcgaactagg tagaattcac
     5461 tgtaaaaaat ttccttaact tcgttcttcc cgcatgagca tcgcgcgcgt cgcactcgac
     5521 gttccgctcg acaagctttt tgattacctc gcgccggatt taacccgcga acatatcggc
     5581 ttgcgcgtgc tggtgccgtt tggcaaaaaa caaatagtgg gtgtggtggt ggacattgcg
     5641 gaaaaatcca acatcgcgcc acagcgcttg aagcgcattg tgtcggtttt ccacgacatc
     5701 tccccactcg gtgcgcaaat cctggacgta ttcaaattct gcagtgatta ctatcaccac
     5761 ccactgggtc aggtcatggt caatgcgctg ccagcccggc tgcgccgcag ccgaccgatt
     5821 acgcgcaaaa cctccggtgt cttttgctta accgagacgg ggcggaagct cgacccttct
     5881 gctttgccgg cacaagcggc ggtgaaacgc aaattgtttg aacgtcttaa aagcggggca
     5941 ttaaagaaag aagaactgcg ccaaatttcg cccggcgcgg ccaaggcgct caatgaattc
     6001 atcaccctgg gctgggcgca cgaagtcgcg gcagcctctt cccgccctcc cggccagccc
     6061 gccgacccgc accaactcac ggcggaacaa gccggcgtgg ttgaatcgat tcaactcgcc
     6121 ggattcagct gctcactcct gtttggcgtg accggcagcg gcaagaccga aatctatttc
     6181 aatcggatca cacgggcgct ggcggaaaaa aaacaggtgc tgctgctggt gcctgaaatc
     6241 aatctttcgc cacagctcga agcctggctc aagcagcgct ttccgcaatc ccgggtcgtt
     6301 tctctgcaca gcggcttgaa tgatggtgag cggctggaga actggctcgc tgcccaatcc
     6361 ggcgaggcac aggtaatttt gggtacgcgc cttgcggtgt tcacgcccat tcccaagctc
     6421 gggttcatca tcgtagacga agagcacgac gcttcgttta agcagcagga cggtttgcgc
     6481 tattccgcgc gcgatgtcgc catcttccgc gccaagcagg cggatgtacc ggtcatcctc
     6541 ggctcggcaa cgccggcgct ggaaagctac cagcacgcgc tttccggccg ctaccgcttg
     6601 gcgaaacttg cgcagcgtgc cgtagataat gcgctgctgc ctgaattgcg ctgcatagac
     6661 atacgccgcc tgaagctcaa cgaaggcctg tccgagcccc tgattggcgc catcaggcag
     6721 cggctggaac gcggcgaaca atgcctgatt ttcctcaacc gccgcggcta cgcgccagtg
     6781 ctgatgtgca ccacgtgcaa ctggatttcc ggctgcacgc gctgcacagc cgggctggtt
     6841 ttgcatttgc gggaaaaaaa gctgcgctgc catcactgcg gccatgaaga acgcgtgccc
     6901 gccgcctgcc cgaactgcgg cagcgccgac cttctgccgc tagggcaggg cacgcaacgg
     6961 gtggaaagcg cgttgtccgc tttgtttccc ggcgcgcgcg ttctgcgcat tgaccgcgac
     7021 agtacgcggc gtaagttcgc ctggccggaa atgctcaagc gcatccgcga ccatgaagtg
     7081 gacattctgg tcggcactca aatcctggcc aagggccacg atttccccaa actgtcgctg
     7141 gtcggcatcc tcaacccgga cagctccttg tacagcaccg atttccgcgc gtcagagcgg
     7201 ctgttcgccc agctcatgca ggtcgccggg cgcgccggac gcgcgcaaat ccccggcgaa
     7261 gtgctgattc aaacgcagtt tcccgaccac cccttgtatg aagctttgca gcggcatgat
     7321 tactccgctt tcgctaaaac cctgctggcc gaacgcagac atgcgggatt cccgcccttc
     7381 gtgcaccaag cgctgctgcg cgccgaagct acgcgttcca gcacggtgat gagctatctt
     7441 gctgacgccg ctaagctagc ttcacgcatc agcaatgaag tgacgattta cgatccggtg
     7501 ccggcggcgg tgcaacgcct cgccggccgc gaacgcgggc aattgctggt gcagagccct
     7561 tcgcgcaaaa aactgcagct gtttttaaaa gagtggtatg ccaagttggc cgcagcggac
     7621 aacaagaaag tccgctgggc tttggatgtg gacccgctgg agttttaacg gggtacgcct
     7681 gcttgcccat gggcggtaac tacgtagact atctgcgcag cgagggcatg gcgggctgag
     7741 gcgcgagcga aaggcggagc cgcaagggca gtttcctgcg taagccttct tcattttttc
     7801 ttcgtctccc acaatcccat catgcgtccc tcgaggtccg tgaagagcga gaacgcgccc
     7861 atgccgggca cctcctgctc ttcaacataa atcttgccgc cagccgcgat gattttcttg
     7921 cgataggccg cgagatcgtc gacatcaatg tagagggtta tgttgccggg ccagggttcc
     7981 tcccgctgcg gcttcagtat accgcccatt gataccgcgt tcaccgccag tttcgaccat
     8041 gcggtaattc gtttccggaa tatgctggat cttccagccg aagatatttt catagaaggc
     8101 cgacacctct accggatcct ttgacatcaa ttcccaggga acaacaggtc tgcccatgac
     8161 gatctcctta cgagctaacc attaccttga atccgccgaa gaacatgcgc ttaccgtcga
     8221 aaggccaggg cttcgcttcc atcatcgcgg ccaggcgttt gtccttcata gctttggcat
     8281 tgacgcggtc gcggtgcgcg cgcgatttgt acacgatgta cgaaaagacc accgtttcac
     8341 cgcgctttag cttgacgctc tgcgggaacg acgtccactt gccggacttt acgtcgtcgg
     8401 ccacgcactc gcaaaattcg agggcgccgt gctcccgcca gatcttgccc gacatctgcg
     8461 ccatgcggcg gtaggcctgc aattttttct tcggaacggg tactacaaat ccatctacgt
     8521 atcgcatatg tgtctccttg gaggtgcttt ggttgattac tccgtgacca cgatcgagtc
     8581 gaccttgtcc ggataaaagg ctagccgttc cttgattacg ctcatttcgt cataaggctg
     8641 ctcataagtc cagaccgcgt cctctgctcc gttcttcagg gagtagtacg acgccgtgcc
     8701 cttgaaggga caataggtct gatggttcga gcggacgagc cggtccatct tcacgtcctt
     8761 gcgcgggatg tagtaaacgg cggggtagtc gccctcgtct acccggacgg cgtcctggct
     8821 gtcggcgatg acttcgccca tgaacctcac ctgcacgcgc acgcccgccg gtttggtcgt
     8881 gaggcgatgg ttcgggtatt tcttatatcc gggaccactg ttggtgctca tgatttcacc
     8941 ctccacagtc ccatcatgcg gccctcggga tccgtgaaca gcgagaaagc gcacatgtct
     9001 ggcatcggca tctcgcccat cgccgacatg gatgtgcacc agacgatcgt taccgccgca
     9061 ctggcggcga agagcagcac agagacgcca aaaaaacgct tgctgggagg ctcgatcgga
     9121 agccatgcgt cgcgagatct ccagtcccac agttgcgcaa cgacaaccac gacttcgctc
     9181 agcccttgta ctcgtcgtgg cggcgccacc agacgcccgt ctcgttgcgc cccttggggg
     9241 cgcggtcgag ccactggtac atgccccaaa ggccgtcaag tccgcgcgca taggtggaat
     9301 aggtgtggta gacgacgccg tgctcgagca cgaacgcgct catgcccggc ctctcgcgcg
     9361 tgtacgtggc cacgtcggtt ccggtcatgg ccgcgttatc ggcgacaggc gtccccggca
     9421 tgcggctcgc aatcccctcg tcgtcgcgta actgccacgc cgcctcgcgc cggtagttgt
     9481 attcgaagcc cccctcgcgc tgttgctccg cagtcaacga gacgttgaag tcgaagttga
     9541 agtcgccgcc gtgccaggac gcccagggga aggtccaccc catccgccgc ttgtagtcct
     9601 gcagcttcgc gagcggcgcc cgcgacaccg ccgaaatcgt aacgtcgtgg ttggccaggt
     9661 ggacggcgaa gccgttgaac ccgtccgcga tcgccgagca ggacggacac cccgccgtgt
     9721 agtcgggt
//
LOCUS       DATIOW010000037         2538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1336598, whole genome shotgun sequence.
ACCESSION   DATIOW010000037 DATIOW010000000
VERSION     DATIOW010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336598"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VLL03_01070"
     CDS             complement(<1..392)
                     /locus_tag="VLL03_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HSS45995.1"
                     /translation="MYDPEQPNKWLLKGKLPKRGIYLLPNLFTTAALFAGFYSIVQAM
                     NGRFEFSAIGILVAMVLDGLDGRVARLTHTQSAFGAEYDSLSDMVSFGAAPALVAYVW
                     ALQSMGRFGWIAAFIYCAGAALRLARFNT"
     gene            complement(408..1055)
                     /locus_tag="VLL03_01075"
     CDS             complement(408..1055)
                     /locus_tag="VLL03_01075"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HSS45996.1"
                     /translation="MNYPHPVIAREGWPFLAVAVIASLVVTFIAPWYCSLPLWLLSLF
                     ILQFFRDPPREIPGDGRTVVSPADGRIVAVERAQDPYLRRDALKVSVFMNAFNVHSNR
                     SPVDGMVEKDWYHPGKFLNAALSKASGENERKALWIKTVNGFDVVCVQVAGLIARRIL
                     CYARSGDRLARGQRYGFIRFGSRVDVYLPQDVLVKTSIGDKVYAAESVIAELPMN"
     gene            complement(1170..2186)
                     /gene="ilvC"
                     /locus_tag="VLL03_01080"
     CDS             complement(1170..2186)
                     /gene="ilvC"
                     /locus_tag="VLL03_01080"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HSS45997.1"
                     /translation="MKVYYDKDANLSLIKGRKVAIVGYGSQGHAHANNLQDSGVKVTV
                     GLRKGGASWDKAKKAKLTVKEIGEAVKGADVVTILLPDEHHPEVYRKEIEPNIKKGAA
                     LAFAHGFNIHFDQIVPRADLDVIMIAPKGPGHLVRSTYTQGGGVPSLIAVKQDASGKA
                     RDVALSYACAIGATRAGVIETTFRDECETDLFGEQVVLCGGLTALIQAGFETLVEAGY
                     APEMAYFECLHEVKLIVDLIYEGGIANMRYSISNTAEYGDFTRGPRIINDKTRAEMKK
                     ILREIQSGQFAKEFILENRAGAPSLRAMRRLAAKHPVEEIGTKLRGMMPWIKKNKLVD
                     QSRN"
     gene            complement(2235..>2538)
                     /gene="ilvN"
                     /locus_tag="VLL03_01085"
     CDS             complement(2235..>2538)
                     /gene="ilvN"
                     /locus_tag="VLL03_01085"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HSS45998.1"
                     /translation="LNKLVDVVKVVDLNDGEYIERELMLVKVRAAGKDREEMKRMADI
                     FRGRIIDVTDKAFTIELTGPGSRLDAFLRALEPGAIMETVRTGASGIGRGERILKV"
BASE COUNT          536 a          787 c          662 g          553 t
ORIGIN      
        1 gtgttgaagc gggccagcct gagcgccgcc cccgcgcaat aaatgaaagc ggcaatccag
       61 ccgaaccggc ccatgctctg cagcgcccaa acgtaagcta ccagcgccgg agccgcgccg
      121 aaggacacca tgtcggaaag gctgtcatac tcggcgccga acgcgctctg ggtgtgggta
      181 agccgcgcca cgcgcccgtc cagtccgtcg agtaccatcg ctaccagaat gcctatcgca
      241 gaaaattcga accggccgtt catcgcctgg acaatcgaat agaatccggc aaacagcgcc
      301 gcggtggtga agagattggg caacaggtaa ataccgcgct tggggagctt gcccttcagc
      361 agccatttgt tgggttgttc tggatcgtac atttccataa gctacctcta attcattggc
      421 aattccgcga taaccgattc cgccgcgtat accttatcac cgatgctggt tttgaccaag
      481 acatcctgcg gcaggtagac atccactctc gagccaaaac ggataaagcc atagcgctgg
      541 ccgcgcgcca gcctgtcgcc gctgcgcgcg taacaaagta tgcgcctggc aatcaatcct
      601 gcaacctgca cgcaaacaac gtcaaagccg ttcacggttt tgatccataa cgctttgcgc
      661 tcgttttcac cggaagcctt ggacagcgca gcgttgagga acttccccgg atgataccag
      721 tctttctcga ccatgccatc caccgggctg cggttggaat gcacgttgaa ggcgttcata
      781 aacacgctta ctttcagcgc atcccgccgc aaatacgggt cttgagcgcg ctccaccgca
      841 acgatgcggc cgtctgccgg cgacactacg gtgcggccat cgcccggtat ttcgcgcggg
      901 ggatcgcgga aaaattgcag aatgaacagg ctcagaagcc acaggggcag gctgcaatac
      961 cacggcgcga tgaacgtcac caccagtgaa gcgatgacgg ctaccgccag gaacggccag
     1021 ccttcgcgtg cgatgacggg atgcgggtag ttcatgggtg ggttcagagt tgagggttgg
     1081 gggttcaggg ttgagaatca aacactcgac tctaaactct gaactctcaa ctctcaactc
     1141 acaaaaaacc ctgaaccctc aacttccaat cagtttctcg actgatcgac cagcttgttc
     1201 tttttgatcc aaggcatcat gccgcgcagc ttcgttccaa tctcttctac cggatgtttg
     1261 gcggcaaggc ggcgcatggc tctgagcgaa ggcgcgccag cgcggttttc caggataaat
     1321 tccttggcaa actgtccgct ctggatttct ctgagaattt tcttcatttc ggcgcgggtt
     1381 ttgtcgttga tgatgcgcgg accgcgggta aagtcgccgt attccgcagt gttggaaatc
     1441 gaatagcgca tgttggcgat gccgccttca taaatcagat caacaatcag cttcacttca
     1501 tgcaggcact cgaaataagc catctccggc gcataacctg cttccaccag cgtttcaaac
     1561 cctgcctgta tcagcgccgt caagcctccg catagaacca cctgctcgcc aaacaggtcg
     1621 gtttcgcact cgtcgcggaa agtggtttca atcactccgg cgcgcgtggc accgatggcg
     1681 caagcgtaag agagcgcgac atcccgcgcc ttgcctgagg cgtcctgctt caccgcaatc
     1741 aagctgggaa cgccgccgcc ctgggtgtaa gtcgagcgca ccagatgacc ggggccttta
     1801 ggcgcaatca tgatgacgtc gaggtcggcg cgtggcacga tctggtcgaa gtggatgttg
     1861 aagccgtggg caaacgccag cgccgcgccc ttcttgatgt tcggctcgat ttccttgcga
     1921 tagacctcgg gatggtgctc atcagggagc aaaatcgtca ccacatccgc gccttttacc
     1981 gcctccccga tttccttcac cgtgagcttg gctttcttcg ccttgtccca tgacgcgccg
     2041 ccttttctca ggcccaccgt cactttcacg cccgaatcct gcagattgtt ggcatgcgca
     2101 tggccctgcg agccgtagcc gacgatggcg actttccttc ccttgatcag ggacaggttg
     2161 gcatccttgt cgtaatagac tttcatagtt tcatctccga tgaaaagtgc tcctcactcc
     2221 ttacccctca tctcttacac tttcaatatg cgttcgccgc ggccgatgcc ggaagcaccg
     2281 gtgcgcaccg tctccattat cgcgccgggc tccaatgccc gcaggaaagc gtcgagtctc
     2341 gatcccggcc cggtgagttc aatggtaaaa gccttgtcgg tcacatctat aatgcggccg
     2401 cggaaaatat cagccatgcg cttcatttcc tcgcgatcct tgcccgcggc gcggactttc
     2461 accagcatca gctcgcgctc tatatattcg ccatcgttca aatccaccac tttcaccacg
     2521 tccaccagct tgttcaac
//
LOCUS       DATIOW010000038         3980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1641608, whole genome shotgun sequence.
ACCESSION   DATIOW010000038 DATIOW010000000
VERSION     DATIOW010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641608"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..105
                     /locus_tag="VLL03_01090"
     tRNA            30..105
                     /locus_tag="VLL03_01090"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:63..65,aa:Val,seq:tac)
     gene            129..205
                     /locus_tag="VLL03_01095"
     tRNA            129..205
                     /locus_tag="VLL03_01095"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:163..165,aa:Asp,seq:gtc)
     gene            296..2179
                     /locus_tag="VLL03_01100"
     CDS             296..2179
                     /locus_tag="VLL03_01100"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HSS45999.1"
                     /translation="MFDFFQRHKRVAQIVLALIFLPFALFGVERYFSSIGGGDYVAKV
                     GSYEISSQEFTQALRDEQEKMRSQMGNQFSPVILEDPEVRAAILENLIRKHLLLSEAG
                     RSGLTVTDTQLQKIITQFQPFQENGKFSRTRYEALLRNQNLTPLGFEARLRQDIVLQQ
                     FNDAFETGFLPQTVAERLIRLSEQQREVSQYLLSPDQFLLQIKLPDAAARNYYEAHRN
                     EFQVPERVRADFVVLSVDGLMSQVQVSADETRKYYNEHTSQFETRQASHILITVPANA
                     SPEAKAQARAKAEQLYQEARKNPEKFAELAKANSQDPGSAGNGGDLGLISRGAMVKPF
                     EEAVFQMKAGEIAGPVETQFGYHVIKLNAVKPVTFDEAKAQVERDLKKQQAEKKFNEL
                     AENFSNMVYEQPDSLKPVADAFKLPVQHAPWLSRDGDGAPGPLAGEKMLQALFSADAL
                     KNKRNTEAIEVAPSILVAAHVVEYKPASVKPLDEVKGDINRRLLLEKGSELAKKDGME
                     KLAKLKEGKETGISWSAPRMVARQHASPDLDANALREVFKADVARLPAYAGVELPQGG
                     FALLKISRVEEVGRIDEAKRKNFAGQLLQFIAEEELSAYLASLRQKTEVKINQQALEK
                     KER"
     gene            complement(2189..2983)
                     /gene="fabI"
                     /locus_tag="VLL03_01105"
     CDS             complement(2189..2983)
                     /gene="fabI"
                     /locus_tag="VLL03_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HSS46000.1"
                     /translation="MGFLAGKHILITGLLSNRSIAYGIAKAMQREGAQLAFTYQGERV
                     QARVAEMATQEFKSELVFPCDVADDQQIQQLFGGLGKHWGGLDGIVHSIAFAPREALA
                     GDFLEGLSREAFRIAHDVSSYSFAALAKAGLPLMQGRKAALLTLTYLGALRVLPNYNV
                     MGLAKASLEANVRYLAHSLGPKGIRVNAISAGPIKTLAAAGIGGFGKILHFVAENAPL
                     RRNVTIEDVGNAAAFLMSDLANGITGEITYVDAGFNTAVGGIPELS"
     gene            3100..>3980
                     /locus_tag="VLL03_01110"
     CDS             3100..>3980
                     /locus_tag="VLL03_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HSS46001.1"
                     /translation="MNGHFIRCISLVLPLFAAGCGGSTWNNPYPAGDGGKNILYSAFT
                     ERPKHLDPVQSYSENEAVFNAQIYEPPLQYHYLKRPYTLVPLSAMEMPHPRYFDKDGR
                     SLPDNADPKRIAYSIYDIRIKPGIHYQPHPAFARDASGKLIYHNLTPEALQNIYALSD
                     FKERGTRELTADDYVYEIKRLAHPRLHLPIFGLMSDYIAGLKEYAETLKKANQELIAK
                     EGKDIWLDLTRYPLEGAQVLDRYSYRIKIKGKYPQFVYWLAMPFFAPVPVEVDRFYSQ
                     SGMAEKNLTLDWYPVGR"
BASE COUNT          896 a         1131 c         1162 g          791 t
ORIGIN      
        1 ggcaatcgag aatacctcgg ccgggtttag ggtgcttagc tcagttggta gagcgtcgcc
       61 cttacatggc gaatgtcggg agttcgaccc tctcagcacc caccagcgga attcagggtt
      121 tgattagagg agtggtagtt cagttggtta gaataccggc ctgtcacgcc gggggtcgcg
      181 ggttcgagtc ccgtccactc cgccaccgat tggcagggaa cctcatcaga gattccttaa
      241 gaggcgaaca ccggttcgcc ttttttatat cccgagcagg agcccgtctc ataacatgtt
      301 tgattttttt cagcgccaca agagagtcgc ccagatcgtc ctggctttga tcttcctgcc
      361 gtttgcgctg ttcggcgtgg aacgctattt cagcagcatc ggcggcggag attacgtggc
      421 taaggtcggc agctatgaga tcagctcgca ggaatttacc caggcgctgc gcgatgagca
      481 ggaaaaaatg cgcagtcaaa tggggaatca gtttagtccg gtgattctgg aagaccccga
      541 agtgcgcgcg gcgattctgg agaatctgat acggaagcat ctgcttctct ccgaagccgg
      601 gcgttccggg cttaccgtca ccgatacaca actgcagaaa atcatcactc aattccagcc
      661 attccaggaa aacggcaaat tttccagaac gcgctacgaa gccttgctgc gcaaccagaa
      721 tcttacgccg ctcggatttg aagcccggct gcgtcaggac atcgtgttgc agcagttcaa
      781 cgatgccttc gagaccggct tcctgccgca aacggtggcc gagcggctga tccgcctgag
      841 cgagcagcag cgtgaagtca gccaatacct gctctcaccc gaccagtttc tgttgcaaat
      901 caagctcccc gacgctgccg ccaggaatta ttacgaagcg catcgcaatg aattccaggt
      961 accggaacgg gtgcgcgccg attttgtggt gctctcggtg gatggcctca tgtcgcaggt
     1021 gcaagtgagc gcggatgaga cccggaaata ctacaacgag cacacttcgc aattcgagac
     1081 acggcaggcg agccacatcc tcattaccgt tccggctaat gctagccccg aagccaaggc
     1141 tcaagcccgg gccaaggccg agcagttgta tcaggaagcg cgcaagaatc cggaaaaatt
     1201 cgccgagctc gccaaggcca attcgcagga cccgggctcg gcgggaaatg gcggcgatct
     1261 gggattgatc tcacgcggcg ccatggtgaa gccgtttgaa gaggcggtgt tccagatgaa
     1321 ggcaggcgaa attgccggcc cggtggagac tcagttcggc tatcacgtca tcaagctcaa
     1381 cgccgtcaag cccgtgacgt ttgacgaggc aaaagcccag gtcgagcgcg acttgaaaaa
     1441 acagcaggca gagaaaaaat tcaacgagct cgccgagaat ttcagcaaca tggtgtatga
     1501 gcagcctgac agcctcaaac cggtggccga tgcgttcaag ctgcccgtgc agcatgcgcc
     1561 gtggctgtcg cgtgacggcg acggtgcgcc gggacctttg gccggagaga agatgctgca
     1621 agcgctgttt tccgcggatg cgctcaagaa caagcgcaat accgaggcga ttgaggtcgc
     1681 tccgagtatc ttggtggccg cgcatgtcgt cgaatataag ccggcgagcg tgaagccctt
     1741 ggacgaggtc aaaggcgata tcaaccgccg cctgctgctg gaaaaaggat ctgaactcgc
     1801 gaaaaaggac ggcatggaga agctggccaa gctcaaggaa ggcaaggaaa cgggcatttc
     1861 ctggagcgca ccgcgcatgg tggcgcggca gcatgcttcg ccggacctcg atgccaacgc
     1921 gctgcgcgaa gtgttcaagg ctgatgtggc caggcttccc gcttacgcgg gcgtggagct
     1981 cccgcaaggc ggtttcgcgc tgctcaaaat cagccgggtg gaggaggtgg gacggataga
     2041 tgaagccaag cgcaagaatt ttgccggcca gctgctgcag ttcatcgcgg aggaggagtt
     2101 atccgcttat ttggccagcc tcaggcaaaa aacggaagtg aaaatcaacc agcaggcgct
     2161 ggagaagaaa gagcgctgag agcaggattc aggaaagttc cgggatgccg ccgacggcgg
     2221 tgttgaaccc cgcgtccacg taggtgatct ctccggtaat gccgtttgcc aggtcgctca
     2281 tcaaaaacgc ggcggcattg cccacatcct caatcgtcac attgcgcctg agcggtgcgt
     2341 tctccgcgac gaaatgcagt atttttccga agccgcctat acccgccgcg gccagggtct
     2401 tgatcgggcc ggcggaaatg gcgttgacgc ggatgccctt cgggcccagg ctgtgcgcca
     2461 gatagcgcac attggcttcc aggcttgctt tcgccagacc catgacattg taattgggca
     2521 gaacgcgcag cgctcccaga taggtgaggg tgagcagcgc cgcttttctt ccttgcatca
     2581 gcggcaagcc ggctttggcc agcgcggcaa agctgtagga gcttacatcg tgggcgatgc
     2641 ggaatgcctc gcgggagagc ccctccagaa aatcgccggc gagcgcctcg cgcggagcga
     2701 acgcaataga atgcacaata ccgtccagcc cgccccagtg cttgccgagg ccgccgaaca
     2761 attgttggat ttgctgatcg tcggccacat cgcagggaaa gaccagctcg cttttaaatt
     2821 cttgtgtcgc catttccgcc acccgggcct gcacccgctc gccctggtaa gtaaaagcga
     2881 gctgggcgcc ttcgcgctgc atcgccttgg caatgccgta ggcaatggaa cgattgctga
     2941 gcaatccggt aatcaggatg tgctttccgg cgagaaatcc catgattgtt cctgattggt
     3001 tccgcggaaa caaaattata gcctagtttg cctcagcatt acttgcggtg ggcttgccaa
     3061 gggtgacgct gggaggcggt ttgtatacac tgtcgttcga tgaatggaca ttttatccgc
     3121 tgcatttccc tggttttgcc cctgtttgcc gcaggctgcg gcggctccac ctggaataat
     3181 ccttatcccg ccggagacgg cggcaaaaac attctctact cggccttcac cgagcggccc
     3241 aagcatctcg acccggtgca gtcgtacagc gagaacgaag cggtattcaa tgcgcaaatc
     3301 tacgaaccgc cgctgcaata tcattacctc aaacgcccct acaccctggt gccgttaagc
     3361 gcgatggaaa tgccgcatcc gcggtatttc gacaaggatg ggcgctctct ccccgacaat
     3421 gccgacccga aacgcatcgc ttacagcatt tacgatattc gcatcaagcc gggcatccat
     3481 tatcagccgc atccggcatt cgccagggat gcaagcggca agctgattta tcacaacctg
     3541 accccggagg cgctgcagaa catttacgcc ctctccgatt tcaaggaacg gggcacgcgc
     3601 gaactcaccg ccgatgacta tgtttacgaa atcaagcgtc tcgcccatcc gcgactgcat
     3661 ttgccgattt tcggcctgat gagcgattac atcgcgggcc ttaaggaata cgcggagacg
     3721 ctgaaaaaag ccaaccagga attgattgcc aaagagggca aagatatctg gctcgatttg
     3781 acgcgttatc ccctcgaagg cgcgcaagtt ctcgaccgct acagctaccg catcaagatc
     3841 aagggcaaat acccgcagtt tgtttactgg ctggcgatgc cattcttcgc gccggtgccg
     3901 gtggaagttg accgctttta ttcgcagtcc ggcatggcgg aaaaaaacct cacgctggac
     3961 tggtacccgg tcggcagaga
//
LOCUS       DATIOW010000039        11028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1540005, whole genome shotgun sequence.
ACCESSION   DATIOW010000039 DATIOW010000000
VERSION     DATIOW010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11028
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540005"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1175
                     /gene="dusB"
                     /locus_tag="VLL03_01115"
     CDS             162..1175
                     /gene="dusB"
                     /locus_tag="VLL03_01115"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HSS46002.1"
                     /translation="MRIGFHQLKNNLIVAPMAGVTDRPFRQLCKRMGAGMAVSEMVSS
                     NSLLWGSEKTRRRANHEGEVEPVVVQIAGADPRMLAEAAKYNVDQGAQIIDINMGCPA
                     KKVCNVMAGSALLQNEPLVRRILDAVVKAVNVPVTLKIRTGWDRQNKNALRVAGIAEN
                     AGIQALTIHGRTRACGFSGTAEYDTIATVKAHLKIPVIANGDIATPEKVKEVLDYTKA
                     DAVMIGRAAQGRPWIFREIGHYLKTGTHLASPEVAEIRRVLVAHLNELYEFYGEYTGV
                     RIARKHISWYTRGLACSAQFRHAMNQLETSAAQLVAVNCFFDELQSRGRCLVYEESEE
                     LAA"
     gene            1175..1411
                     /locus_tag="VLL03_01120"
     CDS             1175..1411
                     /locus_tag="VLL03_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSS46003.1"
                     /translation="MDENELTRCVRKAIDGYFKDLDGEKPCAIYDMVLRCVEKPLLES
                     VLHRAKGNQTHAAQMLGLNRNTLRKKMQEHRIKG"
     gene            1529..3085
                     /gene="purH"
                     /locus_tag="VLL03_01125"
     CDS             1529..3085
                     /gene="purH"
                     /locus_tag="VLL03_01125"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HSS46004.1"
                     /translation="MIKQALISVSDKTGIVEFAKGLSRFGVILLSTGGTAKLLSQSGV
                     KVTEVGDYTGFPEMLEGRVKTLHPKVHAGILARRDVPRHMQAMSKANIPTIDMVVVNL
                     YPFRETIAKPGCTLEEAIENIDIGGPTMVRAAAKNHQHVAAVTDPADYQELLAEMQSA
                     NGAIGLETRFRLAQKAFSHTAAYDGAISNYLTATNLEGEHIAFPNQINFNFNKVQDLR
                     YGENPHQNAAFYRDLASVPGSLSEYQQIQGKELSYNNIADSDAAWECVKTFDKPACVI
                     IKHANPCGVAVGKDALQAYKLAFATDPTSAFGGIIAFNRPVDGAAAAAVIQQFVEVLI
                     APGFSEDAKRILGQKANIRVLIVPIAEGANNLDIKRVGGGILVQMPDNLNVAASQLKV
                     VTKKQPTSLQLDDLLFAWRVAKFVKSNAIVFAGNGQTLGVGAGQMSRVDSARIASIKA
                     QNAGLNLQGSVVASDAFFPFRDGVDVVVQAGARAVIQPGGSMRDDEVIAAADEHGIAM
                     VFTGVRHFRH"
     gene            3193..4470
                     /gene="purD"
                     /locus_tag="VLL03_01130"
     CDS             3193..4470
                     /gene="purD"
                     /locus_tag="VLL03_01130"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HSS46005.1"
                     /translation="MKLLVIGGGGREHALAWKLAQSPKIQKVFVAPGNGGTALESDLQ
                     NVAIEGIPDLVAFVKKEKIHFTVVGPEAPLSKGVVDAFKEANLRIFGPTRKAAQLESS
                     KDFAKSFMRRHGLPTAAYSTFADSAKAHAYLDSRGAPIVIKADGLAAGKGVVVAMNLA
                     DAHAAVDMMLVSTKMGVAGARVVIEDYLKGEEASFIVMCDGEHILPLATSQDHKRLLD
                     GDQGPNTGGMGAYSPAPVVTPALHNRVMREIIQPVIKGMAEDGISYTGFLYAGLMIDG
                     RGNPRVLEFNCRMGDPETQPILLRLKSDLFKMVEHAINGTLDKIEADWDRRAALGVVL
                     AAANYPDTPKTGDMISGLPKPGEDFKIFHAGTQLNGKNVVTSGGRVLCVTALGDNVKM
                     AQRRAYEIAEGIHFDGMQMRGDIGHRATGLKKH"
     gene            4663..5586
                     /gene="hemF"
                     /locus_tag="VLL03_01135"
     CDS             4663..5586
                     /gene="hemF"
                     /locus_tag="VLL03_01135"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HSS46006.1"
                     /translation="MNPDAVKNYLTDLQSRIVVRLAQFDGKIFGADSWERPEGGGGLS
                     CVIEEGQVFERGVVNFSHVFGEKLPRSATASRPELAGRTFEATGVSVVMHPRNPYAPT
                     VHLNIRFIRAFVKKGAAGQAAIAPDVWWFGGGMDLTPYYGFQEDCVHFHQTCKDALAP
                     FGSEYYPRFKKWCDQYFFIKHRNEPRGIGGIFFDDFNEPGFEKSFQLMQSVGENFLKA
                     YPPILERRINLPYGERERDFQAYRRGRYVEFNLVYDRGTLFGLQSGGRTESILMSLPP
                     SVKWRYDWKPEPNSAEEKLYKEFLIAQDWVN"
     gene            5615..6034
                     /locus_tag="VLL03_01140"
     CDS             5615..6034
                     /locus_tag="VLL03_01140"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HSS46007.1"
                     /translation="MSYKTDQIQKIGSGLVDAFHYLALFVIGATIVWSAVFEYLRIMQ
                     SGHAALEDILLLFIYLELGAIVGIYFKTHRLPVQFLIYVAITALSRHLVIDIQKVSDN
                     FHLYLLLSITGAIVILSFAVLILTFAASKYGRPEDNA"
     gene            complement(6042..6569)
                     /locus_tag="VLL03_01145"
     CDS             complement(6042..6569)
                     /locus_tag="VLL03_01145"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HSS46008.1"
                     /translation="MDIKSLIRTIPDFPKPGILFRDITTLLKHNDGFRSTIDQLFGKY
                     RGMRIDKVAGIESRGFIIGAALAYHLKAGFVPIRKKGKLPGENFGHDYELEYGTDRLE
                     IHTDAIQKGEKVLLVDDLIATGGTAEAAAKLIGKTGGQIIGCAFVIDLPDLGGRKRLE
                     KLGHSVVTLCEFEGH"
     gene            complement(6573..7277)
                     /locus_tag="VLL03_01150"
     CDS             complement(6573..7277)
                     /locus_tag="VLL03_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSS46009.1"
                     /translation="MNLLEVRGLKVSYGGINAVKGIDINIGEGELVALIGCNGAGKST
                     TLKAICGLLLHMEGEVRYQGRLITRSPSYQLVRRGMALVPEGRGIFGRLTVQENLEMG
                     AYCRPSDSKSGLERVYGLFARLKERRSQTAGTLSGGEQQMLAIGRALMSRPKLLLLDE
                     PSMGLAPLMVSKIFETILSIAKEGVTLLLVEQNARLALEASHRGYVMDGGLITLSGAA
                     RELLNNPQVREAYLGE"
     gene            complement(7274..8038)
                     /locus_tag="VLL03_01155"
     CDS             complement(7274..8038)
                     /locus_tag="VLL03_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSS46010.1"
                     /translation="MNLLEARGVSKRFGGLLALDEVSLFIERREIYGLIGPNGAGKTT
                     FFNVLTGLYAPDNGEFFFNGAKLTATKPHAVAEQGIARTFQNIRLFANMTALENVMVG
                     RHVRTRAGVIGAVLRDARTRAEEEAIRSRASTLLEYAGIAQRANDLAKHLSYGDQRRL
                     EIARALATEPKLLALDEPAAGMNATETIGLKRLLETIRGDGTTILLIEHDVKLVMGLC
                     DRVAVLDYGKKIAEGAPQQVQRDPAVIEAYLGGALA"
     gene            complement(8035..9135)
                     /locus_tag="VLL03_01160"
     CDS             complement(8035..9135)
                     /locus_tag="VLL03_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006383978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSS46011.1"
                     /translation="MNRWEKLKRNPRAAWAAFILLAVLLAVLPFLADAALGRTWVRIL
                     DFALLYIMLALGLNIVVGYAGLLDLGYVAFFAVGAYVYALLASPHFGLHLPFWVLLPL
                     GAGVTCIFGVLLGAPTLRLRGDYLAIVTLGFGEIIRIFLNNLNAPVNITNGPQGVNRI
                     DPVSLGNFSLSQTHQLAGINFSSVHNYYYLFLALTVLVIFFSMRLQDSRIGRAWIAIR
                     EDEVAAKAMGINTRNVKLLAFAMGASFGGVSGGLFAGFQGFISPESFTLIESITILCM
                     VVLGGMGHIPGVILGVLLLAILPEALRYIGPLEQALFGRIIVDPADLRMLVFGLALIL
                     VMLFRPAGLWPSTTRKREFAAENEMAKSEARA"
     gene            complement(9136..10059)
                     /locus_tag="VLL03_01165"
     CDS             complement(9136..10059)
                     /locus_tag="VLL03_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSS46012.1"
                     /translation="MDIFLQQIINGIVLGSVYALVALGYTMVYGILGLIIFAHGEVVM
                     IGAMVALAVIHALAGSALPGPVIVLLGLLVAMPTCMVLGYGIERIAYRPLRNAPRLAP
                     LITAIGMSIVLQNSAMIIWGRQYITFPPLLPSKPHFIAGAAISDLQIFIVLLAALIMA
                     GLLFLVHRTRLGRAMRATAQNAEVAGLMGVNINTIISITFVVGSALAAVAGVMVSGYY
                     GLAHYYMGFSLGLKAFTAAVLGGIGNFGGAMLGGLLLGIIESLGAGYIGLLTGGFLGS
                     NYQDVFAFFVLILVLVFRPSGLMGERIAERA"
     gene            complement(10120..>11028)
                     /locus_tag="VLL03_01170"
     CDS             complement(10120..>11028)
                     /locus_tag="VLL03_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HSS46013.1"
                     /translation="NAVIGHLNSGATIPASKIYHDADVVQISPSATAIPYTQQGFKNA
                     FRVMTNDEQQGKVLGEFAVSQMAAKKIAIIDDRTAYGQGLADQFEKAAKAGGAEVLAR
                     EYTTDRATDFLAILTSIKGKQPDLVFYGGMDAQAGPMVKQMRSLGLNAKFLGGDGTQS
                     NEFIKIAGANAEGVTASSPGLPLGKMPGGSDFLKKFESKYGKIQIYAPYAYDATMTLI
                     EAMKKADSLEPAKYLPVLAQIQRNGVTGPIAFDAKGDVKGGAVTLYQVKGGNWVPLQT
                     LGQEAPPAQQDAAAPASAAGSTRQGY"
BASE COUNT         2483 a         3106 c         3127 g         2312 t
ORIGIN      
        1 gattaaattt ttttgcctta cacagtttct gattttgtca aagtcgattt tttcctgagt
       61 tgctcgcgtt ttaatacgag ttttgcaaga aatttataat ccgcttttct tggaaccgta
      121 attgcgctta caattacaac cctgcctcaa aaaataacat catgcgaatc ggtttccacc
      181 agctgaagaa caatctgatt gtcgcgccca tggccggcgt taccgaccgc ccgttccggc
      241 agttgtgcaa gcgcatgggc gcgggcatgg cggtgtccga aatggtttcc agcaattctc
      301 tgctgtgggg ttcggagaaa accaggcgcc gtgccaacca tgaaggcgaa gtggagccgg
      361 ttgtggtaca aatagccggc gccgaccccc gtatgcttgc cgaagccgcg aaatacaacg
      421 ttgatcaagg ggcgcagatc atcgacatca acatgggttg tcccgccaaa aaggtctgca
      481 acgtcatggc gggctcggcg cttttgcaga acgagccact ggtgcggaga attcttgatg
      541 cggtggtaaa agcagtaaac gtgccagtca cgcttaaaat ccgcaccggc tgggaccggc
      601 agaacaaaaa tgcattgcgc gtggcgggca ttgccgagaa cgccggcatt caggcattga
      661 cgattcatgg cagaacgcgc gcctgcgggt tttccggcac tgccgagtac gacaccatcg
      721 ccacggtgaa ggctcacttg aaaatccccg tgatcgccaa cggcgacatc gcgaccccgg
      781 aaaaagtcaa ggaagtgctt gattacacca aggccgacgc ggtgatgatc ggccgcgcgg
      841 cgcaggggcg gccgtggatt ttccgcgaaa tcggtcatta ccttaaaact ggcacgcatt
      901 tggcttcgcc cgaagttgcg gaaatccgcc gcgtgctggt tgcgcatctg aacgagcttt
      961 atgagtttta cggcgaatac accggcgtgc gcatcgcccg caagcatatt tcctggtaca
     1021 ccaggggctt ggcctgctcg gcgcagtttc gccacgccat gaaccaattg gaaacgagcg
     1081 cagcgcagct cgtggcggta aattgtttct tcgacgaatt gcaaagccgc ggccggtgtc
     1141 tggtctatga agaatcagag gaattggcgg cataatggat gagaatgagc ttacgcgctg
     1201 tgtgcgcaag gccatcgatg gctatttcaa ggatttggac ggcgaaaaac cctgtgccat
     1261 ttacgacatg gtgctccgct gtgtggaaaa gccgctgctc gaatcggtgc tgcaccgcgc
     1321 caaggggaat caaacgcatg cggcacagat gctgggcctg aaccgtaaca ccctgcgcaa
     1381 aaaaatgcag gagcaccgga taaaaggtta gggaacccag gaagtccctt agggtttaga
     1441 attcagggga gaagaatgca ccgggccaag cctcgcttgc cctgaattgt caatcccggc
     1501 tcatcttttc atcccgcaca tcatgagcat aatcaaacag gcgctgatca gtgtttccga
     1561 caaaaccggc attgtcgaat ttgccaaggg cttgagccga ttcggggtca tccttctttc
     1621 caccggtggc accgccaagc tgctttccca atccggcgtg aaagtcaccg aagtgggcga
     1681 ttacacgggc tttcccgaaa tgctcgaagg ccgggttaag actctgcatc caaaagtgca
     1741 cgcgggcata ctcgcgcgac gcgatgtgcc caggcacatg caggcgatga gcaaagccaa
     1801 cattccgacc atagacatgg tggtggtgaa tctctatccg ttccgcgaga ccatcgctaa
     1861 gccgggttgc acgctggaag aagcgataga aaatatcgac atcggcggcc ccactatggt
     1921 gcgcgcggca gccaagaatc accagcatgt agcggcggtg accgatcccg ctgattacca
     1981 ggaattgctg gcggaaatgc aatcggccaa cggcgccatc ggcctggaga cgcgcttccg
     2041 tctcgcgcaa aaggcgttct cgcatacggc ggcttacgat ggcgccatca gcaattatct
     2101 gaccgcaacc aatctcgagg gcgagcacat cgccttccca aatcagatta acttcaactt
     2161 caacaaggtg caggatttgc gctatggcga gaatccgcac caaaatgcgg cgttttaccg
     2221 tgacctagct tccgttccgg gcagcttgag cgaataccag cagattcagg gcaaggagct
     2281 ttcctacaac aacatcgccg attccgacgc cgcctgggag tgcgtgaaaa cttttgacaa
     2341 gccggcgtgc gtgattatca agcacgccaa tccttgcggt gtcgccgtcg gcaaagacgc
     2401 actgcaggca tataaactgg cctttgccac cgatcccact tcagctttcg gcggcatcat
     2461 cgctttcaac cgcccggtgg acggcgccgc cgccgcagcg gtgattcagc aattcgtcga
     2521 agtgctgatt gcgccgggat tttcagaaga cgcgaagcgc atcctagggc aaaaagccaa
     2581 tatccgcgtg cttatcgtgc cgattgccga aggcgccaac aatctcgata tcaagcgcgt
     2641 gggcgggggg atactggtgc aaatgccgga caatctcaac gtcgcggctt cgcaactcaa
     2701 ggtggtgacc aaaaagcagc ccacttcgct gcagctcgac gatttgctgt ttgcctggcg
     2761 cgtcgccaag ttcgtcaaat ccaacgccat cgttttcgcc ggcaacggcc agaccctggg
     2821 ggtgggcgcg gggcagatga gccgggtgga cagcgcgcgc atcgcttcca tcaaggcgca
     2881 gaacgccggt ttgaatctgc aaggctcggt agtggcctcc gacgcgtttt ttccgttccg
     2941 tgacggtgtg gacgtggtgg tgcaggccgg cgcaagagcg gtgattcaac ccggcggcag
     3001 catgcgcgat gacgaagtga ttgccgcggc ggatgagcat ggaattgcga tggtgtttac
     3061 cggcgtgcgg cattttagac actaacttat taaccacgga gacgcaaagg cgcagagaac
     3121 ttcaaaagcc tttataaaga aggtaataaa atgaacagct tttctccgcg cctctgcggt
     3181 tactcttcaa tcatgaagct cctcgtcata ggcggcggcg gcagggagca tgcactggcc
     3241 tggaagctcg cgcaatcgcc aaaaattcaa aaagtcttcg tcgctcccgg caatggcggc
     3301 accgcgcttg agtctgattt gcaaaacgtg gcgatagaag gaattcctga tctcgtcgcg
     3361 ttcgtcaaaa aagaaaaaat ccatttcacc gtggtcggtc ccgaagcgcc gctttccaaa
     3421 ggcgtggtgg atgcgttcaa agaagcaaat ctcagaatct tcggccccac gcgaaaagcc
     3481 gcgcagctcg aaagctccaa ggatttcgcc aaaagcttca tgcggcgtca cggccttccc
     3541 actgccgcct attccacatt cgccgattcc gccaaagcgc acgcttacct tgacagccgc
     3601 ggcgcgccca tcgtgatcaa agccgatggc cttgccgccg gcaagggcgt ggtggtggcg
     3661 atgaatctgg cagacgcgca cgccgcggtg gacatgatgt tggtttccac aaaaatgggc
     3721 gtcgccggcg cacgggtagt gatcgaggat tatctgaaag gcgaagaggc gagcttcatt
     3781 gtcatgtgcg acggcgagca tattcttccg cttgccacca gccaggacca caagcggctg
     3841 cttgatggcg accaaggacc gaataccggc ggcatgggtg cgtattcgcc cgcgccggtg
     3901 gtgacacccg cgttgcacaa tcgggtgatg cgcgaaatca tccagccggt gattaaaggc
     3961 atggcggagg atggcatttc ttataccggt tttctctatg cgggattgat gatagacggc
     4021 cggggcaatc ccagggtgct ggaattcaac tgccgcatgg gcgaccccga aacccagcca
     4081 attctcctgc ggctcaaaag cgatttattc aagatggtcg agcacgcaat aaacggcaca
     4141 ttggataaaa tcgaggccga ctgggaccgc cgggcagcgc tgggtgtggt actcgccgcg
     4201 gcgaattatc cggatacgcc gaagacaggc gacatgatta gcggcctgcc caaaccgggt
     4261 gaggatttca agatattcca cgccggcact caactcaacg gaaagaatgt cgtcaccagc
     4321 ggcggccggg tgctgtgcgt tacggctttg ggcgacaacg tcaaaatggc gcagcgccgc
     4381 gcttacgaaa tcgccgaagg catccatttc gatggcatgc aaatgcgcgg cgacatcggc
     4441 caccgcgcca ccggattaaa aaaacactga ttcaccgcag agacgcagag gcaggagaag
     4501 aacttataaa aaataaatct gtatttactt catgttttaa gcagtcatcc tgagctatgc
     4561 gaaggatctg ctttctaagt taaactgacg ctttcacaac aaagttcttt taaggaaact
     4621 atttgccttg tgcctctgcg tctccgcggt taaaaatttc tcatgaatcc cgatgcagtc
     4681 aaaaattacc ttaccgattt gcagtcacgc atcgtcgtgc gcctggcgca gtttgacggc
     4741 aagatatttg gcgccgattc ctgggagcgt cccgaaggcg gcggcggact aagctgcgtc
     4801 atcgaggaag gccaggtatt cgagcgtggc gtggtgaatt tctcgcatgt atttggcgaa
     4861 aagcttccgc gttctgccac cgcttcgcgc cccgagctgg cgggccgcac ttttgaagca
     4921 acgggcgtgt ccgtagtcat gcatccgcgc aatccttatg ctcccacagt acatctcaac
     4981 attcgattta tccgggcatt cgttaaaaag ggtgcagcgg gacaggctgc gattgcgccc
     5041 gatgtatggt ggtttggggg aggtatggac ctaacgcctt actacggatt ccaggaagac
     5101 tgcgtgcatt ttcaccaaac ctgcaaagat gcgcttgcgc ctttcgggtc agaatactac
     5161 ccgcgcttca agaaatggtg cgaccagtat tttttcatca agcaccgcaa cgagccgcgc
     5221 ggcatcggcg ggattttttt cgatgacttc aacgagccgg gtttcgagaa aagtttccag
     5281 ctcatgcaaa gcgtgggcga aaattttctc aaagcctacc cgccgattct ggaacgccgc
     5341 attaacctgc cctatggcga gcgcgagcgc gacttccagg cctaccgacg cgggcgctac
     5401 gtggagttta atctggtgta cgaccgcgga acgttgttcg gcctgcagtc cggcggacgc
     5461 accgaatcca tcctgatgtc attgccgccg agtgtgaaat ggcgctacga ttggaaaccc
     5521 gagccaaaca gcgcggaaga aaagttatac aaagagtttt tgattgcgca ggactgggtt
     5581 aattaagctg tttatttcta ttggaattac aacaatgagt tataaaactg atcaaatcca
     5641 aaaaatcggc agcggactgg tagacgcatt tcattacctt gcgctgtttg ttatcggtgc
     5701 cactattgtt tggtcggccg tgtttgaata cctgcgcatt atgcaaagtg gacatgcggc
     5761 tttggaagat attctgttgc tgtttattta tcttgagctc ggcgcgatag taggaatcta
     5821 tttcaaaacc catcgtttgc ctgtgcaatt cctgatttat gtcgcaatta cggctctttc
     5881 acggcatttg gtcattgaca tccagaaggt atcggataat tttcatctct atctgctgct
     5941 gagcataacc ggagccatag tgatactcag cttcgcagta ttaatattga cattcgctgc
     6001 cagcaagtac gggcggcctg aggacaacgc ttgacctatt tctaatgccc ttcaaactcg
     6061 cacaatgtaa ccacgctgtg accgagtttt tccaagcgct tgcggccgcc gaggtccggt
     6121 aaatcaatca caaacgcaca gccgataatc tgtccgccgg ttttgccaat cagcttggcg
     6181 gccgcttccg ccgtgccgcc ggttgcaatc aaatcatcca ccaataaaac tttttcccct
     6241 ttttgaatcg cgtcggtgtg gatttcaagc cggtcggtgc cgtattccag ttcgtagtcg
     6301 tgcccgaaat tttcacccgg aagtttccct ttcttgcgga tcggtacgaa gccggctttg
     6361 aggtggtagg cgagtgccgc gccgatgatg aagccgcgcg attcgattcc cgcgactttg
     6421 tctattctca tgccacggta tttaccgaac agttggtcta tggtgctgcg aaaaccgtcg
     6481 ttgtgtttga gcagcgtggt aatgtcgcga aacaaaattc ccggctttgg aaaatcagga
     6541 atggtacgaa tcagcgattt gatgtccatc gtttattctc caagataagc ttcgcgtacc
     6601 tgcggattgt tcagtagttc gcgcgccgcg ccggaaagcg taatcagccc gccatccatg
     6661 acataaccgc ggtggcttgc ctccagggcg agcctggcgt tttgctcgac cagcagcagc
     6721 gtcacgcctt ccttggcgat gcttaagatg gtctcgaaaa tcttactcac catcagcggc
     6781 gccaaaccca tgctcggctc gtcgagcaac agcaatttcg ggcggctcat cagcgcgcgg
     6841 ccaatcgcca gcatttgctg ttcgccgccg gaaagcgtgc ccgcggtttg gctgcgccgc
     6901 tctttgaggc gagcgaacag tccgtacacg cgctcgaggc cgctttttga atcggagggg
     6961 cggcaatatg cgcccatttc caggttttct tgcaccgtga gcctgccgaa aatgccgcgg
     7021 ccttccggca ccagcgccat gcctcggcgc accagttgat aagagggcga acgagtgatg
     7081 aggcggccct ggtaacgcac ttcaccttcc atgtgaagca gcagcccgca aatcgccttg
     7141 agcgtggtgg acttgcccgc gccgttacag ccaatcagcg cgacgagctc gccttcgccg
     7201 atgttgatat caatgccttt gaccgcattg atgccgccat aggagacctt gaggccgcgc
     7261 acttcaagga gattcatgcg agagcgcctc ccaaataagc ttcaattacc gccggatcgc
     7321 gctgtacctg ctgcggtgca ccttcggcga ttttcttgcc gtaatccagc accgccacgc
     7381 ggtcgcacaa gcccatcacc agcttcacgt cgtgctcaat cagaagaatc gtcgtgccgt
     7441 caccgcgtat ggtttccagc aaccttttca ggccgatagt ttcggttgcg ttcatcccgg
     7501 cggcaggctc gtcaagcgca agcaatttcg gctcggtggc aagcgcccgc gcgatttcca
     7561 gccggcgctg atcgccgtaa ctcagatgct tagcgagatc gttggcgcgt tgcgcgatgc
     7621 ccgcgtattc cagaagcgtt gaagcgcgcg agcggatcgc ttcctcttcc gcgcgcgttc
     7681 gggcatcgcg cagcaccgct ccaattactc cggcgcgggt gcgtacatgg cggccgacca
     7741 tgacgttttc cagcgccgtc atgttggcga agaggcggat gttctggaaa gtgcgcgcaa
     7801 taccttgttc tgcgaccgcg tgcggtttgg tggcggtgag ttttgcgccg ttgaagaaga
     7861 attcgccgtt gtccggcgca taaagaccgg tgagcacgtt gaaaaaagtg gttttgcccg
     7921 cgccgttggg gccgatcagt ccgtaaattt cccttctttc aataaatagg cttacctcgt
     7981 cgagcgcgag caggccgccg aagcgtttac tgactccgcg cgcttccagc aggttcatgc
     8041 gcgcgcctcg cttttcgcca tctcgttttc ggcggcaaac tcgcgcttgc gggtggtgga
     8101 aggccacaga ccagccgggc ggaacagcat gacgagaatc aacgccagtc cgaacaccag
     8161 catacgcaaa tccgctggat ccacgatgat gcggccgaac agcgcctgct cgaggggccc
     8221 gatgtagcgc aaggcttcgg gcagaatcgc cagcagcagc acgccgagaa tcacgccggg
     8281 aatatggccc atgccgccca gcaccaccat gcacagaatc gtgatggatt cgatgagagt
     8341 gaagctctcc ggactgatga agccctgaaa gccggcgaac aaaccgccgg aaacaccgcc
     8401 gaagctcgcg cccatggcga aggcgaggag cttcacgttg cgcgtgttga tgcccatcgc
     8461 tttcgccgcc acttcgtctt cgcggatggc gatccaggcg cggccgatgc gcgaatcctg
     8521 caagcgcatg gaaaagaaaa tgaccagcac ggtgagcgcg agaaataaat aatagtagtt
     8581 atgcaccgag gaaaaattga ttcccgccag ttgatgcgtc tgactgagag aaaaattgcc
     8641 caggcttacc ggatcaatcc ggttcacgcc ttgcgggccg ttggtgatgt tgaccggcgc
     8701 gttgagatta ttgagaaaga tacggatgat ttcgccgaag cccaaagtca cgatggcgag
     8761 gtaatcgccg cgcagccgca acgtcggcgc gcccagcagc acgccgaaaa tacaggtcac
     8821 accggcaccc aaaggcaata acacccagaa cggcagatgc aggccaaagt gcggcgaggc
     8881 aaggagcgca tagacataag cgcctaccgc gaaaaacgcc acgtagccca agtcgagcag
     8941 cccggcatag ccgacgacga tgttcaagcc caatgcaagc atgatgtaga gcagcgcgaa
     9001 atcgagaatg cgcacccagg tgcggccgag agccgcatcg gcgagaaacg gcagcaccgc
     9061 gagcaatacc gcgagcagga tgaacgctgc ccaagcggcg cgcggattgc gtttgagttt
     9121 ttcccagcgg ttcatttatg cccgctccgc gattcgctcg cccatcaagc ccgagggacg
     9181 gaacaccaga accagaatca gcacgaagaa cgcaaatacg tcctggtaat tgctgcccag
     9241 aaatccgccg gtgagcagtc cgatgtagcc cgcgcccaga ctctcgatga taccgagcaa
     9301 tagcccgccc agcattgccc cgccaaaatt gccgataccg cccagcactg ccgcggtaaa
     9361 cgccttgagc cccagagaaa agcccatgta atagtgcgca aggccataat agccgctcac
     9421 catcacgccc gccaccgcgg ccagcgccga gcccactaca aaagtaatcg agatgatggt
     9481 attgatattg acgcccatca atcccgcgac ttcggcgttt tgcgcggtag cgcgcatcgc
     9541 ccgccccagg cgcgtgcggt gcaccagaaa caacaacccc gccatgatta aagcggcaag
     9601 cagcacgatg aaaatctgca gatcggaaat agcggcgccg gcgatgaaat gaggcttgga
     9661 gggaagcagc ggaggaaagg tgatgtattg ccggccccag ataatcatcg cgctgttttg
     9721 cagcacgatg gacatgccga ttgcggtgat cagtggtgcg agccgcggcg cgttgcgcag
     9781 cgggcggtaa gcgatgcgct cgatgccgta acccaatacc atgcaagtcg gcatggcgac
     9841 cagcagtccc agcaaaacaa tcaccgggcc ggggagcgcg ctgccggcaa gcgcgtggat
     9901 cacggcgagc gccaccatcg cgcctatcat cactacttcg ccatgggcga agataatcag
     9961 accgaggatg ccgtagacca tggtgtagcc cagcgccacc aaggcataca cgctgcccag
    10021 cacaataccg ttgattattt gctggagaaa aatatccata gggctctata tacagcaaaa
    10081 cggcacccga aggtgccgca gcgtaagtat ggatttcgtt tagtagccct gacgcgtcga
    10141 gccggcggcg cttgccggag cggccgcgtc ttgctgcgcc ggcggcgctt cttgtcccag
    10201 cgtctgcagc ggcacccagt taccaccttt aacttggtat agggtcaccg cgccgccttt
    10261 gacgtcgcct ttggcatcga aggctatggg cccggtcacg ccgttgcgct gaatttgcgc
    10321 gagcaccgga agatatttgg ccggctccaa cgagtcggct tttttcatcg cctcaattaa
    10381 tgtcatcgtg gcgtcatacg cgtatggcgc gtaaatctgg attttgccgt atttcgattc
    10441 gaattttttt aggaagtcgc tcccgccggg cattttcccc agcggcaggc cgggcgagga
    10501 agcggtaacg ccttcagcgt tcgcgccggc gatcttgatg aattcattgc tttgtgtgcc
    10561 gtcaccgccg aggaatttgg cgttcagtcc cagcgatctc atctgcttca ccatcggccc
    10621 cgcttgcgcg tccatgccgc catagaaaac cagatccggc tgcttgcctt tgatggaggt
    10681 gaggatggcg agaaaatcgg tcgccctgtc ggtggtgtat tcgcgcgcta gaacctccgc
    10741 gccgccggcc tttgctgctt tctcgaactg gtcagccagc ccctggccgt aagcggtgcg
    10801 gtcgtcaatg atggcgattt ttttcgcggc catttggctc accgcgaatt caccgagcac
    10861 tttgccttgc tgttcatcgt tggtcatgac ccggaaggca ttcttgaagc cttgctgagt
    10921 gtagggaatc gcggtggcgg agggggaaat ctgcaccacg tcggcgtcgt ggtaaatctt
    10981 ggaagcggga atagtggcgc cggagttaag gtggccgatg accgcgtt
//
LOCUS       DATIOW010000040         9815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1337973, whole genome shotgun sequence.
ACCESSION   DATIOW010000040 DATIOW010000000
VERSION     DATIOW010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9815
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337973"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VLL03_01175"
     CDS             complement(<1..336)
                     /locus_tag="VLL03_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HSS46014.1"
                     /translation="MIRAVLFDLDGTLADTAPDLGFALNHQRKMHGLSALPLPTIRPY
                     VSQGGRGLMKIGFNLAPDDENYAAMREEFLDLYEQRLCHDTRLFPGISELLAELERKD
                     VPWAVVTNKI"
     gene            complement(333..1025)
                     /gene="ubiG"
                     /locus_tag="VLL03_01180"
     CDS             complement(333..1025)
                     /gene="ubiG"
                     /locus_tag="VLL03_01180"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HSS46015.1"
                     /translation="MNVDPAELQKFNELAHRWWDPGAEFKPLHDINPLRVDYIDKIAG
                     LKGKAVLDVGCGGGILAESMAVRGAKVAGIDLSEKALKVAQLHLMESGNQVDYRMVAV
                     EELARKEPQHYDIVTCMEFLEHVPGPASAVKACCELVKPGGHVFFSTLNRNLKSYLLA
                     IIGAEYILRLLPRGTHDYAKFIKPSELARFCRDAGLNVEHITGMTYNPFTKIYALGPD
                     TDVNYLLHAIKS"
     gene            complement(1030..2346)
                     /locus_tag="VLL03_01185"
     CDS             complement(1030..2346)
                     /locus_tag="VLL03_01185"
                     /inference="COORDINATES: protein motif:HMM:NF006549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HSS46016.1"
                     /translation="MPQHVDTLIDAGWVVPVEPRAVLRQHSLAIRDGMIQAILPTGKA
                     HAQFHANRIVRLENHALIPGLINLHTHAAMTLMRGLADDLPLMDWLNQHIWPAESKHV
                     SHPFVYDGTLLACAEMLRGGITCFNDMYFFPEAAAKAAVETGLRAAIGIIVIEFPSSY
                     AGDPGDYLNKGLAMRDEWRPQPLLSFCMAPHAPYTVSDKTFEKLLTYAQQLDLPINIH
                     VQETQEEIKQSLAQYKMRPLERLHKLGLLGPNFIAVHAVHLTAREIALLASEACSVAH
                     CPSSNLKLASGFAPVAALLEQGVNVGLGTDGAASNNRLDLFGEMRLAAMLAKTVSGKA
                     DALPAHQALQMATLNGAKALGLDGKIGSLKAGKAADITAMDFSKIELAPCFDPVSHLV
                     YAAGREQVSHVWVNGKLLLENRELTTLNARELTAKARYWQEKIGVA"
     gene            2570..5122
                     /gene="gyrA"
                     /locus_tag="VLL03_01190"
     CDS             2570..5122
                     /gene="gyrA"
                     /locus_tag="VLL03_01190"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004527513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HSS46017.1"
                     /translation="MDQFAKETLPVSLEEEMRRSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMHEVNNVWNRPYVKCARIVGDVLGKYHPHGDAATYEALVRMAQNFSMRYTL
                     IDGQGNFGSVDGDAPAAYRYTECRMARVSSEILSDIDKETVDFVPNYDGKEQEPVVLP
                     TKVPNLLVNGSSGIAVGMATNIPPHNLNEVVNACLALLNHPETSIDELIEMIPAPDFP
                     TAGIIYGVAGVREGYRTGRGKVVIRARTHFEEMDKGNRQLIVIDELPYQVNKAALLIR
                     IGELVRDKKLEGISDLRDESDKSGMRVAIELKRGENPEVVLNNLYKETQMQDTFGMNM
                     VALVDNQPRVLNLKNLLELFLRHRREVVTRRTVFELRKARERAHILEGLAVALSNVDK
                     VIELIKKAESPAVAKTQLMAHFWRSKLVEDMLHRASSGASRPEGLSPEFGLRKQGYYL
                     SDAQAQAILELRLQRLTGLEQEKIISEYKEVIEKITDLMDILAKRERITQIITGELNT
                     VKEQYGDQRKSEIVVDTQDLSMEDLIAPEDVVVTLSHGGYMKSQPVADYRAQKRGGRG
                     KQAAPTKEDDFIDSLFIANTHDYILCFSNRGRVYWLKVYNVPQGSRASRGKPIVNLVP
                     LMEHEKITAVLPIKEFDDEHFVFMATAKGTVKKTALSAFSRPMAKGIIAVNLEDGDYL
                     IGVALTNGGRDIMLFSDGGKAVRFGEKAVRVMGRNAAGVRGMKLTGDQRVISLLTAEN
                     ENQSVLTATENGYGKRTPIAEYTRHGRGTQGMIAIQTSARNGKVVVATLVDKEDEIML
                     ITTGGVLIRTRVSEIREMSRSTQGVTLINLGEGEKLAGLGKVAESDEESNDE"
     gene            5112..6224
                     /gene="serC"
                     /locus_tag="VLL03_01195"
     CDS             5112..6224
                     /gene="serC"
                     /locus_tag="VLL03_01195"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HSS46018.1"
                     /translation="MMNDVKGFTATKRVYNFGAGPAVLPEEVLEQARAEMLDWRGSGM
                     SVMEMSHRGKEFMSIAARAQSDLRELMQIPPHYKVLFLQGGATLQFAMAPLNLLRGKK
                     SADYIHTGEWSKKAIAEAKKFCAVNIAASSEEAKFTYAPAQEQWRLDPNAAYLHYTPN
                     ETINGVEFHWTPDTGDVPLVADMSSNILSKPVDVSQYGLIYAGAQKNIGPAGLTVVIV
                     REDLLGQPLPGTPAMLDYKIHADNESTYNTPPAYAWYVAGLTFQWLKKNGGLAAMESR
                     NIEKAGLLYDFLDHSSLFRNLVRKSDRSRMNVPFTLKQAVLDEEFLKQAKARGMIQLK
                     GHRSVGGMRASLYNAMPLEGVQTLVEFMREFEEQHG"
     gene            6217..7401
                     /locus_tag="VLL03_01200"
     CDS             6217..7401
                     /locus_tag="VLL03_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HSS46019.1"
                     /translation="MAETYHILTLDQISAQGLKRFPHNYVVGNHVSDPDAILVRSQNM
                     HNMTIPPKVKAIGRAGAGTNNIPVQQMNVRGVPVFNAPGANANAVKELVIAAMLMASR
                     NLIPAMRFVDTLQGDDEALDKKVEEGKKQFSGMELPHRSLGIIGLGAIGGMVADAAIK
                     LGMRVIGFDPEITVDAAWNVPSQVKKAHSIDEVLKNSDFVSLHVPLLDVTRNMIDAKR
                     VHNMRHGAILLNFARDGIVDEDALLAGLASKKIKYYMCDFPSEKLQGQHGVVALPHLG
                     ASTQEAEDNCAVMVVDQVRDYLENGNILNAMNFPNVVMMRESPYRVGIVNANVPNMVG
                     QISTTMAQAGLNIHNMMNKSKGEMAYTLLDVDSPAPPEVIGKIAAISGVLSVRNLPVQ
                     TE"
     gene            7419..8483
                     /gene="pheA"
                     /locus_tag="VLL03_01205"
     CDS             7419..8483
                     /gene="pheA"
                     /locus_tag="VLL03_01205"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HSS46020.1"
                     /translation="MSDELKKLRACIDTLDKQILELVNRRAAHARSIGRLKNGVIYRP
                     EREAQILRRIKKANHGPLSDETVARLFREIMSACLALEKPLTVTYLGPQGTFSEAAAI
                     KHFGHAANTLACVSIDEVLRKVEAGEVEYAVAPVENSTEGAVGRTLDLMLNTPLKICG
                     EVVLRVHQHLLRKSRGLKNIKRIYSHAQSFAQCHEWLNHNLHNVSWVQVASNAEAARL
                     AGKDASSVAIAGEMAAERYRLNTLAKNIEDEPNNTTRFWILGAHDAAPSGKDKTSLVM
                     SSKNVPGAVHQLLTPLAHYGVSMSKLESRPSRTGLWEYVFFVDVEGHQQEENVAKALD
                     ELADKAAFLKILGSYPTAVL"
     gene            8517..9617
                     /gene="hisC"
                     /locus_tag="VLL03_01210"
     CDS             8517..9617
                     /gene="hisC"
                     /locus_tag="VLL03_01210"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HSS46021.1"
                     /translation="MDLCDLAPPYIRAIAPYQPGKPISELERELGIKNIVKLASNENP
                     MGASPKALAAIKQALNDIARYPDGNGFELKAALSARLQVKQEQIVLGNGSNDVLELAA
                     RVFLTPGAEAVYAQHAFAVYPLATQACGARGIAAPAKNFGHDLEAMLKAVTAQTRLVF
                     IANPNNPTGTLLYADELLSFLRCLPETVLVVLDEAYNEYLSKELKTPSLEWLKQFPNL
                     VITRTFSKAYGLAGLRVGYALAQASVADLMNRVRQPFNVNSASQAAALAALDDHEFVK
                     RSFELNQKGMVQLTQAFKRLGLRYIPSYGNFVCFHADDAAALYQRLLRRGVIVRPIAN
                     YGMPEWLRVSIGLPHENEKFLKALESLMREAA"
     gene            9619..>9815
                     /locus_tag="VLL03_01215"
     CDS             9619..>9815
                     /locus_tag="VLL03_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HSS46022.1"
                     /translation="MNRRKLNKLVIFGVGLIGGSFALALKRAQAVRRIVGVGRSRDNL
                     KKALKLRVIDEIADSPAQAVQG"
BASE COUNT         2165 a         2721 c         2987 g         1942 t
ORIGIN      
        1 gattttgttg gtgaccaccg cccaagggac gtcttttctt tccagttcgg ctaagagctc
       61 ggaaattccg gggaagagcc gcgtgtcgtg acataaccgc tgttcgtaca aatccaaaaa
      121 ttcttcgcgc atggccgcat aattttcgtc gtcgggagcc aggttgaaac cgattttcat
      181 cagtccccgt ccgccctgcg agacataggg gcggatggtt ggcaaaggca gtgcggaaag
      241 tccatgcatt ttgcgctggt ggttgagcgc aaagccgaga tcgggagcag tgtcggcgag
      301 cgtgccgtca agatcaaaca gcacggcacg aatcatgatt tgatcgcgtg cagcagataa
      361 ttgacgtcgg tatccgggcc gagcgcatag attttagtga atgggttgta ggtcatgccg
      421 gtaatatgct cgacgttcag tcctgcatcg cggcagaagc gcgccagttc cgaaggcttg
      481 atgaatttgg cgtaatcatg cgtgccgcgc ggcagcagcc ttagaatata ttcggcgccg
      541 atgatggcga gaagatagga tttgaggttg cggttgaggg tggaaaaaaa tacatggccg
      601 cccggtttga cgagttcgca gcaggctttc accgcgctgg ccgggcccgg cacatgctcg
      661 aggaattcca tgcaggtcac tatgtcgtaa tgttgcggct ctttgcgcgc cagctcttcg
      721 acggccacca tgcggtaatc aacctggttg ccgctttcca tgagatgcag ctgcgccact
      781 ttcagcgctt tttcgctaag gtcgatgccg gcgactttgg cgccgcgtac cgccatgctt
      841 tccgcgagaa tcccgccgcc gcagccgaca tcaagcaccg ctttgccttt caacccggcg
      901 attttatcaa tgtagtcgac ccgcagcgga ttgatgtcgt gcaggggctt gaattcggct
      961 cccggatccc accagcggtg cgcgagctcg ttgaattttt gcagttctgc cgggtcgaca
     1021 ttcatgcttt tatgctacac cgattttttc ctgccagtag cgggccttcg cggtgagttc
     1081 cctggcattc agcgtggtca actcacggtt ttcgagtagc aacttgccgt tcacccacac
     1141 atggctgact tgctcccgtc ccgcggcata aaccaggtgc gataccgggt cgaagcaggg
     1201 tgcaagctca attttcgaaa aatccattgc tgtgatatca gcagctttgc cggctttcag
     1261 cgaaccgatt ttgccatcga gccccagcgc cttggcgccg tttaatgtcg ccatctgcag
     1321 tgcctgatga gccgggagcg catcggcttt tccgctcacc gtcttggcga gcattgccgc
     1381 aagccgcatt tccccgaaga gatccaggcg attgttgctc gccgcgccgt cggtgccaag
     1441 cccgacattg acgccttgct ccagcagcgc ggccaccggc gcgaaaccgc tggcgagctt
     1501 gagattggag gaggggcagt gcgcgacgct gcacgcttcg ctcgccagga gcgcaatttc
     1561 ccgggcagtc aaatgcacgg catgcacggc gatgaaattg gggccgagca atcccagctt
     1621 atgcagccgc tcaagcggcc gcatcttgta ttgcgccagg ctttgtttaa tctcttcctg
     1681 ggtttcctgt acatggatgt tgatcggtaa atcgagttgc tgcgcatagg tgaggagctt
     1741 ctcgaaggtc ttgtcgctta ccgtgtaggg cgcgtgtggc gccatgcaga aagaaagcag
     1801 cggttgcggg cgccattcat cgcgcatcgc caaccccttg ttcaagtaat ccccggggtc
     1861 gccggcgtag ctgctgggaa attcaatcac gatgatgccg atagcggcgc gcaggcccgt
     1921 ttccaccgcg gctttcgccg ccgcttcggg aaagaaatac atgtcgttga agcaggtgat
     1981 gccgccgcgc agcatttcgg cgcaggctag caatgtgccg tcgtaaacga acgggtgcga
     2041 gacgtgtttg ctctccgccg gccagatgtg ctggttcaac caatccatca gcggcaaatc
     2101 atcggcgagc ccgcgcatca gcgtcatcgc cgcgtgcgtg tgcaggttga tgagtcccgg
     2161 aatcagcgca tggttttcca gccggacaat ccggttggcg tgaaattgcg cgtgcgcttt
     2221 cccggtgggc agaatcgctt gaatcattcc atcgcggatg gcgagggaat gctggcgcaa
     2281 aactgcgcgc ggctcgaccg ggaccaccca gccggcgtca atcagggtat caacgtgttg
     2341 aggcatggcc tgtccaatga aaagccccgc accaagcagg gcttccattg taaccgcgaa
     2401 tgacgaactg ctgcagataa aggcattgca ccgccgggtt ttatacggct gagccgcgtt
     2461 tgcggagggt atgcgctact tggccttgac cctcggggca tgataaaatt tttaaatttg
     2521 atttgccgcg gttccagcta aaaaatacat cttaaaagcg cctgacttca tggaccaatt
     2581 cgccaaagaa accctgccgg tcagcctgga agaagagatg cgccgctcct acctcgatta
     2641 cgcgatgagc gtgattgtgg ggcgggcgct gccggatgtg cgcgacggct tgaagcccgt
     2701 gcaccgccgc gtgttgttcg ccatgcacga ggtgaacaac gtgtggaacc ggccgtatgt
     2761 gaagtgcgcg cgcatcgtcg gtgacgtgct cggcaaatat catccgcacg gcgacgcggc
     2821 cacttatgaa gcgctggtgc gcatggcgca aaacttttcc atgcgctaca cgctgatcga
     2881 tgggcagggc aacttcggct cagtggacgg cgacgcgcca gcggcgtacc gctacaccga
     2941 atgccgaatg gcgcgcgttt cctcggaaat cctttccgac atcgacaagg aaacggtgga
     3001 ctttgttccc aattacgacg gcaaggagca ggaacccgtc gttctgccga ccaaggttcc
     3061 caatttgctg gtcaacggct cctcgggcat cgcggtgggc atggcgacca acattccgcc
     3121 gcacaactta aacgaagtgg ttaacgcctg cttggcgctg ctcaaccatc cggagacgag
     3181 cattgacgag ctgattgaaa tgatcccggc tcccgatttt ccgaccgccg gtattattta
     3241 cggcgtcgcc ggcgtgcgcg aaggttaccg caccgggcgc ggcaaggtgg tgatccgcgc
     3301 ccgcacgcat ttcgaggaaa tggacaaggg caaccgccaa ttgatcgtaa ttgacgagct
     3361 gccataccag gtgaacaaag cggcgctctt gatccgcatc ggcgaactgg tgcgtgacaa
     3421 gaaactcgaa ggcatctctg atttgcgcga cgaatccgac aaatcgggca tgcgggtggc
     3481 gatcgaactc aagcgcggcg aaaaccccga agtcgtgctg aataacctgt acaaggaaac
     3541 acagatgcag gacacgttcg gcatgaacat ggtggcgctg gttgacaacc agccgcgcgt
     3601 gctgaatctc aagaacctgc tcgagctgtt tttgcgccat cgccgcgagg tggtgacgcg
     3661 gcgcacagtg ttcgaactgc gcaaggcgcg cgaacgggcg cacatcctcg aaggcctggc
     3721 ggtggcgctt tccaacgtgg acaaagtcat cgagctcatc aagaaggcgg aatcgccggc
     3781 ggtagcgaag acgcagttga tggcgcactt ttggcgctcg aagctcgtcg aagacatgct
     3841 gcaccgcgcc agctctggcg cttcgcgtcc cgaaggcctg tcgcccgaat tcgggctgcg
     3901 caaacagggg tattacctgt ctgatgcgca ggcgcaggcg attctggaat tgcgcctgca
     3961 gcgcctgacc ggtttggagc aggaaaaaat catttctgaa tacaaagagg ttatcgaaaa
     4021 aatcaccgac ctgatggata tcctggcgaa acgcgagcgt atcacccaga tcatcaccgg
     4081 ggaattgaat accgttaaag agcagtacgg cgaccagcgc aagagcgaaa tcgtggtgga
     4141 cacgcaggac ctgagcatgg aagacctgat cgcgccggag gatgtggtgg tgacgctgtc
     4201 gcacggcggc tacatgaaat cgcagccggt tgcggattac cgcgcgcaga agcgcggcgg
     4261 ccgcggcaag caagcggcgc ccaccaagga agacgatttc atcgactccc tgttcatcgc
     4321 caacacccac gactacatcc tgtgcttctc caaccgcggc cgagtgtact ggctcaaggt
     4381 ttacaacgtg ccacagggca gccgcgccag ccgcggcaag cccattgtca acctggtacc
     4441 gttgatggag cacgagaaaa tcaccgcggt gctgccgatc aaggaattcg atgacgagca
     4501 tttcgtattt atggcgaccg ccaagggcac ggtaaagaag accgcgcttt ccgcgttttc
     4561 gcggccgatg gcgaaaggca tcatcgccgt caacctcgaa gacggcgatt atctcatcgg
     4621 tgtagcgctc accaacggcg ggcgggacat catgctgttc tccgacggcg gcaaagcggt
     4681 gcgcttcggc gaaaaagcgg tgcgggtgat ggggcgcaac gcggcgggcg tgcgcggcat
     4741 gaagctcacc ggagaccagc gcgtcatttc gctcctgacc gcggagaatg agaaccagtc
     4801 ggtgctgacg gcgacggaga acggctacgg caagcgcact ccgattgccg aatacactcg
     4861 ccacggccgc ggcacgcagg gcatgatcgc cattcagacc tcggcgcgca acggcaaggt
     4921 ggtggtggcg acgctggttg acaaggaaga tgaaatcatg ctgattacca ccggcggcgt
     4981 gctgatccgc acccgcgttt cagaaattcg cgaaatgagc cgctccaccc aaggcgtgac
     5041 gctgatcaat ctcggggaag gcgaaaaact ggcgggattg ggaaaagtcg cggagagcga
     5101 cgaagaaagc aatgatgaat gatgtaaagg gatttaccgc gacaaaacgc gtgtataact
     5161 tcggagcggg accggcggtt ctgccagaag aggtgctgga gcaggcgcgc gcggaaatgc
     5221 tggactggcg cggcagcggc atgtcggtga tggagatgag ccaccgtggc aaggaattca
     5281 tgagcatcgc cgcccgggcg caaagcgatt tacgcgagct gatgcaaatc ccgccccact
     5341 ataaagtgct gttcctgcag ggcggtgcca cgctgcaatt cgccatggcg ccgctcaact
     5401 tgctgcgcgg gaaaaaatcc gccgactata tccataccgg tgaatggtcg aaaaaagcaa
     5461 ttgccgaggc gaaaaaattt tgcgcggtga atattgctgc ctcttccgag gaggccaaat
     5521 tcacttatgc accggcgcag gagcagtggc ggctcgatcc caatgccgct tacctgcatt
     5581 acacgccaaa tgaaactata aacggtgtgg aattccactg gacaccggat accggagacg
     5641 tcccgctggt cgccgacatg tcgtcgaaca ttctttccaa gccggtggac gtgagccaat
     5701 acggcctgat ttacgccggg gcgcaaaaga acatcggccc ggcgggcttg actgtggtga
     5761 tcgtgcgtga ggatttgctt ggccagccgc taccgggaac accggcgatg ctggattaca
     5821 aaatccacgc ggacaacgag tcgacgtaca acactccgcc agcctatgcg tggtacgtgg
     5881 cgggtctcac ttttcaatgg ctcaagaaaa acggcggtct tgccgcgatg gagagccgca
     5941 atatcgaaaa agccgggctg ctttacgact ttctcgatca cagcagcttg tttagaaatc
     6001 tggtgcgcaa aagcgaccgc tcgcgcatga acgtgccgtt cacgctcaag caagccgtgc
     6061 tggacgagga atttttgaaa caggcaaagg cgaggggaat gatacaactc aaggggcatc
     6121 gttccgtggg cggcatgcgt gcgtccctgt acaacgccat gccgctggaa ggcgtgcaaa
     6181 cgctggtgga attcatgcgc gagttcgagg agcagcatgg ctgagacgta tcacatcctc
     6241 acgcttgacc agatttcggc gcagggcttg aagcgttttc cgcacaatta tgtagttggc
     6301 aatcatgtga gcgatccgga cgcaatcctg gtgcgctcgc aaaacatgca caacatgacc
     6361 attccgccca aagtcaaggc gatagggcgc gccggagccg ggaccaacaa cattccggtc
     6421 cagcagatga atgtgcgcgg tgtgccggtg tttaacgcgc ctggggcgaa tgccaacgca
     6481 gtcaaagagc tggtgattgc agccatgctg atggcttcgc gcaacttgat tccggcgatg
     6541 cgtttcgtgg atacgctgca aggcgatgac gaagcattgg acaaaaaagt ggaggaaggg
     6601 aaaaagcaat tttcgggcat ggaactcccg caccgctcgc tcggcatcat cggcttgggc
     6661 gccatcggcg gcatggtcgc cgatgcggcg atcaagctcg gaatgcgtgt gattggtttt
     6721 gatcctgaaa ttaccgtgga cgcggcgtgg aacgtgccgt cacaggtgaa gaaggcgcac
     6781 agcatcgacg aggtgctgaa aaacagcgac ttcgtctcgc tgcacgtgcc gctcctggac
     6841 gtcacccgca acatgataga cgccaaacgc gtccacaaca tgcgccacgg cgctatcttg
     6901 ctgaatttcg cgcgcgatgg cattgtggac gaggacgcgc tgctggcggg gctggcctcc
     6961 aagaaaatca agtattacat gtgcgatttt ccgtccgaga aactgcaagg gcagcacggc
     7021 gtggtggcgt tgccgcatct gggcgcgtcc actcaggaag ccgaagacaa ttgtgcggta
     7081 atggtggtgg accaggtgcg ggactacctg gaaaacggca atatattgaa cgcgatgaat
     7141 ttccccaacg tagtgatgat gcgtgaatcg ccctaccgtg tgggaattgt caatgctaat
     7201 gtgccgaaca tggtggggca gatttccacc accatggcgc aggcggggct taacatccat
     7261 aacatgatga acaagtcaaa aggcgagatg gcttatactc tgctggatgt ggacagcccc
     7321 gcgccgcccg aggtcatcgg gaaaatcgcg gcgatttccg gcgtgctctc ggtgcgtaac
     7381 ctgccggtgc agacggaata acccaactaa tttagattat gagcgacgag cttaaaaaac
     7441 tgcgtgcctg catcgataca ctggataagc aaatcctcga gctcgtaaac cggcgcgccg
     7501 cgcacgcgcg ttccatcggc cggctcaaga acggcgtaat ttaccgcccg gagcgggaag
     7561 cgcagattct gcggcggatc aagaaagcga accatgggcc tctttccgac gaaacggtgg
     7621 cgcggctgtt ccgcgaaatc atgtctgcct gcctcgcgct ggaaaagccg cttaccgtga
     7681 cctacctcgg accgcagggc acgttcagcg aagccgccgc catcaagcat ttcggccatg
     7741 ccgccaatac cctggcgtgt gtttctatag acgaagtgct gcgcaaagtg gaggcggggg
     7801 aagtggagta cgccgtggcg ccggtggaaa actccaccga aggcgcggtc ggccgcacac
     7861 tggatttgat gctgaatacg ccgctcaaga tttgcggcga agtggtgttg cgcgtgcacc
     7921 agcatctgct gcgcaagagc agggggctta aaaacatcaa gcgtatctat tcgcacgcgc
     7981 aatcgttcgc gcaatgccac gagtggctca atcataatct gcacaacgtg tcatgggtgc
     8041 aggtggcgag caacgccgaa gccgcgcgcc tcgctggcaa ggacgccagc agcgtggcga
     8101 tcgccgggga aatggcggcg gagcgctatc gcctcaacac gctggcgaag aacatcgaag
     8161 acgagccgaa caacaccacg cgcttctgga tactgggcgc gcatgacgcc gcgccttcgg
     8221 gcaaggacaa gacttcgctg gtgatgtcca gcaaaaatgt tccgggagca gtgcaccagt
     8281 tgcttacgcc gcttgcccat tacggggtga gtatgagcaa gctggagtcg cgtccttcgc
     8341 gcaccggcct gtgggaatac gtgttcttcg tcgatgtcga aggccatcag caggaagaga
     8401 acgtggccaa ggcgctcgac gaactcgccg ataaggcagc gttcttaaaa atcctcggct
     8461 cctatcccac ggcggtgctt tgagctttgc aaagcatctc atttggaatg atccctatgg
     8521 atttatgtga ccttgcgccg ccctatatcc gggcgattgc gccttaccag ccgggcaagc
     8581 cgatttccga gctcgagcgc gagcttggaa ttaagaatat cgtgaagctc gcgtccaacg
     8641 aaaatcccat gggcgcgagc cccaaggcgc tcgctgcgat aaagcaggcg ctcaatgaca
     8701 ttgcccgcta tcccgatggc aacggttttg aattgaaagc ggcgttatct gcgcggctgc
     8761 aggtgaagca ggagcaaatc gtgctgggca acggctcgaa cgacgtgctg gaactcgctg
     8821 cgcgcgtttt tctcactccc ggcgccgagg cggtttacgc gcagcacgcg tttgccgtgt
     8881 atccgctcgc gacccaggcc tgcggcgcca ggggcatcgc agcgccggcg aaaaacttcg
     8941 gccatgattt ggaagccatg ctcaaggcgg taacggcgca gacgcgcctc gtgtttattg
     9001 ccaaccccaa caacccgacg gggaccctgc tttacgccga tgagttgctt agctttctgc
     9061 gttgcttgcc ggaaaccgtg ctggtggtat tggatgaagc gtataacgag tatttgtcaa
     9121 aagaactcaa gacacccagc ttagaatggc tgaaacaatt tccgaatctg gtgattacgc
     9181 gcacgttctc caaagcctat ggcttggcgg gtctgcgcgt gggctacgcg ctcgcgcagg
     9241 cttccgtcgc cgatttaatg aaccgcgtgc gtcagccgtt caacgtcaac agcgcttcgc
     9301 aagctgcggc actggcggcg ctcgatgacc atgagttcgt gaaacgaagc ttcgagctca
     9361 atcaaaaagg catggtacag ctcacgcagg ctttcaagcg tctggggctg cgttatattc
     9421 cttcgtatgg aaattttgtg tgcttccacg cggacgatgc ggcggcactg tatcagcgcc
     9481 tgctcaggcg cggcgtaatc gtgcggccga tcgccaatta cggcatgccg gaatggctgc
     9541 gagtaagcat aggcttgccg catgaaaacg aaaaattcct taaagctttg gaaagcctga
     9601 tgcgcgaagc ggcatgacat gaacaggcgt aaattaaaca agctggtgat ttttggcgtc
     9661 ggtctcatcg gcggctcgtt tgccctggcg ttgaagcgtg cgcaggcggt gaggcgtatc
     9721 gtcggcgtcg gacgcagtcg ggataacctg aaaaaagcac tgaagctgcg cgtgattgat
     9781 gaaatcgccg acagtccggc gcaagcggtg caggg
//
LOCUS       DATIOW010000041         7635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_321484, whole genome shotgun sequence.
ACCESSION   DATIOW010000041 DATIOW010000000
VERSION     DATIOW010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7635
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321484"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VLL03_01220"
     CDS             complement(<1..608)
                     /locus_tag="VLL03_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS46023.1"
                     /translation="MMKSAWCLFLSLLALGLPAATLAQTEPPLTHDQALQELDRPYAE
                     SRRSAVKRLGEIGLMSDTPALIKRLRDADRLTRGLAENALWQVWTRSGDEKVDALFRL
                     GVEQMQEGPGHEAIATFSKIIELKPDFAEGWNKRATLYFIMGKYRKSLADCGEVLKRN
                     PHHFGALAGYGQIYLRLNRPELALDYFRRALDINPNLRGVEI"
     gene            788..1213
                     /gene="ndk"
                     /locus_tag="VLL03_01225"
     CDS             788..1213
                     /gene="ndk"
                     /locus_tag="VLL03_01225"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HSS46024.1"
                     /translation="MAVERTLSIVKPDAVAKNIIGKIYTRFEEKGLKIVAARMLRLSR
                     IEAEGFYAVHRGRPFFKDLVAFMTSGPVMVQVLEGDNAITLNREIMGATDPKKAAKST
                     IRADFAQSLNFNAVHGSDSPHTAAMEIAYFFPTLDIHSR"
     gene            1213..2409
                     /gene="rlmN"
                     /locus_tag="VLL03_01230"
     CDS             1213..2409
                     /gene="rlmN"
                     /locus_tag="VLL03_01230"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HSS46025.1"
                     /translation="MLVNLLDYDLPQLAAFCSEIGERPFRARQLLRWIHHFGESDFTK
                     MSDLAKSLREKLSATAVIEPPKVKSDSMSADGTRKWLLEVGSGNAVEAVYIPEGDRGV
                     WNWGSACGAAMERETSDPAAASAPLVRPSRRYRGTLCISTQAGCALECTFCSTGRQGF
                     NRNLSVGEIIGQLWWANKALGHDPKGERVISNVVLMGMGEPLLNFDNVVTALRLMLDD
                     HAYGLSRRRVTLSTSGVVPAIERLREACPVALAVSLHAPNDRLRDMLVPINRKYPLRE
                     LLAACRRYITAAPRNFITFEYVMLEGVNDSVAQARELVTLVRNVPCKFNLIPFNSFPD
                     SGYRRSSPQTIVRFRDVLVEAGLVVTTRKTRGDDIDAACGQLAGEVQDRTKRKLRRSA
                     AMGDSP"
     gene            2406..3191
                     /gene="pilW"
                     /locus_tag="VLL03_01235"
     CDS             2406..3191
                     /gene="pilW"
                     /locus_tag="VLL03_01235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HSS46026.1"
                     /translation="MKKILLLGLAAMLAGCVQERVQTTSTSPLEAPPAKQADAKRRAQ
                     IHTELGSGYYERGQLGVALEELKEALRADSDYAQAYNVLGLVYMELREDADAERNFRQ
                     ALKLAPLDSDTNNNYGWFLCQRKREQESIKYFLAALKNPLYATPEKSYANAGICARRN
                     SDEAAAEAYFLKALRLRPDQQPALYNMADLKFKRGQHGEAKSYLVKYMQVTSVPTPES
                     LWLGVRIERKLGDSNAEASYAAQLRKRYPDSRETRTLNYGQFE"
     gene            3188..4015
                     /locus_tag="VLL03_01240"
     CDS             3188..4015
                     /locus_tag="VLL03_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HSS46027.1"
                     /translation="MTEGAADNQTASIGRVLSAARERQGLSVSDVARQLKLAVRQVEA
                     LEADDFKSLPRITFVRGFIRNYAKLVQVDVEPLLKAAKLMPPPPQVPEAERGVAEIPT
                     ASDKKYDWRSYAISGVLATLLAGFFAYEWYHGKPEKNVPKPANVKREPAPVEKSKTET
                     PQKPITEPVPAPRDLAQSSAEAAASQTDARPVAPRERGGEIRLEFVEDTWVEIADKSG
                     KTIFSKLNPAGSTQSVRGVPPFSVVVGNAAGVRIIYNDKPVDLKPYTKVDVARLTLK"
     gene            4075..5337
                     /gene="hisS"
                     /locus_tag="VLL03_01245"
     CDS             4075..5337
                     /gene="hisS"
                     /locus_tag="VLL03_01245"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HSS46028.1"
                     /translation="MKQNNIQAVRGMNDILPEQAHRWGAFEDTVREWLAAYGYRNIRM
                     PMVEKTDLFVRSIGEATDIVEKEMYTFVDRLNSESLTLRPEGTASCVRAVLEHNLLYP
                     GPLRLYYTGPMFRHERPQQGRYRQFHQIGVEALGFAGPDIDAEHLVMCARLWKKLGLS
                     GIKLQINSLGGAECRSRYRERLKKYLTSVAGQFDQKMKARVERNPLRVLDSKEPKMQP
                     VIEAAPRLIDELDAASLKHFEELQTLLKDAGIAFEINPRLVRGLDYYNLTVYEWVTDR
                     LGAQGTICAGGRYDGLVAQLGGGPAPACGYAMGIERLLAQQEREEKQPAPDAYLVHQG
                     ELADRQAFQIAEALRDHGLSVLLHCGGGSFKVQMKKADASGARYAVIIGDDEARADEV
                     SLKPLREAGEQIRSGLTRIIEKLRKPDV"
     gene            5330..5959
                     /locus_tag="VLL03_01250"
     CDS             5330..5959
                     /locus_tag="VLL03_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS46029.1"
                     /translation="MYDLQEQEQLDELKAWWKENGRRVITIVVVFALGAAAVQGWRHY
                     QQTQARQAAALYGTLEELLPGKDAKKIQEMAGAIVEKFPSTAYATRAALIAAKTNYDS
                     GDVKSAKAQLQWVIDHASEDELRDMARLRLGGALLDEKNYAEALSLLEAKHSPAYDPL
                     FYDLKGDILVAQGKTAEARDAYQTALDKSDKKSNYRSLIQVKLDALGGK"
     gene            5987..7120
                     /gene="bamB"
                     /locus_tag="VLL03_01255"
     CDS             5987..7120
                     /gene="bamB"
                     /locus_tag="VLL03_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HSS46030.1"
                     /translation="MPLLVLFLCACSDFLGFGSDPDTAEKPARLVTFTRTAKTAVKWQ
                     KNIGPSGEYVFAPVFAADSVYAANDEGELMRYDAMSGKLIWRVDTKRRLSGGVGAEHN
                     IVLVGDLKGELLAFDSEGYLQWKSQLTSEILSPARVADGIVVVRTEDGRIFGLDAETG
                     KRKWVYQRSTPALSVRSFAGVAITRGGVFAGFAGGKLVAIDLGTGNIGWEATVALPRG
                     TTELERVTDITSAPVVDQERVCAVAYQGRVACFDLLRGNLLWSREISSIAGMTLDERN
                     VYVSDDKGAVIALDKFSGASVWKQDKLFGRKLTAPLVFGRYVVVSDFHGYVHFLSRED
                     GAFAARLPTDGSRVFAPPIALPLSLLVQTHGGRLFAMTIRSNP"
     gene            7160..>7635
                     /locus_tag="VLL03_01260"
     CDS             7160..>7635
                     /locus_tag="VLL03_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HSS46031.1"
                     /translation="MKPTLVLVGRPNVGKSTLFNRLTKSRNAIVANVPGLTRDRHYGQ
                     GRIGTRPFLVVDTGGLEPAARQGILPEMAKQTLQAVAEGDCVLFLVDAREGLTVTDRM
                     IADNLRKCGRPLYLVVNKAEGMAPALAASEFHELGLGEPLPIAAAHGEGVNELVEL"
BASE COUNT         1627 a         2125 c         2405 g         1478 t
ORIGIN      
        1 ttgatttcaa caccacgcag gttgggattg atgtcgagcg cgcggcggaa ataatccagc
       61 gcgagttccg gccggttgag acgcaaataa atctggccgt agcccgccag cgcgccgaaa
      121 tgatggggat tgcgtttcaa cacctcgccg caatcggcta gcgatttccg gtatttcccc
      181 atgatgaagt aaagcgtggc gcgcttgttc cagccttcgg cgaaatccgg cttgagctcg
      241 ataatcttgc tgaaagtggc gatggcctcg tgccccggtc cttcctgcat ttgctcgacg
      301 ccgaggcgaa acagggcatc cactttttca tcgccagaac gagtccacac ttgccacagg
      361 gcattttcgg cgagcccgcg ggtgagccga tcggcatcgc gcaaccgctt gatcagcgcc
      421 ggcgtgtcgc tcatcaagcc gatttcaccc agtcgtttca ctgcgctgcg ccggctttcg
      481 gcatagggcc ggtcgagttc ctgcaacgcc tgatcgtgag tcagcggcgg ttcggtttgc
      541 gccagcgtgg cagcgggcaa ccctaacgcc agcagactca aaaacaagca ccaggcggat
      601 ttcatcatta tggcaaggcc gggacgtgtt cctcgtgcat tatgcgtgga tgggcgaatt
      661 ttgactatcg accttgtaaa aactgcgaaa aacccggccc ccaggcttta tttgaaggct
      721 ttatcatggt atttttacaa gttaatcaat ttattaaccc ttgcagaatc aactctggag
      781 attgtgaatg gctgttgaac gcaccctgtc catcgtcaag ccggatgccg tggcaaaaaa
      841 catcatcgga aaaatctaca cccgattcga ggaaaagggc ctcaaaatcg tcgccgcgcg
      901 catgctgagg ctgtcgcgca ttgaagcgga aggattttat gccgtgcacc gtggacggcc
      961 gtttttcaag gacctggtgg ctttcatgac ttccggcccg gtgatggtgc aggtgctgga
     1021 aggcgacaat gcgatcacgc tcaatcgtga aatcatgggc gcgaccgatc cgaagaaggc
     1081 ggcgaaaagc accatccgcg ccgatttcgc ccaaagcctc aacttcaatg cggtgcacgg
     1141 ctccgacagc ccgcataccg cggcgatgga aatcgcttat ttctttccga ccctggatat
     1201 acactcacgc tgatgctggt taatttgctc gattacgatc tgccacaact cgccgcattc
     1261 tgcagtgaaa tcggcgagcg gccgtttcgc gccagacagc ttttgcgctg gattcatcat
     1321 ttcggagaaa gcgatttcac caaaatgagc gatctcgcca agagcttgcg cgagaaactt
     1381 tcggcgactg cggtaattga gccgccgaaa gtaaaatcgg acagcatgtc cgccgacggc
     1441 acccgcaagt ggctgctgga agtgggcagc ggtaatgcgg tggaagcggt gtatattccg
     1501 gaaggcgacc gcggagtgtg gaactggggt tcggcttgcg gggcggccat ggagcgggag
     1561 acaagcgacc cggcggcggc cagcgcccct ttggtccggc cttcaaggcg ctaccgcggc
     1621 acgttgtgta tatcgacgca agcgggctgc gcgctggaat gcactttttg ttcgacgggc
     1681 aggcagggat tcaatcgcaa cttgagcgtg ggtgaaatca tcggccagtt gtggtgggcg
     1741 aacaaggcgc tgggtcatga tcccaagggc gagcgggtga tcagcaatgt ggtgttgatg
     1801 ggcatgggcg agccgctttt gaattttgac aacgtggtga ctgcgctgcg tttgatgctc
     1861 gatgaccatg cttacgggtt gtcgcgccgg cgcgtgacct tgagtacttc cggtgtggtg
     1921 ccagccatcg agcgcctgcg ggaagcctgc ccggtggcgc tcgcggtgtc gctgcatgca
     1981 cccaacgacc ggctgcggga catgctggtg ccgattaaca ggaaatatcc gctgcgcgaa
     2041 ttgctcgctg cctgccgaag atatattact gccgcaccga ggaatttcat caccttcgaa
     2101 tatgtgatgc tggaaggggt gaacgacagc gtggcccaag cgcgcgaact ggtaactctg
     2161 gtcaggaacg tgccgtgcaa attcaattta atccccttca attcatttcc cgattccggt
     2221 tatcggcgtt cctcgcccca aaccattgtg cgtttccgcg acgtgctggt ggaagccggt
     2281 ctggtggtca ccacgcgcaa gacgcgcggc gacgacattg acgcggcatg tgggcaactg
     2341 gccggcgaag tgcaggatcg caccaagcgc aaattgcgcc gtagcgccgc gatgggagat
     2401 tcaccgtgaa aaaaatcctt ttgcttggat tggcggccat gcttgccggc tgcgttcagg
     2461 agcgggtgca aaccacgtcc acttcgccgc tggaagcgcc gccggccaaa caggcggatg
     2521 caaaacgccg cgcgcaaatc catactgaac tgggctcggg atattatgag cgcggccagc
     2581 tgggcgtggc gttggaagaa ttaaaggagg ctttgcgcgc cgattccgat tatgcgcagg
     2641 cgtacaacgt gctggggctg gtatacatgg agttgcgtga agatgcggac gcggagcgca
     2701 atttccggca ggcgctcaag ctcgcgccgc tggattcgga cacgaacaat aattacggct
     2761 ggtttttgtg ccagcgcaag cgcgagcagg agtcaatcaa gtatttcctc gcggcgctga
     2821 aaaatccgct ctacgccacg ccggaaaaat cctatgccaa tgccggtatt tgcgcgcgcc
     2881 gcaatagcga tgaggcggcc gccgaggcct atttcctgaa agcgctgcgc ttgcgccccg
     2941 atcagcaacc ggcgctttat aacatggccg acctcaagtt caaacgcggc cagcacggcg
     3001 aagccaaatc atatctggtc aaatacatgc aggtgacgtc ggtgccaacc ccggaaagcc
     3061 tgtggctggg cgtgcgcatt gagcgcaaac tgggcgacag caatgcggaa gcaagctacg
     3121 ccgcgcaatt gcgcaagcgt tatccggact ccagggaaac ccgcacactg aattacgggc
     3181 agttcgaatg actgaaggcg ctgcggacaa ccaaacggca agcatcggcc gcgttctatc
     3241 cgcggcgcgt gagcgccagg gcctgagcgt gtctgatgtg gcgcgtcagc tcaaactcgc
     3301 cgtgcgccag gtggaagcgc tggaagcgga tgattttaag agcctgccgc gcatcacgtt
     3361 cgtgcgcggc tttatccgca actacgcgaa gctggtgcaa gttgacgtcg agccgctgct
     3421 caaggccgcc aaactgatgc cgccgccgcc gcaagtgcct gaagccgagc ggggcgtggc
     3481 ggaaatcccc accgcttccg acaaaaaata cgactggcgc agttacgcga tatccggcgt
     3541 gttggctacg ctgctggcag gtttctttgc ttatgagtgg tatcacggca agccggaaaa
     3601 gaatgtgccc aagccggcca atgtaaaacg cgaacccgca ccggtggaga aaagcaaaac
     3661 cgagacaccg cagaagccaa taacggagcc ggtgcccgcc ccacgcgatc tggcgcaaag
     3721 cagcgcggaa gcagcggcat cgcaaacgga tgccaggccg gttgcgccgc gcgaacgggg
     3781 cggggagatt cgcctggagt tcgtggagga tacatgggtg gaaattgccg acaagagcgg
     3841 caagacgatt ttttccaagc tcaatccggc gggcagcacg cagtcggtgc gcggcgtgcc
     3901 gccgttttcg gtggtggtcg gtaacgccgc aggggtgagg attatttaca atgacaagcc
     3961 ggtggatttg aagccgtata ccaaagtcga tgtggcgcgc ctgactttga agtagcgccc
     4021 ggagccccgg actttgttaa tggggcggcc gatgggcctc ctctcttcag aacaatgaaa
     4081 caaaataaca ttcaggcggt acgcggtatg aacgacatcc tgccggagca ggcgcaccgg
     4141 tggggggcgt tcgaagacac ggtgcgcgag tggctggccg cctacggcta tcgcaacatc
     4201 cgcatgccaa tggttgaaaa gaccgatctg ttcgtgcgct cgattggcga agcgaccgat
     4261 atcgtcgaga aggagatgta caccttcgtc gaccggttaa acagcgagag cctcaccttg
     4321 cggccggaag gcaccgcttc ctgcgtgcgc gcggtgctcg agcataatct gttgtatccc
     4381 gggccgctgc gtctttatta tacgggcccg atgttccgcc acgagcgccc gcagcagggg
     4441 cgttaccgcc agttccacca gatcggggtg gaggcgttgg gttttgccgg gcccgatatc
     4501 gacgccgagc acctcgtgat gtgcgcgcgg ctgtggaaaa aactgggttt atcgggcatc
     4561 aagctacaaa tcaattcgct gggcggcgcg gagtgccgca gccgctaccg cgagcggctg
     4621 aaaaaatatc tgacatcggt tgccggccag tttgaccaaa agatgaaagc gcgcgtggag
     4681 agaaacccgc tgcgcgtact cgacagcaag gagccgaaga tgcagcctgt catcgaggcc
     4741 gcgccgcggc tcatagatga gctggacgcg gcttcactca agcactttga agagctgcag
     4801 acgctgctca aggacgcggg cattgctttc gaaatcaatc cacgcctggt gcgcgggctc
     4861 gattactaca acctgacggt gtacgagtgg gtcaccgaca ggctgggtgc gcaaggcacc
     4921 atctgtgctg gcgggcgcta cgacggattg gttgcgcaat tgggcggcgg gcccgcgccg
     4981 gcgtgcggct acgccatggg catcgagcgc ctgctagcgc agcaagagcg ggaagaaaag
     5041 caacccgcgc ccgatgctta tctggtgcat cagggcgaac ttgccgaccg ccaggcattc
     5101 caaattgcgg aagccctgcg cgaccatggc ttgagcgtgc tcctgcattg cggcggcggc
     5161 agtttcaagg tgcaaatgaa aaaagcggat gccagcggcg cgcgttacgc cgtgattatc
     5221 ggcgatgacg aagcgcgggc cgatgaagtg agcctgaaac ccttgcgcga agcgggcgaa
     5281 caaatccgct caggattgac acggattatc gagaaactga ggaaacccga tgtatgactt
     5341 gcaggaacaa gaacagctag acgagcttaa agcctggtgg aaggaaaacg gcaggcgggt
     5401 gattaccatc gtggtggttt tcgccttggg cgcggcggca gtgcaaggct ggcgccatta
     5461 ccagcaaact caggcccggc aggcggccgc actttatggg acgctggagg aactgctgcc
     5521 aggcaaggac gccaaaaaaa ttcaggagat ggcaggcgcc atcgtcgaaa aatttccatc
     5581 caccgcttat gccacgcgcg ctgcattgat tgccgccaag accaattatg attcgggcga
     5641 tgttaaaagc gccaaggccc agctgcaatg ggtcattgac cacgcttccg aagacgagct
     5701 tcgggacatg gcgaggctgc ggcttggcgg cgcgctgctc gatgaaaaaa attatgccga
     5761 ggccctttcg ctcctcgagg ccaaacacag cccggcctat gacccgctgt tttatgatct
     5821 caagggagac atcctggttg cccaaggcaa aaccgccgag gcaagggacg cctaccagac
     5881 ggcgctcgac aaaagcgaca agaaaagcaa ttaccgcagc ctgattcagg tcaagctgga
     5941 cgctctggga ggaaagtaaa tgaagcggcg gttttcccgg gcggtcatgc cgcttcttgt
     6001 acttttcctt tgcgcctgct ccgatttcct cggttttggc agtgatccgg ataccgcgga
     6061 gaagccggcg cggttggtga cgtttacgcg caccgccaaa accgcagtca agtggcaaaa
     6121 gaacatcggc ccgtccggcg agtacgtatt cgccccggtg ttcgccgccg acagcgttta
     6181 cgccgccaac gatgagggcg aactgatgcg ctacgacgcc atgagcggaa agctgatttg
     6241 gcgggtggac accaagcgca ggctatccgg gggcgtcggc gccgagcaca atatagtgct
     6301 ggtaggcgat ttgaaaggcg agttgctggc gtttgatagt gagggttatc ttcagtggaa
     6361 atcgcaactc accagcgaaa tcctgagccc cgcccgcgtg gcggacggaa tcgtggtggt
     6421 gcgcactgaa gacgggcgga ttttcgggct ggacgcggaa accggcaagc gcaagtgggt
     6481 ctaccagcgt agcaccccag cgcttagcgt gcgttcgttt gccggcgtgg cgattacgcg
     6541 cggcggggta tttgccggat ttgctggcgg caagctggtc gcgatcgacc ttggcaccgg
     6601 aaatatcggc tgggaagcga cggtggcgct gccgcgcggc accacagaat tggagcgtgt
     6661 caccgacatt accagcgctc cggtggtgga tcaggaacgc gtttgcgcgg tggcgtacca
     6721 gggacgagtg gcgtgttttg acctgttgcg cggcaatctt ctctggtcgc gcgaaatctc
     6781 gagcatcgcc ggcatgaccc tggacgagcg caatgtctat gtgagcgacg acaagggcgc
     6841 ggtgattgca ctggacaagt ttagcggcgc cagcgtgtgg aaacaagaca agcttttcgg
     6901 gcgcaagctt accgcaccgc tggtgtttgg ccgctatgtg gttgtttccg attttcacgg
     6961 ttatgtgcat ttcctgtcgc gtgaagacgg cgcctttgcc gcgcgtcttc ccaccgatgg
     7021 cagccgcgtc ttcgcgccgc cgattgcgct gccgctaagt cttttggtgc agactcacgg
     7081 cggcaggctg ttcgccatga cgatccgatc caatccgtga aggttaatcg gacgaacccg
     7141 ctaccgctta tagtgcatca tgaaacccac cctggtcttg gtcggccgcc ccaatgtcgg
     7201 caaatccacg ctgtttaacc ggctcaccaa aagccgtaat gccatcgtcg ccaacgtccc
     7261 tggcctgacc cgcgaccgcc attatgggca gggcagaatc ggaactcggc cgtttctggt
     7321 agtggatacc ggcggcctcg agccggcggc caggcagggc attttgccgg aaatggcgaa
     7381 acaaacattg caagcggtgg cggaaggcga ctgcgttctg tttctggtcg atgcgcggga
     7441 aggattgacg gtcacggacc ggatgattgc cgataatctt cgcaaatgcg ggcgccccct
     7501 ctatctggtg gtgaacaagg cggaaggaat ggcgcccgct ctagccgcat ccgaatttca
     7561 cgagctgggt ttgggcgaac ccttgccgat agcggcggcg catggcgaag gcgtgaacga
     7621 gctcgtcgag ctgat
//
LOCUS       DATIOW010000042         6312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1236144, whole genome shotgun sequence.
ACCESSION   DATIOW010000042 DATIOW010000000
VERSION     DATIOW010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236144"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /gene="ychF"
                     /locus_tag="VLL03_01265"
     CDS             complement(<1..830)
                     /gene="ychF"
                     /locus_tag="VLL03_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HSS46032.1"
                     /translation="MSLRCGIVGLPNVGKSTLFNALTKAGVAAENYPFCTVDPNVGVV
                     EVPDPRLKATADIAKPQKVQPALTEFVDIAGLVAGASKGEGLGNQFLGHIRETDGIVH
                     VVRCFEKENVVHVAGRIDPVSDIEVVNTELALADLATVGKQYARYAKAAKAGADKEAQ
                     RVIAVLDKIRPALDQARLARSVPLAREEQAVLQPLFLLTAKPTMYVANVAEHGFENNP
                     LLDKVRAHAKSENAPVLAICAELEAEIADLSDEDKKVFLADMGMQEPGLNRLIRAAFD
                     "
     gene            complement(827..1411)
                     /gene="pth"
                     /locus_tag="VLL03_01270"
     CDS             complement(827..1411)
                     /gene="pth"
                     /locus_tag="VLL03_01270"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HSS46033.1"
                     /translation="MHNVRLVVGLGNPGKEHAADRHNAGYRWLAHLAKEKRVLLKLEA
                     RFHALMGKLARDSEECWLMQPQTFMNASGRAVGALAKFYKIPAQEILVVHDELDLPPG
                     VARLKQGGGVSGHNGLKDIAAHLGSPDFWRLRIGIGHPGEKSLVVDYVLDHPRAEEKK
                     LIEQAIHQSLEVWPLIAEGKLQAAMHKLHTKQDK"
     gene            complement(1614..2255)
                     /locus_tag="VLL03_01275"
                     /pseudo
     CDS             complement(1614..2255)
                     /locus_tag="VLL03_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633417.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
     gene            complement(2322..3272)
                     /locus_tag="VLL03_01280"
     CDS             complement(2322..3272)
                     /locus_tag="VLL03_01280"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HSS46034.1"
                     /translation="MAYDSLMVFTGNANPKLAEQVVKHLNIHLGRATVGRFSDGEVMV
                     EILENVRGKDVFVLQSTCSPPNESLIELLVMVDALKRASAGRITAAIPYFGYARQDRR
                     PRSARVPITAKMVANMLTSVGVDRLLIMDLHSEQIQGFFDIPVDNIYAMPILLGDIWK
                     HDHQNLVVVSPDVGGVVRARAAATPLEADLAIIDKRRPRPNVAKVMNIIGEVKGRTCI
                     IMDDMVDTANTLCEAAEALKREGAEKVLAYCTHPVLSGKAVERIENSVLDELVVTNTI
                     PLSEAATKCKRIRCLCVAELLGETMLRISNEDSVSSLFME"
     gene            complement(3323..3397)
                     /locus_tag="VLL03_01285"
     tRNA            complement(3323..3397)
                     /locus_tag="VLL03_01285"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3363..3365),aa:Gln,seq:ttg)
     gene            complement(3401..4240)
                     /gene="ispE"
                     /locus_tag="VLL03_01290"
     CDS             complement(3401..4240)
                     /gene="ispE"
                     /locus_tag="VLL03_01290"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HSS46035.1"
                     /translation="MSDLQTYPAPAKLNLFLHVVGRREDGYHLLQSVFRFLDYADSLS
                     FRVRDDGVIKRGYALPGVPEGDDLCAQAAKLLQRTAGTRLGVDITLQKRLPLGGGLGG
                     GSSDAATTLMALNRLWKLNFSCARLQQLGLKLGADVPVFVFGQSAFAEGVGEVLQPIS
                     LPPAWYVVLVPKVNVSTAEIFADPELTRNTNRIKITAFSTGLGHNDLEPVVCRKYPQV
                     AQHLAWLKQFGAARMTGSGACVFAEYATEGEARVVLAQLPGQMSGFVARGLDRHPLLD
                     LTL"
     gene            complement(4237..4854)
                     /gene="lolB"
                     /locus_tag="VLL03_01295"
     CDS             complement(4237..4854)
                     /gene="lolB"
                     /locus_tag="VLL03_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HSS46036.1"
                     /translation="MIRNLVRGSRVYWLALLLLLGACAAHPPKPVSPALSRAQSLVAA
                     QRNGFQLSGRVAVKYDEKNFSGSVRWEHNGSSDEIWILAPLGSGVAHIVQNANGISLT
                     TNEQKTYRAFDAESLTRAVLGWSLPLSGLQYWILGIAAPQSAAQAQHDAQQRLARLNQ
                     DSWEIAYLRYKPEQESGMPARIILSRGGLQINLIIDDWIISPLAQ"
     gene            complement(4851..>6312)
                     /locus_tag="VLL03_01300"
     CDS             complement(4851..>6312)
                     /locus_tag="VLL03_01300"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS46037.1"
                     /translation="QRATEIALFSRQPEAALQAARIWVEVDPESTPARQTLVALLING
                     RKLDEARPNIEKLLSAEGKSVGQGFMQLNGLLSKYPDKQAALNLVQELAQPYPRVAEA
                     HLAIAQAAWNAMQYDVALSEVRKVLELRPEWELAALFQGQTLQHTSNKLALEYLRGYL
                     KSYPKAKDVRLNYARLLVAEKDYPQARSEFQKLRAAFPNNAEVSLAVGLLSMQLNDFD
                     AAEENFKHALSQNYKDRDSVRFYLGQIYEERRRYDEALRWYGSVGEGEHYLNARTRYA
                     GVLAKQGKLAEARKYLGQIAVQDNQQRIQILLTEAQLLREAAHYREAFDLLGRALEKN
                     PDYPDLLYDYAMAAEKLNRLDVLEVNLRKLIQLKPDYAHAYNALGYTLADRTDRLAEA
                     VQFIEQALQLAPEDPFIIDSMGWVQYRLGNLKEGVTYLRRAFADRPDPEIAAHLGEVL
                     WVQGKHEEAETIWQTSLKDNPGNEALQNVIKKFGNK"
BASE COUNT         1213 a         1948 c         1699 g         1452 t
ORIGIN      
        1 aaatcaaacg cggcgcgaat caagcgattc aatcctggtt cctgcatgcc catgtccgcg
       61 agaaaaactt tcttgtcctc gtcgctgaga tcggcgatct ccgcttccag ctccgcgcaa
      121 atcgccagca ccggggcatt ctccgatttg gcatgtgccc gcactttgtc cagcagcgga
      181 ttgttctcaa acccatgctc cgcaacattg gccacataca tcgtgggttt cgcggtcaac
      241 aagaacagcg gctgtaacac cgcctgctcc tcccttgcga ggggcacact gcgcgcgagc
      301 ctggcttgat ccagcgcggg acgaatcttg tcgagcacgg cgatgacacg ctgcgcctct
      361 ttgtcggcgc ccgccttcgc cgccttggcg tagcgcgcat attgctttcc caccgttgcc
      421 agatcggcga gcgcgagctc ggtgttgacg acttcgatgt ctgaaacagg atcgatcctg
      481 ccggctacgt gcaccacgtt ctctttctcg aagcagcgca ctacgtgcac gatgccgtcg
      541 gtctcgcgaa tgtgacctag aaactggttg cccagtcctt cgcccttgga cgcacccgca
      601 accagccccg caatgtcaac aaactcggtg agcgcgggct gcactttttg cggcttggcg
      661 atgtcggctg tcgctttgag ccgagggtcg ggcacttcca cgacgccaac attgggatcg
      721 acggtgcaga atggatagtt ttccgcggcg acgccggcct tggtcagcgc attgaacagc
      781 gttgacttgc cgacgttagg caatcccaca atcccgcatc tgaggctcat ttatcctgct
      841 ttgtgtgcag tttgtgcatc gccgcctgaa gcttgccttc agcaatcagc ggccagactt
      901 cgaggctttg gtgaatggcc tgctcgatga gctttttttc ttctgcacgc gggtgatcga
      961 gcacgtaatc aacaaccagt gatttttctc ccggatggcc tatgccgata cgcagccgcc
     1021 agaaatcggg agagcccaaa tgcgcggcga tgtccttgag gccgttgtgg ccggaaacac
     1081 cgccgccctg cttcaggcgc gccacacccg gcggcaaatc gagttcatca tgtaccacca
     1141 gaatttcttg cgcggggatt ttgtaaaact ttgccagcgc gccgactgcg cggccgctgg
     1201 cattcatgaa ggtttgcggc tgcatcagcc agcattcttc gctatcgcgc gcaagtttgc
     1261 ccatcaaggc atggaaacgg gcctcaagct tgagcaaaac gcgtttctct ttggcaaggt
     1321 gtgcaagcca gcggtatccg gcgttatgcc ggtcagccgc gtgttccttg ccgggattgc
     1381 ctaatccaac gacgagtctc acattatgta taccgcataa acaaagcccg ccaattcagg
     1441 ggttccttaa tatggcgggc tttgcaaaat atcgcttaaa cgacctattt cttttctgct
     1501 ttctcgtctt tgccgccctc ctttttgccg gcttcaggct tgccaccttc tttcttgccg
     1561 ggttcaagct tgtcagccgc tttctcgcct tctttcttct cgccggtgac cgcacccgct
     1621 tcagccgcag cgccctcggc agccgccgct tcttcagcca ctggcgcagc taccggttcc
     1681 tccacgatta taatcttcgg cacttgcacg gttgccacta ccgcgtcgtt gccccttacc
     1741 agcgctgtgg actccgttcc tttggggaat ttgagctcgg acaggtgtac cgaatggccg
     1801 agcgccaaat ccttgagatc aacctcgata aattcgggca aatcggcagg caggcaggag
     1861 acttctactt cggccatgac gtgactgatg atgccgccgc caacttttac tcccggcgcg
     1921 atgtcggcat tgatgaagtg cagcggcact ttgatgtgaa ttttcttgtc cttggcgaca
     1981 cgctggaaat ccacgtgcat cacctgcatc ttgaagggat gcatgtgcac ttcgcgcagc
     2041 aatacctgca gttttttgcc gtccaggttc atgtcgagaa tcgaggcgtg gaacgtttcc
     2101 agtttgagca ggtggtacag cgcgttatgg tccagctcga tggtctgcgc ctcctgtttt
     2161 ccgccataga ggattccagg cacgcgtccg acacggcgca ggcggcggct cgcattcgtt
     2221 ccctgcagtg tgcgcgatat tgcggtgact tcgatttgtt gcattttgtt ctccggttga
     2281 ctagcgcagg ccggtcgcgg cttgcgcttt tttaaagcct tttactccat aaacaacgag
     2341 ctcaccgaat cctcgttgct gattcttagc atcgtctcgc ccaagagttc cgccacgcac
     2401 aagcagcgaa ttcgtttaca tttcgttgcg gcttcgctca gcgggatagt gttggtcacc
     2461 accagctcat ccagcaccga attctcgatg cgctctactg ctttacccga taacacgggg
     2521 tgcgtgcaat acgccagcac tttttccgcg ccttcgcgtt tcagcgcctc tgccgcctcg
     2581 cacaacgtat ttgcggtatc caccatgtcg tccatgatga tacaggtgcg gcctttgact
     2641 tcgccgatga tgttcatcac cttggctacg ttgggcctgg gacggcgctt gtcgatgatt
     2701 gccaaatccg cttccagggg cgtggcggcg gcgcgcgccc gcaccacccc gccgacatcg
     2761 ggcgaaacca ccaccaggtt ctggtgatcg tgcttccaga tgtcgcccag caaaatcggc
     2821 atggcgtaaa tattgtcgac cgggatgtcg aaaaaaccct ggatttgttc ggagtgtaaa
     2881 tccataatca ataaccggtc cacgcccacg ctggtcagca tgttcgccac catcttggcg
     2941 gtgattggca cccgcgccga acgcggcctg cggtcctggc gcgcgtaacc gaaatacgga
     3001 atcgccgccg tgatgcgccc ggcggaagcg cgtttcagcg catccaccat caccagcaat
     3061 tcaatcaggc tttcattggg cggcgagcaa gtggactgca gcacgaaaac gtccttgccg
     3121 cgtacgtttt ccagaatttc caccattact tcgccatcgc tgaaacggcc gacggtggcc
     3181 cgtccgaggt gaatgttaag atgtttcacc acctgctcgg cgagcttggg gttggcattg
     3241 ccggtgaaca ccatcagact gtcataagcc acgccgcttc ccccgatcgc ttgaggccgc
     3301 ttcacctaaa aaactctaaa cctggctggg gaggtaggga tcgaacctac gaatgccgga
     3361 atcaaaatcc ggtgccttac cacttggcga ctccccaata ttacagtgta agatccagca
     3421 aggggtgccg gtccaggccc cgcgccacaa aaccgctcat ctgccccggt aactgcgcca
     3481 gcactacgcg tgcttcccct tccgtcgcat actccgcaaa aacgcaggct ccggagccgg
     3541 tcattctggc cgcaccgaac tgtttcagcc acgccagatg ttgcgccacc tgcgggtatt
     3601 tgcggcacac tacaggctcc aggtcgttat gacctagccc tgtagaaaag gcggttattt
     3661 tgatgcgatt cgtgttccgt gtcaattcag gatcagcgaa aatctccgca gtggagacgt
     3721 tgaccttcgg caccaaaacc acataccagg ctggcggcaa gctgatgggt tgcagcactt
     3781 ctcccacccc ttcggcaaac gcgctttgac cgaacacaaa taccggaaca tccgcgccga
     3841 gcttgagtcc cagctgttgc agcctggcgc agctgaaatt caatttccat aagcggttga
     3901 gcgccatgag cgtggtcgcg gcatctgaac tgccgccgcc caatcctccg cccagcggca
     3961 gtctcttctg cagcgtgata tccacgccga gccgggtgcc cgcagtccgc tgcagcaact
     4021 ttgcggcttg cgcgcacaga tcgtctccct ccggcacgcc cggcagggcg taaccgcgct
     4081 tgataacacc atcatcacgc acgcggaagc tcaggctgtc ggcataatcc aaaaagcgga
     4141 acacgctttg cagcagatga taaccgtcct ccctgcgccc gacgacatgc agaaacaaat
     4201 tcagtttggc gggcgccgga taggtttgca gatcgctcat tgggcgaggg gggaaattat
     4261 ccagtcgtca attatcaggt ttatttgcag accgccgcgg ctcaatatga tgcgcgcggg
     4321 cataccgctt tcttgttcgg gcttgtagcg caggtaagcg atttcccacg agtcctggtt
     4381 taagcgcgcc aggcgctgct gcgcgtcgtg ctgcgcctgt gcggcgcttt gcggagcggc
     4441 aattcccaat atccaatact gcaatccgga gagcggcagg ctccacccca gcactgcgcg
     4501 cgtcaggctc tctgcatcga acgcgcggta ggttttctgc tcgttggtgg taaggctgat
     4561 tccgttggcg ttctggacga tgtgcgcaac accgctcccg agcggagcga gtatccagat
     4621 ttcatcgctg ctgccgttat gctcccagcg tacgcttcct gaaaaatttt tctcatcgta
     4681 tttgacggca acccgtccgg agagctggaa accgttgcgc tgggcggcca ccaggctctg
     4741 cgcccgtgac agcgccggcg agacgggttt aggcgggtga gcggcgcacg cgccaagcag
     4801 caacagaagc gcaagccaat aaacacggga acctctaacg aggttcctta tcacttgttg
     4861 ccgaatttct tgatgacatt ctgcaacgct tcgttgccgg gattgtcttt cagcgaagtc
     4921 tgccagatcg tttcggcttc ctcgtgcttg ccctgcaccc acaacacttc gccgaggtgc
     4981 gcggcaattt ccggatcggg gcggtcggca aacgcgcgcc gtaaataagt caccccttcc
     5041 ttgagattcc ccagccggta ttgcacccaa cccatgctgt cgatgatgaa cgggtcttcc
     5101 ggggcaagct gcagcgcttg ctcgatgaat tgcacggctt cagccagacg gtcggtgcgg
     5161 tccgccaggg tatagcccaa ggcgttgtaa gcgtgcgcgt aatcaggctt gagctggatg
     5221 agtttgcgca aattaacctc gagcacgtcc aagcggttga gtttttccgc cgccatcgcg
     5281 tagtcgtaca gcaagtccgg gtaatcggga tttttttcca gcgcgcgtcc cagcaaatca
     5341 aacgcttccc ggtagtgcgc cgcttcccgc agcaattgcg cttcggtgag aaggatttgt
     5401 atcctttgct gattatcctg caccgctatc tggccgagat acttgcgcgc ctcggcaagc
     5461 ttgccttgct tcgccagcac gccggcatag cgggtccgcg cgtttaaata gtgttcgccc
     5521 tcgcccacgc tgccgtacca gcgcaatgcc tcgtcgtaac gcctgcgctc ctcgtagatc
     5581 tgtcccaggt aaaagcgcac gctgtcgcgg tctttgtaat tctggctcag cgcgtgcttg
     5641 aagttttctt ccgccgcgtc gaaatcattg agttgcatcg acagcaaacc caccgccagc
     5701 gacacttccg cattattcgg gaaagccgcg cgcagcttct gaaattcgct gcgcgcctgg
     5761 gggtagtctt tttcagccac cagcaatctc gcataattca ggcgcacatc tttggccttg
     5821 ggatagctct tgaggtagcc gcgcaaatac tcgagggcga gcttgttgga ggtatgctgc
     5881 aaagtctgcc cttgaaagag cgccgcaagc tcccattccg gacgcaactc cagaaccttg
     5941 cgcacttcgc tcagcgcaac atcatattgc atcgcgttcc acgccgcctg cgctatcgct
     6001 aaatgcgctt ctgcaacgcg cggataaggt tgcgccagtt cctgcaccag attcagcgct
     6061 gcctgcttgt cgggatactt ggacagcagc ccgttcaatt gcatgaaacc ctggccgaca
     6121 ctttttccct ctgcggagag caacttttca atattcgggc gggcttcatc cagtttgcgg
     6181 ccgttaatca gcaaagccac cagtgtctgc cgtgccgggg tggactcggg atctacttcc
     6241 acccatattc ttgccgcctg cagcgccgcc tcgggctgcc ttgaaaacaa ggctatttca
     6301 gtggcgcgct gc
//
LOCUS       DATIOW010000043         9807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1338908, whole genome shotgun sequence.
ACCESSION   DATIOW010000043 DATIOW010000000
VERSION     DATIOW010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9807
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338908"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1395)
                     /locus_tag="VLL03_01305"
     CDS             complement(52..1395)
                     /locus_tag="VLL03_01305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSS46038.1"
                     /translation="MSNDSKLERSICDVLVIGGGPAGSTAAALLAEKGWQVVVLEKDR
                     HPRFHIGESLLPANVPLFERLGVKEVIERIGLLKYGAQFVSSYHGKSETFYFTEAVDK
                     SFPFSYQVRRSEFDHTLLKNCAAKGAAVYEQTRVSYIQFRRKQNSLITAVDANGVLHH
                     WETRFVIDASGRDTFLADFFGIKQRNRKHNSAALFAHFEGAERLPGKDAGNISVFWFD
                     HGWFWMIPLKDGAMSVGAVCWPYYLKSRKTDVEAFFLDTIAVCPGVAERMKHARLISP
                     VTATGNYSYQARYTAGEGFLMVGDAFAFVDPVFSTGVMLAMNSAFLGAEVVHDCLANR
                     RVSARRLREYDRRVRHGLKMFSWFIYRVTSPAMRYMFMNPANIFGTKEGLISLLAGDI
                     FGRTAVYRKILMFKLFYYLFSIRMWRECYAAYKVRKGNINPAALSPNESPARETA"
     gene            1625..2362
                     /locus_tag="VLL03_01310"
     CDS             1625..2362
                     /locus_tag="VLL03_01310"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HSS46039.1"
                     /translation="MRFLITFDDGPSIRTDYNPTLAILEQLARNDVQNGIKGIFFVQT
                     RNRNGGGTEAGKDIMRFTYAQGHVLGLHSAEPAGHVKHVRMGNEQLNQSLSNGIDDIR
                     HITGSNPEFVRPPGFLFDAETQELYRSLGLKMLLSDVNARDGVIHVFNVSLRRRSHIR
                     SELNRVYEAVVLRRLPVVHGVVPVMVTFHDVNTYTAWHFTEYLHILVEQAVNVGLPLA
                     AKPFFDQTGEIVEAARYRAVPPLITAK"
     gene            2471..2611
                     /locus_tag="VLL03_01315"
     CDS             2471..2611
                     /locus_tag="VLL03_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46040.1"
                     /translation="MLSIATVAPLFSLRWLFGCLIREFVLELRKEPNYSNLVISFRAR
                     RN"
     gene            2661..3575
                     /locus_tag="VLL03_01320"
     CDS             2661..3575
                     /locus_tag="VLL03_01320"
                     /inference="COORDINATES: protein motif:HMM:NF019127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HSS46041.1"
                     /translation="MRNWIVMGVLLAAMQFLSPGLLAAGLPPDYRQVLAYLDKKGDYK
                     NHVFKVGIPRNDLQVNVDGVDTPTAFGFGGWLAMTKGKNGLDVMMGDLVLLQDEVNPV
                     MSALLDNGLEVTALHNHFFWEEPRIFYMHVHGRGQAMELAKKVKPALDLIGNDAVGAS
                     QSEKSVDFAKGSLDAAALSRIIGHAGEQNGQVYKITLGRDDLKVDEMGARINSRMGLN
                     TWAAFYGSDERAVIAGDVAVLENEVTPVLRALRAHELDVVAIHHHMTGSRPMVIFLHY
                     WGQGQAVKLAEGFKSALDQLGLGTSRVH"
     gene            3603..3704
                     /locus_tag="VLL03_01325"
                     /pseudo
     CDS             3603..3704
                     /locus_tag="VLL03_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048529.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
     gene            3751..5139
                     /gene="mpl"
                     /locus_tag="VLL03_01330"
     CDS             3751..5139
                     /gene="mpl"
                     /locus_tag="VLL03_01330"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HSS46042.1"
                     /translation="MNIQILGICGTFMGGLAMLAKQAGHEVTGCDANVYPPMSTQLQA
                     QGIALTEGYAVGQISLKPDVFVIGNVITRGNPLMEEILNRGLPYISGPQWLAENVLRG
                     KWVLAVAGTHGKTTTASMLAWILEDAGLDPGFLIGGVPENFGISARLAPSSQPSPARG
                     EGAIVEPPFFVIEADEYDTAFFDKRSKFVHYRSCTTVLNNLEFDHADIFPDLTAIETQ
                     FHHLVRMLPANGLIVANGRDQNIKRVLSRGCWTPVEYFGIDQGWQAEGADESFAVCEK
                     GRTVGTLKWRLLGEHNRLNALAALLAARHAGVKVATGIAALEKFHNVKRRMEVKGVVN
                     GITVYDDFAHHPTAIRTTLEGLREKVNGARIIAVLEPRSNTMRMGLMKEELAASFAAA
                     DRVFCYTANLGWDAAAVLKPLGTRAQTCNDLQKLLTSIAQTVRPGDHVLIMSNGSFGG
                     IHDKLLKTLAQR"
     gene            5136..5705
                     /locus_tag="VLL03_01335"
     CDS             5136..5705
                     /locus_tag="VLL03_01335"
                     /inference="COORDINATES: protein motif:HMM:NF017538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HSS46043.1"
                     /translation="MTLIYIHGFNSSSASVKARLLREYLQDRGYADQFLAPDLSHWPS
                     RALAKLESEIRRRDPKTITLIGSSLGGHYATWLAEKHALRAVVVNPAVNPHLLLAPAL
                     GPQKNLYTGGEYQFTLQHLRELEQSNIAAITRPERYLLLVQTGDEMLDYHHAVEKYRG
                     AKQVVIEGGDHGFQNFSDHILLILEFAGK"
     gene            5776..7623
                     /locus_tag="VLL03_01340"
     CDS             5776..7623
                     /locus_tag="VLL03_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HSS46044.1"
                     /translation="MNVFYEEDGAFRVGAILADNGTTLQVEAAHGKRRKIKAAALLFR
                     FEQPGLNGFMERAQQAADDIDVDFLWSCCGEAEFGYDTLAREYFGRPPGPIESAALLI
                     RLHGSPMYFYKKGRGRYKAAAPDALKAALASIEKKRKQAAQQAAYVEQLTAFKLPAEF
                     GPMLPKLLYHPDRASIEYKALEAASASAHLTHAQLIEKCGAIPSSHDYHFQRFLLEHF
                     PEGAGFGDIGDIIEPQALPLAEVAAFSIDDAATTEIDDAFSVKRLKNGNWRIGVHIAA
                     PALGLAPGSKLDAIAARRLSTVYMPEGKITMLPEAVIERFTLAGGRSCPALSLYLDVL
                     PEDFTAVSSETRVERVPIAANLRHAELEQKFNEAALAAGKTGFPFGSELKLLWQFAES
                     LQKSRGQNETAPPPTDYNFTIEDGRVIITERKRGTPIDKLVSELMIYVNSAWGKLLAE
                     KGVAAIYRTQYNGKVKMSTAPGGHQGLGVEQYLWASSPLRRYVDLINQRQLVALVKGE
                     PPPCARNSETLLIAMRDFELAYEAYANFQRIMERYWCLRWIIQEAASLTPAAVVRENL
                     VKIDRLPLFCRVPSLPELPAGSRVEVQISAIDFLELTFSCEYRRKFPMQ"
     gene            8128..9051
                     /locus_tag="VLL03_01345"
     CDS             8128..9051
                     /locus_tag="VLL03_01345"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HSS46045.1"
                     /translation="MRFDITSLKYTAVSLGNKITLMDVATRLQVAMFLSFTLHSILLL
                     GVTFTFPDAPKRSHDGLPLEVVLVNSKTVSKPLQPDALAQANLDGGGNVDQRRRAKSP
                     LPVLKKKLEVPDQLAQAQRRVKKLEAQSQKLLTQAQSAKKLEQMESKPQPERQKVESP
                     SAMDLMQKSLEAVRLEAEIAREREEYQQRPRRMFIGARTREYAFTRYVDDWRIKVERV
                     GNLNYPEAARRQKIYGSLVLTVSINADGTVENVEINRSSGNKILDAAAIRIVELSSPF
                     APFPEEMRKKVDILSITRTWTFTRSDQLVGE"
     gene            9058..>9807
                     /gene="aroE"
                     /locus_tag="VLL03_01350"
     CDS             9058..>9807
                     /gene="aroE"
                     /locus_tag="VLL03_01350"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HSS46046.1"
                     /translation="MADRYAVIGNPVSHSKSPAIHAEFARQTGQNISYLPILAPLHGF
                     VAAVKDFINQGGKGMNVTLPFKEEAFRLAMPNVSIRAREAEAVNTMRFDGGLIVGENT
                     DGMGLVKDIQHNLMQSALTGKRILLLGAGGAARGVILPLLEFKPALLTVANRTFSKAS
                     ALEQRFTRYGNIKASEYAALAGNKYDLVINATSASIAGELPPLPAKLFTSDSLAYDLM
                     YGGGLTPFLRFAQAQGAARLADGLGMLVEQAA"
BASE COUNT         2140 a         2740 c         2860 g         2067 t
ORIGIN      
        1 ctgccgacag cggagctttt tgcgtgggta gcgcgcgcgc ctgccgttga ttcatgcggt
       61 ttcccttgcc ggggattcgt tcgggctcaa ggcggcaggg ttgatatttc ctttgcgcac
      121 tttataggcc gcgtagcact ccctccacat gcggatgctg aagagataat aaaacagctt
      181 gaacatcaaa attttccggt aaactgccgt gcgtccgaaa atatctcccg caagcagcga
      241 aatgaggccc tctttcgtgc cgaaaatgtt tgccgggttc atgaacatat agcgcatcgc
      301 cggtgaggtg acgcggtaga taaaccagga aaacatcttc aggccgtgcc gtacgcggcg
      361 gtcgtattcc ctcaaccttc gcgcggaaac gcggcggttg gcaaggcaat catgcacaac
      421 ttcggcccca agaaacgcac tgttcatcgc cagcatcacg ccggtggaga ataccggatc
      481 gacgaaagcg aacgcatcgc cgaccatgag aaacccttca cccgccgtgt agcgggcctg
      541 ataggaataa ttgccggttg ccgttaccgg cgaaataagc ctggcgtgct tcattcgctc
      601 ggcgacgccg gggcatactg cgatggtgtc caggaaaaaa gcttcgacat cggtttttcg
      661 ggattttagg tagtaaggcc aacataccgc gcccacgctc atggcgccgt ccttcaacgg
      721 gatcatccag aaccagccgt gatcgaacca gaacacgctg atgttgccgg catcctttcc
      781 cggaaggcgt tcggcgccct cgaagtgcgc gaatagcgcg gcgctgttat gtttccggtt
      841 gcgttgcttg attccaaaaa aatcagcgag aaaagtgtcc ctgcccgacg cgtcgatgac
      901 gaatcgtgtt tcccaatggt gcaaaacccc gtttgcatcc actgcggtga tgagggaatt
      961 ttgctttctg cgaaattgaa tataacttac tcgtgtctgt tcatatactg ccgcgccttt
     1021 ggccgcgcag tttttcagca gggtatggtc aaactcggag cggcgcacct ggtaggaaaa
     1081 aggaaaactt ttatccactg cttcggtgaa ataaaaggtt tcactcttgc cgtggtaact
     1141 ggagacgaat tgcgccccgt atttgagcag gccgattctc tctattacct ccttgacacc
     1201 cagccgctca aacaggggaa cattggcggg aagcagcgat tcaccgatgt ggaaacgcgg
     1261 atgccggtct ttttccagca ccaccacttg ccagcctttt tctgcgagca aggcggcggc
     1321 ggtcgagccg gcgggcccgc cgccgattac cagaacatca caaatgcttc tttctaactt
     1381 gctgtcattg gacataaaat gtctgcctcg ttcctgatgc cggccggcgc gggtaggcgg
     1441 agtgaaggga tttctcgttt gaaatcacga tcataagttg aattatacga aaataatggt
     1501 tgtgctgcat aaacctacaa tcaaaaattt tgcaatctcg ggcatacggc ttttgctgct
     1561 ggccggtctg ctgctgagtg gcggctgtca gtcgttgcac cctgcagcgt cccccgcggt
     1621 aaaagtgcgt tttctgatca cattcgacga tggcccgagc atacgcactg attataatcc
     1681 cacgcttgcg attcttgagc agttggcgcg caacgacgtg caaaacggca tcaaggggat
     1741 tttcttcgtg cagacgcgca accgtaacgg agggggcacc gaggccggca aagacatcat
     1801 gcgctttacc tacgcacagg gccacgtgtt ggggctgcac agcgccgagc cggccgggca
     1861 tgtaaaacac gtgaggatgg gaaatgagca gctcaaccag tcgctcagca acggcataga
     1921 cgatatcagg catatcaccg gcagcaatcc ggaatttgtc cgtcccccgg gattcctgtt
     1981 tgatgccgaa actcaggaac tttaccgaag tctgggccta aagatgttgt tatcagacgt
     2041 caacgcaagg gacggggtca tccatgtttt taacgtcagt ttgcgccggc gcagtcatat
     2101 ccgttccgaa ctcaaccggg tttacgaggc agtagtcctg cgccggttgc ctgtggtgca
     2161 tggcgtggtt ccagtgatgg tgacatttca cgacgtgaac acctataccg cctggcactt
     2221 taccgagtat ctgcacatac tggtggaaca agcggtcaac gtcggcttac ccctggctgc
     2281 gaagcctttt tttgaccaga ccggagaaat tgtggaggcc gcgcggtacc gggctgtgcc
     2341 accgcttatc accgccaagt gaaattcaaa accgcataca aaaaaataga gattggggtt
     2401 ggccttgctg catcctgcgt ttggctcgta ctgctgcttc tgccgcaggc ccggcgcccg
     2461 atgagaagtg atgctttcga ttgcgactgt ggcgccgctg ttttccctgc ggtggctgtt
     2521 cggctgcctc atcagggaat tcgtgctgga acttaggaaa gagcccaact actcgaatct
     2581 tgtcatcagc ttccgcgcgc gaaggaattg acgaattggc gaaaactcaa gttgaattaa
     2641 attcagtcgg ggagaaaatc atgcgaaatt ggatagtcat gggtgtcctg cttgccgcaa
     2701 tgcagttttt gtcgccgggt ttgttggcgg cggggttgcc gccggattat cgacaggtgc
     2761 ttgcctacct tgacaaaaag ggcgactaca agaatcacgt tttcaaggtg ggcattccgc
     2821 gcaacgatct gcaagtgaac gtggacggcg tagacacgcc gacggcgttc ggttttggcg
     2881 gctggctggc gatgacgaaa ggcaaaaacg gccttgacgt aatgatgggc gatcttgtgc
     2941 ttttgcagga cgaagtcaat ccggtgatgt cggccttgct ggataacggg ctcgaagtca
     3001 ccgcattgca taaccatttt ttctgggaag agccgcgcat tttctacatg cacgtgcatg
     3061 gccgtggcca agcgatggaa ctggcgaaaa aagtaaagcc tgcgctggat ttgattggca
     3121 acgacgcggt cggcgcttca cagtcggaaa aaagcgttga tttcgctaaa gggtctttgg
     3181 atgccgctgc gttgtcgcgc atcatcggcc atgccggtga gcaaaacggc caagtttata
     3241 aaatcaccct cggtcgcgac gacctgaagg tcgatgaaat gggcgcgcgc atcaattcgc
     3301 gcatgggact caacacctgg gcggcgtttt acggcagcga cgagcgggca gtgatcgcgg
     3361 gagatgtggc ggtgctggaa aacgaagtga cgccggtgct gcgcgcgctg cgcgcgcacg
     3421 agttggatgt agtggcgatc catcaccaca tgaccggctc gcggccgatg gtgatttttc
     3481 tgcattactg gggccagggg caggccgtga aacttgcgga aggatttaaa tcagcgcttg
     3541 atcagcttgg cctcggcacg agccgcgttc attaaattta tgcacgattt aaccgcaaag
     3601 agcaccgcgg ctgcaccacc caagtttgag acgccagggt ggaagacgcc gcttcgggca
     3661 agaccattgc gttgttccgc tgcacgcaaa tggtactgtg gtaaggggtc agcgactata
     3721 ctgcacctct gctgacggcg gcgtttttcc atgaacatcc aaattctcgg catctgcggt
     3781 accttcatgg gcggccttgc catgctcgct aagcaggcgg gacatgaagt caccggctgc
     3841 gacgccaatg tctacccgcc gatgagcacc cagcttcaag cccaaggcat tgcactcacc
     3901 gagggttatg ctgtcgggca aatcagcctt aagcccgatg tgttcgtgat cggcaatgtg
     3961 attacccgcg gcaatccgct gatggaggaa attctgaatc gcgggcttcc ctatatttcc
     4021 ggcccgcagt ggctggcgga gaatgtgctg cgcggcaaat gggtattggc ggtggccggc
     4081 acacacggga agaccaccac cgcttccatg ctggcctgga ttctggaaga tgccgggctt
     4141 gatccgggct ttctcatcgg cggcgtgccg gagaatttcg gcatttccgc gcggcttgcc
     4201 ccctcatccc aaccttcccc cgcaaggggg gaaggagcaa tcgttgaacc tccttttttt
     4261 gtgattgaag cggacgaata cgacaccgct ttcttcgaca agcgttcgaa attcgtgcat
     4321 taccgttcat gcaccacggt gctgaacaat ctggaattcg accacgccga tatcttccct
     4381 gatcttaccg ccatcgaaac ccagttccac catctggtgc gcatgcttcc cgcgaacggc
     4441 ttgattgtcg ccaacggccg cgaccaaaac atcaagcgcg tgctttcgcg cggctgctgg
     4501 acgccggtgg aatactttgg catagaccag ggctggcagg cagaaggcgc tgacgaaagc
     4561 tttgccgtgt gcgaaaaagg caggacggtg ggcacgctga agtggcggct tctgggcgaa
     4621 cacaaccgtc tcaacgctct cgcggcgctg ctcgccgcgc gtcacgccgg ggtgaaggtg
     4681 gccaccggca ttgcggccct ggaaaaattc cacaacgtga agcggcgcat ggaggtaaaa
     4741 ggggtggtga acggcattac cgtttacgat gattttgccc atcatcccac cgcgattcgt
     4801 accacgctcg aaggcctgcg cgaaaaagta aacggcgcgc gcattattgc ggtgctggaa
     4861 ccgcgctcca ataccatgag aatggggctg atgaaagaag agctcgcggc aagcttcgcc
     4921 gccgcggacc gggtgttctg ctacacggcg aatctcggct gggatgccgc cgccgtcctc
     4981 aaaccgctgg gaacgcgggc tcaaacctgt aacgacttgc aaaagctgtt gacctcgatc
     5041 gcgcagactg ttcgccccgg tgatcatgtg cttatcatga gcaacggcag tttcggcggc
     5101 attcacgaca agctgttaaa gacacttgcc caacgttgac gctgatttat atccacggct
     5161 tcaacagctc atcggcctcg gtgaaggcgc ggctgctgcg cgaatatctg caagaccgcg
     5221 gatatgccga tcaatttctg gcacccgatc tgtcgcattg gcccagccga gcactagcca
     5281 agctggaatc agaaatccgc cgccgtgatc ccaaaacaat taccctgata ggtagctcac
     5341 tgggcggaca ctatgcaacc tggcttgcgg aaaaacatgc tctgcgggca gtagtggtga
     5401 atcccgcggt gaacccgcac cttttgcttg cgcctgccct cgggccgcag aagaatcttt
     5461 acaccggcgg ggaatatcag ttcacgctac agcatctcag ggaacttgag caatccaaca
     5521 tcgccgccat tacccggccc gagcgctatc ttttgctggt gcagaccggc gacgagatgc
     5581 ttgattacca ccatgccgtg gagaaatacc ggggcgcaaa gcaggtggtg attgaaggcg
     5641 gcgaccacgg ttttcagaat ttttccgacc atattttgct gatcctggag tttgctggaa
     5701 aataattcct gcgctcctgg ggtataattt cagaattcca attcctgttt cgcggcgctt
     5761 gcctgcgcgg tttccatgaa cgttttttac gaagaagacg gcgctttcag ggtaggcgct
     5821 atccttgccg ataacggaac cacgctgcaa gtcgaggccg cgcacggcaa gcgccgcaaa
     5881 atcaaggcgg cggcgttgtt gttccgtttt gagcagcccg ggttgaacgg ctttatggaa
     5941 cgcgcgcagc aggcggcgga cgacatcgat gtggatttcc tgtggtcctg ctgcggcgag
     6001 gcggagtttg gctacgatac tttggcgcgc gaatacttcg ggcgcccacc cggtccaatc
     6061 gagtctgccg ccctgctcat ccgtttgcac ggctcgccca tgtattttta caagaaaggc
     6121 cgcggccgct acaaggcggc ggcgccggac gcgcttaaag cggccttggc cagcatcgag
     6181 aaaaaacgca agcaggccgc gcagcaggcc gcgtatgtgg agcagttaac ggcgttcaag
     6241 ctgccggcgg aattcggtcc aatgctcccc aagctgctct accacccgga tcgggccagc
     6301 atcgagtaca aggcgctgga agcggcgagc gcgtcggcac atcttactca tgcgcagctt
     6361 atcgaaaaat gcggcgccat tccatcatcg cacgattacc acttccagcg cttcctatta
     6421 gagcattttc ccgagggcgc cggttttggc gatatcggcg atatcatcga gccgcaggcg
     6481 ctgccgcttg ccgaagttgc agctttcagc atcgacgatg cggccaccac ggaaatcgac
     6541 gacgcgtttt cggtaaaacg cctcaaaaac ggcaattggc gcatcggcgt gcacatcgcg
     6601 gcgccggctt tggggttggc gccgggatca aaacttgatg caattgcggc gcgacggctt
     6661 tccacggtgt atatgccgga aggcaaaatt accatgctgc cggaagcagt gattgagcgt
     6721 tttaccctgg ccggagggcg gtcctgcccg gcgctgtcgc tttatctgga cgtgctaccg
     6781 gaggatttta ccgccgtttc atcggaaacc cgggttgagc gcgtgcccat cgcggcgaat
     6841 ttacggcacg cggaactgga acaaaaattc aatgaggccg cgcttgccgc aggcaagacc
     6901 ggttttccct tcgggagcga gctgaaactg ctgtggcagt ttgccgaaag cctgcaaaaa
     6961 tcgcggggcc agaacgaaac ggcgccgccg cccactgatt acaacttcac cattgaggac
     7021 ggccgggtca taatcaccga gcgcaagcgc gggacaccaa tcgacaagct ggtctccgag
     7081 ctgatgattt atgtgaacag cgcctggggc aagttgctgg ccgaaaaggg cgtagccgcg
     7141 atttaccgca cacagtacaa cggcaaggtg aaaatgagca ccgcgccagg cgggcaccag
     7201 ggtttgggcg tggagcaata cctgtgggcg agttcgccgc tgcgacgcta tgtggacctt
     7261 atcaatcaac gccagctggt ggccttggtc aagggagagc cgccgccttg tgccagaaac
     7321 agcgaaacac tgctgattgc catgcgcgat tttgagttgg cctatgaagc atatgccaat
     7381 ttccagcgca tcatggaacg ctattggtgc ctgcgctgga taatccagga agccgcgagc
     7441 ctcactccgg ctgcggtggt gagggaaaat ctggtgaaaa tcgaccggct gccgcttttt
     7501 tgccgcgtgc cttcgctgcc cgagctgcct gccggcagcc gggtcgaagt gcaaatttcg
     7561 gccatcgatt tcctggagct tactttttcg tgcgaatacc ggcgcaagtt cccgatgcag
     7621 tgacaggatg cgttttgttg cttacaatgc gacatctgca tctgtcgcct ggggtaagga
     7681 acctctgatt aagtcctaca tgaggcgcgt tgcgagcaaa aagcggatga acgcaaggcg
     7741 cgcgacgcag gtcgtagtta agcctacgaa gccaagaagg gcccgcgtag cgtcgcacct
     7801 gcaaagctgt gcagtgaagc gggccgaccg ctcgcctctg cggtcgcacc tgccgaggct
     7861 ccacgcttcg gcgagcacgc cgcagacgcc gcaagccctc atcctagcct tctcccagag
     7921 ggagaaggaa cgaacgcccc ctctcccgca tgcgggagag ggatggggtg agggtacggc
     7981 cgcaacccgg agggacggag cgttaagagc atcatgcttt gtcgctcgtc gcttatttgg
     8041 catccaccaa atggcgctcc ttgcttcacg cctgtcgccc ttaacccaac gtcgcgccca
     8101 tgctggactc aatcagaggt tccctaggtg cgtttcgata tcacatcgct gaaatacacg
     8161 gccgtatcac taggcaataa gattactctg atggacgttg ccacgcgcct gcaagtggca
     8221 atgtttctct ccttcaccct gcattcgatt ttactgctcg gcgtcacctt cacgtttccc
     8281 gatgcgccca agcgcagcca tgacggcctg ccgctggaag tagtgctggt gaacagcaag
     8341 accgtttcca agccgctgca gccggacgcg ctggcgcagg ccaaccttga tggtggcggc
     8401 aatgttgatc agcggcgccg cgccaaaagc ccgctgccgg tgttgaagaa aaagctggag
     8461 gtccccgacc agctcgcgca agcgcagcgc cgggtgaaaa agcttgaagc gcagtcgcag
     8521 aagctgttga cgcaagccca atccgccaaa aaactcgagc agatggaaag caagccccag
     8581 cccgagcggc agaaggtgga aagccccagc gccatggatc tgatgcaaaa gagcctggaa
     8641 gcggtgcggc tggaagcgga aattgccaga gagcgcgagg aataccagca gcggccgcgg
     8701 cgcatgttca tcggcgcgcg cacccgggag tacgcgttta cccgctacgt ggacgactgg
     8761 cgcatcaagg tagagcgcgt cggcaatctc aattacccgg aagcagcgag gcgccagaag
     8821 atttacggca gcctcgtgct caccgtatcc atcaatgccg acggcacggt ggaaaacgtg
     8881 gaaatcaacc gctcttccgg caataaaatc ctggatgccg cggccatccg catcgtcgag
     8941 ctgtcgagtc cgttcgcgcc gtttcccgag gaaatgcgca agaaagtgga tatcttgagc
     9001 atcacccgca cctggacctt tactcgcagc gatcagttgg tgggggagtg attccctatg
     9061 gctgaccgct acgccgtcat cggtaacccc gtttcccaca gcaaatcgcc cgcgatacat
     9121 gccgagttcg cgcggcaaac cgggcagaac atatcttacc tgccgatcct cgcaccactc
     9181 catggatttg tcgcggcggt gaaagatttc ataaatcagg ggggcaaagg catgaatgtc
     9241 actttgccat ttaaagagga ggctttccgg cttgctatgc caaatgtttc gattcgggca
     9301 agagaagccg aggcggtgaa cacgatgcgt tttgacggcg ggttgattgt cggtgaaaac
     9361 acggacggaa tgggattagt taaggatatt cagcataacc ttatgcaatc ggctttgacc
     9421 gggaagcgca tcctattatt gggagcgggc ggcgccgctc gcggcgtgat tctgccttta
     9481 ttggagttta agcccgcctt gttgacagtc gccaaccgca cgtttagcaa agcgtcggcg
     9541 ctcgaacagc gttttacccg ttacggcaac attaaagcca gcgaatacgc ggcccttgcg
     9601 ggtaataaat acgacttggt tataaacgct acttccgcca gtattgccgg cgaattgccg
     9661 ccgttgcccg caaaactttt cacatccgac tcgctcgcct acgacctgat gtatggcggg
     9721 gggctcactc cttttctgcg gtttgcacag gcacaaggcg ccgccaggct tgccgacggc
     9781 ttaggcatgc tggtggagca ggcagcg
//
LOCUS       DATIOW010000044         5371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1541330, whole genome shotgun sequence.
ACCESSION   DATIOW010000044 DATIOW010000000
VERSION     DATIOW010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541330"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /gene="glcF"
                     /locus_tag="VLL03_01355"
     CDS             <1..925
                     /gene="glcF"
                     /locus_tag="VLL03_01355"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HSS46047.1"
                     /translation="KRVARGFIDGAMRALMRTVLPNPALFNPLLKLGQLARPVLPEKI
                     RRKIPRAAQPTAWPAARRARRMLLLEGCVQPALAPNINPATARVLDRLGISLARASGC
                     CGAITYHLNYQEQGLIIMRRNIDAWWPQIEQGAEALVINASGCGAMVKEYGNLLKDDA
                     AYAAKAQRISTLANDVSEIIVVNKDRIAAQTGKKGVNTRLAFHAPCTLQHALEIRGVV
                     EPLLADLGFELCDVPDAHLCCGSAGTYSLLQPELSRKLLANKIAALESGKPGEILTAN
                     IGCLAHLQSATDLPVRHWIEALDELLAVRSP"
     gene            complement(917..1846)
                     /locus_tag="VLL03_01360"
     CDS             complement(917..1846)
                     /locus_tag="VLL03_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46048.1"
                     /translation="MIKTFAFPALVALLLSFASGHAQVADISDTARLEVAGGTLDVIF
                     APGELDLPRSVVLDWISKSAHAVAGYYGRFPVREAQITIVPVASSGVQSGKSFGYPRA
                     RVRISLGRSATEADLEKDWMLVHELVHLAFPSVDNSHHWIEEGIATYVEPIARAEAGQ
                     LSVEKVWGDLVRGLPYGLPQTGDRGLDFTPTWGRTYWGGALFCLLADIEIRKRTQNRH
                     GLQDALRAIVAAGGSIEESWDLRRAFKIGDAATGVPVLTELYERMRSTPVDTPLSELW
                     RSLGVEVQGRSVTFNEGAPLAGVRRAITAGRSG"
     gene            complement(1943..3028)
                     /gene="queG"
                     /locus_tag="VLL03_01365"
     CDS             complement(1943..3028)
                     /gene="queG"
                     /locus_tag="VLL03_01365"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HSS46049.1"
                     /translation="MHDSTFSHSSTDEFAELAAAIKSWGKALGFQEVGIAGVDLGDAE
                     QRLLEWLAAGCHGDMDYMAKHGMKRSRPAELLPGTQRVISVRLNYQPQDARDSWEVLG
                     QGNKAYISRYAVGRDYHKVLRSRLQQLVDKIREYAGDYRCRVFTDSAPVMEVELARKA
                     GLGWRGKHTLLLTRETGSWFFLGEIYTDLPLPADAPQPDHCGTCQKCMDICPTQAITA
                     PYQLDARRCISYLTIEHQGSIPEALRPLMGNRVYGCDDCQLVCPWNKYAKPSGEPDFK
                     VRHGLDDAELVELFGWSEEEFHAKLAGSAIHRIGYERWLRNLAVGLGNAPASAAVIAA
                     LERRCDHPSPLVREHVAWALSNQRAAA"
     gene            3023..3514
                     /gene="tsaE"
                     /locus_tag="VLL03_01370"
     CDS             3023..3514
                     /gene="tsaE"
                     /locus_tag="VLL03_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HSS46050.1"
                     /translation="MHKPHHNQPDTKIIRHLADEQATRALGVQLAPLITPGLKVYFCG
                     ELGSGKTTLVRGLLEGLGYRGKVKSPTYLLVELYAISRLDLYHFDFYRFKLAEEWQDA
                     GFREHFDSQAVCLVEWPEKAAGLLPVPDIKISLQIESAGRAVTIEGVTETGRECLKLL
                     QDT"
     gene            3487..4839
                     /locus_tag="VLL03_01375"
     CDS             3487..4839
                     /locus_tag="VLL03_01375"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HSS46051.1"
                     /translation="MFKASTRYVTRYASRKAFSTIFTLFFSLAFVSGAYAAIEIGSAR
                     IWPSQDYTRLTLESKEPIAHKLFTIKNPERLVLDLENVELTTALENISAKIAPSDPYI
                     KQVRVGRFKPGVVRLVLDLKTEVKPQVFILKPVAEYGHRLVLDIYPAQPVDPLLALAL
                     KQENKLGSLNDNAASAQNADEPSVPAEPTAQAKNEEPRHAEGKQEKKPQPEVARLITI
                     AIDPGHGGEDPGARGRGGTREKYVTLAIARKVKAKVDEEPNMRGVLIRDSDYFIELQE
                     RVRKAQKIKADLFISIHADAYIKPHARGSSVFALSERGATSAAARWLANKENESDLIG
                     GVNLDVRDVYLKQTLLDLAQTATINDSVKLGKAVLSELGSVNNLHRHSVEQAGFAVLK
                     APDIPSILVETAFISNPEEEIRLKEESYQNKIADAIVGGIKRYFVKNPPLAKSKLARY
                     "
     gene            5091..5231
                     /locus_tag="VLL03_01380"
     CDS             5091..5231
                     /locus_tag="VLL03_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46052.1"
                     /translation="MLAKLNPVYAKIAVALIISVLAFRLGGLAGHTEYRLPAATQSEC
                     SR"
BASE COUNT         1182 a         1634 c         1518 g         1037 t
ORIGIN      
        1 aaagcgcgtg gcgcgcggtt tcatcgacgg cgcgatgcgc gccttgatgc ggaccgtgct
       61 gccgaacccg gcgctgttta atccgctatt gaaactgggc caactcgcgc gcccggtgct
      121 gcccgaaaaa atccgccgca aaattccccg agccgcccaa ccgacagcgt ggccggccgc
      181 acggcgcgcg cgcagaatgt tgctgcttga aggctgcgtg caacctgcgc tggcgcccaa
      241 tatcaatccc gcgactgccc gagtactcga ccggctcggc atctcgcttg cgcgcgcgtc
      301 aggctgctgc ggtgccatca cttaccatct caattatcag gaacaaggat tgataatcat
      361 gcgccgcaat attgacgcct ggtggccgca aatcgagcaa ggcgcggaag cgctggtaat
      421 caacgccagc ggctgcggcg cgatggtgaa agaatacggg aatctactta aagatgacgc
      481 cgcctacgcg gcaaaagcgc agcgcatttc caccctggcc aacgacgtga gcgaaatcat
      541 cgttgtcaac aaagacagaa tcgctgcgca gacgggaaaa aaaggcgtta acaccagact
      601 cgctttccat gcgccctgca ccctgcagca cgcgctggaa atccgcggcg tggtggaacc
      661 gctgcttgcg gacctgggtt tcgagctgtg cgacgtgccc gatgcgcatt tgtgctgcgg
      721 ctcggcggga acctattcgc tgttgcagcc tgagctgtcg cggaaactgc ttgccaataa
      781 aatcgccgcg ctggaatcag gcaagccggg agaaattctc accgcaaata tcggctgtct
      841 cgcgcatctg caaagcgcga ccgacttgcc ggtgaggcat tggattgaag cgctggacga
      901 attgctcgcg gttcgttcac cctgaacgac cggcggtaat cgcacggcgc acgccagcga
      961 gcggcgctcc ctcattgaaa gtcacgctgc ggccctgcac ttccacgccg aggctgcgcc
     1021 acagctcgga taacggcgtg tccaccggcg ttgagcgcat gcgctcatac agctcggtga
     1081 gtaccggcac gccagtcgcc gcatcgccga ttttaaaagc gcggcgcaag tcccagcttt
     1141 cttcgatgct gccgccggcg gcgactatcg cacgcaacgc gtcctgcagg ccgtggcggt
     1201 tttgcgtgcg cttgcggatt tcgatgtcgg caagcaggca gaacaacgcg ccgccccaat
     1261 aagtgcgccc ccaggtcggc gtgaagtcca gcccgcgatc ccccgtttgc ggcaggccgt
     1321 aaggcaaacc gcgcaccaaa tcgccccaga ctttttccac gctcaattgc cccgcttcgg
     1381 cgcgggcaat cggctcgaca taagtggcga ttccttcctc gatccaatga tgagaattat
     1441 caaccgacgg aaaagcaagg tgcaccagtt catgcaccaa catccagtct ttctccagat
     1501 ctgcttccgt tgcagagcgg ccgagagaga ttctcacccg cgcgcggggg tagccgaagg
     1561 atttgcctga ttgcacgccg ctgctagcga cgggaacaat ggtgatctgc gcctcgcgca
     1621 ccggaaatcg gccgtagtaa ccggcgacgg catgcgccga tttggaaatc cagtcgagca
     1681 ccacggatcg cggcagatcc agctcccccg gcgcaaaaat cacatccagc gtgccgccag
     1741 cgacctcgag cctggcagta tcggaaatgt ccgccacctg cgcatgccct gaagcaaagc
     1801 ttaacagcaa ggctaccagc gcgggaaaag caaatgtttt tatcactcga tggcctggct
     1861 caagggagat tttttattcc tggcagcaga ttcgactgcg gcaacgccca taaattcacc
     1921 ggcacgcggg cctcaatgat tttcatgctg ccgcgcgctg atttgaaagc gcccaagcca
     1981 catgctccct caccagcggc gaggggtgat cgcagcggcg ctcgagcgcg gcaattaccg
     2041 ctgctgaagc cggcgcgttg ccgagtccca cggcgagatt tctcagccag cgctcatagc
     2101 cgatgcgatg aatggcgcta cccgcgagtt tggcgtggaa ttcctcttcg ctccagccaa
     2161 acagctcaac caattcggcg tcatccaagc cgtggcgcac cttgaaatcc ggctcgcctg
     2221 aaggtttggc atacttgttc caggggcaga ccaattgaca gtcgtcgcag ccgtacacgc
     2281 gattccccat gagcgggcgc aatgcttcgg gaatgctgcc ttggtgctcg atggtgagat
     2341 aagatatgca gcgccgcgcg tcgagctgat acggcgcggt aatcgcctgc gtcgggcaaa
     2401 tgtccatgca tttttggcag gtaccgcaat gatccggttg cggcgcatcc gccggcagcg
     2461 gcagatcggt gtaaatctcg ccgagaaaaa accacgagcc ggtctcacgc gtgagcagca
     2521 gcgtatgctt gccgcgccag cccaaacccg ctttgcgcgc gagttccacc tccatcaccg
     2581 gcgcgctgtc ggtaaacacg cggcagcggt aatcgcccgc gtattcgcga atcttgtcca
     2641 ccagctgctg caagcgcgag cgcagtactt tatggtaatc gcgccccacg gcgtagcggg
     2701 agatgtaggc tttgttgccc tgcccgagca cctcccagct gtcgcgcgcg tcttgcggct
     2761 ggtaattcaa gcgcacggaa atcacgcgct gcgtgccggg caaaagctcc gccgggcggc
     2821 tgcgtttcat cccgtgtttt gccatataat ccatgtcgcc gtggcagccc gctgccagcc
     2881 attccaagag ccgctgttcc gcgtcgccaa gatctactcc ggcgatgccg acttcctgga
     2941 agccgagcgc ttttccccat gacttgatcg ccgcggcgag ctcggcaaac tcatccgtcg
     3001 aactatgact gaaagtggaa tcgtgcataa acctcatcat aaccagccgg acacaaaaat
     3061 catacgccac ctcgccgacg agcaggccac ccgcgcactc ggcgtgcagc ttgcgccgct
     3121 gataacgccg ggattgaaag tttatttctg cggcgagctc ggctcgggca aaaccacgct
     3181 ggtgcggggg ctgctggaag gcctgggcta ccgcggcaag gtcaaaagcc cgacgtattt
     3241 actggttgaa ctttatgcta tttctaggtt agacttatat cactttgatt tttatcggtt
     3301 caaacttgcg gaagaatggc aggatgcagg attccgcgaa cacttcgaca gccaggccgt
     3361 ctgtcttgtc gaatggccgg aaaaagccgc gggcctgctt cccgtccccg atatcaaaat
     3421 cagcttacag atagaaagcg caggccgcgc tgtcacgatt gaaggagtca ccgagaccgg
     3481 cagagaatgt ttaaagcttc tacaagatac gtaacccgat acgcctcccg caaggccttc
     3541 tccacaattt ttactctgtt tttttccctg gctttcgttt ccggcgcgta cgcggcaata
     3601 gaaatcggct ccgcgcgcat atggccgtcg caggattaca cgcggctcac cctcgaatcg
     3661 aaagagccga tcgcgcataa acttttcacc atcaagaacc ccgagcggct ggtgctcgat
     3721 ctggaaaacg tcgagctcac taccgcgctg gaaaatattt ccgccaagat tgcgccttcc
     3781 gatccttaca tcaagcaggt gcgcgtaggg cgcttcaagc cgggcgtggt gcgtctcgtc
     3841 ctcgatctga aaaccgaagt caagccgcag gtgttcattc tgaaaccggt cgccgaatac
     3901 ggccaccggt tggtgctcga catttatccg gcacagccgg ttgatccgct gctggcgctg
     3961 gcgctgaagc aggagaacaa gctggggtca ctgaacgaca atgctgcgtc cgcgcaaaat
     4021 gcagatgagc cttccgtgcc ggccgagccc actgctcagg ccaagaacga agagccaagg
     4081 catgctgaag gcaaacagga gaaaaaaccg caaccggaag tggcacggct cattaccatc
     4141 gcgattgacc ccgggcacgg cggcgaagat ccgggggccc gcggccgcgg cggcacgcgc
     4201 gagaaatacg tgacgctggc tatcgcgcgc aaagtcaagg ctaaagtgga cgaggagccg
     4261 aatatgcgcg gagtgctgat ccgcgacagc gattacttta tcgagttgca ggaacgggtg
     4321 cggaaggcac aaaaaataaa agccgactta tttatttcga tccacgccga tgcctacatc
     4381 aagccacatg cgcgcggctc atcggtattt gcgctttccg agcgcggcgc aacctccgcc
     4441 gccgcacgct ggctcgccaa caaagaaaat gagtcggatt tgatcggcgg ggtcaatctc
     4501 gacgtccgcg acgtttatct taagcaaact ttactcgacc tggcacaaac cgccaccatc
     4561 aacgacagcg tgaagctcgg caaggccgtg ctttccgaac tgggcagcgt caacaacctg
     4621 caccggcact cggtggaaca ggccggattc gccgtgctga aagcgccgga tattccctcg
     4681 atcctggtgg aaaccgcatt catcagcaac cctgaagaag agattagact caaagaagaa
     4741 agctaccaga acaaaatcgc cgacgcgatt gtcggcggca tcaaacgcta tttcgtcaag
     4801 aacccgccgc tcgccaaatc caagctcgcg cggtattaaa gcgaccgggg gctaaatttc
     4861 agcagtacag acgactctct gacacctgac gagagccttt cccgcagctt tgtgaactgc
     4921 ttcacacctt ccacttcaag cggatcgtgg ataaacgttc gtcggcctga aggtcttgtt
     4981 tttaaaataa aatcattgaa agcggccctc cctggcacag cggttgctgc gtaaagggca
     5041 agagaaatca gtaagtggtt tcgatgcgct taacggaaag gaggtgtgcc atgttggcga
     5101 aattgaatcc tgtgtatgca aagatcgcgg tggcgctgat tatttcggtg ctggctttca
     5161 ggctcggcgg cttagccggg cacactgaat accggttacc cgcagccacc cagtccgaat
     5221 gctcccgttg attcaaccac ggggcaatga gtagctaggt ttcctcctga agcagggcgg
     5281 gtcgtgaaag agttgtaaag gggaccgccc gccctgctct ttttttacag cgccttgacg
     5341 atatcctcga ctactttctt cgcgtcgccg a
//
LOCUS       DATIOW010000045         2802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_119282, whole genome shotgun sequence.
ACCESSION   DATIOW010000045 DATIOW010000000
VERSION     DATIOW010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119282"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VLL03_01385"
     CDS             complement(<1..753)
                     /locus_tag="VLL03_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HSS46053.1"
                     /translation="MKHINKSFSYGQHQVTLETGEVARQATAAVMVNMDDTVVLVTVV
                     AAKEAMPDQDFFPLTVDYQERTYAAGKIPGGFFKREGRPSEKETLTSRLIDRPIRPLF
                     PDGFHNEVQVIATVMSSNNEVDSDVPAMLGASAALAISGIPFHGPIGAARVGYVNGEY
                     ILNPTATQLKSSSLDLVVAGTEQAVLMVESEARELPEDVMLGAVVYGHKEMQAAIRAI
                     NELADEAGAPLSDRQLPAKDEALIAEVTELAEN"
     gene            complement(818..1087)
                     /gene="rpsO"
                     /locus_tag="VLL03_01390"
     CDS             complement(818..1087)
                     /gene="rpsO"
                     /locus_tag="VLL03_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HSS46054.1"
                     /translation="MSVSTAQKAQVLKDYQTANGDTGSTEVQVALLTARINDLTEHFK
                     THVKDHHSRQGLLRMVNRRRKLLDYLKGNDEERYRTLIERLDLRK"
     gene            complement(1197..2108)
                     /gene="truB"
                     /locus_tag="VLL03_01395"
     CDS             complement(1197..2108)
                     /gene="truB"
                     /locus_tag="VLL03_01395"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HSS46055.1"
                     /translation="MQTTRDKRAVSGVILLDKPRDITSNSALQKVKRLFQAAKAGHTG
                     TLDPLATGLLPICLGEATKFSQSLLDAAKAYRAVVKLGQITTTGDAEGEITSTRNVAV
                     SRPQVEAALERFIGNTSQTPPMYSALKHQGRPLYALARQRKSVERPLRQIQITSLHLD
                     AFASDALTITVCCSKGTYIRVLAEDIGGVLGCGAYLQALRRLGVGPFSLDQTVTLAQL
                     EAMSFEERDARLLPVDALLYGLPEICLDELSSGYFLRGHEVAKDDLPVAGLARIYDIR
                     QRFIGVGEITQEGRVRPKRLLASGALK"
     gene            complement(2394..2756)
                     /gene="rbfA"
                     /locus_tag="VLL03_01400"
     CDS             complement(2394..2756)
                     /gene="rbfA"
                     /locus_tag="VLL03_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HSS46056.1"
                     /translation="MHKDYPRSRRVAEQIQRELAEIIRLELKDPRVGMITLTGVEVTP
                     DYAHARIFFTQLGESKPETLQGLQRAAGFLRSQLAQRLKMHAVPQLHFVYDTSVERGV
                     RLSSLIDQAIAEDKRHPS"
BASE COUNT          561 a          823 c          791 g          627 t
ORIGIN      
        1 gttttcggca agctcggtga cctcggcaat cagcgcctcg tctttcgccg gcagctgccg
       61 gtcggacagc ggcgcgccgg cttcatctgc gagttcgttg atagcccgaa tcgccgcctg
      121 catttccttg tggccgtaca ccaccgctcc tagcatcacg tcctcgggaa gctcgcgcgc
      181 ttccgattcc accattagca ccgcctgctc ggttcccgcc accaccaggt ccaacgacga
      241 ggatttcaac tgtgttgccg tgggattcaa tatatattcg ccgttgacat agcccacgcg
      301 cgccgcaccg atggggccgt ggaagggaat gccggaaatc gccaacgcgg cggaagcacc
      361 gagcatcgcc ggaacgtccg aatccacttc gttgttggag gacatcacgg tggcgatgac
      421 ttgcacttcg ttgtgaaagc cgtcgggaaa cagcggcctg atcggccggt ctatgaggcg
      481 cgaggtcagc gtctccttct cggaaggcct gccttcgcgc ttgaaaaaac cgccgggtat
      541 ttttccggcg gcgtaggtgc gttcctggta atcgactgtg agcggaaaga agtcctggtc
      601 tggcatggcc tctttggcgg ccaccacggt aaccagcacc accgtgtcgt ccatgttgac
      661 catcacggcg gcggtggcct ggcgggcgac ttcgccggtt tccagcgtca cttgatgctg
      721 gccgtaacta aagcttttgt tgatgtgctt caaggcgcgt tatcctttgc gaagttctgc
      781 gtgaacccaa gcggcgccgg aatggcggcg ggccgagcta tttgcgcaag tcgaggcgct
      841 cgatcagggt gcggtagcgc tcctcatcgt tgcccttgag ataatcgagc aacttgcggc
      901 ggcgattgac catacgcagc agcccctggc gggaatggtg atccttgacg tgcgtcttga
      961 aatgctcggt cagatcgttg atgcgggcgg tcaacaacgc aacctgcacc tccgtggagc
     1021 cggtatcgcc attggctgtt tgataatcct ttaaaacctg cgctttttgt gcggtactca
     1081 cagacattgc tttctctcca atccaaagac atctcatttt accttttaat cgcctagttg
     1141 ctcaagtgag tatggctttc ctccactgat taaattcgcc tgattccagg cccggtttat
     1201 tttaaagcgc cgctcgccaa caggcgtttg gggcgaaccc tgccctcttg cgtgatttcg
     1261 cctactccaa taaaccgttg tcttatatca taaattcttg ccaggcccgc cacgggcaaa
     1321 tcatctttgg ctacttcatg gccgcgcagg aaataaccgg atgacaattc atccaggcaa
     1381 atttccggca aaccatacaa caaggcgtcc actggcagca gtcttgcatc ccgttcttca
     1441 aagctcatcg cctcaagttg agcaagcgtc acggtctgat ccaggctgaa aggccccaca
     1501 ccgaggcggc gcaacgcctg cagataagct ccgcagccca aaaccccgcc gatgtcttcg
     1561 gccagcaccc ggatataggt ccccttgctg cagcaaaccg taatcgtgag cgcatcagaa
     1621 gcaaacgcat cgagatgcaa tgacgtgatt tgaatttgcc gcaacggcct ttcaaccgat
     1681 ttcctctgtc gggccagcgc gtacaacggc cggccctgat gcttcagcgc cgaatacatc
     1741 ggcggcgtct ggctggtgtt accgataaag cgctcgagcg ctgcttcgac ttgcgggcgg
     1801 gaaaccgcta cgttccgggt tgaagtaatt tctccctccg catcgccggt agtggtgatc
     1861 tgtccgagct tgaccaccgc tcgataggct ttggctgcgt cgagcaaact ttgcgaaaat
     1921 ttggtcgcct cgcccaggca aatcggcaag agaccggtcg ccagcggatc cagcgtgccg
     1981 gtatgcccgg ctttggccgc ctgaaacagc cgctttactt tctgcaaagc cgaattggaa
     2041 gtaatgtccc gcggcttgtc gaggagaata acgccgctga cggctctctt atcgcgcgtg
     2101 gtctgcaaga tgaattaggg aacctctgat taagtcctcc gcggattgcg ttgcaggtaa
     2161 aatgcggagg gatgcgaggc gcgcgacgaa ggtcgtagct tggactacga cgccgaggag
     2221 cgcaacgaca cagacaccgc attttaccgc aacccggagg gacggggctt tccgggcggt
     2281 ctgctttgtc gctcggcttg ccaagggggc attgccattg gctgcgcctc gcttcgcgca
     2341 tcccatcccg gaaaaccgcc gtcgcaaccg cgtgggactt aaccagaggt tccttaagaa
     2401 ggatgccttt tgtcttctgc gatggcctgg tcaatcaggc tggaaagccg cacgccgcgc
     2461 tccaccgaag tgtcgtagac aaaatgcagc tgcggcacgg cgtgcatttt aagccgctgc
     2521 gccagttggc tgcgcaaaaa ccccgccgcg cgttgcaagc cttgaagcgt ttccggtttt
     2581 gattcaccaa gctgggtgaa aaatatcctg gcgtgggcgt aatcgggagt gacttcaacg
     2641 ccggtgagcg taatcatgcc cacgcgcgga tccttcaatt ccagccgtat gatttccgcc
     2701 agttcgcgct ggatttgctc agcgacacgg cggctgcggg ggtaatcctt gtgcatgaca
     2761 aattctggtt gagagttcag agttcaaagt ttagagtaaa ct
//
LOCUS       DATIOW010000046         2744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1542897, whole genome shotgun sequence.
ACCESSION   DATIOW010000046 DATIOW010000000
VERSION     DATIOW010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542897"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..1400
                     /locus_tag="VLL03_01405"
     CDS             84..1400
                     /locus_tag="VLL03_01405"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HSS46057.1"
                     /translation="MKPINVGLLGIGTVGGGTFTVLRRNREEITRRAGRAIVLKMIAD
                     KDLKKARKLAGNSAIVTADALDVVNHPDIDIVLELIGGTTVAKQFILKAIKNGKHVVT
                     ANKALLAKHGNEIFSAAHKHGVMVAFEAAVAGGIPIIKALREGLTANRVEWIAGIING
                     TSNYILSEMRNTGASFAEALRQAQALGYAEADPTFDIEGIDSAHKLTILAAISFGIPM
                     QFDKVYTEGISELTREDIRYADELGYRIKLLGITKRTLKGIELRVHPTLIPARRLIAN
                     VEGVMNAVLVKGDAVGATMYYGAGAGAEPTASAVVADLVDVTRMHTADRKHRVPHLAF
                     QPDALTDTPILPMSEVQTSYYLRLRAFDKPGVLADITRILADSGISIDAMVQKEPSEG
                     EEQVDIIMLTHETVEKHINAAIRKIERLSTISGKVVRIRLEELNRN"
     gene            1631..>2744
                     /gene="thrC"
                     /locus_tag="VLL03_01410"
     CDS             1631..>2744
                     /gene="thrC"
                     /locus_tag="VLL03_01410"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004674289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HSS46058.1"
                     /translation="MSNRYNGVIERYRERLPVDENTPVVSLCEGGTKLIELINLPRLI
                     NKKVRLYAKFEGLNPTGSFKDRGMTLAVSKAVEAGVKAIICASTGNTSAAAAAYAARA
                     GIACFVVIPEGKIALGKLSQAMMHGSLVIQIKGNFDDGMKLVQEIAATLPVTLVNSVN
                     PYRLQGQKTAAFEIVEELGDAPDYHALPVGNAGNITAHWIGYSEAVCENTAACAFCNG
                     QCAYHKGKIATHRPIMLGYQASGAAPFLRGGPLKNPETVATAICIGNPVSWQQAWKVK
                     EESGGWFAECTDEEILRVQKLLAQVEGVFCEPSSATAVTGVLKDLEQGRIGEGAVIVC
                     TLTGHGLKDPDTAIKQSGKPVNVEATREAIEKVIAAN"
BASE COUNT          648 a          754 c          812 g          530 t
ORIGIN      
        1 agaagtaagg agtaaggtgt gggttcgaga tggatttttc tcctcactcc tgactcctca
       61 ccccttacct gagcgaagcg aatatgaagc caattaatgt aggactgctg ggcatcggca
      121 ccgtaggcgg tggaactttc actgtattgc ggcgcaatcg ggaggaaatc acccggcgcg
      181 ccgggcgcgc catcgttcta aaaatgatcg ccgacaagga cttgaaaaaa gcacgcaagc
      241 tcgccggcaa cagtgccatc gtgacagcgg atgccttgga cgtggtaaat catcccgata
      301 tagacatcgt gttggaactc atcggcggta ccaccgtggc gaagcaattc atcctgaaag
      361 caattaaaaa cggcaagcat gtggttaccg ccaacaaggc gctcctggcc aaacacggca
      421 acgaaatttt ctccgccgcg cacaaacacg gcgtgatggt ggcgtttgaa gcggcggtgg
      481 cgggcggcat ccccatcatc aaggcgttgc gcgaaggctt gaccgcgaac cgcgtggaat
      541 ggattgccgg catcatcaac ggcaccagca attacattct ctctgaaatg cgcaatacgg
      601 gcgcaagctt tgccgaggcg ttgcggcagg cgcaggcgct gggttacgcc gaagccgacc
      661 caactttcga catagagggc atagactccg cgcacaagct caccattctg gcagcgatca
      721 gcttcggcat tccgatgcag ttcgacaagg tctacaccga aggcatctcg gaactcaccc
      781 gcgaggatat ccgttatgcc gacgagctgg gctaccggat taaactcctc ggcatcacca
      841 aacgcacgct gaaaggcatt gaattgcgcg tgcacccgac gctgattccg gcgcggcggc
      901 tgatcgccaa cgtcgaaggc gtgatgaacg cggtgctggt aaaaggcgac gcggtaggcg
      961 ccaccatgta ttacggcgcg ggagcggggg ccgagcccac cgcatcggcg gtggtggcgg
     1021 acctcgtgga cgtgacccgc atgcacaccg cagaccgcaa gcaccgcgtg ccacatctcg
     1081 cgtttcagcc ggatgcgctc accgacacgc ccatcctgcc gatgtcggaa gtgcagactt
     1141 cttattatct gcgcttgcgc gccttcgaca agccgggcgt gctcgccgac atcacccgca
     1201 ttcttgccga ctcgggaatc tcgatagacg ccatggtgca aaaggagccg agcgaaggcg
     1261 aagagcaggt ggacatcatc atgctcaccc atgaaaccgt ggagaagcat atcaacgccg
     1321 caataaggaa aatcgagagg ctttccacca tttcaggcaa agtcgtccgc attcgcctcg
     1381 aagaattgaa caggaattga gtttgaatcg cagagatgct gaggcgcaga gaaaaattaa
     1441 gtattagtta ggttgatccc tccgcgtctc tgcgcctctg cggtaaatag ggttttaatt
     1501 cgcctggatg agttgaatag aaattagaat ctttaaccgc ggagtacgcc gaggacgctg
     1561 aggaaaacca gaataattcc gtcttcctct gcgctctctg cgtcctctgc ggtgaaaaag
     1621 ggttttaggt atgtccaatc gttacaacgg agtcatcgag cgctaccgcg agcgcctgcc
     1681 ggttgatgaa aatacgccgg tagtttcact atgcgagggg ggcaccaagc tcatcgaact
     1741 tataaacttg ccgcgcttga ttaacaagaa agtacggctc tacgccaagt tcgaaggctt
     1801 gaatcccacc ggttcgttca aggaccgcgg catgacactg gcggtgagca aggcggtcga
     1861 agccggagtc aaagctatta tctgcgcctc aacgggcaat acttcagcgg cggcagcggc
     1921 gtatgcggcg cgcgcgggca ttgcgtgttt tgtggtgatc cccgaaggca agatcgcttt
     1981 gggtaagctt tcgcaggcga tgatgcacgg ctcgctggtg attcagatca agggcaattt
     2041 cgacgacggc atgaagctgg tgcaggaaat tgcggcgaca ctcccggtca cgctggtgaa
     2101 ttcggtcaat ccctaccgct tgcaagggca gaaaaccgcg gcgtttgaaa tcgtcgagga
     2161 acttggggac gcgccggatt accatgcact gccagtgggc aacgccggta acatcaccgc
     2221 gcactggatt ggttactccg aagccgtctg cgagaacacc gccgcctgtg ctttttgcaa
     2281 cggccaatgc gcttatcaca aaggaaaaat cgcgacccat cgccccatca tgctgggcta
     2341 ccaggcttcc ggcgccgcgc ctttcctgcg cggaggcccg ttgaaaaatc cggaaaccgt
     2401 ggcgaccgct atctgcatcg gcaacccggt gtcgtggcaa caggcgtgga aagtgaagga
     2461 agaatccggc ggctggtttg ccgaatgcac cgatgaggaa attcttcgcg tacaaaagct
     2521 gctggcgcag gtggagggcg tgttctgcga gccttcttcc gcgactgcgg tgactggtgt
     2581 gctcaaggat ctggagcaag ggcgaatcgg cgaaggcgcg gtgattgtct gtaccctcac
     2641 cggccatggg ctgaaagacc cggacactgc gatcaagcaa agcggcaagc cggtcaacgt
     2701 tgaagcgact cgtgaagcga ttgaaaaagt gattgccgcg aata
//
LOCUS       DATIOW010000047         3798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_628612, whole genome shotgun sequence.
ACCESSION   DATIOW010000047 DATIOW010000000
VERSION     DATIOW010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628612"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="VLL03_01415"
     CDS             <1..1299
                     /locus_tag="VLL03_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HSS46059.1"
                     /translation="VEAIGHAIGSEARARKQKTIAVGRDGRLSGPELSAALARGIQKS
                     GINVIDIGMVTTPILYFATHHLQTHSGVMVTGSHNPPDYNGIKIVLSDETLSGESIRK
                     LKTRLDKGDLTQGSGGYQQKNIAEDYLNLIAGDIKLARRMNIVVDCGNGAPGAFAPEL
                     YRKLGCEVKELFCEVDGNFPNHHPDPTQPDNLKDVIQALQTSDAEIGLAFDGDGDRLG
                     VVTKDGKIIYPDRQLMLFAADMLSRNPGAEIIFDVKCTRNLFSWIRERSGKPILWKTG
                     HSLIKKKMQETGALLAGEMSGHIFFKERWYGFDDGLYAGARLLEYLSNQPDISATLNN
                     LPDSVNTPELHLQLKEGENYALIEKLQKTARFDSAKEVITIDGLRVEYADGFGLARSS
                     NTTPVIVLRFEADNQAALKRIQEDFRRVILQAKPGAALPF"
     gene            complement(1399..1986)
                     /locus_tag="VLL03_01420"
     CDS             complement(1399..1986)
                     /locus_tag="VLL03_01420"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HSS46060.1"
                     /translation="MGEANNLIVTRCAWVKQDPLYLNYHDREWGVPVHYDRRLFEFLI
                     LEGAQAGLKWILILRKREAFRQAYDNFNPVKIARYGEEKINALLADTSIVRNRAKIEG
                     AITNAQAFLAVQEQYGNFDEFIWQFVGGKPIVNHWRSVNQIPSSTVHSDAMSKALRKH
                     GFKFAGTTICYAFMQAVGMVNDHTISCFRHDEVSG"
     gene            complement(1965..>3798)
                     /gene="alaS"
                     /locus_tag="VLL03_01425"
     CDS             complement(1965..>3798)
                     /gene="alaS"
                     /locus_tag="VLL03_01425"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HSS46061.1"
                     /translation="ARATHTKDLSSNSLKVIADHIRACGFLIVDGVIPGNEGRGYVLR
                     RIIRRAIRHGYKLGQKQPFFHLLVADLVKAMGAAYPELVSGAERATQVLKQEEERFAE
                     TLENGMEVLEAALHAEDRMLDGETVFKLYDTYGFPLDLTADIARERGVTVDFAGFEAA
                     MERQRERARAASKFGVQGGVEYNGKQTEFHGYETLKLNSRVVALYVDGASVDRTAQGQ
                     HAIVVLDRTPFYAESGGQVGDRGVLIGLATPRATFKVEDTQKIQAEVYGHHGTLISGE
                     LRIGDKVSAEVDMQLRSRTMHNHSATHLMHSALRQVLGTHVLQKGSLVDEFKTRFDFS
                     HPQPVTPDQIREVENLVNREIRHNEPAEARIMKYNEAIKFGAMALFGEKYGDEVRVMS
                     MGNFSTELCGGTHVKRTGDIGFFKIVSETGVAAGVRRVEAVTGEGALEYVQQQEARML
                     EAAAALKAQPQELTQKINQIIENVRALEKELGRLKYKLASSHGEELINQAVEVKGVKV
                     LAATLEGADAKTLRETVDKLKDKLNSCVVVLGSAEGGKVTLIAGVTQDLISKIKAGDL
                     VNHVAQQVGGKGGGRPDMAQAGGIEPDKLPQALASVPQWVKQII"
BASE COUNT          803 a         1113 c         1029 g          853 t
ORIGIN      
        1 gtcgaagcca taggccacgc cataggctct gaagcgcggg cgcgcaagca aaaaactatt
       61 gccgtcggac gagacggtcg cctttccgga ccggaactct cggccgcttt ggcgcgcggc
      121 attcagaaaa gcggcatcaa cgtgatagac atcggcatgg tgacgacacc gatactctat
      181 ttcgcaaccc accatttgca aacccattcc ggcgtgatgg taacgggctc gcacaatccg
      241 cctgattaca acggcatcaa aatcgtgctg agtgacgaaa cgctctccgg cgaatccatc
      301 cgaaaactaa agacgcggct cgataagggc gatctcactc aaggcagtgg cgggtaccaa
      361 cagaaaaaca tcgcggagga ttatttaaac ctcattgccg gcgacatcaa gcttgcgcgg
      421 cgaatgaata tcgtggtgga ctgcggcaac ggcgcgcctg gagcgtttgc gcccgagctt
      481 taccgcaagc tgggctgcga agttaaagaa ctcttttgcg aagtggatgg caattttccg
      541 aaccaccatc ccgatcccac acaacccgac aaccttaaag atgtcattca agccctgcaa
      601 acatccgatg ccgagattgg attggcgttt gacggcgacg gtgaccggct gggcgttgtc
      661 accaaggacg gaaaaatcat ttatcccgac cgccagctga tgcttttcgc cgccgacatg
      721 ctctctcgga atcccggcgc ggaaatcatt ttcgatgtga aatgcacccg caatctgttt
      781 tcctggatac gcgagcgcag tggtaaacca atcttatgga aaaccggcca ttcgctgata
      841 aagaagaaaa tgcaggaaac cggcgcgctg cttgccggcg aaatgagcgg gcatattttc
      901 ttcaaggaac gctggtacgg cttcgatgat ggtctttacg ccggcgcgcg gcttttggaa
      961 tatttgagca accagcccga catcagcgcg acgctcaata accttcccga ttcggtgaac
     1021 acgccggaac tgcatctgca attgaaggag ggggaaaact acgcgctgat tgaaaaactg
     1081 caaaaaaccg cgcgctttga cagtgcaaaa gaagtgatta cgattgacgg cctgcgcgtg
     1141 gaatacgccg acggcttcgg cctcgcccgc tcatccaaca ccacgccggt aattgtgctg
     1201 cgcttcgaag cggataacca agccgcacta aaacgcattc aggaagattt tcgccgcgtg
     1261 attttgcaag ccaagcccgg tgcagcttta cctttctaac aggccgataa aattaacctt
     1321 gtcgttgcaa ggtgcgccct ttgatattgc ccagtagtcc caaagggtcg ggggagaatt
     1381 tttcaggaca aaggtacctc aacctgatac ctcatcatgc cggaagcagc tgatggtgtg
     1441 gtcattcacc attcccacag cctgcatgaa ggcgtagcaa atcgtggtgc cggcgaattt
     1501 gaagccgtgt ttccgtagtg ccttgctcat agcgtcggaa tgcacggtcg aagatggaat
     1561 ttggttcact gagcgccagt ggttgacgat gggcttgccg ccgacaaact gccagatgaa
     1621 ttcatcgaag ttcccgtatt gctcctgaac ggccaggaac gcctgagcat tggtaattgc
     1681 gccttcaatt ttcgcgcggt tgcgcactat gctggtatca gcaagcagcg cgttgatttt
     1741 ttcttctccg tagcgcgcga tttttacagg attaaaatta tcgtacgcct ggcggaaggc
     1801 ctcgcgtttg cgcaggataa ggatccattt caagccagcc tgcgcgccct ccaggatcag
     1861 gaactcgaag agtctgcggt cgtaatgcac cggcacaccc cattcccggt cgtggtaatt
     1921 gaggtagagc gggtcctgct ttacccaggc acagcgcgtg acaattaaat tatttgcttc
     1981 acccattggg gcacgcttgc gagcgcttgc gggagcttgt cgggctcgat accacccgcc
     2041 tgtgccatgt ccggccgtcc gccgcctttg ccgccgactt gctgcgcgac atgattaacc
     2101 aaatcgcccg ctttgatttt agaaatcaga tcttgggtca cgccggcaat cagcgtgacc
     2161 ttgcctccct cggctgaacc taataccacc acacaggaat tgagtttgtc cttgagcttg
     2221 tccacggtct cgcgcagggt tttggcgtct gcgccttcaa gtgtcgccgc cagcactttc
     2281 acgcctttga cttccaccgc ttgattgatg agctcttcgc cgtgcgagga agcaagtttg
     2341 tatttgagac gaccaagttc cttttccagc gcgcggacgt tttcgataat ctgattgatt
     2401 ttttgcgtga gctcctgcgg ctgcgctttc agcgctgctg cggcttccag catccgcgct
     2461 tcctgctgct gcacatattc cagcgcgcct tcgccggtca ccgcttcgac gcggcggaca
     2521 cccgccgcca cgccggtttc agaaacaatt ttgaagaaac cgatgtcgcc ggtgcgcttg
     2581 acatgcgtgc caccgcacag ctcggtggaa aaatttccca tgctcatcac tcgcacttca
     2641 tcgccgtatt tttctccgaa cagcgccatc gcgccgaatt taatggcttc attgtatttc
     2701 atgatgcgcg cctcagccgg ttcgttgtgg cggatttcgc gattcaccag attttccact
     2761 tcgcggattt gatccggcgt caccggctgt ggatgggaaa aatcgaagcg cgtcttgaat
     2821 tcatccacca gcgaaccctt ttgcaaaaca tgcgtgccca atacctgcct cagcgccgaa
     2881 tgcatcagat gcgtcgcgga atggttgtgc atggtgcggc tgcgcagctg catgtccact
     2941 tccgcgctca ctttgtcgcc gatgcgcagt tcgccgctga ttaacgtgcc gtggtggccg
     3001 tatacttcag cctgaatctt ctgcgtatcc tccaccttga aagttgcgcg cggcgtcgcc
     3061 agaccaatca gcacgccgcg gtcgcccacc tgaccgcccg attcggcgta gaaaggcgtg
     3121 cggtcgagca ctacgatggc atgctggcct tgcgcggtgc ggtcaaccga agcgccgtcc
     3181 acgtatagcg ccaccaccct ggaattcagc ttcaaggtct cgtagccgtg gaattcagtt
     3241 tgcttgccat tgtattccac gccgccctgc acaccgaatt tggacgcggc gcgggcgcgc
     3301 tcgcgctggc gctccatcgc cgcttcgaaa cccgcgaaat ccacggtgac gccgcgctca
     3361 cgtgcgatgt ctgctgtaag gtcaagcggg aaaccgtagg tgtcatacag cttgaacacc
     3421 gtctcgccgt ccagcatacg gtcctcggcg tggagcgcag cttccagcac ttccatgccg
     3481 ttttccaggg tctcggcgaa gcgctcttct tcctgcttca gcacttgggt tgcgcgttcc
     3541 gcaccgctga ccaattccgg ataggccgcc cccattgctt tcaccagatc ggcgaccagc
     3601 aagtggaaga aaggctgctt ctgaccgagc ttgtagccgt gtcggatggc gcggcggatg
     3661 atccggcgca gcacgtagcc gcgtccttcg ttgccgggaa ttacgccgtc cacgatgaga
     3721 aaaccacacg cgcggatatg gtcggcgatg accttgagcg aattgctgga aaggtctttg
     3781 gtatgcgtgg cgcgcgcc
//
LOCUS       DATIOW010000048         7997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_629392, whole genome shotgun sequence.
ACCESSION   DATIOW010000048 DATIOW010000000
VERSION     DATIOW010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7997
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629392"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VLL03_01430"
     CDS             complement(<1..266)
                     /locus_tag="VLL03_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46062.1"
                     /translation="MDQQTRQVIKFITAIVCFSLALAGVVGLAYKIFKPEGWLAQAVA
                     QVWDLQMNFSYLTIPVLLGVIALGSYLFSDIVQQKSKLGTVITYT"
     gene            complement(394..1074)
                     /gene="lolD"
                     /locus_tag="VLL03_01435"
     CDS             complement(394..1074)
                     /gene="lolD"
                     /locus_tag="VLL03_01435"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041792630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HSS46063.1"
                     /translation="MNETVLSCCNLQKSYYQGELEVPVLLGVNLEVGHSERVAVVGAS
                     GSGKSTLLHLLGGLDDATSGAVLINGQDIQHLSDAERGRIRNQSLGFVYQFHHLLAEF
                     SALENVAMPLLIRGLSTADANRRASEVLQQVGLSHRLSHRPGELSGGERQRAAVARAL
                     VTKPACVLADEPTGNLDRHNAQSVLELMLELNRSLGTSFVIVTHDHDLAARAERVLRL
                     VDGVLIDG"
     gene            complement(1107..2354)
                     /locus_tag="VLL03_01440"
     CDS             complement(1107..2354)
                     /locus_tag="VLL03_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HSS46064.1"
                     /translation="MVQPYELLIGLRYSRAKRRNHFVSFISLISIIGMALGITVLISV
                     MSVMNGFQKEIRERILGVVSHVQVSGVNGRLNDWHKVAAEAGKNPQVIASAPYVAEQG
                     LLSFEQAVKGTIVRGILPEYENRVVDLGQRMKAGRLDALRPGEFGIVLGVDLARTLGA
                     TVGDKVLLIAPQGQVTPAGILPRLKQFTVVGIFEVGHYEYDAGLALIHLEDAQKLYRF
                     DGNVSGVRLRLKDLYQAPRVANELAHTLTVDAYINDWTRINANFYRAVQIEKRMMFFV
                     VILIVAVAAFNIVSTLVMAVTDKQSEIAILRTLGASPRSIMKIFIVQGTLIGILGTLL
                     GLIGGVVLALNIDVVVPFIENLIGVKFLAKDVYYISELPSDVQFWDVAVIAVVSFALC
                     LLATLYPSWRAARSNPAEALRYE"
     gene            2543..3559
                     /locus_tag="VLL03_01445"
     CDS             2543..3559
                     /locus_tag="VLL03_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46065.1"
                     /translation="MHCHLLIPALLKPAASTNGLYENLSAPALRTLLARATPARELEA
                     DMESWLCHAFGAEKQQDWPVAALALAADGINPGAAYWLRADPVNLQVDRGQLLLMDSR
                     AFPIAQDEADQFIRVLNSQFADRGYVFQAPHPQRWYLRLDKAPNLQTSPLDRVVGKNV
                     DDFLAMGADGLHWQGVCNEAQMLLHEHPLNEVREASGELPINGIWLWGGGHLPMLSRK
                     PFTQVWADGHLPRSLALASAIPWASLPRDAEKWLEQMPVQDEPLIVLDQLLSAALYHD
                     SERWRSGVKELEISWFAPLLAALKSGRVAAIIIVADGSSFSIMRKDSWKWWRRSRPFA
                     EYTH"
     gene            3562..5265
                     /gene="recJ"
                     /locus_tag="VLL03_01450"
     CDS             3562..5265
                     /gene="recJ"
                     /locus_tag="VLL03_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HSS46066.1"
                     /translation="MSAPKIVYRSFPQQAYQTLLAEGVHPVLARIYAARGIVAKAQLA
                     GELSGLAACDRLKNATRMAEILADAITAKKQLLIIADYDADGATACAVGLRALREFGA
                     TVDYLVPNRFEYGYGLTPEIVRLAAQTRRPDILITVDNGIASVEGVAEAAGLGMETLI
                     TDHHLPGSTLPQAASIVNPNQPGCGFPSKNLAGVGVMFYVMLALRAELRLRGAFAAKP
                     EPNLSTLLDLVALGTVADVVKLDDNNRILVSQGLKRIRAGKACAGVQALFRAAGRDAR
                     RASTYDLGFMLGPRLNAAGRLSDMALGISCLLTDDAIRAEEIARQLDVLNRERREIEQ
                     DMREAALAALEKMEFRESYSVCLYDPGWHQGVVGILASRIKDRLHRPVIAFARGNDGE
                     IKGSGRAIKGLHLRDALDLVTKRHPGLILKFGGHAAAVGLTLYEKDFESFARIFEETV
                     QTLLTPADLERVIEIDGALGAPELHIEFARLLEHETWGQGFPAPLFDDAFAVENQRVV
                     GEKHLKLKLRKADRVLEAMLFQHADPLPDRIHAVFRLTVNEYSGLQTLQLNIEHWTPA
                     P"
     gene            5293..6397
                     /gene="prfB"
                     /locus_tag="VLL03_01455"
     CDS             join(5293..5373,5375..6397)
                     /gene="prfB"
                     /locus_tag="VLL03_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417196.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HSS46067.1"
                     /translation="MEAEQLNAIANRLTDLDVRAAELRRYLDFEAKQERLNEVVKLSE
                     DPAVWSDNKRAQELGKERKALENTVGTLSKLTRSLADARELFDMAKAENDDAALESIR
                     VETVELEQTVTEMEFRRMFSNPMDPNNCFLMIQAGSGGTEAQDWAAMLERMYLRYSER
                     HGFRVELLEESEGEVAGIKSATMKINGDYAYGRLRTETGVHRLVRKSPFDAGNRRHTS
                     FASVFVYPEVDESIEVEINPADLRVDTYRASGAGGQHVNKTDSAVRLTHIPTGIAVQC
                     QNDRSQHRNRAEAIAMLKSRLYELELRKRQAERDKLEDAKTDIGWGHQIRSYVLDQSR
                     IKDLRTGVEIGNTQAVLDGNLDDFITASLKQGV"
     gene            6398..7903
                     /gene="lysS"
                     /locus_tag="VLL03_01460"
     CDS             6398..7903
                     /gene="lysS"
                     /locus_tag="VLL03_01460"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HSS46068.1"
                     /translation="MSEKNDEKNLDTNQIIEERREKLKALRGKGPAFPNDFRREHLAD
                     ELHARHGSKSHEELEAAHIKVSVGGRMMLKRVMGRASFATIQDMSGRLQLYITNDHAG
                     ESAHEAFKHWDLGDIIGAKGTLFKTKTGELSVRVYELRLLTKSLRPLPEKFHGLTDQE
                     SKYRQRYVDLIINEETRKVFSIRCKIMQTVRELLVQDGYIEVETPMMQPIPGGAAARP
                     FVTHHNALDLRLYLRIAPELYLKRLVVGGMEKVFEINRNFRNEGISTRHNPEFTMLEF
                     YCAYQDYRYLMDFTESMLRILAQAVLGSSTINYQGQEIDFGRPYPRLTITQAIRKYHP
                     EYTDAKLKDREFLVGEFKKRDIAYKPGDGIGGLQLTLFEHTTEQLLLQPTFIVDYPAE
                     VSPLARRSDNNPEIAERFELYIAGREIANGFSELNDPEDQAARFSEQARQKEAGDQEA
                     MHYDADYIHALEYGLPPAAGCGIGIDRLVMLFTDSPSIRDVILFPQMRPEI"
BASE COUNT         1772 a         2417 c         2274 g         1534 t
ORIGIN      
        1 gtgtaagtga tgacagtgcc caatttgctt ttttgctgaa cgatgtccga gaacagatac
       61 gagcccaatg caatgacgcc gagcagcacc gggatggtca ggtaggaaaa attcatttgc
      121 aagtcccaca cctgggcaac cgcctgcgcc agccagccct cgggcttgaa aatcttgtaa
      181 gccagcccca ccactcccgc gagcgcgagg ctgaagcaca ctatcgcggt gatgaattta
      241 ataacttgac gggtttgttg atccattcac acaccttcgg ttccggtgga agtggcggca
      301 accgctttcg catagaaacc gtcgcaactc tacccgcaaa tgcctgatgc cgcaagcata
      361 agctccgttt accgatcgat attcatctcc ctcctaacca tcaatcagca ccccatccac
      421 tagtcgcaag acgcgttccg ccctcgccgc cagatcgtgg tcgtgggtga caatgacgaa
      481 gctagtgccg aggctgcggt taagctcgag catcagctcg agcacgcttt gtgcgttgtg
      541 ccggtcaagg ttgccggtcg gctcgtccgc gagcacgcat gcaggcttgg tcaccaatgc
      601 gcgcgccacg gccgcacgct ggcgctcgcc acccgatagc tcaccgggac gatggctgag
      661 ccggtggctc aggcccactt gttgcaacac ttcagaagcg cgccggtttg cgtctgccgt
      721 tgacaggcca cggatgagca gcggcatcgc cacgttttct agcgcggaga attccgcaag
      781 caggtgatga aactggtaaa caaagcccag cgattggttg cggatcctgc cgcgctccgc
      841 gtcgcttagg tgctggatat cctgtccgtt gatcagcact gcgccggaag tcgcgtcatc
      901 caatccgccg agcagatgca aaagcgtgct tttgcccgaa ccggaagcgc cgacgacggc
      961 cacgcgttca ctgtggccga cttcgagatt cactccgagc agcaccggca cttccagctc
     1021 accctggtaa taagacttct gcagattgca gcaggataaa accgtttcat tcatgtcctg
     1081 aatcccgaat cccaaatcct gaatcatcat tcatagcgca acgcctccgc cggattgctc
     1141 cgcgccgcgc gccaactggg atataaagtc gccagcaggc acagcgcgaa tgagacaacg
     1201 gcaatcaccg ccacgtccca gaattgcacg tccgacggca attcgctgat gtaatacaca
     1261 tccttggcga ggaatttgac gccgatgagg ttctcgatga acggcaccac cacgtcgatg
     1321 ttaagcgcca gaaccacgcc gccgatgagg ccgagcagcg tgcccagaat gccaatcaac
     1381 gtgccttgca cgatgaagat tttcatgatg ctgcgcgggc tcgcgccgag ggtgcgcagg
     1441 atcgcgattt ccgattgttt gtcggtcacc gccatcacca gcgtggatac gatattaaac
     1501 gccgccaccg cgacgatcaa tatcaccacg aaaaacatca tgcgcttttc aatctgcacc
     1561 gcgcggtaaa agttggcgtt gatgcgcgtc cagtcgttga tgtaggcatc caccgtcagc
     1621 gtgtgcgcga gctcgttcgc cactcgcggc gcctgataca aatccttgag acgcagccgc
     1681 acgccgctga cgttaccgtc gaaacggtag agcttctgcg cgtcttcaag atgaatcagt
     1741 gccagtcccg catcgtattc gtagtgtccg acctcgaaaa tgccgacgac ggtgaactgc
     1801 ttgaggcgcg gcagaattcc cgccggggtc acctggccct gcggcgcgat caaaagcact
     1861 ttgtcgccca cggttgcgcc cagagtgcgg gccagatcca cgcccagcac aatgccgaat
     1921 tccccggggc gcagcgcatc cagccgcccc gctttcatgc gctggcccag atccaccacc
     1981 cggttttcgt attccggcag tatgccgcgc acgattgtgc ccttgactgc ctgttcgaac
     2041 gaaagcaagc cctgctcggc gacatacggc gcgctggcga tgacttgcgg gtttttcccc
     2101 gcttcagcgg cgaccttgtg ccagtcgttg agcctgccgt tcacgccgct cacctgcacg
     2161 tgcgacacca cgccgaggat gcgttcgcgg atttccttct gaaagccgtt catcaccgac
     2221 atcaccgaaa tcagcacggt gatgccgagc gccatgccga tgatggaaat cagcgaaata
     2281 aaggaaacaa aatgattgcg ccgcttggcg cggctgtagc ggaggccgat gaggagttcg
     2341 taaggctgca cgataaaatc aggattcagg agccaggatt aaggattcag ttccaggacc
     2401 ataaaatgcc gtctattgta gcgcaggcgc ggctgacaca tatgactaat catcaagcgc
     2461 cgcctgcgag ttcttgattc ttgccctgtg ctagacttgt ctagatagtc gttgctttac
     2521 gaatcctaat cccgaatcct gaatgcattg ccaccttctt attcccgccc tgcttaagcc
     2581 cgccgcgtca accaatggcc tgtacgagaa cttatccgcg cccgcattgc gcacactgct
     2641 cgcccgcgca accccggccc gcgaattgga agcagatatg gaatcctggc tgtgccatgc
     2701 atttggcgcg gaaaaacagc aagactggcc ggtcgccgca ttggcgctcg cggcagacgg
     2761 catcaatccc ggtgcggctt attggttgcg cgccgacccg gtaaatctgc aagtggatcg
     2821 cgggcaatta ctgctgatgg acagccgtgc tttcccaatt gcgcaagatg aagcagacca
     2881 attcatccgc gtgctcaaca gccagttcgc cgaccggggt tatgtttttc aggcgccgca
     2941 tccgcagcgc tggtacctgc gcctggataa agcccccaat ctgcaaacct cgccgcttga
     3001 tcgggtagtg ggcaaaaacg tggatgattt tctggcaatg ggcgcggatg gcctgcattg
     3061 gcaaggcgtc tgcaacgaag cgcaaatgct gctgcacgag catccgctca acgaggtacg
     3121 cgaagcgagt ggcgaattgc ccatcaacgg tatctggctg tggggaggcg gtcatttacc
     3181 tatgctttcc cggaaacctt ttacccaagt atgggccgat gggcatctgc cgagaagttt
     3241 ggcactcgcc agcgccatac cctgggcttc actccctcgc gatgcggaaa aatggctgga
     3301 gcaaatgccg gttcaagacg agcctttgat tgtgctcgac cagttgctaa gcgcggcgct
     3361 ctatcacgac tccgagcgct ggcgctctgg ggtgaaagaa ctcgagattt cgtggtttgc
     3421 gccgctgctc gccgcactca agagtggccg ggtggcggca attattattg ttgcggacgg
     3481 ctcgtcattc agcatcatgc gcaaagactc atggaaatgg tggcgaagaa gcaggccatt
     3541 tgctgaatat acgcattaat aatgagtgcg cccaaaatcg tttatcgcag ctttccccaa
     3601 caggcctatc aaaccctgct ggcggaaggc gtgcatccgg tgctcgcgcg catttacgcc
     3661 gcgcgcggga tcgttgccaa ggcgcagctc gccggcgagt tatcaggcct cgcggcttgc
     3721 gaccggctga aaaacgcaac gcgcatggca gaaatcctgg ccgacgcgat cacagcgaaa
     3781 aaacagctgc tcatcatcgc cgattacgat gccgacggcg ccaccgcctg cgcggtgggc
     3841 ctgcgcgcgc tgcgcgaatt cggcgccacc gtggattatc tcgtccccaa ccgctttgaa
     3901 tacggctatg gcctcacgcc ggaaatcgtg cggctcgcgg cgcaaaccag gcgtcccgac
     3961 atcctgatca ccgtggacaa cggaatcgcc tcagtcgaag gggttgcgga agccgcaggg
     4021 ctgggaatgg aaacgctgat taccgaccat cacctgccag gaagcacact gccgcaggcg
     4081 gcttccatcg tgaaccccaa ccagcccggc tgcggattcc ccagcaaaaa cctcgctggt
     4141 gtcggcgtga tgttctacgt gatgctggcg ctgcgcgccg aattgcggct gcgcggcgcg
     4201 tttgccgcga aacccgagcc gaatctttca accctgctcg acctggtggc gctgggcacg
     4261 gtggcggatg tagtaaagct ggatgacaac aatcgcattc tggtgagcca gggcctgaag
     4321 cgcatacgcg cggggaaagc ctgcgccggt gtgcaggcgc tgttccgcgc cgcgggacgg
     4381 gatgcgcgcc gtgcttccac ctatgatctc ggcttcatgc tcggaccgcg ccttaacgcc
     4441 gcgggcaggc tttccgatat ggcgctcggc atttcatgcc tgctcactga tgacgccatc
     4501 cgtgccgagg aaattgcacg gcaactggat gtgcttaacc gcgagcgtcg ggaaatcgag
     4561 caagacatgc gggaagcggc gctggctgcg ctggaaaaga tggaattccg ggaaagctat
     4621 agcgtttgtc tttacgatcc gggctggcat caaggcgtgg tcgggattct tgcctcgcgc
     4681 atcaaggacc ggctgcatcg gccggtgatt gcgtttgcca gaggcaatga cggcgaaatc
     4741 aagggctcgg gtcgcgccat caaggggctg catctgcgcg atgcgctgga tttggtgacc
     4801 aaacgccatc ccggtttgat tttgaaattc ggcgggcacg ccgcggcggt gggcctgacg
     4861 ctatacgaaa aagacttcga gtcattcgcc aggattttcg aggaaaccgt tcagaccctg
     4921 ctcacacccg ccgatctgga gcgggtgatt gaaatcgacg gggcgctggg ggcgccggaa
     4981 ctgcacatcg agttcgctcg ccttttggaa cacgaaactt gggggcaggg tttccccgcc
     5041 ccgctcttcg acgacgcatt cgcggtggag aaccagcgtg tcgtcggtga aaagcacctc
     5101 aaactcaagc tgagaaaagc cgaccgtgtg cttgaagcca tgctgttcca gcacgccgat
     5161 cctctgcccg atcgcatcca cgcggttttc cgtctgaccg tgaacgaata cagcggcctg
     5221 cagacgctgc agctcaatat cgagcattgg acgcccgcgc cgtgataaaa tttacaaatt
     5281 tcaggaatac ccatggaagc cgaacaactc aatgccatcg ccaaccgcct gaccgacctc
     5341 gatgtccggg cagcggaact gcggaggtat ctttgacttc gaagcaaagc aagaacgcct
     5401 gaacgaagtc gtcaaacttt ccgaagatcc ggccgtctgg agtgacaaca agcgcgccca
     5461 ggaactcggc aaagagcgca aggcgctgga aaacacggtc ggcacgctct cgaagctcac
     5521 acgatcgctt gccgatgcgc gcgagctgtt tgacatggcg aaagcggaaa acgatgacgc
     5581 tgcgctggaa agcatacgcg tcgagacggt cgagctggaa cagaccgtta ccgaaatgga
     5641 attccggcgc atgttttcca atccgatgga ccccaacaat tgctttctca tgatccaggc
     5701 cggctccggc ggcaccgagg cgcaggactg ggcggcaatg ctcgagcgca tgtatttgcg
     5761 ctatagtgag cggcatggtt tccgggtgga attgctggag gaatcggagg gcgaagtcgc
     5821 cggcattaag agcgcaacca tgaagataaa tggcgactat gcttacggcc ggctacgcac
     5881 cgaaaccggc gtgcaccggc tggtgcgcaa atcgccgttc gatgccggca accgtcgcca
     5941 cacctcgttc gccagcgtgt tcgtctatcc ggaagtggat gagtccatcg aagttgaaat
     6001 caatcccgcg gatttgcgcg tggacaccta ccgcgcctct ggcgccggcg gccagcatgt
     6061 aaacaagacc gattccgcgg tgcggctcac tcacattccg accggcatcg cggtgcagtg
     6121 ccagaacgac cgttcgcagc accgcaaccg cgccgaggcg atagcgatgc tgaagtcgcg
     6181 cctctatgaa ctggaactac gcaagcgcca agccgagcgc gacaaactgg aagacgccaa
     6241 gactgacatc ggctgggggc accagatccg ctcctacgtg ctggatcaat cgcgcatcaa
     6301 ggacctgcgc accggggttg aaatcggcaa cacccaggca gtgttggacg gaaaccttga
     6361 tgatttcatc accgccagct tgaaacaagg agtgtaaatg tccgaaaaaa acgacgaaaa
     6421 aaatctggat accaaccaaa tcatcgaaga gcgccgcgag aagctgaaag cattgcgcgg
     6481 caaaggccct gccttcccca acgatttccg gcgcgagcat ctcgccgacg aactgcacgc
     6541 gcggcacgga agtaaatccc atgaagagct cgaagccgcg catatcaagg tcagcgtcgg
     6601 cggacggatg atgctgaagc gcgtgatggg aagagcgagc ttcgccacca ttcaggacat
     6661 gagcggccgc ctccagctct acatcaccaa cgaccacgcg ggcgagtcgg cgcacgaagc
     6721 attcaagcac tgggatctgg gcgacattat cggcgccaaa ggcacgctgt tcaaaaccaa
     6781 gaccggcgag ctgtcggtac gcgtttacga attgcgtctc cttaccaaat ccttgcgccc
     6841 gttgccggag aaattccacg gtttgaccga ccaggaaagc aaataccgcc agcgctatgt
     6901 ggacctgatc attaacgagg agacgcgcaa ggtattctcc attcgctgca aaatcatgca
     6961 gacggtgcgc gaactcctgg tccaggacgg ttacattgaa gtggagacgc cgatgatgca
     7021 gccgattcct ggcggagcgg cggcgcgccc tttcgtcacc catcacaacg cgctcgacct
     7081 gcggctttat ttgcgcatcg cgccggagct ttatctcaaa cgcctggtgg tgggcggcat
     7141 ggagaaagtg ttcgagatca accgcaactt caggaacgaa ggcatctcca cgcgccacaa
     7201 tcccgaattc accatgctgg agttttattg cgcctaccag gattaccgct atctgatgga
     7261 cttcaccgaa tccatgctgc gcatattggc gcaagccgtg ctgggctcaa gcacaatcaa
     7321 ttatcaaggg caggaaatcg attttggcag gccttatccg cggctcacca tcacgcaggc
     7381 gattcggaaa tatcatcccg aatacaccga cgccaagctc aaggaccgcg agtttctggt
     7441 cggtgaattc aaaaaacgcg atatcgccta caagcctggt gacggcatcg gcggcctgca
     7501 gcttacgctg ttcgagcaca ctaccgaaca attgctcctc cagccgacct ttattgttga
     7561 ttatcccgcc gaggtttcgc cgctcgcgcg ccgcagcgac aacaacccgg agattgccga
     7621 gcgctttgag ctttacatcg ccgggcgcga aatcgccaac ggtttttccg agcttaacga
     7681 tcccgaagac caggcggcac gtttctcgga gcaagccagg caaaaggaag ccggcgacca
     7741 agaagccatg cactatgacg ccgattacat ccacgcgctg gaatacggcc tgccgcccgc
     7801 cgcaggctgc ggcatcggca tcgaccgtct ggtgatgctg ttcaccgaca gtcccagcat
     7861 tcgcgatgta atcctttttc ctcaaatgcg cccggagatt tagccgggca gaaccttcgt
     7921 gacaacgcct gcgctcctcg tcaacgccac gatcaacacg ccgcgccgcc atccaccttt
     7981 cggcgagatg gacgacg
//
LOCUS       DATIOW010000049         6276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1544393, whole genome shotgun sequence.
ACCESSION   DATIOW010000049 DATIOW010000000
VERSION     DATIOW010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544393"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1177
                     /gene="hemC"
                     /locus_tag="VLL03_01465"
     CDS             221..1177
                     /gene="hemC"
                     /locus_tag="VLL03_01465"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HSS46069.1"
                     /translation="MSNSSFRAAPAKIVIASRESALALWQARFIQGKLCALHPQLQIN
                     IFGMTTEGDRLLDTPLVKIGGKGLFVKELEEALQQGTADIAVHSMKDVPMRLPPGFML
                     AAITEREDARDAFVSNRYPSLEDLPHGSVVGTSSLRRESQLRARFPHLNVTSLRGNIG
                     TRLKKLDQGPYAAIILAAAGLKRLGLEGRITALLSPEQSLPAVGQGALGIECLGKRDD
                     LLQLLQPLNHRATALCVAAERAMSRALGGSCQVPLGGYAQMQGNEIHLRGFVATPDGS
                     RLVSAEARGNAEKAQVLGEALARALKAKGADKILAQLESQGA"
     gene            1174..1986
                     /locus_tag="VLL03_01470"
     CDS             1174..1986
                     /locus_tag="VLL03_01470"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HSS46070.1"
                     /translation="MMDTRPKVSSAARKRVLSDRNILVTRPQNQAQELADLIRAEGGC
                     AILFPALEILEADDPRPLHQLIGRLEQFDLAIFISANAAEKAMGEIVARRSLPDTLKF
                     AAIGSGSRKALQRFGVKEVIYPATRFDSEALLGLPQLRDVAGKRIVIFRGQGGREWLG
                     ETLAKRGARVEYVECYRRAKPASDPAPLERLWADKKLHAITVSSSEGLRNLYEMMGET
                     GRELLRKTPLFVPHHRIAEVARELGLANLVVTAAGDAGLVKGLIDWFGAKAG"
     gene            1993..3027
                     /locus_tag="VLL03_01475"
     CDS             1993..3027
                     /locus_tag="VLL03_01475"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HSS46071.1"
                     /translation="MTDKSQIETLPAPAKPEKRFNLALLIALVVAALLAWQWFDTRSQ
                     FSALQQELGKRLAEADNTVKENRAIVKDTQEALREAQVKLGVLENKLAESQSQQVALE
                     TLYQELSRNRDEWALAEVEQMLNAASQQLQLAGNVKAALIALQTADARLRRIDRPLLI
                     PLRKIINKDIERLKAVPYVDTVAISVRLDNLITKVDSLPLAMDVRLPVNKTAAVEEAA
                     QSAWLKLAREAWQDIKQLVRMQNTAEHEIPLLAPDQLFFLRENLKLRLLSARLALLLH
                     DETSFKSDLIAAQEWLTRYYDNSAEVTKATLASLRQLQESRISIELPDISASLDAARN
                     FKLAREKATK"
     gene            3024..4244
                     /locus_tag="VLL03_01480"
     CDS             3024..4244
                     /locus_tag="VLL03_01480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HSS46072.1"
                     /translation="MKLFFWIVAIFALAVALSLMIGFNNGYVLLVYSTYRIELSFKLA
                     VLLLFGGFFITYVLVRLLVHVLRLPAQVREYRARNRREKARNSLLEGLRMFLEGRYGK
                     AEKAAAAALEMGESPGLTALLAARSAHELKAFDKRDAYLNEAETREPEEKAARLMTQA
                     ELLLDQHRNQDALTVLKDLHDSGSKKHVAALRLELEAQQREKNWGQVLNLVAQLEKRG
                     FFDAGQAQQLRLYATQENLKRQAHNIAALQEFWKKIPTADKKISGIAATAARCFISLG
                     GCRMAQEIIEQSLDAQWDSDLICLYSECLGTDTLRQIERAERRLFDHPYDVCLLLTLG
                     KLCAHQRLWGKAQSYLEASLSVEPTATAHRELAKLHEKIGQSEAADIHYRKSLDLTVR
                     QLHDITGGRRKEAL"
     gene            complement(4341..5372)
                     /locus_tag="VLL03_01485"
     CDS             complement(4341..5372)
                     /locus_tag="VLL03_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HSS46073.1"
                     /translation="MSFQVTIKPSDHAFFVETGEGVLEAALRQGFGLPYGCRNGACGS
                     CKGKILEGKVNYGVYQESALSEAEKLAGMALFCQAKPQTDLVIEAREINAVKDIQIRK
                     LPCRVQKMERPADDVIVLYLKLPANERLQFLAGQYIDILMKEGKRRSFSMANPPHDDE
                     CLQLHVRNIVGGAFTDYVFNKMKEREILRFEGPLGTFFLREDSDKPIILVASGTGFAP
                     IKGIIEHAFYKESKREMVLYWGARKLADLYMLQLPQQWQLEHDNFSFIPVLSEALPED
                     RWQGRSGLVHQAVIDDFADLSSFQVYACGAPVMVEAARRDFTAMRKLSLEEFYSDAFT
                     YSNDPKPAY"
     gene            5433..>6276
                     /locus_tag="VLL03_01490"
     CDS             5433..>6276
                     /locus_tag="VLL03_01490"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSS46074.1"
                     /translation="MRRLLIIGCGDVALRMVRKLPAGYRVYALSHSPERYHLLREHGL
                     TPIPGNLDEPDTLEMLGGLAHDVVHFAPPPSQGTKDTRTAHLLATLTKGKILPHRLIY
                     ISTSGVYGNCDGDIVPETRPLRPHTARAKRRLDAERRIREWGKRSRVCVSILRVPGIY
                     AGDRLPLEHVKNGTPALLPEQDAYSNHIHADDLASIVIAALRFGKPGRVYNTSDDSEL
                     KMGDYFDLVADRFGLPRPTRISYSEARQRIPENLLSFMQESRRLANRRMKRELQVKLR
                     YPVVA"
BASE COUNT         1397 a         1748 c         1894 g         1237 t
ORIGIN      
        1 aaaagtggct gaagaaggcg aggaaacgca ctgggaggtg gtgctcaagg gagtggaaga
       61 aaaactgccg gtcagccgcc gccagcagta ccttgtcaaa gaattcacta gtgaaaggcc
      121 atcctttaaa tacgggcggc acgaagccca ggcgccgtcc gtgttaaaat aaacgcgaag
      181 aataggcatc tgtaatacgc gcgaccggcc ggaaaatatt ttgagcaact cttcgtttag
      241 agcagcgcct gccaaaatcg tcatagcttc gcgcgaaagc gcgctcgcgc tatggcaagc
      301 acggttcatt cagggcaaac tctgcgcgct gcacccgcaa ctccagatca acatattcgg
      361 catgaccacc gagggcgacc gcttgctaga cactccgctc gtcaaaatcg gcggcaaagg
      421 ccttttcgtc aaggaactgg aagaggcatt gcagcaggga accgcggaca tcgccgtgca
      481 ttcgatgaaa gacgtgccga tgcgtttgcc gcccggcttc atgctggcgg cgattaccga
      541 gcgcgaagat gcgcgcgatg cgtttgtgtc aaaccgctat ccgagcttgg aagacctgcc
      601 tcacggcagt gtggtgggaa cttccagctt gcgcagggag agccagctgc gcgcgcgctt
      661 tccacatctc aacgtgactt cgctgcgcgg caatattgga acccgcctga aaaagctcga
      721 ccaagggcct tatgctgcaa tcatactggc ggccgccggc ttgaaacggc tgggactgga
      781 aggacgcatc accgcgctgt tgagccccga acaaagcctg cctgcggtgg gtcaaggcgc
      841 actgggaatc gaatgcttag gcaagcgtga tgacctgctg caacttttgc agccgttgaa
      901 ccaccgcgca accgcgcttt gcgttgccgc ggaacgcgcc atgagccgcg cgctgggagg
      961 cagttgccag gtgccgctgg gcggctatgc acagatgcaa ggcaatgaga tacatttgcg
     1021 cggctttgtt gcgacgcctg acggcagccg cctggtgagc gccgaagcgc gaggcaacgc
     1081 ggaaaaagcg caggtattag gtgaagcatt ggcaagagcg ttgaaagcca aaggcgcgga
     1141 caagatactc gcccagctgg agtcgcaagg cgcatgatgg atacccgacc caaggtttct
     1201 tctgcggcgc gaaagcgcgt gttgagcgac cgcaatatct tggtgacgcg tccgcagaac
     1261 caggcccagg aacttgccga tttaatccgc gccgagggag gttgcgcaat tttatttccg
     1321 gcgctggaaa tcctcgaagc tgatgacccg cgcccgctgc atcaattgat cggccgtctc
     1381 gagcaattcg acttggcgat tttcataagc gccaacgcgg ctgagaaagc gatgggcgaa
     1441 attgtcgcgc gccgcagttt gccggatacg ctcaaattcg cagcgatcgg ctcaggcagc
     1501 cgcaaagcat tgcagcgttt cggcgtgaag gaagtgattt acccggcgac gcgctttgac
     1561 agcgaagctc tgctcggact tccgcagttg cgcgacgtgg caggcaagcg catcgtgatt
     1621 tttcgcggcc agggcgggcg cgaatggctt ggtgagacac tggccaagcg cggcgcgcgc
     1681 gtcgaatacg tggaatgtta tcggcgcgca aaacctgcgt ccgatccggc gccgcttgag
     1741 cggctgtggg cggacaaaaa attgcacgcc attacggtga gcagcagcga agggctgcgc
     1801 aatttgtatg agatgatggg cgaaacgggg cgtgagctgc tcagaaaaac cccgctgttt
     1861 gtgccgcacc atcgtatcgc cgaggtggca cgggagctgg ggctggccaa tctagtggtg
     1921 accgcagccg gcgacgcagg cctggtcaaa ggtttgattg attggtttgg ggcaaaagcc
     1981 ggatagtcag ccgtgaccga taaatcgcaa attgaaacac tgcctgcacc cgccaagccg
     2041 gaaaagcggt tcaacctggc gcttttgatt gcgctggtcg tggcggctct tttggcctgg
     2101 cagtggtttg atacccgcag ccagttcagc gctttgcagc aggaactcgg caagcgcctg
     2161 gcggaagccg acaacaccgt caaggaaaac cgcgctatcg tcaaagacac ccaagaagcg
     2221 ctgcgcgaag cgcaagtgaa actcggtgtg ctcgaaaaca agctcgcgga atcgcaaagc
     2281 cagcaggttg cgctggaaac cttgtatcag gaactttccc gcaaccgtga cgagtgggct
     2341 ctggcggaag tggagcagat gctcaacgcc gcgagccagc aactgcaact ggcgggcaac
     2401 gtaaaggccg cgctgattgc actgcaaacg gcggacgcgc gcctgcgacg catcgaccgc
     2461 ccgctgctca ttccgctgcg caaaatcatc aacaaggaca tcgagcggct caaggcggtg
     2521 ccttacgtgg acacggtggc gatcagcgtg cgactggata acctgataac caaggtggac
     2581 agcctgccgc tggcgatgga tgtccgtctg cccgtgaata aaaccgctgc tgtcgaagag
     2641 gctgcgcaaa gcgcgtggct gaaactggcg cgcgaagcgt ggcaggacat caagcagctg
     2701 gtgcgcatgc aaaacaccgc tgagcacgag atacccctgc ttgcgccgga ccaattgttt
     2761 ttcctgcgtg aaaatctcaa gctgcgcttg ttaagcgcgc ggctcgcttt gctgttacat
     2821 gacgagacca gtttcaagtc tgaccttatc gccgcgcagg agtggctcac ccgctattac
     2881 gacaacagcg ccgaggtgac caaggccacg ctggccagcc tgcgccagct acaggaaagc
     2941 cgcatcagca tcgagcttcc cgacatctcg gcgagcctgg atgcggcgcg caacttcaag
     3001 ctcgctcgcg agaaggcgac aaagtgaagt tgttcttctg gatcgtggcg atttttgcgc
     3061 tggcggtagc tttatccctg atgatcgggt ttaacaacgg ctacgtgctc ttggtctatt
     3121 ccacttaccg catcgaactc tcgtttaagt tggcggtgtt gctcctgttt ggcgggtttt
     3181 ttatcaccta tgtgttggtt cgcctgttgg tgcacgtgct gcgcctgccg gcgcaagttc
     3241 gcgagtaccg cgcgcgcaat cgtcgcgaaa aggcgcgcaa cagcctgctt gagggattgc
     3301 ggatgtttct tgaagggcgt tacggcaagg cggaaaaagc cgctgcggcc gctctggaga
     3361 tgggggaatc tccggggctc accgcgctac tggccgcgcg ctcggcgcat gagttaaaag
     3421 cctttgacaa gcgcgatgcc tacctcaatg aggcggaaac acgggagcct gaagaaaaag
     3481 cggcgcgcct gatgacgcag gctgaactgc tgctcgacca gcatcgcaac caggatgcgc
     3541 tcacggtcct gaaggatttg catgacagtg gatcgaaaaa acatgttgcg gcgctgcgct
     3601 tggaactcga ggcgcaacag cgcgagaaaa actggggaca agtgctgaat ctggtcgcac
     3661 agctggaaaa gcgcggtttc ttcgatgccg gccaggcgca gcaattgcgg ctctacgcga
     3721 cgcaagagaa tctcaagcgg caggcgcata acatcgccgc gctccaagaa ttctggaaga
     3781 aaatccccac ggcggacaaa aagatcagcg ggattgccgc cactgccgcg cgctgcttta
     3841 tctcgcttgg cggctgccgc atggcgcagg aaatcatcga gcagagcctg gacgcgcaat
     3901 gggacagcga tctcatttgc ttatactcgg aatgcctggg gacggatacc ttgaggcaga
     3961 tcgagcgtgc ggagagacgg ctgtttgatc acccttatga cgtctgcctg ctgctcaccc
     4021 tgggcaagct gtgcgcccat cagagacttt ggggcaaggc gcaaagctat ctggaagcca
     4081 gcctcagcgt ggagccgacg gctaccgcgc accgtgagct cgcgaaacta cacgaaaaaa
     4141 tcggccaaag cgaagctgcg gatattcact accggaaaag cctcgatttg actgtgcggc
     4201 aattgcatga tattaccggc ggcaggcgca aggaagcgct ttagattttt tgaaccgcca
     4261 ggtctgccgg gatttaaggt caagcgctta atacttcttg gcgcacattc cttcgtcaag
     4321 ctcaagacca agcttggcgt tcagtatgcc ggtttgggat cgttggagta ggtaaaggcg
     4381 tcggagtaga actcttccaa ggagagcttg cgcatggcgg tgaaatcgcg gcgcgcggct
     4441 tccaccatca ccggcgcgcc gcaggcataa acctgaaagc tgctcaaatc cgcaaaatca
     4501 tctatcaccg cctgatgcac caggccgctg cgcccctgcc agcggtcttc gggcagtgct
     4561 tcggagagca cggggatgaa gctgaaattg tcgtgctcaa gctgccattg ctgcggcaat
     4621 tgcagcatgt atagatcggc gagcttgcgc gctccccaat acaagaccat ttcacgcttg
     4681 ctttccttat agaaagcgtg ctcgatgatg cccttgattg gcgcgaagcc ggtgccgctg
     4741 gcgacaagaa taatcggttt gtcggaatcc tcgcgcagga aaaaagtgcc gagcggccct
     4801 tcaaagcgca agatttcgcg ctctttcatc ttgttgaaca cgtaatcggt gaacgctccg
     4861 cccacgatat tgcggacgtg cagttgcaaa cactcgtcgt cgtgcggcgg gtttgccatt
     4921 gagaaactgc ggcgcttgcc ttccttcatg aggatgtcaa tgtactggcc ggcgaggaac
     4981 tgcaagcgct cgttagcggg aagcttgagg taaagcacga tcacatcgtc tgcaggccgc
     5041 tccattttct gtacacggca tggcagtttc ctgatttgaa tatccttgac cgcgttgatt
     5101 tcgcgcgctt cgataaccaa atcggtttgc ggcttggcct ggcaaaaaag cgccatgccc
     5161 gcaagttttt ccgcttcgct gagcgcgctt tcctgataga cgccgtaatt caccttgcct
     5221 tcgaggattt tgcccttgca cgaaccgcag gcgccgttgc ggcagccgta cggcaggcca
     5281 aagccttggc gcagcgctgc ttcaagcacg ccttcgccgg tctcaacgaa gaatgcgtga
     5341 tcgcttggct tgatggtaac ctgaaaagac attcaactaa cctttaagga caggcatacc
     5401 cctttgacag gggatacctt aaaatataag acatgagacg gctgttgatt atcggttgcg
     5461 gcgacgtcgc gctgcgcatg gtgcggaagc tgcccgcagg ctaccgtgtt tatgcgctct
     5521 cccactcacc cgagcgctac catctgctgc gcgagcacgg cctcacgccc attcccggca
     5581 atctcgacga gccggatacg ctggagatgc tcggcgggct tgctcatgat gtggtgcatt
     5641 ttgcgccgcc gccaagccag ggcacgaaag atacacgcac cgcacatctg cttgcaacgt
     5701 tgacaaaggg aaaaatttta ccacatcggc tcatctacat cagtaccagc ggtgtctatg
     5761 gcaactgcga cggcgatata gttcccgaaa cgcgcccttt gcggccgcac accgcgcgcg
     5821 ccaagcgccg cttggacgcc gagcgcagaa tccgcgaatg gggaaagcgc agccgagtgt
     5881 gcgtgagtat tttgcgagtg ccgggaattt atgccggcga tcgcttgccg ttggagcacg
     5941 tcaaaaacgg cactcctgca ctgttacccg agcaggatgc gtacagcaat cacattcacg
     6001 cggacgattt ggcgagcatt gttattgcgg cgttgcgttt cggcaaaccg ggaagggtgt
     6061 ataacaccag tgacgattcc gagctcaaaa tgggagatta ctttgatctg gtggcagacc
     6121 gctttgggtt gccgcggccg acacgcattt cttatagcga agcacgtcaa cgcatacccg
     6181 agaacctgtt gtcgtttatg caggaatcgc ggcggcttgc aaaccggcgc atgaagcgcg
     6241 aattgcaggt gaaattgcgc tatccggtgg tggcag
//
LOCUS       DATIOW010000050         4963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1544781, whole genome shotgun sequence.
ACCESSION   DATIOW010000050 DATIOW010000000
VERSION     DATIOW010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544781"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VLL03_01495"
     CDS             <1..203
                     /locus_tag="VLL03_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HSS46075.1"
                     /translation="GNYIVDTHDHPIIDPVWALYEQAVRRFGPVSTMIERDDNIPPLK
                     DLLAELNHARRIAEPILQKAAM"
     gene            287..1222
                     /locus_tag="VLL03_01500"
     CDS             287..1222
                     /locus_tag="VLL03_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HSS46076.1"
                     /translation="MRTLICGSIAYDTIMVFKDYFKNHILPEQIHILNVAFLVPDMRR
                     EFGGCAGNIAYSLRMLGGDPLIMATVGEDYQPYAYRLEKLKLSQKHVRQVPDTFTAQA
                     FITTDLDDNQITAFHPGAMNYSHYNHVPDADGVKLGIISPDGREGMLQHAREFHEQGI
                     PFVFDPGQGLPMFDGGELLNFINQADFVTANDYEAQLLQEKTGKKIKELEKLVKALAV
                     TLGARGSLIYAEGKRIQIPCVKAEVVADPTGCGDAYRAGLLYGIAQGMDWETTGRLAS
                     LMGALKIARRGGQNHSVTRAEIGALYRQHFGTNIW"
     gene            1238..1693
                     /locus_tag="VLL03_01505"
     CDS             1238..1693
                     /locus_tag="VLL03_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HSS46077.1"
                     /translation="MKNSFFLIILLVTYMVLGGCAQSLSGSAYSRGQARQEQNVRNGT
                     VESVRPVKIEGTKTPIGTVAGAAVGGVAGSTIGSGKGSGVAAILGAVVGGIAGSAAEE
                     GLTRKQGLEIIVKLDSGNTVAITQEADEQFRPGERVRIISGGGVSRVAH"
     gene            1966..2280
                     /locus_tag="VLL03_01510"
                     /pseudo
     CDS             1966..2280
                     /locus_tag="VLL03_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
     gene            complement(2474..3301)
                     /locus_tag="VLL03_01515"
     CDS             complement(2474..3301)
                     /locus_tag="VLL03_01515"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HSS46078.1"
                     /translation="MATYAIGDIQGCFSALQRLLDKIHFDESRDRLWFVGDLVNRGPD
                     SLATLRFVKSLGERAITVLGNHDLHLLMVAEGCAKTRKSDTLQDVLDAPDRKELLDWL
                     RHRRMMHAENSYAMVHAGLLPSWSAGKALSLAREAEEALRGENYRELVARMYGNQPAQ
                     WRDDLSGVDRLRVIINAMTRLRVCTQDGVMEFAHNGKPENTPPGFIPWYDVPGRASRD
                     TAIICGHWSALGLVLRENLLALDTGCLWGGNLTAVCLENRRVFQASCDEQQAPTGWQ"
     gene            complement(3361..3894)
                     /locus_tag="VLL03_01520"
     CDS             complement(3361..3894)
                     /locus_tag="VLL03_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSS46079.1"
                     /translation="MSDEHSSIIKTPKQLIAVVLAAFLVPVFTLIFISQLVVGSFGDV
                     SKNDPAMSPEAVAKRLKPVGEVVVADTAVGATNQSEIRFKEPVQPVPVAANVVAVNGK
                     AVFETNCAVCHVPGAANAPKFGDKAAWAPRIKTGIGSLYRSALNGKNVMPPKGGNAAL
                     TDAEVKAAVDYIVSQSK"
     gene            4005..4080
                     /locus_tag="VLL03_01525"
     tRNA            4005..4080
                     /locus_tag="VLL03_01525"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4038..4040,aa:Arg,seq:ccg)
     gene            complement(4094..4864)
                     /locus_tag="VLL03_01530"
     CDS             complement(4094..4864)
                     /locus_tag="VLL03_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSS46080.1"
                     /translation="MQLIAENLTYGYPQRVIGKGLSFSLSAGEALCVLGPNGSGKTTL
                     FRTLLGLLREKAGRILLDGRKLAEWPRAELAQALAYVPQAHEDAFAFTVEEIVLMGRT
                     AHVGLFASPSMQDREKAHAAIATLGLTHLTHNMYTRISGGERQLTLIARALAQGARLI
                     VLDEPTASLDFGNRARVLEMLSRIAVSGIGVVFSTHHPDEAFACAQRVMMLREGRVIG
                     TGKPSEVITASHLETLYGVPVEVVDLPGGRACLPVIRR"
BASE COUNT         1054 a         1427 c         1421 g         1061 t
ORIGIN      
        1 gcggcaatta catcgtggat actcatgatc atccgattat tgacccggtg tgggcgctct
       61 atgagcaagc cgtcagacgc ttcggcccgg tttcgaccat gatcgagcgc gacgataata
      121 tccctcctct caaagacctc ctcgccgaac tcaatcacgc ccggcgaatc gcagagccaa
      181 tactgcaaaa agccgcgatg taattgacgg cacggccttg gtgtgcgatc aaggtgccgt
      241 gttatcatta cccccgttta attttcctta agcccggggg gctagtgtgc gtacgctgat
      301 ttgcggctcg atcgcctacg acaccatcat ggtgttcaag gattatttca agaaccatat
      361 tctgcccgag cagattcaca ttcttaacgt cgcgtttctg gtaccggaca tgcgccgcga
      421 atttggtggc tgcgccggaa atattgctta cagcctgaga atgctaggtg gcgatccgct
      481 catcatggcc accgtgggcg aagactatca gccttatgcc taccgcttgg agaagcttaa
      541 gctctcgcaa aagcacgtgc gccaggtgcc ggacacgttt actgcgcagg ccttcattac
      601 caccgacctc gacgacaacc aaatcaccgc gtttcatcca ggcgcgatga actactcgca
      661 ttacaatcat gtgccggatg cagatggcgt gaaactgggc attatttcac ccgacggccg
      721 cgagggcatg ctgcagcatg cgcgcgaatt tcatgagcag ggcatcccgt ttgtgttcga
      781 tcccggccag ggcttgccga tgtttgacgg cggggaattg ctcaatttca tcaatcaagc
      841 cgattttgtc acggcgaacg attacgaagc gcagcttttg caggagaaga ctggcaaaaa
      901 aattaaggaa ctggaaaaac ttgtcaaagc cctggctgtc acattgggtg cacgcggttc
      961 cttgatttac gccgagggta aacgcatcca gattccatgc gttaaagcgg aagttgtggc
     1021 ggatcccacc ggctgcggtg atgcctaccg cgctggcttg ctctacggga ttgcccaggg
     1081 catggattgg gaaaccactg ggaggctcgc ttcgttgatg ggagcgctta aaattgcacg
     1141 gcggggcggc cagaaccaca gcgtaacccg cgcggagatc ggagcgttgt accgacagca
     1201 tttcgggacg aatatctggt agattcagga ggctcgaatg aaaaactcgt tttttttgat
     1261 tatcttgctg gtaacttaca tggtattggg cggctgtgcg caaagcctct cgggcagcgc
     1321 ttactcgcgc ggccaggcgc gtcaggagca aaacgtcaga aacggcacgg tggaaagcgt
     1381 gcgcccggtg aagattgaag gcaccaagac cccgatcggc accgtggcgg gtgccgcagt
     1441 tggcggtgtc gccggcagca cgataggcag cggtaaaggt agtggggtgg cggcgatact
     1501 cggcgccgtg gtgggcggga tcgcgggttc cgcggccgaa gaagggttga cgcgcaagca
     1561 aggcctggaa atcatcgtca agctcgacag cggcaacacc gttgcgatta ctcaagaggc
     1621 ggatgaacag ttcagacccg gcgagcgtgt gcgcatcatt tccggcggtg gcgtctctcg
     1681 agtcgcacac tagtaataaa tcctatccgg tggtgcttgg gcaatgcgtc cgtccgcctg
     1741 atatcttaga tgagcagccc aaccggtttc ttcacccgct gccatcgttt tgcgcggctt
     1801 attacgcatt tcctctacgc cgtgtggctc gcggctttct tttcccgttt cagcaaggca
     1861 cggcaagcgc aaacgctcaa gcgctggtcg ctaaagcttt tcgacatcct gaatatccgt
     1921 cttgcaatcc ggggcagtcc gcctgaaatc ctgccgcgtg attccatgct ggtcgccaac
     1981 cacatttcct ggctggatat ttacttgctc tatatccagt atccggcacg ttttgtcgcc
     2041 aagacggaag tgcgcgcctg gccgttgata ggatggctgt gtgaaaaggc cggcactttg
     2101 tttatcgagc gcgcgaagcg ccgcgatacc acgcgcatca atcagaatat cagccaggca
     2161 ctcatcaacg gcgattgcat tgcggttttt cccgagggca cgaccactga cggtacttac
     2221 ctgcgcgctt ttcattcttc gctgcttgag cccgcggtgc tggcgcaggc gagggtgtat
     2281 ccggtggcga tacgttttgt gaatcgggac ggtgcgatca acaccgaggc ggcgtatgtg
     2341 gacgatacga gtttgttggg ttcactgctg aaaattctag cgcagcgcga gatgggggcg
     2401 gaactgatat tcgccgaacc gattgcggcg gaagggaaaa accgcaggga gctcgcgcgc
     2461 gccgcggaag cggctattgc cagccagttg gggcttgctg ttcgtcacag gaagcctgga
     2521 atacccggcg gttttccaga cacacagcag tcagatttcc tccccacaag cagcccgtat
     2581 ccagcgccag aagattctcg cgcagaacaa gacccagcgc tgaccagtgc ccgcaaataa
     2641 ttgcggtgtc ccggctcgcg cgtccgggca cgtcgtacca cggtatgaat ccgggcggcg
     2701 tgttttcggg ttttccattg tgtgcaaatt ccatcacgcc gtcctgagtg cacacgcgca
     2761 gccgggtcat ggcgttgatg atgacgcgca gtcggtctac cccgcttaaa tcatcgcgcc
     2821 attgtgcggg ttgatttcca tacatgcgcg ccaccagctc gcgatagttt tctccacgca
     2881 atgcttcttc cgcttcgcgc gccagcgaga gcgcctttcc cgccgaccac gaaggcagca
     2941 agccggcatg caccatggcg tagctgtttt ccgcatgcat catgcgccgg tgcctgagcc
     3001 aatccagcaa ttctttgcgg tcgggtgcat ccagcacgtc ctgtaaagtg tcgctcttgc
     3061 gggtcttggc gcagccttcg gcaaccatca gcaaatgcag atcatgattg ccaagcaccg
     3121 tgatcgcgcg ttcgcccaga cttttcacga aacgcagcgt tgccagcgaa tccgggccac
     3181 ggttcaccag atcgccgaca aaccacagcc ggtcgcggga ttcgtcaaaa tgaattttgt
     3241 cgagcagccg ctgcaaggcg ctgaagcagc cctggatgtc gccgattgcg taagttgcca
     3301 tgatgagcgg aaaaaaaaga cgcagattta attctgcgcc tttcttattt gcatatgaat
     3361 ttattttgac tggctgacaa tataatccac tgcggctttg acttcggcat cggttaatgc
     3421 ggcgttgccg cctttggggg gcatgacgtt tttaccgttg agagcgctcc tgtaaaggct
     3481 gccaatgccg gtcttgatac gcggagccca agccgcttta tcgccaaact tcggcgcatt
     3541 cgctgcaccc ggcacatggc acacggcgca gttggtttca aacaccgcct tgccgttgac
     3601 agcaacgaca ttagctgcga ccggtacggg ctggacgggc tctttgaatc ttatctcgga
     3661 ttgatttgtg gctccaacgg cagtgtccgc caccaccact tcaccgaccg gcttcaagcg
     3721 tttggccacc gcctccggcg acatcgccgg atcgttttta ctcacatcgc cgaaactgcc
     3781 caccactagt tgcgaaataa aaatgagggt aaagacggga accagaaaag cggccagaac
     3841 taccgcaatt agctgcttgg gggtcttgat tatcgaagaa tgctcgtcgc tcatggaaaa
     3901 tccctgcctg aaaaagccgt aattatagcg ggaatcgtgc ggcggtcata gacgcttgct
     3961 agggaaaacg ctaaactgtg agcgtttaaa acaccaatca atacgcgccc ttagctcagt
     4021 ggatagagtg ttggcctccg aagccaaagg tcactggttc gaatccagta gggcgcgcca
     4081 attttagaag ctctcaacgc cgaatcaccg gcaggcacgc gcggccgccg ggcaaatcga
     4141 cgacttccac cggcacgcca tacaaagttt ccagatgtga agcggtaatc acctcactcg
     4201 gctttccggt tccgatcacc cggccttcgc gcagcatcat cacccgttgc gcgcaggcaa
     4261 aggcttcgtc gggatgatgc gttgaaaaca ccaccccgat gccgctaacc gcgatgcgcg
     4321 agagcatttc cagcacccgc gcccggttgc cgaagtccag gctggcggtg ggctcatcga
     4381 gcacgataag gcgcgccccc tgcgccaggg cgcgcgcaat cagcgtgagc tggcgctcgc
     4441 cgccgctgat gcgcgtgtac atgttgtgcg tcagatgcgt aagtcccagc gtcgcgatgg
     4501 ctgcatgcgc cttctcgcgg tcttgcatcg aggggctggc aaacaatccg acgtgcgcgg
     4561 tgcggcccat cagcacgatt tcttcaacgg taaaagcaaa agcgtcttcg tgagcctgcg
     4621 gcacataggc cagcgcttgc gcgagttcgg cgcgcggcca ttcagcgagt ttcctgccgt
     4681 cgagcagaat ccggcccgcc ttctcgcgca aaaggcccag cagcgtgcga aacagcgtgg
     4741 tcttgccgct gccgttgggg ccgagcacgc acagcgcttc accagcgcta agcgaaaaac
     4801 tcagcccttt gccgatgacg cgctgcgggt agccgtaagt cagattttcg gcgatgagct
     4861 gcatgcgtta atgccaaccc cggcgcgcgg ttgccaatat ccatacaaaa aatggcgtgc
     4921 cgatcaacgc cgtcagcacg ccgggaggaa tttcgatatt cgc
//
LOCUS       DATIOW010000051         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_630549, whole genome shotgun sequence.
ACCESSION   DATIOW010000051 DATIOW010000000
VERSION     DATIOW010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630549"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..>2033
                     /locus_tag="VLL03_01535"
     CDS             195..>2033
                     /locus_tag="VLL03_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46081.1"
                     /translation="MTTQHKTALKMRHGLSFEDLYRRDGLLRLDAAFLNFLSEADAAL
                     RQRLEAGRATAAALTNNEESELLIALSPHLDDFIADLLGIQEEAGKLSARHHELAPLY
                     TCKRLFVQRKAMHKVKPEEAAAVDGSAVEKQLEKLFCEPFSELVFAQHVTEWQKDEAT
                     NADKLQLALLYAAWAALTPAGRERHRRGVLFKAPSKLDYIRLVPVVADESKGYTAYSL
                     DEKHLRRRQGFALTDSGTDLVGALDEAHYCIWCHEQDKDSCSKGLKEKCVPRHGSSAE
                     SGSGAQPSGNNTVLGGTPPTVSAQSFAHRDPVGPADGFKKSPFGETLAGCPLEEKISE
                     FHKVKTQGQVIGALAMIIVDNPMVAATGHRICNDCMKSCIYQKQDPVNIPQAETRTLK
                     DVLELPWGFEIYSLLTRWNPLDLRRPYPKAPTHRRVLIAGMGPAGFTLAHHLMNDGHV
                     VVGIDGLKIEPLPGEISGVDTHGKRVPFKPILDIGELYESLDDRVMAGFGGVAEYGIT
                     VRWDKNFLKIIRLLLERRSQFAMFGGVRFGGTLTLDEAFAQGFDHITLAAGAGRPTVI
                     DMPNALARGVRTASDFLMALQLTGAAKADSIANMQLRLPVVVIGGGL"
BASE COUNT          463 a          593 c          568 g          409 t
ORIGIN      
        1 catatcacct acaaaaacct cgccgaagat gtaggcagac ctgatgtttc cccggaacag
       61 gttttggctt agcgtacgat ttgaatcttt ccaccacgca tataatccaa acatatatct
      121 atataacctc agcatggttg aatgacgtat ccttaaaagc gataatgcat gcttttaaac
      181 ggattccgcc acgcatgact acgcaacaca aaacagcgct taagatgcgt cacgggcttt
      241 ctttcgaaga tctctaccgc cgggacggct tgctgcggct cgacgccgcg tttctgaatt
      301 ttctcagcga agcagacgcc gcgctgcgcc aacgtcttga agccgggcgc gccaccgccg
      361 ctgcgctgac gaataacgaa gaatcggagc tgctgatcgc gctgagcccg cacctcgatg
      421 acttcatagc cgatttgctc ggcattcagg aagaagcggg taaactttca gcgcgtcacc
      481 atgagctcgc gccgctttac acttgtaagc gcctgttcgt ccagcgcaag gcgatgcaca
      541 aggtgaaacc tgaggaagcc gcggcagtag acggctctgc ggttgagaaa cagcttgaaa
      601 aacttttttg cgaaccgttt tcagaacttg tcttcgcgca gcacgttacc gaatggcaaa
      661 aggatgaagc aacaaatgcc gacaagctgc aactcgcact gctatatgcc gcatgggcgg
      721 cattgacacc ggcaggacgc gaacgccatc gccgtggcgt attgttcaaa gctccatcca
      781 agctagacta tatacgactc gtgccggtgg tcgcggatga aagcaaaggc tacaccgctt
      841 attccctcga cgagaaacac ttgcgccgcc gtcagggctt cgcgctcaca gattccggca
      901 ccgacctggt tggagcgctc gacgaagccc attactgtat ctggtgtcat gaacaggaca
      961 aggattcctg ctctaaaggg ctcaaggaaa agtgcgtgcc gcgacacgga agctcagccg
     1021 agagcgggag cggcgcgcag ccctccggga acaatactgt tcttggaggc actccgccca
     1081 ccgtctccgc acaaagcttc gctcaccgtg atccggtggg tcccgccgat ggattcaaaa
     1141 agagcccgtt tggcgaaacg ctcgctggct gccccctcga agaaaaaatt tccgagttcc
     1201 acaaagtgaa gacccagggg caggtcattg gagcactggc gatgatcatc gtggacaatc
     1261 cgatggtcgc ggccaccgga catcgcatct gcaacgactg catgaagtcc tgcatctacc
     1321 agaaacagga cccggtcaac atccctcaag cagagacgcg cacgctgaaa gatgtgctgg
     1381 aactgccgtg ggggtttgaa atttacagcc tgctcacccg ctggaacccg ctcgatctgc
     1441 ggcgtcccta tccgaaagcg ccgactcatc gccgcgtgct tattgcaggc atggggcctg
     1501 ccggttttac tctggcgcac catctgatga acgacggcca tgtcgtggtc ggcatcgacg
     1561 gcttgaagat cgaaccgctg cccggcgaaa tttcaggcgt ggatacacac ggcaaacgcg
     1621 tgccgttcaa gccgatcctc gacattggcg aattgtacga atcgcttgac gaccgggtga
     1681 tggcgggctt cggcggcgtg gccgaatacg gcatcaccgt gcgctgggat aagaatttcc
     1741 tcaagatcat tcgcctgctc ttggagcgcc gcagccagtt cgcgatgttt ggcggcgtgc
     1801 gctttggcgg cacgctcacg ttggacgaag cttttgcgca aggcttcgac catattacac
     1861 tggcggcagg cgccggacgc ccgactgtga tagacatgcc caacgcactt gcgcgtgggg
     1921 tgcgcaccgc ttccgatttc ctgatggcgc tgcaattgac cggcgcggca aaagcggact
     1981 cgattgccaa catgcagctc agactgccgg tggtggtaat cggcggcggc ttg
//
LOCUS       DATIOW010000052         7850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_326023, whole genome shotgun sequence.
ACCESSION   DATIOW010000052 DATIOW010000000
VERSION     DATIOW010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7850
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326023"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..133
                     /locus_tag="VLL03_01540"
     CDS             <1..133
                     /locus_tag="VLL03_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HSS46082.1"
                     /translation="IMGVMMLAANKGSSIAIETEGEDENEAMQALLNLIGNCFGESE"
     gene            225..1949
                     /gene="ptsP"
                     /locus_tag="VLL03_01545"
     CDS             225..1949
                     /gene="ptsP"
                     /locus_tag="VLL03_01545"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HSS46083.1"
                     /translation="MSFTMHGIGVSEGIAIGQAHLLSHATLEVAHYNVPEREVAEEIA
                     RFHAAIKEVQGELETLHENMAANAPAELAAFLDLHWMILSDPIISRETPKRIEESRCN
                     AEWALKQQMDELLEQFDQIEDNYLRERKADVVQVGERVLKSLLGHPGHVPPPQDREQN
                     SILVAHDLSPAEVIQFKQLHFGAFITDIGGPMSHTAIVARSLNIPSIVALHQARQLMR
                     EGDTVVVDGTQGVVIVNPDKQILVEYKLRQTQRELERQKLKKLKLTRAATIDGTPVEL
                     HANIELPEDIDGVKENGATGIGLFRSEFLFLNRNDLPSENEQYEAYRKVAMKMRGLPV
                     TIRTLDLGADKSINGADRSLTNPALGLRAIRFCLAEPQMFHTHLRAILRASSFGNIRI
                     LIPMLSNIAELNQTMQAIEQAKASLDKDRIHYNRRIEIGGMIEIPAAALALGSFIKIL
                     DFLSIGTNDLIQYTLAIDRADDAVAHLYDPLHPAVLHLVSHTIATANKNGIPIAVCGE
                     MAGDVSLTRLLLGFGLRQFSMHPAYLLTVKQQVLKTNLPEIKAVTQKILKTDEPEKVR
                     ELLSRLNS"
     gene            2285..3478
                     /locus_tag="VLL03_01550"
     CDS             2285..3478
                     /locus_tag="VLL03_01550"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010999994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HSS46084.1"
                     /translation="MKRQEMDPSISNRLFDPATADRPPAENSVGVVTPQKMHFDMPLA
                     FKCGAVLDEYDLVYETYGALNAAKSNAVLVCHALSGTHHVAGYYADDPNNIGWWDNLI
                     GPGKPLDTDRFFVIGVNNLGGCHGSTGPSSINPKTGRPYGADFPVVTVEDWVMAQARL
                     VDRLGIDCFAASIGGSLGAMQALQWTLSLPDRMRHALVIAAASKLSAQNIAFSEVARQ
                     AIMSDPDFHGGDYYAHGVIPKSGLRVARMLGHITYLSNDVMMEKFGRALSSGTYNFHF
                     DVEFEIESYLRYQGDKFASRFDANTYLLMTKALDYFDPAHDYHCDLTAALARARAGFF
                     VASFTSDWRFAPARSREIVKALLDNNLDVSYAEINAQHGHDAFLLDDPHYHALLRAYF
                     ENIKL"
     gene            3475..4107
                     /gene="metW"
                     /locus_tag="VLL03_01555"
     CDS             3475..4107
                     /gene="metW"
                     /locus_tag="VLL03_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HSS46085.1"
                     /translation="MSALELSETLLHDPALKERADLAAVAAWIRPGSKVLDLGCGDGV
                     LLRYLKQSRSVRGYGVEIDDANILACVRNGVNVIQNDLETGLSGFESNSFDYVILSQT
                     LQAMRHTESIVKEILRVGREGIVTFPNFGYWRARWQVSRGRMPVSKNLPYQWYDTPNV
                     HLCTIDDFEAFCAKHGVRILERVVMTNDSIVTLFPNLLGSLAVYRFEQKK"
     gene            complement(4183..5424)
                     /locus_tag="VLL03_01560"
     CDS             complement(4183..5424)
                     /locus_tag="VLL03_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSS46086.1"
                     /translation="MNSNPYLEIFRSRRIAVVLLFGFSSGLPLALTSSTLQAWLTVSN
                     IDIQTIAWFSWIGVPYLVKFLWSPLMDHFVPPWLGRRRGWMLATQLGLFTGIAGMGLT
                     SPNDSLLLVGCLALWVAFVSASQDIVVDAYRTDLLAPPERGMGAAVGVLGYRVAILVS
                     GGLALILADHIGWRTAYFLMALLMGVGILTSFLAPEPQTPAAAPRTLQAAVLEPLKDF
                     FSRKNALLLLLFIILYKFGDALAATLTTAFLIRGAGFTPTDVGVINKGLGLASLLLGA
                     LIGGVLLTRMSLFRALMWFGWLQAISNLSFMFLAWAGKSYGWLVFAVGFENLASGMGT
                     AAFVALIMSLCNHSFSATQYALLSALASLGRVLFGPLTGELVNLMGWSNFFFATFFAS
                     LPGLWLLWRLRREIPAIEKSN"
     gene            complement(5497..6273)
                     /locus_tag="VLL03_01565"
     CDS             complement(5497..6273)
                     /locus_tag="VLL03_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HSS46087.1"
                     /translation="MLRIITLNLNGIRSAAKKGFFEWLAKTHADVVCVQEVKAQAADM
                     TPQMLKTRNYSGYFHYAERKGYGGVGLYCRRDPDKVITGLGIADIDAEGRYLRCDFGN
                     LSVVSVYLPSGSSSEERQQIKFSFMGRFFPHLQKIVKSGREFILCGDWNIAHQEIDLK
                     NWRGNQKNSGFLPEERAWLTQVFDELGWVDVFRRLNKEPEQYTWWSNRGQAWAKNVGW
                     RIDYHIATPGIAAKAKKTAIYKERRFSDHAPLIIDYDYTL"
     gene            6275..6955
                     /gene="pyrE"
                     /locus_tag="VLL03_01570"
     CDS             6275..6955
                     /gene="pyrE"
                     /locus_tag="VLL03_01570"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HSS46088.1"
                     /translation="MKSHEGRINLSVLGQEFIKFATDRKVIRFGEFKTKAGRQSPYFF
                     NAGLFHDGASLIELAQFYAKAILSAEMPFDMLFGPAYKGIPLVASIAIALAQKGRNVP
                     FSFNRKEVKDHGEGGSVIGAPLKGRVLIVDDVISAGTSVRESVELIRLSRAEPCGVAI
                     ALDRMERGSGGLSAVQEVHKNYGLPVISIATLDDVLSYLQGREELVHNLLAVQKYRDM
                     YGVNANAV"
     gene            6945..7574
                     /locus_tag="VLL03_01575"
     CDS             6945..7574
                     /locus_tag="VLL03_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HSS46089.1"
                     /translation="MLSKFLIPKNLAWVLLLLMPGLPAFAATYKWVDENGVTHYGDSI
                     PPEYRDRANTEMNKEGVILRKNAPALTPEQLKAKEAELVKRRQEAEEKRKDSTLMRTY
                     TSVEEIDLARDRNLQQIELVIENFQTQLKTLQADLDASRKRAKAYALNDQPVPEQLQE
                     DIEYMERIKQNAEVAILQKRAEAKEIRARFEEDKKRYIELTQNAPDSKK"
     gene            complement(7588..>7850)
                     /gene="gatB"
                     /locus_tag="VLL03_01580"
     CDS             complement(7588..>7850)
                     /gene="gatB"
                     /locus_tag="VLL03_01580"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419089.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HSS46090.1"
                     /translation="AHLLGSTSTTTASITITIVDLWPRDDLAELERVVDQIIDANPSQ
                     VADYHGGKEKAFNFLVGQAMKATKGKANPAQLNEILKRKLAG"
BASE COUNT         1818 a         2342 c         2169 g         1521 t
ORIGIN      
        1 catcatgggc gtgatgatgc tggcggcgaa caaagggtct tcgattgcca tcgagaccga
       61 gggcgaagac gaaaacgaag cgatgcaggc attacttaac ttgatcggga attgctttgg
      121 ggaaagcgaa tgatgcagtg aagagtgaaa agtaaacggt aaagagtaaa aacattacgt
      181 attactgttc actgttcacc tttcactgct cacggggttt tgcgatgagc tttaccatgc
      241 atggcatagg cgtatccgaa ggcatcgcca tcggccaggc gcatctgtta tcgcatgcga
      301 cgctggaagt cgcgcattac aacgtgcccg agcgtgaagt cgccgaggaa attgcgcgct
      361 tccacgccgc catcaaggaa gtgcagggtg agttggaaac gctgcacgag aacatggcgg
      421 ccaacgctcc cgccgagctc gccgcattcc tcgacctgca ttggatgatt ctctccgacc
      481 ccatcatctc cagggaaacg ccgaaacgca tcgaagaaag ccgctgcaac gccgagtggg
      541 cgctcaagca gcaaatggac gagttgcttg agcagttcga ccagatcgaa gacaactacc
      601 tgcgcgaaag aaaagccgac gtggtccagg tcggcgagcg cgtgctgaag tcgctgctgg
      661 ggcatcccgg ccatgtcccg ccgccacagg accgcgagca gaacagcatc cttgtcgccc
      721 acgatttgag ccccgccgaa gtcatccagt tcaagcagct ccatttcggc gcgttcatca
      781 ccgacatcgg cgggccgatg tcgcacaccg cgattgtcgc gcgcagcctc aacattccct
      841 ccatcgtcgc gctgcaccag gcgcggcagc tgatgcgcga aggcgacacg gtggttgtcg
      901 acggcacgca gggcgtggtc atcgtcaatc ccgacaagca gattctcgtc gaatacaagc
      961 tgcgccagac gcagagggaa ctcgagcggc agaagctcaa gaagctcaag ctcacccgcg
     1021 ccgccaccat cgacggcacg ccggtggagc tgcacgccaa catcgaattg ccggaggaca
     1081 ttgacggggt caaggaaaac ggcgcgaccg gcatcggcct gttccgcagc gagttcctgt
     1141 ttctgaatcg caacgacctg ccgagcgaga acgagcagta cgaagcctac cgcaaggtgg
     1201 caatgaaaat gcgcggattg ccggtcacca tccgcaccct cgacctcggc gcggacaaga
     1261 gcatcaacgg cgccgatcgc agcctcacca atccggcgct gggattgcgc gccattcgtt
     1321 tctgcctcgc cgagccgcag atgttccaca cgcatttgcg cgccattttg cgcgcctcca
     1381 gcttcggcaa catccgcatc ctgattccaa tgctctccaa catcgccgaa ctcaaccaga
     1441 ctatgcaagc gattgagcag gcaaaggcga gcctcgacaa agaccgcatt cactataaca
     1501 gacgcattga aatcggcggc atgatagaaa taccggctgc ggctctggcg ttgggttcat
     1561 tcataaaaat actcgatttc ctctccatcg gcaccaacga cctgattcaa tacacgctgg
     1621 cgatagaccg cgccgacgat gcggtggccc atctctacga cccgctgcat cccgcggtgc
     1681 tgcatttggt ctcgcatacc atcgccaccg ccaacaaaaa cggcataccc atcgcggtgt
     1741 gcggagaaat ggcaggcgat gtttcgctca ctcgtctgtt gctcggcttt ggcttgcgcc
     1801 agttctcgat gcatccggcg tacctgctca ccgtcaagca gcaggtgctc aaaactaatc
     1861 ttccggaaat caaggccgtc acgcaaaaaa tactcaaaac cgacgagccg gaaaaagtac
     1921 gggagttgct gtcccgcctg aatagctaga accctatcga aaagcaaaaa gcagcccgct
     1981 cgcaactcga ggcggattgt ccgcttgaca aacgccgacc tccaaagtaa tctgcaatca
     2041 agcgccgatt tgtgccggct tgcgggcgct taataaatac agctaaaaga taaaacccgc
     2101 tcgccaaaca acagcgggtt ttttatttat gcgttgccgc gcatatcgcc gagttaaaag
     2161 acaacttgcg ggcgagggaa tctaactcat caacataggc ttgtctagag gtgagatggg
     2221 ttaccaaatg ctgctaaagc gtcgcctctt tctttccgga tcgggcaacg caggatgacc
     2281 ggaaatgaag aggcaagaaa tggatccgag tatttccaac cggctttttg atcccgccac
     2341 ggcggatcgt cctcccgccg agaattcagt cggcgttgtg acgccgcaga agatgcactt
     2401 cgacatgccg ctcgcgttca agtgcggtgc ggtgctcgat gaatacgacc tcgtttacga
     2461 aacttacggc gcgctgaatg cggcgaaatc caacgcagtg ctggtgtgcc acgcgctttc
     2521 cggcacgcac cacgtcgcgg gttactacgc cgatgacccg aacaacatcg gctggtggga
     2581 caatcttatc ggcccgggca aaccgctcga taccgacagg tttttcgtca tcggcgtaaa
     2641 caacctcggc ggctgccacg gctccaccgg cccttccagc atcaacccca aaactggcag
     2701 gccctacggc gcggatttcc cggtggtgac agtggaggat tgggtgatgg cgcaggcgcg
     2761 gctcgtcgac cgcctcggca tcgactgctt tgccgcgtcc atcggcggca gcctcggcgc
     2821 catgcaggcg ctgcaatgga cgctttccct ccccgaccgc atgcgtcatg cgctggtgat
     2881 tgccgccgca tccaagctct cggcgcagaa catcgccttc agcgaagtcg cgcgacaggc
     2941 catcatgtcc gacccggatt ttcacggcgg ggattattac gctcatggcg tcattcccaa
     3001 aagcgggcta cgcgtggcgc gcatgctcgg ccacatcact tatctttcca acgacgtgat
     3061 gatggaaaaa ttcggccggg cgctcagttc cggaacatat aatttccatt tcgatgtcga
     3121 atttgaaatt gaatcgtatc tgcgttatca gggcgacaag ttcgcctcgc gcttcgacgc
     3181 caacacttat ttgctgatga ccaaggcgct cgattacttc gacccggcgc acgattacca
     3241 ctgcgatctc accgcggcgc tcgcccgcgc cagagcggga ttttttgtcg cttcgttcac
     3301 ttcggactgg cgctttgctc ccgcgcgctc gcgggaaatt gtgaaagcgc tgctcgacaa
     3361 taatctcgac gtaagctacg ccgaaatcaa cgcgcagcac ggacacgatg cgtttctact
     3421 ggacgatccg cactaccacg cgctgctgcg cgcctacttc gagaacatca agctatgagc
     3481 gcgcttgaac tcagcgagac cctcctgcac gatccggcgc tgaaggagcg ggcggacctt
     3541 gccgccgtcg ccgcctggat caggcccggc tcgaaagtgc tggacctcgg ctgcggcgac
     3601 ggcgtgttgc ttcgctatct caaacaatcg cgctcggtgc gcggctacgg cgtggaaatt
     3661 gacgacgcca acattctcgc ctgcgtcagg aatggcgtga acgtgattca gaatgacctt
     3721 gaaaccgggc tttccggctt cgagtcaaac tcgtttgatt acgtgattct gtcgcagacg
     3781 ctgcaggcga tgcgccacac cgagagcatc gtcaaggaaa ttctgcgcgt ggggcgcgaa
     3841 ggcatcgtca ccttccctaa cttcggctac tggcgcgcgc gctggcaggt gtcccgcggc
     3901 cgcatgccgg tgtcaaaaaa tctcccctac cagtggtacg atacgcccaa cgtgcatctg
     3961 tgcaccatcg acgatttcga ggcattctgc gcgaagcatg gcgtgcgcat actcgagcgg
     4021 gtggtgatga ccaatgacag tatcgtcacg ctatttccca atctgctggg cagcctggct
     4081 gtgtaccgct tcgaacagaa aaaataaatc cgggcgttcg tgatgagctt atcgaatcat
     4141 aaacgccctg ttcgctctgc gacgggttcg gcgcgaacgg cgctaattgc ttttctcaat
     4201 cgccggaatc tcccggcgaa gccgccaaag taaccacagg cccggcagtg aggcaaaaaa
     4261 agtcgcgaag aagaaattcg accagcccat caggttcacc aattctccgg taagcgggcc
     4321 gaacaagacg cgtcccagcg aggccagcgc ggaaagtagc gcgtattgcg tcgcgctgaa
     4381 gctgtgattg cacaggctca tgatgagcgc gacaaacgcc gccgtgccca tgccgctggc
     4441 gagattctca aaccccactg cgaacaccag ccagccatag cttttccccg cccaggcgag
     4501 gaacatgaag gagagattgg aaatcgcctg cagccagccg aaccacatca gcgcccggaa
     4561 caagctcatg cgcgtgagca acacgccgcc aatgagcgcg cccagcaata gtgacgccaa
     4621 ccccagcccc ttgttaatca cccccacgtc ggtcggcgtg aagcccgctc cccgaatcaa
     4681 aaacgcggtg gtgagggtcg ccgccagcgc atcgccgaac ttgtagagga tgatgaagag
     4741 caagaggagc agcgcgtttt tgcgcgaaaa aaaatccttg agcggctcga gcaccgccgc
     4801 ctgcaaggtg cgcggcgcgg cagcaggcgt ttgcggctcg ggcgcaagga aagaagtgag
     4861 tattcccacg cccatcagga gcgccattaa aaaataagca gttctccagc cgatgtggtc
     4921 ggcaagaatc agcgccagcc cgcctgaaac cagaatcgca acgcgatagc ccagcacgcc
     4981 gaccgctgcg cccatgccgc gctcgggcgg cgccaacaaa tccgtgcggt acgcgtccac
     5041 cacaatatcc tgggaagcgg aaacgaatgc gacccaaagc gctaagcagc ccaccagcag
     5101 cagtgaatcg ttgggcgaag tgagccccat gccggcgatg ccggtgaaca aaccaagctg
     5161 agtcgccagc atccagccgc gccgccgtcc cagccatggc ggcacgaagt ggtccatcag
     5221 cggcgaccag agaaatttca ccagatacgg tacgccgatc caggaaaacc aggcaatggt
     5281 ctggatgtcg atattggaaa ctgtgagcca ggcctgcagc gtgctgctgg tcagcgcgag
     5341 cggcagtccc gacgaaaaac cgaacagcag caccaccgcg atgcgccggc tgcggaaaat
     5401 ttcaagatag ggattcgaat tcattcttgt tattttcacc ggggaggcgc agagaaaaag
     5461 cgcaacttga atccttcgag tcccggtgcc gtcaagtcat aaggtgtaat catagtctat
     5521 gatgagcggc gcatggtcgg agaatcgtcg ttccttatat atcgccgtct tttttgcctt
     5581 ggcggcgatg cccggcgtgg cgatgtgata atcgatacgc cacccgacgt tcttggccca
     5641 ggcctggccg cggttcgacc accaggtgta ctgctcaggt tccttgttca aacgcctgaa
     5701 tacatccacc cagcccagct cgtcgaacac ctgcgtgagc cacgcccgct cctcgggcag
     5761 gaagccggaa ttcttctggt tgccgcgcca gtttttcaag tcgatttcct ggtgcgcgat
     5821 attccagtcg ccgcacagaa tgaattccct gccgcttttc acgatctttt gcaggtgcgg
     5881 aaaaaatctt cccatgaagc tgaacttgat ttgctggcgt tcctccgagc tggagccgga
     5941 aggcagataa accgaaacca cgctcaggtt tccgaaatca cagcgcaggt agcgcccctc
     6001 ggcgtcgatg tcggcaatgc ccagccccgt aatcaccttg tccggatcgc gcctgcaata
     6061 aagccccacg ccgccgtagc cttttctctc tgcgtagtgg aagtatccac tgtaatttcg
     6121 tgttttaagc atttgcggtg tcatgtctgc ggcttgcgct tttacttcct gcacacagac
     6181 gacatcggcg tgcgttttcg ccagccattc gaaaaaaccc tttttcgccg ccgagcggat
     6241 gccgttgagg ttaagagtta taatacgcag cactatgaaa tctcatgaag ggcggattaa
     6301 tttgtcggtc ttgggacagg aattcataaa attcgcgacc gaccgcaagg tcatccgctt
     6361 cggcgaattc aagaccaagg ctggccgcca gtcgccgtat tttttcaacg ccggtctgtt
     6421 tcacgacggc gcgtccctga ttgagcttgc gcaattctac gccaaagcca ttctctccgc
     6481 tgaaatgccc ttcgacatgc tgttcggccc ggcttacaag ggcattccgt tggtggcaag
     6541 cattgccatc gccctggctc aaaaagggcg caatgtgccg ttcagcttca accgcaagga
     6601 agtcaaggat cacggcgaag gcggctctgt catcggcgct ccgcttaaag ggcgggtgct
     6661 aatcgtggac gatgtgatct ccgccggcac ctccgtgcgt gaatcggtgg aactgattcg
     6721 cttgagccgc gcggaaccct gtggcgtagc gattgcgctc gaccgcatgg aacgcggcag
     6781 cggcgggctt tctgcggtgc aggaagttca caaaaactat gggctgccgg tcatcagcat
     6841 cgccaccctg gacgatgtat taagctatct acaaggccgg gaagaattgg tacataattt
     6901 gcttgcggtt cagaaatacc gcgacatgta cggtgtaaac gcaaatgctg tctaagttcc
     6961 tcatccccaa aaacctagcc tgggttttgc tacttctcat gccgggcctg cctgcttttg
     7021 ccgcaactta caagtgggtg gatgagaatg gcgtcaccca ttacggcgac agtatccccc
     7081 cggaataccg cgaccgcgcc aacaccgaga tgaacaagga gggcgtgatc ctcagaaaga
     7141 atgctccggc gctaacaccc gagcagctca aggccaaaga ggcggaactg gtaaaacgca
     7201 ggcaggaggc ggaagaaaaa cgcaaggact caaccttgat gagaacctat accagcgtcg
     7261 aggaaattga tctcgcgcgc gaccgcaatc tgcagcaaat cgagctggtc atcgaaaact
     7321 tccagacaca gctaaagacg ctgcaggccg acctggatgc aagccgcaag cgggccaaag
     7381 cttatgccct aaacgaccaa ccggtgcccg agcagttgca agaggacatc gagtacatgg
     7441 aaagaatcaa gcagaacgcg gaagtggcca ttctgcaaaa acgcgcggaa gcgaaagaaa
     7501 tccgcgcgcg ctttgaggaa gacaagaagc gctacatcga gctgactcag aacgcgcccg
     7561 actcaaagaa gtaactttac tccagcttta gcccgccagc ttgcgcttga ggatctcgtt
     7621 gagctgcgcc ggattggcct tgcccttggt cgccttcatc gcctgaccaa ctaagaaatt
     7681 gaaagccttc tccttgcccc cgtgataatc ggcaacctgt gacgggtttg cgtcgatgat
     7741 ttggtcgacg acgcgctcga gttctgcgag gtcgtctctc ggccacaaat ctacgatcgt
     7801 tatagttata gatgcagtgg tagtcgacgt cgagcccaac aaatgtgcgt
//
LOCUS       DATIOW010000053         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_427534, whole genome shotgun sequence.
ACCESSION   DATIOW010000053 DATIOW010000000
VERSION     DATIOW010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427534"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /gene="acnA"
                     /locus_tag="VLL03_01585"
     CDS             <1..679
                     /gene="acnA"
                     /locus_tag="VLL03_01585"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399920.1"
                     /note="catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HSS46091.1"
                     /translation="ISPAGSIKETSPAGKYLIGNGVIKADFNSYGARRGNHDVMMRGT
                     FANVRIKNLMVPPKADGTRIEGGITLHQPSGEQMSIYDAAMKYITQGTPTVVFGGEEY
                     GTGSSRDWAAKGTQLLGVKAVIARSFERIHRSNLVGMGVLPCQFKGDDSVQTLAIHGD
                     DEFDILGIEGEIRPQQDVMLVIKRKDGSKQEVKVLLRIETPIEVDYYRHGGILPYVLR
                     ELVSQAA"
     gene            complement(740..1945)
                     /locus_tag="VLL03_01590"
     CDS             complement(740..1945)
                     /locus_tag="VLL03_01590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSS46092.1"
                     /translation="MDSAAGTLRGDAKVIGLIGSAHFVSHFFQLALPPLFPMLKNEFG
                     VSYATLGAVMTVFYSFSGVFQALAGFAVDRFGARAVLLSGMGLLAASCLLAGLAPVFW
                     LLFPVMALAGIGNSVFHPADFAILNASVTPPRLGHAYSAHGIGGNLGWAAAPVMSFAL
                     GSVYGWRVALIVMGAMGLAVLAFLLAQRAALVCHHHKVEAAQGGLTRANIVLLLRAPI
                     LMCFAYFCFWSMATVGMQTFSVTALTAVYHVPLALATTALTAFLLGTSAGILAGGFVA
                     AQTSRHSLVAAGGMLLSAFFAFVLADATAPSVMALTTLMGFFLGMTVPSRDLLVRAAT
                     PAGAVGRVYGFVYSGLDLGASIAPLFFGWMLDGGHGTGIFFGIAGCMLLTMLTAVQMP
                     RFARASASA"
     gene            2064..>2372
                     /gene="msrB"
                     /locus_tag="VLL03_01595"
     CDS             2064..>2372
                     /gene="msrB"
                     /locus_tag="VLL03_01595"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HSS46093.1"
                     /translation="MKDKTVKSEREWKEQLTPEQYEVTRKKGTEPAFTGQYWNCKEKG
                     VYHCVCCGAELFDSATKFDSGSGWPSFWAPLQSENVKTEKDNSLFMRRTEVLCSACDA
                     H"
BASE COUNT          621 a          707 c          673 g          371 t
ORIGIN      
        1 catcagcccg gcgggctcaa tcaaggaaac ctcgcctgcg ggtaagtacc tcattggcaa
       61 cggcgtcatc aaggccgatt tcaacagcta cggcgcgcgg cgcggcaatc acgacgtgat
      121 gatgcgcggt accttcgcca acgtgcgcat taaaaacctg atggtgccgc cgaaagcgga
      181 cggcacacgg atcgaaggcg gcatcacact gcaccagcct tcaggcgaac agatgagcat
      241 ttacgacgcg gcgatgaaat acatcaccca aggcacgccc accgttgttt ttggcggcga
      301 ggaatacggc accggctcct cgcgcgactg ggcggccaaa ggcacccagc tgcttggcgt
      361 gaaagccgtg attgcgagaa gcttcgagcg cattcaccgc tccaacctgg tcggcatggg
      421 tgtgctgccc tgccagttca agggtgatga cagtgtgcag acgctggcta tccacggcga
      481 cgatgagttc gacattctgg gtatcgaggg cgagatcagg ccgcaacagg atgttatgct
      541 ggtgattaag cgcaaagatg gcagcaagca ggaagtaaaa gtgctgctgc gcatcgaaac
      601 accgatcgag gtggattact accggcacgg cggaattctg ccctacgtgc tgcgtgaatt
      661 agtgagccag gctgcttgat ctgacgctgt caattgaaaa gtcggcatcc ataaagcacc
      721 gacttttttt atttgcggct cacgccgaag cgctcgcgcg tgcaaacctg ggcatttgca
      781 ctgcggtgag catggtaagc agcatgcagc cggcaatgcc gaaaaagatt cctgtcccat
      841 gccccccgtc gagcatccaa ccgaaaaaca gcggcgcgat agatgcgccg agatccaggc
      901 cggaataaac gaagccatag acacggccca cggcgccggc aggggtggcg gcccgcacca
      961 gcaagtcgcg cgagggcacg gtcatgccga gaaaaaagcc catcaacgtg gtgagcgcca
     1021 tcacagaagg cgctgttgcg tcagccaaaa caaaggcaaa aaatgcactg agcagcatcc
     1081 ctcccgccgc aaccagacta tggcggctag tttgagcagc gacaaaacct cccgccagta
     1141 ttccggccga agttccaagg agaaaagcgg tgagcgccgt agtggccaga gccagtggca
     1201 cgtgataaac ggcggtgagc gccgtgaccg agaaagtctg catgcccacc gtggccattg
     1261 accagaagca gaaataagca aaacacatga gaatgggcgc ccgaagaagc aaaacgatat
     1321 tggcgcgggt aagaccgccc tgcgcagcct ccaccttatg gtgatggcag accagcgcag
     1381 cacgctgcgc caggagaaat gccaagacgg ctaatcccat cgcgcccatg acaatcaaag
     1441 ccacgcgcca gccataaaca ctgccgagcg cgaaactcat caccggcgcg gccgcccagc
     1501 cgagattgcc gccgataccg tgcgcgctgt aggcatgtcc cagacgcggc ggggttacag
     1561 aggcattcag aatggcaaaa tcggctggat gaaatacgct gttgccgatc cccgccagcg
     1621 ccatgacggg aaacagcagc cagaacaccg gcgcaagccc ggccagcagg catgaagccg
     1681 caagcaggcc catgccgctt aacagcaccg ccctcgcccc gaagcggtca accgcgaaac
     1741 ccgccagcgc ctggaatacc ccggagaagg aatagaaaac cgtcatcacg gcgcccagcg
     1801 tggcataact cacgccgaac tcatttttaa gcatggggaa tagcggcggc agcgcaagct
     1861 gaaaaaaatg gctgacgaaa tgcgccgagc ctatcaggcc gatgactttg gcgtcgcctc
     1921 ttaatgttcc ggcggcagaa tccattgcgc tccttaaatt gcataaacgc cgcgcatttt
     1981 accatgcaag tggacacccc cccatgaact tatgttatat attaggaact aacacgccac
     2041 acccaacaaa gggacatcac accgtgaaag acaaaaccgt taaatccgag cgggaatgga
     2101 aagagcagct tacgcccgaa caatacgagg tcacccgcaa gaaaggcacg gagcccgcct
     2161 tcaccgggca atactggaac tgcaaggaaa aaggcgttta ccattgcgtc tgctgcggcg
     2221 ccgaactctt cgactccgcg accaagttcg actccggcag cggatggccg agcttctggg
     2281 ccccgctgca atctgaaaat gttaaaaccg aaaaggacaa cagccttttc atgcgccgca
     2341 ctgaagtact gtgcagcgcc tgtgacgcgc at
//
LOCUS       DATIOW010000054         8285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_631444, whole genome shotgun sequence.
ACCESSION   DATIOW010000054 DATIOW010000000
VERSION     DATIOW010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8285
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631444"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VLL03_01600"
     CDS             complement(<1..205)
                     /locus_tag="VLL03_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004555699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HSS46094.1"
                     /translation="MADYKKTLNLLDTPFPMRGDLPRREPLLLKRWQEQDLYRKIREA
                     SRGRPKFVLHDGPPYANGDIHIGH"
     gene            complement(214..1251)
                     /locus_tag="VLL03_01605"
     CDS             complement(214..1251)
                     /locus_tag="VLL03_01605"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HSS46095.1"
                     /translation="MFISHGIPDQASAPVALTIGNFDGVHLGHCAMLKRLTETARARA
                     LTSCVLTFEPHPHEFFTPESAPARLTNLREKLEWFAALGVEHTHICRFDRGFAQLSAG
                     EFIGRVICRALDAKWLLVGDDFRFGKNRTGDFNLLQSAGKKYGFEVVAMPSVIVEGMR
                     VSSTLVRDALARGDLEQAAQWLGRPYSISGRVVHGDKTGRKIGFPTANIRIKHARPAL
                     SGIFAVELAGIGERALRGVASLGVRPTVKENDTPLLEVYIFDFDREIYGNHVRVDFLH
                     KLRNEEKYSDLETLRAQIGRDVENAKFFFNRGGRGGDLENSNGKPDRTYPALASDNQE
                     DFPLRPLRPPR"
     gene            complement(1348..2115)
                     /gene="cobA"
                     /locus_tag="VLL03_01610"
     CDS             complement(1348..2115)
                     /gene="cobA"
                     /locus_tag="VLL03_01610"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HSS46096.1"
                     /translation="MSGKVYLIGAGPGAPALITVRGAEILKRADIVFYDALVHPDTLA
                     FAPRARKVAVGKRCSRVSTDQRFINRNLVEAAHKYQTVVRLKGGDPMLFGRAQEEIAA
                     LRKAGIEYEIVPGVTAALGASAQVGVSLTRRGLSRSVVFVTPRVGEGEAEADWVKSVL
                     AADTAVLYMAASQAAQISVMLIAEGMPAATPVVVVEKATLPETRQIRTTLAGLSEAGL
                     WNLEGPALIFLGEVFHEVAASAVVDELMSTVKTAFGG"
     gene            complement(2112..3398)
                     /gene="cysN"
                     /locus_tag="VLL03_01615"
     CDS             complement(2112..3398)
                     /gene="cysN"
                     /locus_tag="VLL03_01615"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HSS46097.1"
                     /translation="MSAIEKIDFQDIELSRFITAGSVDDGKSTLIGRLLYDSKSIFED
                     QLTAIEKTTRRKGLEGMDLSLLTDGLQAEREQGITIDVAYRYFATPRRKFIIADTPGH
                     EQYTRNMVTGASTANLAVILIDARKGVLTQSRRHAYIASLVGIPHLVVAVNKMDLVGY
                     SQDVFNAIKRDFSAFASRLNLHDVRYIPISALCGDMVVERGDNLSWYNGTPLLELLET
                     IEIASDINTRDFRFPVQWVCRPADPAYQDFRGYMGRIESGEIQVGDSVTVLPSGLTSK
                     IKEIASYNVKHELAFAPQSVALRIEDHIDISRGDMLVKSDNLPQTTKEFGAMLCWLSE
                     QPLDVHRKYFVKHTTKTVKGLVSQLDYRVDVNTLERVGGITELRMNEIGHARLKVQQP
                     LMWDAYDRNRATGSFILIDEATHNTVAAGMVIEAVQ"
     gene            complement(3398..4351)
                     /gene="cysD"
                     /locus_tag="VLL03_01620"
     CDS             complement(3398..4351)
                     /gene="cysD"
                     /locus_tag="VLL03_01620"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HSS46098.1"
                     /translation="MSEQAALRKRKLSGAEERLHHLSHLEVLESESIHILREVAAECK
                     NPALLFSGGKDSIVLLRLAEKAFRPAHFPFPLLHIDTGHNFPEVIAFRDYRVEQLSER
                     LIVGSVEESIRRGRVVLESETQSRNVSQSVTLLDTIAEHGFDACIGGARRDEEKARAK
                     ERIFSFRDEFGQWDPKNQRPELWDLYNTRVHPGEHIRVFPISNWTELDVWQYILQERL
                     EIPVIYRAHQRAVIRRGSGLLPVTHLVQPQPGEKTENIMVRFRTVGDMTCTCPVESTA
                     VTLEQIIAETAATRITERGATRMDDQTSEASMELRKKEGYF"
     gene            complement(4348..5073)
                     /locus_tag="VLL03_01625"
     CDS             complement(4348..5073)
                     /locus_tag="VLL03_01625"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HSS46099.1"
                     /translation="MLTHPKLSTEARAVLERKTEAVKTLLTRIEKDHAPATFASSFGV
                     EDMVLLDLIARFTPGIETFTLDTGRLPEETYALMDKIAERYGVEIRIYFPEARAVEEY
                     VQRHGPNAFYESVELRKACCHMRKVEPLKRALAGKKAWVTGMRRGQSVTRGATPESEF
                     DKDHGLHKFNPLYDWTEKEVWAYVRDHNVPYNALHDKGYASIGCAPCTRAITPGEDIR
                     AGRWWWEDAANKECGLHRKSEAA"
     gene            complement(5076..7178)
                     /locus_tag="VLL03_01630"
     CDS             complement(5076..7178)
                     /locus_tag="VLL03_01630"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HSS46100.1"
                     /translation="MAFHNLADREEIEAYARAVDDFLNLQLDAERFTATRLQMGVYGQ
                     RQEGVHMVRIKIPGGRMHAHQLSTIADLLEKYSEHDVVHITTRQDIQMHYVPTRHTPT
                     VLRTLADAGLTTREACGNTIRNVTACPMAGICPREHTDVTKHLDGAVQHFLRNPLNQQ
                     LPRKFKISFSGCEADCAQGMLHCMGIVAVKKEDRFGFKVLAGGGLGHKPHEAIVVEEF
                     IEEKDLLHTMEAIVTLHNKYSDRVKRAKSRIKFLVDRFGREGFLEKYREELARTREAL
                     ANQPYPRGEWKTGGEGEICGQGAPRHTVPQKQPGLFVLPLGVPIGNLNATQLRGLAHA
                     LVEHGLDEIRTTQDQNLMVVNVPEAKLNSLRQAIAALGLREPEAGDNVVACPGTSTCR
                     LGITSSTLLGPKLRNAVGGDLRIRVSGCHNGCAQPEMGDIGIYGEGRRMHGKLIPHYQ
                     TYFGGNGLAGGGLAFKGPSVPAARIEKALERVRLTFEQDHLPNESFFAWTRRRGVPFF
                     TELLADLVQVPETEVGSVLRDHGDNQDFKVLQLGGGECAGASQMLVGSNFFEAAHERG
                     YRNALVFQRKYGEAVKCAEAIARLIGQGVVHLLGGNNKQESLADLGAELVGLLSDQPE
                     LGEQLTEFGAAFARDPEEWTDKELNPLFARLDGWTMAIAELCVSRDRQLDLSGTLPAP
                     LAAQQAVPVARPALHAAA"
     gene            complement(7205..7978)
                     /locus_tag="VLL03_01635"
     CDS             complement(7205..7978)
                     /locus_tag="VLL03_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HSS46101.1"
                     /translation="MDFLYTISGFVVGFIVGLTGVGGGSLMTPLLVLLFGIVPATAVG
                     TDLLYAAITKMGGVWVHGRRSNVDWKIVGLLALGSLPASLITVWALKSFAVEKAHFNS
                     LITTTLGVALILTALVLIFRERLQKFVKHHVGELWHERHPLPATIVTGLVLGVLVTIS
                     SVGAGALGVAALTFLYPRLPAVKIVGTDIAHAVPLTAVAGLGHMALGTVDLTLLGSLL
                     LGSLPGIYLGSHMSVRIPERVLRQILASMLVLIGGRLVW"
     gene            8116..>8285
                     /locus_tag="VLL03_01640"
     CDS             8116..>8285
                     /locus_tag="VLL03_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HSS46102.1"
                     /translation="MKLQQLRYLCEVVRQGLNVSEAAEKLHTSQPGISKQIRLLEEEL
                     GVQIFVRNGKRVV"
BASE COUNT         1678 a         2555 c         2323 g         1729 t
ORIGIN      
        1 catgcccgat gtgaatgtcg ccgttggcgt acggcggccc gtcatgcagg acgaattttg
       61 ggcggccccg cgacgcctcg cggattttcc ggtagaggtc ttgctcctgc cagcgcttca
      121 gcagcagtgg ttcgcgccta ggcaggtcgc cgcgcatcgg aaacggcgta tcaaggaggt
      181 ttagtgtctt tttgtaatca gccataaaat cttttaccgc ggggggcgca gaggacgcag
      241 aggaaaatct tcttggttgt cactagccag agccggataa gttcggtctg gttttccatt
      301 agaattttct agatctcctc cgcgtcctcc gcggttaaaa aagaattttg cattttccac
      361 gtcgcggccg atttgcgccc ttagcgtctc gaggtccgaa tatttttcct cgttacgcaa
      421 tttatgcagg aaatccacgc gcacgtgatt tccataaatc tcacggtcaa aatcgaaaat
      481 ataaacttcc agcagcggcg tgtcgttctc ttttaccgtg ggccgcacgc cgagactcgc
      541 cacgcctctc agtgcccgct cgccgatgcc ggcaagctcc actgcaaaaa tcccgctgag
      601 cgccgggcgc gcatgtttaa tgcgaatgtt ggcggtggga aaaccgattt tgcggccggt
      661 cttgtcgccg tgcaccacac ggccactgat gctgtagggc cgccccagcc attgcgccgc
      721 ttgctcaaga tcaccccgtg ccagcgcatc gcgcaccagc gtgctcgaaa cccgcattcc
      781 ttccacaatg acgctgggca tcgccaccac ctcaaaacca tatttctttc cggccgactg
      841 cagcagatta aagtcgccag tgcggttttt gccaaagcgg aaatcatcac cgaccagcag
      901 ccacttcgca tctagcgcac ggcaaatcac gcgcccgata aattctccag cgcttaattg
      961 cgcgaaacca cggtcaaagc ggcagatgtg ggtatgttcc acacccaacg ccgcaaacca
     1021 ttccagcttt tcgcgcaggt tggtgagccg cgccggcgcc gattccggtg tgaagaattc
     1081 gtgcggatgc ggctcgaagg tcagtacaca tgaagtcaat gccctggcgc gcgccgtttc
     1141 ggtgaggcgc ttgagcatcg cgcaatggcc gagatgcaca ccgtcgaaat tgccaatggt
     1201 caaggccacc ggcgctgacg cttggtcggg aattccgtgg gaaataaaca taaagcttga
     1261 attttacagt gcatccaagc cagcggctac tgcgatcgtt tgtaaagaat ttggaaagcc
     1321 gggcggatcc gcccgcagga acaacaatca gccgccgaaa gcggttttca ccgtactcat
     1381 gagttcatca accaccgcgc tcgcggccac ttcatgaaac acttcgccaa gaaaaatcaa
     1441 cgccggcccc tcaagattcc atagaccggc ttcgctcaag ccggcaagcg tggtgcggat
     1501 ttgtctcgtt tcgggaagcg tggctttttc caccactacc acaggcgttg ccgccggcat
     1561 tccctcggca ataagcatca cggaaatttg cgccgcctgg cttgccgcca tgtaaagcac
     1621 cgcggtatcc gcagcgagca cgctcttcac ccagtcggct tccgcctcac cctcgcccac
     1681 gcgcggcgtg acgaacacca cactgcggct taatccccgg cgcgtgagcg agacgcctac
     1741 ttgcgcgctt gcacccagcg ccgcggtgac accgggcacg atttcgtatt caatcccagc
     1801 tttcctgagc gcggcgattt cttcctgcgc acgcccaaac agcatcgggt cgccaccctt
     1861 gagccgcacc acggtctgat atttatgcgc cgcttccacc agattgcggt tgatgaagcg
     1921 ctggtcggta gaaacgcgcg agcagcgctt ccctacggcc acttttctgg cgcgaggcgc
     1981 gaacgctaaa gtgtccggat gcaccagggc gtcataaaac acgatgtcgg cgcgcttgag
     2041 aatctcggcg ccgcgcacag tgatcaaagc cggcgcgccg ggaccagcgc caatcaaata
     2101 cactttccca ctcattggac tgcctcgata accattcccg ccgcaacggt gttatgcgtg
     2161 gcttcatcga tcaggataaa gctgccggtg gcgcgattcc tgtcatacgc gtcccacatc
     2221 agcggctgct gcactttcaa cctggcgtgc ccgatttcgt tcatcctgag ttccgttatt
     2281 ccgccaaccc gctcgagggt attcacgtcc acccgataat ccagttgcga aaccaagccc
     2341 ttcaccgtct tggtggtgtg cttgacgaaa tatttgcggt gcacatccaa cggctgctcg
     2401 gagagccagc acagcatggc gccgaattcc ttggtagtct gcggcaggtt gtcgcttttc
     2461 accagcatgt cgccgcgcga aatgtcgatg tgatcttcga tccggagagc gacagattgc
     2521 ggcgcaaatg ccagctcgtg cttgacattg tagctggcaa tttcttttat tttcgaagtc
     2581 agcccggaag gcagcaccgt gacactatcg ccgacctgaa tctcaccgga ttcaatgcgc
     2641 cccatgtagc cgcggaaatc ctggtatgcc gggtccgctg gacggcatac ccactgtacc
     2701 gggaagcgga aatcgcgggt attgatgtcg cttgcaatct cgatggtttc cagcaattca
     2761 agcagcggcg tgccgttgta ccatgacagg ttatcgcccc gctccaccac catgtcgcca
     2821 cacagggcgg aaatcggaat ataacgcaca tcgtgcaggt tgaggcggct cgcaaaagcg
     2881 gaaaaatccc gcttgatggc gttgaacacg tcctgcgaat aacccaccag gtccattttg
     2941 ttcaccgcca ccaccagatg cgggatgccg accagcgatg cgatgtaggc gtggcggcgc
     3001 gactgggtaa gcacgccctt gcgcgcatca atcagaatca ccgccagatt cgcggtgctg
     3061 gcgccggtca ccatgttgcg cgtgtactgc tcgtggccgg gcgtgtcagc gataatgaac
     3121 ttgcgcctgg gcgtggcgaa atagcggtag gcgacgtcaa tcgtgatgcc ctgttcgcgc
     3181 tcggcctgca ggccatcggt aagcagcgac agatccatac cttccagccc tttgcgccgg
     3241 gtagtttttt caatcgccgt gagctggtct tcgaaaatgg atttggaatc gtaaagcaac
     3301 cggccgatca gcgtgctctt gccgtcatcc acgctgccgg cagtgatgaa gcgcgacagc
     3361 tcgatgtcct ggaaatcaat tttttcaatg gccgacatca gaaataacct tctttctttc
     3421 tgagctccat cgaggcttcg gaggtctggt catccatgcg cgtggcgccg cgctcggtaa
     3481 tccgcgtcgc cgcagtctcg gcgatgattt gctccagcgt cactgcggtg gattccaccg
     3541 ggcaagtgca ggtcatgtcg cccacggtac ggaagcgcac catgatgttt tccgttttct
     3601 cacccggctg cggctgcacc aagtgcgtga ccggcagcag gccgctgccg cggcggatca
     3661 ccgcccgctg gtgagctcgg taaattaccg ggatttccag ccgttcctgc aaaatgtact
     3721 gccacacatc gagttcagtc cagttgctga tggggaacac ccgtatatgc tcgccggggt
     3781 gaaccctagt gttatagaga tcccacagct cagggcgctg gtttttcgga tcccattgcc
     3841 cgaattcgtc gcggaaggaa aaaatccgtt ctttcgcgcg cgctttttcc tcgtcgcgcc
     3901 gcgcgccgcc gatgcaggca tcaaacccat gctcggcgat ggtgtcgagc aaggtcaccg
     3961 actgggagac attgcggctt tgggtttcgc tttccagcac cacccgtccc ctgcgaatcg
     4021 attcctcgac cgagcccacg atcagccgtt cgctcagctg ctccacgcga tagtcgcgga
     4081 aggcaatcac ctcggggaaa ttgtgccccg tgtcgatgtg cagcaacggg aaggggaagt
     4141 gcgcggggcg gaaggctttc tccgcgaggc gcagcagcac gatggaatcc ttgccgccgg
     4201 agaaaagcag cgccggattt ttgcattccg ccgcgacttc gcgcaggatg tggatggatt
     4261 cgctttccag cacctcaaga tggctgagat gatgcaagcg ctcctccgca ccggaaagtt
     4321 tccgtttgcg caatgccgcc tgctcgctca tgccgcctcg cttttgcggt gcaacccgca
     4381 ttccttgttt gcggcatcct cccaccacca gcggccggcg cggatgtcct cgcctggcgt
     4441 aatcgccctg gtgcaaggcg cgcagccaat gctagcgtaa cccttgtcgt gcagcgcgtt
     4501 gtagggaacg ttgtggtcac ggacataagc ccacacttcc ttttcggtcc agtcgtaaag
     4561 cgggttgaat ttatgcaacc cgtgatcctt gtcgaattcg gattcgggag tggcgccgcg
     4621 cgttaccgat tgcccgcggc gcattccggt gacccaggct ttcttgccgg caagcgcgcg
     4681 cttgagtggt tccaccttgc gcatatggca gcaggccttg cgcagttcca cgctttcata
     4741 aaacgcgttc ggcccgtgcc gctgcacgta ttcttcgacc gcccgcgcct cgggaaaata
     4801 aatgcggatc tcgacgccgt agcgttcggc gattttatcc atgagcgcat aagtctcttc
     4861 aggcaaacgg ccggtatcca gggtgaaggt ttcgattcca ggggtaaaac gcgcgatgag
     4921 atcaagcagc accatgtcct ccacgccgaa actgctggcg aacgtggcgg gcgcgtggtc
     4981 tttctcgatg cgcgtgagca gcgtcttgac tgcttcggtt ttgcgctcca gaaccgctct
     5041 cgcttcggtg gagagcttgg gatgagtaag catggtcagg ccgctgcgtg tagcgctggc
     5101 cgcgcgacgg gaaccgcttg ctgcgccgcc agtggcgcag gcaacgtgcc ggagaggtca
     5161 agctggcggt cgcgggacac gcagagttcg gcaatagcca tggtccagcc gtccagcctg
     5221 gcgaacaaag gattaagttc cttgtcggtc cactcctcgg gatcgcgtgc gaacgcagca
     5281 ccaaactccg tcagttgctc gccaagttcg ggctggtccg acaacaggcc gacaagttca
     5341 gcgccgagat cggcaaggct ctcctgcttg ttgttgccgc ccagcagatg gaccacgccc
     5401 tgcccaatca agcgggcaat cgcttcggcg catttcaccg cttccccgta cttgcgctgg
     5461 aacaccagtg cgttgcggta gccgcgctca tgcgccgcct cgaagaagtt cgagccgacc
     5521 agcatttgcg aagcgccagc gcattccccg cctccgagtt gcaggacctt gaagtcctga
     5581 ttatcgccat ggtcacgtag caccgaaccg acttccgttt ccggcacctg caccaaatcg
     5641 gcaagcaatt cagtaaagaa cgggacgccg cggcgccgcg tccaggcaaa aaaactttca
     5701 ttcggcagat gatcctgctc gaaggtcagg cgcacccgtt ccagcgcctt ttcaatgcgc
     5761 gccgcaggca ccgacggccc tttaaacgcc aaccctccgc cggcgagacc attgccgccg
     5821 aaataggtct ggtaatgagg aatgagcttg ccatgcatgc gcctgccctc gccataaatg
     5881 ccgatatcgc ccatttccgg ctgcgcacag ccgttgtggc agccggaaac gcggatgcgc
     5941 aaatcaccgc cgacggcgtt cctgagcttg gggccaagca gcgtggacga agtgatgccc
     6001 aggcgacaag tcgaggtgcc tgggcaggct accacgttat ctcccgcttc cggctcacgc
     6061 agtccgagcg ctgcaatcgc ctggcgcagc gaattcagtt tcgcttcggg cacgtttacc
     6121 accatcaggt tctgatcctg ggttgtgcgg atttcatcca agccgtgctc caccaacgca
     6181 tgggccagcc cccgcaactg cgtcgcgttc aggttgccaa tcggcacgcc gagcggaagc
     6241 acgaacaggc cgggctgctt ttgcggcaca gtatggcgcg gcgcgccctg tccgcagatt
     6301 tcgccttcgc cgccggtctt ccattcgccc ctgggataag gctggttggc cagcgcctct
     6361 ctggtccgcg cgagttcctc acggtatttt tcaaggaaac cctcccgccc gaagcggtcc
     6421 accaggaact tgatgcgcga tttggcgcgc ttgacacggt cggaatattt gttgtgcaac
     6481 gtcacgatgg cttccatcgt gtgcagcaaa tccttttctt cgataaattc ctcgaccacg
     6541 atggcttcgt gcggcttgtg gccaagcccg ccgcccgcca gcaccttgaa gccgaaacgg
     6601 tcttctttct tcaccgcgac aatgcccatg caatgcagca tgccctgcgc gcaatcggct
     6661 tcgcagccgg aaaaactgat tttgaatttg cgcggcagct gctggttgag cggattcctc
     6721 agaaaatgct gcacggcgcc gtccagatgt ttggtcacat cggtatgctc gcgcggacag
     6781 atcccggcca ttgggcaggc ggtgacgttg cggatggtgt tgccgcaggc ttcgcgcgtg
     6841 gtgagccccg catcggccag cgtgcgcagc accgtcggcg tgtgccgagt gggcacgtag
     6901 tgcatctgaa tgtcctggcg cgtagtgatg tgcaccacgt catgctcgga atatttttcc
     6961 agcaggtcgg caatcgtgct caattgatgc gcatgcatgc gtccgccggg aatcttgatg
     7021 cgcaccatgt gcacgccttc ctggcgctgg ccgtaaacgc ccatctgcag gcgcgtcgcg
     7081 gtaaagcgtt cggcgtccag ttgcaggttc aggaaatcat cgaccgcgcg cgcgtaagct
     7141 tcaatttcct cgcggtccgc cagattgtga aaagccatcg tttatgactc cttaaattaa
     7201 atcttcacca caccagtctg ccgccgatca gcactagcat actggcgagt atctgccgca
     7261 gcacccgctc cgggattctc acgctcatgt ggctgccgag ataaattccc ggtagcgaac
     7321 ccagcaacag gctgcccagc agcgtcaaat ccaccgtgcc caatgccatg tgcccaagcc
     7381 ccgccaccgc cgtcaacggc accgcatgag cgatgtcggt gccgacgatt ttcaccgccg
     7441 gcaggcgcgg gtagaggaaa gtcagcgccg ccacgcccag cgcgcccgcg cccaccgaag
     7501 aaatcgtcac caaaactcca aggaccaaac cggtaacgat ggtggctgga agcggatggc
     7561 gctcgtgcca gagttcgccg acgtggtgct ttacgaattt ctgcagccgc tcgcggaaaa
     7621 tcagcaccag cgcggtgagt atcagcgcca cgcccagggt ggtggtaatc agggaattaa
     7681 agtgcgcttt ctctactgca aaacttttta atgcccagac cgtaatcagc gacgccggca
     7741 gactgcccaa ggcgagcagt cctacgattt tccagtccac gttgctgcgt cggccgtgca
     7801 cccagacgcc gcccatcttg gtgatggcgg cataaagcaa atccgtgccg acagcagttg
     7861 ccggcacaat gccgaacaac agcacgagta gtggcgtcat caaggagccc ccgcccacac
     7921 cggtgagccc gacgatgaag ccgaccacaa agcccgaaat cgtgtacaaa aaatccatag
     7981 tttttaagtt gttagcaatt gccgccggtt acccggaacg gccgaaaaaa gcatcgtatc
     8041 agtctctata tatatttcaa aataacatta tgttaggatg ttataacttg gggttatttg
     8101 taagggtagc aaggtatgaa gctgcagcaa ctgcgctact tgtgcgaagt cgtacgccaa
     8161 ggccttaatg tctccgaagc ggcggaaaaa ctgcacactt cgcagcccgg catcagcaag
     8221 caaatccggc tattggaaga agaacttgga gtgcagattt tcgtgcgcaa cggcaagcgc
     8281 gtggt
//
LOCUS       DATIOW010000055         1589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_428215, whole genome shotgun sequence.
ACCESSION   DATIOW010000055 DATIOW010000000
VERSION     DATIOW010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428215"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VLL03_01645"
     CDS             <1..785
                     /locus_tag="VLL03_01645"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HSS46103.1"
                     /translation="AQKKIDGLTSNVVSLQELLGDKRSRGAFGEVQLEALVRNILPPS
                     AYAMQYTLSNGSRADCVLRLPEPTGMVAVDSKFPLENYHRMFAPDVSEIERAQAQRLF
                     KADVKKHVDDISQKYIVPNETSDGAVMFVPAEAVFAEIHAYHSDIVDYAMQKRVWIVS
                     PTTLMAVLNTARAVLKDVEMRKHMNVIKEALAKLSMDFERFDVRMKNLANHIRQAHED
                     AQEVHTTSRKISDKFKRIEGVELEQNEPAAMLDVKQGREESG"
     gene            1201..>1589
                     /locus_tag="VLL03_01650"
     CDS             1201..>1589
                     /locus_tag="VLL03_01650"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HSS46104.1"
                     /translation="MWGLKAWRRQRNSSHAVLDKALWQNVVSRFVFLRTLTPGEQGRL
                     RDLTQRFLGCKQMSGAAGLQLTDEMRMVIAAQACLLILNLGLDYYRGWVEVIVYPGDF
                     MPRREYTDDDGVVHVTREPMQGEAWLQG"
BASE COUNT          355 a          436 c          487 g          311 t
ORIGIN      
        1 aggcgcaaaa gaagatagac gggctcacca gcaacgtggt gagcctgcag gagttgcttg
       61 gcgacaagcg ctcgcgcggc gcttttggtg aagtgcagtt ggaagcattg gtgagaaata
      121 tcctcccgcc ttctgcttac gccatgcaat acaccttaag caacggcagc cgcgcggatt
      181 gtgtgctgcg gcttccagag cccaccggca tggtcgcggt ggattccaaa tttcctctcg
      241 aaaactatca ccgcatgttc gcgccggatg tcagcgaaat cgaacgcgcc caggcgcagc
      301 gcctgttcaa ggcggacgtc aaaaaacacg tggacgacat cagccagaaa tatatcgttc
      361 ccaatgagac ttcggacggc gcggtgatgt ttgttccggc ggaagcggtg tttgctgaaa
      421 tacacgctta tcactccgac atcgtggact acgcgatgca aaagcgggtg tggatcgttt
      481 ccccgaccac gctgatggcg gtgctgaata ctgcccgagc ggtgttaaaa gatgtggaaa
      541 tgcgcaagca catgaacgtt atcaaggaag cgcttgccaa attgagcatg gattttgaac
      601 gttttgatgt gcgcatgaaa aaccttgcca accatatccg ccaggctcat gaagacgctc
      661 aagaagtgca caccaccagc aggaagatca gcgacaaatt caagcgcatc gaaggtgtgg
      721 aactggagca gaacgagccg gccgccatgc tggacgtgaa gcagggacgc gaagagtcgg
      781 gctgagcgtt taggaacctc tgattaagtc ccacgagggc gcgacgctgg cttttcggga
      841 tgagatgcgc gaagcgaggc gcagcgaatg gttgaccgcc ttcgcaagcc gagcgacaaa
      901 gcagatcgct cgaaaaggcg tcccggactc ggtctcgcgc agcgtggtgc gggaaggggc
      961 gcaagctgcc gcaagaaaca ccgtcaaacg gggcgctcat gctgccgctc ccgcacccca
     1021 agcttcgctg accatgtcgc ggcagccgtc ccgaccgggt ttcggcgcaa tgcggcgctt
     1081 gcgtcgttgc gctcctcgtg cttaagccac ggctatgcac cttcgtcgcg cgccttgcaa
     1141 tcatccgcat tgcgccagaa acgcgctccg cggaggactt aatcagaggt tccttaagcg
     1201 atgtggggac tcaaagcttg gcgcaggcag cgtaattcaa gccacgccgt attggataaa
     1261 gcgttgtggc agaacgttgt ttcgcgtttt gttttcctac gcacactcac gcccggtgag
     1321 caagggcgcc tgcgcgacct gacgcagcgt tttctcggct gcaagcaaat gagcggtgcg
     1381 gccggactgc agctgacgga tgaaatgcgc atggtgatag ccgcccaggc ctgcttgctg
     1441 attctcaacc tgggcctgga ttactatcgt ggctgggtcg aagtcatcgt ttatcccggc
     1501 gattttatgc cgcggcgcga atacactgat gatgacggcg tggtgcatgt tacgcgcgaa
     1561 cccatgcagg gggaagcctg gctgcaagg
//
LOCUS       DATIOW010000056         3115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1851248, whole genome shotgun sequence.
ACCESSION   DATIOW010000056 DATIOW010000000
VERSION     DATIOW010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1851248"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="VLL03_01655"
     CDS             complement(<1..1060)
                     /locus_tag="VLL03_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSS46105.1"
                     /translation="MSRWEKLKRNPRAAWAAFILLAALLAVLPFLADTLLSRTWVRIL
                     DFALLYIILALGLNIVVGFAGLLDLGYVAFFAVGAYLYALLASPHFGIHLPFWALLPL
                     GAGVTCIFGVLLGAPTLRLRGDYLAIVTLGFGEIVRIFLNNLNAPVNITNGPQGVNKI
                     DPVSFGGFSLNQTHQLLGIDFSSVHNYYYLFLILTLLVIFISMRLQDSRIGRAWVAVR
                     EDEVAAKAMGINTRNIKLLAFAMGASFGGVGGGLFAGFQGFVSPESFTLIESIMILCM
                     VVLGGMGNIAGVVLGVVLLVVLPEALRYIGPLERALFGRIIVDPADLRMLIFGLALIL
                     VMLFRPAGLWPSTTRKREF"
     gene            complement(1057..1980)
                     /locus_tag="VLL03_01660"
     CDS             complement(1057..1980)
                     /locus_tag="VLL03_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HSS46106.1"
                     /translation="MDIFLQQIINGVVLGSVYALVALGYTMVYGILGLINFAHGEVVM
                     IGAMVALAVINALTGSALPGPVIVLLGLLVAMPACMALGYGIERVAYRPLRHAPRLAP
                     LITAIGMSIVLQNSAMIIWGRQYITFPPLLPSKPHFIAGAAISDLQIFIVLLAALIMA
                     GLLFLVHRTRLGRAMRATAQNAEVAGLMGVNINTIISITFVVGSALAAVAGVMVSGYY
                     GLAHYYMGSSLGLKAFTAAVLGGIGNFGGAMLGGLLLGIIESLGAGYIGPLTGGFLGS
                     NYQDVFAFFVLILVLVFRPSGLLGERIAERA"
     gene            complement(2563..>3115)
                     /locus_tag="VLL03_01665"
     CDS             complement(2563..>3115)
                     /locus_tag="VLL03_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HSS46107.1"
                     /translation="KGKQPDLVFYGGMDAQAGPMVKQMRSLGLNAKFLGGDGTQSVEF
                     IKLAGADAEGVTASSPGLPLGKMPGGSEFLKKFESKYGKIQIYAPYAYDAAMTLVVAM
                     KKADSVEPAKYLPVLAQIQRKGVTGPIAFDAKGDVKGGAVTLYQVKGGNWVPLQTLGQ
                     QAPPAQQDTTAPASTTGSTSQGY"
BASE COUNT          697 a          952 c          916 g          550 t
ORIGIN      
        1 cgaattcgcg cttgcgggtg gtggaaggcc acaggccagc agggcggaac agcatgacca
       61 aaatcaatgc cagtccaaat atcagcatgc gcaaatcagc cggatcgaca ataatgcgtc
      121 cgaatagcgc ccgttccagc gggccgatgt agcgcagcgc ttcaggcagc accaccagca
      181 gcaccacacc cagaaccacg ccggcgatat tgcccatgcc gcccagcacc accatgcaca
      241 gaatcatgat ggattcgatg agggtgaagc tttccgggct gacgaagccc tgaaacccgg
      301 cgaacagccc cccgcccact ccgccgaagc tcgcgcccat ggcaaaagca agcagcttga
      361 tgttgcgcgt gttgatgccc atggctttcg ccgccacttc atcttcgcgt accgccaccc
      421 aggcgcggcc gatgcgcgaa tcctgcaagc gcatggaaat gaaaatcacc agcagggtca
      481 ggataaggaa tagataatag tagttatgca ccgaggaaaa atcgatgccg aggagctggt
      541 gcgtctggtt aagcgaaaat ccgccgaaac tcaccggatc gattttgttc acgccctgcg
      601 ggccgttggt gatattgacc ggcgcgttca agttgttgag gaagatacgg acgatttcgc
      661 cgaagcccag cgtgacgatg gcgaggtaat cgccgcgcag ccgaagcgtg ggtgctccga
      721 gcagcacgcc gaaaatacag gtcacaccag cgcccaaggg caataacgcc cagaaaggca
      781 ggtgaatgcc gaaatgcggc gaggcgagca gcgcatacag ataagcgccc accgcgaaga
      841 acgcgacata gcccaaatcc agcagcccgg cgaagccaac gacgatgttg agccccagcg
      901 ccagtatgat gtagagcagt gcgaaatcga ggatgcgcac ccaagtacgg ctgaggagag
      961 tgtcggcgag gaagggcagt actgcgagca atgccgctag caggatgaac gcagcccaag
     1021 cggcgcgcgg attgcgtttc agtttttccc agcggctcat gctcgttccg cgattcgctc
     1081 gccaagcaag ccggaggggc ggaacaccaa taccagaata agcacgaaaa atgcgaacac
     1141 atcctggtag ttgctgccca gaaatccgcc ggtgagcggc ccgatgtagc ccgcacccag
     1201 gctttcaatg atgccaagca atagtccgcc cagcattgcc ccgccgaaat tgccgatgcc
     1261 gcccagcacc gccgcggtaa acgccttgag ccccagagaa gagcccatgt aataatgcgc
     1321 caggccgtaa tagccgctca ccatcacgcc cgccaccgcg gccagcgccg agcccactac
     1381 aaaagtaatc gatatgatgg tattgatatt gacgcccatc agtcccgcga cttcggcgtt
     1441 ttgcgcagtg gcgcgcatcg cccgccccag gcgcgtgcgg tgcaccagga acaacaaccc
     1501 cgccatgatt aaagctgcaa gcagcacgat gaaaatctgc agatcggaaa tggcggcgcc
     1561 ggcgatgaaa tgaggcttgg agggaagaag cggaggaaag gtgatgtact gccggcccca
     1621 gataatcatc gcgctgtttt gcagcacgat ggacatgccg atggcggtga tcaggggcgc
     1681 gagccgcggc gcgtggcgca gcgggcggta ggccacgcgc tcgatgccgt agcccaatgc
     1741 catgcaagcc ggcatggcga ccagcagtcc cagcaaaaca atcaccgggc cgggaagcgc
     1801 actgccggta agcgcgttaa tcacggcgag cgccaccatc gcgcctatca tcaccacctc
     1861 gccgtgagca aagttaatca ggccgagtat gccgtagacc atggtgtaac ccaatgccac
     1921 taaggcatag acactgccta ggaccacacc attgatgatt tgctggagaa atatgtccac
     1981 gaggttctac tatatacatc aaaacggcaa agggaacctc tgattaagtc ctatacgtgc
     2041 gcgacggtgt gttttccggt ttccgagcgc gaagcgagga gcgccgttgg gtgatttccc
     2101 aatcaacgac gagcgacaaa gtcatacgac cgggcgcccc ggactcggac tcgcgcagcg
     2161 tggtgcggga ggggcgcaag ctggcgcagg aaacatcgta aaacggggcg ctcatgctgc
     2221 cgctcccgca ccccaagctt ggcttaccgt gtcgcgggcg ccccggactc ggactcgcgc
     2281 agcgtggagc ggggaggggc gcaagctgcc gcaagaaaca tcgtaaaacg gggcgttcat
     2341 gctgccgctc ccgcacccca agcttcgctt accgtgtcgc ggcagccgtc caccgggttg
     2401 cggcgactga acggccgtct gcggcgttgc tcgggcttgc gttactacga agtagcgcat
     2461 cgccctcgcg ccttgcatcc tgctgttcct cgccagcaac gcgttacgcg gagggcttaa
     2521 tcagatgttc cttaggtgcc gtggtgtcaa ggccgatttc gtttagtaac cctgactcgt
     2581 cgagccggtg gtgcttgccg gagcggttgt gtcttgctgc gccggcggcg cttgttgtcc
     2641 cagcgtctgc agcggcaccc agtttccgcc tttgacttgg tagagagtca ccgcaccgcc
     2701 tttgacgtcg cctttggcat cgaaggctat gggcccggtc acgcccttgc gctgaatttg
     2761 cgcgagcacc ggaagatatt tggccggctc aaccgaatcg gcttttttca tcgccacgac
     2821 cagcgtcatg gccgcgtcat acgcgtacgg cgcgtaaatc tggattttgc cgtatttcga
     2881 ttcaaatttt ttgagaaatt cgctcccgcc cggcattttt cccagcggca gtccgggcga
     2941 agacgcggtc acgccttcgg catccgctcc cgcaagtttg ataaattcga cgctttgcgt
     3001 gccgtcaccg ccgaggaatt tggcgttcag tcccagcgat ctcatctgct tcaccatcgg
     3061 ccccgcttgc gcgtccatgc cgccatagaa aaccagatcc ggctgcttgc ctttg
//
LOCUS       DATIOW010000057         6375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_22353, whole genome shotgun sequence.
ACCESSION   DATIOW010000057 DATIOW010000000
VERSION     DATIOW010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22353"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="VLL03_01670"
     CDS             <1..1341
                     /locus_tag="VLL03_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HSS46108.1"
                     /translation="QQALVKAQGKFVHARAGAILELENFEAYRDAAAGIRDRVIRDLD
                     LYLEQFEKNAAARGTVVHWAETAQEVNRIVCEIAELHDVRKAVKSKSMVSEECALNEA
                     LQAAGVEVVETDLGEYILQLAHEPPSHIVAPVVHKTKNEVSDLFEQKHNKPRKTGIPE
                     LCREAREVLRPHFLSADMGISGANFLIAETGSTLIVTNEGNGRLVTTLPRVHVAITGI
                     EKVVPTLEDIATLLRLLPRSATGQAITNYVSINTGVKGSDESDGPEHFHIVLVDAGRT
                     KLLGGAMQSMLRCIRCGACMNHCPVYQNIGGHAYGWVYPGPMGSVLTPVYAGLENALD
                     LPNASTLCGECAVVCPVKIPLPDLMRRLREQQVEQDLRPGLERLALKIWAWAALRPGI
                     YATLTQIAARALKWLGGEEKLLHYLPGGYGWTNGRDMPAPEGRTFREQYQERMR"
     gene            1345..2853
                     /locus_tag="VLL03_01675"
     CDS             1345..2853
                     /locus_tag="VLL03_01675"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HSS46109.1"
                     /translation="MNKPVETISLLPLWIGGGKVVSQSARFGEVTNPATGEVIRRVPF
                     ANGADIDAAVAAAKQAFPAWSETPPLRRARILMRFLQLLEKNHEQLARLASEEHGKTL
                     ADAAGSVQRGIEVVEFACGIPHLLKGEHSENVGTGVDCYTLKQPLGVCAGITPFNFPV
                     MVPLWMFPVAIACGNTFVLKPSEKVPSASLFMAELFKEAGLPDGVFNVIHGDKEAVDA
                     ILHCPDIKAVSFVGSTPVAKYIYETCAKHGKRVQALGGAKNHAVVLPDADLEFTADAL
                     IGAAYGSAGERCMAISAVVAVGEAADPLVAKLRDKTAKLKVGPGHIKEVEMGPLVTRG
                     HRDRVKSYIDLGEKEGAKLVLDGRGLKVAGFECGFYLGPSLFDHVTPQMAIYRDEIFG
                     PVLVLLRVKDFDAAIKLVNSNPQANGTAVFTRSGALARRFQHEIEVGMVGINVPIPVP
                     MAFFSFGGWKNSLFGDLHVHGVEGVKFYTRTKTVTGRWPEQAEKPKLEMPTH"
     gene            2868..3509
                     /locus_tag="VLL03_01680"
     CDS             2868..3509
                     /locus_tag="VLL03_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010116387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HSS46110.1"
                     /translation="MGARENILDRIRTALGKSASRAECEEINAYMRQHAAGPRPRSDW
                     DLTARFRERAMSLASSVDRVASISEVPQAVTRYLHERNLPLQGVCWPELASLDWQAHG
                     VSLIARPAQGNDLLGITGAFCAIAETGTLVLLSGENTPAATSLLPETHVAVVRTSRIV
                     KGMEEAWSLLRGEYPVMPRAVNFVSGPSRTADIEQTVTLGAHGPYRVHIVLVS"
     gene            3681..4883
                     /locus_tag="VLL03_01685"
     CDS             3681..4883
                     /locus_tag="VLL03_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSS46111.1"
                     /translation="MPELNFLPTLPPALTPPILFGLLLLVGLLGGEVARLFALPRITG
                     YVLVGMLFGPVGLGVLSSELIRQSRVFVDISLGLILFELGRRLDYQWLRRNRWLFATA
                     IAESAFAFWAVYFGLRYFDYPPLYAALAAAIGIATSPAVVLLVAHELRSEGQLTERML
                     LLTAVNSVFAFFSVTMLLPFLHLEQQAGWAETLPQPLYVMLGSLLTGLVVSFLVLRFA
                     AWLGKREDRQFIMLVGMIVLTAGLARTLNLSVLVTLLTLGILARNLDFRHKLMEVQFG
                     HGGQLFFVILFVMAGAGLRFDALPQVGFIAGMYIVLRFLGKALAILVFGRLSGLRPGA
                     AGLLSIALLPMSGLAIVMVQDASSLYPEFGAKLSAVVLSAVAILELIGPLATQFALKQ
                     AGEAGERR"
     gene            4880..6007
                     /locus_tag="VLL03_01690"
     CDS             4880..6007
                     /locus_tag="VLL03_01690"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HSS46112.1"
                     /translation="MTLEFTQSPAFTMGVELELQILKSHDCNLARDAADLLARLGKVK
                     HPGQVKPEITESMIELNSSIHQRYTTLADELRTVRSVVARQAELLNLRIAGGGSHPFH
                     QWSERRIYPTERFKHLLNLYGYLAKQFTVFGQHIHIGCSSGDDAVYLIHMLSRHIPHF
                     IALSASSPFYQGEDTGFASSRLNAISAFPLSGTMPFVESWDEFNAYFDRMYRFKIVES
                     MKDFYWDIRPKPEYGTVEIRICDTPLTVDKAALLAAFAQALAHYYLTERPQQATQNLY
                     LVYNYNRFQACRFGFAANFINPYDQQHANLCDDLIVTLKRLAGHSAALESNTALSQLL
                     DEVAARRNDSSWLREQLKKSGSLNDVARLQSNLWMGKPERA"
     gene            6061..>6375
                     /locus_tag="VLL03_01695"
     CDS             6061..>6375
                     /locus_tag="VLL03_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46113.1"
                     /translation="MAFPEKVKTRSAQRQYLLLSAASLVLVSNLACAAEAGGIVAKDG
                     PALFGIPIEFILFALTLLGVALFHRHTLRVALTGLAVILGYKFLFVGFKYGPGFAGFV
                     FHM"
BASE COUNT         1265 a         1771 c         1926 g         1413 t
ORIGIN      
        1 cagcaggcgc tggtcaaggc gcagggcaag tttgttcatg ctcgcgccgg tgctattctc
       61 gagctggaga atttcgaggc ataccgcgat gccgctgccg gcatacgcga ccgcgtcatt
      121 cgcgacctgg atctttatct cgagcagttt gaaaaaaacg cagccgcgcg cggcacggtg
      181 gtgcattggg cggagacggc gcaagaggtg aatcgcatcg tctgcgaaat cgccgaactg
      241 catgacgtgc gcaaagcagt caaatcaaaa tccatggtga gcgaggagtg cgcgctcaac
      301 gaggcgctgc aagcggcggg agtcgaagtg gtggaaaccg acctcggcga atacatcctg
      361 cagcttgcgc atgagccgcc ctcgcacatc gtcgcgccgg tggtgcacaa gaccaagaac
      421 gaagtttcgg atctattcga acagaaacac aacaaacctc gcaagaccgg cataccggaa
      481 ctttgccgtg aagcgcgcga ggtgctgcgc ccgcattttc tttccgctga catgggtatt
      541 tccggcgcca attttttgat tgctgaaacc gggtctacac tcatcgtcac caatgaaggc
      601 aacggccgcc tggtgaccac cttgccgcgc gtgcatgtgg cgattaccgg catcgagaaa
      661 gtagtgccga cgctggagga tatcgcgacc ttgctgcgtc ttttgccgcg ttcagccacc
      721 gggcaggcga ttaccaatta cgtttccata aataccggag taaaaggaag cgatgaaagc
      781 gatgggcctg agcatttcca tatcgtgctg gtggatgcgg gacgcaccaa attgttgggc
      841 ggtgctatgc aaagcatgct gcgctgcatc cgctgcggcg cgtgcatgaa tcactgcccg
      901 gtctatcaga atatcggcgg ccacgcctac ggctgggtgt atcccgggcc gatgggctcg
      961 gtgctgacgc cggtctatgc cgggctggaa aacgcgctgg atttgcccaa tgcatccacc
     1021 ctgtgcggcg agtgcgcggt ggtgtgcccg gtgaaaatcc cgctccccga tttgatgcgc
     1081 agactgcgcg agcagcaggt ggaacaagac ctgcgtccgg ggctggaacg acttgcgttg
     1141 aaaatctggg cgtgggcggc tctgcgtccc ggaatttatg caacgctcac gcagatcgcg
     1201 gcacgcgcac tgaaatggct gggcggcgaa gaaaaattgt tgcattatct tcctggaggg
     1261 tacggctgga ccaatgggcg cgatatgccg gcacctgaag gccgtacctt ccgcgagcag
     1321 taccaagagc gaatgaggtg agcaatgaac aaacctgttg aaaccatatc tctactgcct
     1381 ttgtggatcg gcggcggcaa agtggtgagc caaagcgcgc gctttggcga ggtgactaat
     1441 ccggcgactg gcgaagtgat ccgccgcgtg ccatttgcca acggcgcgga tatcgatgcc
     1501 gcggttgccg cagccaaaca ggcgtttccc gcttggagcg agactccgcc gctgcggcgt
     1561 gcgcgcatct tgatgcgttt cctgcagctt ctcgaaaaaa accatgaaca actggcgcgg
     1621 ctcgcgagtg aagaacacgg caaaacgctt gccgatgccg caggctcggt gcagcgcggc
     1681 attgaagtcg tggaattcgc gtgcggcatt ccgcatttgc tcaagggtga acattcagaa
     1741 aatgtaggca ccggcgtaga ttgttatacg ctcaagcagc cgcttggcgt gtgcgccgga
     1801 atcacgccgt tcaattttcc ggtgatggtg ccgctgtgga tgttcccggt ggcaattgcc
     1861 tgcggcaata cgtttgtatt gaaaccttcg gaaaaagtgc cctcggcgag cctcttcatg
     1921 gctgaactgt ttaaagaagc gggtttgccg gatggcgtgt tcaacgtgat acacggagac
     1981 aaggaggcgg tggatgcaat tttgcattgt cctgatatca aggccgtgtc cttcgtcggt
     2041 tcgacgccgg tcgccaaata catttatgaa acttgcgcga agcacggcaa gcgggtgcag
     2101 gcgctgggcg gcgcgaaaaa tcacgcggtg gtgctgcctg acgccgatct ggaattcacc
     2161 gcggacgcct tgatcggcgc ggcttatggt tcggcgggag aacgctgtat ggccatttcc
     2221 gctgtggtgg cggtgggcga ggcggccgat cccctggtgg cgaaattgcg cgacaagacg
     2281 gcgaagctca aagttgggcc gggacatatt aaagaagtgg agatgggacc gctggtgacg
     2341 cgcggccatc gcgaccgggt gaagagttat attgatctcg gcgagaagga aggcgcgaag
     2401 ctggtgcttg acggtcgtgg cctcaaggtt gccggctttg aatgtggttt ttatctcggc
     2461 ccttcgctgt ttgatcacgt gacgccgcaa atggcgattt accgcgacga aattttcggc
     2521 ccggtactgg tgctgttgcg ggtgaaggat ttcgacgcag ccatcaagct ggtcaacagc
     2581 aatccccaag ccaacggcac cgcggtgttt acacgctctg gcgctctggc gaggcgcttc
     2641 cagcatgaaa tcgaagtggg catggtcggc atcaacgtgc caattccagt gccgatggcg
     2701 ttcttctcct ttggaggctg gaaaaattcg ctgtttggcg atttgcatgt gcatggcgtg
     2761 gaaggcgtga agttctacac ccgcaccaag acggtcacgg gccggtggcc ggagcaggct
     2821 gaaaaaccca agctcgaaat gcccacccac taactagagg acaaatcatg ggcgcacgtg
     2881 aaaatatatt ggatcgcatc cgcaccgcgc tcggcaagag cgcaagtcgc gccgagtgcg
     2941 aggaaattaa cgcttatatg aggcaacatg cggcaggccc cagaccgcga tcggattggg
     3001 atctcactgc gcgctttcgc gagcgcgcta tgagtttagc gagtagcgtg gatcgggtcg
     3061 cgtcaatcag cgaagtgccg caggcggtga cgcgttatct tcatgagcga aaccttccgc
     3121 tgcaaggtgt gtgctggccg gaactggctt cattggattg gcaggcgcat ggcgtgagcc
     3181 tgatcgcgcg tcccgcccaa ggcaatgact tgctcggtat caccggcgcg ttttgcgcca
     3241 tcgccgaaac cggcaccttg gtgttgctct caggtgaaaa cacccccgcc gcgaccagcc
     3301 tgctgccgga aacgcatgtt gcggtcgtca gaacttcacg cattgtaaaa ggcatggagg
     3361 aagcttggag cttgttgcgc ggagaatatc ctgtaatgcc acgagcagta aactttgtct
     3421 caggtccgtc caggacggca gatatagaac aaaccgtcac gcttggtgcg catgggccgt
     3481 atcgagtaca cattgttctg gtcagttgaa ccttaaggac ggaagggcgg aaaaatttgt
     3541 ttacccgaaa aaacagccgc tgggtataat ctttccaact tcaaatagca gttcctaaaa
     3601 tagcagttcc tgtctcatgc ggacgtgaaa tcactgttca cgcgcacgca tccgttgttt
     3661 catttcattg ttgaaccaac atgccagagc tcaactttct gccaactttg ccgcccgcac
     3721 tcacaccgcc catcctgttc ggattgttgc tgctggtggg tttgctcggg ggtgaagtgg
     3781 cacggctgtt cgcgctgccg cgcatcaccg gatatgtcct ggtgggcatg ctgttcggcc
     3841 cagtaggcct tggcgtattg agcagtgagc tcatccggca aagccgggta tttgtggata
     3901 tttcgttggg cttgattctg ttcgagctcg gccgccgcct tgattaccaa tggctgcgcc
     3961 gcaaccgctg gctgttcgcc accgcgattg cggaaagcgc gtttgccttc tgggcggttt
     4021 atttcgggct gcgttatttc gattacccgc cgctctatgc cgcgcttgcc gcggcgatcg
     4081 gcattgccac ttctcctgcg gtggtgttgc tggtggcgca tgagttgcgc tcggaagggc
     4141 agcttaccga gcgcatgctg ctgctcaccg cggttaacag cgtgttcgcc ttcttcagtg
     4201 tgactatgct gctgccgttc ctgcatttgg aacagcaagc cggatgggcg gaaacgctgc
     4261 ctcaaccgct ttatgtcatg ctgggttcgc tgctcacagg tctcgtcgtg agtttcttgg
     4321 tgctgcgttt cgccgcctgg ctgggcaagc gcgaggaccg ccagttcatc atgctggttg
     4381 gcatgattgt gctcacagca ggattggcgc gcacgctcaa tctttcggtg ctggttacac
     4441 tgcttaccct cgggatactc gcgcgcaatc tcgatttccg ccacaagctg atggaggtgc
     4501 agttcggtca tggcggacag ttgttttttg tgattctttt cgtgatggcg ggcgcgggcc
     4561 tgcgcttcga tgcactgccg caggtggggt tcatcgccgg tatgtacatc gtgttgcgtt
     4621 tcctcggcaa ggcgctggcg atcctagtat tcggccgttt aagcgggctg cgcccggggg
     4681 cggcgggcct gctcagcatt gccttgctgc ccatgtcagg gctcgccatt gtcatggtgc
     4741 aggacgcctc cagtttgtat cctgaatttg gcgccaagtt gtcggcggtg gtgctttccg
     4801 cagtggcgat actggagctt atcggtccgc tggcgactca gtttgcgctg aaacaggcgg
     4861 gcgaggccgg agaaaggcga tgacgctgga attcacacaa tcgccggcgt tcaccatggg
     4921 cgttgagctt gagctgcaga tcctcaagtc ccacgattgc aatctggcgc gcgatgccgc
     4981 tgatttgctg gcgaggcttg gcaaagtgaa acatccgggc caggtcaagc cggaaatcac
     5041 cgagagcatg atcgagctca attcctcgat ccaccaacgc tacacgaccc ttgcggacga
     5101 gctgcggact gtccgcagtg tggtggcgcg ccaggccgag ctcctcaacc tacgcattgc
     5161 cggcggaggc tcgcatccct ttcaccagtg gagcgagcgg cgcatttatc cgaccgagcg
     5221 tttcaagcac ctgcttaatc tctacggcta tctcgccaag cagttcacgg tattcgggca
     5281 gcatatccac atcggctgct cgagcggcga tgacgcggtc tatctcatcc acatgctctc
     5341 gcgccatatt ccgcatttca tcgcgctttc cgcctcatcg ccgttttacc agggcgaaga
     5401 caccggtttc gcatcttcgc ggctcaatgc catcagcgcc tttcccttaa gcggcaccat
     5461 gcccttcgtc gaaagttggg atgaattcaa tgcctatttt gaccgcatgt atcgtttcaa
     5521 aatcgtcgag agcatgaagg atttctactg ggatatccgg cccaagccgg aatatggaac
     5581 cgtggaaatc cgcatctgcg acacgccgtt gacggtggac aaggcggcac tgctcgcggc
     5641 tttcgcccag gcgctcgcgc attattacct gactgaacgg ccgcaacagg cgacgcaaaa
     5701 tctttatttg gtttacaact ataaccgctt ccaggcctgt cgcttcggct tcgccgccaa
     5761 tttcatcaat ccttatgatc agcagcatgc caacttgtgt gacgacctca tcgtcactct
     5821 caagcgactc gcagggcatt ccgccgcgct ggaaagcaac acagcgctca gccaattgct
     5881 cgacgaggtg gcggcgcgcc gcaacgattc atcctggttg cgcgaacaac tcaaaaaatc
     5941 cggttcactc aacgatgtgg cacgcctgca gagcaacctg tggatgggaa aaccggaacg
     6001 ggcatgagcc cggcctgtgt ttcaaatcaa tgctagaatc gcgcgtccat gggagggggc
     6061 atggcatttc ctgaaaaagt caaaacgcgc tcggcgcaac gccaatattt gctgctgtcc
     6121 gcggcatctc ttgtcctggt gagcaatctc gcttgcgcgg cggaagccgg cgggattgtg
     6181 gcgaaagacg gcccggcgct gttcggtatc cccatcgaat tcatcctgtt tgcactgacc
     6241 ctcctcggcg tggcgttgtt ccatcgccac acgctgcgcg tggcgctgac cggcctcgct
     6301 gtcatcctgg gttataaatt cctgttcgtc gggttcaagt acgggcccgg atttgccggt
     6361 ttcgtctttc acatg
//
LOCUS       DATIOW010000058        14591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1751765, whole genome shotgun sequence.
ACCESSION   DATIOW010000058 DATIOW010000000
VERSION     DATIOW010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14591
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1751765"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..622
                     /locus_tag="VLL03_01700"
     CDS             56..622
                     /locus_tag="VLL03_01700"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSS46114.1"
                     /translation="MATYQELSDFLAEVERRAFKQALFAVRDEEAALDIVQDSMLKLA
                     EKYAGKPPAELPMLFQRILQNTIRDYYRRQKVRNLWTTLVSALVPGDADEEHDPLETL
                     RPVDSSNERGGPLERLEQGQIVAIIEQALETLPARQRQAFLLRYWEDMDVAETAAVMG
                     CSEGSVKTHCSRATHTLAAILKQRGVEL"
     gene            619..978
                     /locus_tag="VLL03_01705"
     CDS             619..978
                     /locus_tag="VLL03_01705"
                     /inference="COORDINATES: protein motif:HMM:NF023699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HSS46115.1"
                     /translation="MNEQELGSQIARHLDRGADQLKPGTLYRLQSARHNALEKYRPKA
                     AGWAWGGSVSWSGSMLSVKFLLPLAVLLAGLGGAIYWQNLQQSNDFAEIDFHLLTDDL
                     PINAYLDKDFEAWLNRS"
     gene            960..1397
                     /locus_tag="VLL03_01710"
     CDS             960..1397
                     /locus_tag="VLL03_01710"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HSS46116.1"
                     /translation="MAKSLLTVILLCFISTVFAAPADSKAPAWTGLTPQQKQILAPLE
                     PEWNNMEPQRRKKWIGIAERYPKMTPEQQQRLQLRMREWAKLTPQERQQAREKFKNMK
                     QLPPEKRQEIKKKWSQYQEERNRQTAPQAPADTPAPFALQPAR"
     gene            1421..1840
                     /locus_tag="VLL03_01715"
     CDS             1421..1840
                     /locus_tag="VLL03_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HSS46117.1"
                     /translation="MPDTSPAASLRRRLASLVYEALLLAAVLWAAGALFTLMVPDASP
                     PLPQGLLRAFLLAVAFAYFAWCWTRGGQTLAMKTWRIRVLSASGLPLTLKQALLRFVL
                     AVLGIGLAGCGLLWAWFDRDRQFLHDRLAGTKIVREE"
     gene            complement(1884..2957)
                     /gene="lptG"
                     /locus_tag="VLL03_01720"
     CDS             complement(1884..2957)
                     /gene="lptG"
                     /locus_tag="VLL03_01720"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HSS46118.1"
                     /translation="MRIINRFLRQHIFDSTLLVFAALVGLFALLDLIHELGDLGYANY
                     RLHHILFYVLLSVPGHVYELFPIAALIGTLLALAQLVIHSEYTVIRVSGVSARDMVLS
                     LMQAGLVFVVLTFVTGEFIAPVTERAAQQLRLKALKTVVAQEFRSGLWVKDERSFVNV
                     AQILPDTTLLGVRIFEFDPERHLRAISYAQKGDYVKDNLWRLYNVVQTRFDDEHATVN
                     SIAEANWFSVLNPNILNVLLVVPEQMSAWNLYVYTQHLRENNQKTQRHDLALWTKITY
                     PLAALVMMVLALPFAQYQRRTGGISARIFAGIMLGLGFHFLNKLFAHLGLLNDWPPFV
                     SAMAPTLLFLAGGMAMLWWVERR"
     gene            complement(2954..4036)
                     /gene="lptF"
                     /locus_tag="VLL03_01725"
     CDS             complement(2954..4036)
                     /gene="lptF"
                     /locus_tag="VLL03_01725"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HSS46119.1"
                     /translation="MIFRRVLLREFADIALTVFVILFAIVLVTQLVRFLGAAASGAVA
                     PEGVIALLGFRAIYYLPVLLALTLFISVLLSLTRSYRDSEMIVWFSSGISIAAWIRPV
                     TVFAVPMVAVIAVLSLFLSPWAFGKGEEYRNQLDSRDDVATATPGVFRESRQADRVYF
                     VGSAAGEGDTVSDIFVSSVQHQQLGTMVAKNGYQETRENGDRFLVLLHGQRYDGPPGS
                     PEYKITDFDKYEMRIEPYEAKQEAPQGRATSTLGLLREGSPASLAEFVWRAGLPISAL
                     ILALLAVPLSFVNPHVGRSFNLILALAVYMLYSNSLSIARGLVAQQKINLALGFWGVH
                     IFMLAVLALLFYKRLAVSAFMRFFKR"
     gene            4125..5615
                     /locus_tag="VLL03_01730"
     CDS             4125..5615
                     /locus_tag="VLL03_01730"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HSS46120.1"
                     /translation="MEFSIKTSAPEKQTAHCAVLGVYASRKLSPAAAAVNRASGGYLE
                     RILRRGDIQGKSGSTLLLHNVPGVAAERVLLVGLGREADFKEQSYFDALRAAVKALNE
                     TGAAEAALFLAELPVKKHDTAWKILQAALQVRDAVYRFDRLKSKSEESKKTLSKVSLM
                     VERKDFARAEAALKQGIAVAEGIQLTRDLGNLPPNICTPTYLAQQALQLAKTHKLKAQ
                     VLERRDMEKLGMGSLLSVARGSRQPPKFIVLQYHGAAKKQKPVVLVGKGITFDTGGIS
                     IKPAAEMDEMKFDMCGAASVLGSIKAVAQMRLPLNVVGIIPTTENMPGGNASRPGDIV
                     TSMSGQTIEILNTDAEGRLILCDALTYAERFDPAAVVDIATLTGACVIALGHVASGLY
                     SNDESLARELLKAGEVTHDRAWQMPLWDDYQEQLKSNFADMANIGGRPAGSVTAACFL
                     SRFTKKYAWAHLDIAGTAWKSGRDKGATGRPVPLLTQFLMSRAAKA"
     gene            5617..6051
                     /locus_tag="VLL03_01735"
     CDS             5617..6051
                     /locus_tag="VLL03_01735"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HSS46121.1"
                     /translation="MTEIDFYTHVENKLQVACSLSAKALEKGLRVMIYAADAETTEKL
                     DRLMWCYPAISFIPHCRAADPLAQETPIIIDHYSEPLPHDQVLLNLHPEWPPFFSRFQ
                     RLIEIVGLNEADSQAGRNRYRFYRDRGYDIRHHDLSKTAAEL"
     gene            6057..6482
                     /locus_tag="VLL03_01740"
     CDS             6057..6482
                     /locus_tag="VLL03_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46122.1"
                     /translation="MAKPDNLLSKMDALMKKHRAPDNEIPTLTDVIATAAKPGPGQTG
                     LAALTPEQVETLAIQIYQRVLQNLDEHINNELQLHLKPQLNILFGHVLDGMLAELKNS
                     IKAVVDDAVAQALDAQLKHHQKLDQSSAPTTKRFSGDKP"
     gene            6512..9301
                     /locus_tag="VLL03_01745"
     CDS             6512..9301
                     /locus_tag="VLL03_01745"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HSS46123.1"
                     /translation="MELAKSFDPHDIESRWYPEWERRGYFRHRSSDLSTSPAPSADAY
                     CIQLPPPNVTGTLHMGHAFQQTLMDILIRYHRMHGFNTNWVMGSDHAGIATQIVVERQ
                     LEMENKTRRDLGREKFIERVWQWKQQSGSTITRQMRRLGLSGNWDYASTDGQHAGYFT
                     MDERMSCAVIEVFVRLYEQGLIYRGKRLVNWDPVLGTAVSDLEVDSEEEDGKLWEIHY
                     PLESGGKGVVVATTRPETMLGDVAVAVNPGDKRYKSLVGKRVSLPLTGRTIPVIADEY
                     VDPVFGTGCVKITPAHDFNDYKVGQRHRLPQINVMTLDAKMNENAPEAYRGLDRFDAR
                     KCILADLKAQNSLVSEKPYKLRVPRSGRTGEIVEPMLTDQWFVKMDGLAKLGLEAVAN
                     GEVKFFPEHWTTTYNQWLENIQDWCISRQLWWGHQIPAWYDDKGNIYVARSEKEAKKQ
                     AAAKGYRGALKRDEDILDTWFSSALVPFTSLGWPEKTKDLDTFLPSSVLVTGFDIIFF
                     WVARMIMMTLHFTDKVPFHHVYINAIVRDAEGQKMSKSKGNTLDPLDLIDGVTLNALL
                     EKSTTGLLRDEHKGKIEKYVRNHYPNGIPSFGADALRFTFASLAGFARTLNFDLNRCE
                     GYRNFCNKLWNATRFVLMNCEGKDCGQDDKAAVALSTWDRWIVSRLQRTEAEVAKGFD
                     EYRFDLAAQAIYEFVWNEYCDWYVELAKVQLANGSEEEQHGTRRTLVRVLEAALRLAH
                     PIIPFITEELWQKVAPLAGKSGETVMLAPYPKSQPEKIDEEVEREVDEAKDVVNAGLK
                     QRSEIKLQPQYRVPYYMTGRPTNTTTAAFSALLRPSELHIVNELPQSDSPVAVVRSYR
                     VMPYIKIDVAAEIERIKKEIARIEDEIAKANAKLANPSFVERAPAEIIAQEKERRTNF
                     GATLEKLTAQLGKLT"
     gene            complement(9307..10005)
                     /locus_tag="VLL03_01750"
     CDS             complement(9307..10005)
                     /locus_tag="VLL03_01750"
                     /inference="COORDINATES: protein motif:HMM:NF022041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HSS46124.1"
                     /translation="MIKLYQFQPAFGLPNASPFCMKVENYLRMAGLQYEIAPNASHFK
                     APKGKLPYIEDGSKVIADSSFIIEYLKSTYGDALDAKLTPVENAQAVALKRMMEENLY
                     WALAYTRWMEPQGWEAIKAEFFKPVPALLRGPISALARRGVARQMWGQGMGRHGAEEV
                     HHIGKTDITALADFLGDKLFFMGEKPTSLDATAYAFLANIILVPLDSAFKQHALKYPR
                     LEAYCQRMRARYYA"
     gene            complement(10024..10251)
                     /locus_tag="VLL03_01755"
     CDS             complement(10024..10251)
                     /locus_tag="VLL03_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HSS46125.1"
                     /translation="MNFDRQIDTRGLNCPLPILRTKKTLAEMTSGQVLKVLATDPGSV
                     KDFQAFSRQTGNELLSQAEAGGEFIFFMKKR"
     gene            complement(10248..10574)
                     /locus_tag="VLL03_01760"
     CDS             complement(10248..10574)
                     /locus_tag="VLL03_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSS46126.1"
                     /translation="MQSIQEIDAARLQEMQSSGNLRLVDVRGDAEVARGMIKGAIHIP
                     LHLLPPKAAEWDVETPTVFYCQSGARSAQACTFFAGKGFNKVYHLQGGVNTWVRNGLP
                     LTGVAA"
     gene            complement(10603..11898)
                     /locus_tag="VLL03_01765"
     CDS             complement(10603..11898)
                     /locus_tag="VLL03_01765"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HSS46127.1"
                     /translation="MDKIGKYEIVKELGKGATSAVYLANDPFANRQVAIKLVDAKALR
                     DKQQGRRYKKLFLTEASLVGKLSHPHIVAIYDAVVEDDLSYIVMEYVAGGTLEKYCKV
                     ENLLPVDKVIEIIFKASRALAFAHRYGIIHRDIKPANILLTSDLDIKISDFGTALTEG
                     ADTTQIVGIGSPAYMSPQQVREMPLNHQTDIFSLGVVMYQLLTGKLPFSATNNYSMVY
                     QIINVDPPPPSLHRPEIPVQLDVIVKTALRKDLVTRYQTWEDFAQALIDVYNTLEQPK
                     EDFSEGERFDTLRKLSFFAHFNDSELWEVLRITNWHKYKPGITLLKEGEVGKSFFILA
                     SGEVNVSKDGKLLNVLQAGECFGEMVYLGKARFKRTASIVTVNDTTAIEIAADSLAQA
                     SENCRAQFDRTFLQILVDRLAAANTRLSQLLLDRKISIF"
     gene            11995..13356
                     /gene="chrA"
                     /locus_tag="VLL03_01770"
     CDS             11995..13356
                     /gene="chrA"
                     /locus_tag="VLL03_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019651951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HSS46128.1"
                     /translation="MDIPSRTIEPPPSVSFAEAFRYWLMLGFISFGGPAGQIAVMHHE
                     LVEKRRWISERRFLHALNYCMLLPGPEAQQLATYIGWLMHKTPGGIVAGVLFVLPSLF
                     ILIALTWIYLAFGNVPVVAGIFYGIKPAVTAIVVFAAYRIGARALKNAALWTLAAAAF
                     IAIFALKIPFPYIVLAAGIIGYFGGRLAPAKFAIGGGHGASDKSFGPTIIDDHTPTPE
                     HARLSWPRFAFYSVVGLVLWGGAIAILASLYGWEGTLTQMGWFFTKAALLTFGGAYAV
                     LPYVYQGGVEYYQWLTPAQMIDGLALGETTPGPLIMVVAFIGFVGGWSKELFGPDLSF
                     VSGAIAATVVTYFTFLPCFLFILLGGPFIETTHGDIRFTAPLTGITAAVVGVVLNLAL
                     FFAYHVLWPQGFDNRFEWLSLGIGLAAFIALTRFGIGIIPVIGCCGATGLAYTLLRFW
                     SMR"
     gene            complement(13358..>14591)
                     /locus_tag="VLL03_01775"
     CDS             complement(13358..>14591)
                     /locus_tag="VLL03_01775"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HSS46129.1"
                     /translation="LNDLGYRLISSSDEPKTSLDLFAIHDSEINAFSLPGGFIGVNTG
                     LILLTQSESELASVLAHEIGHVTQHHIARILATQKQSIATSLAGIAIAILAARSNSQL
                     SQAAIIGSQAAVAQNQLNFTRGHEQEADRIGLQTLDKAGFDVRAMPVFFERLQKATRF
                     VEGNAPSYLRTHPVTFERIADIQNRVQNMHYRQVPDSQEFQFLRARLRAQEESPKNAI
                     AYFENSLQEKKYTSEAAQRFGLTIALLRARNLERAEQELAQVRKIAQPSPLIEILAGQ
                     LKSAGGKTAEASAIYRAALKNYPQYRPLVYASCDVFLQKNQALDALNLASNQLQFYPE
                     DYHLYLLQAKSYAALDKKLLQHKSQAEAYYLLGNLRAAVEQLQIAQKSGDGDFYQLSA
                     VEARLKEFRALEVELRKQ"
BASE COUNT         3167 a         4299 c         4199 g         2926 t
ORIGIN      
        1 gatggcgcaa tcggcacatt gcaggctgcg cccacttctc gcaaggttga attaactggc
       61 tacctatcag gaactctccg attttctggc agaagtggaa cgccgcgctt tcaagcaggc
      121 gctgttcgcg gtgcgcgacg aagaagccgc gctcgacatt gtccaggact cgatgctgaa
      181 gctcgcggaa aaatacgctg gcaagccacc ggcggaattg ccgatgctgt tccagcgcat
      241 cctgcaaaac accatccgcg attactaccg ccggcaaaaa gtccggaatc tctggaccac
      301 cctggtatcc gccctcgttc cgggcgatgc cgacgaggag cacgatcccc tcgaaacctt
      361 gcgcccggtt gacagttcga acgagagggg aggccccttg gagcggcttg aacagggaca
      421 aattgttgct attattgaac aagcgctgga gactctgccg gcgcgtcaac gccaggcgtt
      481 cctgctgcgt tattgggaag atatggatgt cgccgagact gccgccgtca tgggttgttc
      541 ggaaggcagc gttaaaacgc attgttccag ggccacccac accctggccg caatcttgaa
      601 gcaacgagga gtggaattat gaacgaacaa gagttgggca gccaaatcgc ccgccatctg
      661 gaccgcgggg cggaccaact caagccgggt acgctttacc gcttgcagtc cgcacgccac
      721 aacgcgctgg aaaaataccg tcccaaagcc gcaggctggg cgtggggcgg ttctgtttcc
      781 tggtctggct cgatgttgag cgtgaagttt ctgctgccgc tcgcggtgct gttggccggc
      841 ctgggcggag cgatttactg gcaaaatctg cagcaaagca acgattttgc ggaaatcgac
      901 tttcatctgc tgaccgacga cctgccgatc aatgcttatc tcgacaagga ctttgaggca
      961 tggctaaatc gctcttaaca gtgatactgc tatgcttcat ttccaccgtt tttgccgcac
     1021 ccgctgactc caaagcaccc gcctggacag ggctcacgcc ccagcaaaag cagattctcg
     1081 ccccacttga accggagtgg aacaatatgg aaccgcagcg gcgcaagaaa tggattggaa
     1141 ttgccgagcg ctacccgaaa atgaccccgg aacagcagca gcggctgcag ctgcgcatgc
     1201 gcgaatgggc gaaactcacg ccgcaggaac gtcagcaggc gcgcgagaaa ttcaagaaca
     1261 tgaagcagtt gcccccggaa aagcggcagg aaatcaagaa aaaatggagc caataccagg
     1321 aagagcgtaa ccgccaaaca gcgcctcaag cgcctgccga cacaccggcc ccttttgccc
     1381 tccaacctgc gcgttaaggc ttgcgaagtc gggctttccc ttgccggata cttcccctgc
     1441 cgccagcctg cgccgccgcc tagccagcct tgtctatgaa gcgttgcttc tggctgcggt
     1501 gctgtgggcg gcgggcgccc tgttcaccct gatggtgcct gacgcaagtc cccctttgcc
     1561 gcaaggactg ctgcgagctt ttttgctggc agtggctttc gcttatttcg cctggtgctg
     1621 gacgcgcggg gggcaaacac tggcgatgaa aacctggcgc atccgggtgt tgagcgcaag
     1681 cggcctgccg cttacactga agcaagcact actgcgcttt gtgctggcgg ttttgggaat
     1741 cggcctggcg ggatgtggat tgctctgggc ttggttcgac cgcgaccgcc agtttctgca
     1801 cgaccggctg gcgggaacta aaattgttag ggaagaataa ggggtaaagg ttaaaacact
     1861 cggcaggttt cctcatttac tcctcacctt ctttccaccc accacagcat cgccatcccg
     1921 cccgcgagaa acagcagcgt cggggccatc gccgaaacga acggcggcca gtcgttcaac
     1981 aaacccaggt gagcgaacag cttgttcaaa aaatgaaagc ccaggccgag catgatgccg
     2041 gcgaaaatcc tggcgctgat gccgccggtg cgccgctggt actgggcaaa cggcagcgcc
     2101 agcaccatca tcaccagcgc cgccagcggg taagtgatct tggtccacag cgccaaatca
     2161 tggcgctgcg tcttctggtt gttctcacgc aagtgctgcg tatagacata aaggttccac
     2221 gccgacatct gctccggcac caccagcagc acgttgagaa tgttcgggtt cagcacggaa
     2281 aaccagttgg cctctgcgat tgagttcact gtggcgtgtt cgtcatcgaa gcgggtctgc
     2341 accacattgt acaggcgcca caggttgtcc ttcacgtagt cccctttttg cgcgtaactg
     2401 atggcgcgca aatgccgttc cggatcgaat tcgaaaatgc gcacgcccag cagcgtggtg
     2461 tcgggcagga tctgcgcaac attgacaaag ctcctttcat ccttcaccca cagcccggag
     2521 cggaattcct gcgccaccac ggttttcagc gccttgaggc gcagttgctg cgccgcgcgc
     2581 tcagtcaccg gggcgatgaa ttcgccggtc acaaacgtca gcacgacgaa caccaggccg
     2641 gcctgcatca gcgacaacac catgtcacgc gcggagacac ccgagacgcg gatcacggtg
     2701 tattcggaat ggatcaccag ttgcgccagc gccagcagcg tgccgatcag cgccgcgatc
     2761 gggaaaagct cgtagacgtg gccgggcacg ctcaatagca cgtagaacag gatgtggtgc
     2821 agacggtaat tagcgtagcc caagtcgccc agctcgtgaa tcaaatcgag cagcgcaaac
     2881 agccccacca gagcggcaaa caccagcagc gtgctgtcga aaatgtgctg cctgagaaag
     2941 cggttgatta tcctcatcgc ttaaaaaacc gcataaacgc ggatactgcg agccgcttgt
     3001 agaacaacag ggcgagcacc gccagcatga agatatgcac cccccagaaa cccagcgcga
     3061 ggttgatttt ctgctgtgcc accagcccgc gggcgatgct gagagaattg ctgtagagca
     3121 tgtacaccgc aagcgccaga atcaggttga aggagcgccc gacgtgcggg ttgacgaagc
     3181 ttaacggcac cgcgagcaaa gccagaatca gggcgctgat cggcagcccc gcgcgccaga
     3241 cgaattcggc gaggctcgcc gggctgcctt cgcgcagcag accgagcgtg gacgttgcac
     3301 gcccctgcgg cgcttcctgt ttcgcctcat acggctcgat gcgcatttcg tatttgtcga
     3361 aatccgtgat tttgtattcg ggggagccgg gcggcccgtc gtagcgctgg ccatgcaaca
     3421 gcaccaggaa gcggtcgccg ttctcacgcg tttcctgata gccgtttttc gccaccatgg
     3481 tgcccagttg ctggtgctgc accgagctca cgaaaatatc ggaaacggta tccccttctc
     3541 ccgcggcgct tcccacaaaa tacacgcggt ccgcctgcct ggattcgcgg aacacacccg
     3601 gcgtagccgt cgccacatca tcgcggctgt cgagttgatt acggtattcc tcgcctttgc
     3661 cgaaagccca cggcgaaaga aacaggctca ataccgcaat caccgccacc atcggcacgg
     3721 caaacaccgt caccggacgg atccacgcgg caatgctgat gccggaactg aaccatacga
     3781 tcatctccga atcgcggtag ctgcgggtca aactcagcag cactgaaata aacagcgtca
     3841 gcgccagcag caccggcagg tagtaaatgg cgcgaaaacc caaaagcgcg atcacgcctt
     3901 cgggcgccac cgcgccgctc gcggcggcgc cgaggaagcg caccagctgc gtcactagaa
     3961 cgatggcgaa caggatcacg aacacggtca gcgcgatgtc ggcgaattcc cgcagcaaga
     4021 cgcggcgaaa aatcatgatt gtctactcgg cttttgactt ttcacaaaaa ctctgtggat
     4081 aatgcagtgt ttaggaaaat tttttgcgaa agaaggagca aaatgtggaa tttagcataa
     4141 aaacaagcgc cccggaaaaa cagaccgccc actgtgcggt gctcggggtt tatgcatcgc
     4201 gcaagctttc accggcagca gcggcagtta accgcgcgtc cggtggctat cttgagcgta
     4261 tcctgcgccg aggcgacata cagggcaaat ccggctccac cctgctttta cacaacgtac
     4321 cgggcgtggc ggccgagcgc gtgctgctgg tgggcttggg ccgggaagcg gatttcaagg
     4381 aacagtcgta tttcgatgcg ctgcgcgcgg cggtcaaggc gctgaatgaa accggcgccg
     4441 ctgaagcggc gttgtttctc gctgagttgc cggtcaaaaa acacgatacg gcgtggaaaa
     4501 tcctgcaagc ggcgctacag gtgcgcgatg cggtttatcg cttcgaccgc ttgaaaagca
     4561 agagtgaaga aagcaaaaaa acgctttcga aggtgagcct gatggtggag cgcaaggact
     4621 ttgcccgcgc cgaagccgcg ttgaaacagg gaatcgcagt cgccgaaggc atccagctca
     4681 ctcgcgattt gggaaacctg ccgcccaata tctgcacccc gacctatctc gcgcaacaag
     4741 cgctgcagct cgccaaaacc cacaagctca aggcgcaagt actggagcgc agggacatgg
     4801 aaaaactcgg catgggttcg ctgctctcgg tcgcgcgcgg cagccgccag ccgcccaagt
     4861 tcatcgtgct gcaataccac ggcgcggcca aaaaacaaaa acccgtcgtg ctggtcggca
     4921 aaggcattac tttcgatacc ggcggcatct caatcaagcc cgcagcggaa atggacgaaa
     4981 tgaaattcga catgtgcggc gcggcgagcg tgctgggctc aatcaaggcg gtggcgcaga
     5041 tgcggctgcc gcttaatgtg gtcggcatca ttcccaccac cgaaaacatg ccgggaggca
     5101 acgccagccg tccgggcgac atcgtcacca gcatgtcggg gcagaccatc gagattctca
     5161 ataccgacgc cgaggggcgg ctgattctgt gcgacgcgct cacttacgcc gagcgctttg
     5221 atccggcggc agtggtggac atcgccacgc tcaccggcgc gtgcgtgatt gcgctcggcc
     5281 atgtcgcttc cggcctttac agcaacgatg aatctttggc gcgcgagttg ctcaaagccg
     5341 gcgaggttac gcacgaccgc gcctggcaaa tgccgctgtg ggacgattac caggagcagc
     5401 tcaaaagcaa tttcgccgac atggccaaca tcggcgggcg ccccgccggc agcgtgacag
     5461 ccgcgtgttt cctctcgcgc ttcaccaaga aatacgcttg ggcgcacctc gacatcgccg
     5521 gcacggcgtg gaaatcgggc agggacaagg gcgcaaccgg ccgtccggtg cctctgctca
     5581 ctcaatttct gatgagccgc gccgccaaag cataagatga cggagattga tttttacact
     5641 catgtcgaaa acaagctgca ggtggcgtgc agcttgagcg ccaaggcgct ggaaaaaggc
     5701 ctgcgcgtga tgatttacgc cgctgacgcc gaaaccacgg aaaagctaga ccggttaatg
     5761 tggtgttatc ccgcaatcag cttcatcccg cactgccgcg ccgccgaccc gctggctcag
     5821 gaaacaccaa tcatcatcga tcattactcc gagccgctgc cgcacgatca ggtgctgctg
     5881 aatctccatc ccgagtggcc gccgtttttc agccgtttcc agcgcctgat tgaaatcgtg
     5941 ggtctcaatg aggcggacag ccaggcgggc cgcaaccgct accgtttcta ccgcgaccgc
     6001 ggttatgaca tccgtcatca cgatctcagc aaaaccgcgg cggagttata acgcctatgg
     6061 cgaagccaga taacctcctc tccaaaatgg acgcgctgat gaaaaagcat cgcgcgccgg
     6121 acaatgaaat tcccacgctc accgacgtga tagccaccgc cgccaagccg ggcccgggcc
     6181 agaccgggct cgccgctttg acgccggagc aggtggaaac gctggcgatt caaatctacc
     6241 agcgcgtgct gcaaaacctg gacgaacaca tcaacaacga actgcagctg cacctcaagc
     6301 cgcaactcaa catcctgttc ggacatgtgc tcgacggtat gctcgccgag cttaagaata
     6361 gcatcaaggc cgtggtcgac gacgccgtgg cgcaggcgct ggacgcgcag ctcaaacacc
     6421 accaaaaact cgatcaatcc tccgctccaa cgaccaagcg cttcagcggc gacaagccat
     6481 aactgtcatt cctcccaatc cgcagtaacg catggaactc gctaagagtt ttgacccgca
     6541 tgacatagaa agccgctggt atccggaatg ggaaaggcgc ggctattttc gtcatcgttc
     6601 ctcagaccta tccacgtcgc ctgcgccgtc ggcggatgcg tactgcattc agctgccgcc
     6661 gcctaatgtc acgggcaccc tgcacatggg gcatgcgttc cagcagacgc tcatggacat
     6721 cctcatccgc taccaccgca tgcacggctt caacaccaat tgggtgatgg gcagcgatca
     6781 tgccggcatt gcgacgcaaa ttgtggtcga acgccagctc gaaatggaaa acaaaacacg
     6841 ccgcgactta gggcgcgaga aattcatcga gcgcgtctgg caatggaaac aacaatccgg
     6901 ctcgaccatc acgcgccaga tgcgccgcct gggcctctcc ggcaactggg attacgcgag
     6961 cactgatggc cagcatgcgg gctactttac gatggacgag cgcatgtcgt gcgcggtcat
     7021 tgaagtgttt gtgcggctct acgaacaggg gctgatttac cgcggcaagc ggctggtcaa
     7081 ctgggacccg gtgctcggca ccgcggtgtc cgacctggaa gtagacagcg aggaagagga
     7141 tggcaagctt tgggaaatcc actatccgct ggaaagcggc ggtaagggcg tggtggtggc
     7201 gaccacgcgg ccggaaacca tgctgggcga tgtggctgtt gcggtcaacc cgggagataa
     7261 acgctacaaa tcgctcgttg gcaaacgcgt ttcgctgcct ctcaccggcc gcaccattcc
     7321 cgtgattgcc gacgaatatg tggatcctgt attcggcacc ggctgcgtga aaatcacacc
     7381 ggcgcatgac ttcaatgact ataaagtggg tcagcggcac cggttacctc aaatcaacgt
     7441 gatgacgctt gatgcgaaga tgaacgaaaa tgcaccagag gcataccgcg gcctcgaccg
     7501 cttcgacgcg cgcaagtgca tcctcgctga cctcaaggcg cagaattcgt tggtttcgga
     7561 aaaaccctac aagctgcgcg tgccgcgttc ggggcgcacc ggagaaattg tcgagccgat
     7621 gctcaccgac cagtggttcg tgaaaatgga tggtttggcg aaacttggcc ttgaagcggt
     7681 cgcaaatggg gaagtaaagt tttttcccga gcactggacg acgacataca accaatggct
     7741 ggaaaacatc caggattggt gcatctcacg acaattgtgg tggggacacc agattcccgc
     7801 gtggtatgac gacaaaggca acatctatgt cgcccgcagc gagaaggagg cgaaaaagca
     7861 ggctgccgcc aagggctatc gcggcgcgct gaagcgcgac gaggacattc ttgacacctg
     7921 gttctcctcc gctctggtac cctttacctc gctcggctgg cctgaaaaaa ccaaagacct
     7981 cgacactttc ctgccctcct cggtgctggt caccggcttc gacatcatct tcttctgggt
     8041 cgcgcggatg atcatgatga cgctgcactt caccgataaa gtaccgttcc accacgtcta
     8101 catcaacgcc atcgtgcgcg acgcagaagg ccagaagatg tcgaagtcca agggcaatac
     8161 ccttgatccg ctagacctga ttgacggtgt cacgctgaat gcgctgctcg aaaaatccac
     8221 cacgggcctg ctgcgcgatg agcacaaagg aaaaatcgag aagtatgtcc gcaatcacta
     8281 cccgaacggc atcccttcat tcggtgccga tgcgctgcgc ttcaccttcg cctccctggc
     8341 cggcttcgcg cgcacgctca acttcgacct caaccgctgt gagggctacc gcaatttctg
     8401 caacaagctg tggaacgcca ctcgtttcgt gctcatgaac tgcgagggca aggactgcgg
     8461 gcaggacgat aaggccgcag tggcgctgtc gacttgggac agatggatcg tcagccgcct
     8521 gcagcgcact gaagccgagg tggcgaaagg cttcgatgag tatcgcttcg acctcgccgc
     8581 ccaggcaatc tacgaattcg tctggaacga gtactgcgac tggtacgtgg agctcgcaaa
     8641 ggtacagctc gcgaacggct cagaggaaga gcaacacggc acgcgcagaa cactggtgcg
     8701 cgtgctggaa gcggcgttgc ggcttgcgca tccgattatt ccattcatca cggaggaact
     8761 gtggcagaaa gtcgcgccgc ttgcgggcaa gagcggcgaa accgtcatgc ttgctccata
     8821 cccgaagagc caacccgaaa aaattgacga agaggtagag cgcgaagtcg acgaagcaaa
     8881 ggacgttgtg aacgccggac taaaacagcg cagcgagatc aagctccagc cacaatatcg
     8941 agtgccgtac tacatgacgg gtagaccgac caacaccaca acagctgcgt tctcggccct
     9001 attgcggccg tcggaacttc acatcgtcaa cgaacttccc caatcggact ctccggtagc
     9061 ggtcgtcagg tcgtatcgcg tgatgccgta cattaagatc gatgttgcgg cagagattga
     9121 gcgcattaag aaggaaatcg cacgcattga ggacgaaatc gccaaggcca acgccaagct
     9181 cgccaatccg tccttcgtcg agcgtgcgcc cgctgagatc atcgcgcagg aaaaagaacg
     9241 ccgcacaaac ttcggggcaa cgctggagaa actcaccgcg caactgggaa aactcacata
     9301 aaagaactaa gcataatacc ttgcccgcat acgctggcaa tacgcttcga ggcgaggata
     9361 tttcagcgcg tgttgcttga acgcggaatc cagcggaacc agaataatgt tggcgagaaa
     9421 agcataagcg gtggcgtcga gcgaagtcgg cttttctccc atgaaaaaca gcttgtcacc
     9481 caggaaatcg gcgagcgcgg tgatgtcggt tttgccgatg tgatgaacct cctccgcccc
     9541 gtgccgtccc atgccttgtc cccacatctg cctggcaacg ccgcggcgcg ccagggcgga
     9601 aatcggcccg cgcagcaatg ccggcaccgg tttgaaaaac tccgctttga ttgcctccca
     9661 gccttgcggc tccatccagc gcgtataggc aagcgcccag tagagatttt cttccatcat
     9721 ccgcttcagc gccaccgctt gtgcgttctc aaccggagtg agttttgcat cgagcgcatc
     9781 gccgtaggtt gatttcaagt attcaatgat gaagcttgaa tcggcaatca ccttggaccc
     9841 gtcctcgatg taaggcagtt tccctttcgg cgccttgaaa tgactggcat tgggcgcgat
     9901 ttcatattgc aagcccgcca tgcgcaagta gttttccact ttcatgcaga aaggactggc
     9961 gttgggcagt ccaaaggcag gctggaattg atagagcttg atcatgcggg taagcgagcc
    10021 gaatcagcgc tttttcataa agaaaatgaa ttcgccgccg gcctcggctt gcgacagcag
    10081 ttcatttccg gtctgcctgg aaaacgcctg gaaatccttg accgagccgg ggtcggtggc
    10141 aagtactttc aacacttgcc ccgaagtcat ttcagccagc gttttcttgg tgcgcaaaat
    10201 cggcaaaggg caattcaagc cgcgggtgtc gatttgcctg tcgaagttca tgccgcaact
    10261 cccgtgagcg gcagcccgtt tctcacccag gtgttgaccc cgccttgcag gtgatacacc
    10321 ttgttgaaac ccttgcctgc aaaaaatgtg caggcctgcg ccgagcgcgc gccggactga
    10381 cagtaaaaca cagtgggcgt ttccacgtcc cattccgccg ccttgggcgg aagtaaatgc
    10441 agcggaatgt gaatcgcgcc tttgatcatg ccgcgcgcta cttcagcatc tccgcggacg
    10501 tcaaccagcc gcaaattccc gcttgactgc atttcctgca agcgggccgc gtcaatttcc
    10561 tgaatgcttt gcatgggaag aatataacaa acttcaagtg cctcagaaaa tgctgatttt
    10621 gcgatctaaa agcagttgcg aaagccgcgt gttggcggcg gcgaggcggt cgaccaggat
    10681 ttgcaggaat gtacgatcaa actgggcgcg gcagttttcc gaggcctgtg ccagcgaatc
    10741 cgcggcgatt tcaatcgcgg tggtgtcatt gactgtcaca atgctggctg tgcgcttgaa
    10801 ccgggccttg cccaaataaa ccatttcgcc gaagcattcc ccggcttgca acacgttgag
    10861 gagcttgccg tccttgctca cgttcacttc gccggaagcc aggatgaaaa acgacttgcc
    10921 gacttcgccc tccttgagca gcgtgatgcc cggcttgtac ttgtgccagt tggtgatgcg
    10981 aagcacctcc cacagctcgg aatcattgaa atgcgcgaag aatgagagtt tgcgcagggt
    11041 gtcaaagcgc tcgccctcgg aaaagtcctc tttgggctgt tccagcgtgt tgtagacgtc
    11101 gatcagcgcc tgcgcgaaat cttcccaggt ttgatagcgc gtcaccagat ccttgcgaag
    11161 ggcggttttg acgattacat ccagttggac cggtatttcc ggacggtgca ggctgggcgg
    11221 cggcggatcg acgttgatga tctggtacac catgctgtag ttgttggtgg cagaaaacgg
    11281 taacttcccg gtcaacaact ggtacatcac cacgcccagc gaaaaaatgt cggtctggtg
    11341 attgagcggc atctcgcgca cctgctgcgg cgacatgtag gccggtgagc cgatgccgac
    11401 gatttgcgtg gtgtcggcgc cttcggtgag cgccgtgccg aaatcggaaa tcttgatgtc
    11461 caagtccgag gtcaataaaa tgttcgccgg cttgatgtcg cgatggatga tgccgtagcg
    11521 atgggcgaac gccagtgcgc ggctggcctt gaaaatgatt tcgatgacct tgtctaccgg
    11581 cagcaggttt tcgaccttgc agtatttttc cagcgtgccg cccgcaacgt attccatgac
    11641 gatgtagctc aaatcatcct ctaccaccgc atcgtaaatg gcgacgatgt gcggatgcga
    11701 gagcttgccc actagcgacg cttctgtcag gaagagtttt ttatagcggc gcccctgctg
    11761 cttgtcgcgc agcgcttttg catccaccag tttgatcgcg acctggcggt tagcaaaagg
    11821 gtcattggcg agatagaccg cgctggtcgc ccccttcccc agttccttga cgatttcgta
    11881 tttgccgatt ttgtccatga ccaagccgca ttacagcgcg gcaaatcaat tccgcataca
    11941 atacaggaaa cctgtgtttc agaagttcct tagtttatac gaagcaagca gctaatggac
    12001 atcccttcca ggacgattga acccccgcct agcgtatctt ttgccgaagc cttccgctac
    12061 tggctcatgc tcggcttcat cagcttcggc ggccccgcgg ggcaaatcgc cgtcatgcac
    12121 catgagctag tggaaaagcg gcgctggatt tccgaacggc gctttctgca cgcgcttaac
    12181 tattgcatgc tgctgcccgg ccccgaggcc cagcagctcg cgacctatat cggctggctc
    12241 atgcacaaga cgccgggcgg gattgtcgcc ggcgtgctgt ttgttctgcc ttcgcttttt
    12301 atcctgatcg cgctgacctg gatctatctc gcattcggca acgtcccggt cgtggctgga
    12361 atcttttatg ggatcaagcc ggcggtgacg gcaatcgtgg tgttcgccgc ctaccgcatc
    12421 ggcgcgcgcg cgctgaaaaa cgccgcgctg tggacgctgg cagctgccgc gttcatcgcc
    12481 atcttcgcgc tgaaaatacc tttcccttac atcgtgctgg ccgccggcat catcggctat
    12541 ttcggcgggc gcttggcacc ggcgaaattc gccatcggcg gtggacatgg cgcttccgac
    12601 aaaagttttg gccccaccat cattgacgac catacaccga caccggaaca cgcgcgcctg
    12661 agctggccac gcttcgcttt ttacagcgtg gtcgggctag tgttgtgggg cggggcaata
    12721 gccatccttg cctcgctcta cggctgggaa ggcacgctca cgcaaatggg ctggtttttc
    12781 accaaggccg cgctgctcac tttcggtggc gcctatgcgg tgctgcctta cgtttaccag
    12841 ggcggcgtcg aatattacca gtggctcacc ccggcgcaaa tgatagacgg cctcgctctg
    12901 ggcgagacca cgccggggcc gctcatcatg gtggtggcgt ttatcggctt cgtcggcggc
    12961 tggagcaagg aattgttcgg cccggacctg tcgtttgtct cgggcgccat cgcggcaacc
    13021 gtggtaactt actttacttt cctgccgtgc ttcctgttta tcctgctcgg cggcccgttt
    13081 atcgaaacca cccacggcga catcaggttc accgcgccgc tcaccggcat taccgcggca
    13141 gtagtgggcg tggtgctgaa ccttgcgctg tttttcgcct accacgtgct gtggccgcaa
    13201 ggtttcgaca accggtttga atggctgtcg ctggggatcg ggctcgccgc cttcatcgcg
    13261 ctgacgcgct tcggcatcgg catcatcccg gttatcggct gctgcggcgc gaccgggctg
    13321 gcctatactt tgctccgatt ctggtcgatg cgctagctta ttgcttgcgc aactcgactt
    13381 cgagggcacg gaattccttg agccgcgctt caacggcgga gagctgataa aaatcgccgt
    13441 cgccgctttt ctgcgcgatc tgcaactgct cgaccgcggc ccgcagattg cccaagagat
    13501 aataggcttc ggcctgtgac ttgtgttgca gtaatttttt atcgagcgcg gcgtagctct
    13561 tcgcttgcag cagataaaga tgatagtcct cgggataaaa ctgcagctgg tttgaagcga
    13621 gattgagcgc gtccagcgcc tggttctttt gcaagaatac atcacagctg gcgtagacca
    13681 gcggccggta ttgcggataa tttttcagcg ccgcacggta gatggccgac gcctccgcgg
    13741 ttttgccgcc cgccgattta agctgtccag ccaggatttc aatcaagggg ctgggctgcg
    13801 cgattttgcg cacctgcgcg agttcctgct cggcgcgctc aaggttgcgc gcgcgcagca
    13861 acgcaattgt cagcccgaag cgctgcgccg cttcacttgt gtatttcttc tcctgcaggc
    13921 tgttttcgaa ataggcgatt gcgtttttcg gcgattcttc ctgggcgcgc aggcgggcgc
    13981 gcaggaactg gaattcctgg ctgtcgggga cctgccggta gtgcatgttc tgcacacggt
    14041 tctgtatatc agcaatccgt tcaaaggtga ccgggtgagt gcgcaagtag gatggtgcat
    14101 taccctcaac aaagcgcgtg gctttctgca ggcgctcgaa aaataccggc atggctcgta
    14161 cgtcaaaacc cgccttgtcc agagtctgta aaccgatgcg gtctgcttcc tgctcgtgtc
    14221 cgcgggtaaa attgagctgg ttttgcgcta cggctgcctg gctgccaatt attgccgctt
    14281 gcgagagctg cgagttggag cgtgccgcaa gaatggctat cgcgataccc gccagcgaag
    14341 tggcgatcga ttgcttttgc gtggcgagga tgcgcgcgat gtggtgctgg gtcacgtgcc
    14401 cgatttcgtg cgccagcacc gaggcgagct cggattcgct ctgcgtgagg agaatcaggc
    14461 cagtgttgac gccgatgaag ccgccgggca gtgaaaatgc gttgatctcg ctgtcatgga
    14521 tagcgaacag atcgaggctg gtcttcggtt cgtcgctgct ggaaatgaga cggtagccca
    14581 agtcgttgag a
//
LOCUS       DATIOW010000059         2539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1041106, whole genome shotgun sequence.
ACCESSION   DATIOW010000059 DATIOW010000000
VERSION     DATIOW010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041106"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VLL03_01780"
     CDS             complement(<1..466)
                     /locus_tag="VLL03_01780"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HSS46130.1"
                     /translation="MRIFNSYLRQHIFGSTLLVFAVLLALFALLDLIHELGDLGANYQ
                     LHHVLIYVLLSVPGHVYELFPIAALIGTLLALAQLVVHSEYTVMRVSGVSAQGIVFSL
                     LQTGVIFVVLTFVFGEFVTPVSERAAHQLRLKALKLVVAQEFRSGLWVKDEGS"
     gene            complement(463..1545)
                     /gene="lptF"
                     /locus_tag="VLL03_01785"
     CDS             complement(463..1545)
                     /gene="lptF"
                     /locus_tag="VLL03_01785"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HSS46131.1"
                     /translation="MIFRRALLREFADIALAVFVVMFAIIVVTQLVRYLDAAASGAVA
                     PEGVIALLGFRAIYYLPVLLALTLFISVLLSLTRSYRDSEMIVWFSSGISITAWIRPV
                     LTFAVPLVIIVAVLSLFLSPWAFGKGEQYRQQLDARDDVATPTPGVFRESKQADRVYF
                     VGSAAGEGDTVSDIFVSSVQNQQLGIMVAKNGYQETRENGDRFLVLLHGRRYDGPPGS
                     SEYKITDFDKYDLRIEPYEAKQEVPQGRATSTLDLLRAGTPASLAEFVWRAGLPLSAL
                     LLALLAIPLSFVNPRVGRSFNLILALAVYMVYSNSLSIARGLVAQQKISLTLGFWGVH
                     ILMLVILALLFYKRLSVMPLARFIRR"
     gene            1641..>2539
                     /locus_tag="VLL03_01790"
     CDS             1641..>2539
                     /locus_tag="VLL03_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HSS46132.1"
                     /translation="MEFSIKIGGLEKQRSACVVAGVFAPRKLTPAASLIDRVSGGYLS
                     GITRRGDMQGKLGSTLLLHSVPRIVSERVLLVGLGRVQEFNEQHYREAVRAAVRALNE
                     TGVPEAVLGLAELPVKKRDIAWKILQAVLVAMDSTYRFDRLKSKHDEEKKPLDKLILM
                     VERSELIRGEAALKQALAIAEGVKLTKDLGNLPPNICTPTYLAEQASQIAKKHNLKAE
                     VLEQKDMEKLGMGSLLSVARGSHQPAKLIVLHYSGAAKKQKPVVLVGKGVTFDTGGIS
                     IKPAAEMDEMKFDMCGAASVLGS"
BASE COUNT          623 a          711 c          744 g          461 t
ORIGIN      
        1 agcttccttc atcctttacc cataacccgg agcggaattc ctgcgcgacc accagtttta
       61 acgctttgag gcgcagttgg tgagcggcgc gttcgcttac cggggtgacg aattcgccga
      121 acacgaaggt cagcaccaca aaaatcacgc cggtctgcag caatgagaaa actatgccct
      181 gcgccgacac ccccgagacg cgcatcaccg tgtattcgga atgcaccacc agctgcgcca
      241 gcgcgagtag ggtgccgatc agcgccgcga tgggaaataa ttcatatacg tggccaggca
      301 cgctgagcag cacatagata aggacgtgat gcagctggta attggcgccc aggtcgccca
      361 gctcatgaat caggtcgagc agcgcaaaca gggcgagaag cacggcaaaa accagcaggg
      421 tactgccgaa aatgtgctgt ctgagatagc tgttgaatat cctcatcgcc tgataaaccg
      481 cgccagcggc atgaccgata gccgcttgta aaacagaaga gcgagtatca ccagcatgag
      541 aatatgcacg ccccagaaac ccaacgtcag actgattttc tgctgagcca cgagcccgcg
      601 ggcgatgctc aagctgttgc tgtaaaccat gtaaaccgcg agcgcgagaa ttagattgaa
      661 ggagcgccca acgcgaggat tgacaaagct caagggtatc gccagcagcg caagaagcaa
      721 tgcgcttaac ggcaggccgg cgcgccacac gaactctgcg aggctcgccg gagtgcctgc
      781 gcgcagtaaa tcgagtgtgg atgttgcgcg tccctgcggg acttcctgtt tcgcctcata
      841 aggctcaatg cgtaaatcgt atttgtcaaa atccgtgatt ttgtattcgg aggaaccagg
      901 agggccatcg tagcgtctgc cgtgcaacag taccaggaaa cggtcgccat tttcgcgcgt
      961 ttcctgatag ccgtttttcg ccaccatgat gcccaattgc tggttctgta ccgaactcac
     1021 aaaaatatcg gatacggtgt cgccttcacc agcggcgctg cctacgaaat agacgcgatc
     1081 cgcctgcttg gattcgcgga acactccggg cgtgggggtc gcgacgtcgt cgcgggcgtc
     1141 gagctgctgc cggtattgtt cacccttgcc gaacgcccac ggtgaaagaa acaggctcaa
     1201 cactgcgact atgatgacca gtggaacggc aaaggtgagc accgggcgga tccaggcggt
     1261 gatgctgatg ccggaactaa accagacgat catttcggaa tcccggtaac tgcgggtcag
     1321 gctcagcaac acggaaataa acagggtcaa cgccagcagc accggaagat agtagatcgc
     1381 tctgaagccg agcagggcga tcacgccctc gggagcgacc gccccgctcg cggcggcatc
     1441 caggtagcgc accagctgcg tgaccacgat gatggcgaac atcaccacaa acaccgctag
     1501 cgcaatgtca gcgaattcac gcagcaaagc ccggcggaaa atcatgattc ggctgcctgc
     1561 cttttttgac ttttcataaa aactctgtgg ataatgcagc gtaacttgtt tcagcggggt
     1621 tttttgaata aggagcaaac gtggaattta gcataaaaat tggcggcttg gaaaagcagc
     1681 gcagcgcctg cgtagtggca ggagtattcg cgccgcgcaa actgacgcct gcggcaagct
     1741 taatagaccg ggtttccggc ggctacctga gcggcattac gcgccgcggc gacatgcagg
     1801 gcaagctcgg ctccaccttg ctgctgcata gcgtgccgcg gattgtctcc gagagggtcc
     1861 tgctggtggg cttgggacgc gtacaggaat tcaatgagca gcactaccgt gaagcggtgc
     1921 gcgcagccgt cagagcgctc aacgaaacgg gcgtgccgga agccgtgctg ggtctcgcag
     1981 aactgccggt caagaagcgc gacatcgcct ggaaaatcct gcaagccgtt ttggtggcga
     2041 tggactctac ctaccgcttc gaccgtctga aaagcaagca cgatgaagaa aaaaaaccgc
     2101 tcgacaaact aatcttgatg gtggagcgca gcgagttaat tcggggcgaa gcggcgctca
     2161 agcaggcgct tgccatcgcc gagggggtaa agctcaccaa ggacctgggc aatttgccgc
     2221 ccaacatctg caccccaacc tatctcgccg aacaggcttc gcaaatcgcc aaaaagcaca
     2281 atctcaaggc cgaggtgctg gagcaaaaag acatggaaaa gctcggcatg ggttcgctgt
     2341 tgtcggtggc ccgcggcagc caccagccgg ccaagcttat cgtgctgcac tattcgggcg
     2401 cagcgaagaa gcaaaaaccg gtggtgctgg tcggcaaggg cgttaccttt gacaccggcg
     2461 gtatctcgat caaacccgcc gcggaaatgg atgaaatgaa attcgacatg tgcggcgcgg
     2521 cgagcgtgct gggctcaat
//
LOCUS       DATIOW010000060         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1447116, whole genome shotgun sequence.
ACCESSION   DATIOW010000060 DATIOW010000000
VERSION     DATIOW010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447116"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VLL03_01795"
     CDS             complement(<1..964)
                     /locus_tag="VLL03_01795"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSS46133.1"
                     /translation="MFLRLVADSFGRRPRRKLLTGAALALGMAVVTAALSVALDVGDR
                     LAKEFRSLGANLLITPQADSLPLEIGGVDFRPVNAGAYLPEGELGKLKTIFWRHTIMG
                     FAPILEAQAQYLNSQKTAGDPARYVNVELIGTWSNHTVHIPDGSTFSTGIEKTNPWWK
                     VQGNLFSEGQKECLVGITLAKRLGIGAGDKILLNITDPSKMVQIFTPYKVTGTVSTGS
                     SEDQAIVMPLADVRRLGHLPEVYRRLYVSALTKPEDAFAKRDPKTFTPAEYDRWFCTP
                     YISSIAFQIQQELPGTDVRVIRRVAEGEGQILTRVQLLLWLVTGV"
     gene            complement(1124..>2086)
                     /locus_tag="VLL03_01800"
     CDS             complement(1124..>2086)
                     /locus_tag="VLL03_01800"
                     /inference="COORDINATES: protein motif:HMM:NF021587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing protein"
                     /protein_id="HSS46134.1"
                     /translation="EAYAGRSESAAGWGIFLFVFLMVLREGAELALMLRAVELSSEGL
                     QTWIGTIAGIAAAVAVGLFFFKGTLRIPLHRFFSVTTVILMLVAFQLALTGLHELSEA
                     RWLPSSKAEMAVLGPIVRNEVFFFVFIFGAAILLILREWQAAAHAKIAQDALNDAEKR
                     LLEAQNRRQRRWMMAAATASLAVILVLTADFIYARANSAPPSARVVGAAGDIVRLPVS
                     EVQDGNMHLFTVSAGAQTMRFMVIKKPNGWGVALDACRICGAEGYRQDGQNVVCRHCA
                     SAIYVPSIGNQGGCNPIGVPSHVDGGDLVIDISALAQVSTEIPK"
BASE COUNT          468 a          649 c          550 g          419 t
ORIGIN      
        1 ccacgccggt cactagccac aaaagcagtt gaacgcgcgt gagaatctgg ccctcccctt
       61 ccgccacgcg gcggatcaca cgcacgtcgg ttcccggcaa ctcctgctga atttgaaacg
      121 caatcgagga aatgtaaggg gtgcaaaacc aacgatcgta ctccgcaggc gtaaatgtct
      181 ttgggtcgcg cttcgcaaac gcatcttccg gcttcgtcag cgcgctgaca tacagtcgac
      241 ggtacacttc cggcaaatgc ccaagccgtc gcacatctgc gagaggcatg acgatggcct
      301 ggtcttcaga agaaccggtc gacaccgttc cagtgacctt gtagggggtg aaaatctgaa
      361 ccatttttga aggatctgta atattgagga ggatcttgtc acccgcacct atgcccaatc
      421 gcttcgcgag cgtaattcct acaaggcatt ctttctgtcc ttctgaaaac aagttgcctt
      481 gaaccttcca ccatggattc gttttctcaa tcccggtgct aaaagtgctc ccgtcaggta
      541 tgtggaccgt gtgatttgac caggtaccga tcaactctac gttcacatag cgggcagggt
      601 ctccggctgt tttttgactg ttgagatatt gcgcctgggc ttccaagatc ggcgcgaaac
      661 ccattatggt gtggcgccag aaaatcgtct tgagctttcc gagttctcct tcgggcagat
      721 aagcgccggc gttaacgggt ctgaaatcta ccccgccgat ttccagcggc agcgaatccg
      781 cttgcggcgt gatcagcaga ttcgcgccca agctgcggaa ttctttcgcc aaccggtcgc
      841 cgacatcgag cgcgacactg agcgcggccg tgaccacagc catacccaga gccagcgccg
      901 ctccggtcag gagtttccgg cgcggacggc gtccaaaaga atcggcaaca agtcgaagaa
      961 acatagctgg ctaaacgtta tcagtcgtca gtcctcagtc cggaaaggca ggttcctgtt
     1021 ttttccccaa tgcaacgttt tagtacttaa tactgaatac ttatgactga caaccttccg
     1081 gagtgcccct gaactcttcc ctctctcaat cctgcgtttg tgcctattta ggaatctctg
     1141 tgctgacctg agcgagggcg gaaatatcta tcaccagatc gcctccatcc acgtgcgaag
     1201 gcacgccaat cggattgcag ccaccttgat tgccgattga gggaacataa atcgccgaag
     1261 cacagtgtcg gcaaaccaca ttctgcccgt cctggcggta gccctcggcc ccgcagatgc
     1321 ggcaggcgtc cagcgccacg ccccacccat tgggtttctt gatgaccatg aatcgcatcg
     1381 tctgggcgcc ggcgctcacg gtgaaaagat gcatgttgcc gtcctgcact tcactcaccg
     1441 gcaggcgcac gatgtccccg gcagcgccga ccacgcgagc cgaaggcggc gccgaattcg
     1501 ctcgcgcata aatgaaatcc gcggtgagca ccaggatcac ggcaagagac gcggtcgctg
     1561 cggccatcat ccagcggcgc tgccggcggt tctgcgcttc gaggagccgc ttttccgcgt
     1621 cgttcagggc gtcctgggcg atttttgcat gcgccgccgc ctgccattcg cgcaagatca
     1681 gcaagatggc cgcaccaaaa ataaatacga agaaaaagac ttcattgcgc acgatggggc
     1741 ccaaaacggc catctccgcc ttgctcgaag gcaaccagcg cgcctcgctc agctcgtgca
     1801 ggccggtaag cgccagctga aatgccacaa gcatcaagat aacggtggtt accgagaaaa
     1861 atcggtgcag tggaatgcgc agtgtgccct tgaaaaagaa aagccccacc gcgacggctg
     1921 ccgcaatccc cgcgatcgta ccgatccagg tttgcagccc ctcgcttgaa agttccaccg
     1981 cgcgcagcat caacgccagc tcggcgccct cgcgcagcac catcagaaac acaaaaagga
     2041 aaattcccca gcccgcggcg ctctccgagc gcccggcata cgcctc
//
LOCUS       DATIOW010000061        12618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1143419, whole genome shotgun sequence.
ACCESSION   DATIOW010000061 DATIOW010000000
VERSION     DATIOW010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12618
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143419"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VLL03_01805"
     CDS             <1..757
                     /locus_tag="VLL03_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSS46135.1"
                     /translation="VLNGMNMRIAKGQVVSIMGGSGCGKTTLLRLIGGQLRPTNGAVR
                     VAGRVIHKLDHDDLYRMRRRMGMLFQFGGLFTDLSVFDNVAFPLREHTDLAEALIRDL
                     VLMKLNAVGLRGARDLMPSAISGGMARRVALARAIALDPMLIMYDEPFAGLDPISLGV
                     IANLIRRLNDALGATSIVVTHDVQETLAIVDYIYFMSEGVVAAQGTPDEIRASKLPFV
                     HQFVHAEMDGPVPFHYLAKPYAEDIGLGGGGHG"
     gene            750..1541
                     /gene="mlaE"
                     /locus_tag="VLL03_01810"
     CDS             750..1541
                     /gene="mlaE"
                     /locus_tag="VLL03_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HSS46136.1"
                     /translation="MVKQVAEGLRGLGGWAVHSVWRLGHASRFLWVTLVHSGTSFKRF
                     HLITREVYFVGVYSLIIIVVSGLFVGFVLGLQGYNTLRKYASEEALGLLVALSLVREL
                     GPVVTALLFAGRAGTALTAEIGLMKATEQLSAMEMMAVDPIARVIAPRFWGGVISMPL
                     LASIFTIVGVFGGYIVGVVLIGVDEGAFWAQMQAGVDVFKDVLNGTIKSVVFGFAVTW
                     IALFAGYESVPTAEGVARATTRTVVISSLAVLALDFVLTALMLGG"
     gene            1544..2047
                     /gene="mlaD"
                     /locus_tag="VLL03_01815"
     CDS             1544..2047
                     /gene="mlaD"
                     /locus_tag="VLL03_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HSS46137.1"
                     /translation="MKRNIHGERRLTRQTLDLWVGLFVLGGILALLMLAFKVGNLSSF
                     SLADDSYVLYANFENVGGLKVRAPVKSAGVLVGRVAEIRFDNKDYVARVTLHLDKRYQ
                     FPKDTFASILTSGLLGEQYVGLDPGGDSQYLKPGDTLAKTQSAVVLEKLIGQFLFNKA
                     QEGGNSK"
     gene            2044..2685
                     /locus_tag="VLL03_01820"
     CDS             2044..2685
                     /locus_tag="VLL03_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSS46138.1"
                     /translation="MKILGKLLVALTFVGSSAARAADAAPDTLVRDTSKEVLEILKKD
                     KDIQAGDRKKILELVEDKILPHFDFVRITRLAVGKNWRAATPEQQQALVKEFRTLLVR
                     TYSTALGAYREQTYEVKPADVKGDDATVKVQINQPGGQPIMLDYSMEKTQQGWKVYDV
                     TVGGISLVTSYRSSFTNEIGRAGIDGLLQTLAEKNQKLESGEEKPANLQLQKK"
     gene            2682..2981
                     /locus_tag="VLL03_01825"
     CDS             2682..2981
                     /locus_tag="VLL03_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSS46139.1"
                     /translation="MIEREGHRLRVKGPITLSNITALLEAGLQQFDLELKEIDFSQVQ
                     HVDSSAVSMLLEWLRQAQSRKLQLRVVNMPDNMQSLAALYGVLELIPSSRLQNKR"
     gene            3040..3936
                     /locus_tag="VLL03_01830"
     CDS             3040..3936
                     /locus_tag="VLL03_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSS46140.1"
                     /translation="MPAIQVSEVHKSFGAVQALAGVSLEIAPGEFFALLGPNGAGKTT
                     LINILAGLARPDRGKISVMGHDVLVQYREARRALGVVPQELVFDPFFTVRETLTIQSG
                     YFGLKNNHDWIDEIIHNLDLTDKADTNMRALSGGMKRRVLVAQALVHKPPVIVLDEPT
                     AGVDVELRQGLWEFIRKLNRDSHTIVLTTHYLEEAEALCGRIAMLKQGKIVALDTTRN
                     LLESFSRRQVKLLLAPDVLPEALQPLLSTREGNSFILSLPDYTELQRVLTQLREEKIE
                     VREIELLQTDLEEVFMNIMRKN"
     gene            3936..4691
                     /locus_tag="VLL03_01835"
     CDS             3936..4691
                     /locus_tag="VLL03_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSS46141.1"
                     /translation="MCGFSTLFRKELLRFWKVSFQTILAPVVTALLYLLIFSHVLEEH
                     VRVYEGVSYTAFLIPGLAMMTVLQNAFANSSSSLIQSKITGNIIFILLPPLSHWEFFI
                     AYMLAAVVRGLVVGLGVFAVTLWFTSVPLKYPLWILLFALLGSGILGNMGIIAGIWAD
                     KFDQLAAFQNFIIIPLTFLSGVFYSIHSLPPLWQKVSHFNPFFYMIDGFRYGFFGVSD
                     ISPYSSFAVVAGCFLALSWFTLWLIKIGYKLRY"
     gene            4693..4926
                     /locus_tag="VLL03_01840"
     CDS             4693..4926
                     /locus_tag="VLL03_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HSS46142.1"
                     /translation="MTTPGKIKSYIQDNLPCERVEVQGDGQHFEAVIVSAAFRQKNRV
                     QRHQIVYRALGDRMREEIHALSIKTLTPEEWTN"
     gene            4914..6167
                     /gene="murA"
                     /locus_tag="VLL03_01845"
     CDS             4914..6167
                     /gene="murA"
                     /locus_tag="VLL03_01845"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HSS46143.1"
                     /translation="MDKLVIQGGAPLSGEVRISGAKNAALPILCASLLTREPLRIENV
                     PHLRDVTTTLNLLAQMGVGVSLHEKMTVELTAQKLANRVAPYELVKTMRASILVLGPT
                     LARAGEAHVSLPGGCAIGLRPVDLHIKGLQAMGAEMVIEHGYINARSKRLKGARIFMD
                     LVSVTGTENLMMAATLADGTTVIENAAREPEVVDLANCLNAMGACIRGAGSEVISIEG
                     AKELHGARYRIMPDRIETGTFLLAAAATGGSVRLSETRADILDAVLDKLGEAGARVES
                     GEDWVELKMNGELKATNVRTAPYPGFPTDMQAQFMVLNCVAHGAATVTETIFENRFMH
                     VQELKRMGANIEVEGNTALVKGVPGLDGAKVMATDLRASASLVLAGMVARGETVVDRV
                     YHLDRGYECIEEKLSQLGARIRRAH"
     gene            6238..6891
                     /gene="hisG"
                     /locus_tag="VLL03_01850"
     CDS             6238..6891
                     /gene="hisG"
                     /locus_tag="VLL03_01850"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HSS46144.1"
                     /translation="MPGITLALSKGRIFDETVPLLKAAGICALDDPETSRKLILATNR
                     EDVRIVIARASDVPTYVQYGAADLGIAGKDVLDEHGGAGLYQPLDLNIGCCRMMVAVP
                     QEFDYESAVRQGARLKIATKYLQTAREHFAAKGMHVDLIKLYGSMELAPLTGLADAIV
                     DLVSTGNTLKANHLKAVEEIAPISARLIVNQAALKLKREAIKLLIDALGKAAANGKT"
     gene            6878..8176
                     /gene="hisD"
                     /locus_tag="VLL03_01855"
     CDS             6878..8176
                     /gene="hisD"
                     /locus_tag="VLL03_01855"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HSS46145.1"
                     /translation="MAKLRRLSTRSRNFEARFKALLAVEAALDGKVDATVAKILAEVK
                     KRGDAALLDYTRRFDRLKAKTVRELELPRARLAAALLTLPNPQRQAWEEAATRIRAYH
                     KKQLAQSWSYRESDGTRLGQQVTPLDRVGMYVPGGKAAYPSTVLMNAIPAKLAGVREL
                     IMTVPMPNGESNDLVLAAAAIAGVDRVFTIGGAQAIGALAYGTRSIPQVDKIVGPGNA
                     YVAAAKRRVFGVVGIDMIAGPSEILVICDGKTSPDWVAMDLFSQAEHDEMAQAILLCP
                     DRKFIEAVAASVKRLLPAMPRMKVIRASLEKRGALIEVKDLDEACEIANRVAPEHLEL
                     SVENAQGWAAKIKNAGAIFIGRYSSEALGDYCAGPNHVLPTSRSARFSSVLGVYDFQK
                     RSSLIEVSREGAVKLGKIAAELAAGEGLPAHAQSAQYRMK"
     gene            8192..9265
                     /gene="hisC"
                     /locus_tag="VLL03_01860"
     CDS             8192..9265
                     /gene="hisC"
                     /locus_tag="VLL03_01860"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HSS46146.1"
                     /translation="MAKSPDELIRDEIRTLRAYHAPESVGLVKLDAMENPHALPPALQ
                     IEMAQLASRVAINRYPDPQATGLQSQLRKVLQVDDSIDILLGNGSDELIQIVALAVAK
                     PGAVLLSVEPGFVMFRILATCAGLRYVGVPLKSDFALDAAAVIRAIKRHPPAVIFLAY
                     PNNPTGNLFDARAVTRVIKAAPGLVVVDEAYHAFAGASFIPRLADHRNLLVMRTFSKL
                     GMAGLRLGFLLGRAEWLAQLDKLRLPYNVNALTQAVAEKLLQHGAVFLEQAQQIKAER
                     EKMHAELVALTGIKAYRSDANFILFRVNRADEVFKGLIQRGILVKNLNCSHPLLENCL
                     RVTVGTAEQNQQFMQALKASLVN"
     gene            9300..9887
                     /gene="hisB"
                     /locus_tag="VLL03_01865"
     CDS             9300..9887
                     /gene="hisB"
                     /locus_tag="VLL03_01865"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HSS46147.1"
                     /translation="MRKAQVTRNTLETKISVKLNLDGSGKSKLATGVAFLDHMLDQVA
                     RHGLLDLEIAAKGDLRIDAHHTVEDIGITFGQAFAKALGDKKGVRRFGHAYVPLDEAL
                     SRVVVDLSGRPGIDYNLKFTRARIGEFDVDLVREFFQGFVNHALVTLHVDNLRGKNAH
                     HQCETVFKAFGRALRMAAEVDPRIKGVLPSTKGSL"
     gene            9901..10539
                     /gene="hisH"
                     /locus_tag="VLL03_01870"
     CDS             9901..10539
                     /gene="hisH"
                     /locus_tag="VLL03_01870"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HSS46148.1"
                     /translation="MIDIAVVDYGMGNLRSVAKALEHVAPEAKISVTDSPRQIREAKR
                     VVFPGQGAMPDCMREMDARGLREPVLEAAETKPFLGICIGLQMLFDWSEEGGVAGLGV
                     LPGKVRRFPREAMVDQGGAKLKVPHMGWNRVEQQQSHPLWNSIETGSRFYFVHSYYVE
                     ADKLQQVTGVSRYPFAFACAVARENIFAVQFHPEKSQAAGLQLLANFINWNS"
     gene            10643..11395
                     /gene="hisA"
                     /locus_tag="VLL03_01875"
     CDS             10643..11395
                     /gene="hisA"
                     /locus_tag="VLL03_01875"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HSS46149.1"
                     /translation="MLIIPAIDLKDGQCVRLEQGSMQKTTVFSDDPASQAKKWAEQGA
                     RRLHIVDLNGAFAGKPKNETEIKQIIAALGDKIPVQLGGGIRDLETVERYLDDGISYI
                     IIGTAAVKTPGFLHEACTAFPGHIMVSLDAKGGRVAVDGWSKMTGHDVVDLAKKFEDY
                     GVEAVIYTDIGRDGMLNGVNIEATVSLARELTTPVIASGGITSLDDVKALCKVEAEGI
                     MGAITGRAIYQGTLNFAEAQKLADKLSSKAKL"
     gene            11494..12264
                     /gene="hisF"
                     /locus_tag="VLL03_01880"
     CDS             11494..12264
                     /gene="hisF"
                     /locus_tag="VLL03_01880"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012362933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HSS46150.1"
                     /translation="MGLAKRIIPCLDVTGGRVVKGVNFVGLRDAGDPVEIARRYDEQG
                     ADELAFLDITASSDSRGLIFPMIEAVASQVFIPLTVGGGVRSVADVRSLLNAGADKVS
                     INTAAVENPQLVADASSRFGSQCIVVAVDAKQVSDGANPSWEVYTHGGRRARGLNAVE
                     WARRMQALGAGEILLTSMDRDGTRNGFDLALTRAICDAVDTPVIASGGVGNLQHLVEG
                     VLQGGADAVLAASIFHYGEFTVREAKEYMARHHIEVRL"
     gene            12277..>12618
                     /gene="hisI"
                     /locus_tag="VLL03_01885"
     CDS             12277..>12618
                     /gene="hisI"
                     /locus_tag="VLL03_01885"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HSS46151.1"
                     /translation="MTDAWLNQVNWTQDGLVPVVVQETGSSKVLMFAWMNREALQRTV
                     KSGEAVYWSRSRKKLWRKGEGSGHVQKVKEIRLDCDQDVLLLTVEQVGGIACHTGRHN
                     CFFHKLHDGKWV"
BASE COUNT         2609 a         3439 c         3967 g         2603 t
ORIGIN      
        1 cgtcctgaac ggtatgaata tgcgcatcgc caaaggccag gtagtctcca tcatgggcgg
       61 cagcggctgc ggcaagacta cgctgctgcg gctgattggc ggccagctcc ggcctacgaa
      121 cggcgcggtg agggttgcgg gcagggtgat acacaagctc gaccacgacg acctgtaccg
      181 catgcgccgg cgcatgggca tgctgttcca attcggcggg ctgttcaccg atttgtcggt
      241 gtttgacaat gtcgcttttc ccttgcgtga gcacacagat ttggcggaag cgttgattcg
      301 cgatttggtg ttgatgaagc tgaatgccgt gggactgcgc ggcgcgcgcg acctcatgcc
      361 gtcggcgatt tcgggcggca tggcgcggcg cgtggcgctg gcgagagcca ttgcgcttga
      421 cccgatgctc atcatgtatg acgaaccgtt tgccgggctc gatccgattt cactcggcgt
      481 gatcgccaat ctcatccggc gcctgaatga tgcgctcggc gccacctcga ttgtggtgac
      541 ccacgacgtt caggaaacac ttgccatcgt ggactatatc tactttatgt cggaaggggt
      601 agtggcggcg caaggcacac ccgacgaaat ccgcgcctcg aaactaccct tcgtgcatca
      661 gtttgtgcat gcggaaatgg acggaccggt gccgttccat tatttggcca agccgtacgc
      721 cgaggatatc ggactcgggg gaggcggcca tggttaagca ggttgctgaa ggtttgcgcg
      781 gactgggcgg ctgggcggtg cacagcgtct ggcgcctggg ccatgccagc cgctttttat
      841 gggtcacgct cgttcactcg gggaccagtt tcaagcgttt ccacctgatc acccgggaag
      901 tttatttcgt cggcgtgtat tcgctgataa ttatcgtggt ctcgggcctg ttcgtgggtt
      961 tcgtgctggg gcttcagggt tacaatacct tgcgaaaata cgcctccgag gaagctctgg
     1021 gcctgttagt ggccctgtca ttggtgcgcg agttgggtcc ggtggtgacg gcactcctgt
     1081 ttgccggccg cgccggcacc gcgcttaccg cggagatcgg cctgatgaag gcgaccgagc
     1141 agctttccgc gatggagatg atggcagtgg atccgattgc acgggtgatc gcgccgcgct
     1201 tttggggcgg cgtgatttcc atgccacttt tggcctcgat cttcactatt gtcggcgtgt
     1261 tcggcggcta tatcgtcgga gtagtgctga tcggggtgga cgaaggcgcg ttctgggcgc
     1321 aaatgcaggc cggtgtggat gtgttcaagg acgtgctgaa cggcacgatc aaaagcgtgg
     1381 tgttcggctt cgcggtgacc tggatcgccc tgttcgcggg ctacgagagc gttcccaccg
     1441 cggagggcgt ggcgcgcgcc accacccgca ccgtagtgat ctcgtcgctg gcggtgctgg
     1501 cgctggattt cgtattgacg gccttgatgc ttggagggta gtgatgaaac gcaatattca
     1561 cggtgaaagg cgacttacac ggcaaacgct cgatttgtgg gtcggcctgt tcgtcctggg
     1621 cggtatcctg gcgctgctca tgctggcgtt caaagtcggc aacttgagca gcttcagcct
     1681 ggctgacgac agttatgtgt tgtatgcgaa tttcgagaac gttggcggct tgaaagtgcg
     1741 cgcgccggtc aaaagcgccg gcgtgctggt cggacgggtc gccgaaatcc gttttgataa
     1801 caaggactac gtggccagag taaccttgca cctcgacaaa cgttatcagt ttcctaaaga
     1861 cacctttgcc agcattttga cctccggttt gcttggcgag caatatgtgg gactggaccc
     1921 gggcggggac agccagtatc tcaagcctgg agacaccctt gccaagaccc agtcggcggt
     1981 ggtgctggaa aaattgatcg gccagttttt gttcaataag gcgcaagaag gcggaaacag
     2041 caaatgaaaa ttctcggcaa gcttttggtg gccctgacgt ttgtgggaag cagtgcggcg
     2101 cgcgcggcgg atgcggctcc ggatacgctg gtgcgcgaca cgtccaaaga agtgctggag
     2161 atccttaaaa aggacaagga tatccaggcc ggggaccgca agaaaattct cgaactggtc
     2221 gaagacaaaa tcctgccgca tttcgatttc gtgcgcatca cccggctggc ggtgggcaaa
     2281 aactggcgcg ccgccacgcc ggaacagcag caggcactgg tgaaggaatt ccgcacgctg
     2341 ctggtgcgca cctattccac cgcgctcggg gcctacaggg agcagaccta tgaagtgaaa
     2401 cccgccgatg tcaagggcga tgatgcgaca gtgaaggtgc agattaacca gcccggcggg
     2461 cagccgatca tgcttgacta cagcatggaa aagacgcagc aaggctggaa ggtttacgac
     2521 gtgacggtcg gcggcatcag cctggtgacc agttaccgca gctcgttcac caacgaaatc
     2581 ggccgcgccg gcatcgacgg actgctccag acgctggcgg aaaagaacca gaagctggaa
     2641 agtggcgagg aaaaacccgc caatctgcag ttgcaaaaaa aatgatcgag cgcgaaggcc
     2701 acaggctgcg agtcaagggc ccgattactc tgagcaatat caccgcgctt ttggaagcgg
     2761 ggctgcagca attcgattta gaactgaagg aaatcgattt cagccaggtg cagcacgtcg
     2821 attcgtccgc agtaagcatg ctgctggaat ggctgcgtca ggcgcaaagc cgcaagctgc
     2881 aactgcgcgt cgtcaatatg ccggataata tgcagagctt ggccgcgctt tacggcgtgc
     2941 tcgaactcat cccgtcctcg cgcctgcaaa ataagcgata atagcctgcc gcaggattcg
     3001 cgagcgacct tgtcgccgtc cttgaacgtt tgttccatga tccccgccat tcaagtcagt
     3061 gaagtgcata agagcttcgg cgcggtgcaa gcgctcgccg gagtgagcct ggagattgcg
     3121 ccaggcgagt ttttcgctct gctgggcccc aacggcgcgg ggaagaccac cttgatcaac
     3181 atcctggcgg ggctggcgcg ccccgaccgt ggaaagattt cggtgatggg gcatgacgtg
     3241 cttgtgcagt atcgcgaggc acggcgcgcg cttggggtgg tgccgcagga actggtgttc
     3301 gatccgtttt tcacggtgcg ggagacgctc accatccagt cgggttattt tggcctcaag
     3361 aacaaccacg actggattga cgagatcatt cacaacctcg atctcaccga caaggcggat
     3421 accaacatgc gcgcgctctc cggcggcatg aagcggcgcg tactggtggc gcaggcgctg
     3481 gtgcataagc cgccggtcat cgtgctcgat gagccgaccg cgggagtgga cgtggaattg
     3541 cgccagggac tgtgggaatt catccgcaag ctcaaccgtg acagccacac aatcgtactg
     3601 accacgcatt acctagaaga ggcggaggcg ttgtgcgggc gcattgccat gttgaagcag
     3661 ggcaaaatcg tcgcgctcga taccactcgg aatctgctgg agagcttttc ccggcgccag
     3721 gtgaagctgc tgcttgcgcc ggatgtgttg cctgaagcct tgcaaccgct gttgagcacc
     3781 cgtgaaggca acagcttcat tttgtcgctt cccgattaca ctgagctgca gcgggtgctc
     3841 acgcagctca gggaagaaaa aatcgaagtg cgggaaatcg agctgctgca aaccgacctc
     3901 gaggaagttt ttatgaatat catgagaaag aactgatgtg cggctttagc acattgtttc
     3961 gcaaggaatt attacgcttc tggaaagtga gcttccagac catcctcgcg ccggtggtga
     4021 cagcgctcct gtatctgctg attttttcgc atgtactgga agagcatgtc cgcgtgtatg
     4081 aaggcgtgag ctataccgcg ttcctgatcc ccggcctggc gatgatgacg gtgctgcaga
     4141 atgcgtttgc caacagttcc tccagcctga ttcagtcgaa aatcaccggc aacatcattt
     4201 tcatcctgct gccgccgctt tcccattggg agtttttcat cgcctacatg ctcgccgccg
     4261 tcgtgcgcgg gctggtagtg ggtttgggcg tgtttgcggt tacgctgtgg tttaccagcg
     4321 tgccactcaa atacccgctg tggatcctgc tgttcgccct gctggggagc ggcattctcg
     4381 gcaacatggg cattatcgcc ggcatctggg ccgacaagtt cgaccagttg gcggcgtttc
     4441 agaatttcat catcattcca ctgacattcc tctccggtgt gttttattcg attcattccc
     4501 tgccgccgtt atggcagaag gtttcgcatt tcaatccgtt tttctacatg atcgacggct
     4561 tccgctacgg ttttttcggc gtctccgaca tttcgcccta ttcgagcttc gcagttgtcg
     4621 cgggctgttt cctcgcttta tcatggttca ctctttggct gatcaaaatc ggctacaagc
     4681 tgcgttacta aaatgaccac gcctgggaag atcaaaagct acattcaaga caacttgccg
     4741 tgcgaacggg tggaagtgca gggcgacggc cagcacttcg aggcggtgat tgtgagcgcg
     4801 gcattccgcc agaagaaccg ggtgcagcgc caccagattg tttaccgcgc gctgggcgat
     4861 cgcatgcgcg aggaaatcca tgcgctctcg ataaagacgc tgacgccgga agaatggaca
     4921 aactagtcat tcagggcggc gcgccgcttt ccggtgaggt gcgcatttcg ggcgccaaaa
     4981 atgccgcgct gccgattctc tgcgcgagcc tgctcacccg cgagccgttg cgaatcgaaa
     5041 atgtgccgca tctgcgcgat gtcaccacca cactgaattt gctggcgcag atgggagtag
     5101 gagtatcgct gcacgagaaa atgacggtgg aactcaccgc ccaaaaactt gctaatcggg
     5161 tcgcgccgta tgagctggtg aagaccatgc gcgcgtccat actggtgttg ggcccgacgc
     5221 tcgctcgcgc gggagaggca cacgtgagtc tccccggcgg ctgcgccatt gggttgcggc
     5281 cggtggactt gcacatcaag ggcctgcagg cgatgggggc ggaaatggtc atcgagcatg
     5341 gctacatcaa tgcgcgttcc aaacgcttga agggcgcgcg catattcatg gatctggtgt
     5401 cagtcaccgg caccgagaat ttgatgatgg cggcgacgct ggcggacggc acgaccgtga
     5461 tcgaaaacgc ggcgcgcgag ccagaggtgg tggatctggc gaattgtctc aacgccatgg
     5521 gcgcttgcat tcgcggcgcg ggaagcgagg tgatcagcat cgagggcgcc aaagaattgc
     5581 acggggcgcg ctaccgcatc atgcccgacc gcattgaaac cgggactttt ctgctggcgg
     5641 cggcggcaac cggaggcagc gtccggctga gcgaaactag agcggacatt ctcgatgcgg
     5701 tattggacaa gctgggcgag gccggcgcac gtgtggaatc aggtgaagac tgggtggaac
     5761 tgaaaatgaa tggtgaatta aaggcaacca atgtgcgcac cgcgccttat ccgggtttcc
     5821 ccaccgatat gcaggcgcag ttcatggtgt tgaactgcgt ggcgcatggt gccgccactg
     5881 tcactgaaac gatttttgaa aaccgcttca tgcacgtgca ggaactcaaa cgcatgggcg
     5941 caaatattga agtggaaggc aataccgcgc tggtgaaggg agtgcccggg ctcgatggcg
     6001 cgaaagtgat ggcgaccgac ctgcgcgcct cagcaagcct ggtgctggca ggaatggtcg
     6061 cccgcggtga aactgtcgtc gaccgcgtct atcatctcga ccgcggctat gagtgtatag
     6121 aagagaagct ctcgcagctc ggcgcgcgta tccgccgcgc acattgattt tcaacgaaaa
     6181 actcctgcgc tcggccacgt ctctgcggta aaatgcacct ttttaataga ggccgccgtg
     6241 cccggcatca ccctcgcctt gtccaaaggc cgcatcttcg atgaaaccgt gcctcttctg
     6301 aaagcggcgg ggatttgcgc gctcgacgat cctgaaactt cgcgcaagct gatcctggcg
     6361 accaaccgcg aagacgtccg catcgtcatc gcacgcgcct ccgacgtgcc gacctatgtg
     6421 cagtacggcg cggcggatct gggcattgcc ggcaaagatg tgctggatga gcatggcggg
     6481 gcgggactat accagccgct ggatttgaac atcgggtgct gccgcatgat ggtggcggtg
     6541 ccgcaggagt tcgattacga aagcgcggtg cgacagggcg cgcgactcaa gattgcgacc
     6601 aaatacctgc agacggcgcg cgagcacttc gccgccaagg gaatgcatgt tgatttgata
     6661 aagctctacg gctcgatgga gcttgcgccg cttaccggcc tggccgacgc gatcgtggat
     6721 ttagtgagca ccggcaacac gctgaaagcg aaccacctga aagcggtgga agaaatcgcg
     6781 cccatctcgg cgcggctcat cgtcaatcag gcggcgctga aactcaagcg cgaagcgatc
     6841 aagctgctga ttgacgcgtt aggaaaggcg gcggcgaatg gcaaaactta gacgtctttc
     6901 aacccgcagc cgcaatttcg aggcgcgatt caaggcgctg cttgccgttg aagcggccct
     6961 ggatggcaaa gtggatgcca cagtggccaa aatccttgcg gaggtaaaga agcgcggcga
     7021 tgcggcgctt cttgattaca cccggcgttt tgaccggctc aaagcgaaaa cggtgcgcga
     7081 gctggaactg ccgcgggcgc gcctggccgc ggctttgctc acccttccta atcctcaacg
     7141 gcaggcatgg gaagaggcgg cgacccgtat ccgcgcttat cacaaaaagc agcttgccca
     7201 atcctggagc tatcgcgaaa gcgacggcac gcggcttggc cagcaggtca cgccgctgga
     7261 tcgcgtcggc atgtatgtcc ccggcggcaa ggcggcctat ccttccaccg tgctgatgaa
     7321 tgcgattccc gcgaaactgg cgggggtgcg cgagctcatc atgaccgtgc ccatgcccaa
     7381 cggcgagagt aacgatttgg tgctcgccgc cgcggccatc gccggcgtgg atcgcgtctt
     7441 caccatcggc ggcgcgcagg cgataggcgc gctggcttac ggcacccgga gcatcccgca
     7501 ggtggacaaa atcgtgggtc ccggcaacgc ctatgtcgct gcggccaagc gccgcgtatt
     7561 cggcgtggtg gggatcgaca tgatcgcggg cccttcggaa atcctggtga tttgcgacgg
     7621 caaaacctct cccgactggg tggcgatgga tttgttctcc caagccgagc acgacgaaat
     7681 ggcgcaggcg attctgcttt gccccgaccg aaagttcatt gaggcagtgg ccgcgagcgt
     7741 caagcggctg ctgccggcca tgccgcgcat gaaagtgatc cgcgcttcgt tggaaaagcg
     7801 cggcgcgctg atcgaagtaa aagatctgga tgaggcctgt gagatcgcca accgcgttgc
     7861 gcccgagcat ctggagcttt cggtggaaaa cgcgcaaggc tgggcggcca aaatcaagaa
     7921 tgccggtgcg atttttatcg ggcgctacag ttccgaggct ttgggcgatt actgcgctgg
     7981 gcccaatcac gtgctgccga cttcgcgcag cgcgcgcttc tcgtcggtcc tgggtgttta
     8041 cgatttccag aaacgctcca gtctgattga ggtgtccagg gagggcgcgg tcaagctcgg
     8101 caaaatcgcg gcggaactcg ccgctggcga aggcttaccc gcacacgctc aatccgccca
     8161 ataccggatg aaataactcc ggcggttcaa catggcgaaa tcccctgatg agttaatccg
     8221 cgacgagatt cgcacgttga gagcttatca cgcgccggag agcgtcggcc tggtgaaact
     8281 cgatgcgatg gagaacccgc atgctttgcc gccggcgctg caaatcgaaa tggcgcaact
     8341 ggcaagccgg gttgcgatta accgctatcc cgatccgcag gcaaccggct tgcaatcgca
     8401 gttgcgaaaa gtgttacagg tagatgatag cattgatatt cttctgggca acggctcgga
     8461 tgaactcatt caaatagttg ccctggcggt ggcgaaaccg ggagctgtat tgctgagtgt
     8521 cgagcccggc ttcgtgatgt tccgcatact ggccacttgt gctggcttgc gttacgtcgg
     8581 cgtgccgctt aaatccgatt tcgcgcttga tgccgctgcc gttatccggg ccatcaagcg
     8641 gcacccgccg gcggtgattt ttctcgctta tcccaataac ccgaccggta atctgtttga
     8701 tgcgagggcg gtaacacgcg tcattaaagc ggcgccgggg ctggtggtgg tggatgaggc
     8761 atatcatgcg tttgccggcg ccagcttcat accgcggctt gccgatcacc gtaatctgct
     8821 agtgatgcgc actttctcca aactgggcat ggcgggattg cggctgggat ttctgctcgg
     8881 tcgcgccgag tggctggccc aactcgataa actgcgtttg ccgtataatg tgaacgcgct
     8941 gactcaagcg gtggccgaaa agctgttgca gcatggtgcg gtttttttgg agcaggcaca
     9001 acagataaag gcggaacgcg aaaaaatgca tgccgaactc gtggcgctga cgggcatcaa
     9061 agcgtatcgc agcgacgcca atttcattct gttccgggta aaccgggcgg acgaggtgtt
     9121 taagggttta attcaacgcg gcatactggt taaaaatttg aactgcagcc atccgctgct
     9181 ggagaactgc ctgcgggtga cggtgggcac ggccgaacag aaccaacaat tcatgcaggc
     9241 gctcaaagcc agcctcgtca actgaatttc ttccctttct ctttaattta agatacgcca
     9301 tgcgcaaggc acaggtaacc cgcaatactc tggaaactaa aatcagcgta aagctcaacc
     9361 tggacggcag cggcaagtcc aagctcgcca ccggggtcgc gtttctcgac cacatgctgg
     9421 accaggtggc gcgccacggc ttactggatc tggaaatcgc tgcgaagggc gacttgcgca
     9481 ttgatgcgca ccacaccgtg gaggatatcg gtattacttt cggccaggcg tttgccaagg
     9541 cgctcggcga caagaaaggt gtgcgccgct tcggccacgc ttatgtgccg ttggatgagg
     9601 cgctctcccg cgtggtggtg gatttatcgg ggcggccggg aatcgattat aacctgaaat
     9661 tcacccgcgc ccgaatcggc gaattcgacg tggacctggt gcgcgagttt tttcagggct
     9721 ttgtcaatca cgcgttggtg acgttgcacg tcgacaatct gcgcggcaag aacgcgcacc
     9781 atcagtgcga gaccgtgttc aaggctttcg gccgggcgct gcgcatggcg gctgaagtcg
     9841 acccccgcat caaaggcgtg cttccttcta ctaaaggcag tctgtaggca acagcagtgc
     9901 atgattgata tcgcagtggt ggactacggc atgggcaatc tgcgctcggt ggcgaaagcg
     9961 ctggagcatg tcgcgcccga ggcgaagata agcgtaacgg attcccctcg gcagattcgt
    10021 gaagccaagc gcgtggtatt tcccggccag ggtgcgatgc ccgactgcat gcgtgaaatg
    10081 gatgcccggg gcttgcgcga gccggtgctg gaggcggcgg aaaccaagcc ctttctcggc
    10141 atctgtatcg gtttgcaaat gctgttcgat tggagcgagg aaggcggcgt tgcgggcctg
    10201 ggcgtgcttc ccggaaaagt gcggcgtttt ccgcgtgaag caatggtgga tcaaggcggc
    10261 gccaagctca aagtgccgca catgggctgg aaccgggtcg agcagcaaca gtctcacccg
    10321 ttgtggaata gcattgagac aggcagccgc ttttattttg tccacagcta ttatgttgaa
    10381 gccgataaac tgcaacaggt gacgggcgtg agccgctatc cgtttgcctt tgcctgcgcg
    10441 gtggcgcgcg aaaacatttt cgcggtgcaa ttccaccctg aaaaaagcca ggccgcgggt
    10501 ttgcagctgc tcgccaactt tataaactgg aatagttgat gcgttccaca caacgccggt
    10561 ttgtgtgtat catttaagcg tgaaatccgc agtgcggcca acctacgcct agttcacccc
    10621 gcttgatttt ccgttccaag caatgctgat cattcctgcg attgatttga aagacgggca
    10681 atgtgtgcgc ctcgagcaag gctcgatgca aaaaaccacg gtgttttccg atgacccggc
    10741 aagccaggcg aaaaaatggg ctgagcaggg cgcgcggcgc ctgcacatcg tggatttgaa
    10801 cggcgcattt gcagggaagc ccaagaacga aaccgagatc aagcaaatca ttgctgcgct
    10861 gggggataaa atcccggtgc agctgggtgg aggcatccgc gatctggaaa ccgtcgagcg
    10921 ctacctcgac gacggcattt cctacatcat cattggcacc gctgcggtga aaacccccgg
    10981 tttcctgcac gaggcctgca ccgcttttcc cggccacatc atggtcagtc tggacgccaa
    11041 gggcggcaga gtcgcagtgg acggctggtc gaaaatgacc ggccacgatg tggtggacct
    11101 cgccaagaag ttcgaggact acggcgtgga agcggtgatt tacaccgaca tcggccgtga
    11161 cggcatgcta aacggcgtca acatcgaggc cacggtcagc cttgcgcgcg aacttaccac
    11221 gccggtgatt gccagtggcg gcatcaccag cctcgacgat gtgaaggcgc tgtgcaaggt
    11281 cgaagccgaa ggcatcatgg gtgcgattac cggccgcgcc atctaccagg gcacgctcaa
    11341 tttcgccgaa gcgcagaagc tggccgacaa actcagctcg aaagcgaagc tttgaaccgc
    11401 ggagacgcag aggccctgag aatttcaagt aaaaggtttg ccctgacttt ggtttctctg
    11461 cgtcttcgcg cctctgcgca ggaaaattga tttatgggcc tcgcgaaacg tatcattccc
    11521 tgcctcgatg tcaccggcgg ccgcgtggtc aaaggcgtta atttcgtggg cttgcgcgat
    11581 gcgggcgatc cggtggaaat tgcgcgccgc tacgacgaac agggcgcgga cgagcttgct
    11641 tttctcgaca tcaccgcgag ttccgattcc cgcgggctga tatttcccat gatcgaagcg
    11701 gtggcgtcgc aggtgtttat cccgctcacc gtcggcggcg gggtgcgctc ggtggcggat
    11761 gtgaggagcc tattgaatgc gggagccgat aaagtgagca tcaacaccgc ggcggtggaa
    11821 aatccccagc tggtggcgga tgccagcagc cgcttcggct cgcaatgcat cgtcgtggcg
    11881 gtagacgcaa aacaggtcag cgatggcgcc aatccgagtt gggaagtcta cactcacggc
    11941 ggacgcaggg cgcggggact taatgcggta gaatgggcaa ggcgtatgca ggcgctgggc
    12001 gccggcgaga ttttgcttac cagcatggat cgcgacggca ctcgaaacgg ttttgatttg
    12061 gcgctcacgc gcgccatttg cgatgcggtc gatactccgg tgattgccag cggcggcgtg
    12121 ggcaatttgc aacatctggt ggaaggcgtg ctgcaaggcg gggcggatgc cgtgctcgcg
    12181 gcgagcattt tccactacgg cgaattcacg gtgcgcgagg ccaaggaata catggccagg
    12241 caccatatcg aagtcaggtt atgatgagat aggttcatga cggacgcctg gttaaaccaa
    12301 gtaaactgga cgcaggatgg tttggttccg gtagtggttc aggaaacagg cagcagtaag
    12361 gtgctgatgt ttgcctggat gaaccgtgaa gccctgcaac gcactgtaaa aagtggtgaa
    12421 gcggtgtact ggtcgcgctc gcgcaagaaa ctgtggcgca agggtgaggg atccggccat
    12481 gtgcaaaaag tgaaggaaat ccgcctcgat tgcgaccagg atgtgctgtt gctgacagtg
    12541 gaacaggtgg gcggcattgc ctgccatacc ggcagacaca attgtttttt tcacaaactg
    12601 catgacggca aatgggtg
//
LOCUS       DATIOW010000062         2164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_940782, whole genome shotgun sequence.
ACCESSION   DATIOW010000062 DATIOW010000000
VERSION     DATIOW010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940782"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VLL03_01890"
     CDS             complement(<1..620)
                     /locus_tag="VLL03_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HSS46152.1"
                     /translation="MLLGFPLLSLAIWLPILFGLLVLATGGDRNAPRARWLALAGALQ
                     GFLVTLPLYAKFDISMAEMQFVEMRPWIERFNAHYHLGVDGISLLFILLNSFMTLLVV
                     IASWRVIETRVAQYMAAFLIMSGLLNGVFSALDAVLFYLFFEAMLIPMFLIIGVWGGP
                     DRVYAAVKFFLYTLLGSLLMLVAFIYLYNASGGSFEILDYHDLRLSL"
     gene            complement(659..>2164)
                     /gene="nuoL"
                     /locus_tag="VLL03_01895"
     CDS             complement(659..>2164)
                     /gene="nuoL"
                     /locus_tag="VLL03_01895"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HSS46153.1"
                     /translation="SYLLIGFWYTRPSAIFANLKAFLGNRVGDFGFLLGIAGVLYYTG
                     SLDYAAVFQQAPQLANRTIEILPNEPWAVMTLICILLFSGAMGKSAQFPLHVWLPDSM
                     EGPTPISALIHAATMVTAGIFMVARMSPLYELSETALSVVLVIGAITALFMAFIAIVQ
                     NDIKRVVAYSTLSQLGYMTVALGASAYAAGIFHLMTHAFFKALLFLGAGSAIIALHHE
                     QDMRKMGGLWRYMPITYATVFIGSLAGAGIPPFSGFFSKDAIIEAVHLSRLPGAGFAY
                     FCVLSCVFVTALYTFRLLFMTFHGEPRMDEHLRGHLHESPPVVTVPLILLAIPSVYTG
                     WQYIEPMLFGGHFGRAIVVRPEHDVLGRLQEHFHGAFAFVLHGMTAAPFWLAVAGIGV
                     AYYLYMVRTDLPQKISERFRPVYNLLERKYGFDELYQWLFAGGARGVGGSLWQVGDVT
                     VIDGFFVNGTARVVRWTSTVIRHLQSGYIYHYAFSMIIGVFVLLTLWFSWP"
BASE COUNT          574 a          634 c          607 g          349 t
ORIGIN      
        1 agcgaaagcc gcaaatcgtg gtaatcgaga atttcaaagc tgccgccgga agcgttgtac
       61 aaataaataa acgccaccag catcagcagc gaacccagca gggtatacag gaaaaacttg
      121 actgcagcgt aaacgcggtc cgggccgccc catacgccga tgatgaggaa catcggaatc
      181 agcatggctt cgaagaacaa atagaacagc acggcgtcca acgctgaaaa cactccgttc
      241 aacaaacctg acatgatgag gaacgccgcc atgtattgcg ccacccgggt ttcaatgacg
      301 cgccaactgg cgatcaccac cagcagcgtc atgaagctgt tgaggaggat gaacaacaac
      361 gatatgccat ccacgcccag gtggtagtgc gcattgaagc gctcgatcca cggccgcatt
      421 tccacaaact gcatctcggc catagagata tcgaacttgg catagagcgg aagcgtgacc
      481 aggaaacctt gcagcgctcc agctagcgct agccagcgcg cgcgcggagc attgcggtcg
      541 ccacccgtgg caagcaccag gagcccgaat aggatgggca gccagatcgc caaactcaac
      601 agaggaaagc ctaacagcat gattgatccg ttttattatt ctgcagttgg tgtttatctc
      661 agggccagga aaaccataaa gtcagcaaca cgaatacgcc gataatcatg ctgaaggcgt
      721 agtggtaaat gtagcctgac tgaagatgac ggattacagt tgacgtccag cgcaccacgc
      781 gcgccgtgcc gttgacgaaa aaaccgtcaa tcaccgtcac atcgcccact tgccacaagc
      841 tcccgcccac gccgcgcgcg ccgcctgcga acagccactg gtagagctca tcaaaaccgt
      901 atttgcgctc gagtaggttg taaaccgggc ggaagcgctc gctgattttc tgtggcaaat
      961 cggtgcgcac catatacagg tagtaagcca cgccgattcc cgccacggcc agccagaatg
     1021 gtgcagcggt catcccgtgc agaacaaatg cgaacgcccc gtgaaaatgc tcttgcaagc
     1081 ggcccagcac gtcgtgctcg ggacgcacca cgatagcccg gccgaaatgc ccgccgaaca
     1141 acatcggctc gatatactgc cacccggtat ataccgaggg gatggcgagc agaatcagcg
     1201 gcacggtcac caccgggggt gattcatgca aatgcccgcg caaatgctca tccatgcgcg
     1261 gctcgccatg gaaagtcatg aacaacaggc ggaaggtata aagcgcggtc acgaacacgc
     1321 aactcaaaac acagaaatag gcaaagcctg cgcctggcag acgcgagaga tgcactgctt
     1381 cgataatggc atctttggaa aagaatccgg aaaacggcgg gatgcccgcg ccggcgagcg
     1441 acccgataaa gacggtggcg taggtaatgg gcatgtagcg ccacaacccg cccatcttgc
     1501 gcatgtcctg ctcgtgatgc agggcgataa tcgccgagcc cgcgcccagg aacagcagcg
     1561 ccttgaaaaa agcatgcgtc atgagatgga atatcccggc ggcgtaggcc gatgcgccca
     1621 gcgcgacggt catgtaaccc agctgcgaga gcgtggaata agccaccacg cgcttgatgt
     1681 cgttttgcac tatggcgatg aacgccatga acagagcggt aatcgcgcca atcaccagca
     1741 ccactgacag cgcggtttcc gacagctcgt aaagcggcga catgcgcgcc accatgaaga
     1801 tcccggcagt aaccatggtc gcggcatgga tcaacgcgga aatcggcgtt gggccttcca
     1861 tcgaatcggg cagccataca tgcagcggga attgcgcgga tttgcccatt gcgcccgaaa
     1921 acagcaggat gcaaatcagc gtcatcaccg cccacggctc gttcggcagg atttcaatcg
     1981 tccggttcgc gagctgcggt gcctgctgga ataccgcggc gtaatcgagc gaaccggtgt
     2041 agtacagcac cccggcgatg cccagcagaa agccgaagtc gccgacccga ttgcccagaa
     2101 aggccttgag gttggcaaaa attgcgctcg gacgcgtgta ccagaagccg atcagcaggt
     2161 aaga
//
LOCUS       DATIOW010000063         1739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1447989, whole genome shotgun sequence.
ACCESSION   DATIOW010000063 DATIOW010000000
VERSION     DATIOW010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447989"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1175)
                     /gene="glnL"
                     /locus_tag="VLL03_01900"
     CDS             complement(105..1175)
                     /gene="glnL"
                     /locus_tag="VLL03_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HSS46154.1"
                     /translation="MMPATFHGLELLATAVILLDEDLLMQYANPAAENLFELSTRNIG
                     GRSLEQVFDGADVLTTAVNYALDHNCSYIEHDLQLGIHGRPRLHLSCAVSALETSSAR
                     VLLEFRQMDQQLKIAREEHMLEQQQTNLELFRNLAHEIKNPLGGIRGAAQLLERELER
                     ASLHEYTQVIITEADRLQSLMGRLLTPHRLPRLTEINIHEVLEQMKTLLLAEYPQGIE
                     IQRDYDSSLPPLFGDREQLIQATLNIMRNAAQAMQGKGKIILRTRIARQTTLAKKRHR
                     LVIAVQIIDDGPGIPETIRERIFYPLVSGKEGGSGLGLTIAQTFVNQHHGIIECESRP
                     GHTCFTLLLPLPENGNAQPESA"
     gene            complement(1297..>1739)
                     /locus_tag="VLL03_01905"
     CDS             complement(1297..>1739)
                     /locus_tag="VLL03_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HSS46155.1"
                     /translation="SLLAIALLLICTPAPAEIYKQVDAEGRVTYSNIPSKGAQKLHLE
                     PLPSVPGYKAPAGTPGDFPKIDTDTQKGRDGKRRQLLESELEQEKKQLEDARQALTEG
                     EATRLGDERNNYQKYLDRVQRLKDTIAEHEKNVDALQQELAGLR"
BASE COUNT          332 a          506 c          491 g          410 t
ORIGIN      
        1 gggcgcggcg gcaaaagttt tgaacgcgat gccttcgcgc gcaagcgctt tttccagcac
       61 ccagcggatg gagcggtcgt cgtcaataat ccataccggt ttcatcaggc cgactccggt
      121 tgcgcgttgc cgttttcagg aagcggcaga agcagagtaa agcaggtgtg ccccggccgg
      181 ctttcgcatt cgataatacc atggtgctgg ttgacgaacg tctgggcgat ggtaagcccc
      241 agcccgctgc cgccttcctt gcccgagacc agcgggtaaa aaattctttc gcgtatcgtt
      301 tcgggtatgc ccggcccgtc gtcgatgatc tgcaccgcaa tgaccagccg gtggcgcttc
      361 ttcgccagcg tggtctgccg cgcaatgcgc gtgcgcaaaa tgattttgcc cttgccctgc
      421 atcgcctgcg ccgcattgcg catgatattc aatgtcgcct gtatcaactg ctcgcggtcg
      481 ccgaaaagcg gcggcagact gctgtcgtaa tcacgctgga tttcgatgcc ctgcggatat
      541 tccgcaagta acaacgtttt catctgctcc agcacttcgt ggatgttgat ctcggtcagc
      601 cgcggcaaac ggtgcggcgt cagcaggcgg cccatcaacg actgcaggcg gtcggcttcc
      661 gtgatgatga cctgggtgta ttcatgcagg ctggcgcgct caagttccct ttccagcaac
      721 tgcgccgcgc cgcggatgcc gcccagcgga ttcttgattt cgtgcgccag gttgcgaaaa
      781 agttcgaggt ttgtctgctg ctgctccagc atgtgctctt cccgtgcgat tttcagttgc
      841 tgatccattt gcctgaactc cagcagcacg cgcgccgatg atgtctccag cgcgctcact
      901 gcgcagctca ggtgcagccg gggacgccca tggataccaa gctgcaaatc gtgctcgatg
      961 taactgcaat tatggtcaag cgcatagttc acggcggtgg tgagcacgtc cgccccatca
     1021 aacacctgtt cgagcgaccg tcctccgatg ttacgggtgc tcaactcaaa caggttctcc
     1081 gcggcgggat tggcatattg catcagcaga tcctcatcga gaagaatcac tgcggtggca
     1141 agcaattcga gtccatgaaa agttgcgggc atcatgagca ctagccaagg cgggataaag
     1201 ttacagcaag aaccgcgcca tcataaagat aaaagcaccc gctagacgct tacgggttca
     1261 acggtaccgg attatggagg caaacttctg gattgcttat ctcaagcccg ccagttcctg
     1321 ctggagcgcg tcgacattct tttcgtgctc ggcgatcgta tccttgagcc tctgcactcg
     1381 gtccagatat ttctggtaat tgttccgctc gtcgcccagg cgcgtggctt cgccttcggt
     1441 gagcgcttgc ctggcgtcct caagttgctt tttttcctgc tccagctcgc tttcaagaag
     1501 ctggcggcgt tttccgtcac ggcctttttg cgtatcggtg tcgattttcg gaaaatcgcc
     1561 cggcgtaccg gctggcgctt tgtagccggg aacactgggc aagggctcca gatggagctt
     1621 ttgcgcgccc ttgcttggaa tattggaata agtgactcgg ccctctgcat ccacctgttt
     1681 gtagatttca gccggggccg gcgtacaaat cagcaacaac gcgatagcca atagagatc
//
LOCUS       DATIOW010000064         8741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1448519, whole genome shotgun sequence.
ACCESSION   DATIOW010000064 DATIOW010000000
VERSION     DATIOW010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8741
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448519"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            298..1626
                     /locus_tag="VLL03_01910"
                     /pseudo
     CDS             298..1626
                     /locus_tag="VLL03_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            1711..3639
                     /gene="selB"
                     /locus_tag="VLL03_01915"
     CDS             1711..3639
                     /gene="selB"
                     /locus_tag="VLL03_01915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HSS46156.1"
                     /translation="MIIGTAGHIDHGKSTLVKALTGVDPVRLQEEKARGITIDLGFAY
                     QPLSDGQVLGFVDVPGHEKFIHNMLAGATGIDYVMLVVAADDGPMPQTVEHLAILHLL
                     GLSRGIVALSKADLVSQERLAEVKAEIQSLLAPTPLAGAPILPVSAVSGEGMAELRQH
                     LLRAVYDSGVRNPGGHFRHAVDRCFTLQGVGVVVTGTVFSGEVKRGDKLFVSPSGIPV
                     RVRSIHAQNQQATSGHVGQRCALNLAGVEKNDIKRGNWILAEAVHAPAKRFDAHLNLL
                     NTEVKPLRHWTSVHVHVGACDVLGRVALLQANNLQPGDNALAQLVLDEPVGALHGDRF
                     VVRDQSAMRTMGGGIIIDPFPPQRGRRKAQRLEILSALESATAAQALQKLLALSPFGV
                     DWQKFVLTWNLNEAEAQTLSRQVAVTEVICAEKPLAFSSERWAGLKAQALQALQAYQK
                     RFPNQTGATTEQVWQGFPEKLQQGLLNVVIGQLIEEKKMVREGQLLRMPGLQASLSAA
                     DQKLSEKIIPLLQTAGLLPPKVRELSTQLRTDEKQLKLLLRRFSQMGKLRQVSEDYYF
                     APEVIARLAALTQKLAQQSPTGTITVGQFREGTAVYRNLAIPLLEFFDRSGFTVRVDD
                     GRRIRRDWKEVFGENKKA"
     gene            3726..3822
                     /locus_tag="VLL03_01920"
     tRNA            3726..3822
                     /locus_tag="VLL03_01920"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3761..3763,aa:Sec,seq:tca)
     gene            3843..5258
                     /gene="selA"
                     /locus_tag="VLL03_01925"
     CDS             3843..5258
                     /gene="selA"
                     /locus_tag="VLL03_01925"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HSS46157.1"
                     /translation="MSNKTAGNNLFALPSVDRVLNYAALELLIDEYGRASVLTAVRAV
                     LSDARKELLAGGNGAVFNEAWFGMHCRSQLEAQAQPSLRPVFNLTGTVLHTNLGRALL
                     PREAQEAALAVMGQPANLEYDLESGARGERDEHVEGLLCKLTGAEAVTVVNNNAAAVF
                     LVLNTLALKKEVPVSRGELIEIGGAFRIPDIMRRAGCKLVEVGTTNRTHLKDFEEALN
                     DQTAMLMKVHTSNYAIQGFTAAVAEKELGALAHKHNLPFMVDLGSGTLVNLKDFGLPA
                     EPTPQQALSHGADIVTFSGDKLLGGPQAGLIVGKKEVLKKIRKNPLKRALRVDKITLA
                     MLEAVLRLYRDPERLKERLPTFRLLTRPVEEIEALAKRLFPVLEKQFTGKGLVAIKPC
                     HSQIGSGSLPVEVLLSKCIAITPPQNERHPGTLLEKIAAAFRALPVPVIGRIEDDAFC
                     LDLRCLEDEQKFVNQLASLKI"
     gene            complement(5255..5686)
                     /locus_tag="VLL03_01930"
     CDS             complement(5255..5686)
                     /locus_tag="VLL03_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSS46158.1"
                     /translation="MKTPIFPTPQDAETAFYEAFERADLEAMMAVWAEDEEVICVHPS
                     GPRLVGHGQIRESWRQIFANGPRLRFHLVGQQYLRGMLLSVHSVYENIVVTGETAPRS
                     PIIATNIYLLTERGWRMLLHHACPAPATAKATGETPPTVLH"
     gene            complement(5683..6702)
                     /gene="waaF"
                     /locus_tag="VLL03_01935"
     CDS             complement(5683..6702)
                     /gene="waaF"
                     /locus_tag="VLL03_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HSS46159.1"
                     /translation="MTRILIVAPSWVGDAVLAQPLFKRLHERLPGIVLDVLAPLWVAP
                     LLSRMPEVNEVITNPFRHGELNLRDRRRLGHKLAARRYDQAIVLPNSWKSALIPFFAR
                     IPVRTGFVGEMRFGLLNDGRTLNKLQFPLMVERFALLGEERGTSLKRPLATPGLRLSA
                     NGREAVLKKLGLDARKPVVAFCPGAEYGPAKRWPPNYFAELARRLHGEGYQIWLVGSP
                     NDASICGEIQGLSGGDVVNLCGKTTLDEAIDLLACASLVVSNDSGLMHIAAALDKPLY
                     ALYGSSSPGFTPPLSDQARVIKLELPCSPCFQRVCPLKHFNCMMQLTPEKVLAKIDFK
                     KVEPS"
     gene            complement(6798..7004)
                     /locus_tag="VLL03_01940"
     CDS             complement(6798..7004)
                     /locus_tag="VLL03_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HSS46160.1"
                     /translation="MQEEKTANKERHIEVTAKDLPLHCPMPSMLLWNAHPRVFLPIEQ
                     TGEALCPYCGTRYTLKGGAIPEKH"
     gene            complement(7011..7931)
                     /locus_tag="VLL03_01945"
     CDS             complement(7011..7931)
                     /locus_tag="VLL03_01945"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HSS46161.1"
                     /translation="MSMADRDGHIWYDGKLVPWHDATTHVLTHSLHYGLAVFEGVRAY
                     KTVSGTAIFRLKEHTDRLFNSAHIYLMKMPFDKPTLLEAQKEVVRANKLEECYIRPIA
                     FYGPEKIGVSPKGAKVHVAIAAWPWGAYLGTEGLEKGIRVKTSSYVRHHVNVTMCRAK
                     YSGTYANSILANTEAVEHGYDEALLLDVDGFAAEGSGENLFIVKNGRLYEPELTSALI
                     GITRDAVIVLAHEAGYEVSAKRITRDDIYIADEAFFTGTAAEVTPIRELDGRAIGSGS
                     RGPITAKIQKMFFDCVNGKSDKHRDWLAPV"
     gene            8094..>8741
                     /locus_tag="VLL03_01950"
     CDS             8094..>8741
                     /locus_tag="VLL03_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46162.1"
                     /translation="MPDLAQANSNSAGILGRLTQSVISPSDSEDERLRKTLLLFVCGL
                     MNLAAGFWLMIYWSMGMRLPTTIPLGYQLVSALTLIIYLKTKNFAFFRFTQIGLLLFV
                     PFVIQWSIGNFISSSGIVLLALLAPVGAMVFQGVRESIPWFVAYVVLTAVSGVFDYYL
                     AAGVVSGVPMRIIPVFFVLNFTILSTIVYLLIRFFILKKDMFQTELGEKNRLLQVE"
BASE COUNT         1869 a         2354 c         2641 g         1877 t
ORIGIN      
        1 ttgggcgaaa tccctaatgg caagctggag cagattttga agcagttggc aggttagcag
       61 tttggattgt tttattaatt tcaatcaaca ggagaggagg tagccgtgtt acccgtgttc
      121 aagaaaaagc tgaccggcct ggcggcggcg atgacagtaa gcttcgcgct tgcgtcaccg
      181 ttggccatgg cggttagcgg caacaccatc acggtcgttg acccgtctcc cgttaactgg
      241 ctctggatta cctggaacac gatggaagag ctggtgcggg tggacaaaga cggccgtatg
      301 gtcaacaacc tggcggaaag ctatcggtgg gtggacgaca agacccttga attaaagctg
      361 cgcaagggcg tgacgttcca cgacggtgag cgactcactg gcccggtgtt ccgccgcagt
      421 ttcgacgagg ttcagcgctg ggaaaatccg cacccgccgg gcgcgttttt gaactacgcc
      481 aaaaccgcga aactcgacgt cgttaacgat tacaccatcc gtttcacctt ccccgagcca
      541 gacacttcga ctttcatgaa gctgcacggc atgcacgtgg gaagcacgcg cttttggaac
      601 gaactcggct ttatcgacaa gaagaccggc aaggccgaaa gccattggtg agtgattgac
      661 gcagccggtc cgtgggggac tggcccgttc atgttgaaag acggtgtgtc gacgctggac
      721 aagcgcgcac ccgtggtcac cctggtgccg aacccgaact attggaacaa ggcgcgcatg
      781 ccgaaagcca cgttccagtt cgacaacgtg atttccaagg cggaagcgct ggatgaagtg
      841 ataaagggcg gcaaggtcga catcgtgact gaattgtccg ttgccgaagc gaaacaggtc
      901 gtcaagagta agcatgctca tgtggtggaa agcaaggcta agacggtgct ggtaggtgtg
      961 tttaaccaga aaacgacggc ctcgaagtgg aacgacgtta acctgcgtaa ggccgttaac
     1021 attgcggtgg atcgcgctca agtggtgaag aacggcatgg gcggttatgg cacggtgatc
     1081 ccgtccatga ttatgcccgg ccagttcggc tccaacccgg ccctgaaacc ttatgcttac
     1141 aaccccggtg cggcgaagaa ggcgttttcg aaagcgggcg tttccagcgt caccatcgtg
     1201 gctggcgaag ggtacaagga tgtggtggac gcgatcgcca agagcatggc gggagcgggt
     1261 gtgacggtta acgcggtcgc tgaaccgaaa gacaataact gggacatcat gctcgttgag
     1321 cactttgact ggtcgccgga gtacccggta ggcgtggtct accgcgaata ctttggtgag
     1381 gacggcggtt tccgcaaggg accggttgat cccaacctcg cggacctgtt cgccaagata
     1441 tacaaggcca ctaacaaggc caagcaggag gaactggtgc gcggcatcga caagtacgtg
     1501 cacgacaatg cgcaagtgct tttgctgccg tcgccgtcca agctctatgc ggtgagcaac
     1561 aaggtcaatt tcgtgccgta caagaccacc gtgctggagc tggctgagac tacgataaag
     1621 aaatagcccg caacgggtta aatcctgggt tgaatccaga atcgggagcc cgtgccggcc
     1681 gcacgggctc tttttttgtg gagtagaaat ttgattatcg gcaccgcagg gcacatcgac
     1741 catggcaaat ccaccctggt caaggcgttg accggcgtgg acccggtccg cttgcaggaa
     1801 gaaaaagcgc gcggcatcac catagacctc ggtttcgcct accagccgct gagcgacggg
     1861 caggtgctgg gtttcgttga tgtgccgggg cacgagaaat tcatccacaa tatgctcgcc
     1921 ggtgcgaccg ggattgatta cgtgatgttg gtagtggcgg cggacgacgg cccgatgccg
     1981 caaaccgttg agcatctcgc gattctccat ctcctgggct tgtcacgcgg tatcgtcgcg
     2041 ctttccaagg ctgatttggt gagtcaagag aggcttgccg aagtcaaagc ggaaattcag
     2101 tcactgctcg ccccaacccc gctcgcgggg gcgcccattc tcccggtttc ggcggtgagt
     2161 ggagagggca tggctgaatt gcggcaacat ttgctgcgcg cagtttacga ttctggcgtg
     2221 cgcaatcctg gtggccattt ccgccacgcc gttgaccgct gcttcacgct gcaaggggtg
     2281 ggcgtggtgg taaccggcac ggtgttttcc ggcgaggtga agcgtggcga caagcttttc
     2341 gtctcgcctt ctggcattcc ggtgcgtgtg cgcagcatcc atgcacaaaa ccagcaggcg
     2401 acgagtggcc acgtcggaca acgctgcgcg ctcaatctcg cgggcgtcga gaagaatgac
     2461 attaagcgcg gcaactggat actggcggaa gccgtgcacg caccagcgaa gcgctttgac
     2521 gcccacctga atttgctcaa cacggaagtc aagccgctca ggcactggac gtcagtgcat
     2581 gtccacgttg gcgcttgtga cgtgctgggg cgcgtggcgc tgttgcaagc gaataatctt
     2641 cagcctggcg acaacgcgct ggcgcagctc gtgttggatg agccagtcgg cgcgctgcac
     2701 ggcgatcgct ttgtcgtccg cgaccaatct gccatgcgca ccatgggcgg tggcattatc
     2761 attgatccct tcccgccgca acgcggccgg cgcaaggcgc agcgtttgga aatcctttcc
     2821 gcgctggaaa gcgcaaccgc ggcgcaagcg ctgcaaaaac tgctggcatt aagcccattc
     2881 ggtgttgatt ggcaaaaatt tgtgctcacc tggaatctca acgaggccga agcacaaacg
     2941 ctttccaggc aagttgctgt gaccgaggta atttgtgctg agaagcccct ggctttcagc
     3001 agcgaacgtt gggccggatt gaaagcgcaa gcgctgcaag ccttgcaggc atatcaaaag
     3061 cgcttcccca accagaccgg cgcaaccacc gagcaagtct ggcaaggttt tcccgagaag
     3121 ctgcagcagg gattgctgaa tgtggtgatc gggcagctta ttgaagagaa gaagatggtg
     3181 cgcgaaggcc agctgctgcg gatgcccggg ctccaggcca gcctgtcagc cgctgaccag
     3241 aagctgagcg aaaaaataat tccgctgttg caaacggcgg gattgctgcc gcccaaggtg
     3301 cgcgagctgt caacgcagct gcgaacagat gaaaagcagt taaagctgtt gttgcggcgc
     3361 ttttcgcaaa tgggtaagtt gcggcaagtt tccgaagact attattttgc gcctgaagtc
     3421 atcgcaaggc ttgccgccct cacgcagaaa ctcgcgcagc aaagtccgac aggaaccatc
     3481 acggttgggc agtttcgtga gggcacggca gtgtatcgca acctggcgat tccgcttctg
     3541 gaattttttg accgcagcgg attcaccgtg cgcgtggatg acgggcgccg tatccggcgc
     3601 gattggaagg aagtattcgg tgaaaacaaa aaagcttgaa ccgacgcaaa gacgccaaga
     3661 aattccaaat gatttttcct ctgcgcctcc gcgtctccgc ggtgaaaggg ttttagggtt
     3721 tttatggaag ggatgcgttc cccggtgggg cgtccggact tcaaatccgg gaagggccgt
     3781 ctgacggttc ttggtgggtt cgactcccac tctcttccgc catgatttag tgaggcaggc
     3841 aagtgtcaaa caaaacagcg ggcaacaatc tctttgcatt gccttcggtg gaccgggtgc
     3901 tgaattatgc agcgctcgaa ctgctgatag acgaatacgg ccgtgcgtca gtattgaccg
     3961 cggttcgcgc cgtgttaagc gatgcgcgca aggagctgct ggcgggcggt aacggcgcgg
     4021 tttttaatga ggcctggttc ggcatgcact gccgttccca gcttgaggcg caggcgcagc
     4081 cttcgttgcg gccagtgttc aatctcacgg gcacggtatt gcacactaat ctcggccgtg
     4141 cgcttttgcc gcgtgaagcg caggaagcgg cgctggcagt gatggggcag cccgccaatt
     4201 tggaatacga tcttgaatcc ggcgcccgcg gcgagcgtga cgaacacgtg gagggcttgt
     4261 tgtgcaaatt gaccggggcg gaggcggtta ccgtggtgaa caacaatgcc gcagcagtgt
     4321 tcctggtttt gaatacgctg gcgctgaaaa aagaagtgcc ggtttcgcgc ggcgaattga
     4381 ttgaaatcgg cggcgcgttc cgcattcctg acatcatgcg ccgcgccggc tgcaagcttg
     4441 tagaagtcgg caccaccaac cgcacccacc tcaaggattt cgaggaagca ttgaatgacc
     4501 agaccgcgat gctgatgaag gtgcacacca gcaattacgc gattcagggt tttaccgctg
     4561 cggtagcaga aaaggaatta ggcgcgctcg cccacaagca taatctgccg ttcatggtag
     4621 atttgggcag tggcacgctg gtcaatctca aggatttcgg tcttcccgcc gagcccacgc
     4681 cgcagcaagc cttaagtcat ggcgcggaca tcgtgacctt cagcggcgat aaattgctgg
     4741 gcggcccgca agcggggcta atcgtcggca aaaaagaggt gctgaagaaa atcaggaaga
     4801 acccgttgaa gcgtgcgctg cgcgtggaca agattacgct tgcgatgctg gaagcggtgc
     4861 tgcgacttta ccgcgacccg gagcggttga aagaacgctt gcctactttt cggctcttga
     4921 cgcgcccggt tgaagaaatc gaagcgctgg cgaagcggct ttttcccgtg ctggagaaac
     4981 aatttaccgg aaaaggttta gtggcgatta aaccgtgcca cagccagatt ggcagcggct
     5041 cgctgcctgt tgaggtattg cttagcaaat gtattgcaat aactccgccg caaaacgagc
     5101 gccaccccgg aacattgctg gagaaaatcg cggcagcgtt tcgcgcgctg ccggttccgg
     5161 tcatcggcag gattgaagac gacgcgtttt gcctggattt gcgttgcctg gaagacgagc
     5221 agaaattcgt taatcagctc gcaagtctta aaatttagtg caataccgtg ggtggtgttt
     5281 cgcctgtcgc tttggcggtg gccggagcgg ggcaggcgtg atgcagcagc atgcgccagc
     5341 ccctttcggt gagaagatag atattggtgg caataatggg gctgcgcggc gcggtttcac
     5401 ccgtaaccac gatgttttca taaacgctgt gcaccgagag cagcataccg cgcaggtatt
     5461 gctgaccgac gagatgaaag cgtaatctcg ggccattggc gaaaatctgc cgccagcttt
     5521 cacggatctg cccatgtcca accagcctcg gcccggaagg atgcacgcat atcacttcct
     5581 cgtcttccgc ccacaccgcc atcatcgcct ccagatcggc gcgttcaaag gcctcgtaaa
     5641 acgcggtttc tgcgtcctgc ggcgtgggga atatcggcgt tttcatgacg gctcgacttt
     5701 tttaaagtca atcttggcta aaactttttc cggcgtcaat tgcatcatgc agttgaagtg
     5761 cttcaagggg cacacgcgct gaaagcaggg gctgcaggga agttcaagtt tgatcacccg
     5821 cgcttgatcc gagagcggcg gcgtgaagcc ggggctgctt gagccataca gcgcgtaaag
     5881 cggcttgtcc aatgccgcgg cgatatgcat caatccggaa tcgttgctga ccacgagaga
     5941 ggcgcaagcg agcagatcaa tcgcttcatc gagcgtggtt ttgccgcaaa ggttgacaac
     6001 atcgccgccg ctcaagccct ggatttcccc acagatagag gcatcgttgg gcgagccgac
     6061 gagccagatt tgataacctt cgccatgcaa tcgccgggcc agttcggcga aataatttgg
     6121 cggccagcgc ttggcggggc cgtattccgc gcccgggcag aacgcaacta ccggttttct
     6181 cgcgtcgagt ccgagtttct taagaaccgc ttcccgcccg tttgcagata ggcgcaggcc
     6241 gggtgtcgca agcgggcgct tgagcgatgt cccgcgttcc tcgccaagca gcgcaaaacg
     6301 ttcgaccatt aacggaaatt gcagcttgtt cagcgttctg ccgtcattga gcaggccgaa
     6361 gcgcatttcg ccgacaaaac ccgtacgcac cggaatacgc gcgaaaaaag ggatcaatgc
     6421 ggatttccag gagttgggaa gcacgatcgc ctggtcgtag cggcgcgccg ccagtttgtg
     6481 gccgagcctg cggcgatctc gaagattaag ttcgccgtgc cggaaagggt tggtgattac
     6541 ttcgttgact tcgggcatgc gtgaaagcaa gggtgcaacc caaagcgggg cgagtacatc
     6601 tagaacgata ccgggcagcc gttcatgcaa tcttttgaaa agcggttgtg cgagaacggc
     6661 gtcgcctacc caactcggtg cgacaatgag aattctggtc acgataatac cttttcaccg
     6721 ccgaggacgc ggaggaaaag cgctaaagtg ttttttgttt ttcctctgcg acctctgcgc
     6781 tctccgcggt tcaagattta atgcttctcg ggaatcgcgc cgcccttgag agtatagcgc
     6841 gttccgcagt atgggcagag cgcttcgccg gtttgctcaa tcggcaggaa cacccgcgga
     6901 tgggcgttcc acagcaacat tgaaggcatg ggacagtgca gcggcaagtc cttggcggtc
     6961 acttcgatat gccgctcttt attggcagtc ttttcttcct gcatcgccat tcaaacgggg
     7021 gcgagccagt cgcggtgctt gtccgattta ccgttcacgc aatcgaaaaa cattttctgg
     7081 atttttgcgg tgatcgggcc gcgcgagccg gaaccgatgg cgcggccgtc gagctcgcgg
     7141 atcggcgtca cttccgccgc ggtgccggtg aaaaacgcct catctgcaat gtaaatatcg
     7201 tcgcgagtga tgcgcttcgc gctgacctca tacccggctt cgtgcgccag cacgatcacc
     7261 gcatcgcggg tgatgccgat gagcgcggaa gtgagttccg gctcgtacag cctgccgttc
     7321 ttgacgataa acaggttctc gcccgagcct tcggcggcaa agccgtccac gtccaaaagc
     7381 agcgcttcat catagccgtg ctccaccgct tcggtgttgg ccagaatcga gttggcatag
     7441 gtcccggaat acttggcgcg gcacatggtc acgttgacat gatgacgcac gtaggacgag
     7501 gttttcaccc gaatgccttt ttccagccct tccgtgccca gatacgcacc ccacggccag
     7561 gcggcaatcg ccacatgcac cttggcgccc ttgggcgaga cgcctatttt ctccgggccg
     7621 tagaaggcaa tcggccggat gtagcattcc tccagcttgt tcgcgcgcac cacttccttt
     7681 tgcgcctcga ggagggttgg cttgtcgaac ggcatcttca tcaggtaaat gtgcgccgaa
     7741 ttaaacagcc ggtcggtgtg ctccttgaga cggaaaatgg cggtgccgct cacggtcttg
     7801 taggcgcgca cgccttcgaa caccgccaga ccgtagtgga gcgaatgggt caacacatgg
     7861 gtggtagcgt cgtgccaggg caccagcttg ccgtcatacc agatatgacc atcgcggtcg
     7921 gccatggaca tggcgttctc tccacaaatt agacgcggct attctagcgt attttatacc
     7981 gcgtgatgag gagccggacg aaggacagcg atgagcgagc ttgccttgac cggtatcctg
     8041 tcaatggatt aacctagagt ccctgtacat atttcttccc aacaacaact gccatgcctg
     8101 atctggcgca agctaacagc aattccgctg gtattctggg gcggctgact caatcagtaa
     8161 tcagcccgtc tgattctgaa gacgagaggt tgcgcaagac actgctgctg tttgtctgcg
     8221 ggttgatgaa cctggccgca gggttttggc tcatgattta ttggagtatg ggcatgcggc
     8281 ttccgaccac gattccgctg ggctatcagc tcgtctcggc gctgacgctg ataatttacc
     8341 tgaaaaccaa gaatttcgct ttcttccgct ttacccagat cggcctgctg ttgttcgtgc
     8401 ccttcgttat ccaatggagc atcggcaatt tcatcagctc cagcggcatc gtgctgctgg
     8461 ctttgctggc accggtgggg gcgatggttt tccagggggt aagggagtcg attccctggt
     8521 ttgtggccta tgtggtgctc actgcggtct cgggggtttt tgactattat cttgcggcgg
     8581 gggtggtgag cggagtaccg atgagaatca tcccggtgtt ttttgttctg aacttcacca
     8641 tactttcaac catcgtttat ctgctgatcc gattcttcat cctgaaaaaa gatatgtttc
     8701 agaccgagct cggagagaaa aatcggctat tgcaagtgga g
//
LOCUS       DATIOW010000065         3826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_331240, whole genome shotgun sequence.
ACCESSION   DATIOW010000065 DATIOW010000000
VERSION     DATIOW010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331240"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..1484
                     /locus_tag="VLL03_01955"
     CDS             42..1484
                     /locus_tag="VLL03_01955"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HSS46163.1"
                     /translation="MSSHISNIRPKPDQVLVDIADYASKYEIKSKEAYDTARNCLMDT
                     LGCGLEALSYPACAKLMGPIVPGTIVPNGAKVPGTQYQLDPVMAAFNIGCMIRWFDFN
                     DTWLAAEWGHPSDNLGGILATADWISRNNVAQGKKPQVMRDVLTAMIKAHEIQGVLAL
                     ENSFNRVGLDHVLLVKVASTAVVTKMLGGSRDEIIDAVSQAWVDGQSLRTYRHAPNTG
                     SRKSWAAGDATSRAVRLALMTLKGEMGYPSVLTAKTWGFYDVLFKGKEFKFQRSYGSY
                     VMENVLFKISFPAEFHAQTAVECALQLHPQVKNRLDDIKKVAITTHESAIRIIDKKGP
                     LHNPADRDHCIQYMTAIGLIFGNLTAQHYEDKVAKDPRIDALRDKMECVENKQYSKDY
                     LDPNKRSIANAVQIFFKDGSKTEKAEVEYPIGHRRRRTEAIPLLVEKFKTHLARRFPA
                     KRQKAILDLCLDRERLEATPVNEFVDMFVI"
     gene            1610..>3826
                     /gene="acnA"
                     /locus_tag="VLL03_01960"
     CDS             1610..>3826
                     /gene="acnA"
                     /locus_tag="VLL03_01960"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF009520.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HSS46164.1"
                     /translation="MSHNLFNTLREFKLESGKTGKFYSLPALEKTGVGKISRLPVSIR
                     IVLESVLRNVDGKKVTEEHVKQLANWQPNAARVDEIPFVVARILLEDFTGVPLLADLA
                     AMRGVAHKMGKNPKLIEPLVPVDLVVDHSVQVDHYGTRDALDLNMKIEFQRNTERYQF
                     MKWGMQAFDTFKVVPPGIGIVHQVNLEYLARGVHQKNSPQGRVYYPDTLVGTDSHTTM
                     INGIGVVGWGVGGIEAEAGMLGQPVYFLTPDVVGVNLAGRLREGVTATDLVLTITEML
                     RKEKVVGKFVEFFGEGTASLAVRDRATIANMAPEYGATMGFFPVDDATIEYFKGSGRT
                     ADEIDAFCSCFKAQGLFGIPKKGQIDYSKVLELDLASIKPSVAGPKRPQDRIELGLLK
                     NKFIELFSRPVAENGFAKKAEDLSKRYSTRLDTVSSSAEPVSGKDMQNFELLRSGAPA
                     NVVEMVDNHPTPGDAIESSGPSHTVELSHGDVLIAAITSCTNTSNPGVLLAAGLLAKK
                     AVDKGLTVKKHIKTSLAPGSRVVTEYLQKAGLLSYLEQLGFTVAAYGCTTCIGNAGPL
                     TEPIEETIVKNDLVCSAVLSGNRNFEARIHPNLRANFLASPPLVVAFAIAGTVLKNLE
                     SEPLGTGKDGKPVYLKDIWPTSSEVHALMKYANDPATFRRLYSNPTRDHQLWNSVPSA
                     SGQVYDWPKSTYIAEPPFFKDFALDLPAVPADAAQDAVSVRGASIMALFGDSITTDH"
BASE COUNT          880 a         1130 c         1063 g          753 t
ORIGIN      
        1 gcgttcatct gcggtttcaa ttctttaggc agaaaaccac cgtgtcatca catatctcca
       61 acatccgccc caaaccagac caagtcctgg tcgatatcgc agactacgcc agcaaatacg
      121 agatcaaaag caaggaagcc tacgataccg cgcgtaactg cctgatggac acgctgggtt
      181 gcgggctgga agcgctctcc taccctgcct gcgccaagct catgggcccc attgttcccg
      241 gcaccatcgt gcccaacggc gccaaagttc cgggcacgca ataccagctc gacccggtga
      301 tggcggcgtt caacatcggc tgcatgatcc gctggtttga tttcaacgac acctggcttg
      361 ctgccgaatg ggggcatcct tccgacaacc tggggggaat tctcgctacc gccgactgga
      421 tttcgcgcaa caacgtggcg caaggaaaaa aaccgcaagt aatgcgcgat gtgctgaccg
      481 ccatgatcaa ggcgcatgaa atccaagggg tgctggcact ggaaaacagc ttcaatcgtg
      541 tcggcctgga tcatgtgctg ttggttaagg ttgcttcgac ggccgtggtg acgaaaatgc
      601 tgggtggcag ccgcgatgaa atcatcgacg ccgtatcgca agcctgggtg gatggacaat
      661 cgcttcgcac ttaccgccac gcgccgaaca ccggctcgcg caaatcctgg gcagcgggcg
      721 acgccaccag ccgcgcggtg cgcctggcgc tgatgacgct caagggcgaa atgggttatc
      781 cgtcggtgct gaccgccaag acctggggct tctacgacgt gctgttcaag ggcaaggaat
      841 ttaagttcca gcgttcctac ggttcctacg tcatggaaaa cgtgttgttc aagatttcct
      901 ttcccgctga attccacgcg caaacagcag tggaatgtgc attgcagctg catccgcaag
      961 tcaaaaaccg tttggacgat atcaagaaag tggcgattac cacccacgaa tcggccatcc
     1021 gcatcatcga caaaaaaggg ccgctgcaca atcccgccga ccgcgatcac tgcattcaat
     1081 acatgacggc gattggtctc attttcggca atcttaccgc acagcactac gaagacaagg
     1141 tcgccaaaga cccgcgcatt gacgcgttgc gcgacaagat ggaatgcgtg gaaaacaaac
     1201 aatacagcaa ggattatctg gacccgaaca agcgctcgat tgccaacgcg gtgcaaatat
     1261 tcttcaagga cggcagcaag accgagaaag cggaagtaga atacccgatt gggcaccgcc
     1321 gccgccgcac cgaggccatt ccgctactgg ttgagaagtt caagacccac ctggcgcgcc
     1381 gcttccccgc caagcggcag aaagccatcc tcgacctttg tctcgaccga gagcggctgg
     1441 aagccacacc ggtcaacgaa tttgttgata tgttcgtgat ttaaacccat ttaaccgcgg
     1501 aggccgctga ggacgcaaag caaatcgttt tgaattgata atccttagag gttcctctgc
     1561 gtcctttatg ttctttgcgg taaaactttt tgcattttag aggttagcaa tgagccataa
     1621 tcttttcaac actctccggg aattcaagct ggaatccggc aaaaccggaa aattctattc
     1681 cctgcccgct ctggaaaaaa ccggcgttgg caaaatttcg cgcctgccgg tttccattcg
     1741 catcgtgctg gagtcggtgt tacgcaacgt cgacggcaaa aaagtcaccg aagaacatgt
     1801 aaagcagctc gccaactggc agcccaatgc cgcgcgcgtg gacgaaatcc ccttcgtcgt
     1861 ggcgcgcatc ctgctggagg atttcaccgg cgtgccgctg cttgcggatc ttgccgcgat
     1921 gcgcggcgtg gcgcacaaaa tgggcaagaa tcccaagctc atcgaaccgc tggtgccggt
     1981 ggatttggtg gtggaccatt cggtgcaggt ggatcactac ggcaccaggg atgcgcttga
     2041 tctcaacatg aagatcgagt tccagcgcaa taccgagcgc taccagttca tgaaatgggg
     2101 tatgcaggcg ttcgacacgt tcaaagtggt cccgcccggc atcggcatcg tgcaccaggt
     2161 gaatctggaa tacctcgcgc gcggcgtgca tcagaaaaac agcccacaag ggcgtgtgta
     2221 ctaccccgac acgctggtcg gcaccgattc gcacaccacc atgattaacg gtatcggcgt
     2281 ggtcggctgg ggcgtgggcg gcattgaagc cgaggccggc atgctggggc agccggtgta
     2341 tttcctcacg ccggatgtag tgggagtaaa tctggccggg cgcctgcgcg aaggcgtgac
     2401 cgcaaccgac ttggtgctca ccatcaccga gatgctgaga aaggaaaaag tggtgggcaa
     2461 gttcgtcgag ttcttcggcg aaggcacagc ttctcttgcc gtgcgggacc gtgcgacgat
     2521 tgctaacatg gcgcccgagt acggcgcgac gatgggcttc ttcccggtgg acgacgcgac
     2581 gattgaatac ttcaaaggct cgggccgcac cgccgacgaa attgatgctt tctgctcctg
     2641 cttcaaggcc caagggctgt tcggcattcc gaaaaaaggc cagatcgatt acagcaaggt
     2701 gctggaactc gaccttgctt ccatcaaacc ctccgttgcc ggtcccaagc gcccgcaaga
     2761 ccgcatcgag cttgggctgc tcaaaaacaa attcatcgaa cttttttcca gaccggtcgc
     2821 cgagaatggt tttgccaaaa aagccgaaga tctctccaag cgctattcga ccaggcttga
     2881 caccgtcagc agcagcgccg aaccggtatc cggcaaagac atgcagaact tcgaattgct
     2941 caggagcggc gcaccggcca atgtcgtgga aatggtggac aaccacccca cacccggcga
     3001 tgccatcgag tcgtccggcc catcacatac ggttgaactc agccacggcg atgtgctgat
     3061 cgcggcgatt acctcctgca ccaacacttc caatcccggc gtactgctgg cggcgggttt
     3121 gctggcgaag aaagcggtgg acaaaggcct taccgtcaag aaacacatca aaacctcgct
     3181 cgctcccggc tcgcgggtgg tcactgaata tctgcaaaaa gcgggtttgc tctcttacct
     3241 cgagcaatta ggcttcacgg ttgccgctta tggctgcacc acctgtatcg gcaatgccgg
     3301 gccgttgacg gagccgattg aggaaacgat cgtgaaaaac gatttggttt gctccgcagt
     3361 gctctcgggc aaccgcaact tcgaagcgcg cattcatccc aacctgcgcg ctaatttcct
     3421 cgccagtccg ccgctggtgg tggcgtttgc gattgccggc accgtgctca aaaatctgga
     3481 aagcgagccg ttgggaaccg gcaaggacgg caagccggtt tacctcaagg acatctggcc
     3541 cacaagcagc gaagtgcatg cgctcatgaa atacgccaac gacccagcga cctttcggcg
     3601 gctctacagc aacccgacca gggatcatca gctgtggaac agcgtgcctt ccgcttccgg
     3661 gcaggtttac gattggccga aatccaccta catcgccgag ccgccgttct tcaaggattt
     3721 cgcgctggat cttccggccg tgcccgccga tgcggcgcag gacgccgtgt cggtgcgcgg
     3781 cgccagcatc atggcgctgt tcggcgactc gatcaccacc gaccac
//
LOCUS       DATIOW010000066         3414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_637012, whole genome shotgun sequence.
ACCESSION   DATIOW010000066 DATIOW010000000
VERSION     DATIOW010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637012"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            476..856
                     /locus_tag="VLL03_01965"
     CDS             476..856
                     /locus_tag="VLL03_01965"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HSS46165.1"
                     /translation="MERKPERVYSEQEIKERLAKELPHWYYEDGWIRRKYRTGSWKGT
                     LIVVNTVGHLAEAAWHHPDLAVSYAFVIVKLVNHAAKGITDKDFALAKKIEDVIQWQP
                     GKEGGPLEGTPNEDQRFKYIKYDE"
     gene            947..1636
                     /locus_tag="VLL03_01970"
     CDS             947..1636
                     /locus_tag="VLL03_01970"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HSS46166.1"
                     /translation="MNKGTLGFILAGFLACNGTAADLSADQVREQLAQSSEQKPADLS
                     GKDLSNLDLSGLDFKKANLAKANLFGTKLVSANLSGANLSGANLDAAWLMRANFSKAN
                     LTNASLFGPVIYPSLDINPAERPGFSGVNFAGARVIARFSQLNLSGANFAGAHLGVDL
                     KNQPMGQMRNDFSSTDLTGANFKGANLSRADLSSADLTGANLTETNLDGAILKQAKGL
                     DQVKGWDKAVN"
     gene            complement(1664..2968)
                     /locus_tag="VLL03_01975"
     CDS             complement(1664..2968)
                     /locus_tag="VLL03_01975"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012217403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSS46167.1"
                     /translation="MHHIVIVGGGAGGLELATRLGDTLGKKRLAQITLIDKTRTHLWK
                     PLLHEVAAGSMDFDTHSLEYLAQAHWHHFRFRLGNMDGLDRAARQVYVAPAFDEQGNE
                     LIPRCVIGYDTLVICVGSTANDYGTPGAAEHAIALDTAEEAERFRQRLINACIRANAQ
                     AGPLRPEQLHVAIVGGGATGVELAAQLHNTTRQLIVYGLDQIDPEKDVKLNIIEAAER
                     ILPALPERLSTAVEKQLKQLGVQIYAGERVTKVTANAVQTQSGRMIPAELIVWAAGIK
                     APDFLCGLDGLETNSVNQLMVRPTLQTTRDENIFALGDCASCPWPGKNTSVPPRAQAA
                     HQQASFLVKAIKSRLRGHSLPAYRYRDFGSLISLGEYSTVGNLMGWITGGSLFLEGYF
                     ARMMYLSLYKMHLYALHGFIKVFFDTLARLITRRTEPPVKLH"
     gene            3034..>3414
                     /locus_tag="VLL03_01980"
     CDS             3034..>3414
                     /locus_tag="VLL03_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSS46168.1"
                     /translation="MMPVPSLVGMRTFRFEVKHLIAACSLLAVKAVFAADTPTLSVTE
                     IAPGVYVHSGAQEESSPQNLGDIANIGFVVGKRCVAVIDTGGSIVVGRRLRQAIKNTT
                     PVPICYVINTHVHPDHIFGDAAFKE"
BASE COUNT          773 a          955 c          983 g          703 t
ORIGIN      
        1 ctggagcgac gccgagtcag catttgccag cgccaggccc ggtaccgcca gagccagtgc
       61 tgccaatacc aacgacaacc gttttacttg cggtttaatc gtcataacaa cttcctcctt
      121 aagctagaat acatagaagc acagtattat tgactacgac cctaaaaagg acgaactttt
      181 cttgacttac cggctttata gggaaaaggt aacgcgttac attacctgtg gtcactatag
      241 cacattacat gcccgaagca agagccgttt tgctgctttt actcaaccat aattatcgtt
      301 ctaaatacag catgttaaat aaaagcgcgg gggtcgtcgt ggtgtgtcag aaatgaacgt
      361 ggaataccat tgaattaaca ccttgtgtca gaaatgaaag catcagataa ccagtatctc
      421 cccgccataa cagcaagttg acgctgcaaa cgcagtttat aaaagaagtg taatcatgga
      481 aagaaaaccc gagcgggttt actccgaaca ggaaatcaag gagcggctag ccaaggaatt
      541 gccgcactgg tattacgaag acggctggat tcgccgcaaa taccgcactg gaagctggaa
      601 aggcacgctc atagtggtga acacggtggg gcatctggcc gaagcggcat ggcaccatcc
      661 cgatcttgcg gtgtcctatg ctttcgtcat cgtgaagctc gtcaaccacg cggcgaaggg
      721 catcaccgac aaggacttcg cgctggcgaa aaaaattgaa gacgtgattc aatggcagcc
      781 gggcaaggaa ggcggcccgc ttgaaggaac gccgaacgaa gaccagcggt ttaaatacat
      841 caaatacgac gagtagttga agtcgcctaa atccaaacca ggcaagtaac gtgagcagcg
      901 cggaaattca ggggtgagtc agggcaattg tttatgaaga ggcggcgtga ataaaggcac
      961 actgggattt atcttggccg gcttcctcgc ctgcaacgga acggcggcgg atttgagcgc
     1021 ggatcaggtg cgcgagcagc tcgcgcaatc gagcgaacag aaacccgccg atctctccgg
     1081 aaaggacttg tccaacctcg acttgagcgg gctcgatttc aaaaaagcca atctcgccaa
     1141 agccaatctg ttcggcacaa aattggtgag cgccaatctg tccggcgcga acttgtcggg
     1201 cgcgaatctc gatgccgcct ggctgatgcg cgccaatttc tccaaagcga atctgaccaa
     1261 cgccagtctg ttcggtccgg taatttatcc gagtcttgac atcaatcccg ccgagcgccc
     1321 gggtttcagc ggggtcaatt ttgccggcgc acgcgtcatc gcgcgcttca gccagctcaa
     1381 cttgagtgga gccaactttg ccggggcgca cctgggcgtg gacttgaaaa accagccgat
     1441 gggccagatg cgcaatgatt tctccagcac cgatctcacc ggcgcgaatt tcaagggagc
     1501 gaacctgtcc cgcgccgacc tctccagcgc ggatctcacc ggcgcgaatt tgaccgaaac
     1561 caatctcgac ggcgcgattc tgaaacaagc caagggtctg gatcaggtaa agggctggga
     1621 caaggcggtg aactgagaca aggcggtaat aaactgaggg gctttaatgc agcttcaccg
     1681 gcggctcggt gcggcgggta atcagccgtg ccagcgtgtc gaaaaacacc ttgatgaagc
     1741 cgtgcagcgc gtaaagatgc attttgtaga gcgagagata catcatccgc gcgaaatagc
     1801 cttccagaaa gaggctgccg ccggtgatcc agcccatcag attgccgacg gtgctgtatt
     1861 cgcccagcga tatcagtgaa ccgaaatcgc ggtagcggta ggcgggcagg ctgtgtccgc
     1921 gcaatcgaga cttgatggct ttcaccagaa acgaggcttg ctggtgcgcg gcctgcgcgc
     1981 gcggcggcac gctggtgttt ttccccggcc acgggcacga ggcgcaatcg ccgagggcaa
     2041 aaatattttc gtcgcgcgtg gtttgcagcg tgggccgcac catcaattga ttcacggaat
     2101 tggtttccag cccgtcgagc ccgcacagga aatccggcgc tttgatgcct gccgcccata
     2161 cgatgagttc cgccggaatc attcttccgc tttgcgtttg cacggcgttg gccgtgacct
     2221 tggtcactcg ctcgccggcg taaatctgca cgccgagctg cttgagctgc ttttccacag
     2281 cagtcgacaa gcgctcgggc aaggccggca gaattcgctc cgcggcttcg atgatgttga
     2341 gcttgacgtc tttttccgga tcgatctggt cgagcccgta gacgatgagc tgccgcgtgg
     2401 tattgtgcaa ttgcgcggcg agttccacgc cggtggcgcc gccgccgacg atggcaacgt
     2461 gcagttgctc ggggcgcaag ggcccggctt gcgcgttggc gcggatgcag gcgttgatga
     2521 gccgctggcg gaagcgctcc gcttcttctg ctgtgtcgag tgcaatcgca tgctcggccg
     2581 cgcccggcgt gccatagtca ttggcggtgc tgcccacgca aatgaccagc gtgtcgtagc
     2641 caatcacgca ccgtggaatc agctcattgc cttgttcatc gaaagccggc gcaacgtaaa
     2701 cctggcgcgc ggcgcggtct agcccgtcca tgttgccaag acgaaaacgg aaatgatgcc
     2761 agtgcgcctg cgccagatac tcgagcgagt gggtgtcgaa atccatgctg cccgccgcga
     2821 cttcgtgcag cagcggcttc cacaaatgcg tgcgcgtttt gtcgatcagc gtgatctgcg
     2881 ccaggcgctt cttgcccagt gtgtcgccca gccgcgtggc cagctccagc ccgcccgcgc
     2941 cgccgccgac tatcacgatg tggtgcaaga agggaaaatt attcgcgctc atgtaaaata
     3001 tttttctgcg atttgctggt aatagtatca gaaatgatgc ccgttccttc gcttgtcggc
     3061 atgcgcactt tccggttcga agttaaacac ctgattgccg cctgctcgtt gcttgcggtt
     3121 aaagcggtgt ttgccgctga tacgccaacc ctgagcgtga ccgaaatcgc accgggtgtt
     3181 tacgtgcaca gcggcgcgca ggaggaaagc tctccgcaaa acctcggcga catcgccaac
     3241 atcggttttg tcgtgggaaa acgatgcgtg gcggtgatcg acaccggcgg cagcatcgtg
     3301 gtgggccggc gcctgcgcca ggcgattaaa aacaccactc ccgtgccgat ttgctatgtc
     3361 atcaacaccc atgttcatcc cgaccatatt ttcggcgacg ctgccttcaa ggag
//
LOCUS       DATIOW010000067         4367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_840695, whole genome shotgun sequence.
ACCESSION   DATIOW010000067 DATIOW010000000
VERSION     DATIOW010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840695"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VLL03_01985"
     CDS             complement(<1..369)
                     /locus_tag="VLL03_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46169.1"
                     /translation="MKLRLLLWLSFLGVCAWLVLRTPVVADLTAFLPSTATRSQQLLV
                     EQLKSGVAARLMLVAVEGNAPRTLAEVSRKMVQGLRDTGLFNYANNGDAQSIANEREL
                     LLRHRYLLSPAVTPDHFSVPA"
     gene            complement(366..935)
                     /locus_tag="VLL03_01990"
     CDS             complement(366..935)
                     /locus_tag="VLL03_01990"
                     /inference="COORDINATES: protein motif:HMM:NF039988.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-related protein"
                     /protein_id="HSS46170.1"
                     /translation="MYKPLFFFILFLSQAASAGQADWDLPQLMRSMAQVESSRARFTE
                     EKHLAILTAPLTSSGTLAYSRPGFIDKHVLAPYEERMTADGDVLTIEKKGEKRSLALQ
                     NYPIAWAFVESIRATLAGDLALLQRFYRIALEGGRQEWRLTLEPLDAQMADYVQTIKI
                     GGAENRIQSMEILETGGDQSVMAISGSEF"
     gene            complement(935..2164)
                     /locus_tag="VLL03_01995"
     CDS             complement(935..2164)
                     /locus_tag="VLL03_01995"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HSS46171.1"
                     /translation="MSIRENLMRYTWSDEITPGHPALAGHFPGTPVVPGAVLLSRVMH
                     AAEHGFGPDLKIVAVPATKFSAVLRPGEKFDVSLEMAGDSLIRFVITRGEARIASGSL
                     RYLRSQAGKTVSEAWLAQQERGSLTAMRLLIWLTLKLGRNVARIFLYPICAYFIVFSL
                     QARQASRLYLGRVLGRKAGWRDLFRHYHTFASTIHDRVYLLSGQHGYFDVQVQGLDSL
                     ECALERGAGCVLLGSHLGSFEILRAHGTLEKKLPINILMHMENASKINRAAQGLHSEI
                     PERIIPLGKPQTLLQVKECLERGEIVGVLGDRAFQSDKAVSCRFLGDEARFPEGPLLM
                     AALLEVPVVLFFGFYRGGKRYDIYFEAFAKRITLDPSRRSEQLREWVERYVDRLEYYC
                     RQAPYNWFNFYDFWRND"
     gene            complement(2143..3489)
                     /locus_tag="VLL03_02000"
     CDS             complement(2143..3489)
                     /locus_tag="VLL03_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSS46172.1"
                     /translation="MTETYPLLAGHGPESIIACHQGKHISVARFMSEVAQLMTHLPQK
                     RHVLNLCEDRYHFLLGFAAALQAGQVSLLPPSRAEDVLRRLGEDYPDAYCLADHDDVP
                     EGLDVFPVQANEDCTSKTLPVLHIAAAQVAIIVFTSGSTGSPTPHVKNWASLVKVAQA
                     VGERIGLSPGSSILGTIPPQHLYGMETTVLLPLQWGAMMHYGRPLLPADIQSALEQIP
                     SPRWLMTTPLHLRACIAERLKLGGLDGVLSATMPLAPDLAQQVEILWQTRVHDVYGCT
                     EAGIVALRHAASQENWHTLEGVRVYREGEEAWVEGGHIPQPSRLADQISIHNEQEFSL
                     QGRLADLVKIGGKRASLEALNAELNQVAGVMDGIFFIPDEAGAVAERLVAFAVAPGLN
                     AETILAGLRKRIDPVFLPRPLYLVKTLPRNATGKLPRENLRRLAAELSPACPFEKT"
     gene            complement(3501..4073)
                     /locus_tag="VLL03_02005"
     CDS             complement(3501..4073)
                     /locus_tag="VLL03_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46173.1"
                     /translation="MLCFYPAVTHYAVIRQNFALGIGMLVVLILVAAASLPGRSKWLV
                     VAVLPLLLLIPVDPGILLFCPPVIINVLGCGFFGISLREGREPVISRIARLERGELTA
                     ELALYTRRLTWIWTMFFALMALLSVLLAAFAPLAVWSWFSNLVNYLLAGFLFFGEFVY
                     RRMRYRHYHHASPFQVIRSIWDKGSARLKS"
     gene            complement(4120..>4367)
                     /locus_tag="VLL03_02010"
     CDS             complement(4120..>4367)
                     /locus_tag="VLL03_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HSS46174.1"
                     /translation="EREVAQLIVDALNLEITAEQIDPDAPLFGEGLGLDSIDMLEIAL
                     AVSKKYGFQLRSDDASNRRIFASSRSLSAHIGQNRTK"
BASE COUNT          965 a         1301 c         1253 g          848 t
ORIGIN      
        1 ggccgggaca ctgaaatgat cgggggtaac cgcggggctc aacaagtagc ggtggcgaag
       61 cagcagttcg cgctcattgg cgatcgattg cgcatcaccg ttgttggcgt agttgaaaag
      121 gccggtgtcg cgcaatccct gcaccatttt gcggctgact tcggccagcg tgcgcggggc
      181 gttgccttcc accgccacca gcatgagccg ggcggcaaca ccgctcttca attgctccac
      241 cagcaattgc tggctgcgcg tggcggtgga aggcaaaaaa gcggtgagat cggcgaccac
      301 tggggtgcgc agcaccagcc aggcgcacac gccgaggaat gacaaccaca ggagcaaacg
      361 cagcttcaaa attcgcttcc gctgatggcc atcaccgact ggtcgccgcc ggtttcaaga
      421 atttccatgc tttggatgcg attttccgcg ccgccgattt ttatcgtttg cacataatcc
      481 gccatctgcg cgtcaagcgg ctccagagta agccgccact cctgcctgcc gccttcgagc
      541 gcaatgcggt agaagcgttg aagcaacgcc aaatcgccag ccagtgtggc gcggatgctc
      601 tcgacaaaag cccatgcgat cggataattt tgcagcgcca ggctgcgctt ctcgcctttt
      661 ttttctattg tcaggacgtc cccgtccgcc gtcatgcgct cctcgtaggg tgccaggaca
      721 tgtttgtcta tgaatccagg acggctatag gcgagcgttc ccgatgaggt gagcggcgcg
      781 gtcaatattg ccaggtgttt ttcttcggtg aagcgggcgc gcgacgactc cacttgcgcc
      841 atgctccgca tcaattgcgg caaatcccaa tccgcttggc cggcgcttgc cgcttgagac
      901 agaaacaaga taaaaaaaaa cagcggttta tacatcagtc gttgcgccag aaatcgtaaa
      961 aattgaacca attatacggc gcctgccggc agtagtattc gagccgatcg acatagcgct
     1021 ccacccactc ccgcagttgt tccgaacgcc gcgacggatc cagcgtaatc cgcttcgcaa
     1081 aagcctcgaa ataaatgtcg taacgcttgc cgccgcgata aaaaccaaag aacaacacga
     1141 ccggaacttc caggagggcg gccatgagca acggcccttc ggggaaccgc gcctcgtccc
     1201 cgagaaagcg gcaggatact gccttgtcgc tctgaaaggc gcggtcgccg agcacgccga
     1261 cgatttcgcc gcgctcgaga cattctttca cctgcagcaa agtttgcggc ttgcccaggg
     1321 gaatgatgcg ttccggaatt tccgaatgca ggccctgcgc cgcgcggttt atcttgctgg
     1381 cgttttccat gtgcatcaga atattgatcg ggagtttttt ctccaacgtc ccgtgcgcgc
     1441 gcaggatttc gaaactgcca aggtgcgagc ccagcaaaac gcagcccgcg ccgcgttcca
     1501 gcgcgcactc cagtgaatcg agcccctgca cttgcacgtc gaaatagccg tgttgtcccg
     1561 acagcaaata cacccggtca tgtatggtgg aagcaaaagt gtggtagtgc ctgaacaggt
     1621 cgcgccagcc ggcctttcgt ccgagcacgc ggcccaggta caaacgcgaa gcctgccttg
     1681 cctgcaggga aaaaacaata aagtacgcgc atatggggta caggaatatc cgcgcgacgt
     1741 tacggccaag cttcagcgtc agccaaatca gcagccgcat ggcagtgagg ctaccgcgct
     1801 cctgctgcgc aagccaggcc tcactcactg ttttccccgc ctgagaacgc aagtaacgca
     1861 agctgccgct tgcgattcgg gcctcgccgc gtgtgatgac aaaccgaatc aggctgtcac
     1921 ccgccatttc caggcttaca tcgaattttt cgccggggcg gaggaccgcg ctgaacttgg
     1981 ttgcgggcac cgccacaatt tttaggtccg gcccaaagcc gtgctctgcc gcgtgcatga
     2041 cccggcttaa cagcaccgcg cccggaacaa ccggagtgcc cggaaaatgt cccgccagcg
     2101 ccggatggcc gggggtaatc tcgtcgctcc aggtataacg catcaagttt tctcgaatgg
     2161 acatgcaggc gacaactcgg cggcgagcct gcgcaggttt tcacgcggca gcttgccggt
     2221 ggcgttgcgc ggcagtgttt tcaccaggta cagcggacgc ggcaaaaaaa ccgggtcaat
     2281 gcgcttgcgc agtccggcga gaatcgtctc ggcgtttaat cccggcgcca cggcaaaagc
     2341 aactaggcgt tcggctacgg caccggcttc atccgggatg aaaaatattc cgtccatcac
     2401 acccgctacc tggttaagct cggcattgag cgcctcgagc gaagcacgct ttcctccaat
     2461 tttcaccaaa tcggcgaggc ggccttggag gctgaattcc tgctcgttgt ggatgctgat
     2521 ttggtcggcc agccgtgacg gttgcgggat atgcccgcct tcgacccagg cttcctcacc
     2581 ctcccggtaa acccgcacac cttccaacgt gtgccagttt tcttgcgaag cagcatgacg
     2641 cagcgccacg atgccggctt cggtgcaacc gtatacgtcg tgcacccggg tttgccacag
     2701 gatttccact tgttgggcca ggtcgggcgc taacggcatg gtcgccgaaa gcacgccgtc
     2761 aaggccgccg agtttcaacc tttcggcgat gcacgcgcgc aaatgcaagg gggtagtcat
     2821 gagccagcgc ggggacggga tttgttccaa cgccgactgg atgtccgccg gcagcaacgg
     2881 ccgcccgtag tgcatcatcg cgccccattg cagcggcaac aggacggtgg tttccatgcc
     2941 gtacaaatgt tgcggcggaa tggttcccaa aatactcgat ccgggcgaca aacctatccg
     3001 ttcgcccacg gcctgcgcta ctttgacgag cgaagcccag ttttttacat gcggcgtggg
     3061 gctgccggtg ctgcccgaag tgaaaacaat tatggcgacc tgcgcggcgg ctatatgcag
     3121 gacaggcaaa gtttttgaag tgcaatcttc attcgcctgc accgggaaaa catcaagccc
     3181 ttccggtacg tcgtcgtgat ctgcaaggca atacgcgtca ggatagtcct cgccgagcct
     3241 acgcaatacg tcttccgcgc ggctcggcgg cagcaggctc acctggcccg cttgcagggc
     3301 ggcggcaaag cccagcagga aatgataacg gtcttcgcac aggttcaata catggcgctt
     3361 ttgcggtaga tgcgtcataa gttgcgcaac ctcgctcatg aaacgcgcga cactgatgtg
     3421 tttgccttgg tggcaggcaa tgatactttc cggtccgtgc ccggcaagca agggataggt
     3481 ttcggtcatt gggaaattgg ctaggattta aggcgcgctg agcctttgtc ccagatgctg
     3541 cggataactt gaaaaggtga agcgtgatga taatgccggt agcgcatgcg ccggtaaaca
     3601 aattccccga agaacagaaa ccccgccagc agataattaa ccaggttgct aaaccatgac
     3661 cagaccgcta gcggagcaaa agccgcgagc aggactgaaa gcagggccat cagggcgaaa
     3721 aacatggtcc aaatccaggt aagccgccgt gtatacaggg caagctcggc agtcagttcc
     3781 ccgcgctcca gccgggcgat ccggctgatg acgggttcac ggccctcgcg cagacttata
     3841 ccaaaaaacc cgcatccaag cacattaatg ataaccggcg ggcagaagag caggattccg
     3901 gggtctaccg ggatcagcag tagcagaggc aaaaccgcca ccaccagcca tttgcttctc
     3961 cccggcagcg atgccgcggc aaccaggatc agtaccacca gcatgccgat gccgagggca
     4021 aagttttgac ggattaccgc gtaatgcgtg acggccggat agaagcagag catgatgacc
     4081 catacccata tgcgtagcgg cagcgcggaa ccagtcggct tatttggtac ggttttgtcc
     4141 aatgtgggcc gaaagagagc gcgatgaggc aaaaatacgc cggttgctgg cgtcgtccga
     4201 gcgcagctgg aaaccgtatt ttttggagac cgccagcgcg atctccagca tgtctatgga
     4261 atcaaggcca agaccctcgc caaacaaggg ggcatccgga tcaatttgtt ccgcagtgat
     4321 ttccagattc agcgcatcga cgatgagctg cgccacttct ctttcca
//
LOCUS       DATIOW010000068         1823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1246571, whole genome shotgun sequence.
ACCESSION   DATIOW010000068 DATIOW010000000
VERSION     DATIOW010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246571"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VLL03_02015"
     CDS             <1..515
                     /locus_tag="VLL03_02015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HSS46175.1"
                     /translation="LELGTGSGNIALALARYRPLARITAVDCSEEALAVARQNAEQLL
                     GADNRAVMFKNSDWFDALGGDTCDLIISNPPYVASGDAHLQQGDVRFEPRQALDGGAG
                     GLDCIRRIIQEAPSRLRKGGWLLFEHGYDQADACQELLSRSGFTRLFCANDLAGIPRV
                     SGGRIDDGML"
     gene            548..853
                     /gene="grxD"
                     /locus_tag="VLL03_02020"
     CDS             548..853
                     /gene="grxD"
                     /locus_tag="VLL03_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HSS46176.1"
                     /translation="MSAQELIKKQVTEHPVVLYMKGTPDFPQCGFSANVVNILKACGV
                     ENMFTVDVLSNPEIRQGVKEYANWPTIPQLYVNGEFVGGSDILTEMYQSGELQKLFK"
     gene            929..>1823
                     /locus_tag="VLL03_02025"
     CDS             929..>1823
                     /locus_tag="VLL03_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HSS46177.1"
                     /translation="MKIKAAVLNQMGVKPPYAASRPLSVEEVVLDPPGPGEVLVKIAA
                     AGLCHSDLSVINGDRPRPTPMVLGHEAAGIVEELGAGVDDLKKGEHVVMVFMPSCGHC
                     LPCAEGRPALCEPGAAANVAGSLLSSARRLKRGATPLYHHMGVSAFAQYATVSRRSLV
                     KIDSDLPLAEAALFGCAVLTGVGAVLNTARVTPGSTVAVIGLGGVGLASVLGAVLAGA
                     RRIVAIDLAEDKLALAKSLGATETYNVASPDCTKQVRAATEGGVECAFELAGSVKAME
                     LAYKITRRGGTTVTAGLPPPAD"
BASE COUNT          353 a          506 c          588 g          376 t
ORIGIN      
        1 tgctggagct gggcacgggc agcggcaaca tcgcgcttgc tcttgcccgg taccgcccgc
       61 tggctcgcat taccgcggtg gattgctcgg aggaggcgct cgctgtagcg cggcaaaatg
      121 ccgaacaact gctcggcgcc gacaaccggg ctgtgatgtt taaaaacagc gactggttcg
      181 acgcactagg cggagatacc tgcgatctca ttatttccaa tcctccttat gtcgcttctg
      241 gtgacgcgca tttgcagcag ggcgatgtgc gctttgagcc caggcaggcg ctcgacggcg
      301 gagccggcgg gctcgactgc attcgtcgga ttattcagga ggcgccgtcg cgcctgcgca
      361 aaggcggctg gctgcttttt gaacatggct acgatcaggc ggacgcctgc caggaattgc
      421 tctcgcgaag cggatttacc cgcctctttt gcgctaacga cctggcggga attccgcgcg
      481 tttcaggcgg ccgcattgac gatggaatgt tataatactt gcagatgttt cattttcagg
      541 agtggttatg agcgcgcagg aactcatcaa gaagcaggtc accgagcacc cggtagtgct
      601 gtacatgaaa ggcacgcctg attttccgca gtgcggattc agcgccaatg tggtgaatat
      661 cctgaaggca tgcggagtgg agaatatgtt cacggtggat gtgctttcca atccggaaat
      721 ccgccaggga gtcaaggaat acgccaattg gccgaccatc cctcagcttt acgtcaacgg
      781 cgaattcgtc ggcggttcgg acatcctgac cgagatgtat caaagcggcg agctgcagaa
      841 gttgtttaaa taatttttat caaacgcaga aaagccggct gcgccggctt ttttgtctgc
      901 atcaccggcg attcagggga gattgccaat gaagattaaa gcagcggtgc tgaatcagat
      961 gggcgtcaag ccgccctatg cggcatcaag accgctgtcg gtcgaggaag tcgtgcttga
     1021 tccgcccggg cccggagaag tgctggtgaa aatcgccgcc gcgggtttgt gccattccga
     1081 tttgtcggtc atcaatggcg accggccgcg tcccaccccc atggtgctgg gccacgaagc
     1141 agcaggaatt gtcgaagagt tgggcgcggg agtagatgac ctcaaaaagg gcgaacatgt
     1201 ggtgatggtg ttcatgccga gttgcggtca ttgcctgcct tgcgcagaag gtcggcctgc
     1261 gctgtgcgag cccggcgccg cggccaatgt cgcaggctcg ctgctctcca gcgcgagacg
     1321 gctcaagcgc ggcgcaacac cgctctatca ccacatgggc gtttcggctt ttgcccaata
     1381 tgcgacagtg tcgcgccgct cgctggtgaa aattgattcc gatttgccgc tggccgaagc
     1441 ggcgctcttc ggctgcgcag tgctcacggg cgtcggagcg gtgcttaata cggcgcgcgt
     1501 cacgccgggc tcgaccgtgg cggtgattgg cttgggcgga gtggggctgg cttcagtcct
     1561 cggcgcggtt ctcgccggtg cgcgccggat tgtcgcgatt gaccttgccg aagacaagct
     1621 cgcgctcgca aaaagcctgg gcgcgactga aacctacaat gttgcatcac cggactgcac
     1681 gaagcaagtg cgcgcggcga ctgagggcgg cgtggaatgc gcatttgaac tggcgggctc
     1741 ggtgaaagcg atggaattgg catacaagat tacgcgccgc ggcggcacca cggtcacggc
     1801 cggcttgccg cctcccgcag ata
//
LOCUS       DATIOW010000069        13138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_841376, whole genome shotgun sequence.
ACCESSION   DATIOW010000069 DATIOW010000000
VERSION     DATIOW010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13138
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841376"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VLL03_02030"
     CDS             <1..842
                     /locus_tag="VLL03_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HSS46178.1"
                     /translation="RLLPECSRNRLQIWIREKRVWQNGAPAIAKRRVWGGEKIRIAAV
                     PAAETLAHLPEDIPLDIIHEDDSLLVVNKPPGLVVHPGSGNWRGTLLNALLKHSPGLA
                     EIPRAGIVHRLDKDTSGLLVVAKTLEAQTNLVRQLQARSVKREYLALVQGKVVRGGQV
                     EAPIGRHPFARTRMAVVARGKPALTYYEVMERFAHCTLLCCRLETGRTHQIRVHMQSI
                     GHPLVGDPVYSAKKKAPAFARQALHAYHLALLHPRNGKTMGWKAGLPADMRQLLESLR
                     GQN"
     gene            939..1697
                     /gene="pgeF"
                     /locus_tag="VLL03_02035"
     CDS             939..1697
                     /gene="pgeF"
                     /locus_tag="VLL03_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HSS46179.1"
                     /translation="MRAPAEWIIPQWPAPAHVRALITTRGKNGASSGPYAQFNLGGYV
                     GDEPSAVLHNRKLLRAYLPSEPKWLKQVHGTRVLDADVSAAGEESDAAVARQSGTVCV
                     ILSADCLPLLLCDEQGTVIAAAHCGWRGLAAGVIEQTIRAMQAPPASLLAYLGPAIGP
                     HAYEVSADVHDAFLRGDAGAEQAFVPRASGKWLANLYSLARQRLNRLGVKKISGGDYC
                     THTDKARFYSHRRDGRTGRMASLIWLDTKTDEFC"
     gene            1737..2396
                     /locus_tag="VLL03_02040"
     CDS             1737..2396
                     /locus_tag="VLL03_02040"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HSS46180.1"
                     /translation="MSILAWIVCASLAGGVLSVLCAAGVALNTRAAWLPMLVSYAIGA
                     LLGAVFLEILPHAFELSRNVEHMTATILFGILLFFVLEKLVLWRHCHQNECEAHGGDD
                     PRHDHGRSGMMIMIGNTFHNFVDGVLIAAAFLADVRLGIVTSLAIIAHEIPQQVGDFL
                     ILLHSGYTRKQALVFNLLSGTATLAGGLLAYFTLHTCSRWCRRSWAWQWQACSMSRSR
                     I"
     gene            2369..2512
                     /locus_tag="VLL03_02045"
     CDS             2369..2512
                     /locus_tag="VLL03_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46181.1"
                     /translation="MLYVAVADLIPGLHRRPELHATLQQVLLITLGIGSIWLTRSLSG
                     HLA"
     gene            2794..4557
                     /gene="phaC"
                     /locus_tag="VLL03_02050"
     CDS             2794..4557
                     /gene="phaC"
                     /locus_tag="VLL03_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HSS46182.1"
                     /translation="MSSQPESQSLPPDNEASRNLLQDLLKNLSSQPNLDSAGEIFQLL
                     TAGAAQDTQRLMDIHNHFYQKQVDLWMKMLERKPGETCPPVVAPEKGDRRFQSAEWSA
                     LPLFDYLKQYYLISSQWLLEIVEAARLDPEAKKKLRFFTRQYIDALSPANFPATNPEV
                     LKLAAESNGATISRGLKNLIEDLEKGHISMTDESAFEVGRNLATTSGAVVFENELIQL
                     IQYKPLTETVGELPLLIIPPCINKYYVLDLQPENSFVRHCLEQGNTVFIVSWRNIPHE
                     LGHLTWDDYLKLGVIQAIDAALAIAGVKKLNTLGFCVGGTLLACALAALRAKRRNAVN
                     SLTLLATMLDFTDTGEISAYVDEAYVQKREAGIGRGGIIPGRELALAFASLRANELVW
                     FYVVNNYLKGKTPDAFDLLYWNCDGTNLPGPMYVYYVRNMYLENSLKIPGKLTMCGVP
                     VDLTKINLPTYVLAAREDHIVPWKSAYASASLLKSRIEFVLTASGHIAGVVNPPAKSK
                     RSFYSGGALDTDAEAWLAATQSHPGSWWPHWSAWLAQKRGKEIPARAKPGNKSHREIE
                     AAPGRYVSERCAGNDFKHEAG"
     gene            4602..5348
                     /locus_tag="VLL03_02055"
     CDS             4602..5348
                     /locus_tag="VLL03_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HSS46183.1"
                     /translation="MAKQRIVLVTGGMGGLGESICTKMADQGYRVVATYSSNNPKAGQ
                     WAAEMKSRDYEFYTAQVDVSDFDSCARMVAKVEKDVGPIDVLVNNAGITRDMTFKKMA
                     KADWDAVIKTNLDSVFNMTKQVLDGMVERGWGRVINVSSVNGQKGAFGQTNYAAAKAG
                     MHGFTKALAQEVAKKGVTINTISPGYIGTKMVMAIPKEVLDSKILPQIPVGRLGKPEE
                     VAGLIIYLASDEAAFVTGANISINGGQHMY"
     gene            complement(5438..5980)
                     /locus_tag="VLL03_02060"
     CDS             complement(5438..5980)
                     /locus_tag="VLL03_02060"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HSS46184.1"
                     /translation="MFNEQFPELNKSSLENALRFARITMESAERLVKLQLETAKQAIE
                     ENAKNTKALSEVKDLQEVVALRTKLAEAGVEKALSYSRNVYEVASQAQAELTKLFEES
                     LSAYTKDLVSAIEKTSKSAPAGSDLAVAALKSTVAATQAAVDSMTKAARQVAELADTG
                     VKAAHSATSSAVKGGSKKNH"
     gene            6216..6806
                     /gene="phaR"
                     /locus_tag="VLL03_02065"
     CDS             6216..6806
                     /gene="phaR"
                     /locus_tag="VLL03_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HSS46185.1"
                     /translation="MNDTARVIKKYPNRRLYDTTSSSYITLADVKQLVLDHVDFRVVD
                     AKTNEDLTRSIMLQIILEEESGGMPMFSSDMLSKIIRFYGHAMQGMMGVYLEKNIQTF
                     IEMQQRLQDQSHAMYGDNPMLNAETWAQFLKFQGPAIQGLMTSYLEQSAKMFLDMQSQ
                     LQKQTRNIFGNFTFPNFGVTPQEPPAAGPNKEEDKK"
     gene            6837..8156
                     /gene="rimO"
                     /locus_tag="VLL03_02070"
     CDS             6837..8156
                     /gene="rimO"
                     /locus_tag="VLL03_02070"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HSS46186.1"
                     /translation="MTAVPKVGFVSLGCPKALVDSERILTHLRAEGYLIAPSYEEADL
                     VVVNTCGFIDSAVEESLNAIGEALDENGRVIVTGCLGAKADVVRRAHPQVLAVTGPHS
                     AQEVMDAVHAHLPQPHDPYTSLIPPQGVKLTPAHYAYIKVAEGCNHRCTFCIIPSMRG
                     NLMSRPINKVMQEAENLVKAGVKELLVISQDTSAYGVDIKYRTGFWRGRPLKTRMAEL
                     ARSLSELGVWVRLHYVYPYPHVDEVIPLMAEEKLLPYLDVPFQHASPRILKAMKRPAG
                     AQCNLARIRAWREVCPDITIRSTFIVGFPGETEEDFEQLLGFLEEAQLDRVGCFTYSP
                     VDGAAANALPGHVPEEVKEERKARFMTLQEKISTERLKRKIGRTMTVLVDEITGDSAV
                     ARSSADAPEIDGRVYLKNSRGLVAGELVKVKITASDAHDFWAAPLKN"
     gene            complement(8158..8703)
                     /locus_tag="VLL03_02075"
     CDS             complement(8158..8703)
                     /locus_tag="VLL03_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HSS46187.1"
                     /translation="MKSKNLLLLNLGQICGLLLLSPAAQAQNSMCQLQPNAYCQNADL
                     RGANLQFAQLYESNFDGANLEKTNLQNAFLYNSSLQGANLQGVNLTSASMSHVNLKRA
                     NLKEGNLYRTNLLGADLEGADLQGANLVKANLKGANLKGANLAAAILKGANLEGADLQ
                     GANLQGANFVGALTDNCKGCP"
     gene            complement(8700..10655)
                     /locus_tag="VLL03_02080"
     CDS             complement(8700..10655)
                     /locus_tag="VLL03_02080"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HSS46188.1"
                     /translation="MLNLDSIFSDRGPLAQAVRDFRVRQGQLEMALAVADAIAGNSVL
                     VAEAGTGTGKTFAYLAPALLSGGKVIISTGTKTLQDQLFNRDIPTVRAALKAPVTIAL
                     LKGRANYICHYRLNQALNEGRFGSREDAGYLPPIAQYTRTSSSGDKSGLAEVPENAGV
                     WPHVTSTRENCLGQECPNFQECFVMKARKEAQQADVVVVNHHLFFADVMLRDEGVAEL
                     LPACNTVIFDEAHQLPETASLFFGESISTAQILELARDTRTEGIANARDFTVLPEAAS
                     ALDKAARDLRLTVNEDNARLSQQSLQDISGFKAALDGLCEKLDALTGLLESQAERGEG
                     LRHCWQRGLELAAKIARWRDAEDTTQVRWGEVFAHSMQLNSTPLSIAEIFRKQVEGNA
                     RALIFTSATLSVNRDFSHYQNEMGLHDAATASWDSPFEYEQQALLYLPQALPDPNSGE
                     YTQAVIKAALPLIKASHGRAFLLFTSLRAMQEARTLLKEYFHEAKLDYPLLVQGEGSR
                     SELLERFRRLGNAVLVGSQSFWEGVDVRGPALSLVIIDKLPFAPPDDPVLAARIEQIN
                     RQGRNAFMEYQLPRAVITLKQGAGRLIRDETDRGVLMICDPRLLSKSYGRRIWRSLPP
                     MRRTREETEAVDFLEQCHPVMQTESAK"
     gene            10910..11254
                     /locus_tag="VLL03_02085"
     CDS             10910..11254
                     /locus_tag="VLL03_02085"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HSS46189.1"
                     /translation="MLEHTANRVVSEMPIRPSILNPFGVVHAGAMLWFADVSATLLVI
                     GTATASEGMKGFPLVINLNANFIGNQADGSFKAVASFVKRGRTVSIVRTLVYGEGDKL
                     IADVSTNHVLSK"
     gene            11377..11607
                     /locus_tag="VLL03_02090"
     CDS             11377..11607
                     /locus_tag="VLL03_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HSS46190.1"
                     /translation="MDTQKRATVYFESELHRALRLKAAALDRSISDMVNDAVKAALAE
                     DAEDLTAFSERKKDRNLSFDTFVQGLKRRGRI"
     gene            11594..11857
                     /locus_tag="VLL03_02095"
     CDS             11594..11857
                     /locus_tag="VLL03_02095"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HSS46191.1"
                     /translation="MAEYRLLIKASAAKEIEAVGTKADRQRIVERIRALAQDPRGQGL
                     EKLAGSANRYRARQGRYRIIYLIDDGRREVTIFKIGHRKDVYR"
     gene            11931..12527
                     /locus_tag="VLL03_02100"
     CDS             11931..12527
                     /locus_tag="VLL03_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46192.1"
                     /translation="MEFDSLIPLWIKLAYTLFVAITVAVYATKYSRDNFLWFSDIALL
                     ATVAALWFESPFLASMMAVGVLLPEALWNISFFGQLLTGKRVSGLTDYMFDARKPLYL
                     RALSLFHVFLPALLIWMIARLGYEPGAWIAQTALAWFVLPLCYWLTDPAENVNWVFGP
                     GNQPQKRMAPLAYLGLLMVGFPVLIYLPTHLLLRSLFG"
     gene            12607..13101
                     /locus_tag="VLL03_02105"
     CDS             12607..13101
                     /locus_tag="VLL03_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46193.1"
                     /translation="MAARKKAKAKVKRKKTPARRPATLKKTAQVRKKAAGKKPTVRHV
                     APKKKPAARPSPKARPARTPPRPTGAEASAPLAGEERVGTVTHYYSHLSVAVVQMEAG
                     SLRVGDTIHVWGHTSDFRQRVESMQIEHQPVFEVSAGQEFGLGVTDHARERDVVYKVT
                     APLR"
BASE COUNT         2881 a         3827 c         3707 g         2723 t
ORIGIN      
        1 agcgtttgtt gccggaatgc tccaggaacc ggttgcaaat ctggatacgc gaaaaacggg
       61 tgtggcaaaa cggcgcgccc gccatcgcca agcgcagggt gtggggcggg gaaaaaatcc
      121 gtatcgccgc cgtgcccgcc gctgaaacgc tggcgcattt gccggaggat atcccgctcg
      181 acattattca tgaagatgat tcactgctgg tcgtcaataa accgccgggg ctggtggtgc
      241 atccgggcag cggcaactgg cggggaacgc tgctcaatgc gctgctcaag cactcacccg
      301 ggcttgccga aatcccgcgc gccgggatcg tgcaccggtt ggacaaggac accagcggtc
      361 ttctggttgt cgcgaaaacc ctcgaggcgc aaaccaatct ggtgcggcaa ttgcaggcgc
      421 gtagcgtgaa gcgcgaatat ctggcgctgg tgcagggcaa ggtggtgcgc ggcggccaag
      481 tcgaagcgcc gattggccgc catccctttg cccgcaccag aatggcggtg gtcgcgcgcg
      541 gcaaacccgc attgacttat tacgaggtga tggaacgctt tgctcattgc accttgctgt
      601 gctgccggtt ggaaaccggg cgtacgcacc agatccgcgt gcacatgcaa tcgatcggcc
      661 acccgctggt cggcgacccg gtttattccg cgaaaaagaa ggcgcccgca ttcgcccgcc
      721 aggcgctgca tgcgtaccat ctcgccttgc tccaccctcg aaacggaaaa accatgggct
      781 ggaaagccgg gttgccggcc gatatgcgac aattgcttga atctctgcgc ggccaaaact
      841 gagaaaacag tgctttgcgc gcaggcacag tcccccaata aactgcggca aaaaacttgc
      901 cggtctctcc agtccccgtg actcaaggag cgaacagcat gcgcgcaccg gcagagtgga
      961 tcatcccgca atggcccgct cccgctcatg tgcgcgcgct catcaccacc cgcggaaaaa
     1021 acggcgcgag ttccggaccg tatgcccaat tcaatctcgg cgggtatgtg ggcgatgagc
     1081 cttccgcggt tttgcataac cgtaaattgc tgcgcgctta tttgccgtcg gaacccaaat
     1141 ggctgaagca ggtgcatggc accagggtgc tggacgccga tgttagtgct gctggcgaag
     1201 aaagcgacgc cgcggtggcc cggcaatccg gcacggtctg cgtcatattg agcgccgact
     1261 gcctgccgct tttgctctgc gatgagcagg gcacggtgat cgcggcggcg cactgcggct
     1321 ggcgcgggct ggcggctggc gtgatagagc aaacgatcag agccatgcag gcaccacccg
     1381 cttctctgct cgcttatctg gggccggcca tcggccctca tgcctatgaa gtcagcgccg
     1441 acgtgcacga tgcgttcctg cgcggcgacg ccggggcgga gcaggctttc gtcccccgcg
     1501 cttccggtaa atggctggcg aatctttatt ctctcgcgcg gcaacgctta aaccgtctcg
     1561 gcgtgaaaaa aatcagcggc ggcgattatt gcacccatac cgacaaagcc cgcttctact
     1621 cgcatcgccg cgacggccgc accggacgca tggcctcgct catctggctg gacacaaaaa
     1681 cggatgaatt ttgctagtag caggtataat caaaaattaa ttttctcttt ccaccgatgt
     1741 ccatcctggc ctggatagtc tgcgcaagtc tcgccggcgg cgtgttgagc gtgctgtgtg
     1801 cagcgggagt cgccctgaac acccgtgccg cgtggctgcc gatgctggtg agctacgcta
     1861 tcggcgcgct gctcggtgct gtattcctgg aaatcctgcc gcatgccttc gagctctccc
     1921 gcaatgtgga gcacatgacc gctaccattc tgttcggcat cctgctgttc ttcgtgctgg
     1981 aaaagctggt gctttggcgc cattgccacc agaacgaatg tgaagcacac ggcggcgacg
     2041 atccccggca cgatcacggc cgcagcggca tgatgatcat gatcggcaac accttccaca
     2101 atttcgtcga cggcgttctg attgcggccg cttttctcgc cgacgtgcgg ctcggcatcg
     2161 tcacctcgct cgccattatc gcgcacgaaa tcccgcagca agtcggcgat tttctgattc
     2221 tgctgcactc ggggtacacg cggaaacaag cgctggtgtt taatctgctc tccggcaccg
     2281 cgacactggc gggcggcctg ctggcttatt tcactttgca tacatgcagc agatggtgcc
     2341 gccgctcctg ggcttggcag tggcaagcat gctctatgtc gcggtcgcgg atttgattcc
     2401 ggggctgcac cggcggccgg aactgcatgc cacgctacag caagtgctgc tcatcacgct
     2461 gggcatcggc tccatctggc ttacgcgcag cctctccggc catctggcgt agcttatttc
     2521 gcttgcgctg cggcgtgccg atcagccaca agcgcaagcg gagcgaggcc gtaaaaaaca
     2581 aaagtaagca ggcccgccac cacgctggtt tcggtgaatg ccatcgcacc caccacgtaa
     2641 agccaggcaa tcgccgtaca tgagcagaac tctccgctta atccgcgcca ctatcccacg
     2701 ccaatgcagt cgctttcaat caccccgctt cacttgacag ggcgccgcct cgcagcgcaa
     2761 agtaatattt aaaaacatct tcctattctc cccatgagct cgcaaccgga atcgcagtct
     2821 ctaccgcccg acaacgaggc gagccggaat ctcctgcaag acttacttaa aaatctttcg
     2881 agccagccca atctcgactc ggcgggcgaa atttttcagc tgctcaccgc cggcgcggcg
     2941 caggacacgc agcgcctgat ggacatccac aaccactttt accagaagca ggttgatttg
     3001 tggatgaaga tgctggaacg caaaccgggc gaaacctgcc cgccagtggt ggcgcccgaa
     3061 aaaggcgacc gccgcttcca atctgcagaa tggagcgccc tgccgctttt cgattatctc
     3121 aagcagtact acctgatctc ttcgcagtgg ctgttggaaa tagtggaagc cgcgcggctc
     3181 gatccagagg ccaagaaaaa actgcgcttt ttcaccaggc agtacattga cgccctgtcg
     3241 cctgccaatt ttccggcgac aaatccggaa gtgctgaaac tcgcggcgga gagcaacggc
     3301 gcgactatca gccggggctt gaaaaacctc atcgaggatc tggaaaaagg ccatatctcg
     3361 atgaccgacg agtcggcctt cgaagtcggc aggaatctgg ccacgacgtc cggggcagtg
     3421 gtgtttgaaa acgaactgat tcagcttatc cagtataagc cgctcaccga aaccgtgggg
     3481 gagttgccgc ttctcatcat tccgccctgc atcaacaagt attatgtcct tgacctacag
     3541 ccggaaaatt ctttcgtgcg tcattgcctg gagcaaggca acacggtgtt catcgtttcc
     3601 tggcgcaata ttccgcatga acttgggcat ctgacttggg acgattacct caaactcggt
     3661 gtaattcagg caattgacgc cgcgctcgcc attgcaggag tcaaaaaact caacacgctg
     3721 ggcttctgtg tcggcggtac actgctcgcc tgcgcgctgg cggcgctgcg cgccaaacga
     3781 cgcaatgcag tgaacagcct caccctgctc gccaccatgc tggatttcac cgacaccgga
     3841 gaaatcagcg cttatgtgga cgaagcctat gtgcagaaac gcgaagccgg cattggtcgc
     3901 ggcggaatta ttccggggcg tgaactggcg ctggctttcg ccagtctgcg cgccaacgag
     3961 ctggtgtggt tctatgtggt aaacaattac ctcaagggca aaacccccga tgcctttgac
     4021 cttctttact ggaattgcga cggcaccaac ctgccgggcc cgatgtatgt ttactacgtg
     4081 cggaacatgt atctggaaaa cagtctgaaa atacccggca aactcaccat gtgcggcgtg
     4141 ccggtagatc taaccaaaat caatctgccg acttatgtgc tggcggcgcg tgaagatcac
     4201 atcgtgccgt ggaaatccgc ctacgccagc gcgtcgctgc tcaaaagccg aatcgagttt
     4261 gtgctcaccg ccagcgggca tatcgccggc gtagtcaatc ccccggcgaa aagcaagcgc
     4321 agcttttata gcggcggggc tctcgatacg gacgcggaag cgtggcttgc cgctacgcaa
     4381 tcccatccgg gaagctggtg gccgcactgg agcgcttggc ttgcgcaaaa gcgcggcaag
     4441 gaaattccgg cgcgcgccaa gccgggcaac aaaagccatc gcgaaatcga ggcggcgccg
     4501 gggcgctatg tcagtgaacg ttgtgcggga aacgacttca agcatgaagc aggatgaccc
     4561 gaatctttgt ataacgacat tatttacccg gggggaacaa tatggcaaaa caaagaatag
     4621 tcctggtcac tggcggcatg ggcgggctgg gcgaatcgat ttgcaccaaa atggccgatc
     4681 aaggctaccg tgttgtcgcc acttattcat cgaacaaccc caaggccggg caatgggcag
     4741 cggaaatgaa atcgcgggat tacgagttct ataccgcgca agtggatgtg agtgatttcg
     4801 attcctgcgc caggatggtt gcgaaagtgg aaaaggatgt cggccccatc gacgtgctgg
     4861 tcaacaacgc cggcatcacc cgtgacatga cgtttaaaaa aatggccaag gcggattggg
     4921 atgccgtaat caaaaccaat ctggacagcg tgttcaacat gaccaagcag gtgctggacg
     4981 ggatggtgga acgcggctgg ggacgcgtta tcaacgtctc atcggtcaac ggccagaaag
     5041 gcgcgttcgg acaaaccaac tacgccgcgg cgaaagccgg catgcatggt ttcaccaagg
     5101 cgctggcaca ggaagtggcg aaaaaaggcg tcaccatcaa tacgatctct cccggctaca
     5161 tcggcaccaa gatggtgatg gcgataccta aagaggttct ggacagcaaa atcctgccgc
     5221 agataccggt tggaagatta ggcaagccgg aggaagtcgc cgggttgatt atttatcttg
     5281 cttcggacga agcggcattt gtgactggcg ccaacatctc cattaacggc ggccagcaca
     5341 tgtactaaaa aaaaccccgc caggttttgg cggggttaac ctttataata aggaaaacct
     5401 tacgataaag gaggaggatc tgtatcagtg gctcttctta atggttcttc ttggagccgc
     5461 cttttaccgc gctggaggtc gccgagtgcg cggccttgac gccggtatct gcaagttccg
     5521 caacctgcct cgcggccttg gtcatactgt ccaccgcggc ttgagtcgcg gctaccgtgg
     5581 atttcagggc ggcgaccgcc aggtccgaac cggcgggagc ggatttgctg gttttctcaa
     5641 tagcgctcac caagtccttg gtgtaggcgg aaagactttc ttcaaacaac ttggtcagtt
     5701 cagcttgtgc ttgcgacgcg acctcgtaca cgttacggga atagctcaaa gccttttcga
     5761 cacccgcttc ggcaagtttc gtgcgcaaag ctaccacttc ctgcaagtct tttacttcgc
     5821 tcaaggcttt cgtgttcttg gcattctctt caatcgcctg tttggcggtt tcaagctgga
     5881 gcttaaccag gcgttcagcg ctttccatgg tgattctggc aaaccgcagg gcattttcca
     5941 agctgctctt gttgagctcg gggaattgct cgttaaacat ggggaaatcc tttacaaaat
     6001 ttgataatcg gcaagggttt tatgttgcac cgcaataatg ctattctaga ggccatgttt
     6061 agctttgtca atcatttatt gtgcaacgca atataaaggt ttgcggcttt catatttttc
     6121 attcccaaat catgaggtta taattaagtc cgtatttgtc gcaacgaaca taaacctagt
     6181 cagtcgcggc aaagcgcgaa aaaggagcgt gcagcatgaa cgacaccgcc agagttatca
     6241 agaagtaccc gaaccgccgt ctttacgaca cgaccagcag cagctacatt acgctcgccg
     6301 acgtaaagca attggtgctg gatcatgtcg atttccgagt ggtggacgcc aaaactaatg
     6361 aagacctcac gcgcagcatc atgctgcaaa tcattctcga ggaggaaagc ggcggcatgc
     6421 caatgttttc ttccgacatg ctgtcgaaaa tcatccgctt ttacgggcac gccatgcagg
     6481 gcatgatggg agtttatttg gagaaaaata tccagacctt catcgaaatg cagcagcgct
     6541 tgcaggatca atcacacgcc atgtacggcg acaaccccat gctgaatgct gaaacctggg
     6601 cgcagttcct gaaattccag ggtccggcaa tccagggcct gatgacgagc tatctcgagc
     6661 aaagcgccaa aatgttcctc gacatgcaga gccagctgca aaaacagacg cgcaacatct
     6721 tcggcaattt cactttcccc aatttcggtg tcactccaca agagccgccc gccgccggcc
     6781 cgaacaaaga agaagacaaa aaataagcaa atgacattta cttccctgat cgccgcatga
     6841 ccgctgttcc gaaagtcggt tttgtctccc taggctgtcc caaggcgctg gtggattcgg
     6901 agcgcatcct cacccatttg cgcgccgaag gctatctgat tgcgccgagc tatgaggaag
     6961 ccgacctcgt agtagtcaac acctgcggat ttatcgacag cgcggtggaa gaatcgctga
     7021 acgcgattgg cgaagctctt gatgaaaatg gccgcgtcat cgtcaccggc tgcctcggcg
     7081 ccaaagccga cgtggtgcgt cgggctcacc cgcaagtgct ggcggtcacc ggcccgcact
     7141 cggcgcagga agtgatggac gcggtacatg cacatttgcc tcagccgcac gatccctaca
     7201 ccagcctgat tccgccgcaa ggcgtcaagc tcacgcccgc gcattacgcg tacatcaagg
     7261 ttgccgaagg ctgcaaccat cgttgcactt tttgcatcat tccttcgatg cgcggaaatc
     7321 tgatgagccg cccgatcaac aaagtcatgc aggaagcaga aaacctggtt aaagcaggcg
     7381 tgaaagaatt gctggtgatt tcgcaagaca ccagcgccta cggcgtcgac atcaaatacc
     7441 gcaccggctt ctggcgcggc cgcccgctca aaacccgcat ggccgaactt gcgcgcagct
     7501 taagcgagct gggcgtctgg gtcaggctgc actacgttta tccttatccc cacgtcgacg
     7561 aagtgattcc gctgatggcg gaagagaaac tcctgcctta cctggatgtg ccgttccagc
     7621 acgcgagtcc gcgcatactc aaagccatga aacgcccggc aggagcgcaa tgcaatctgg
     7681 cgcgcatccg cgcctggcgc gaagtttgcc ccgacattac gatacgcagc actttcatcg
     7741 tcgggtttcc cggcgaaacc gaagaagatt tcgagcaatt gcttgggttt ctcgaggaag
     7801 cacagcttga tcgcgtcggt tgtttcacct attcgccggt ggacggcgcc gcggcaaacg
     7861 ccctgccggg gcatgttccc gaagaggtga aggaagaaag aaaagcgcgc ttcatgacgc
     7921 tacaggaaaa aatcagcacg gagcgtttga agcgcaaaat cggacggacc atgaccgtgt
     7981 tggtagatga aatcaccggc gatagcgcgg tagcgcgcag ctctgccgat gcgccggaaa
     8041 tcgacggacg agtttacctc aagaattcac gcggacttgt ggctggcgag ctggtaaaag
     8101 tcaaaatcac cgcttccgac gctcacgact tctgggcagc gccgctcaag aattaattca
     8161 cggacaaccc ttgcaattgt cggttagcgc gcccacgaaa ttggcgcctt gcaggttcgc
     8221 cccctgcaaa tccgcgcctt ccagattcgc cccctttaaa atcgccgcgg ccagattcgc
     8281 gcccttcaag tttgcgccct tgagattggc cttgaccagg ttcgccccct gcaaatccgc
     8341 gccttccagg tcggcgccga gcagattagt gcggtacaaa ttgccctcct tgaggttggc
     8401 gcgcttgagg ttcacatgcg acatgctggc ggacgtcagg ttgacgccct gcagattcgc
     8461 gccctgcaac gaggagttgt aaagaaaggc gttttgcaga ttcgtctttt ccaagttcgc
     8521 gccatcgaaa ttggattcgt aaagctgcgc gaattgcagg ttcgcgccgc gcagatcggc
     8581 gttttggcag taggcgttcg gctggagctg gcacatggaa ttctgtgcct gtgcggccgg
     8641 cgacaataac agcaaaccgc agatctgccc cagattgagt agcagcagat ttttgctttt
     8701 catttcgcgc tctccgtttg catgacaggg tggcattgtt cgagaaaatc cacggcctcc
     8761 gtttcctcgc gcgtgcgccg catcggcggc aggctccgcc agattctgcg gccataagac
     8821 ttggacaaca aacgggggtc acaaatcatc agtaccccgc ggtcagtttc atcgcgaatc
     8881 aaacgccccg cgccctgttt caatgttatc accgcccgcg gcaactggta ttccatgaac
     8941 gcattgcgcc cctgccggtt gatttgctcg atccgcgccg ccagcaccgg atcatccggc
     9001 ggcgcgaacg gcagcttgtc tatgatcacc agcgacagcg ccgggccgcg cacatccacc
     9061 ccttcccaga acgactggct gcccaccagc accgcgttgc cgagcctgcg aaagcgttcc
     9121 agcaattcgc tgcgtgagcc ctcgccctgc accagcagcg ggtaatccag ctttgcttcg
     9181 tggaaatatt ccttaagcag ggttcgcgct tcctgcatcg cgcgcaggct ggtgaacagc
     9241 aggaaagcgc ggccgtggct ggctttgatg agaggaagcg ccgctttaat caccgcttgc
     9301 gtatactcgc cgctattggg atcaggcagc gcttgcggca gatacaagag cgcctgctgc
     9361 tcatattcaa acgggctgtc ccaggaagcg gtggccgcgt catgcaatcc catttcgttc
     9421 tggtagtggc tgaaatccct gttcaccgac aaggtcgccg aagtaaaaat cagcgcgcgc
     9481 gcattgcctt cgacctgctt gcggaaaatt tcggcaatcg ataaaggcgt actgttcagt
     9541 tgcattgaat gcgcaaacac ttcgccccag cgcacctgcg tggtgtcctc cgcgtcgcgc
     9601 cagcgcgcaa tttttgccgc aagctcgagg ccgcgctgcc agcaatggcg caggccttcg
     9661 ccgcgctcgg cctggctttc cagcaatccg gtgagcgcat cgagtttttc gcataaccca
     9721 tccagggccg ccttaaaacc ggaaatatcc tgtaatgatt gctgcgacag gcgcgcgttg
     9781 tcttcgttca cggtcagccg caaatccctg gccgctttat ccagagcgga agcggcctcc
     9841 ggcaacaccg tgaaatcgcg cgcattggcg atgccctccg tgcgcgtatc gcgggcaagc
     9901 tccagaatct gcgcggtgct gatgctttcg ccgaaaaaca ggctggcggt ttccggcaat
     9961 tggtgcgcct cgtcgaaaat caccgtgttg cacgccggca gcaattccgc cacgccttcg
    10021 tcgcgcagca tcacgtcggc aaaaaacaaa tgatgattca ccaccaccac atccgcctgc
    10081 tgcgcttctt tgcgcgcttt catcacaaag cattcctgaa aattggggca ttcctggccg
    10141 aggcagtttt cccgcgtgga ggtcacatgc ggccagacgc cggcattttc cggcacttcg
    10201 gccagaccgc ttttgtcgcc tgacgaagaa gtgcgcgtgt attgcgcaat cggcggcaga
    10261 taacccgcgt cttcgcggct gccaaagcgc ccttcattca gcgcctgatt gaggcggtaa
    10321 tggcatatat aattggcgcg tcctttaagc aaggcaatcg tcaccggcgc cttgagcgcg
    10381 gcgcgcacgg tgggaatatc gcgattgaag agctgatcct gcagtgtctt ggtgccggtg
    10441 gaaataatca ccttgccgcc ggagagcaac gccggagcca ggtaagcgaa agtcttgccg
    10501 gtgccggtgc ccgcctcggc gaccagaacc gaattgcctg cgatcgcatc ggcgaccgcg
    10561 agcgccatct cgagctgccc ctggcgcacg cggaagtcgc gcaccgcctg cgcaagcggg
    10621 ccgcggtcgg agaatatcga atcgagattg agcattcagc tatcgtaacc gttttccaag
    10681 aggcgcgccg cacatgatga gcatggtttg tccacgggaa atcccttaag cgccttcgtc
    10741 cagaatgttc taaaatcgct tctacatagt gccttgattc tgatgtagga tgacaaaaac
    10801 cactgcagaa tgtcccaata tttcgggact tttaggtccc ctacttcatg ttaggcccat
    10861 ttccgaattg gagttacgat gaagatcgag ggggtccatc gaattctcag tgcttgagca
    10921 cacggccaat cgtgttgttt cggagatgcc gattcgccca agcatcttaa atccattcgg
    10981 ggttgttcac gctggcgcaa tgctgtggtt cgcagatgtc agcgccactc tgttggtcat
    11041 cggtactgcc acggcctctg aaggcatgaa aggattccca ttggtcataa acctcaatgc
    11101 aaacttcatc ggcaatcaag ccgatggatc cttcaaggcc gtggcctcgt tcgtgaaacg
    11161 cggacgcacg gttagcatcg ttcggaccct cgtctatgga gagggtgaca aactcatcgc
    11221 agacgtctcg accaatcatg tcctttcgaa gtgacgtgat cgggcctaac aatcggttca
    11281 gccgcgtgcc cgctttgtgg gcacgcggct gaacctagat gttagatggc ttgaaagcat
    11341 cttgacgttg tgacgttata acgtcataat accgtcatgg atactcaaaa acgcgctacc
    11401 gtctatttcg agtccgaact ccaccgtgcc ctgcgtttga aagccgcggc tctggatcgg
    11461 tcgatctccg atatggtcaa cgacgcggtg aaagccgccc tcgccgagga cgctgaggac
    11521 cttacggcct tctccgaaag gaagaaagac aggaatcttt ctttcgacac gttcgtccaa
    11581 ggcctgaaac ggcgtggccg aatatagact cctgattaag gcctcggctg caaaggaaat
    11641 cgaggcggta ggcaccaaag ctgatcgcca gcgtatcgtc gagcgtattc gggcgcttgc
    11701 tcaagatccc cgggggcaag gtttggaaaa actcgctggt tcggcgaatc gctaccgggc
    11761 acggcaagga cggtaccgaa tcatttacct catcgatgac gggcgtcgtg aggtcacgat
    11821 cttcaaaatc ggacatcgga aagatgtgta ccgataggcc atccaacaac gcgttcgaag
    11881 gccgacgctc tacaagcgcg gctcaacgcg gacgttagga cgccagaaga gtggaattcg
    11941 atagcctgat cccgctctgg atcaagcttg cctacacgct ttttgtagcg atcacggtgg
    12001 cggtctacgc gaccaagtac agtcgcgaca acttcctttg gttttcggac atcgccttgt
    12061 tggccaccgt ggccgcgctc tggtttgaga gccctttctt ggcaagcatg atggcggttg
    12121 gggtactgct gccggaggcg ctctggaata taagcttctt cgggcagctc ctgaccggga
    12181 agcgcgtgag cgggctgacc gactacatgt tcgacgcacg caagccgctg tacctcaggg
    12241 cactctcgtt gttccacgtg ttcttgcccg cattgctgat ctggatgatt gctcgacttg
    12301 gatatgaacc gggagcctgg attgcgcaga cggcccttgc ctggtttgtg ctcccgctgt
    12361 gttactggct cacggatcct gcagagaacg tgaactgggt gtttggtccc ggaaaccaac
    12421 cgcaaaagcg catggcgcca ctggcttatc tgggtctgct gatggttggc ttccccgtgc
    12481 ttatttatct tcccacgcat ttgctgcttc ggagcctgtt cggataacga ttgatatgtt
    12541 ccgctcgggc gactaggatt ataggcgttt cccttgtcca acccggacac tcgaaggagg
    12601 gtacacatgg ctgccagaaa gaaagcgaaa gccaaagtaa agcggaagaa gacgccggcc
    12661 agaagaccgg caaccctaaa gaagaccgcg caggtcagga aaaaggccgc agggaaaaag
    12721 ccgacggtgc ggcacgtagc gccaaagaaa aagccggcgg cccggccgtc ccccaaggcg
    12781 cgaccggcac gcacgccacc gcggccgacc ggagcggagg catccgctcc cttggcaggg
    12841 gaggagcgcg tgggtaccgt gacccactac tacagccatc tttctgttgc agtggtgcag
    12901 atggaggcag gcagtctgcg cgtcggcgac accattcatg tctggggcca cacgagcgac
    12961 ttccgccaac gggtcgagtc catgcagatc gagcaccaac ccgtgtttga agtcagtgcg
    13021 ggacaggaat tcggcttggg agtgaccgac cacgcgcgcg agcgcgacgt cgtgtacaaa
    13081 gtaaccgctc cgctgcgata aagcccgggg agcaggccta actaattgct ccagcgga
//
LOCUS       DATIOW010000070         4546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_943899, whole genome shotgun sequence.
ACCESSION   DATIOW010000070 DATIOW010000000
VERSION     DATIOW010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943899"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="VLL03_02110"
     CDS             complement(<1..1084)
                     /locus_tag="VLL03_02110"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HSS46194.1"
                     /translation="MADDYQLETLALRAGTHRSQFNEHSEALYLTSSFVFENAAQAAA
                     RFAGDEPGNIYSRFTNPTVTAFQERLAALEGAEACVATASGMSAILACVMGLLKSGDH
                     IIASHSIFGATVQLFTNIFSRFGLDTTFVSATDVSAWQAAVKPNTKILFLETPSNPLT
                     EISDIAALSRVARKAGAWLVVDNCFCTPALQRPLEFGADIIIHSATKYLDGQGRVLGG
                     AVLGKRDVIMDGVFGFLRTAGPTLSAFNAWVLLKGMETLKIRMDAHSANALELARWLE
                     KQPAMEKVYYPGLPSHPQHRLALQQQKTGGGIVSFTVKGGKQAAWAVVDATRMISITA
                     NLGDVKSTITHPASTTHGRIPQEARNA"
     gene            complement(1313..2752)
                     /gene="purF"
                     /locus_tag="VLL03_02115"
                     /pseudo
     CDS             complement(1313..2752)
                     /gene="purF"
                     /locus_tag="VLL03_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004544727.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
     gene            complement(2765..3256)
                     /locus_tag="VLL03_02120"
     CDS             complement(2765..3256)
                     /locus_tag="VLL03_02120"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HSS46195.1"
                     /translation="MTVFDYAVILIIGLSVVLSVIRGAVREVLSLAAWVLAFWAAQYY
                     TVEIATLLPDTLSNPSLRLMTGFALVFLTVLVVMSLIAILSSKLVKASGLSVADRTLG
                     ALFGLARGFLMVLILVLLGGLTSLPKQSMWKEALFSSQLERAATSIKTWLPEGLSKRI
                     NYQ"
     gene            complement(3266..3859)
                     /locus_tag="VLL03_02125"
     CDS             complement(3266..3859)
                     /locus_tag="VLL03_02125"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HSS46196.1"
                     /translation="MARTISDEQLQLKKRARRRLIGAITLVALMVVVLPMLLDGEPKP
                     VSQDINIQIPAQNSGSFASRVIPVPEKTGTAEAAVKPQKTETAAHPPLPPAPKSGADK
                     ETTKTEQGKNEKTATAESKSGAYVVQLAAFAHKDNAKQLQQKLSASGIRSYTEVLKTA
                     RGEKTRVRAGPYESRDAAEKALEQLKALGLNGVVTPK"
     gene            complement(3888..>4546)
                     /locus_tag="VLL03_02130"
     CDS             complement(3888..>4546)
                     /locus_tag="VLL03_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HSS46197.1"
                     /translation="TNIGARLIRINRDFGFIAEKNQWLYWGRSSRRHALPYPALRGAC
                     QLQNASAALAALDELKDKFPVGMGEIRRGLIEVDLSARFQVLPGRPAVVLDVAHNPQA
                     AAALSANLANMGFYRSTFAVFGMLKDKDIAGAARVLKPQVNSWRIASISSERRGATAA
                     DVARELLRAGVPQPALSCFANPGEAYDHACSLAAENDRIIVFGSFHTVAGVLRRRSGE
                     "
BASE COUNT          938 a         1352 c         1255 g         1001 t
ORIGIN      
        1 ccgcgttgcg cgcttcctgg ggaatgcgcc cgtgggtggt tgaagcggga tgggtaatcg
       61 tgcttttcac atcgccgaga ttggctgtga ttgaaatcat gcgcgtagcg tccaccacgg
      121 cccacgccgc ctgcttgccg cctttcaccg taaatgagac gatgccgccg cccgtttttt
      181 gttgctgcaa tgccaagcgg tgctgggggt gcgaaggcaa cccggggtaa tacactttct
      241 ccatcgcagg ttgtttctcc agccagcgcg caagttccag ggcatttgcg gaatgcgcgt
      301 ccattctgat tttcaaggtc tccatgcctt ttaacagcac ccaggcatta aacgcgctta
      361 aagtcggccc ggcggtgcgc agaaaaccaa atacgccgtc catgatgaca tcgcgtttcc
      421 ccaacaccgc gccgcccagc accctgccct gcccgtcgag atacttggtg gcggaatgga
      481 tgatgatgtc ggcgccgaat tccagcggcc gctgcagcgc aggggtgcag aaacaattgt
      541 ccaccaccag ccacgcgccg gccttcctcg caacccggct caacgccgca atatcggata
      601 tttcggtgag tgggttggaa ggcgtctcca gaaaaagaat tttggtattg ggttttaccg
      661 ccgcttgcca agcgctcaca tcggtcgctg acacgaaagt ggtatccaag ccaaaacgcg
      721 agaaaatgtt ggtgaagagc tgcacggtgg caccgaaaat gctatgtgaa gcgatgatgt
      781 gatcgccgct tttgagtaaa cccatgacac aggcgagtat tgccgacatg ccggaagcgg
      841 tggcaacaca ggcctccgcc ccttccagcg cggcaagccg ctcctggaac gcagtgacag
      901 tggggttggt gaaacgtgaa tagatattgc cgggctcgtc acccgcaaaa cgcgccgccg
      961 cctgcgccgc gttttcaaac acgaagctcg aagtgaggta caacgcctcc gagtgctcgt
     1021 tgaactggct gcggtgtgtg ccggcgcgca gcgccagggt ttccagttga taatcgtcag
     1081 ccatagccat cccgtcctta aaaataaaaa acccgttcag cttgcgcgga acgggttgtc
     1141 cctcgcttta gcagcactta taatgcgccc gcaagctgat atcaaatcgg cgcaattgtt
     1201 aagttactat gccggaactt aattgtcaat ccacggtgag cagattcaaa tcgagttggt
     1261 tgctggatgt ggcctcttct ctctgccggc gcgcgccatc gcggcgctgc gcttcgatgt
     1321 gggccaggta ttccggagat atatcaccgg taatgtattc accgctgaaa cacgaggtct
     1381 cgaaatgggt aattttcgga ttcacctgct gcgcagcgtg caccagcgcg tccaagtcct
     1441 ggtaaattaa cttgtctgcg ccgatttctc tggcgatttc gtcgtcgctg cggccgttgg
     1501 caatcagttc ctggcgcgtc ggcatgtcta tgccataaac attgggaaaa cgcaccggcg
     1561 gcgcggccga ggcgaaatac accttgcgtg cgcccgcctc gcgcgccatc tgcacgatct
     1621 tgctgctggt ggtgccgcgt acgatggaat catccaccaa caacacgttc ttgcccctga
     1681 actccacgct gatcgcgtta agtttctggc gcaccgattg ctggcgttgc acctggccgg
     1741 gcatgatgaa agtgcgcccg atatagcggt tcttgatgaa accttcgcga taaggaatac
     1801 cgagacggtt cgcaagctgc atcgcactgg ggcggcttga gtctggtatg ggtatcacca
     1861 cgtctatgtc cagattgctg tggtggcgct ggattttgtc cgccaggctc tcgcccatgt
     1921 gcaggcgggt ttcatatact gaaatgccgt caatcactga atccgggcgc gccaggtaca
     1981 cgtattcgaa aatgcagggg ttgcgcgtag cgtttgccac gcactgtttg ctgtaaaaat
     2041 taccgctgtc gtctatgaat atcgcttcac ccggggcgac atcgcgcgtc agttcaaaac
     2101 ccagggtatc cagcgccacg ctttcggaag cgaccagata ttccgtgccg cgttcactgc
     2161 ggcactgtcc caccaccagc gggcggatgc cgaaggggtc gcgaaacgcc aacagaccat
     2221 aacccgcgat catcgccacc accgcgtaag cgccgcggca gcgtcggtac actcccgaca
     2281 ccgcgttaaa aatggtagcc gcatccaggg cgtgattgcc gcaggattcc tgcaattcgt
     2341 gcgccagcac gttcaacaac acttcggagt cggaattggt attgacgtgg cgcaagtcct
     2401 ggcggaacag ctcgcgcttt aattgctcgg tgttggtgag gttgccgttg tgtccgagca
     2461 cgatgccaaa cggcgaattc acgtaaaacg gctgggcttc ggccggcgaa tcggctgatc
     2521 cggcggtggg gtaacgcaca tgggcgatac ccatgttgcc ctgcagtgcg cgcatgttac
     2581 gggtacggaa cacgtcgcgc accatgccgc tgcccttgtg catgttaaaa gtattgcctt
     2641 gcgccgtgac gatgcccgcc gcatcctggc cgcggtgctg cagcaccagc aggccgtcat
     2701 agagcagctg gttcaccgga gcttttgcca cgacacctaa aattccgcac attccggatt
     2761 tcctctactg gtaattaata cgctttgaca aaccttcggg aagccaggtt ttaatgctgg
     2821 tcgccgctct ttccagttgc gagctgaaca gcgcttcctt ccacatcgac tgcttgggca
     2881 gcgaagtgag gcctcctagc agcaccaata tcagcaccat caaaaagcct cgtgccaggc
     2941 caaataacgc gccaagggtg cggtcggcca cgctcaaacc cgaggctttc actaatttgg
     3001 agcttagaat tgcgatcagg ctcatcacca ccagcactgt caaaaacacc aaggcaaaac
     3061 cagtcatcaa ccgcaacgat gggttgctta aggtatccgg gagcagcgta gcaatttcca
     3121 ctgtgtagta ctgtgccgcc cagaacgcca agacccaggc tgcaagcgac agcacctcac
     3181 gtaccgcgcc gcgaatgaca cttaacacaa ccgacagccc gatgatcaaa attaccgcgt
     3241 aatcaaaaac cgtcatggcg ggtggctact tgggtgtcac taccccgtta agccccagcg
     3301 ccttgagctg ttccagcgct ttttccgcgg catcacggct ttcatagggc ccggcgcgca
     3361 cccgggtctt ttcgccacgc gcagttttca gtacctcggt gtaggaacga atcccgcttg
     3421 cggaaagttt ttgctgcagc tgtttggcat tatctttgtg ggcaaacgct gcgagctgca
     3481 ccacataggc gccggatttg ctttccgctg tggcggtttt ctcgtttttt ccctgttccg
     3541 tcttggtggt ttctttgtct gcaccagatt ttggggcagg cggcagcggt ggatgggccg
     3601 cggtttcggt cttctgcggc ttgacggcag cctcggcagt gccggttttt tcgggaaccg
     3661 ggatcacgcg ggacgcgaaa cttccactat tttgcgccgg aatctgaatg tttatgtcct
     3721 gactcaccgg tttgggttcg ccgtccagaa gcatgggcag caccaccacc atcagcgcga
     3781 ccaaggtaat tgctccgatt aaacgccttc tggcgcgctt tttcagttgc agttgctcgt
     3841 cactgatcgt tcttgccata tacgcaaaat cctcttggca gcaggtttta ctcgccgctg
     3901 cgcctgcgca gcacaccggc tacggtatgg aaagagccga aaacgataat tctatcattt
     3961 tcagccgcca aactacaggc gtgatcatag gcttcacccg gatttgcaaa acacgacagc
     4021 gccggttgcg gcacacctgc ccgcagcaac tcccgcgcca catcggcagc cgtagccccg
     4081 cggcgctcgc tgcttatgct tgctatgcgc cagctgttca cctgcggttt caggacccgt
     4141 gcagcgcctg cgatatcctt gtctttgagc atgccgaaaa cagcgaacgt gctgcggtaa
     4201 aaccccatgt tagccagatt cgccgataac gccgcagcgg cttgcgggtt gtgcgccaca
     4261 tccagaacca ccgcgggcct ccccggcagc acctgaaagc gcgcggacaa atccacttct
     4321 atgagcccgc ggcggatttc tcccatcccc accggaaatt tgtctttaag ctcatccaac
     4381 gcagctaggg cggcgctggc gttttgcaac tggcacgcgc cgcgcaacgc gggataaggc
     4441 agcgcgtgtc gcctgctgct gcgcccccag tatagccact ggtttttttc agcaataaag
     4501 ccaaaatccc ggttgatgcg aatcaatcgt gcgccgatat tcgttg
//
LOCUS       DATIOW010000071         1501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1553873, whole genome shotgun sequence.
ACCESSION   DATIOW010000071 DATIOW010000000
VERSION     DATIOW010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553873"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /gene="hflK"
                     /locus_tag="VLL03_02135"
     CDS             <1..647
                     /gene="hflK"
                     /locus_tag="VLL03_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HSS46198.1"
                     /translation="AIREIVGKSRMDFVLYEGREQVAAHATKLMQQILDRYRTGIQIS
                     KVTMQNAQPPEQVQAAFDDAVKANQDRERQKNEGQAYANDVIPKARGTASRILQEADG
                     YRQRVIANAEGDASRFRQILVEYTKAPGVTRDRLYLDMMQQVLSSTSKVLIDQKSGAN
                     LLYFPLDKLLHIGGPAADAQELAPRAATPEAAQQPESTSRSREAFRSRDREQRQ"
     gene            644..>1501
                     /gene="hflC"
                     /locus_tag="VLL03_02140"
     CDS             644..>1501
                     /gene="hflC"
                     /locus_tag="VLL03_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HSS46199.1"
                     /translation="MKNIGAVLIGIIAVLVVASLGMFTVDQRQNAIVFRLGEIISVKK
                     EPGLYFKVPILDNVRFFETRILTLDSPEPERFLTSEKKNVLVDSFVKWQIADVRQYYI
                     SVGGDEQRAQLRLAQTVNSSLRDEFGNRTVHDVVSGERDKIMNLMREKANEDAGKIGV
                     HVLDVRIKRVDLPQEVSESVYRRMEAERKRVANELRSTGAAESEKIRADADRQREIII
                     AEAYREAQRTKGQGDAKAAAIYGEAYQQNPEFYAFYRSLEAYRQGFKTKNDLLVLDPN
                     SEFFKYLKHA"
BASE COUNT          369 a          427 c          457 g          248 t
ORIGIN      
        1 ccgcgatacg cgaaatcgtg ggaaaaagca gaatggattt cgttctctat gaaggccgcg
       61 agcaggtggc ggctcacgcc accaaactga tgcagcaaat cctcgaccgc taccggacgg
      121 gaatacagat cagcaaagtc accatgcaga acgcgcagcc gccggagcag gtgcaggcgg
      181 cgtttgacga cgcggtgaaa gcaaaccagg accgcgagcg ccaaaaaaac gaaggccagg
      241 cctatgccaa cgatgtgatt cccaaggcgc gcggcacggc gtcgcgcata ttgcaggaag
      301 ccgacggcta ccggcagcgg gtgatcgcca atgccgaagg cgacgccagc cgcttccggc
      361 agatcctggt ggaatacacc aaggcgccgg gggtgacgcg cgaccgcctg tatctggaca
      421 tgatgcagca ggtgctatcc agcaccagca aggtgttgat agaccagaag tccggagcca
      481 atctgctgta cttcccactg gacaagctgc tgcatatcgg cggcccggca gcggatgcgc
      541 aggaacttgc gccgagagct gcgacgcctg aggcggcgca gcagccggaa agcacatcgc
      601 gttcacgcga agcgttccgc agccgcgacc gagagcagcg ccaatgaaaa atatcggcgc
      661 agtactgatc ggcataatcg ccgtgctggt ggtggccagc ctcggaatgt tcaccgtgga
      721 ccagcggcag aacgccatcg tattccgttt gggcgaaatc atctcggtta aaaaagaacc
      781 cgggttgtat ttcaaggttc cgatactcga caacgtgcgc ttcttcgaaa cgcgcatcct
      841 cactttggat tcccccgagc cggagcgttt ccttacctcg gagaagaaaa acgtgctggt
      901 ggattcgttc gtcaaatggc aaatcgcgga tgtcaggcag tattacatca gcgtcggcgg
      961 cgacgagcag cgcgcgcagt tgcggcttgc gcaaacggtg aattccagct tgcgcgacga
     1021 attcggcaac cgcaccgtac atgatgtcgt atccggcgag cgcgacaaaa tcatgaacct
     1081 gatgcgcgaa aaggccaacg aggacgcggg taagatcggc gtgcatgtgc tggatgtgcg
     1141 catcaagcgc gtggacctgc cgcaggaagt gagcgaatcg gtttaccgcc gcatggaagc
     1201 ggaaaggaaa cgcgtcgcta atgaactgcg ttcgaccggc gcggcagaat cggaaaaaat
     1261 ccgcgccgat gccgaccgcc agcgcgaaat tatcatcgcc gaagcctacc gtgaggcaca
     1321 gcgcactaaa ggccagggcg atgccaaagc cgcggcgatc tatggcgagg cctatcagca
     1381 gaacccggag ttttacgctt tttaccgcag cctggaagcc taccggcaag gtttcaagac
     1441 taaaaatgac ctgttggtgc tggaccccaa ttcggagttc tttaaatacc tgaagcacgc
     1501 c
//
LOCUS       DATIOW010000072         8051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1249659, whole genome shotgun sequence.
ACCESSION   DATIOW010000072 DATIOW010000000
VERSION     DATIOW010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8051
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249659"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VLL03_02145"
     CDS             <1..782
                     /locus_tag="VLL03_02145"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSS46200.1"
                     /translation="VPMRIIPVFFVLNFTILSTIVYLLIRFFVLKKEMFQTELGEKNR
                     LLQVEQDKSERLLLNILPKHVAERLKSDEKIIADGLADVTVMFADIVDFTRIAERMLP
                     RHMVDLLNKVFSNFDWLTDKYGLEKIKTIGDAYMVAGGLNDNQTDYVEAVANMALEIR
                     ELVRRHPAFSRQNLDIHIGIATGPVVAGVIGSKRFIYDLWGDTVNIASRITSETVPGT
                     IQVDENTYRRLRDRFSFDAPQTIELKGKGMTTVYQLLPESR"
     gene            complement(789..3482)
                     /gene="glnE"
                     /locus_tag="VLL03_02150"
     CDS             complement(789..3482)
                     /gene="glnE"
                     /locus_tag="VLL03_02150"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HSS46201.1"
                     /translation="MHIPKKPDAASLCEHEQLIARALALSRFARRLIEREPQLKNAVA
                     NGLHRPFTLERMRTFLDDTNDAAGLYRMVRRLRAEVMLHLITRDLCGLASLNEIVATT
                     TALAEATLNVAVKHLYRWMREDRGTPVGKESGKPQELLVVGMGKLGGGELNVSSDIDL
                     VFVYPEEGETSGARPVSNHEFFDRVGRKLIAAMSEITGDGYVFRVDMRLRPYGDGGPL
                     TMSFAMLENYFITQGREWERYAWIKARVVCGGHGEELMKLTRHFVFRKYLDFGVFHSL
                     RNLHAQIRQEVRGRDIENNIKLGPGGIREIEFIAQIFQLIRGGRESRLRIHPTLATLD
                     ELAHLRLLPRDVVNELRDAYVFLRNLEHRLQYLDDTQTQTLPQNAEDQVLIAEAMGFG
                     SYEELLQKLDAHRSRVSLHFEQVLAEPQNDAHHLLPLWQETLAEEEALKQLTALGYQN
                     PQQILERLRGMRQSRRYRQMPAANQSRLDALVPALLEAAAAYGDTALMRILRLMENIV
                     RREAYLALLTEHPQALQNLARLCGASSWAAEYLTCHPLVLDELLDPRTLYAEPDWPRL
                     KSHLHAQLKDADTEHQVDILRQFHHSLVFRLLTQDLAGLLALERLSDHLSDLADSILE
                     EALRLCWEGLDNNQAPRFAIIGYGKLGGKELGYATDLDLVFLYDDERAEAQEVYARLT
                     QRINTWLTSYTAVGILYDTDLRLRPDGASGLLVSSLAAFAEYQERRAWLWEHQALTRA
                     RFVAGDRQIGERFEALRKQVLRQKRDLAELKNEVLNMRQKMLEAHSNAGTLFDLKHDR
                     GGIIDVEFIVQYLVLGFSHAHPELTGNIGNLALLKLGAKLKLLPAALAEEVCTAYREF
                     RRMQHALRLDGEKYARVEVEKVRGKREAVLQLCRCVFGNPP"
     gene            3635..7447
                     /locus_tag="VLL03_02155"
     CDS             3635..7447
                     /locus_tag="VLL03_02155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HSS46202.1"
                     /translation="MNGPLSWIKPVKRLLFSLFMFALLALAAVVLGLRYWILPNIENY
                     SEDIAQAASTLIGQRITIGKIAADWDGHRPHLVLDDVQVFDPNDRPALVLNRVETTLS
                     WESLLAGGVRLYSLEFKQPNLSIRRDTEGVVHVAGVALSRHDADQGFTDWLLKQDHLV
                     IRDALVLWNDEKLGAPQLVLNSVMLRIENSGDRHRLSLRASPPPDLAAPLEIEGDFSD
                     DSLDPDDLDKWQGTLFAQVDYADLVAWRTWLPYPIEMQKGSGAMRLRLTVGDAQIKEI
                     TADVQLSQVKTRLRPDLPELDLDSLNGSLGWKMLDHGFELSAAKLSLAAQGGLSLKPA
                     DFVVRIYPAAEGQALRGEFEGGALDLEPLAAMADRLPLNPELRQRLASLAPKGSVYDV
                     VLKWNGDLPKPESYSVKGRFERLALKAYEAWPGFNNVSGSVEGNQQAGQINLEAKNIK
                     LGLPKVFEEALQFESFDARLDWKTENSGTQFSLSNMVFANAHLSGSASGSYRKVSEGP
                     GIVDLNAHVTRVEAKQVNRYLPLQVPQKTREWLVTALGGGAFNDLQLTMRGDLADFPF
                     ADESKGLFQVTARATGISLGYSPEWPKIENITAAMRFRGKRVEIRGSEAEILGIKLSQ
                     IDAVIPELGDHQEVLQIDVEAQAQAANFLNFIGHSPVSSMIGGFTENVHASGDGKLLL
                     KLEISLHQREQTKVAGTYQFHDNQLSFGPDLPPIEQVKGRLQFSEAAMQAQDLSAQIL
                     GGTASVSIATQPDRSVRIEAHGNVNAAAWGSNVALPFQSYLRGSAEWRGSINLSNKVN
                     DMLFESNLEGLASLLPAPFNKSAQASVPLRFEKKSTSSGHERVLLSYGNVLSMRVVRH
                     KDGLDYERGLIVLGGRAGVPDKNGIWLSGALKSFDFDQWHTVLAQSDSGLRLDLAGIE
                     MKLGTLDVFGRRFGETRVSVRHQGGDWKATLAGAEVNGELNWYPQDKGKLVARLANLV
                     IPEPLPETSSAAPQESEQRDFPALDIVVENFNLADKPLGRLELQAVQQGRDWRIDNLR
                     LSNADSTLALEGSWLNFSAQPQTRVNVKLEVIDIGNLMARLKLPEGIKHGSAKLEGAL
                     SWDGSPQRIDYATLSGNLQLNAEKGQFVKLDTGVAKLLGILSLQALPRRALLDFRDIF
                     SKGFAFDDITSSLNLDQGVATTEDFLMSGPAATVNMSGAVDLAQETQNLKVKVAPSFI
                     DSLALAGFLGGPIIGITAFLVQKALKDPLGQMIVYEYDVTGTWTDPVVIKPDSSSPLH
                     DIPFLQNH"
     gene            7447..7797
                     /locus_tag="VLL03_02160"
     CDS             7447..7797
                     /locus_tag="VLL03_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46203.1"
                     /translation="MFYDVIKLAAALCCRCSVPALHQPPTFIQYRRNYGTGRAAGALF
                     WNRKTCARPSAAYARQPDSLMVIHHGNAQQTQLQATELRSWLTSLAVDSARILLLADL
                     PANDGLSIEIRMTK"
     gene            7794..>8051
                     /locus_tag="VLL03_02165"
     CDS             7794..>8051
                     /locus_tag="VLL03_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HSS46204.1"
                     /translation="MSVKTRSTSLKKTSAAAGNVYRIAAVQMASGPNVKGNLREAARL
                     IAMAAEQGAMLVALPEHFAIMGMKDRDKVAVREAEGKGPIQE"
BASE COUNT         1727 a         2245 c         2385 g         1694 t
ORIGIN      
        1 gcgtaccgat gagaatcatc ccggtgtttt ttgttctgaa tttcaccata ctttcgacca
       61 ttgtttattt gctgatccga ttcttcgtcc tgaaaaaaga aatgtttcag accgagctcg
      121 gagagaaaaa tcggctgttg caagtggagc aggataaatc ggaacgcttg ttgctcaaca
      181 tcctgccgaa acacgttgcc gaacgcctga agagcgatga aaaaatcatc gccgacggcc
      241 tggccgatgt cacggtaatg tttgccgaca ttgttgattt tacccggatt gccgagcgca
      301 tgctgccgcg gcacatggtc gacctgctca acaaggtgtt ttccaatttc gactggctga
      361 cggataaata cgggctggag aaaatcaaga ccatcggcga cgcctacatg gtggcgggcg
      421 gcctgaacga taatcagacc gattatgtgg aagcggtggc caacatggcg ctggaaatcc
      481 gcgagctggt gcgccggcat ccggcgttca gccgccagaa cctcgatatt cacattggca
      541 tcgccaccgg cccggtagtg gcgggtgtca tcggcagcaa gcgctttatt tacgacctgt
      601 ggggcgacac ggtgaacata gcgagccgca ttacttcaga gaccgttccc ggcaccatcc
      661 aggttgatga gaatacctac cgacgtctgc gcgaccgctt ttcgtttgat gcgccgcaga
      721 ccatcgaact caaaggcaag ggtatgacta ccgtctacca gctcttgccg gaaagtagat
      781 aaacggcatt acggcgggtt gccaaacacg caccgacaca gttgcaagac tgcctctcgc
      841 tttcccctta ccttttccac ttccacccgg gcgtattttt cgccatccaa tcgcaaggca
      901 tgctgcatcc ggcggaattc gcgataagcg gtacatacct cttcagcgag cgcggcgggc
      961 agcaacttga gcttcgcccc aagcttaagc aacgccagat taccgatatt gccggtgagt
     1021 tcgggatggg catgggaaaa ccccagcacc aggtattgga cgatgaattc cacgtcaatg
     1081 atgccgccgc ggtcatgctt gaggtcgaac agcgttccgg cgttggaatg tgcctcgagc
     1141 attttctggc gcatgttgag aacttcgttc tttaattccg caaggtcgcg cttctggcgc
     1201 agcacctgtt tgcgcaaagc ctcgaaacgc tcgccaatct gccggtcacc ggcgacgaag
     1261 cgcgcccgcg tcaatgcttg gtgctcccac agccaggcgc gtcgttcctg gtattcggca
     1321 aaagcggcaa ggctgctcac cagcaggcca ctggcgccat ccgggcgcaa acgcaaatca
     1381 gtgtcgtaga gaatcccgac ggcggtgtag ctggtaagcc aggtgttgat gcgctgagtc
     1441 agtctggcgt acacttcctg ggcttccgca cgctcatcgt cgtaaagaaa caccaggtcg
     1501 aggtcggtcg cgtagccgag ctctttgccg ccaagcttgc cgtagccaat gatggcaaaa
     1561 cgcggcgcct ggttgttgtc caggccttcc cagcacaggc gcaatgcttc ctccaatatt
     1621 gaatcggcga gatcgctcaa atgatcactc aggcgctcga gcgccagcaa gccggcaagg
     1681 tcctgggtta gcagacgaaa taccagtgaa tgatgaaact gccgcagaat atccacttgg
     1741 tgctcggtat cggcgtcctt gagttgcgcg tgaagatgcg atttgaggcg tggccaatcc
     1801 ggctcggcgt acagagttcg cggatcgagc aattcatcca gcaccagcgg atggcacgtg
     1861 agatattcgg ccgcccagga acttgcgccg cacagccgcg ccaggttttg cagcgcctgc
     1921 ggatgttcag tcagcaacgc cagataggct tcacggcgga cgatgttttc catcagccgc
     1981 aggatgcgca ttagcgccgt gtcgccgtaa gccgcagccg cttcaagcag cgccggcact
     2041 aacgcatcga gtcgcgactg gttggcggcc ggcatctgcc ggtaacggcg gctctgacgc
     2101 atgcctcgca agcgctccaa gatctgttgc gggttctggt aacccagcgc cgtgagctgc
     2161 ttgagcgcct cttcctcggc gagcgtttct tgccacaacg gcaaaaggtg atgcgcgtcg
     2221 ttctgtggtt cggccagtac ctgctcgaaa tgcaggctga cgcggctgcg gtgcgcatcg
     2281 agtttttgca acaactcttc ataactgccg aagcccatcg cctcggcaat caacacctgg
     2341 tcttcggcgt tctgcggcag cgtctgggtt tgcgtgtcgt cgagatactg cagacgatgc
     2401 tccaaattgc gcaggaacac gtaagcgtct cgcagttcgt tgaccacgtc ccgcggcagc
     2461 agccgcagat gcgcgagctc atccagcgtc gccagcgtgg ggtggatgcg caggcggctt
     2521 tcgcgtccgc cgcgaatcaa ctggaaaatc tgggcgatga attcgatttc gcgaatcccg
     2581 cccggtccca gcttgatgtt gttttcgata tcgcgccccc gcacttcctg gcggatttgc
     2641 gcgtgcagat tgcgcagcga atggaagacg ccgaaatcga ggtatttacg gaacacgaag
     2701 tggcgcgtga gcttcattaa ttcttcaccg tggccgccgc acaccactcg cgccttgatc
     2761 caggcatagc gttcccattc gcgcccttgg gtgatgaagt aattctccag catagcgaag
     2821 ctcatcgtca gcgggccgcc gtccccataa gggcgcagcc gcatgtccac gcgaaacaca
     2881 tagccatccc cggtgatttc actcattgcg gcaatcagct tgcgtcccac ccggtcgaag
     2941 aattcatggt tgctgacggg gcgcgctccg ctggtttcgc cttcctccgg atagacgaag
     3001 acaagatcaa tgtcggaaga cacgttgagt tcgccgccgc cgagtttccc cattcccacc
     3061 accagcaatt cctgcggttt gccgctttcc ttgccgacgg gtgtgccgcg gtcttcccgc
     3121 atccagcgat acagatgctt gactgcgaca ttcagcgttg cctcggcgag ggcggtggtg
     3181 gtggcgacaa tttcgttaag ggaggccagg ccacacaaat cgcgcgtgat gagatgcaac
     3241 atgacttccg cccgcaaacg gcggaccatc cggtaaaggc ctgccgcgtc gtttgtgtca
     3301 tccagaaacg tgcgcatccg ttctagcgtg aaaggccggt gcaggccgtt tgcgacggca
     3361 ttcttgagct gcggctcgcg ttcaatcagc cgccgggcaa aacggctcaa agccagtgcc
     3421 cgcgcgatca gttgttcatg ctcgcaaagt gaggcggcat caggtttttt gggtatatgc
     3481 atgggggaat cgcgttacaa tgcgggttaa attgcctgtt gtttagaacg atgcgggcac
     3541 attgcaaccg agttgtcatt ttattgtcta gagcctatct caacaatacc gcgagagacg
     3601 ttgccggcca cagagccttg gtaaattctg aatcatgaat ggcccattat cctggataaa
     3661 gccggttaag cggctcctgt tttcgctttt catgtttgcg ctcctggccc tggcagcggt
     3721 ggtgctgggc ttgcgctatt ggatcctgcc caacattgaa aactacagcg aggatattgc
     3781 gcaggccgcg agcaccctta tcggccagcg cattaccatc ggcaagatcg ccgcggactg
     3841 ggacggccac cgtccgcatc tggtgctcga cgacgtgcaa gtcttcgacc caaacgaccg
     3901 cccggcgttg gtgttgaacc gggtcgagac tacgctgtcc tgggagtctt tgctcgccgg
     3961 cggcgtgcgc ctttattcgc tggaattcaa acagcccaat ctgagcatac gccgtgatac
     4021 tgaaggcgtc gtgcatgtag ccggcgtggc gttaagccgg cacgacgcag accaggggtt
     4081 taccgactgg ctgctcaagc aggaccacct agtcatccgc gacgcgttgg ttctgtggaa
     4141 tgacgagaaa ctcggagcac cgcaactggt attaaacagc gtcatgctac gcattgaaaa
     4201 cagcggcgat cgccaccgcc tgagtttgcg cgcatcaccg ccgccggatc tcgccgcgcc
     4261 gctggaaatc gaaggggact tcagcgacga cagcctcgat ccggacgatt tagacaagtg
     4321 gcagggcacg ctgtttgcgc aagtggatta cgccgatctg gtggcatggc gcacttggct
     4381 gccctatccc atcgagatgc aaaagggcag cggcgcaatg cgcctgcggc tcaccgtcgg
     4441 cgacgcgcaa atcaaggaaa tcaccgcgga cgtacaatta agccaggtga aaacacggtt
     4501 gcgtcccgat ctgccggagc ttgatcttga ctcgctcaac ggcagtctgg gctggaaaat
     4561 gctggatcac ggttttgagc tctcggcggc gaaattaagc ttagcggctc aaggcgggtt
     4621 gagcttgaag cctgcggatt tcgtggtgcg gatttatcct gcggccgagg gtcaggcgct
     4681 gcgcggcgaa tttgaaggcg gggcgctcga tctggaaccg ctggcggcga tggccgatcg
     4741 cctgccgctc aacccggaat tgcggcagcg gcttgccagc cttgcgccga aaggcagcgt
     4801 ttacgatgtg gtactgaaat ggaacggaga tctgccgaaa cctgagagtt acagcgttaa
     4861 aggcaggttt gagcgtctcg cgcttaaggc ttacgaagcc tggccgggtt tcaacaatgt
     4921 cagtggcagc gttgaaggca atcaacaggc cggccaaatc aacctggaag caaaaaacat
     4981 caagcttggt ctgccgaagg tatttgaaga ggcgctgcaa tttgaaagct tcgacgcccg
     5041 gttagactgg aagacagaaa acagcggcac gcagttcagc ttgagtaata tggtgtttgc
     5101 taatgcacac ctttccggca gcgccagcgg cagctaccgg aaggtaagcg aaggccccgg
     5161 catcgtagat ttgaacgccc atgtgactcg cgtggaagcg aaacaggtta accgctattt
     5221 acctttgcag gtgccgcaaa agacccgcga gtggctggtt acggctttgg gtggcggcgc
     5281 attcaacgat ttacagctca ctatgcgtgg cgacctcgcc gattttcctt ttgccgacga
     5341 gagcaaaggc ttgttccagg tcacggccag ggcaaccggc atttcgctcg ggtattctcc
     5401 ggagtggccg aaaatagaaa acatcaccgc cgcaatgcgg ttccgcggca aacgcgtgga
     5461 aatccgtgga agcgaagcgg agattctcgg catcaagctt tcccagattg acgcggtgat
     5521 ccccgaactc ggcgatcatc aagaggtgtt gcaaatcgac gtcgaggccc aggcccaggc
     5581 agcgaatttt ttgaatttca tcggccattc cccggtcagc agcatgattg gcggttttac
     5641 cgaaaacgta catgccagcg gcgacggaaa attgctcttg aagctcgaga tctccttgca
     5701 tcagcgtgag caaaccaagg tcgcggggac ttaccagttt cacgacaacc agttgtcgtt
     5761 cggccccgat ctgccgccga ttgaacaagt aaaaggcaga ctgcaatttt ccgaggcggc
     5821 gatgcaggcg caggatttga gcgcacagat tcttggtggg accgccagtg tgagcattgc
     5881 cacccaacca gatcgcagcg tgcgcattga ggcgcacggc aatgtgaatg cggcggcatg
     5941 gggaagtaac gtggccttgc cgtttcagtc ttacctgcgc ggctccgccg aatggcgcgg
     6001 cagcataaac ttaagcaata aagtcaatga tatgctgttc gaatcgaatt tggaggggct
     6061 ggcttcgctg cttccggcac ctttcaacaa gtcggcacag gcttcggtgc cactgcggtt
     6121 tgagaagaaa tccacctctt caggccatga gcgggtgtta ttgagctacg gcaatgtgct
     6181 ttccatgcgg gtggtgcggc acaaggacgg gctggactat gagcgtggcc tgatcgtatt
     6241 gggcggacgt gccggtgtcc cggacaaaaa cggcatttgg ttgagcgggg ccctcaagag
     6301 ttttgatttt gatcagtggc acacagtgct cgcccaatcc gattcgggcc tgcgtctgga
     6361 tcttgccgga attgagatga aattgggcac gctggatgtc tttggccgcc gcttcggcga
     6421 aacccgggtg agcgtccgtc atcagggcgg ggactggaaa gcgacgcttg ccggcgccga
     6481 ggtgaacggc gaacttaatt ggtatcccca ggacaagggc aagttagtgg cgcgtcttgc
     6541 caatctcgtt atccccgagc cgctgccgga aacaagcagc gcggccccgc aggaaagcga
     6601 gcaaagagat tttcccgcgc ttgatattgt tgtggagaat ttcaatcttg ctgacaaacc
     6661 tctgggacga ctggagctgc aagcggtgca gcagggccgc gactggcgca tcgataacct
     6721 gcggctttcc aacgccgaca gcacgctggc gctggagggg tcatggctca acttcagcgc
     6781 ccagccgcag acccgggtca atgtcaagct ggaagtgatc gatatcggca atttgatggc
     6841 gcggctcaag ctcccggaag gcatcaagca cggaagcgcg aaactggagg gtgctttgtc
     6901 atgggacggc agtccccagc gcatcgatta tgcgacacta tcgggaaatt tgcagttgaa
     6961 cgccgaaaaa gggcagtttg taaagctcga cacgggtgta gccaagctgc tcggcattct
     7021 gagcttgcag gcgttgccgc gacgcgcatt gctggatttt cgcgacatct tcagcaaagg
     7081 ctttgccttc gatgacatca cctccagtct taatcttgac cagggcgtgg ccacgactga
     7141 agatttcctt atgagcggcc cggcggcgac ggtgaacatg agcggagcgg tggatttggc
     7201 ccaggaaacg cagaatctta aggtgaaagt agcgccgagt tttatcgata gcctggcgtt
     7261 agcgggattc ttgggcggtc ccatcatcgg cattaccgct tttctggtgc agaaagcgct
     7321 taaggatccg ctggggcaga tgattgtcta cgaatacgat gtaaccggaa cctggactga
     7381 tccagtggtg ataaagcctg acagttcttc tccactacac gatatacctt ttttgcagaa
     7441 ccactgatgt tttatgacgt tatcaaactt gccgcggctt tatgttgccg atgctcggtt
     7501 ccggccctgc accagccgcc gacttttatt cagtaccgca ggaattatgg gaccggtcgc
     7561 gcagcgggcg cgctgttctg gaacaggaaa acctgcgcca ggccatccgc cgcgtatgcc
     7621 aggcaaccgg actctttgat ggtcattcac cacggcaacg cccagcaaac gcagttgcag
     7681 gcgacggaac tgcgctcatg gcttaccagc cttgcggtgg attctgcccg cattttgctg
     7741 ctggccgatt tgccggcgaa tgacgggctc agcatcgaaa tcaggatgac aaaatgagcg
     7801 tcaaaacccg ttccacctcc ttgaaaaaaa cttccgccgc ggcgggcaac gtgtatcgta
     7861 tcgccgcagt gcaaatggcc tcaggaccga acgtcaaagg gaacctgcgc gaagcggcgc
     7921 ggctcatcgc catggcggcg gaacagggcg cgatgctggt ggcgctgccc gaacactttg
     7981 ctatcatggg tatgaaggac cgcgacaagg tggcggtgcg ggaagcggaa ggcaaggggc
     8041 cgattcagga a
//
LOCUS       DATIOW010000073         7593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_436651, whole genome shotgun sequence.
ACCESSION   DATIOW010000073 DATIOW010000000
VERSION     DATIOW010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7593
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436651"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VLL03_02170"
     CDS             complement(<1..603)
                     /locus_tag="VLL03_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HSS46205.1"
                     /translation="MNGWIIFFLLLALMLTGMPISIALGLTVLAFLFTMTKIPIEIVS
                     QRLFTGLDSFAIMAIPFFILAGNFLITGGVARRMIRFAMALVGHLHGGLAMAGVFACA
                     LFAAVSGSSPATVVAIGSILIPAAVRAGYPKRFGVGAIGTAGALGILIPPSIVMVIYS
                     VSTNTSVGKLFIAGIIPGILLAALLMGITWIIAKRNNYPRM"
     gene            complement(600..1160)
                     /locus_tag="VLL03_02175"
     CDS             complement(600..1160)
                     /locus_tag="VLL03_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HSS46206.1"
                     /translation="MFNRILDHLEEWLIATFIAVATIVIFLAVLHRYASGVSWLWGIT
                     QWINVTWAQELCIYLFIWMAKFGAAYGVRTGIHVGVDVVINHLSEPKRRFWVLFGLLA
                     GAFFTASIAILGSRFVYYIYLTGQVSPDLEMPMWIIYLAIPLGSSLMCYRFLQVAWKF
                     HRTGELPKHEVARVEGIEPLVQKGAA"
     gene            complement(1246..2255)
                     /locus_tag="VLL03_02180"
                     /pseudo
     CDS             complement(1246..2255)
                     /locus_tag="VLL03_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451430.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2258..3679)
                     /gene="tilS"
                     /locus_tag="VLL03_02185"
     CDS             complement(2258..3679)
                     /gene="tilS"
                     /locus_tag="VLL03_02185"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HSS46207.1"
                     /translation="MASSKKSPRCDLRSHVAGVLAKVLKSNNPHLALGLSGGVDSMVL
                     LDILQALSKKLRFKLSAVHVNHQLSPNASSWARFCERQCRACGVTLRMAKVKVSGARE
                     IGLEAAARQARYRVYCKQPADYIVLAHHLDDQAETLLLQLLRGSDVKGSSAMPDIRIV
                     DSGFETRKAGLCILRPLLNVPRSEIERYAKSRKLKWIEDESNDNIRLDRNFLRHKVFP
                     VIGKHFPAYRETLGRAGRHFAEAAQLLDELAGLDLRTSNRDGRLQVAALRKLTPARAK
                     NLLRHYLAQNNVPVPGAKKLDEILHQLTGARGDARVSMVLGEWEVRRFKGEVYICGVQ
                     RDAGELCIRWRGEPCLSISELNGVMSFQRGKGKGIAYARLAAKLVTIRLRQGGEKLRP
                     DGRRPRRSLKNLLQESAIAPWERSRLPLLFSGRRLVFVPGIGIDSDYQAKPGEPGLSV
                     KWDVNYTGSSSNKVHKLKNNYGE"
     gene            complement(3648..4616)
                     /locus_tag="VLL03_02190"
     CDS             complement(3648..4616)
                     /locus_tag="VLL03_02190"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HSS46208.1"
                     /translation="MKTTFLDFEQPIAELEAKIEELRYVQDDSALDISEEINRLQKKS
                     QALTKEIYSKLTAWQISQVARHPQRPYTLDYVQNLFTDFEELHGDRRFADDAAIVGGL
                     ARFNGQTVMAIGHQKGRDTKEKIRRNFGMPRPEGYRKALRLIKTAEKYGVPVLTFIDT
                     PGAYPGVGAEERGQSEAIGRNLYVMSELRVPIVCAVIGEGGSGGALAIAVGDTILMLQ
                     YATYSVISPEGCASILWKSADKAPDAAETLGITATRLKTLGLVDKIINEPLGGAHRNH
                     EEMMQLMKKALQDTLKQLQDKSLDELLRARFERLMGYGKFKEIAPL"
     gene            complement(4677..6386)
                     /gene="ggt"
                     /locus_tag="VLL03_02195"
     CDS             complement(4677..6386)
                     /gene="ggt"
                     /locus_tag="VLL03_02195"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HSS46209.1"
                     /translation="MAQFIESPGLMRRCRFIVLWSVLLPLAVFPAAPPEHFSNAAIAC
                     AHPLATQAGYQILKKGGNAFDAAVAVAAVLAVVEPYSSGLGGGGFWLLHRANDGKEVM
                     LDARETAPGKAAPSLYLDERGEVISKAALRGPIAAAIPGTPAALDYLARHYGRLPLKV
                     SLAAAIGYARSGFAVDARYAALALRFQSQLTGEGRRLLLDYGSVPQAGFVLKQKGLAA
                     TLQAIASKGDDGFYQGRVAQEMVAAVQAGGGIWELDDLKNYRVIERRPIQFAYRSLKI
                     TAASLPSAGGLTLAQSLNILSVLPFETATAETQAHFVAEALRRAYQDRARYLGDADFV
                     SVPVEQLLSKDYAASRAVTIDPEHATPSVKLGEIPDAIGEGADTTHFSIVDRQGNRVA
                     ATLSINTPFGSGFVAGNTGVLLNNEMDDFSVAPGVPNAYRLQGFHGNEIAPGKRPLSS
                     MSPTFVEDERGILILGTPGGSRIISMLLLALLDYAGAPRFDLDAMLGLPRYHHQFLPD
                     RIEIEPRGFSEEWIRKLQAKGHVISKSGRRWGNMQAVYINKLKGRAEAASDPRGQGGG
                     LGW"
     gene            complement(6370..>7593)
                     /locus_tag="VLL03_02200"
     CDS             complement(6370..>7593)
                     /locus_tag="VLL03_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HSS46210.1"
                     /translation="RIIEDILTIIAYAAWGLVQLRYVGLDLGGIIATSAVITGVVAFS
                     MQDTLGNVLGGLALQLDNSIRPGDWIKVDDLVGKVMEIRWRSTTIETRNWETVVFPNS
                     QLMKNKFTVLGRKSGQPEQWRRSILFSIDIGVAPSQVISVVGLAINQADIAHVAKQPP
                     ASCILMEFESGSGRYALRYWLTDLLYDDVTDSAVRAHIYAALQRVGLRFALSEQSVRV
                     IKQDEQREQMARSRELNRRFAALKNVDLFEDFSDEELRTLAQRLKYAPFARGEVMTRQ
                     GAIAHWLYIMTSGEAEVALDTPEGEKLILDTLPAGSFFGEMGLMTGDPRRASVIAKTD
                     VECYRLDKSAFEDILRARPDIAGEISQILATRRVALERIRQNMSAEKSAAEIAQQRGE
                     ILEKIKRFFGIGAVH"
BASE COUNT         1620 a         2290 c         2088 g         1595 t
ORIGIN      
        1 catgcgcggg taattgttgc gcttggcgat gatccaggtg atgcccatca acagcgcggc
       61 gaggagaatg cccggaataa tgcctgcaat gaaaagtttg ccgacgctag tattagtgct
      121 tactgaatag atcaccatca cgattgaagg cggaatcagt atgccgagcg cccccgcggt
      181 accaatggca ccgacgccga agcgtttcgg atagccagcc cgcacagccg caggaatcag
      241 gatggaacca atcgcgacga cggtcgccgg gctggagccg gacaccgctg cgaacagcgc
      301 gcaggcgaac acgcccgcca tggcgaggcc gccgtgcagg tggccgacga gcgccatggc
      361 gaaacggatc atgcgccgtg ccaccccgcc ggttatcaga aagttgccgg caaggatgaa
      421 aaaaggtatt gccatgatgg cgaacgaatc gagacctgta aaaagccgct gcgacacgat
      481 ttcgatgggt atcttggtca tggtgaacaa gaacgcaagc acagtcagac cgagcgcgat
      541 cgagatcggc atgccggtaa gcatgagcgc caagagcagg aaaaagataa tccaaccgtt
      601 catgctgccc ctttctgcac aagcggctcg ataccttcga cacgcgccac ctcatgcttt
      661 ggcagctcgc ccgtgcggtg aaatttccac gcaacctgca ggaagcggta gcacatcagc
      721 gaagagccga gagggatggc gagataaata atccacatcg gcatttccaa atcgggcgag
      781 acctggccgg tgagataaat gtaataaaca aacctggagc cgagaatggc gatggaggcg
      841 gtgaagaatg cgcccgccag cagtccgaac agaacccaga accgccgctt cggctccgaa
      901 aggtggttaa tcaccacgtc caccccgacg tgaatgccgg tgcgcacgcc gtacgccgcg
      961 ccgaatttcg ccatccagat gaaaagataa atgcagagct cctgtgccca cgtgacgttg
     1021 atccattgtg tgatccccca cagccaggac accccagacg catagcgatg taggacggca
     1081 aggaagatga cgatggtggc tacagcgatg aaggtggcga tgagccactc ttcaagatga
     1141 tcaaggatgc ggttaaacat cacgattcag gattcaggag attccttaac aggaccctcg
     1201 acagccgatt cccactcccg aattccggct tctgaatcct gctttttatt ttttccgttc
     1261 tttttcgact tgtgcggcga tattgtaaat ggcgtaaacc agatctttgc cgattgtgtt
     1321 ttcaaactgg gtatgcaccg gcagcaacgc tttttgccag gccagccggt ctttgtctgg
     1381 cagaacataa acctcggtgg ttttttccgc acgcacggcg gcgagcgcat cgtcattttc
     1441 cttctgggcg atttcacgct cgtatttggt ggtttccttc attgcctgct caagtgtggc
     1501 gcggatatcg gcgggcaagc cttcccaaaa tttcttgttg gtgatgaccg cgtagccgag
     1561 atagccgtga tcggaaatcg tcagatattt ctgcacctcg tgcattttct gggtgtatag
     1621 gtttgaaacc ggattctcgg tgccgtccac caccccctgc tgcaaggcgg tatagacttc
     1681 gctgaaggcc atcacctgag ggttggcgcc taatgcgcgc atctgcgctt ccagcacctt
     1741 ggaagactgg atgcgcattt tcagaccctt gaaatcctcg acggccttga gcggcttgtt
     1801 ggcgctcatc tgcttgaagc cgttgtccca gtaagcaagg ccgatgatgc ctttcggctc
     1861 gagtttttga gaagaccctg gccgatttcg ccatccatca cgcgataaag cgtttccttg
     1921 ctgggaaaca tgtacggtaa gtcgtacacc tcgaattcac gcacgcccag cggcccgaac
     1981 ttggcgagag aaggcgcaag catgtgcact gcgcccagct gcagcgcttc catctcttcg
     2041 cggtccttgt agagctggct gttaggatag acttcgactt tgacgcgccc gttggtgcgc
     2101 tcttccacaa gctgcttaaa tctcaacgag gccttgcctt tgggcgtatc tggggctacc
     2161 acgtggctga atttgatgat gatcgggtcg tcagcttgag caggggcagc aaaccccagc
     2221 gcgcacaagc caagaagtat ggaagagagt ctcattgtca ttctccgtag ttgttcttca
     2281 gtttatgaac tttattgctt gagctaccag tatagttcac gtcccattta acagacaatc
     2341 ccggctcccc cggtttggcc tgatagtccg agtctatgcc aatgcctggc acaaacacca
     2401 aacgcctgcc gctgaagagc agcggcaaac gcgagcgctc ccagggcgcg attgccgatt
     2461 cttgcagcaa atttttcagg ctgcgccgcg gccgcctgcc atccgggcgc agcttctctc
     2521 cgccttgcct caggcgaatg gtgactagct ttgccgccag ccgggcgtaa gcaatgcctt
     2581 tccccttgcc gcgttggaac gacattacgc cattgagctc gctgatggac aggcaaggct
     2641 cgcctcgcca tcgaatacaa agctcacccg cgtcgcgttg cacgccgcag atataaactt
     2701 cgcccttgaa acgccgcact tcccattcgc ccaaaaccat gctcaccctt gcatcgccgc
     2761 gggccccggt aagctggtgc agaatttcat ccagcttttt tgcgcccggc accggcacat
     2821 tattttgggc aagatagtgg cgcagcaaat tcttcgcccg cgccggcgta agcttgcgca
     2881 gcgctgccac ctgcagcctg ccgtcacgat tcgacgtgcg caaatccagc ccggccagtt
     2941 catccaacaa ttgagccgct tcggcaaaat gccggccagc tcttcccagc gtttcacgat
     3001 aagcgggaaa atgttttcca attacgggaa acaccttgtg ccgcaggaaa ttgcggtcaa
     3061 ggcggatatt gtcgttgctt tcgtcttcaa tccatttcag ttttcgcgat ttggcatagc
     3121 gctcgatttc ggagcgcggc acgttcagta gaggtcgaag gatgcaaagc ccggctttgc
     3181 gagtctcgaa tcccgaatcc acaatcctga tgtccggcat cgcgctcgag cccttgacgt
     3241 cgctgccgcg cagcaattgc agcagcaagg tctccgcctg gtcgtccaga tgatgcgcca
     3301 gcacgatgta atccgcaggc tgcttgcagt aaacccgata gcgcgcttga cgcgccgcag
     3361 cttccaaacc gatttcacga gcgccggaaa ctttcacttt ggccatccgc agagtaacac
     3421 cgcaagccct gcactggcgc tcgcaaaacc tcgcccagct cgaagcgttg gggcttaatt
     3481 ggtgattgac gtggacggcg gaaagtttga aacggagctt cttggagagc gcttgcagaa
     3541 tgtcgagcag caccatcgag tccactccgc cactcaagcc gagcgcaaga tgcgggttgt
     3601 tggactttag taccttggcc agaacccctg caacatgcga gcgcaggtca caacggggcg
     3661 atttctttga acttgccata gcccatcaac cgctcgaagc gcgcgcgcag caattcatcg
     3721 agggatttgt cctgcaattg cttgagcgtg tcctgcagcg cttttttcat gagctgcatc
     3781 atttcctcgt gattgcggtg cgctccgccc agcggctcat tgatgatttt gtcgacgagg
     3841 cctagggttt tcagacgcgt cgcggtaatc cccagcgttt ccgcggcgtc cggcgccttg
     3901 tcagcgcttt tccacagtat ggaggcgcaa ccttcagggg aaatcaccga atacgtcgcg
     3961 tattgcagca tcaggatcgt atcgcccacc gcaatcgcca gcgcgccgcc ggaaccgcct
     4021 tcgccaatca cggcgcagac aatcggcacc cgcagttccg acatcacgta aagattacgg
     4081 ccaatggctt ccgactgccc tctttcttcc gcgcccacgc cggggtaagc gccgggtgta
     4141 tcaataaagg tcagcaccgg aacgccgtat ttttcggcgg tcttaatgag ccgcagcgcc
     4201 ttgcggtaac cttcaggacg cggcatgccg aaattgcgac ggattttttc cttcgtatcg
     4261 cggcccttct gatggccgat cgccatcacc gtctggccgt tgaaacgcgc gagcccgccg
     4321 acaatcgccg catcatcggc gaagcgacgg tcgccgtgca gctcctcgaa gtcggtgaac
     4381 aggttctgaa cgtaatccag cgtgtagggg cgttgcggat gacgcgccac ctgtgaaatc
     4441 tgccaagcgg tgagtttgga ataaatttcc ttggtgagcg cctggctttt tttctgcagg
     4501 cggtttattt cttcggaaat atccagcgcc gagtcgtcct gaacatagcg cagctcttcg
     4561 attttcgcct caagttccgc tatcggctgc tcaaaatcca gaaacgtcgt tttcataaat
     4621 aacgggtgat tttggttggg ctaatgatac aggatttcag ccacggccga agcgggctac
     4681 cagcccagcc caccgccctg cccgcgcgga tcgctggcag cctcggcgcg ccctttaagt
     4741 ttgttgatat aaaccgcctg catattgccc cagcgccttc cgcttttgga aataacatgg
     4801 cctttggctt gcagctttcg aatccactcc tcggaaaatc ctcgcggttc gatttcaatc
     4861 cggtcaggca ggaattgatg atgatagcgc ggcagaccca gcatcgcgtc cagatcaaac
     4921 cgtggtgcgc ccgcataatc gagcagcgcc agcagcagca tgctgatgat gcgcgagccg
     4981 cccggcgtgc ccaaaatcag aatgccgcgc tcgtcctcga caaaagtcgg cgacatgctg
     5041 gatagcggtc gtttccccgg agcgatttca ttcccgtgaa aaccctgcag gcggtaggca
     5101 ttgggaacgc ccggagcaac cgaaaaatca tccatttcat tgttgagcaa aacgccggta
     5161 ttccccgcca cgaaaccgct gccgaacggc gtattgatgc tcaaggtcgc ggccacccgg
     5221 ttgccttgcc tatctacgat ggaaaaatgc gtggtatcgg ccccttcgcc gattgcatcc
     5281 ggtatttcgc ccaatttgac gctcggcgtg gcatgctcag gatcaatggt tacagcgcgc
     5341 gaagccgcat aatccttgct cagcagctgc tcgaccggga cgctcacaaa atccgcatcg
     5401 cccagatagc gcgcccgatc ctgataggca cggcgcaacg cttccgccac aaaatgggcc
     5461 tgggtttcag cggtcgccgt ctcgaaagga agcacgctca ggatgttcag gctctgcgcc
     5521 agggtgagcc cgcccgcgga aggcaaagat gctgcggtaa tcttcagcga acggtaagcg
     5581 aattgaatgg gccggcgttc tatcacccgg taattcttaa gatcatccaa ctcccaaatt
     5641 ccgccgccgg cctgcaccgc cgcaaccatc tcttgcgcga ccctgccttg gtaaaaaccg
     5701 tcgtcgcctt tgcttgcaat cgcttgcagg gttgcggcga gccctttttg ctttaacacg
     5761 aaacccgcct ggggcacgct accgtaatcc agaagcaaac gccggccttc cccggtaagc
     5821 tggctttgaa agcgcagcgc cagcgccgca tagcgcgcat cgaccgcaaa acccgaacgg
     5881 gcgtagccaa tggcggcagc gaggctcact ttcagcggca gcctaccgta atgcctggca
     5941 agatagtcca gcgcggcggg cgtgccggga atggccgctg ctatcggccc gcgcagcgcg
     6001 gccttggaaa tgacctcgcc ccgctcatcc agataaagcg aaggggcggc tttccccggc
     6061 gcggtttcac gcgcatcgag catcacctct ttgccgtcgt ttgctcgatg caaaagccag
     6121 aacccccctc cgcccagtcc ggaagaataa ggttcaacca ccgctagcac cgctgctacc
     6181 gccaccgcgg cgtcaaaggc gttgccgcct tttttgagaa tctggtaacc ggcttgagtg
     6241 gcgagaggat gagcgcaggc tattgccgcg ttggaaaaat gttcgggagg cgccgccgga
     6301 aaaaccgcaa gcggcaaaag caccgaccac agaacgatga accggcaccg gcgcatcaag
     6361 cccgggcttt caatgaactg cgccaatacc gaagaaccgt tttatttttt cgaggatctc
     6421 gccgcgctgc tgcgcgattt cggcggcgct tttctcggcg ctcatgtttt gccgtatgcg
     6481 ctccagggcc acgcggcgcg tagcgaggat ttgcgaaatt tccccggcaa tgtcgggacg
     6541 cgcgcgcagg atgtcttcaa acgcgctctt gtcgaggcgg tagcattcga catcggtttt
     6601 cgcgatcacg ctggcgcgcc gtggatcgcc ggtcatcaac cccatctcgc cgaagaaact
     6661 gccggcgggc agcgtatcca aaatcagctt ttcgccttca ggcgtatcca gcgccacttc
     6721 tgcttcgccc gaagtcatga tgtacagcca atgcgcaatc gcgccctgcc tggtcatcac
     6781 ctcgccccgg gcgaacggcg cgtacttcag ccgctgcgcc agggtacgca gctcttcatc
     6841 actaaaatct tcaaacaggt cgacattctt caacgcagca aagcggcgat tgagttcgcg
     6901 cgaccgcgcc atctgctcgc gctgctcgtc ctgtttgatc acacgcacgc tttgctcgga
     6961 aagcgcaaag cgcaagccaa cgcgctgcag ggcggcgtaa atatgcgcac gcaccgccga
     7021 gtcagtgacg tcgtcataga gcaaatcggt aagccagtaa cgtagcgcgt agcgtcccga
     7081 gccgctttca aattccatca gaatgcagct cgctggcggc tgtttcgcca cgtgcgcaat
     7141 gtccgcctgg ttgatggcca gccccaccac actaattacc tgggaaggcg cgacgccgat
     7201 gtcgatgctg aataaaatcg agcgccgcca ttgctccggc tggccgctct tgcgaccgag
     7261 cacggtgaat ttgtttttca tgagctggct gttgggaaag acaactgttt cccagttgcg
     7321 tgtctcgatg gtagtcgagc gccagcggat ttccatgact ttgcccacca gatcatccac
     7381 cttgatccag tcgccgggcc ggatggaatt gtcgagctgc aaggccagtc cgcccagcac
     7441 attgcccagc gtgtcttgca tggaaaaggc gactacgccg gttatcaccg ccgaagtggc
     7501 gatgatgccg ccgagatcca ggcccacgta acgcaattgc accagacccc atgccgcata
     7561 agcgatgatg gtgaggatgt cctcgatgat gcg
//
LOCUS       DATIOW010000074        17065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1249978, whole genome shotgun sequence.
ACCESSION   DATIOW010000074 DATIOW010000000
VERSION     DATIOW010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17065
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249978"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VLL03_02205"
     CDS             complement(<1..1075)
                     /locus_tag="VLL03_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSS46211.1"
                     /translation="MEPHIFFLHLGIILLAARFLAEFASRFSVPSVIGELAAGLIFGP
                     SLVGWLAPDETLKILAEVGIILLLFEVGMDTDVFRMAKAGAKPVMVAMAGFLLPLILG
                     FAAAYLLFDLSLLASLFIGGTLTATSIGITVRVLTDLKRRASDEAQIVIGAAVIDDIL
                     GVIALAFLYQFAVQGQVTMSSIGQVSLFIILFMLLAPVAAKIIGWVIDHYDKKSEHPG
                     LLLTMVVSLIMMFSYLAHAVGAPAIMGGFAAGLAMGEQFRVKLSRSLPVPFRRQLNQV
                     LAASPQLTERLERQMRPLVHLFSPIFFVMVGVSLDLNTVNWNSALVWALSLALVVIAI
                     AGKWLAGFCVDENRLRQSAIGLSM"
     gene            complement(1095..2138)
                     /gene="mutY"
                     /locus_tag="VLL03_02210"
     CDS             complement(1095..2138)
                     /gene="mutY"
                     /locus_tag="VLL03_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HSS46212.1"
                     /translation="MSDFADHIISWQKRHGRHNLPWQNTHNPYRIWLSEIMLQQTQVT
                     TVIPYYRRFVKRFPNIKSLASAELNEVLTLWSGLGYYSRARNLHRATQIIARGHAGKF
                     PRDFESVLNLPGVGRSTAAAICVFAFGQRHPILDGNVKRVLARYFGIKGYPGEKKVEA
                     KLWRQSELLLPKRNIETYTQALMDLGATVCLRRKPLCAQCPLSTACRAFNKRMTSELP
                     APRPKKTLPRKQTVMLILLHKGEILLEKRPPAGIWASLWGFPEMAPEDDVRKVCSQRF
                     GARIRKPCFLPRLEQGFSHFELSIQPLLLQVEALNFHAAEPGQLWLNLNDAQGAAVPA
                     PVRKLLRQVAVYG"
     gene            complement(2236..4479)
                     /locus_tag="VLL03_02215"
     CDS             complement(2236..4479)
                     /locus_tag="VLL03_02215"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HSS46213.1"
                     /translation="MKKIIRYALLSIAGLIVVLAGLAAYIAATFDPNQYQPQIMQLVK
                     DKTQRTLKIDGDIKLSFFPSIGVNLPKLALSEHQSHREFAAVDSARVSLKLMPLLSKQ
                     VVVDEVEVKGLRANLTRHKDGHTNIDNLIGAGEAKPGAMAEAKPGASQFDIGHVDIEN
                     STLVYTDEGSGAKYTLRDVHLKTGRIANGTPGQIEAAFNVSGNQPKFDLNTRLKTRLT
                     FDLAGQNYLLEAFDLALKGEAAGITKLALNAAGDVGAKLQSKEFSVDKLKFSMTGVKD
                     KDNLDVSLDAPKINLAQEKFSGDKVVLLAKVSGQNNATVHLTVSALQGTQQAFKTGAI
                     NLELDSKQGGQTLKAKLSTPLSGNLKDKQFNLPAIAANFTVSGPGLPNKSVSGELKGS
                     AAIDAAKEHVQASLSGRLEDSSLKAKLGIAGFARPAINFDVDIDKLDIDHYLPQKTAE
                     AVPAAKPAETNQPEKPIDLSALKNLNANGSLRVGNLKVANVKAAQVRVDLKAANGKVD
                     INPLAANLYQGSVNGSIGINAAATPAFTVKQNLKDISIGPLLKDATNYDALEGKGSVS
                     LDVVTQGATVSALKKALNGKAALNLADGAVKGINIGETIRNFKSKLGALKGQQTQAAD
                     KSQKTDFTELKASFNIRNGVAHNDDLSMKSPLLRLSGNGDINIGEDSLNYLAKASIVE
                     SGKGQGGADISSLKGVTIPVRVSGPFKAPQYSLDFGGVATEIVKQKAEEAIKGKLQDK
                     LKGLLGK"
     gene            4668..4961
                     /locus_tag="VLL03_02220"
     CDS             4668..4961
                     /locus_tag="VLL03_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46214.1"
                     /translation="MMTRDEYVKKLKTQLDQWNSEIAKWEEKTRSAQTKMKADYEKQL
                     KAFRSRRDEAMQQLRQVQSASGEAWTEMVRGADEAWARLRDAFTKARSHFEKK"
     gene            complement(5264..5337)
                     /locus_tag="VLL03_02225"
     tRNA            complement(5264..5337)
                     /locus_tag="VLL03_02225"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5303..5305),aa:Gly,seq:ccc)
     gene            5383..5583
                     /gene="thiS"
                     /locus_tag="VLL03_02230"
     CDS             5383..5583
                     /gene="thiS"
                     /locus_tag="VLL03_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HSS46215.1"
                     /translation="MALTINGEPRHFDERLNVAQLLEKLELKGRRLALEKNGEIVPHS
                     RFNDEILRDGDQLEIVVAVGGG"
     gene            5738..6532
                     /locus_tag="VLL03_02235"
     CDS             5738..6532
                     /locus_tag="VLL03_02235"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HSS46216.1"
                     /translation="MDSLVIAGKTYTSRLLVGTGKYKDFAQTQAAIEASGAQIVTIAI
                     RRTNIGQNRGEPSLLDFIPPSTYTILPNTAGCYTAEDAIRTLRLARELLDGHDLVKLE
                     VLGDSHTLYPDIVETLGAAEKLVKEGFKVMVYTSDDPIVAKQLEEIGCVAVMPLASLI
                     GSGMGILNPHNLQIIIANAKVPVLVDAGVGTASDAAIAMELGCDGVLMNTAIAAAQNP
                     VLMASAMKKAVAAGREAYLAGRMPKKIYAASPSSPENGIIGHNKAA"
     gene            6550..7218
                     /gene="trmB"
                     /locus_tag="VLL03_02240"
     CDS             6550..7218
                     /gene="trmB"
                     /locus_tag="VLL03_02240"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007735204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HSS46217.1"
                     /translation="MKPIRSYVLRQGRISNAQKRAHETLLPKYGIPYAASLLDLNRAF
                     GRIAPKILEIGFGMGESTATIAREHPENDYLAIEVHTPGVGNLLKQIDAAELSNVRII
                     QHDAMEVLQQMLAPDSFSGVHIFFPDPWPKKRHYKRRLIQPDFVSLLCQRMKTGAYLH
                     VATDWQDYAEHILAVLDAELGLANTAENYASRPAYRPLTKFELRGLKLGHRVWDLVFK
                     RKPF"
     gene            complement(7237..8280)
                     /locus_tag="VLL03_02245"
     CDS             complement(7237..8280)
                     /locus_tag="VLL03_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HSS46218.1"
                     /translation="MKINRNQLQAAADQGWITAEQAERLWVFLGELHRDTPSFNFTHI
                     LYYLGGLIAIGAMTLFMTLGWEKFGGWGIFFISLAYAGAGLWLANYFIEHRQLPIPGA
                     IMATFVVALTPLAVYGLQNALGLWGGPHPYRDYHYYIDWNWIMMELTTLAAGAVMLWR
                     YRMAFLVMPVAVTLWYMSMDLAPFLFGGNSALDWDARRWVSVWFGFTIILFAFLVDLR
                     SRHSDKDYAFWLYLFGVMAFWGGLSLLDSKSELSKFLYCCLNLLMIVVSAVLSRRVFA
                     IFGGLGVAGYLGHLSYRVFKDSLFFPVTLTAIGLGVIWLGVLWQRHEARLTSGLHALL
                     PMGIRELLTRRQQ"
     gene            complement(8320..9504)
                     /gene="hemW"
                     /locus_tag="VLL03_02250"
     CDS             complement(8320..9504)
                     /gene="hemW"
                     /locus_tag="VLL03_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HSS46219.1"
                     /translation="MHVSLLTLYSSPCLSVLPPLSLYIHIPWCVKKCPYCDFNSHESR
                     SEIPEKQYVEALVADLESALSQISGRKIHSVFFGGGTPSVMSAETIDAILSAVRARLT
                     LDACAEITLEANPGTFEVAKFAAFRAAGVNRLSIGIQSFNPGHLKALGRIHDDLEARR
                     AVEIAQTHFDNINLDLMYALPGQKPGEALQDIKAAISYSPQHISAYHLTIEPNTLFHR
                     YPPRLPDDELSAQMQAAIEEALADNGYGHYETSAFAREGFQSRHNLNYWRFGDYLGIG
                     AGAHSKISTPQTIIRQMRYKQPQQYISRAIDGKSVQSQNIITTSDLVFEFMMNALRLI
                     EGFPAGLFEERTGLPITAVQRELDEAERRGLIVCDHRNIAPTLRGQRFLNDLLQIFLP
                     GE"
     gene            complement(9559..10146)
                     /gene="rdgB"
                     /locus_tag="VLL03_02255"
     CDS             complement(9559..10146)
                     /gene="rdgB"
                     /locus_tag="VLL03_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HSS46220.1"
                     /translation="MKKLVIASNNAGKLREIRELLASLKIEVLAQSAFDIPEVDEPHA
                     TFIENALAKARSASKLSGLPALADDSGICVDVLNGRPGVLSARYAGDPKSDERNNLKL
                     LQVLHAATDRRAHYYCVMALTRDADDPQPLIAEGAWHGEILHQPRGSGGFGYDPLFFV
                     PELGKSAAELPLEEKNRISHRSQALVQLIAKLREE"
     gene            complement(10264..10983)
                     /gene="rph"
                     /locus_tag="VLL03_02260"
     CDS             complement(10264..10983)
                     /gene="rph"
                     /locus_tag="VLL03_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HSS46221.1"
                     /translation="MRPSQRKTNELREVKITRHYTKHAEGSVFIECGDTKVICTASVD
                     EKVPLFLKGRGRGWVTAEYGMLPRSTGIRADREAARGRQSGRTQEIQRLIGRALRAVM
                     DPQKLGERTIQIDCDVIQADGGTRTAGITGGFVALHDAVTHLLKRKLITESPLNDFVA
                     AVSVGIYQGKPVLDLDYEEDSNCDTDMNVVMTGNSGFVEVQGTAEGEPFNREEMNALL
                     DLAQTGIKQLVAKQKAALKVK"
     gene            complement(10986..11897)
                     /locus_tag="VLL03_02265"
     CDS             complement(10986..11897)
                     /locus_tag="VLL03_02265"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HSS46222.1"
                     /translation="MKFTIYQKSRIGGRKHNQDRIAYSYSRDALLIVLADGMGGHLHG
                     EVAAQLAVQLIAESFEKHADPKLEEPRDFLRHAFHNAHDAILSYANDHDLLDSPRTTC
                     VACIVQDDLAYWAHVGDSRLYLFRGGKLLMRTRDHSKVQQLLDQGSLTPGQIAAYPER
                     NKIYNCMGGFLPPEVELSKKTAIRRGDTLMLCSDGLWGPLSDKEIANTISGYPVIQAV
                     QQLMDQAELREGGKSDNISAIGMTWGGDARDETTPEVSTVTMPLSAFTTQISSFNREH
                     AEAPDVTDEEIEQAIAEIQKTIEKYKK"
     gene            complement(11894..12823)
                     /locus_tag="VLL03_02270"
     CDS             complement(11894..12823)
                     /locus_tag="VLL03_02270"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HSS46223.1"
                     /translation="MPLPNGYQLLIYRVDKPLSRGGFSLVYRAFDEHNRYVAIKEYLP
                     SVLALRRDGYAVHATSADNLATFHYGMRCFFEEGRALARIIHPNVVRVLNFFRANDTV
                     YMVMQYERGRTLQEQIQQHKHTLSENFIRHMFSHLLNGLREVHTHKLLHLDIKPSNIY
                     LRATDGSPVLLDFGAARQTLTLDSPRLKPMYTPGFAPPEQYQSLDLLGPWSDIYSVGA
                     SIFSCLAASAPQPADLRVQNDKYETAKKTFGGKYSDELLDIIDWCLRLDPQERPPSVF
                     ALQKALLKKPGKAQKNNFLMSLRSKFGIGGADK"
     gene            12924..13790
                     /locus_tag="VLL03_02275"
     CDS             12924..13790
                     /locus_tag="VLL03_02275"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HSS46224.1"
                     /translation="MIYSMTGFAVATRELTRGALNLELRSVNHRYLDVQLRLPEELRL
                     LEPALREVIVSSVTRGKVDCRLSLEPAAGTPLAPQLNTELLQQLVEMNLVVRVALPDA
                     RSLSVADILRWPGLLGSDTLSPKDLRESCVSLLQKALDELNATRRREGEKLKGLLLER
                     IEKMERLVAEVAPRIPEIIAAYREKLGSRLSEALINREDDRIRQEFALFAAKIDVDEE
                     LTRLSTHLGETRQILEHGGAVGKRLDFLMQELNREANTLGSKSVDSRMSKVAMELKVL
                     IEQMREQIQNIE"
     gene            13896..14504
                     /gene="gmk"
                     /locus_tag="VLL03_02280"
     CDS             13896..14504
                     /gene="gmk"
                     /locus_tag="VLL03_02280"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HSS46225.1"
                     /translation="MTGNLFIVTAASGAGKTSLVRALLDYDPRVRKSVSCTTRAPRAG
                     EVNGQHYNFVSQQEFSEMLKRGEFLEHAEVHGNFYGTSKNWVEDAMKGGGDLLLEIDW
                     QGAQQVRRIFPQAVRIFILPPSLAVLEERLRTRGQDSMDAIASRLKAAREEMSHVQEF
                     DYVIINEEFRQAVHELICIVRAQRLKLKAQLERNHDLINRLK"
     gene            14518..14724
                     /gene="rpoZ"
                     /locus_tag="VLL03_02285"
     CDS             14518..14724
                     /gene="rpoZ"
                     /locus_tag="VLL03_02285"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HSS46226.1"
                     /translation="MARITVDDCMKKIPNRFELTLAATYRARQLATGASPLIEPNKDK
                     PTVIALREVAQGLVGVDILHKTQT"
     gene            14822..16939
                     /locus_tag="VLL03_02290"
     CDS             14822..16939
                     /locus_tag="VLL03_02290"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HSS46227.1"
                     /translation="MSDAEILFKELSGYLKSEDVAQLESAYHFSESAHQGQFRKSGDP
                     YISHPIAVANILAQWHLDPQALTAALLHDVMEDAHVTKDEISSRFGKPVAELVDGLSK
                     LDKIEFQTQEEAQAENFRKMLLAMARDVRVILIKLADRLHNMRTLEVMLPEQRRRIAR
                     ETMEIYAPIANRLGLNSIYQELQELSFRHLNPMRYQVLAKAVKAARGNRREVVGKILE
                     SIKQRLQEAGIEAQVTGREKHLYSIYKKMIEKSLSFSEVLDIYGFRILVKDASASYLA
                     LGALHSLFKPIPGKFKDYIAIPKANGYQSLHSMLFGPFGTPIEVQIRTADMHKIAEAG
                     VASHWLYKSSETSLNELQQKTHQWLQNLLEIQSESGDSVEFLEHLKVDLFPDEVYVFT
                     PKGQIMALPRGATAVDFAYSVHTDIGNRCVAVKIDHELMPLRTELKSGDRVEIITASH
                     AKPNPSWLNFVVTGKARSHIRHFLKTMHYEESVRLGERLLNQALSAFKPGKVQIDDAQ
                     WGKLLRDSSAKSRDEILADIGLGKRLGIVIARRLLSIRAPLTSEGRKIPGTVTIRGTE
                     GMAVQFAKCCRPIPGDPIIGLIKKGQGLVIHTHDCPVMAKIHAAPEKMLDVEWDAQTT
                     KPFDVSIKLTVANQRGVLAKVAAEIAEAGSNIDNVSMEEGESSAYTTIHFTLQVTNRM
                     HLAQLMRGLRRIPEVVRIARVKG"
BASE COUNT         3816 a         4939 c         4773 g         3537 t
ORIGIN      
        1 tcatcgaaag gccgatggcc gactgacgca gacggttttc gtcgacgcaa aaaccggcga
       61 gccatttgcc ggctatcgca atcaccacca acgccagcga caaagcccac accaaagcgg
      121 aattccagtt cactgtgttg aggtcgagcg agacgccgac catgacgaag aaaatcggcg
      181 aaaacagatg caccagcggt cgcatctggc gctccaggcg ctcggtcaat tgggggctgg
      241 cggccagcac ctggttgagc tgccgccgga acggcaccgg caggctcctg ctcaacttga
      301 cgcggaactg ctcgcccatg gccaggcccg cggcgaaccc gcccatgatg gcaggcgcgc
      361 ccaccgcgtg ggcgaggtag ctgaacatca tgatcaagct caccaccatg gtgagcaaaa
      421 gcccgggatg ctcacttttc ttgtcgtaat gatcaattac ccagccgatg attttcgccg
      481 caaccggcgc aagcagcatg aacaggatga taaaaagact tacctgcccg atagaactca
      541 tggtcacttg accttgcacc gcgaactgat agagaaacgc cagagcgatc accccaagaa
      601 tatcgtcgat caccgccgca ccgatcacga tttgcgcttc gtcggaagcg cgccgtttga
      661 gatcggtgag cacccgtacc gtgatgccga tgctggtggc ggtgagcgtg ccgccgatga
      721 aaaggctggc aaggagcgat agatcaaaca gcaaataagc cgcggcaaaa cccagaatca
      781 gcggcaggag aaatccggcc atcgccacca taaccggctt cgcgcccgcc ttcgccatgc
      841 gaaacacgtc ggtatccatg ccgacttcaa ataaaagcaa aatgatgccg acttcagcaa
      901 ggattttcag cgtctcgtcg ggcgcaagcc agccgacgag cgaaggaccg aaaatcagcc
      961 cggcggccag ttcgccaatc accgagggca cgctgaagcg gctggcgaac tcggcgagaa
     1021 aacgcgctgc caacaggatg atgcccagat gcagaaaaaa gatatgcggc tccattcacc
     1081 ccccagctac gctgttaccc gtaaaccgcg acttgccgca atagcttacg caccggcgcg
     1141 ggcactgccg cgccttgcgc atcgttaaga ttaagccaca actgcccggg ctccgccgcg
     1201 tggaaattta aagcctcgac ttgcagcaaa agcggctgga tactcaactc aaaatggctg
     1261 aagccttgtt ccaggcgcgg caaaaaacac ggtttcctta tccgcgcgcc gaagcgctgg
     1321 ctgcaaactt tgcgcacatc gtcttcaggc gccatttccg gaaaacccca cagactggcc
     1381 cagatgccgg caggcgggcg cttttccagc aatatctcac ctttgtgcaa taaaatcagc
     1441 atcacggttt gcttgcgcgg caaagtcttt ttcggcctgg gcgcgggtaa ctcgcttgtc
     1501 atgcgcttgt tgaacgccct gcaggctgtg ctgagaggac attgcgcgca aagcggcttg
     1561 cgccgcaggc agaccgtagc gccgagatcc atcagggctt gcgtgtaggt ctcgatattg
     1621 cgcttcggca gcagcagctc ggattggcgc cacagtttcg cttcgacctt tttttctccc
     1681 ggataaccct tgatcccgaa atagcgcgcc aatacccgct tcacattgcc gtccagtatt
     1741 gggtggcgct gtccgaatgc aaatacgcaa atcgccgcgg cggtcgagcg gccgacgcca
     1801 ggcaggttca acacactctc gaaatcgcgt ggaaattttc cagcgtgccc gcgcgcgatg
     1861 atttgcgtgg cgcggtggag atttcgcgcc cgcgagtaat accccagccc gctccagaga
     1921 gtcagcacct cattcagttc cgcgctagcc aggcttttga tgttgggaaa acgcttaaca
     1981 aaacgccggt agtaggggat gaccgtcgtc acctgcgtct gctgcaacat gatttccgac
     2041 aaccaaatgc ggtaggggtt gtgcgtgttc tgccacggca ggttatgccg gccgtggcgt
     2101 ttttgccagg atatgatgtg atccgcgaaa tcagacatga tgaaatgtta accgcagagt
     2161 tgcagagacg cggagacaga gcaaatcaac tgaagtcctt ctctgcgcct cagcgtctcc
     2221 gcggttgaag ctcagctatt tacccaacaa gcccttgagc ttgtcctgca gtttgccttt
     2281 gatcgcctct tcggcttttt gcttgacgat ctcagtcgcc acgccgccga aatccaggct
     2341 gtactgtggc gccttgaacg ggcccgacac ccgcaccggt atggtgactc ctttaagcga
     2401 ggaaatatcg gcgccgccct gtcccttgcc gctctccacg atggacgcct tggcgagata
     2461 gttcaggcta tcttcgccga tattgatgtc gccattcccg ctcaagcgca acagcggcga
     2521 tttcattgaa agatcgtcgt tgtgggcgac cccgttcctg atgttgaagc tcgctttgag
     2581 ttcggtgaag tcggtcttct gcgacttgtc cgcggcctgg gtttgttgac ccttgagcgc
     2641 gccgagcttg gatttgaagt tgcgtatcgt ttcgccaatg ttgatgcctt tcaccgcgcc
     2701 gtcggcgaga ttgagcgccg ccttgccgtt cagcgctttc ttcagcgcgc tcacggtggc
     2761 gccttgcgtg acgacatcca gcgacacgct gcccttacct tcgagcgcat cgtaattcgt
     2821 cgcatccttg agcaaggggc cgatgctgat gtccttgagg ttctgtttca ccgtgaacgc
     2881 aggggtggcc gcggcattga tgccgatgct gccgttaacc gagccctgat acagattggc
     2941 ggcaagcgga ttaatatcga ccttgccgtt cgccgccttg aggtctacgc ggacttgcgc
     3001 cgccttgacg ttggctactt tgagattgcc cacccgcagg ctgccgttgg cgttgaggtt
     3061 tttcagcgcg gagagatcaa tcggtttctc gggttgattg gtttcagccg gtttggccgc
     3121 gggtacggct tcagcggttt tctgcggcag ataatggtca atatcgagtt tgtcaatgtc
     3181 cacatcaaaa ttgatggcgg gcctggcaaa accggcgata cccagcttgg ctttcaagga
     3241 gctgtcttcc agtctgccgg aaagactagc ctgcacatgc tctttcgccg catcgatcgc
     3301 ggcgctgccc ttgagctcgc cggaaacgct cttgttggga agaccgggac cgctgacagt
     3361 gaaattggcg gcaatcgcgg gaaggttgaa ctgcttgtcc ttaagattgc cggagagcgg
     3421 cgtggaaagt ttcgctttca gcgtttgccc gccctgtttg gagtcgagtt ccagattaat
     3481 cgcgccggtc ttgaacgcct gctgtgtgcc ctgcagcgcc gacacggtca gatggacagt
     3541 ggcgttgttc tggccgctca ctttagccag caagaccact ttgtcgccgg aaaatttttc
     3601 ctgcgccagg ttgattttcg gggcatcgag gctgacatcc agattgtcct tgtccttgac
     3661 gccggtcatg ctgaatttga gcttgtcgac gctgaattcc ttgctttgca atttggcgcc
     3721 aacatcccct gcggcgttaa gcgcaagttt ggtgatgccc gccgcttctc ccttgagcgc
     3781 cagatcaaac gcttccagca aatagttttg gcctgcaaga tcaaatgtca gccgcgtctt
     3841 gagccgggta ttcagatcga acttgggctg gttgcccgag acgttgaaag ctgcttcgat
     3901 ctgccccggc gtgccgttgg cgatgcgccc cgttttcaaa tgaacatcgc gcagggtgta
     3961 tttggcgccg gaaccttcgt cggtgtaaac cagcgtggag ttttcgatat ccacatggcc
     4021 gatgtcgaac tgggacgcgc ccggtttcgc ttcggccatg gccccgggct tcgcttcgcc
     4081 cgcgccgatc aaattgtcga tattggtgtg gccgtctttg tggcgcgtaa ggtttgcgcg
     4141 caggcctttg acttccactt catccaccac cacctgcttg ctcaggagcg gcatgagctt
     4201 cagtgatacg cgcgcgctat ccacggcggc aaactcccgg tgggactggt gctcgctcaa
     4261 ggccagcttg ggcagattga caccgatgct gggaaagaaa gaaagcttga tatcgccgtc
     4321 gattttcagc gtgcgctgtg tcttgtcctt caccagttgc atgatttgcg gctggtactg
     4381 gttgggatca aaagtcgcgg cgatataagc cgcgagtccc gccagcacca caattaggcc
     4441 ggcgatggac agcaatgcgt atcggatgat ttttttcatg gtctatagcg gattgggtga
     4501 aatggaggga acagcccggt caattgttgt tcgccctaga ttataacagc ctgacataat
     4561 ctgcacttta ccgcttcgac aggccacctt tcctggcttt cagcgagtgg tataatttaa
     4621 ttgtaagttg caagttccta tccctcccca ttcagcagga gaacgccatg atgacccgtg
     4681 acgaatacgt aaagaaactg aagacacagc tcgaccaatg gaacagcgaa atcgccaagt
     4741 gggaagaaaa gaccaggtct gcccagacca agatgaaggc cgattatgag aaacagctca
     4801 aggctttccg ctcgcgccgc gacgaggcga tgcaacagtt acgacaggtt cagtcggcct
     4861 cgggagaggc gtggacggaa atggtgcgcg gtgccgacga ggcgtgggca aggttgcgcg
     4921 acgcgttcac caaagcgcgc tcgcatttcg aaaagaaatg accgggaaat aagcaaagcg
     4981 cttcctgtcg tcggccgcgg ggtctgtttc acggacctcg ggggaggtgg tatttccgca
     5041 tgcaccatcg cgccggaaga cttcctgaac ctcgcgatca acgttcccca atgcgggaaa
     5101 ccggcatccc atagttcacg actgcaaaag cttccggccc tatcgcactc catccctgaa
     5161 cctgacaaca aagtctaccg gcatctaacc ccgtgaggat cggggaattt tctggatcag
     5221 cccactccca aagactaggc ggtgcactgt acgaaaaagg tgatggagcg ggtgatggga
     5281 atcgaaccca cgtctaaagc ttgggaagct ttcgttctac cattgaacta cacccgcgtg
     5341 atagaattcc attgtatcca attcatggca aatgacaaaa tgatagcgct gaccattaac
     5401 ggcgagcctc gccattttga cgagcggctc aacgtcgccc aactgctgga aaagctggag
     5461 cttaagggca ggcggctggc gctggaaaaa aacggcgaaa tcgtgccgca cagccgtttc
     5521 aacgatgaaa ttctccgtga cggagatcag ctggaaatcg tggtggccgt cggcggcggg
     5581 taaagcctta aaccgcagag gacacagagg aacagcacaa ctcatcgtta acagcgatga
     5641 tctcgtcgaa aaaactactt gattttcaaa tgacacttcc tttcaaaaaa ataagggttt
     5701 catccgcgtc ctccgcggta aaatattcag ttaaactatg gacagtttag ttatcgccgg
     5761 taaaacctat acttcgcgcc tgctggtcgg cactggcaaa tacaaggatt tcgcgcagac
     5821 ccaggccgcg atcgaggcaa gcggcgcgca aatcgtcacc atcgccatcc gccgcaccaa
     5881 tatcggccag aaccgcggcg agcctagcct gctggacttc atcccgccct caacctacac
     5941 gattctcccc aacaccgcgg gctgctacac ggcggaagat gcgatccgca cactgcgcct
     6001 ggcgcgcgag cttttggacg gacatgatct ggtgaagctc gaggtgctgg gcgactcgca
     6061 tacgctttat cccgacattg tggaaacctt gggcgcggcg gagaaactgg tcaaggaagg
     6121 cttcaaggtg atggtctata cctcggatga ccccatcgtg gcgaagcagt tggaggaaat
     6181 cggctgtgtc gcggtgatgc cgctggcatc gctgattggg tcgggaatgg gcattctcaa
     6241 tccgcacaac ctgcaaatca ttatcgccaa cgccaaagtg ccggtgctgg tggatgccgg
     6301 cgtgggcacc gcttccgatg cggcgattgc gatggagctc ggctgcgacg gcgtgttgat
     6361 gaacaccgcg atcgccgccg cgcaaaatcc agtgctgatg gcttccgcca tgaaaaaagc
     6421 cgtcgcagcg gggcgcgagg cgtatctcgc cggacgcatg ccgaaaaaaa tctatgccgc
     6481 ctcgcccagt tctcccgaga acggcatcat cggccacaac aaagccgcgt aaccgtcccg
     6541 cactttccga tgaaaccgat aaggagctac gtcctgcggc agggcaggat ttccaatgcc
     6601 cagaaacggg cgcacgaaac cctgctgccc aaatatggca ttccctatgc cgcatcactg
     6661 cttgatctca accgtgcgtt tggccgcatt gccccgaaga ttctggaaat cggcttcggc
     6721 atgggcgaat ccaccgcaac gattgcccgg gagcatcccg aaaacgatta cctcgctata
     6781 gaagtgcaca cgccgggcgt gggcaatctg ctcaagcaaa tcgatgccgc ggaattaagc
     6841 aatgtgcgga tcatccaaca cgacgctatg gaagtcttgc agcagatgct tgcgccggat
     6901 tcgttttccg gcgtgcatat ttttttcccc gatccctggc cgaaaaagcg ccactacaag
     6961 cggcgcctga tccaaccgga ttttgtttcg ctcctgtgcc aacgcatgaa aacaggagcc
     7021 tatttgcacg tagcgacgga ctggcaggat tatgccgagc atattctcgc ggtgctcgac
     7081 gccgaactgg ggctcgccaa tacggctgaa aattacgcct cgcgccccgc ataccgcccg
     7141 ctcaccaagt ttgagttgcg ggggttgaag ctggggcatc gggtatggga tttggtgttc
     7201 aagcgcaaac cgttctaaac cgccggttgg cgacgcctac tgctgccttc gggtaagcaa
     7261 ttcgcggatt cccatcggca gcagcgcatg caaaccagat gtcaagcgtg cttcatgccg
     7321 ctgccagagc acccccagcc atatcacgcc caggccaatg gcagtaagcg tcaccggaaa
     7381 gaacaggctg tccttgaaca ccctataaga aagatgaccc agatatcctg cgacgccgag
     7441 cccgccgaat atggcgaaca cgcgtcgcga cagcactgca ctcacgacaa tcatgagcag
     7501 attgaggcaa cagtacagaa acttgctcaa ttcgcttttg gaatccaaaa gtgacagccc
     7561 gccccagaac gccatcacgc cgaaaagata cagccaaaag gcataatctt tgtcactgtg
     7621 acgtgaacgc aaatccacga gaaaagcgaa caagatgata gtgaacccga accagacaga
     7681 aacccacctg cgggcatccc aatccagtgc cgaattgccg ccgaacagga agggcgccaa
     7741 gtccatgctc atgtaccaca aagttactgc gaccggcata accagaaacg ccatgcggta
     7801 gcgccacagc atcaccgccc ccgccgccag tgtagtgagt tccatcatga tccaattcca
     7861 gtcgatgtag tagtggtaat cgcgataagg atgcgggccg ccccaaagcc ccagtgcatt
     7921 ttgcaggccg taaacagcca gcggcgtgag tgccaccaca aaagtggcca tgatagcgcc
     7981 gggtattgga agttgtctgt gctcgatgaa ataattcgcg agccacaaac cggcaccggc
     8041 gtaagcgagc gaaatgaaga aaatccccca gccgccaaat ttttcccaac cgagcgtcat
     8101 gaacagagtc atggcgccaa tggcaatcag tccaccgagg taatagagaa tatgcgtgaa
     8161 gttgaaactt ggcgtatcgc ggtgcagctc gcccagaaac acccacagtc gctccgcctg
     8221 ctcggcagtg atccaccctt ggtcagcggc agcctgcaat tgattgcgat taattttcat
     8281 gtttcctcct ccctctgcgt ttcagacgca ccgtgccgtt cattcgccgg gtaaaaaaat
     8341 ctgcagcaaa tcgttgagaa agcgctgccc gcgcaaagtc ggcgcgatat tccggtgatc
     8401 gcaaacgatg agcccgcgcc gttccgcttc atccagttcc ctttgcacgg cggtgatcgg
     8461 caatccagtc cgctcttcaa acaagccggc gggaaaacct tcgatgagac gcagcgcatt
     8521 catcatgaat tcgaagacga gatcgctggt ggtaataata ttttggcttt gaacactttt
     8581 tccatcaatc gcccggctga tgtattgctg cggctgcttg tagcgcatct ggcggattat
     8641 tgtttgcggc gtgctgattt tgctgtgcgc gccagcgccg atgcccagat aatcgccaaa
     8701 ccgccagtaa ttgaggttat gcctggattg aaagccttcc cgcgcgaaag cggaagtttc
     8761 gtaatgcccg tagccattat ccgcaagagc ttcctctatc gctgcctgca tttgcgcgct
     8821 caactcatca tccggcaacc gcggcggata acgatggaac agcgtattcg gctctatggt
     8881 caggtggtag gcggaaatat gctgcggcga atatgaaatt gcggccttga tgtcctgaag
     8941 cgcttctccc ggcttctgcc ccggcagcgc gtacatcaaa tccagattga tattatcgaa
     9001 atgcgtttgc gcgatttcga cggcgcggcg cgcctcaaga tcatcatgga tgcgtcccag
     9061 cgctttgagg tgtccgggat tgaagctctg gatgccgatg gagagacggt tcacgccggc
     9121 ggcgcgaaaa gccgcgaact ttgccacctc aaacgtgccg ggattggctt ccagcgtgat
     9181 ttcggcgcag gcatcaaggg tcaatctcgc gcgcactgcg gagagaatcg cgtctatagt
     9241 ttccgcactc atgacgctcg gtgtgccgcc gccgaagaac acgctgtgga tctttcgccc
     9301 cgagatctgc gacaaagcac tctccaaatc ggcgaccagc gcttcgacat attgtttttc
     9361 gggaatctcg ctgcgcgact cgtgagagtt gaaatcgcag taggggcatt tttttacgca
     9421 ccagggaatg tgaatataaa gcgaaagcgg cggcagcacg cttaagcacg gtgaggagta
     9481 aagagtaagg agcgagacgt gaatcttttc gctcccggtg ttttgctccc cggcttctcg
     9541 ctccgctctt cccactcttc actcctcacg cagtttggcg atcagctgca ccagtgcctg
     9601 gctgcggtgg ctgattcggt ttttctcttc cagcggcaac tccgccgcgc ttttgcccaa
     9661 ctccggaaca aaaaacagcg gatcgtagcc gaaaccgccg cttcctctcg gttgatgcag
     9721 gatttcgccg tgccaggcac cttcggcaat cagcggctgc gggtcgtcgg catcacgcgt
     9781 aagcgccatc acgcagtagt aatgcgcacg gcggtcagtc gcggcatgca gcacttgaag
     9841 aagtttcaga ttgttgcgct cgtcggattt ggggtcgccc gcgtagcgcg ccgacagcac
     9901 gcccggccgg ccgttcagca catccacgca aatgcccgaa tcatcggcaa gcgccggaag
     9961 tccgctgagt ttgctcgcac tccgcgcttt ggccagagca ttttcgataa aagtcgcatg
    10021 cggctcgtcc acctcgggaa tgtcgaaagc cgactgcgcc agcacttcga ttttcagcga
    10081 cgcaagcaac tcgcggattt cacgcagctt gcccgcatta ttgctggcga tgacgagttt
    10141 tttcatggat taaaacctgt taaccgcagt ggacgccgag gacgcggaga aaaccaaaaa
    10201 cgagaaaata tgatattaaa atgacttgcc tttgcgtcct ccgcgtcctc cacggtataa
    10261 gccttactta accttcaatg ccgctttctg tttggcgacg agctgcttaa tgccggtttg
    10321 cgcaagatca agcaacgcat tcatctcctc gcgattgaac ggttcacctt ccgccgtgcc
    10381 ctggacttcc acgaaaccgc tgtttccggt catcaccaca ttcatgtcgg tatcacagtt
    10441 ggaatcctcc tcataatcga gatcgagcac cggtttgccc tgataaatcc cgacagaaac
    10501 cgccgcgaca aaatcattga gcggcgattc agtgatgagc ttgcgcttga gcaaatgcgt
    10561 cacggcgtca tgcagcgcga caaagccgcc ggtgatgccg gcggtgcgcg tgccgccgtc
    10621 ggcctgaatc acgtcgcagt cgatttgaat agtacgctcg cccagttttt gcgggtccat
    10681 caccgcgcgc agcgcccggc cgatgagccg ctggatttcc tgcgtacgcc ctgactgcct
    10741 accgcgcgcc gcctcgcggt cggcgcggat tccagtcgag cgcggcagca tgccgtattc
    10801 ggcggtgacc cagccccggc ccctgccttt gagaaacaac ggcacctttt catcaacgct
    10861 ggcggtgcaa atcaccttgg tatcgccgca ttcgatgaac accgagcctt cggcatgctt
    10921 ggtgtaatgc cgcgtaattt ttacttcacg cagttcattg gttttgcgtt ggctggggcg
    10981 catgactact ttttgtactt ctcgatggtt ttctggattt cggcgatcgc ctgttcgatt
    11041 tcctcgtcgg taacgtccgg tgcctccgca tgctcgcggt taaaactgct gatttgcgtg
    11101 gtaaaggcgc taagcggcat ggtgacagtc gaaacttcgg gcgtagtctc gtcgcgcgca
    11161 tccccacccc aagtcatgcc aatagcgctg atgttgtcgc ttttcccgcc ttcgcgcaac
    11221 tccgcctggt ccatgagttg ctgcacggcc tggataacgg gataaccgga aattgtgtta
    11281 gcgatttcct tgtcggaaag cggcccccac aaaccgtcgc tgcataacat gagggtatcc
    11341 cctcgtcgta tcgcagtctt tttcgacaat tccacttccg gcggcagaaa cccgcccatg
    11401 caattataaa tcttgttgcg ctctggatag gccgcaatct gtcccggcgt gaggctgccc
    11461 tggtccagca attgctgcac cttggagtgg tcgcgggtac gcatgaggag cttgcccccg
    11521 cggaataaat acaggcgcga atcgccgaca tgcgcccaat aagcgaggtc atcctggacg
    11581 atgcaggcga cacaagtggt gcgcggcgaa tccagcaaat catggtcatt ggcgtaactc
    11641 aggatcgcat cgtgcgcgtt gtgaaacgca tgacgcagga aatctcgcgg ctcctccagc
    11701 ttggggtcag catgcttttc gaagctctcg gcaatcagct gcaccgccag ctgcgccgcc
    11761 acttcgccat gcaaatgccc gcccatgccg tcggcgagca ctatcagcag cgcatctcgg
    11821 ctgtaggaat aagcgatgcg gtcctggttg tgcttgcggc cgccgatgcg gcttttctgg
    11881 taaatggtga atttcactta tcggccccgc caattccgaa tttgctgcgc aagctcatca
    11941 ggaaattatt tttctgggct ttgccgggtt tttttagcag ggctttttgc agggcgaaca
    12001 cgctgggcgg gcgctcctgt ggatcgagac gcagacacca gtcgatgata tccagcagtt
    12061 cgtcggaata tttgccgcca aaggttttct tcgctgtctc gtacttgtcg ttctgcaccc
    12121 gcaaatccgc cggctgcggc gcgctggcgg caaggcagga aaagatactc gcacccacgc
    12181 tgtagatgtc gctccaaggg ccgagcaaat ccaggctttg gtattgctcc ggcggagcga
    12241 atccgggcgt atacatcggc ttcaggcgcg ggctgtcgag ggtaagcgtc tgacgcgccg
    12301 cgccgaaatc taaaagcacc ggcgagccgt cggtggcgcg cagataaatg tttgacggct
    12361 tgatgtcgag atgcagaagc ttgtgcgtgt gcacttcacg caggccgttc agcaaatggc
    12421 tgaacatatg gcggatgaaa ttttcggaaa gagtatgctt gtgctgctgg atttgctcct
    12481 gcagcgtgcg gccgcgctcg tactgcatca ccatgtacac agtatcgttg gcacggaaga
    12541 aattcaacac tcgcaccacg ttgggatgaa tgatgcgcgc cagcgcacgg ccttcctcga
    12601 aaaaacagcg catgccgtag tggaaggtgg cgaggttgtc agcggatgtg gcgtgcacgg
    12661 cgtagccgtc ccggcgcagc gccagcacgc tcggcagata ttccttgatg gcgacgtatc
    12721 tgttgtgctc atcgaaagca cggtacacga ggctgaagcc gccgcggctc aaaggtttgt
    12781 cgacacggta gatcagcagc tggtagccgt tgggaagcgg catattggat tgtggagtca
    12841 tgcccagtaa aaatatgcta tcgttttgaa atgaccatca ttgtcggtat ttgcgcggtt
    12901 aatgtaaagc atcgggaaaa aacatgattt acagcatgac gggcttcgcc gtagcgaccc
    12961 gggaactcac acgcggcgcg ctcaatctcg aactgcgttc ggtcaaccac cgctatctgg
    13021 atgtgcaatt gcggctaccg gaagaattgc gcctgctcga accggcgctg cgcgaggtaa
    13081 tagtaagtag cgtcacccgc ggcaaggtgg attgccgctt gagcctggag ccggcagccg
    13141 gaacacccct cgcgccgcag ctcaataccg aacttttgca gcaactggtg gaaatgaacc
    13201 tggtcgtgag ggttgcgctg ccggatgcac ggagcttaag cgtcgccgac attctgcgct
    13261 ggcccggctt gctgggaagt gacaccttgt caccaaaaga cctgcgtgaa tcctgcgtca
    13321 gcctgctgca aaaagcactc gatgagctca acgccacccg gcggcgcgaa ggcgaaaaac
    13381 tcaaaggttt attgttggaa cgcatcgaaa aaatggagcg tctggtggcg gaagtggcgc
    13441 cgcgtattcc ggaaatcatc gccgcctacc gggaaaagct cggatccagg ctttccgaag
    13501 cgctgatcaa ccgcgaagac gatcgcatcc gccaggaatt cgcgttgttc gccgccaaaa
    13561 tcgacgtgga cgaggaactg acgcgtctta gcacacacct gggcgaaacc cggcaaatcc
    13621 tcgagcacgg cggcgcggtg ggcaagcgcc tcgatttcct gatgcaggaa ttgaaccgcg
    13681 aggccaatac gctcggctcc aaatccgtcg acagcagaat gtcaaaagtg gcaatggaac
    13741 tcaaggtgct gatcgagcag atgcgcgagc aaatccaaaa catcgagtga ggagcaaggt
    13801 gtgaagagta aagtgttttt tgcgcaggcc acgaaaagca aaatccgagt cttatttttt
    13861 actcctgacc cctcactcct tcccccttac ttgctatgac tggtaatctc tttatcgtca
    13921 cagccgcttc cggcgcaggc aaaaccagcc tggtgcgcgc gttgctggac tacgacccgc
    13981 gggtgcgcaa gtcggtttcc tgcaccacgc gtgcaccgcg tgcgggagaa gtcaacggcc
    14041 agcactacaa tttcgtttcg cagcaggaat tttcagagat gctgaaacgc ggcgaatttc
    14101 tggaacacgc cgaggtgcac ggcaatttct acggcacgtc gaaaaactgg gttgaggatg
    14161 caatgaaggg cggcggcgac cttctgctgg aaattgactg gcaaggcgcg caacaggtgc
    14221 ggcgcatttt cccgcaagcc gtcagaatct tcatcctgcc gccgtcgctc gccgtattgg
    14281 aggagcgatt gcgcacccgc ggccaggaca gcatggacgc gattgcgagc cgtctcaaag
    14341 ccgcacgcga ggaaatgagc cacgtccagg agtttgatta tgttattatt aacgaagagt
    14401 tcaggcaggc agtgcatgaa ctgatatgca tcgttcgcgc gcaaaggctg aaacttaaag
    14461 cccagcttga gcgcaaccac gatctgatta accgattgaa gtgaatcgcg aggaaatatg
    14521 gcacgcatta ccgttgatga ctgcatgaaa aaaatcccca accgctttga attgacgctg
    14581 gcggcgactt accgcgcacg tcagctcgcc accggcgcaa gcccgctgat cgagccgaat
    14641 aaggacaaac caacggtgat cgcgctgcgt gaagtcgcgc aaggcctggt tggcgtggac
    14701 atcctgcaca agacccaaac ataagcggcc gtcagcggcc agctttcagc gatcagcttt
    14761 gagccgcggc catgcagctc ctgctttcct gattgctgac agctgatagc caacagctaa
    14821 aatgtccgat gcggagattc tgttcaagga attatccggc tacctcaagt cggaggacgt
    14881 cgcgcagctc gaatccgcct atcacttcag cgaatccgcg caccagggac agttccgcaa
    14941 gagtggcgat ccctacatct cccaccctat cgccgtagcc aacatcctcg cacagtggca
    15001 tctcgacccg caggccctta ctgcggcgct gttgcacgac gtgatggagg acgcgcacgt
    15061 caccaaggac gaaatcagca gccgtttcgg taaaccggtg gcggagctgg tggacgggct
    15121 ctccaagctc gacaaaatcg aattccagac ccaggaggag gcgcaggcgg aaaacttccg
    15181 caagatgctg ctcgccatgg cgcgcgacgt gcgcgttatt ctcatcaagc ttgccgaccg
    15241 tctgcacaac atgcggacgc tggaggtcat gctgccggag cagcgccgcc gcatcgcccg
    15301 cgagaccatg gaaatttacg cgccgatcgc caaccggctg ggcctgaaca gcatctatca
    15361 ggaactgcaa gaactgagtt tccgccactt aaatccgatg cgctatcagg tgctcgccaa
    15421 ggcggtgaag gcggcgcgcg gcaaccggcg cgaggtggtg ggcaagatac tcgaatccat
    15481 caagcagcgg ctgcaggaag cgggaatcga ggcgcaggtc accggccgcg aaaagcacct
    15541 ctacagcatt tacaagaaaa tgatcgagaa gtcgctctcc ttctccgagg tgctggatat
    15601 ttatggcttc cgcattctgg tcaaagacgc ttccgccagc tatctggctt taggcgcact
    15661 gcatagcttg ttcaagccga ttccgggcaa attcaaggac tacatcgcca ttcccaaggc
    15721 caacggctac cagtcgctgc attccatgct gttcggcccg ttcggcacgc cgatcgaagt
    15781 gcagatccgt acggcggaca tgcacaagat cgccgaagcg ggtgtggcct cgcattggct
    15841 gtacaaaagc tccgagactt cgctcaacga gctgcagcag aaaacccacc agtggctgca
    15901 gaacctactc gaaatccagt cggaaagcgg cgattcggtc gagttcctcg agcacctcaa
    15961 ggtggacctg tttcccgatg aagtgtacgt gttcacgccc aagggccaga tcatggcgct
    16021 gcctcgcggc gccaccgcgg tggatttcgc ctacagcgtg cacaccgaca tcggcaaccg
    16081 ctgcgtggcg gtgaaaatcg accatgagct gatgccgctt cgcaccgaac ttaagagcgg
    16141 cgaccgggtg gaaatcatca ccgcctcgca cgccaaaccc aacccctcgt ggctcaattt
    16201 cgtggtcacc ggcaaggcgc gctcgcacat ccgccacttt ctcaagacta tgcactacga
    16261 ggaatcggtg cggctgggcg agcgattact caatcaggcg ctatcagcgt tcaagcccgg
    16321 caaagtgcaa atcgacgatg cacagtgggg caagctgctg cgcgacagca gcgccaaatc
    16381 cagagacgaa atcctcgccg atatcggact gggcaagcgc ctgggtatag tgatagcaag
    16441 gcggttgctg tcaattcgcg cgccattgac cagcgaaggc aggaaaatcc cgggcactgt
    16501 cactattcgc ggcaccgagg gcatggcggt gcaattcgcc aagtgctgcc ggccgatacc
    16561 gggcgatccc atcatcggcc ttatcaagaa ggggcaaggg ctggtgattc acactcacga
    16621 ctgcccggtg atggccaaga ttcacgccgc accggaaaaa atgctcgacg tggagtggga
    16681 tgctcaaacc acaaagccgt ttgacgtgag catcaaactc accgtcgcca atcagcgtgg
    16741 tgtgctggcc aaggtcgccg cggaaatcgc cgaagccggc tccaacatcg acaacgtcag
    16801 catggaagaa ggcgaaagca gcgcctatac caccatccac tttaccttgc aggtcactaa
    16861 ccgcatgcat ctcgcccaat tgatgagggg cttacgcaga attcccgaag tagtgagaat
    16921 cgcgcgggtc aagggctgat acactgcaag aaaccgaaac gcgctacacc agaataacct
    16981 atccatcagc caggtctacg gcatcgctgc gagtaaatgc taggcacatg gtctgcccgt
    17041 atggatgttg ctgatcaacg ggacc
//
LOCUS       DATIOW010000075         8008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1860529, whole genome shotgun sequence.
ACCESSION   DATIOW010000075 DATIOW010000000
VERSION     DATIOW010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8008
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860529"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1703
                     /gene="carB"
                     /locus_tag="VLL03_02295"
     CDS             <1..1703
                     /gene="carB"
                     /locus_tag="VLL03_02295"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HSS46228.1"
                     /translation="TETAVRQKRLRLGVRPVYKRVDTCAAEFATRTAYMYSTYEEECE
                     AQPTSKRKIMVLGGGPNRIGQGIEFDYCCVHAALALREDGFETIMVNCNPETVSTDYD
                     TSDRLYFEPLTLEDVLEIVHVEKPEGVIVQYGGQTPLKLARNLEANGALIIGTSPDMI
                     DCAEDRERFQKMLDKLKLKQPPNRSARTPQDAFRLAEEIGYPLVVRPSYVLGGRAMEI
                     VHEQGDLERYMREAVKVSNDSPVLLDRYLNDAIEVDVDAVCDGKRVLIGGIMEHIEQA
                     GVHSGDSACSLPPFSLSNALQDELRRQTTAMAHALKVVGLMNVQYAIQGDTVYVLEVN
                     PRASRTVPFVSKATGLPLAKIAARCMVGRTLEEQGVTQEVIPPYYSVKEAVFPFIKFP
                     GADSILGPEMKSTGEVMGVGATFAEAFVKSQIAAGEKLPSSGRVFISVRNADKPRTVE
                     IAQVLQNLGFSLVATRGTAATLAAAGIKVTPVNKVAEGRPHIVDMIKNGEIDLIINTV
                     EDRRAVHDSYSIRHAALQRRVTYYTTLAGARAACTGIQHMREMRPYELQGLHRGLAAR
                     "
     gene            1783..2259
                     /gene="greA"
                     /locus_tag="VLL03_02300"
     CDS             1783..2259
                     /gene="greA"
                     /locus_tag="VLL03_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HSS46229.1"
                     /translation="MNKIPLTVSGAEKLRSELQQLKTVQRPTVITAIAEARTHGDLSE
                     NAEYHAAKERQSFIEGRIVELEAKLSNAQIIDPAVLDADGCCVFGATVELEDIGGGGT
                     LTYQIVGDDEADIKLGKISISSPIARALIGKYPGDVAEVQAPGGVRQYEILDVKYI"
     gene            2270..2731
                     /locus_tag="VLL03_02305"
     CDS             2270..2731
                     /locus_tag="VLL03_02305"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HSS46230.1"
                     /translation="MSYLADSLHWIAVTLWVGALWAVGYIAAPVLFYFFDDRVLAGTL
                     AARMFTLVAQVGMGCGAYLLLFRIIRQGVYSLKQWFFWITLLMLLLTLIGHFGAQPVM
                     ESLKQQSLAKEIVESAFKDRFATWHGVGNVLYLIESALGLVLVLLQPRAAP"
     gene            complement(2737..3075)
                     /gene="yhbY"
                     /locus_tag="VLL03_02310"
     CDS             complement(2737..3075)
                     /gene="yhbY"
                     /locus_tag="VLL03_02310"
                     /inference="COORDINATES: protein motif:HMM:NF014087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HSS46231.1"
                     /translation="MPTLNSSEHRSLRAKAHALKPVVIIGESGLTPAVMREIDLSLNH
                     HELIKVKVAGEDRARRENALEEICTSLNAAPVQHIGKILVIYRKTPEAEQAKKPRALP
                     GKRRQEPRVQ"
     gene            3103..3729
                     /locus_tag="VLL03_02315"
     CDS             3103..3729
                     /locus_tag="VLL03_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HSS46232.1"
                     /translation="MKRSKTSSAWMREHVNDLYVKRAKQEGFRSRAAFKLLEIDSRDK
                     LLLPGLTVVELGAAPGGWTQVVAEKVGPRGRVIALDMLEIRPVAGVDFIQGDFREKVV
                     LQELRRRLQGKTADLVISDMAPNVSGVASSDQARVVELASLALEFAENVLQPQGKLLV
                     KFFQGEGFEQFVAAMRRSFKEVVSRKPKASRDRSSEIYLLGKGLRPKD"
     gene            3818..5686
                     /gene="ftsH"
                     /locus_tag="VLL03_02320"
     CDS             3818..5686
                     /gene="ftsH"
                     /locus_tag="VLL03_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HSS46233.1"
                     /translation="MAIWLVIALVLMTVFNQFSQRQAAQSPMEYSQFIDEVKQGHIAK
                     VTIEGRTLKGVKTDGKRFVTYSPSDPWLVSDLLKNGVIIEAKPDEEPSLLVNIFVSWF
                     PMLLLIGVWIFFMRQMQGGGRGGAFSFGKSRARMMDESNNQVTFADVAGCEEAKEEVS
                     ELVDFLRDPSKFQKLGGRIPRGVLMVGNPGTGKTLLARAIAGEAKVPFFSISGSDFVE
                     MFVGVGAARVRDMFEQAKKHAPCIVFIDEIDAVGRQRGAGLGGGNDEREQTLNQLLVE
                     MDGFETGVGVIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDIRGREQILLVHMRKVP
                     IAPDVRADILARGTPGFSGADLANLVNEAALFAARFNKRLVDMDDFERAKDKVIMGAE
                     RRSMVMPEHERRNTAYHESGHAVVAKLLPKTDPVHKVTIIPRGRALGVTMQLPQEDRY
                     SLDREHILQNISVLFGGRIAEEIFMGQMTTGASNDFERATEMARNMVTRWGMSDELGP
                     MVYGENEGEVFLGRSITTHKNVSETTMQKVDAEIRRIIDQQYALARKLIEENRDKVEA
                     MVKALLEWETIDSDQINDIMEGRPPRPPKPVQPPQPSQDTPPGGATATAQPAARET"
     gene            5782..6609
                     /gene="folP"
                     /locus_tag="VLL03_02325"
     CDS             5782..6609
                     /gene="folP"
                     /locus_tag="VLL03_02325"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HSS46234.1"
                     /translation="MFLQCGKFKLDLSRPLIMGIVNVTPDSFSDGGKLVSAPAAINHA
                     KQLVEEGADILDIGGESTRPGAEPVSLEEELRRVLPVLEGLKDVNVPLSVDTYKPEVM
                     RAAISAGASMINDVNALLAPGAMESVAASDMALCLMHKKGEPKTMQQNPQYEDVLTEV
                     ADFLKARVAAAEAAGVARERICIDPGIGFGKNLEHNLALLRNIGRLKSIGVPVLIGVS
                     RKSLLGKILGLEVYDRVQVNAVAAVLTVRQGAAIVRVHDVKATKDALAVYNAVGREA"
     gene            6629..7984
                     /gene="glmM"
                     /locus_tag="VLL03_02330"
     CDS             6629..7984
                     /gene="glmM"
                     /locus_tag="VLL03_02330"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HSS46235.1"
                     /translation="MARKYFGTDGVRGTVGEAPITPDFVMRLGYAAGKVLAGMSKSTA
                     ERAAVLIGKDTRISGYMLESSLEAGLSAAGVDVLLVGPMPTPAVAYLTRALRLSAGIV
                     ISASHNPYNDNGIKFFSGTGLKLADEIELAIETELEKPLNCNASAQLGKARRIDDAAG
                     RYIEFCKSTFPSDLDLRGLRLVVDTAHGATYHIASHVFHELGADVIAIGNQPDGLNIN
                     QECGAAYPGSLLEAVKEHRADLGIALDGDGDRLLMADSGGKTYDGDQLLFIIAKHRQQ
                     QGTLKGGVAGTLMTNLGMQRALEKLRVPFARAAVGDRYVLELLLEKGWQLGGENSGHI
                     ICLDKHTTGDGIISALQVLCALRAYNKGLAELTRDLALYPQVLVNVKVVKGFDFREQP
                     PVKAALNRAEADLGDEGRVLLRASGTEPVVRVMVEGQPQNKVKHWADTIAQAVREAAV
                     K"
BASE COUNT         1685 a         2236 c         2508 g         1579 t
ORIGIN      
        1 ccactgaaac tgcggtgcgg caaaaacgtt tgcggctggg cgtgcgtccg gtgtacaaac
       61 gggtggacac ctgcgccgcg gaattcgcca cgcgcaccgc ctacatgtat tccacttacg
      121 aagaggaatg cgaggcgcag cccaccagca agcgcaagat catggtgctg ggcggcgggc
      181 ccaaccgcat cgggcagggc atcgagttcg attattgctg cgtgcacgct gcgctggcgt
      241 tgcgcgaaga cgggttcgag accatcatgg tcaactgcaa ccctgaaacc gtttccaccg
      301 attacgatac ctccgaccgc ctgtattttg agccattgac gctggaagac gtgctcgaaa
      361 tcgtgcacgt ggaaaaaccg gaaggggtga tcgtgcaata cggcgggcaa acgccgctga
      421 aactggcgcg caatctcgaa gcgaacggcg cgctcatcat cggcacctct cccgacatga
      481 ttgactgcgc ggaggaccgc gagcgcttcc aaaaaatgct ggataagctc aagctcaagc
      541 agccgcccaa ccgctccgcg cgcactccgc aggacgcttt tcgcctggcc gaggaaatcg
      601 gctatccgct ggtggtgcgg ccgtcctatg tgctgggcgg gcgcgccatg gaaatcgtgc
      661 acgaacaagg cgatcttgag cgttacatgc gcgaagcggt gaaagtttcc aacgattcgc
      721 cggtattgct cgatcgttat ctcaacgacg ccatcgaagt ggacgtggat gcggtgtgcg
      781 atggcaagcg ggtgctgatc ggcggcatca tggaacacat agagcaggcg ggcgtgcatt
      841 ccggcgattc cgcatgttcc ctgccgccgt tcagtttgag taatgcattg caggacgaac
      901 tgcgccggca aacgacggcg atggcgcatg cgctcaaggt ggtggggctg atgaacgtgc
      961 aatacgcgat tcaaggcgac acggtttatg tgctggaagt caatcctcgc gcctcgcgca
     1021 ccgtgccctt tgtgtcgaaa gccaccggtt tgccgctggc taaaatcgcg gcgcgctgca
     1081 tggtcgggcg cacgctggaa gagcaaggcg tgacgcaaga agtgattccg ccgtattact
     1141 cggtgaagga agcggtgttc ccgttcatca aattcccggg cgcagacagt attctcgggc
     1201 cggaaatgaa atccaccggc gaagtgatgg gcgtgggcgc cacctttgcc gaggcgtttg
     1261 tgaaatcgca aatcgcggcg ggggagaaac tcccttcttc cgggagggtg ttcatcagcg
     1321 tgagaaacgc cgacaagccg cgcaccgtcg aaatcgcaca agtcctgcag aatctgggct
     1381 tcagcctggt ggcgacacgc ggcaccgccg ccacgctggc tgctgccggc atcaaggtca
     1441 cgccggtcaa caaggtcgca gaaggccggc cgcacatcgt ggacatgatc aagaacggtg
     1501 aaatcgacct gattatcaac accgtggaag acaggcgcgc ggtgcatgat tcgtattcga
     1561 tacgccacgc cgcgctacag cggcgggtga cgtattacac cacgctcgcc ggcgcgcgcg
     1621 cggcgtgcac cggcattcaa cacatgcggg aaatgcggcc gtatgaactg caggggctgc
     1681 accgcggcct ggcggcacgt tgagtaactg tttatttttc accggaatac cgctataaat
     1741 agacgataag ccaagcattc ctgaaatagg ggacaaatcg cggtgaacaa aattccatta
     1801 actgtgagcg gggccgaaaa gcttcgcagc gagttgcagc aactcaaaac ggtgcagcgg
     1861 ccgacggtga ttaccgccat cgccgaggcg cgcacccacg gcgacctttc cgaaaacgcc
     1921 gagtaccacg ccgccaagga aagacaaagc ttcatcgagg ggcgcatcgt cgagcttgag
     1981 gcgaaactct ccaacgccca gatcatcgat cccgctgtgc tggatgcgga cggatgttgc
     2041 gtgtttggcg ccaccgtcga actggaagac ataggcggcg ggggcaccct gacctaccag
     2101 atcgtcggcg acgacgaggc ggacatcaag ctcggcaaga tttccatcag ctcgccgatt
     2161 gcgcgcgctt taatcggcaa atatcccggc gacgtggcgg aagtacaggc gccgggcggc
     2221 gtgcgccagt atgaaatcct cgatgtgaag tatatttaag gaggctaccg tgagttatct
     2281 tgccgacagt ttgcattgga tagccgtaac cctttgggtc ggcgccttgt gggcggtggg
     2341 ttacatcgct gcacctgttc tcttttattt tttcgacgac cgcgtgctgg cggggacgct
     2401 cgctgcaaga atgttcacct tggtggccca agttggaatg ggctgcggcg cttatttgtt
     2461 gttgttccgc atcatccggc agggcgtgta ttcgctcaag caatggtttt tctggattac
     2521 gctgttgatg ctgctgctca cgctgatcgg gcacttcggc gcgcagccgg tcatggaaag
     2581 cctgaaacag cagtcgctgg cgaaggaaat cgtggaaagc gcgttcaagg accgctttgc
     2641 tacttggcac ggtgtgggca acgtgcttta tctcatagaa agcgcgctgg ggctggtgct
     2701 ggtgctgctg cagcccagag cagcgcccta gggaacttat tgtactcgcg gctcttggcg
     2761 tcttttgccg ggcaaggcgc gcggcttctt cgcttgctcg gcctccggtg ttttgcggta
     2821 aatgacgagt attttgccga tgtgctgcac cggggctgcg ttaaggctgg tgcaaatttc
     2881 ttccagcgca ttttcccgcc gcgcgcgatc ttctcctgca accttgacct tgatcaattc
     2941 gtggtggttg aggctcaggt cgatttcccg cattacggcg ggcgtcagac cgctttcgcc
     3001 gatgatcacc acgggcttga gtgcgtgcgc cttggcgcgc aatgagcggt gttcactgga
     3061 attaagtgtt ggcatgattt cctctacagg caagtctagc gaatgaagcg ctccaaaacc
     3121 agcagcgcct ggatgcgcga gcatgtcaat gacctttacg tgaagcgcgc caaacaggaa
     3181 ggtttccgtt cgcgcgccgc cttcaagctg ctggaaatcg attcgcgcga caagttgctc
     3241 ttgccgggcc tgaccgtggt ggaactcggc gcggcgcccg gcggctggac gcaggtggtg
     3301 gcggagaagg tgggcccgcg cggcagggtg attgcgctgg atatgctgga aatacggccg
     3361 gttgccggcg tggatttcat tcagggcgat tttcgcgaaa aggtggtctt gcaggaactg
     3421 cgccgcaggc tacagggaaa aaccgcggac cttgtaattt ccgacatggc acccaatgtt
     3481 agcggtgtgg caagcagcga ccaggcgcgc gtggtggagt tggcctccct ggcactggaa
     3541 tttgcagaga atgtgctgca accgcaaggg aaactgctgg tcaaattctt ccagggcgaa
     3601 ggatttgagc aatttgttgc cgcgatgcgc cgcagcttca aagaggtggt ttcgcgcaaa
     3661 cccaaggcct cgcgcgaccg atcgagtgaa atctaccttc tgggcaaagg tttgcggccc
     3721 aaagactgac actggacagg aaatagggat agaataaggc tagctaactt ccgtttttgg
     3781 gatttcagga gcgaatttga acaatttagt aaaaaatatg gcgatctggc tggtgatcgc
     3841 gctggtattg atgaccgtct ttaaccaatt cagccagcga caagcggcgc aatcgccgat
     3901 ggagtattcg caattcatcg acgaggtgaa acagggtcac atcgccaagg tcaccatcga
     3961 aggccgcacc ctgaaagggg tgaagactga cggcaagcgt ttcgtcacct attcgccttc
     4021 cgatccctgg ctggtgagcg atttgctcaa aaacggcgtg attatcgaag ccaagccgga
     4081 tgaggagcct tcactcctgg tgaacatctt cgtttcctgg ttcccgatgc tgctgcttat
     4141 cggcgtgtgg attttcttca tgcgccagat gcagggcggc gggcgcggcg gcgcattttc
     4201 cttcggcaag agccgagcgc gcatgatgga tgaaagcaat aatcaagtca cattcgccga
     4261 tgttgccggc tgcgaggaag ccaaggaaga agtctccgaa ctcgtcgatt tcctgcgcga
     4321 cccctccaag ttccagaagc tgggcgggcg cattccgagg ggcgtcttga tggtgggcaa
     4381 ccccggcacc ggcaagacct tgctcgcgcg cgcaatcgcg ggtgaagcca aggtgccttt
     4441 cttcagcatt tccggctccg acttcgtgga aatgttcgtc ggcgtgggcg cggcgcgcgt
     4501 gcgcgacatg ttcgagcagg cgaagaaaca cgcgccgtgc atcgtgttca tcgatgaaat
     4561 cgacgccgtg ggccgccagc gtggtgcggg attgggcggc ggcaatgacg aacgcgaaca
     4621 gacgctgaac cagctgctgg ttgagatgga cggattcgag accggtgtcg gtgtgatcgt
     4681 gattgcagcg accaaccggc ctgatgtgtt ggatccggcg ctgttgcgcc ccggccgctt
     4741 tgaccggcag gtggtggtgc cgcttcccga catccgcggc cgcgagcaga ttcttttggt
     4801 gcatatgcgc aaggtgccga ttgcacccga cgtgcgcgcc gacattctgg cgcgcggcac
     4861 gcccggattt tccggcgccg atttggccaa tctggtgaat gaagcggcgc tattcgccgc
     4921 gcggttcaac aagcgcctgg tggacatgga cgacttcgag cgcgccaagg acaaggtcat
     4981 catgggcgcc gagcgccgct caatggtgat gcccgagcat gagcgccgca ataccgctta
     5041 ccacgaatcg gggcatgcgg tggtggcgaa gcttctgccc aaaaccgacc cggtgcacaa
     5101 ggtcaccatc attccgcgcg gccgcgcgtt aggcgtgacc atgcagttgc cgcaggaaga
     5161 ccgctacagc ctcgaccgcg agcatatact gcagaatatt tcggtgctgt ttggcgggcg
     5221 tatcgccgag gaaatattca tggggcaaat gaccaccggc gcctccaacg atttcgaacg
     5281 tgccaccgaa atggcgcgca acatggtgac gcgctggggt atgagcgacg agctgggccc
     5341 gatggtgtac ggcgagaacg agggcgaggt gttccttggc cgttccatca ccacacacaa
     5401 aaatgtttcc gaaacgacga tgcagaaagt agacgccgaa atccgccgca tcatcgacca
     5461 gcaatatgcg ctggcgcgca agctgatcga ggaaaaccgc gacaaagtgg aagcgatggt
     5521 gaaagccctc ctggaatggg agaccatcga ctccgaccag atcaacgaca tcatggaagg
     5581 ccgcccgccg cggccgccca aaccggtaca gcctccgcaa cccagccagg acacgccccc
     5641 tggaggcgca accgctaccg cccagccggc ggcacgggaa acctgacatc aggggttagg
     5701 gctcaaggga ttagggaata ggaaaggctc tgaactcgaa ttttctagcc cctagcccct
     5761 agcctctagt ccctaacccc tatgtttctc cagtgcggta aattcaagct cgatttatcc
     5821 cgtcccctga tcatggggat agtcaacgtc acacccgatt cattttccga tggcggcaag
     5881 ttggtctcgg caccggccgc catcaatcac gccaaacaac tggtcgagga gggcgcggat
     5941 attctggaca tcggcggtga atccacccgt ccgggcgcgg aacccgtaag cctggaagag
     6001 gaattgcggc gggtattgcc ggttctggaa ggattaaaag acgttaatgt tcctctctcc
     6061 gtggacacct ataaacccga agtgatgcgg gcggcaattt ctgcaggagc atccatgatc
     6121 aatgatgtca acgctttgct ggcaccgggg gcgatggaat cggtagccgc atccgacatg
     6181 gccctgtgcc tgatgcacaa gaaaggcgaa cccaagacca tgcaacaaaa cccgcagtac
     6241 gaagatgtgc tcaccgaggt tgccgatttt cttaaggcaa gggtcgcggc ggcggaagcc
     6301 gcgggcgtgg ctcgggagcg catctgcata gatccgggca tcggttttgg caagaacctc
     6361 gagcataatc tggccttgct gaggaatatc ggccgcttga aatcgattgg tgtgccggtg
     6421 ttgatcggcg tgtcacgcaa atcgctattg ggaaaaatcc tcggactgga agtgtacgac
     6481 cgggtgcagg tgaacgcagt agcggcggtc cttacggtga gacaaggcgc ggcgatcgtg
     6541 cgagtacacg atgtcaaggc caccaaggat gcgcttgcgg tttataatgc ggtgggtaga
     6601 gaagcgtaac gcatccggat tcatcgtcat ggcaagaaaa tatttcggca ccgacggcgt
     6661 aaggggcaca gtgggcgaag cgcccatcac cccggatttt gtcatgcgtc tgggctacgc
     6721 ggcgggcaag gtgctcgcgg gcatgagcaa aagcacggcg gagcgcgccg cggtgctgat
     6781 cggcaaggac actcgcattt ccggctacat gctggagtct tccctcgaag ccggcctttc
     6841 cgcggcgggc gtggatgtgc tgctggtcgg gccgatgccg acaccggctg tcgcctatct
     6901 cacacgcgcg ctgcgactgt cggcgggcat cgtgatttcc gcctcgcaca atccgtataa
     6961 cgacaacggc atcaagtttt tctccggcac cgggctgaag ctggcggatg aaatcgaact
     7021 ggctattgaa accgagctgg aaaagccgct caactgcaat gcttcggcgc agctcggcaa
     7081 ggctaggcgc atcgacgacg ctgcgggccg ttacatcgaa ttctgcaaaa gcacgtttcc
     7141 ctccgatctc gacctgcgcg gcttgcgcct ggtggtggac accgcgcatg gtgcgactta
     7201 tcacattgcg tcccatgtgt ttcacgaact cggcgccgac gtgattgcga ttggcaacca
     7261 gccggacggc ttgaacatca atcaggaatg cggcgccgct tatcccgggt cgctgctgga
     7321 ggcggtcaag gaacatcgag ccgatcttgg tatcgcgctc gatggcgacg gcgaccgcct
     7381 gttgatggcg gacagtggcg gcaaaaccta tgacggcgac cagttgctct tcatcatcgc
     7441 caaacaccgc cagcagcagg gtacgctcaa gggcggcgtg gcgggaacgc tcatgaccaa
     7501 tctcggcatg cagcgcgcgt tggagaaact gcgggtaccg tttgcccgcg ccgccgtcgg
     7561 cgaccgttat gtgctggagc tcttgctgga aaaaggctgg cagcttggcg gggaaaattc
     7621 cggccatatc atctgcctcg acaagcacac caccggcgac ggtatcattt ccgctttgca
     7681 agtgctctgt gccttgcgcg cttacaacaa gggattggcc gagctcactc gtgatctcgc
     7741 gctctatcca caagtgctgg tgaacgtcaa agtcgtgaaa ggattcgatt tccgtgagca
     7801 accgccggtg aaagcggcgc tcaaccgggc cgaggcggat ctcggcgatg aaggccgggt
     7861 gctgttgcgc gcttccggca ccgagccggt ggtgcgagtg atggtggaag gccagccgca
     7921 gaacaaggtc aagcactggg ccgataccat cgcccaagcg gttcgcgaag ccgcagtgaa
     7981 ataacttttg gcttttttta tcttgaag
//
LOCUS       DATIOW010000076         8316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_844945, whole genome shotgun sequence.
ACCESSION   DATIOW010000076 DATIOW010000000
VERSION     DATIOW010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8316
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844945"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VLL03_02335"
     CDS             complement(<1..209)
                     /locus_tag="VLL03_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HSS46236.1"
                     /translation="MAKHFSLREFQQGLSARLQALANAPSADVSKLGVQAGGNLWLVD
                     LIECGEVVTLPELIKVPLTRPWFSGV"
     gene            complement(209..574)
                     /locus_tag="VLL03_02340"
     CDS             complement(209..574)
                     /locus_tag="VLL03_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS46237.1"
                     /translation="MAIKKILVVDDSPTDRHFFTVILTKSGYEVSTAENADEALAKAK
                     KLKPDLVLMDVVMPGQNGFQATRALNKDEATKSIPVIIVTSKNQETDKIWGMRQGAKD
                     YIVKPVDQAKLLAKIASLR"
     gene            complement(587..955)
                     /locus_tag="VLL03_02345"
     CDS             complement(587..955)
                     /locus_tag="VLL03_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS46238.1"
                     /translation="MVIDDSNTIRRSAEIFLLQAGCEVILAEDGFDALAKITDHLPDV
                     IFCDIMMPRLDGYQACALIKKNSRFKRTPVIMLSSKDGLFDRARGRMVGSDEYLTKPF
                     TKDSLLKTVGTHAQKHVVNS"
     gene            1245..2393
                     /locus_tag="VLL03_02350"
     CDS             1245..2393
                     /locus_tag="VLL03_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSS46239.1"
                     /translation="MKRLGANTKSWVLAVVLGASSPALAAEAGISDTTILVGQSAAFS
                     GPAAELGREMRDGAKAYFDLVNSEGGVFGRKIEMKSLDDGYEPDRAAANTKKLINEEK
                     VFALIAYVGTPTSAAALPIFTEAKVPFVGPFTGAELLRSPFNKYIFNVRASYYDETEK
                     IVEQLVSIGIKNIAVFYQNDNYGQAGLKGVEIAMKKRNIPISATGTVERNTIKVEDAV
                     KAITKVKPDAVVMVSAYKSCSEFIRQMKKAGSTAQFYNVSFVGSKALSADLGADGTGV
                     AISQVVPFPWSPSIPIVKEYQKVVSRFAPQSPYSFSTLEGYIAAKVFVEGLKKTGKDL
                     TREKFIAALEKIQDWDLGGFNVNFSPANHNASKFVDLTIIGKDGKFLH"
     gene            complement(2394..3176)
                     /locus_tag="VLL03_02355"
     CDS             complement(2394..3176)
                     /locus_tag="VLL03_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HSS46240.1"
                     /translation="MLDAVISAVREVAKQQIMPRYLKVAQQRKSDGSLYTDADIAAQE
                     ALALRLREIHASPVLAEEMSHEQQAEHWLAGEAGLWCVDPIDGTSNFVNGLPYFAVSV
                     ALMRKGKSVLGVIYDPVADEIFYAEKGRGAYLNGEKLPINESSRELHQCMASIDFKRI
                     GASLRQALAGSPPYFSQRNYGASTLEWCYVAAGRFDVYLHGGQKLWDYAAGSLILEEA
                     GGMMCSLKTDDFWADDLWNRSVIAARNPALFEAWKAWLRARQ"
     gene            complement(3200..3757)
                     /locus_tag="VLL03_02360"
     CDS             complement(3200..3757)
                     /locus_tag="VLL03_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HSS46241.1"
                     /translation="MVNLQTPVCDFGWKAVDFDLPGVDGKRYSLSNARGEKGLLVMFI
                     CNHCPYVKAVRSRIVRDCAELKQHGIGAIAIMSNDTADYPEDSFENMIKVAREFKFPF
                     PYVLDETQEAAKAYGAVCTPDFYGFNAKLKLQYRGRLDASRKDAAPDARRDLFEAMLQ
                     VARTGQGPLEQIASMGCSIKWKHDA"
     gene            3812..4540
                     /locus_tag="VLL03_02365"
     CDS             3812..4540
                     /locus_tag="VLL03_02365"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HSS46242.1"
                     /translation="MTIARFYHSGELASHREIFLDEAAAHHAARVLRLKEGDTMVLFN
                     GKGGEYPVRITGIVKTKVTVLTGEWQNIERESPLQIMLAQAISSADKMDLTVQKAVEL
                     GVSAIHPLATQRSVVRLPGERARKRVEHWRKVAIHACEQCGRNRIPQIGEIASLNDFL
                     GKAKADALRLALSPNGGISFKRLKPQDTVVLLVGAEGGFTQEEEQRARAAGFEPVALG
                     RRILRTETAGIAALAAMQTLWGDF"
     gene            4544..5467
                     /locus_tag="VLL03_02370"
     CDS             4544..5467
                     /locus_tag="VLL03_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HSS46243.1"
                     /translation="MYAIIVHGGAGAWEAEHKRAAVQGVKRAVQAGAQLLAQGKSALD
                     AVTAAVVSLEDNPVFNAGTGSVLNLEGEAEMDASVMVSKGLCSGGVTCLSRVKNPVRV
                     ARKVMEETEHVLLAGEGALNFARAMGFADHDPVTRERRADWQKKSRALSAVKNQSRLR
                     NLLKRHPELAGGTVGAVALDKRGDLAAATSSGGLTLKLPGRVGDTPILGAGNYAMPLG
                     AASATGLGEVAMRYLVTKSVCDLLAQGRNAQQAVDEVLSFLTAEPHADMGIITIDRRG
                     NIGAGHRSRAMPHAYCVSGRSKIVARMQVRP"
     gene            complement(5482..7317)
                     /gene="typA"
                     /locus_tag="VLL03_02375"
     CDS             complement(5482..7317)
                     /gene="typA"
                     /locus_tag="VLL03_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HSS46244.1"
                     /translation="MPRALRNIAIIAHVDHGKTTLVDKLLHQAGTFAAHQHVAERVMD
                     SNDLERERGITILAKNTAVNYQGTHINIVDTPGHADFGGEVERVLSMVDGVLLLVDAV
                     EGPMPQTRFVTRKALAQGLKFIVVVNKMDRPGARPDWVVNHTFDLFDKLGAAEQQLDF
                     PVIYASALNGWAVLDLADAPRSGAGEGLSMRPLFETILKHVPVPAGNPDGPLQLQISA
                     LDYSSFIGRIGIGRIRRGRLKAAQDVVILNGHDEPKKARVNQVLCFQGLTRVPMEIAE
                     VGDIVLVNGIEEIGIGATLTDPERPEALPLLAVDAPTLTMNFQVNTSPYAGQEGKFVT
                     SRQLRERLHKELLTNVALRVEDTADTDVFLVFGRGELHLTILLENMRREGYELAVSRP
                     RVVIREINGEKCEPFEMLTVDVEEANQGAVMEALGERRGELQDMAPDGRGRVRLDYRI
                     PARGLIGFQSDFMTMTRGTGILSHVFDGYAPMKPEIPERRNGVLVSAEDGGAVAYALW
                     KLQERGRMFVSPGDRLYEGMVIGIHSRDNDLVVNPIKTKHLTNIRAAGKDEAIMLTPP
                     IALTLERAVEFIADDELVEITPQTIRIRKRHLKENERKRASREAA"
     gene            complement(7499..>8316)
                     /locus_tag="VLL03_02380"
     CDS             complement(7499..>8316)
                     /locus_tag="VLL03_02380"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HSS46245.1"
                     /translation="AHARELIMEDGICRGVRYQDSNGEEHELRALLTVAADGRFSRLR
                     KLADLEPVKSSPPMDVLWFRLPKRPGDTLGFGGRIGQGHMLVILPREDHYQLGYVIFK
                     GGYHALREEGMEKFHQSLLSFAPEFADRVSSLQGWGQLSVLSVESSCLKQWHKPGLLF
                     IGDAAHVMSPVGGVGINYAIQDAVVAANVLTGPLRRGQVSESDLASVQKQRYFATRVI
                     QAFQTFIQNRVIKTALNPDVSFRPPALFRLPLLRALPARLIGFGIRKVHLEEK"
BASE COUNT         1732 a         2379 c         2458 g         1747 t
ORIGIN      
        1 acgccgctga accaaggccg ggtcaacggc actttaatga gctcgggcag cgtcacgact
       61 tccccgcact caatcaagtc caccagccac aaatttcccc ccgcttgcac gcccagtttc
      121 gagacgtccg cggacggagc attcgccagc gcttgtagcc gcgctgatag accttgctga
      181 aattctctca ggctgaaatg cttcgccatt acctaagaga ggcgattttc gccagcagtt
      241 tggcctggtc caccggcttt acgatgtagt cctttgcgcc ttggcgcatg ccccagattt
      301 tgtcggtttc ctggttcttg gaggtgacga tgatgaccgg aatgctcttg gttgcctcgt
      361 ctttattcag cgcccgcgtg gcctggaaac cgttttgccc gggcatgacc acgtccatca
      421 agaccaaatc gggtttgagc tttttcgcct tggccagtgc ttcatcggcg ttttccgcgg
      481 tggagacctc gtagccgctc ttggtcagga ttactgtgaa gaaatgccgg tctgtgggcg
      541 agtcgtcaac aaccaggatt ttcttgattg ccatgattta attccttcaa ctgttaacca
      601 cgtgtttctg tgcatgagtg cctaccgttt tcagcaagct gtccttggtg aaaggcttgg
      661 tcaggtactc atccgagccc accattctcc cgcgcgcacg gtcaaacagc ccgtccttac
      721 tcgacaacat gatgaccgga gtacgtttga acctggagtt tttcttgatc agggcgcagg
      781 cttggtagcc atccagccgt ggcatcatga tgtcgcaaaa aatcacgtcc ggcaggtgat
      841 cagtaatttt ggcgagcgca tcaaaaccgt cttccgccag tatcacctcg caaccggcct
      901 gcagcaggaa aatctcggcg ctgcggcgga tggtgttgct gtcatcaatc accatgacct
      961 tgagtcccac caggttgctg ccttccactt gatctcctcg gtctgcgcac caacttgctt
     1021 taatggtggt ttacagcgtg cttagagagt gcgaagataa aatagcattt cgcgaggtta
     1081 aacaccatac ctcatgctgt accttgagca taggcaggtt cgtgtttaat tctgaaatta
     1141 agtttcggcg gcaaacgcgc ctcaggcccg gcggtttcta gaatgggaag gcggtgccgc
     1201 aaaccgcagc atcgcggctg aatcaccagt ggaggagaag cactatgaag cgcttgggtg
     1261 caaatacgaa atcgtgggtg ttagccgtag tgctgggggc gagttcgcct gcgctggcgg
     1321 cggaggcggg tatcagcgat acgacaattc tagtcggaca atcggcagcc ttcagcggcc
     1381 cggcagcgga gctggggcgc gaaatgcgcg atggggccaa ggcttatttc gacctcgtca
     1441 acagcgaagg cggagtgttc ggccggaaaa tcgagatgaa atccctggac gacggttacg
     1501 agccggaccg cgccgcggcg aataccaaaa aactcatcaa tgaagaaaag gtgtttgccc
     1561 tgatcgccta tgtggggacg ccgacgagcg cagcggccct gccgattttt accgaggcca
     1621 aagtgccgtt tgtgggcccg tttaccggcg cggaactgct gcgcagtccg ttcaacaaat
     1681 acatcttcaa cgtgcgcgcg agttactacg atgaaacaga aaaaatcgtc gagcagttgg
     1741 tgtcgatcgg catcaaaaac atcgccgtgt tttaccagaa cgacaattac gggcaggcgg
     1801 gtttgaaggg cgtggaaatc gccatgaaaa aacggaatat cccgatttcc gcgaccggca
     1861 cggtggaacg caataccatc aaggttgaag atgcggtgaa agccatcacc aaggtcaaac
     1921 cggatgcagt ggtgatggtc agcgcctaca aatcctgcag cgagtttatc cggcaaatga
     1981 aaaaagccgg cagcaccgcc cagttttaca atgtttcctt cgtcggcagc aaggcgctgt
     2041 ctgccgacct gggcgcggac ggcacgggcg tggcgatttc gcaggtggtg ccgtttccgt
     2101 ggagcccgag cattccgata gtcaaggaat atcaaaaagt cgtgtcccgg ttcgcgccgc
     2161 aaagcccata ttcattctcc accctggaag gttatattgc cgcgaaagta ttcgtcgagg
     2221 gcttgaagaa gacagggaaa gacctgaccc gcgagaaatt cattgcggcg ctggaaaaga
     2281 ttcaggattg ggatttgggc ggctttaacg tgaactttag cccggccaac cacaacgcct
     2341 cgaagtttgt ggacttgacc atcatcggca aggacggcaa gttcctgcac taactactgc
     2401 cgcgcgcgca gccaggcttt ccaggcttca aacaaagccg ggttgcgtgc cgcgattact
     2461 gagcgattcc ataagtcgtc ggcccagaaa tcgtcggtct taagcgaaca catcatgccg
     2521 cccgcttctt cgagaatcag tgagccggcg gcgtagtccc atagtttttg cccaccgtgc
     2581 aaatatacat cgaagcgccc ggcggcgacg tagcaccatt ccagcgtgct cgcgccgtaa
     2641 ttgcgttgcg agaagtaagg cgggctgccg gcaagcgcct gccgcaatga ggctccaatg
     2701 cgcttgaaat cgatgctcgc catgcattgg tgcagttccc tgctggattc gttgatgggg
     2761 agcttctcgc cgttcaaata agcgccgcgg cctttttcag cataaaatat ttcatccgcc
     2821 accgggtcgt aaatcacgcc gagcacgctt ttgcctttac gcatcaacgc cactgacacc
     2881 gcgaaataag gcagaccgtt gacgaagttg gaggtgccgt caatcgggtc cacgcaccac
     2941 aggccggctt cacccgcgag ccaatgctcg gcttgctgct cgtggctcat ttcttcggcg
     3001 aggacaggac tcgcatgaat ctcgcgcagc cttagcgcga gtgcttcctg cgcggcgata
     3061 tcggcatcgg tatagaggct gccgtcggat ttacgctgct gtgcgacctt caggtagcgt
     3121 ggcatgattt gctgcttcgc gacttcgcgc accgcgctga ttaccgcatc aagcatcgcg
     3181 caccacacca attaccgcat caagcatcgt gtttccactt gatcgagcag cccatgctgg
     3241 ctatttgttc cagcggtccc tggccggtac gcgccacctg cagcatggcc tcaaacaaat
     3301 cgcgcctcgc gtcgggcgcg gcgtccttgc gcgaagcgtc gagccgtccg cggtattgca
     3361 acttcagttt ggcgttgaag ccgtaaaaat ccggcgtgca taccgcgcca taggctttgg
     3421 ccgcctcctg ggtttcgtcg agcacatagg ggaacgggaa cttgaattcg cgcgcgactt
     3481 tgatcatgtt ttcaaatgaa tcttcaggat aatcggcggt gtcattcgac atgatggcga
     3541 tagctccgat gccatgctgc ttgagctcgg cgcagtcgcg cacgatgcgg ctgcgcaccg
     3601 cttttacata ggggcaatgg ttgcagataa acatgaccag gaggcccttt tcgccgcgcg
     3661 cattcgataa cgagtaacgc ttgccgtcga ccccgggcag gtcgaaatcc accgctttcc
     3721 agccgaaatc gcacaccggt gtttgcaggt tcaccataaa gttgctccgt caatatatta
     3781 tcatgcatct gtttactaaa ccttccctca tatgaccata gcgcgctttt atcattccgg
     3841 cgaattggcc tctcatcgcg aaattttcct cgatgaggcg gcggcacatc acgccgcgag
     3901 ggtattgcgc ctcaaggaag gcgatacgat ggtgttgttc aatggcaagg gcggggaata
     3961 tccggtcagg attaccggca tagtcaaaac caaagtaacg gtactgacag gcgaatggca
     4021 aaacatcgag cgcgaatcgc cgctgcaaat aatgctggcg caagccattt cttccgccga
     4081 caaaatggat ttaaccgtgc agaaagcggt ggaattgggc gtgagcgcca ttcatccgct
     4141 cgctactcag cgcagcgtgg tcaggctccc cggcgagcgc gcccgaaagc gcgtggagca
     4201 ctggcgcaag gtggcgatcc acgcgtgcga gcagtgcggc cgtaaccgca ttccgcaaat
     4261 cggggaaatt gcttcgctga atgatttcct cggcaaggcg aaagccgatg cattgcggct
     4321 cgcgctctca cctaatggcg gaatatcttt caagcggctc aagccccagg atacggtggt
     4381 cctgctggtc ggagcggaag gcggattcac tcaagaagaa gaacagcgcg cccgcgccgc
     4441 cggttttgaa ccggtcgcgc tgggacggcg catcctgcgc accgaaaccg caggaatcgc
     4501 cgcgctggcc gcgatgcaaa cactgtgggg ggatttttaa cccatgtacg cgatcatcgt
     4561 tcatggaggg gcgggagcct gggaagcgga acataaacgt gcggcggtac agggcgtgaa
     4621 gcgcgcggtg caagcgggag cgcaattgct cgcgcaaggc aagtccgcgc tcgatgcggt
     4681 gaccgccgcg gtggtatccc tggaggacaa cccggtgttc aacgccggca ccggctccgt
     4741 gctgaatttg gaaggcgaag ctgagatgga cgcgagtgtt atggtgagca agggattgtg
     4801 ttcgggtggc gtcacctgcc tgagccgcgt caaaaacccg gtgcgggtgg cgcgcaaggt
     4861 catggaggaa accgagcatg ttctgcttgc gggcgaaggc gcgctcaact ttgcccgcgc
     4921 catgggattt gccgatcacg atccggtgac gcgagagcgg cgtgcggatt ggcaaaagaa
     4981 aagccgggcg ctgtcagcgg taaaaaacca aagccgcctg cgaaatttgt taaagcgcca
     5041 tcctgagctt gcgggcggga cggtaggcgc ggtggcgctg gataaacgcg gtgatttggc
     5101 tgcggcgact tcatccggcg ggttgacact gaagcttccg ggacgggtcg gtgatacgcc
     5161 cattttggga gcgggaaatt acgccatgcc tttgggcgcg gcatccgcca ccggcttggg
     5221 cgaagtggcg atgcgttacc tcgtgaccaa atcggtgtgc gatctgctgg cgcagggcag
     5281 aaatgcgcag caggccgtgg atgaagtgct gtcgttcctg acggcggaac cgcacgcgga
     5341 tatgggcatc atcaccatag acaggcgggg caacatcggc gcgggacacc gcagccgggc
     5401 catgccgcac gcttactgcg tttcgggaag aagcaaaatt gttgcgagga tgcaggtcag
     5461 gccctaaagc tggtcagcct gttaggcggc ttcgcgcgaa gcccgtttgc gctcgttttc
     5521 tttcaaatgg cgcttacgga tgcggatggt ttggggggtg atttccacca gttcgtcgtc
     5581 tgcgataaac tcgacggctc gctccagggt aagcgcaatt ggcggcgtga gcatgatcgc
     5641 ctcgtccttg ccggcggcgc ggatgttggt aagatgcttg gtcttgatgg gattcaccac
     5701 cagatcgttg tcccggctgt gaatgccgat caccatgccc tcgtacaggc gatcaccggg
     5761 gctcacgaac atccggcccc gctcctgcag tttccacagc gcgtaggcta ccgccccgcc
     5821 gtcttcggcc gacaccagga caccgttgcg ccgctcgggg atttccggtt tcatcggggc
     5881 gtagccgtcg aacacgtggc ttaagatacc ggtgccgcgg gtcatggtca tgaaatccga
     5941 ttggaagccg atcaatcccc gcgccggaat ccggtaatcg agacgcacgc ggccccggcc
     6001 gtcgggcgcc atgtcctgaa gctcgccgcg ccgttcgcct aaagcttcca tgaccgcgcc
     6061 ctggttggcc tcttccacat ccaccgtgag catttcgaac ggctcgcatt tctcgccgtt
     6121 gatttcccga atcacaaccc gcgggcggga caccgccagt tcgtaacctt cccggcgcat
     6181 gttttccagc aggatggtga gatgcaattc accccggccg aagaccagga acacatccgt
     6241 gtccgcagta tcttcgacgc gcagcgccac gttggtcagc aactccttgt gcagccgctc
     6301 gcgcagttgg cggctggtca cgaattttcc ttcctgtccg gcatagggtg aagtgttgac
     6361 ctggaaattc atggtgagcg tgggtgcgtc caccgccagc aggggtaagg cttccgggcg
     6421 ttccgggtcg gtgagggtcg cgccgatgcc gatctcctcg atgccgttca ccagtacgat
     6481 gtcgccgact tccgcgatct ccatgggcac gcgggtcaag ccctggaagc acagcacctg
     6541 gttcacccgc gcttttttcg gctcgtcatg accgttcaaa ataacaacgt cctgggcggc
     6601 cttaagacgc ccgcggcgga tgcgcccgat gccgatgcgc ccaatgaagc ttgagtaatc
     6661 cagcgcactg atttgaagct ggagcggccc gtcgggattg ccggcgggaa ccggtacatg
     6721 tttgagtatg gtttcgaaca gcggccgcat ggatagcccc tcacccgcgc cgctgcgcgg
     6781 cgcatcagca agatcgagca ccgcccaacc attgagcgcg gaggcataga tgacgggaaa
     6841 atccagttgc tgctcggcgg cgcccagttt gtcgaataaa tcaaaagtgt ggttcaccac
     6901 ccagtccggg cgggcgccag ggcggtccat cttgtttacc accacgataa acttcaaacc
     6961 ttgggccagg gccttgcgcg tcacaaaacg ggtctggggc atgggcccct ctactgcatc
     7021 caccaggagc agcacgccgt ccaccatcga cagcactcgc tccacttcgc cgccgaaatc
     7081 cgcatgacct ggcgtatcga cgatgttgat gtgggtgcct tggtaattta ccgccgtgtt
     7141 cttggcgaga atggtgatgc cgcgctcccg ctccaaatca ttgctgtcca tgacccgctc
     7201 agccacgtgc tggtgggcgg caaatgtccc cgcttggtgg agcagcttgt ccaccagcgt
     7261 ggtcttgccg tggtccacgt gggcaatgat ggcgatgttg cggagtgcgc ggggcatggg
     7321 ggaactaacg gtttgacaaa agtcgcgcat tgtaacacag agaatccagg cagcccgcat
     7381 gaatcatgcg gagtcatggg cagtttgatt cggaataggc tgtttgccta tgaattcatt
     7441 cgttttatgt tttaccgtgt accgtttgat gcggggctga tgaaattcgt ctgcggccct
     7501 atttttcttc aagatgcacc ttgcggattc caaagccgat gagccgggca ggcaaggcgc
     7561 gcagcaaagg caggcgaaac agcgcgggcg gcctgaacga aacatcggga ttcaatgctg
     7621 ttttaatcac ccggttttga atgaaggtct ggaaagcctg aattacacgg gtggcaaagt
     7681 aacgttgctt ttgcaccgag gccaaatcgc tttcgctgac ttggccgcgc cgcaacgggc
     7741 cggtgagcac attggccgcc accactgcat cctgaatcgc gtaattgatg cccactccgc
     7801 ctaccggcga catgacatgc gccgcatcgc cgatgaacag caatccgggt ttgtgccatt
     7861 gtttcaggca acttgattcc accgaaagca ctgaaagctg gccccagcct tgcagggagg
     7921 atacccggtc ggcgaattcg ggggcgaagg acaacaggga ctggtgaaat ttttccatgc
     7981 cctcctcgcg gagcgcgtga tagcctccct tgaaaatcac gtagccgagt tggtaatggt
     8041 cttcccgcgg caggatgaca agcatatgtc cttgcccgat gcggccgcca aaacccaggg
     8101 tatcgcccgg ccgcttgggc agccggaacc ataacacgtc catcggcggg gaactcttca
     8161 ccggttccag gtccgcaagt tttctgagcc tggaaaaacg accatcagcg gccacggtga
     8221 gcaacgcccg caattcatgt tcctccccgt ttgaatcctg atagcgcaca ccgcggcata
     8281 tcccgtcctc catgatcagc tcgcgggcat gggcgc
//
LOCUS       DATIOW010000077         7940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_437475, whole genome shotgun sequence.
ACCESSION   DATIOW010000077 DATIOW010000000
VERSION     DATIOW010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7940
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437475"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1333)
                     /locus_tag="VLL03_02385"
     CDS             complement(11..1333)
                     /locus_tag="VLL03_02385"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HSS46246.1"
                     /translation="MAQMLNKSVILAQKAARLTPDVRLRWLVALLTLPLFGVAAFGIA
                     PQTEVSKIPQQTVIEELRLPEFPQDGAQTGEYWREEHIQRGDTIASLLARLDVDEQET
                     LKLLRHPKELKPLRQLRPGKTVQAQVSDEGDLLLLRYFNANGGIFVAKKTGDHFAFGV
                     EPLSLEKRVLIKSGEIVSSLFAATDAVDLPDSIATQIAEIFSSDIDFYRDLRKGDRFS
                     VVYEMFYDQGTAVGSGRVIAAEFVNQGKAYQALYFNDQQGHGGYYTPDGKNMRKAFLR
                     SPLEFSRVTSGFSFSRFHPLLKEWRTHKGIDYAAPVGTKIRATASGTVVFFGKEGGYG
                     NMVVLRHQGVYSTVYGHLSAFAKGLHHGQKVSQGDVIGYVGMTGLATGPHVHYEFRIN
                     GVQHNPVHIAVPEGPPITAQLKPAFNSTVEPLQSQLNLARGVNFAKLD"
     gene            1410..2609
                     /gene="tyrS"
                     /locus_tag="VLL03_02390"
     CDS             1410..2609
                     /gene="tyrS"
                     /locus_tag="VLL03_02390"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HSS46247.1"
                     /translation="MSTIKQALAIIKRGYDELLIEEELEQKLKTGKPLRVKAGFDPTA
                     PDLHLGHTVLLNKLRQLQDLGHQALFLIGDFTGMIGDPTGKNATRPPLSREEIARNAK
                     TYSAQVFKILKREQTEVVFNSSWMDKLKAADMVKLAATYTVARMLERDDFAKRYKANQ
                     AIAIQEFLYPLVQGYDSVALKADLELGGTDQKFNLLMGRELQKHFGQPPQCILTMPLL
                     AGLDGVHKMSKSLGNTVGITEPPREIFGKLMSISDELMWRYIELLSFEPLEAIARWKK
                     EVEGGRNPRDIKVIFAQEIVVRFHGKKAAQDTLADFEARFKQGAIPDDVSETRLHAGS
                     DGMPIVQILKQTGLTASTSEALRLIEQGGVKLNGEKVADKSLNLHQGETAVLQVGKRK
                     FVRVTLT"
     gene            2646..3629
                     /locus_tag="VLL03_02395"
     CDS             2646..3629
                     /locus_tag="VLL03_02395"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HSS46248.1"
                     /translation="MKPLTFAVLRLLADGDFHSGEDLARGLGMSRASVWNALKEADQL
                     GVALYKIRGRGYRLAQPPQWLDQEKIAAALGAQANRFSLEIIDHTDSTNTLLLRKTAG
                     GAPKGSVVAAEWQTHGRGRRGRSWHMNLGGGLTFSMLWRFEQGAGFLSGLSLAVGVAL
                     ARALKSMGVEEIALKWPNDVLHHHRKLAGILIEVQGDVLGPSLAVIGIGINLRLSETV
                     RSRIDQAVTDVYSATRIAPDRNRVLGSVLTHLAQVLDFFEARGFAGVREEWSSLHAYH
                     RKNVLVMLPDGGVEEGKVIGVDDNGSLLLLASGGERRYNSGEISLRGKRAA"
     gene            3626..4345
                     /locus_tag="VLL03_02400"
     CDS             3626..4345
                     /locus_tag="VLL03_02400"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HSS46249.1"
                     /translation="MILAIDAGNSRIKWGWHDGRAWLKSGAAAHAEIPSLHHAWQALP
                     PPQTVVIANVAGEKIRLLLEPVLSGFNAKTHWIESRAVQCGVRNGYEAPAQLGADRWA
                     AAIAAWHLFRQACIIVNAGTAVTVDALSHNGVFLGGLILPGTSLMREALAQNTALNFP
                     RGRFRRFPANTADAIESGILQALLGAVERMGAALGGEATCLLSGGAALELAPHLNPPP
                     KVVDNLVLEGLVLIAGELNKK"
     gene            4342..5034
                     /locus_tag="VLL03_02405"
     CDS             4342..5034
                     /locus_tag="VLL03_02405"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HSS46250.1"
                     /translation="MKWLFYLLLIANAVIFAYVRLGAGNGDAQPTHQPFNADKIKLLR
                     EVPAAPALKPVAKLETAITTMAAKPAVCLEWGSFAGEEQARAQRALEKLQLGEKLGSR
                     SVEEGATYWVYIPPLKTRKDAEKKIAELKARGVTEYFLVQDGPAKNAISLGIFKSEEL
                     ANNYLASLQTKGVLTAIINERTHAAKRALFQIRDADEQAAIKLGELQRDFPGSEVRTI
                     ECPRLEAAAAAR"
     gene            complement(5037..6404)
                     /locus_tag="VLL03_02410"
     CDS             complement(5037..6404)
                     /locus_tag="VLL03_02410"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HSS46251.1"
                     /translation="MLPDKFLHCLKALLPAESLYADPVDCYAYSYDNTRKIFPPEAVA
                     FPSEAAQVREIIQLCNQHKIPVTARGRGTGTAGGSLPEKGGVALSLERMRRILSLDPA
                     NRVIVVEPGVLNQEVQDAVKAYGFFWPPDPSSAAYCSVGGNLATSAGGPHAVKYGTTR
                     DHVLGLKAVTGTGEIVKTGCYTTKGVVGYDLTRLIIGSEGTLAVITEATLKLTPLPQA
                     TGGFTAHYRDVASCAQAITAIMAQPQVPSALEFLDAGSLELIRAHHPQLVPDDTRAML
                     MIEVDGSVRETAAAREALLPACQNSGLLKAQVVDDASSLWAARKALAPLLRNIAPKKI
                     NEDIVVPVSRLPELLQGLSLLSAKYQVANVNFGHAGNGNIHVNLLVEPGDREQMRRAE
                     TCLNEVFDLVLSLNGTLSGEHGVGSEKRAFVAKEIDAATLALMKSIKRVFDPNNILNP
                     GKLFP"
     gene            6557..6694
                     /locus_tag="VLL03_02415"
     CDS             6557..6694
                     /locus_tag="VLL03_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46252.1"
                     /translation="MLLAAFYVLAITLVILHYTGWLAKHNLEWLLYVLALSVFPAVIF
                     L"
     gene            complement(6663..7190)
                     /gene="rfaE2"
                     /locus_tag="VLL03_02420"
     CDS             complement(6663..7190)
                     /gene="rfaE2"
                     /locus_tag="VLL03_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HSS46253.1"
                     /translation="MYPAPDFEKKVCEPELLASRIQRLPRPLVFTNGCFDLLHRGHAT
                     YLAQARALGASLVVGVNSDASVKRLSKGAGRPVNTLQERMAVLTALESVNLVTWFDDD
                     TPLKLIRACKPDVLVKGGDWKAENIVGAREVSGWGGKVHSIPFLYQTSSTAVLEKIRS
                     RRPAAVKEKSRREKR"
     gene            7340..7438
                     /locus_tag="VLL03_02425"
                     /pseudo
     CDS             7340..7438
                     /locus_tag="VLL03_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009458783.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
     gene            complement(7447..>7940)
                     /locus_tag="VLL03_02430"
     CDS             complement(7447..>7940)
                     /locus_tag="VLL03_02430"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSS46254.1"
                     /translation="GGVLVAEDRRTNAAMLEDESLEFKVCGRIIEQYNSQRDKLHLNV
                     TSYNRNVLITGEVPSDEVKAGAEKTAREVPNVRNVTNELVVGEPASFGSRSNDTLTTS
                     KVKTRFIEARKFQPNWVKVVTENKVVYLMGIVNHKEAADAADIASTTSGVEKVVKVFE
                     YTD"
BASE COUNT         1641 a         2224 c         2401 g         1674 t
ORIGIN      
        1 ggaggacaaa ttaatccagc ttggcaaagt tgacgccgcg cgccaggtta agctgtgact
       61 gcaagggctc gactgtgctg ttaaacgccg gcttgagttg ggcggtgatc ggcggcccct
      121 cggggacggc gatatgcacc gggttgtgct gcacgccatt aatgcggaat tcatagtgca
      181 cgtgcggccc ggtggcgagg ccggtcatgc caacgtaacc gattacgtcg ccctgactta
      241 ctttttgccc gtgatgcagg cccttggcga acgccgaaag atggccgtac accgtgctgt
      301 acacgccctg atgccgcaaa accaccatgt taccgtagcc gccctccttg ccgaagaaaa
      361 ccacggtgcc gctggcagtg gctctgattt tggtgccgac cggcgcggca taatcaatcc
      421 ccttgtgtgt gcgccattcc ttcagcagcg ggtggaagcg cgagaaacta aagcctgaag
      481 taacgcggga aaattccagc ggagagcgca aaaacgcctt gcgcatgttt ttgccatccg
      541 gcgtgtaata accgccgtgg ccttgctggt cgttgaaata caacgcctga taggctttgc
      601 cctggtttac aaattccgcc gcaatcacgc gcccgcttcc taccgccgtg ccctggtcgt
      661 agaacatttc gtaaaccaca ctgaagcgat cgcccttgcg caggtcgcga taaaaatcaa
      721 tgtcggagga aaaaatttcg gcgatctgcg tggcgatgct gtcgggaaga tctacggcat
      781 cggtggcggc aaacagcgag ctcacgattt cgccggattt gattaaaacc cgcttttcca
      841 gggagagcgg ctctacgccg aaagcgaaat ggtcgccggt ttttttcgct acgaaaatgc
      901 cgccgttggc gttgaaatag cgcagcagaa gcaggtcgcc ctcatcgctg acctgggctt
      961 gcacggtttt gccgggcctg agctgcctca acggtttcag ttctttggga tggcgcagga
     1021 gcttaagtgt ttcctgctcg tccacgtcaa gccgcgccaa caggctggcg atggtgtcgc
     1081 cgcgttggat gtgctcttcg cgccagtatt ccccggtttg cgccccgtcc tgcgggaatt
     1141 cgggcagccg cagctcctca atcacagttt gctggggaat tttgctcacc tcggtttgcg
     1201 gcgcaatgcc gaacgccgcc actccgaaca gcggcagagt aagcagggcc accagccagc
     1261 gcagcctgac atcgggtgtg agccgcgcag ccttttgcgc tagaatcacg cttttattaa
     1321 gcatttgtgc catgggtagt aaaagccagg cgccagtgta gcaaaaaacc agtagttggc
     1381 aaatgagcta ttcccgattt gagggtggaa tgagcacgat caagcaagcc ttggcaatca
     1441 tcaaacgcgg ctacgatgaa ttgctgatcg aagaagagct ggagcagaag ctcaagaccg
     1501 gcaaaccttt gcgggtcaag gcaggctttg atcccaccgc gccggatttg catctggggc
     1561 acacggtact cctcaacaag ctgcgccagt tgcaggacct cgggcatcag gcgctgtttc
     1621 tgatcggcga ttttaccggc atgatcggcg atcccaccgg caagaacgcg acccgcccgc
     1681 cgctttcgcg cgaggaaatc gctcgaaacg ctaagaccta ttcagcgcag gtgttcaaaa
     1741 tcctcaagcg cgagcaaacc gaagtggtgt tcaactcgag ctggatggac aaactcaagg
     1801 ccgccgacat ggtcaagctc gcggcgacgt atacggtggc gcgcatgctt gagcgcgacg
     1861 attttgcgaa gcgctacaaa gccaaccaag ccatcgccat acaagaattc ctttatccgc
     1921 tggtgcaggg ctacgattcg gtggcgctga aggcggatct ggaattgggc ggcaccgacc
     1981 agaagttcaa cctgctgatg ggacgcgaac tgcagaaaca tttcgggcag ccgccgcaat
     2041 gcattcttac catgccgctt ctggcgggac tcgacggtgt ccacaagatg tcgaaatccc
     2101 tgggcaacac tgtcggcatt accgagccgc cgcgggaaat ttttggcaaa ctgatgtcga
     2161 tctccgatga gctcatgtgg cgttacatcg agttgctctc gttcgagccg ctcgaagcca
     2221 ttgcgcgctg gaaaaaggaa gtcgagggag gtcgtaaccc gcgcgatatc aaggtaatct
     2281 tcgcccagga aattgtggtg cgcttccacg gcaaaaaagc agcgcaggac acgctcgccg
     2341 atttcgaggc gcgcttcaag cagggcgcca ttcccgatga tgtttccgaa acccggctgc
     2401 atgctggaag cgacgggatg ccaatcgtgc aaatcctgaa acagacgggt ctcaccgcca
     2461 gcacctccga agcgctgcgc ttgatcgagc agggcggggt gaaactcaat ggcgaaaaag
     2521 tggccgacaa atcgcttaat ctccaccaag gcgaaaccgc ggtgctgcaa gtgggcaaac
     2581 gcaaattcgt ccgggtcact ctcacctgaa acgcattctt tgcccgctgt cccgccaagg
     2641 cattcatgaa acccttgacc tttgccgtgt tgcgcctgct tgccgacggc gatttccatt
     2701 ccggcgagga tttggctcgc gggcttggca tgtcgcgcgc gagcgtctgg aacgcattaa
     2761 aggaagcgga tcagttgggc gtggcgctat acaaaatccg cgggcgcggc tatcgcctgg
     2821 cccagccccc gcaatggctg gatcaagaaa aaatagcggc tgcgctgggc gcgcaggcaa
     2881 acagattcag cctggaaatc atcgaccaca ccgattccac caacacgctg ctgctgcgaa
     2941 aaaccgcggg gggagcgccg aagggtagcg tggtggcggc ggaatggcag actcacggcc
     3001 gcggcaggcg cggacgcagc tggcacatga acctgggcgg tgggctgact ttttccatgc
     3061 tgtggcgttt tgaacaaggc gcgggttttc tttcgggttt gagcctcgcc gtaggggtgg
     3121 cgctggcgcg ggcgctgaag tccatgggcg tggaagaaat tgcgttgaaa tggccgaatg
     3181 atgttcttca tcatcaccgg aagcttgccg gcattctcat cgaagtgcag ggtgatgtgc
     3241 tggggccgag ccttgcggtt attggcatcg gcataaactt gaggctttcc gaaacggtga
     3301 gaagccgcat tgaccaggcg gtgactgatg tttattccgc aacccggatt gctcccgacc
     3361 gcaatcgggt attgggaagt gtgcttacgc atctggcgca agtgctggat tttttcgagg
     3421 cgcggggatt tgccggggtg cgcgaagagt ggtcgagcct gcacgcctat catcgcaaga
     3481 atgtgctggt catgttgcct gatggcggcg tcgaggaagg caaggtaata ggcgtcgacg
     3541 acaatggctc gctgctgctt ttggccagcg gcggcgagcg ccgctataac agcggcgaaa
     3601 tcagcctgcg gggaaaacga gccgcatgat actcgcaatc gacgctggca acagccgcat
     3661 caaatggggt tggcacgacg gccgcgcctg gcttaaatcg ggcgccgccg cgcatgcgga
     3721 aatcccgagc ctgcaccatg cctggcaagc gttgcctccg ccccaaaccg tcgtgattgc
     3781 caatgtcgcc ggagaaaaaa tccgcctgct gctggaaccg gtcctgtcgg gtttcaacgc
     3841 caaaacacac tggattgaat cgcgtgctgt tcagtgcggc gtgcgcaatg gttacgaagc
     3901 gcctgcgcaa ttgggcgctg accgctgggc ggccgccatt gctgcctggc acctattccg
     3961 gcaagcctgc attatcgtca atgccggcac ggctgtgact gtcgacgcct tatctcataa
     4021 cggcgtattt ttgggtgggt tgattcttcc tgggacttcg ctgatgcggg aagctttggc
     4081 gcagaacacc gcgctcaact ttccgcgcgg gcgattccgc aggttcccgg ccaacaccgc
     4141 ggacgcgatt gagagcggca ttctgcaggc gcttttaggc gcagtggagc gcatgggcgc
     4201 ggcattgggc ggggaagcca cgtgcctgtt gagcggcggc gccgcacttg agcttgcccc
     4261 gcatcttaac ccgccgccga aggtggtgga taatctggta ctggaaggtt tggtcctcat
     4321 cgccggggag ctcaataaaa aatgaaatgg ctgttttacc tgttgctgat tgccaatgcc
     4381 gttatttttg cctacgtcag gctgggtgca ggcaatggcg acgcgcagcc gacacatcag
     4441 ccgttcaatg ccgacaagat caagctgctc cgggaagtgc cggccgcgcc ggctttgaag
     4501 cccgttgcga aactggaaac agcgattacc acgatggcgg ccaaacccgc cgtatgcttg
     4561 gaatggggaa gctttgccgg cgaggaacag gcgcgtgcgc agcgggcgct agagaaactg
     4621 caactggggg aaaaactggg aagccgcagc gtggaagaag gcgctaccta ctgggtgtac
     4681 atcccgccgt taaaaaccag aaaggacgcg gagaaaaaaa tcgccgagct gaaagcacgc
     4741 ggcgtgacag aatatttcct ggtgcaggac ggcccagcga agaacgccat ttcacttgga
     4801 atattcaaaa gcgaggaatt ggccaacaac tacctcgcca gtttgcagac caagggcgtg
     4861 ctcacggcca ttattaacga acgcacccat gccgcgaagc gcgccttgtt ccagattcgc
     4921 gatgcggacg agcaagccgc aatcaagctt ggtgagttgc agcgtgattt tccgggaagt
     4981 gaagtcagga ctatcgaatg ccccaggctc gaagcggctg cagcggcacg ctagttctac
     5041 gggaagagtt ttcccgggtt gagaatattg ttgggatcga acacgcgttt gatgcttttc
     5101 atcagcgcga gcgtcgccgc atcgatttcc ttcgccacga atgcacgctt ttcgctaccc
     5161 acgccatgct cgccggaaag cgtgccgttg aggctcagca ccagatcaaa gacttcgttc
     5221 aggcaggttt cggcacgccg catttgttcg cgatctcccg gttcgaccaa aaggttcaca
     5281 tggatgttgc cattgccggc atggccaaaa ttgacgttgg cgacttgata tttcgcgctt
     5341 aacaatgata ggccctgcag caattccggc aatcgggaaa ccggcactac aatatcctcg
     5401 ttgattttct tcggcgcgat gttgcgcaaa agcggcgcaa gcgctttgcg cgccgcccat
     5461 aatgacgaag catcgtccac gacctgcgct ttaagcaggc cggagttttg gcaagccggc
     5521 agcagggctt cccgcgccgc cgcggtttcc cgcaccgagc cgtccacttc tatcatcagc
     5581 atggcgcgag tatcatccgg taccagctga gggtgatgcg cgcgaatcag ctcgagcgag
     5641 cccgcatcga gaaactccag ggcgctgggc acctgcggct gtgccatgat ggcggtaatc
     5701 gcctgcgcgc agcttgcgac atcacggtag tgggcggtga agccgccggt ggcctgcggc
     5761 agcggcgtaa gcttgagcgt cgcttcggtg atgacggcaa gcgtgccttc ggaaccgatg
     5821 atgagccggg taaggtcgta gcccactacg cccttggtgg tgtagcagcc ggttttgacg
     5881 atttctccgg tgccggtcac cgccttgagg ccgagcacgt ggtcgcgggt ggtaccgtat
     5941 ttcaccgcgt gcggcccgcc agcgctggtg gcgagattgc cgcccacgct gcaataagcg
     6001 gcgctggaag gatccggcgg ccagaagaaa ccgtacgctt ttactgcatc ctgcacttcc
     6061 tgattcagca cgccgggctc aaccacgatg acgcgatttg ccgggtcgag ggaaagaatg
     6121 cggcgcatgc gttccagcga cagcgcgacc cccccttttt ccggcaggct gccgccagcg
     6181 gtgccggtgc cgcgcccgcg cgccgtcacc gggattttat gttgattgca gagttgaatg
     6241 atttcccgga cctgcgccgc ctcgctggga aaagccaccg cctcgggcgg gaaaatcttg
     6301 cgcgtgttgt cgtaggaata ggcgtagcag tccaccgggt cggcgtaaag gctttccgcg
     6361 ggaagcagcg ctttcaggca gtgtaggaat ttgtcgggga gcatccggtc tgcgagctcg
     6421 ggagtcgagg taaaattata gcaactacga cggtgtttga ccgccggaaa cgccccgcga
     6481 aggtgtaatt tttgacattt cttttgaaag gaaaaggctt tatacttggt ttggaggaaa
     6541 ttttaagggg gagcttatgc tgcttgctgc gttctatgta ttggcgatta cgctggttat
     6601 cctgcattac accggctggc tcgccaagca taacctggaa tggttgctct atgttctggc
     6661 gctcagcgtt tttcccgccg tgatttttct ttaactgcgg ccggccggcg gctacggatt
     6721 ttttcaagca cagcggtaga ggaagtttgg tagaggaaag gaattgaatg aactttcccg
     6781 ccccagcctg aaacttccct cgctccgacg atattttcgg ctttccagtc cccacctttt
     6841 accagcacat ccggcttgca cgcgcgtatc agcttgagcg gcgtgtcatc atcgaaccag
     6901 gtgacaagat tcaccgattc cagcgccgtc agcaccgcca tgcgctcttg cagcgtgttg
     6961 accggacgtc cggccccttt gctcaagcgt tttaccgagg cgtcggaatt gacgccgacc
     7021 acgaggcttg cgcccaaagc gcgagcctgg gcgaggtagg tggcatggcc gcgatgcaga
     7081 agatcgaagc agccgttggt gaacaccagc ggccgcggca accgctgaat tcgtgaagca
     7141 agcagctcag gctcgcaaac cttcttttca aagtcaggag cgggatacat ggcttagggc
     7201 gtttttggag ctctaagtgt atcacgccgc tctggtgact tcctcgcagt aacggttgaa
     7261 ttcctgcacc gtgttgaagg tctggttaaa caggctcgaa aggttgcgca ggatcccacc
     7321 caccgtctcc cattccttcg gccgcgttaa ctatcaccgc ggcgattact gggacgcagc
     7381 agaagagctg tatgaaaaac tgccgctcat tggcagcccg atgcactggc ttaaatgacg
     7441 ggcgggctag tcagtatatt cgaatacctt gaccactttt tccacaccgc tggtggtgct
     7501 ggcgatgtcg gcggcgtcgg ccgcttcctt gtggttcacg atgcccatga gatataccac
     7561 tttgttttcc gtcactacct tgacccagtt cggctggaat tttctcgctt caataaaccg
     7621 cgttttcacc ttggaggtag tcaaggtgtc gttgctgcgc gagccgaaag aagcgggttc
     7681 gcccacgaca agttcgttgg tgacgttgcg cacgttcgga acttccctgg cggttttttc
     7741 ggcgccggcc ttgacctcat cggaaggtac ttcgccagta atcagcacgt tgcggttata
     7801 actcgtgaca ttgaggtgga gtttgtcgcg ctggctgttg tattgttcga tgatgcgccc
     7861 gcacaccttg aattcaaggc tttcgtcttc gagcatcgca gcgttggtgc ggcggtcttc
     7921 ggcgacgagc acgccgccgg
//
LOCUS       DATIOW010000078         2865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_133898, whole genome shotgun sequence.
ACCESSION   DATIOW010000078 DATIOW010000000
VERSION     DATIOW010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133898"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VLL03_02435"
     CDS             complement(<1..237)
                     /locus_tag="VLL03_02435"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701558.1"
                     /note="catalyzes the formation of dUMP from dUTP; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HSS46255.1"
                     /translation="MKKIDVKILDLRIKDQFPHYATPGSAGLDLRACIDSALTLKPGQ
                     AELIPSGIAIYLDDPGLAAIILPRSGLSHKHGIVL"
     gene            complement(234..1466)
                     /gene="coaBC"
                     /locus_tag="VLL03_02440"
     CDS             complement(234..1466)
                     /gene="coaBC"
                     /locus_tag="VLL03_02440"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HSS46256.1"
                     /translation="MVNMTQTKRLLLGVTGGIAAYKAAELTRLLVQDGIEVQVVMTEA
                     ACRFVGPATFQALSGNTVYTDLWDARIANNMAHIDLSREADVVLVAPASADFIAKLVH
                     GRADDLLSTLCLARECPLLVAPAMNRQMWENPATQRNIAQLEQDDVTILGPESGEQAC
                     GETGMGRMLEPETLLEDIRASFQPRLLQGKHVLITAGPTFEAIDAVRGITNLSSGKMG
                     YATACAALEAGAEVTLVSGTVSLAAPARVKLISVTSAQEMFAAVRQAAGDADIFISVA
                     AVADYHVVNPSTQKIKKGGQTLNLQLAPNPDILEYVASLPDGPFCVGFAAESENLYQH
                     AEQKRRDKKIPLLAANLVQDTLGKDESELVLFDDSGAHPLPRAPKTTLARQLIRRIAE
                     LYAKSRTVAQFKKNQAGK"
     gene            1539..2213
                     /gene="radC"
                     /locus_tag="VLL03_02445"
     CDS             1539..2213
                     /gene="radC"
                     /locus_tag="VLL03_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HSS46257.1"
                     /translation="MGISHWPEGERPREKLLSKGAQSLSEAELLAIFLRTGVAGKSAV
                     DVARGLIKRFGSLTRLFNASQKELCEAPGLGPAKYAQLRAVLEMARRTLDEKIKAGNA
                     LNSPKAVRDYLLLALSGREYEVFAGIFLDAQNRVLEMEELFRGTLSQTSVFPREVVKR
                     ALHHNAAGVIFAHNHPSGLAEPSGADEALTAALKKSLALVDVKVLDHFIVGSGGIMSF
                     AERGLL"
     gene            2305..2532
                     /gene="rpmB"
                     /locus_tag="VLL03_02450"
     CDS             2305..2532
                     /gene="rpmB"
                     /locus_tag="VLL03_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HSS46258.1"
                     /translation="MARVCEVTGKTPMLGNRVSHANNKTRRRFLPNLQYRKFWVESEK
                     RWVHLRLSNAALRTIDRKGIEAVLAELRGKK"
     gene            2544..2699
                     /gene="rpmG"
                     /locus_tag="VLL03_02455"
     CDS             2544..2699
                     /gene="rpmG"
                     /locus_tag="VLL03_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HSS46259.1"
                     /translation="MREKIKLESSAGTGHFYTTTKNKRNQTDKMEIKKFDPVARKHVI
                     YKEVKLK"
BASE COUNT          601 a          789 c          857 g          618 t
ORIGIN      
        1 gagcacgatg ccgtgcttgt gactgagccc cgagcgcggc aggatgattg cggcaagccc
       61 cgggtcatcc agataaatgg cgatgccgga aggaatcagt tcggcctgtc ccggcttgag
      121 ggtaagggct gagtcaatgc aggcgcgcag atcgagaccg gcggaaccgg gagtggcgta
      181 gtggggaaac tggtctttga tgcgcaggtc gaggattttg acatcaatct ttttcatttt
      241 cccgcttggt ttttcttgaa ctgcgcgacc gtgcgcgact tggcataaag ctccgcgatg
      301 cggcgaatca gttgccgggc gagcgtggtt ttaggcgcgc ggggcagggg atgggcgccg
      361 ctatcgtcga ataacaccag ttcgctttca tccttgccca gcgtgtcctg caccaggttc
      421 gcggcgagca gcgggatttt tttgtcgcgg cgcttttgtt cggcatgctg gtagaggttt
      481 tcgctctccg cggcaaagcc gacgcagaac gggccgtcgg gcaagcttgc aacatattca
      541 agaatgtccg gattgggcgc aagctgcaga ttcagtgttt gcccgccttt cttgattttt
      601 tgtgtgctgg gattgaccac gtgatagtcc gctaccgctg cgacactgat aaagatgtcg
      661 gcgtcgccgg ccgcctgcct gaccgctgca aacatttcct gcgcgctggt cacggatatg
      721 agtttgacgc gagcaggtgc ggccaggctc accgtaccgc ttaccaaagt cacttcagct
      781 cctgcttcca gcgcggcgca cgccgttgca tagcccatct tgccggaact caggttggta
      841 atgccgcgca ccgcatcaat cgcctcaaac gtggggcctg cggtaatcag cacatgcttg
      901 ccttgcagca gcctgggttg gaaactcgcg cggatatctt ccagcagcgt ttcgggctcg
      961 agcatacgac ccatccccgt ttcgccgcaa gcttgttcgc cgctctcagg cccgagtatc
     1021 gtgacgtcgt cttgttccag ttgcgcaata ttgcgctggg ttgcggggtt ttcccacatt
     1081 tgccggttca ttgccggcgc gaccagcagc gggcactcgc gcgccaggca cagcgtggag
     1141 agcaaatcat cggcgcgccc gtgcaccagc ttggcgatga agtcggcact cgccggcgca
     1201 accagcacaa cgtcggcttc gcggctcaaa tcaatatgcg ccatgttgtt ggcgatgcgc
     1261 gcatcccaca ggtcggtata gaccgtattg cccgagagcg cctggaacgt ggcggggccg
     1321 acaaaacggc aggccgcttc ggtcattact acttgcactt cgatgccgtc ctgcactaga
     1381 aggcgggtga gctccgccgc cttgtaggcg gcgatgccgc cggtgacgcc gagcagcagg
     1441 cgtttcgttt gggtcatgtt aaccatccgc aggctgttac gttactgtag agtgtaatat
     1501 attgatgcga attaagaaac cactaaagca atccaatcat gggaataagc cactggcctg
     1561 aaggcgagcg cccgagggaa aaactgttaa gcaagggcgc gcaaagcctg tcggaggcag
     1621 agctgctggc gatttttctg cgcacgggcg tggcgggaaa aagcgcggtg gatgtcgcgc
     1681 gcggtttaat caagcgcttc ggcagcctca cgcgcttgtt caacgcctcg cagaaggagc
     1741 tttgtgaagc accgggcctg gggccggcca agtacgcgca actgcgagcc gtgctggaaa
     1801 tggcgcggcg cacgcttgac gagaaaatca aagcaggcaa tgcgctcaat tcccccaagg
     1861 cggtgcgcga ctatctgctc cttgcgcttt ccgggcgcga gtatgaagtg tttgcgggga
     1921 ttttcctcga cgcgcaaaac cgcgtgctag agatggagga gttattccgc ggcactctca
     1981 gccagaccag tgtttttcct agggaggtgg tgaagcgcgc gctccatcac aacgcagcgg
     2041 gagtcatttt cgcgcataac catccctccg gcctggccga acccagcggc gctgatgagg
     2101 cgctcaccgc agctctcaaa aagtcgctcg cattggtgga tgtcaaagtg ctcgatcatt
     2161 ttattgttgg cagtggcggc atcatgtctt ttgccgagcg tggtttgttg tagagcacag
     2221 ctttcctggg tgcaaatctt ttccttgaga attaaccgtt aaatgggtaa aatgcgcact
     2281 cttccgaagt tgaggcaaag catcatggcc agagtgtgcg aagtaaccgg caagacgccg
     2341 atgttgggaa accgtgtttc ccacgccaac aacaagaccc ggcgccgttt tctccccaac
     2401 ttgcaatacc gcaagttctg ggtggaaagc gaaaagcgct gggtgcatct gcgcttgtcc
     2461 aacgccgcct tgcgcaccat tgataggaag ggcatcgaag cggtgctcgc cgagttgcgc
     2521 ggcaagaaat aaggaacggc gccatgcgcg aaaaaatcaa actcgaatca tccgcaggca
     2581 ccgggcattt ttataccacc accaagaaca agcgcaacca gacggataaa atggaaatca
     2641 agaaattcga cccggttgcc cgcaagcatg taatctacaa agaagttaaa ctcaaataag
     2701 ggtgcacctt gaaacaaaaa acccgccaag tggcgggttt tttgttgtcg taattcttta
     2761 tgcgtaacag cgcagcttgc gcgagaattc ctgcagcgaa gtgatgccgc ttttctcggc
     2821 gcggtggcac cagtcttcga gctgtcttac gagttgctcc ttgga
//
LOCUS       DATIOW010000079         6555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1252472, whole genome shotgun sequence.
ACCESSION   DATIOW010000079 DATIOW010000000
VERSION     DATIOW010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6555
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252472"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="VLL03_02460"
     CDS             complement(<1..1496)
                     /locus_tag="VLL03_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46260.1"
                     /translation="MALPEKAKTRPAQRQYSLLPAASLVLVSNLACAAEAGRIVAKDG
                     PALFGIPVDFILFALTLLGVALFHRHTLRVALTGLSVILGYKFLFVGFKYGPGFAGFV
                     SHMGHEWVILTNLFLLLVGFALLSRHFEESQMPAVLPKFLPDDWKGGFVLLVMIFVLS
                     SFLDNIAAAIIGGTVAATVFKHKVHIGYLAAIVAASNAGGSGSVVGDTTTTMMWIDGV
                     SPLDVFEAYVGASLAIVICGIPAALQQQRYSPIRKDPPRGVRIDWGRVAIVALILIAA
                     IVANIVVNLKFTDVSDRFPFIGAAVWLAILIAIPLRKPDWKVVPSAVKGSIFLLSLIL
                     CASLMPVEKLPAASWHTALGLGFVSAVFDNIPLTALALHQGGYDWGYLAYAVGFGGSM
                     IWFGSSAGVALSNMYPEAKSVGLWLKHGWHVTLAYVLGFAALLLVLGWHPDAKRVKTV
                     GFAPSVATGQRLNLVQNCRNKVMSQFECLLSAKSGHWPMFPGQSARVHR"
     gene            complement(1561..2688)
                     /locus_tag="VLL03_02465"
     CDS             complement(1561..2688)
                     /locus_tag="VLL03_02465"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HSS46261.1"
                     /translation="MYLDFKPSAALTLGVELELQLLKAHDCNLARDAADLLGRLKKVK
                     HSGEVKPEITESMIELNSSIHERYATLADELRVVRSVVTREAEVLNLRIAGGGSHPFH
                     QWSDRRIYPTERFKHLLNLYGYLAKQFTVFGQHIHIGCPSGDDAIYLIHMLSRHIPHF
                     IALSASSPFYQGEDTSFASSRLNAVSAFPLSGTMPLVQNWNEFNAYFDRMTRFKIVES
                     MKDFYWDIRPKPEYGTVEIRICDTPLTVDKAALLAAFAQALAHYYLSERPRAATPDLY
                     LVYNYNRFQACRYGFGANFINPYDESHSNLRDDLIVTLKFLAPHAEALESGAALNQLL
                     DEAAACRNDSSWLREQLKKTGSLNDVARLQSDLWMGKTSKA"
     gene            complement(2690..3910)
                     /locus_tag="VLL03_02470"
     CDS             complement(2690..3910)
                     /locus_tag="VLL03_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSS46262.1"
                     /translation="MPEFNFLPTLPLALTPPILFGLLLLAGLLGGEVARRIFFLPRIV
                     GYVLVGMLFGPIGLGLLSPRLIQQSQVFLDISLGLILFELGRRLDYPWLRRNRWLFTT
                     AIAESLFAFWAIYLGLRYFDYAPLFAAIAAAIGIATSPAVVLLVAHELRAEGQLTERM
                     LLLTAVNSVFAFFCATLLLPFLHLEHHAAWADTLLQPLYILLGSLLMGLAISFLVLNF
                     ASWLGKREDRQFIMLVSMIVLTVGLTRLLNLSVLVTLLTVGVLARNLDFRHKLMAVQF
                     GHGGQLFFVILFVLSGASLRFDALPQVGAVVAAYIMLRFLGKALAILLFARFSGLRPG
                     AAGLLGIALLPMSGLAIAMVQETSFLYPEFGTKLSAVVLSAVAILELVGPLATQFALK
                     QAGEARPASESTRI"
     gene            complement(4077..4718)
                     /locus_tag="VLL03_02475"
     CDS             complement(4077..4718)
                     /locus_tag="VLL03_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HSS46263.1"
                     /translation="MNARENILGRIRAALAKNERKTAREAMRAYIGQHAPGPRPQTEW
                     NLIARFRERALSLASSVDEIAALEQLPAASARYLREHNLPLQAVCWPEFAQLDWTAQS
                     MNVTARPANGNDLVGITGTFCAIAETGTLVMLSGKDTPSATSLLPETHVAVVRTSRIV
                     KGMEEAWALLRSELSTMPRAVNFISGPSRTADIEQTVTLGAHGPYRVHIILVN"
     gene            complement(4718..6226)
                     /locus_tag="VLL03_02480"
     CDS             complement(4718..6226)
                     /locus_tag="VLL03_02480"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HSS46264.1"
                     /translation="MNKPVETLTLLPLWIGGGKVASQSARWGDVTNPATGEVIRKAPL
                     CSAADIDAAVRAGKQAFPAWSETPPLKRARIMMRYLQLLEKNREQLARLASEEHGKTL
                     SDATGSVQRGIEVVEFACGIPHLLKGEHSENVGTGVDCYTLKQPLGVCAGITPFNFPV
                     MVPLWMFPVAIACGNTFVLKPSEKVPSASLRMAELFKEAGLPDGVFNVVHGDKEAVDA
                     ILHHPEIKAVSFVGSTPVAKYIYETCAKNGKRVQALGGAKNHAVVLPDADLEFTADAL
                     IGAAYGSAGERCMAISAVVAVGEAADPLIAKLRDKAAKLKVGPGNIKEVEMGPLVTRA
                     HRDKVKAYVDAGEKEGAKLVLDGRGVKVSGHENGFYLGPTLFDQVTPQMTIYRDEIFG
                     PVLIVLRALDYDEAIKLVNRNPYANGTAVFTRSGALARRFQHEIEVGMVGINVPIPVP
                     MAFFSFGGWKNSLFGDLHVHGPEGVKFYTRTKAVTGRWPEQTEKPKLDMPTH"
     gene            complement(6230..>6555)
                     /locus_tag="VLL03_02485"
     CDS             complement(6230..>6555)
                     /locus_tag="VLL03_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lactate utilization protein LutB
                     domain-containing protein"
                     /protein_id="HSS46265.1"
                     /translation="TFCGECEVVCPVKIPLPDLMRKLREKQVERGLRPWQERLALKVW
                     AWVAQRPGIYAAFTRIAARTLKGVAGEAKLLHHLPGGHGWTDGRDMPAPEGRTFRELY
                     QDRMR"
BASE COUNT         1470 a         2003 c         1874 g         1208 t
ORIGIN      
        1 cggtgtacgc gcgctgactg cccgggaaac attggccagt gtccgctttt ggccgaaagc
       61 agacactcaa actgagacat taccttattg cgacaattct ggactaaatt taacctctgg
      121 ccagtcgcaa cagacggtgc aaagccgacc gtctttacgc gcttggcatc cggatgccag
      181 ccgagcacca gcagcaaggc cgcaaaaccc aggacatatg ccaaggttac atgccaacca
      241 tgcttgagcc acaaaccgac cgacttggct tccgggtaca tgttcgacag cgccacgccg
      301 gccgaggagc cgaaccagat catcgaaccg ccgaaaccca ccgcatacgc aagataaccc
      361 cagtcgtatc cgccttgatg aagcgccagg gcggtgagcg gaatgttgtc gaacaccgcg
      421 gaaacaaaac ccaggcccag cgccgtgtgc caggaagcag cgggaagttt ttccacgggc
      481 atgagcgatg cacaaagaat cagcgacagc agaaaaatgc tgcccttgac cgcgctcggc
      541 accaccttcc agtccggctt gcgaagcgga atcgcaatca ggatcgccag ccacaccgca
      601 gcgccgatga aaggaaaacg atcggagaca tcggtaaact tgaggttgac cacgatgttg
      661 gcgacgatcg cggcaatcag gatcagcgcg acgatggcaa cccgccccca atcaatccgc
      721 accccccgcg gcgggtcctt cctgatcggc gaataacgct gctgctgcag cgccgccgga
      781 atgccgcata ttactatcgc cagcgacgcg cccacataag cttcaaacac gtcgagggga
      841 ctcactccgt caatccacat catcgtcgtg gtggtgtcgc cgaccacgct gcccgatcca
      901 ccggcgttcg aagcggcgac gatcgccgca aggtaaccga tatgcacttt gtgcttgaac
      961 accgtcgccg caaccgtgcc gccgatgatt gccgccgcga tgttgtcgag aaagctcgac
     1021 agcacaaaga tcatcaccag caacacgaac ccgcccttcc agtcgtcagg caggaatttc
     1081 ggcagcacgg ccggcatctg gctctcttcg aaatgcctgg aaagcagcgc gaatccgacc
     1141 agcagcagga acaggttggt gaggatcacc cactcatgcc ccatgtgaga aacaaaaccg
     1201 gcaaagccgg ggccgtactt gaacccgacg aacaggaact tataaccgag gatgacagag
     1261 aggccggtca gcgccacgcg cagggtgtgg cgatggaata gcgccacgcc caggagggtc
     1321 agcgcaaaca ggatgaaatc gacggggatc ccgaacaaag ccggcccgtc tttcgccaca
     1381 attctgccgg cttctgccgc gcaagcgaga ttgctcacca ggacaagaga tgccgcaggc
     1441 agcagcgaat attggcgttg cgccgggcgc gttttggctt tttcaggaag tgccatgccc
     1501 cctcccatgg acgcgcgatt ctagcatgcc ggcacgggga aacgcggctt gttccggttt
     1561 ttacgctttc gaggttttcc ccatccacaa atcgctttgc agccgcgcga cgtcattgag
     1621 cgaaccggtc tttttgagtt gttcgcgcaa ccaggatgaa tcattgcggc acgccgccgc
     1681 ctcgtcgagc aattggttga gcgccgcgcc gctttccagc gcttcggcat gcggggcgag
     1741 gaacttcagc gtcacaatca agtcgtcgcg caggttgctg tggctttcat cgtaaggatt
     1801 aatgaaattc gcgccgaaac catagcggca ggcctggaag cggttgtagt tgtaaaccag
     1861 ataaagatca ggggtcgccg cgcgcggccg ttcactcaag taataatgcg ccagcgcctg
     1921 ggcgaaggcc gcgagcagcg ccgccttgtc caccgtcaat ggcgtgtcgc aaatgcggat
     1981 ttccacggtc ccgtattccg gcttgggccg gatgtcccag tagaaatcct tcatgctctc
     2041 gacgattttg aagcgggtca tgcggtcgaa ataggcgttg aattcattcc agttttgcac
     2101 caacggcatg gtgccgctca agggaaaggc gctgacggcg ttgagacgtg aggaggcaaa
     2161 gctcgtatcc tcgccctggt aaaacggcga tgaggcggaa agcgcgatga aatgcggaat
     2221 gtggcgcgac agcatgtgga tgagatagat cgcgtcatcg ccgctcgggc agccgatgtg
     2281 gatatgctgg ccgaacacgg tgaactgctt ggcgagatag ccatagagat tcagcaaatg
     2341 cttgaagcgc tcggtcggat aaatgcgccg atcgctccac tggtgaaagg gatgcgagcc
     2401 gccgccggca atgcgcaggt tgaggacctc ggcttcgcgc gtcaccacac tgcgaacaac
     2461 ccgcaactcg tccgcaaggg tcgcgtagcg ctcgtggatc gaggaattga gctcgatcat
     2521 gctttcggtg atttccggct tgacctctcc ggaatgtttc acctttttca ggcgccccag
     2581 caaatcggcg gcgtcgcggg caagattgca atcgtgcgcc ttgagcagct ggagttcgag
     2641 ttcaacgccg agggtgagcg cggctgacgg tttaaaatcc aagtacatat taaattctgg
     2701 tgctttctga tgccggtctg gcctccccgg cctgcttcag ggcaaactgt gtggccaagg
     2761 ggccgacgag ctcaaggatc gccaccgccg acagtaccac cgccgacagc ttggtgccga
     2821 attcggggta gaggaatgag gtttcctgga ccatggcgat ggcgagcccc gacatcggca
     2881 gcaacgcgat gccgagcaag cccgccgcgc ccggacgcag gccgctaaaa cgggcgaaca
     2941 acagtatcgc caacgccttg ccgagaaagc gcagcatgat gtaggcggcg acaaccgccc
     3001 ccacctgcgg cagggcatca aaacgcaggc tggcgccgga caatacgaac agaatcacga
     3061 aaaacaattg acccccgtgg ccaaattgca ccgccatcag cttgtggcgg aaatccaggt
     3121 tgcgcgccag tactccgacg gtgagcagcg tcaccagcac cgaaagattg agtagacggg
     3181 tcaggcccac ggtgagcacg atcatgctca ccagcatgat gaattggcga tcctcgcgct
     3241 tgcccagcca gctggcgaaa ttcagcacca ggaagctaat cgccagaccc atcaacaacg
     3301 agcccagcaa aatgtaaagc ggctgcagca gcgtgtccgc ccaggccgca tggtgttcca
     3361 gatgcaggaa cggcagcagc agggtcgcgc aaaaaaaggc aaacacgctg ttgacggcgg
     3421 tgagcagcaa catgcgttcg gtgagctggc cttcggcgcg caattcgtgc gccaccagca
     3481 gcaccaccgc cggcgaagtg gcgatgccga tggcggcggc gatggcagca aacagcggcg
     3541 cgtaatcaaa atagcgcaag cccagataga ttgcccagaa cgcaaacagg ctttccgcaa
     3601 tcgcggtggt gaacagccag cggttacgcc gcagccacgg gtaatcaaga cggcggccga
     3661 gctcgaacag aatcaatccc agcgagatgt ccagaaacac ctggctttgc tgaatcagcc
     3721 gcgggctcaa taggcccaac cctatggggc cgaacagcat gccgaccagc acatagccga
     3781 cgatgcgcgg caggaaaaaa atgcgccgcg ccacttcgcc gccgagcagg cccgccagca
     3841 acagcaaacc gaacaggatg ggcggcgtga gcgcgagagg cagagttggc agaaagttga
     3901 actcgggcat gataattgga cagtgaagtg gaacaacgga tacggtgcgg gaacggttgt
     3961 ttcactcccg catgaggcag gaactgctat tttgctatct tgaagatagg cagattataa
     4021 ccagcagcag tttttcaggg caaccgaaat ttcgccggcc gtgccacgca aaaaaatcaa
     4081 ttgacgagga taatgtgcac tcgataaggg ccatgcgcgc caagagttac tgtctgttca
     4141 atatcagcgg tccttgaggg ccccgaaatg aaattgactg cgcgcggcat tgttgaaagt
     4201 tcgctccgca gcaaagccca ggcttcctcc atgcctttta caatccgtga agttctgacg
     4261 accgcaacat gcgtttcagg caaaaggctg gtcgcagagg gcgtatcttt tcccgaaagc
     4321 atgaccagcg ttccggtctc cgcaatcgcg cagaacgtgc cggtgatgcc taccagatca
     4381 tttccgttcg caggccgcgc ggttacattc atgctctgcg cagtccaatc gagctgcgca
     4441 aactccggcc agcacacggc ttgcaagggc aaattgtgtt cgcgcaaata acgtgcagac
     4501 gccgcgggca actgctcgag cgcagcgatt tcatccacgc tgctggcaag gctcaacgcc
     4561 cgctcgcgaa agcgcgcgat gagattccac tccgtttgcg gtctgggacc cggcgcgtgc
     4621 tgcccaatat aggcacgcat cgcttcgcgc gcggtttttc tttcgttttt ggcgagtgcg
     4681 gcgcggatac ggcctaagat gttttcacgt gcgttcatca gtgggtaggc atatcgagct
     4741 tgggtttttc cgtttgctcc ggccagcgcc ccgtaaccgc tttggtgcgg gtgtaaaact
     4801 tgacgccttc gggcccatgc acatgcaagt cgccgaacag cgaatttttc cagccgccga
     4861 aagagaaaaa tgccatcggc accggaatcg gcacgttgat gccgaccatg cccacttcga
     4921 tttcatgctg gaagcgccgc gccagtgcgc ccgagcgcgt gaacaccgcg gtgccattgg
     4981 catacgggtt gcggttgacc agtttaatcg cctcgtcgta atccagggcg cgcagcacga
     5041 tcagcaccgg gccgaaaatt tcatcgcggt aaatcgtcat ctgcggcgtg acctgatcaa
     5101 acagcgtggg ccccagataa aaaccgtttt catggccgga aaccttgaca ccgcgcccgt
     5161 ccagcaccag cttcgcgcct tctttttcgc cggcgtcaac ataagctttc accttgtcgc
     5221 ggtgggcgcg cgtcaccagc gggcccattt ccacttcctt gatgtttccc ggcccgacct
     5281 tgagttttgc ggccttgtcg cgcaatttcg caattaacgg atcggcggct tcgcccaccg
     5341 ccaccacggc ggaaatcgcc atgcagcgct cgcccgccga accgtaagcc gcgccgatca
     5401 aggcgtccgc ggtaaattcc agatcagcgt ccggcaacac cactgcatgg tttttcgcac
     5461 cgcccagcgc ctgcacccgc ttgccgttct tcgcgcaagt ttcataaatg tatttggcga
     5521 ccggcgtcga acccacaaag gaaaccgcct tgatttccgg atgatgcaaa atcgcatcca
     5581 ccgcttcctt gtcgccgtgc accacgttga acacgccgtc gggcaatccc gcttccttga
     5641 acagttccgc catgcgcagg ctcgccgaag gcactttttc agaaggttta agcacaaaag
     5701 tgttgccgca ggcaatcgcc accgggaaca tccaaagcgg caccatcacc gggaaattga
     5761 acggcgtgat tcccgcgcac acgccaagcg gctgtttgag cgtatagcaa tccacgccgg
     5821 tgcctacatt ttctgaatgc tcgcccttga gcaaatgcgg aatgccgcag gcgaattcca
     5881 cgacttcaat gccgcgctgc accgagccgg tggcgtccga caatgttttg ccgtgctctt
     5941 cacttgccag ccgcgccagt tgttcgcggt ttttttccaa aagctgcaaa taacgcatca
     6001 tgatgcgcgc acgcttgagc ggcggggttt cgctccaggc gggaaacgcc tgttttccag
     6061 cgcgcaccgc ggcatcaata tcggcggcgc tgcaaagcgg cgccttgcgg atgacttccc
     6121 cggtggcggg attggtcacg tcgccccagc gcgcgctttg gctcgcaact ttgccgccgc
     6181 cgatccacaa cggcagtaga gtaagggttt caacaggttt attcatggct cacctcattc
     6241 ggtcttggta cagctcgcgg aaggtacgac cttcaggcgc cggcatatcg cgcccgtcag
     6301 tccagccgtg ccctcccgga agatgatgca gaagttttgc ctcgcccgcc actcctttca
     6361 aagtgcgcgc cgcaatgcgc gtgaatgcgg cgtaaattcc aggccgctgc gcgacccatg
     6421 cccagacttt cagcgcgagc cgttcctgcc agggacgtaa cccccgctcc acctgctttt
     6481 ccctgagctt gcgcatcagg tcaggcaacg gaatcttcac cgggcacacc acctcgcact
     6541 cgccgcagaa cgtcg
//
LOCUS       DATIOW010000080        17165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1659960, whole genome shotgun sequence.
ACCESSION   DATIOW010000080 DATIOW010000000
VERSION     DATIOW010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17165
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659960"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VLL03_02490"
     CDS             <1..1050
                     /locus_tag="VLL03_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSS46266.1"
                     /translation="ICAAQKILPKSRQKRVISQHEIEGIVAKIARIPPQSVSTDDRAA
                     LKNLDRDLKAVVFGQDQAIDALSAAIKMARSGLGNPQKPIGCFLFSGPTGVGKTEVGR
                     QLAYIMGVELIRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEAITKHPYAVLLLD
                     EIEKAHPDIFNILLQVMDHGTLTDNNGRKADFRNVMIIMTTNAGAESLSKTAIGFTPG
                     KSSGDELVEIKRLFTPEFRNRLDAIISFAALDHEIILRVVDKFLMQLEEQLHEKKVEA
                     HFTDALKSYLAKRGFDPIMGARPMARLIQDTIRSALADELLFGRLAQGGKVTVDLDAN
                     EKMHLVFADEEEKVV"
     gene            1122..2930
                     /locus_tag="VLL03_02495"
     CDS             1122..2930
                     /locus_tag="VLL03_02495"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSS46267.1"
                     /translation="MSEATSAKLAGKPGTAAYPFIGLMATLVLAALVELISKSGLAVY
                     SPAPVLIFPVIFSAVQGGLRAGMASALLSALYFAYFYSLPGTVFHYDGKGLWYAVVWS
                     TATLAIVPLIAFLQQWRRKHLLDMPIERFQAYLAARKQTGQLLLSQEQFLQLIVERAL
                     DSMSVLDSRGVFQYGSPSIHAILGYVPGELLGKVGFDYIHPDDVDPAKQSFQEALETG
                     ILSQVDLRMRGKDGSWKVLEVTGRAVPRDSETLVVANIQDVTGRRRDEALIAAEKNLF
                     EMIAKDRPLAEILDALVRAIEEQTTGMLCSVLLLDADGVHLRHGAAPSLPDDYNKAVD
                     GLAIGPQAGSCGTAAFLKRLIVAEDTATDPRWAPYKDLASQYGLRASWSKPIMSATDK
                     VLGTFAVYYREPQSPRSRDLEIIGRVANLAAIAIERKQAQTQVEYMAHFDALTNLPNR
                     TLMLDRLKQALVQARRNKRGVALLFIDLDNFKEVNDSLGHAIGDAVLCAVAERLVKCV
                     RQGDTVSRQGGDEFVIILPNMQQREDARPVTQKILDAFATPFAVGGRDFVITASIGIS
                     FHPEHGEGAETLMKNADSAMYRAKELGKNKFHFYGT"
     gene            3053..3355
                     /locus_tag="VLL03_02500"
     CDS             3053..3355
                     /locus_tag="VLL03_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSS46268.1"
                     /translation="MKFHAAILLFTLGFGASLAAPAAGPNTARELAANCANCHGTDGK
                     SAGVMPPLAGLDRSYLVRHMQDFKAGRRPATIMHQLAKGYSDEQIELIADYFAAQK"
     gene            3364..4632
                     /locus_tag="VLL03_02505"
     CDS             3364..4632
                     /locus_tag="VLL03_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSS46269.1"
                     /translation="MVLSRREFIKWASAALGTAWFAGCASVRQGGGRVVVIGGGYAGA
                     TAAKYVRKWAPNLEVVLIERNTTFISCPLSNLVLGGNTTLCALTVGYDGLSKYGVKLI
                     NDEAVAVEAQQRKVQLARGASLSYDRLIVAPGIHFMYEQIHGLNNPEAQQKVLHAWKA
                     GTQTMALRNQLEAMPDGGVYALCIPKSPYRCPPGPYERACQVAFYFKQAKPHSKVLIL
                     DANDDVQSKKGLFTKAWKERYDGIVEYRPNSILVDVDAATLTARLEFENVSADVLNVI
                     PPQRAGNIAQAAGLVTANNRWCGVDFLTYESAAQKNIHVLGDAVVGAPAMPKSGHMAN
                     QQAKVCAAAVAALLNEQPVNSQPMMNNTCYSFVSGSEAMHVASVHRYDAAKKTMLVVE
                     GSTGLSTAPTELEGQYAWGWARNIWADMLT"
     gene            complement(4696..5823)
                     /locus_tag="VLL03_02510"
     CDS             complement(4696..5823)
                     /locus_tag="VLL03_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSS46270.1"
                     /translation="MSAGYRIDITDTLSAIAPQDWNRLAGNSPFQQHEFFSALHETGC
                     ASAQTGWAPRFILLWREERLLGAMPLYLKAHSYGEYVFDWAWADAYRHFGQHYYPKLL
                     CAVPFTPITGRRLLAETAEQRSLLLHAALNLARQSGASSFHCLFPEEPEAQELQSHGL
                     MLRQGLQFHWENRGYRDFADFLEALSRRKRKNIRQERRKVREAGITYEWLEGRNITDE
                     YWRFFTACYNHTYRQHFSTPYLNLDFFARLGETLSQYLLLILAKRESQPLAAALNLYT
                     PERLYGRYWGALEPIPGLHFETCYYQAIEFCIARGIRCFEGGAQGEHKLARGFLPVKT
                     WSAHWLAHPQFAQAIARHLDRETSGIAHYADELNEQSPFKK"
     gene            complement(5839..5991)
                     /locus_tag="VLL03_02515"
     CDS             complement(5839..5991)
                     /locus_tag="VLL03_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46271.1"
                     /translation="MQPMSPYPWYQLGMAHHHLNHSDKVAEIIAYLERFDPKMTRQLK
                     NDTRNT"
     gene            complement(5979..6398)
                     /locus_tag="VLL03_02520"
     CDS             complement(5979..6398)
                     /locus_tag="VLL03_02520"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS46272.1"
                     /translation="MQSIEHWLHFYVGRVLLMFNLQDAAAERLHLALKCNPRFDRAAS
                     SLGFIYASMRQYPVAARYFEQALQINPQAAGVWFNLGFIHEEQKHYSRAIEAFHKAVA
                     LNPKIDRAGYGMGLVHAALGEHEKPRRRFRRRPPCSR"
     gene            6456..8057
                     /locus_tag="VLL03_02525"
     CDS             6456..8057
                     /locus_tag="VLL03_02525"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HSS46273.1"
                     /translation="MKIAIAQINVTVGDLAGNAAKVLDSARQAKAGGASLVLAPELAL
                     SGYPPEDLLLRDGFYQACNKVLQDLAGQVQGITLVVGHPQAVGDRRFNAASVIRDGKI
                     IATYHKLKLPNYTVFDEERYFVSGSRPCVFELESVRFGVNICADIWEPHAAARAKDAG
                     AQVLLVLNASPYHMNKQYTRHEVLQERVRETGMAIIYGNLVGGQDELVFDGASFAVNS
                     EGELTHQFPPFQEGIYWVELEKHVPVRGQIAVAQSLEAMVYQALCLGVRDYVTKNGFP
                     GALVGLSGGIDSALTLAIAVDALGADKVRAVMMPSPYTASMSVEDARALAKALGVRYS
                     EIIIKSMFDAFLQALSSEFAGLPADTAEENLQARIRGTLLMALSNKTGGLVLTTGNKS
                     EMGVGYATLYGDMAGGFAVLKDITKTLVYRLAHYRNSLARVIPERILTRAPSAELKPN
                     QTDQDSLPVYDILDAIMEAYVEDDMNPEEIISRGFSADDVRRVVRLIKSSEYKRRQAP
                     VGIRITPRGFGKDWRYPITSKYRDI"
     gene            8141..8479
                     /locus_tag="VLL03_02530"
     CDS             8141..8479
                     /locus_tag="VLL03_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HSS46274.1"
                     /translation="MKKIEAVIKPFKLDEVREALSAIGVSGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKVELVVPEKLVESAIEAIIKAARTGKIGDGKIFVTSVEQVVRI
                     RTGETDEAAI"
     gene            complement(8546..9109)
                     /locus_tag="VLL03_02535"
     CDS             complement(8546..9109)
                     /locus_tag="VLL03_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HSS46275.1"
                     /translation="MNTRKGATHHPAPLPEEERGLFHEAVKGAQPLRAKRRVQHQPEP
                     PPPLPLQTLRDEQAALAESLTDWENPIETGDETSFLRAGLSRQILKKLRRGQWVIQSQ
                     LDLHGQNREEARSSLVAFLNTSIKRGLRCVRIIHGKGLGSKNRVPVLKHKVRGWLIKR
                     EEILAFCEAPPVDGGSGVLIVLLRSRG"
     gene            complement(9113..10078)
                     /gene="trxB"
                     /locus_tag="VLL03_02540"
     CDS             complement(9113..10078)
                     /gene="trxB"
                     /locus_tag="VLL03_02540"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HSS46276.1"
                     /translation="MSAASKPNKHSRLLILGSGPAGYTAAVYAARANLKPVLITGLAQ
                     GGQLMTTTDVDNWPADAMGVQGPELMERFQKHAERFDTEVIFDHIHTAQLSKKPLTLV
                     GDQGIYTCDALIIASGASAKYLGLPSEQAYLGKGVSGCATCDGFFYKGQEVAVVGGGN
                     TAVEEALYLSNIAKKVTVVHRRDKFRAEKILIDRLMGKTRNGNITVMWDHVADEVLGD
                     DSGVTGLRIKNAKTGALSRLDVKGVFIAIGHTPNTDLFKGQLEMENGYIVIKGGNQGN
                     ATATSISGVFAAGDVQDHVYRQAVTSAGSGCMAALDAEKYLDNLG"
     gene            10417..11100
                     /locus_tag="VLL03_02545"
     CDS             10417..11100
                     /locus_tag="VLL03_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HSS46277.1"
                     /translation="MSPSVISLLENVPLFSGLREEELATMVRVAVMKNYGKHAAILRE
                     GEKSDALYLVASGKVKVVLSTDDGKEVILAIIGKGEFFGEMALIDEQPRSADVVAMEP
                     SQLLVIGKSDFINCLAHNPQMAFRIMQGLIQRLRNADRKIESLALMDVYGRVARTLLD
                     LAKPENGKLVVGEKLSQQDLADMIGASREMVSRILRDLTVSGHIRVVAKKIILDQDLP
                     ETLTSASKM"
     gene            11361..13676
                     /locus_tag="VLL03_02550"
     CDS             11361..13676
                     /locus_tag="VLL03_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HSS46278.1"
                     /translation="MLSPYNRSKSSDERKPLPPRLAALLRESWWLMLIALALYVALVL
                     VSYDKLDPAWSHSANRAGDVHNLGGPVGAWLADILLYVFGFSAYWWVVFLLFATWWGY
                     QRIDTVSLSDRRSFFVAACGFALLLLASSGMEALRIHSIKVILPLAAGGMLGAILSKA
                     TVKALGLTGGTLILLMLIAVGVTLFTGFSWVKLMERLGGWIEDAYLFVINAWRNWKDQ
                     RRGRVAVTERDVFVEEEKKRFEEHEPIRIQPPVAEIPKSARVEREKQAPLFENLPDTP
                     LPPLHLLDQADESVEVLSADTLEFTSRLIEKKLLDFGVEVKVVAAYPGPVITRYEIEP
                     AVGVKGSQIVNLAKDLARALSVVSIRVVETIPGKACMGLEIPNPERQVVRLSEILSSQ
                     AYADLSSPLTLALGKDIGGNPVVADLQRMPHLLVAGTTGSGKSVAINAMILSLVYKSP
                     PQQVRLILVDPKMLELSVYEGIPHLLAPVVTDMKRAANALNWCVEEMDRRLRLMSSMG
                     VRHLAGYNQKIRDAQKVGKLLADPFSVTPENPEPLEQLPLIVVVIDELADIMMVVGKK
                     VEELIARLAQKARAAGVHLLLATQRPSVDVITGLIKANIPSRIAFQVSAKVDSRTILD
                     QVGAEALIGQGDMLYLPPGLGYPQRVHGAFVADHEVHKVVGYLKSLGAPQYVNEVLES
                     AETEAGGEGLPADAETDPLYDEAVAIVLKSHRASISLVQRHLRIGYNRAARLIEQMER
                     AGLVSGMQNNGIREVLAPAGDIDYPPRKPMY"
     gene            13701..14279
                     /locus_tag="VLL03_02555"
     CDS             13701..14279
                     /locus_tag="VLL03_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HSS46279.1"
                     /translation="MNKLLCFTLCALLSGAAAGEVYKWVDDDGVTHYSENAPPDKAAR
                     QVDVRPFGVETDDDKGCHTIPCQAERLQKQKPAESPRVQVPDLVRGLPFDIFIHIERG
                     MTEAEVLIRTGKPDQEVIEGTEEHSAAAASAANSVNPQTGVVTRNANIIKNTITQVVK
                     TYYYLPTISDPFTTILTFKGGRVADIQRIRKL"
     gene            14282..14899
                     /gene="lolA"
                     /locus_tag="VLL03_02560"
     CDS             14282..14899
                     /gene="lolA"
                     /locus_tag="VLL03_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HSS46280.1"
                     /translation="MKAGCSLFLLLALGGLVPAHASSIDKLKSFIQQSSSARAQFSQQ
                     LLDKNGRSVQQASGVMQFSRPGKFRWAYEKPYAQLIVGDGEKLWVYDPELNQVTVKKL
                     DQGLGSSPAALLAGNNEIENSFALKDAGTKDGLDWLEALPKDKDGAFERVRMGFGRLD
                     LEVMELRDQFGQTTVLRFSSLERNPRLNPDLFKFTPPKDADVLGD"
     gene            14892..16202
                     /locus_tag="VLL03_02565"
     CDS             14892..16202
                     /locus_tag="VLL03_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HSS46281.1"
                     /translation="MTDLFQRKAPQAPLAERLRPQKLDDVIGQAHLLGVGKPLRLAFE
                     SGKPHSMILWGPPGSGKTTLARLMAQAFNAEFIAHSAVLSGVKEIRDAVSRAELVLQQ
                     SGRHTILFIDEVHRFNKAQQDAFLPFVERGLVTFVGATTENPSFEVNSALLSRAAVYV
                     LHPLSDEELGVLFERAKKNILPLAVFADEAKLLLVGYADGDARRLLNMLEQLATAAQQ
                     DQRTDIGAEYVQNVLAQTVRRFDKGGDAFYDQISALHKSVRGSNPDAALYWMCRMLDG
                     GADPRYIGRRLVRMATEDIGLADPRALRLALDACEVYERLGTPEGELALAEAAVYLAC
                     TAKSNAVYMAYNDARRFIREDKSRPVPEHLRNAPTKLMKEMGFGKEYRYAHDEPEAYA
                     AGENYFPEDMPAQNFYQPTERGLEAKITERLKYLHELDGKAKKK"
     gene            16214..>17165
                     /gene="serS"
                     /locus_tag="VLL03_02570"
     CDS             16214..>17165
                     /gene="serS"
                     /locus_tag="VLL03_02570"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HSS46282.1"
                     /translation="MLDIQLLRSDLDRIAKRIEARGCEFNTAEFKELEAERKVIQSKT
                     ESYQSQRNARSKEIGQAKAKGDEKLVTQILAEVAEFGDQLKRMEIELVSIQSRLQKFL
                     LDIPNVPHESVPFGKSPEDNAEVRRVGSPRKFDFPVRDHVDLGEGLKQLDFAAASKIS
                     GARFALMKGDLARLHRAIAQFMLDVHSNEHGYTEIYAPYLVNADSMRGTGQLPKFETD
                     LFAVQKSENEKLYLIPTAEVPLTNIVRDEILPLETLPLKFVCHSPCFRSEAGNYGKDT
                     RGMIRQHQFDKVELVQIVHPAKSYVALEELTADAETILKKL"
BASE COUNT         3635 a         4800 c         5178 g         3552 t
ORIGIN      
        1 atctgcgcgg cgcagaaaat cctgcctaaa tcaaggcaaa aacgcgtcat cagccagcac
       61 gaaatcgagg gcatcgtcgc caagatcgcg cgcatcccgc cgcagagcgt gtccaccgac
      121 gaccgcgccg cgctgaagaa tctcgaccgc gatttgaaag cggtagtgtt tggccaggat
      181 caggcaattg acgcgctttc cgccgccatc aagatggcga ggagcggcct gggcaatccg
      241 caaaaaccta tcggttgctt cctgttctcg gggcctaccg gtgtgggcaa gaccgaagtc
      301 ggacgccagc tcgcctacat catgggggtg gaactgatac gtttcgacat gtcggaatac
      361 atggagcggc atgccgtgtc gcgcctgatc ggcgcgccgc ccggctatgt gggttttgat
      421 caagggggct tactcaccga agccatcacc aagcatcctt acgcggtctt gctgctcgac
      481 gagatcgaga aggcgcaccc tgacatcttt aatatcctgt tgcaggtgat ggaccacggc
      541 acgctcaccg acaacaacgg ccgcaaggcg gatttccgca acgtgatgat catcatgacc
      601 accaacgccg gcgccgagtc gctgtcgaaa accgccattg gcttcacccc cggcaagtcc
      661 tccggtgacg aactggtgga gatcaagcgg ctgttcaccc cggaattccg caaccggctg
      721 gacgccatta tttccttcgc cgcgctcgac cacgaaataa tcctgcgtgt ggtagacaag
      781 tttctgatgc agctcgagga gcagttgcac gagaaaaaag tcgaggcgca tttcaccgat
      841 gcgttgaaaa gctatctggc gaagcgcggc ttcgatccaa taatgggtgc gcgccccatg
      901 gccaggttga tccaggacac catccgcagt gcgctggcgg atgaactcct gtttggcagg
      961 ctcgcccagg gcggcaaggt caccgttgac cttgatgcta acgaaaaaat gcatctggtg
     1021 tttgcagacg aagaagaaaa agtcgtttag ctctttaccc cttgcgcccg gccgcagaat
     1081 tggcggactc gggctgcaag agcgactaag cccgggaggc catgtccgaa gccacatctg
     1141 ctaagctggc cgggaagccc ggcaccgctg catatccgtt cattggcctg atggccactc
     1201 tggtgctggc ggcgctggtg gagcttattt ccaaaagcgg tttggctgtt tatagcccgg
     1261 ctcccgtgct gattttcccg gtaatatttt ccgccgtgca aggcggcttg cgcgcgggca
     1321 tggccagcgc tctgctgtcc gccttgtact ttgcgtattt ctattcgctt ccgggtacgg
     1381 ttttccatta cgacggcaaa ggcttgtggt atgccgtggt ctggagcacg gccacgctcg
     1441 ccatcgtgcc gctcattgcc ttcctgcagc agtggcgccg caaacacctg ttagatatgc
     1501 ccatcgagcg gtttcaagcg tatctcgcgg cacgcaagca aacaggacag ctgctcctca
     1561 gtcaggagca atttctgcaa ctgattgtgg agcgggcgct cgattcgatg tcggtgctgg
     1621 acagccgcgg cgtgtttcaa tacggcagcc cctccatcca tgccattctc ggttacgtgc
     1681 cgggagaact gctgggtaaa gtcggcttcg actacatcca tcctgacgac gttgaccccg
     1741 ccaaacagtc tttccaagag gcgctggaga caggtatcct cagtcaggtt gatctacgca
     1801 tgcgcggcaa ggacggcagt tggaaagtgc tggaagtgac gggccgtgcg gtaccgcggg
     1861 atagcgagac tctggtcgtc gccaatatcc aggatgtcac cggtcgccgg cgcgacgaag
     1921 cgctgattgc cgctgagaaa aatctgtttg aaatgattgc caaagaccgg ccgctggcgg
     1981 aaatcctcga cgcattggtg cgtgccatag aagagcagac cacgggcatg ttgtgttcgg
     2041 tgctccttct cgacgcggac ggcgtgcatt tgcgtcatgg tgcggcgccg agcctcccgg
     2101 atgattacaa caaggccgtc gatggtttgg ccattgggcc gcaggcaggc tcctgcggta
     2161 cggcggcttt cttaaagagg ttgattgtgg ccgaggacac agccaccgac ccgcgatggg
     2221 caccgtacaa agacctcgcg tcgcagtacg ggttgcgcgc ctcctggtcc aagcccatca
     2281 tgtctgccac cgacaaggtg ctcggcacgt ttgccgtgta ctaccgcgag ccgcaaagcc
     2341 cgcggtcccg cgacctggaa atcattggcc gcgtggcgaa tctcgccgcc atcgccatcg
     2401 agcgcaagca ggcacaaact caagtggagt acatggcgca tttcgatgcg ctgactaatt
     2461 tgcccaaccg cacactgatg ctggatcgtc ttaagcaggc cctggtgcag gcgcggcgca
     2521 ataagcgcgg cgtggcgctt ttgttcattg acctcgacaa cttcaaggag gtgaacgaca
     2581 gcctgggcca cgccatcggc gatgcagtat tgtgcgccgt agccgagcgc cttgtgaagt
     2641 gcgtgcgtca gggtgatacc gtgtcccggc agggcgggga tgaattcgtt atcatccttc
     2701 ccaatatgca gcagcgggaa gacgccaggc cggtgacgca gaaaatcctt gatgcgtttg
     2761 ccaccccgtt tgcggtgggc ggccgcgatt ttgtcattac cgcgagcatc ggcatcagct
     2821 tccaccctga acacggcgag ggtgcggaaa ccctgatgaa aaatgctgac agcgccatgt
     2881 accgcgccaa ggaactgggc aaaaacaagt ttcactttta cggcacctag tcttgccacg
     2941 gccggtactt gacacggtcg ggcagcggag taaaactttc cagtaggcgt ctgacccgtt
     3001 tgcgccagcg caggagcgtt ctgcaatccg cgatgactcg ataggagaag cgatgaaatt
     3061 ccatgcggcg atcctgttat tcactttggg tttcggcgca agcctggctg ccccggccgc
     3121 tggcccgaat actgcgcgtg agcttgccgc caactgtgcc aactgtcacg gcaccgacgg
     3181 taaaagcgct ggcgtaatgc cgcccctcgc agggctcgat cgcagctatc tcgttcgaca
     3241 catgcaggat ttcaaagcag gccgccgccc ggccaccatc atgcatcaac tggcgaaggg
     3301 ctatagcgat gagcagatcg aactcatcgc cgattatttc gcagcacaaa aatagggggc
     3361 gatatggtcc tttcgcggcg tgaattcatc aaatgggcat ccgccgcgct cggcacggcc
     3421 tggtttgcgg gttgtgcgag cgtgagacaa ggcggcggtc gcgtggtggt gatcggcggc
     3481 ggctatgcgg gcgccaccgc ggcaaaatat gtgagaaaat gggcacccaa tctggaagta
     3541 gtcctgatcg aacgcaacac cacgttcatt tcctgcccgc tttccaactt ggtgctgggc
     3601 ggcaatacga ccctgtgtgc gcttacggtc ggctacgacg ggttgagcaa gtacggcgtg
     3661 aaactcatca acgacgaagc ggtggcggta gaggcgcagc agcgcaaggt gcaactcgcg
     3721 agaggcgcga gcttgagtta cgaccggctg attgtcgcgc ccggcatcca cttcatgtac
     3781 gagcaaattc acggcctcaa taatccggaa gcacagcaaa aggtgctgca tgcctggaaa
     3841 gcgggcacgc aaaccatggc gctgcgaaat caactggaag cgatgccgga cggcggggtt
     3901 tatgcgctgt gcattcccaa gtcaccgtac cgttgcccgc cgggacctta tgagcgcgcc
     3961 tgccaggtgg cgttttattt caagcaggct aagccgcatt ccaaggtgct catcctcgac
     4021 gccaatgacg atgtgcagtc gaagaagggc ttgtttacca aggcctggaa ggagcgttac
     4081 gacggcatcg tcgaataccg gccgaacagc atcctggttg acgtagacgc ggcgacgctc
     4141 accgccagac tggaattcga gaacgtcagt gccgacgtgt tgaacgtgat tccgccgcag
     4201 cgcgccggga acatcgcgca ggccgcgggg cttgtcaccg ccaacaaccg ctggtgcgga
     4261 gtggattttc tgacctatga atcggctgcg cagaaaaaca tccatgtgct gggcgatgcg
     4321 gttgttggcg ctccggccat gcccaagtcc gggcacatgg cgaaccagca ggcgaaagtc
     4381 tgcgccgcgg cggtggcggc gctgctaaac gaacagccgg tcaattccca gccgatgatg
     4441 aacaatacct gttacagttt tgtcagcggc agcgaagcca tgcatgttgc ctcggtgcac
     4501 cgttatgatg cggcgaagaa aaccatgctg gtggtcgaag gcagcacggg gctatctacc
     4561 gcgcctactg agctggaagg tcagtacgcc tgggggtggg ccaggaatat ctgggcggac
     4621 atgctcacct gaaaaacggc gtcttttggg ccaatactgt gttgccttgt ttttctcagt
     4681 cctttacgtg caacctcatt tcttaaacgg gctctgctcg ttgagttcat cggcgtagtg
     4741 cgcgattcct gaagtttccc gatcgagatg gcgcgcaatc gcctgcgcga attgcggatg
     4801 cgcgagccag tgcgccgacc aggtcttgac cggcagaaag ccgcgcgcga gcttgtgctc
     4861 gccttgcgcg ccgccttcga agcagcgaat cccccgcgcg atgcaaaact caatcgcttg
     4921 gtaatagcag gtctcgaagt gcaatccggg aataggttcc agcgcgcccc aatagcgccc
     4981 gtataaacgt tcaggcgtgt aaaggttgag tgcggcggcc agcggttgac tttcgcgctt
     5041 ggccagaatc agcagcaagt actgggacag cgtttctccc agccgtgcga aaaagtccag
     5101 gttgaggtag ggtgttgaaa aatgctggcg gtaagtatgg ttgtaacagg cggtgaaaaa
     5161 acgccaatac tcatcagtga tgttcctccc ctccaaccat tcgtaagtaa tacccgcttc
     5221 ccgcactttg cgtcgttctt gacggatgtt cttgcgcttg cggcggctca aggcctcaag
     5281 gaaatcggca aagtcgcggt agccccggtt ttcccagtga aactgcaagc cctgtctcag
     5341 cattaagccg tgcgattgca gttcctgtgc ttccggctct tccggaaaca ggcagtggaa
     5401 cgatgaggct ccggattgtc tcgccaaatt cagcgcggca tgcaacagca acgagcgctg
     5461 ttcagccgtt tcggccagca ggcgtctgcc ggtgatggga gtgaaaggaa cggcgcagag
     5521 cagcttggga taatagtgct ggccgaaatg ccggtaggcg tcggcccatg cccagtcgaa
     5581 cacgtattcg ccgtatgaat gcgccttgag ataaagcggc atggcgccga gcaaccgttc
     5641 ttcgcgccaa aggaggatga agcgcggggc ccagccggtt tgcgccgagg cgcagccggt
     5701 ttcgtgcaat gcgctgaaaa attcgtgttg ctggaaaggg ctgttgccag ccaggcggtt
     5761 ccagtcttgg ggcgcaatgg cggaaagtgt gtcggttata tcaatgcgat atccggcgct
     5821 catggctttt cagccgactt aggtgttgcg ggtgtcgttc ttgagctgcc gcgtcatttt
     5881 aggatcgaat ctttccagat acgcgatgat ttcggcgacc ttgtcggagt ggttcaggtg
     5941 gtgatgcgcc atgcccagtt gataccaggg gtaggggctc atcggctgca aggtggccgc
     6001 ctcctgaaac gcctccgcgg cttttcatgt tcgccgagcg cggcgtgaac aaggcccatg
     6061 ccgtacccgg cgcggtcgat cttggggttg agcgccaccg ctttgtggaa cgcctcgata
     6121 gcccgggagt agtgtttctg ctcttcgtga atgaaaccga ggttgaacca gacgccggca
     6181 gcctgcggat tgatttgcaa cgcctgctcg aaataacgtg cggccaccgg atattgccgc
     6241 atggacgcat agatgaaccc cagggaactc gcggcgcgat caaaccgcgg gttgcacttc
     6301 agcgccaggt gcaggcgctc cgccgccgcg tcctgcaggt taaacatgag cagaacgcgt
     6361 ccgacataaa aatgcagcca gtgttcaatc gattgcatcg gttttttgct tgggcttgga
     6421 ttatgggaag gaaagcaaaa tgttatattg tcactatgaa aatagctatt gcccagatca
     6481 acgttaccgt cggcgatctt gccggcaacg ccgccaaagt cctggactct gccaggcagg
     6541 cgaaagccgg cggtgcgagc ctggtgctgg cgccggaact ggccctctcc ggctatccgc
     6601 cggaagacct gttgttgcgc gacgggtttt accaggcctg caacaaggtt cttcaggacc
     6661 tggcgggcca ggttcaaggc atcacgctcg tagtggggca tccgcaggcg gtcggcgatc
     6721 ggcgctttaa cgccgcgtcg gtgattcgcg acggcaaaat catcgccact taccacaagc
     6781 tcaagcttcc caattacaca gtgtttgacg aggaacgtta tttcgtgtca ggaagccggc
     6841 cttgcgtgtt cgagctcgag agcgtgcgct tcggcgtgaa catttgcgcc gacatctggg
     6901 agccccatgc ggcggcccgg gcgaaagacg ccggcgcgca ggtgctgctg gtgctgaacg
     6961 cctcgccgta tcacatgaac aaacagtata cccgccacga ggtattgcag gagcgggtca
     7021 gggaaaccgg tatggcgatc atttatggca atctggtcgg cggccaggat gaactggtgt
     7081 tcgacggcgc ttcgtttgcg gtgaacagcg aaggcgagct cacgcatcag tttccgccgt
     7141 ttcaagaagg tatttattgg gtcgagctgg aaaagcacgt gccggtgcgc ggccagatcg
     7201 ccgtcgccca atcgctcgaa gcgatggtgt atcaggcatt gtgcctgggc gtgcgcgatt
     7261 atgtgacgaa gaacggcttc cccggcgcgc tggtcggcct ttccggcggc atcgactcgg
     7321 cgctgacgct cgccattgcg gtggacgcgc tgggtgcaga caaggttcgg gcggtgatga
     7381 tgccttcgcc ctacaccgcc agcatgagcg tcgaggatgc gcgcgccctc gccaaagcat
     7441 tgggcgtgcg ttacagcgaa attatcatca agtccatgtt cgatgcattt ttgcaggcgc
     7501 tctcctctga attcgccggc ttgcctgccg atactgccga ggaaaatttg caggcgcgca
     7561 ttcgcggcac gttgctcatg gcgctgtcca acaaaactgg cggcctggtt ttgaccaccg
     7621 gcaataagag cgaaatggga gtagggtatg ccacgcttta cggcgacatg gcgggcggct
     7681 tcgcggtgtt gaaagacatc accaaaacac tggtgtaccg cctggcgcat taccgcaaca
     7741 gcctggcgcg cgtcattccc gagcgcatcc ttacccgtgc gccgtcggcg gagctgaaac
     7801 cgaaccagac cgaccaggac agcctgccgg tgtacgacat tctggacgcc attatggaag
     7861 cttatgtcga ggatgacatg aacccggagg aaatcatcag ccgcggcttc agtgctgacg
     7921 atgtgcggcg cgtggtgcgc ttgattaaaa gcagcgaata caagcggcgc caggcgccgg
     7981 tgggcatccg catcactccc cgtggcttcg gcaaggactg gcgttatccg attacctcga
     8041 aataccgaga catctgattt gctgttgccg tagcggcatt tatctctttc gcatttggca
     8101 ctatacttac gtctgattgg tatccccacg ggagaaactg atgaaaaaaa tagaagccgt
     8161 tatcaaaccc ttcaagctcg acgaagtgcg ggaagccctg tccgccatcg gcgtcagcgg
     8221 tcttaccgtg accgaagtca agggctttgg ccggcagaag ggccacaccg agctttatcg
     8281 cggcgcggaa tacgtggtgg atttcctgcc caaggtgaaa gtggaactgg tggtgccgga
     8341 aaaactcgtg gagtccgcaa tcgaggctat catcaaggcc gctcgcaccg gcaagatcgg
     8401 cgacggcaag atttttgtca catccgtgga acaggtggtg cgcattcgca ccggcgaaac
     8461 tgacgaagcg gcgatttgaa aagcagtgac gagtgacaag tgacttgtca ctcgttactt
     8521 gttactcgtc actcgtccct aatccctatc ccctgcttct cagcagcaca attagcacgc
     8581 cgctgccgcc gtcaaccggg ggcgcctcgc agaacgccag aatctcttcg cgcttaatta
     8641 gccacccccg taccttgtgc ttcagcaccg gcacgcggtt tttcgagccc aggcccttgc
     8701 catgaataat acgcacacag cgcaaaccgc gcttgatact tgtattaaga aacgccacga
     8761 gacttgaacg cgcttcttcg cggttttgtc cgtgcagatc gagctgactc tgaatcaccc
     8821 actggcctcg gcgcagtttt ttcaggattt gcctggataa gccggcacgc aggaaagacg
     8881 tttcatcgcc ggtttcaatc gggttttccc aatcagtgag cgattcggcg agcgccgcct
     8941 gttcatcgcg cagggtttgc aagggcagag gcggaggcgg ctcgggttga tgctgcacgc
     9001 gccgcttggc gcgcaagggc tgggcgcctt ttaccgcttc atggaacaag ccgcgttctt
     9061 cttccggcaa aggtgctgga tggtgtgtcg cgcctttacg ggtgttcatt ctttatccga
     9121 gattgtcgag atatttttcc gcatccagcg cagccatgca gccggagcct gcgctcgtca
     9181 ctgcctggcg ataaacatgg tcctgcacat cgcccgccgc gaacacgccc gaaatgctgg
     9241 tagcggtggc attgccctga ttgccgcctt tgatgacgat gtaaccattc tccatttcca
     9301 gctggccctt gaaaagatcg gtgttcggcg tgtggccgat ggcaataaac acgcctttca
     9361 catcaagccg gctcaaagcc ccggtcttgg cattcttgat gcgcagtccg gtgacgccgc
     9421 tgtcgtcgcc aagaacttca tccgccacat ggtcccacat gactgttatg ttgccattgc
     9481 gggtttttcc catcagccgg tcaatcagga ttttttcggc gcggaacttg tcgcggcggt
     9541 gcactaccgt gacttttttg gcgatgttgg agaggtagag cgcttcctcc acggcggtgt
     9601 tgccgccgcc caccaccgca acctcctgcc ctttgtaaaa gaaaccgtca caagtcgcgc
     9661 aaccggacac gcctttgccc agatacgcct gttccgaggg caggccgaga tatttggccg
     9721 atgcgccgga ggcaataata agcgcatcgc aggtgtagat cccctgatcg ccgaccagcg
     9781 tcaatggctt tttggacagt tgcgccgtgt gaatgtgatc gaaaatgacc tcggtatcga
     9841 atcgctccgc atgtttctgg aagcgctcca tcaactccgg accctgcacg cccatggcat
     9901 ccgccggcca gttgtcgaca tcggtggtgg tcatgagttg cccgccttgc gccagcccgg
     9961 taatcaacac cggtttcaag ttggctcgcg cggcatagac ggcggcggta taaccagcgg
    10021 ggccggagcc caaaatcagc aggcggctat gtttgttggg tttgctcgcg gcggacatgg
    10081 ttgattcggg agttgggcga agtttgaggc aatgtaaatg agattattgg atatgaggcc
    10141 attagaccgg ttttcaagcg gtaaatgtct taaggcgaaa ttttaataac tgtgcgaatt
    10201 tatcagtaat agacaaccct gtcgagttcc tcgagagaag cgtgaatttt cgagcgtata
    10261 ggtctataat taagcatcga aaaacttcat tcaatcttgg gaatggtgga tgaaccgctt
    10321 aacaaaaatg atgctcgcct ttgaggggtc tgtcgctctc atgacccgcg ttgcccagga
    10381 ggtaaaagct ccgccgtgtt ccggcgcatc aaactcatga gcccctctgt catcagcttg
    10441 ctggaaaacg tgcccctgtt ctccgggctg cgggaggaag aactggcgac aatggtgcgc
    10501 gttgccgtga tgaaaaacta cggcaagcat gccgccatcc tgcgtgaggg cgaaaaaagc
    10561 gacgcgctct accttgttgc ttccggcaag gtcaaggtgg ttttgagcac ggacgacggc
    10621 aaggaagtga tcctggcgat tatcggcaag ggtgaattct tcggtgaaat ggcgctgatc
    10681 gatgagcagc cgcgctcggc cgacgtggtt gccatggaac ccagccagct cctcgtcatc
    10741 ggcaagtccg atttcatcaa ttgcctggcc cacaacccgc aaatggcgtt tcgcatcatg
    10801 caaggcctga tccagcggct gcgcaacgcc gaccgcaaga tcgaaagcct cgcgctcatg
    10861 gatgtctacg gcagagtggc acggacccta cttgacctgg ccaagccgga gaacggcaaa
    10921 ttggtggtcg gggaaaaact ttcgcaacaa gaccttgccg acatgatagg cgcctcgcgc
    10981 gaaatggtga gccgaatttt gcgcgatttg accgtttccg ggcatattcg cgtcgtagcc
    11041 aaaaaaatca ttctcgatca ggatttgccg gagactttaa ccagcgcttc caagatgtga
    11101 cgccggtaat tgtcgtgggt tatacttcaa acagaggctg actgtaatct acgagtgagg
    11161 gggcctcggc ttttgatccg gcgggccggg caacttaaag agcgagtggc acgccagatc
    11221 aaaccgttac caggcatctc cggaaacagc ccctgtttgt gccaagcagg ggctgtttca
    11281 cgtgagaaaa ccggaaacag gaacccgtgt gccccaactt gatataatcg gcaaaacgga
    11341 ttaagtggtc cggcctttcg atgctttccc cttataacag atccaaatca tctgacgagc
    11401 ggaaaccgct cccgcccagg cttgcggcgc tgctgcgcga atcctggtgg cttatgctga
    11461 tagcgttggc gctttatgtg gcgctggtgc tggtgagcta tgacaagctg gaccccgcct
    11521 ggtcgcattc cgccaaccgt gccggagacg tgcacaatct cggcggcccc gtgggcgcct
    11581 ggcttgccga tattctgctc tatgtcttcg gcttttccgc ctactggtgg gtggtgttcc
    11641 tgttgtttgc gacttggtgg gggtaccagc gcattgatac ggtaagcctc agcgaccgcc
    11701 gttccttctt tgtggccgca tgcggtttcg ccttgctgct gctcgccagc agcggaatgg
    11761 aagcgctgcg cattcacagc atcaaggtca tcctgccgct ggccgcgggc ggcatgctgg
    11821 gcgcaattct cagcaaggca acagtcaaag ccctgggcct taccggcggc accttgattc
    11881 tgctgatgct tatcgccgtg ggcgtgaccc tcttcaccgg gttttcctgg gtaaaactca
    11941 tggagcggct gggcggctgg attgaagacg cttatctttt tgtcatcaac gcctggcgca
    12001 attggaagga ccagcgccgg ggccgggtag cggtgaccga gcgcgatgta ttcgtcgagg
    12061 aagaaaaaaa gcgcttcgag gaacacgagc cgatccgcat tcagccgccg gtcgcggaaa
    12121 tccccaagtc ggcgcgggtc gagcgagaaa aacaggcgcc gctgtttgaa aatttgcccg
    12181 acaccccgct gccgccgctg catctgctcg accaggccga tgagagtgtc gaagtgctgt
    12241 ccgccgacac gctggaattt acttctcgcc tgatcgagaa aaagctgctg gatttcggcg
    12301 tggaggtcaa ggtggtggcg gcctatcccg gcccggtgat tacgcgctat gaaatcgagc
    12361 cggcggtggg ggtaaaaggc agccagatcg tcaatctggc gaaagacctc gcgcgcgcgc
    12421 tgtcggtggt gagcatccgc gtggtcgaaa ccattcccgg caaggcctgc atggggctgg
    12481 aaattcccaa tccggaacgg caggtagtga ggctctccga aatcctgagc tcgcaggcct
    12541 acgccgacct gtcttcgccg ctcacgctgg cgctcggcaa agacatcggc ggcaatccgg
    12601 tggtggcgga tctgcagaga atgccgcatt tgctggtggc gggtaccacc ggttccggca
    12661 agtcggtggc gatcaacgcg atgattctga gcctggtgta caagtccccg ccgcagcagg
    12721 tgcggttgat tctggttgac ccgaagatgc tggaactttc ggtctatgaa ggcatcccac
    12781 atttgttggc gccggtggtg accgacatga agcgggcggc gaacgcgctc aactggtgcg
    12841 tggaggaaat ggaccggcgg ttgcggctga tgtcaagcat gggcgtgcgt catctcgccg
    12901 gttataacca gaaaatccgc gatgcgcaaa aggtcggcaa gctgcttgcc gacccattca
    12961 gcgtcacgcc ggaaaatccg gagccgctgg aacagctgcc gctcatcgtg gtggtgattg
    13021 acgagcttgc cgacatcatg atggtggtgg gcaagaaagt ggaagaattg attgcgcgcc
    13081 tggcacaaaa ggcgcgcgcg gcgggggtgc atttgttgct ggcgacgcaa cggccgtcgg
    13141 tagacgtcat taccggcctc atcaaggcca acatcccgag ccgcattgcg ttccaggtgt
    13201 cggcgaaagt ggattcgcgc accatactcg accaagtggg cgcagaggcg ctcatcggcc
    13261 agggcgacat gttgtatctg ccgcccggat tgggttaccc gcagcgcgta cacggcgcgt
    13321 ttgtcgccga ccacgaagtg cataaggtgg taggctatct gaagagtttg ggggcgccgc
    13381 agtatgtgaa tgaggtgctg gaaagcgcgg agaccgaagc gggcggcgag ggattgcccg
    13441 ccgatgcgga aaccgacccg ctgtatgacg aggcggtcgc catcgtgctg aaatcgcacc
    13501 gcgcttcgat ttcgctggtg cagcgccatt tgcgcatcgg ctacaaccgc gccgcgcgcc
    13561 tgattgagca aatggagcgc gccggtttgg tctcgggtat gcagaacaac ggcatccgcg
    13621 aagtgctggc gccggcgggt gacatagatt atccgccccg caagccaatg tactgaactg
    13681 tgcgccgagg aagggtctcg atgaacaagt tgctttgttt tactctgtgt gccctgttga
    13741 gtggcgcagc ggcgggcgag gtttataagt gggtcgacga tgacggcgtg acgcattatt
    13801 cggaaaatgc gccccccgat aaagcggcgc ggcaggttga tgtgcgtccg ttcggtgttg
    13861 agaccgacga cgacaaaggt tgtcacacca ttccttgcca ggcggagagg ctgcaaaaac
    13921 agaagccggc cgagtcccct cgtgtgcagg ttccggatct ggtgagggga ctgccttttg
    13981 acatcttcat tcatattgaa aggggaatga ccgaggcgga agtcctgatc cggaccggca
    14041 agcccgacca agaggtcatc gaaggtaccg aagagcatag cgccgccgcg gcttccgctg
    14101 ccaacagtgt caacccgcaa accggtgttg tcaccaggaa cgccaatatc atcaagaata
    14161 caatcaccca agtcgtcaaa acttattatt atctgcccac catatccgat ccctttacca
    14221 ccatcctcac cttcaagggc ggccgggtgg cggatattca aagaatccgc aaactctgaa
    14281 catgaaggcc gggtgttccc tctttttgct gcttgcgctg ggcggcctgg ttccggcgca
    14341 cgcgtccagc atcgacaagc tgaaaagctt tatccagcaa tcgtcctcgg cgcgagcgca
    14401 gttcagccag cagttgctgg acaagaacgg ccgcagcgtg cagcaggcaa gcggcgtcat
    14461 gcaattctcc cgcccgggca agttccgctg ggcgtatgaa aaaccctatg cgcaacttat
    14521 cgtcggcgat ggtgaaaaac tctgggtgta cgatcctgag ctcaatcagg tcacggtaaa
    14581 aaaacttgac caggggctgg gctcaagccc cgctgcgctg ctcgccggca acaatgaaat
    14641 cgaaaatagt ttcgcgttga aagatgccgg cacgaaggat ggcctggatt ggcttgaggc
    14701 gttgccaaaa gacaaagatg gcgcctttga gcgcgtgcgt atggggtttg gccgcctgga
    14761 cctggaagtg atggaattgc gcgaccagtt cgggcagacc acggtattgc gtttttccag
    14821 ccttgaacgc aatccaaggc tcaaccctga tcttttcaaa ttcacaccgc ccaaagacgc
    14881 ggacgtcctc ggtgactgat ttattccaaa ggaaagcgcc gcaagcgccg ctcgctgagc
    14941 gcctgcggcc gcagaaactg gatgacgtca tcggccaggc gcatctgctc ggggtgggca
    15001 aacctttgcg gctcgccttc gaatccggca agccgcactc gatgatactg tggggtccgc
    15061 cgggatcggg caaaaccacg ctggcgcggc tcatggcaca ggcgttcaat gccgagttca
    15121 tcgcgcattc cgccgtgctt tccggcgtca aggaaatacg cgacgcggtg agccgggcgg
    15181 aattggtgtt gcaacaatcg gggcgccaca cgattctctt cattgatgaa gtgcaccgct
    15241 tcaacaaggc gcagcaggat gcgtttttgc ctttcgttga gcgggggttg gtgactttcg
    15301 tcggcgccac cacggaaaac ccttcatttg aagtcaacag cgcgctgctt tcgcgcgctg
    15361 ccgtgtatgt gctgcacccg ctttccgatg aggagctcgg tgttttgttt gaacgcgcaa
    15421 agaagaatat tctacccctt gccgtttttg ccgacgaggc gaaattgctc ttggtgggtt
    15481 acgccgacgg cgatgcgcgg cgtctgctta acatgctgga gcagctggcc accgctgcgc
    15541 agcaggacca gcgcaccgac atcggcgcgg aatacgtgca aaacgtgctg gcgcaaaccg
    15601 tcaggcgttt tgacaaaggg ggcgacgcgt tttatgacca aatttccgcc ctgcataaat
    15661 cagtgcgcgg ctctaatccc gacgcggcgc tctattggat gtgccgcatg ctcgatggcg
    15721 gcgccgaccc gcgctacatc ggcaggcggc tggtgcgcat ggcgacggaa gacatagggc
    15781 ttgccgaccc gcgcgcgctc agactggcgc tggacgcctg cgaagtgtac gaacgtctgg
    15841 gcacgcccga aggagagctc gcgctcgccg aagcggcggt ttatctcgcc tgcactgcca
    15901 agagcaacgc cgtgtacatg gcgtataatg acgcgcgccg ctttatccgc gaagataaat
    15961 cgcgcccggt gccggagcat ttacgcaacg cgccgaccaa gctgatgaag gaaatgggtt
    16021 tcggcaaaga gtatcgttac gcacacgatg aaccggaagc ctatgcggcg ggagaaaatt
    16081 attttcccga agacatgccc gcgcagaatt tttatcagcc gacggagcgc gggctggagg
    16141 cgaaaattac agagcgcctg aagtacttgc atgagctcga cggaaaagcg aagaaaaaat
    16201 aacagggaaa accatgctcg atattcagtt attgcgcagc gacctagata ggatcgctaa
    16261 aagaattgaa gcacgggggt gcgagtttaa taccgccgaa ttcaaagagc ttgaggccga
    16321 gagaaaagtt atccaaagta aaaccgagtc ctaccagtcg cagcgtaacg ctcgctcgaa
    16381 agaaattggc caagctaagg cgaagggtga cgagaaactg gttacccaaa tcctggcgga
    16441 agtagcagag tttggcgatc agctcaaacg gatggagatt gagcttgttt ccattcagtc
    16501 aagactgcaa aagttccttc tggacatccc aaatgttccg cacgagagcg tgcctttcgg
    16561 caaatcgccc gaagataatg ctgaagtacg gagagtaggc agcccgcgca aattcgattt
    16621 cccggtcagg gatcatgtgg atttgggcga ggggctcaaa cagctcgatt ttgccgcagc
    16681 gagcaaaatc agtggcgcgc gctttgcctt aatgaagggc gacctggcgc gcctgcaccg
    16741 cgcaattgcc cagttcatgc tcgatgtgca cagcaatgag cacggttaca ccgagatcta
    16801 tgcgccgtat ctggtgaacg cagattccat gcgtggcacc ggccagttgc cgaagttcga
    16861 gaccgatttg ttcgcggtgc agaaaagtga gaatgaaaag ctctatctga ttcccactgc
    16921 tgaagtaccg ctcaccaata tcgtgcgcga tgaaatcctg ccgctggaaa cattgccgct
    16981 caaattcgtt tgccactcgc cgtgtttccg ctcggaagcc ggcaactatg gcaaggatac
    17041 gcgcggcatg atacgccagc atcaattcga caaggtggag ctggtgcaaa tcgtgcatcc
    17101 cgccaaatcc tacgtggcgc tggaggaatt gaccgctgat gctgagacca tattgaaaaa
    17161 actgg
//
LOCUS       DATIOW010000081         4526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1660191, whole genome shotgun sequence.
ACCESSION   DATIOW010000081 DATIOW010000000
VERSION     DATIOW010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660191"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /gene="pcnB"
                     /locus_tag="VLL03_02575"
     CDS             complement(<1..1279)
                     /gene="pcnB"
                     /locus_tag="VLL03_02575"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HSS46283.1"
                     /translation="MISKLLRRVFSKQTSPSGAPLVIPRKQHGITRSQISPGALKTVA
                     ALRDKGYTAFVVGGAVRDLLLGLKPKDFDVATDATPEQVRTVFRRAHIIGRRFRLVHV
                     MHGAETVEVSTFRGTQSEGDGAEQDEHGRILRDNVFGTRQQDAERRDFTVNALFYDPQ
                     NEEVLDYVGGFADLQARRLRIIGDAVSRYREDPMRMLRAVRFAAKLGFKIEPSTHAPI
                     LKLATLLQNVPASRLFEEMLKLLLSGHAAASVKALREHGLHQGLLPMLDLIIEPPLEQ
                     RFFAAALKNTDDRIHENKPVSPGFLFAALLWHEVLAAWKNFQSRGVQPAPALHQAMEQ
                     VISQQAQRLAIPRRFTATMKEIWTSQPRFLQRAGKRPFRLVEHPRFRAGYDFLLLRCD
                     SGEVDAEIGKWWTAFQNAGQEQRRNMLLIDQTPG"
     gene            complement(1329..1994)
                     /locus_tag="VLL03_02580"
     CDS             complement(1329..1994)
                     /locus_tag="VLL03_02580"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSS46284.1"
                     /translation="MRLALFDLDNTLLAGDSDFEWAQFLIDKQVLDHEVYEAQNRDFY
                     EQYQAGTLDIHAFLDFQLMPLSRHPKSELETWRAEFMRKRIVPIVNKKARLLVQSRLE
                     DGDLTAIITATNRFVTAPIAVEFGVPHLIATEPEEKNGKFTGKVTGIPCFREGKVACL
                     ENWLKEQGMQFNSFAESWFFSDSSNDLPLLQRVTHPVTVDPDDRLRAHAEKHHWQIIS
                     LRQ"
     gene            complement(2122..2814)
                     /gene="hda"
                     /locus_tag="VLL03_02585"
     CDS             complement(2122..2814)
                     /gene="hda"
                     /locus_tag="VLL03_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HSS46285.1"
                     /translation="MQSQMALDLSLRSEPSLDNFVAGKNAELIQVLREMLAKPRERFL
                     YLWGGPGSGRSHLLKAMVDAFRQQHLSASYISCAADIEFAPGVDQLDMIAVDDVERLS
                     SPVQIDLFNLYNHVREGQAVLLVSGKLPPAQLRLREDLITRLGWGLVFQVHALNDEEK
                     AQALKQHARERGFALPQDVADYLLRHGRRDMPSLLAILDALDNFSLQAKRPVTLPLLR
                     ELIQSSLFPGGQ"
     gene            complement(2879..3937)
                     /locus_tag="VLL03_02590"
     CDS             complement(2879..3937)
                     /locus_tag="VLL03_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSS46286.1"
                     /translation="MNTKRFDDVYWWLGLAVIAAVLIYLLSPILTPFLLAAVLAYICN
                     PLVDTLQRRKIPRTLSVVLAMLLLLGLTALLVLILLPVLRQQLVTLAERLPAYLDTIK
                     SGILPWLHARFGLELQLDATSLAAALTENLPAAKDLAVKLLPSLKSGGLALIAFLMNL
                     LLVPVVLFYFLRDWNGLIKRIQEIIPRGLHAEITGLAAETDRVLGEFLRGQISVMLLM
                     SAYYAVALWLAGLDYALPIAVLAGFLVFVPYLGMIVGLTLATLAGFLQFPDFIGLIPV
                     WVAFAIGQVLEGMVVTPWLVGERVGLHPVAVIFALLAFGQLFGFFGVLLALPASSALL
                     VWLRHLRRRYMESAIYKS"
     gene            4003..>4526
                     /gene="purM"
                     /locus_tag="VLL03_02595"
     CDS             4003..>4526
                     /gene="purM"
                     /locus_tag="VLL03_02595"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HSS46287.1"
                     /translation="MNQSSSLSYRGAGVDIDAGDALVENIKPFARKTLRPEVLMGIGG
                     FGALVKISKKYRDPVLVSGTDGVGTKLKLAFALNRHDTVGIDLVAMSVNDILVQGAEP
                     LFFLDYFACGKLEVATATQVIKGIAKGCGLAGCALIGGETAEMPGFYPDGEYDLAGFI
                     VGVVEREKIITGKTV"
BASE COUNT          992 a         1359 c         1303 g          872 t
ORIGIN      
        1 tccctggcgt ttggtcaatc agcagcatgt tgcggcgctg ttcttggccg gcgttttgaa
       61 acgccgtcca ccattttccg atttcagcgt ccacttcacc gctgtcacag cgcagcagca
      121 gaaaatcgta gccggcgcga aagcggggat gttcaaccaa gcggaacggc cgcttgccgg
      181 cgcgctgcaa aaaacgcggc tgcgacgtcc agatttcctt catcgtcgcg gtaaagcgcc
      241 gcgggatcgc cagtcgctgc gcctgctggc ttatcacctg ctccatcgcc tgatgcagcg
      301 cgggcgcggg ttgcacgccc cggctttgga agtttttcca tgccgccagc acttcatgcc
      361 acaacagcgc ggcgaacaga aaaccgggtg agaccggctt gttctcgtgg atgcggtcat
      421 cggtattctt gagcgcggcc gcgaaaaagc gctgctccag cggcggctcg atgatcaggt
      481 ccagcatcgg cagcaaaccc tggtgtagtc catgttcgcg cagtgctttc acgctggccg
      541 cagcatggcc ggagagcagg agcttcaaca tttcctcgaa caggcgcgag gcgggaacgt
      601 tttgcaaaag cgtcgcgagc ttgaggatag gcgcgtgtgt ggacggctcg attttgaagc
      661 cgagcttcgc ggcaaagcgc accgcgcgca acatgcgcat cgggtcttcg cggtagcggc
      721 tgacggcatc gccgatgatg cgcagccggc gcgcctgcaa atcggcaaag ccgcccacat
      781 agtcgagaac ttcctcgttc tgaggatcgt aaaacaaggc gttgacggtg aaatcgcggc
      841 gctccgcatc ctgctgccgg gtgccaaaca cattgtcgcg caggatgcgc ccatgctcat
      901 cctgctccgc gccatcgccc tctgactgcg tcccgcgaaa agtggaaact tccaccgttt
      961 ccgcgccatg catcacatgc accagccgga agcggcggcc gatgatgtgg gcgcgccgga
     1021 acaccgtgcg tacctgctcg ggtgtggcat cggtcgccac atcgaaatcc ttgggcttca
     1081 atccgagcag caaatcccgc accgccccgc ccaccacaaa cgccgtgtag cccttgtcgc
     1141 gcaatgcggc gacggttttc agcgcgcctg ggctgatctg gctgcgagtg atgccatgct
     1201 gcttgcgcgg gatgacgagc ggcgcgccgg aaggggatgt ttgcttgctg aaaacgcggc
     1261 gcagaagttt gctgatcatg atgagcaaat tgaattatac actgcggccg cccccgagct
     1321 ttcggcggtt actggcgcag actgatgatc tgccagtggt gcttttcggc atgcgccctc
     1381 aagcggtcgt ccggatccac cgtgaccgga tgcgtcacgc gctgcaacag gggcaaatca
     1441 ttcgaggaat cgctgaaaaa ccaactttca gcaaatgagt tgaattgcat tccctgctct
     1501 ttcagccagt tctccaggca ggcgactttc ccttcgcgaa aacagggaat gcccgtcacc
     1561 ttgccggtaa acttgccatt cttctcttcc ggctcggtgg cgatgaggtg ggggacgcca
     1621 aattccacgg cgatgggcgc cgtcacgaag cggttggtgg cggtgatgat ggcggtcaaa
     1681 tcgccgtctt ccaggcggct ttgcaccagc agccgcgctt ttttgttcac gatgggcact
     1741 atccgctttc gcatgaactc ggcgcgccag gtttccagct cgctcttcgg atggcgcgac
     1801 aagggcatca actggaaatc gagaaaagca tggatgtcga gcgtgccggc ttggtattgt
     1861 tcgtagaaat cccggttttg cgcttcgtac acctcatggt ccagcacttg tttgtcgatc
     1921 aggaattgcg cccattcaaa atcgctgtcg ccggcgagga gtgtgttatc gaggtcaaac
     1981 aaagcaagtc gcacggctta cctatctggc ttgtaaataa tgttatcccc tttccaactc
     2041 tcccccgcta gcgggggaga ggcgtttgtc ccctccccac ccatgtgggg agggtttggg
     2101 tggggtaagg gttggccagt ctcactgccc ccccggaaac aaggatgatt ggatgagttc
     2161 gcgcagcagc ggcaaagtca ccggccgctt ggcctgcagg gaaaaattat ccagcgcatc
     2221 gagtatggcg agcagtgaag gcatgtcgcg ccggccgtga cgcagcagat aatcggccac
     2281 atcctgcggc agcgcaaaac cgcgctcccg ggcatgttgt ttcagggcct gcgctttttc
     2341 ctcgtcgtta agcgcgtgca cctggaacac caatccccag ccgagccgcg taatcaaatc
     2401 ctcgcgcagc ctgagttgcg ccggcggcag tttgccgctt accaaaagca ccgcctggcc
     2461 ctcgcgcacg tggttgtaaa gattaaagag gtcgatttgt acaggggacg acagccgctc
     2521 gacatcatca accgcgatca tgtcgagctg gtccaccccc ggtgcaaatt cgatgtcggc
     2581 ggcgcaggaa atgtaagacg cgctcaaatg ctgctggcgg aatgcatcaa ccatggcttt
     2641 cagcaaatgg ctgcggccgg aaccgggacc tccccagaga taaaggaagc gctccctggg
     2701 tttagccagc atctcgcgca gcacctgtat cagctccgcg ttcttgccgg cgacaaaatt
     2761 atccagcgag ggctcgctgc gcagggataa atccagcgcc atttggcttt gcatgattaa
     2821 gctcagtttg caaattcggg ccaaccacag cagtatatcg cccttagtcc ggccacagtc
     2881 aggatttgta aatcgcgctt tccatgtagc ggcgccgcag atggcgcaac caaaccagca
     2941 gcgccgagct tgccggcagc gccaacagca cgccgaaaaa accgaacagt tgcccgaagg
     3001 cgagcagcgc gaaaatcacc gccaccggat gcagccccac ccgctcgccc accagccagg
     3061 gcgtgaccac catgccttcc agcacctgac cgatggcgaa cgccacccac accggaatca
     3121 acccgatgaa atccggaaat tgcagaaacc ccgccagcgt ggccaaagtc aagcccacga
     3181 tcattccgag ataaggcacg aacaccaaaa acccggcgag cacggcgata gggagcgcgt
     3241 aatcgagacc cgccagccac agcgccaccg cgtaataggc gctcatcaac aacatcactg
     3301 aaatctggcc gcgcaaaaat tcccccagca cgcggtcggt ttccgcggcc aatcccgtga
     3361 tttctgcatg cagaccgcgc ggaataattt cttgtattct tttgataagc ccattccagt
     3421 cgcgcaggaa ataaaacagc accaccggca ccagcagcag attcatcaga aaggcgatga
     3481 gggcgagccc cccgcttttg agcgagggca aaagtttgac cgccaggtcc ttggccgccg
     3541 gcaggttttc ggtcaacgcc gccgccaagc tggttgcatc aagctgcaac tccagtccga
     3601 agcgggcatg cagccagggc agaatgcccg attttatcgt gtcgagataa gccggcaatc
     3661 gctctgccag cgtaaccagc tgctggcgca gcacgggcag cagaatcagc accagcagcg
     3721 cagttaatcc caataacaac agcatggcga gaaccacgct cagagtgcgc gggattttcc
     3781 ggcgttgcag ggtgtccacc agcggattgc aaatgtaggc gagaacagcc gccagcaaaa
     3841 aaggcgtgag tatcgggctc aacagataaa tcaacacggc ggcaatgact gccagcccga
     3901 gccaccaata tacatcgtcg aagcgtttgg tattcatttc aggtaaaatt caaggtgcaa
     3961 aaagccctgc cactttatct tgaacaacgc cataactcaa ccttgaacca aagctcctca
     4021 ctttcctacc gtggcgccgg cgtggacatc gatgccggcg acgccttggt ggagaacatc
     4081 aagccgtttg ccagaaaaac cctgcggccg gaagtgctga tggggatcgg cggttttggc
     4141 gcgctggtaa aaatttcaaa aaaataccgc gatccggtgc tggtttccgg caccgacggc
     4201 gtcggcacca agctcaagct cgctttcgcg cttaaccgac acgataccgt aggcattgac
     4261 ctggtggcga tgagcgtgaa cgatattctg gtgcagggcg cggagccgct gttttttctc
     4321 gattacttcg cctgcggcaa gctcgaagtg gcaaccgcga cgcaagtcat taaaggaatt
     4381 gccaaaggct gcgggcttgc cggctgcgcg ctgattggcg gcgaaaccgc ggaaatgccc
     4441 ggattctatc ccgacggtga gtacgacctg gcgggattta tcgtcggtgt ggtcgagcgc
     4501 gagaagatta ttaccggaaa aactgt
//
LOCUS       DATIOW010000082         2906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_34049, whole genome shotgun sequence.
ACCESSION   DATIOW010000082 DATIOW010000000
VERSION     DATIOW010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34049"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VLL03_02600"
     CDS             <1..233
                     /locus_tag="VLL03_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HSS46288.1"
                     /translation="RPVDDLELTVRSANCLKAENIYYIGDLIQRSETELLKTPNLGRK
                     SLNEIKEVLASRGLTLGMKLENWPPAGLENKA"
     gene            237..659
                     /gene="rplQ"
                     /locus_tag="VLL03_02605"
     CDS             237..659
                     /gene="rplQ"
                     /locus_tag="VLL03_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HSS46289.1"
                     /translation="MRHRQGLRKLNRTSSHRLAMLRNMTNSLLRFETIKTTLPKAKEL
                     RRVAEPLITLGKKPTLANRRLAYTRLRDRDMVAKLFNELGPRYAKRNGGYLRILKNGF
                     RVGDSAPMALVQLMDQPEPEAGSKTGTKAEPKAAKAKA"
     gene            822..1550
                     /locus_tag="VLL03_02610"
     CDS             822..1550
                     /locus_tag="VLL03_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HSS46290.1"
                     /translation="MIILFTDFGARDLYAGQVKAVLHHHAPGVRQIDLLHDAPAFNVK
                     TSAHLLAALSQPFPKGSVFLAVVDPSVGTAREAVAMTADGKWFVGPDNGLLSVLAARA
                     EKPRFWHISWRPQNLSHSFHGRDLFAPIAALVAQGVLPLDKMEEINSLRVQFGAPDLA
                     EIIYIDHYGNAMSGLRAKLLAPDGSLLVNGARLKRARVFAEVPSGTPFWYENCLGLAE
                     IAVNSSSAAVRLDLEIGDKIELVK"
     gene            complement(1678..2313)
                     /locus_tag="VLL03_02615"
     CDS             complement(1678..2313)
                     /locus_tag="VLL03_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019446943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HSS46291.1"
                     /translation="MRHYALFATLFAIAALAGCGSSPKERFYTLSSGAALERSYTKAP
                     YSIAIGAVTVPEVVNRPQLVTYIAPNQVKIAEQARWAQPLKSEIQRVIADNLTQLLDG
                     AYVSVYPENVSLNADYQVRVDVQRFDSALGDAVTIEALWTVRPVQGGAPKNGRSVVRE
                     PTNGNGYDALVAAHGRALAAVSRDIAEAIRAANASSQSSGLPDHEPSPTMH"
     gene            complement(2313..>2906)
                     /locus_tag="VLL03_02620"
     CDS             complement(2313..>2906)
                     /locus_tag="VLL03_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mammalian cell entry protein"
                     /protein_id="HSS46292.1"
                     /translation="RIYPGRLHALERMQTETLDRKELLDAWVKRGLRAQLRTGSLLTG
                     QLYIALDFFPHAPNVEVDWSKKPPRLPTLPGGLLELQTTITALADKLTVIANKLEKVP
                     YEQIGNDLSQTLQNANKLIQKLDTEVAPEARSVFIDARKTLAEAERLLSADAPLQQDT
                     REAMREIAHAAQALRVLADYLERHPETLIRGKTEDEK"
BASE COUNT          591 a          840 c          857 g          618 t
ORIGIN      
        1 tgcgcccggt ggacgatctg gagcttaccg tgcgttccgc gaactgcctg aaggcggaaa
       61 acatttatta catcggcgac ctgattcagc gcagcgaaac cgagttgctg aagaccccca
      121 atctcggccg caagtcgctc aacgaaatca aggaagtcct ggcttcgcgc gggctcacgc
      181 tcggcatgaa gcttgaaaac tggccgccgg cggggctgga aaacaaggca taaatcatgc
      241 gccaccgtca aggactgaga aagctcaacc gcacctccag ccaccggctg gcgatgctgc
      301 gcaacatgac caattcgctg ttgcgcttcg agaccatcaa aaccaccctg cccaaggcca
      361 aggaactgcg ccgcgtggcc gagccgctga ttacgctggg caagaaaccg acgctcgcca
      421 accggcgcct cgcctacact cgtctgcgcg accgtgatat ggtggcgaag ctgtttaacg
      481 agttgggccc gcgctacgcc aagcgcaacg gcggctatct gcgcatcctc aaaaacggct
      541 tccgcgtggg cgacagcgcc ccgatggccc tggtgcagct catggaccag ccggaacccg
      601 aggccgggtc caagacaggg accaaggccg agcccaaggc cgcaaaagcc aaagcctaag
      661 cggctggttt ggactccatt taaaaagccc ggcttgcggg cttttttatt tacttttacc
      721 gcggagacgc ggagacgcag aaagaattct cgaagattct aaaattcatt aaaacctctg
      781 tgcctctgcg tctctgtggt gaaaaggttt aaaattactt catgattatt ctgtttaccg
      841 atttcggcgc acgcgacctc tatgcggggc aggtcaaagc ggtgctccac caccatgcgc
      901 ccggcgtgcg gcagattgat ttgctgcacg acgcgccggc gttcaacgtc aaaaccagcg
      961 cgcacctgct ggcggctttg tcgcagccct tcccgaaggg aagcgtgttt cttgccgtgg
     1021 ttgatcccag cgtgggaacc gcgcgcgaag cggtggcgat gacggccgac ggcaaatggt
     1081 ttgtcgggcc cgacaacggc ctgctttccg tgctggcggc aagggcggag aaaccccgtt
     1141 tctggcacat ctcctggcgg ccgcagaatc tttcccattc ttttcacgga cgcgatttgt
     1201 ttgcgccgat cgcggcgctg gttgcccaag gcgtgcttcc gctcgacaaa atggaagaaa
     1261 ttaactcgct gcgagtacaa ttcggcgcac cggatctggc agaaatcatt tacatagacc
     1321 attacggcaa tgccatgagc ggcttgcgcg cgaaattact tgcgcctgat ggttcactgc
     1381 tggtgaacgg ggcccgcttg aagcgcgcca gggtgtttgc agaggttccg agcgggacgc
     1441 cgttttggta tgagaactgc cttggtctcg cggagatcgc agtgaactcc agtagtgcgg
     1501 cggtacggct cgacttggaa atcggtgaca aaattgaatt ggtgaaatga ccgtgatgat
     1561 tcgccgtggg cgactgcttc gcgcagcgat acctgattaa tcatccattc agcctaccga
     1621 gaaaacacga tttgatgccg gcccgcctga tttttttgta ctgggttgac aacagagtta
     1681 atgcattgtt ggtgagggtt catgatcggg gagcccgctt gactgagacg aagcgttggc
     1741 ggcgcggata gcctccgcga tgtcgcggct gactgccgcc agcgcccgtc catgggccgc
     1801 caccagcgcg tcgtagccat tgccgttcgt cggctcacgc actacactcc gcccattttt
     1861 cggcgcaccg ccttggacag gccgtaccgt ccacagggct tcgattgtca ctgcatcgcc
     1921 aagcgccgag tcgaagcgtt gcacgtccac ccgcacctga taatcggcat tgaggctcac
     1981 gttctccgga tagacggaga catacgcacc atccagcagt tgcgtaaggt tatccgcgat
     2041 cacacgctgg atttcactct tcagcggctg agcccagcgc gcctgttcgg cgatcttcac
     2101 ctggttgggg gcgatgtaag tcacaagctg aggacggttg acaacctcag gcacggtcac
     2161 cgctccaatt gcgatgctgt atggggcctt ggtgtacgaa cgttcgagtg ctgcgccgga
     2221 gctcagggta taaaaacgct ccttgggcga gctgccgcag ccggcaagcg ccgcgattgc
     2281 gaacagggtt gcaaagagtg cgtaatggcg catcactttt catcctccgt tttgccgcgt
     2341 atcagagttt cgggatggcg ttcgagataa tcggcgagca cgcgtagcgc ttgggctgcg
     2401 tgtgcgattt cgcgcattgc ctcgcgcgtg tcctgttgca gcggcgcgtc agcagacaac
     2461 aagcgttccg cctctgcaag tgttttgcgc gcatcgataa acacgctgcg cgcttcaggc
     2521 gcaacctccg tgtcgagttt ctggatcagc ttgttcgcat tttgcagcgt ctggctcaga
     2581 tcgttgccga tctgttcgta cggcactttc tccagcttgt tcgcgatcac agtaagttta
     2641 tccgcgagcg cagtaatggt tgtttgcagt tccagcaggc cgccgggtaa agtcggtaat
     2701 cgaggcggtt tcttggacca atcgacctcg acattgggtg catgggggaa gaaatcgagc
     2761 gcgatgtaga gctggcccgt cagtaaactt ccggtacgca actgtgcgcg gagaccgcgt
     2821 tttacccatg cgtccaaaag ttccttgcga tcgagggttt cggtctgcat ccgctcaagc
     2881 gcgtgcaaac gcccaggata gatccg
//
LOCUS       DATIOW010000083         4039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1761711, whole genome shotgun sequence.
ACCESSION   DATIOW010000083 DATIOW010000000
VERSION     DATIOW010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1761711"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VLL03_02625"
     CDS             complement(<1..422)
                     /locus_tag="VLL03_02625"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HSS46293.1"
                     /translation="MDLSKALEVAMATDPGMVRTHNEDSIAADAAGGFVILADGMGGY
                     NAGEVASGIATAMLGATLGQALQARDPGQIEPESGEPMAVKMLKEHIAGANTSIYHAA
                     KSQPLYAGMGTTLVAGLFYDNRVSVAHLGDSRLYRLRG"
     gene            complement(433..1206)
                     /locus_tag="VLL03_02630"
     CDS             complement(433..1206)
                     /locus_tag="VLL03_02630"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HSS46294.1"
                     /translation="MKKLAFWKADWFLGLVVAIVLYFAGGSDLIQSLERKAYDLGVQA
                     SSRSPSGKVAVIAIDDQSIANIGRWPWSREVHAKMIDLLASVKAKVIGNTVFFSEPQV
                     VPGLVYISNLNQVLANSGIKNNTDPLVQQDVSQIETLLKEAEQNLNSDLKLSESIAKA
                     NNVLLALLFQLSEPRGKPDQPLPEFVEKNRLSNVEDKIAAVSDGLLPDATTAALIPLA
                     EFGKNALAKNAEERYQTGAEMAQALRQCIASLETVDITI"
     gene            complement(1795..2481)
                     /locus_tag="VLL03_02635"
     CDS             complement(1795..2481)
                     /locus_tag="VLL03_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HSS46295.1"
                     /translation="MAEVLTAQFWTGLLTIIWVNIILSGDNAVVIALAARSLRGRQQK
                     QAIIWGGSAAIVIRVILTVFAVKLLTLPSLKIIGGLLLFWIAVKLLLLEEGDGDGIQG
                     SEHLFSAIKTIMIADLVMSLDNVIGVAAAAKGNITLLILGLAISIPLIVFGSTLVLKL
                     MQRFPFIITLGAALLGYIAGELLVTDPLVVEWMELHMPFASIVVSICGALAVVAAGKW
                     LNRSPSAGKA"
     gene            complement(2497..>4039)
                     /locus_tag="VLL03_02640"
     CDS             complement(2497..>4039)
                     /locus_tag="VLL03_02640"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HSS46296.1"
                     /translation="VDSLANKPDGKLILVTAISPTPAGEGKTTTSVGLGDALNRIGKK
                     AVICLREPSLGPVFGMKGGAAGGGYAQVVPMEDINLHFTGDFNAIGLANNLLAAMLDN
                     HIHHGNALGLDVRRIAWRRVVDMNDRALRDIVVSLGGTGNGYPRQDGFDIVVASEVMA
                     IFCLSTSLKDLKNRLGNIVVGYTREQKPVSARDLNAHGAMTVLLKDALNPNLVQTLEN
                     NPAFIHGGPFANIAHGCNSVIATKAALKLADYVVTEAGFGADLGAEKFVDIKCRKAGL
                     RPSAVVLVATLRALKYHGGADPKELNKENLSALEKGIANMERHVNNVRNHYGLPCVVS
                     INHFTFDTDAENALLRKKMAGSQTPVVLARHWAEGSKGAEELAHTVVKMVDSAPNGFK
                     FVYEESATLWDKMKAIATKIYGAADIAADAKVRAQIQKLQDDGYGHYPVCVAKTQYSF
                     STDPSLRGAPGEHVINVREVRLAAGAEFVVMICGDIMTMPGLPKVPSAEKIDLDDDGK
                     VVGLF"
BASE COUNT          871 a         1215 c         1067 g          886 t
ORIGIN      
        1 tctccgcgca ggcggtacag ccgcgaatcg ccgaggtgcg ccacgcttac gcgattatca
       61 taaaacagcc ctgccaccag cgtggtgccc atacccgcat atagcggttg gcttttcgcc
      121 gcatggtaaa tcgaggtgtt ggcgccggct atgtgctctt tcaacatctt caccgccatc
      181 ggctcgccgc tctccggctc gatctgcccc ggatccctcg cctgcagcgc ctgtccaagc
      241 gtggcgccga gcatggcggt ggcaatcccg ctggccactt caccggcatt ataccctccc
      301 atgccgtcgg caagaataac aaatccgcct gcggcatccg ccgcaatact gtcctcgttg
      361 tgcgtgcgca ccatcccggg atcggtggcc atcgcgactt ccagcgcctt gctcaaatcc
      421 atgcgcactc tctcagatcg tgatgtccac ggtttccagg cttgctatgc attgtctgag
      481 cgcttgcgcc atttccgcgc cggtctggta gcgttcctcg gcgtttttcg ccagcgcatt
      541 cttgccgaat tcggcaagtg gaatcagcgc cgcggtcgtg gcgtcaggca gcaagccatc
      601 agacaccgcg gcaattttat cctcgacgtt ggaaagccgg ttcttctcca caaattccgg
      661 cagcggctgg tccggcttgc cgcgcggttc gctgagttgg aacagcaggg cgagcagcac
      721 gttgttcgcc ttcgcaatgc tctcgctaag cttaaggtcg ctgttcaagt tctgctccgc
      781 ttctttgagc agcgtctcga tttgcgacac atcctgttgt accaagggat cggtattgtt
      841 ttttatgccg gaattggcga ggacttggtt gagattgctg atgtagacga ggccaggaac
      901 gacctgcggc tcggaaaaaa ataccgtgtt gccgatcacc ttggctttga cggacgccag
      961 aagatcgatc atcttcgcgt gtacttcgcg cgaccacggc cagcggccga tgttggcgat
     1021 actctggtcg tcaatcgcaa tcaccgccac tttgccgctg gggctgcgcg aggacgcctg
     1081 gacaccgaga tcataagcct tgcgctccag actttggata agatcgctcc cgccggcgaa
     1141 atacagcacg atggcaacaa cgagcccgag gaaccagtca gctttccaga atgcgagctt
     1201 tttcatccct gaactcctta ttatttttat attgtaggtg ggcttttaag ttttagtttg
     1261 ccattttacg atattcattt gtaaagtata cgtttttttg catcgtaaag cgcagtcaca
     1321 tgccggcaaa tcggtttcgg catacagcag ttttactcag ataccccctg ataattctgt
     1381 taaacgataa aatttaaaga aatcaaacat taatccggca ctaaacgata atcaagcgct
     1441 gccttgagct gtgttaaacc acgcaccggt tgcgatccgg tgcaagtgct acatccagtc
     1501 aatcaacaag ggatcgctca cccctatttc tccaacgatg tggccgagcc gcgagcgtaa
     1561 tcatcaccgc attgccgctg gaaaccctga gccggctgaa aaaacacagg aagatctaga
     1621 aacgaaaaag ccggggggct gcacgttcag gaggtgaatc tgcaggggct ccttgtacca
     1681 accttgcttt ttcacaagat aggcggctgc ccggcaggaa ctttgtgatc catcaaccgg
     1741 caaaggcaga ttcagcatag catttccgta acgcgaagat tatcaaaaat tttatcaagc
     1801 tttgccggct gacggactgc ggttgagcca tttgcccgcc gccaccaccg ccaatgcgcc
     1861 gcaaatcgag acgacgatag aggcgaaagg catatgcaac tccatccatt caacgaccag
     1921 cggatcggtg accaacaact cgccggcgat gtagccgagc agcgccgcgc ccaacgtgat
     1981 gatgaacggg aagcgctgca tcagcttgag aaccagcgtg ctgccaaaaa ctatcagcgg
     2041 gatgctgatg gcaagcccga gtatcagcag tgtgatattg cctttggccg ccgccgcgac
     2101 gccgatcacg ttatccaggc tcatcaccaa gtcagcgatc atgatggtct ttatggcgga
     2161 aaacaaatgc tcgctgcctt gaatgccgtc cccatccccc tcttccagca gcaacagctt
     2221 caccgcaatc cagaacagaa gcaggccacc gatgattttg agcgaaggca gtgtgaggag
     2281 cttcaccgcg aacacggtga gaatcacgcg tatcactatg gcggcggaac cgccccagat
     2341 aatcgcctgc ttctgctgcc tgccccgtag cgagcgcgcg gcgagcgcaa ttaccaccgc
     2401 attgtcgccg gacagaatga tgttgaccca gatgatggtc aacaacccgg tccagaactg
     2461 ggccgtcaac acctcagcca tggaactgtt tcctcatcag aacaacccga ccactttgcc
     2521 atcatcatca agatcgattt tctcggctga aggcaccttg ggcaggcccg gcatggtcat
     2581 gatgtcgccg caaatcatca ccacaaattc agcgccagcg gcgagccgca cttcgcgaac
     2641 gttgatcaca tgctcgccgg gcgcaccgcg caaagatgga tcagtggaga aggaatactg
     2701 ggtcttggcg acgcacaccg gatagtggcc gtaaccgtca tcctgcagct tctggatttg
     2761 cgcgcgcacc ttggcgtctg ccgcgatgtc cgccgcgccg taaatcttgg tggcgatcgc
     2821 cttcatcttg tcccacagcg tagccgattc ttcatacaca aatttgaagc cattgggcgc
     2881 gctgtccacc attttcacca cggtgtgggc cagttcctcg gcgcccttgc tgccttccgc
     2941 ccagtgccgg gcgagcacca ccggagtttg gcttcccgcc attttttttc taagaagcgc
     3001 attctccgcg tcggtatcaa aagtgaaatg gttgatggaa accacacacg gcaagccgta
     3061 gtggttgcgc acgttattga cgtggcgctc catgttggcg atgccttttt ccaatgcgga
     3121 aaggttttcc ttgttaagct ctttggggtc cgcgccgccg tgatatttaa gcgcgcgcag
     3181 ggtggcgacc agcaccactg cggaaggacg caagcccgcc ttgcggcact tgatgtcgac
     3241 gaatttctcc gcgcccaggt ccgcgccaaa gccggcttcg gtcaccacgt aatcggcgag
     3301 cttcagcgcc gctttggtgg caatcaccga attgcagcca tgggcgatgt tggcaaacgg
     3361 cccgccgtgt atgaacgccg ggttgttttc cagcgtctgt acgagattgg gattgagcgc
     3421 gtccttcaac agcaccgtca tcgcgccatg cgcgttcaaa tcgcgcgcgc tcaccggctt
     3481 ctgctcacgc gtgtagccga ccacgatgtt gcccaagcgg tttttcaggt ctttaagcga
     3541 agtggaaagg cagaatatcg ccatcacttc agaagccacc acgatgtcaa acccgtcttg
     3601 gcgcgggtaa ccgttgccgg taccgccgag tgacaccacg atgtcacgca aagcacggtc
     3661 gttcatgtcc accactcgcc gccaggcaat gcgccgcacg tccagcccca gcgcatttcc
     3721 gtggtgaatg tgattgtcca gcatcgccgc cagcagatta ttcgcaaggc caatggcgtt
     3781 gaagtcgccg gtgaaatgca gattgatatc ctccatcggc accacttgcg cgtagccgcc
     3841 gcccgcagca ccgcctttca ttccaaacac cgggccgaga gaaggctcac gcaggcaaat
     3901 caccgctttt ttgccgattc gattcaaggc gtcacccagc cccacggaag tggtggtttt
     3961 gccttcgccc gcgggtgtag gactaatagc agtaaccaga atcagcttgc catcaggctt
     4021 attggcgaga ctgtcaaca
//
LOCUS       DATIOW010000084         2195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_950289, whole genome shotgun sequence.
ACCESSION   DATIOW010000084 DATIOW010000000
VERSION     DATIOW010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950289"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /gene="glmM"
                     /locus_tag="VLL03_02645"
     CDS             complement(<1..1181)
                     /gene="glmM"
                     /locus_tag="VLL03_02645"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HSS46297.1"
                     /translation="MARKYFGTDGVRGRVGEPPITPELVMHLGYAAGKVLASADWRLA
                     QGERPSVLIGKDTRISGYMLESALRAGLSAAGVDIYLSGPIPTPAVAYLTRALRLQAG
                     IVISASHNPYEDNGIKFFSASGNKLPDQVERAIEAALDDPIKTMPSAALGRARRINDA
                     AGRYIEFCKSTFPTELDLRGLRIVVDSAQGATYHIAAHVFHELGADVVAIGNQPDGLN
                     INQECGATYPKALQEAVRQQRADLGIALDGDGDRLLMVDGTGKAYDGDQLLYIIAKHR
                     KQQGTLKGGVVGTLMTNLGMQRALEKIKVPFVRAAVGDRYVLEHMQNRGWQLGGENSG
                     HIICLDKHTTGDGIVSALQVLHALRAYRADMAHLTRELALYPQVLVNVKVAKGFDFSN
                     QR"
     gene            complement(1203..2033)
                     /gene="folP"
                     /locus_tag="VLL03_02650"
     CDS             complement(1203..2033)
                     /gene="folP"
                     /locus_tag="VLL03_02650"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HSS46298.1"
                     /translation="MIFLCGRFKLDLSRPLIMGIVNVTPDSFSDGGLHATTEAAVAHG
                     LRLIEEGADMLDIGGESTRPGSLPISLDEELKRVLPVLEALKHVNVPLSADTQKPEVM
                     REALRVGASMINDVNALAVAGALQAVAKSDAAVCLMHKQGEPLTMQVNPQYHDVVAQI
                     KAFLGSRIEAALAAGIARERIVIDPGFGFGKTVGHNLVLLRNLEQFRELGVPLLVGLS
                     RKSVLGKIASREIGERIYSSTAAALIAVMKGAKIVRVHDVGATRDTLAVYNALNAEQY
                     "
BASE COUNT          448 a          684 c          605 g          458 t
ORIGIN      
        1 cgctggttgc tgaaatcaaa tcctttggcg actttgacat tcaccagcac ttgcggatag
       61 agcgcaagct cgcgagtcaa atgcgccatg tccgcccgat aagcgcgcag cgcgtgcaat
      121 acctgcagcg cggagacgat gccatcaccg gtggtatgct tgtcgaggca gatgatgtgc
      181 cctgaatttt ctccgcccag ctgccagccg cggttttgca tgtgctccag cacataacgg
      241 tcgcctactg cggcgcgaac aaacggcacc ttgatttttt ccagggcgcg ctgcatgccg
      301 agattggtca tcagcgttcc caccacgccg cccttgagcg tgccctgctg cttgcggtgc
      361 ttggcgatga tatagagcaa ttgatcgcca tcgtaagctt tccccgtgcc gtcaaccatc
      421 aacagacggt cgccgtcgcc atccagcgcg atccccagat cggcacgctg ctgcctcacc
      481 gcttcctgca gcgccttggg gtaagtagcg ccgcattcct gattgatgtt caaaccatcg
      541 ggctgattgc ctatcgccac cacatccgcg cccagttcgt gaaagacatg cgccgcgatg
      601 tggtatgtcg cgccttgcgc cgagtccacc acgatgcgca ggccgcgcag atccagctcg
      661 gtagggaaag tacttttgca aaattcgata taacgcccgg ccgcatcgtt gattctgcgc
      721 gccctgccca aagcggcgga aggcatggtt ttgatgggat cgtcgagcgc cgcttcaatc
      781 gcgcgctcca cttgatcggg gagcttgttg ccgcttgccg agaaaaactt gatgccgttg
      841 tcttcgtagg gattgtgcga agcggagatc acgatgcccg cctgcaaccg cagtgctcgt
      901 gtcagataag ccactgcggg agtcggtatc ggtccggaaa ggtagatgtc cacacccgcg
      961 gcggacaatc ccgcccgcag cgcggattcc agcatgtagc cggaaatgcg ggtgtctttg
     1021 ccaatcagca cgctgggacg ctcaccttgc gccagacgcc agtctgcgga agccaatacc
     1081 ttgcccgcgg catagccgag atgcataacc agttccggcg tgattggcgg ctcacccacc
     1141 ctgccgcgca cgccgtcggt accaaaatac tttcgagcca taaattctta tgttcggcgt
     1201 ggttaatatt gttcagcgtt caacgcatta taaactgcca gcgtatccct ggttgccccc
     1261 acatcatgca cccgcacgat tttcgctcct ttcatcaccg cgatcagcgc agcagccgtg
     1321 cttgaataaa tccgctcgcc gatttccctg ctagcgattt tacccaaaac tgatttgcgc
     1381 gaaagaccca cgagcagcgg cacgccgagt tccctgaact gctccaggtt gcgcagcaaa
     1441 accagattgt gccccacggt cttgccaaaa ccaaagccgg ggtcaatgac gatgcgttcc
     1501 cgcgctatcc ctgccgcaag cgccgcttcg atgcgggaac ccagaaaagc cttgatctgc
     1561 gcaaccacgt catggtattg cgggtttacc tgcatggtca acggctcgcc ctgcttgtgc
     1621 atcaggcata cagccgcatc gctcttcgcc acggcctgca gcgcgccggc cacggccagg
     1681 gcattgacat cattgatcat gctcgcccct acgcgcaacg cttcgcgcat cacttccggt
     1741 ttctgggtat ctgccgaaag cggcacgttg acatgtttca gtgcttcgag caccggcagc
     1801 acccgcttga gctcctcatc cagcgaaata ggcaatgaac cggggcgggt ggattccccg
     1861 ccgatatcga gcatgtccgc gccctcctca atcaggcgca agccatgcgc gactgcagcc
     1921 tcggttgtgg catgcaagcc gccgtcggaa aaagaatcgg gggtgacatt gacgatcccc
     1981 atgatcaggg gacgggacag gtcaagcttg aaacgcccac acaggaaaat catagtgagg
     2041 agtaaggagc aaggagtgag gggtaaagca tttcacacct taccccttac ctttcactcc
     2101 tcacgaattt cagatttccc gtgatgcagg ctgtggtgtc gccgcggcgg caggaggatt
     2161 gtcctgtgtg gattgaggcg actgcaccgg cttgg
//
LOCUS       DATIOW010000085         2809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_647139, whole genome shotgun sequence.
ACCESSION   DATIOW010000085 DATIOW010000000
VERSION     DATIOW010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647139"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VLL03_02655"
     CDS             complement(<1..620)
                     /locus_tag="VLL03_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HSS46299.1"
                     /translation="MRITHFGKPPKNRNDWNTITTLLPYLWEFRGRVVMALTLLIFAK
                     LANVAVPLALKGIVDSLSLKDALLALPIALLAGYGVLRFSATLFGELRDAVFAKVTQR
                     AVRRVALKVFSHLHSLSLRFHLERQTGGVSRDIERGSRGISFLLQFMLFNILPTFVEI
                     ALVAAILLINYDVWFAVVTFVTLAIYIVFTLVVTEWRMVFRRTMNDS"
     gene            complement(731..1906)
                     /gene="clsB"
                     /locus_tag="VLL03_02660"
     CDS             complement(731..1906)
                     /gene="clsB"
                     /locus_tag="VLL03_02660"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF008427.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HSS46300.1"
                     /translation="MMRLFAGNRVTLLHNGAEYFPELEKAIENSASEIFLEAYIFEND
                     GTGRRIAEALKHAAQRGVTVHLLLDGFGSKSLSHDMLAGMTQAGIQVLLFRPEVLSFK
                     FRRQRLRRMHRKLVEIDGRIAFVGGINIIDDMHTPKQVPPRFDYAVKIEGPLLRVIRA
                     AARDLWARVTWTHLRREWRHTKALPAAVRHCGEQRAALVTRDNLRHRRDIEQAYLNAI
                     AGAKQEIIIANAYFLPGRNFRRALVNAAARGVRVILVLQGRVEYVLLHYASRALYGYF
                     LDAGVEIYEYHKSFMHAKVAVMDGYWATVGSSNIDPFSMLLGREANVVVEDRGFAQQL
                     RQSLMQAIARGARQVVRERWKNQPLAQRILIWISYGVARFLTGMFGYGREQELKLRH"
     gene            complement(1967..2719)
                     /locus_tag="VLL03_02665"
     CDS             complement(1967..2719)
                     /locus_tag="VLL03_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HSS46301.1"
                     /translation="MSKTLHVATYNIHKGFSHFNRRMMVHELRERLHAMHADVVFLQE
                     VQGYHDHHAARYANWPQKPQYEFLADTMWSDFSYGKNSVYTEGHHGNAILSRFPIVHW
                     DNLDVSAHTLENRGMLHCEMKLPGWNEHLHCINVHLGLFSRWREKQLLALRDHIETLV
                     PAQAPLIIAGDFNDWRSQAGRIFADSLNLHEVFELTRGRPARSFPAAMPVFRLDRIYV
                     RGFRVERVHVHRGRPWSKISDHAALSATMMRV"
BASE COUNT          615 a          835 c          794 g          565 t
ORIGIN      
        1 gagtcgttca tggtgcggcg gaacaccatg cgccactccg tcaccaccag ggtaaacacg
       61 atgtaaatgg caagcgtgac gaaagtcacc accgcaaacc acacgtcgta attgatgagc
      121 aggattgccg ccaccagcgc gatttccaca aaggtgggca gaatgttgaa cagcatgaac
      181 tgcagcagga acgaaatgcc gcggctgccg cgctcgatgt cgcgcgacac tcctccggtc
      241 tgccgctcca gatggaagcg cagggaaagc gaatgcaaat ggctgaagac tttgagcgct
      301 acgcggcgga ccgcgcgttg tgtcaccttg gcgaacaccg cgtcacgcag ctcgccaaac
      361 agcgtggcgg aaaaacgcag cacgccataa cctgcgagca gcgcaatcgg caatgccagc
      421 agcgcgtctt tcaagctcaa cgaatcaacg atgcctttaa gcgccaacgg cacagctaca
      481 ttggcgagtt tggcgaaaat cagcagcgta agcgccatca ccactcgccc cctgaactcc
      541 cacagataag gaagcagggt cgtgatggta ttccagtcgt tgcggttctt cggcggcttg
      601 ccgaaatggg tgatgcgcat ggccgttgct tcctaaaatc ttagggtatc accccgtgca
      661 gtgattggcg agccggaaca aacagcgcgg tggcgaagca tgacagcttc ccaatgacgg
      721 tcaaaatcgc ctaatggcgc agtttcaatt cctgctcccg tccataaccg aacatgccgg
      781 tgaggaaacg cgccacgccg tagctgatcc atatcagaat gcgctgcgcg agcggctggt
      841 ttttccagcg ttcgcgcacc acctgccttg cgccgcgcgc aatcgcctgc atcaggcttt
      901 gcctgagctg ctgggcgaag ccgcggtctt ccaccaccac attcgcttcc cgccccagca
      961 acatgctgaa gggatcgatg ttggaggaac cgaccgtcgc ccaatagccg tccatcaccg
     1021 ccaccttggc gtgcatgaag ctcttgtggt actcgtaaat ctccacgccg gcatccaaaa
     1081 aatagccata cagcgcccgc gaagcgtagt gcagcagcac atattccacc cgcccttgca
     1141 ggaccaggat cacgcgcaca cctcgcgccg cagcgttcac cagggcgcgg cggaaattac
     1201 ggccgggaag aaaataagcg ttggcgatga tgatttcctg tttggcgccg gcaatcgcat
     1261 tgagataggc ctgttcgata tcacggcgat ggcgcaagtt gtcgcgggta accagcgccg
     1321 cgcgctgttc tccacagtgc cgaaccgcag ccggcagggc cttggtgtgg cgccattccc
     1381 gccgcaggtg cgtccatgtg actcgcgccc acagatccct cgccgcagca cgaatcaccc
     1441 gcaacagtgg tccttctatt tttaccgcgt aatcgaagcg cggcgggacc tgcttgggcg
     1501 tgtgcatgtc gtcaatgatg ttgatgccgc caacgaacgc aatcctgccg tcgatctcga
     1561 cgagtttgcg gtgcatgcgc cgcagccgct ggcgcctgaa tttgaagctc aagacttccg
     1621 gccggaacaa caggacctgt attcccgcct gggtcattcc agcgagcatg tcatgggaca
     1681 gtgattttga gccgaaacca tccagtagca gatgtaccgt gactccgcgt tgcgctgcgt
     1741 gcttcagagc ttcagcgatg cgcctgccgg tgccatcgtt ttcgaaaata tacgcttcga
     1801 ggaaaatctc agaagccgaa ttctcgattg ccttttccag ctcgggaaaa tattcggctc
     1861 cgttatgcaa cagcgtaacc ctgttccccg caaaaagacg catcattgca ccaaaatatt
     1921 aagctggaag atttgccgca aaatcggtgt gagcgccggc agagctttaa acccgcatca
     1981 tggttgcgga gagggcggcg tggtcggaga ttttcgacca cggtcggccg cgatgcacat
     2041 gcacgcgctc tacgcggaag ccgcgcacgt aaattcgatc cagccggaac accggcatcg
     2101 ccgccgggaa gctgcgtgca ggcctgccgc gggtgagttc gaatacttcg tgcaagttca
     2161 gcgaatcggc gaaaatcctg cccgcctggc tgcgccagtc gttgaaatcg ccggcgatga
     2221 tgagcggcgc ttgcgccggc accagtgttt cgatgtggtc gcgcaatgcc agcaattgct
     2281 tttcgcgcca gcgcgaaaac aggcccagat gcacattgat gcaatgcaga tgctcgttcc
     2341 accccggcag cttcatctca caatgcagca tgccgcgatt ttccagtgta tgcgcggaaa
     2401 catccaggtt gtcccagtgc acgatcggga aacggctcaa aatggcgttg ccgtggtggc
     2461 cttcggtata cactgaattc ttgccgtagc tgaaatcgct ccacatcgta tcggcaagga
     2521 attcgtattg gggtttttgc ggccagttcg catagcgcgc agcatggtgg tcgtgatatc
     2581 cctgtacttc ctgcaggaac actacatctg catgcatcgc gtgcagccgc tcgcgcagtt
     2641 cgtgcaccat catgcgccgg ttgaaatgcg aaaagccctt atggatgttg taggtcgcaa
     2701 catgtaaagt cttgctcatg attgggcgca ttctttagtt agggaacctc tgattaagtc
     2761 ctccgcggag cgcgtttctg gcgcaatgcg cgtgattgcg aggcgcgcg
//
LOCUS       DATIOW010000086         3361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1663682, whole genome shotgun sequence.
ACCESSION   DATIOW010000086 DATIOW010000000
VERSION     DATIOW010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663682"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /gene="hemA"
                     /locus_tag="VLL03_02670"
     CDS             complement(<1..1208)
                     /gene="hemA"
                     /locus_tag="VLL03_02670"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HSS46302.1"
                     /translation="MQLFAVGINHITAPLAMRERVAFHAQTLGEALRDLVNRRPVSEA
                     AIISTCNRTEVYCNTPEPEAAVDWLADYHHLKAQNIQPYVYTLPREKAVKHAFRVASG
                     LDSMVLGEPQVLGQIKQAVRTAEEAGTLGLLLHKLFQRSFSVAKEVRSHTDIGANSVS
                     MAAAAVRLAERIFPTISGQHVLLIGAGEMIELCATHFAARAPRRITVANRTLERAAAL
                     AQRFNGNAITLNELPEQLALHDVVITCTASPLPILGKGMLERAIKMRKHRPMLLIDLA
                     VPRDVEAEAGKLDDVFLYTVDDLAQLVQSGMDARLGAVAQAEAIIESNVISFVHWLET
                     RELVPTIRALRDHAERGRRRELQRALRLLERGADAQLVVESLSRALTNKFLHVPTHVL
                     NHANEDDREQL"
     gene            1367..1687
                     /locus_tag="VLL03_02675"
     CDS             1367..1687
                     /locus_tag="VLL03_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46303.1"
                     /translation="MINYWRLVFTGLGFGGLAVFIAHRRKAFAFASVLAFAGVLGALA
                     VALAFARIHAFTVDLAIFCRKGAADSGCDKHSGRGGGKGNTCELLNCCHGLCLLKIEK
                     CPVL"
     gene            1918..3141
                     /gene="ispG"
                     /locus_tag="VLL03_02680"
                     /gene_synonym="gcpE"
     CDS             1918..3141
                     /gene="ispG"
                     /locus_tag="VLL03_02680"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HSS46304.1"
                     /translation="MQAISRRTSVGVKIGPVTIGGGAPIVVQSMTNTDTADATATATQ
                     VEELATAGSELVRITVNSAEAAQQVARIREQLDQTGCKVPLIGDFHFNGHKLLTEYPD
                     CAAALAKYRINPGNVGRGSKRDEQFATMIEVACKYNKPVRIGVNWGSLDPALLTRMMD
                     ENAELPEPKDANEVTREALIVSALDNAERAEEIGLAHDKIVLSCKVSGVQDLVTVYSE
                     LAERCDYPLHLGLTEAGMGSKGIVASTAALAVLLQRGIGDTIRISLTPEPGGDRTREV
                     IVAQEILQTMGLRSFTPMVVACPGCGRTTSTFFQELADKIQTYLRNQMPVWREQYAGV
                     EKMNVAVMGCVVNGPGESKHANIGISLPGSGETPVAPVFVDGQKTVTLKGANIAEEFQ
                     KIVDDYVRTRYAKLP"
     gene            complement(3144..>3361)
                     /locus_tag="VLL03_02685"
     CDS             complement(3144..>3361)
                     /locus_tag="VLL03_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46305.1"
                     /translation="AVRLSQLMHLVDLIHPEYVYFLPEAASIPFFGKHGESDIRMNTV
                     FLAMPHVTELTAYVVYGAADRLVDFDG"
BASE COUNT          729 a          970 c          996 g          666 t
ORIGIN      
        1 agttgttcgc ggtcgtcctc gtttgcatga ttcagcacat gagtgggaac atgcaggaat
       61 ttgttggtca gcgcgcggct taaggattcc actaccagct gcgcgtccgc gccgcgctcg
      121 aggagccgca gggcgcgctg cagctcgcgg cggcggcctc gttccgcgtg gtcgcgcaag
      181 gcgcgtatgg tgggaacaag ttcgcgcgtt tccagccagt gcacgaaact gatcacgttg
      241 gattcgatga tggcctcggc ctgcgccacc gcgcccaggc gcgcatccat cccgctttgc
      301 accagctgcg caagatcatc cacggtatag agaaacacgt cgtccagttt ccccgcctcg
      361 gcctcgacat cgcgcggcac cgcaaggtcg atcagcagca tggggcggtg cttgcgcatt
      421 tttatcgccc gttccagcat gcctttgccg agtatcggca aggggctcgc ggtacaggtg
      481 atcaccacgt cgtgcaacgc cagctgctcg ggcaattcat taagcgtgat ggcattgccg
      541 ttgaaacgct gcgccagggc ggcggcgcgc tccagcgtgc ggttggcgac ggtaatgcgc
      601 ctgggggcgc gcgccgcgaa atgcgtggcg cacaactcaa tcatttctcc cgcgccgatc
      661 aacagcacat gctgtccgga aatagtgggg aaaatgcgct cggcgagacg cactgccgcc
      721 gccgccatgg aaaccgaatt agcgccgata tcggtgtggc tgcgcacttc cttggccact
      781 gaaaaagagc gctgaaacaa cttatgcagc agcaatccca gggtgccggc ttcttcggca
      841 gtgcgcaccg cctgcttgat ctggccgagg acctgcggct cgcccaatac cattgaatcc
      901 agcccgctcg ccacacggaa agcgtgtttc accgctttct cccgcggcag ggtataaaca
      961 taaggctgga tattctgggc cttgagatgg tggtagtccg ccagccagtc gaccgccgct
     1021 tcgggttcgg gcgtgttgca ataaacctcg gtgcggttac aggtggaaat gatggccgcc
     1081 tcgcttaccg gtctgcggtt caccaggtcg cgcaaggcct cacccaaagt ttgcgcgtga
     1141 aacgccaccc gctcgcgcat cgcaagcggc gcggtaatat ggttgatgcc aacggcaaat
     1201 aactgcatgg ccgtaaccgg gaaagggaat gaaaaacgta gaatattata gcaccggcgc
     1261 gattgggaaa agattacgcc ggtgcccact cgtttcaaaa ggcggcgcat ccaaaagcag
     1321 gaaaagaaaa cggacacccg ccggctaagg cgaaggtgtc cgttaagtga ttaactactg
     1381 gcgcttagtc tttactggcc ttggctttgg cggccttgca gtctttatcg ctcatcgccg
     1441 taaagccttt gcctttgcaa gcgttctggc ctttgcaggc gttcttggcg ctcttgcagt
     1501 cgctcttgcc tttgcacgca ttcacgcctt cacagtggat cttgccatct tctgccgcaa
     1561 aggcgctgcc gattccggct gtgacaaaca ttccggccgc ggcggcggca agggcaatac
     1621 ctgtgagctt ctgaactgct gtcatggttt gtgtctcctg aaaattgaaa aatgcccggt
     1681 tttatagtcc ggcttgcgtc cggactggtt cataatgcga ccgaactgcg cattaatgaa
     1741 ttcgagtgtg tcttggtcgg gaaagttgca attatcttac attaacgcag accggcggtt
     1801 tcagttgacc ggactcgccg ccttggcgcg cagcctgtcc gagtgccggc aacttcaaaa
     1861 tacggtaaaa ttcacggttt ggattcaata aaagttccaa ttatgtcttc accccggatg
     1921 caagcgattt ccagacgcac cagcgtaggc gtgaaaatag gaccagtcac catcggcggc
     1981 ggcgcgccca tcgtggtgca gtccatgacc aataccgata ccgcagacgc aacagcaacc
     2041 gcaacgcagg tggaggagct cgccacagcc ggctcggagc tggtgcgcat caccgtaaac
     2101 agcgccgaag ccgcacagca agtcgcgcgg attcgcgaac agctggacca gacgggctgc
     2161 aaagtgccgc tgatcggcga ttttcatttc aacgggcaca agctgctcac cgaatatccc
     2221 gactgcgccg cagcactggc taaataccgc atcaatcccg gcaacgtcgg cagaggatcg
     2281 aagcgcgatg agcagttcgc caccatgatc gaggtcgcgt gcaaatacaa caagccggtg
     2341 cgcatcgggg tgaactgggg cagcctggat cccgcgctgc tcacgcgcat gatggatgaa
     2401 aacgccgaac ttcccgagcc gaaagatgcc aatgaagtca cccgcgaagc gctgattgtg
     2461 tcggcgcttg ataatgccga gcgtgcggag gaaatcgggc ttgcgcacga taaaatcgtg
     2521 ctctcctgca aagtgagcgg cgtgcaggat ttggtcaccg tatacagcga actcgccgag
     2581 cgctgcgatt atccgctgca tctgggtttg accgaggcgg gaatgggctc caaaggcatt
     2641 gtcgcttcca ctgcggcgct cgccgtgctg ttgcaacgag gtatcggcga caccatacgc
     2701 atctcgctta ctcccgagcc gggcggcgac cgcaccaggg aagtgatcgt ggcgcaggaa
     2761 atcctgcaaa ccatgggact gcgttcattc actccgatgg tcgtggcttg ccccggctgc
     2821 ggacgcacca ccagtacctt cttccaggaa cttgcggata agattcagac ctatttgcgc
     2881 aaccagatgc cggtatggcg cgaacaatat gccggggttg aaaaaatgaa cgtggcggta
     2941 atgggttgcg tggtgaacgg gccgggggag agcaagcacg ccaatatcgg catcagcctt
     3001 cccggctcgg gcgaaacccc cgtggcgccg gtattcgtgg acggccagaa aacggtgacg
     3061 ctcaaaggcg cgaatatcgc cgaggagttt cagaaaatcg ttgatgatta tgtgcggaca
     3121 cgttacgcga agctgccatg atactaaccg tcgaagtcga caagacgatc cgccgcccca
     3181 tagacaacgt acgccgtcaa ttcggtgaca tgcggcatcg cgaggaaaac cgtgttcatc
     3241 cggatgtccg attctccgtg ctttccgaag aagggaatgc ttgccgcttc aggcaggaag
     3301 taaacgtact cgggatgaat caggtcgacg aggtgcatca gctgagacaa cctaacggcg
     3361 a
//
LOCUS       DATIOW010000087         5463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_38383, whole genome shotgun sequence.
ACCESSION   DATIOW010000087 DATIOW010000000
VERSION     DATIOW010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38383"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VLL03_02690"
     CDS             <1..450
                     /locus_tag="VLL03_02690"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013118.4"
                     /note="bifunctional enzyme DHBP synthase/GTP
                     cyclohydrolase II-like protein; functions in riboflavin
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HSS46306.1"
                     /translation="PHGKFRLIAYRDKTANATHLVLLKGEIAPGRETLVRVHEPLSVM
                     DLLDIGAGGHSWSIHDALRKIAGSPCGVMVLLHRPESAEELLMRAKPHAANKKAAKTD
                     LRNYGIGIQILKDLKVTKMRLLAAPRKMPSMAGFGLEVSGYELPDKN"
     gene            451..915
                     /gene="ribH"
                     /locus_tag="VLL03_02695"
     CDS             451..915
                     /gene="ribH"
                     /locus_tag="VLL03_02695"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HSS46307.1"
                     /translation="MAMKKIEKDLNGKNLRIGIIMSRFNAEISDRLLAACTTVLLKHG
                     VSENDITLISVPGALEIPLALQKMANTGKFDALIALGAVIRGETYHFEIVANQSAGGV
                     ASVQLDAGVPVANGILTTDNEKQAQARIQRKGADAAEVAIEMANLLRRLDEK"
     gene            912..1391
                     /gene="nusB"
                     /locus_tag="VLL03_02700"
     CDS             912..1391
                     /gene="nusB"
                     /locus_tag="VLL03_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HSS46308.1"
                     /translation="MKDGEVGAGVSTPRKTATKSNRRRSREFALQALYQWQLGGDSAD
                     VLEQQLREAKGFDKLDSAYFAELLRGVISTARELEKRLKPHLDRPTKELSPIEHGILL
                     LAAYELVHRPEVPYKVVINEAVELAKSFGGTDGHKYVNGVLDKLAAEVRALEVKARA"
     gene            1493..2449
                     /gene="thiL"
                     /locus_tag="VLL03_02705"
     CDS             1493..2449
                     /gene="thiL"
                     /locus_tag="VLL03_02705"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HSS46309.1"
                     /translation="MLSEFELIKRYFTHPARHALLGVGDDAALIKPARGMELAVSTDM
                     LVSGRHFFPDDDACRLGHKALAVNLSDMAAMGAAPRWVTLSLALPKVDEKWLKRFVKG
                     FMDLARKHKVDWIGGDTTRGPLNICVQIMGEVPQGKALRRDAAKPNDDIWVSGFLGDA
                     ALALAHLQKRLRLEPKELQACLPALHTPRPRVNLGKKLRGLAHAAIDISDGLLADLGH
                     ILERSGAGAAVEFNLLPKSGVMEKHAGQKAACQALLAGGDDYELCFTVPARRRGEVQG
                     LSRKLRLRLTRIGKITRSKGLSLLDANGKRMKVDIKGYDHFG"
     gene            2433..2930
                     /locus_tag="VLL03_02710"
     CDS             2433..2930
                     /locus_tag="VLL03_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HSS46310.1"
                     /translation="MTILAEAHPSWRFVLTHPAHFLAFGFGAGLMRAAPGTAGTLVAF
                     PLFWFLNPRLMPLEFLALVAGLFILGWWICGKTGKDLGVHDYGGIVWDEIVAFLLVLY
                     FTPDTLLWQAFAFLLFRIFDVLKPPPIGYFDQTVHGGFGVMLDDLIAASYTLLCLAFW
                     KTLVD"
     gene            2930..3430
                     /locus_tag="VLL03_02715"
     CDS             2930..3430
                     /locus_tag="VLL03_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HSS46311.1"
                     /translation="MANDILYKLAARVGKVLKTRKLMLVTAESCTGGGVGEAVTRVPG
                     SSDWFERGFITYTYISKREMLGVKRRTLGKFGAVSEQIAREMAAGALKMSHAQVAMAV
                     TGIAGPTGGTRERSVGTVCFAWTRKNGGMVSATRHFKGNRQAVRRRSVEHALKGVLAL
                     LARQKK"
     gene            complement(3641..3709)
                     /locus_tag="VLL03_02720"
                     /pseudo
     CDS             complement(3641..3709)
                     /locus_tag="VLL03_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4176..4916
                     /locus_tag="VLL03_02725"
     CDS             4176..4916
                     /locus_tag="VLL03_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46312.1"
                     /translation="MNLPDSVLAAFIGAGATMVAALVQLRMSWRRELRERERGQPITK
                     KTRRGPVLFVFALMIAAAVGGFALSQYFVSLREVDRDALRADLQSKLSEINATAMRLE
                     QARLNERQQIETEAHRADAMQQGAEGAAASVVVGPCKPESAAAGSKPECSEQGAVRVA
                     VCAWVPASATVKEVQVYTRFEDSKQPWADVRVQPGQDAGQARFAEKFSERPDGDAAKQ
                     VCQGFLNWNGEKSRVARILVKYTLQAAN"
BASE COUNT         1122 a         1536 c         1719 g         1086 t
ORIGIN      
        1 ccgcacggca aattcaggct gattgcctat cgcgacaaga cggcgaacgc aacccatctc
       61 gtgctgttaa aaggcgagat cgctcccggt cgggaaacct tggtgcgggt gcacgagccg
      121 ctctcggtca tggatttgct cgatatcggc gcgggtgggc attcatggag cattcatgat
      181 gcgctgagaa aaatcgccgg cagcccatgc ggcgtgatgg tgctgctgca ccggcccgaa
      241 tcagccgaag aacttctgat gcgggcaaag cctcatgccg cgaacaaaaa agcggcgaag
      301 accgatttgc gcaactacgg catcggcatt cagattctca aggatctcaa agtcaccaaa
      361 atgcggctgc tggccgcgcc gcgtaagatg ccgagcatgg cgggcttcgg tctcgaggtc
      421 tccggctacg aattgcctga taaaaactga atggcgatga agaaaataga aaaagactta
      481 aacggcaaaa atctgcgaat cggcattatt atgagccgct tcaacgctga aatcagcgat
      541 agactgctgg cggcctgcac gactgtgttg ctcaaacatg gtgtgagtga aaacgacatc
      601 acgctgatca gtgttcccgg ggcgctggaa attccgctgg cgctgcaaaa aatggcgaat
      661 accggaaaat tcgacgcgct gattgcgctg ggcgcggtga tccgcggtga aacctatcat
      721 ttcgaaatcg tggccaacca gtcggccggt ggtgtggcgt cggtgcagct cgatgccggc
      781 gtcccggtgg cgaacggcat cctcaccacc gataacgaaa aacaggcgca ggcccgaata
      841 cagcggaagg gcgccgacgc tgcggaagtg gcgattgaaa tggccaacct gctgcgccgt
      901 ctggatgaaa aatgaaagac ggcgaagtcg gcgccggcgt aagcacgccg cgcaagacag
      961 caaccaaaag caaccgccgg cgttcgcgcg agtttgcatt gcaggcgctc taccaatggc
     1021 agctgggggg cgatagcgcg gacgttttgg agcagcagct gcgtgaagcg aagggttttg
     1081 acaagctcga ttcggcttac tttgccgaac ttttgcgcgg cgtgatttcc accgcgcgcg
     1141 agctggagaa gaggctcaag cctcatctcg accggccgac caaagaactc agccccatcg
     1201 agcacggcat tttgctattg gccgcttacg aactcgtgca tcggccggaa gtgccctata
     1261 aagtggtcat caacgaagcg gtggaactgg cgaaatcctt cggcggcacc gatgggcaca
     1321 aatatgtgaa tggcgtcctc gacaaactcg cggccgaagt gcgcgcgttg gaagtcaagg
     1381 ccagggcttg aaccacgggg aacacggggt aagcccggtt ttttatgagg gctgttattt
     1441 tgacaaaaca ttatctgtct tccccgtgaa ccccgtggtt gaaatttttt aaatgctttc
     1501 cgaattcgag cttatcaaac gctatttcac ccatccggcg cgccacgcgc tgctgggagt
     1561 gggcgatgat gcggcgctga taaaacccgc caggggcatg gagctggcgg tgtccaccga
     1621 catgctggtt tcaggccggc atttttttcc tgacgacgat gcctgcaggc tcgggcacaa
     1681 ggcgctggcg gtcaacctct ccgacatggc ggcaatgggc gccgccccgc gctgggtgac
     1741 gctgtcgctg gcgctgccca aggtcgacga aaagtggctc aaacgctttg tcaaaggctt
     1801 tatggatttg gccagaaaac ataaggtgga ttggattggc ggggatacca cgcgcggtcc
     1861 gctcaacatt tgcgtgcaaa tcatggggga agtaccgcaa ggcaaggcgc tcaggcgtga
     1921 tgcagcgaaa ccgaacgacg atatctgggt ttccggtttt ttgggtgatg ccgctctggc
     1981 cctggcgcat ttgcaaaagc gtctgcgact tgagccgaag gaattacaag cctgcctgcc
     2041 cgcgctgcat acgcctcgac cgcgtgtgaa cctgggaaaa aaattgcggg gacttgcgca
     2101 tgccgcgatc gatatttccg acggattgct tgccgatttg gggcatatcc tcgagcgttc
     2161 aggcgcgggt gcggcagttg aattcaatct tcttcccaag tccggcgtga tggaaaaaca
     2221 cgcggggcaa aaagcggcct gtcaggcgtt gcttgcaggc ggagacgatt acgagctttg
     2281 ttttaccgtg ccggcgcgcc gccgcgggga agtgcaggga ctttcgcgca aactgcggct
     2341 gcggttaacc cgcatcggca aaatcacacg aagcaagggt ttgtcgttgc tggatgccaa
     2401 cggcaagcgc atgaaagttg acatcaaggg atatgaccat tttggctgaa gcgcatccat
     2461 cctggcgttt tgtgttgacc catccggcgc attttctggc gttcggtttt ggggccgggc
     2521 tgatgcgcgc cgcgccgggc acggcgggga cactggtcgc gtttccattg ttctggtttt
     2581 tgaatccgcg tctgatgccg ctggaattcc tggcgctggt cgccggcctg tttatcctgg
     2641 gctggtggat ttgcggcaag acggggaaag atcttggcgt tcacgactat ggcggcattg
     2701 tctgggacga aatcgtggcg tttctgctgg tgctttattt cacgcccgat acgctgctgt
     2761 ggcaggcttt tgcgtttctc ctgttccgca tttttgacgt actcaagccg ccgccgattg
     2821 gctacttcga ccaaaccgtg cacggcggct tcggtgtgat gctggatgac ctgattgcgg
     2881 cgtcttacac gctgctgtgc ctcgcattct ggaaaaccct ggttgactga tggcaaacga
     2941 tattctctat aaactcgccg cccgcgtagg caaggtgctg aaaacccgta agctcatgct
     3001 ggtgacggcg gaatcctgta ccggcggcgg ggtgggcgag gcggtgacgc gggtgccggg
     3061 aagctccgat tggtttgagc gcggctttat tacttatacc tatatttcca agcgtgaaat
     3121 gctgggcgtg aaaaggcgga cattgggaaa attcggtgcg gtgtcggaac aaatagcgcg
     3181 cgaaatggcg gcaggcgcgc tcaagatgag ccatgcccag gtggcgatgg cggtgaccgg
     3241 catcgcggga cccactggcg gcacgcgcga gagatcggtg ggaacggtgt gttttgcctg
     3301 gacgcgaaag aacggtggaa tggtcagcgc aactcggcat ttcaagggca atcgccaagc
     3361 cgtgcgccgc cggtccgtcg agcatgcgct caaaggcgtg ctcgcgttgc tcgcacgcca
     3421 aaaaaagtaa cgaagtcacg aacgaggtga cccggcacct cgcgccagct acccggtgcg
     3481 gtcgagcggg ctcgactgcg gagggcggag cagtgcctgc aggctcagcc tgtaggtgcg
     3541 accccggagc tggcgcaacg cgtcgggata ttccgcagtc gtaacgcgcg cctccgccgt
     3601 cccatcccca ccaagtgggg cccctgctcc aggctccgac gcgcaccccg cgcccgcctt
     3661 tgttcagcac gtgagtatag atcatcgtgg tggaaacgtc cgagtgccca aagtgcaaag
     3721 ggccaatgcg cgtcattgcg ctaatcgagg atccgggagt catgcagcgc atccttgagc
     3781 acctcggatt gtgggcgccg ctcgccaccg tgcgaagtcc gccgcccgat cccgcgagtt
     3841 ggccccggca tgtcaacctg ctactgacct atcacccggt tccagacatc gcctgacctg
     3901 ctgtacggaa aagaccccct cgaaacagcc ggggtgggga aagcgcttgc cgcgctctcg
     3961 cggttgaagt agattgctcg gtaattgacc gctgatcctg ctcacgaccc gcccggatcg
     4021 cggtcgtctg ccggggaggg aggagccagc gtgaatataa gacccgttat gcgcagtccg
     4081 cgacctcttc cacacccgct tccggcgcgc gggctcggcg aaccgagatc gcggccggat
     4141 tgaatttcct atcgtttcat cgcgtcttcg aaatcatgaa tcttcccgac tcggtactgg
     4201 ccgcgtttat cggcgccggc gccacaatgg tggccgcgct agttcagctt cgtatgtcct
     4261 ggcgcaggga gctgcgagag cgcgagcgcg gacagccgat cacgaagaaa acccggcgcg
     4321 gaccggtgct cttcgtcttt gccctgatga tcgcagcggc ggtgggcggg tttgcgcttt
     4381 cgcaatattt cgtgtcgttg cgcgaagtcg atcgggatgc cctgcgggcc gatctgcaat
     4441 ccaagctctc cgagatcaac gcgaccgcga tgcgactgga acaggcccgc ttgaacgaac
     4501 gccagcagat tgaaaccgaa gctcaccgcg cggatgcgat gcaacaaggc gcagaaggcg
     4561 cggcggcgag cgtcgtggtc ggcccgtgca agccggaaag cgccgccgcc gggtccaagc
     4621 ccgaatgcag cgagcagggc gcagtgcgcg tcgcagtctg cgcatgggtg ccggcctcgg
     4681 cgaccgtgaa ggaagtacag gtttacaccc gcttcgagga ttcgaagcag ccgtgggcgg
     4741 atgtccgcgt acagcccggg caggacgccg gccaggcgcg cttcgcggaa aaattctccg
     4801 agcggcctga cggcgacgca gcgaagcagg tgtgccaggg attcctgaac tggaacggcg
     4861 agaaatcccg cgtcgcgcgg atcctcgtca agtacacgct gcaggccgca aactagcgca
     4921 gcagcacggt gttcgcgtgg tgcgcgtgaa ccggacacca atatgccccc ttgataaagg
     4981 gggcggcgaa cggggtgagc gggggatttt gacgatgcgc aaatgcaaat cccccgccgc
     5041 tgtcgggaaa agcgacaggg tcagccttgg ctaattgcat aaatgaagat gcgtgcgttc
     5101 cgggtggtgg tcgtgacaat atgcaactat gcaagcctgc cccttctctt ctgcatagga
     5161 ctgctcagtg cgcacagcgc cgaacccgga tcgcagtcct gacttgctca agcagctttg
     5221 gcttcgccgc gccagtcgcg ggtgtctcgt ggattgctcg ctccataaac gccatatatc
     5281 tcccaaacgc cgtgtatccg ggtacgctcg aatcggactt ctagataacg ctttgaattt
     5341 gggttacgat gaccaccgat tatggaaatg acgcaattaa ttacgtcttt tcggtcgtct
     5401 actacacgtt agaggtcatg tacaagcgca ccgctgcctt ttttctcgga gccgttgcac
     5461 tcg
//
LOCUS       DATIOW010000088        13551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_141066, whole genome shotgun sequence.
ACCESSION   DATIOW010000088 DATIOW010000000
VERSION     DATIOW010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13551
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141066"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /gene="cydB"
                     /locus_tag="VLL03_02730"
     CDS             complement(<1..654)
                     /gene="cydB"
                     /locus_tag="VLL03_02730"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HSS46313.1"
                     /translation="MIFEYETLKLIWWVFVGLLLIGFAVMGGFDMGVGMLLPFLGKTD
                     EERRAIINSIGATWEGNQTWLITGAGTTFAAWPLVYATAFSGFYIVLMLTLFALFFRP
                     VGFDYRSKLQDRRWRNAWDWGLFAGAAVPTLVFGVAFGNLLLGVPFQLDNDLRITYTG
                     SFWELFNPFGLLAGVVSAAMLIMHGAVYLQNRSEGAIYVRAQKAVKLSAIVLVIAFVL
                     "
     gene            complement(758..2314)
                     /locus_tag="VLL03_02735"
     CDS             complement(758..2314)
                     /locus_tag="VLL03_02735"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012212679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HSS46314.1"
                     /translation="MPSAEVVELSRLQFAVTALYHFLFVPLTLGLSWILVIMESVYVM
                     TGKPIYRDMTQFWGKLFGINFAMGIATGITLEFQFGTNWAYYSHYVGDIFGAPLALEG
                     LMAFFLESTFVGLFFFGWQRLNKVQHLAVTFLVALGSNLSALWILIANAWMQNPVGAE
                     FNYETMRMELTSFPQLFFNPVAGVKFVHTVAAGYVTASLFVLGISSYYLLKNRDTGFA
                     LRSFAVAAGFGLASVLSVIVLGDESGYTTGEVQKVKLAVIEAEWETEEPPAAFTAFGI
                     PNDEKERTDYAVKIPYVLGLIATRSVDEKVLGIKELKAEHLKRIDNGIIAYAALQKLR
                     SGDKSLEALETFEKHKADLGYGLLLKKYTPGVVDATLEQKVKAANDTIPKVAPIFWSF
                     RIMVGLGMLFLFIFSCAFYFCARRSITDQRWLLKLALYSIPLPWVAAELGWIVAEYGR
                     QPWTISDVLPTFLSSSNIAAEKIYFSLTGFVLFYTFLLAVELYLMFKYARLGPSSLHT
                     GRYYSETAHA"
     gene            complement(2301..2501)
                     /locus_tag="VLL03_02740"
     CDS             complement(2301..2501)
                     /locus_tag="VLL03_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46315.1"
                     /translation="MACSTVNKTRSTLARDITLMLIIKAIALYLIWLAWFSSPQDKHL
                     GAVDVGKALISQPAPEPAHAQR"
     gene            2631..5210
                     /gene="clpB"
                     /locus_tag="VLL03_02745"
     CDS             2631..5210
                     /gene="clpB"
                     /locus_tag="VLL03_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HSS46316.1"
                     /translation="MRFDKFTTKFQQALSDAQSNAVGHDNPFIEPQHLLLALLDQEDG
                     GTSSLLGRAGVNVPPLKTALKKSIESLPKVEGNAGEISVSRDLANLLNLTDKEAQKRS
                     DQFIASELFLLALLSDKGETGRLFKQHGADRKHLEQAISAVRGGENVASSEAEGSREA
                     LKKYTLDITERARMGKLDPVIGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPETLKNKRVLSLDMAALLAGAKYRGEFEERLKSVLKELAKDEGQTIVF
                     IDELHTMVGAGKAEGAIDAGNMLKPALARGELHCVGATTLDEYRKHIEKDAALERRFQ
                     KVLVDEPTVEDTIAILRGLQEKYELHHGVEITDPAIVAAAELSHRYITDRFLPDKAID
                     LMDEAAARIKMEIDSKPEEMDKLERRLIQLKIEREAMKKEKDEASRKRLALLESEIKK
                     LEREYADLEDVWKAEKAQVQGAQHIKEELDKLKIEMEAAKRKGDWQKVSELQYGRVPQ
                     LEAQLKQADKVGAQQVKHKLLRTQVGAEEIAEVVSRATGIPVSKMMQGEREKLLHMEQ
                     KLHQRVVGQDEAVRLVSDAIRRSRAGLADPKRPYGSFLFLGPTGVGKTELCKALAEFL
                     FDSEDHLIRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEVE
                     KAHQDVFNVLLQVLDDGRMTDGQGRTVDFKNTVIVMTSNLGSQMIQQMAGDDYQVIKL
                     AVLGEVKAYFRPEFINRIDEVVVFHALDEKNIKAIAKIQLGYLEKRLAQIDIRLQVGD
                     AALDELAKAGFDPVYGARPLKRAIQAQIENPLASALLEGRFAAKDTVKVDCKGGKFIF
                     SKG"
     gene            5269..6306
                     /locus_tag="VLL03_02750"
     CDS             5269..6306
                     /locus_tag="VLL03_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSS46317.1"
                     /translation="MLFRELNQGKCKTYWLACEKTRKALLIDPIREKTDRYLAALAYY
                     GCSLELVIDTHTHADHRTAVFELKELTGARVVMHRNAPAPHIDMHVEDGQAISAGEIR
                     LQVLATPGHTPDSMSLYLDGKVFTGDTLLIRGTGRADFTGGDAGTQYDSITQKLFILP
                     DDTLVFPAHDYRGNTQSSIGEEKRLNPRIANCSRTEYVELMAKLGLPLPTKIQEVLQP
                     NQTALDDDRVAFPSMAELNQVRQLSPLEVQALVNSDSPPLVLDVREPNEYSGELGHIP
                     GSVLIPLKDLANRASELEACKNKHIIAICRAGVRSSTAAAILTSLGFEHVSNLRGGML
                     EWNDKKLPVGR"
     gene            complement(6303..7076)
                     /locus_tag="VLL03_02755"
     CDS             complement(6303..7076)
                     /locus_tag="VLL03_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HSS46318.1"
                     /translation="MLWLHWVLFYNLMMQDTTSPLPGAWVATLTWPEVEKRIQSGAVA
                     VLPVGAAAKEHGRHLLLNTDQLQAEWLAARLIEKANVLVWPTLTYGHYPAFTDYPGSC
                     SLSAATFEKLVEETLQSILGSGIRSALILNTGISTIAPIECVIARMGRQIKLANVYQG
                     RRYLKAEEKIRQQPHGSHADELETSVMLAIAPDQVNMSKAKPWAKREMQPGKLVKNDA
                     SHPNYSPDGVYGDPTLATREKGEKLLQAMLEDVLEMLKG"
     gene            7062..7592
                     /locus_tag="VLL03_02760"
     CDS             7062..7592
                     /locus_tag="VLL03_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSS46319.1"
                     /translation="MQPQHELTAPSEWVSRFASLIPEKGKVLDVACGSGRHARLLAGL
                     GHIVEAVDGDESSLQSLRGVAGVSARLADLEGGPWPYTGRQFAGIIVCNYLHRPLFPR
                     MLEALEQEGVLIYETFAVGNERFGKPSNPNFLLKPGELLEVVRGQLRVIAYEDVYVDH
                     PKPAMVQRICAKRENG"
     gene            7647..8525
                     /gene="dapA"
                     /locus_tag="VLL03_02765"
     CDS             7647..8525
                     /gene="dapA"
                     /locus_tag="VLL03_02765"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HSS46320.1"
                     /translation="MLKGSLVAIVTPMLEDGNLDFPRLRALVDWHIREGTDGIVVVGT
                     TGESPTVDVDEHCELIRVTAEHAARRVPVIAGTGANSTSEAIELTQYAKKVGADYSLS
                     VVPYYNKPTQEGLYRHFKIIAESVNIPQILYNVPGRTSCDLLNPTVLRLAQVPNIVGI
                     KDATGNMERGTDLLRGVPSDFAVYSGDDASALPLMLLGAHGTISVTANVAPKFMHEMC
                     VAAFKGDLATARSINNKLFDLHKNLFVEANPIPVKWAVAQMGLIAPGIRLPLTPLSGA
                     HHETVRNAMRQAGVKV"
     gene            8552..9676
                     /gene="bamC"
                     /locus_tag="VLL03_02770"
     CDS             8552..9676
                     /gene="bamC"
                     /locus_tag="VLL03_02770"
                     /inference="COORDINATES: protein motif:HMM:NF018509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HSS46321.1"
                     /translation="MTRNLRRVAVILVAALALGGCESLPFFGKKDAYKSAQEVRPLEM
                     PPDLTSPGKDERNVETSAQGSATFSDFSKSKTTQVAPGSANVLTTFDTLRIEREGGQR
                     WLVVPAKPETVWPVVRQFWLDNGFVLKTEMPEVGIIETDWAEHRERVPEGGIRGLLNK
                     VINTVRSTSQRDKFRTRLERGVESDTTEIYISHRGMVEVFDNEAKDHTVWQPKDDPEF
                     EAEMLSRLMVRFGSQEAQAKTQIAESAPELVRATFTKRPDGSGALSLNDSFDRAWRRI
                     GLALDRVGFTVEDRDRSQGLYFVRYADPEVTDKQKKGWLDKLVFWKSDEKKIPKNERY
                     RIVVQEAGEQSRVNVLNKDGQPEKSETATRILTLLYEQLR"
     gene            9793..10557
                     /locus_tag="VLL03_02775"
     CDS             9793..10557
                     /locus_tag="VLL03_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSS46322.1"
                     /translation="MRFASLGSGSQGNALVVEVKRTRVMLDCGFGVNETIVRLSRAGL
                     VPEQLDGIVVTHEHADHCGGVAKFARKFNLPVWLTFGTASEFQGEFAALETNYIDSHQ
                     FFSIGDVTLQPYPVPHDAREPAQFVFGDGVRRLGVLTDTGSSTPHIEAVLSGCDALVL
                     ECNHDLEMLMKGSYPPPLKQRIAGRFGHLSNDASARLLASLDGSRLQHLIAAHLSRNN
                     NTPELARAALSSVMNCNPDWIGVADQQEGFAWREIV"
     gene            complement(10624..10821)
                     /locus_tag="VLL03_02780"
     CDS             complement(10624..10821)
                     /locus_tag="VLL03_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46323.1"
                     /translation="MKHSLLVAAILALGLTACGKKEEAAKPAPAPAPAMAPAPAPAPA
                     ADAAKPAEAAKAEEKKGEEKK"
     gene            complement(10951..11445)
                     /locus_tag="VLL03_02785"
     CDS             complement(10951..11445)
                     /locus_tag="VLL03_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46324.1"
                     /translation="MENTAAPQQEKFDSQAGYEAAVNAVIAKAKKRIRIFDKQASPAF
                     NSVKRYELLRNFLLANRDNRLYIVLHDAGFLTANCPRMMLLLRHFSHGIAIHETQQYA
                     KHVYDPFTVIDDSHYAHRFHHDNVRGLMALNDSAGAHEFNDRFDEIWEASFPVVSATT
                     IGLR"
     gene            complement(11464..12591)
                     /locus_tag="VLL03_02790"
     CDS             complement(11464..12591)
                     /locus_tag="VLL03_02790"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSS46325.1"
                     /translation="MKKSFLGGLSPRQFLRRHWQKQPLLIKNAFPGFKELLTREQLFE
                     LACRDDAQARQVMQKRNTWEVFHGPFAAKDFRKLPKRNWTLLVQGVNHFLPQAQRLLL
                     QFNFIPYARLDDLMASYAPPGGGVGPHFDSYDVFLLQGGGRRLWQISAQKDLRLIENA
                     PLKILKKFNAEQEWLAEAGDMLYLPPQYAHNGVALDDCMTYSIGFRAPSAQELAVQFL
                     LYLQDHLHFDGMYRDPGLLPQPRPAQISEAMLRKAARMLRSIKWSGRDVELFFGQYLT
                     EPKPHVIFEPPKRPLAFGDFVRHITSRGAHLDLKSRMLVHRNRIFMNGETCESKGNSF
                     RSLAALADTRELATHAVLDGKMQNLLYQWYAAGYIKIGRTT"
     gene            12674..13144
                     /locus_tag="VLL03_02795"
     CDS             12674..13144
                     /locus_tag="VLL03_02795"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HSS46326.1"
                     /translation="MLQAGQSAPEFDLPDADMQMVSLAGFKNKKYIVLYFYLKDDTPG
                     CTIEAIGFSDLDDEFAQLDTIVLGVSMDDCLSHGSFRDKHGLTVQLLADAEGEVCRKY
                     SVLHDKEAEGRKKSCITRSTFIIDKRGVVRHALYSVNPRGHAHEVLHLIKGMSK"
     gene            13141..>13551
                     /locus_tag="VLL03_02800"
     CDS             13141..>13551
                     /locus_tag="VLL03_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSS46327.1"
                     /translation="MKITKDTVVSLNYELSDASGKLLEKSESPMTYLHGGYYGIFPMV
                     EAALQGKSIGDTCKVTMVPGEAFGDYDVELVCVEPRYRFPENVKVGMQFEGGAESSED
                     VMIYTATGIADGKVVVDANTRLLGKSSIFPVPSPG"
BASE COUNT         3121 a         3717 c         3972 g         2741 t
ORIGIN      
        1 aagcacgaat gcgatgacga gcacaatcgc tgaaagtttt accgctttct gcgcacgcac
       61 gtaaattgcg ccctcgctgc ggttttgcag atacaccgcg ccgtgcatga tgagcatcgc
      121 cgcgctgacc actccggcga gcagtccgaa cggattgaaa agctcccaga acgagccggt
      181 gtaggtgata cgcaaatcgt tatccagctg gaacggcacg cccagcaaga gattgccgaa
      241 agcgacgccg aagaccaaag tgggcaccgc cgctccggca aacaggcccc agtcccaggc
      301 gttgcgccag cggcggtctt gaagtttact acggtaatcg aagcctaccg ggcggaaaaa
      361 cagcgcgaac agcgtgagca tcagcacaat gtaaaaaccg gagaacgcgg tggcgtatac
      421 caacggccag gcggcgaaag tcgtgccggc gccggtaatc agccaggtct ggttgccttc
      481 ccaggttgcg ccgattgaat taatgatggc gcggcgctcc tcgtcggttt tgccgaggaa
      541 gggcaatagc atgcccacgc ccatgtcgaa accgcccatc accgcaaaac caatcagcag
      601 caggccgacg aatacccacc agatgagctt cagcgtttcg tactcaaaga tcatgattag
      661 ctttcgtttt taccgcagag acgccgaggc gcagagaaaa acagacttat catgaattcc
      721 ttcgcgtctc tgcacctccg cggttcaaaa ttctgcatca tgcgtgtgcc gtctcggaat
      781 aataacggcc ggtgtgcaga ctgctcggcc ccagccgcgc gtacttgaac atcaaatata
      841 attccaccgc cagcaagaaa gtgtaaaaga ggacgaaccc ggtaaggctg aaataaattt
      901 tttccgccgc aatgttcgag gagctgagaa acgtcggcag cacgtctgaa atggtccacg
      961 gctggcggcc gtattcggcg acgatccagc cgagttccgc cgctacccag ggtagcggaa
     1021 tggagtaaag cgcgagcttc aacagccagc gctgatcagt tatcgaacgc cgggcgcaga
     1081 aataaaacgc gcagctgaaa atgaacagaa acagcattcc cagcccgacc atgatgcgga
     1141 acgaccagaa gatgggcgcc actttcggaa tcgtgtcgtt cgctgctttc actttctgtt
     1201 ccagcgtggc gtccaccacg ccgggcgtgt atttcttgag cagcaaacca tagcccaggt
     1261 cggccttgtg tttttcaaat gtctccagcg cctccaggct tttatcgcca ctgcgcagtt
     1321 tctgcagcgc tgcgtaggcg atgatgccgt tgtcaatgcg cttgaggtgt tccgccttga
     1381 gctctttgat gccgagcact ttttcatcca ccgagcgagt cgcgatcaat cccagcacgt
     1441 aggggatctt gacggcatag tcggtgcgct ccttttcgtc attgggaatg ccgaaagcgg
     1501 tgaaagcggc gggcggctcc tcggtttccc attccgcttc aatcaccgcc agcttcactt
     1561 tttgcacttc gccggtggta tagccgcttt cgtcgccgag cacgatgacc gaaagcacgg
     1621 aagcgagccc gaatccggca gccaccgcaa acgaacgcag cgcaaatccg gtatcgcggt
     1681 ttttaagcag gtagtagctc gaaatgccca aaacaaacag cgaggcggtg acataacctg
     1741 ccgccaccgt gtgcacgaat ttcacccccg ccaccggatt gaagaaaagc tgagggaaac
     1801 tggtcaattc catgcgcatg gtttcgtaat tgaattccgc gccgaccgga ttctgcatcc
     1861 aggcgttggc aatcaatatc cacagcgccg agaggttgga gcctagcgcc accaggaaag
     1921 tcacagcgag gtgctgcact ttattcagcc gctgccaacc gaagaaaaac aggccgacga
     1981 aagtggattc gaggaagaac gccatcagcc cctcgagggc caggggcgcg ccgaaaatat
     2041 cgcccacgta atgcgagtaa tacgcccagt tggtgccgaa ctggaattcc agcgtgatgc
     2101 cggtcgcgat gcccatcgcg aaattgatgc cgaagagctt gccccagaac tgcgtcatgt
     2161 cgcggtaaat cggttttccg gtcatcacgt agacggactc catgatgacc agaatccacg
     2221 acaagcccag cgtcaagggc acgaacagga agtggtaaag cgcggtgacc gcgaactgca
     2281 gccgcgacag ctcgacgact tcagcgctgg gcatgggcgg gttccggcgc gggctggctg
     2341 atcagggctt tgccaacgtc aacagcgcca agatgcttgt cttgcgggct ggaaaaccag
     2401 gcgagccaga tgagataaag tgcgatcgcc ttgataatca acatcagcgt gatgtcgcgc
     2461 gcaagggtgg aacgggtctt gttcacggta gaacatgcca tgaagggttt tttcaagtat
     2521 aaccgaagcg actgtgctca agaacggtgt taatctaatg cgcggcgagt tgaaatcatg
     2581 aatctgcccc ccatttagtc tgcgaatcct taaatattaa taggctagac atgcgttttg
     2641 acaaatttac caccaagttt cagcaggcgc tgtccgacgc gcagagcaat gccgtcgggc
     2701 atgacaaccc gtttatcgag ccgcagcatc tgttgctggc gctgctcgac caggaggacg
     2761 gcgggaccag ttcgctcctc gggcgggccg gagtcaatgt gccgccgctc aaaaccgcgc
     2821 tcaagaaaag catcgagagc ctgcccaagg tcgaaggcaa tgccggcgaa atcagcgtct
     2881 cgcgtgatct ggcgaacctc cttaatctta ccgacaagga agcgcagaag cgtagcgacc
     2941 agtttatcgc ttccgaactt ttcctgctgg cgctgctctc ggacaagggc gaaaccggcc
     3001 ggctgttcaa gcagcacggc gcggaccgca agcatctgga acaggcgatt agcgcagtgc
     3061 gcggcgggga gaacgtggcc agttccgaag cggaagggag ccgtgaagcg ctcaaaaaat
     3121 acacgctaga tatcaccgag cgcgcgcgca tgggtaagct cgatcccgtc atcgggcgtg
     3181 atgatgaaat ccgccgcacc atccaggttt tgcagcggcg cactaagaac aatccagtct
     3241 tgatcggcga gccgggagtg ggcaagaccg cgattgtcga gggcctggcg cagcgcatcg
     3301 tcaacgggga agtgcctgag acgctcaaga acaagcgcgt actctcgctc gacatggcgg
     3361 cgctgcttgc cggcgcgaaa taccgcggcg aattcgaaga acggctgaaa tcggtgctga
     3421 aggaactcgc caaagacgag ggccagacga tcgtatttat cgacgagctg cacaccatgg
     3481 tcggcgccgg caaggcggaa ggcgcgatag acgccggcaa catgctgaaa ccggcgctgg
     3541 cgcgcggcga gctgcattgc gtgggcgcga ccacgctcga cgaataccgc aaacacatag
     3601 aaaaggacgc ggcgctggag cggcgcttcc agaaagtgct ggtggatgag ccgacggtgg
     3661 aagacaccat cgccatcctg cgcggcctgc aggaaaaata cgaattgcat cacggcgtag
     3721 aaatcaccga cccggccatc gtcgccgcgg cagagctttc gcaccgctac atcaccgacc
     3781 gctttcttcc agacaaggcg attgatctga tggacgaagc ggcggcgcgc atcaagatgg
     3841 agattgactc caagccggaa gaaatggaca agctggagcg gcgcttgatc caactgaaaa
     3901 tcgagcgcga ggcgatgaag aaagaaaaag acgaagcctc gcggaagcgc ttggcgttgt
     3961 tggaaagcga aatcaagaag ctcgaacgcg aatacgccga ccttgaagat gtctggaaag
     4021 cggaaaaagc gcaggtacag ggtgcacagc acatcaagga agaactcgac aagctcaaaa
     4081 tcgaaatgga agccgccaag cgcaagggcg actggcagaa agtttccgaa ttgcaatatg
     4141 ggcgcgttcc tcagctcgaa gcgcagttga agcaggccga caaggtgggc gcacagcaag
     4201 tgaagcacaa actgctacgc acccaggtcg gcgcggagga aatcgccgag gtagtctcgc
     4261 gcgcaaccgg aattcctgtc tcgaaaatga tgcagggcga gcgcgaaaaa ctgctgcaca
     4321 tggagcaaaa actgcaccag cgcgtggtgg ggcaggatga agcggtgcgc ttggtgtcgg
     4381 acgcaattcg ccgctcgcgc gccgggctgg ccgatccgaa gcggccttac ggttcattcc
     4441 tgttcctggg cccgaccggc gtgggcaaga ccgagctttg caaggcgctc gccgaattcc
     4501 tgtttgattc ggaagatcat ctcattcgca tcgatatgtc ggaattcatg gagaagcatt
     4561 cagtcgcgcg gctgatcggc gcgccgcccg gctatgtcgg ctatgaagag ggcggctatc
     4621 tcaccgaggc ggtgcggcgt aagccttatt cggtgattct gctcgacgaa gtggaaaaag
     4681 cgcatcagga cgtgttcaac gtgctgctgc aggtgctcga cgacgggcgc atgactgacg
     4741 gccagggacg taccgtggat ttcaagaata cggtgattgt gatgacctcc aacctcggct
     4801 cgcagatgat ccagcagatg gcgggcgacg actaccaagt catcaagctc gcggtgctgg
     4861 gcgaggtgaa ggcttatttc cgtcccgaat tcatcaaccg cattgatgaa gtggtggtgt
     4921 tccacgcgct ggatgaaaag aacatcaagg cgattgccaa aatccagctt ggctatctgg
     4981 agaagcggct ggcgcaaata gatatccggc tccaggtcgg cgatgcggcg ctggacgagc
     5041 tcgccaaagc cggattcgat ccggtctatg gcgcgcgccc cttgaaacgc gcgatccagg
     5101 cgcaaatcga aaacccgctg gcatcggcgc tcctcgaagg ccggtttgcc gccaaggaca
     5161 ccgtcaaagt ggattgcaag ggcggcaagt tcatcttcag caagggttga acggcaaccc
     5221 gcaccaagcc ctataatgga gtagtcactc atctccatag agggttgcat gctttttcgc
     5281 gaacttaatc aaggcaaatg caaaacctat tggctggcct gtgaaaagac ccgcaaagcg
     5341 ctgttgattg accccatccg ggaaaagacc gatcgctacc tggctgcgct agcgtattac
     5401 ggttgttctc tcgagctggt gatagacacg catacgcatg ccgatcatcg cactgcggtg
     5461 tttgagctta aagaactcac cggcgcgcga gtcgtcatgc accgcaacgc acctgcgccg
     5521 catatcgata tgcatgtcga agatggccag gccatctccg cgggagaaat caggctgcaa
     5581 gtattggcaa cgccgggcca tactcccgat agcatgagtc tttacctgga cggcaaggtt
     5641 tttaccggcg atacgctgct gattcgcggt accggacgtg ccgatttcac cggcggcgat
     5701 gccgggacgc agtacgacag cattactcaa aagctgttta ttttgcctga cgacaccctg
     5761 gtgtttcccg cgcatgacta ccgcggcaac actcaatcca gcatcggcga agagaagcgc
     5821 ctgaatcccc gcattgccaa ctgctccaga accgagtatg tggaattgat ggcaaaactc
     5881 ggtttgcctt tgccaaccaa aatccaggaa gtgctgcagc ctaaccaaac cgcgctcgat
     5941 gacgatcgcg ttgcctttcc cagcatggcc gagctcaatc aggtgcggca gttgagccct
     6001 ctcgaggtgc aggcgctggt taacagcgat tcaccgccgc tggttctcga tgtgcgcgag
     6061 ccgaatgaat actccggcga gttggggcat attccgggaa gcgtgctgat tccgttaaaa
     6121 gacctcgcca accgcgcaag cgaattggag gcatgtaaga acaagcacat catcgccatt
     6181 tgccgcgccg gcgtgcgcag cagcaccgca gcggcaatac tcaccagcct gggtttcgag
     6241 cacgtgtcaa atcttcgcgg cggaatgctg gagtggaacg acaaaaaatt gcctgtgggg
     6301 cgttaaccct tgagcatttc aagtacatct tcaagcatgg cttgcaacag cttttcgcct
     6361 ttttccctcg ttgccagcgt cggatcgccg taaactccgt ccggcgaata attggggtgg
     6421 gatgcatcgt ttttaaccag tttgccgggc tgcatctccc gtttggccca aggcttagct
     6481 ttgctcatgt ttacttgatc cggcgcgatg gcgagcatta cggaggtttc caactcgtcg
     6541 gcatggctgc catgaggctg ttgcctgatt ttttcttccg ctttcaggta gcgcctgcct
     6601 tgatacacat tcgcaagttt gatttggcgg cccatccgtg caatcacgca ctcgatagga
     6661 gcgatggtac tgatgccggt attcaggatt aacgccgacc tgattccgct gccgagaatg
     6721 ctctgtaaag tttcttccac cagtttttca aaggttgcgg cagacaggct acagctaccg
     6781 ggataatcgg tgaacgcggg ataatggccg taagtcagtg tcggccacac cagcacgttg
     6841 gccttttcga taaggcgcgc agccagccat tccgcttgca gttgatcggt gttgagaagc
     6901 aaatgcctgc catgctcttt cgccgccgcg cccaccggca ataccgcaac tgcgccggac
     6961 tgaatgcgct tttcgacctc cggccaggta agcgtcgcca cccatgcgcc gggaagaggg
     7021 cttgtggtat cttgcatcat caaattgtag aacagaaccc aatgcagcca cagcatgaat
     7081 taaccgcgcc ttcggaatgg gtgagccgct ttgcgagctt gattccagaa aaaggaaaag
     7141 tgctggatgt ggcctgcggc tcgggccgtc acgcgcggct gcttgccgga ctggggcata
     7201 ttgtcgaggc ggtggacggg gatgaatctt ccctgcaaag cctgcgcggt gtcgcgggag
     7261 tgtcggctag actcgccgac ctggaaggcg ggccgtggcc ctataccggc cggcaattcg
     7321 ccggaattat cgtatgtaat tatctgcacc gcccgctttt cccccgtatg ctggaagccc
     7381 tggagcagga gggagttctg atttacgaaa cctttgcggt gggcaatgag cgcttcggca
     7441 agccgagcaa cccgaatttt ttgttaaaac ctggggaatt actggaagta gtgcgcggtc
     7501 aattgcgcgt gattgcctat gaagatgttt atgttgatca tcccaaaccg gcaatggtgc
     7561 agcgtatctg cgccaagcgt gaaaacggtt aagaaccagc gggaatttaa tgtaaaatat
     7621 cggcttttat tatcaggtaa agagtaatgc tcaaaggcag tctggtggcg attgtcaccc
     7681 cgatgctgga agacggcaat ctcgattttc cacggctgcg cgcgctggtg gactggcata
     7741 tacgcgaagg caccgacggc atcgtggtgg tgggcaccac cggcgaatcg ccgacggtgg
     7801 atgtggatga gcattgcgag ctgattcgtg tgaccgccga acatgctgcg aggcgtgttc
     7861 cggtgattgc cggcaccggc gccaattcca cttccgaagc gattgaactc actcaatatg
     7921 cgaaaaaagt cggcgcggat tacagcctgt cggtggtgcc gtattacaac aagccgaccc
     7981 aggaaggtct gtaccggcat ttcaagatca tcgcggaaag tgtgaacatt ccgcaaatcc
     8041 tctacaatgt tcccggcaga acatcttgcg atttgctcaa tccaaccgta ttgaggctgg
     8101 cgcaggtgcc gaatatcgtc ggtatcaagg acgcgacggg caacatggag cggggcacgg
     8161 acttgctgcg cggcgtgcct tcggatttcg cggtttacag cggcgatgac gcgagtgcct
     8221 tgccgctcat gctgctgggc gcgcacggca cgatttcggt caccgccaac gttgcgccga
     8281 aattcatgca tgagatgtgc gtggcggcgt tcaagggcga tctggcgacg gcgcgcagca
     8341 tcaacaacaa gctattcgat ttgcacaaga atttatttgt cgaggcgaac cccattccgg
     8401 tgaaatgggc ggtggcacaa atgggcctca ttgcgccggg catccggctg ccgcttaccc
     8461 ccctgtccgg cgcgcatcat gaaacagtgc gcaacgcgat gcgacaggcc ggggtcaagg
     8521 tttaagtggg atgatgacaa aagctcaagg tatgacgcga aatttgcgac gggtagcggt
     8581 gatactggtt gctgcgcttg cgttaggcgg ttgcgaatca cttccctttt tcggcaagaa
     8641 ggatgcttac aagtcggcgc aggaagtgcg gccgctggaa atgccgccgg atctcacctc
     8701 gcccggcaaa gacgagcgca acgttgaaac cagtgcgcag ggcagcgcta ctttttccga
     8761 tttcagcaag agcaagacta cgcaggttgc tccaggttcg gcgaacgtcc tgaccacgtt
     8821 tgacacgttg cgcattgagc gcgagggcgg gcagcgctgg ctggtggtgc ccgccaagcc
     8881 ggaaacggtt tggcccgtgg tcaggcagtt ctggctggac aacggctttg tgctcaaaac
     8941 ggaaatgcct gaagtcggca taatagaaac cgattgggcg gagcaccgcg agagagtgcc
     9001 tgaaggggga atacgcggtt tgctcaataa ggtaatcaat accgtgagat caacgtcgca
     9061 gcgcgacaaa ttccgcaccc gcctggagcg cggcgtcgag tccgacacca ccgaaattta
     9121 tatcagccat cgcggcatgg ttgaagtatt cgacaacgaa gctaaagacc acacggtatg
     9181 gcagcccaag gatgatcccg aattcgaagc ggaaatgctc tccaggctca tggtgcgctt
     9241 cggcagccag gaagcccagg ccaaaactca aatagcggaa tcggcgcccg agcttgttcg
     9301 cgcgacgttc accaaaagac ctgacggcag cggcgcgctt tcactcaatg acagttttga
     9361 tcgtgcctgg cggcgcatcg gcctggcgct ggatcgtgtc ggcttcaccg tggaagaccg
     9421 cgaccgctcg caagggcttt atttcgtgcg ctacgccgac ccggaggtca ccgacaagca
     9481 gaagaagggg tggcttgata aactggtgtt ctggaagtcg gatgagaaaa aaattcccaa
     9541 gaacgagcgc taccgcatcg tcgtgcagga agctggtgag caaagccggg tcaatgtgct
     9601 gaacaaggac ggtcagccgg aaaagtccga aaccgccacg cggattttga ccttgcttta
     9661 cgagcaacta aggtaattca caaggagtaa ggggaaagga gtgaggagta aacaacgaaa
     9721 gccaaggcca ctcatgaaaa ttttctcctc gctttacgct cctcacccct tacgcctcac
     9781 ccctcacgca tgatccgttt tgcctccctc ggcagcggca gccaggggaa cgccctggtg
     9841 gtcgaagtca agcgcaccag ggtgatgctg gattgcggtt tcggcgttaa cgaaaccatt
     9901 gttcggcttt cccgggcggg gctcgtcccc gagcagcttg acggcatcgt ggtcacccat
     9961 gagcacgcgg atcattgcgg cggcgtggcc aaattcgcgc gcaagttcaa tttgccggtg
    10021 tggctgactt tcggcaccgc aagcgagttt cagggcgaat ttgccgccct ggagaccaat
    10081 tacatcgaca gccaccagtt cttcagcatc ggtgacgtta cgcttcagcc ctatccggtg
    10141 ccgcatgacg cgcgcgagcc agcgcagttt gtgttcggcg acggcgtcag gcggctcggc
    10201 gtactcaccg acaccggctc ttccacgccc catatcgaag cggtattaag cggctgtgac
    10261 gctctggtgc tggaatgcaa tcacgacctt gagatgctca tgaagggaag ctacccgccg
    10321 ccgcttaagc agaggattgc cgggcgcttt ggccatttat ccaacgacgc ttcggccaga
    10381 ctcctggcga gccttgatgg cagtcgcctg cagcatctca ttgccgcgca tttgagccgg
    10441 aacaacaaca cgcccgagct ggcgcgagcc gcgctttcga gcgtgatgaa ctgcaacccg
    10501 gactggattg gcgtggcgga ccagcaggag ggattcgctt ggcgcgagat tgtgtaagga
    10561 acctctgatt tcaggctaaa aaaagccggc gcgaacgccg gctttttcat tgccgtgaga
    10621 gaactacttc ttttcttcgc ctttcttctc ttcggctttg gctgcttcgg cgggtttggc
    10681 cgcatcagca gcgggggcgg gggcgggagc cggagccata gccggggcgg gagccggagc
    10741 aggtttggcg gcttcttctt tcttgccgca agcggtcagg cccagtgcca gaatggcagc
    10801 aacaaggagc gaatgtttca tttataactt tccttaagta aatgtttagg atctaaaagc
    10861 aattcagcga tcggccgcct aacagacgtt gctaacggcc gagtcgcaat tttaacaaat
    10921 tttcaacgtg catgcgacag cttaggggga tcagcgcagg cctatggtgg tggcggaaac
    10981 gaccgggaaa gacgcctccc agatttcgtc gaaacggtca ttgaactcat gcgccccagc
    11041 ggaatcgttg agcgccatca agccacgcac attgtcgtga tgaaagcggt gcgcgtaatg
    11101 gctgtcgtca atgactgtga acgggtcgta aacatgcttg gcgtactgct gggtttcatg
    11161 gatagcgatg ccgtggctga aatgcctgag cagcagcatc attcgcgggc agttcgcggt
    11221 cagaaatccc gcgtcgtgca gcacgatgta caggcgattg tcccggtttg ccagcaggaa
    11281 atttcgcagc aactcatagc gtttgaccga attgaaagca ggactcgcct gcttgtcgaa
    11341 aatgcggatg cgcttcttcg ctttggcgat gaccgcgttt accgcggctt cgtagcccgc
    11401 ttggctgtcg aatttttcct gttgcggcgc agcggtgttt tccatggctc aagtccagca
    11461 aattcacgta gtacggccaa ttttaatata gcctgcggca taccattggt aaagcaggtt
    11521 ctgcatttta ccgtcgagaa cggcgtgcgt tgcaagctca cgcgtgtcgg caagcgcggc
    11581 aaggctgcgg aagctgttgc ctttagattc acaagtttca ccgttcataa aaatcctatt
    11641 gcggtgaacc agcatccggc ttttcagatc gagatgcgcc cccctggatg tgatatgacg
    11701 cacgaaatcc ccgaaagcca gcggccgttt gggaggttcg aaaataacgt ggggcttggg
    11761 ctcggtgagg tattgcccga aaaaaagctc gacgtcgcgc ccgctccatt ttatagacct
    11821 taacatgcgc gcggccttgc gcagcatcgc ttcgctgatt tgcgccggtc gaggctgtgg
    11881 cagcaggccg ggatcgcggt acatgccgtc gaaatgcaga tgatcctgca ggtaaagcag
    11941 aaactggacg gcaagctcct gtgcggaagg cgcgcgaaag ccgatggaat aagtcatgca
    12001 atcatccagc gccacgccgt tgtgcgcgta ttgcggcggc agatacaaca tgtcgcccgc
    12061 ttccgccagc cattcctgtt cagcgttgaa ttttttcagg attttcagcg gggcgttttc
    12121 tatcagccgt agatcttttt gcgcgctgat ttgccaaagc ctgcggccgc cgccttgcag
    12181 cagaaacaca tcgtaggaat cgaaatgcgg gcccacgccg ccgccaggcg gcgcatagct
    12241 cgccatcaag tcatccagcc gcgcgtaggg aataaaattg aattgcagca agaggcgctg
    12301 cgcttgcggc agaaaatgat tgactccctg caccaacaag gtccagttgc gtttgggcag
    12361 cttgcggaaa tcctttgcgg caaacgggcc gtgaaatact tcccaggtgt tgcgtttttg
    12421 catcacctgc cgcgcttgcg cgtcatcgcg gcaagccaat tcaaacagct gttcccgcgt
    12481 gagcagctcc ttgaaacccg ggaacgcgtt tttgatcagc agcggttgct tttgccaatg
    12541 ccggcgcagg aattgtcgcg gcgacaggcc gccgagaaaa gattttttca tggttgcaca
    12601 ttaccgttct gtcaaaacca tttcaagttt cgtgaaatgc gctaaaatca aattacatgt
    12661 tttaggagca gttatgttgc aggcaggaca atccgcaccc gaatttgatc tgcccgacgc
    12721 tgacatgcag atggtcagtc ttgccgggtt taaaaacaaa aaatatattg tgctttattt
    12781 ttatctgaaa gacgatacgc cgggttgcac catcgaggct attggtttca gcgacctcga
    12841 cgacgaattt gcccagcttg acaccatcgt gctcggcgtg agcatggatg actgcttaag
    12901 tcacggctcc ttccgcgaca aacacggtct caccgtgcag ctgctcgccg atgccgaagg
    12961 cgaggtgtgc cgcaagtaca gcgtattgca tgacaaggaa gccgaaggca ggaagaaaag
    13021 ctgcatcacc cgttccacct tcattattga taagcgcggc gtcgtgcggc acgccctcta
    13081 cagcgtcaac cctcgcggcc atgctcacga ggtgctgcat cttataaagg ggatgtcaaa
    13141 atgaaaatca ccaaagatac ggtggtctcc ctcaactatg aacttagtga cgccagcggc
    13201 aagcttctgg agaaaagcga atcgcctatg acctacctgc atggcggcta ctacggcatt
    13261 ttcccgatgg tggaagcggc gctgcaaggc aagagcatag gcgatacctg caaggtcacc
    13321 atggtaccgg gagaagcgtt cggcgattac gacgtcgagc tcgtgtgcgt cgagccgcgc
    13381 tataggtttc cggagaacgt caaggttggg atgcagttcg aaggcggcgc cgaaagctcg
    13441 gaggatgtga tgatttatac cgcgaccggc atcgccgacg gcaaggtggt ggtggatgcc
    13501 aacacccgct tgctgggcaa atcctcaatt tttcctgtac cgtcaccggg g
//
LOCUS       DATIOW010000089         4393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1461943, whole genome shotgun sequence.
ACCESSION   DATIOW010000089 DATIOW010000000
VERSION     DATIOW010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461943"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..112
                     /locus_tag="VLL03_02805"
     CDS             <1..112
                     /locus_tag="VLL03_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HSS46328.1"
                     /translation="DKIDALRGLNITITTTAKSDDEARALLAAFKFPFKN"
     gene            120..425
                     /gene="rpsN"
                     /locus_tag="VLL03_02810"
     CDS             120..425
                     /gene="rpsN"
                     /locus_tag="VLL03_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HSS46329.1"
                     /translation="MAKTSVVNRELKRRAVVKKFTAKRAGLLALISNMRLSEEERHQA
                     RLKLQQLPRNSSPVRLRNRCALTGRPRGYYRKFGLGRNKLREIAMRGEIPGIIKASW"
     gene            439..834
                     /gene="rpsH"
                     /locus_tag="VLL03_02815"
     CDS             439..834
                     /gene="rpsH"
                     /locus_tag="VLL03_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HSS46330.1"
                     /translation="MSMTDPISDMLTRIRNAQLSEKTTVILPSSRLKAAIAQVLKDEG
                     YIEGFAVREVEGKTTLEIALKYYAGRPVIEKIERVSRPGLRIYRGSRDIPNVMNGLGI
                     AIISTPKGVMTDRKARANGVGGEVLCIVA"
     gene            848..1381
                     /gene="rplF"
                     /locus_tag="VLL03_02820"
     CDS             848..1381
                     /gene="rplF"
                     /locus_tag="VLL03_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HSS46331.1"
                     /translation="MSRIAKNPVVVPAGVEVTLSPSEVAVKGPLGQLKQHLKGDVLIE
                     REGDRLKFKVGNQSKHANVMSGTLRAHLANMIHGVTKGFEKKLSLVGVGYRAQAQGDK
                     LNLSLGFSHPVVHRMPKGIKVETPSLTDIVIKGIDRQLVGQVTAEVRGYRKPEPYKGK
                     GVRYVDEVVLLKETKKK"
     gene            1394..1744
                     /gene="rplR"
                     /locus_tag="VLL03_02825"
     CDS             1394..1744
                     /gene="rplR"
                     /locus_tag="VLL03_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009277655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HSS46332.1"
                     /translation="MDDKKQSRLRRSRKTRAKIHELRMMRLTVHRTSNHIYAQVVNEG
                     KVVATASTLEPEVRKKVKYGGNVKAASVIGKRIAEKARKQGIESVAFDRSGFKYHGRV
                     KALADAARENGLKF"
     gene            1756..2268
                     /gene="rpsE"
                     /locus_tag="VLL03_02830"
     CDS             1756..2268
                     /gene="rpsE"
                     /locus_tag="VLL03_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HSS46333.1"
                     /translation="MAKMQHRSEQRSDGLREKMVAVNRVTKVVKGGRILGFAALTVVG
                     DGDGGVGMGKGKAREVPVAVQKAMDEARRKMIKITLKNGTLQHAVIGKHGSAKVYMQP
                     AIEGTGIIAGGPMRAIFEVMGVTNVLAKCLGSTNPYNVVRATLQGLQAMSTPSEIAAK
                     RGKTVEQILE"
     gene            2272..2484
                     /gene="rpmD"
                     /locus_tag="VLL03_02835"
     CDS             2272..2484
                     /gene="rpmD"
                     /locus_tag="VLL03_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006411099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HSS46334.1"
                     /translation="MVRKKAAGKIKAAGKIKVTLVKSPQSAKESHQATVRGLGLRRLR
                     HTVELEDTPAVRGMINKVGYLLKCEA"
     gene            2485..2931
                     /gene="rplO"
                     /locus_tag="VLL03_02840"
     CDS             2485..2931
                     /gene="rplO"
                     /locus_tag="VLL03_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HSS46335.1"
                     /translation="MELNGIKPAAGSKRERRRVGRGPGSGMGKTAGRGHKGQRARSGG
                     FHKVGFEGGQMPLQRRLPKHGFVSPSRDDTAEVRLSDLNKLKTKDIDILALKEAGIVP
                     IAALSAKVMLSGKITHAVKLKGVLVSKGAKAAIEKAGGRVEAVETK"
     gene            2965..4272
                     /gene="secY"
                     /locus_tag="VLL03_02845"
     CDS             2965..4272
                     /gene="secY"
                     /locus_tag="VLL03_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013397001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HSS46336.1"
                     /translation="MFGMYSKMGDLKKRLLFLLGALVVYRVGTHIPVPGIDPVHLADL
                     FRSQQGGILGMFNVFSGGALSRFTIFALGILPYISSSIIMQLMTVVSPTLEALKKEGE
                     SGRRIITQYTRYGTVLLAMFQGMGIAIALEHQPGLVLEPGLMFRLTTMVTLVTGTMFL
                     MWLGEQITERGIGNGISLIIFAGIAAGLPHAVGGTLELTRTGAFAIPFVLVLFAAVTA
                     VTGLVVFVERGQRKILVNYAKRQVGRKIYGGQSSHLPLKLNMSGVIPPIFASSIILFP
                     ATLAGWFGSSENMTWLRDIGATLHPGQPIYVLLYAAAIIFFCFFYTALVFNPKETADN
                     LKKSGAFVPGIRPGEQTSRFIEHIMVRLTLAGATYVTLVCLLPEFLIVKWNVPFYFGG
                     TSLLIIVVVTMDFMAQVQAYIMSHQYESLLKKANFKGGGFPAR"
BASE COUNT          984 a         1203 c         1408 g          798 t
ORIGIN      
        1 cgacaagatc gacgcgctgc gcggtctcaa cattacgatc accaccaccg ccaagtccga
       61 cgacgaggca cgcgcgttgc tggcggcatt caaattcccg ttcaagaact gaggccggta
      121 tggcaaaaac ctccgtagtc aaccgtgaac tgaaacgccg cgccgtggtg aagaaattca
      181 ccgccaagcg cgcgggactt ctggctctca tcagcaacat gcgcttgagc gaggaggagc
      241 ggcatcaagc gcggctgaag ttgcagcagc tgccgcgaaa cagcagcccg gtaagattgc
      301 gcaaccgctg cgcactcacc ggccgcccgc gcggttacta ccgcaagttc ggcctggggc
      361 gcaacaagct gcgcgaaatc gccatgcgcg gcgagattcc cggcatcatc aaggccagtt
      421 ggtaacaggg agaaaccgat gagcatgacc gatcccatct ccgacatgct gacgcgcatc
      481 cgcaatgcgc agctttcgga aaaaaccact gtaatcctcc cctcgtcccg gctcaaggcg
      541 gccatcgccc aggtgctcaa ggatgagggc tacatcgagg gcttcgcggt gcgcgaagtg
      601 gaaggcaaga ccacgctgga aatcgcgctg aagtattacg ccggccgtcc agtgatagag
      661 aaaatcgagc gcgtcagccg cccgggcctg cgcatttatc gtggcagccg cgacatcccg
      721 aatgtgatga acggtttggg gatcgccatt atttccaccc ccaaaggggt gatgaccgac
      781 cgcaaggcac gagccaacgg cgtaggtggc gaagtgctgt gcatcgtcgc gtaacggaga
      841 ggaagccatg tcgcgaattg ccaagaatcc agtcgtcgtg ccggccggcg tcgaagtaac
      901 gcttagtccg agcgaagtcg cggtgaaagg gccgctgggc cagctcaaac agcacctcaa
      961 gggcgatgtg ctgatagaac gcgagggcga ccggctcaag ttcaaggtgg ggaaccagtc
     1021 caagcacgcc aatgtcatgt cgggcacgct gcgcgcgcat ctcgccaaca tgattcacgg
     1081 cgtcaccaag ggtttcgaga agaaacttag cttggtcgga gtgggttatc gcgcccaggc
     1141 gcagggcgac aagctcaatc tgtcgcttgg cttttcccat ccggtggtgc atcggatgcc
     1201 gaagggcatc aaggttgaaa cgccctcgct aaccgacatc gtcatcaagg gcatcgaccg
     1261 gcagctggtc ggccaggtaa cggcggaagt gcgcggctac cgcaagccgg agccgtacaa
     1321 aggcaagggc gtgcgctatg tcgatgaagt ggtgctgctc aaggaaacca agaagaaata
     1381 gacgagatca agcatggatg acaaaaaaca atcgcggctg cggcgctcgc gcaaaacccg
     1441 ggccaagatt cacgaactga ggatgatgcg cctcacggtg caccgtacca gcaaccatat
     1501 ttatgctcag gtcgtcaatg aaggcaaagt agtggcgact gcgtccacgc tggagcccga
     1561 ggtgcgcaag aaagtgaaat acggcggcaa cgtcaaggcg gcatcggtga ttggcaagcg
     1621 cattgcggaa aaagccagga agcaaggcat tgagtcggtg gccttcgacc gttccgggtt
     1681 caagtatcac ggccgggtga aagctctggc cgatgcggcg cgcgagaacg gcctgaaatt
     1741 ctgacggagc agaccatggc gaaaatgcag catcgttcgg aacagcggag cgacggcctc
     1801 agggaaaaaa tggtcgcggt gaaccgcgta accaaggtgg tcaaaggcgg ccgcatcctc
     1861 ggttttgccg cgctgacggt agtcggcgac ggcgacggcg gcgtcggcat gggcaagggc
     1921 aaggcgcgcg aagtgccggt cgcggtgcaa aaagcgatgg acgaggcgcg gcgcaaaatg
     1981 atcaaaatca ccttgaagaa cggcacgctg cagcatgcgg tgatcggcaa gcacggctcg
     2041 gcgaaagtgt acatgcagcc cgccatcgaa ggcacgggga ttatcgccgg cggtccgatg
     2101 cgcgcgattt tcgaggtgat gggagtgacc aatgtgctcg ctaagtgcct gggttcgacc
     2161 aatccctaca acgtggtgcg cgccaccctc cagggtttgc aggcgatgag cacgccttcg
     2221 gaaatcgccg ccaagcgcgg caagacagtg gaacagattc tggagtaaag catggtcagg
     2281 aaaaaagcgg caggcaaaat caaagcggcg ggcaaaatca aggtgaccct ggtgaaaagc
     2341 ccccaaagcg ccaaggaatc gcaccaggcc acggtgcgcg ggttgggttt gcgccgcctg
     2401 cgccataccg tcgagctcga agacaccccg gcggtgcgcg ggatgattaa caaggtcgga
     2461 tacctcctca agtgcgaggc ataaatggaa cttaacggca tcaaacccgc ggcaggcagc
     2521 aagcgcgaac ggcgccgcgt cgggcgtgga ccgggttcgg gcatgggcaa aaccgcgggc
     2581 cgcggccaca aaggccaaag agcgcgctcc ggcgggtttc acaaggtggg ctttgaaggc
     2641 gggcaaatgc cgctgcagcg gcggctgccc aagcacggct ttgtgtcgcc ttcccgcgac
     2701 gacaccgccg aagtgcggct ttccgatttg aacaaattga agactaaaga tatcgacatt
     2761 ctggcgctca aagaagcggg aatcgtgccg attgccgcgc tttccgcgaa agtcatgctc
     2821 tccggcaaga ttacccatgc agtgaaattg aaaggcgtgt tggtgagcaa aggcgccaaa
     2881 gccgctattg agaaagcggg cggcagagtc gaagcggtgg aaacgaaata acggaacaga
     2941 ggacacttgg ctactaacga ttcgatgttc ggcatgtaca gcaagatggg ggacttgaaa
     3001 aagcgcctct tgttcctgct gggcgccctc gtggtgtatc gcgtcggcac gcacattccg
     3061 gtacccggta ttgacccggt acatctggcc gacctgttcc gctcgcagca gggcggcatc
     3121 ctcggcatgt ttaacgtatt ttcgggcggg gcgctgtcgc gtttcaccat ttttgcgctg
     3181 gggatactgc cgtacatttc ctcctccatc atcatgcagt tgatgaccgt ggtgtcgccg
     3241 acgctcgagg cgctgaaaaa agagggtgaa tcggggcggc gcataattac gcaatacacc
     3301 cgctacggca cggtgctgct ggcgatgttc cagggcatgg gcatcgcgat tgcgctggag
     3361 catcaaccgg gcctggtgct ggagccggga ttgatgttca ggctcaccac catggtgacg
     3421 ctggtgaccg gcacgatgtt cctgatgtgg ctgggcgagc agatcaccga gcgcggcatc
     3481 ggcaacggca tttcgctgat tattttcgcc ggcatcgcgg cgggcctgcc gcacgcggtg
     3541 ggcggcacgc tggaactcac gcgcaccggc gcgtttgcga ttccattcgt gctggtgttg
     3601 ttcgcggccg tgactgcggt gacggggctg gtggtgtttg tggagcgcgg ccagcgcaag
     3661 attctggtga attacgccaa gcgccaggtg gggcgcaaga tttacggcgg gcaaagctcg
     3721 catttgccgc tgaagctcaa catgtcgggg gtgatcccgc cgattttcgc ctccagcatt
     3781 attttgtttc cggcgacact ggcaggctgg tttggctcca gcgagaacat gacgtggttg
     3841 cgcgatatcg gtgcgaccct gcacccgggg cagccgattt acgtgctgct gtatgcggcg
     3901 gcgatcatct tcttctgttt tttttacacc gcgctggtgt ttaatccgaa ggagaccgcc
     3961 gacaacctga agaaaagcgg ggcatttgtg ccgggcatcc ggccgggcga gcagacctcg
     4021 cgctttatcg agcacatcat ggtgcgcctg actttggcgg gcgcgactta cgtgactctg
     4081 gtgtgcctgc tgccggagtt tctgatcgtg aagtggaatg tgccgtttta tttcggcggc
     4141 acgtcgctct taattatagt ggtggtgacc atggatttta tggcgcaggt gcaggcctac
     4201 atcatgtccc atcaatacga gagcctgctg aagaaggcca atttcaaggg cggcggtttt
     4261 ccggcgcgct aatctgagac aagcagggat gattaaactc gggttgccta taaacaagta
     4321 ttttgcgtca aggcaaggcg caagtttttt tgctagcgcc gccgaagtat gtaagcgacg
     4381 caaaaaaacg aag
//
LOCUS       DATIOW010000090         4544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_447771, whole genome shotgun sequence.
ACCESSION   DATIOW010000090 DATIOW010000000
VERSION     DATIOW010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447771"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VLL03_02850"
     CDS             complement(<1..398)
                     /locus_tag="VLL03_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSS46337.1"
                     /translation="MLLKLTGGKIYDPANQINGEVRDLFVRDGKVAGVPDRDERIARE
                     FNLSGKIVMAGAIDLHTHIGGGKVNIARVMLPEDHRHDAVARTPLTRSGCGHAAPSTL
                     TTGYRYAEMGYTSCFEPAMLPANARQAHME"
     gene            complement(466..1734)
                     /locus_tag="VLL03_02855"
     CDS             complement(466..1734)
                     /locus_tag="VLL03_02855"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR03129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HSS46338.1"
                     /translation="MMQKTSSAVLDEVPCPFCELACDDLRIDARRLDVLANGCELSRA
                     SFALFNAPKTAAPRIAGKTATLAETAAEVARLLRPSRQPVFGGLGTDVAGMRALLHLA
                     DRCGAVVDHMDSAFLMRNVLALQDSGWMTTTLSEIKNRADLLILAGTDVVGRSPRFFE
                     RYVWNHESLFEHGKREIVYLGRGLNMQAGVSPDGREPEVIDCDVARIGELAGALRALV
                     AGKTLQAGKIAGVPPVELQKLASRMQKARYGVLVWSAADFDFPHAELAVQSLCELIKD
                     LNRATRFSGFPLGGNEGGMTASQVCAWQSGYPARIGFGSGHPEYDPHHFSAPRMLETG
                     ETDFLLWVSSFNFKRVPPASQAPTVVLGCNGMVFKKEPDVYIPVATPGIDHAGHIYRT
                     DNVVALRLRKLRETKLPSVADVITAIEKAI"
     gene            2041..3048
                     /locus_tag="VLL03_02860"
     CDS             2041..3048
                     /locus_tag="VLL03_02860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ribofuranosylaminobenzene 5'-phosphate
                     synthase family protein"
                     /protein_id="HSS46339.1"
                     /translation="MNGLLNADKEAQSVVVEAPARLHLGFMDVNGATGRRFGSLGLTL
                     DGFSTRIYAERAESFSVEGAQTQRAMSVLRCLQEKLGLRGAVRMAVVQAIPEHVGLGS
                     GTQLAIAAGVAVARLYQLNFGAREIAAVLGRGCRSGIGIGAFDQGGFLIDGGRGQGDE
                     PPRITSRIAFPPQWRILLVFDLQAQGLHGAREADAFRTLPSFPEASAAHLCRLVLMHV
                     MPALVEVNLQEFGKAVTEVQQVVGDHFAPVQGGRYASRKVSEVLAWLEMQGVACLGQS
                     SWGPTGFAVVDSEMRAAALMREAEARWGQERQLRFRVCAARNHGGEVLLQRCGVKTGT
                     A"
     gene            3102..3980
                     /locus_tag="VLL03_02865"
     CDS             3102..3980
                     /locus_tag="VLL03_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent
                     methylenetetrahydromethanopterin dehydrogenase"
                     /protein_id="HSS46340.1"
                     /translation="MFTPTPNVSPFDVNMAYDAGYNAVMAYSNVMLDQVAALTQDAIF
                     SRGPTGVKRTGIFIGGREIGMAADMLDAARNAMVPPFEVSVFADPSGAFTTAAAMVAA
                     VERQLKKTHRRGLKGCEVLVLGGTGPVGMAAAVLASGAGAKVRIASHSSAIRARVSAE
                     TANARYGAKTEPADASSEQGKMAVIPNVNVVLATAKAGVRVLSQKELAHAKKLLVAAD
                     VNAVPPAGIEGVGVMDDGVPFAAGSGKAVGIGALAIGDVKYQVQRKLFEAMLNTGKPL
                     YLDFRDAFALARKHVS"
     gene            3970..>4544
                     /locus_tag="VLL03_02870"
     CDS             3970..>4544
                     /locus_tag="VLL03_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46341.1"
                     /translation="MSAEKAYLIAALTGRALAQSASRAQLPVVVLDLYNDVDTRRVAT
                     RARAVVAHNESFDAKALLKAAEELCPVPACAGLIEGSGFEDRPELLRQLAHGRRLYGN
                     TPGLVALLKDPARFFPLLNRLAIPHPEVSLQPPRKARGWLSKKTGGNGGGHVLLAAGT
                     VADAQHYYQRLESGRSMSVTFLANGRETHIIG"
BASE COUNT          885 a         1295 c         1472 g          892 t
ORIGIN      
        1 atttccatgt gcgcctggcg cgcattggcc ggcagcatgg ccggctcgaa gcacgaggtg
       61 tagcccattt cagcgtagcg gtagccggtg gtgagtgtgg acggcgcggc atgaccgcag
      121 ccggagcggg tgagcggtgt gcgcgcaacg gcatcgtggc gatggtcctc cggcaacatg
      181 acgcgcgcga tgttgacctt gccgccgccg atgtgggtat gcaggtcaat ggcgccggcc
      241 atgacgattt ttccggaaag attgaattcg cgggctatgc gttcatcgcg gtccggcact
      301 ccggccacct tgccgtcacg cacaaacaga tcgcgcacct cgccgtttat ttggttggcc
      361 gggtcgtaga tttttccgcc ggtaagtttt agtaacatat gtttgaaccg cgggggcgca
      421 gagccgcaga gaaattgctt cctctgcgcc tcagcgtctc tgcggttaga tcgctttttc
      481 aatagctgtt atcacgtctg ccacactcgg caattttgtt tcgcgcagct tgcgcaagcg
      541 cagcgccacc acgttgtcgg tgcggtagat gtgcccggca tgatctatcc ccggtgtcgc
      601 caccggaatg taaacatccg gttctttttt aaagaccatg ccgttgcagc ccaggactac
      661 ggtgggcgcc tggcttgcgg gcggaacccg tttgaagttg aaactggaaa cccagagcag
      721 aaaatccgtt tcgcccgttt ccagcatgcg cggagccgaa aaatggtgcg gatcatattc
      781 aggatggccg ctcccgaaac cgatgcgcgc cggatagccg ctttgccagg cacagacctg
      841 gctggcggtc atgccgcctt cattgccgcc cagcggaaag cccgagaaac gggtggcgcg
      901 gttcaaatcc ttgatgagtt cgcacaggct ttgcaccgcc agctcggcgt ggggaaaatc
      961 aaaatcggca gccgaccata ccagcacgcc gtaacgcgct ttttgcatgc gcgaggccag
     1021 cttttgcagt tccaccggcg gcacgcccgc gattttgcca gcctgcaggg ttttaccggc
     1081 aaccagcgcg cgcagcgcgc cggcgagttc gccgatgcgt gcaacatcac aatcaataac
     1141 ttcgggttcg cgcccgtcgg gagaaacgcc cgcttgcatg ttgagcccgc ggccgagata
     1201 gacgatttcg cgctttccat gctcgaacaa tgactcgtgg ttccagacgt agcgctcaaa
     1261 aaagcgcggg gagcggccga ctacgtcggt gccggccagt atcagcaaat cggcgcggtt
     1321 ttttatttcg ctcaaggtcg tagtcatcca accgctgtcc tgcagcgcca ggacgttgcg
     1381 catcaagaaa gcgctgtcca tgtggtccac caccgcgccg cagcggtcgg cgagatgaag
     1441 caatgcgcgc attcccgcga catcggtgcc caaaccgccg aataccggct gccgtgacgg
     1501 gcgcagcagc cgcgcaacct cggcggcggt ttcagccaac gtagcggtct ttcccgcgat
     1561 ccgcggagcg gcggtcttgg gcgcattgaa taaagcaaaa cttgcgcgtg aaagctcgca
     1621 gccgttggcc aaaacgtcca gccgcctcgc atcgatgcgc aggtcgtcgc aagcgagctc
     1681 gcaaaacggg cacggcactt cgtccagcac ggctgaagag gttttttgca tcatcgcagg
     1741 attgagtaaa tgatcattgg gaacccacag gcacatacta tgccatcgcg cgcgctttat
     1801 gggtaggcgc ccctggctgc ggtgatttgt ccgctacaca agggattttt gctatatatt
     1861 aagcatatac gttcaaggcg cggaacattg cagcgcatca cgctaggcac tgcgaaagtg
     1921 acatactgct ccgcgcgcgt cgcaaccaac ccctcgggcc gccgcgaccg gcttgaaata
     1981 caatgaaaaa accgaatggc atgatagttg ctgaattttc cagactgaaa tcacaagata
     2041 atgaacggct tgctgaacgc cgataaagaa gcccagagcg tagtggtgga agccccggcg
     2101 cgcttgcact tgggtttcat ggatgtgaat ggtgcaaccg gccggcgctt cggcagcttg
     2161 gggctcacgc ttgacggttt cagcacccgc atctacgccg agcgcgcgga aagtttttcg
     2221 gtagaaggcg cgcaaacaca gcgcgccatg agcgtactac gttgcctgca ggaaaagctg
     2281 ggtttgcgcg gcgcggtgcg catggcggtg gtgcaggcga ttcccgaaca tgtgggtttg
     2341 ggttcgggca cgcagcttgc gattgccgcc ggcgttgccg tggcgcggct ttaccagctt
     2401 aacttcggcg cgcgggaaat cgcggcggtg ctgggccgcg gctgccgctc tggcatcggc
     2461 atcggcgcgt ttgaccaggg cggttttttg attgacggcg gccgcggcca aggcgacgag
     2521 ccaccgcgga tcaccagccg catcgcgttt ccgccgcagt ggcgcatcct gctggttttc
     2581 gatttgcagg ctcagggatt gcatggcgcc agggaagccg atgcttttcg cacattgccg
     2641 tcgtttccgg aagccagtgc ggcgcacttg tgccggctgg tgctgatgca tgtcatgccg
     2701 gcattggtgg aggttaatct acaggaattc ggcaaggctg tgaccgaagt gcagcaagtg
     2761 gtgggcgacc atttcgcacc ggtgcagggc ggccgctatg cgagccgcaa ggtgagcgag
     2821 gtgctggcct ggctggaaat gcagggtgtc gcttgcttgg gccagagctc atgggggccg
     2881 accggttttg cggtggtgga ttcggaaatg cgcgcggcgg cgctcatgcg cgaggcggaa
     2941 gcacgctggg gccaggaacg ccagctgcgc tttcgtgttt gcgcagcgcg caaccatggg
     3001 ggtgaagtgt tgctgcaacg gtgcggcgtt aagaccggca cagcttaaaa aataattcaa
     3061 taattctagg ggaggctatg cagaaaccgc aattattaca catgttcaca cctactccga
     3121 atgtaagtcc gtttgacgtc aacatggcgt atgacgcggg ttataacgcg gtaatggctt
     3181 acagcaatgt catgcttgac caagtcgcgg cgctgacgca ggacgcgatt ttctcgcgcg
     3241 gtcccacggg tgtgaagcgc accggcattt tcattggcgg gcgcgagatc ggcatggcgg
     3301 cggacatgct ggatgcggcg aggaatgcca tggtgccgcc gttcgaagtg tcggtttttg
     3361 ccgacccttc cggcgctttt accaccgcgg cggcgatggt ggcggcggtg gaaagacaat
     3421 tgaaaaaaac gcatcgccgc gggctgaaag gttgcgaagt gctggtgctg ggcggcaccg
     3481 ggccggtggg catggcggcg gcagtgctcg cctccggcgc cggcgctaag gtgcgcatcg
     3541 ccagtcactc gagcgcgatc cgggcacgtg tgtccgcgga aacggcgaat gcgcgctacg
     3601 gcgcgaagac tgaaccggcg gatgcgagct ctgaacaagg caagatggcc gttatcccca
     3661 acgtcaacgt ggtgcttgcc accgccaagg cgggcgtgcg ggtgttgagc cagaaggaat
     3721 tggcgcacgc caagaagctt ttggtggcgg cggatgtcaa cgccgtgccg cccgccggaa
     3781 tcgaaggcgt gggcgtgatg gacgacggcg tgccgtttgc cgcgggctcc ggtaaagcgg
     3841 tcggcatcgg cgcactcgcc atcggcgatg tgaagtacca ggtgcaacgc aagttattcg
     3901 aggcgatgct caacacggga aaacctctct atctggattt ccgcgatgca tttgctttgg
     3961 cgcgaaaaca tgtcagctga aaaagcgtat ttgatcgccg cattaaccgg ccgggcgctg
     4021 gcgcaatccg caagccgcgc tcagttgccg gtagtagtgc tcgacttgta taacgatgtg
     4081 gacacacggc gcgtagcaac acgcgcccgg gcggtagtcg cgcataacga aagcttcgat
     4141 gccaaggcct tgctcaaagc cgcggaagag ttatgccccg ttccagcctg cgccgggctc
     4201 attgaaggct cgggttttga agaccgcccg gaactgttgc ggcagctggc acacggacgg
     4261 cgcctgtatg gcaacacacc gggactagtg gcgctgctca aagaccccgc gcggtttttc
     4321 ccgctgctca atcggctggc gattccgcat ccggaagtga gcctgcagcc gccgcgcaag
     4381 gcgcgaggat ggctttccaa gaaaaccggg ggcaacggcg gcggtcatgt gctgctcgcg
     4441 gcgggcacgg tggcggatgc gcaacattat taccagcgtc ttgaatccgg tcgcagcatg
     4501 tcggtgactt ttctcgccaa cggcagagaa acgcacatta tcgg
//
LOCUS       DATIOW010000091         1985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_347903, whole genome shotgun sequence.
ACCESSION   DATIOW010000091 DATIOW010000000
VERSION     DATIOW010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347903"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..1121
                     /locus_tag="VLL03_02875"
     CDS             249..1121
                     /locus_tag="VLL03_02875"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002306.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HSS46342.1"
                     /translation="MNNPFKTIALIGKYKSPEIAGHLQRLARFLTERGATVLVDRLTA
                     VHLENSAFPVLALEEIGKRANLAIVIGGDGTMLNIARTFAPHNVALVGVNQGRLGFLT
                     DISADTMLETIGAILDGKYVTEERMLLSAEILSNNQSVLKVLVFNDVVVSKGAKGSMI
                     EFDIHINQQYVYTLRSDGLIVATPTGSTAYALSSGGPIVHPSLSLIALVPVSPHTLSN
                     RPIIVSGDSTVEILMHDSGDARAHFDSHSHFDLRDGDKIQVRRAPDTIRLLHPVGHNY
                     YHMLREKLHWSKTT"
     gene            1124..>1985
                     /locus_tag="VLL03_02880"
     CDS             1124..>1985
                     /locus_tag="VLL03_02880"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSS46343.1"
                     /translation="MLQTLSIRDFVIVDTMELEFKPGFTVLTGETGAGKSILIDALAL
                     TLGERADGVVVRNGAERAEIAAEFNIGKLTPLSAWLRENDLEGDPGACMMRRVIEANG
                     RSRGFINGHTATVQQLREAGEKLVEIHGQHAHQTLLKAESQRELVDAYGGLGELATRV
                     AEYYRQWQSLRKRRQEWEANAAAFTAEREQWEWQARELKTLNFSSDEWQTLQADHSRL
                     AHAATLLETAGMALDTLSENETALIAQLSSQISRFRNLIEHDPGLKEILDILEPAQIQ
                     LQEAVYSIRHY"
BASE COUNT          434 a          568 c          577 g          406 t
ORIGIN      
        1 tgcgctcgcg gaccgtcgcg cctcggaacg tgcgcagggg agcgagcgca gcgccgcctc
       61 caggtcgcac tgccgaggct ccctctgcgg cggcgctatc ccggaaagcc gccgtcgcaa
      121 tcgtgtggga cttaatcaga ggttccttac cattcagcta tcagcttgaa tctggcgcgg
      181 ctgcgcagtg agttctgccc actgagggct gatagctgat ggctgcaaaa tatgatttaa
      241 tgccctgcat gaacaatcct tttaaaacca ttgccctcat cggcaaatac aaaagtccgg
      301 aaatcgccgg gcatctgcag cgcctggcgc gcttcctgac ggaacgcggc gccacggtgc
      361 tggtggacag actgaccgcg gtgcatctcg aaaacagcgc ttttccggta ttggctctcg
      421 aggaaatcgg caagcgcgcc aatctcgcca ttgtaatcgg cggcgacggt accatgctca
      481 atatcgcccg cacctttgcc ccccacaacg tggccctggt gggcgtcaat cagggacggc
      541 tggggtttct taccgacatt tcggcagaca ccatgctgga gaccatcggc gccatactcg
      601 acggtaaata tgtcactgaa gaacgcatgc tgctttccgc agaaatcctg agcaacaacc
      661 aaagtgtgct caaggtgctg gttttcaacg atgtggtggt gagcaaaggc gccaagggca
      721 gcatgattga atttgacatt catatcaatc aacaatacgt ctataccttg cgttcagacg
      781 gcctgattgt cgctactccc accggctcca ctgcttacgc gctgtcatcc ggcgggccta
      841 tcgtgcatcc ttcgctgagc ctgatcgccc tggtgccggt ttccccgcat accttgagca
      901 accgcccgat catagtaagc ggcgattcca ccgtggaaat cctgatgcac gattccggtg
      961 acgcgcgtgc gcacttcgac agccattcgc attttgattt gcgagatggc gataaaatac
     1021 aggtgcggcg ggctcccgat accatccgac tgttgcaccc ggtggggcat aattattacc
     1081 acatgctgcg cgaaaagctg cattggagca aaacaacgta accatgttac agactctgag
     1141 catccgcgat tttgtcatcg tcgacaccat ggagctcgaa ttcaagccgg gctttaccgt
     1201 gctgacgggg gagaccggcg cgggaaaatc catactgatt gatgcgctgg cgctcaccct
     1261 gggcgagcgc gctgacggcg tggtggtgcg taatggcgca gagcgcgccg agatcgccgc
     1321 ggagtttaat atcggcaaac taaccccgct ttccgcctgg ctgcgtgaaa atgatctgga
     1381 gggtgatccc ggcgcgtgta tgatgcgccg tgtgattgaa gccaacggcc gctcgcgcgg
     1441 attcatcaac ggccatacgg caaccgtgca gcagctgcgc gaagcaggcg agaagctggt
     1501 agaaatccac ggccagcatg cgcaccagac gctgctcaaa gccgagtcgc agcgtgaact
     1561 ggtggatgcc tatggcggat taggagagct ggccacgcga gtcgctgagt attaccgtca
     1621 atggcaaagc ttgcgcaaac ggcggcagga atgggaggct aacgccgccg cctttaccgc
     1681 cgagcgcgag cagtgggaat ggcaggcgcg cgagctgaag accctgaatt tttcctccga
     1741 tgagtggcaa acgcttcagg cggatcactc gcgtcttgca catgccgcaa ctttgctgga
     1801 gactgcggga atggcgcttg atacgctttc ggaaaacgag actgcactga tagcacaact
     1861 tagttcgcag atttcccgat tccggaacct gatcgaacat gaccccggtt tgaaggaaat
     1921 tctggatatt ctggagccgg cgcaaatcca gttgcaggaa gcggtgtact cgattcggca
     1981 ttacc
//
LOCUS       DATIOW010000092         2807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1669633, whole genome shotgun sequence.
ACCESSION   DATIOW010000092 DATIOW010000000
VERSION     DATIOW010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669633"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VLL03_02885"
     CDS             complement(<1..681)
                     /locus_tag="VLL03_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSS46344.1"
                     /translation="MQRYFTNTSSSGNPPQVDIIPVEAAGTLGGLFRERVRRTPDGVA
                     YRDFNLKHGNWRDYTWAQMQRQITRWQAALKREQLDPGDRVALMLRNCPTWVTMDQAS
                     LGLGLVVVPLYTVDRPDNVAYILKDAGVKVLLLENQEQWKQLEAVREQWQDCGLQRIL
                     VLNGVDAADRNDARLAAVEDWLPEAGAEEMETLGKTDELATIVYTSGTTGRPKGVMLS
                     HRNILTNVF"
     gene            complement(687..1505)
                     /locus_tag="VLL03_02890"
     CDS             complement(687..1505)
                     /locus_tag="VLL03_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSS46345.1"
                     /translation="MSLKGKTLFITGASRGIGREIALRCARDGANIVIAAKTAAPHPK
                     LSGTIYSVAEEVKTAGGQALPIQLDVREEAQIMAAAAKAAETFGGIDILVNNASAISL
                     TDTLHTPMKRFDLMMSVNVRGTFLSSQACIPHLRRAVNPHILNLAPPLDMRAKWFKDH
                     VAYTMSKYGMSLCTLGMSAELAQDGIAVNSLWPRTAVATAAVKVHFPEEVFKASRKPA
                     IVADAAYVIFNCDSRSTTGNFFIDEEVLRGAGVSDFSRYAVTPGAKLVNDFFVE"
     gene            complement(1514..2299)
                     /locus_tag="VLL03_02895"
     CDS             complement(1514..2299)
                     /locus_tag="VLL03_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HSS46346.1"
                     /translation="MTQSILLSVADPIATITFNRPKVMNALDWDMIVRFREVCEEVRR
                     AEEVRAVLMRGEGPAFLAGGDVAMFRANLKQLPELIVKGARELHNAMLALRRMNKPVV
                     ASVHGAVAGGGMSVMLSADLVIAADNTQFTMAYSRIATSPDGGSTYFLPRMVGYHKAM
                     ELALLSDTIDAETAKSLGIVNFVVPAAELQAQTAKLMQRLAQGATYAYGETKVLMNQS
                     INASMEEQLEAEVQAFSRCARTADLAEGVTAFVEKRKPAFTGK"
     gene            complement(2296..>2807)
                     /locus_tag="VLL03_02900"
     CDS             complement(2296..>2807)
                     /locus_tag="VLL03_02900"
                     /inference="COORDINATES: protein motif:HMM:NF020876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase domain-containing
                     protein"
                     /protein_id="HSS46347.1"
                     /translation="WRTQLALEGILQNFPLMTWILWRLVFPLGRSFIPPSDDLGHAVA
                     SLLLAPSKVRDRLTAGIYVPADETDPVGRLDIALQAAIAAEAVEAKIRVGAKAGHIKG
                     KTPEELAAQAAQQGIISVEEADMLAKAKQLRRAVIMVDDFPQDFGKSEWAGSSLAQQE
                     MSSTSRRTA"
BASE COUNT          519 a          893 c          802 g          593 t
ORIGIN      
        1 gaagacgttg gtaagaatat tgcggtgcga gagcatcacg cccttgggcc ggccggtggt
       61 accggacgta tagacaatgg tggccagctc gtcggttttc cccagcgttt ccatttcctc
      121 cgcgcctgct tcgggaagcc agtcttctac cgcggcgagg cgcgcatcat ttctgtcagc
      181 ggcatcgacg ccattcagca ccagaatgcg ctgcaagccg cagtcttgcc actgctcgcg
      241 cacagcctca agctgtttcc attgctcctg gttttccagg agcagcactt tgactccggc
      301 atctttcagg atataagcca cgttgtccgg ccggtcaacg gtataaagcg gcaccaccac
      361 cagtcccaaa cccagggatg cctggtccat cgtcacccat gtcgggcagt tgcgcagcat
      421 cagcgccacc cgatcgccgg gatcaagttg ttcgcgtttc agcgccgcct gccagcgcgt
      481 aatctgtctt tgcatctgcg cccaggtgta atcgcgccag ttaccgtgtt tcaggttgaa
      541 atcgcgatag gccacgccgt ccggtgtacg tctgacgcgc tcgcggaaaa gcccgcccag
      601 ggttcccgcc gcttcaaccg ggatgatgtc aacctgcggc gggtttccgc ttgagcttgt
      661 gttggtaaaa taccgctgca tgaggctcat tccacaaaaa aatcattcac cagtttcgcg
      721 cccggcgtca ccgcgtagcg ggaaaaatcg ctcacgccgg caccccgtaa aacctcttcg
      781 tcaatgaaaa aattgccggt agtcgaacgg ctgtcgcagt tgaaaatcac gtaagccgcg
      841 tccgccacga ttgccggctt gcggctcgcc ttgaaaactt cttcagggaa atgtacttta
      901 accgccgcag tggcaaccgc cgtgcgcggc caaagcgaat tcaccgcgat accatcctgc
      961 gccagttccg ccgacatccc cagcgtgcac aagctcatac cgtacttcga catggtgtag
     1021 gcgacatggt ccttgaacca cttggcgcgc atatccagcg gcggtgcaag attaagaata
     1081 tgcggattga cggcgcgcct gagatgggga atgcacgcct gcgagctcaa gaacgtgccg
     1141 cgcacgttga ccgacatcat caaatcaaag cgcttcattg gcgtgtgcag cgtatcggtg
     1201 agactgatgg cgctggcatt gttgaccagg atgtcgatgc cgccgaaggt ttcagccgct
     1261 ttcgccgccg ccgccatgat ttgcgcttcc tcgcgcacgt cgagctgtat cggcagcgcc
     1321 tggccccctg ccgtcttcac ttcctcggca acgctgtaaa tggtgccgga aagcttcgga
     1381 tgcggcgcgg cagtcttagc ggcaatcaca atgttcgccc cgtcgcgcgc gcagcgcagc
     1441 gcgatttcac ggccgatgcc gcgcgaggcg ccggtgatga acagggtttt gcctttgagc
     1501 gacatgcgac tgcctatttg ccggtgaacg ccggcttgcg tttttcaaca aacgctgtga
     1561 cgccctcggc cagatcggcg gtgcgggcgc agcgggagaa cgcctgcact tccgcttcca
     1621 attgttcttc catgctcgcg ttaatcgatt ggttcatcag caccttggtt tcgccataag
     1681 cataggttgc accctgggcc aggcgctgca tgagcttggc cgtttgggct tgcaactcgg
     1741 cggccggtac cacgaaattc acgatgccga gactcttcgc ggtttcagcg tctatcgtat
     1801 cggagagcag cgccagttcc atcgccttgt gatagcccac catgcgcggc aggaaataag
     1861 tgctgccgcc gtcggggctg gtggcgatgc ggctgtaggc catggtgaat tgggtgttat
     1921 ccgccgcgat taccagatcc gcggaaagca tgacactcat gccgccgccc gccaccgcgc
     1981 cgtgcacgct ggccaccacc ggcttgttca tgcgccgcag cgccagcatc gcgttgtgca
     2041 gttcgcgcgc gcctttcaca atcagttccg gcaattgctt gaggttggcg cggaacatgg
     2101 cgacatcgcc gccggcgagg aacgccggcc cctcaccgcg catgagcacc gcgcgcacct
     2161 cctctgcgcg ccgcacttct tcgcacacct cgcgaaaacg cacgatcatg tcccagtcga
     2221 gcgcgttcat caccttgggg cggttgaaag taatggtcgc aatagggtcg gcgaccgaga
     2281 gcagaatgct ttgcgtcacg cggttctcct tgacgtggac gacatttcct gttgcgccag
     2341 gctgcttccc gcccattcgc ttttgccgaa gtcctgggga aaatcgtcca ccatgatcac
     2401 cgcgcggcgg agttgttttg ccttggcgag catgtccgcc tcttccacgc tgatgatgcc
     2461 ctgctgcgct gcttgcgcgg cgagctcttc cggcgttttg cctttgatgt gcccggcttt
     2521 tgctccgacg cggatcttcg cttcaaccgc ttccgccgca atcgccgcct ggagggcaat
     2581 atccagacgg ccgaccggat ccgtttcatc ggccggcaca taaataccgg cggtcagccg
     2641 gtcccggact ttggaaggcg cgagcaggag gctggctact gcatgcccca aatcatcgct
     2701 cggcgggatg aaacttctgc ccagcggaaa caccaaccgc cacaatatcc atgtcatcag
     2761 tggaaagttc tggagtatgc cctccagcgc cagttgcgtg cgccaca
//
LOCUS       DATIOW010000093         5492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1162150, whole genome shotgun sequence.
ACCESSION   DATIOW010000093 DATIOW010000000
VERSION     DATIOW010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162150"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VLL03_02905"
     CDS             complement(<1..823)
                     /locus_tag="VLL03_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HSS46348.1"
                     /translation="MKYQDLRDFIAQLEKLGELKRVAAEVDPRLEMTEICDRVLKSQG
                     PAILFEKPKGYSIPVLGNLFGTVKRVALGMSGESVEALRKVGKLLAYLKEPDPPQGLK
                     DAWDKLPVLKQVLNMAPKEVSGAPCQEIVWEGGSVDLSRLPIQTCWPEDAGPQITWGL
                     TVTRGPHKKRQNLGIYRQQAIAPNKVIMRWLSARGGALDFRDHTLAHPGTPFPVAVAL
                     GADPATLLGAVTPVPDSLSEYQFAGLLRGAKTEVVKCLSHDLQVPASAEIVLEGAI"
     gene            complement(834..1709)
                     /gene="ubiA"
                     /locus_tag="VLL03_02910"
     CDS             complement(834..1709)
                     /gene="ubiA"
                     /locus_tag="VLL03_02910"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HSS46349.1"
                     /translation="MFKERLNLYEKLMRLDKPIGILLLLWPTLWGLWISSYGRPNWAI
                     LWIFVMGAVLMRSAGCVINDYADRDFDKQVERTMNRPLASGQVSTGEALLLAAVLAFF
                     AFLLILPLDHLVILLSVPALLIAATYPFTKRFFAIPQAYLGIAFGFGIPMAYAANVGA
                     VPPIAWVLLLANMFWSIAYDTEYAMVDRDDDIRIGIKTSAITFGKYDVLVVMLCYTAM
                     LVILAGIGLRFKFGIFYYAGLLTAAGIMGYHYTLIKNRERDKCFKAFLHNNWVGAAIF
                     AGIALDLYFRFRNHF"
     gene            complement(1709..2161)
                     /locus_tag="VLL03_02915"
                     /pseudo
     CDS             complement(1709..2161)
                     /locus_tag="VLL03_02915"
                     /inference="COORDINATES: protein motif:HMM:NF016248.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
     gene            complement(2322..4418)
                     /gene="recG"
                     /locus_tag="VLL03_02920"
     CDS             complement(2322..4418)
                     /gene="recG"
                     /locus_tag="VLL03_02920"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006408353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HSS46350.1"
                     /translation="MRGEKQKGGSAYSSKKPGTPHAITGISKSTQDKLAKLGIATPLD
                     LVLHLPLRYEDETRLYPIRDAPTGQTVQVEGVVVNTEIQYRPRRTLVCQVEDGTGPLV
                     MRFFNFYGSQVKALAPGARVRVLGEIRQGFFGAEMVHPKYRSVRGDMPLPDSLTPVYP
                     TTAGLSQDTLRQLIKHSLAENDLAESLPRQILQAFKLPGFRESVLFLHQPPPRVKLPQ
                     LVQRGHPAWRRIKFDELLAQQLSMRLHYRERRSRVAMALPKRGRLTRALHQGLPFVLT
                     AAQRRAMEEIAGDLSQPYPMQRLLQGDVGSGKTIVAAFAVLQVIESGCQAAIMAPTEI
                     LAEQHYHKFSGWLAPLGIRVAWLAGSLSKKQKQVALKSMLQSEATLVIGTHALIQEQV
                     EFRKLGLAIIDEQHRFGVHQRLALRIKGSRPGLQPHQLMMSATPIPRTLSMSYYADLD
                     VSVIDELPPGRAPVTTKLVADSRRAEVVARVRDACVAGKQAYWVCPLIEESEALQLKT
                     ALETYSVLKSTFPELQVGLVHGRLASQEKAAVMQAFQQGEIQLLVATTVIEVGVDVPN
                     ASLMVIEHAERMGLSQLHQLRGRVGRGEQNSVCILMYQKPLGEAARERLKIVFETADG
                     FEIARQDLLLRGPGEFLGARQSGVPLLRFADLNSDSDLLEAARNAAARMLDEFPQAVN
                     AHLARWLGGRKDYLRV"
     gene            complement(4436..4819)
                     /locus_tag="VLL03_02925"
     CDS             complement(4436..4819)
                     /locus_tag="VLL03_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016803893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSS46351.1"
                     /translation="MAKKIIQTNAAPKAIGTYSQAVRAGQTVYLSGQIGLDPASMQLV
                     EGIEAQIVRVFENLKAVAQAAGGGLDNVVKLTVFLTDLGHFARVNEIMAKYFHEPYPA
                     RAAVGVAALPRGALVEMDAVLVLSE"
     gene            4857..5342
                     /locus_tag="VLL03_02930"
     CDS             4857..5342
                     /locus_tag="VLL03_02930"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS46352.1"
                     /translation="MPGVFSAKNSRHVIRFARFRHRFCLTECPDCAIITAFANVNSSP
                     TVSPLIANLEKMLAAGRDDALLRFSLGNEYLKNSEFAAAAQHLGEAVRHDPLYSAAWK
                     LLGKAFAECGRLEDALNAYQRGIEVAEKKGDKQAAKEMQVFARRIEKQLAQQSESGQQ
                     Q"
BASE COUNT         1184 a         1682 c         1506 g         1120 t
ORIGIN      
        1 gaatcgcgcc ttccaaaacg atttcggcgc ttgccggcac ctgcaaatcg tggcttaagc
       61 atttcaccac ttcggttttg gcaccgcgca acagccccgc aaactgatat tcactcaagc
      121 tgtccggcac cggcgtcacc gcgccgagca gggtcgcggg gtccgcgccc aacgccacgg
      181 ccaccgggaa aggcgtcccg gggtgcgcga gcgtgtggtc gcgaaaatcc agcgcgccgc
      241 ctcttgcgct aagccagcgc atgatcactt tattgggcgc aatcgcctgc tggcggtaaa
      301 tacccaggtt ctgccgcttc ttgtgcggcc ctcgggtaac ggtcagcccc caggtgatct
      361 gcggcccggc gtcttccggc cagcaggttt ggatcggcag gcgcgataaa tctacgctcc
      421 cgccttccca cacgatttcc tggcagggcg cgccggaaac ttctttcggc gccatgttga
      481 gcacttgttt cagcaccggt agtttatccc aggcatcttt caagccttgc ggcgggtcgg
      541 gctccttgag ataggcaagg agctttccaa ccttacgcag cgcttcgact gactccccgc
      601 tcatgccgag ggccacgcgc ttaaccgtgc cgaataaatt gcccagcacc ggaatgctgt
      661 agcccttggg tttttcaaac agaatcgccg gtccttgcga tttcagaacg cggtcgcaga
      721 tttcggtcat ttccagccgc ggatcgactt cagcggcgac gcgcttgagc tcgcccagct
      781 tttccagttg cgcgatgaag tcgcggaggt cctggtattt catttaatcc cgttcagaaa
      841 tggttacgga aacgaaagta aaggtcgagc gcgatgccgg caaaaatcgc cgcgcccacc
      901 cagttgttat gcagaaacgc cttgaagcat ttgtcgcgct cgcggttctt aatcaaggtg
      961 tagtgatagc ccattatgcc tgcggccgta agcagcccgg cgtaataaaa tattccaaat
     1021 ttgaaccgca ggccgatacc ggcaagtatc acaagcatgg cggtataaca cagcattacc
     1081 accagcacat cgtattttcc gaaagtgatg gccgaagttt taatcccgat cctgatgtca
     1141 tcgtcgcggt ccaccatggc gtattcggtg tcgtaggcaa ttgaccagaa catattcgca
     1201 aggagcagca cccaggcaat cggaggaacc gcgccaacat tcgcggcata ggccatgggg
     1261 atgccgaagc cgaaagcgat gcccagataa gcttgtggaa tagcaaaaaa tcttttggtg
     1321 aaaggatagg tcgcggcgat cagcagcgcc ggtaccgaaa gcagaattac cagatggtcg
     1381 agcgggagaa tcagcaaaaa tgcaaaaaac gccagcaccg cggccagcag cagcgcttct
     1441 ccagtactga cttggccgct ggcaagcggc cggttcatgg tgcgctcgac ttgcttgtcg
     1501 aaatcgcggt cggcgtagtc attgatgaca cagccggctg accgcatcaa taccgcaccc
     1561 ataacaaaaa tccaaagtat tgcccagttc gggcggccgt aagaggaaat ccacagaccc
     1621 cacagcgtcg gccacagtaa aagcagaatg ccgatgggct tatcgagccg catcagcttt
     1681 tcatagaggt tgagccgctc cttgaacatc atcgcgaaag ttccaaaatt ccgggaagaa
     1741 atatttcagt caccattacc ggcctgctac gcaaaataaa gaccgagcgc cttgcccaca
     1801 agtagttcgg cggcgttctc agcaaccggc atgccctgtg ataaagctca tgatgcgcag
     1861 tgagcttttt aaaatacagg ggcgtgcgct tgaccagcgg attggcgaac aatgctgcgc
     1921 ccaagggttt caggccctgc cggctgatgg agcgccagac cccgcgcaga ctccgcctgc
     1981 cgagcacgct gtgcgcaaac accaccgggg tttcgccgca ataaagatta acttcgcgca
     2041 acatgaccag ctcgccgcgc cgcgcgccca ccaaggctga ttcatcgcgg cagggctgcg
     2101 ccagtttttg ccacacatgg cgcacgctga acgcgctgca acgcatttgg atacgctggg
     2161 tgagcgagcc cgcgcgaagt caaccaggaa tgataagaac gcggcggatt ggtcgggtgc
     2221 cgtcgccatg cctgtttcac ctgtttacag agtgtgtgaa gaagaccact tattatgcca
     2281 ccaagaacct ctgataccgc gaaggactta atcagaggtt cctacactcg aagatagtct
     2341 ttcctgccgc ccagccagcg cgcgaggtgc gcgttcaccg cttgcggaaa ttcgtcgagc
     2401 atgcgagcgg cggcgttgcg cgccgcttcc agcaaatcgg agtcactgtt gaggtcggca
     2461 aaccgcaaca gcggtactcc actctgccgt gcgccgagaa actcccccgg cccgcgcagc
     2521 agcaaatcct gtctggcgat ttcaaagcca tcagccgtct cgaacacgat tttgagccgc
     2581 tcccgcgccg cttcgcccag cggcttttgg tacatcagaa tgcacacgct gttctgctcg
     2641 ccgcgcccga ctcgcccacg caactgatgc agttgtgaca gccccatgcg ctcggcatgc
     2701 tcgatgacca tcaacgaagc attgggcacg tccacgccga cttcgatcac cgtggttgcc
     2761 accagcaatt gaatttcacc ctgttgaaac gcctgcatca ccgccgcttt ttcttggctg
     2821 gcaagcctgc cgtgcaccag ccccacttgc aattcgggaa acgtgctctt taacacggaa
     2881 taagtctcca gcgcggtctt gagttgcagc gcctcggatt cttcgatcag cgggcacacc
     2941 caatacgcct gcttgcccgc tacacaggca tcgcgcacgc gggcaaccac ttcggcgcgc
     3001 cggctgtcgg cgaccagctt ggtggtaacc ggtgcccgcc cgggcggcag ttcatcaatg
     3061 accgacacat cgaggtctgc atagtagctc atgcttaaag tccgcgggat cggcgtggcg
     3121 ctcatcatca actgatgagg ctgcaggccc ggtcggctgc ccttgatgcg cagcgccagg
     3181 cgctggtgca cgccaaagcg gtgttgctcg tcaataatgg ccaggcccaa tttacggaat
     3241 tccacctgct cctgaatcag cgcatgagtg ccaatgacca gcgtagcttc gctttgcaac
     3301 atgcttttca gcgcaacttg tttttgtttt ttactcaaac tgccggcaag ccacgccact
     3361 cttataccca ggggcgcaag ccagcctgaa aacttgtgat aatgctgctc ggcgagaatt
     3421 tcagtgggcg ccatgatcgc cgcctggcag ccgctctcga tgacctgcag caccgcaaaa
     3481 gcggcgacga tggttttgcc gctgccgaca tcgccctgca acagccgctg catcggataa
     3541 ggctgggaaa ggtcgcctgc gatttcctcc attgccctgc gctgggcagc ggttaaaacg
     3601 aacggtaatc cctgatgcaa ggcccgtgta agccgtccac gcttgggcaa ggccatggca
     3661 acgcggctgc ggcgctcgcg gtaatgcagg cgcatggata attgttgtgc cagcagttcg
     3721 tcaaatttga tcctgcgcca cgccggatga ccgcgctgca caagctgcgg caacttcacc
     3781 cgtggcggcg gctggtgcaa aaacaacacg ctttccctga aacccggcag cttgaacgcc
     3841 tgcaaaatct gccgtggcag gctctcggca agatcgtttt cggcgaggct gtgcttgatc
     3901 agctggcgca gggtgtcctg cgacagaccc gcagtcgtcg ggtaaaccgg agtcagcgaa
     3961 tccggcagcg gcatgtcgcc gcgcacgctg cggtatttgg gatgcaccat ctccgcgccg
     4021 aaaaaaccct ggcggatttc ccccaacacc cgcacgcgtg cgccgggcgc caatgctttc
     4081 acctggctgc cgtaaaaatt gaaaaagcgc atcaccagcg ggccggtgcc gtcctccacc
     4141 tggcagacca gcgtgcgccg cgggcggtat tggatttcgg tatttaccac tacgccttcg
     4201 acctgcacgg tctggccggt gggcgcgtct ctgatgggat aaagccgcgt ttcgtcctcg
     4261 tagcgcagcg gcaagtgcaa caccaaatcc agcggtgtag cgattcccag cttggcgagc
     4321 ttgtcctgcg tgctcttgga aataccggtg atggcgtgag gcgttcccgg tttctttgaa
     4381 gaataagcgg atccgccttt ctgtttttca cccctcaccc ttgactcctc acaccttact
     4441 cgctcagcac cagcaccgcg tccatttcca ccagcgcccc gcgcggcagc gcggccaccc
     4501 ccaccgccgc gcgcgccgga tagggttcgt gaaagtattt ggccataatt tcgttgactc
     4561 gcgcgaaatg gccgaggtca gtcaggaaca ccgtgagctt gaccacatta tccagaccgc
     4621 cacccgcagc ttgcgccact gctttcaggt tttcaaacac gcgcacaatc tgcgcttcaa
     4681 ttccctccac cagttgcatg ctcgccggat ccagcccgat ctggccggag agataaacgg
     4741 tttgacctgc gcgcactgcc tgcgaataag taccaatggc cttcggcgca gcattggttt
     4801 gaataatttt ttttgccata tcacgttcct tacaattatt cgccggctca acccgcgtgc
     4861 cgggcgtttt tagcgcaaaa aactcacggc atgttatcag gtttgcccgg tttcgacacc
     4921 gattttgcct cacggaatgc ccggattgcg ctatcataac cgcattcgca aatgttaact
     4981 caagtcccac ggtgagcccg ctcattgcaa atctggaaaa aatgctggcg gcgggccgcg
     5041 acgacgcatt gctgcgcttc agcctaggca acgaatatct gaaaaacagc gagtttgcgg
     5101 ctgcggcaca acacctgggc gaggcagtgc gtcatgatcc tttgtattcg gcagcgtgga
     5161 aacttttggg aaaagcattt gcggaatgcg gcaggctcga ggacgcactg aatgcgtatc
     5221 agcggggaat cgaggtggcc gaaaaaaaag gcgacaaaca ggccgcgaaa gagatgcagg
     5281 ttttcgcgcg ccgaattgaa aaacaattgg ctcaacagtc cgaatccggg caacaacaat
     5341 aacgcatgcc aatcatcgaa aaactccgtg agtttatagt cggtgccccg cgcgacccgc
     5401 ttaacccgga aacgcgccgt catatcgccc tgatcgcttt cttcgcctgg atcggcctgg
     5461 gcgcggatgg tttgtcctcc tcctgttacg gc
//
LOCUS       DATIOW010000094         3940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_858529, whole genome shotgun sequence.
ACCESSION   DATIOW010000094 DATIOW010000000
VERSION     DATIOW010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858529"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..489)
                     /locus_tag="VLL03_02935"
     CDS             complement(58..489)
                     /locus_tag="VLL03_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HSS46353.1"
                     /translation="MAYTLQKKRVPLLAYDETLTWVTLSLLGLGLVMVYSASIAIAEL
                     GKWHQPAYFLMRHAVFLGISIALAAIAFQLPMHLWQRISPYLFLLGAGLLVIVLIPGV
                     GREVNGSQRWLPLVFVNLQPSEFMKLLVVIYAADYTVRKAA"
     gene            complement(490..1896)
                     /gene="murD"
                     /locus_tag="VLL03_02940"
     CDS             complement(490..1896)
                     /gene="murD"
                     /locus_tag="VLL03_02940"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HSS46354.1"
                     /translation="MNVELSGKKVLVLGLGDSGLSMARWLTRKGAQVRVADSRKQPPH
                     AAQLAIEMPQVPLESGPFRDSSFSGIDMIAISPGVALRESRVSEALNAGVFVAGDVEL
                     FAQALKSFAEAKVIAVTGSNGKSTVTSMAGAMCRAADLNTVVAGNIGLPVLDALTQVE
                     TQMRAGKKAPDVFVLELSSFQLETTHSLDASAATVLNLCQDHLDRYSSMDEYAAAKAR
                     IFSGHGVQVVNRDDDFSRRLADPARTVLSFGLGKPGGNREWGLVASGDEPWLAQGEVK
                     LMPLEEMPLAGLHNASNALAALALCRAIDIKYAPLLSALKKITGLPHRVQKIAEIRGV
                     AFYDDSKGTNVGATVAALTGMPQKVVLIAGGDGKGQDFSPLKSAIAAHARALVLIGRD
                     ADKITKAVAGCGVPLLRAATMEDAVILSFSKAQRGDAVLLSPACASIDMFRNYEHRAE
                     VFVHAVQGLQAQPKVGAN"
     gene            complement(1902..2987)
                     /gene="mraY"
                     /locus_tag="VLL03_02945"
     CDS             complement(1902..2987)
                     /gene="mraY"
                     /locus_tag="VLL03_02945"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HSS46355.1"
                     /translation="MLLALAQWLAQDIRTFNVFNYITLRAVLATLTALMISFIVGPAM
                     IKKLTAYKIGQAVRDDGPISHLSKVGTPTMGGALILVSIALTTLLWADLRNRFVWVVL
                     VVTLGFGLIGWVDDYRKVVQHNPNGLSVRAKFFWQSVIGLAAALFLAFSTTLPAQTIF
                     IVPFFKHVAFPLGTVGFVVMTYLVIVGSSNAVNLTDGLDGLAIMPTVMVGSALGVFAY
                     VAGHAVFSKYLGFPFIPGAGELTVFCTALAGAGLAFLWFNVYPAEVFMGDVGALALGA
                     ALGVVAVIVRQEIVLFIMGGVFVVETLSVMLQVASFKLTGKRIFRMAPLHHHYELKGW
                     KENQVVVRFWIITMMLVLIGLSTLKLR"
     gene            complement(3032..>3940)
                     /gene="murF"
                     /locus_tag="VLL03_02950"
     CDS             complement(3032..>3940)
                     /gene="murF"
                     /locus_tag="VLL03_02950"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HSS46356.1"
                     /translation="LLEIRSQHRYAVIEMGMNRPGEIAYLTGLAKPDVALVNNVAPAH
                     LAGLGSLEQAAYAKGEIFEGLSKNGVAVINADDKYADLWRGLCAGKRVLDFGLNHPAV
                     VSARCDLRGSGAEIAVQTGASGMHINLQVTGLHNARNALAAATCALALGISREAISAG
                     LAVFTGVSGRLQRKPGKNGALLIDDTYNANPESVRAAVEVLSREAGKKILVLGDMGEL
                     GEQGPALHVQIGAQAKTAGVDVLYTLGELSAGAARQFGKGARHFTQIEELVAEVEKLM
                     APGVTVLVKGSRFMQMERVVERLAAR"
BASE COUNT          854 a         1265 c         1039 g          782 t
ORIGIN      
        1 tccgatcagc agcatcacca gcaacatggg caggaacccc ttgcggaagc tatgcatcta
       61 agcagccttg cgcactgtgt aatcagcggc gtaaatcacc accaacagtt tcatgaattc
      121 cgacggctgc aggttcacga agaccagcgg cagccagcgc tggctgccgt tgacttcgcg
      181 cccaacaccg ggtatcagca ctatcaccaa caggcccgcg ccaagcagga acaaataagg
      241 cgaaatgcgc tgccaaaggt gcatcggtaa ttgaaacgcg atcgctgcca gcgcgatgct
      301 tatcccaaga aataccgcgt gccgcatcag aaaataagcc ggctgatgcc acttgccgag
      361 ttcggcaatg gcaatggacg ccgaatacac catcaccagc cccagaccga gcagcgacaa
      421 tgtcacccag gtaagcgtct catcatatgc aagcagcggt acgcgctttt tttgcagcgt
      481 gtaggccatt tagttcgctc cgactttggg ttgcgcttgc aagccctgca ccgcgtgcac
      541 gaacacctcg gccctatgct cataattgcg gaacatgtcg atactggcgc aagcagggga
      601 gagcaacacc gcgtcgccgc gctgtgcttt gctgaaactc agtattaccg cgtcttccat
      661 ggtggctgcg cgcaacagcg gtacaccgca accggcaaca gctttggtga ttttgtctgc
      721 gtcccggcca atcagcacca gcgcgcgggc gtgagcggcg atcgcggatt tgagcgggga
      781 aaaatcctgt cctttgccat cgcccccggc gatgagcaca actttttgcg gcatgccggt
      841 cagcgcggcg acggtggcgc cgacattggt gcccttggag tcatcgtaaa acgccacacc
      901 gcgaatttcc gcgatctttt gcacacggtg cggcagccct gtaatttttt tcaacgcgga
      961 cagcagcggc gcgtacttta tgtcgatggc cctgcacaat gccagtgccg caagcgcgtt
     1021 ggaagcgttg tgcaaaccgg caagcggcat ttcctcaagc ggcatcagtt tcacttcccc
     1081 ttgcgcgagc cagggttcgt cacctgaagc gaccaagccc cattccctgt taccaccggg
     1141 ttttcccaaa ccgaaactca gaacagtccg cgcgggatcg gccaggcgcc ggctgaaatc
     1201 atcatcgcgg ttcaccacct gcacgccgtg gcctgagaaa atgcgcgcct tggcggctgc
     1261 gtattcatcc atgctgctgt aacggtcgag atggtcttgg cacagattca acaccgtggc
     1321 cgcgctggca tccaggctgt gggtggtttc cagttggaag ctggacagct ccagcacgaa
     1381 aacatcaggc gctttttttc ctgcgcgcat ttgcgtctcc acctgcgtca acgcatccag
     1441 caccggcagg ccgatgttgc ctgcaaccac ggtgttgagg tcggccgccc ggcacattgc
     1501 tccggccatg ctggtcaccg tgctcttgcc gttggagccg gtgacggcga ttacctttgc
     1561 ttctgcaaat gatttgagcg cctgcgcgaa aagctccaca tctccggcaa caaaaacccc
     1621 ggcgttaaga gcctcggaaa cccgagactc ccggagggca acgccggggc tgatagcaat
     1681 catatctatc ccgctgaaac tgctgtcgcg aaagggaccg ctctccagcg gcacttgcgg
     1741 catctcgatg gcgagttgcg cggcatgcgg cggctgcttg cggctgtcgg ccacgcgcac
     1801 ctgcgcgccc ttgcgggtaa gccagcgcgc catggacaaa ccgctgtcgc ctaatcccaa
     1861 caccagaacc tttttgcctg atagctccac gttcatttcc atcagcgcag tttcagcgtg
     1921 gaaagtccga tcaaaaccag catcatggtg atgatccaga accgcactac cacctgattc
     1981 tctttccagc cctttaattc atagtggtgg tgcagcggcg ccatgcggaa tatgcgtttc
     2041 ccggtcaact tgaatgaggc cacttgcagc atcactgaaa gcgtttccac cacaaacacg
     2101 ccgcccatga tgaacagcac gatttcctgg cgcacgatca ccgccaccac gccgagcgcg
     2161 gcgcccagtg ccagcgcgcc gacatcgccc atgaacactt ccgccgggta gacgttgaac
     2221 cacaggaaag ctaaacccgc ccctgccagc gccgtgcaaa ataccgtcaa ttcccccgca
     2281 ccgggaatga aaggaaaccc gagatattta gaaaagactg cgtgaccggc aacataagcg
     2341 aacacaccga gcgcgctgcc caccatcacc gtgggcatga tcgccaggcc gtccaggcca
     2401 tcggtgaggt tcaccgcatt gctcgatccc acgatcacca gataagtcat gacgacaaaa
     2461 ccgaccgtac ccagcggaaa agctacatgc ttgaagaacg gcacgatgaa tatggtttgc
     2521 gccggcagcg tggtgctgaa cgcgagaaac agcgcggcag caagcccgat caccgactgc
     2581 cagaaaaatt tggctctcac cgataagcca ttggggttgt gctgcacgac ttttcgataa
     2641 tcgtccaccc agccgataag gccgaagccc agggtgacca ccagcaccac ccacacaaag
     2701 cggttgcgca aatccgccca cagcaaagtg gtcaaagcga tagaaaccag gatcaatgca
     2761 ccgcccatgg tcggcgttcc gactttgctc aagtgcgaaa tcgggccgtc gtctcgcacc
     2821 gcctgcccga ttttgtaggc ggtgagtttt ttgatcatcg ccgggccgac gatgaaagaa
     2881 atcatcagcg cggtgagtgt cgccagcact gcgcgcagcg tgatgtaatt gaacacgttg
     2941 aaggtgcgga tgtcctgcgc cagccattgt gcgagtgcaa gcaacatggg ctatttccat
     3001 tcccgggcgt tgagcccgcg taaaacgctc gtcatcgggc agccaacctt tccaccactc
     3061 tttccatctg catgaagcgc gatcccttca ccagcaccgt gacacccggc gccattagtt
     3121 tttcaacctc ggccactaac tcctcgattt gcgtaaaatg cctcgctcct ttgccgaatt
     3181 gccgcgccgc gccggccgaa agttctccca gggtgtacag cacatccacg ccggcagttt
     3241 ttgcctgcgc gccgatttgc acgtgcaacg cagggccctg ctcaccaagc tcccccatgt
     3301 cgcccagcac cagaattttt tttcccgctt cgcggcttaa aacttccacc gccgcgcgca
     3361 ccgactccgg gttggcgttg taggtgtcat caattaatag cgcgccattt ttgcccggct
     3421 tgcgctgcag cctcccgctc accccggtaa atacggcaag tccggcacta atcgcttctc
     3481 ggctgatacc cagcgccagc gcgcaagtcg ctgcggcaag tgcgttcctc gcgttatgca
     3541 gtccggtgac ctgcaagttg atatgcattc cacttgcgcc ggtttgcacc gcgatttcgg
     3601 ccccactgcc gcgcaaatcg cagcgtgcgc tcaccaccgc gggatggttt aagccgaaat
     3661 cgagcacgcg cttacctgca cacagcccgc gccacaaatc cgcgtattta tcgtcggcgt
     3721 tgattaccgc cactccgttt ttggaaagtc cttcaaaaat ttctcctttg gcgtacgccg
     3781 cctgttccag cgaacctagg ccggcgagat gggccggcgc gacgttattc accagcgcca
     3841 catcaggctt ggccaggccg gttaaataag cgatttctcc cggacggttc atgcccattt
     3901 caatcaccgc gtaacgatgc tggctgcgta tttccagcaa
//
LOCUS       DATIOW010000095         3286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1569956, whole genome shotgun sequence.
ACCESSION   DATIOW010000095 DATIOW010000000
VERSION     DATIOW010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569956"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VLL03_02955"
     CDS             complement(<1..729)
                     /locus_tag="VLL03_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HSS46357.1"
                     /translation="MQITSMYFKARAAEKLHDKQLQQALLKAQGKFVHARAAAVRELD
                     NFEVYRDTAAAIRDRVLLDLDVYLEQFEQTAAARGAEVHWAETTEEANCIVCEIATRH
                     GVKKAVKSKSMVSEECALNEALEAAGIEVVETDLGEYILQLAHEPPSHIVAPVVHKTK
                     DEISDLFEQKHNKPRKTGIAELCREAREVLRPHFLSADMGISGANFLIAETGSTLIVT
                     NEGNGRLATTMPRVHVAVTGIEKIV"
     gene            complement(788..1519)
                     /locus_tag="VLL03_02960"
     CDS             complement(788..1519)
                     /locus_tag="VLL03_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HSS46358.1"
                     /translation="MRVGLFVTCLVDLMRPRIGFAALRLLEAAGCEVIVPSSQTCCGQ
                     PGYNSGDRASAKALALKFVREFAACDYVVAPSGSCAGMVRVHYSDLFEGEPELLEQAQ
                     KLARRTFELTDFLVNVAKLEKLPSAFSGSVTYHDCCSGLRELGVKQQPRALLSRMPGV
                     ALKEMQNCETCCGFGGTFAVKFGEISTRLADNKCHDIEISGAEAVVLGDLGCILNIEG
                     RLRRRGNRKTRVLHVAEVLAGIGEQ"
     gene            complement(1647..2627)
                     /gene="egtD"
                     /locus_tag="VLL03_02965"
     CDS             complement(1647..2627)
                     /gene="egtD"
                     /locus_tag="VLL03_02965"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HSS46359.1"
                     /translation="MRGPVAGETVRFYSLLQKQKSFLDEILEGFSRARKQIPAKFFYD
                     ALGCGLFDAICELPEYYLTRVETAMMREYAAEMAQCLGPDCLLIEYGSGASRKTRLLL
                     DHLRPPAYMPIDIAGEQLRASAQALAGAYPGMQVLAVCADYSQAIKLPVRDATGIKRK
                     AIYFPGSTIGNFTVEETVAFLQQARGVTGRGGAMLVGVDLKKSKEVLHAAYNDAQGVT
                     AKFNLNLLTRINRELGADFNLSNFRHHAFYNEQAGRIEMHLESLVAQQVKIGDRRFDF
                     RAGETIHTENSYKYSVAEFQDLARKAGFAPQRSWTDAQQLFAVHYLHAGG"
     gene            complement(2634..>3286)
                     /locus_tag="VLL03_02970"
     CDS             complement(2634..>3286)
                     /locus_tag="VLL03_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HSS46360.1"
                     /translation="AMVVLDAQLPLDEPVTIGKEDVDTKRGTRSKLKVGVMLSRGELL
                     QLALMASENRAAAALAHSYPGGVPAFVAAMNQKAAALDMAQTHFVDPTGLDRRNVSTA
                     QDLVKMVKASYGYDLIREYTTATSQAVAGAQRKSRALSFHNSNRLVKSKSWEIGLSKT
                     GYIHDSGRCLVLQTRIADQPLIIVLLDSWGKYTRIADANRIKRWIETSLARKPRSS"
BASE COUNT          660 a          967 c          933 g          726 t
ORIGIN      
        1 cacgattttc tcgatgccgg taaccgccac atgcacgcgc ggcatggtag tcgccaagcg
       61 gccgttgcct tcgttggtaa caatcaaggt ggaaccggtt tcggcaatca ggaagttggc
      121 accggaaatt cccatatcag cggaaagaaa atgcggtcgc aagacctctc gtgcctcgcg
      181 gcacagttcg gcgatgccgg tcttgcgcgg cttgttgtgc ttttgctcaa agagatcgga
      241 gatctcgtcc ttggtcttgt gcactaccgg cgccacgatg tgcgaaggcg gctcgtgcgc
      301 cagttgcagg atgtattcac cgagatcggt ttccactact tcgatccccg ccgcttccag
      361 cgcctcgttg agagcgcact cttcgcttac catggacttt gatttgaccg cttttttcac
      421 cccgtggcgc gtggcgattt cgcacacgat gcagttcgct tcttcggtgg tctcggccca
      481 gtgtacttcc gcgccgcgcg cggcggccgt ttgctcaaat tgctcgagat aaacatcgag
      541 atcaaggagc acacggtcgc gtattgccgc tgccgtgtcg cggtacactt cgaaattatc
      601 cagctcgcgt accgctgccg cgcgcgcatg aacgaatttg ccctgcgctt tcagcagtgc
      661 ctgctgcaac tgcttgtcgt gcagcttttc tgcggcgcgc gccttgaagt acatggaagt
      721 gatttgcact aaattttctc cgaaaatttt tgtgaatggt aaacggtaaa tggtgattag
      781 gttttattca ctgttcacct atccccgcca gtacttcggc cacgtgcagg acacgggttt
      841 ttcgattgcc gcggcgacgc aaccggcctt cgatgttcag aatgcagccc aaatcgccga
      901 gcaccaccgc ttccgcgccg ctgatttcga tgtcatggca cttgttgtcg gccagccgcg
      961 tggaaatctc accgaacttg accgcaaaag tgccgccaaa gccgcagcaa gtttcgcagt
     1021 tttgcatttc cttgagtgcg acacccggca ttctcgacaa caacgctcgc ggctgttgtt
     1081 tcacgcccag ctcgcgcaga ccagagcagc aatcgtgata agtcacgctg ccgctaaaag
     1141 cactgggcag tttttccagc tttgccacat tcaccaggaa atcggtcaat tcaaaggtac
     1201 gccgcgcgag tttttgcgcc tgctcgagca gctcaggttc gccttcgaac agatcggaat
     1261 aatgcacgcg caccattcct gcgcaggaac cggagggcgc gactacataa tcgcaagcgg
     1321 cgaattcccg gacgaatttc agcgccagcg ctttggcgga agcacggtcg ccggaattgt
     1381 agcccggctg gccacagcag gtttggcttg aagggactat cacttcgcag cccgcggctt
     1441 ccagcagcct aagcgcggca aaaccgatgc gcgggcgcat cagatcaaca aggcaggtca
     1501 cgaaaagccc gacacgcatc gcttttccct aattaagaat tttcagaaaa gtatgcgcct
     1561 aatcatagca cggagctctt gcttgctact gacaaaagtc gcgcgaaaat catcaggcgc
     1621 gtgatgggca catcggcgcc cgggtatcag ccgcccgcat gcagataatg cacggcaaaa
     1681 agctgctgcg catcggtcca cgaccgttgc ggcgcgaatc ccgcctttct tgccagatcc
     1741 tgaaattctg ccaccgagta cttgtaggaa ttttcggtgt gaatggtttc acccgcgcga
     1801 aagtcgaagc gccggtcccc tatctttact tgctgggcaa cgagactctc gagatgcatt
     1861 tcaatgcgcc ctgcctgttc gttgtaaaac gcgtgatgcc ggaaattgct caagttgaaa
     1921 tcggcgccga gctcgcggtt gatgcgggta agcaaattga ggttgaattt tgccgtcacg
     1981 ccttgtgcat cgttgtaggc ggcgtgcaaa acttctttcg attttttcaa atccacgccc
     2041 accagcatcg cgccgccccg gcccgtcact ccccgtgcct gttgcagaaa cgccacggtt
     2101 tcctccactg tgaaattgcc gatggtggag ccgggaaaat aaatcgcttt gcgcttgatc
     2161 cccgtcgcat cgcgcacggg caacttgatg gcctgtgaat aatcggcaca cacggccagc
     2221 acttgcatgc ccggataagc acccgcgagc gcctgtgccg aagcgcgcaa ctgctcgccg
     2281 gcaatatcga tcggcatgta ggcgggaggg cgcaggtggt cgagcagcag gcgcgttttg
     2341 cggctggcgc cgctgccgta ttcgatgagc aggcaatcag ggcccaggca ttgcgccatt
     2401 tccgcggcat actcgcgcat catagcggtc tcgacgcgcg tcaaataata ttcgggaagc
     2461 tcgcagatgg catcgaacag cccgcagccg agcgcatcat aaaaaaactt ggcgggaatc
     2521 tgctttcgcg ccctggaaaa cccttcgaga atttcgtcga gaaagctctt ttgcttctgg
     2581 aggagggagt aaaaccggac ggtctcaccc gcaaccgggc ccctcattgc cgcctaactc
     2641 gagcggggtt tgcgggccag actggtttct atccagcgct tgattcggtt ggcgtcggca
     2701 atccgggtgt acttgcccca cgaatcgagc agcacgatga tgagcggttg gtcggcgatg
     2761 cgtgtctgca gcaccaggca gcgtccggaa tcgtggatat agccggtttt cgacagcccg
     2821 atctcccagc ttttgctttt gaccaatcgg ttggagttgt gaaaggaaag ggcgcgcgat
     2881 ttgcgctgcg cgccggcgac ggcctgcgag gttgcggtgg tgtattcgcg gatcaggtcg
     2941 tagccgtagc tggccttgac catcttgacc agatcctgcg cggtggaaac attgcggcgg
     3001 tcgaggccgg tgggatcgac aaaatgcgtt tgcgccatgt caagcgccgc ggccttctgg
     3061 ttcatggctg ccacaaacgc cggcactccg ccggggtaac tgtgcgccag cgcggctgca
     3121 gcgcggtttt ccgaagccat cagcgcgagc tgcagcaatt ccccgcgcga caacataacg
     3181 ccgaccttga gcttggaacg ggttccgcgc ttggtgtcca cgtcttcttt gcctatggtg
     3241 accggttcat ccagcggcaa ttgtgcatcg agcactacca tcgccg
//
LOCUS       DATIOW010000096        17797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_249259, whole genome shotgun sequence.
ACCESSION   DATIOW010000096 DATIOW010000000
VERSION     DATIOW010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17797
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249259"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1757)
                     /locus_tag="VLL03_02975"
     CDS             complement(51..1757)
                     /locus_tag="VLL03_02975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS46361.1"
                     /translation="MAVAGKATLSEAFLASRQQWFLGLMLLALHVVLVLGIESWLARG
                     FVLTHFGLFLIWQPIWGGERNLDLRHGVLIIIAGLAFAVWNSWWLMAVWIAVLFALIG
                     GNVPGKRQRGQRALSLLAAVYLLAVLLMWVVPHLFDDIRFPEAMTLVVRYGLLLPVFA
                     IFFIKAENEPESSQRAVDLSYSLLLFLLTAALVLGSFVIKQVSQGGYVLSLAQTLFLI
                     AMMILALSWLWDPHSGFDGVGQLFSRYLLSVGLPFERWMHSLANLADREGNPEKFLIM
                     SAHEITALPWVAGVSWRVAGSERKLGGTSQFPAEFSFGDLHMVLYSKWQLSPALLLHV
                     KLLVRLLADFYSAKIRERQERQNAYTQAVYETGARLTHDVKNLLQSLKSLCTAVETGG
                     ADDAEALRALIHRQLPQITQRLQLTLDKLKAPQKADEGNGKSAQEWWANLKQRYSGEA
                     IDFKETDISASAELPTQLFDSVADNLLQNALQKSKLEPGLRISVGFSCATGATLSVCD
                     NGVPLSESLVKTLFNGPVPSQNGLGVGLYQAARQAVQMGYHLRLASNQIGKVCFELAP
                     AK"
     gene            1867..2646
                     /locus_tag="VLL03_02980"
     CDS             1867..2646
                     /locus_tag="VLL03_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSS46362.1"
                     /translation="MNSARRQAITIARYTLLEALRTRLPWLMLATLLLALLGSLFVKE
                     IAITETLRQQISFLAAALRLAAVFILALYIVSSMVREFNDKNLELLLSLNLPRAAYAC
                     GKFFGFSLVALITVALICLPLAWLAPLQPVLLWGLSLTLELWIVAAMGFFCIITFTHI
                     MPAASFVMAFYVLARSITAIRLISGSTLFDQQSLSHQVTVFLVDSLSLVLPNFDAFTQ
                     TAWLVNQSGEWHMLLPIVVQSTIYTGLLLGAALFDLYRKNL"
     gene            2649..3374
                     /locus_tag="VLL03_02985"
     CDS             2649..3374
                     /locus_tag="VLL03_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46363.1"
                     /translation="MPERSISAVPKPVWWLLLASLCLQLAWQGLQPRPVARAEALVFP
                     PETPALKALSLGEPIALSQLMTLYLQAFDNQPGISIPFKDLDYERVEAWLSRILELDP
                     VGQYPLLMASHLYAQVPDVQKQRRMLQFVYAQFLHDPNRRWPWLAHAAIMAKHRLHDL
                     PLALKYAQAISTHAFGPEVPHWATQMRIFLLEDMGEVESVKVLLGGLLVSGVITDPHE
                     IHFLTERLQQLEAAEKSSKTPEK"
     gene            3430..4272
                     /locus_tag="VLL03_02990"
     CDS             3430..4272
                     /locus_tag="VLL03_02990"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSS46364.1"
                     /translation="MTTILYIAKLRPNVGPGPWSKAENLGKTRGWAWYLLAEYDTKTT
                     LSGEGMAKMVRKHSGFTLIEIAIVLVIIGLLLGGVLKGQELITSARVRNLISEQDGIK
                     AAFFGFQDRFRSFPGDYTLASTNINCTPACSNGNGNGRIEATGATIAENIAVWEHLSR
                     SGFINGTYTYAATASDSTSPKNPYAVYVNVVFDAAYGDPAVVTTPQRHNIKTGGQVPV
                     EIIAEVDRKVDDGLPYSGAFQFSNYQGNAAAAPTVSCVNTATTPTSWAVTSGVTNCGG
                     ASLL"
     gene            4479..5180
                     /locus_tag="VLL03_02995"
     CDS             4479..5180
                     /locus_tag="VLL03_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSS46365.1"
                     /translation="MWHRQSGFTLIEIAIVLVIIGLLLGGVLKGQELITSARVRNLIS
                     QQNGIKAAFFGFQDRFRAFPGDYAQATVNISGVSTSVCGGGNGNGNGRIEFTNSENIL
                     AWEHLSKAGFINGTYTCAATESSTTSPVSPYGIYLQLIYDGVYGITSGGVPQHNLKTG
                     GQIPVEIISEVDRKIDDGVPYTGAFQFSAYQGNAAAAPTEGSTTAPACTSSNTTAAVW
                     NSTNGSSNCGGTSIF"
     gene            complement(5236..7200)
                     /locus_tag="VLL03_03000"
     CDS             complement(5236..7200)
                     /locus_tag="VLL03_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HSS46366.1"
                     /translation="MQTTLQLVLILLATAVLVVVLFRVLKLPPLLGYLLVGILIGPHA
                     LAWIHEDAGTRHLAEFGVVFLMFSIGLEFSLPKLITMRRIVLGLGAAQVVLTLLIVLL
                     AGFAMGLEWRAGLALGGALAMSSTAIVSKMLSERLELTTEHGRQVIGVLLFQDLAVVP
                     LLIIVPALAEGSDIAAALGIAALKAVVVLALLLFLGQRLMRAWFHLVARQKSSELFVL
                     NVLLITLGLAYLTQLAGLSLALGAFLAGMLISETEYRYQVEDDIKPFRDVLLGLFFVT
                     VGMLLDVRSVLENWLAVGGLLVALVLSKIMLIAGLSRGFGSQPGTALRTALCLAQAGE
                     FGFVLLWQAGGLRLLDAEILQVVLAAMLLSMLAAPFIIERAEHLALHFSGAEWMNRAM
                     QLHHIAVQTLAAERHVIICGYGRSGQNLARILERENILFVALDLDPQRVREAAAAGDN
                     VVYGDAGRREVLIAAGLMRAKALVVAESNVPLALKILSHVQEARPDLPVLVRTLDDAD
                     LEQLKEAGAAEVVAEIMEGSLMLASHALILLGVPLNRVLKRIRETRERRYRLFHGLFR
                     GMADEPEGGEKQQQRLRSVVIAAGAAALGKTLGEINLAKLAVEVTAVRRRNIRGLDPS
                     PETRLEAGDVLVLLGTEDNLAAAEIKLLQG"
     gene            7678..8676
                     /locus_tag="VLL03_03005"
     CDS             7678..8676
                     /locus_tag="VLL03_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HSS46367.1"
                     /translation="MSSVKKPAKSALPQTLALARQVLEIEARGIVALIDRLDQQFLRA
                     VELILHCRGRVVVSGIGKSGHIARKIASTLASTGTPAFFVHPAEASHGDLGMVMREDV
                     LIALSNSGESTELLTIVPILKRQGAKLISITGNPRSSLAVEADVHLDATVAEEACPLG
                     LAPTASTTAALALGDALAVALLDARGFGIEDFARSHPGGALGRKLLTHVRDVMRMGDA
                     MPVVSENATFPQAILEMSKKGMGMTAVLNTRQSVVGIFTDGDLRRALEKITDLRSVMV
                     ARVMTRNPRTIAPDRLAVEAVEMMESHKINQLLVVDADQRLIGALNMHDLFRAKVI"
     gene            8757..9281
                     /gene="kdsC"
                     /locus_tag="VLL03_03010"
     CDS             8757..9281
                     /gene="kdsC"
                     /locus_tag="VLL03_03010"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HSS46368.1"
                     /translation="MQAVFEKAKRVRLALFDVDGVLTDGSLYISDSGEEYKAFNTMDG
                     HGMRMLKASGVELAIITGRTSRCVEMRAQNLGIGLLYQGVDNKLEVYDSLLKKFALSS
                     DATAFVGDDVVDLPVMRRAGLAICVPESPALVKQHAHYVTQAAGGRGAVREICELIMQ
                     AQETYSAQIAPYLK"
     gene            9278..9850
                     /gene="lptC"
                     /locus_tag="VLL03_03015"
     CDS             9278..9850
                     /gene="lptC"
                     /locus_tag="VLL03_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HSS46369.1"
                     /translation="MNNRLLIWAPLGMLFLLAALTWWLDSKVQPPQPKRDGSSRHDPD
                     YIVEDFTTTRMSKDGMPRYILRAKKMVHYPDDDSTHLDYPDFRHVETGKPELRITSDQ
                     GFLSSGGEDAYFIGDVKVVREPDAEYGELRLSTSFLHVIPDQDLAKTDKPVTLTDPHT
                     IINAVGLELNSNTRELKLLSQVRGHYENPK"
     gene            9834..10436
                     /gene="lptA"
                     /locus_tag="VLL03_03020"
     CDS             9834..10436
                     /gene="lptA"
                     /locus_tag="VLL03_03020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HSS46370.1"
                     /translation="MRIQSKPRSLPLWALLGVLFCAPAAAERADRDKPVNVEADKVNV
                     DDANKVSTFEGNVVLTQGTIRIRADKMIVRQNEDGFNSGTAYGNLATFRQKMEGVDKF
                     IEGEAERIEYDGKAEKLELFTRAHVKRGQDELRGNYISYNAQTEFYQALSGKPATASE
                     TKPGRVRAVIQPKDKNNKPDTPASPATLKPSSGIVNPPQE"
     gene            10485..11207
                     /gene="lptB"
                     /locus_tag="VLL03_03025"
     CDS             10485..11207
                     /gene="lptB"
                     /locus_tag="VLL03_03025"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HSS46371.1"
                     /translation="MSELKAQKLRKRYKSRTVVRDVSLEVRSGEVIGLLGPNGAGKTT
                     CFYMMVGLVPLDGGSITLDERNLSHMPIHRRARLGLSYLPQEASIFRRMTVAENIQAV
                     LELQDFQPDEIENRLDDLLHDLGIAHLRNNPAMSLSGGERRRVEIARALASNPRFILL
                     DEPFAGVDPIAVLDIQKIIGFLKEKGIGVLITDHNVRETLGICDRAYIINDGSVLAYG
                     KPDEIVYNESVRKVYLGEHFRL"
     gene            11265..12713
                     /locus_tag="VLL03_03030"
     CDS             11265..12713
                     /locus_tag="VLL03_03030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HSS46372.1"
                     /translation="MKHTLQLKLSQHLTLTPQLQQSIRLLQLSTLELNQELERFLQEN
                     PLLERDDEVADETPTVYPTGNDALADSASPANERPVESTQETTLSAADMVEPVWFEEA
                     DFSSAREDSDEAEYPQLAAVSPTLREHLHWQLALTKLVERDNKLVSLLIDALDEDGCL
                     TQPLEELVALLREELSVTLDELQIALKHLQKFEPAGVGARNLGECLKLQLEALPEDTL
                     YRAEALKIVTEHLDSLASRDFAKLKKNLHCDDHTLRQAQHLITSLNPRPGADYAQVET
                     RYVVADVVVKKVKGIWVANLNPDAMPKLRLNRMYADILHRNRDSSFQQLAPQLQEAKW
                     LIKNVQQRFDTILRVSQAIVDRQRHFFEHGEVAMRPLVLREIAEALSLHESTVSRVTT
                     QKYMLTPRGIYELKYFFGSHVATETGGACSATAIRALIKQLVAAEEPKRPLSDSQISE
                     ILGQQGIVVARRTIAKYREALQIPPVSQRKSL"
     gene            12730..13053
                     /gene="raiA"
                     /locus_tag="VLL03_03035"
     CDS             12730..13053
                     /gene="raiA"
                     /locus_tag="VLL03_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HSS46373.1"
                     /translation="MNLNLTGHHLEITPSIRDYIVSKLDRITRHFDHVIDVNVILSVE
                     KLKQKIEVSVHVRGKDIFAECDNADMYAAIDLLVDKLDRQILKHKEKHSEHRHNGALK
                     HQSHD"
     gene            13072..13623
                     /locus_tag="VLL03_03040"
     CDS             13072..13623
                     /locus_tag="VLL03_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HSS46374.1"
                     /translation="MQLIPSRRGGASGDTADAEKASLVARLLPPANVVLDLDVASKKR
                     VFEQAGLLFENNHGIARSQVFDALFAREKLGSTGLGHGIAIPHGRIKSLKEALGAFFR
                     TKAPVPFDAPDGLPVNLVFVLLVPERATDLHLQILSELAQMFSDRNLRETLAACSDPV
                     LLHQLLSGWQPHAPGQRSPAVSR"
     gene            13586..14533
                     /gene="hprK"
                     /locus_tag="VLL03_03045"
     CDS             13586..14533
                     /gene="hprK"
                     /locus_tag="VLL03_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HSS46375.1"
                     /translation="MPQVSAALLFQDNQERLGLTWIAGEENRLLTSEDIANSSNGVVG
                     HLNFIHPNWVQVLDRAEIEYLNRLDAKTMQQSLQGLAVSNPFCFILAREARAPAALKK
                     LTDEKRIPLIASTQGSLYLMNVLRVYLSKVLAEMTNLHGVFLDVLGMGVLLTGESGVG
                     KSELALELISRGHGLVADDSVELYLIAPDTLEGRCPPLLRDFLEVRGLGVLNIRTIFG
                     ETAVRRKMNLKLIANLEKPSGTHIPGLERLPLGASSEDILSVKIRKVTIPVAEGRNLA
                     VLVEAAVRNYILQLRGIDSTQEFIKRHEQMMADPPGGEE"
     gene            14549..15385
                     /gene="rapZ"
                     /locus_tag="VLL03_03050"
     CDS             14549..15385
                     /gene="rapZ"
                     /locus_tag="VLL03_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HSS46376.1"
                     /translation="MQLILITGLSGSGKSIALNVLEDSGYYCVDNLPAKLLPEITRFL
                     REAGHDRVAVSIDVRSGDTFNQLPQQINALRSQGVDVRPLFLEAKNETLVKRFAETRR
                     RHPLQDDTRTLAECIALERERLADIGDLAQHIDTSDLNPNALRGWIKDLVKIDRSRLT
                     LLFQSFGFKNGIPLDADLVFDVRCLPNPHYDPVLRELTGKDPAVVEFLHKDKNVLDML
                     ADIQRFIENWLPCFIRDNRYHLTVALGCTGGRHRSVYFAEKLAAHFRSKNQVLVRHRE
                     LA"
     gene            15390..15764
                     /locus_tag="VLL03_03055"
     CDS             15390..15764
                     /locus_tag="VLL03_03055"
                     /inference="COORDINATES: protein motif:HMM:NF018685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HSS46377.1"
                     /translation="MNLFRYTKPGDWLVFSCGLLLCLGLILTLWRHDAPQRAIIRSGG
                     KIFAEVRLPANQSFAISGPLGTTFVSIRNLEARISSDPSPRQYCVKQGWLKSAGETAV
                     CLPNQVSLELLGRNTSYDSLNY"
     gene            15745..16296
                     /locus_tag="VLL03_03060"
     CDS             15745..16296
                     /locus_tag="VLL03_03060"
                     /inference="COORDINATES: protein motif:HMM:NF019099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HSS46378.1"
                     /translation="MTRSTIELMAFPEDHRIAKMAAYALALTLLETGIPSPLPGVKPG
                     LANIVTLVVLARYGLRFAVWVSLLRVVCASLLIGTFLSPSFFLSLSGAIASLVMLACT
                     RVLPCRLFGPVSHSILAALAHIAGQLAVVYLWLIPHPGIFYLAPVFAAAALMFGTVNG
                     LVAAKWLESANDEPRLAGAAQQI"
     gene            16337..16975
                     /locus_tag="VLL03_03065"
     CDS             16337..16975
                     /locus_tag="VLL03_03065"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HSS46379.1"
                     /translation="MNDPTAAPFSMISMKTVTLAFTGASGMPYGVRLLESLLAGKMRV
                     YLLYSPAAQIVAQQEMGLALPSRAKEAQSFFREKYGVDENALKVFGREEWFAPVASGS
                     NPADAMIICPCSMGTLAAGLSDNLIERAADVTLKEARKLILVPRETPLSAIHLENMLK
                     LSRAGAVILPANPGFYHHPQNIQDLVDFVVARILDHLGIPHQLAARWGKEAN"
     gene            16996..17616
                     /locus_tag="VLL03_03070"
     CDS             16996..17616
                     /locus_tag="VLL03_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HSS46380.1"
                     /translation="MNLGLFSKSAPALETLPIFPLNTVLFPGGILMLKIFEPRYMDMS
                     KVCLRDNKPFGVCLIKEGNEVGAPAMPEETGCTAGITDWDMQQLGVLHLKTEGRQRFR
                     ILGRQVAKDGLISAEVAMLGAEPETPLASDLRQCATVLKLIMEKIGEEHFPAPAKLDD
                     ATWVGYRLAEVLPLKLAAKQKMLEINDSIVRLQVLHKFLVQQGLVA"
BASE COUNT         3847 a         5498 c         4957 g         3495 t
ORIGIN      
        1 tcctattcaa ccgcatggtg gctgccggca agctgcccta agtctatcgc ttatttcgcc
       61 ggtgctaatt caaagcacac tttgccgatt tgattgctcg ccaagcgtag gtggtacccc
      121 atttgtacag cctggcgcgc cgcctggtaa agacccactc ccagcccgtt ctgcgaaggc
      181 accgggccgt tgaaaagcgt tttgacgagg gattcggaaa gcggcacccc gttatcgcaa
      241 acgctcaagg tggcccccgt ggcgcaggaa aaaccgacgc tgattcgcaa tcccggttca
      301 agcttgctct tttgcagggc gttttgcaga agattgtcgg cgacgctgtc gaacagctgc
      361 gtcgggagct cggcgctggc ggaaatatcc gtttccttga aatcaatggc ttcgccggag
      421 taacgctgct tgaggttggc ccaccactcc tgagccgact tgccgttgcc ttcgtccgcc
      481 ttctgcggcg ctttgagttt atccagagtc aactgcaggc gctgggtgat ttgcggcaac
      541 tggcggtgga tcagcgcacg cagcgcctcg gcatcatcgg caccgccggt ttccacggca
      601 gtacataatg atttcagcga ttgcaaaagg tttttcacgt catgggtaag gcgcgcgcca
      661 gtttcataca ccgcctgcgt gtaagcattc tgccgctcct gcctttcgcg aatcttggca
      721 ctgtaaaaat ccgccagcag ccgcaccagg agcttgacgt gcagcagcag cgccggactc
      781 agctgccact tgctatacaa caccatgtgc aggtcgccaa aggaaaattc ggccgggaat
      841 tgggaagtgc cgccgagctt gcgctcgctt ccggccactc gccagctcac tcccgccacc
      901 catggcagcg cggtgatttc atgtgccgac atgatcagga atttttccgg attgccctca
      961 cggtcggcga gattcgccag gctgtgcatc cagcgctcga acggcaatcc gaccgaaagc
     1021 aggtagcgcg agaagagctg gcccaccccg tcaaaaccgg aatgcgggtc ccacagccag
     1081 cttaatgcta atatcatcat cgcaattagg aatagcgttt gagccagcga tagaacatac
     1141 ccgccttggc tcacctgctt gatgacgaat gaccccagca ccagcgcggc agtaagcaaa
     1201 aacaagagca agctgtagga taaatccacc gcccgctggc tcgattcagg ttcgttctcc
     1261 gccttgatga aaaaaatcgc aaataccggc agcagcagcc cgtagcgcac caccagcgtc
     1321 atagcctcgg gaaacctgat gtcgtcgaac aaatgcggca ccacccacat tagcaacacg
     1381 gccagcaaat agacggccgc gagcagggaa agcgcgcgtt gcccacgttg ccgcttgccc
     1441 ggcacattgc cgccgatcaa cgcgaacagc acggcaatcc ataccgccat cagccaccaa
     1501 ctgttccaga cggcgaacgc cagcccggct atgataatga gcacgccgtg gcgcagatcc
     1561 agattgcgtt cgccgcccca aatcggctgc catatcaaaa acagcccgaa atgcgtaagc
     1621 acaaaaccgc gcgccagcca gctttcgatg ccgagcacca gcaccacatg cagcgccagc
     1681 agcatgaggc cgaggaacca ttgctggcgc gaggccagga atgcttcgct cagggtcgct
     1741 tttccggcaa ccgccatatc ccgttacccg gcacaaaaat tgttaaagta gcagcccgca
     1801 ttttcagatt tgccccgctc aatttgagtt tatacttcac tccttgaatt tatgccaacg
     1861 cccatcatga attccgcccg ccgccaggca ataaccattg cccgctatac gctgctcgag
     1921 gcgctgcgca cccgcttgcc gtggctgatg ctggcaacgc tgttgcttgc gctgctgggc
     1981 agcctgttcg taaaagaaat cgccatcacc gaaacgctgc gccagcaaat ttcgtttctc
     2041 gccgctgcac tcaggctggc cgccgtgttc atcctcgcac tttacatcgt gagcagcatg
     2101 gtacgcgaat ttaacgacaa aaaccttgag cttttgctct ccctcaattt gccgcgcgcc
     2161 gcctacgcct gcggcaagtt tttcggcttt tccctggtcg ccttgataac ggtcgcgctt
     2221 atctgcctgc cgctggcctg gctggcaccg ctgcagcctg tgttgttgtg gggcttgtcg
     2281 ctcacactcg agctttggat tgtggccgcg atgggctttt tttgcatcat caccttcacc
     2341 cacatcatgc ccgccgccag ctttgtcatg gccttttacg tgctggcgcg ttccatcacc
     2401 gcaattcgct tgatcagcgg ctccacgcta ttcgaccagc aaagcctgtc gcaccaggtt
     2461 acagtattcc tagtggacag tctgtcactg gtgcttccca acttcgacgc attcacccag
     2521 actgcgtggc tggtgaacca aagcggtgaa tggcacatgc tgctccccat cgtcgtacaa
     2581 agcacgattt ataccggctt gctgctgggc gcggcattgt tcgacctcta ccgaaaaaac
     2641 ctgtagcaat gccagaacgt tctatttccg cggtgccaaa accggtttgg tggctgctac
     2701 tggcaagcct gtgcttgcag ttagcttggc aaggtcttca gccgcggccg gtggcccgcg
     2761 ccgaagcgct ggttttccct cctgaaacac cggcgctcaa agcgttgagc ctgggtgagc
     2821 cgattgcctt gtcacagctt atgacgctat accttcaggc gttcgataac cagccgggca
     2881 tcagcatccc gttcaaggac ctcgactacg aacgcgttga agcctggctt tcgcgcattc
     2941 tcgagcttga ccccgtcggc cagtatccgc ttctcatggc ctcccatctc tacgcccagg
     3001 taccggatgt gcaaaagcag cgaagaatgc tgcaattcgt ttatgcgcaa tttttgcacg
     3061 accccaaccg gcgctggccg tggctggccc acgccgcaat catggccaag caccggctgc
     3121 acgatctgcc gcttgccctg aaatacgcgc aagccatctc cacgcatgcc ttcgggccgg
     3181 aggtgccgca ctgggcgacg cagatgcgca ttttcctcct cgaagacatg ggcgaagtag
     3241 aaagcgtgaa ggttcttttg ggtgggttac tggtgagcgg ggtcatcacc gaccctcatg
     3301 aaatccattt tctcaccgaa cgtctgcaac agctcgaggc tgcggaaaag tcgtcgaaga
     3361 cgccggaaaa atgacatccg aaatcgaaca caagcttagc tttcgcctgc ctgcccggct
     3421 taattggcct tgacaacaat tttgtatata gccaaactgc gccccaatgt tggcccgggc
     3481 ccctggagca aagcagaaaa tctgggcaaa acgcgaggct gggcatggta tttgctggct
     3541 gagtacgaca cgaagactac cctaagcggt gaagggatgg ccaaaatggt gcgcaaacat
     3601 agcggtttta ctctgattga gatcgccatc gtgctggtaa ttatcggcct gcttttaggc
     3661 ggggtactga aaggccagga actcattacc agcgccagag tgaggaacct tatttccgag
     3721 caggacggga ttaaggcggc attcttcggt ttccaggacc gcttccgctc tttccctggg
     3781 gactacacac tagcatcgac gaatatcaac tgcacgccag cttgctcaaa cggcaacggc
     3841 aacgggcgta ttgaagctac gggcgctacg atagccgaaa atattgcggt ttgggaacac
     3901 ctttcacgat cgggttttat taacggtaca tacacatatg ctgctaccgc atcggacagc
     3961 accagtccca agaatccgta tgcggtatac gtaaacgtgg tgttcgatgc ggcttatggc
     4021 gacccggccg tcgtcacgac tccccagcga cacaatatca agactggtgg tcaagtaccc
     4081 gtcgagatca ttgcggaggt ggatcgcaag gtggatgatg ggcttcctta cagcggcgct
     4141 ttccagttct cgaactacca aggtaacgct gctgccgcgc ctacggttag ttgcgtgaat
     4201 accgcgacga cgccgacttc ctgggcagtc accagcggag tgaccaactg cggcggcgca
     4261 agccttctgt agcaaggaac cagccgccct caaagctcgc ctaacctgcg gacactcgct
     4321 cgtttcggtt ttccacccat acctgactac cggcacacac cctgcgccgg cgaactgtta
     4381 caacgcctcc acttgcgcgt ggattgcacc agcagcgtgg agaccccctg cggcgctagc
     4441 ggcctgtttt aaacaaatac taggctaaaa accaatgaat gtggcataga caaagcggct
     4501 ttactctgat tgaaattgcc atcgtgcttg tgattatcgg tctgctcttg gggggggtgc
     4561 tcaaaggcca ggaattgatt accagcgcaa gggtaagaaa ccttatatct caacagaacg
     4621 ggattaaggc ggcgtttttt ggtttccaag atcgctttcg agctttccca ggggactatg
     4681 cgcaggctac ggtcaacatc agtggtgtgt caacctccgt atgtggcggc ggaaacggca
     4741 acggtaatgg tcgcatcgaa ttcacaaaca gtgaaaatat tctggcatgg gagcatttat
     4801 cgaaagcagg gtttatcaac ggaacctaca cctgcgctgc gaccgaatcc tcgacaacca
     4861 gtccggttag cccttacggg atttacctgc aactgattta cgacggtgtg tacgggataa
     4921 ccagcggtgg agtgccccag cataatctta agacgggggg ccagattccc gtcgaaatca
     4981 tctccgaagt agatcggaaa atcgatgatg gcgtgccata caccggtgcc ttccagttct
     5041 ctgcctatca gggaaatgct gcagccgcac ctactgaagg ctcgactact gcgcccgctt
     5101 gcacgagctc caacaccact gcggcagttt ggaactccac caacggcagt agcaactgcg
     5161 gtggcaccag catcttctag caggtttttt atgcggccaa aaaaacccac ttaatgcagg
     5221 ctgttgcgtg aaaaattaac cctgcagcaa tttgatttct gctgcggcga gattgtcctc
     5281 ggtgcccagc agcaccaaca cgtctcctgc ttcgaggcgc gtctccggcg aaggatccaa
     5341 accgcggata ttgcgccgcc ttaccgccgt cacttccact gcaagctttg ccagattgat
     5401 ttcgcccagg gtcttgccta gcgccgcggc acccgccgcg atcaccaccg acctcagtct
     5461 ctgttgctgc ttttcgccgc cttcgggctc atccgccatg ccgcggaaca aaccgtgaaa
     5521 caggcggtag cgccgctcgc gggtttcgcg aatacgcttc agcacccggt ttaacggcac
     5581 gcccaacaga atcagcgcgt gcgaagcgag catcagcgaa ccttccatta tctccgccac
     5641 gacttcggcc gcgccggcct ctttgagctg ctccaaatcc gcatcgtcga gcgtgcgcac
     5701 cagcaccggc agatccgggc gtgcttcctg aacatggctc aggattttca gcgccagcgg
     5761 cacattcgat tccgccacca ccagggcttt cgcccgcatc agtcccgccg caatcaggac
     5821 ttcgcgccgc ccggcatcgc catacactac gttgtctccc gccgccgcgg cttccctcac
     5881 ccgttgcgga tcgagatcga gcgcaacaaa caggatgttt tcccgctcca gaatgcgcgc
     5941 aaggttctgt ccactgcgcc cataaccgca aataatcaca tggcgctccg ccgccagggt
     6001 ttgcaccgcg atgtgatgca actgcatggc gcggttcatc cattcggcgc ctgagaaatg
     6061 caaagccaga tgttccgccc tttcaataat gaacggcgcc gcaagcatgg acagcagcat
     6121 tgccgccaac accacctgta ggatttccgc gtccagcagt cgcaatcctc ccgcctgcca
     6181 taacagcacg aagccgaatt cccccgcctg cgccagacat aaggcggtgc gcagcgcggt
     6241 gccgggctgg ctgccgaacc cgcgacttaa ccccgcaatc agcatgatct tcgacagcac
     6301 cagcgcgacc agcagaccgc cgaccgccag ccagttttcc agcacgctcc tgacatcgag
     6361 cagcatgccg acggtgacga agaacaggcc cagcagcacg tcgcggaacg gcttgatgtc
     6421 gtcttcgacc tggtaacggt attcagtttc agaaatcagc atgccggcga ggaatgcgcc
     6481 gagcgccagc gacaggccag ccagttgagt gagataggcg agacccaggg taatcagcag
     6541 cacgttgaga acgaacagct cggaggattt ctgtctggcc accagatgaa accacgcgcg
     6601 catgaggcgc tgcccaagga acagcagcaa tgccagcacc acgacggctt tgagcgcggc
     6661 tatccccagc gctgcagcta tgtcgctccc ttccgccaag gccggcacga tgatgagtag
     6721 cggcaccacc gcgaggtctt gaaacagcag cacgccgatc acctgccggc catgctcggt
     6781 ggtgagctcc aggcgctcgg agagcatctt gctcacaatc gcagtggaag acatggcgag
     6841 cgcgccgccc agtgccaagc ctgcgcgcca ttccaggccc atggcgaaac ccgccagcag
     6901 gacgatgagc aaggtcaaga caacttgcgc ggcgcccagc ccaagcacga tgcgccgcat
     6961 ggtaatgagt ttcggcaggc tgaattccaa gccgatgctg aacatcagga acaccacgcc
     7021 gaattcggcg agatggcgcg taccggcgtc ctcatgtatc caagccagcg cgtgcggacc
     7081 gatgaggatg ccgaccagga ggtagcccag cagcggtggc agctttaaca ctcggaacag
     7141 caccaccacc agcactgcgg tggcaagcag aatcagcact agttgcagcg ttgtttgcat
     7201 aaaaattttt cttgtgaact gtaactaaat gatgccgtaa tgcgggtgaa atgcaagcga
     7261 ccacattcag attttgcgcc gcgccgcacg cctgccccgc caaggctgcc gcgacacggt
     7321 gagcgaagct tgggtgcggg agcggcagcg tgagcgcccc gtttaacggt atttattgcg
     7381 gcagcttgcg ccccttcccg ctccaggccc gccgacacag cgttggcgga gccatccgga
     7441 gtgggggcgg gccaatgacg cgcttcccgc aaacagctgg ctttgccagt aacgtgtccg
     7501 cgcgccctgc gcgagtccga gtccgggggc tgtcgcgata cggtgagcga agcttggggt
     7561 gcgggagcgg cagcatgagc gccccgttta acggtattta ttgcggcagc ttgcgcccct
     7621 ccccgctcca cgctgcgcga gtccgagtcc ggggcgcctt ctgctatact ttccacgatg
     7681 agcagtgtga aaaaacccgc aaagtcggct ctgccgcaaa cgctggcgct ggcgcgtcaa
     7741 gtcctggaaa tcgaggcgcg cggcatagtg gcgctgatcg accggctcga ccagcaattc
     7801 ctgcgcgcgg tggaactgat tttgcactgc cgcggcagag ttgtggtaag cgggatcggc
     7861 aaatccggcc acatcgcacg caagatcgcg tctaccctag ccagcactgg gacgccggca
     7921 tttttcgtgc atcccgcaga agccagccac ggcgatctcg gcatggttat gcgcgaggac
     7981 gtgctgatcg cgctttccaa ttccggcgag agtacggagc tcctcaccat tgtacccatc
     8041 ctcaaacgcc agggcgcgaa actgatttcg attaccggca atccccggtc ctcgctggcg
     8101 gttgaagccg atgtgcatct cgacgcgacg gtggccgaag aagcctgccc gctggggctc
     8161 gcgcccaccg ccagcaccac cgcggcgctc gccctcggcg atgcgctggc ggtggcgctg
     8221 ctggacgcgc gaggctttgg catcgaggat tttgcccgct cgcatccggg cggtgcgttg
     8281 ggccggaagc tgctgactca cgtgcgcgac gtgatgcgta tgggtgatgc catgccggtc
     8341 gtatccgaaa acgcaacatt cccccaagcg attctggaaa tgtcgaaaaa aggcatgggc
     8401 atgactgctg tgctcaatac taggcaatcc gtagtcggga tttttacaga tggcgatttg
     8461 cgccgcgccc tggaaaaaat caccgacttg cgcagcgtca tggttgcccg ggtgatgaca
     8521 cgcaacccgc gcaccatcgc ccccgatcgt ctggcggtgg aagcggtgga aatgatggag
     8581 agccacaaaa tcaaccaact cctggtggtg gacgccgacc agcggcttat tggcgcgctc
     8641 aacatgcacg acctgttccg cgccaaagtc atttgatgag ctgttagcca tctgtagtca
     8701 gctttcagct tttgtgactg actgacggct aatggctgat cgctgaaagc taactcatgc
     8761 aagctgtttt cgaaaaagcc aaacgtgtcc gtctcgcgct gttcgacgtg gacggtgtgc
     8821 tcaccgacgg cagtctctat atctccgaca gcggcgagga atacaaggcg ttcaacacaa
     8881 tggacggcca cggcatgcgc atgctcaaag ccagcggagt cgagcttgcc atcattaccg
     8941 gccggacttc gcgctgtgtg gaaatgcgcg cgcaaaatct cggcatcgga ctgctttacc
     9001 aaggcgtgga caacaaactc gaagtctacg attcattgct gaagaaattc gctctctctt
     9061 ctgatgccac ggcttttgtc ggcgacgatg tggtggattt gccggtgatg cgccgcgcag
     9121 gattggccat ctgcgtaccc gaatcgccgg ccttggtaaa acagcatgcg cactacgtca
     9181 cgcaagcagc gggcgggcgc ggtgcggtgc gtgagatctg cgagctcatc atgcaggcgc
     9241 aggagaccta ttcggcgcag atcgccccct atctcaaatg aacaaccggc tgctcatctg
     9301 ggcccccctc ggcatgctgt tcctgctggc cgcgctgacg tggtggctcg acagcaaggt
     9361 gcagccgccc cagcccaagc gtgacggcag cagccgccac gacccggact atatcgttga
     9421 ggatttcacc acgacccgca tgagcaagga cggcatgccg cgctatatcc tccgggcaaa
     9481 aaaaatggtg cattacccgg atgacgacag cacccacctg gactacccgg actttcgcca
     9541 tgtggaaacc ggcaagccgg aactgcgcat cacctcagac cagggcttcc tttcgagcgg
     9601 cggcgaagat gcttacttca ttggcgacgt caaagtggtg cgcgagccgg atgcggaata
     9661 tggagagctc aggctctcta ccagtttttt gcacgttatc cccgaccagg atctcgccaa
     9721 gaccgacaag ccggtgacgc tcaccgaccc gcataccata atcaatgcgg ttggcttaga
     9781 attgaatagc aatacccgcg aactcaagct cctctcccaa gtaagaggcc attatgagaa
     9841 tccaaagtaa accgcggtct cttccgctgt gggcgctgct gggagttctc ttttgcgcgc
     9901 cggcggcggc ggagcgcgcc gaccgcgata agccggtcaa cgtggaagcg gacaaagtca
     9961 atgtggacga cgccaacaaa gtgagcacct ttgagggcaa cgtggtgctc acccagggca
    10021 ccattcgcat ccgcgccgac aaaatgattg tgcgccaaaa tgaagacggc tttaattccg
    10081 gcaccgctta cggcaacctc gccaccttcc gccagaaaat ggaaggtgtc gacaaattca
    10141 ttgagggcga ggcggaacgc atcgaatacg acggcaaggc agagaaactg gaactgttca
    10201 cacgtgccca cgtcaagcgc ggccaggatg aattgcgcgg caactatatt tcctacaacg
    10261 cgcagacgga attctatcag gccttgagcg gcaaaccggc gaccgcttct gaaacgaaac
    10321 cgggccgggt acgcgcagtc atccagccca aggacaagaa caacaaaccc gatacgccgg
    10381 cctcgcccgc aacgctgaaa ccctcttccg gcatcgtcaa tccgccgcaa gaataaaaac
    10441 cttaacattg ctaacgacca cgttaacctt ggcgggcacg attgatgagt gaactcaaag
    10501 cgcagaagct gagaaagcgt tacaagtcac gaaccgtggt gcgcgatgtg tcgctggaag
    10561 tgcgcagcgg cgaagtcatc ggcctgctcg gccccaacgg cgcgggcaag accacctgct
    10621 tttacatgat ggtgggcctg gtgcctttgg acggcggcag tattactctg gatgagcgca
    10681 acctgagcca catgccgatt caccgccgcg cgcgactggg tctgagctat ttgccgcagg
    10741 aagcctcgat cttccgtcgg atgacagtgg cggaaaacat ccaggcggtg ctggaactgc
    10801 aagacttcca gcccgatgaa atcgagaacc ggctagacga tttgctgcac gatctgggca
    10861 tcgcccattt gcgcaacaac ccggccatgt cgctttccgg cggcgagcgg cggcgcgtgg
    10921 aaatcgcccg cgcgcttgca tccaacccgc gcttcatcct gctcgacgaa ccgtttgccg
    10981 gcgtggatcc catcgccgtg ctcgacatcc agaaaatcat cggcttcctc aaggagaaag
    11041 gcataggcgt attaatcacc gaccacaacg tgcgcgagac cctgggaata tgcgaccggg
    11101 cctatatcat caacgacggc tcggttctgg cctacggcaa acccgacgaa atcgtttata
    11161 atgaaagcgt gagaaaagtg tatttgggcg aacatttccg gctgtaaaca ggcgggtacc
    11221 ccccgaccac tcacccgcga caagaaaccc gaaaccgaaa gagaatgaag cacaccctcc
    11281 agctcaagct gtcccagcac ctgacgctga cgccgcagct gcaacaatcc atccggcttt
    11341 tgcaactctc gacactggaa ctcaaccagg agcttgagcg cttcctgcag gaaaatcccc
    11401 tgctcgagcg cgacgacgag gtggcagatg aaacgccgac ggtgtacccg accgggaacg
    11461 acgctttggc agacagcgct tctcctgcga atgaacgccc ggtggaaagc acgcaggaga
    11521 ccacgctcag cgccgccgac atggtggagc ccgtctggtt cgaggaagcg gatttctcca
    11581 gtgcgcgcga ggactccgat gaagcggagt atccgcagct cgcggcagtc tcgccgacac
    11641 tgcgcgagca tctgcactgg cagcttgcgc tcaccaagct cgtcgagcgc gacaataaac
    11701 tggtgtcgct gttgattgat gccctggatg aagacgggtg cttaacgcag ccgctggaag
    11761 agctggttgc gctgttgcgg gaagaattga gcgtgaccct ggatgaactg caaattgcgc
    11821 tgaagcacct gcaaaaattc gaaccggcag gcgtgggcgc gcgcaatctg ggcgaatgcc
    11881 tcaaacttca gctggaagcg ctgcccgagg atactctcta tcgcgccgag gctttgaaaa
    11941 tcgtgacgga acatctggac agcctggcgt cccgcgactt tgccaaactg aaaaaaaatc
    12001 tgcattgcga tgaccatacc ctgcgccagg cgcagcattt gatcaccagc ctgaatccgc
    12061 ggcccggcgc ggattatgcg caagtggaga cgcgctacgt agtggccgac gtagtggtga
    12121 agaaagtcaa aggcatatgg gtggcgaacc tcaacccgga cgcgatgccc aagctcaggc
    12181 tcaaccgcat gtacgccgac atcctgcacc gcaaccgcga ttcgagcttc cagcagctcg
    12241 ctccgcaatt gcaggaggcg aaatggctga tcaagaacgt gcagcagcgc ttcgacacca
    12301 ttttgcgcgt ctcccaggcc atcgtcgacc gccagcgcca tttcttcgag catggcgaag
    12361 tggcgatgcg ccctctcgtg ttgcgcgaaa tcgccgaagc cttgagtttg catgaatcca
    12421 ccgtctcgcg cgtcaccaca cagaaataca tgcttacccc gcgcggcatt tacgaactca
    12481 aatacttttt cggcagccat gtcgctactg agaccggcgg tgcctgctcc gccaccgcca
    12541 ttcgcgcgct gatcaagcaa ctggtcgccg ccgaagagcc gaaaagaccg ctttccgaca
    12601 gccagatttc ggaaattctc ggccaacagg gcatcgtggt ggcgcgtcgc accatcgcca
    12661 aataccgcga agcgctgcag atcccgcccg tcagccagcg caaatcgttg tgatctccaa
    12721 ggaggccgaa tgaacctcaa tcttactgga caccatctcg aaatcacgcc ttcgatccgc
    12781 gactacattg tgtccaagct cgacagaatc acacgtcatt tcgaccatgt gattgatgtc
    12841 aacgtgatcc tttcggtgga gaagctgaaa cagaaaatcg aagtcagtgt gcatgtgcgc
    12901 ggcaaagaca tttttgccga atgcgacaac gccgacatgt acgccgccat cgacctcttg
    12961 gtggacaagc tcgatcggca aatcctcaag cacaaggaaa aacattccga gcaccgtcac
    13021 aacggggcac tcaaacacca gtctcacgat tgatttcatg caattgattc catgcaactg
    13081 atcccgagcc gtcgcggcgg cgcttccggc gataccgcgg acgctgaaaa agccagcctg
    13141 gtcgccaggc tgcttccgcc cgctaacgtc gtcctcgacc tggatgtggc aagcaagaag
    13201 cgtgtgttcg agcaggcagg attgctgttc gagaacaacc acggcatcgc caggagccag
    13261 gtcttcgatg cgttgtttgc gcgcgaaaaa ctcggctcca cggggctggg ccacggcatc
    13321 gccattcccc acggtcgcat caagtcgttg aaagaagcgc tgggggcatt tttccgcacc
    13381 aaagcgccgg ttcccttcga cgcgcctgac ggcttgccgg tcaacctcgt tttcgtgctg
    13441 ctggtgccgg agcgcgccac cgatttgcac ttgcagatcc tgagcgaact cgcccagatg
    13501 ttcagcgacc gcaacctgcg cgagacgctc gctgcctgca gcgatcccgt gctgcttcat
    13561 caactcttaa gcggatggca accccatgcc ccaggtcagc gcagccctgc tgtttcaaga
    13621 taaccaggag cggctcgggc tcacctggat tgccggcgaa gaaaaccgcc tgctcaccag
    13681 cgaggacatc gcgaattcgt ccaatggcgt ggtcggccat cttaatttca tccaccccaa
    13741 ctgggtacag gtgctggacc gcgccgaaat cgaatatttg aaccggctcg atgccaaaac
    13801 catgcaacag tcgctgcaag gcttggcggt cagcaatcct ttttgtttca ttctcgctcg
    13861 ggaagcccgc gcgccggcgg cgctgaaaaa gctcaccgat gaaaaacgca tcccgctgat
    13921 cgcctccacc cagggcagcc tgtatctaat gaatgtgctg cgcgtctatc tttccaaggt
    13981 gctggcggaa atgaccaatt tgcatggcgt gttcctcgat gtgctgggga tgggcgtgct
    14041 gctcaccggc gaaagcggcg tcggcaaaag cgagcttgcg ctcgagctga tcagccgcgg
    14101 gcatggcctg gttgcggatg acagcgtcga gctttatctc atcgcgccgg acacgctgga
    14161 gggacgctgc ccgccgctgc tcagggactt tctcgaagtg cggggattgg gcgtgctcaa
    14221 catccgcacc atcttcggcg aaaccgcggt gcggcgcaaa atgaacctga agctgattgc
    14281 gaacctggaa aaacccagcg gcacgcacat tccggggctg gagcgcctgc ctctcggcgc
    14341 aagctcggag gacatcctca gcgtgaaaat ccgcaaggtg acgattcccg tcgccgaggg
    14401 gcgcaatctg gcggtgctgg tggaagccgc ggtgcgcaac tacattttgc agttgcgtgg
    14461 catagacagc acccaggaat tcatcaagcg tcacgagcaa atgatggccg atccacccgg
    14521 cggcgaagag taaccggcat tgcccgtcat gcagctcatc ctcattaccg gcctttccgg
    14581 ctccggcaaa agcatcgcgc tcaatgtgct ggaagacagc ggctattact gcgtggacaa
    14641 cctgccggcg aagctccttc ccgagatcac ccgctttctc cgggaggccg gccacgaccg
    14701 cgtcgcggtg agcattgatg tgagaagcgg cgatacgttt aaccagctgc cgcagcaaat
    14761 caacgcgctg cgcagccagg gcgtcgacgt gcgcccgctt ttcctcgaag caaaaaatga
    14821 aacgctggtg aagcgttttg ccgaaacccg ccgccgccac ccgctgcagg acgacacacg
    14881 cacgctggcc gaatgcatag cgctggagcg tgagcggctc gccgacatcg gcgatctggc
    14941 gcagcacatc gataccagcg acctaaaccc caacgcgctg cgcggctgga tcaaggatct
    15001 ggtgaaaatt gaccgctcgc gcctgacgct cctgttccag tccttcggtt tcaagaacgg
    15061 cattccgctg gatgccgact tggtattcga cgtgcgctgt ctgcccaacc cgcactatga
    15121 tccggtgctg cgcgaactca ccggtaagga cccggcggtc gttgaattcc tgcacaaaga
    15181 caaaaacgtg ctggacatgc tggccgacat ccagcgcttc atcgaaaact ggctgccctg
    15241 cttcattcgc gacaaccgtt atcacctcac tgtggcgctg ggctgcaccg gcggccgcca
    15301 ccgctcggta tatttcgccg aaaagctcgc cgcacatttt cgaagcaaga accaggtgct
    15361 ggtgcgccac cgcgaactcg cctgaggcca tgaacctgtt tcgctacacc aaaccgggag
    15421 actggttggt attcagctgc ggcttgctcc tgtgcctggg cttgatccta actttgtggc
    15481 ggcacgatgc gccgcagcgc gccatcatcc gcagcggcgg caaaatcttc gctgaagtgc
    15541 ggctccctgc caaccaatcc ttcgccatct ccggccctct gggcacgact ttcgtcagca
    15601 tccgtaatct cgaggcgcgc atcagttcgg accccagccc acgtcaatac tgcgtcaagc
    15661 aaggctggct gaaaagcgcc ggcgaaaccg ccgtgtgcct gccgaatcag gtgagccttg
    15721 agcttctggg ccgcaatacg agttatgact cgctcaacta ttgagttgat ggctttccct
    15781 gaagaccatc gcatcgcgaa aatggcagcg tacgcgctcg cgctgactct gctcgaaacc
    15841 ggcattccct cgcctctgcc cggcgtcaaa cccgggcttg ccaacatcgt caccctggtc
    15901 gtactggcgc gctatggtct gcgcttcgcg gtctgggtgt cgttgctgcg tgtggtatgc
    15961 gcgagcctgc tcatcggcac ttttctctcg ccttcatttt tcctgagctt gagcggcgca
    16021 atcgccagcc tcgttatgct ggcctgcacg cgcgttttgc cctgccgatt attcggcccg
    16081 gtaagccaca gcatcctggc cgctttggcg cacatcgctg gacagcttgc cgtcgtttat
    16141 ctttggctga ttcctcaccc aggtatcttc tatctcgctc cggtctttgc ggcggcggcg
    16201 ctgatgttcg gcacggtaaa cggtttggtt gccgcaaaat ggctggaatc cgcaaacgac
    16261 gagccgcgac tggccggcgc cgcgcaacaa atctaggacg cgaatttgag cggcctgctg
    16321 cgctgctcta caataaatga atgaccccac tgccgcgcct ttttccatga tttccatgaa
    16381 aactgttacg ctggctttca ccggcgcatc cggcatgcct tatggcgtgc gcctgctcga
    16441 atcgctgctc gccggcaaga tgcgcgtgta tctgctctat tcgcctgcgg cgcaaatcgt
    16501 ggcgcagcag gaaatggggc ttgctctccc atccagggca aaggaagcac aaagcttttt
    16561 ccgcgaaaaa tatggcgtgg atgaaaatgc gctcaaagtg ttcgggcgcg aggaatggtt
    16621 tgcgccggtg gcctccggct cgaatccggc agacgccatg ataatctgcc cgtgcagcat
    16681 gggcacgctt gccgccggcc tgagcgacaa cctgatcgag cgcgcggctg acgtgacgct
    16741 gaaggaggcc cgcaaactga ttctggtgcc gcgcgagacg ccgctttcgg ccatccacct
    16801 cgagaacatg ctgaagcttt cccgcgccgg cgcggtgatt ctgccggcca atcccggctt
    16861 ctatcaccac ccgcagaata tccaagattt ggtggacttc gtggtggcgc gcattctcga
    16921 ccatctcggc attccgcatc aacttgcagc gcgctgggga aaagaggcga actgaactat
    16981 gattcgagct tttccgtgaa cctcggcctc ttttcaaaga gcgcgccagc gctggaaacc
    17041 ctgccgattt tcccgctcaa taccgtccta ttccccggcg gcatcctcat gctcaagatt
    17101 ttcgagccgc gctacatgga catgagcaag gtgtgcttgc gcgacaacaa accgttcggc
    17161 gtctgcctga tcaaggaggg caatgaggtc ggcgcgcccg cgatgcccga ggaaaccggc
    17221 tgcaccgccg ggatcaccga ttgggacatg cagcaactcg gcgtcttgca tcttaagact
    17281 gaaggcagac aacgcttccg cattcttggc cggcaggtgg caaaagacgg cttgatcagc
    17341 gccgaggtgg cgatgctcgg agcggagccg gagaccccgc ttgcttcgga cctgcgccaa
    17401 tgcgccaccg tgctgaagct catcatggaa aaaatcggcg aggaacattt tcctgcgcca
    17461 gctaagctcg atgatgcaac ctgggtcggc taccgcctcg ccgaagtgct gccgctcaaa
    17521 cttgcggcga aacaaaaaat gctggagatc aacgattcca tcgtgcgcct gcaggtcttg
    17581 cacaagttcc tcgtccagca aggcttggtc gcttaggcgg cctggtcatt caccccgtga
    17641 taacgccaac tccggtcgcc cgccgtattt accgtaaaac catttgagtg caaacggcgg
    17701 cagcgcggtg gtaagcgcaa ccacgatgag taacgcggcg tagagttcgg cgttgagaat
    17761 gccgttgtta agccccacct gggcaaaaat cagcccc
//
LOCUS       DATIOW010000097         3699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1571730, whole genome shotgun sequence.
ACCESSION   DATIOW010000097 DATIOW010000000
VERSION     DATIOW010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571730"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /gene="gshA"
                     /locus_tag="VLL03_03075"
     CDS             complement(<1..677)
                     /gene="gshA"
                     /locus_tag="VLL03_03075"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HSS46381.1"
                     /translation="MAVPHLTAALPGQAPELEIKLVEKVSAIEHWLRGQWQEHSVPFY
                     ASVDLRNSGFKLAPVDTNLFPGGFNNLNPDFMPLCVQAAMTAVEKICPDAKGALIIPE
                     NHTRNTFYLQNVAMLASILRQAGMNVRVGSLLPEITAPTDMKLPDGGSLTLEPLVRKN
                     NRLGLADFDPCVVVLNNDLSAGVPDILKNLEQALLPPLHAGWATRRKSNHFAYYNKVV
                     EEFSRLV"
     gene            complement(802..2163)
                     /locus_tag="VLL03_03080"
     CDS             complement(802..2163)
                     /locus_tag="VLL03_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HSS46382.1"
                     /translation="MSFSGMSFAEEQKAPAAPAAAAAPAAAPAAPAAPTAPVVVDVAK
                     INSGDTAWMLASTALVLMMTIPGLALFYGGMVRKKNVLATVMQSFAVTCLVTVVWMVI
                     AYSLAFTPGSTPYFGSLSRLFLNGMIFQNGSKIAVSHLGLTVPESVYMCFQMTFAIIT
                     PALICGSFADRMKFSAMLWFMGIWQVVVYATIAHWVWEPSGWLAAMGVLDFAGGTVVH
                     INSGIAGLAAALVLGKRVGYGKEAMPPHNLVLTVIGASLLWVGWFGFNAGSAVAADGR
                     AGMAMAVTQIATATAALSWMFAEWIVKGKPSVLGIASGAVAGLVAITPASGFVAPIGS
                     LWIGVAAGVFCFFAATTVKNMLGYDDSLDAFGVHCIGGFTGAILTGVFAVKRIGGTAG
                     VLEGNFGQLLIQFIGASSTLIFSFVMSFIIFKVLDMVMGIRVTEEQEREGLDIALHGE
                     QVL"
     gene            complement(2213..2551)
                     /gene="glnK"
                     /locus_tag="VLL03_03085"
     CDS             complement(2213..2551)
                     /gene="glnK"
                     /locus_tag="VLL03_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HSS46383.1"
                     /translation="MKLVTAIIKPFKLDEVRETLSNIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKVEAAIKSEMLERVIEAIEKAANTGKIGDGKIFVFDLEQVIRI
                     RTGETGAEAL"
     gene            complement(2589..>3699)
                     /locus_tag="VLL03_03090"
     CDS             complement(2589..>3699)
                     /locus_tag="VLL03_03090"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HSS46384.1"
                     /translation="IPIPALDDVLEAWGLSLNGYLDVSYSYLSTSGKFAPPPATPASL
                     NSRVSDFQHNSFSVHQAAFTLAKQPKEGFGALVNLTAGEDADVIHSFGSGFGTTTNFD
                     VTQAFAQYAKGPFTAIGGKYVTLAGAEVINPTQDTNFSRSILFGFAIPFTHTGVRGTY
                     AVSDTLSFILGVNNGWDQLQDANSSKTAEVGVSYAPLKNLSIAAQGYFGREPVTAPLP
                     STQQGFRSLVDVVATYTATDKLTLILNGDWGAQKNTAGSGLVTTGNNTARWYGLAAYG
                     IYQFTDQWRGSLRAEYFGDKDGYRTGVAQDWKEVTLTLAYLPTKNIELRGEVRADRSN
                     GTLSTGGPVSGAFVKTNGSLSKNQTSVGLEALFKF"
BASE COUNT          820 a         1057 c         1084 g          738 t
ORIGIN      
        1 ttgacgagcc tggaaaattc ctcaaccacc ttgttgtaat acgcgaaatg gttggatttg
       61 cgccgcgtcg cccagccggc gtgcagcggc ggcagcaggg cctgctcaag atttttcaga
      121 atatctggaa cgcccgccga caagtcattg ttgagcacta ccacgcaggg gtcgaagtcg
      181 gcgaggccca gacggttgtt cttgcgcacc agcggctcca gcgtaaggct gccgccgtcg
      241 ggtagtttca tgtcggtagg cgcggtgatt tcgggaagca ggctgccgac acgcacattc
      301 attcccgcct ggcgcaggat ggaggcgagc atggccacgt tttgcagata aaaggtgttg
      361 cgtgtgtggt tttcgggaat aatcaacgct cccttggcgt cgggacagat tttttctacc
      421 gccgtcatcg ccgcctgcac gcacagcggc atgaaatcgg gattgaggtt gttgaagccg
      481 ccgggaaaga gattggtgtc caccggcgcg agcttgaaac cgctgttgcg caagtccacc
      541 gaggcgtaaa acggcacgct atgctcttgc cattggccgc gcagccaatg ctcgatggcg
      601 gaaaccttct ccaccagttt tatttccagt tcgggcgcct gcccgggaag cgcagcggtt
      661 aaatggggaa cggccatggg gaatgcagtc ttgaaaaggg aaaggctatt gtaacggaaa
      721 gcgcaaggcc gcgtcaggaa ccgcaggcga aaaaaggggg gcgcttgcgc gccccttcag
      781 gaggactatt tgttatgacg tttacagcac ttgctcgccg tgcaacgcga tgtccagccc
      841 ttcgcgttcc tgctcttcgg tgacccttat acccatcacc atatcgagca ccttgaagat
      901 gataaagctc atgacgaagc tgaaaataag agtcgaagaa gcgccgatga actggatgag
      961 caattgcccg aaattccctt ccagcacgcc ggcggtgccg ccgatgcgct tcacggcgaa
     1021 cacgccggtg agaatcgcgc cggtaaagcc gcctatacag tgcaccccga atgcatcgag
     1081 cgagtcatca tagccgagca tattcttgac cgtggttgca gcaaaaaagc agaatacacc
     1141 agcggcgacg ccgatccaca gagagccgat gggggcgacg aagcctgaag ccggtgtgat
     1201 ggccacgagc cccgctactg cacctgaagc gatgccaaga acggagggtt tgcctttgac
     1261 gatccattcg gcaaacatcc aggagagcgc ggctgtggcg gtcgcgattt gggtcaccgc
     1321 catcgccatg ccggcgcggc catcggccgc caccgcagaa ccggcgttga agccaaacca
     1381 ccccacccac agcaaagagg cgccgatgac ggtgagcacc aggttatgcg gcggcatcgc
     1441 ttccttgccg taacctacgc gtttgcccaa caccagtgcc gcggcgaggc ccgcgatgcc
     1501 ggaattgata tgcaccaccg tgccgcccgc gaagtcgagc acgcccatgg cggcgagcca
     1561 gcccgaaggc tcccacaccc agtgcgcgat ggtcgcatac accaccacct gccaaatgcc
     1621 catgaaccac agcatggccg agaatttcat gcggtcggcg aacgagccgc aaatcagcgc
     1681 cggggtgata atggcgaacg tcatttggaa gcacatgtac accgattcgg gaacggtaag
     1741 tcccaagtgc gagaccgcga tcttcgagcc gttctggaat atcatgccgt tcaggaacag
     1801 gcggcttaaa ctgccgaaat aaggggtact gcccggcgta aaggcgaggc tgtaggcgat
     1861 aaccatccac actacagtca ccagacaggt gacggcaaaa ctctgcatca cggtagccag
     1921 tacgtttttc ttgcgcacca tgccgccgta aaacagcgcc agacccggaa tggtcatcat
     1981 cagcaccagc gcggtcgaag ccagcatcca cgccgtgtcg ccggagttga tcttggcgac
     2041 gtcgaccacc actggagcgg tgggcgcagc aggagcggca ggcgcggcag ccggagcagc
     2101 tgcggcagct ggagcagcag gtgctttttg ctcttcagcg aatgacatgc cgctgaaact
     2161 catcgcggca aacagagcca gagcggtaaa taaacgtttc atgatgctct ccttatagcg
     2221 cctccgcgcc ggtctcgccg gtgcggatgc ggatgacttg ctccaaatcg aagacgaaaa
     2281 tcttgccgtc gccgatcttt ccggtattgg cggctttttc tatcgcctcg atgacccgtt
     2341 cgagcatctc gcttttaatc gcggcctcga ctttcacctt gggcagaaaa tccaccacgt
     2401 attccgcacc ccgatacagc tcggtgtgcc ccttttgccg cccgaagccc ttgacttcgg
     2461 tgacggtgat cccctgcacg ccgatgttgg acagggtttc acgcacctcg tcaagcttga
     2521 acggcttgat gatggctgtg actaatttca tgttgactcc ttaaataatg aataggtgtt
     2581 ggtgctgctt aaaacttgaa cagcgcttcc aagccgaccg acgtttgatt cttgctcagg
     2641 ctgccgttgg ttttcacgaa cgccccactt acaggaccac ctgtagaaag ggttccattc
     2701 gaacggtcag cccgcacttc gccgcgcagt tcgatgtttt tggtcggaag ataagccaga
     2761 gtcagagtga cttctttcca gtcctgtgcc acaccggtgc ggtagccgtc tttgtcacca
     2821 aaatactccg cccgcagcga gccgcgccat tggtcggtga attgataaat gccataggct
     2881 gcaagcccat accatctggc ggtgttgttg ccggtagtta ccaagccgga gccagcagtg
     2941 ttcttctgcg cgccccagtc gccattgagg atcagggtca gcttatcggt ggcggtataa
     3001 gtcgcaacca catccaccag gctgcgaaaa ccttgttggg tggaaggaag aggtgccgtc
     3061 accggttccc tgccgaaata gccttgagcg gcgatggaaa ggtttttcaa cggcgcgtaa
     3121 gaaacgccaa cttcagcggt ttttgagctg ttcgcatcct gcaactggtc ccagccgtta
     3181 ttcacgccca aaatgaagct gagcgtgtcg ctgaccgcat aggttccgcg cacgccggta
     3241 tgggtgaaag gaatcgcaaa gccgaatagg attgaacgcg agaagttggt atcttgcgtg
     3301 gggttgataa cctcggcgcc ggccagcgtc acatacttgc cgccgatcgc ggtgaatggc
     3361 cccttggcgt attgcgcaaa agcttgcgtg acgtcgaagt tggtggtggt gccaaacccg
     3421 ctcccaaaag agtgaatgac gtcggcatcc tcgccggcgg tcaggttaac cagcgcgcca
     3481 aagccttctt tcggctgttt ggcgagggtg aacgccgcct ggtgcacact gaacgagttg
     3541 tgctgaaaat cagatacccg gctatttaac gaagcggggg tggctggggg tggcgcaaat
     3601 ttgccgctgg tagagagata cgagtaagaa acatcaaggt agccgttaag cgagagcccc
     3661 caggcttcta aaacgtcgtc gagcgcggga atcggaatt
//
LOCUS       DATIOW010000098        22931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1875648, whole genome shotgun sequence.
ACCESSION   DATIOW010000098 DATIOW010000000
VERSION     DATIOW010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 22931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22931
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875648"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(<1..143)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            371..571
                     /locus_tag="VLL03_03095"
     CDS             371..571
                     /locus_tag="VLL03_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46385.1"
                     /translation="MEAELKALEEKIQQVAQVCLHLRTDNQHLRQQLATSVNENKQLT
                     GKIDSAKKRLEALLRQIPENEK"
     gene            568..879
                     /locus_tag="VLL03_03100"
     CDS             568..879
                     /locus_tag="VLL03_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HSS46386.1"
                     /translation="MSADSKGVDISIMGRKFRVACAENEQKELLEAVAYLDRKMREIR
                     DQGKVVGLERIAIMAALNVTYELLTLHLADGFDIAEFKRRMTHMQAALDRALTEQEKL
                     F"
     gene            903..1079
                     /gene="ssrS"
                     /locus_tag="VLL03_03105"
     ncRNA           903..1079
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VLL03_03105"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            1138..1599
                     /locus_tag="VLL03_03110"
     CDS             1138..1599
                     /locus_tag="VLL03_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HSS46387.1"
                     /translation="MRYWLMKSEPSEFGIDKLAAAPGKTTPWFGVRNYQARNFMRDQM
                     RLGDLAFFYHSSCKEPGITGIVEVCKPAYPDATQYDRKSCYHDPKATPQNPRWFNVDV
                     KLVNKTRLIRIKELRRHPQLKTMRVLQKGNRLSITPVDPREWEFITRVLLK"
     gene            1640..2434
                     /locus_tag="VLL03_03115"
     CDS             1640..2434
                     /locus_tag="VLL03_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HSS46388.1"
                     /translation="MEWWAAYLAVGAVAGFLAGLLGVGGGLIFVPSLIVLFNLQHFPQ
                     TNLLHLALGTSLASITFTSVSSLRAHHAHGAVNWQIVRRTTPGIVVGTLLGSGLAALL
                     TSSILALFFAVFVSLAATQILLDIKPKPSRQLPGRAGTFFVGIIIGILFSLVGGGGAI
                     LSVPFMLYCNVKTHDAIGTSSAIGFPIAVSGAAGYILSGMAQKSLPPLSLGFVYLPAV
                     LWLVIASVLTAPLGAKLAHRLPVDKLKKILAVLLYIIAAKMLVGLL"
     gene            complement(2441..3397)
                     /locus_tag="VLL03_03120"
     CDS             complement(2441..3397)
                     /locus_tag="VLL03_03120"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HSS46389.1"
                     /translation="MPNNEIEKAVAILRAGGLVAFPTETVYGLGADASNPEAVKKIFI
                     AKGRPQDHPVIVHLPDSSHLERWAGEIPGAARELARRFWPGPLTLILRRAPRVPDAVT
                     GGQDTVGLRVPAHPVAQALLQTFGGGIAAPSANRFGRLSPTTAEHVRAELGDRIDGIL
                     DGGSCTVGIESTILDVSSEHPVLLRPGKITAAEIEAILGTTLRKPEASGTRAPGMLAS
                     HYAPQTPLLVTRNLAECIRSLYRQNQRVAVLAYQSPPDGGLQMVWNAAPVKAADYARE
                     LYANLRKLDQSGCDVIVVEEPPLGEEWAAVRDRLSKAAAKKT"
     gene            complement(3562..4458)
                     /locus_tag="VLL03_03125"
     CDS             complement(3562..4458)
                     /locus_tag="VLL03_03125"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HSS46390.1"
                     /translation="MNRTDNLLFQSDLGSIPFLHRGKVRDIYAVGEDKLLIVQTDRLS
                     AFDVVLPTPVPGKGKVLTALSNFWFQKLGHVIPNHLTGVAPEAVVKEAREREQVAGRA
                     FVVRRLKPLPIEAIVRGYIVGSGWKEYQKSGTICGIPLPAGLKEAQKLLQPIFTPSTK
                     APAGSHDENISFAEAEKLLGKDIAARVRDASIELYSEAAEYAAKKGIIIADTKFEFGT
                     DKQGKLYLIDEVLTPDSSRFWPASHYRTGMSPPSFDKQYVRDWLEAQRWNKKPPAPAL
                     PADVLAKTAEKYHEALRLLAGN"
     gene            complement(4487..4975)
                     /gene="purE"
                     /locus_tag="VLL03_03130"
     CDS             complement(4487..4975)
                     /gene="purE"
                     /locus_tag="VLL03_03130"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HSS46391.1"
                     /translation="MTKKTLVSVLMGSRSDWEVMQHAAKQLEDFGVSYEARVISAHRS
                     PGLLDEYVKSADKTGVKCFIAGAGGAAHLAGVIAAKTTLPVLGVPMPSKHLQGLDSLL
                     SIVQMPKGVPVATFSIGESGAANAGLFAVAMLAVEDTKLAKQLQDFRKKQAKAIKTAR
                     LP"
     gene            complement(5040..5990)
                     /gene="cbbX"
                     /locus_tag="VLL03_03135"
     CDS             complement(5040..5990)
                     /gene="cbbX"
                     /locus_tag="VLL03_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbX protein"
                     /protein_id="HSS46392.1"
                     /translation="MSADPGIPQNLPDDKPVDLEAAFRDSHVKEVLDQLDRELIGLKP
                     VKTRIREIAALLLVDRLRKELGLTSGAPSLHMSFTGNPGTGKTTVALRMAEILHRLGY
                     VRKGHLVAVTRDDLVGQYIGHTAPKTKEVLKKAMGGVLFIDEAYYLYRVDNERDYGQE
                     TIEILLQVMENQRDDLVVVLAGYKDRMDKFFQSNPGLSSRIAHHLDFPDYTAEELLAI
                     ARLMLEASQYRFSADADKAFQDYIVKRRQMPHFANARSVRNALDRARLRQANRLFARN
                     QGKLTKKDLVTIEAEDILASRVFKEGVPDEKASAKPGAAK"
     gene            complement(5990..6412)
                     /locus_tag="VLL03_03140"
     CDS             complement(5990..6412)
                     /locus_tag="VLL03_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HSS46393.1"
                     /translation="MRITQGTFSFLPDFSDEEIRAQVEYCLKNNWAVSVEFTDDPHPR
                     NTYWEMWGLPMFDLKDAAGILYEINQGRKAYPNHYIRVNAFDPTHGWETVRLSFIVNR
                     PKNEPGFQLERQEGPGRRIHYTIRSYATNQPKGERYQD"
     gene            complement(6424..7884)
                     /locus_tag="VLL03_03145"
     CDS             complement(6424..7884)
                     /locus_tag="VLL03_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="form I ribulose bisphosphate carboxylase large
                     subunit"
                     /protein_id="HSS46394.1"
                     /translation="MGGPDKAPKGKERYKSGVIPYKKMGYWDAGYVPKDTDVITLFRI
                     TPQEGVDPEEAAAAVAGESSTATWTVVWTDRLTACEMYRAKAYRVDPVPNNPGQYFAY
                     IAYGLDLFEPGSIANLTASIIGNVFGFKAIKALRLEDMRLPVAFVKTFDGPPTGIVVE
                     RERLDKFGRPLLGATTKPKLGLSGKNYGRVVYEALKGGLDFVKDDENINSQPFMHWRD
                     RFLYCMEAVNRACAETGEVKGHYLNITAATMEDMYERAEFAKELGSIIVMIDLIIGYT
                     AIQSISKWARRNDMILHLHRAGHSTYTRQKNHGVSFRVISKWMRLAGVDHIHAGTVVG
                     KLEGDPNMVQGYYNILREPRNEVDLQRGIFFEQDWAGLRKVMPVASGGIHAGQMHQLI
                     HYLGEDCVLQFGGGTIGHPMGIQAGATANRVALEAIIQARNEGKDIWNEGPRILEKAA
                     KFCAPLKSALEVWKDITFDYASTDTADFVPTPSVSA"
     gene            8023..8937
                     /locus_tag="VLL03_03150"
     CDS             8023..8937
                     /locus_tag="VLL03_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HSS46395.1"
                     /translation="MLHLTLRQLRVFEAVARHLNFSRAAEELHLSQPAVSMQIKQLEE
                     NAGLPLFEQMGKRIYLTEAGQEMFQYSRSVAQQLQEMEAVLDQMKGLERGKLNISVVS
                     TANYFAPQLLAKFCQQYNKVAVSLSVSNREVVLNQLADNEADLAIMGQPPEGLDIVAE
                     SFMENPLVVIAPPNHRLARQRKIPRARLQQETFLLRETGSGTRSAMERFFVEHRIKLN
                     TGMEMGTNEAIKQAVQAGMGLGIISLHTIELELETKRLKVLDVESFPIKRHWYVVHRR
                     QKRLTKAAQAFKEFLLKEAPKLMRKTRA"
     gene            complement(8981..10009)
                     /locus_tag="VLL03_03155"
     CDS             complement(8981..10009)
                     /locus_tag="VLL03_03155"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HSS46396.1"
                     /translation="MYAQQLENAAAAMVAKGKGILAIDESSSTCKKRFDSIGVESTTE
                     NRRAYRELLITVPGGGQYVSGMILFDETIRQATADGTPFPEALKRQGILIGIKVDTGA
                     KKLAGAPGEQVTEGLDGLRERLVEYRGLGASFAKWRAVINIGEGIPSQYCLDANAHAL
                     ARYSALCQEAGLVPIVEPEVIMEGNHTIERCFDVTEATLRTVFNALYQQRATLEHMIL
                     KASMVISGDKCPKQASVEEVAKQTVRCLKRAVPAAVPGVVFLSGGQSAEVATAHLNVM
                     NQMGPHSWPLSFSYGRALQDPALKTWRGQAGNVAAAQKALYHREKMNSAACYGRYTAQ
                     MEREKAAA"
     gene            complement(10249..11682)
                     /gene="pyk"
                     /locus_tag="VLL03_03160"
     CDS             complement(10249..11682)
                     /gene="pyk"
                     /locus_tag="VLL03_03160"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HSS46397.1"
                     /translation="MLRRTKIVATLGPASNDPKIMERMITAGVDVVRMNFSHGTAQDH
                     LSRAETVREAARKVGRTVGIIADLQGPKIRIGQFEQGKITLNSGDTFILDADGALGNQ
                     ERVGLDYRELPRDVAEGSVLLLDDGRIVLEVQAVRGNEIICKVRHGGVLSNNKGINRQ
                     GGGLTAPALTAKDMEDIKTAVMLKADYLAVSFPKSGADMRIARELLRNINSQALLIAK
                     IERAEAVPMLQDIMEACDGIMVARGDLAVEVGDAAVPALQKRMIRMAREMNKFAITAT
                     QMMESMIESPIPTRAEVSDVANAVLDGTDAVMLSAETASGKYPVQTVEAMSRICLEAE
                     KSTEVTLDREFLDRVFTRVDQSIAMAALFTAYHLKVKAIAALTQSGSTALWMSRLNCG
                     VPIYALTSEASTRYRVSLFRDTYPLMVQYLGQDREELLREAEKVLLANGVVREGDLIV
                     LTIGEPIGESGGTNTMKIVKVGAQRQK"
     gene            complement(11755..12936)
                     /locus_tag="VLL03_03165"
     CDS             complement(11755..12936)
                     /locus_tag="VLL03_03165"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003269405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HSS46398.1"
                     /translation="MRVLKMTDLDLAGKRVLIREDLNVPLKNGEVFDDTRIRASIPTI
                     EHALKNRARVMLMSHLGRPEEGKYDEANSLKPVARRLSQLLKREVKLLNNWLNGVEVA
                     PGEAVLFENVRFNKGEKKNGDELARRMAGLCDVYVMDAFGTAHRAEASTHGVAKFAKV
                     ACAGPLLAAELEALGRALENPKRPQVAIVGGSKVSTKLTALKSLSQVVDQLIVGGGIA
                     NTFMLAVGLPIGKSLADAGMVDEARGIINAARAKGGDVPIPVDVVCAKEFSEAAHATL
                     KDAEEVADDDLIMDIGPKTSAKLASVLEKAGTILWNGPLGVFEFDQFGEGTRALALAI
                     AKSKAFSIAAGGDTLAAIAKYGVIDQISYISTGGGAFLEFLEGKKLPAVEILEQRAAK
                     Q"
     gene            complement(12966..13979)
                     /gene="gap"
                     /locus_tag="VLL03_03170"
     CDS             complement(12966..13979)
                     /gene="gap"
                     /locus_tag="VLL03_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HSS46399.1"
                     /translation="MAIRVAINGYGRIGRNILRALYESGRHQEIQIVAINDLGNAETN
                     AHLTRYDTAHGRFHGTVQVDGDSMVVNGDRIKVLAVRNPTELPWKDLNIDVVHECTGL
                     FTSKGKASAHLKAGAKKVIISAPAGKDVDATVVYGVNHKILKPSHTVISNASCTTNCL
                     ALLAKVLHEKIGIVSGLMTTVHAYTNDQVLTDVYHEDLRRARSATMSMIPTKTGAAAA
                     LGLVLPELNGKLDGFAVRIPTINVSLVDLSFIAKRPTSTEEINRVMKEAAQGELKGLL
                     NYNDKPLVSVDFNHDPASSIFDATLTKVSGGTMVKVLSWYDNEWGFSNRMLDVTVAWM
                     NAK"
     gene            complement(14050..16041)
                     /gene="tkt"
                     /locus_tag="VLL03_03175"
     CDS             complement(14050..16041)
                     /gene="tkt"
                     /locus_tag="VLL03_03175"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HSS46400.1"
                     /translation="MATRKDLTNAIRALAMDAVQKANSGHPGMPMGMAEIAEVLWNHH
                     LRHNPANPKWPDRDRFVLSNGHGAMLQYALLHLTGYDLPMEELKRFRQLHSKTPGHPE
                     RGCAPGIETTTGPLGQGLANAVGMAIAEKVLASTFNRPGFDLVNHCTYVFLGDGCLME
                     GISHEACSLAGKLALGKLIAIYDDNGISIDGHVEGWFTDDTPKRFEAYGWHVVPNVDG
                     HNFDAVDAAIKKAKQVTDKPTLVCCKTVIAMGAPNKAGSHDAHGAALGEAEVAATRAN
                     IGWNHPPFEIPKDVYEGWDARGRGAKLDSEWERRFSEYAKQYPREAAEFTRRMNGELP
                     ADFREHVAQLISKANEKAENIATRKASQNAIEGLAPILPELIGGSADLTGSNLTLWSG
                     SRAVDEKDGGNYIYYGVREFGMAAIMNGLALHGGVIPYGGTFLMFSEYARNALRLAAL
                     MKIRTIFVFTHDSIGLGEDGPTHQAVEQAATLRLMPNMDVWRPCDTVESVVAWAQAIE
                     RGNGPSSLLFTRQNLPFQKRDAVSFAHIAKGGYVLAEAPGGKPRAVIIATGSEVRLAV
                     GAQKALAEEGIAVRVVSMPSTSVFDRQDEAYKSSVLPKGVVRVAVEAGVTDGWRKYVG
                     LEGAVVGIDRFGESAPAADLFKYFGFTVETVVKAVKSVV"
     gene            complement(16229..16417)
                     /locus_tag="VLL03_03180"
                     /pseudo
     CDS             complement(16229..16417)
                     /locus_tag="VLL03_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524925.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(16691..17497)
                     /locus_tag="VLL03_03185"
                     /pseudo
     CDS             complement(16691..17497)
                     /locus_tag="VLL03_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016766.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(17510..19633)
                     /locus_tag="VLL03_03190"
     CDS             complement(17510..19633)
                     /locus_tag="VLL03_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS46401.1"
                     /translation="MRYFVILCLGLGAVLLFLLSTASANTALFAQHYPLILALNGAIT
                     LCLIGVVTVQLLALRKKLSAKVFGAKLTLRLVLFFSLMAVLPGALVYAVSVQFLAKSI
                     ESWFDVRVEKALEGGLNLGRTTLDNLLKDLNKKADTMALSLSELPVAEHLTALNTLRE
                     QLGVQEATLFSQRGTLIAFSISEKSGLMPDLPSSALLKQIRQQQAYSGAETIPGKGLY
                     LRVMVPVNVLNVNEGIRVLQLLQPVPRQLAQDAETVQMVYRDYQELSLSRAGLKRLYA
                     ITLTLTLLLAWLSAIALGFLISERMSAPLEMLAEGTRAVAQGDFSQRQPVKSRDELGV
                     LTQSFNLMTHQLAEARMTAERHQEQLQTAKAYLESVLANLSAGVLAFDEGFRLRSANP
                     SAGYILETEFARLRGTRLEEWRAGRAALGVLSQEILQSFQRAPQDGWEKQVEYGGESG
                     IRVLLLRGTRLAGAAQAGYVVVFDDITHLLQAQRDAAWGEVARRLAHEIKNPLTPIQL
                     SAERLQYKLANKLDQRDLEILKRATQTIISQVAAMQNMVDAFSDYARSPELALYPLDL
                     NRLLREVLALYESPALAIKLELAPDLPQVLGDKTQLRQVIHNLMQNAQDALTEVPAPG
                     IVVRTAAVNGRIQLSIQDNGSGFPEQLMTRLFEPHVTSKPRGTGLGLAIVKKIVDEHS
                     GMIHIANVKPHGACVSISLPLAHAA"
     gene            complement(19647..20165)
                     /locus_tag="VLL03_03195"
     CDS             complement(19647..20165)
                     /locus_tag="VLL03_03195"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HSS46402.1"
                     /translation="MLLSLAPAPVVAEGIQVKAAAFGADGKNYLLQADLDIALTHTLE
                     EALNKGVALYFVTEFELSRSRWYWFDQKLVQLKHQYKLSYNALTRQYRLSVGNLFQNF
                     ATLQEAMGLMSQLRDRVAVEKAALEKGKTYVAALRMRLDVSQLPKPFQLNAVASKDWN
                     LSSGWHRWEVTP"
     gene            complement(20221..21480)
                     /gene="rsmB"
                     /locus_tag="VLL03_03200"
     CDS             complement(20221..21480)
                     /gene="rsmB"
                     /locus_tag="VLL03_03200"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HSS46403.1"
                     /translation="MIEAQRLASLAVSQVLEGHNLTSTLSALWRRQPSLGAQQRAQAQ
                     ELAYGTLRYCGEAQALLGLLLEKTLRDDKLRALLLVSLYQLQYGKAAPYAVVDHAVNA
                     AATLNQRAAKGLVNAVLRNFLRHRESLTRQARDNEVGLYSHPQWWIDKLKAQYPHQYQ
                     AILLAANQHPPMTLRVNRRASTKRDYHLRLQSAQIEAVDLGGDALMLKRPLMVEELPG
                     FAEGQVSVQDAAAQRAAPLLDVRPGMRVLDACAAPGGKTAHILEQADAEVTALDNDGE
                     RLRRVEENLRRLKLKARIVCGDAGQPATWWDGVPYQRILADVPCSASGVVRRHPDIKW
                     LRRETDVGKFAGQQSRILDALWQCLDGGGKLLYVTCSVFAEENRQQVDAFLGRHKNAE
                     LQPLNTDGQLLPDSQHDGFFYALLRKI"
     gene            complement(21500..22345)
                     /gene="htpX"
                     /locus_tag="VLL03_03205"
     CDS             complement(21500..22345)
                     /gene="htpX"
                     /locus_tag="VLL03_03205"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007581962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HSS46404.1"
                     /translation="MGNWLKTSLLMAAIMALFGTVGSLLGGGSGMAIALVLGGAMNLW
                     AYWFSDKMVLRLYNAQEVDETRAPELYGIVRELAQRAHLPMPRVYLIDQAQPNAFATG
                     RNPEHAAVAATTGILQLLTPRELRGVLGHELAHVQHHDILISTLSATIAGAISSVAQF
                     GMLFGGGNQNKRGVHPLAALLIMIVAPIAAMLIQLAISRAREFEADRSGAEISGDPGA
                     LASALEKIERFARGETMPVAEMHPETAQMMIINPLSGGGVQGLFSTHPSTQERIARLL
                     AMARA"
     gene            complement(22422..>22931)
                     /locus_tag="VLL03_03210"
     CDS             complement(22422..>22931)
                     /locus_tag="VLL03_03210"
                     /inference="COORDINATES: protein motif:HMM:NF014908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HSS46405.1"
                     /translation="GLDTGPLLLQQRLQITDDDTGQTLHDKLARLGGECIVRALQALE
                     RGELQPQLQPRTGASYAAKVEKTEAEIDWKKEARLIERMVRAFNPHPGASTRLNSEPL
                     KIWKAGVENHVAGNPGEVIAANGQGIVVACGTGGLRIEELQKAGSKRLRAQAFLAGSA
                     LKPGTRLGA"
BASE COUNT         4573 a         6886 c         6465 g         5007 t
ORIGIN      
        1 gcctcacagt ggctaaagtg atacgcccgc gcttacttac cgttgcgggg gcagcacagg
       61 ctttgcgcga gatgcgcgca cctgtttccc gtttaaccca agccgaagaa ttcagcccgg
      121 gcacctgcaa acgaagtgca atcaagccat gcaaatatca actgctcaca tggctttccg
      181 atattttaca tggccaaact caggcagggt aagagtaaat acttacatac gccaacttct
      241 ttgtccctat ttgtttacta aatctgctgt aaggggcaag ccctcagagt tggatggtag
      301 atattaaagt acattcaaaa aatactgtta gcagttgacc ttgcaggatt tttaaaccta
      361 tagtgtattt atggaagcgg aactgaaagc cttggaagaa aaaatccagc aggttgcaca
      421 ggtgtgtctg catttgcgca ccgacaatca acacctgcgt cagcagcttg ccacttccgt
      481 aaatgaaaac aagcagttaa ccggaaaaat cgatagcgca aaaaaacgcc tggaagcgtt
      541 gttgcggcag attcccgaga acgagaaatg agtgccgaca gcaaaggcgt ggatatcagt
      601 atcatgggcc gcaagttccg cgtggcctgt gccgagaatg aacagaagga actgctcgaa
      661 gcagtcgcct atctcgaccg gaaaatgcgt gaaatccgcg accaaggcaa ggtcgtggga
      721 ctggagcgca tcgccatcat ggccgcgctc aacgttacct acgaactcct cacgctgcac
      781 ctcgccgacg gttttgacat cgcagagttt aagcgtagaa tgacccacat gcaggccgcg
      841 ctggatcggg cgttgaccga gcaggaaaaa ttgttctagc gcgtaagcag cctgcgcccg
      901 gttgtcccct gcggtgtttg tccgggctat attctgtgaa ccaattattc tatttggttg
      961 ctagcctgat gagcgctgtt gtgcgcgtcc gacgcggaag cgcctgatgt gctcggtaag
     1021 cgaccaccct tgaacctaag gttcaagaat gatgccttga cggcatttgc gggggacttt
     1081 aattcatgcg gcaatcagcc agcggctctg ccgcattttt tatgtgcggc ggccctcatg
     1141 cgttactggc tgatgaaatc cgagccttcc gaattcggca ttgacaaact cgcggctgct
     1201 cccggcaaaa ccacgccctg gttcggggtg cgcaactacc aagcgcgcaa cttcatgcgc
     1261 gaccaaatgc ggttgggcga cctggccttt ttctaccact cgagctgcaa agagccgggg
     1321 attaccggca ttgtcgaagt ctgcaagcct gcgtatccgg acgccaccca atacgaccgc
     1381 aagagctgct atcacgaccc caaggccacg ccgcagaacc cgcgctggtt caatgtggac
     1441 gtaaagctcg tcaacaaaac ccggctgatc cgcatcaagg agcttcgccg ccacccgcag
     1501 ctcaagacca tgcgcgtttt gcaaaaaggc aatcgcctct ccatcacccc ggtagatccg
     1561 agagagtggg agttcatcac tcgcgttctc ctgaagtgaa cgcgccgatt cacgctcgcc
     1621 ttaacgcaaa gggctgacga tggaatggtg ggcggcttat cttgcagtgg gtgctgtggc
     1681 gggttttctc gcaggattgc tgggagttgg cggcgggctt atcttcgttc cctcacttat
     1741 cgtcctcttc aacttgcagc attttccaca aactaacctg ctgcatcttg cgcttggcac
     1801 ctcgctggcg agtatcacgt tcacttcggt gtccagcctt cgcgcgcatc atgctcatgg
     1861 cgcggtcaac tggcagatcg tgcgccgcac aactcctggc atcgttgttg gcacgcttct
     1921 gggatcaggt ctcgctgcgc tgcttacgag ttccatctta gcgttgtttt tcgcggtgtt
     1981 cgtctctttg gccgccaccc agattttgct tgatatcaaa cccaagccct cgcgccagtt
     2041 gccgggcagg gccggcacat tctttgtggg aatcataatc ggcatccttt tcagcttggt
     2101 ggggggcggc ggcgcgatac tgtcggttcc atttatgctc tactgcaatg taaaaaccca
     2161 tgatgccatc ggcacctcgt cggcaatcgg ttttcccatt gccgtctccg gcgctgcggg
     2221 ctacattcta agtggaatgg cgcagaagag cctgccgccg ctgagcctgg gcttcgtgta
     2281 tctgccggct gtgctgtggc tggtgatagc aagcgtactc acggcaccgc tgggcgcgaa
     2341 gctcgcacat cgtttaccgg tggacaagct taaaaaaatc ttagccgttt tgctttatat
     2401 catcgccgcc aaaatgctgg tggggctact ttagattttc ttaggttttc ttggccgccg
     2461 ctttcgacag gcgatcacgc actgccgccc attcttcgcc tagcggcggt tcttcaacca
     2521 cgatcacatc acagcccgat tgatcgagct tgcgcaaatt ggcgtaaagt tcccgcgcgt
     2581 agtccgcggc tttcacgggc gccgcattcc aaaccatctg taaaccgcca tcaggcggcg
     2641 attgatatgc gagcaccgcc acgcgctggt tctggcggta cagactgcgt atgcattccg
     2701 ctaaattgcg cgtaacgagc agcggtgttt gcggcgcata gtgggaggca agcatgcccg
     2761 gtgcgcgcgt gccgcttgcc tcgggcttgc gcagcgttgt ccccaaaatc gcttcaattt
     2821 ccgccgcggt aattttcccc ggccgcagca acaccggatg ctcgctcgag acatcgagga
     2881 tggtggattc aatgcctacg gtgcaggatc cgccgtccag aatcccgtct atccgatcac
     2941 ccagctcggc acgcacatgc tccgccgtag tggggctcaa gcgaccgaag cggttggccg
     3001 agggcgcggc gatgccgccg ccgaacgttt gcagcaaagc ctgcgccacc ggatgagcgg
     3061 gaacgcgcaa acctaccgtg tcctgccccc ccgtcacggc atcagggacg cgcggcgcgc
     3121 ggcgcaaaat caaggtaagc ggcccaggcc aaaaacgccg ggcgagctcg cgagccgcac
     3181 ctggaatttc tccggcccag cgctcgaggt gagaactatc gggaagatgc acgatgaccg
     3241 gatgatcctg cggccggccc ttggcgatga agattttttt taccgcttcg ggattggaag
     3301 cgtccgctcc aagaccataa accgtttcgg tggggaaagc gacgaggccg cccgcgcgta
     3361 gaatggctac ggctttttca atctcattgt tgggcatttg atttaaccgc ggagacgcgg
     3421 agagaaactt taattcacag gattaacagg ttttttcaag acttacagga tttaatcttg
     3481 ttaatcccgc gaaatcctgt aaatcctgtc catttagttt cgcttgtctc tgcgcctcgg
     3541 cgcctctgcg gttgaataga tttaattgcc ggccagcaag cgcagcgctt cgtggtattt
     3601 ttccgcggtc ttggcaagca cgtcggcggg caaagccggg gcggggggct ttttgttcca
     3661 gcgctgcgcc tccagccagt cgcgcacata ttgcttatca aaactgggcg ggctcattcc
     3721 cgtccggtag tggcttgccg gccagaagcg cgaggagtcg ggcgtgagca cttcgtctat
     3781 cagatacaac ttcccttgct tgtcggtgcc gaactcgaac ttggtatcgg caatgatgat
     3841 gccttttttc gccgcatatt cggcggcttc agaataaagc tcgattgaag cgtcgcgtac
     3901 tctcgccgct atgtcctttc ccaaaagctt ctccgcttcg gcaaaagaga tgttttcgtc
     3961 atggctgccg gcaggcgcct tggtggaagg ggtaaaaatc ggctgcagca gtttttgcgc
     4021 ttccttcaag cccgcgggca agggaatgcc acaaatcgtt ccgcttttct gatattcctt
     4081 ccagccggag ccgacaatat agccgcgcac tatggcttcg atcggcaagg gcttcaagcg
     4141 ccgcaccacg aatgcgcgac ccgccacctg ttcacgctcg cgtgcttctt ttaccactgc
     4201 ttcaggcgca acgccggtga gatgattggg tatgacatgg ccaagttttt gaaaccagaa
     4261 attggaaagc gcggtcaaca ccttgccttt gccgggcacc ggcgtaggca aaaccacatc
     4321 gaaggcagag aggcgatcgg tctgcacgat cagcagtttg tcctcaccca cggcgtaaat
     4381 atcgcgcacc ttgccgcggt ggagaaaagg aatgctgcca agatcggact ggaatagaag
     4441 attatccgta cggttcattt attctttagg agttgcttca atcaaactag ggcaagcggg
     4501 cagtcttgat ggccttagcc tgtttcttgc ggaaatcctg gagctgtttg gcgagcttcg
     4561 tatcttcgac agcgagcatc gccaccgcaa acaacccggc gtttgccgcg cccgattccc
     4621 ctatggaaaa agtcgcaacc ggtacgccct tgggcatctg cacgatggag agcagcgaat
     4681 cgaggccttg cagatgtttg gacggcatcg gcacacccag caccgggagc gtggtcttgg
     4741 cggcaattac gcctgcaaga tgcgccgccc cgcctgcgcc ggcgatgaag cacttgacgc
     4801 ccgttttatc cgcgcttttg acgtattcat ccagcagacc gggggagcgg tgcgcggaaa
     4861 ttacccgcgc ttcgtaggaa acaccgaaat cttcgagctg ttttgccgcg tgctgcatca
     4921 cctcccagtc gctgcggctt cccatcagca cgctcaccag cgtttttttg gtcattattt
     4981 ctccgtgtcg tggctgctct gcaaactgtg aattttacac tatcgggccg ggcctcaact
     5041 cattttgccg cacccggctt cgcgctagct ttttcgtcgg gcacgccttc cttgaacacc
     5101 cggctcgcga gaatatcttc ggcttcaatc gtcaccaggt ctttcttcgt gagcttgccc
     5161 tgattgcgcg cgaacaggcg gttcgcctgg cgcaatctgg cgcggtcaag cgcattgcgc
     5221 acgctgcgcg cgttggcgaa atgcggcatc tgcctgcgct tgacgatgta atcctggaat
     5281 gccttatcgg cgtccgcgct gaagcggtat tgactcgctt ccagcatcag cctggcaatc
     5341 gccagcagtt cttccgccgt gtaatcggga aaatcgaggt ggtgggcgat gcgcgaggac
     5401 aaaccggggt tgctctggaa aaatttatcc atgcggtctt tgtaaccggc gaggaccacc
     5461 accagatcgt cacgctggtt ttccatcacc tgcagcagga tttctatcgt ctcctgtccg
     5521 tagtcgcgct cgttatccac gcggtagagg taatacgcct cgtcaatgaa cagcaccccg
     5581 cccatcgctt ttttcaggac ttccttggtc ttgggtgcgg tatggccgat gtactggccg
     5641 accaaatcgt cgcgcgtcac cgccaccaga tggcctttgc gcacgtaacc caggcgatgc
     5701 aggatttccg ccatgcgcag cgccacggtg gtcttccccg tgcccggatt gccggtgaag
     5761 ctcatgtgta agcttggcgc gcctgaagtt aaacccagct ccttgcgcag ccggtccacc
     5821 agcagcagcg ccgcgatttc gcggatgcgg gtcttgacgg gttttaagcc gatcagctcg
     5881 cggtcgagct ggtcgagcac ttccttgacg tgcgaatcgc gaaacgctgc ttcgaggtct
     5941 accggcttgt catccggcag attctgcggg atgccggggt ccgcactcat cagtcttggt
     6001 agcgctcgcc cttgggctgg ttagtcgcgt agctgcgaat agtatagtga atgcggcgcc
     6061 ccggtccttc ctgccgctcc agctggaatc ccggctcgtt cttgggccgg ttgacgatga
     6121 aagagagccg caccgtctcc cagccgtgcg tcgggtcaaa tgcattcacg cggatgtaat
     6181 gattgggata ggctttgcgg ccctggttga tctcgtacag gatgcctgcc gcgtccttca
     6241 ggtcaaacat cggcaagccc cacatttccc aataggtatt gcgcgggtgg ggatcatcgg
     6301 tgaattccac gctcaccgcc cagttgtttt tcaggcagta ttcgacttgc gcgcggattt
     6361 cctcgtcgga aaaatccggc aggaaggaaa aagtgccttg agtgattctc atgttcggtc
     6421 tccttaagcg ctcacgctgg gggtgggcac gaagtcggcg gtgtcggtgg aggcgtaatc
     6481 gaaggtgatg tccttccaca cttcgagcgc ggacttgagt ggggcgcaaa atttcgccgc
     6541 tttttccaga atccgcggcc cttcgttcca gatgtccttg ccttcgtttc tggcctgaat
     6601 tatcgcttcc agcgccacgc ggttcgcggt cgcaccggcc tggattccca tcggatggcc
     6661 gatggtgccg ccgccgaatt gcagcacgca gtcttcaccc aggtagtgaa tcaactggtg
     6721 catctgcccg gcgtggatgc cgccggaagc gaccggcatt actttgcgca agcccgccca
     6781 gtcctgctca aagaaaatcc cgcgctgcaa atccacttcg ttcctcggct cgcgcaggat
     6841 gttgtagtag ccctgcacca tgttggggtc gccctcgagt ttgcccacca ccgtgccggc
     6901 gtgaatgtgg tccactcccg ccaggcgcat ccatttggaa atcacgcgga aagacacgcc
     6961 gtggttcttc tggcgcgtgt aagtactgtg gccggcgcgg tgcaggtgca gaatcatgtc
     7021 gtttctgcgc gcccatttgg aaatcgactg gatcgcggtg taaccgatga tgaggtcaat
     7081 catcacgatg atcgaaccca gctccttggc gaattcggcg cgctcgtaca tgtcctccat
     7141 ggtcgcggcg gtgatattga ggtaatggcc cttgacttcg ccggtttcgg cgcaagcccg
     7201 gttcaccgct tccatgcagt agagaaagcg gtcgcgccag tgcataaacg gctgcgaatt
     7261 gatgttctcg tcgtctttca cgaaatcgag gccgcccttc agcgcctcat acaccacgcg
     7321 gccataattc ttgccggaga gccccagttt cggtttggtc gtcgcgccga gcagcggccg
     7381 accgaatttg tcgaggcgct cgcgctcgac cacgatgccg gtcggcggcc cgtcaaacgt
     7441 tttcacaaaa gccaccggca ggcgcatgtc ctcgagacgc agtgctttaa tcgccttgaa
     7501 gccgaacacg ttgccgatga tggaagcggt aagattggca atcgagcccg gctcgaacaa
     7561 atccagccca taggcgatgt aagcgaaata ctgtccgggg ttgttgggca ctggatccac
     7621 gcgataagcc ttggcgcggt acatctcgca agcggtgagc cggtcggtcc acaccaccgt
     7681 ccaggtggcg gtggaggatt cgcccgccac cgccgcggcc gcttcttccg gatccacgcc
     7741 ttcctgcggc gtgatgcgga acaaggttat gacgtccgtg tccttgggaa cgtagccggc
     7801 gtcccaatat cccattttct tgtagggaat gactcccgac ttgtagcgtt ccttaccctt
     7861 gggtgctttg tccggcccgc ccatgccgat ctccttcatc aactcgattc aaacacgctg
     7921 taatagtact ccaattaatc ataaatgata ctcacatgta ttgattgtta taataagatt
     7981 tttattatag tattgggctt gccgaattta tttaaagctc ccatgctgca tctaaccttg
     8041 agacaactgc gggtattcga agcggtggcg cggcacctga atttctcacg cgccgccgag
     8101 gaactgcatc tgtcgcagcc ggcggtttcc atgcagatca aacagctcga ggagaacgcg
     8161 ggactgccgc tgtttgagca gatgggcaag cgcatttatc tcaccgaagc cggacaagaa
     8221 atgttccagt acagccgctc ggttgcgcag cagctgcagg aaatggaagc ggtgctggat
     8281 cagatgaagg ggctggagcg cggcaagctc aatatatccg tggtcagcac cgctaattat
     8341 ttcgctccac agttgctcgc caaattctgc cagcaataca acaaggtcgc ggtcagcctc
     8401 agcgtttcca accgcgaagt ggtgcttaat caactcgccg acaatgaagc cgatctcgcc
     8461 atcatgggcc aaccaccgga aggcctggac atcgttgccg agtccttcat ggaaaatccg
     8521 ttggtggtga ttgcgccgcc caatcaccgc ttggcccggc agcgcaaaat tccacgcgcc
     8581 cggctgcagc aggaaacctt tttattgcgg gaaaccggct cgggcacgcg cagcgcgatg
     8641 gagcggttct tcgtcgaaca ccgcatcaag ctcaacaccg gcatggaaat gggcaccaac
     8701 gaggcgatca agcaggcggt gcaggcagga atgggtttgg ggataatttc acttcacacc
     8761 atcgaactgg agctggaaac gaaacggctt aaagtgcttg atgtagagag cttccccatc
     8821 aagcgtcact ggtatgtggt gcatcgccgc cagaaacggc tcaccaaagc ggcacaggcg
     8881 ttcaaggaat ttctgctcaa ggaagcgccg aaactgatgc gtaaaacgcg cgcatgagac
     8941 aaggcggtcg aagccgccta tgctttcgcg cttcagttgt ttaagccgcc gctttttccc
     9001 gctccatttg cgcggtatag cgcccatagc aggcggcgct gttcatcttc tcgcggtggt
     9061 aaagcgcctt ctgcgccgcc gccacattgc ccgcctggcc acgccaggtc ttgagcgccg
     9121 ggtcctgcag cgcgcgcccg taggagaagg agagcggcca ggagtgcgga cccatctgat
     9181 tcatgacatt gagatgggcg gtcgcgacct cggcgctttg gccgccggag aggaacacca
     9241 cgccaggaac cgcggcgggt accgcgcgct tcaggcagcg caccgtctgc tttgccactt
     9301 cttccacgct cgcctgcttc gggcatttgt ccccggaaat caccatgctg gctttcagta
     9361 tcatgtgttc cagggtcgcg cgctgttgat aaagcgcatt gaataccgtg cgcagcgtgg
     9421 cttcggtgac atcgaagcag cgctcgatgg tgtgattgcc ttccataatc acttccggct
     9481 cgacgatggg caccagcccc gcttcctggc acagcgcgga atagcgcgcc agcgcatgcg
     9541 cgttggcatc gagacagtat tggctgggaa tgccttcacc gatgttgatg accgcgcgcc
     9601 atttggcgaa gcttgcgccc aggccgcggt attccacgag ccgctcgcgc aggccgtcca
     9661 ggccttcggt cacttgttct cccggcgcac ccgccagttt cttggcgccg gtgtcgactt
     9721 taatgccgat caaaattccc tggcgcttca gcgcttcggg aaacggcgtg ccgtcggccg
     9781 tagcctgacg gatggtctcg tcgaagagaa tcatgccgct cacgtattgc ccgccgccgg
     9841 gaacggtaat caacaactcg cggtaggcac ggcggttttc cgtcgtggat tcaacgccga
     9901 tgctgtcgaa gcgcttcttg caggtagaac tgctttcgtc aatcgcaagg atgcccttgc
     9961 ctttggcgac catggcggcc gcggcgtttt caagttgctg tgcgtacata gccttacctc
    10021 ccgattaata tactggcgga gaacgtctgc agtgaaaaaa ccccccgcat accgccaata
    10081 aattgcgcgt ttcgcctgtg acgattttgg ctgcggccgc tgcgcttaac ttgactagcc
    10141 aagttagcct ccgccgaaat tgtcacttct ggcgctgttt gcctaagcga ttttggctgc
    10201 ggccgctgcg cttaacttga ctagccaagt tagcctccgc cgaaattgtc acttctggcg
    10261 ctgtgcacca actttaacaa ttttcattgt gttggtcccg cctgactcgc cgataggctc
    10321 gccgatagtg agcacaatca aatctccttc gcgcaccaca ccgttcgcca aaagcacttt
    10381 ttccgcttca cgcaacagct cctcgcgatc ctggcctaag tattgcacca ttagcggata
    10441 ggtatcgcga aacaggctta cgcggtatcg tgttgaggct tccgaagtca gcgcataaat
    10501 cggcacgccg caattcagcc gcgacatcca caatgcggtc gaaccggatt gagtgagcgc
    10561 ggcgattgcc ttcactttca aatggtaggc ggtaaacagc gccgccatgg ctatcgactg
    10621 gtcgacacgg gtgaaaacac gatcgagaaa ctcccgatcg agcgtgacct cggttgattt
    10681 ctccgcttcg aggcaaatgc ggctcattgc ttccactgtt tgcaccgggt atttgccgga
    10741 cgcggtttcc gccgacaaca tgactgcgtc ggtgccgtcg agcacggcat tcgccacatc
    10801 cgaaacctcg gcgcgggtgg gaatcgggct ctcgatcatg gactccatca tctgcgtcgc
    10861 ggtgatagca aacttgttca tctcgcgcgc catccggatc atgcgcttct gcagcgccgg
    10921 caccgcggcg tctcccactt cgaccgccag atcaccgcgc gccaccatga tgccatcgca
    10981 ggcctccatg atgtcctgga gcatgggtac cgcctcggcc cgttcgatct tggcaatcag
    11041 cagcgcctgg ctgttgatat tgcgcaacaa ctcgcgcgca atacgcatgt cagccccact
    11101 ttttggaaac gacaccgcga gataatccgc tttgagcatg actgcggtct tgatgtcttc
    11161 catgtccttg gcggtcaacg ccggtgcggt caggcccccg ccttggcggt tgatgccctt
    11221 gttgtttgac aacacgccgc cgtggcgcac cttgcaaatg atttcgttgc cgcgcaccgc
    11281 ctgcacctca agcacaatcc gcccgtcgtc aagcagcagc accgaaccct ccgcaacatc
    11341 gcgcggcaac tccctgtaat ccagcccgac gcgttcctgg ttgccgagcg cgccatctgc
    11401 gtcaagaatg aaggtgtcgc cggaattaag cgtaatcttg ccctgctcga attggccgat
    11461 gcggattttc gggccctgca ggtcagcgat gatgccgacc gtgcggccaa ccttgcgtgc
    11521 cgcctcgcgc accgtttcag cacgagaaag gtgatcctgc gccgtgccat gggagaagtt
    11581 catgcgcacc acatccacgc ctgcggtaat cattcgttcc atgatttttg gatcgttgga
    11641 ggcggggcct agggtggcga cgattttggt tctgcgcaac ataatcttat gcggagtaag
    11701 gggcataaaa gatgagttac tttagcaaat tcccggcacc ttattcatca cacctcactg
    11761 cttcgccgcc cgctgttcca gaatttccac tgcaggaagt ttcttgcctt caagaaattc
    11821 gagaaacgcg ccgccgccgg tggaaatata cgaaatctgg tcgattaccc cgtatttcgc
    11881 tatcgccgcg agcgtgtccc cgccggcagc aatggaaaaa gctttggatt ttgctatcgc
    11941 cagagccagg gctcgggtgc cttcgccgaa ctggtcaaat tcaaacacgc ccagcggccc
    12001 gttccacaaa atggtgccgg ctttttccaa aacgcttgcg agtttcgcgc tggttttggg
    12061 gccgatatcc atgattaaat catcgtccgc cacctcttcg gcatctttca aagtcgcatg
    12121 ggccgcctcg gaaaattctt ttgcgcacac cacgtccacg ggaatgggca catcgccgcc
    12181 cttggcgcgc gctgcgttga taatgccgcg cgcctcatca accattcctg catcggcaag
    12241 tgatttgcct atcggcaggc ccaccgcaag catgaaggta ttggcaatcc cgccgccgac
    12301 tatcaactga tccacgactt ggctcaaaga tttaagcgcc gtgagcttgg tcgaaacctt
    12361 ggagccgccg acgatggcaa cctgcggccg cttcgggttc tcgagcgccc ggcccagcgc
    12421 ctccagttcc gccgcgagca acggcccggc gcaggcgacc ttggcgaatt tagcaacccc
    12481 atgagttgaa gcttcagctc ggtgtgcggt gccaaaggcg tccatcacgt agacatcgca
    12541 aagcccggcc attcgcctcg ccagctcgtc gccgtttttc ttctcgccct tgttgaagcg
    12601 cacgttctcg aacagcacgg cttcgccggg agcaacttcg acgccgttca gccagttgtt
    12661 cagcaatttc acttcacgtt tcaacaattg cgaaaggcgg cgcgccaccg gtttcaggga
    12721 attggcttca tcgtacttgc cttcctcggg tcgcccgagg tgcgacatga gcattactct
    12781 ggcgcggttt ttcagggcat gctcaatcgt cgggattgac gcgcggatgc gcgtatcgtc
    12841 gaaaacctca ccattcttga gcggcacgtt gagatcttcg cggatcagca cacgtttccc
    12901 cgcgagatcg agatcggtca ttttcaaaac acgcatcgct gcttacctgg cattcacccg
    12961 ttgtttcatt tggcattcat ccaggcaacc gtcacatcca gcatgcggtt gctgaaaccc
    13021 cattcgttat cataccaaga caacactttg accatcgtgc cgccgctcac cttggtaagc
    13081 gtggcgtcga aaatcgagga ggccgggtcg tggttgaagt ccaccgacac cagcggcttg
    13141 tcgttgtagt tgaggaggcc tttcaattcg ccctgtgcgg cttccttcat caccctgttg
    13201 atttcctcgg tgctggttgg gcgcttggcg atgaagctca aatcgacgag cgaaacgttg
    13261 atcgtcggga tgcgcaccgc aaaaccgtcg agcttgccgt taagctccgg cagcaccagg
    13321 cccagcgccg ccgccgctcc ggtcttggtg gggatcatcg acatggtggc ggagcgggcg
    13381 cggcgcaggt cttcatgata gacgtcggtg agcacctgat cgttggtgta agcatgcact
    13441 gtggtcatga ggccgctgac gatgccgatt ttctcatgca gcaccttggc cagcagcgcc
    13501 aggcagttgg tggtgcatga ggcgttggaa atcacggtgt gtgaaggctt gagtatctta
    13561 tggttcacgc cgtacaccac ggtggcgtcc acatctttgc cggcgggcgc ggagataatg
    13621 actttttttg cgccagcctt gagatgggcg gaggcttttc ccttactggt gaaaagcccg
    13681 gtgcactcgt gcacgacatc gatattcaag tctttccacg gcagctccgt cggattgcgc
    13741 accgccagca cttttatgcg atcgccgttg acgaccatgc tgtcgccgtc cacttgcacg
    13801 gtgccgtgaa aacggccatg tgccgtgtca taacgggtaa gatgggcatt ggtctcggca
    13861 ttgcccagat cattgatggc gacgatctgg atctcctggt ggcgcccgga ttcataaagc
    13921 gcgcgcagaa tgttgcggcc gatgcgcccg taaccgttga tcgcgacacg aatggccatt
    13981 atttcgctcc ttcagggaaa aagccttatt ttaccgcagc gggcacctga aacgcagggg
    14041 gatgaagctt caaaccacgc ttttcacggc tttaaccaca gtctcgacag taaacccaaa
    14101 gtacttgaac aaatccgcgg cgggcgccga ttcaccgaag cggtctattc ccaccacagc
    14161 gccttccaac ccgacatact tgcgccagcc gtcggtgaca cccgcttcca ccgccacccg
    14221 caccacgccc ttgggcagca ctgaagactt gtaagcttca tcctggcggt cgaataccga
    14281 ggtgctgggc attgacacca cgcgcactgc aatgccttct tcggcgaggg ctttttgcgc
    14341 gcctacagcg agcctaactt ccgaaccggt ggcgataatt accgcgcgcg gttttcctcc
    14401 tggcgcttcc gcaagaacat aacccccctt cgcgatatgg gcaaaactga cggcgtcgcg
    14461 cttttggaaa ggcaggttct ggcgggtaaa caacaaggag gagggcccgt ttccgcgctc
    14521 aatcgcctgc gcccacgcca ccaccgattc caccgtatcg caggggcgcc acacgtccat
    14581 gttgggcata agccgcagcg tcgccgcctg ctccaccgcc tgatgggtcg ggccgtcttc
    14641 accaagcccg atggaatcgt gggtaaaaac aaaaatcgtg cggatcttca tcaatgccgc
    14701 cagacgcagc gcgttgcgcg cgtactcgga gaacatcagg aaggttccgc cataaggtat
    14761 gactccgccg tgcagggcga gcccgttcat gatcgccgcc atgccgaatt cgcgcacgcc
    14821 gtaataaata tagttgccgc catctttctc atcgacagcg cgcgaacccg accacaaggt
    14881 gagattcgag ccggtgagat cggccgagcc gccaatgagc tcggggagaa tcggcgccaa
    14941 gccttcgatg gcgttttgcg acgccttgcg ggtggcgatg ttctcggctt tttcgtttgc
    15001 tttggaaatc aattgcgcca catgctcgcg gaaatccgca ggcagttcgc cgttcatgcg
    15061 gcgggtgaat tccgcggctt ccctgggata ttgtttcgcg tattcggaaa acctgcgctc
    15121 ccattcgctg tccagcttgg cgccgcggcc gcgcgcatcc cagccttcat aaacatcctt
    15181 cgggatttca aacggcggat gattccagcc gatgttggcg cgggtggccg cgacttccgc
    15241 ttcgcccaaa gccgcgccgt gcgcatcgtg gctgcctgcc ttgttgggcg cgcccatggc
    15301 gatcaccgtc ttgcagcaga ccagcgtggg cttgtcggta acctgcttcg ctttcttgat
    15361 cgcggcatcc accgcatcaa agttgtggcc gtccacattg ggaaccacat gccaaccgta
    15421 agcttcgaag cgctttggtg tatcatcggt gaaccagccc tcgacatggc cgtcgattga
    15481 aatgccgttg tcatcgtaaa tggcaatgag cttgcccagg gccagttttc cggcgagcga
    15541 gcaggcttca tgcgaaatgc cttccatcag gcagccgtcg ccgaggaaaa cgtaggtgca
    15601 atgattgacg aggtcgaaac cgggacggtt aaaagtggaa gcgagtacct tctcggcaat
    15661 cgccatgccc actgcattgg ccagcccctg gccgagcgga ccggtggtgg tttcaatccc
    15721 cggcgcacag ccgcgttccg gatggccggg ggttttggaa tgcagttggc ggaagcgctt
    15781 cagctcctcc atcggcaagt cgtagccggt gagatgcaac aacgcatact gcagcattgc
    15841 gccgtggccg ttactgagga cgaaacggtc gcggtccggc catttgggat tggccggatt
    15901 gtgccgcagg tggtgattcc acagcacttc ggcgatttcc gccattccca tcggcatgcc
    15961 ggggtggcct gaattcgctt tctgcacggc gtccatagcc agcgcgcgaa tcgcgttggt
    16021 caaatccttg cgcgttgcca ttaacgggtt ccctgtattg atctcacccg ctgcagtagc
    16081 caaaaggagc ggggattcgg tagcaaaggt tatttaagga cagcaaaagc tggcgaatta
    16141 ttaccccgcc cgggagttcc agcaattaaa cattcttatc gcaattataa ctggaaccta
    16201 tcgtggggcg cgaagtttgc aaaaaaactt attgttcatg attgcgtcgg tcaagcttga
    16261 tgccgagctg cttgagcttg cggtacaggt gcgtgcgctc aaggcccact ttctccgcca
    16321 cccggctcat attgccgcgc tctttcatga tgtgatgctt gaagtaaacg cgctcaaacg
    16381 catcgcgcgc ttcgcgcaag gagacatcca gcggcagccc gtggtcggcg atttgcggtt
    16441 caaggtcgaa ctgcgccagc acgcggttcg cttcctcggc gctgatttcc tgttccagcg
    16501 cggtgagcgc gagcgtcttt accgcatttt ccagctgcag cagatttccg ggccagtcgt
    16561 aattgcgcag cgcgttcaag gccgaagtgc tgaattctcg cagcggcgtt tcatccaccc
    16621 ccatgcaatg cgagagcatg agccgcgcca gatcgggtat gtcttcacgg tgttcgcgca
    16681 ggctcgggac gcgcagagaa agcgccccga tgatttggta gagctcgacg tcaaaagcgc
    16741 cctggctgac gagtgtgggc agcatcctgg aggtggcgca aaccaagcgc acgttgtgtt
    16801 tcgctagttt gtcggctgcc agcaataagc ttttttgctc ctgcttggtg agctcgccga
    16861 cttcgcttaa gaacagcacg ccgtcgcgcg cccggcttaa cagatcctgg ggcgcttgcg
    16921 ccagttgcgc attgtcctgc ggcgccaccc aaggggtgtt gggctgatgc aggaaatggg
    16981 cgcacagttc catgccggct cccacctccc ctaccagcag cagcggctga ttcaaattgg
    17041 cgatctgctc caggcgcttt ttcaaatcgg tgacggctgg cgatttgccc aaactgttaa
    17101 gcgtaagcgg cggcaacacc tgggccgtgc cgcgcttcaa cgcccgtccc acggtgctca
    17161 acagcttttg catggcgatc ggtttttcca gaaaatcgaa ggcgcctatg cgggtggcct
    17221 ccaccgcggt ctcaatggtg ccgtggccgg acatcatcac caccggcatg gtgagcagcc
    17281 cgctgctcgc ccactccttg agcagagaga tgccgtcggt acccggcatc cagatgtcga
    17341 gcaagactag gtcaggccgc gcttccagac gctgggcgcg tgcctggctg gcattttccg
    17401 ccagccgcac ccggtagcct tcgtcttcta gaatctccga aagcagttcg cggatgccga
    17461 cttcgtcatc cacaatcaaa atctgattgg ttgccattcg tccatctcat caggccgcgt
    17521 gcgccagcgg cagcgagatg ctgacgcagg cgccgtgcgg tttaacgttt gcaatatgaa
    17581 tcataccgct gtgctcatcc acgattttct tgacgatggc gagtcccagg cccgtgcccc
    17641 tgggtttgct cgtcacatgc ggctcaaaca ggcgcgtcat caattgttcg gggaaaccgc
    17701 tgccgttgtc ctgaatactc aattgaatac ggccgttcac cgctgcagta cgcaccacga
    17761 tgccgggcgc cggcacctcg gtcagcgcat cttgcgcatt ctgcatcaga ttatgaatca
    17821 cctgccgcaa ttgggtcttg tctcccagaa cctggggcag atcaggtgcg agctccagct
    17881 tgatggcgag cgccggcgat tcataaagcg ccagcacttc gcgcagcagc cggttgaggt
    17941 cgagcgggta cagcgccagc tccggcgagc gggcatagtc gctgaacgcg tcgaccatgt
    18001 tctgcatcgc cgccacctgg ctgatgatgg tttgcgtggc acgcttgagg atttccaaat
    18061 cacgttgatc gagcttgttg gcgagcttgt actgcaaccg ttcggcagaa agctgaattg
    18121 gcgtaagcgg gtttttgatt tcatgcgcca agcgccgcgc cacctcgccc caggctgcgt
    18181 cacgctgcgc ttgcagcaaa tgagtgatgt cgtcgaacac caccacatag ccggcctgtg
    18241 cggcgccggc aaggcgggtg ccgcgcaata acagcacccg gatccccgat tcccctccat
    18301 attccacctg tttttcccac ccatcctggg gcgctcgctg gaatgattgc aatatttctt
    18361 ggctcaacac gcccaaagcc gcccggccgg cgcgccattc ctcgagacgg gttccgcgca
    18421 atcgcgcaaa ctcggtttcc agaatatatc ccgcgctggg attggccgaa cgcaaacgaa
    18481 atccttcgtc gaacgccagc acccccgctg acaggttggc cagcacgctc tccagataag
    18541 ccttggcggt ttgcagttgc tcttgatggc gttccgccgt catccgtgcc tccgccagct
    18601 ggtgggtcat gaggttgaac gactgtgtga gaacgccgag ctcatcgcgg cttttcaccg
    18661 gctgcctctg gctgaaatcg ccttgcgcca ccgcgcgcgt gccctcggca agcatttcga
    18721 gcggcgcgct catgcgctcg ctgatgagaa aacccagcgc gatggcagac agccacgcga
    18781 gcaacagagt gagcgtcaat gttatggcat acaggcgctt caaaccggca cgcgaaagcg
    18841 aaagctcttg ataatcgcgg taaaccattt gcaccgtttc cgcgtcttgc gccagctgcc
    18901 tgggcaccgg ctggagaagt tgcagcacgc ggatgccttc gttcacgttc aacacattga
    18961 ccggcaccat cacccgcagg tacagcccct tgcccggaat agtctcggcg ccgctatacg
    19021 cctgctgttg gcggatttgc ttaagcaagg ccgagctcgg caaatccggc atgagtccgc
    19081 ttttctcgct gatggaaaaa gcaatcaggg tgccgcgctg gctgaacagc gtggcttcct
    19141 gcacgcccaa ctgctcgcgc aaggtgttga gcgcggtcag gtgttcggcg actggcagct
    19201 cagagagcga gagcgccatc gtgtcggctt tcttgttaag gtccttgagc aggttatcca
    19261 acgtggtgcg cccaaggttc aagccgcctt ccagcgcttt ttcgacgcgc acatcgaacc
    19321 acgattcgat gctcttggcg agaaattgca ccgaaacggc gtacaccagc gcgccgggaa
    19381 gcaccgccat cagggagaaa aacagcacca ggcgcagggt gagcttcgcg ccgaacactt
    19441 tcgcggaaag cttcttgcgc aacgccagca actggaccgt caccacgccg ataaggcaga
    19501 gcgtgatggc accgttgagc gccagaatca gcgggtagtg ctgggcaaac agcgccgtgt
    19561 tggcgctggc ggtggaaagc aaaaacagca gaaccgcgcc cagaccgagg cacaggatca
    19621 cgaaatatct catgccgctt ctccgctcac ggggtgacct cccaacgatg ccagcccgag
    19681 cttaagttcc agtccttgga agcaactgcg tttaactgga atggtttggg cagttgcgac
    19741 acatcaagcc tcatgcgcag cgccgcgaca taggtcttgc ctttttccag cgcagctttt
    19801 tccaccgcca cccggtcgcg caactgactc atcagtccca tcgcctcctg cagagtagcg
    19861 aaattctgaa acaagttgcc cacactcaag cggtattgcc gggtcagcgc gttgtaactc
    19921 aacttgtact gatgtttgag ttgcaccagt ttctggtcga accagtacca gcgcgagcgc
    19981 gacaattcaa actcggtgac aaaatacagc gccacgccct tgttgagcgc ctcttccagc
    20041 gtatgggtaa gcgcgatatc caagtccgct tgcaggagat agtttttgcc gtccgccccg
    20101 aacgcggccg ctttcacctg tataccctcc gcaactaccg gcgccggcgc caggctcaac
    20161 agcatcacgc aggcgaatcc gcgtaaattg aacagcggcc aaaacctttt ccaaggcaga
    20221 ttaaatctta cggagcaagg cgtagaaaaa accatcatgc tggctgtcgg gtagcaattg
    20281 gccgtcggtg ttgagcggct gaagctcggc atttttgtgc cgcccaagaa acgcgtcaac
    20341 ttgctgacgg ttttcctctg caaagaccga gcaagtgaca tatagcaatt taccacctcc
    20401 gtccaggcat tgccacaagg catcgaggat gcgcgactgc tgtccggcaa actttccgac
    20461 gtcggtttcg cgccgcaacc acttgatgtc gggatgacgg cgcaccactc cagaagcgga
    20521 gcagggcacg tcggcaagga tgcgctggta aggcacgcca tcccaccacg tcgcgggctg
    20581 acctgcgtca ccgcacacaa tgcgcgcttt aagttttaag cgccgcaggt tttcttcgac
    20641 tcggcgcaat cgttcgccgt cgttatccag cgcggtaacc tcggcgtcag cctgctccag
    20701 aatatgcgcg gtcttgcccc ccggcgcagc acaggcatcg agcacacgca ttcctggccg
    20761 cacgtcgagc agcggcgcgg cgcgctgggc cgccgcgtcc tgcaccgaaa cctggccttc
    20821 ggcaaaaccg ggcagttctt ccaccatcag tggccgcttc agcatcaatg cgtccccgcc
    20881 cagatcgacg gcctcgattt gcgcgctttg cagccgcaaa tggtaatcgc gtttggtact
    20941 ggcgcgccgg ttcacccgca aagtcatcgg cggatgctgg ttggcagcca acaaaatggc
    21001 ttggtactgg tgtgggtatt gcgctttcag tttgtctatc caccattgcg gatgtgagta
    21061 gagaccgact tcattgtcgc gggcttgcct ggtcaaactt tcgcggtgac gaagaaaatt
    21121 gcgcaatacc gcattgacca ggccttttgc cgcccgctga ttgagcgttg ccgcggcgtt
    21181 gaccgcgtgg tcaaccaccg cgtagggcgc cgctttgccg tactgcaatt ggtagaggct
    21241 taccagaagc agcgcccgca gtttgtcatc gcgcaaagtt ttctccagca acaaaccgag
    21301 cagtgcctgc gcttccccgc aatagcgcaa ggtaccgtaa gcgagttcct gggcttgcgc
    21361 gcgctgttgc gcgcccaaag acggttggcg ccgccaaagc gcggagagcg tggaagtgag
    21421 attgtgcccc tccagcacct ggcttaccgc aaggctggcc aggcgctggg cttcaatcat
    21481 cgcgcgcggc tggcgagaat caggctctcg ccatggccaa gagacgggca atgcgctcct
    21541 gcgtcgaagg gtgggtgctg aacaatccct gcacgccgcc gccggagagg gggttgatga
    21601 tcatcatctg cgcggtttcc ggatgcattt cggcaacggg catagtctcg ccgcgggcga
    21661 aacgctcgat tttttccagg gcggaagcca gcgccccagg atcgcccgag atttccgcac
    21721 cgctgcggtc ggcttcgaat tcgcgcgccc gcgagatggc gagttgaatc agcattgccg
    21781 caatgggagc aacaatcatg atgagcaacg ccgccagcgg atgcacgccg cgcttattct
    21841 ggttgccgcc gccaaacaac ataccgaatt gcgcgacgga cgaaatagcg ccggcgatag
    21901 tggcggagag ggtggagatc aaaatatcgt gatgctggac gtgggcgagt tcatgcccca
    21961 acaccccgcg caactcgcgc ggggtgagca actgcaaaat gccggtggtc gcggccaccg
    22021 cggcatgctc ggggttgcgg ccggtggcaa atgcattggg ctgggcctga tcaatcaaat
    22081 acaccctggg catgggaaga tgcgcgcgtt gcgccagttc gcgcacgatg ccataaagct
    22141 caggggcgcg ggtttcatcc acttcctgag cgttgtacaa gcgcagcacc atcttgtcgg
    22201 agaaccagta ggcccagaga ttcattgcgc cgccaagaac caaagcaatc gccattcccg
    22261 agccgccgcc gagcagtgag ccgaccgtgc cgaaaagcgc cataatggcg gccatcaaca
    22321 aagaagtttt gagccagttt cccaacattg cggttccttt cacttagtct gccgcttggt
    22381 tcggcggcga ttcttttaga taagaccgaa accaaaaaaa ttcaagcgcc aagccgcgtg
    22441 cccggcttta aggcagaacc cgccaggaag gcttgcgccc gcaggcgctt gctacccgct
    22501 ttttgcagtt cttcgatacg caacccccct gtgccacaag ccacaacgat gccttggccg
    22561 ttcgcagcaa tcacctcgcc cgggttgcct gctacatgat tttcaacccc ggctttccag
    22621 attttgagcg gctcgctatt taaccgtgtg gaagccccgg gatggggatt aaacgcgcgt
    22681 accatgcgct cgattaagcg cgcttctttt ttccaatcga tttccgcttc ggttttctcc
    22741 accttggcgg cgtagcttgc gccagttcgc ggctgaagtt gcggctgcaa ttcgccgcgc
    22801 tcgagcgctt gaagcgctcg cacgatgcat tcccccccga gccgcgctaa tttatcatgc
    22861 aaagtctgcc cggtatcgtc gtcagtaatc tgcagacgct gttgcagcaa cagcggtccg
    22921 gtatccaggc c
//
LOCUS       DATIOW010000099         4032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_555568, whole genome shotgun sequence.
ACCESSION   DATIOW010000099 DATIOW010000000
VERSION     DATIOW010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555568"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="VLL03_03215"
     CDS             <1..944
                     /locus_tag="VLL03_03215"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HSS46406.1"
                     /translation="WPQDGPDVETLLQNADAAMYRAKELGRNNFQFYAAEMNTGASER
                     LSLQTMLRRALEREEFILYYQPKIELKNGRLSGIEALIRWNSSEIGLVQPFKFIPILE
                     ETGMIIDVGKWVMEKAVSDHARCLVEHPDFPRIAVNISQIQLHDKKFVETVKKTVEKS
                     ANAGAGLDFEITESLIMKDIEATIPKLRAIREMGIGIAVDDYGTGYSSLNYLVKLPID
                     ALKVDRTFIMNVDNRPENMTIVSSIISLAHSLNLRVIAEGVETLEQLKLLTLLKCEEV
                     QGYLICRPIPIDELLEWKARFSFEATKTDQRGSGAAT"
     gene            1379..1960
                     /locus_tag="VLL03_03220"
     CDS             1379..1960
                     /locus_tag="VLL03_03220"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HSS46407.1"
                     /translation="MKYCSNCGSPVRSLIPPGDTLPRYVCDVCTTIHYQNPKIVVGCI
                     PEWENKILLCRRAIEPRHGLWTLPAGFLENHETTIEGAVRETLEEAHARVEIGMLYAL
                     YNIPHINQVYLLFRGRLLDLNYQPGAESLEVRLFDERQIPWDELAFRTIRETLKAYFN
                     DRRNGAFSFHIGDILPPQASNELAQVAKLSHRI"
     gene            complement(2275..3393)
                     /locus_tag="VLL03_03225"
     CDS             complement(2275..3393)
                     /locus_tag="VLL03_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HSS46408.1"
                     /translation="MKNESVKPLASRRRVLMLLGGLLAAPKVFARLTTHESRLKPFAS
                     VVPGYRLRFPDDEGSHPGFRIEWWYITGWLNETTQPLGFQITFFRARPELKEENPSAF
                     TPHHILIAHAALSDTREGKLIHAQRAARGGFGLAGAEEERARVWIDDWLLTQENNAYR
                     VRLAAADFEFDFNFAVTQPPLLQGGIGLSRKGPAPESASYYYSLPHLQVIGTLVREGK
                     PQSVTGSAWLDHEWSSSYMAKEAVGWDWIGINLADGGALMAFRMRDKQGGSFWSGGTL
                     RRADGSVQSFSPSDIRFTPRREWRSARTGTSYPVSWSIKAGDLELAIEPLMDNQENDN
                     RATSGTIYWEGAVRALRQGKLAGLGYLELTGYWRRVDL"
     gene            complement(3411..>4032)
                     /locus_tag="VLL03_03230"
     CDS             complement(3411..>4032)
                     /locus_tag="VLL03_03230"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSS46409.1"
                     /translation="ARQTGAIIMDRDFYIQLTGDRLANDAALWLASDVPLQQAAMTLR
                     ERLADAPGIEIASTREVRASSLSVFDRTFAVTYALELAAILIGLFGVSVSFSAQALAR
                     RREFGVLRHVGMSRRDIGIMLGYEGMIVSVLGVGFGLACGWVVSLILIHVINRQSFHW
                     SMDLHVPWLPLAVLGIILIAAAALTAVWSGRSAMKEDVIRAVREDW"
BASE COUNT          918 a         1166 c         1124 g          824 t
ORIGIN      
        1 tctggcccca agatgggccg gatgtcgaga ctctgttgca gaatgccgac gccgctatgt
       61 accgcgcgaa ggagctggga cgcaataatt ttcagtttta tgccgcggaa atgaacaccg
      121 gcgccagcga gcggttgtct ctccaaacca tgcttcggcg tgcgttggaa cgggaagagt
      181 ttatcctgta ttaccagccc aaaatcgaac tcaagaatgg gcgtttgtcc ggaatagaag
      241 cactgattcg ctggaacagc tccgaaatag gactggtgca gccattcaag ttcattccca
      301 ttcttgaaga gaccgggatg attattgacg ttggcaagtg ggtcatggag aaagccgttt
      361 cagaccatgc gcgatgcttg gtagagcatc cggattttcc gcgcatcgcc gtgaatattt
      421 cacagattca actgcatgac aaaaagtttg tcgagacggt aaaaaaaact gtggaaaaat
      481 cggcgaacgc tggcgccggg ttggatttcg agataaccga gagcttgatc atgaaggaca
      541 ttgaggcaac tattcctaaa ctcagggcga tcagggagat gggcatcggg attgccgttg
      601 acgattatgg cactggttat tcttcgctga actatttggt aaagctgccc atcgatgcgt
      661 tgaaagtgga tcgaacgttc ataatgaatg tcgacaaccg ccccgagaat atgacgattg
      721 tttcgtccat catttccctc gctcattcat tgaacctgag ggtgatcgcc gagggcgttg
      781 aaaccctcga gcagttgaag ctgctgacct tgctgaaatg cgaagaggtc cagggctatc
      841 tcatctgtcg gccaataccg atagacgaac tgctggaatg gaaagcgcgg ttcagctttg
      901 aggcaacaaa gacagaccaa aggggttctg gtgcggcaac ttaacgaccg gtggttcgaa
      961 caagtgtttt gattttgttc tgtaccgaca tgggttagtc aagaacgcaa aaacgggtca
     1021 aactaacctg caattcaaca gctcgtagac cggaggctcc attgagtcac tcccagatgc
     1081 tgaagactag gcgaaggaag caaaaagcca ggaagcacct cgcgggagca gccaagcgag
     1141 caaagaagct gaggaagcaa gacgtgaaga gggtcagtgc ggacgctctg aaaaaaggat
     1201 ccccctagcc gcttttcaac gcgaaccggg gaacccaatg cgcccgccgc gtgcacccgc
     1261 ttaacggctg ctttggggcg atagcagcaa ttcaacctga aacactaccc cattttctcc
     1321 ctcgtttcgg ggagcacggc tgcttgcggt atataattaa gttcaatctg gaacgaacat
     1381 gaaatactgc agcaactgcg ggtcacccgt gcgctcgctc attcctcccg gcgacacctt
     1441 gccgcgttac gtctgtgatg tctgcaccac cattcattac cagaatccca aaattgtggt
     1501 gggctgtatc cccgaatggg aaaacaaaat cctcctgtgc cggcgcgcca tcgagccgcg
     1561 ccatggcctg tggacgctgc ccgcgggctt tctggaaaat cacgagacca ccatcgaggg
     1621 cgcggtgcgc gagacgttgg aagaagctca cgcgcgggtg gaaatcggca tgctgtacgc
     1681 gctttataat attccgcaca tcaaccaggt gtatcttctg tttcgcggcc ggttgctcga
     1741 tttgaattac cagcccggcg ccgaaagcct cgaggtgcgg ttattcgacg agcggcaaat
     1801 cccgtgggat gagctcgcgt tccgcaccat tcgcgaaacg ctgaaagctt atttcaacga
     1861 ccggcgcaac ggcgctttca gttttcacat cggcgacatc ctgccgcctc aggcaagcaa
     1921 cgagcttgcc caagtcgcca agctttcgca tcgcatatga aaagcgccgt gcccgaagaa
     1981 gatttggcca ggctcaaaat cagccgcgac agcacagcaa tcagccccat tccggcaaaa
     2041 aacaaaatca ggcgagcgct gctcgtcgcg ctgccgttcc tcattgcggc aggccgccga
     2101 gttcgaacga cgcgccgctg tctcttaaat cactgtccat gaaacccggt acagctcaac
     2161 ctgacccctt tccttctata tcaatggcga gacaactagc aataagagca tcaattcacc
     2221 cataatcatc ctccctcaat tctatagatt agatatgggg tcagctcact ccacctaaag
     2281 atcgactcgt ctccaataac cggtgagttc cagatagccc aaacctgcaa gcttgccttg
     2341 gcgcaatgcg cgcaccgcac cttcccaata gattgttccg ctggtcgcgc ggttatcgtt
     2401 ctcctgattg tccattagtg gctcgatagc taattcgaga tcgcctgcct ttatcgacca
     2461 tgataccggg tagctcgttc cggtacgcgc ggaacgccac tcgcggcgcg gggtgaagcg
     2521 aatatcagaa ggcgaaaagc tttgcaccga gccgtcggcg cggcgcaaag tgccgccgga
     2581 ccagaagctg ccgccttgct tgtcgcgcat gcggaaggcc atcagcgcgc caccgtcagc
     2641 gaggttgatg ccaatccaat cccagccgac cgcttctttg gccatgtagc tcgatgacca
     2701 ctcgtggtcg agccaagcac tgccggtaac ggattgcggc ttgccctcgc gcacgagcgt
     2761 tccaatcacc tggagatggg gcaggctgta gtagtagctt gcggattccg gcgcgggacc
     2821 tttgcggctc aagccgatcc cgccctggag caagggcggc tgggtgacgg caaaattaaa
     2881 gtcgaactcg aaatccgctg ccgccagccg cacgcgatac gcattgtttt cttgagtaag
     2941 taaccagtcg tctatccaga cgcgggcgcg ctcttcttcc gcgccggcga gaccgaaacc
     3001 gccgcgcgcc gcgcgctgcg cgtgaatcag cttcccctcg cgggtgtcgc ttaaggccgc
     3061 gtgcgcaatc aggatgtgat gcggcgtgaa ggcgctcgga ttttcttcct tgagctccgg
     3121 ccgggcgcgg aagaaagtga tttgaaaacc gagcggctgc gttgtctcgt tgagccagcc
     3181 cgtaatgtac caccactcga tacggaaacc cggatggctc ccttcatcgt ccggaaaacg
     3241 caatcggtaa cccggcacca cggaggcgaa gggcttgagt cgtgattcgt gagtcgtgag
     3301 tcgtgcaaaa acttttggcg cggcgagcag tccgcccagc aacatcagca cgcgccttcg
     3361 acttgcaagt ggttttacgc tttcattctt catccttcta ccttcatggt ttaccagtcc
     3421 tctcgcaccg cgcggataac gtcctccttc atggccgagc ggccgctcca caccgcggtt
     3481 agggccgcag ccgcgatcag gatgatcccc agcacggcaa gcggcagcca cggcacatgc
     3541 aggtccatgc tccagtggaa tgactggcgg ttgatgacgt ggatgagtat caggctcacc
     3601 acccagccgc aagcgagccc gaaccccact cccaggaccg atacaatcat cccttcgtaa
     3661 cccagcatga tcccgatgtc gcggcgtgac atgccgacgt gacgcagcac gccgaattcg
     3721 cgccggcgtg cgagcgcctg tgcgctgaag ctcacgctta cgccgaacag gccgatcaga
     3781 atcgccgcca gctccagcgc gtaggtcacc gcgaaggtgc ggtcaaagac ggaaagcgat
     3841 gacgcccgca cctcgcgggt actcgcgatc tcgatgccgg gtgcgtccgc aagacgctcg
     3901 cggagcgtca tcgccgcctg ttgcagcggt acgtcgctcg cgagccacag tgcggcatcg
     3961 ttggcgaggc ggtcgccggt gagttgaatg taaaaatcgc ggtccatgat gattgcgcct
     4021 gtttgccgcg cg
//
LOCUS       DATIOW010000100         6719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1877154, whole genome shotgun sequence.
ACCESSION   DATIOW010000100 DATIOW010000000
VERSION     DATIOW010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6719
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1877154"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..247)
                     /locus_tag="VLL03_03235"
     CDS             complement(26..247)
                     /locus_tag="VLL03_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46410.1"
                     /translation="MGQLPQRAPKPRFINLTCKNPWIAIGGMAHRSAAKTAMGETLRY
                     RLEVNYIPRVRKVLIKELNRSSALFSVVG"
     gene            549..1001
                     /locus_tag="VLL03_03240"
     CDS             549..1001
                     /locus_tag="VLL03_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HSS46411.1"
                     /translation="MDLILWRHSEAEKGMPDLKRKLTKRGLKQAEAVAEWLIPRLAER
                     YKIYVSPADRARQTAEALGLPFTLSEQVSPTATAANILAQVNWPSAKGAVVVVGHQPA
                     LGQVAALLLSGNEANWSIKKGALWWFSNRLRDDETQTVLRAVITPDML"
     gene            1228..3375
                     /gene="ppk1"
                     /locus_tag="VLL03_03245"
     CDS             1228..3375
                     /gene="ppk1"
                     /locus_tag="VLL03_03245"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003918.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HSS46412.1"
                     /translation="MDLETRQVTMLPQLIPLKPSFVTTDSMAKNFKASHYLNRELGAL
                     EFNRRVLAQAENPEAPLLERLKFLCIFSSNMDEFFEIRVAGLKEQIKLNALGAGPDGI
                     KADEVFRLVSQQAHAMVARQYDLFNNAVVPGLAERSIQFLRRSNWTKAQQEWIRDYFF
                     REMIPVLTPIGLDPAHPFPRLFNKSLNFMVELEGRDAFGRNSGTAIVQAPRVLPRVIQ
                     LPRELTSCEYSLVFLSSILHKHVGELFAGMNVLGCYQFRVTRNSDLFVDEEEIKNLRV
                     ALQGEFPQRHFGDAVRLEVAETCSPQMVEFLLQQFGLGEEDLYRVNGPVNLVRLMQIP
                     EQVDRPDLKYPVFLPGVIKTLEKRQDIFKNIRKNDILLHHPFQSFNPVISFVQQAATD
                     PKVVAIKQTIYRTGMDSVLMEALINAARSGKEVTVVLELKARFDEEANLNWAGRLEEV
                     GAHVVYGVVGHKTHAKIAMVVRREADKLRRYVHLGTGNYHTSTAKLYTDFGLFTCNEE
                     ICEDVNEVFKQLTGLGKAGKLNHLWQSPFNLHQKIVEAIRNEAKQAEAGKPAAIIAKM
                     NALLEPEIIEALYEASKAGVKTDLIIRAVCALRPGLPGLSENIRVRSIVGRFLEHSRI
                     FYFQNGGNEDIYLSSADWMDRNFFRRIELCFPVLDPRLKKRVINEGLKPYLADNRQTW
                     EMDADGQYHQKTSESGKIRCAQEELLSMLASAT"
     gene            3490..4257
                     /locus_tag="VLL03_03250"
     CDS             3490..4257
                     /locus_tag="VLL03_03250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HSS46413.1"
                     /translation="MRTRAVLLSQTQTRQTARKSLAVSFARRESEVREAQRLRYKIFA
                     EEMGAKLTGRERGLDQDMFDAHCEHLLVRDTQSNEVVGTYRILNSTQAKKTGGFYSDD
                     EFDLTRLDPLRNGMVEVGRSCVHPDYRSGRTIALLWRGLTTYMERYRYQYLVGCASIT
                     MSDGGHVAASVYNKIKQGSLSPNEYRVFPHCGLPLDALNGNLPAPIPPLLKGYLRLGA
                     YVCGAPAWDSDFNTADLLILLPMYRINPLYAKHLFRK"
     gene            4394..5434
                     /locus_tag="VLL03_03255"
     CDS             4394..5434
                     /locus_tag="VLL03_03255"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSS46414.1"
                     /translation="MQLMVLRLAAFFLLASAGLACAASGRQVTDATGREIALPAEVKR
                     VYAAGPPASVFAIAPEKLIGWTQAMRSDEAAFLPPQYADLPELGRLTGRDNTANVEVV
                     LAAKPDLIVDAGSTAPTFVSLAQKVETQTGIPYLLFDGRLSETAKSFRALGEAVGEKA
                     RGDALADYVERMLAEIRDRIAKVSTTKRPRVYYAHGANGLTTALEGSINVEALELLNL
                     ENVAGKSEISSGLTNVSFEQVLAWNSDIIVTTDEVFYHYTVRLDPRWASLTAVRAKRV
                     YLSPHLSYGWFDFPPGPNRLIGAQWLAKILYPEIFTEDLKPKVKTFYRLFYHHESGDS
                     EVEALLSPENAK"
     gene            5455..6456
                     /locus_tag="VLL03_03260"
     CDS             5455..6456
                     /locus_tag="VLL03_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HSS46415.1"
                     /translation="MAWLYAGASGLTLVLVLLAFWLGKFPVSTGDVLAALAGKLFGVS
                     VTLPVNADAVIWQIRAPRIAAALLVGAGLAAAGSSYQGMFKNPLVSPDILGVSSGAAL
                     GAVLGIFFSKNIVMIQFFAFMGGLAAVGLVYIISSSVRGHDALLTLILTGVVIGTLLG
                     SFIALLKYLADPYNQLPAITFWLMGSLAAIAPSDLAVAAPLIALGLVPLYLLRWRINL
                     LSLSDDEARALGVNVVGMRLIVIVAATLMTAASVAISGIIGWIGLLMPHIARMLVGPD
                     FAKAMPMSLLLGAAFMLGVDTLARTAANIEIPPGVLTALIGTPFFVWILATARRGWH"
     gene            6460..>6719
                     /locus_tag="VLL03_03265"
     CDS             6460..>6719
                     /locus_tag="VLL03_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSS46416.1"
                     /translation="MQLIAENLTYGYPQRVIGIGLSFSLSAGEALCVLGPNGSGKTTL
                     FRTLLGLLRAKEGQILLDGRELAEWPRTELAQALAYVPQAHEG"
BASE COUNT         1471 a         1903 c         1861 g         1484 t
ORIGIN      
        1 acacccgctc gatttttcca cctagtcatc caacaacaga aaacaatgcg ctcgagcggt
       61 tcagttcctt gatcaaaacc ttgcgcaccc ttggaatata gtttacttcc agccgataac
      121 gtagcgtttc acccatcgcc gtctttgcag cactcctatg cgccatcccg ccgatggcta
      181 tccatgggtt cttgcaagtt aaattgatga agcggggctt cggtgcccgt tgcggtaatt
      241 gacccatcga ccgcgtccca ttaatggaac gaagtggtaa tctttcataa gggcttcaat
      301 ttcgctgccg gtcaacatgg ttggcttcat cacagaaagc gcggaatagc ttcccccgcc
      361 ttaagattat ttaccggcaa cattaattat tatgaagctt tgattacagt tagcggctca
      421 cccccttgtg ccaggaggtt tgcgtaatca acaatttctt tataaaactt taaagaaaca
      481 gcgctatatt tatagattta attgaacgga aagaagcaag ttaatccttc taaaaaaggg
      541 agcaaagcat ggacttgatc ctgtggcggc actcggaagc cgagaaagga atgccggacc
      601 tcaagagaaa gctcaccaag cgcggattga aacaagccga agcggtcgcc gaatggctta
      661 tccccagact cgccgaaagg tacaaaattt atgtcagtcc cgccgaccgg gcaaggcaaa
      721 cagcggaagc gctgggactg ccttttaccc tgtccgaaca agtcagtccc acagccacag
      781 cagcgaatat actggcgcaa gtgaactggc ccagcgcaaa aggcgcagtg gtcgtggtag
      841 gtcatcagcc agcattgggg caggtggcgg cgctgctgct ctccggcaac gaagcaaatt
      901 ggagcatcaa gaagggggcg ctgtggtggt tcagcaatcg gctgcgtgac gatgaaacac
      961 aaaccgtgct gcgcgcggta atcacaccgg atatgctgta aaacaaaaca gctcgtctgc
     1021 ccggcaggca atttgccttc actctgttaa gcctcatgcc gtgcgcaaga accttatggt
     1081 ctttgatggc cgctgcgatg ggcgacgctg tcaagccatt atggtatttt gttaaatctt
     1141 tgttaaatct atggaatcct tgactgtaac atggctgtca caattccacg gtattcttgg
     1201 tgatactatt gaaggtaagt ttatgcaatg gatctggaaa cacggcaggt aacaatgctt
     1261 ccgcagctca tccccctcaa accgagtttc gtcaccacgg attccatggc taagaatttc
     1321 aaagcctcgc attatctgaa ccgcgagctg ggcgcgctgg aattcaaccg gcgcgtcctg
     1381 gcccaggcgg aaaacccaga ggcgccgctg ctcgaacgtc tgaaatttct ctgcatcttc
     1441 agcagcaaca tggacgagtt ttttgaaatc cgcgtagccg gcttgaagga acagatcaag
     1501 ctcaacgctt tgggcgccgg ccctgacggc ataaaagccg acgaagtgtt ccgcctggta
     1561 agccagcagg cgcacgccat ggtcgcgcgg caatatgatc tgttcaacaa cgccgtcgtg
     1621 ccgggcctcg ccgagcggag catccagttt ttgcggcgtt ccaactggac caaggcgcag
     1681 caggagtgga ttcgggatta tttctttcgc gaaatgatac cggtgctcac cccgatcggc
     1741 ctcgatccgg ctcatccttt cccgcgcctt ttcaacaaaa gcctcaactt catggtggaa
     1801 ctggaagggc gcgacgcctt cgggcgcaat tcaggcacgg ccatcgtgca ggcgccgcgg
     1861 gtactgccgc gggtgatcca attgccgcgg gagctcacca gctgcgaata cagtctggtg
     1921 ttcctctcct ccattctgca caagcatgtt ggagagctgt tcgccggaat gaacgtgctc
     1981 ggttgctacc agttccgagt cacgcgcaac agcgacctgt tcgtggacga ggaagaaatc
     2041 aagaacctgc gcgtggcgct gcaaggggaa ttcccgcagc gtcatttcgg cgacgcggtg
     2101 cgcctcgaag tagctgaaac ctgctcgccg caaatggtgg aattcctgct gcagcagttc
     2161 gggctcggcg aagaagatct ttaccgcgtt aacgggccgg tcaatttggt gcggctcatg
     2221 caaatacccg agcaggttga ccgtcccgat ctcaaatatc cggttttcct gcccggcgtc
     2281 atcaagacgt tggaaaagcg ccaggatatt ttcaagaaca tccgcaagaa cgacatcctg
     2341 ctgcaccacc cttttcaatc cttcaaccca gtcatcagct tcgtgcaaca agcggcgacc
     2401 gatcccaaag tagtggcgat caagcagacc atttaccgca ctggaatgga ttcggttttg
     2461 atggaagccc taatcaacgc cgcgcgcagc ggcaaggaag tgacagtggt tttggagctg
     2521 aaagctcgtt ttgacgagga agccaatctg aactgggcgg gccggctcga agaagtgggg
     2581 gcacacgtgg tgtatggggt ggtgggccac aaaacccacg ctaagatcgc catggtggtg
     2641 cggcgcgagg ctgacaagct gcgtcgctat gttcatctcg gcaccggcaa ttaccatacc
     2701 tccaccgcca agctctacac cgacttcggc ctctttacct gcaacgagga gatttgcgag
     2761 gacgtgaacg aagtattcaa gcagctcacc gggcttggca aggccggcaa gctcaaccac
     2821 ctgtggcagt cgccattcaa tctgcatcag aaaatagtcg aggccatacg caacgaagcg
     2881 aagcaggcgg aagccgggaa acccgctgcc attatcgcca aaatgaacgc gctgctggag
     2941 ccggaaatca tcgaagcgct ttacgaggca tcgaaagcgg gagtgaaaac agatttgatc
     3001 attcgcgccg tttgcgcctt gcgcccaggg ctgccgggct tgtcggaaaa catccgggta
     3061 agatcaatag tcgggcgctt tctcgaacat agccgtattt tttatttcca gaacggcggc
     3121 aatgaggata tttacctttc cagcgccgat tggatggacc gcaatttctt ccggcgcatc
     3181 gagctctgct ttcccgtgct cgacccgcgt ctgaaaaagc gcgttatcaa cgaaggtctc
     3241 aaaccctatc tcgccgataa tcgccagacc tgggaaatgg atgcagacgg ccaataccat
     3301 caaaaaacgt ccgaatccgg taaaatccgc tgcgcccagg aagaactgtt gagtatgctc
     3361 gcctccgcaa cctgaattat gtcttgccgc ctgttacgcg gctggttttc aggacattgt
     3421 caagaaatca tcatctgcgc gtcatagccg tgtcatggct tacagttagt gtgccgctgt
     3481 cgcatatcca tgaggacgcg agccgtgctg ttaagtcaaa cccaaacccg tcaaaccgca
     3541 cgaaaatccc ttgctgtttc attcgcgcgc cgcgagtcag aggtacgcga ggcgcagcgg
     3601 cttcgctaca agatattcgc cgaggaaatg ggggcgaagc tgaccggccg cgagcgcggg
     3661 ctggaccaag acatgtttga tgcccattgc gagcacctgc tggtgcgcga cacacaaagc
     3721 aacgaagtgg tgggaacgta ccgcattctc aacagcacgc aggcaaaaaa aaccgggggg
     3781 ttttattccg acgatgaatt cgatcttacc cggctcgacc cgctgcgcaa tggcatggtc
     3841 gaggtgggcc gttcctgcgt ccatccggat tatcgcagcg gcagaaccat tgctctgctg
     3901 tggcgcgggc tgaccacgta catggagcgg taccgctatc aataccttgt cggctgtgcc
     3961 agcattacca tgagcgacgg cggccatgtc gccgccagcg tttacaacaa aatcaaacag
     4021 ggcagtctaa gcccaaatga atatcgtgtt ttccctcatt gcgggctgcc cctggatgcc
     4081 ctcaatggta atcttccagc gcccataccg ccgttgctca aaggttacct gcggctcggc
     4141 gcctacgtgt gcggcgcacc ggcttgggat tcggatttta ataccgccga tttgctcata
     4201 ctcctgccga tgtacagaat caatccactt tacgccaaac acttgttcag aaagtaagcc
     4261 tcacaagctc tcagtcccga tagcgtccat aagcctgtct gccgacgtct tttaatggtg
     4321 aaagcgccat gatacagttt gctgcgctct gagcgaagaa cattcgggtt aattcagagg
     4381 tttttcatat tccatgcaat tgatggtatt gcggcttgcc gctttttttc tccttgcctc
     4441 agccggcttg gcttgcgcag ccagcgggcg gcaggtgacg gacgcgaccg ggcgcgagat
     4501 agcgctgccg gcggaagtga aaagggttta tgccgccggc ccgcccgctt cggtgtttgc
     4561 gatcgcaccg gaaaagctca tcggctggac gcaggcgatg cgttccgatg aagcagcgtt
     4621 cctgccgccg caatatgcgg atttgccgga actcggccgg cttaccggcc gcgacaacac
     4681 cgccaacgtg gaagtggtgc ttgcggcgaa acccgacctg attgttgacg ccggctccac
     4741 cgcgccgact ttcgtctcat tggcgcagaa agtggaaacg cagaccggca taccctatct
     4801 cttgttcgac ggcagactct cggaaacggc aaaatcattt cgcgcgctgg gcgaagccgt
     4861 cggcgaaaaa gcgcgaggcg atgcgctcgc cgattatgtg gaacggatgc ttgccgaaat
     4921 tcgcgacagg atcgcgaaag tgtcaacaac caagcggccg cgggtttact atgcacacgg
     4981 cgccaatggt ttgaccactg cgctcgaagg ctcgattaac gtggaagcgc tggaactcct
     5041 caacttggaa aatgtcgccg ggaaaagcga gatttcgagc ggccttacca atgtttcctt
     5101 cgagcaagtg ctggcatgga actccgacat tatcgtcacg actgacgaag tgttttacca
     5161 ctacaccgtg cggctggatc cgcgctgggc aagcctgacg gcggtgcgcg ccaagcgcgt
     5221 ttatctttcg ccgcatctgt cttacggctg gtttgatttt ccgcccggac ccaaccgttt
     5281 aatcggcgcg cagtggctgg ccaaaattct ctatccggaa attttcacgg aagacctcaa
     5341 gccaaaagtg aagactttct accgcctgtt ttatcaccac gaatccggtg acagcgaagt
     5401 tgaagcactg ttgagtcctg agaacgcgaa atgatcccgt ccacatcacg cggcttggct
     5461 tggctttatg ccggcgcatc aggcctgacg ctggttctgg tgttgctcgc tttctggctc
     5521 ggcaaatttc cggtttcgac cggcgatgta ctcgccgcgc ttgcgggaaa actgtttggc
     5581 gtttccgtca ctctgcccgt caacgccgac gcggtaatct ggcaaatccg cgcgccgcgc
     5641 atcgccgctg cattgctcgt gggggcgggg cttgcagccg ccggttcctc ttaccagggc
     5701 atgtttaaaa atccgctggt ctcgcccgac attctcggcg tgtcgtccgg cgcggcgctc
     5761 ggcgcagtgc ttggcatctt cttttcaaaa aacatcgtca tgattcagtt ttttgcgttt
     5821 atgggcgggt tggccgcagt ggggctggtc tatattatca gttcctcggt gcgcggccat
     5881 gacgcgctgc ttacgctgat tttgaccggc gtggtgatcg gcacgctgct gggcagcttt
     5941 atcgcgctgc tcaagtatct cgccgacccc tacaatcaat tgcccgccat cacgttctgg
     6001 ctgatgggca gcctcgccgc aattgcgcct tccgatctcg cggtggccgc gccgttgatt
     6061 gcgctgggat tggtgccgct gtatctcctg cgctggcgca tcaacctgct ttcgctatcc
     6121 gacgatgaag cacgggcgct cggcgtcaat gtcgtcggca tgcgcctcat cgtgattgtc
     6181 gcggccacgc tgatgactgc ggccagtgtt gccatttccg gaatcatcgg ctggattgga
     6241 ctgctcatgc cgcatatcgc gcgcatgctg gtcgggcccg attttgccaa ggccatgccg
     6301 atgtcgttgt tgttgggcgc ggcgttcatg ctgggcgtgg atacactcgc gcgtaccgca
     6361 gcgaatatcg aaattcctcc cggcgtgctg acggcgttga tcggcacgcc gttttttgta
     6421 tggatattgg caaccgcgcg ccggggttgg cattaacaca tgcagctcat cgccgaaaat
     6481 ctgacttacg gctacccgca gcgcgtcatc ggcatagggc tgagtttttc gcttagcgcc
     6541 ggtgaagcgc tgtgcgtgct cggccccaac ggtagcggca aaaccacgct atttcgcaca
     6601 ctgctgggcc tgttgcgggc gaaagaaggc cagattctgc tcgacggcag ggaactcgct
     6661 gaatggccac gcaccgaact cgcgcaagca ctggcctacg taccacaggc tcacgaggg
//
LOCUS       DATIOW010000101         6637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_150919, whole genome shotgun sequence.
ACCESSION   DATIOW010000101 DATIOW010000000
VERSION     DATIOW010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150919"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VLL03_03270"
     CDS             complement(<1..388)
                     /locus_tag="VLL03_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004674289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HSS46417.1"
                     /translation="MVNRYSGVIERYRGRLPVSAATPVVSLCEGGTRLIELKNLPRLI
                     KKNVRLYAKFEGLNPSGSFKDRGMTLAVTKAVEAGAKAIICASTGNTSAAAAAYAARA
                     GIACFVVIPEGKIALSKLSQAMMHGSL"
     gene            complement(605..1921)
                     /locus_tag="VLL03_03275"
     CDS             complement(605..1921)
                     /locus_tag="VLL03_03275"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HSS46418.1"
                     /translation="MKPINVGLLGIGTVGGGTFTVLRRNQKEITRRAGRGIVIRMVAD
                     KDVAKARKIAGKNAVVTGDAMEVIRHPEIDIVVELIGGATAAKQFILKAIENGKHVVT
                     ANKALLAKHGNEIFAAAQKHGVMVAFEAAVAGGIPIIKALREGLTANRVEWIAGIING
                     TSNYILSQMREKGVSFDAALKQAQALGYAEADPTFDIEGIDSAHKLTILAAISFGIPM
                     QFDKVYTEGISKLTREDIRYAEELGYRIKLLGITKRTPKGIELRVHPTLIPARRLIAN
                     VEGVMNAVLVKGDAVGATLYYGAGAGAEPTASAVVADLVDVTRMHTAYRKHRVPHLAF
                     QPDALTDTPILPMSEVQTSYYLRLRVFDKPGVLADITRILADSRISIDAMVQKEPSEG
                     EEQVDIIMLTHETVEKRINAAIRKIEKLSTISGKVTRIRLEELDRN"
     gene            complement(2012..3196)
                     /gene="alaC"
                     /locus_tag="VLL03_03280"
     CDS             complement(2012..3196)
                     /gene="alaC"
                     /locus_tag="VLL03_03280"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HSS46419.1"
                     /translation="MQEFPRIERLPLYVFNITAELKAAARKRGEDIIDFGMGNPDGAT
                     PRHIVDKLIETVQRPDTHRYSVSRGIPRLRRAICNWYKARFNVEFDPDSEAIVTIGSK
                     EGISHLALATLDGGDIVLVPNPSYPIHIYGPVIAGADIRHVRITPGLDFFAELEKALK
                     DSYPKPKLLIINFPSNPTTQCVELLFFEKIIALAKEYGIYVVNDLAYADIVFDGYKAP
                     SIMQAPGARDVAVEFFTLSKSYNMPGWRVGFMVGNKKLISALARMKSYHDYGTFTPIQ
                     VASIVALEGPQDCVEEIRQTYQNRRDVLCKSLQAANWQVQTPKATMFVWARIPEAYRN
                     LGSLEFSKKLLADAKVAVSPGVGFGEHGDDHVRFSLIENEARTRQALRGIKDMFRKDG
                     LL"
     gene            3312..3680
                     /locus_tag="VLL03_03285"
     CDS             3312..3680
                     /locus_tag="VLL03_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HSS46420.1"
                     /translation="MKLHLATASAENRFTGYGEGYVLVNNVRHEKSLVVLPERLMEWE
                     VAAFEALSQKHFEFLMNLAPEIVLLGTGPRLRFPHPALSQSLVNAKIGLEVMDTRAAC
                     RTYNILVAEGRKVAAALLLG"
     gene            3716..4660
                     /locus_tag="VLL03_03290"
     CDS             3716..4660
                     /locus_tag="VLL03_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSS46421.1"
                     /translation="MQPHLLPQDSKISPPIDRVINQVSQIILGKDRQIRLALACLLAR
                     GHLLIEDLPGVGKTTLAHALARSLGLEFHRIQFTSDMLPADILGVSIFDRDTGAFKFH
                     PGPVFAQVVLADEINRATPKTQSALLEAMEEHQATTEGETRPLPEPFFVIATQNPSQF
                     VGTFPLPESQLDRFLMRIKLGYPDQQAERALLKGSERRDMITHLTPCMNMTELLELQQ
                     KVRAVHASDALTDYLQALINHTRQSPLFVTGLSPRAGLALLHSARAWALMHGRHQVLP
                     EDVQTVLPSVAGHRLHPAAEHARGKDLAESLINAVAIP"
     gene            4677..5654
                     /locus_tag="VLL03_03295"
     CDS             4677..5654
                     /locus_tag="VLL03_03295"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HSS46422.1"
                     /translation="MLLALKNHLNSILNTWLFRLKGPEAVAVLTQRRVFILPTRQGLL
                     FGLLLLLLLIGSINYNLSLGYIFTFLLAAMANLSMLHAFRNLAGLQVRPGKANPVFAG
                     EDASFGVCLDNQRLLPRYSIGIACNQREPVYCDVSPHAGTVAAVNLPSKRRGWLKPGR
                     FTLFTRHPLGLFRAWSYAEFDMRCLVYPQPEAAHLPPPLMQAKPGEGFEHGQGCDDFT
                     GLRPHQPSDSPRHVAWKAVARDHGLLTKQFIGRADAELWLDWNALPASLDEEQKLARL
                     CRWVLDAESAGLSYGLRLPEREIQPTRGELHQQQCLEALALFGLDDAAA"
     gene            5677..>6637
                     /locus_tag="VLL03_03300"
     CDS             5677..>6637
                     /locus_tag="VLL03_03300"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 domain-containing protein"
                     /protein_id="HSS46423.1"
                     /translation="MAGLVMVMVPHAERLPIWIPLAAGALMLWQFYIELRRQPLLPKW
                     LLFLLAIAAFAGIYLSYGRIFGRDTGVALLMLLVGLKLMETQTIRDATLVFFLGYFLV
                     ITNFLYSQTMATGLYMLLVVLVLTAALLELNRATGTYGVKQSFKLAGALLLQAIPLMV
                     VVFLLFPRIQGPLWGLPQDAYSGITGLSDTMSPGSLSELTLSDAVAFRVKFEASAPKN
                     ALLYWRGPVLWRFDGRTWSAGRFTSGEPPQWKGLGQSLRYTVTLEPHNKNWLFALDLA
                     AIAPPQAKISRDFQVLANAPVRNRLRYDVVSYLDYRAGVEEQAD"
BASE COUNT         1301 a         2009 c         1868 g         1459 t
ORIGIN      
        1 ccagcgagcc gtgcatcatc gcctgcgaaa gcttactcaa agcgatcttg ccttcgggga
       61 tcaccacgaa acacgcaatg cccgcacgcg ccgcatacgc cgctgctgcc gccgaagtat
      121 tgcccgttga ggcacagata atcgccttgg cgcctgcttc caccgctttg gtcacagcca
      181 gcgtcatgcc acggtccttg aacgaaccgg agggattcaa gccttcgaac ttggcgtaga
      241 gcctgacgtt ctttttaatc aaccgcggca ggttcttgag ctcgatcagc cgcgtaccgc
      301 cctcgcacag cgacacaaca ggtgtggccg cggaaaccgg tagacggcca cgatatcttt
      361 caattacacc actataacga ttgaccatat ttaatttacc gcggaggcgc ggagaaatca
      421 acccaatatc ttgatttttc tctgcgcctc ggcgtctctg cggttcaaac tcaattccta
      481 tccaactctt ccagccggat taaaacccta tttaccgcag aggacgcaga gagcgcagag
      541 aaagacaaaa caaaattatt ctgattttcc tcagcgtcct cagcgtactc cgcggttaaa
      601 aattctaatt tctatccaac tcttccagcc ggatgcgggt gaccttgccg gaaatggtgg
      661 aaagcttttc aattttcctg atcgcggcat tgatgcgttt ctccacagtt tcatgggtga
      721 gcatgatgat gtccacctgc tcctcgcctt cgctcggctc tttttgcacc atggcgtcaa
      781 tcgaaatccg cgaatcggcg agaatacggg taatgtcggc cagcaccccc ggcttgtcga
      841 aaacacgcag acgcagataa taagaagtct gcacttccga catcggcagg atgggcgtat
      901 cggtgagcgc atccggctga aacgcgagat gcggcacgcg gtgcttgcgg tacgcggtgt
      961 gcatgcgggt cacgtccacc aaatccgcca ccacggcgga agcggtcggt tccgctccgg
     1021 cgccggcgcc gtaatacagg gtggcgccca ccgcgtcgcc tttcaccagt actgcgttca
     1081 tcacgccttc cacgttggcg atgagacgcc gcgccggaat cagcgtcggg tgcacgcgca
     1141 attcaatgcc tttcggcgtg cgtttggtga tgccgaggag tttaatccgg tagcccagtt
     1201 cctcggcata acggatatcc tcgcgagtga gtttcgagat gccttcggtg taaaccttgt
     1261 cgaactgcat cggaatgccg aagctgatcg ccgccagaat ggtgagcttg tgcgcggagt
     1321 ctatgccctc gatgtcgaaa gtcgggtcgg cttcggcgta gcccagcgcc tgcgcctgct
     1381 tgagcgccgc gtcgaagctc acgcccttct cgcgcatctg cgagaggatg taattgctgg
     1441 tgccgttgat gatgccggcg atccactcca cgcggttggc agtcaggccc tcgcgcagcg
     1501 ccttgatgat gggaatgccg cccgccaccg ccgcttcgaa cgccaccatc actccgtgct
     1561 tctgcgcggc ggcgaaaatc tcgttgccgt gcttggccag cagtgccttg ttggcggtga
     1621 ccacatgttt gccgttctca atcgctttga gaatgaattg tttcgccgcc gtagcgccgc
     1681 caatcagctc caccacgatg tcaatctcgg gatgcctgat tacttccatc gcatcgcccg
     1741 tcaccacggc gtttttaccg gcgatcttgc gcgccttagc cacatccttg tcggccacca
     1801 tcctgatgac gatgccgcgc ccggcacgac gggtgatttc cttttggttg cgccgcagaa
     1861 cggtgaacgt gccgccaccc acggtaccga tgccgagcaa gcctacgtta attggtttca
     1921 taatttcaca ccttgttcac cgcagagacg cggagacgca gaggaatgtt atgttcgaaa
     1981 attcttctcc acgcctcggc gcctccgcgg ttcatagtaa accgtctttg cggaacatgt
     2041 ccttgatgcc gcgcaatgct tgtcttgtcc tcgcctcgtt ctcgatgagt gaaaaccgca
     2101 catggtcatc gccatgctcg ccgaacccga cgccgggcga taccgcgact ttggcgtcag
     2161 cgagcagctt cttggaaaac tcgagcgaac ccagattgcg ataggcttcg ggaatccgcg
     2221 cccaaacgaa catggtagcc ttgggcgttt gcacctgcca gtttgccgcc tgcaggctct
     2281 tgcacagcac gtcgcggcgg ttttgataag tctgacggat ttcctccacg caatcctgcg
     2341 gcccttccag cgccacgatg gaagccacct ggatcggcgt aaacgtgcca tagtcatggt
     2401 agctcttcat gcgcgcgagc gccgatatca gttttttatt gccgaccatg aagcccacgc
     2461 gccagccggg catgttgtag cttttcgaca gcgtgaaaaa ctccaccgcg acatcacgcg
     2521 cgcccggcgc ctgcatgatg gaaggtgcct tatagccatc gaacacgatg tcggcgtatg
     2581 ccaaatcatt caccacgtaa atcccgtatt ctttagccag cgcgatgatt ttttcgaaga
     2641 acaacagttc cacgcactgc gtagtcgggt tgctcggaaa attgatgatg aggagcttcg
     2701 gcttcggata cgaatccttg agcgcttttt ccagttcggc aaagaaatcg aggcccggcg
     2761 tgatgcgcac atggcggata tcggcgccgg cgattaccgg gccgtaaata tgaatgggat
     2821 agctcgggtt cggcaccagc acaatgtcgc cgccgtccag cgtcgccaag gcgagatgag
     2881 aaatcccctc tttcgagccg atggtgacaa tcgcttccga atcgggatca aactcgacat
     2941 tgaagcgcgc cttgtaccag ttgcatatgg cgcggcgcag ccgcggaatg cccctggaca
     3001 ccgagtaacg atgggtgtcc ggtcgctgca cggtttcaat cagcttgtcg acgatgtgcc
     3061 tgggcgtcgc gccgtcggga ttgcccatac cgaagtcgat gatatcctcg ccgcgcttcc
     3121 tcgccgccgc cttgagttcc gcggtgatgt tgaagacgta aagagggaga cgctcgatgc
     3181 gtggaaattc ctgcatattt tgtgacaaat attaaatttt caattttgtg acttatatca
     3241 aatctttagg tttgacaaaa aaggtataat cttacccgac cggtcaccct gccgcaattg
     3301 gacttgtgct gatgaaactg catcttgcca ccgcgagcgc cgaaaaccgt ttcaccggat
     3361 atggtgaagg ctatgtcctg gtcaacaacg tgcgccatga aaagagcctc gtcgtgctgc
     3421 ctgaacgcct gatggaatgg gaggtcgccg catttgaagc actcagccag aagcactttg
     3481 agtttctgat gaacctcgcg cccgaaatcg tgctgttggg aacgggtccg cgcttgcgtt
     3541 ttccgcatcc ggcactgtcg cagtcattgg ttaacgcaaa aatcggcctt gaagtcatgg
     3601 acacgcgcgc ggcctgccgt acttataaca tcctcgttgc ggaaggacgc aaggtcgccg
     3661 cggctttgct gctcggctga gaaaaacttt ttaacaaccc aaaatcgccc aattcatgca
     3721 accacatctg ctaccccaag actccaaaat ttcgccgccg atagaccgcg tcatcaatca
     3781 ggtgagccag attatcctcg gcaaggaccg gcaaatccgc ctggcgctgg cctgcctgct
     3841 ggcgcgcggc catttgctga ttgaagacct gccgggagtg ggcaaaacca ctctggcaca
     3901 tgcgctggcg cgctcgctgg ggctggaatt ccaccgcatc caattcacca gcgatatgct
     3961 gccggcggac attctcgggg tttcgatttt cgaccgcgac accggggcgt tcaagtttca
     4021 tcccgggccg gtgttcgcgc aggtggtgct cgccgacgaa atcaaccgcg ccacgcccaa
     4081 gacacaaagt gcgctgctgg aagcgatgga agagcaccaa gccaccaccg aaggcgaaac
     4141 gcgcccgctg cccgaaccct ttttcgtcat tgccacgcaa aatccttcgc aatttgtcgg
     4201 caccttcccg ctgccggaat cgcagctcga ccgctttctc atgcgcatca agctgggtta
     4261 ccccgaccag caggcggagc gcgcgctgct caaaggcagc gagcggcgcg acatgattac
     4321 ccacttaact ccctgcatga atatgaccga acttttggag ttgcagcaaa aagtaagagc
     4381 agtgcatgct tccgatgcgc tcaccgatta tttgcaagcg ttgattaacc acacccgcca
     4441 atcgccgctg tttgtgacgg gtttaagccc gcgcgcggga ctcgcgcttc tgcattcggc
     4501 gcgcgcctgg gcgctgatgc acggacgcca tcaggtgctg ccggaagacg tgcagacggt
     4561 gctgcccagc gtggccggcc accgtttgca ccccgcggcc gaacacgcgc gcggcaagga
     4621 tttggccgag agcctcatca acgcggttgc gataccttaa agtctggcta cgccagatgc
     4681 tgctcgcgct caaaaaccat ctgaacagca ttctcaacac ctggctgttc cggctcaaag
     4741 ggccggaagc cgttgcggtt ctcacccagc gccgcgtatt cattctgccc acccgccagg
     4801 ggctgctgtt cgggctgttg ctgctgctgc tcctcatcgg ctcaatcaat tacaacctga
     4861 gcttgggtta tatcttcacc ttcttgctag cggccatggc gaacctgtcc atgctgcacg
     4921 cttttcgcaa tctggcggga ctgcaggtgc gccccggcaa agccaatccg gttttcgccg
     4981 gagaagacgc gagtttcggc gtgtgcctgg acaatcagcg cctgctgccg cgctactcca
     5041 tcgggatagc ttgcaaccag cgggaaccgg tctattgcga tgtctcgccc catgccggca
     5101 ccgtggcggc agtaaacctt ccatcaaagc ggcgcggctg gctcaagccc ggacgtttca
     5161 cgctgttcac gcgccatcct ctcgggttgt ttcgcgcctg gtcgtatgcg gagttcgaca
     5221 tgcgctgcct ggtttaccct cagcctgaag ccgcgcattt gccgccgccc ttgatgcaag
     5281 ccaagccggg ggaaggcttc gagcatggcc agggctgcga cgatttcacc ggcttgcgtc
     5341 cacatcagcc cagcgattca ccgcggcatg tcgcatggaa agcggtggcg cgcgaccacg
     5401 gcttgctcac caagcaattc atcggccggg cggacgccga gctttggctc gactggaacg
     5461 cgctgcccgc ctcgctcgat gaggaacaga agcttgcgcg cttgtgccgc tgggtgctgg
     5521 acgcggaaag cgcgggctta agctatggcc tgcgcctgcc ggagagagaa atccagccga
     5581 cgcgcggcga gctgcaccag cagcaatgcc tggaagcgct ggccttgttt ggactggatg
     5641 atgcagcggc ttgaattacg ccattgggtt tggcttttgg cgggcctggt catggtgatg
     5701 gtgccccatg ctgaacggct gccaatatgg attccgctcg ccgccggcgc gcttatgctg
     5761 tggcagtttt atatcgagtt gcgccggcaa cctttgttgc cgaaatggct gctgttcctg
     5821 ctggctattg ccgcttttgc cgggatttat ttaagctacg gcaggatctt cgggcgcgat
     5881 accggtgtgg cgctgctgat gctgctggtg gggctcaagc tcatggaaac ccaaaccatc
     5941 cgcgacgcca cgctagtttt cttcctcggc tattttctgg tcatcaccaa ttttctctac
     6001 tcgcaaacca tggccaccgg gctttacatg ctgttggtag tgctggtgct gaccgcggcg
     6061 ctcttggaac tcaaccgtgc gaccggcacc tatggcgtga agcaaagctt caagcttgcc
     6121 ggcgccctgc tgcttcaggc aataccgtta atggtggtgg tgttcctgct tttcccgcgc
     6181 atacaaggcc cgctgtgggg tttgccgcag gacgcttatt ccggcatcac cggtttaagc
     6241 gacaccatgt cgcccggctc gctgagtgag ttgaccctgt ccgacgcggt ggcgttccgc
     6301 gtcaaattcg aagcgtccgc gcccaagaat gccttgctgt attggcgcgg gccggtgttg
     6361 tggcgcttcg acggccgcac ctggtcggcg ggacgattca cttcaggcga gccgccgcaa
     6421 tggaaaggct taggccaatc gctgcgctac acggtgacgc tggaaccgca taacaagaac
     6481 tggttgtttg cgttggatct ggccgccatc gcgccaccgc aggcgaaaat ttcccgtgat
     6541 tttcaggtgc tggcgaatgc tccggtgcgc aaccggctgc gctatgacgt ggtgtcttat
     6601 ctcgactacc gcgcgggcgt ggaggagcag gcggatg
//
LOCUS       DATIOW010000102         3319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_151782, whole genome shotgun sequence.
ACCESSION   DATIOW010000102 DATIOW010000000
VERSION     DATIOW010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151782"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VLL03_03305"
     CDS             complement(<1..546)
                     /locus_tag="VLL03_03305"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSS46424.1"
                     /translation="MNAISEARLLESQLRITEIFYSLQGETSRVGLPTVFIRLTGCPL
                     RCGYCDTEYAFTGGETMTLAEIMAQVTAHQAHYVTVTGGEPLAQKNCLLLLRELCEAG
                     YSVSLETSGALDVAEVDPRVSKILDIKTPGSGEVEKNLWRNLVHLSPRDEIKFVLCDE
                     EDYKWAAKQLHERNLAEICPVL"
     gene            complement(702..1475)
                     /gene="ybgF"
                     /locus_tag="VLL03_03310"
     CDS             complement(702..1475)
                     /gene="ybgF"
                     /locus_tag="VLL03_03310"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HSS46425.1"
                     /translation="MRALAAGLCALVFATPCFAGLFSDEEAHKKIAQLQQQILELRSQ
                     NQALESRILKLEEILKNQGLLDLLSQIESLKADVNKLRGQIEVQNNSVESLEKRQKDF
                     YIDLDSRLRRMEQPSQALAPPDAAGTNKPAGGGETQGYEAAYNLFKIGNYQGTISAFQ
                     NFLKNYPSSNLASSAQYWIGNAYFALRDFKNAIASQQKLLSTYPDSQKAPDAMLNIAS
                     SQLESGDSAAARKTLDELIAKYPVSDAAEKAKRRLSTIK"
     gene            complement(1482..2009)
                     /gene="pal"
                     /locus_tag="VLL03_03315"
     CDS             complement(1482..2009)
                     /gene="pal"
                     /locus_tag="VLL03_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HSS46426.1"
                     /translation="MKKILLSILLASLLAACASQEAKEQSKAAVEERTPTQTTAPTPQ
                     TAPAQRESVAVDPLHDPSNILSRRSVYYDFDSSSIKDEYRPLVEAHARYLSGNRSARV
                     TVQGNCDERGSREYNLALGQRRADGVKKAMTVLGASSSQIQTVSFGKEKPKCTEHGES
                     CWSQDRRSDIVYQGE"
     gene            complement(2043..>3319)
                     /gene="tolB"
                     /locus_tag="VLL03_03320"
     CDS             complement(2043..>3319)
                     /gene="tolB"
                     /locus_tag="VLL03_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HSS46427.1"
                     /translation="ALPKIIFLLLLLCASPLQAALTIEIIGGGATQIPITIVPFAAEQ
                     GLPQSITAVVGADLARSGLFKLVDTGGINPLPVEPSDIRYGDWKARGADAMVIGSIRQ
                     LPDAKIEVRFRLMDVTKQAQLAGFSYTILPAQLRITAHKIADVVYEKLTGDRGVFSTQ
                     ISYVVKQGNHYELQVADADGYGSQTVVASNDAIISPAWSPEGSRIAYVSFEKKRAVTY
                     VQSLTTGKRTLLAGFEGSNSAPAWAPDGKRLAIVLSKDGISQIYLVNPDGSGLQRITF
                     SGAIDTEPNFSSDGKWIIFTSDRGGSPQIYRMPAGGGEAERLTFEGTYNVSPHFSPDG
                     RSFVFIQRGGSRFNVAVEDLATRQAQVLTDSILDESPSYAPNGKIILYASEAGGRGIL
                     AAVSSDGKVKQRLTVPSGDVREPSWGPFLKAQ"
BASE COUNT          664 a          954 c          962 g          739 t
ORIGIN      
        1 cagcaccgga cagatttcag cgagattgcg ctcatgcaat tgttttgccg cccacttata
       61 atcttcctcg tcgcacagca caaacttgat ttcatcgcgc ggcgaaagat gcacaagatt
      121 gcgccaaaga tttttttcta cttcgccgga acccggggtt ttgatgtcga ggattttgga
      181 aacgcgcgga tcaacctcag cgacgtccag cgcgccgctg gtctccagcg acaccgaata
      241 acccgcttcg cacagctcac gcagcaaaag caggcaattc ttttgcgcca gcggctcgcc
      301 gccggtaacc gtcacataat gcgcctgatg cgccgtaacc tgcgccatga tttcggcaag
      361 cgtcatcgtc tcgccgccgg tgaacgcata ttcggtatca cagtagccgc agcgcaatgg
      421 acagccggtt aaacggataa aaaccgtagg caagccgacg cggctggttt cgccctgcag
      481 ggaataaaag atttctgtaa tgcgcagttg gctttccagc aagcgcgctt cagagattgc
      541 attcataaga ataaacacta ccccgaaacc gagtgcgaaa caagaagtct ggcttcaggt
      601 aaggcgccgc gaaattttgt ggcggagccg acagcatgtg agcaagcaaa acaagcggca
      661 acgcagcatg aaggcaaaat ccgcggtttc tcagaggttt cttacttgat tgtgctgagg
      721 cggcgcttag ccttttccgc ggcgtcgctt accggatatt tggcaatcag ttcatccagt
      781 gttttgcgcg cagccgcgct gtcgcccgat tccagctgcg agctggcgat attgagcatg
      841 gcatccggcg ctttctggct gtccgggtag gtgctgagca gcttctgctg gctggcaatc
      901 gcgttcttga aatcgcgcaa ggcgaaatag gcattgccga tccagtattg cgcgctggac
      961 gcaagattgc ttgaggggta atttttaaga aagttctgaa aagcggaaat ggtaccctga
     1021 taattgccga ttttgaaaag attgtatgcc gcttcgtagc cctgggtttc gccgccgccc
     1081 gcgggcttgt tcgtccccgc agcatcggga ggcgcaagcg cctgcgacgg ttgctccatc
     1141 cggcgcagcc ggctgtcgag atcgatgtaa aaatccttct ggcgtttctc cagggattcg
     1201 acgctgttat tctggacttc aatttgcccc cgcagcttgt tgacgtctgc cttgagggat
     1261 tcaatctgac tgagaagatc gaggaggccc tggtttttca ggatctcctc gagtttcagg
     1321 atgcgcgatt ccagcgcctg gttctgactc cggagctcca gaatctgctg ctgcagctgg
     1381 gcgattttct tgtgcgcctc ttcatcgctg aacaggccgg caaaacaagg cgtcgcgaaa
     1441 acgagggcgc acaggcccgc tgccaaagcg cgcaaaacgg attactctcc ttgatagacg
     1501 atgtcgctgc ggcggtcctg cgaccagcaa gattcgccat gctccgtgca cttgggtttc
     1561 tccttgccga agctcacggt ctggatttgg ctcgacgatg cgcccagaac agtcatcgcc
     1621 tttttcacgc cgtccgcacg gcgctggccg agcgccaggt tgtactcgcg gctgccgcgc
     1681 tcgtcgcaat tgccttgaac ggtgacccgc gcgctgcggt tgccggagag ataccgggca
     1741 tgggcctcaa ccagcggccg atactcgtcc ttgatcgagg agctgtcgaa atcgtaataa
     1801 acgctgcgcc gtgaaagaat attactcggg tcatgcagtg gatcgactgc gacggattcc
     1861 cgctgtgccg gcgccgtctg aggggtggga gcagtggttt gagtcggcgt tctttcttcc
     1921 accgccgcct tcgattgctc cttggcttcc tgactcgcgc aagcggcaag caggcttgcc
     1981 aacagtatgc tcaataggat tttcttcact tttgtctcct ttacactggt tggaataggg
     2041 tcttactggg cttttaaaaa cgggccccat gaaggttcgc gcacgtcgcc cgatggcacg
     2101 gtgagcctct gtttcacttt accatcactt gaaaccgctg ccaatatgcc gcggccgcca
     2161 gcttcgctgg catagagaat aatcttgcca tttggcgcgt aactgggtga ttcatcgagg
     2221 attgaatcgg tgagcacctg cgcctgacgg gttgccagat cctccaccgc cacgttgaag
     2281 cgactgccgc cgcgctggat gaatacaaag cttctgccgt ccgggctgaa atgcggagac
     2341 acgttataag tcccctcgaa agtcaggcgc tcggcttcgc cgccgccggc cggcatgcgg
     2401 taaatttgcg ggctgccgcc gcggtctgag gtgaaaatga tccacttgcc gtcgctggaa
     2461 aagttgggct cggtgtcgat cgctccgctg aatgtgatcc gctgcaggcc gctgccgtcg
     2521 gggttgacga gataaatctg cgagatgccg tccttgctaa gcacaatggc gagccgcttg
     2581 ccgtccgggg cccacgccgg cgcgctgttg ctgccctcga agccggcaag cagcgtgcgc
     2641 ttaccggtag tgagcgactg cacataagtc accgcccgtt ttttctcgaa cgaaacatag
     2701 gcaatcctgc tgccttcggg cgaccaagcg ggggaaataa tggcgtcatt tgaagccacc
     2761 actgtttgtg agccgtagcc gtcggcatcg gcgacctgca gctcgtagtg gttgccctgc
     2821 ttcaccacat agctgatctg ggtgctgaac acaccccggt cgccggtgag tttctcatac
     2881 accacgtcgg caatcttgtg cgcggtgatc cgcaattgcg ccggcaagat ggtgtagctg
     2941 aatccggcaa gctgcgcctg tttcgtcaca tccatcagac ggaagcgcac ctcaatcttg
     3001 gcgtcaggga gctgtctgat ggagccgatg accatggcat ccgcgccgcg cgctttccag
     3061 tcgccgtagc ggatgtcgga aggctccacc ggcagcgggt tgatgccgcc ggtgtccacc
     3121 agtttgaaca ggccgctcct ggccaaatca gcgccgacca ccgcggtgat gctttgcggc
     3181 aaaccctgct ccgcggcgaa aggtacgatg gtaatcggga tttgcgtcgc gccgccgccg
     3241 atgatttcaa tggtgagcgc ggcctgaagc ggacttgcgc aaagcaacag cagcaggaaa
     3301 attatttttg gcaaagcgc
//
LOCUS       DATIOW010000103         3304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_455571, whole genome shotgun sequence.
ACCESSION   DATIOW010000103 DATIOW010000000
VERSION     DATIOW010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455571"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1184)
                     /gene="hisC"
                     /locus_tag="VLL03_03325"
     CDS             complement(111..1184)
                     /gene="hisC"
                     /locus_tag="VLL03_03325"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HSS46428.1"
                     /translation="MAKSPEQLIRDDVRALSAYHVPESAGLVKLDAMENPHALPAPLQ
                     AEIAQLVSRAAINRYPDPQASRLKARLREAMEIPSGMEIIVGNGSDELIQMIAFAVAK
                     PGATLLSVEPAFAMFRLIAICAGLRYAGVQLNPDFTLDVEAVITAINQHQPTVIFIAY
                     PNNPTGNLFDREELSRIIKAAPGLVVVDEAYHAFAEESFMPRLTDHPNLLVMRTLSKL
                     GMAGLRLGFLVGRTEWITQLEKLRLPYNVNVLTQAVAEKLLQRMDVLLEQAREIKSQR
                     AKLYAELSALPGVQAYRSDANFLLFRVSGADEVFKGLIQRGILIKNLNHSHPLLDDCL
                     RATVGTAQQNRQFIQALKESLIP"
     gene            complement(1205..2503)
                     /gene="hisD"
                     /locus_tag="VLL03_03330"
     CDS             complement(1205..2503)
                     /gene="hisD"
                     /locus_tag="VLL03_03330"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HSS46429.1"
                     /translation="MAKLRRLSTTQRDFERRLKALLAFEAVLDAKVDTAVVRILAEVK
                     ARGDAALLEYTRRFDKLKAKTVRELELPRASLAAALLTLPNPQRQALEEAASRIRAYH
                     EKQLVQSWSYRDADGTLLGQQVTSLERVGMYVPGGKAAYPSTVLMNAIPARVAGVREL
                     IMTVPTPYGERNDLVLAAAAICGVDRVFTIGGAQAIGALAYGTKTSPQVDKIVGPGNA
                     YVAAAKRRVYGVVGLDMVAGPSEILVICDGQTDPDWVAMDLFSQSEHDDMAQAILLSP
                     DAKFMDAVAESIKRLLPSLPRREVIRASFEKRGALIEVKNLDEACAIANRIAPEHLEL
                     SVENAESWAAKIKNAGAIFIGRYSSEALGDYCAGPNHVLPTSRSARFSSPLGVYDFQK
                     RSSVISISKEGAVKLGKVAVELAMGEGLQAHVRSAQYRMK"
     gene            complement(2496..3143)
                     /gene="hisG"
                     /locus_tag="VLL03_03335"
     CDS             complement(2496..3143)
                     /gene="hisG"
                     /locus_tag="VLL03_03335"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HSS46430.1"
                     /translation="MAGITLALSKGRIFDETLPLLKAAGIVALNGPEISRKLILASNR
                     ADIRIIIVRASDVPTYVQHGAADLGIAGKDVLDEHGGAGLYQPLDLNIGRCHMMVAVP
                     AGFDYASAVRQGARLRVATKYLQAAREHFAAKGVHVDLVKLYGSMELAPLTGLADAIV
                     DLVSTGNTLKANNLQAVEEIAPVSARLIVNQAALKLKREAIRPLIDAFEKAVSRG"
BASE COUNT          688 a          981 c          912 g          723 t
ORIGIN      
        1 ttcgtcttgc cgccgccgtc gagattgagt ttcacgccga ttttagtttc cagggtattg
       61 cgggtaactt gggccttgcg catgggtgcc ttgttgaaaa aaatagtatc ttaggggatg
      121 aggctttctt tgagcgcctg aatgaattgc cggttttgct gtgccgtgcc caccgtcgcc
      181 cgcaagcagt catccagcag cgggtggctg tggttgagat tcttgataag gattccgcgt
      241 tgaattaagc ctttaaacac ctcatctgcc ccggataccc ggaacagaag gaaattcgca
      301 tcgctccgat acgcctgcac tcctggcagc gctgacaatt ctgcatatag ttttgcgcgc
      361 tgcgacttga tttcccgcgc ctgctcgagt aacacatcca tgcgctgcaa aagtttttcg
      421 gcaacggctt gtgtcagcac attcacatta tacggcaaac gcagcttttc cagttgcgta
      481 atccactcag tgcgccctac tagaaagcca agcctcaatc ccgccattcc cagtttggaa
      541 agcgtacgca tcaccagcag attagggtgg tcagtaagac gcggcatgaa actttcttca
      601 gcaaacgcat gatacgcttc atccaccacc accaggcccg gtgccgcctt aatgatacgc
      661 gacagctctt cacggtcaaa cagattcccc gtcgggttat tgggataagc aataaaaatc
      721 actgttggct ggtgctggtt gatcgccgtg ataaccgctt cgacatcaag cgtgaagtcg
      781 ggattaagct gcacgcctgc ataacgcagc ccggcgcaga ttgcaatcaa gcggaacatc
      841 gcgaaggcgg gttccacgct caacaaggtt gctccgggct tggcgaccgc gaaagcgatc
      901 atttgaatca gctcgtcgga accgttgccg actatgattt ccatgcccga aggaatttcc
      961 attgcttcgc gcaaccgcgc tttcagccgg cttgcctgcg ggtcgggata gcggttgatg
     1021 gcagcgcggc tcaccaattg cgcaatctcg gcttgcaacg gcgcaggcaa agcgtgcggg
     1081 ttctccatcg cgtcgagctt caccaatccc gcgctctccg gcacgtgata agcgctcaac
     1141 gcacgaacat catcgcgtat gagctgttcg ggagatttag ccatggttga aatgcgctgc
     1201 accgctattt catgcggtat tgggcggacc tgacgtgtgc ctgcaaaccc tctcccatgg
     1261 cgagttccac cgccactttg ccgagtttta ccgcgccttc cttggatatg ctaatcacgc
     1321 tggagcgctt ctggaaatcg tacacgccca acggcgagga aaagcgcgcg ctgcgcgaag
     1381 tcggcagcac gtgattcggc ccggcgcagt aatcgcccaa agcctccgag ctgtaacggc
     1441 cgataaaaat cgcgccggca ttcttaattt tggccgccca gctttctgcg ttttccacag
     1501 agagctcgag atgctcaggc gcgatgcggt tggcaattgc acaggcttca tcgagatttt
     1561 tcacctctat cagcgcaccg cgcttttcga aggaagcacg gataacttcc cggcgcggca
     1621 gcgaaggcag caggcgtttg atgctctctg caaccgcatc catgaatttt gcgtcaggtg
     1681 aaagcaagat agcctgcgcc atatcgtcgt gctcggactg agagaacaaa tccatcgcca
     1741 cccaatcggg atcggtctgg ccatcgcaaa tcaccagaat ttcagaaggc cccgctacca
     1801 tgtccaggcc taccacgccg tacacgcgcc gcttcgccgc ggcgacataa gcattgcccg
     1861 ggccgacgat tttgtctact tgcgggctgg tcttggtgcc ataagccagc gcacctattg
     1921 cctgtgcgcc accgatggta aacacgcgat ctacaccgca gattgcggca gcggcaagca
     1981 ccaaatcgtt gcgctcacca tagggagtcg gaactgtcat gatgagctcc cgcactcccg
     2041 ccactctggc gggaatcgca ttcatcagca cggtggaagg ataagccgcc ttgccgccgg
     2101 gaacatacat gcccactcgc tcgagagatg tcacttgttg gccaagcagc gtgccgtcgg
     2161 catcgcgata gctccaggat tggacaagct gcttttcgtg atacgctcgg atacggctcg
     2221 ccgcctcttc cagtgcttgc cgttgaggat taggaagggt gagtaatgcc gcggccaggc
     2281 ttgcccgcgg cagttccagc tcgcgcactg ttttggcctt gagcttgtcg aagcgccgcg
     2341 tgtattcgag caatgcggca tccccgcgcg cttttacctc agcgagaatt ctgaccacgg
     2401 cagtatccac cttggcatcg agcaccgctt caaatgcaag cagcgcctta agcctgcgtt
     2461 caaaatcgcg ctgggtggtc gaaagacgcc tgagcttagc cacggctcac cgctttttca
     2521 aatgcgtcaa tgagcggcct gattgcctcg cgtttcagtt tcagcgccgc ctggttgact
     2581 atgagcctgg cggaaaccgg cgcgatttct tccaccgcct gcaggttgtt ggctttcagc
     2641 gtgttgccgg tgcttactag atccacgatt gcgtcggcca gcccggtgag cggggcgagc
     2701 tccatcgagc cgtagagctt gacgagatca acatgcacgc ctttcgccgc aaagtgctcg
     2761 cgcgcggctt gcaggtattt ggtggcgacg cgcagccgtg cgccctgccg caccgcgctt
     2821 gcataatcaa aacccgcggg caccgccacc atcatgtggc agcggccgat gttcaaatcc
     2881 agcggctggt acagcccggc tccgccgtgc tcatccagca cgtccttacc ggcgatgccc
     2941 agatccgcag cgccgtgttg aacgtatgtg ggcacgtcgg acgcgcgcac gatgatgatg
     3001 cggatgtccg cgcggttaga ggcaagtatc agcttacgcg agatttccgg accattgagc
     3061 gcgacaatgc ccgccgcttt caagagcggc aaggtctcgt cgaaaatgcg gcctttggat
     3121 aaggcgaggg taatgcctgc cacggcaatc tcttaatcaa aaaagcgcat tttaccgcag
     3181 agaggcggca gcgcaggaat ttttctttga aaatcaatgc gcgtggcgga tgcgggcacc
     3241 gagctgcgac agtttttctt ctatgcactc atagccgcgg tcgagatgat agacacgctc
     3301 cacc
//
LOCUS       DATIOW010000104        16171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1269800, whole genome shotgun sequence.
ACCESSION   DATIOW010000104 DATIOW010000000
VERSION     DATIOW010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16171
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269800"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VLL03_03340"
     CDS             complement(<1..195)
                     /locus_tag="VLL03_03340"
                     /inference="COORDINATES: protein motif:HMM:NF015575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HSS46431.1"
                     /translation="MANKRTAFFISDRTGITVEMLGHSLLTQFDGVDFRQITVPFVDT
                     VEKARETANHINQTGAADGSR"
     gene            273..2648
                     /gene="ppsA"
                     /locus_tag="VLL03_03345"
     CDS             273..2648
                     /gene="ppsA"
                     /locus_tag="VLL03_03345"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HSS46432.1"
                     /translation="MEQFIAWFERLRMSDVGSVGGKNASLGEMISQLAGSGVRVPGGF
                     ATTAKAFREFLALNGLQTRIKSSLRMLDINDVAVLAATGAKIRQWIADAPFQPKLEKE
                     IREAYRKMTAQAKADISVAVRSSATAEDLPDASFAGQQETFLNIRGEENVLAAIKSVF
                     ASLYNDRAISYRAHQGFAHSEIALSAGVQRMVRSDLGASGVMFTLDTESGFDQVVFIT
                     SSYGLGETVVQGAVNPDEFYVYKAGLRQNRPAIIRRNLGAKAVKMVLAERQEAGHSVK
                     TLEVIPQQRSSFSISDAEVEELARYALIIEQHYKRPMDIEWGRDGVDGKLYILQARPE
                     TVKSRGRVEILRRYRLKQRSEILASGRAIGQKIGSGPVRLIMDASEMSRVQPGDVLVT
                     DMTDPDWEPVMKRASAIVTNRGGRTCHAAIVARELGIPAVVGCGDATRTVKEGEAVTV
                     SCAEGDTGNVYKGMLEIEIMDLSLDALPKLPVKIKMNVGNPELAFDFQRLPNAGIGLA
                     RLEFIIARMIGVHPRAALDYPDIPAELRGQVEERSAGYPDPVSYYVEKIVEGVATLAA
                     AFWPKPVIARLSDFKSNEYSSLIGGQRYEPHEENPMIGFRGASRYIADSFRDCFALEC
                     RAFKRVRNDMGLTNVEIMVPFVRTLDEAAQTIELLKQNGLQRGVNGLRVIMMCEVPSN
                     AILADEFLQYFDGFSIGSNDLTQLTLGLDRDSSLVEGGFDECNPAVKKMLHLAIQACR
                     RAGKYVGICGQGPSDHPDLVRWLMEEGIESVSLNPDSVIETWLYLAGEAAK"
     gene            2650..3093
                     /locus_tag="VLL03_03350"
     CDS             2650..3093
                     /locus_tag="VLL03_03350"
                     /inference="COORDINATES: protein motif:HMM:NF018908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HSS46433.1"
                     /translation="MLDIRLKPSGYLILWLGATHAIALGLFLVLSLPVWPKLVATLFF
                     CISLVFCLKRHAWLTAPSSIIALEIKEDCTCVIETRSGKRLDCILLPTSYVSASLTVL
                     NFKANGERLARHAVLLPDAINPEDFRKLRILLRWKFKAKPLAPIT"
     gene            complement(3086..4687)
                     /gene="nadB"
                     /locus_tag="VLL03_03355"
     CDS             complement(3086..4687)
                     /gene="nadB"
                     /locus_tag="VLL03_03355"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HSS46434.1"
                     /translation="MRRFDVLIIGSGLAGLTVALNLADRKKVGLVTKKALIDGASSWA
                     QGGIAAVLGDDDSIAAHIQDTLVAGAGLCDEAATRYVVEHGRESIQWLINQGVPFTRD
                     EESEIGYHLTREGGHSHRRVIHAADATGRAVQTTLEAKIKAHPDITVLEHHIAVDLIT
                     GRKLGLPDNRCYGIYVLDSVADHVETIAADHTVLATGGAGKVYLYTTNPDTAAGDGIA
                     MGWRAGCRVANMEFIQFHPTCLYHPHAKSFLISEAVRGEGGLLRLPDGTRFMPEHDQR
                     AELAPRDIVARAIDFEMKKRGLDCVYLDISYKPSDFVREHFPTIHARCLALGIDITRQ
                     PIPVVPAAHYTCGGIVTDLQAHTDIQGLYAVGETGHTGLHGANRLASNSLLECLVFGR
                     TAAQDILRQPAGIRIALPAWDESRVTDADEEIVISHNWDELRRFMWDYVGIVRTSKRL
                     ERAQHRIRLLHEEINEYYANFRVSNDLLELRNLVMTADLIVRSARLRKESRGLHFSRD
                     YPLALPEARNTVLQLRAEAAALPVM"
     gene            4932..5531
                     /gene="rpoE"
                     /locus_tag="VLL03_03360"
     CDS             4932..5531
                     /gene="rpoE"
                     /locus_tag="VLL03_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HSS46435.1"
                     /translation="MGDREVDQRLVERAQHGDKHAFELLVVKYQRKLARLLSRFIRDA
                     AEIEDVTQEAFIKAYRALPAFRGDSAFYTWLYRIGINTAKNYLVAMGRRAPTTTGFDS
                     EEAETFEDGDLLRDVNTPENELMSKEIGKTVNQTLLELPEELRTAITLREIEGLSYED
                     IATIMNCPIGTVRSRIFRAREAIAEKLRPLLGTSKDKRW"
     gene            5534..6052
                     /locus_tag="VLL03_03365"
     CDS             5534..6052
                     /locus_tag="VLL03_03365"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HSS46436.1"
                     /translation="MKQDISGLMDGELDPDAATAVIARLKHEEDLKNAWADYHLIGDA
                     LRQDATLFLGFSERLSEKLGHEPAILAPRRTLAQKVKFTALSVAASLAAVAMVGWVVL
                     QNYTDKPKENLAATQPTSAQAMLASLPRYPFNSNVNEYLLAHQEFSPSTTMQGVVSYA
                     RTVSGEGREASR"
     gene            6049..7014
                     /locus_tag="VLL03_03370"
     CDS             6049..7014
                     /locus_tag="VLL03_03370"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HSS46437.1"
                     /translation="MKAVLFALLLWPAALLADTAPDEALAWLQKIASAARELNYNGTF
                     VYQYGNHVETSRIVHAVDGSGEQEKLEILDGLPREIIRSNDEVRCYLPESKTVMVEKR
                     THRTKSFPALLPEQLNNLNESYLIKMGGHERIAGFDCQALILEPRDGLRYGHKFWAEM
                     NSGLLLKASMLDENNEVVEQFAFTQLSIGGPIDKEMLKPRFSAKTPEWRYDHAAQAEP
                     FSTSTGWAIRPPPAGFKKIMETRRVISGKSAPVSHIVYSDGLAAVSVFIEPLGPKTKS
                     MNGLQKREALNVYTKPVADFQITVLGETPPITVMEIGNSVYYSGK"
     gene            7112..8488
                     /locus_tag="VLL03_03375"
     CDS             7112..8488
                     /locus_tag="VLL03_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HSS46438.1"
                     /translation="MKKLLWAFVLMFSFAVSGFAKELPDFTDLAEKQGLAVVNVSTMQ
                     TVRSQGVPQLPFSEDDPFYEFFRRFAPPMPREFESRSLGSGFIISADGYVLTNAHVVD
                     SADEITVKLTDKREFKAKVIGSDKRTDVALIKIDAASLPKVTLGDPNKLKVGEWVVAI
                     GSPFGFENSVTAGIVSAKGRSLPQENFVPFIQTDVAINPGNSGGPLFNLKGEVVGINS
                     QIYSRTGGFMGLSFAIPIDVAMEVADQLRTTGKVSRGRIGVVIQEVTKELAESFGLSK
                     PTGALINSVEKGGPADKAGLEPSDVILKFDGKSVSNSADLPRIVGATKPGSKVTVQVW
                     HKGAAKDINLTVGEISSDDKTAQRQGKYGKEPDTRYGMLLDNLTPDQRSELGIKGGVL
                     VEDVQGAAARAGIRRGDIILAVNNQDVKSVEHFNQLLSKYEKGRSVAFLIRRGDNALY
                     IPLRINGS"
     gene            8478..8747
                     /locus_tag="VLL03_03380"
     CDS             8478..8747
                     /locus_tag="VLL03_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HSS46439.1"
                     /translation="MAAESRRKLTVLSREYCHLCEDMVAALRQLQAQRGYFDLEVVDV
                     DCDAALEERYGERVPVLLLDGVEVCHYRFDPEVLDAHLIAVTRQA"
     gene            8813..10606
                     /gene="lepA"
                     /locus_tag="VLL03_03385"
     CDS             8813..10606
                     /gene="lepA"
                     /locus_tag="VLL03_03385"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HSS46440.1"
                     /translation="MNLIRNFSIIAHIDHGKSTLADRFIQICGGLSEREMEEQVLDSM
                     DLERERGITIKAQTAALNYKARDGQDYLLNLIDTPGHVDFSYEVSRSLAACEGALLVV
                     DVSQGVEAQTVANCYTALEQGVEVVPVLNKMDLPSADPERVIAEIEDIIGIPAKGALR
                     VSAKTGLGVEEILESVIRRIPPPKGKLDAPLKALIIDSWFDNYVGVVMLVRVVDGTLK
                     PKNKVLLMSNQATYLCEEVGVFTPKPKTKDSLSAGEVGFVIAGIKELSAAKVGDTLTL
                     AVSPAPAPLPGFKEIKPQVFAGLYPVDSNQYDALRDALEKLKLNDASLHYEPETSQAL
                     GFGFRCGFLGLLHLEIVQERLEREYGMNLITTAPTVVYQVLLRNGEVLEIENPSKMPD
                     PSKIEEVREPIITATILVPQDYVGPVITLCIEKRGVQKNMQYLGRQVLLTYELPLNEV
                     VMDFFDRLKSVSRGYASLDYEFEAYRAADLVKLDVLINGDRVDALSLIMHRANSQYRG
                     RELAVKMRELIPRQMFDVAVQAAIGSHIIARETIKAMRKNVLAKCYGGDITRKKKLLE
                     KQKAGKKRMKQVGNVEIPQEAFLAILRVESK"
     gene            10610..11413
                     /gene="lepB"
                     /locus_tag="VLL03_03390"
     CDS             10610..11413
                     /gene="lepB"
                     /locus_tag="VLL03_03390"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HSS46441.1"
                     /translation="MNFALVMFILLIGTGGITLLDRLWLKSRRRPDRTEPWWVEYPKS
                     FFPVILVVFLLRSFLVEPFKIPSGSMIPTLLVGDFILVNKFTYGIRLPILNAKIIDID
                     EPRRGEVMVFRYPENPSLDYIKRVVGLPGDRVVYRNKRLWINGEEIKITPSGEYNYVE
                     SGLNFITAKRFTENLGGHRHTILVQKEAPAVQTGGVRPFPFRENCVYNEEGFACTVPA
                     GHYLMLGDNRDSSSDSRYWGFVPQGNIVGKAFMIWWNFDNLKRIGQSIE"
     gene            11422..11784
                     /locus_tag="VLL03_03395"
     CDS             11422..11784
                     /locus_tag="VLL03_03395"
                     /inference="COORDINATES: protein motif:HMM:NF027463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HSS46442.1"
                     /translation="MKRQQGLTMTGFLFLAIALVFAALLGFKVLPAYLEYFSIQRALS
                     GMAHDLDLQTATPKEIRSSFDNRAAIDNITTITGQDLEIDKEGNRAVVRAIYAQKIPL
                     FANISAYIEFQASTDKRK"
     gene            11804..12475
                     /gene="rnc"
                     /locus_tag="VLL03_03400"
     CDS             11804..12475
                     /gene="rnc"
                     /locus_tag="VLL03_03400"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HSS46443.1"
                     /translation="MDIESVCRKLGYSFRQTELLRQSLTHRSFGSPHNERLEFLGDSI
                     LNCVIAHALFRRFPALSEGELSRLRANLVNQQSLFELAQKLNLGDEVLLGEGELKSGG
                     FRRPSILADTLEAMIAAVFLDGGFDQAGQVVQNLYATLLQDIDPKNPGKDPKTLLQEF
                     LQSRKMALPQYSVVATAGEAHQQQFRVECLVAELNIRSFGTGTSRRNAEQDAARQAYQ
                     LAAGA"
     gene            12479..13375
                     /gene="era"
                     /locus_tag="VLL03_03405"
     CDS             12479..13375
                     /gene="era"
                     /locus_tag="VLL03_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HSS46444.1"
                     /translation="MPGSIHNRYRSGLIAVVGRPNVGKSTLLNRLIGQKISITSRKPQ
                     TTRHRITGILTREDAQYIFVDTPGFQTRHGGALNRILNRSVVQSLDAADAVLFVIEAM
                     RYNNCDREVLELLPERPVVLVMNKIDRLESKTELLPLIQKISKEFGFAGIVPISAQKG
                     TQLAELLETVRDLLPENPALYPEDAITDRNERFLAAEELREKIFQLSGEEVPYAVSVT
                     VDEFKMERGLRRIQASIIVDKPGQKAILIGAKGEKLKTIATRARKDMEKLFGGKVFLQ
                     VWVKVKSGWAGDAQALKKLGYE"
     gene            13393..14037
                     /gene="recO"
                     /locus_tag="VLL03_03410"
     CDS             13393..14037
                     /gene="recO"
                     /locus_tag="VLL03_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HSS46445.1"
                     /translation="MSAQDKFKQDNQPAFLLHGYPFRETSLVTELFTQNFGRLAVVAR
                     GARRPRSALRGVLQAFQPLLVGWSGKSELRTLHRAEWQGRTPLLQGRSLLCGFYLNEL
                     IMKLLPRDDPHEQLFAHYQHTLLVLAAQNDPATALRRFEIHLLKELGYALTLERDPHS
                     GKSVRPELLYSYVIERGPVASNTELAQCVSWGKPCWTWRTMTTATLILSRRAKR"
     gene            14173..14898
                     /gene="pdxJ"
                     /locus_tag="VLL03_03415"
     CDS             14173..14898
                     /gene="pdxJ"
                     /locus_tag="VLL03_03415"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HSS46446.1"
                     /translation="MIRLGVNIDHVATLRQVRGTPYPDPIEAALLAENAGADTITLHL
                     REDRRHIQDGDVTLLRRRLRVPMNLELAAAREMLQIALRIRPQHACLVPERRLELTTE
                     GGLDVVGHFKRIKHACMLLAEAGVRVSLFINPDCKQLEAAKGTGAPDVEIHTGHYADA
                     SSLGAQNRELKKIRAAVAYGYQLGLQVNAGHGLHYRNVQKIAAIHHINELNIGHAIVA
                     HALFVGWPQAVREMKSIMRKAAR"
     gene            14895..15275
                     /gene="acpS"
                     /locus_tag="VLL03_03420"
     CDS             14895..15275
                     /gene="acpS"
                     /locus_tag="VLL03_03420"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HSS46447.1"
                     /translation="MIYGIGTDLVEVNRIARLLAKYGERFATRVLTRNEREDYASARH
                     AAVFIAKSFAAKEAFAKAVGSGFRYPVILSHISVTHDGLGKPELRFHPALELYLRERN
                     IVRHHLSLSDEKGLACAVVVLERP"
     gene            15290..16171
                     /gene="nagZ"
                     /locus_tag="VLL03_03425"
     CDS             15290..16171
                     /gene="nagZ"
                     /locus_tag="VLL03_03425"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HSS46448.1"
                     /translation="MPLGPLMLDIAGPELAPEDSERLLHPLTGGVILFSRNYQSPQQL
                     LELTSSIHALRNPQLLIAVDHEGGKVQRFREGFTRIPAMRELGKIWDAHSYQAKSLAR
                     MTGFVLAAELRACGVDLSFTPVLDVDHGASTIIGDRAFHSDPQAIAELGHELTLGLKQ
                     SGMGAVGKHFPGHGYIAADSHLEVPVDERSFEDIEMLDLVPFARMISLGLSAVMPAHV
                     IYPKVDLQPAGFSRVWLKDILCKRLGFEGTVFSDDLGMEGASVAGGIIERAQTALHAG
                     CDMVLAWADSWSTPACQ"
BASE COUNT         3629 a         4534 c         4657 g         3351 t
ORIGIN      
        1 acggctgcca tcagccgctc cagtctggtt gatgtggttc gccgtttctc gggctttctc
       61 caccgtgtcc acgaacggca ccgtgatctg cctgaaatca acgccgtcga attgcgtaag
      121 caggctatgg ccgagcattt ccacggtgat gccggtgcga tcggaaatga aaaaagcagt
      181 acgcttgttg gccatggtgg tactctgtgt cgacgagatg caagcagtaa aatatatgat
      241 ttgtgcgctg attacaatta gcaaggtaag ccatggaaca atttatagcc tggtttgaac
      301 ggttgcgcat gagcgacgtg ggaagtgtgg gtggaaaaaa cgcgtcgctc ggggaaatga
      361 tcagccagct ggcaggctcc ggcgtgcgcg tacccggcgg attcgccacc acggccaagg
      421 ccttccgcga gtttctggcg ctaaacgggc ttcagacgcg catcaaaagc agcctgagaa
      481 tgctggatat caacgatgtg gccgtcctcg cggcaaccgg cgctaaaatc cgtcaatgga
      541 ttgcggacgc gccgtttcag ccgaaactgg agaaagaaat ccgcgaggcc taccggaaaa
      601 tgaccgctca ggctaaggcg gatatttcag tggcggtgcg ctcgtcggcc accgcggaag
      661 atttgcccga cgcctccttt gccggccagc aggaaacctt tctcaacatc cgcggcgagg
      721 aaaatgtgct ggccgccatc aagtccgtct ttgcttcgct ttacaacgac cgcgccattt
      781 cctatcgcgc gcaccagggt tttgcccatt ccgaaatcgc gctttccgct ggggtgcaac
      841 gcatggtgag aagcgacttg ggcgcgagcg gggtgatgtt caccctcgat accgaatccg
      901 gtttcgacca ggtggtattc atcacctctt cctacggcct gggcgaaacc gtggtgcaag
      961 gtgcggttaa tcccgacgag ttctacgttt ataaagccgg gttaagacaa aaccgtccgg
     1021 ccatcatccg cagaaacctc ggcgccaagg ccgtcaaaat ggtgcttgcc gagcgccagg
     1081 aagccggaca ctcggtcaaa accctggagg tcattcctca acagcgctcc agtttttcca
     1141 tcagcgatgc cgaagtggag gaactcgccc gctacgcgct gataatcgaa cagcactata
     1201 agcggccgat ggacatcgaa tgggggcggg atggcgtgga tggcaagctt tacatccttc
     1261 aggcgcgccc ggaaacggtg aaatcgcgag ggcgggtcga aattttacga cgctatcgct
     1321 tgaagcaacg ttcagaaatc ctggccagcg gccgcgccat cggccagaaa atcggcagcg
     1381 gcccggtacg gctgattatg gacgcgtcgg aaatgtcgcg cgtgcagccg ggggatgtgc
     1441 tggtcaccga catgaccgac ccggattggg agccggtgat gaaacgcgct tccgccatcg
     1501 tcaccaaccg cggcggtcgc acctgtcacg ccgccatcgt cgcccgcgag ctgggcattc
     1561 ctgcagtcgt cggctgcggc gatgccactc gcaccgtgaa ggaaggcgaa gcggtcaccg
     1621 tatcctgcgc cgaaggcgac acaggcaatg tctacaaagg gatgcttgaa atcgaaatca
     1681 tggatctttc gctcgatgcc ctgcccaagc tgccggtaaa aattaaaatg aatgtgggca
     1741 atcccgagct tgcgtttgat tttcagcgcc tgcccaatgc cggcattggc ctggcgcgcc
     1801 ttgaattcat catcgcccgc atgatcggcg tgcatccccg cgcggcgctg gattatcccg
     1861 atatccctgc ggaattgcgc ggccaagtgg aagagcgcag cgcgggttat cccgatccag
     1921 tgagctatta cgtggaaaaa atcgtggagg gcgtggccac gctggccgct gctttctggc
     1981 ctaagccggt gatcgcgcgc ctgtcggatt tcaaatccaa tgagtattca agcttaatcg
     2041 gcgggcagcg ctacgagccg catgaggaaa acccgatgat cggtttccgc ggcgcgtcgc
     2101 gctacatcgc cgattccttc cgcgactgct tcgcgctgga atgccgggct ttcaaaaggg
     2161 tgcgcaacga tatggggttg accaacgtcg aaatcatggt gccttttgtg cgcactttgg
     2221 atgaggcagc acagaccatc gaattgctca agcaaaacgg gctgcaaagg ggcgtaaacg
     2281 ggctgcgcgt tattatgatg tgcgaggttc catccaacgc gattctcgcc gacgaatttt
     2341 tgcaatactt cgacggattt tccatcggct cgaacgatct tacgcaactt acgctggggc
     2401 tcgatcgtga ttccagccta gtggagggcg gtttcgacga atgcaatcct gcggtgaaaa
     2461 aaatgctgca tctcgccatc caggcctgcc gcagggcggg aaaatacgtg gggatatgcg
     2521 gccaaggccc gtccgatcat ccggatcttg taagatggct gatggaagaa ggcatcgaaa
     2581 gcgtttcgct gaaccccgac tcggtgatcg aaacctggct atatcttgcc ggcgaggcgg
     2641 caaaatagaa tgctcgacat ccggcttaaa ccttccgggt atctgatttt atggcttggc
     2701 gcaactcatg caattgcctt ggggttgttc ctcgttctgt cgctgccggt atggccgaag
     2761 ctggttgcca cgctgttttt ttgtataagt ctggtgtttt gtctcaagcg ccatgcatgg
     2821 cttaccgcac caagttcaat catcgcgctg gaaataaaag aagattgcac ttgcgtaata
     2881 gaaacacgca gtggaaaaag gttagactgt atcctgcttc ctacaagtta tgtatccgcc
     2941 tcgcttacgg tattgaattt caaagccaat ggcgagcgcc tggcgaggca tgccgtgctt
     3001 cttcctgacg cgataaaccc cgaagatttc cgcaaactcc gcattttgct gcgctggaaa
     3061 ttcaaggcaa agccgctcgc gcctattaca taaccggcaa cgcggcggct tccgctctca
     3121 gctgcaaaac ggtattgcgc gcttcgggaa gggctagagg gtaatcgcgg ctgaaatgca
     3181 gcccgcggct ctccttgcgc agccgggcgc ttctcacaat caaatccgcg gtcataacca
     3241 gattgcgcaa ttccagaaga tcgttgctca cccgaaaatt ggcgtagtac tcgttgattt
     3301 cctcatgcaa caaccggatg cggtgctgcg cgcgctccag gcgcttgctg gtgcgcacaa
     3361 tgccgacata atcccacatg aagcggcgca gttcgtccca gttgtgcgaa atcactattt
     3421 cctcgtctgc atcggtcacc cggctctcgt cccatgccgg aagcgcaatg cgaatgcctg
     3481 ccggctgccg cagaatgtcc tgcgccgccg tgcggccgaa cactaggcac tcgagaaggg
     3541 aattgctggc gagccggttc gcgccatgca aaccggtatg cccggtttcg cccaccgcgt
     3601 aaagcccttg aatatcggta tgcgcttgca aatcggtgac aatcccgccg caggtatagt
     3661 gcgccgccgg taccaccggg atcggttgcc gggtgatatc gataccgagc gccagacagc
     3721 gggcgtggat ggtgggaaag tgctcgcgaa caaaatccga tggcttatag gaaatatcca
     3781 gatagacgca atccaggccg cgctttttca tttcgaagtc gatggcgcgc gccacgatat
     3841 cgcgcggcgc gagttccgcg cgctggtcat gctccggcat gaagcgcgtc ccgtcaggca
     3901 aacgcaggag tccgccttcg ccgcgcaccg cctcggaaat caggaacgat ttggcatgag
     3961 gatggtaaag acaggtggga tggaactgga tgaattccat gttcgccacc cggcagccgg
     4021 cgcgccagcc catggcgatg ccgtcgccgg cggcggtgtc ggggttggtg gtgtaaagat
     4081 aaaccttgcc cgcgccgccg gtggccagca cggtgtggtc tgcagcaatg gtttcaacgt
     4141 ggtcggccac gctgtccagc acgtatatgc cgtaacagcg gttatcgggt aacccaagct
     4201 ttctgccggt aatcaaatcc accgcgatat ggtgttccag cacggtgata tcggggtgcg
     4261 ccttgatttt ggcttccagc gtggtttgca ccgcgcgccc ggttgcatcc gcagcgtgga
     4321 tcacccggcg atgactgtgg ccgccttccc gtgtgaggtg ataaccaatt tcactttctt
     4381 cgtcgcgggt gaagggaacg ccctggttaa tcaaccactg tatgctttcg cggccatgtt
     4441 cgaccacgta gcgcgtggcg gcctcatcgc aaagcccggc gccggcgacg agggtgtcct
     4501 ggatatgcgc ggcaatcgaa tcatcatcgc ccaataccgc agcaataccg ccttgtgccc
     4561 agctgctggc gccgtcgatc agcgcttttt tggtaaccag cccgaccttt ttgcggtcgg
     4621 ccaggttgag cgccaccgtc agaccggcta gcccgctgcc aatgatcagt acgtcaaaac
     4681 gccgcaattt ttcgtcccgc gaggctaaaa caatactata gcagagcaag attgggccac
     4741 gcagtctgca tccaccggaa taaccctggt cagaactgaa ctattgcctt aagctgctgt
     4801 ctttagcgct gtacgccgtg tgtgcgtttc ctgagtgcga accgaaaggt tatactagcg
     4861 cgctgccggg gataacttga atgggtagcc gactgcaaca ataacaagcg gaattcaggg
     4921 aagagctgca catgggtgat cgtgaagtcg accagcggct ggtcgaacgc gcacagcacg
     4981 gggacaagca tgcctttgag ttgctggtgg tcaaatacca gcgaaaactg gcgcggttgt
     5041 tgtcccgctt catacgcgat gccgctgaaa tcgaggacgt cacgcaggag gcgtttatca
     5101 aagcctaccg cgctttgccg gcgttccggg gtgacagcgc tttttatacc tggctgtacc
     5161 ggattgggat taacaccgcc aagaactacc tggtagcgat gggacggcgg gcgccgacca
     5221 ccaccgggtt cgacagcgag gaagccgaaa cctttgagga cggcgatcta ctcagggacg
     5281 tcaacacccc tgagaacgag ctgatgagca aggaaattgg caagacagtg aaccagacgc
     5341 tcctggagtt gcctgaggaa ctgaggacgg cgatcacgtt gcgggaaatt gaaggtttga
     5401 gctacgagga cattgcaaca atcatgaatt gccccatcgg cacggtgcgc tcccggattt
     5461 tccgggcgcg cgaagcgatc gccgaaaaac tacggccgtt gctgggcacc agcaaggaca
     5521 agaggtggtg accatgaaac aagacatttc gggattgatg gatggcgagc tggacccaga
     5581 tgccgccacg gcggtgattg ccaggcttaa gcatgaggaa gaccttaaaa acgcctgggc
     5641 cgactatcac cttatcggtg acgccctacg tcaggacgct accctgtttc tcggcttcag
     5701 cgagcgcctg agcgaaaaac tagggcatga gcccgccata ctggcgccgc gccggacgtt
     5761 ggcgcagaaa gtcaaattca ccgccctgtc tgtcgccgct tcgcttgctg cggtagccat
     5821 ggtcggatgg gtggtgcttc agaactacac ggacaagccc aaggaaaacc tcgccgccac
     5881 gcaaccaacc agcgcacaag ccatgctcgc cagcctgccg cgatatccct tcaacagcaa
     5941 tgtcaacgaa tatcttcttg cccatcagga attttctccc agcactacca tgcaaggcgt
     6001 ggtttcttac gccaggacgg tatccggcga aggcagggaa gcgagccgat gaaagcggta
     6061 ttgtttgcct tgctgctctg gcccgcggcg ctgcttgcgg acacagcacc tgacgaggcg
     6121 ctcgcctggc tgcaaaaaat cgcttccgcc gcacgggaac tcaattacaa cggcaccttc
     6181 gtttaccaat acggcaacca cgttgaaacc tcgcgcatcg tacacgccgt ggacggctcc
     6241 ggggagcagg aaaaactgga gatcctcgat ggcctgccgc gtgaaatcat tcgcagcaac
     6301 gatgaagtgc gttgttattt gccagagagc aaaacggtga tggtggaaaa acgcacccac
     6361 cgaacaaaat ctttcccggc gctgctcccc gagcagctca acaacctgaa cgaaagttac
     6421 cttatcaaaa tgggcgggca tgagcggatt gccggatttg actgccaggc gctgatactg
     6481 gaacccaggg acggcttgcg ctacggccac aagttttggg ccgaaatgaa ttccgggctg
     6541 ctgctcaaag ccagcatgct ggatgaaaat aacgaagtcg tcgagcaatt tgcctttacc
     6601 cagctttcca ttggcgggcc gatcgacaag gaaatgctca aaccccgatt ttcggccaaa
     6661 accccggaat ggcgttacga ccacgcggcg caggccgagc cgttttccac gtcaaccggc
     6721 tgggccatca ggcccccgcc ggccggcttc aagaaaatca tggaaacccg gcgcgtaatc
     6781 agcggcaagt cggcgccggt ttcgcatatc gtctattccg atggcctggc tgcggtgtcg
     6841 gtatttatcg aaccactggg ccccaagaca aaatccatga acggcctgca aaagcgcgaa
     6901 gcgcttaatg tctacaccaa accggttgct gacttccaga ttacggtgct gggagagacg
     6961 ccgccgatta ccgtgatgga gatcggcaac tccgtttact attccgggaa ataaaaaccc
     7021 cctttagcca ccgcttctct cgctctttag ctaatccttt aattacggtt tgagccatca
     7081 cattttcaag tgaaacaaag gaagtaaatc aatgaaaaaa ctgctttggg catttgtctt
     7141 aatgttttcg tttgccgttt cgggttttgc caaagaactg ccggatttta ccgatctggc
     7201 ggaaaagcag gggctggcgg tggtgaacgt cagcaccatg cagaccgtgc gcagccaggg
     7261 cgtcccgcaa cttccgtttt ccgaggatga ccctttctat gaattcttcc gccgctttgc
     7321 gccgcccatg ccgcgcgaat tcgagtccag gtcgctgggc tcggggttca tcatcagcgc
     7381 ggacgggtac gttctcacca atgcccatgt ggtggacagc gccgacgaga ttaccgtcaa
     7441 gcttaccgac aagcgcgagt tcaaggccaa ggtcatcggc agcgacaaac gcaccgacgt
     7501 ggcgctcatc aagattgacg ccgccagctt gcccaaagtt acattgggcg atcccaacaa
     7561 actgaaagta ggcgaatggg tggtcgccat cggttcgccg ttcggctttg aaaacagcgt
     7621 gaccgccggc atcgtcagcg ccaaaggacg ttcgctgccg caggaaaatt tcgttccttt
     7681 cattcaaacc gatgtggcga tcaatccggg caattcaggc ggaccgctgt tcaaccttaa
     7741 aggcgaggta gtcggaatca attcgcagat ttacagccgc accggaggat tcatgggact
     7801 ttcgttcgcg attccgattg acgtggcgat ggaagtcgcg gatcagctgc gtaccaccgg
     7861 caaagtcagc cgcggccgca tcggcgtggt tatccaggaa gtgactaagg agcttgccga
     7921 gtcgttcggc ctttcgaaac cgacgggagc attgattaat tcggtggaaa aaggcggtcc
     7981 cgccgacaag gccggtcttg agccgagcga cgttatcctt aagttcgatg gcaagtcggt
     8041 gagtaactct gcggatttgc cgcgcatcgt cggcgccact aaaccgggct cgaaagtcac
     8101 cgtgcaggtc tggcacaagg gtgcggctaa ggacattaac cttacggtcg gcgaaatctc
     8161 ctccgacgac aagacggcgc agcgccaggg caagtatggc aaagagccgg atacccgtta
     8221 tggcatgctg ttggacaacc tgactcccga ccagagaagc gagcttggca tcaaaggcgg
     8281 cgtgctggtg gaagacgtgc agggtgcggc cgcgcgcgcc ggcatccgtc gcggcgacat
     8341 catccttgct gtaaacaacc aggatgtgaa gtcggtcgag cacttcaatc agttgttgag
     8401 caaatatgaa aaagggcgca gcgtggcctt tttgatccgg cggggcgaca atgcgcttta
     8461 catcccgctg cgcatcaatg gcagctgaaa gccggcgcaa gctgacggta ctctccaggg
     8521 aatactgcca tctatgcgaa gacatggtcg ccgcgctgcg ccagctgcag gctcaacgcg
     8581 gctatttcga tcttgaagtc gttgatgttg actgtgatgc cgcgctcgaa gagcgctacg
     8641 gcgagcgggt tccggtgctg cttctggacg gcgtggaagt ctgccattac cgcttcgacc
     8701 cggaggtgct ggatgcgcat ttaatagcag tcacacgcca agcctaaaat ccgttagaat
     8761 ttcacatggt ttacataagg caagggcacg atggtgccct tttttttgat gcatgaatct
     8821 tatccgcaat ttttccatca ttgcgcacat cgaccacggg aaatccacgc tggcggaccg
     8881 ctttattcaa atctgcggtg gtttgagcga gcgtgaaatg gaagaacagg tgctggattc
     8941 gatggacctc gagcgcgaac gcggcattac catcaaggca caaaccgccg ctctcaatta
     9001 caaggcgcgc gacggtcagg actatcttct caatctgatc gacacaccgg gccacgtgga
     9061 tttttcctat gaagtgtcgc gttcgctcgc cgcttgcgaa ggcgcgctgc tggtggtgga
     9121 cgtctcacag ggcgtggagg cgcaaaccgt ggccaactgc tatacggcat tggagcaggg
     9181 cgtggaagtg gttccggtgt taaacaagat ggatttgcct tccgccgacc ccgagcgcgt
     9241 catcgccgaa atcgaggaca tcatcggcat tcccgccaag ggagcgctgc gcgtcagcgc
     9301 caaaaccgga ctgggagtgg aagaaattct cgaatcggtt atccggcgca tcccgccgcc
     9361 caaaggtaaa ttggacgccc cactcaaggc gctgattatc gattcctggt ttgacaatta
     9421 tgtcggagtg gtcatgctgg tgcgggtggt ggacggcact ctaaagccaa agaacaaggt
     9481 tcttttaatg tcaaaccagg cgacttatct ttgcgaggag gtcggggtgt tcacgccgaa
     9541 accgaaaacc aaggattcac tatcggcggg cgaggtcgga tttgtgattg caggcatcaa
     9601 ggagctcagt gccgccaagg tgggtgacac cctcacgctg gccgtcagcc cggccccggc
     9661 cccgctgcct ggcttcaagg aaatcaaacc ccaggtgttc gccggcctgt acccggtgga
     9721 ttccaaccag tacgacgcgc tgcgcgacgc gctggaaaaa ctcaagctca acgacgcctc
     9781 gctgcactac gaaccggaaa cgtctcaggc cctcggtttc ggtttccgtt gcgggtttct
     9841 cggcttactg cacctggaaa tcgtacagga gcggctggag cgcgaatacg gcatgaacct
     9901 gattaccact gcacccaccg tggtgtatca ggtattgctg cgcaacgggg aggtgctgga
     9961 aattgaaaac ccgtccaaaa tgcccgaccc gtctaaaatc gaggaagtcc gcgagcccat
    10021 catcacggcc accatcctgg tcccgcagga ttacgtggga ccggtgatta ccttgtgcat
    10081 cgagaaacgc ggcgtgcaga aaaacatgca atacctgggt cgtcaagtat tgctcaccta
    10141 cgagctgcca ctgaatgaag tggtgatgga ttttttcgac agactgaaat cggtcagccg
    10201 cggttatgcg tcgctcgatt acgaattcga ggcgtatcgc gccgccgatt tggtcaaact
    10261 cgatgttctg attaacggag acagggtgga tgcgctctcg ctgatcatgc accgtgccaa
    10321 cagccagtac cgcgggcgcg aactggcggt aaaaatgcgc gaactcattc cgcggcaaat
    10381 gttcgacgtc gcggtgcaag ctgccatcgg ctcacacatt atcgcgcggg aaaccatcaa
    10441 ggcgatgcgt aagaacgtgc tggcgaaatg ctacggcggt gacatcacgc gcaaaaagaa
    10501 actgctggaa aaacaaaaag ccggcaaaaa acgcatgaag caggtcggca acgtggaaat
    10561 cccgcaggag gcttttctcg caattttgcg ggtggaaagt aaataaccca tgaatttcgc
    10621 cctggtcatg ttcattttac tgataggcac cggcggcatt acactgctcg atcgtctctg
    10681 gctgaaaagc cggcgccggc ccgatcgcac cgaaccctgg tgggtggaat accccaagag
    10741 tttttttccg gtgattttgg tggttttttt actgcgctcc tttctggttg aaccgttcaa
    10801 gataccttcc ggctcgatga tcccgaccct gctggtgggg gatttcattc tggtcaacaa
    10861 attcacctat ggcatccggc tgcccatcct caacgccaag atcattgaca tcgacgaacc
    10921 caggcgtggt gaagtcatgg tgttccgcta cccggaaaac ccgtcactgg attacatcaa
    10981 gcgagtagtg ggcctgcccg gcgacagggt ggtctaccgc aataagcgtt tatggatcaa
    11041 cggagaggag atcaagatta cgccttccgg cgagtataat tatgtcgagt ccgggctgaa
    11101 cttcattacc gcgaaacgtt ttaccgagaa cctgggcggg catagacata ccatactcgt
    11161 ccagaaagaa gcgccggcgg tgcagacggg aggcgtgcgg ccattcccgt ttcgcgaaaa
    11221 ttgcgtctac aatgaagaag gctttgcctg cacggttccg gcaggccatt atctcatgct
    11281 cggcgacaac cgagacagca gcagcgacag ccgttattgg ggttttgtgc cgcagggaaa
    11341 catcgtcggc aaggcattta tgatatggtg gaattttgat aacttgaagc gcatcggcca
    11401 atccatcgaa tagggggtgg tatgaaacga caacaaggcc ttactatgac aggttttttg
    11461 ttcctggcga tcgctttggt gtttgcagcg ctgcttggtt tcaaagtgct gcccgcctac
    11521 ctggaatact tttcgataca gcgagctctc agtggcatgg cgcatgacct ggatttgcaa
    11581 actgcaacac ccaaggagat ccggagctcg ttcgacaatc gcgcagcaat tgataacatc
    11641 accaccatta ccggtcaaga cctagaaatc gataaggaag gcaatcgagc cgtggtacgt
    11701 gccatctatg cgcaaaagat ccccctgttc gccaatatca gcgcctacat tgagtttcaa
    11761 gcctcaacag acaaacgcaa gtaggaggct ataaccggtc ataatggata tcgaaagcgt
    11821 ttgccgtaaa cttggctatt cgtttcgtca aaccgagctt ctgcgtcagt ccctgactca
    11881 tcgcagcttc ggttcgcctc acaacgaacg tttagagttt ctgggcgaca gtatattaaa
    11941 ttgcgttatt gcgcacgccc tgttccggcg ttttccggct ttaagcgaag gcgagctttc
    12001 gaggctgcgc gccaatctgg tcaaccagca aagcctgttc gagctcgcgc aaaagctgaa
    12061 cctgggcgat gaagtgcttc tcggtgaagg cgagctcaaa agcggtggct tccgccgccc
    12121 ctccattctg gccgatacgc tcgaagccat gatcgccgcg gtcttcctcg atggcgggtt
    12181 cgaccaggcc gggcaggtgg tccaaaacct gtacgccacg ttgttgcagg acatcgatcc
    12241 taaaaatccc ggtaaggacc ccaaaaccct gcttcaggaa tttctgcaaa gccgcaaaat
    12301 ggcgttgccc caatacagcg tggtcgccac cgcaggcgaa gcccatcaac agcaattccg
    12361 agtggaatgc ttggttgcgg agcttaacat ccgttctttc gggaccggca ccagtcggcg
    12421 caatgccgaa caggatgcag caaggcaggc ctatcagctt gccgccggtg cctgactcat
    12481 gccgggctca atccataacc gctatcgctc cgggctgatt gccgtggtcg gacgccccaa
    12541 cgtcggcaaa tccactttgc ttaaccggct catcgggcaa aaaatcagca tcacctcgcg
    12601 caaaccccaa accacccggc atcgcatcac cggcatcctc actcgcgaag atgcgcaata
    12661 tatctttgtc gacacacccg gtttccagac ccggcacggc ggcgcgctca accggattct
    12721 gaaccgcagc gtcgtacaaa gcctggatgc ggcggatgcg gtgttgtttg tgatcgaggc
    12781 catgaggtac aacaactgcg accgggaagt gctggagctc ttgcccgaac gcccggtggt
    12841 gctggtcatg aataaaatcg accgccttga aagcaaaacc gaactgctgc cgcttatcca
    12901 aaaaatttcg aaagaatttg gctttgctgg cattgttcct atcagcgcgc agaaaggtac
    12961 gcaacttgca gaacttcttg aaaccgttcg tgacttgctg ccggagaacc cggcgcttta
    13021 tccggaagac gccatcaccg accgcaatga gcgcttcctg gcggcggaag agctgcgcga
    13081 gaaaattttc cagctgtccg gagaagaagt gccttacgcc gtcagcgtaa ccgtggatga
    13141 attcaagatg gagcgcggat tgcgccgcat ccaggcttcg atcatcgtcg acaaaccggg
    13201 gcaaaaagcc atcctgatcg gggccaaagg agaaaaactc aagaccatag ccacgcgggc
    13261 gcgcaaagac atggaaaagc tttttggcgg caaggtattt ttacaggtat gggtcaaagt
    13321 gaaaagcggc tgggccggtg atgcgcaagc gctgaaaaaa ctgggctacg aataggcatt
    13381 accggggaat ccatgagcgc gcaggacaag tttaaacaag acaatcagcc tgcatttctg
    13441 ctgcacggct acccgttccg tgaaaccagc ttggtaaccg aattattcac gcaaaacttc
    13501 ggccggctgg cggtagtcgc acgcggtgcg cgccggccga gatccgcgtt gcgaggagtg
    13561 ctgcaggctt tccagccgct gctggtcggc tggtccggaa aatcggaatt gcgcacgctg
    13621 caccgcgcgg aatggcaggg gagaacacct ctgcttcaag gcaggagctt gctttgcggt
    13681 ttttatttga acgagctgat aatgaagctt ttgccgcgcg acgatcctca cgaacagctg
    13741 ttcgcgcatt accagcacac gctgcttgtt ctcgctgcac aaaacgaccc cgctacggcc
    13801 ttgcgccgct ttgaaatcca tctgctcaag gaactgggtt atgcgttgac gctggagcgc
    13861 gacccgcata gtggcaaatc ggtgcggcct gaacttcttt actcctacgt catcgaacgc
    13921 ggcccggtcg cctcaaacac cgagctggcg caatgcgtat cctggggaaa accttgttgg
    13981 acttggcgaa cgatgactac agcgacccta atactcagca ggagagcaaa gcgctgatgc
    14041 ggcttattct cagccattat ctcggtaacc aggtgcttta cacccgtcag ctgctcaagg
    14101 atttacaaca gctttaaacg cctggccgcg gttattgcgg atataatttg tccttttaat
    14161 caaatcagaa acatgatacg gctgggagta aacatcgatc acgtggcgac gctgcgccag
    14221 gtgcgcggca cgccttatcc cgatccgata gaggccgcac tgctggcgga aaatgcaggt
    14281 gccgacacga ttaccctgca tttgcgcgaa gaccgccggc atattcagga cggcgatgta
    14341 accctgctgc gccgcaggtt acgcgtgcct atgaacctcg aactcgccgc tgcccgagag
    14401 atgttgcaga ttgcgctgcg catacgcccc cagcacgcct gcctggtgcc ggaacggcgg
    14461 ctggaactta ccaccgaagg cggattagac gtggtcgggc atttcaagcg catcaagcac
    14521 gcctgcatgc tgctcgcgga agccggagtg cgcgtttcgc ttttcatcaa cccggattgc
    14581 aaacaactgg aagccgcgaa ggggaccggc gcgccagatg tggaaattca caccggccat
    14641 tatgcggatg cgtccagcct cggtgcgcag aacagggagt tgaaaaaaat ccgcgcggcg
    14701 gtggcctatg ggtaccagct gggcctgcaa gtcaacgccg gccacggtct tcattatcgc
    14761 aatgtgcaga aaatcgccgc cattcaccac atcaatgagc tcaatatcgg ccatgccatc
    14821 gttgcacatg cattgtttgt cggctggccg caggcggtgc gggaaatgaa aagcatcatg
    14881 cggaaggcgg cgcgatgatt tacggcatag gcaccgacct tgtcgaagtc aaccggattg
    14941 cccgactgct ggcaaaatac ggcgagcgtt tcgccacaag agtgctgact cgcaacgagc
    15001 gggaggacta tgcttctgcg cgtcatgccg ccgtattcat cgccaaaagt tttgccgcga
    15061 aagaggcgtt tgccaaggca gtgggcagcg gttttcgcta tccggtgatc ttaagccata
    15121 tcagcgtgac acatgatggc ctgggcaaac ctgaattgcg gttccatcct gcgctcgaac
    15181 tttatttgcg ggagcgcaac atcgtgcgcc atcacctgag cctgagcgac gagaaaggcc
    15241 tggcttgcgc ggtggtggtg ctggaacgcc cgtgaaaagc gcaagatcca tgccgttggg
    15301 gccgctgatg ctcgatatcg ccggaccgga gcttgcgcct gaagacagcg agcggctgct
    15361 gcaccccctg accggcggcg tcattctctt ttcccgcaac taccagtcac cgcagcaact
    15421 gctcgaactc acctcgtcaa tccatgcgct gcgcaacccg caactcctca tcgcggtgga
    15481 ccatgaaggg ggcaaggtgc agcgtttccg cgaaggcttt acccgcattc cggcgatgcg
    15541 cgagttgggg aaaatctggg acgcccattc gtatcaggcg aagagccttg cgcgcatgac
    15601 tgggtttgtt ctcgccgcgg aacttcgcgc ctgcggcgtg gatttgagct ttaccccggt
    15661 gctcgacgtg gatcatggcg cgagcaccat tatcggcgac cgcgctttcc acagcgatcc
    15721 gcaagcgata gccgagctcg ggcacgagtt gacactgggc ttgaaacaaa gcggcatggg
    15781 cgctgtaggc aagcattttc ccggccacgg ctatattgcc gccgattcgc atcttgaagt
    15841 gccggtggat gagcgcagct ttgaggacat cgagatgctg gacctcgtgc cttttgcccg
    15901 aatgataagc ctgggcttga gcgcggtgat gccggcgcat gttatttatc ccaaggtaga
    15961 cctgcagccg gcgggcttct cccgagtctg gctcaaggac atcctgtgca aaaggcttgg
    16021 gtttgaaggc acggtcttca gcgatgattt gggcatggaa ggtgccagtg tcgccggcgg
    16081 catcattgag cgcgcgcaaa cggccttgca tgccggctgc gacatggtgt tggcatgggc
    16141 cgattcgtgg agtactccgg catgtcagta g
//
LOCUS       DATIOW010000105         3129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1878938, whole genome shotgun sequence.
ACCESSION   DATIOW010000105 DATIOW010000000
VERSION     DATIOW010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1878938"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VLL03_03430"
     CDS             complement(<1..321)
                     /locus_tag="VLL03_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46449.1"
                     /translation="MQKKFSILGLLLLTTGAFAAQDDQDDAQPQAEQPAVEAPVEAQP
                     ALPPLDTAKLGKVRSAVVLVVGQEQEQPLYAKNVDKQMPIASITKLMTAMVVLDAQLP
                     LDEPV"
     gene            complement(670..2499)
                     /locus_tag="VLL03_03435"
     CDS             complement(670..2499)
                     /locus_tag="VLL03_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HSS46450.1"
                     /translation="MRIAHFGKPPQNRNDWKTIGTLLPYLLEFKGRVVLALSLLICAK
                     LANVTVPLVLKGIVDSLSTKDALIALPIALLAGYGILRFSATLFGELRDAVFAKVTQR
                     AIRRAALQVFSHLHLLSLRFHLERQTGGVSRDIERGSRGISFLLQFMLFNILPTFVEI
                     GLVAAILLIQYDVWFAVVTFVTLAIYIVFTLAVTEWRMVFRRTMNDSDSKANTRAIDS
                     LLNYETVKYFGNEEWEARRYDEGMQVWENAAVRNQTSLAGLNAGQSAIIAVGMTLLMV
                     LAANGVVKGAMTIGDLVLVNAFMLQLYLPLNFLGFVYREIRHSLADMERMFSLLEVNL
                     EIQDKPDAVPLMVGDAAVHFERVNFSYEAKRQILFDVDFEIAPGETVAVVGHSGSGKS
                     TLARLLFRFYDVNGGRIRVNGQDIREATQKSLRAAIGIVPQDTVLFNDTIFYNINYGR
                     PEADREEVIEAAKSAHIHDFIATLPDGYDSMVGERGLKLSGGEKQRVAIARAILKNPR
                     ILIFDEATSALDSKSEKAIQAELKRIAANRTTLTIAHRLSTVVDADQILVMDHGHIIE
                     RGTHRELLEKQGAYAHMWALQQQEEAKRDKEEQSPHRAEAVRA"
     gene            complement(2619..>3129)
                     /locus_tag="VLL03_03440"
     CDS             complement(2619..>3129)
                     /locus_tag="VLL03_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HSS46451.1"
                     /translation="QEILIASAYFLPGRNLRRALVSAAQQGVRVILLLQARVEYVLLH
                     YASRALYGNFLDAGVEIYEYHKSFMHAKVAVVDGGWATVGSSNIDPFSLLLAREANVV
                     VQDRKFAGELRKNLMQAVAEGAMQVVHERWEHQPLWRRILTWISYGVVRFIAGVFGYG
                     REQELKPRH"
BASE COUNT          663 a          956 c          853 g          657 t
ORIGIN      
        1 gaccggttca tccagcggca attgtgcatc gagcaccacc atcgccgtca tcagcttggt
       61 aatggaagca atcggcattt gcttatcgac gttcttggca tagagcggct gctcttgttc
      121 ctggccgacc accaagacga ccgctgaacg caccttgccc agcttcgccg tgtcgagcgg
      181 cggcaaggcc ggttgcgcct caaccggcgc ctcaaccgct ggttgctccg cttgcggttg
      241 agcatcgtcc tgatcgtcct gggcggcaaa cgctcccgtt gtcaacaaca gcaagcctag
      301 aatagaaaat tttttctgca ttggttttgt atcttaagtt attgaaacga aacggctccc
      361 cctgtccttc cctgaaagcc taggctcatg atcgcagcta aaacaacaaa aatcaaggcc
      421 taagttcaca tttttaacca caatttttaa gggtgacacc gacctcattg aattcatgat
      481 ttgattaagg cctaaaaagt aaggcctgga ctgcatccct ggcggtttcc cttcttcggt
      541 aaagcgcgag acagcagcga caacccggcg cagaggcgaa gccgccggca atcggcagcg
      601 ccggtagtgc acagtcgagc atgttttcta tgaatttgaa cccggacagt atgggggtac
      661 tggcttagtt caggcgcgca ccgcttccgc ccggtgtggg ctttgttctt ccttgtcgcg
      721 cttggcttcc tcctgctgct gcagcgccca catgtgcgcg taggcaccct gcttttccaa
      781 aagctcgcga tgggtgccgc gctcgatgat gtggccgtgg tccatcacca gaatctggtc
      841 ggcatcaacg acggtggaaa gccggtgggc gatggtaagt gtggtccgat tggcggcgat
      901 gcgtttgagt tccgcctgaa tcgctttttc cgatttggaa tcgagcgccg aagtcgcctc
      961 gtcgaaaatc aggatgcgcg ggttcttgag tatcgcgcgc gcaatcgcca cgcgctgctt
     1021 ctcgccgccg gaaagcttga gtccccgctc gcccaccatg ctgtcgtaac catccggcag
     1081 cgtcgcgatg aaatcatgaa tgtgcgccga tttcgccgcc tcgatcactt cttcgcggtc
     1141 cgcctcgggg cggccgtaat tgatgttgta aaaaatcgtg tcgttaaaca gcaccgtgtc
     1201 ctgcggcacg atgccgatgg ctgcacgcag ggatttttgt gtggcctcgc ggatgtcctg
     1261 gccgtttacg cggatgcgcc cgccgttcac atcgtaaaaa cgaaacaaca gccgcgccag
     1321 cgtggatttg ccggaaccgc tgtgcccaac caccgccacc gtttcccccg gcgcaatttc
     1381 aaaatccacg tcgaacaaaa tctgccgctt tgcttcgtag ctgaaattca cgcgttcaaa
     1441 atgcacagcg gcgtcaccca ccatcagcgg cacggcgtcc ggcttatcct ggatttccag
     1501 attaacttcc aaaaggctga acatcctttc catgtccgcc agcgaatggc ggatttcgcg
     1561 gtagacgaag cccaggaagt tgagcggcag atagagctgc agcatgaagg cgttcaccag
     1621 caccagatcg cctattgtca tcgcgccttt caccacgccg ttcgctgcaa gcaccatgag
     1681 cagcgtcatg ccgacggcga tgatcgccga ctgcccggcg ttgagcccgg cgagcgaggt
     1741 ctggttcctc accgccgcgt tctcccacac ctgcatgcct tcgtcatagc gccgtgcttc
     1801 ccactcttcg ttgccgaaat atttcaccgt ctcgtaattc agcaggctgt cgatggcgcg
     1861 ggtgttggcc ttggagtcgg aatcgttcat ggtgcggcgg aacaccatgc gccactcggt
     1921 gaccgccagc gtaaatacga tgtaaatcgc gagcgtgaca aaggtcacca ccgcaaacca
     1981 gacgtcgtat tggatgagca gaatggccgc gaccagccct atttccacga aggtcggcag
     2041 gatgttgaac agcatgaact gcaaaaggaa ggaaatgccg cggctgccgc gctcgatgtc
     2101 gcgcgatacc ccgccggtct gccgctccag atgaaagcgc aagctgagca aatgcaaatg
     2161 gctgaatact tgcagcgccg cgcggcgaat cgcccgttgc gtaacctttg cgaataccgc
     2221 gtcgcgcaat tcaccgaaca gcgtcgccga aaagcgcaaa attccgtaac cggcgagcag
     2281 cgcaatcggc agcgcaatca gcgcgtcttt ggtgctcaag gaatcgacga tgcctttgag
     2341 caccagcggc acggtaacgt tggcgagctt ggcacaaatc agaagtgata gcgccagcac
     2401 cactcttccc ttgaactcca aaagataggg aagcagcgtc ccgatggtct tccagtcgtt
     2461 gcggttttgc ggcggcttgc cgaaatgagc gatacgcata gctggtttct caaaagcttt
     2521 agcgtatcac cctgcgccgt gattggcgag tggattcaaa gacctggaaa ccgtcaccgc
     2581 tccgacacag cgcgaaggcc tccgctgttc gcagccgtct aatggcgcgg cttcagctcc
     2641 tgctccctgc cgtagccaaa cacgccggcg atgaagcgca ccaccccgta gctgatccag
     2701 gtcaggatac gccgccatag cggctggtgt tcccagcgct catgcaccac ttgcatcgca
     2761 ccctcggcca ccgcctgcat cagattcttt ctcaattccc cggcaaattt ccggtcctgc
     2821 accacgacgt tggcttcgcg cgcaagcaac aggctgaagg gatcgatgtt ggaggatccg
     2881 accgtcgccc aaccgccatc caccaccgcc accttggcgt gcataaagct cttgtggtat
     2941 tcgtagattt ccacacccgc gtcaagaaaa ttgccgtaga gggcgcgtga agcgtaatgc
     3001 agcaatacat attcgacccg cgcctgcaac agcaaaatca cgcgcacgcc ttgttgcgcc
     3061 gcgctcacca gcgcgcggcg caaattgcgg ccgggcagga aataggcgct ggcgatgagg
     3121 atttcctgt
//
LOCUS       DATIOW010000106         7948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1677029, whole genome shotgun sequence.
ACCESSION   DATIOW010000106 DATIOW010000000
VERSION     DATIOW010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7948
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677029"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VLL03_03445"
     CDS             complement(<1..626)
                     /locus_tag="VLL03_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSS46452.1"
                     /translation="MSLAQLRGQLQRLETKGLLRHRRIVQSQQGAHVRADGRELVSFC
                     SNDYLGLASHPELIKAAREGAELYGVGAGASHLVSGHFDAHHQVEEKLAQFTGLPQAL
                     LFSTGYMANLGVVTALLGRDDALFADKLNHASLNDAALLSRAKFHRYPHLDMRALGKR
                     LTASGTKRKLVMTDAVFSMDGDIAPLPDLLALCERFDTWLLIDDVHGFG"
     gene            complement(672..1634)
                     /gene="bioB"
                     /locus_tag="VLL03_03450"
     CDS             complement(672..1634)
                     /gene="bioB"
                     /locus_tag="VLL03_03450"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HSS46453.1"
                     /translation="MKPATQIAEIRQVWRVDEVEALFELPFNDLVYRAQQIHRQYFDA
                     NAVQLSTLLSIKTGGCPEDCAYCPQAARYHTGVADEDILPVAGVIAAARAAKDNGATR
                     FCMGAAWRGPKQKDLAKVIEMIKAVRALGLETCATLGLLKEGQAEQLREAGLDYYNHN
                     LDTAPDFYGKIITTREYRDRLDTLSRVRGAGIHVCCGGIVGMGETRRSRAVLIAQLAS
                     LDPYPESVPINNLVQVEGTPLYGAEPLDPLEFVRTIACARITMPKAMVRLSAGRSQMP
                     EAMQALCFLAGANSIFYGGKLLTTGNPEMRQDQALLDKLGMHPM"
     gene            1770..2426
                     /locus_tag="VLL03_03455"
     CDS             1770..2426
                     /locus_tag="VLL03_03455"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HSS46454.1"
                     /translation="MQAECLLCGAAVANQDLCQACLEHLPYLAPACCKVCAVPLSQGE
                     ICGACLSKPPAFDRTYAAFSYSFPVDALIQALKYRGNFAVTPLLASALEPIARSASPV
                     DCLIPVPLSAARLRKRGFNQALEIARPVARAIGVALLATGCSRVLDCAPQAGLPWKER
                     VKNIRGAFACEADLSGKKVALIDDVMTTGATLDELAKVVRRKGAVEISVWVAARTLPP
                     "
     gene            complement(2491..3531)
                     /locus_tag="VLL03_03460"
     CDS             complement(2491..3531)
                     /locus_tag="VLL03_03460"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS46455.1"
                     /translation="MSIINQMLLDLEKRHVPRLDGVLSQARALPPTRSSMSTRWAVLL
                     VLLILVVGVFAWQLLREEEPTPAIANASVPVPAAPPAKLATGEKTVAEDLVVAEPLFK
                     LASRLSLELNNPPPEPEQLVPDKPAAKPANEPPVKAKSKPTPSSVAAINKQMRQETPQ
                     QQAENEYRKAAAHLQQGKIQEAEESFRQALRLNSNYAAARLALAGLLLQEKRANDAEN
                     LLREGLDQESGQAGFAMILARIQVEKGETQAALETLQKTLPHAAQNADYRAFLAALLQ
                     RESRHSQAVEQYQAALQLKPAGVWYMGLGISLQALQRLPEARDAFQQAMISGALSPEL
                     QAYVEQRLRQVK"
     gene            complement(3528..4421)
                     /locus_tag="VLL03_03465"
     CDS             complement(3528..4421)
                     /locus_tag="VLL03_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSS46456.1"
                     /translation="MYRAHFGLREPPFGLTPDTSFVFACTAHQQALNTLLVAAKSGEG
                     FMKITGEVGAGKTLLCRRFLASLGESFVSAYILNPYLDPRSLLLALADELRTPLQKDA
                     DMHQLLKSLTLALLRFARQGKQVVICLDEAQAMPQESLEALRLLTNLETEKRKLVQVV
                     LFGQPELDEKLAHPSVRQLRQRITFHCRLDGLDRSELDHYLVHRLSVAGYHGSRIFTR
                     RAVRLMHRLSGGIPRLVNILAHKSLLLTYGEGGHEVVPRHVRIAATDTPAAKNGIEPW
                     WWLAFAMLLVSLSGIGWTFLQ"
     gene            complement(4487..6205)
                     /gene="mshL"
                     /locus_tag="VLL03_03470"
     CDS             complement(4487..6205)
                     /gene="mshL"
                     /locus_tag="VLL03_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus (MSHA type) biogenesis protein MshL"
                     /protein_id="HSS46457.1"
                     /translation="MARYDTTLDQINAELDKAVEKRPEPAQPEAVSRALLPPLTVELP
                     KPEGKAAETRFDLVVNNAPANQVFLAVVSGTPYSMLVHPGVREPISLNLKDVTVFDAL
                     DAIRELYGYEYKVDGTRIFIQSLTLQTRVFQVNYLEGKRKGSSDIRVTSSGAITTGAG
                     GVAVTPIVTTTGAGGGGVGVESSKITTTSTTDFWEELTKALTAIIGKAEGRHVVVSPQ
                     SGVVVVRAMPAELRNVAAYLKAAQVSVERQVILEAKILEVQLNDGFQSGINWAAFRNT
                     NNSRGSVGQLTPGTTLSTTGTLTGGTTATPTGGLSAGTLGNVTIAGTPIISAPGTDIS
                     SAAGAAGALFGLAFQTSNFAALISFLETQGNVHVLSSPRIATLNNQKAVLKVGQDELF
                     VTNVSTTTTTGTATTTTPTVTLQSFFSGIALDVTPRIDEDNNIILHIHPSVSDVRQVQ
                     RNITLGGTLGSISIPVPRSAIQETDSVIRARDGQIVAIGGLITQSQTTDRSQIPGLGD
                     LPVAGNLFRENNKVSQKRELVILLKPTVIHNDTEWQEDILQARERVQSLRRDSSSSKT
                     DDSAGK"
     gene            complement(6264..6587)
                     /locus_tag="VLL03_03475"
     CDS             complement(6264..6587)
                     /locus_tag="VLL03_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSHA biogenesis protein MshK"
                     /protein_id="HSS46458.1"
                     /translation="MPDPTRPPSELEASVVSPNGAQTGPVLQSVLISPVYKVAIISGE
                     IVTLGGQYGKARVVKISESEVVLNTGGNLQTLRLFPDVEKKTGYSGAVTEKKTRRGGV
                     SHGEE"
     gene            complement(6758..7402)
                     /gene="gspM"
                     /locus_tag="VLL03_03480"
     CDS             complement(6758..7402)
                     /gene="gspM"
                     /locus_tag="VLL03_03480"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HSS46459.1"
                     /translation="MKYDWKTLAGKIDDMNLRERALIFGMLALILVALVFTVFLDPLF
                     ARQKILSQQIVQKQNQIKSIQAQIQAIVEPGSDPDAANRAKQEALKQQLAQSEAYLQG
                     KQQHLIPPDKIAALLEEILNRNRRLQLVSLQTLQASTLSGKEGRDEKEMFKHGVEITV
                     RGGYLDLLEYVTELEMLPSQMFWAKVSLSVETYPSSRLSLTLYTLSLDRIWMRV"
     gene            complement(7399..>7948)
                     /locus_tag="VLL03_03485"
     CDS             complement(7399..>7948)
                     /locus_tag="VLL03_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46460.1"
                     /translation="ALSVQAQETEKKLAAEQARLEKFAAQYAPLKKSTFLEQEIGRLE
                     AQVKARQEIVETLKSGALGSTNGYSRYMRAFARQSVSGLWLTSFDILGDGNEMAIGGR
                     TLRAELVPVYVQRLSREQVMKGRQFAFLQISQPQAEGKKPASLPYLEFSLQSSEQSRP
                     AGSVKPANLTTLPLSARGQELK"
BASE COUNT         1598 a         2321 c         2260 g         1769 t
ORIGIN      
        1 ccgaaaccgt gcacatcgtc gatcagcagc caggtatcga aacgctcgca caaggcaagc
       61 aaatcaggca gcggcgcgat gtcgccatcc atgctgaaca cggcatcggt cattaccagc
      121 ttgcgtttgg tgccggatgc agtcagccgt tttcccagcg cccgcatgtc gagatgcgga
      181 tagcggtgaa atttcgctct cgaaagaagc gcggcatcat tcaacgacgc atggttaagc
      241 ttgtcggcaa acaaagcatc atcgcgcccg agcagtgccg tcaccacgcc gaggttcgcc
      301 atgtagccgg tggaaaacag cagcgcttgc ggcaagcccg taaattgcgc gagcttctcc
      361 tcaacctgat ggtgcgcgtc aaaatgtccg ctcaccaggt gcgaagcgcc ggcacccacg
      421 ccataaagct ccgctccttc gcgtgcggct ttaatcagct ccgggtggga cgctaggcca
      481 agataatcat tgctgcaaaa tgaaaccagc tcgcggccat ccgccctgac atgtgcgccc
      541 tgctggcttt gcacgatgcg ccggtggcgc agcagcccct tggtttccaa gcgctgcagt
      601 tgaccgcgaa gttgggctaa agacatcgga caaaccgatc cggagccggt cgaaggaggc
      661 gtgccggaag attacatcgg atgcatgccg agtttatcga gcaatgcctg gtcctgccgc
      721 atttcgggat tgccggtagt caggagtttc ccgccgtaaa aaatcgaatt cgccccggcg
      781 agaaaacaca gggcttgcat ggcttcgggc atttggctcc ggccggcgga aaggcgaacc
      841 atcgctttgg gcatggtgat gcgcgcgcaa gcgatggtac gcacgaattc cagcggatcg
      901 agtggttcgg ctccgtaaag cggcgtgccc tcgacctgca ccagattgtt gatcggcacg
      961 ctttccggat aaggatcgag actggcgagc tgcgcgatca gcacggcgcg gctgcgacgc
     1021 gtctcgccca tgccgacgat gccgccgcag cagacatgga tgcccgctcc gcgcactcgc
     1081 gaaagcgtat ccagacggtc ccgatactcg cgtgtcgtaa taattttccc gtaaaaatcg
     1141 ggcgcggtgt cgaggttatg gttgtagtaa tcgagccccg cttcgcgcaa ttgctccgcc
     1201 tggccttcct tcaaaagccc gagcgttgca caggtttcca aacccagggc gcgcacggct
     1261 ttaatcattt cgattacctt ggctagatct ttttgcttgg ggccgcgcca ggcggcgccc
     1321 atgcaaaaac gcgtcgctcc attgtctttg gctgcgcgcg ccgccgcgat cacgccggca
     1381 acgggcaaaa tatcctcatc ggcgacgccg gtgtgatagc gcgccgcctg cggacaataa
     1441 gcgcaatctt ccgggcagcc gccggtcttg atggaaagca gagtggaaag ctgcaccgcg
     1501 ttggcatcga agtactggcg atgtatttgc tgggcgcgat agactaggtc gttgaacggc
     1561 aactcgaaca aagcttcaac ttcgtcaacg cgccatactt gacgtatttc tgctatttgt
     1621 gtggcgggct tcattacaat gtccggttcg cggatttgca gcgcctcggc taaacaataa
     1681 aattcgtcaa atcatcaagg atttccgttc aattgtcaat ttctattggt aaatttttaa
     1741 acaactgcct aaatattccg caaagactgt tgcaggcaga gtgtctgctt tgcggtgcgg
     1801 ccgttgccaa tcaggatttg tgccaagctt gccttgaaca tttgccgtat ctcgcccctg
     1861 cttgctgcaa ggtgtgcgcg gtgccgctat cgcaaggcga aatctgcggt gcgtgcttga
     1921 gcaagccgcc agcttttgac cgcacttacg ctgcattcag ctacagtttt ccggtagacg
     1981 ccttaattca ggcactcaag tatcggggaa acttcgcggt aacgccgttg ctcgcgagcg
     2041 cgctggagcc cattgcccga agcgcatctc cagtcgattg tctgattccg gtgccgttgt
     2101 ctgcggcgag actgcgcaaa cgcggcttca accaggcgct ggaaatcgcc aggccggtgg
     2161 cgcgcgcaat cggagttgcg ctcttggcga ccggctgttc ccgggtgctc gattgcgcgc
     2221 cgcaagcggg attaccgtgg aaagagcgcg tcaaaaatat ccgtggcgcg tttgcctgcg
     2281 aagccgattt atccggaaaa aaagtggcgc tgatcgatga cgtgatgacc accggcgcaa
     2341 ccctcgacga gcttgccaag gtggtgcgca ggaaaggtgc ggttgaaatc agcgtttggg
     2401 tggcggcgcg aacgctgccg ccttaaatgc agcaatcagc cagtcagcta tcagtaaaac
     2461 caatcccagc caaaaccgaa tgctgatagc ttatttaact tgcctcaagc gctgctcgac
     2521 atacgcctgc aactccggac tgagcgcgcc tgaaatcatg gcctgctgaa aagcgtcccg
     2581 cgcctcgggc aggcgctgca aagcctgcag ggaaatcccc agccccatat accacacgcc
     2641 tgcgggtttc agttgcaacg cggcttgata ttgctccact gcttggctat gccgcgactc
     2701 acgctgcagc agggcggcaa ggaaagcgcg gtaatccgcg ttctgggcgg catgaggcag
     2761 tgttttctgc agggtttcca gcgcggcctg cgtttcgcct ttctccacct gaatgcgcgc
     2821 aagtatcatc gcaaatcccg cttgtccgga ctcctgatcg agtccttcac gcagcagatt
     2881 ttccgcatca tttgcgcgct tctcctgcag caacaagccc gccagcgcca gccgcgcggc
     2941 ggcgtagttg gagttgaggc gcaaggcttg ccggaagctt tcttccgctt cttgaatttt
     3001 gccctgctgc aagtgtgccg ccgctttgcg gtattcattc tcggcctgct gctgtggcgt
     3061 ttcctgccgc atctgtttgt tgatcgccgc tacgctcgac ggtgtgggtt tcgacttggc
     3121 tttgacggga ggttcattgg ccggtttggc cgccggtttg tccggcacca gctgttcagg
     3181 ctcaggagga gggttgttga gttccaagct caggcgcgaa gcgagtttaa acagaggttc
     3241 agcgaccacc agatcttccg ccaccgtttt ctccccggtt gcaagcttgg ctggaggcgc
     3301 agcgggtaca gggacagaag cgttggcaat ggctggcgtg ggctcttcct cgcggagcaa
     3361 ctgccaggcg aaaaccccaa ccactaaaat caacaagacg agtaacaccg cccagcgcgt
     3421 agacatcgag cttcgcgtcg ggggcaaggc gcgggcttgg ctcagcacac catcgagacg
     3481 cggcacatgc cgcttctcca ggtccagcag catctgattg atgatgctca ttgcaggaac
     3541 gtccaaccga tgccgcttaa tgacaccaga agcattgcaa aagccagcca ccaccagggt
     3601 tcgatgccgt ttttcgccgc cggcgtgtcc gtggcggcga tgcgcacatg acgcggcacg
     3661 acttcatgac cgccttcacc ataggtaagc agcagcgatt tgtgcgccag aatattgacc
     3721 aaccgcggga tgccgccgct caacctgtgc atcaatctga ccgcacgccg ggtgaaaatg
     3781 cggctgccgt gatagccggc gacgctcagg cggtgcacaa gataatgatc gagctcgctc
     3841 ctgtcgagtc cgtcgaggcg gcagtggaaa gtgatgcgct ggcgcaactg gcgcaccgag
     3901 gggtgcgcca gcttttcatc aagctcaggc tggccaaaca ataccacctg caccagcttt
     3961 cttttttccg tttccagatt ggtgagcaaa cgcaacgctt caaggctctc ctgcggcatg
     4021 gcttgtgctt cgtcaagaca aatcaccacc tgtttcccct gccgcgcaaa tctcagcaag
     4081 gccagcgtca gcgatttaag aagctggtgc atgtcggcat ctttttgcag tggcgttctg
     4141 agttcgtctg caagcgcgag caacagcgag cgcggatcga ggtaagggtt gagaatatag
     4201 gcggaaacaa aggattcacc taggctcgcc aggaaacggc ggcaaagcag ggttttgccg
     4261 gcgcccacct cgcccgtaat tttcatgaag ccttcgccgc ttttcgcggc gaccaagagc
     4321 gtattcagcg cctgctgatg cgcggtgcag gcgaaaacga agctggtgtc gggagtaagg
     4381 ccgaaagggg gctcgcgcaa accaaaatgg gcacggtaca ttttctcagt cctgtattcg
     4441 agctttaagc ctcatagctc acctcaaaat cccggggcca aggccgttat ttccctgcgc
     4501 tgtcatccgt tttactggag gaagagtcgc gcctcaggct ttgcacccgt tcgcgcgcct
     4561 gcaggatgtc ttcctgccac tccgtgtcat tgtgaatcac ggttggtttg agcaaaatca
     4621 ccagttctcg tttttgcgag accttgttat tctcgcggaa caagtttccg gcaaccggga
     4681 gatctcccaa cccgggaatt tgagagcggt ccgttgtttg ggattgcgta atcagtccgc
     4741 ctatcgcgac aatctggccg tcccgcgcgc gaatcacact gtcggtttcc tgaatggcgc
     4801 ttctcggcac cggaatgctt attgaaccta aagtccctcc cagtgtaatg ttcctttgta
     4861 cttgccgcac atcgctgact gacgggtgga tatgcaaaat gatattgttg tcttcgtcaa
     4921 tccgtggcgt aacatccagc gcgatgccgg agaaaaacga ctgaagggta acggtcggag
     4981 tggtggtggt ggcggtgccc gtggtagtgg ttgtgcttac attggttaca aacaactcgt
     5041 cctgtccgac tttcagcacc gctttctggt tgttcaaagt ggcaatacgc ggactggaca
     5101 acacgtgcac attgccctgg gtttccagaa agctgatcag ggcggcaaaa ttactggtct
     5161 ggaacgccag tccaaatagc gcgccggctg cccctgcggc gcttgaaata tcggttcccg
     5221 gggctgagat gatgggtgtg ccggctatcg tgacattgcc aagcgttcca gcgctcaatc
     5281 caccggttgg ggtggcggtc gttccacctg tcaaggtgcc ggtcgtgctt aatgtcgtcc
     5341 ccggagtaag ttgtccgacc gatccccggc tattgttggt attcctgaat gcagcccaat
     5401 tgatgcccga ctggaaccca tcgttaagct ggacttcaag aattttagct tcgagtatga
     5461 cctgcctttc tacgcttacc tgcgccgctt taagataggc ggcaacattg cgcaattccg
     5521 caggcattgc gcgcaccacc accacaccgg actgcgggct gaccaccacg tggcggcctt
     5581 ccgctttacc tatgatggca gtgagcgctt tggtcagctc ctcccagaag tcggtagtgg
     5641 acgtcgtggt gattttactg ctttccaccc cgacaccccc tccgccggcg ccagtcgtgg
     5701 tcacgatggg agtgactgca actccacccg cacccgtagt aatcgcgcct gaggaggtca
     5761 cgcgaatatc ggaactgccc ttgcgcttgc cttcaaggta attcacctga aacacgcgcg
     5821 tctgcagggt caaggactgg ataaatatcc gcgtgccgtc cactttgtac tcatagccgt
     5881 agagctcgcg tatggcgtcc agcgcgtcga atacggtaac gtctttgaga ttgagcgaaa
     5941 tgggctctct gacgccggga tgcaccagca tgctgtaagg ggtgccggaa accaccgcga
     6001 ggaacacttg attggcgggc gcgttgttca ccaccagatc aaaccgcgtc tcggcggcct
     6061 tgccttcggg tttcggcagc tccaccgtga gcggaggaag taaagccctg ctcaccgcct
     6121 caggctgcgc gggttcgggc cttttttcga ctgctttgtc gagttcggca ttgatctgat
     6181 cgagggtcgt atcgtaacgc gccatttggg cagcacaccc tgtcagactt accgtcaata
     6241 agatcaggaa cgttttcctc aaatcattcc tctccgtggc ttacgccacc gcggcgggtt
     6301 ttcttttccg ttactgcgcc ggagtagccg gtcttcttct cgacatccgg aaaaagtcgc
     6361 agcgtctgca gattgccgcc agtattcagc accacctcgc tttcgcttat cttgaccacc
     6421 cgggccttgc cgtactgacc acccagcgtg acaatttcgc cactgataat ggccaccttg
     6481 tacaccggcg aaatcagcac ggactgcagc accgggccgg tttgcgcgcc gtttgggctg
     6541 accacggacg cttccaactc ggaaggcggg cgtgtggggt ccggcaaatt ctcgccgtag
     6601 cagttgatgg ccggtaccag gaataaaagc gcaaccaata aaagggcgat ggaatttgtc
     6661 agctgcgcgt tccaagtctc gagtttcgag ttgagcggaa aatgcggtgc actgggtttc
     6721 tgaacctgga acctggaacc cggaatccga aaccgtttta gactctcatc catatccggt
     6781 ccagactcag cgtgtagagc gtcaaactca ggcgcgagga aggataggtc tcgacgctca
     6841 agctgacctt ggcccagaac atctgtgacg gcagcatttc cagttccgtc acatattcga
     6901 gcaggtcgag atagccgccg cgcacggtga tttcgacccc gtgcttgaac atttcctttt
     6961 cgtcccggcc ttcctttccg ctcaaggtac tcgcctgcaa agtctgcaag gataccagtt
     7021 gcaggcggcg gttgcggttg agaatttctt ccaagagcgc cgcaattttg tccggcggaa
     7081 tcaaatgttg ctgcttgccc tgcagatacg cctcggattg cgccagctgc tgtttcagcg
     7141 cttcctgttt ggcgcggttg gcggcgtcgg gatcgcttcc gggctcgaca atagcctgga
     7201 tttgcgcctg gatgctcttg atctggtttt gcttctgcac gatctgctgg gaaagaatct
     7261 tttgcctggc gaacagcgga tccaaaaaca ccgtgaaaac cagggccacc agaatcaacg
     7321 ccagcatgcc gaaaataagc gcgcgctcgc gcaagttcat gtcgtcgatt ttaccggcca
     7381 gtgttttcca atcgtatttc atttcagttc ctgtccccga gcggatagcg ggagcgtcgt
     7441 aagattggcc ggtttcaccg aacccgccgg tcgcgattgc tcggaagatt gcaggctgaa
     7501 ttcaagatag ggcagactcg ccggcttttt cccctcggcc tggggctggc tgatttgaag
     7561 aaaggcgaac tggcggcctt tcatcacctg ctcgcggctc aggcgttgca cgtacaccgg
     7621 caccagttct gcacgcaagg tccggccgcc gatggccatc tcgttgccgt cgcccaggat
     7681 atcgaacgag gtcaaccaca agccgctcac cgactgccgt gcgaaagccc gcatatagcg
     7741 cgagtagcca ttggtgctgc cgagcgctcc gcttttaagg gtttccacga tctcctggcg
     7801 cgctttcacc tgcgcttcca gcctgccgat ttcctgctcc agaaacgtgc tttttttcag
     7861 gggtgcgtat tgagcggcaa acttctcgag acgcgcctgc tcagccgcca attttttctc
     7921 ggtttcctgc gcctgcacgc tcaaggcg
//
LOCUS       DATIOW010000107         3725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_966135, whole genome shotgun sequence.
ACCESSION   DATIOW010000107 DATIOW010000000
VERSION     DATIOW010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966135"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /gene="nuoE"
                     /locus_tag="VLL03_03490"
     CDS             complement(<1..347)
                     /gene="nuoE"
                     /locus_tag="VLL03_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004550708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HSS46461.1"
                     /translation="MLSPEALAKIDKAIVKYPPNQKQSAVMAALTIAQDEKGWLSTET
                     MDFIAQYLGMPPIAVYEVATFYTMYDLQPMGKYKISLCTNLPCALSGANAAADYLKKK
                     LAIAFNETTADGQ"
     gene            complement(496..1749)
                     /locus_tag="VLL03_03495"
     CDS             complement(496..1749)
                     /locus_tag="VLL03_03495"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HSS46462.1"
                     /translation="MAEIRNYTMNFGPQHPAAHGVLRLVLELDGEVIERADPHIGLLH
                     RATEKLAEHKTYLQSVPYMDRLDYVSMMCNEHAYVMVIEKLLELEVPVRAQYIRVMFD
                     EITRILNHLLWLGAHALDVGAMTVFLYAFREREDLMDCYEAVSGARMHAAYYRPGGVY
                     RDLPDAMPQYRASKIHSAAQVAKLNENRQGSLLDFIEDFTRRFPAYIDDYETLLTDNR
                     IWKQRTVGIGVVSAERAMALGFTGPMLRGSGVEWDLRKKQPYEIYDKMEFDIPVGVKG
                     DCYDRYLVRIEEMRQSNGIIKQCIEWLRKNPGPVITDNHKIAPPSRVEMKQNMEELIH
                     HFKLFTEGFHVPEGEAYAAVEAPKGEFGIYLVSDGANKPYRLKIRAPSFPHLAALDEM
                     SRGHMIADVVAIIGTQDIVFGEIDR"
     gene            complement(1832..2440)
                     /locus_tag="VLL03_03500"
     CDS             complement(1832..2440)
                     /locus_tag="VLL03_03500"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HSS46463.1"
                     /translation="MTAKLENLRACLNTILGERIIKLSEAAGEITVTVRSSDWLSAAQ
                     ALRDDPNLRFEQLIDLCGVDYQDYGEGAWSGPRFAVVCHLLSLTHNWRLRFKAFAESD
                     DFLAFDSLLPLWPSANWFEREAFDLFGIVFIGHPDLRRILTDYGFVGHPFRKDFPLSG
                     NVEMRYDPDQQRVIYQPVSIEPRDEVPRIVREENYANVEPKS"
     gene            complement(2735..3211)
                     /locus_tag="VLL03_03505"
     CDS             complement(2735..3211)
                     /locus_tag="VLL03_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HSS46464.1"
                     /translation="MGIEGVLEKGFVTTTADKLINWTRTGSLWPMTFGLACCAIEMMH
                     AGASRYDLDRFGIVFRPSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGIIQLQNKIKRTNTIAR"
     gene            complement(3202..3558)
                     /locus_tag="VLL03_03510"
     CDS             complement(3202..3558)
                     /locus_tag="VLL03_03510"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HSS46465.1"
                     /translation="MLENYFPILLFIIVGLAVGVGPVVLGWLIAPNRPDSEKLSPYEC
                     GFEAFEDARMKFDVRYYLIAILFILFDLEIAFLFPWAVALKEIGTFGFVAMLVFLAVL
                     VVGFIYEWVKGALEWE"
BASE COUNT          827 a         1047 c         1058 g          793 t
ORIGIN      
        1 aactgcccgt ccgccgtggt ttcgttgaaa gcgatagcga gctttttttt cagataatcc
       61 gccgcggcat tggccccgga cagcgcgcaa ggcaggttgg tgcaaagaga aatcttgtac
      121 ttgcccatcg gctgcaggtc atacatggtg taaaacgtcg ccacttcgta caccgcaatc
      181 ggcggcatgc ccaggtattg cgcgataaaa tccatggtct cggtggaaag ccagcctttc
      241 tcgtcctggg caatcgtcag cgccgccatt accgcagatt gtttctggtt gggcggatat
      301 ttgacgattg ctttatcaat tttcgccagc gcttcgggtg acaatatcat cggtgcgttc
      361 ataaccgggc tcctgaaaag ctccaaagcg caggcggaac gcgacgcgag cgggcgagaa
      421 cagcggagtt tacaaagtgg taaatgagca tgttcaagac cgtgagcaac aaagttatgc
      481 caagcgacga gcttttcatc tgtcgatttc cccgaacacg atatcctgtg tgcctataat
      541 cgccaccacg tcggctatca tgtggccgcg ggacatttca tccagcgcgg cgagatgcgg
      601 gaaactgggc gcgcggattt tcaggcggta aggtttgttt gcgccgtcgg aaacgagata
      661 aatgccaaac tcgcccttgg gcgcctcaac tgcagcatag gcttcgcctt cgggcacgtg
      721 aaaaccttcg gtgaagagct tgaaatggtg aatcagctct tccatgttct gtttcatttc
      781 cacgcgcgag ggcggcgcga tcttgtgatt gtcggtgatt accggaccgg gattctttct
      841 caaccattca atgcactgtt tgatgatgcc attggattgg cgcatttcct cgatgcgcac
      901 cagataacgg tcgtagcaat cgcccttgac tcccaccgga atgtcgaatt ccatcttgtc
      961 gtagatctcg tacggctgct tcttgcgcag atcccattcc acgcctgagc cgcgcagcat
     1021 cgggccggtg aaacccaacg ccatggcgcg ctcagcggag accacgccga tgcctacagt
     1081 acgctgcttc cagatgcgat tatcagtgag cagcgtttcg tagtcgtcta tgtacgcggg
     1141 aaagcgcctg gtgaagtcct cgatgaaatc gagcagcgag ccctggcggt tttcgttgag
     1201 cttggcaacc tgtgcggcgc tgtggatttt cgacgcccgg tattgcggca tggcgtcggg
     1261 aaggtcacgg tagactccgc ccggacggta gtaggcggcg tgcatgcgtg cgccggaaac
     1321 cgcttcgtag cagtccatca ggtcttcgcg ctcgcggaag gcgtacagga acaccgtcat
     1381 cgcgcccaca tccagcgcgt gcgcaccgag ccacaacaga tgattgagaa tgcgcgtaat
     1441 ctcgtcgaac atgacgcgaa tgtattgcgc gcgaaccggc acttccagct cgagcagctt
     1501 ttcgatcacc atcacgtagg cgtgctcgtt gcacatcatc gagacgtaat cgaggcgatc
     1561 catatacggt accgactgca gataggtttt gtgctcggcg agtttttcgg tggcgcggtg
     1621 caaaaggccg atatgcgggt cggcgcgctc aatcacttcg ccgtcaagct ccagtaccag
     1681 ccgcaacacc ccgtgcgccg ccgggtgctg cggcccgaag ttcatcgtgt aattccggat
     1741 ctctgccatt ggcattcacc gcggaggcgc ggaggcgcgg agaaaacttg cccgcttgag
     1801 attcattctc ggcgtctctg cgtctctgcg gttaactctt tggttccaca tttgcatagt
     1861 tttcctcgcg cacgatgcgc ggcacttcgt cgcgcggctc gatggacacc ggctggtaaa
     1921 tcacgcgctg ctggtccggg tcgtagcgca tttccacatt gccggaaagt ggaaaatcct
     1981 tgcggaatgg gtggccgaca aagccgtaat cggtgaggat gcgccgcaag tcgggatgcc
     2041 cgatgaacac gatgccgaac aaatcgaacg cttcgcgctc gaaccagttg gcggaaggcc
     2101 aaagcggaag cagcgaatcg aacgccagga aatcatcgct ttcggcaaaa gccttgaaac
     2161 gcaaccgcca gttgtgggtg agcgatagca gatgacagac caccgcgaag cgcggtccgc
     2221 tccacgcgcc ttcaccgtaa tcctggtaat ccacgccgca caggtcaatc aactgctcaa
     2281 atcgcaagtt cgggtcgtca cgcagtgcct gtgctgccga caaccagtct gaagatctca
     2341 ccgtgactgt gatttcaccc gccgcttcgg aaagtttgat gatgcgctcg cccaaaatag
     2401 tgttaaggca tgcgcgaaga ttttctagtt tggcggtcat tttaatgagc cctaaattag
     2461 gttatttgag acctctgcaa aacaagtatt tggtttcgag acgaggcgcg agctttgatc
     2521 gaagggcgcc gcgacacggt tgcgaagccg agtgcgggag cggtgacaag caccctgggt
     2581 aagcactgcc atttgcagcg cccagctacc cggtctccgc cccgagcttc gccgccgtga
     2641 gccagggtgc agtgcatatg agaaatacat gagcattcga gcaaagtgag caacgaagga
     2701 tcggggccaa agacgccgtt ttgcagaggt ctcatcagcg ggcgatagtg ttggtgcgtt
     2761 ttatcttgtt ttgcaattgg ataatgccgt aaagcagggc ttctgcagtg ggcgggcagc
     2821 ccggcacata aatatccacc ggcacgatgc ggtcgcagcc gcgcaccacg gaataggaat
     2881 aatggtaata gccgccgccg ttggcgcacg agcccatgga tattacccag cgcggctcgg
     2941 ccatctggtc atacactttg cgcagcgccg gtgccatctt gttgcacaac gtgccggcga
     3001 cgatcatcac gtccgactgg cgcggactgg gacggaacac gatgccgaag cggtcgagat
     3061 catagcgcga agcgccggcg tgcatcattt ctatcgcaca acaggcgagt ccgaaggtca
     3121 tcggccacaa tgagccggtg cgcgtccagt tgatcagctt gtccgcggtg gtggtgacaa
     3181 aacctttttc cagtacgcct tctattccca ttcgagagcc cccttcaccc actcatagat
     3241 gaatcccacc accaggactg ccaggaacac cagcattgct acaaaaccaa acgtgccgat
     3301 ttccttgaga gccactgccc agggaaagag aaaagcgatt tccagatcga acagaataaa
     3361 cagaatcgcg ataaggtaat agcgcacgtc gaatttcatg cgcgcgtcct caaatgcttc
     3421 gaatccgcat tcataagggg agagtttttc gctgtccgga cggtttggcg ctataagcca
     3481 gcccagaacc acggggccga caccgacagc aaggccgacg atgataaaca acaagatcgg
     3541 aaagtaattt tccagcatat acggtccggg gttgtacggg cgccaacgct ggcgaacaac
     3601 ctccttatgg tttagatccg gtgtcgctca cgagttcagc gcgctgccgg aattctcaat
     3661 tcgtggtgcc gatgatgaga ctcgaactca tacggctctc gccaccgccc cctcaagacg
     3721 gcgtg
//
LOCUS       DATIOW010000108         4824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_967390, whole genome shotgun sequence.
ACCESSION   DATIOW010000108 DATIOW010000000
VERSION     DATIOW010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967390"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /locus_tag="VLL03_03515"
     CDS             <1..1389
                     /locus_tag="VLL03_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HSS46466.1"
                     /translation="GLQRILVLNGVDAADRNDARLAAVEDWLPEAGAQEMETMGKTDE
                     LATIVYTSGTTGRPKGVMLSHYNILSNVFSTLQSIPARTDDLFLSFLPLSHTFERTVG
                     YYLTVMAGATVVYARSIPQLAEDLACIRPTLLISVPRIYERVYAGIKAKLDEGSAVKR
                     SLFNLTVKVGWSRFEHAQKRGPWRVSHLLWPLLNRLVASKIMARLGGRLRGTLSGGAA
                     LPPDISKIFIGLGLPLVQGYGLTETSPVACCNRIDNNVPASIGLPIPGVEVKIGEKNA
                     LLIKGPNVMLGYWNNPEATKAMIAPDGWLNTGDTARIDEQGRVYITGRLKEIIVMSNG
                     EKIPPVDIETAIARDPLFEQILVLGEGKPYLAVLAVLNAEQWKKTAQEAGLDPQSATA
                     LHDKAAEKLVLERIARQIRDFPGYAQIRRAALLLEPWTVENGLLTPTLKLRRPRVMEQ
                     YGDKIAKLYEGH"
     gene            1390..1824
                     /locus_tag="VLL03_03520"
     CDS             1390..1824
                     /locus_tag="VLL03_03520"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HSS46467.1"
                     /translation="MTKQRLEKTVLPGKQPALRVVPMPSDANYAGDIFGGWIMSQVDI
                     AGSIPAMRRARGRVATVAVNSFVFKQPVFVGDLVSLYADIVRIGRTSITVDVGVYAQR
                     NPLKEECVKVTEATLTYVAVDETRKPRVVPEVEQPLNPQEVK"
     gene            1890..3197
                     /locus_tag="VLL03_03525"
     CDS             1890..3197
                     /locus_tag="VLL03_03525"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HSS46468.1"
                     /translation="MRKDLLFKPAVAVAVAAMFGAAAHGALAAGFALNEQSVSGLGVA
                     FAGGAASGEDASTVFYNPAAMTRISGTQAVVGVHGILPDATFTNQGSVTVGGPLTGGN
                     GGDLAKSVGVPNFYLVTDINPNIKYGFGVSVPFGLTTAYDGNWVGRYQALKSEIKTVN
                     LNPSLAYKVNERISVGAGFNAQYINAELSNAIDFGLLLGAPQSRDGAVNVSGNDWSFG
                     YNLGVLFQLPTDTRVGLAYRSKIHHTVVGNASFTNVPPVPAIANNPRFQSGGINAAVT
                     LPDSMSLSVVQGVGERWELLSDVTFTRWSYFNRLVINFNNAPSTPSITQENWRNTFRA
                     SIGATYRYSDVLKLRGGVGFDQSPVRGAFRTARIPDNHRKILAAGANYKVSKAGSVDF
                     AYQRLFANDAAINNNQLATGGGRLIGNYSLDVNILSLQYNHNF"
     gene            3245..>4824
                     /locus_tag="VLL03_03530"
     CDS             3245..>4824
                     /locus_tag="VLL03_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HSS46469.1"
                     /translation="MSQDSPFIVRKAAVLGAGVMGAQIAAHLVNANVDAILFELAAKE
                     DGPNANVRKAVEGLKKLEPSPLATPGRAVYIHQANYDQHLDLLKECDIVIEAISERMD
                     WKADLYKKVAPYLSEHAIFASNTSGLSINRLAQAFPEAIRHRFCGVHFFNPPRYMHLV
                     ELIPCAGSDPQLLDRLETFLVTALGKGVIRAKDTPNFIANRVGSFGMLATIHYTRQFG
                     LGFDVVDALMGPLIGRPKSATYRTADVVGLDTFAHVINTMRDRLPDDPWHPYYQVPAW
                     LKMLIDKGALGQKTKAGIYRKIGKDIQVFDLDKGDYRLSDGTADEGVKEILKNKNPGE
                     KFAQLRANSHPQAQFLWAIFREAFHYCAAHLESIADNARDVDLAMRWGFGWDRGPFET
                     WQAAGWREVANWINEDIAAGKSMTKASLPAWVLERNGAHEPRGSYAPREKALKARSAL
                     SVYRRQLYPDALLGEERKYGETVFETDAVRMWHGGDDIAIVSFKSKMHSIGDDVLDGV
                     MEAIDRAEKNFAGLVIWQT"
BASE COUNT         1038 a         1322 c         1495 g          969 t
ORIGIN      
        1 ggcttgcagc gcattttggt gctgaatggc gtcgatgccg ctgacagaaa tgatgcgcgc
       61 cttgcagcgg tagaagactg gcttcccgaa gcaggcgcgc aggaaatgga aacgatgggg
      121 aaaaccgacg agcttgccac catcgtctat acttccggca ctaccggccg gcccaagggc
      181 gtgatgctct cgcactacaa catcctttcc aatgtcttca gcacgctcca gagtattccg
      241 gcgcgcaccg atgatttatt cctgtcgttt ttgccgctct cgcacacctt cgagcgcacc
      301 gtgggttatt acctgacggt gatggcgggc gctacggtgg tctacgcgcg ctcgataccc
      361 cagcttgcgg aagatttagc ctgcatacgg ccgacgctct tgatttcggt gccgcgaatt
      421 tatgagcggg tatatgccgg catcaaggcc aagctggatg aagggtcggc ggtcaaacgc
      481 tccctgttca atttgaccgt aaaggtgggc tggagccgct tcgagcatgc gcagaaacgc
      541 ggaccctggc gcgtatcgca tctgctgtgg ccgctgctca accggttggt ggcctctaag
      601 attatggcca gactcggcgg cagacttcgc ggtacgctat ccggcggggc ggcgctgccc
      661 cccgatattt ccaaaatttt cattggtctg ggtctgccgc tggtgcaggg ttatggcttg
      721 accgagacca gtcccgtagc gtgctgcaac cgcatcgata acaacgtacc cgcgagcatc
      781 gggctgccga taccgggcgt ggaagtaaaa atcggagaga aaaacgcact gctgatcaaa
      841 ggtccaaacg tgatgctggg ttactggaat aatccggaag cgaccaaagc gatgattgcg
      901 cccgacggct ggctcaacac cggggacacg gcgcggattg atgaacaggg ccgggtttat
      961 atcaccggcc ggctcaagga aatcatcgtc atgtcgaacg gtgaaaaaat cccgccggtg
     1021 gacatcgaga ccgccatcgc gcgcgatccg ttgttcgagc agatcctggt gctgggcgaa
     1081 ggaaaaccct acctcgcggt gcttgcggtg ctcaacgccg aacagtggaa aaaaaccgcg
     1141 caggaagccg gcctcgatcc gcagtcggcg acggctttgc atgataaagc ggcggaaaaa
     1201 ctggtgctgg aacgcattgc gcggcagatt cgggattttc ccggctatgc gcaaatccgg
     1261 cgcgcggctc tgttgcttga gccgtggacc gtggaaaacg gactgctcac gcccacgctg
     1321 aaattgcgcc ggccgcgggt gatggagcag tacggggaca aaatcgcgaa actttacgag
     1381 gggcattgaa tgaccaagca aagactagaa aaaaccgtat tgccgggcaa gcagcccgcc
     1441 ttgcgcgtgg tgccgatgcc ttcggacgcc aactacgccg gcgatatttt cggcggctgg
     1501 atcatgtcgc aggtggacat cgccggcagc atccccgcca tgcgccgcgc gcgcgggcgc
     1561 gtcgcgacgg tggcggtgaa ttccttcgtg ttcaagcagc cggtgtttgt cggcgatctg
     1621 gtaagcttgt acgctgacat cgtgcgcatc ggccgcacct cgattaccgt ggatgtgggg
     1681 gtgtacgcgc agcgcaatcc gttgaaggaa gaatgcgtga aagtcactga agcgacactc
     1741 acctatgtcg cggtggacga gaccagaaag ccccgtgtgg tgccggaagt tgagcagccg
     1801 ctcaacccgc aagaggttaa gtgaaaagtg cgcccgactg ttgcgcgggt gttttgaatc
     1861 cagatctata cttgaacaag gaggtaaaaa tgagaaaaga tcttttattc aaaccggcgg
     1921 ttgcggttgc tgtggcagca atgtttggcg cagcggcaca tggcgcgctg gccgccggtt
     1981 tcgccctgaa tgagcagagc gtgagcgggc tgggcgtggc tttcgccggc ggcgcggcga
     2041 gcggcgaaga cgcgagcacg gttttttaca atccggcggc aatgacgcgc atttcgggga
     2101 cgcaggcggt ggtgggggtt cacggtatcc tgcccgacgc taccttcacc aatcagggtt
     2161 cagttaccgt gggcggccct ttgaccggcg gcaatggcgg cgacctggca aaatcggttg
     2221 gcgtgccgaa tttttatctg gtgactgaca tcaacccaaa catcaagtac gggtttgggg
     2281 tcagcgtgcc tttcggcctt accactgctt atgacggcaa ttgggtggga cgctaccaag
     2341 cgctcaaaag tgaaataaag acggtgaatc tcaatcccag cctggcgtat aaagtgaatg
     2401 aacggatttc agtcggcgcg gggtttaacg cgcaatatat caatgctgaa cttagcaacg
     2461 cgatagattt cggattgctt ttgggggcgc cgcaatcccg cgacggcgcg gtgaatgttt
     2521 ccgggaatga ctggagtttc ggctacaacc tcggcgtgct gttccagctt ccaaccgata
     2581 cccgcgtggg cctcgcttat cgctccaaga tacaccatac agtggtgggc aatgcgtcgt
     2641 tcacaaatgt ccccccggtg ccggctatcg caaacaatcc acgtttccag agtggtggca
     2701 ttaatgcggc agtgacgctg ccggattcca tgtcactaag cgtggtgcag ggggtcggcg
     2761 agagatggga attattgagc gatgtcacct ttacccgctg gagttacttt aaccggctag
     2821 tgatcaactt taacaacgcg ccctcaaccc caagcataac ccaggaaaac tggcgtaata
     2881 ctttccgcgc ttcaattggt gcgacctacc gctacagcga tgtattgaaa ctgcgcggcg
     2941 gggttggctt tgaccagagc ccggtcaggg gcgccttccg cacggcgcgt atacccgaca
     3001 atcaccgcaa gatcctggcg gcgggcgcaa attacaaggt gtcaaaagca ggctcggtgg
     3061 atttcgcgta ccagcgctta tttgccaatg atgccgcgat caacaacaac cagctcgcga
     3121 ccggcggcgg acggttgatt ggcaactaca gtctggatgt gaatatcctg agcttgcaat
     3181 ataaccataa cttctgacgg gcggggcttg cagtcgcccg ccggcgctaa tctcaggaac
     3241 aatcatgtca caggactcac cttttatcgt gcgcaaggcg gcggtgcttg gcgccggtgt
     3301 gatgggtgcg cagattgcgg cgcatttggt gaacgccaac gttgatgcga ttttgttcga
     3361 gctcgccgca aaagaagacg gcccgaatgc caacgtgcgg aaagcggtgg aaggcttgaa
     3421 aaaactcgag cccagcccgc tcgccacccc tgggcgcgcg gtgtacatcc atcaggccaa
     3481 ctatgaccaa cacctcgatc tgctcaagga gtgcgacatc gtgatcgagg cgatttccga
     3541 gcgcatggac tggaaagcgg atttgtacaa gaaagtcgcg ccttacttaa gcgagcacgc
     3601 gatttttgcc agcaacactt ccggcctttc catcaaccgg ctggcacagg cttttcccga
     3661 agccatccgc caccgctttt gcggcgtgca ttttttcaac ccaccgcgct acatgcacct
     3721 ggtggaactg attccgtgcg caggcagcga tccgcaattg ctggaccggc tggaaacttt
     3781 cctggtcact gcgctgggca agggcgtgat ccgggccaag gacacgccga acttcatcgc
     3841 caaccgcgtc ggctcgttcg ggatgctggc gacgattcat tacacccggc aattcggtct
     3901 gggtttcgat gtggtggatg ccttgatggg accgctcatc ggccgcccca agagcgcgac
     3961 ttaccgcacc gccgatgtgg tggggctcga taccttcgcg catgtgatta acaccatgcg
     4021 cgataggctg cccgacgatc cctggcaccc gtattaccag gtgccggcat ggttgaagat
     4081 gctgattgac aagggcgcgc tggggcagaa aaccaaggcc ggcatctacc gcaaaatcgg
     4141 caaggacatt caggtcttcg atctggacaa aggggattac cggctctcgg acggcacggc
     4201 cgatgaaggg gtgaaggaaa ttctgaaaaa caagaatccg ggggaaaagt tcgcgcagct
     4261 gcgcgccaat tcccatccgc aggcgcagtt cctgtgggcg attttccgcg aagcgttcca
     4321 ctactgcgcg gcgcatttgg aaagcattgc cgacaatgca cgcgacgtgg atctggcgat
     4381 gcgctgggga ttcggctggg atcgtggccc gtttgaaacc tggcaggcgg cgggctggcg
     4441 cgaagtggcg aactggatta acgaagacat cgccgccggc aagagcatga ccaaggcgtc
     4501 tttgcccgca tgggtgctgg agcgcaatgg cgcgcacgaa ccgcgcgggt cgtatgcgcc
     4561 gcgtgaaaaa gccttgaaag cgcgctcggc tctgtccgtg taccggcggc agctttatcc
     4621 cgatgcgctg ctgggcgagg aaagaaaata cggtgaaacg gtgtttgaaa ccgatgccgt
     4681 gcgcatgtgg catggaggcg acgacattgc catcgtcagc ttcaagagca agatgcacag
     4741 tatcggcgac gatgtgctgg atggcgtgat ggaagcgatt gaccgcgccg aaaagaattt
     4801 tgccggtttg gtgatttggc agac
//
LOCUS       DATIOW010000109         8743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1272227, whole genome shotgun sequence.
ACCESSION   DATIOW010000109 DATIOW010000000
VERSION     DATIOW010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8743
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272227"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /gene="plsY"
                     /locus_tag="VLL03_03535"
     CDS             complement(<1..628)
                     /gene="plsY"
                     /locus_tag="VLL03_03535"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HSS46470.1"
                     /translation="MLTIVIVMGAYLLGSVSFAVLTSQLFHLPDPRSYGSKNPGATNV
                     LRTGKKAAAALTLLGDTAKGWLAVWLAKSIAPQYGLSQEVIVAAALAVFLGHLYSIFL
                     RFQGGKGVSTALGVLLALDVWLGLATLATWIMVFVISRISSLSALTAAALMPLYSLYV
                     FGLSNITLAVLSISLLLIWRHKSNIKNLIAGTESDFKKSGQRRLLQAAV"
     gene            691..1047
                     /locus_tag="VLL03_03540"
     CDS             691..1047
                     /locus_tag="VLL03_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HSS46471.1"
                     /translation="MDTIFIHEFKIETLIGIYEWELKVPQTIQLDLEIGIPQSKVYKT
                     DSINDTIDYGRVVARLQQTLSEQHFSLLERLAEHLAELIMKEFGAPWVKVSVAKLGLV
                     RGIKRLGVTIERGKKA"
     gene            complement(1044..1946)
                     /gene="xerD"
                     /locus_tag="VLL03_03545"
     CDS             complement(1044..1946)
                     /gene="xerD"
                     /locus_tag="VLL03_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HSS46472.1"
                     /translation="MSGVEESLLDEFCDALWLEDGLARNTLASYRSDLLKFVQWLSKK
                     NRIPLLDTRQQDIEDFLAHQVLAAKVRARTTSRELSSLRRFFRFALRQGRIKADPTLR
                     IDSPKLPRGLPKNLTEDDVESLLNAPRVDTPLGLRDKTMLEVLYASGLRVSELVSLKI
                     PQVSLDMGVVRVMGKGSKERLVPLGEEALTWIRRYLDEARPDLLKGKMRDALFVTERG
                     QAMTRQAFWYLLRRYCAQAAFKKPVSPHTLRHAFATHLLNHGADLRVVQLLLGHADIA
                     TTQIYTHVARERLKQLHAKHHPRG"
     gene            complement(1933..2415)
                     /locus_tag="VLL03_03550"
     CDS             complement(1933..2415)
                     /locus_tag="VLL03_03550"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HSS46473.1"
                     /translation="MHQAEIQGEYQAKLKVPFAVLGIRHQGECLTRIDFLPRQTRALS
                     PQTSFAAKVCRQLEAYLRDPDARFNLPMKLSGTPYQQKVWRSIQQIPRGRTLTYGEIA
                     RKLFSGPRAVGQACGANPIPLVVPCHRVLGSGNLGGFMNCDEGDPLVIKRWLLKHERG
                     "
     gene            complement(2549..2947)
                     /gene="rplS"
                     /locus_tag="VLL03_03555"
     CDS             complement(2549..2947)
                     /gene="rplS"
                     /locus_tag="VLL03_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HSS46474.1"
                     /translation="MDLIKQLEAEEIKRLNKSIPDFAPGDTVVVNVNVVEGDRKRVQA
                     YEGMVVAKRNRGLNSSFIVRKVSSGEGVERTFQTYSPLIASIEVKRKGDVSRAKLYYL
                     RQRSGKSARIREKLESQVMDGTLPETPGQQ"
     gene            complement(2951..3709)
                     /gene="trmD"
                     /locus_tag="VLL03_03560"
     CDS             complement(2951..3709)
                     /gene="trmD"
                     /locus_tag="VLL03_03560"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HSS46475.1"
                     /translation="MLYFDVITLFPPMFGALTEYGVTRRAREEGRYQLKVWNPRQFTT
                     NNYHTVDDRPFGGGPGMVMLAEPLEQAIEQAKQHQREAGVGQTQVIYLSPQGRVLNHA
                     RVAGLARQPGLILLAGRYEGVDERLITRQVDEEISLGDYVLSGGELAALVLMDCIIRQ
                     LPGVLGDAQSAEQDSFVNGLLDCPHYTRPEAYAEMGVPQVLLSGHHAEINRWRLKQML
                     GRTWLRRPDLLAKRGLSDEERKLLDEFKQEQGKE"
     gene            complement(3720..4232)
                     /gene="rimM"
                     /locus_tag="VLL03_03565"
     CDS             complement(3720..4232)
                     /gene="rimM"
                     /locus_tag="VLL03_03565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HSS46476.1"
                     /translation="MRKKPLEKMVAMGRVTAPYGVKGWIKIQPYTETLESLTRHTSWW
                     VGEESEWREIAVAEARPHGKTVVAKLKDVEDRAAALSLKGAHIAVKRSQLPKAKAGEY
                     YWADLLGLLVVNLDGEELGTVKNLFATGANDVLRIMGERERLIPFIPQVVCEVNLAQG
                     VVRVDWSLDY"
     gene            complement(4213..4467)
                     /gene="rpsP"
                     /locus_tag="VLL03_03570"
     CDS             complement(4213..4467)
                     /gene="rpsP"
                     /locus_tag="VLL03_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HSS46477.1"
                     /translation="MVVIRLSRGGATKRPFFNVVVADKRRSRDGKFIERIGFYDPKAP
                     EGHETLRIQMDRINHWQGRGAKLSDTVVRLIRRLGAQKAA"
     gene            4679..5536
                     /locus_tag="VLL03_03575"
     CDS             4679..5536
                     /locus_tag="VLL03_03575"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSS46478.1"
                     /translation="MPTQAIKRALYLYKDYKLKDFSKALLRKLKLPKYLGHTHFCPVC
                     NTRLKCFKPIFKSFLRKLGEYGFVYPVESFETLNVSAYSCPSCDASDRDRLYALFLEE
                     RFQTLKQGKVYKFVDFAPSLPLSRKLRTCSSLDYRSADLHRKTADDRVDITDMALYAN
                     DSIDMFLCSHILEHVPDDQKAMRELHRILKPGGFGIVMVPIITVLQDTHEDTSITSPE
                     GRWKYFGQDDHLRQYSRAGENGLVTRLQKAGFTVHLYAVEHFGAAAFDKHGIARNSVL
                     YVVEKHAAE"
     gene            complement(5673..7019)
                     /gene="ffh"
                     /locus_tag="VLL03_03580"
     CDS             complement(5673..7019)
                     /gene="ffh"
                     /locus_tag="VLL03_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HSS46479.1"
                     /translation="MLDHLAQRLSGVIKTLRGQARLTETNIQDALREVRMALLEADVA
                     LPVVKEFINRIKEKALGQEVLGSLTPGQAAIGVVHHELAQLMGSSHAGLDLATAPPAV
                     ILLAGLQGAGKTTTAGKLARILQAQKKKVLLTSCDVYRPAAIEQLRTLAKQLNVDFFP
                     VEAGSRPAMIAQSALDFARKHFHDVLLVDTAGRLAIDETMMREIGELHAALKPIETLF
                     VVDAMQGQDAVNTAKAFNDALPLTGVVLSKLDGDARGGAALSVRFVTGKPIKFSGVGE
                     KLDGLEPFHPERMASRILGMGDVLSLVEEVQRGVDQEEAKKLAQKVKQGKGFDLEDFK
                     AQIQQMRKMGGLAKLVDKLPGEMGQMAQNPQMDEKTIHRVEGIINSMTPQERAKPEIL
                     KASRKRRIAAGAGVTVQEVNRLLNQFEQVQKMMKMISKGGMGKMMRGMKGMFPGMR"
     gene            7095..7922
                     /gene="ccsA"
                     /locus_tag="VLL03_03585"
     CDS             7095..7922
                     /gene="ccsA"
                     /locus_tag="VLL03_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HSS46480.1"
                     /translation="MPAILPYLITALLYALLGFHFWRTRWQPAAPESGGIAAPMQWER
                     FAILVPFVLHSALLYHSTFTGAGVNLGVGNALSAILWLAVLLYWLVSFFYNFEGLQAL
                     VLPLAAACVLVPLLLPTTHMLSNTGLPAFHVHFLLSMLAYGLLTIAALHAVLMMLVER
                     RLHLGALPAMLNSLPPLLTMETLLFRIINVGFVLLTLVLISGILFSEELFHKPAPFTH
                     KTLFAFISWGIFAALLGGRKIYGWRGRTATRWTLAGFIALILAYIGSKFVLEVILHR"
     gene            8050..>8743
                     /locus_tag="VLL03_03590"
     CDS             8050..>8743
                     /locus_tag="VLL03_03590"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HSS46481.1"
                     /translation="MLLIISAFFSISETSMMALNRYRLRHLVQVGSSGAKLTSQLLAQ
                     TDKLLGVILLGNTLLNAAATTLSTIIAVQLFGDREIVLGLSTVCITFMLLVFSEITPK
                     IIGASQPERIALPASYLLTPLLKLSYPAVWIVNLFAKALLWLLRFKPAAEAHKLSLEE
                     LRTLVLEASHFIPQKHQSILLNLLELENITVNDVMTPRNHIEAVDIEAPQEVIRRQLA
                     TCYHTRLPVYQSQ"
BASE COUNT         1754 a         2692 c         2338 g         1959 t
ORIGIN      
        1 caaccgcggc ttgaagaagc cggcgctggc cagacttttt gaagtcactc tcggtgccgg
       61 cgatgaggtt cttgatattc gacttatggc gccagatgag cagcagactt atgcttaaaa
      121 ccgccagcgt gatgttgctc aagccgaaaa cataaagcga ataaagcggc atgagtgccg
      181 ctgcggtgag cgcggagagc gaggaaatgc gcgagatcac gaataccatg atccacgtcg
      241 ccagcgtcgc cagtcccagc caaacatcca acgcgagcaa cacacccagc gcggtggata
      301 cgcccttgcc gccttggaag cgcaggaaaa tcgaatacaa atggccgagg aaaactgcca
      361 gcgcagccgc cacgatcacc tcctggctca agccatattg cggggcgata gatttggcca
      421 gccataccgc cagccagcct ttcgccgtgt cgcccaacag cgtcaacgcc gcagcggctt
      481 tcttgccggt gcgcagcaca ttggttgcgc cgggattctt cgagccatag ctgcgcgggt
      541 caggtaggtg aaagagctga cttgtcagaa cggcgaacga taccgagccc agcaggtacg
      601 cgcccataac aattactatc gtcagcatgg caaatattct acaatccgcc tttcccgcct
      661 accaggccca aacgcacggt tgatcgccgc atggatacca tcttcataca tgaattcaag
      721 atcgagaccc tcatcggcat ttacgaatgg gagctcaaag tgccgcagac catacagctt
      781 gacctggaaa tcggcatccc gcaaagcaag gtgtacaaaa ccgattcgat caacgacacc
      841 attgattacg gcagggtagt ggcgcgcttg cagcaaactc tgtccgagca gcacttctcg
      901 ctgcttgagc gcctcgccga acatctcgcc gagctgatta tgaaggaatt cggcgcccca
      961 tgggtgaaag tcagcgtcgc caagctcgga ctcgtgcgcg gcatcaagcg tctcggtgtg
     1021 accattgaaa gaggcaaaaa agcctagccc cgtggatgat gcttggcgtg cagctgcttc
     1081 agtcgttccc gcgccacgtg agtgtaaatc tgcgtggtgg caatatcggc gtggcccaag
     1141 agcaactgca ccacgcgcaa atcggcgccg tgattgagaa gatgcgtagc aaaggcgtgc
     1201 cgcaaagtat gcggtgaaac cggtttcttg aacgcggcct gcgcacaata gcggcgcagc
     1261 aaataccaga aagcctggcg cgtcatcgcc tgcccgcgct ccgtgacaaa cagcgcatcc
     1321 ctcatttttc ctttgagcag atccggccgc gcttcgtcca gatagcgcct gatccatgtc
     1381 agcgcttcct cgcccagcgg caccagccgc tccttcgatc ccttgcccat tacccgcacc
     1441 acgcccatgt cgaggctgac ctgcggaatt ttcaggctca ccagttccga cacgcgcaag
     1501 ccgctggcgt agagcacttc cagcatggtt ttgtcgcgca agccgagcgg cgtgtccacc
     1561 cgcggcgcat tgagcaagga ctcgacatcg tcttccgtca ggtttttggg caagccgcgt
     1621 ggcagcttag gggaatcgat gcgcagagtg ggatcagcct tgatcctgcc ctggcgcagg
     1681 gcaaagcgga aaaaacgcct gaggctggaa agctcgcggc tggtggtgcg tgcccgcact
     1741 ttggcggcca gcacttgatg cgcgaggaaa tcctcgatgt cctgttgccg ggtatcgagc
     1801 agcggtatgc ggttcttttt gctcaaccac tgcacgaatt tcagcaaatc actgcggtaa
     1861 ctcgcaaggg tattgcgcgc aagcccgtcc tccaaccaca gggcgtcgca gaattcatcg
     1921 agcaggcttt cctcaacccc gctcatgctt gagcagccag cgcttgatca ccagcgggtc
     1981 accctcgtcg cagttcatga aaccgcccag gttgccgctc cccagcacgc ggtggcaggg
     2041 cacaacaagg ggaatcggat tggcgccgca tgcctgcccc accgcacgcg gccccgaaaa
     2101 cagcttgcgc gcaatctcgc cataggtcag cgtgcgcccg cgtggaatct gctggatgga
     2161 ccgccatact ttttgctgat aaggcgtgcc gctcagcttc atcggcagat tgaaacgtgc
     2221 atccgggtcg cgcaggtagg cttcaagctg acgacacact tttgcggcaa aggaagtttg
     2281 cggagaaagc gctcgggtct ggcgcggcag gaaatcaatc cgcgtgaggc attcgccttg
     2341 gtggcgtata ccgagcaccg caaaggggac tttaagcttc gcctggtatt cgccttggat
     2401 ttccgcttga tgcatacgca aaagatagcg cagttgggcg caaactacaa tcgcttgagt
     2461 gcctgcccga ccgcttccag ttgcgcgggc ccgaaaaaca aaaacccagc gggatcaccg
     2521 ccgcgttttt agtcgagctc ttgcgagatt attgctggcc gggagtttcc ggcagagtcc
     2581 cgtccatgac ctggctttcc agtttttcgc gaatgcgtgc cgacttgccg gagcgctggc
     2641 gcaggtaata gagcttggcg cgggatacat cgcctttgcg cttgacctcg atgctggcaa
     2701 tcagcggtga ataggtctgg aaagtccgct ccacgccttc gccggacgac actttgcgca
     2761 cgatgaagga ggagttgagc ccgcggttgc gcttcgccac caccatgcct tcataagcct
     2821 ggacgcgctt gcggtcgcct tccaccacgt tgacgttcac caccacggta tcgccggggg
     2881 caaaatccgg gatgctcttg ttaagccgct tgatttcctc ggcttcgagc tgtttgatca
     2941 ggtccatcgt ttactccttg ccctgttcct gtttgaattc gtcgaggagt ttgcgctcct
     3001 cgtcacttaa cccacgcttg gcgagcaaat ccgggcgccg cagccaagta cgccccagca
     3061 tttgcttgag ccgccagcgg ttgatttccg catgatggcc ggagagcagg acctgcggta
     3121 cgcccatctc cgcatacgct tccgggcgcg tatagtgcgg gcagtcaagt aggccgttca
     3181 cgaacgaatc ctgctccgcc gactgcgcat cgcccagcac gccgggcagt tggcggatga
     3241 tgcagtccat cagcaccagc gccgccagtt ccccgccgga gagcacgtag tctcccagcg
     3301 agatttcctc gtctacctgg cgggtaatca gccgttcgtc cacgccttca taacgcccgg
     3361 cgagcaatat taatcccggc tgtcttgcga gtcccgccac ccgggcatgg ttcaataccc
     3421 tgccttgcgg cgataaataa atgacttgcg tttgcccgac acccgcttcg cgctgatgct
     3481 gcttggcctg ttcaatcgcc tgctccagcg gctccgccag catcaccatt cccggcccgc
     3541 cgccaaaagg acggtcgtcc accgtgtggt aattattcgt ggtgaactgc cgcggattcc
     3601 aaacctttaa ctgatatcgt ccttcttccc gggcgcgccg tgtaaccccg tattcggtga
     3661 gcgccccgaa catcggcggg aagagcgtga tgacgtcgaa gtagagcatc ggggcgtctt
     3721 tagtaatcca gactccaatc caccctcacc acgccttgag ccagattcac ttcgcacact
     3781 acttggggaa tgaacggaat gaggcgttcg cgttcaccca ttatccgcaa cacatcgttg
     3841 gcccccgtgg caaacaggtt ttttaccgtc cccaactctt cgccgtcgag gttcaccacc
     3901 agcaggccga gcaaatcggc ccagtaatat tctccggctt tggcttttgg caattggctg
     3961 cgtttcaccg cgatatgggc gcctttgagg cttagcgccg ctgcacggtc ttccacgtct
     4021 ttcagctttg ccactactgt tttgccgtgc ggcctggcct cagccacagc gatttcacgc
     4081 cactcgcttt cttcacccac ccaccatgat gtgtgacgag tcaggctttc caacgtttcg
     4141 gtatatggtt ggatcttgat ccagcccttc acaccatacg gagcggtaac ccgtcccatc
     4201 gccaccattt tttcaagcgg ctttttgcgc accaagccgt ctgatgagcc gcaccacggt
     4261 gtcgctgagt tttgcgccgc ggccctgcca gtgattaatc cggtccattt ggatgcgtag
     4321 cgtctcatgg ccttccggcg ctttcgggtc gtaaaacccg atgcgctcga taaatttgcc
     4381 gtcgcgcgaa cgtcgcttgt ccgccaccac cacgttgaag aacggacgct ttgtggcgcc
     4441 accgcgcgat agtcgaatca cgaccatagt ccacctaccc agaaaatcca gaaaggccgt
     4501 gattttacgc tgatttacga gagcttggca aggggaaaag agcttggtgc cgcggccggt
     4561 tcagtcgcgg taatccgcat caggtctgtt tgccggctgc aaatttcaca tcggatttgc
     4621 cgcttccttt agaattccct acttgttgcc cgcgtcgcaa gcctttgaga agaaactgat
     4681 gccgacccaa gccataaaac gcgcgcttta cctgtataaa gactacaagc ttaaggattt
     4741 cagcaaagcc cttctgcgca aactgaaatt gccgaagtat ttgggccaca cccacttttg
     4801 tcccgtttgc aacacccggc tcaaatgctt caagcccatt ttcaaatcct ttttgagaaa
     4861 gcttggggaa tatgggtttg tttatcccgt cgagtcgttt gaaaccctga atgtttccgc
     4921 ctattcctgc ccctcgtgcg acgcctccga ccgggaccgg ctgtatgcgc ttttcctcga
     4981 ggagagattc cagaccctga aacagggcaa agtttacaaa ttcgtggatt tcgcaccttc
     5041 attgccgcta tccagaaagc tgcggacgtg ctcctcactc gattaccgca gcgccgacct
     5101 gcaccgcaaa accgccgatg atcgggtcga catcactgac atggcgcttt atgcaaacga
     5161 ctcgattgac atgtttctct gttctcacat tttggaacat gtgcctgacg accaaaaagc
     5221 catgcgcgag ctgcaccgaa ttttaaaacc tggtggattc ggcatcgtca tggtgcccat
     5281 catcactgtg ctgcaagaca cccatgaaga tacctccatc acctcgccgg agggccgctg
     5341 gaaatatttc ggccaggacg accacctgag acaatattcc cgcgcgggag aaaacggctt
     5401 ggtgaccaga ctgcaaaaag cgggcttcac agtgcatctt tacgccgtgg agcactttgg
     5461 cgcagccgct ttcgacaaac acggcatagc tcgcaattcg gttctatacg tggttgaaaa
     5521 acacgccgcc gaataaaagg gcggctgttt aggaaggtaa aatctgctct tgattgccca
     5581 ctctgcgccg ctgaagtttg gcaggcaaat cctggttgag atagaaacaa tttgcctttc
     5641 tcactggccc gccggggcca gtcccggcat ctctaccgca tgcccggaaa catgcctttc
     5701 atgcccctca tcatctttcc catcccgccc tttgatatca ttttcatcat cttctgcact
     5761 tgctcgaact ggttgagcag gcgattcact tcctgcactg tcaccccggc gccggcggca
     5821 atgcgtcgct tgcgcgaggc cttgaggatt tccggtttgg cccgctcttg cggggtcatg
     5881 gaattgatga tgccctcgac gcggtgaatc gttttttcat ccatctgcgg gttttgcgcc
     5941 atttgcccca tttccccggg cagcttgtcg acgagcttgg ccaacccccc cattttccgc
     6001 atctgctgga tttgcgcctt gaagtcttcc aagtcaaaac ccttgccctg tttgactttc
     6061 tgcgcaagtt ttttcgcttc ttcctgatcc actccacgct gcacttcctc aacgagcgag
     6121 agcacatcgc ccatgccgag aatgcgcgaa gccatgcgct cgggatggaa gggttccaat
     6181 ccatccagct tttcgccgac gccggaaaac ttgatgggtt tgccggtgac aaagcgcacc
     6241 gaaagcgccg cgccgccgcg cgcatcgccg tcgagtttgg aaagcaccac gcccgtgagc
     6301 ggcagcgcgt cgttgaacgc cttggcggta ttcactgcgt cctgtccctg catcgcgtcc
     6361 accacgaaca gggtttcaat cggcttgagc gcggcatgca gttcgccgat ttcgcgcatc
     6421 atggtctcat caatcgcgag gcggcccgcg gtgtcgacga gcagcacgtc atgaaaatgt
     6481 ttgcgcgcaa aatcgagcgc gctttgcgca atcatggccg gcctgcttcc cgcctcgacc
     6541 gggaagaaat ccacattgag ttgctttgcc agcgtgcgca actgctcaat cgccgccggg
     6601 cggtacacgt cgcaactcgt gagcagcact tttttctttt gcgcctggag aatccgggcg
     6661 agcttgccgg cagtggtggt cttgcccgcg ccttgcaggc ccgctagcag aatcactgcg
     6721 ggcggtgcgg tggcgaggtc aagcccggca tgggaactcc ccatgagctg ggcaagctca
     6781 tgatgcacca ctccgatcgc ggcctggccg ggcgtgaggc ttcccaacac ttcctggccg
     6841 agtgcctttt cttttatgcg gttgatgaat tccttcacca ccggcagcgc cacatcggct
     6901 tccaacagtg ccatgcgcac ttcgcgcagc gcatcctgaa tattggtttc ggtaagccgc
     6961 gcctgaccgc gcagggtttt gatgacgccg gaaagtcgtt gcgcaagatg gtcgagcatg
     7021 gtgttggcgc cggttaatgc ctaagagatt cagttggtgt agactggaaa aagaaatacc
     7081 aatcttttca gtacatgccg gccattttac cttatctgat taccgccctg ctttacgccc
     7141 tgctgggttt tcatttctgg cgcacacgct ggcagcccgc cgcgcccgaa tccggcggca
     7201 ttgccgcgcc gatgcaatgg gaaagattcg cgattctcgt gccgtttgtt ctgcacagcg
     7261 cgctgctcta tcactccact ttcactggtg ccggcgtgaa cctcggcgta ggcaatgcgc
     7321 tgtctgcaat attatggctg gcggtgctgc tgtactggct ggtgagcttc ttttacaact
     7381 tcgaaggcct gcaggcgtta gtgttgcccc tggccgcggc gtgcgtgctc gtccctctac
     7441 tcctgcccac gacacacatg ctttccaaca ccgggcttcc ggcattccat gtgcatttcc
     7501 tgctgtccat gctcgcctac ggcctgctca ccatcgccgc attgcacgcg gtgctgatga
     7561 tgctggtgga acgccgtctg catcttggcg ccttgccggc tatgctcaac agcctgccgc
     7621 cgcttctcac catggagacg ctgctcttcc gtatcatcaa tgtggggttc gtccttttga
     7681 cgctagtgtt gatcagcggc atcctgtttt cggaagagct ctttcataag ccggcaccgt
     7741 ttacgcacaa gacattattt gccttcattt cctggggaat tttcgctgcg cttttgggcg
     7801 gcaggaaaat ctatggctgg cgcgggcgca ctgccacgcg ctggacgctt gccggcttta
     7861 tcgcgctgat cctggcctat attggcagca agtttgtgct ggaagtgatt ctgcaccgtt
     7921 gatgttttcg cgcggcggcg cgacttgaca aaaagtgagc gcgcaactga tactcaattc
     7981 gtctccttac tcgatcgtta aatccctttg gatgaaattc ccataagcgc attatttgct
     8041 gcgctcggct tgctccttat tatctccgcg tttttctcca tctccgaaac cagcatgatg
     8101 gcgctcaacc gctaccggtt gcggcacctg gtgcaagtcg gcagcagcgg tgccaagctc
     8161 acgtcacagc tgctcgcgca aaccgacaag ctgcttggcg tgattctgct cggcaacacc
     8221 ttactcaacg ccgcagccac caccttgagc accatcatcg ccgtgcagct ttttggtgat
     8281 agggaaatcg tgctgggctt gagcaccgtg tgcatcacct tcatgctgct ggtgttcagc
     8341 gaaatcacgc cgaaaatcat cggcgcctcc cagcccgagc gcatcgcgct cccggcgagc
     8401 tatctgctca cgccgctgct caagctgtct taccccgcgg tgtggatcgt taaccttttc
     8461 gccaaagcct tgctttggct gctgcgattc aaacccgccg cggaggcgca caaactcagc
     8521 cttgaagaac tgcgcacgct ggtgctggaa gcaagccatt tcattccgca aaaacaccaa
     8581 agcatcctgt tgaacctgtt agagctggaa aacatcacgg tgaacgacgt gatgacgccg
     8641 cgcaaccaca tcgaggctgt cgacatcgaa gcaccgcagg aggtgatacg ccgccagctc
     8701 gccacctgct atcacacccg gctgccggtg taccagagtc aat
//
LOCUS       DATIOW010000110         5481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_458371, whole genome shotgun sequence.
ACCESSION   DATIOW010000110 DATIOW010000000
VERSION     DATIOW010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458371"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VLL03_03595"
     CDS             complement(<1..812)
                     /locus_tag="VLL03_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSS46482.1"
                     /translation="MPFDSLGLSAEILRAIAEQGYVEPTPVQVQVIPSILSGLDVMGA
                     AQTGTGKTAGFTWPLLERLKIHASTSASPARHPVRALIITPTRELAMQVAESVAVYGK
                     YVALKSTVVYGGININPQIKELQSGVEILVATPGRLLDHVQQKTVNLSRVEILVLDEA
                     DRMLDMGFLPDIKRILALLPAKRQNLLFSATFSDEIKKLADQLLNQPVLIEVGRRNAA
                     VETVTHVVHPVDHNRKRALLAHIIKQQNLRQVLVFTRTKHGANRLAHQLQRDG"
     gene            complement(974..1513)
                     /locus_tag="VLL03_03600"
     CDS             complement(974..1513)
                     /locus_tag="VLL03_03600"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HSS46483.1"
                     /translation="MLKLIFLLAAVAAGALFWISVVRAGNAPNIGDAAPGFSLLDQNG
                     AVRNLRDFGGKWLVLYFYPKDDTPGCTQQACTFRDDLHQLTAMGAEVVGVSVDDSTSH
                     AEFARKYHLPFPLLADTKGEVAERYGSIYNAGFFKLAKRNTFLINPQGKIARVYLKAD
                     TSRNSKEVIADLKMLTAGK"
     gene            complement(1514..2698)
                     /locus_tag="VLL03_03605"
     CDS             complement(1514..2698)
                     /locus_tag="VLL03_03605"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSS46484.1"
                     /translation="MQQRLPRTVVILGLVSFFNDFASDIVIPLIPILLVTVLSSGPVA
                     LGLIEGVADAVASFLKLWSGRHSDRLSGRRKGLTLFGYTLSNLARPFLGLAGNWPLVL
                     VLRSLDRVGKGLRSAPRDALVVDVTPPSMLGYAFGFHRALDNGGAVMGSLAAAAVLAY
                     STFTLPQVILLSAVPGFVAVLFLVAGVKEKPAPAVQPRAELAPLSWRGLSLPMRRYLA
                     VLGLFTFARNSETFILLLGFQLRISVVELLLLWAALNFAKAATSTWGGRLADKLGRGQ
                     LMLISWTAFAMSFLLLGKVSGSAGLWGVTLFYGLFTGLSEGAERALIGDFSSSNERGT
                     AFGWYHLIIGFAAIPAGLVFGVLWQYQSAALAFFFASAIAASSALLLRGWVRMPLVQK
                     KS"
     gene            2810..4690
                     /locus_tag="VLL03_03610"
     CDS             2810..4690
                     /locus_tag="VLL03_03610"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HSS46485.1"
                     /translation="MGTMSAGGSRPLLIYDGDCGFCTYWVRYWQKLTQDRVDYAPYQE
                     VAAQYPHIPVSEFQRAVQYIAPDGSTARAAEASFLTLSHASGKSFWLALYRRLPGFAA
                     LTELAYAFIASHRTAFYRLSLLLWGRDCAPPRFDSVSWLFLRAIGLIYLAAFVSFGVQ
                     ALGLIGSHGILPLPEFIEVVNQHFGVERYWLFPVLFWWGAGDFVIQAICWAGAALSLL
                     VVFNVLPRLSLLMLYALYLSLFYAGQDFMTFQWDLLLLETGFLALLLSTGSRLGLWLL
                     RWLLFRFIFFSGAVKLLSGDPTWADLSALSYYFQTQPLPTPLAWYVHQLSPSVLAFAT
                     SMTFFIELVLPFLIFCPRRLRFVAAFSFLLLQVTILLTGNYNFFNLLTMALCLTLFDD
                     AALNHLLPQNLARSLQQRVRGMQPGKAALFVVASFALLIVLVSGVQLHVMFGGRVPAL
                     AIWINNSIAPFRIVNTYGLFAVMTTERPEIIIEGSNDGTHWSEYAFKYKPGDVKRAPP
                     WNIPHQPRLDWQMWFAALESQSQHPWFERLLQRLLENSPEVIALLKTNPFPNKPPHYV
                     RALLYDYRYSSREEKALRSVWWQRRLAGLYYPAVELRQNSLSGREAPSNLMEAMMRPR
                     QP"
     gene            4754..4829
                     /locus_tag="VLL03_03615"
     tRNA            4754..4829
                     /locus_tag="VLL03_03615"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4787..4789,aa:Arg,seq:cct)
     gene            5189..>5481
                     /locus_tag="VLL03_03620"
     CDS             5189..>5481
                     /locus_tag="VLL03_03620"
                     /inference="COORDINATES: protein motif:HMM:NF041635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM3941 family protein"
                     /protein_id="HSS46486.1"
                     /translation="MAILLAGSLAFVAAGAWLVSEKPLIGYLCVILFGLCALVFAVQL
                     HPDSSYLTLSSEGFTFCALFRRTFVPWSQAGEFVVVNVRNHKMVGWNYAPEYQG"
BASE COUNT         1129 a         1630 c         1505 g         1217 t
ORIGIN      
        1 ccgtcgcgct gcaactgatg cgcgagacgg tttgcgccgt gcttggtgcg ggtgaacacc
       61 agcacctggc gcaaattctg ctgtttgatg atgtgcgcga gcagcgcgcg cttgcggttg
      121 tggtccaccg ggtgcaccac atgggtgacg gtctccaccg cggcgttgcg gcgtcccact
      181 tcaatcagca ccggctggtt gagaagctga tcggcgagct tcttgatttc gtcggagaat
      241 gttgccgaaa agagcaggtt ctggcgcttc gccggcagga gtgcgaggat tctttttata
      301 tccggcagga aacccatgtc gagcatgcgg tcggcctcat ctagcaccag gatttccact
      361 ctggaaagat tgaccgtctt ttgctgcaca tgatcgagca gccggccggg cgtcgccacc
      421 agaatctcca cgcccgattg cagttccttg atttgtggat tgatgttgat cccgccgtaa
      481 accaccgtgg acttgagcgc gacgtatttt ccgtagaccg ccacgctttc ggccacttgc
      541 atcgccagtt cccgcgtcgg cgtgataatc agcgcgcgca ccggatggcg cgccggcgaa
      601 gcactggtgc tggcgtggat tttgagccgc tccaaaagcg gccaggtgaa gcccgcggtc
      661 ttgccggtgc cggtctgcgc ggcgcccatc acatccaggc cggaaagtat tgatggaatc
      721 acctgcacct gcaccggcgt gggctcgaca taaccctgtt ccgcgatggc acggagaatt
      781 tcggctgaca ggccgagtga gtcaaaaggc attttcaatg ctcctaaatc ggcctacctg
      841 ggctttgcac ccgggaccgg tctaggcaga tgacaatgtg taagaggaat accgcgaggg
      901 tcggcggccc agaccggaca gttggaccgc actcgccgcg catcatactc cattcgcgcc
      961 gcttgtaaaa cgactatttt cctgcagtca gcattttcag gtctgcgatg acttctttgg
     1021 aattgcgcga cgtgtcggcc ttgagataaa ctctggcgat ttttccctgc ggattaatca
     1081 ggaaggtatt gcgcttggcc agtttgaaga agcctgcgtt ataaatcgag ccgtagcgct
     1141 ccgccacttc gcccttggtg tcggcgagca acggaaaagg cagatggtat ttcctggcga
     1201 attcggcgtg gctggtgctg tcatccacgc tcacgcctac cacttccgca cccatggcgg
     1261 tgagctggtg caaatcgtcg cggaaggtgc aggcctgctg ggtgcagccg ggggtgtcgt
     1321 ccttaggata gaaatacagc accagccatt tgccgccgaa atccctgaga ttacgcaccg
     1381 cgccattttg gtcaagaagg gaaaaacccg gcgcggcatc accgatattt ggcgcgttgc
     1441 cggcacgcac cacgctgatc caaaacagcg cgcccgccgc caccgccgcc aataaaaaaa
     1501 tcagtttcaa cattcaagac ttcttctgta ccagaggcat cctcacccag ccgcgcaaca
     1561 gcaacgcact cgaggcggca atggcgctgg cgaaaaaaaa cgccagcgcc gcgctctggt
     1621 actgccacag gacgccaaac accaagccgg cgggaatcgc cgcgaagccg ataatcaaat
     1681 gataccagcc aaacgcggtg ccacgttcat tggagctgga aaaatccccg ataagtgccc
     1741 gctccgctcc ttcgctcaaa ccggtgaaca agccatagaa aagagtcacc ccccacagcc
     1801 ccgcgcttcc cgacaccttg ccgagcagaa gaaagctcat ggcgaacgcg gtccagctta
     1861 tcaacatcaa ctggccgcgg cccaatttgt ccgcgagcct cccgccccaa gtggatgtcg
     1921 cggccttggc aaaattgagc gccgcccaca acagcagcag ttccaccacg cttattcgca
     1981 actggaaacc gagcagcagg ataaaagttt cggaattacg ggcaaaggta aacaatccta
     2041 gcaccgccag atagcgccgc atgggtaacg acaagccgcg ccaggaaagc ggcgcaagtt
     2101 ctgcacgcgg ctgtactgcg ggagcgggtt tttccttgac tcccgctacc aggaacaaca
     2161 cggcaacgaa tcccggaact gcggaaagca aaatcacctg cggcaaagta aacgtggaat
     2221 aggcgagcac cgccgcggcg gcgaggctgc ccatcaccgc tccgccgtta tccagcgcgc
     2281 gatgaaaacc gaaggcgtaa cccagcatgg acggcggggt gacatccacc accagcgcat
     2341 cgcgcggcgc gctacgcagg cctttgccaa ctcgatccag actgcgcaat accagcacca
     2401 gcggccagtt ccccgccagc cccaggaacg gccgcgccag attggagagc gtatagccaa
     2461 acagcgtcag ccccttgcgc ctgccgctta agcgatcgga atggcgcccc gaccacagct
     2521 tcaaaaaact cgcaacggca tcggcaacac cttcaattaa accgagcgcg acggggccgg
     2581 aagaaagcac cgtcaccagc aggattggaa tcaacggaat gacgatgtcc gacgcaaagt
     2641 cgttgaagaa actaaccagg ccgagaatga ccacggtgcg cggcaatcgc tgctgcaatg
     2701 tttttcctgt gcaaaatgac aaacgggtgg aagcattgta agccaaacgc cccgccacgg
     2761 ctgttattcg cggttccctt ctttttccct tcaggcataa tctggtttta tgggaaccat
     2821 gagcgcaggc ggctctcggc cgctcttgat ctatgacggc gattgcggtt tctgcaccta
     2881 ctgggtgcgt tattggcaaa agcttactca agaccgggtg gactatgcgc cctatcagga
     2941 agtcgccgct cagtacccgc acattcctgt ttccgagttt caacgcgcgg tgcagtacat
     3001 cgcgcccgat gggagcaccg ctcgcgccgc cgaagcctcc tttctcacgc tttcccatgc
     3061 aagcggcaaa agtttctggc tggcgttata ccgccggctg ccgggtttcg ccgcgctgac
     3121 ggagctcgct tacgcgttca tcgcttcgca ccgcacggcg ttttatcgct tgagcctgtt
     3181 gttgtggggg cgcgactgcg ctccgccgcg atttgattct gtttcctggc tgtttttgcg
     3241 cgcgatcggc ctgatttatc tcgccgcgtt tgtttctttt ggggtgcagg cattggggtt
     3301 gatcggcagc cacggcattc tgccgcttcc agaatttatc gaggtcgtga accagcattt
     3361 cggggttgag cgctactggc ttttccccgt gctgttctgg tggggtgcgg gcgatttcgt
     3421 cattcaagcc atctgctggg ccggcgcggc gctatcgctg ctggtggtgt tcaacgtcct
     3481 gccgcgcttg agtctgctta tgctttatgc gctatatctc tcgctgttct acgccgggca
     3541 ggatttcatg acgtttcaat gggatttgct gctgctggag accggatttc ttgcgcttct
     3601 gctgagcacg ggctcaaggc ttggcctttg gctgttgcgc tggctgttgt tccgcttcat
     3661 atttttttca ggtgcggtaa agcttctgag cggcgatccc acttgggcgg acctctcggc
     3721 actttcctac tactttcaaa cccagccgct gcccacgcca ctagcttggt atgtccatca
     3781 gctttcgcca agcgtgcttg ctttcgccac cagcatgacg tttttcatcg agctcgtgct
     3841 gccgttcctg attttctgcc caaggcggtt gcgctttgtt gcggctttta gctttctact
     3901 gctgcaagtc accattctcc tcaccggcaa ttacaatttc ttcaatctcc tcaccatggc
     3961 gctatgcctc accttgttcg acgacgcagc cctcaaccat ctgttgccac aaaacctggc
     4021 ccgctcactt cagcaacgtg tgcgcggcat gcagccggga aaagccgctt tgtttgtcgt
     4081 tgcttcgttt gccctgttga ttgtgctggt gagcggcgtg caacttcatg tgatgtttgg
     4141 cggccgcgtg cccgcactag ctatatggat caacaacagc atcgcgccat ttcgcatcgt
     4201 caacacttac ggcttgttcg cggtgatgac caccgagcgg ccggaaatca ttatcgaagg
     4261 ctcgaacgac ggtacccact ggagcgaata tgcgttcaag tacaagccgg gcgatgtgaa
     4321 acgggcgccg ccgtggaata tcccgcacca gccgcggctg gattggcaga tgtggtttgc
     4381 ggcgctggaa agccagtcgc agcatccctg gttcgagcgt ttgctgcagc ggctcctgga
     4441 aaactccccg gaagtgatcg cgctcctcaa gaccaatccg tttccaaaca aaccgcctca
     4501 ttacgtgcgc gcgctgcttt acgactaccg gtattcgagc agagaagaaa aagctctgag
     4561 aagcgtgtgg tggcagcgca ggcttgcggg tctttattat cccgcagtgg aattaaggca
     4621 aaacagcttg agcggccgtg aagccccttc caatctcatg gaagcaatga tgcggccacg
     4681 ccagccttaa gcctcaaccg ccgttgccgt acgagtgata agtggaggca atataggcta
     4741 gaatgcgcgc gtcgccccga tagctcagtg gatagagcag tcccctccta agggacaggt
     4801 cgcacgttcg attcgtgttc ggggcgccaa ccagacccac tgatcccccc atggatctat
     4861 tctcgcccgc tggcaccgaa aactgaccgg gtctatactc aacccaacag gaataagggt
     4921 gcatccctat cccaggaaag gatcatgtac ccgtaagccc cagtgttaca agcggggact
     4981 tgcaaaggtg tatttgatgt tgtcatttcg ggtgtgtaca gaaatctgat tcctaagata
     5041 tcaagtggtt aacattctta tttccggatt agactccaaa attagtgtaa ttaaaggcac
     5101 tttttaagac gtctatttct agttagagcc ttgtatgaac gccgccacca caccggtccc
     5161 agcaatactc cggactagtc gcatcaagat ggcgattctt ctggctggct ctctcgcttt
     5221 tgtagccgct ggtgcttggc ttgtctccga gaaacctctc atcggatacc tgtgcgtcat
     5281 cctcttcggg ttatgcgcgc ttgtgtttgc agttcagctg catcctgaca gctcctactt
     5341 aacgctctca tccgaaggct tcaccttctg tgcgctgttt cgccgcacct tcgtcccgtg
     5401 gtctcaggcg ggcgaattcg tggtcgtaaa cgtccgaaat cacaagatgg tcggctggaa
     5461 ctacgctccc gaatatcaag g
//
LOCUS       DATIOW010000111         9040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1069984, whole genome shotgun sequence.
ACCESSION   DATIOW010000111 DATIOW010000000
VERSION     DATIOW010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9040
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069984"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VLL03_03625"
     CDS             <1..620
                     /locus_tag="VLL03_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSS46487.1"
                     /translation="LTKGKILPHRLIYISTSGVYGHCGGETVPETRPLKPQTERARRR
                     LDAERRVRDWGRRSGVRVSILRVPGIYAEDRLPLERLKSGTPALLPDQDAYTNHIHAD
                     DLARIVIAALRLGRPGRVYNTSDDSELKMGDYFDLVADRFGLPRPPRIPQAEAGQKLS
                     ETLLSFMQESRRLANRRMKRELKVKLHYPVVADGIAAAQAHDSRC"
     gene            703..1209
                     /locus_tag="VLL03_03630"
     CDS             703..1209
                     /locus_tag="VLL03_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46488.1"
                     /translation="MLLALLAGCGVAPALPDNPAATAGEPLPIRLAVEMTPEQFAQWG
                     SQFYYYYEIYYPEGQLVRNAALDAFAPLVSQVLPREQMPAPDVIVKVSGSGFFNPLMR
                     VFYAKVSVTAYSASGEELGTFKSSSDVGGIVSGYERAFQRAYLVAFQDIGRKLLESGA
                     LSSVERQN"
     gene            1294..1629
                     /gene="cutA"
                     /locus_tag="VLL03_03635"
     CDS             1294..1629
                     /gene="cutA"
                     /locus_tag="VLL03_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HSS46489.1"
                     /translation="MKAEAILVITNLPDRESALKLANKLVEARLAACINVMAECTSIY
                     RWKGKIETAPEVPVFIKTMKTHFPQVEKLVKQHHPYELPELIAVPITTGLAAYLEWVA
                     AETLPVKNN"
     gene            1645..3462
                     /gene="dsbD"
                     /locus_tag="VLL03_03640"
     CDS             1645..3462
                     /gene="dsbD"
                     /locus_tag="VLL03_03640"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HSS46490.1"
                     /translation="MRLLFFLLMLFSLPACAAEPELLEPEKAFRFSAEMLDPGNIEVR
                     YQIAEGYYLYRERFRFEVQPAGFSLGKPQIPAGEIHQDQFFGRVETYRRDLKIKLPLK
                     QALGPSQTLTLKAVSQGCADAGVCYTPLEQSAHLSLVAATSQLPAAPATIASLQGLKA
                     AASPSPLLSSNDPAANDESSQIANLLRVGSFWFIVASFFGFGLLLAFTPCVFPMIPIL
                     SGIIVGQKQTMTKARGLVLSGAYVLGVAITYAIAGVAAGLSGTLLSNALQNAWVLGAF
                     AALFVALALSMFGFYELQFPASVQGRLNDAANRIKAGNLAGAFVMGGLSALIVGPCVA
                     APLAGALLYISQSRDVVLGGSSLFAMALGMGVPLLAVGISAGTLLPKAGPWMDAVKRF
                     FGVLLLGVAIWIISPLISLQVHMALWATLLIVWAVYLKAVDPLPAEALGYARLGKGIG
                     IIALVAGIALLVGVFSGERDILRPLARLNGPIHLMQGLKFQRVKSNAELDQAVAQAGG
                     KYVMYDFYADWCVSCKEMERFTFTHPAVQAKLKDVVLLQADVTANTAEDAAMLKRFGL
                     FGPPGTLFFDKQGNEIKGVRVVGYQSPEKFLATLDAALK"
     gene            3463..3987
                     /locus_tag="VLL03_03645"
     CDS             3463..3987
                     /locus_tag="VLL03_03645"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSS46491.1"
                     /translation="MGKRALLFFAVAVLALGAGIFFGWQAKAPAVAEAGAAQALFAAN
                     IPDLNGQNQGLAQWRGKVLVVNFWATWCPPCREEIPQFVKVQDKYRDRGLVFVGVALD
                     KKDAVEAYIKEVGINYPVLLGDLDAMELSRKAGNRAGALPFTAILDRNGKIVSTQLGG
                     LTQAKLDAIITPLL"
     gene            4082..4513
                     /gene="aroQ"
                     /locus_tag="VLL03_03650"
     CDS             4082..4513
                     /gene="aroQ"
                     /locus_tag="VLL03_03650"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HSS46492.1"
                     /translation="MKNILVLHGPNLNLLGSREPEHYGHDTLEAIDVRLKERAKQAGA
                     RLACFQSNAENTLVERIQQASKEGVEFIIINPAAYTHTSVAIRDALAAVKIPFIEVHL
                     SNIHAREPFRHHSYFSDLAVGVISGLGARSYELALEFALQT"
     gene            4589..5053
                     /gene="accB"
                     /locus_tag="VLL03_03655"
     CDS             4589..5053
                     /gene="accB"
                     /locus_tag="VLL03_03655"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HSS46493.1"
                     /translation="MDLRKLKKLIDLVEKSGIAELEITEGEEKVRISRVSQSSVQATV
                     TPVAQPASATVVQPATPAVTAAAGTPAAPAEPEGHVVKSPMVGTFYRSPSPGAKAFVE
                     VGQRVNAGDTLCIIEAMKLLNEIETDRAGVIKEILAENGQPVEYGEPLFVIG"
     gene            5167..6522
                     /gene="accC"
                     /locus_tag="VLL03_03660"
     CDS             5167..6522
                     /gene="accC"
                     /locus_tag="VLL03_03660"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HSS46494.1"
                     /translation="MFEKILIANRGEIALRIQRACREMGIKTVAVHSEVDAEAKYVKL
                     ADESVCIGPAPSDQSYLNIPAIISAAEVTDAEAVHPGYGFLSESADFAERVEKSGFVF
                     IGPRPETIRLMGDKVSAKVAMKKAGVPCVPGSDGSLPDSPEQIVKIARTVGYPVIIKA
                     AGGGGGRGMRVVHTEAALMNAVAMTRSEAQAAFGNPTVYMEKFLENPRHIEIQVLADE
                     YRNAIFLGDRDCSMQRRHQKIIEEAPAPNLHPKQRGKIGERCADACRKISYRGAGTFE
                     FLYENGEFYFIEMNTRLQVEHTVTEMVTGIDLVQGQIRIAAGEKLRIRQKDVALKGHA
                     IECRINAEDPYKFVPSPGRITSYHTPGGPGIRMDSHVYQNYFVPPYYDSMIGKLIAYG
                     DTRAQAIARMRIALSEMIVEGIKTNIPLHQELMLDAAFLRGGTNIHYLEQKLAGYKKN
                     Q"
     gene            6546..7433
                     /gene="prmA"
                     /locus_tag="VLL03_03665"
     CDS             6546..7433
                     /gene="prmA"
                     /locus_tag="VLL03_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HSS46495.1"
                     /translation="MNWLSLTLEANAEQAEILSESLLQLGALSVDISDARAGTPDEQP
                     VFGEPGESSPTLWQSSRVNGLFPAGVDIAAIVKTASLTAHLVHPKFTVADAPDRDWVR
                     LVRLQFAPMQISPRLWVVPTWHAAPDAHALNLILDPGLGFGTGSHPTTRMCLNWLDAH
                     LKGGEQVLDYGCGSGILALAAKRLGAQRAVGVDCDAQALDSSRANARQNKVDAEFYTP
                     NELPPFEADVVLANILANPLKLLAPLLAQATRRGGAIVLSGILRGQACEVEEAYLQWF
                     GMRKTDEDSGWVCLAGTKR"
     gene            7439..8224
                     /locus_tag="VLL03_03670"
     CDS             7439..8224
                     /locus_tag="VLL03_03670"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HSS46496.1"
                     /translation="MSMVTTCPSCHTTFRVRHKDLQAGEGRVRCGQCTEVFNALDSLV
                     TLDDAPGAVRETVLQARSMPQKPSAPETIPNLQTKVSGKQAEEPLKFTDEALEKAERS
                     PAKTIAWSAAALLLLLLLAAQVAYFFRVPIAAYYPETKPFLQRYCELLQCAVDLPRYA
                     EWISIEASDLQADPQRPNLIILTATLRNRATFVQAYPALELTFTNTQAQMVARRVFTP
                     ADYLASGVDASRGMEANNEFTTKLFIDSGDLEPSGYRLFLFYQ"
     gene            8332..8832
                     /gene="tpx"
                     /locus_tag="VLL03_03675"
     CDS             8332..8832
                     /gene="tpx"
                     /locus_tag="VLL03_03675"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HSS46497.1"
                     /translation="MAKVTLAGNPITVAGHFPAVGQMAPDFKLVNKDLKDVSLKDFAG
                     KRKVLNIVPSLDTPTCQKSTRRFNEEANKLNNTVVLVISADLPFAQARFCGAEGLNNV
                     MTLSTMRGAEFMKNYGVAITDSPLGGVTARAVVVLDGDDKVLYTEMVPEIKNEPNYDA
                     ALKALR"
     gene            8854..9006
                     /locus_tag="VLL03_03680"
     CDS             8854..9006
                     /locus_tag="VLL03_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46498.1"
                     /translation="MDCRRLNLIALFAPGKAKPLAADITPDASEIFIPVPDIKAGRLS
                     RLQTQH"
BASE COUNT         1925 a         2575 c         2633 g         1907 t
ORIGIN      
        1 cgctgacaaa gggaaaaatt ttaccacatc gcctcatcta catcagtacc agcggtgtgt
       61 atggccattg cggcggcgaa accgttcccg aaacgcggcc gctcaaaccc cagaccgagc
      121 gcgccaggcg ccgcctcgac gccgagcgcc gggtccgcga ttggggaagg cgcagcggcg
      181 tgcgcgtgag cattttgcgc gtgccgggga tttacgccga agaccgcttg ccgttggagc
      241 gtttaaaaag cggaaccccg gcactgctcc ccgatcagga cgcctacacc aatcacatcc
      301 acgccgacga cctggcgcgc attgtaattg ccgcgctgcg cctggggcga ccgggaaggg
      361 tgtacaatac cagcgacgat tcggaactca agatgggcga ttacttcgat ttggtcgccg
      421 accgcttcgg cttgccgcgc ccgccgcgca ttccgcaagc cgaagcggga caaaagctgt
      481 ccgaaaccct gctttccttc atgcaggaat caaggcggct tgccaaccgg cgcatgaagc
      541 gcgagcttaa ggtgaaattg cactatccag tagtcgctga tggcatcgcc gcggcacaag
      601 cccacgacag tcggtgctga acttaattgg cgctgtttcg tcttaatgga tattgcatca
      661 gtgccgtgta tgacacaccc tgtcttaaaa acgttccccg ccatgctgct cgccttgctg
      721 gcaggttgtg gtgtcgcgcc cgccctgccg gataaccccg ccgccacggc cggcgaaccg
      781 ttacccatcc gccttgccgt ggaaatgacg ccggaacagt tcgcgcaatg ggggtcacaa
      841 ttttactatt actatgagat ctactatcca gaagggcagc tggtgcgcaa cgcagcactt
      901 gacgctttcg cgcccttggt ctcccaggtt ttgccgcgcg agcaaatgcc ggcgccggac
      961 gtgatcgtca aagtaagcgg cagcggtttt ttcaatccct tgatgagagt tttctatgcc
     1021 aaggtgagcg tcaccgccta ctcggccagc ggggaagaac tgggcacgtt caagtcgagt
     1081 tccgatgtcg gcggcattgt cagcggttat gagcgcgcgt ttcaaagggc ataccttgtc
     1141 gcgtttcaag acatcggcag aaaactcctc gaaagcggag cattaagctc cgtcgaacgt
     1201 caaaactagt ccgctccaca cggcccgcga cacgggcgcc tctgaatggt ccggaaccgc
     1261 gtagaatagg cgcaaactca aacccggatt accatgaaag ccgaggcgat tctcgtcatc
     1321 accaatcttc ccgatcgcga gtccgctttg aaactcgcca acaaactcgt ggaagcgcgg
     1381 cttgcggcat gcatcaatgt catggccgaa tgcacatcga tttaccgttg gaagggaaaa
     1441 atcgaaaccg cgcccgaagt gccggttttc atcaaaacca tgaaaacgca ttttccgcaa
     1501 gtggaaaagc tggtaaaaca gcaccaccct tacgaacttc ccgagttgat tgctgtccct
     1561 ataaccaccg gattggcagc ctatctggaa tgggtcgcgg cggagacgct gcccgtcaaa
     1621 aacaactagt tttattcccg taacatgcgt ctgctgttct ttttgctgat gctgttttcc
     1681 ctgcccgcgt gcgcggcgga gccggaattg ctcgagccgg aaaaggcatt ccgtttttcg
     1741 gcggaaatgc tcgatcccgg caatatcgaa gtccgctatc aaatcgccga aggttattac
     1801 ctgtatcgcg agcgtttccg gtttgaagtg cagccggcgg gattttccct gggcaagccg
     1861 cagataccgg cgggcgaaat acatcaggac cagtttttcg ggcgcgtcga aacctatcgg
     1921 cgtgacctca aaatcaagtt gccgctcaag caagcgctgg ggccaagcca gactttgact
     1981 ctgaaagcgg tgtcgcaagg ttgcgccgat gcgggggtgt gctacacccc gcttgagcag
     2041 agcgcgcatc tgagtctggt tgccgcaacc agccagttgc ctgctgctcc cgccacaatc
     2101 gccagcctgc agggcctgaa agccgccgca tcgcccagcc cgttgcttag ttccaatgac
     2161 ccggccgcta acgatgaatc atcgcaaatc gccaatttac tcagggtcgg aagcttttgg
     2221 tttatcgtcg cgagcttttt tggctttggc ttgctgctgg cattcacgcc ttgcgtgttt
     2281 ccgatgatcc cgattctctc cggcatcatc gttggacaaa aacaaacaat gaccaaggcc
     2341 cgcgggctgg tgttgtccgg agcctatgtg ctgggcgtgg cgattaccta cgccatcgcc
     2401 ggcgtagcgg cgggcttgag cggaacactg ctttccaacg cgctgcaaaa cgcctgggtg
     2461 ctcggtgcgt ttgccgcgct tttcgtggct cttgcgctgt ccatgttcgg tttttatgag
     2521 ctgcaattcc ccgcctccgt tcaagggaga ttgaatgatg cggcgaatcg catcaaagct
     2581 ggcaatctcg ccggtgcgtt cgtcatgggc gggctttccg cgctcatcgt cgggccttgc
     2641 gtggccgcgc cgcttgcagg cgcgctgctt tatatcagcc agtcgcgcga tgttgttttg
     2701 ggtggctcga gtttgtttgc catggcgctg ggcatgggcg tgccactcct ggcggtgggc
     2761 atttccgcag gtacgctctt gcctaaggcg ggtccgtgga tggatgcggt gaaacggttt
     2821 ttcggggtgc tgctgctcgg tgtggcgatc tggatcattt caccgttaat ttcgctgcaa
     2881 gtacacatgg ctttgtgggc gacgctgctg atcgtctggg cggtgtacct gaaagccgtc
     2941 gatccgctac ctgctgaagc gttgggctac gcgcgactgg ggaaaggaat cggtatcatc
     3001 gccctggtcg caggaattgc acttttagtt ggcgtgttct ctggcgagcg cgatatattg
     3061 cggccactgg cccggcttaa cggtcccatc cacctcatgc agggcctcaa attccaacgc
     3121 gtcaaaagca atgcggaact tgatcaggcg gtagcgcaag ccggaggcaa atatgtcatg
     3181 tacgattttt acgctgactg gtgcgtgtca tgcaaggaaa tggagcgctt cacttttacc
     3241 caccccgcgg tgcaagccaa actcaaagat gtcgttttgc tgcaggccga tgtgacggcg
     3301 aataccgcgg aggatgctgc catgttgaaa cgctttgggt tgttcggtcc gccgggaacc
     3361 ctgtttttcg ataagcaggg aaatgagatt aagggcgtgc gggtggttgg ctatcagagc
     3421 ccggaaaaat tcctcgctac tctcgatgcg gccttaaaat aagtgggcaa gcgcgcgctc
     3481 ctcttttttg ctgttgcggt tctggctttg ggcgccggca tatttttcgg ctggcaagcc
     3541 aaggcgccgg cagtggcgga agcaggtgcc gcccaggcgc tgttcgccgc caatattcca
     3601 gacctcaacg ggcaaaatca gggtctggcg cagtggcggg gcaaggtgct ggtggtcaat
     3661 ttttgggcga cctggtgccc gccatgccgt gaggaaatcc cccagttcgt gaaggttcaa
     3721 gacaaatacc gcgaccgtgg actggttttc gtcggggtgg ccctggacaa aaaggacgca
     3781 gtggaagcct atattaagga agtaggcatt aactacccgg ttttgctggg tgacctcgat
     3841 gccatggagt tgtcgcgcaa ggccggcaat cgtgctgggg cactgccatt taccgctatt
     3901 cttgaccgta atggcaagat cgtcagcacc cagcttggtg gattgacgca ggctaagctc
     3961 gacgccataa tcacccctct gctctaaaaa cccactattt cctccaagca ttttgctgta
     4021 atttaggcga agttcagtga aaaagtggac aatttggccg aaattgtgca aactttggtg
     4081 tatgaaaaat atcctggttt tgcacggccc caatttaaat ctgctggggt cccgtgagcc
     4141 ggaacattat ggtcacgata ccttggaggc gattgatgtc cgccttaagg aacgcgctaa
     4201 acaggcgggc gctaggctcg cctgtttcca gagcaacgcc gagaacaccc tggtggaacg
     4261 catccagcag gcaagcaagg aaggggtgga gttcatcatc atcaacccgg cggcgtatac
     4321 ccacaccagc gttgccatcc gcgacgcgct cgccgcagtg aaaatccctt ttatcgaagt
     4381 tcatctttca aatattcatg cccgtgaacc cttccgccat cattcctatt tttccgacct
     4441 ggcggtagga gtgattagcg gtctcggtgc caggagttac gagctggcgc ttgagttcgc
     4501 cctgcaaaca tgagtctctg aagccgtcct gccgcgtcaa cgcggttctt ctcaagcttt
     4561 ggggctcaca cattggtaga ggccagctat ggatttaaga aaacttaaaa agctgatcga
     4621 tctggtggag aagtcgggga ttgccgaact ggaaatcacc gaaggcgagg aaaaagttcg
     4681 catcagccgg gtaagccagt cgtcggtgca ggccaccgtg actcccgttg cgcagccggc
     4741 aagtgcaacc gtcgtccaac ccgcaacccc tgcagtaacc gcagctgccg gtacgccggc
     4801 agcgcccgcc gaacccgagg ggcatgtggt gaaatcgccg atggtcggca ccttctaccg
     4861 ctcgcccagc cccggcgcca aagcgtttgt cgaagtcggg cagcgggtca atgccggcga
     4921 cacgctgtgc atcatcgagg cgatgaaact gcttaacgaa atcgaaacgg accgcgccgg
     4981 ggtgatcaag gaaatcctgg cagagaacgg ccagccggtg gaatacggcg aaccgctgtt
     5041 cgttattggg taaggagtaa agagtgagag gcgaggagtt aactgctcag gagcttcagt
     5101 cttattccgc gcttattttt gcttttacac ctcaagcctc actttttact cctcccggtt
     5161 tttcacatgt ttgaaaaaat tctgattgcc aatcgcggtg agatcgccct gcgcatccag
     5221 cgcgcctgcc gcgaaatggg catcaagacc gtggccgtgc attccgaggt ggatgccgaa
     5281 gccaagtatg tgaagcttgc tgacgaatcg gtgtgcatcg gcccggcgcc atccgaccag
     5341 agctatctca acattcccgc catcatcagc gccgcggaag tgaccgacgc cgaggcggtt
     5401 catcccggtt atggcttcct ttctgaaagc gccgatttcg ccgagcgcgt ggagaaaagc
     5461 ggcttcgtct tcatcggccc gcgtccggaa acaattcgcc tgatgggaga caaagtgagc
     5521 gccaaggtgg cgatgaagaa agcgggagtg ccctgcgtgc cgggttcgga cggcagcctt
     5581 cccgattcac ccgagcaaat cgtgaaaatt gcgcgcaccg tcggctaccc cgtgatcatc
     5641 aaggcggcgg ggggcggcgg gggacgcggc atgcgcgtgg tgcatactga agcggccctg
     5701 atgaacgcag tggcgatgac gcgttccgaa gcgcaagccg ctttcggcaa tcccaccgtt
     5761 tatatggaga aattccttga aaatccgcgt cacatcgaaa ttcaggtgct cgccgacgag
     5821 tatcgcaatg ccattttcct gggagaccgc gattgctcga tgcagcgacg gcaccagaaa
     5881 atcatcgagg aggcgcccgc gccgaatttg catccgaagc agcgcggtaa aatcggtgag
     5941 cgctgtgccg atgcctgccg caagatcagc taccgcggcg ccggcacgtt tgaatttctc
     6001 tacgaaaacg gtgagtttta tttcatcgaa atgaacacgc gcctgcaggt cgagcacacg
     6061 gtcaccgaaa tggtgaccgg catcgacctg gtgcaggggc aaatccgcat tgcagcgggt
     6121 gaaaaactgc gcattcggca gaaggacgtg gcgctaaaag ggcacgccat cgagtgccgc
     6181 atcaacgccg aagacccata caaattcgtt ccgtcgccgg ggcggattac ctcctatcac
     6241 acgcccggcg gtccgggcat acgcatggat tcccacgtct accagaatta tttcgtgccg
     6301 ccgtattatg actcgatgat cggcaagctg attgcctatg gcgatacgcg cgcgcaggcg
     6361 attgcgcgca tgcgcatcgc actttcggaa atgatcgttg aaggcatcaa gaccaacatc
     6421 ccgctgcacc aggaactgat gctggacgcc gctttcctgc gcgggggaac caacattcat
     6481 tatctggaac agaaattggc cggatacaaa aagaatcaat gaaaactgcg gtcttggata
     6541 caacaatgaa ctggctgtcg cttactctcg aagcgaatgc ggaacaagcc gaaatcctga
     6601 gcgagtcgct gctgcaactg ggtgcgctgt ccgtggacat ttccgatgcc cgtgccggaa
     6661 ccccggatga gcaaccggtt ttcggcgaac cgggcgagtc gtccccgacc ctatggcagt
     6721 ctagccgggt aaacggcctg tttcccgccg gcgtggatat cgccgccatc gtcaaaaccg
     6781 catcgctcac agcgcatctc gttcacccca agtttactgt cgccgatgcg ccggaccggg
     6841 attgggtgcg tttggtgcgg ttgcagtttg cgccgatgca gatttcgccg cgcctgtggg
     6901 tggtgccgac ttggcatgct gcgcctgatg ctcatgcgct taatctcatt cttgaccccg
     6961 gtctggggtt tggcaccggc agccatccga ccacccgcat gtgcttaaac tggctggatg
     7021 cgcatctcaa gggcggggaa caggtgcttg actacggctg cggttccggg attcttgccc
     7081 ttgcggctaa aagactcggc gcgcaacgtg cagtgggcgt ggactgcgac gcgcaagcgc
     7141 tcgactcgag cagggccaat gcacggcaga ataaagtcga tgcagagttt tatactccaa
     7201 acgagttgcc gccattcgag gccgatgtag tactcgccaa catcctggcc aatccgttga
     7261 agctgctggc accgctactc gcgcaagcaa ctcgacgcgg cggcgccatc gtgttatccg
     7321 gcattctccg cggccaggcg tgcgaagtcg aagaggctta tctgcaatgg ttcggcatgc
     7381 gtaagacgga tgaagattcc ggttgggtgt gtctagccgg aaccaagagg tgagcgcgat
     7441 gagcatggtc accacttgcc caagctgcca caccacgttc cgggtgcggc acaaggattt
     7501 gcaggcgggc gagggccgag tgcgttgcgg ccagtgcacg gaagttttca acgccctcga
     7561 cagccttgtc acgctggacg atgcgccggg tgccgtgcgc gaaaccgtcc tgcaagcgcg
     7621 cagcatgccg cagaaaccca gcgccccgga aacgataccc aacctgcaaa ccaaggtgag
     7681 cggcaagcag gcggaggaac cgctcaaatt caccgacgag gctttggaaa aagcggaacg
     7741 cagtccagcc aaaacaatcg cttggtctgc tgctgcgttg ctgctgcttc tcctgttggc
     7801 ggcgcaggtg gcttattttt tccgcgtgcc gattgccgct tactatcccg aaaccaagcc
     7861 ctttttgcag cgctattgcg agctgttgca gtgcgcggtg gatttgccgc gctatgccga
     7921 atggataagc atcgaagcct ccgatttgca ggccgacccg cagcggccga acctcatcat
     7981 tctcaccgcc acgctgcgca atcgcgcgac gttcgtccaa gcctatccgg cattggaact
     8041 cacttttacc aatactcagg cccagatggt ggcgcgccgc gtgtttactc ccgcggacta
     8101 tctggcgagc ggcgtggatg cgagccgcgg catggaagca aacaacgaat tcaccaccaa
     8161 gctttttatc gacagcggcg acctcgagcc ttccggttac cgtttgttcc tgttctatca
     8221 ataagcgagt tgatcataaa aatatgcttg gttgcgcgtg ccttacatgc gaggcatact
     8281 ttacgaaatc tgctttttta tttcaggcaa gtaaattaag gagaacccgt catggcaaaa
     8341 gtcactctcg caggcaaccc catcacagtt gcaggccatt tcccggccgt aggccagatg
     8401 gcgccggatt tcaagctggt taacaaggat ctcaaggatg tgagtctcaa ggatttcgcg
     8461 ggaaagcgca aagtactcaa catcgtgccc agcctcgata ctccgacctg ccagaaatcc
     8521 acgcgccgat tcaatgaaga ggcgaacaag ctcaacaata ccgtggtgct ggtgatttcc
     8581 gccgatttac cgtttgcgca agcgcgcttt tgcggcgctg aaggcttgaa caatgtgatg
     8641 accctttcca ccatgcgcgg cgcggagttc atgaaaaact acggcgtggc gattaccgat
     8701 tcgccgcttg gcggcgtgac tgcgcgcgcg gtggtggtgc tcgacggcga cgacaaggtg
     8761 ctgtatacgg agatggtgcc ggaaatcaag aacgagccga attacgacgc ggcgctgaaa
     8821 gcgctgcgat aacgccgggt ccggcaggac gagatggatt gccggcggtt aaatttaatc
     8881 gccttattcg cgccgggcaa ggcaaagccc ctggcggcgg acatcacgcc cgatgccagc
     8941 gagatattca ttccagtgcc ggacatcaaa gcgggcaggt taagccgact gcagacgcag
     9001 cattagccga agcgccatgg aaaccccgga caacccgcag
//
LOCUS       DATIOW010000112        10618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1680158, whole genome shotgun sequence.
ACCESSION   DATIOW010000112 DATIOW010000000
VERSION     DATIOW010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10618
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680158"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VLL03_03685"
     CDS             <1..247
                     /locus_tag="VLL03_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HSS46499.1"
                     /translation="VFLTASAEVRAERRYKQLMEKGMYARISTLLQEIKERDWRDSQR
                     PVAPLRQGADAGLLDTTSLSIEETVAEVLSRYRQATS"
     gene            334..2052
                     /gene="rpsA"
                     /locus_tag="VLL03_03690"
     CDS             334..2052
                     /gene="rpsA"
                     /locus_tag="VLL03_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HSS46500.1"
                     /translation="MMVTASSGSDSPENFAALFEESLSHQEMRIGEVITAEVMRVDSN
                     YVVVNAGLKSESFISVDEFRNDKGEVEVKPGDFVEVAIEALEDGYGETRLSRDKAKRL
                     TAWRELEVALEQGTLVHGLINGKVKGGLTVMVNGIRAFLPGSLVDIRPVKDTNPYEGK
                     EMEFKVIKLDRKRNNVVVSRRAVLEASQGAERQALLENLKEGAVVKGVVKNITDYGAF
                     VDLGGIDGLLHITDLAWRRVRHPSEMLAVGDEVEAKVLKFDQEKNRVSLGLKQLGDDP
                     WVGLSRRYPQGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVAQL
                     GDEVEVMILEIDEERRRISLGMKQCQPNPWEEFSLNHKKNDHVKGQIKSITDFGIFIG
                     LPGGIDGLVHLSDLSWSQPGEQAVRNYKKGEEVEAVVLSIDVERERISLGIKQLDGDP
                     FTSYIATHEKNSVVAGKVKSTDAKGAVVQLDTDVEGYLRASEMSRDRVEDIRTHLKEG
                     DEVKAMIINIDRKNRSINLSIKAKDLADESEAIQKVAADNTASTGTTNLGALLKAKLD
                     VKNTEQ"
     gene            2062..2346
                     /locus_tag="VLL03_03695"
     CDS             2062..2346
                     /locus_tag="VLL03_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HSS46501.1"
                     /translation="MTKSELIAKLATRYPQLVAKDAELAVKMILDAMTKSLAQGNRIE
                     IRGFGSFGLNHRPPRVGRNPKSGEKVKVPEKYVPHFKAGKELRERVDDKK"
     gene            2431..2703
                     /locus_tag="VLL03_03700"
     CDS             2431..2703
                     /locus_tag="VLL03_03700"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HSS46502.1"
                     /translation="MRYLSWLLKFILFLLLVGFALKNTETAVLRYYLGFEWQAPLVVI
                     LLVFFCAGAAIGVVAALSYIFRQRREILSLKRELRLKHQATDRPVT"
     gene            2716..3891
                     /gene="lapB"
                     /locus_tag="VLL03_03705"
     CDS             2716..3891
                     /gene="lapB"
                     /locus_tag="VLL03_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HSS46503.1"
                     /translation="MEIELWWLLAFPLFFSLGWIAARIDIKQLLSESRALPLSYFKGL
                     NFLLNEQPDKAIEAFIEVVKVDPQTVELHFALGNLFRRRGEVERAIRMHQNLAERADL
                     AQEQKLNAMFELAKDYLKAGLLDRAEELFGKLQGTTHAEAALKNLLEIYQQEKDWQKA
                     IDTARLLQKITNQSHQKEIANFYCELAANELMHSRLENVGPHLDAALATHRKCARASI
                     LQGDLAVLQNRHQQAIAAWKRIETQNPAYMSLVAEKILNAYRHLDRLDEGVRLLRGYL
                     SNYSSLDLLNVVFQATLTLQGAEPAYQLVRDELKRNPTLLGLDKLLEAQLLEAPLERR
                     HDLELIKNLVHSHTRSLALYRCENCGFRARQFYWRCPACGGWETYPPRRTEEHELAA"
     gene            3888..4598
                     /gene="pyrF"
                     /locus_tag="VLL03_03710"
     CDS             3888..4598
                     /gene="pyrF"
                     /locus_tag="VLL03_03710"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008304021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HSS46504.1"
                     /translation="MSEPRVIVALDYPDAKAALHLVDRLEPRACRVKVGEELFTAAGP
                     QLVEKLTGRGFGVFLDLKFHDIPHTVAQACKAAAKLGVWMLNVHALGGKRMLLAAREA
                     LQEFKQRPRLVAVTMLTSMKSEELGEIGVSGKLNEAVLRLAELAHDSDLDGVVCSAQE
                     APDLRKQFGRKFCLVTPGIRPARVTRDDQQRATTPRRAMENGADYLVIGRPITQAQDP
                     LKALQEINQEIAKTGKQK"
     gene            4595..5548
                     /gene="rfaE1"
                     /locus_tag="VLL03_03715"
     CDS             4595..5548
                     /gene="rfaE1"
                     /locus_tag="VLL03_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009908913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HSS46505.1"
                     /translation="MKKTSARKKIAPAALAKARLLVVGDVMLDRYWFGEVSRISPEAP
                     VPVVKIEKVEERPGGAANVARNVAALGAKVELLSVIGNDEASTCLQRLLRDEGIPSQL
                     HRDQAIATTVKLRVIGRQQQLLRIDFETPPGHEVLLSKLADFEKKLSACDVVILSDYA
                     KGGLTHIARMIALANRAGKTVLVDPKGDDYEQYRNATVLTPNRAEFRQVMGAWKNEKE
                     FAAKARQLMRKLSLKALLVTRSEEGMTLFSDGGMLHVPAQAREVYDVSGAGDTVIAAL
                     GVMLASGADLPYAVRIANRAAGIVVGKFGTAVVHRDELFAV"
     gene            5557..6546
                     /gene="rfaD"
                     /locus_tag="VLL03_03720"
     CDS             5557..6546
                     /gene="rfaD"
                     /locus_tag="VLL03_03720"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HSS46506.1"
                     /translation="MYAIVTGAAGFIGSNLVKALNQRGETDIIAVDNLQQSAKFTNLV
                     DCEIADYLDKDVFLERLDEGEFDGAIRVILHQGACTDTSESDGRYMMENNYRYSVELL
                     DYCEDEEVPFIYASSAAIYGVSKVFEEEREHEAPLNVYGYSKFLFDQAVRKRLGQRSA
                     QIAGLRYFNVYGPRERHKGRMASVALHFFDEYRGDGKVKLFEGSSGYAAGEQRRDFIS
                     VEDVIKINLFFLDHPEKSGIFNVGTGASQTFNEVAVAAVNACRKGQPVLTLAQMQQQG
                     LIEYIPFPEKLVGKYQSYTQADIGALRHAGYDQPFLDVQQGVPRYIECLLQQT"
     gene            6626..7513
                     /gene="cysM"
                     /locus_tag="VLL03_03725"
     CDS             6626..7513
                     /gene="cysM"
                     /locus_tag="VLL03_03725"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006476564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HSS46507.1"
                     /translation="MYKTIEHFVGDTPLVKLKRIPGKTTNTILVKLEGDNPAGSVKDR
                     PALWMITRAEQRGQIKPGDTLIEATSGNTGIALAMCAAMMGYRMILVMPEHLSLERRQ
                     TMRSFGAEFVLTPEKGGMEAARDTAERMRDEGMGIILDQFANPDNPLSHYEGTGPEIW
                     RDTGGQITHFVSSMGTTGTIMGCSRYFKQKNPKIEIIGCQPQEGSQIPGIRKWPEAYL
                     PKIFDRKRVDRVIEVSQQDAEEMTRSLASEEGIFAGISSGGALWAALQVSAEVENAVI
                     VSIVCDRGDRYLTTGVFPA"
     gene            7510..8289
                     /locus_tag="VLL03_03730"
     CDS             7510..8289
                     /locus_tag="VLL03_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HSS46508.1"
                     /translation="MTPVLAFDIESVPDIEGLRRLYALRPEVKAGDVAEMAFQMRRQT
                     TGTDFLPLHLHRVVAIACALRDRDGFTVWSLGDVHTGEAELIKRFFDGVEKYTPQLVS
                     WNGGGFDLPVMHYRGLIHGVRAARYWDLGEDDKDFKWNNYISRYHQRHLDLMDLLALY
                     QPRNNVPLDEMAKLIGFPGKLGMEGAGVWEAYQAEKIAEIRNYCETDAVNTYLLYLRF
                     QLMRGVFSEDQYRRECDVVRANLSKSAEPHWREFLNEWRAA"
     gene            8304..9605
                     /gene="rlmD"
                     /locus_tag="VLL03_03735"
     CDS             8304..9605
                     /gene="rlmD"
                     /locus_tag="VLL03_03735"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HSS46509.1"
                     /translation="MSSQAVIESLDQEGRGIAHVGGKTAFIEGGLPGETVVYSPYCKK
                     PNYELGNAEHVLRSSPMRVTPRCPYFGVCGGCSLQHLDVRAQVAAKQRTLEDDLKHIG
                     KVEAETLLPPIYGPTWEYRHRARLSVHYVAKKGGVLVGFHEKKSSFVADMQSCEILPQ
                     RISALLRPLRELIGSLTLRERIPQLELAIGETADVLVLRHLDALSNSDKEALEAFAER
                     HRVQFYLQPAGAESAHPFYPLEPQELYYTLPEFAVRIAFQPTDYTQVNHPMNRMLVRR
                     AMQLLDPRPGERIADLFCGLGNFTLPIARLGAEVLGAEGNAVLLQRAQQNAAQNGLAG
                     KARFQVANLFEPAEASLGCGRFDKILLDPPRDGAIEVVKALAADSPGRIVYVSCSPAT
                     LARDAAVLVRVKGYQLKAAGVVNMFPHTSHIESIALFERAK"
     gene            complement(9648..10337)
                     /locus_tag="VLL03_03740"
     CDS             complement(9648..10337)
                     /locus_tag="VLL03_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HSS46510.1"
                     /translation="MQQDIQISAQTTQLALTQHKVLRNTYMMLGLTMIPTVIGALVGT
                     TSNFSFLAQYPIMGPLLMLAAMFGLMFAVSAARNSIWGVVLLLAFTFVMGWWLGPMLQ
                     YALHFRNGGQLVGMAAGGTGVIFFTLAGIATVTRKDFSFMGKFLLVGLVLLVIASIAN
                     LFFQVPAASLTISAIAVLLFSGYILYDISQIVHGGETNYIMATLKIFLDIYNIFVNLL
                     YLLLAITGQRD"
BASE COUNT         2428 a         2889 c         3181 g         2120 t
ORIGIN      
        1 agtgtttctg accgccagtg ctgaggtgcg cgccgagcgc agatataaac agttgatgga
       61 aaaaggaatg tatgctagga tttcaaccct tttgcaggaa atcaaagaac gcgattggcg
      121 tgacagtcag cgacctgtag cgcccctgcg acagggagcc gatgccggtc ttttggatac
      181 cacctcatta agcatcgagg aaacggtcgc cgaagtgctt tccaggtatc gccaggccac
      241 atcctgatta ggtgtttcgg gttgcccaaa cccgggacaa tgataaacga accccgtggg
      301 aaatcacggg cttaaatccg cgggcgtaaa taaatgatgg ttactgcttc ctcaggttcc
      361 gacagccctg aaaattttgc cgcgctgttt gaagaaagcc tttcccacca ggaaatgcgt
      421 atcggcgagg tgattaccgc cgaagtgatg agagtcgatt ccaattatgt ggtcgtgaac
      481 gccggcctca aatccgaaag ctttatttcc gtagatgaat tccgcaatga caagggcgaa
      541 gtcgaagtca agcccggcga tttcgtggaa gtggcgatcg aagccctcga agacggttac
      601 ggcgaaaccc gcctctcccg cgacaaagcc aagcgcctga cggcatggcg cgagctggaa
      661 gtggcgctgg aacagggcac actggtgcat ggattgatta atggcaaagt caaaggcggg
      721 cttaccgtaa tggtgaacgg catccgcgct ttcctccccg gctcgttggt ggacatccgc
      781 ccggtcaagg acaccaatcc ttacgaaggc aaggaaatgg aattcaaggt catcaagctg
      841 gaccgcaagc gcaacaacgt ggtggtatcg cgccgcgcgg tgctggaagc gagccagggc
      901 gcggaacgcc aggcgctgct ggaaaatcta aaagagggcg cggtggtgaa gggcgtggtg
      961 aagaacatca ctgattacgg cgcgtttgtg gacttgggcg gcatcgacgg tcttctgcat
     1021 atcaccgatc tggcctggcg ccgcgtgcgc cacccttcgg aaatgcttgc cgtaggcgat
     1081 gaagtggaag ccaaggtgct caagttcgac caggagaaaa accgcgtctc attgggcctc
     1141 aagcaactgg gcgacgatcc gtgggtggga ttgtcccgcc gttatccgca aggcacccgc
     1201 ctgttcggca aggtgagcaa cctcaccgat tacggcgcgt ttgtcgaaat cgagcagggc
     1261 atcgaaggcc tcgtgcatgt ctcggagatg gattggacca acaagaacgt gcacccctcc
     1321 aaagtggcgc agctcggcga cgaggtcgag gtgatgattc tggaaattga cgaagagcgc
     1381 cgccgcattt cgctgggcat gaagcagtgc cagcccaatc cctgggaaga attctcgcta
     1441 aaccacaaga agaacgacca cgtcaaaggt cagattaaat ccattaccga tttcggcatt
     1501 ttcatcggcc tccccggcgg cattgacggg ctggtgcatc tttctgatct gtcctggagc
     1561 cagccgggcg aacaggcggt gcgcaattac aagaagggcg aggaagtgga agcggtggtg
     1621 ctttcgattg acgtcgagcg cgagcgcatt tcgctcggca tcaagcagct cgacggcgat
     1681 ccgttcacca gctacatcgc cacccatgag aaaaacagcg tcgtcgccgg caaggtgaag
     1741 tcaactgacg ccaagggggc ggtggtccag ctcgacactg atgtcgaggg ctacctgcgc
     1801 gcttccgaaa tgtcgcgcga ccgggtcgag gacatccgca ctcaccttaa agagggcgac
     1861 gaggtgaaag cgatgattat caacatcgac cgcaagaatc gcagcatcaa cctttcgatt
     1921 aaagccaagg atctggctga cgaatcggaa gccatacaga aagtggcggc tgacaataca
     1981 gccagtaccg gcaccaccaa tctgggagcg cttctgaaag cgaagctgga tgtgaaaaac
     2041 accgagcaat agagggacaa catgaccaag tctgagctga ttgccaaatt ggcgacgcgc
     2101 tatccgcagc tcgtggcaaa agatgcggaa ctcgccgtaa aaatgatttt ggacgcgatg
     2161 accaagagtc tcgcccaggg taaccgtata gaaatcaggg gttttggcag ctttggcttg
     2221 aaccaccgcc cgccgcgcgt cggccgcaac ccgaagtccg gcgagaaggt caaggttccg
     2281 gaaaaatatg ttccgcattt caaggcggga aaagaactgc gcgagcgtgt tgacgacaaa
     2341 aaataaccag tatttttggt tgagagaaaa aggcggcacg ctgttgcaaa atgccgcctt
     2401 ttttgcatgt taagacccgg gatttgagtt atgcgctacc tttcttggct tcttaaattc
     2461 atcctgttcc tgctgctggt gggtttcgcg ctgaaaaaca ccgaaactgc ggtgttgcgt
     2521 tactatctag gtttcgaatg gcaggcgccg ctagtggtga ttctgctggt gtttttctgc
     2581 gccggcgccg cgatcggggt ggtagccgcc ttaagttata ttttccgaca aagacgagaa
     2641 atattgagtc tgaagcgcga gttgcgcctt aaacaccagg ccacggatcg gcctgtcacg
     2701 tgagtcccgg acgccatgga aattgaactc tggtggctgc tggcgtttcc gttgtttttc
     2761 agcctgggct ggatcgccgc ccgcatcgac atcaagcagc tgctgtccga atcgcgcgcg
     2821 ctgcccctgt cctatttcaa ggggctcaat ttcctgctca acgagcaacc cgacaaggcg
     2881 atcgaagcct tcatcgaagt ggtgaaggtc gacccgcaaa cggtggagct gcacttcgcc
     2941 ttgggcaacc tgttccgccg gcgcggcgaa gtggaacgcg caatccgcat gcaccagaac
     3001 ctggcggagc gcgccgatct ggcgcaggaa cagaaactca acgccatgtt tgagctcgcc
     3061 aaggattatc tgaaagcggg gttgctggat cgcgctgagg agctcttcgg caagctgcag
     3121 gggacgacgc atgcggaagc ggcgctgaaa aaccttctgg aaatttatca gcaggaaaag
     3181 gattggcaga aagcgataga caccgcccgg ctactccaaa aaattaccaa ccagtcgcac
     3241 cagaaagaaa tcgccaattt ttactgcgaa ttggcggcca atgagttgat gcattcgcgg
     3301 ctggaaaatg tcggaccgca tctcgatgca gcgctcgcga cccaccgtaa atgcgctcgc
     3361 gccagcatct tgcagggcga tctggcggtg ctgcaaaacc gccaccagca ggcgattgcg
     3421 gcctggaagc ggattgaaac ccagaacccc gcctacatgt cgctggtcgc ggagaaaatt
     3481 ctcaacgctt accggcatct ggaccgcctc gacgaaggcg tgcgtttgct gcgcggctat
     3541 ctttccaact actcttcgct ggatttgttg aacgtagtgt tccaggcgac gctcaccctc
     3601 cagggcgcgg agccggccta tcaactggtg cgggatgagc tgaagcgcaa cccgaccttg
     3661 ctcggcctcg acaagctgct ggaagcgcaa ttgctggaag caccgctgga gcgccgccat
     3721 gatctggaac tgattaaaaa tctggtgcac agccatacgc gcagccttgc gctttaccgt
     3781 tgtgaaaatt gcggctttcg cgccaggcag ttttactggc gctgcccggc ctgcggcggc
     3841 tgggaaactt atccgccgcg ccgtaccgaa gagcacgaac tggcggcatg agcgaaccgc
     3901 gggtcatcgt tgcattggat taccccgacg ccaaagcggc gctgcatttg gtcgatcgcc
     3961 ttgagccgag ggcttgccgg gtcaaggtgg gcgaggaatt gtttacggcg gcgggcccac
     4021 aactggtgga aaaactgaca ggacgcggtt tcggggtgtt tcttgacctc aagtttcacg
     4081 acattccgca caccgtggcg caggcctgca aggcggcggc gaaacttgga gtatggatgc
     4141 ttaacgtgca tgcattgggc gggaagagaa tgctgctcgc cgcgcgcgaa gcactgcagg
     4201 aattcaagca acggccgaga ctggtcgcag ttaccatgct taccagcatg aaaagcgagg
     4261 agcttgggga aatcggcgtc agcggtaaac tgaacgaagc ggtgctgcgg ctggcggagc
     4321 ttgcccatga cagcgatctg gacggcgtgg tgtgctcggc gcaggaagcg ccggatttgc
     4381 gcaagcaatt cggccgtaag ttttgtctgg taacccccgg cattcgccct gcgagggtta
     4441 ctcgggatga tcagcagcgg gcgacgaccc cgcggcgggc aatggagaat ggcgccgatt
     4501 atctggtcat cggccgcccg attacccagg cacaggaccc gctcaaggca ttgcaggaaa
     4561 ttaaccagga aattgccaag actggaaaac aaaaatgaaa aaaacaagcg cccgtaaaaa
     4621 aattgcccca gccgcgctgg ccaaagcacg cctgctggtg gtgggtgatg tcatgctgga
     4681 ccgttattgg ttcggcgaag taagccgcat ctctcccgaa gcgccggtgc cggtggtgaa
     4741 gattgagaag gtggaggagc gcccgggcgg cgccgccaat gtggcgcgca acgtcgccgc
     4801 gctcggagcg aaagtagaat tgctttccgt aatcggcaat gatgaagcca gcacctgcct
     4861 gcaacggctg ctgcgcgacg aaggcatccc gtcgcaactg caccgggacc aggcgattgc
     4921 gaccaccgtc aagctgcgcg tgattggccg ccagcagcaa ctgctgcgaa tcgatttcga
     4981 gaccccgccc ggccacgagg tgctgctgtc caagcttgcc gactttgaaa aaaaactgtc
     5041 tgcatgtgat gtggtgatcc tgtccgatta cgcgaaaggg ggactcacgc acatcgcgcg
     5101 catgattgcg cttgccaacc gtgccgggaa gaccgtgctg gtcgacccca agggcgacga
     5161 ttacgaacaa tatcgcaacg ccaccgtgct tactcccaac cgcgccgaat tccggcaggt
     5221 gatgggggcc tggaaaaacg agaaagagtt cgccgccaag gcccggcaat tgatgcgaaa
     5281 actcagtctc aaggcgctgc tggtgacccg aagcgaggaa ggcatgacgc tgttcagtga
     5341 cggcggcatg ctgcatgtgc cggcgcaggc gcgcgaagtc tatgacgtaa gcggcgccgg
     5401 ggatacggtg attgccgcgc tgggcgtgat gctggcgagc ggagcggatt tgccgtacgc
     5461 agtgcgcatc gccaaccggg cggcgggcat cgtggtcggc aagttcggca cggcggtggt
     5521 gcatcgcgac gagttgttcg cggtttagga aaaaatatgt acgccatcgt caccggagcg
     5581 gctggattca tcggttccaa cctggtcaag gcgttaaatc agcgtggcga gaccgatatt
     5641 atcgcggtgg acaacttgca acaatcggcc aaattcacca acctagtgga ctgtgaaatc
     5701 gccgactatc tcgataaaga cgtttttctc gagcgcttgg atgaggggga atttgatggc
     5761 gcgatcagag tgattctcca ccagggcgcc tgcaccgaca cctccgagag cgatggccgc
     5821 tatatgatgg aaaacaatta tcgttattcc gtcgaactgc tcgactattg cgaggatgaa
     5881 gaagtgccgt tcatctatgc ttcctcggcc gcaatctatg gcgtaagcaa ggtgttcgag
     5941 gaagagcggg agcatgaagc accactcaat gtttacggct actccaagtt cctgttcgac
     6001 caggcggtgc gcaagcggct cggtcagcgc agcgcgcaga ttgcgggctt acgctatttc
     6061 aacgtttacg gcccgcgcga gcgccacaag ggcagaatgg cttcggtggc gctgcatttc
     6121 ttcgatgaat accgcgggga cggaaaagta aaactgttcg aaggcagcag cggctacgcg
     6181 gccggcgaac agcgacgcga ttttatttcg gtcgaagacg tgatcaagat caacctgttc
     6241 ttcctcgatc atccggaaaa atccggcatc ttcaatgtgg gcactggcgc ctcacagacg
     6301 ttcaacgaag tcgcagttgc cgcagtgaat gcctgccgca aaggccagcc ggtgttgacc
     6361 cttgcacaaa tgcagcagca gggcttgatt gaatacattc cttttcccga aaagctcgta
     6421 gggaaatacc agagctatac ccaggcggat atcggcgccc tgcgccatgc gggctatgac
     6481 cagccttttc ttgacgtgca gcaaggtgtc ccccgctata tcgagtgtct gttacaacag
     6541 acttagcttt ttcaaacccc gctttggcaa ctttagcaag gcatcgggta ttatattaat
     6601 cggcgccttt tgaaagaatt gctgcatgta taagaccata gaacatttcg tcggtgatac
     6661 cccgctggtt aagttaaaac gcatcccagg aaagaccacc aacactatcc tggtgaagct
     6721 cgagggcgac aatccggcgg ggtcggtgaa agaccgcccg gcgctgtgga tgattacgcg
     6781 cgccgaacaa cggggacaaa tcaagcccgg cgacacgctg atcgaggcga ccagcggcaa
     6841 taccggtatc gcactggcga tgtgcgcggc gatgatgggc taccgcatga ttctggtgat
     6901 gccggagcat ttaagcctgg agcggcggca gaccatgcgc tcgttcggcg cggaattcgt
     6961 attgacgccg gaaaagggcg gcatggaagc ggcgcgcgat accgccgagc gcatgcgcga
     7021 tgaaggcatg ggtatcattc tcgaccagtt tgccaatccc gataacccgt tgtcgcacta
     7081 cgaaggcacc ggaccggaaa tctggcgcga caccggaggt caaattaccc atttcgtgag
     7141 cagcatgggc acgaccggca ccatcatggg ctgctcgcgc tacttcaagc agaagaaccc
     7201 gaagatcgag atcatcggct gccagcccca ggagggctcg cagatcccgg gcatccgcaa
     7261 atggcccgag gcctatcttc caaaaatctt tgaccgcaag cgggtggatc gcgtcattga
     7321 ggtgagccag caggacgcgg aggaaatgac ccgcagccta gcgagcgagg aaggcatttt
     7381 cgccggcatt tcctcgggcg gcgcgctgtg ggcggcgctg caggtttccg ctgaagtcga
     7441 aaacgcggtc attgtctcca tcgtttgtga ccgcggcgac cgttatctaa ccaccggagt
     7501 tttcccggcg tgacgcctgt tcttgcgttc gacatcgagt cggttcccga tatcgaaggt
     7561 ctgcgccggc tctacgcgct gcggcccgag gtcaaagccg gcgatgtcgc ggaaatggcg
     7621 tttcagatgc gccgtcagac gactggcacg gattttcttc ccttgcatct gcatcgcgtc
     7681 gttgctattg cttgcgcgct gcgcgaccgc gacggtttca ccgtttggtc gctgggggat
     7741 gttcacaccg gcgaggcgga gctcatcaag cgtttttttg acggcgtcga aaaatacacc
     7801 ccccaactgg tttcctggaa cggcggcggc tttgatctgc cggtcatgca ttaccgcgga
     7861 ctaattcacg gcgtgcgcgc cgcccgctac tgggacctag gcgaggacga caaagatttc
     7921 aagtggaaca attacatcag ccgctatcat cagcgccatc tggacttgat ggatttactc
     7981 gcgctttacc agccgcgcaa caacgtgccg ctcgatgaaa tggcgaagct catcggtttt
     8041 cccggcaagc ttggcatgga aggcgccggg gtgtgggaag cttaccaggc cgagaaaatc
     8101 gcggaaattc gcaattattg cgaaaccgat gccgttaaca cctatctgct ttatctgcgc
     8161 ttccaattaa tgcgcggcgt gttcagcgaa gaccaatacc gccgcgaatg cgatgtggtg
     8221 cgcgcgaatt taagcaaatc cgccgagccg cactggcgtg aattcctgaa cgagtggcgc
     8281 gcggcctgat aacggatttg tccttgtcct cccaagccgt aattgaatcc ctggaccagg
     8341 aaggccgcgg tattgcccat gtcgggggaa aaacggcttt catcgagggc ggtcttccgg
     8401 gcgaaaccgt cgtttattct ccgtactgta aaaaacccaa ctacgagctg ggtaatgcag
     8461 agcatgttct gcgctcgagc ccgatgcggg tgacaccgcg ctgcccgtat tttggcgtgt
     8521 gcggcggttg cagcctgcag catcttgatg tgcgcgccca ggttgcggcg aaacaacgca
     8581 cactggaaga tgatctcaag catatcggca aggtcgaagc ggaaacgctg ttgccgccca
     8641 tatacggccc gacttgggaa taccgccacc gcgcgcggct gtcggtgcat tatgtggcaa
     8701 aaaaaggcgg agtattggtg gggtttcacg aaaagaaaag cagctttgtc gccgacatgc
     8761 aaagctgcga aattttgccg cagaggattt cagcgctgct taggccgtta cgcgaattaa
     8821 tcggcagcct gacgctgcgc gagcgcattc cgcagcttga gttggcaata ggggaaactg
     8881 ccgatgtact ggtgttgcgg catcttgacg ccttgagcaa cagtgacaag gaagcgctcg
     8941 aggccttcgc cgaacgtcat cgtgtgcagt tttatctgca acctgcggga gcggaatccg
     9001 ctcatccttt ttatccgctg gagccgcaag agctttatta cacgcttccc gagtttgccg
     9061 ttcgcatcgc attccagcct accgattaca cccaggtcaa ccacccgatg aaccgcatgt
     9121 tggtgcgccg cgccatgcaa ctgcttgacc ctaggccggg cgaacggatt gccgatttgt
     9181 tttgcggcct ggggaatttt accttgccca tagcgcggct gggcgcggag gtgttgggcg
     9241 cggaagggaa tgcggtgttg ttgcaacgcg cacaacagaa cgcggcacaa aacggattag
     9301 ctggaaaggc gcgcttccag gttgccaacc tgtttgagcc ggccgaggcc tcattgggct
     9361 gcggccggtt tgacaagata ttgctcgatc cgccgcgcga cggggccatt gaagtagtca
     9421 aagccttagc tgccgattcg ccgggccgca tcgtttatgt ttcctgcagt ccggcgactt
     9481 tggcgcgtga tgcggcggtg ctggtgcgtg tcaaaggcta tcaactcaag gcagcggggg
     9541 tcgtcaacat gtttccgcac acttcgcaca ttgaatccat cgcgctgttc gagcgggcaa
     9601 aataaaaagg cgggcgcagg cccgcctttt acatgagttg cttgcgttta gtctcgctga
     9661 ccggtaatag ccaacagcag gtacagcagg ttgacgaaga tattatagat gtcgagaaag
     9721 attttcagcg tggccatgat gtagttggtt tcgccgccgt ggacgatttg gcttatgtca
     9781 tacaggatgt agccggagaa cagcaacacc gcaatcgccg aaatcgtcag gcttgccgcc
     9841 ggtacctgga agaacaggtt agcgatggaa gcaatgacca gaagaaccaa accgaccagc
     9901 aggaacttgc ccatgaagct gaaatccttg cgggtcacgg tggcgattcc cgccagcgtg
     9961 aagaaaatca cgccggttcc cccggcagcc attcccacca attgcccgcc gtttctgaag
    10021 tgcagcgcgt attgcagcat cggccccagc caccagccca tcacgaaagt gaaagccagc
    10081 agcaatacca caccccagat gctgttacgg gcggcgctga ctgcgaacat caagccgaac
    10141 attgccgcca gcatcaacag cggccccatg atcggatatt gcgcgagaaa ggaaaaattg
    10201 ctcgtggttc caaccagtgc gccgatgacc gtcggaatca tggtcaatcc cagcatcatg
    10261 taggtgttgc gcaacacctt atgctgggtc agcgccagtt gcgtcgtttg tgcgcttatc
    10321 tgaatgtctt gttgcattgc agccgctcct taaatacttg agcacatgct cagtgcctaa
    10381 gactactcga ctgaggtaaa agttgcaagc cggtaaaaaa cgatacctag ccaaaacaag
    10441 gcgttaagag ttatgatgcc gccctctgga gagttggcag agcggtcgaa tgcggcggtt
    10501 ctggagcacg cgcagcttgc tccagtgaag gctaacctgc gcaaccatgc cggaactgaa
    10561 accgcggggt aaaataatca gcggaaggtt ggccgagcgg ttgaaggcac cggtcttg
//
LOCUS       DATIOW010000113         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1680457, whole genome shotgun sequence.
ACCESSION   DATIOW010000113 DATIOW010000000
VERSION     DATIOW010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680457"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /gene="secG"
                     /locus_tag="VLL03_03745"
     CDS             complement(<1..334)
                     /gene="secG"
                     /locus_tag="VLL03_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HSS46511.1"
                     /translation="METLIWVVHILTALALIGLVLLQHGKGADAGAAFGSGASGSLFG
                     ASGSANFLSRTTAVLAAVFFLTSLGLTYFSSHKSENKGVMELRKEVPAVPADKAPAGQ
                     APAAPAAEG"
     gene            complement(354..1106)
                     /gene="tpiA"
                     /locus_tag="VLL03_03750"
     CDS             complement(354..1106)
                     /gene="tpiA"
                     /locus_tag="VLL03_03750"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HSS46512.1"
                     /translation="MRQKLVAGNWKMHGSLVQNKVLLDALVTGLKSVRKVSCAVCVPY
                     PYLFQARIILRDSNIAWGAQNLSPYDSGAYTGEVSGPMLKDFGCRYVIVGHSERRTLH
                     GEDSRLVAQKFKAAQSAGLIPILCIGETLAEREAGATERVVAGQLEAVLELAGIAAMT
                     NAVVAYEPVWAIGTGKNATPEQAQTVHAFIRQGIAARNPEVARQLPILYGGSVKASNG
                     EQLFAMPDIDGGLIGGASLITDEFLAICAAAQ"
     gene            complement(1579..>2302)
                     /gene="glmM"
                     /locus_tag="VLL03_03755"
     CDS             complement(1579..>2302)
                     /gene="glmM"
                     /locus_tag="VLL03_03755"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006475.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HSS46513.1"
                     /translation="PDGLNINQECGATYPKTLQEAVRQQRADLGIALDGDGDRLLMVD
                     GTGKAYDGDQLLYIIAKHRKQQGTLKGGVVGTLMTNLGMQRALEKIKVPFARAAVGDR
                     YVLELMQIRGWQLGGENSGHIICLDKHTTGDGIVSALQVLHAMRVYREDMAHLTREVA
                     LYPQVLVNVKVAKGFDFSNQRPVRNALSEAEADLGDKGRVLLRASGTEPVVRVMVEGK
                     PQAKVKQWAATIAQAVREAAAK"
BASE COUNT          468 a          743 c          617 g          474 t
ORIGIN      
        1 cgccttctgc cgctggcgcc gcaggcgcct gacccgcagg cgctttatcc gcaggcaccg
       61 cgggaacttc cttgcgcaac tccatcacgc ccttgttttc gctcttatgg ctggaaaagt
      121 aagtcaaccc caggctggtc agaaaaaaca ccgccgccag caccgcagtg gtgcggctca
      181 ggaaattagc cgagccgctt gcgccgaaaa gactccctga ggcaccgctg ccaaaggccg
      241 cgccggcatc cgcccctttg ccatgctgca gcagcaccag gccgatgagg gcaagggcgg
      301 tcaggatatg cacaacccaa atcaaggttt ccaaaaaacg atctccaagt taactactgc
      361 gctgcggcgc atattgccag aaattcatcc gtgatcaggg acgcgccgcc tatcaggccg
      421 ccgtcgatat ccggcatggc gaaaagctgc tcaccattac tggcctttac gctgccgccg
      481 tataaaatcg gcaattgccg cgcgacttcc ggattccgcg cagcgatccc ctggcgaata
      541 aaagcatgca ccgtctgcgc ctgctcgggg gtcgcattct tcccggtgcc aatcgcccat
      601 accggttcat acgccaccac ggcgttggtc atcgctgcga ttccggcaag ctccagtacc
      661 gcttcgagct gccccgccac gactcgttca gtggcccccg cttcgcgctc cgccagggtt
      721 tcgccaatgc atagaatcgg tatcaagccc gcgctctgcg ccgccttgaa cttctgcgcc
      781 accaagcggc tgtcttcgcc atgcagcgtg cggcgctccg aatgcccgac gattacatag
      841 cggcagccga agtccttgag catcggcccc gacacttccc cggtgtaagc acctgaatca
      901 taggggctca aattctgcgc gccccaggca atgttgctgt cgcgcaggat aatccgcgcc
      961 tgaaacagat aagggtaggg cacgcacacc gcgcacgaca ccttgcgcac cgatttcaga
     1021 ccggttacca gagcatcgag caataccttg ttttgcacaa ggctgccatg cattttccag
     1081 ttgccggcaa ccagcttttg gcgcatccat gtccttagga atctctgatt aagtccatcg
     1141 cgtacgcgac agcgtgcaat ccgggtgcag ggcgaggcgt gaggagcgcc gtttggtgga
     1201 ggccaaataa gcgacgaaca acgaagccct gtgcacgaat tgccctgtcc cgccagggtt
     1261 gcggcgaccg cgcggccatc tgcggtgttg ctcgggcttg cgttatggct tgatagcgct
     1321 gcgccctcgc gcctagcatc tggccgccct tcgcccgcaa cgcgttacgc gcgggactta
     1381 atcagaggtt ccatacgtca aatcttttga attttacctt tgaacagccc ccagggtcaa
     1441 tttcagcttg caacagatgc gctgtaatga agcgaattta gcgccgggaa aacaagatag
     1501 ggagcagctt taatggaaag ggccacaaat ttttgcggat gatcgcggcg cgcaaaattc
     1561 acgcgcggaa aaaataattt attttgcggc tgcctcgcgc accgcttggg caatagtggc
     1621 ggcccattgc ttgactttgg cctggggctt gccttccacc atcacccgca ccactggctc
     1681 ggtgcccgaa gcgcgcaaca gcacccggcc tttgtcgccg agatccgctt ccgcctcgct
     1741 gagcgcattc cttaccggcc gctggttgct gaaatcaaat cctttggcga ctttgacatt
     1801 caccagcact tgcggataga gcgcaacctc gcgggtcaaa tgtgccatgt cctcccgata
     1861 aacacgcatc gcgtgcaaaa cctgcaacgc ggagacgatg ccatcgccgg tagtgtgctt
     1921 gtccaggcag atgatatgtc ctgaattctc tccgcccagc tgccagccgc ggatttgcat
     1981 gagctccagc acataacggt cgccaactgc tgcgcgggca aatggcacct tgattttttc
     2041 cagggcgcgc tgcatgccga gattggtcat cagcgttccc accacaccgc ctttgagcgt
     2101 gccctgctgc ttgcggtgct tagcgatgat atagagcaat tgatcgccat cgtaagcttt
     2161 ccccgtgccg tcaaccatca gcagacggtc gccgtcacca tccagcgcaa tccccagatc
     2221 ggcacgctgc tgcctcaccg cttcctgcag cgttttgggg taagtagcgc cgcattcctg
     2281 attgatgttc aagccatcgg gc
//
LOCUS       DATIOW010000114         1877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_664764, whole genome shotgun sequence.
ACCESSION   DATIOW010000114 DATIOW010000000
VERSION     DATIOW010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664764"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..501)
                     /locus_tag="VLL03_03760"
     CDS             complement(133..501)
                     /locus_tag="VLL03_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004528999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HSS46514.1"
                     /translation="MTGLADFILVVHFCFVLFVVGGLFLIWLGAARGWRWVRNFWFRS
                     AHLAAMLFVAGESLAGWICPLTVWEDTLRGSAGGSSFIQRWLGKLLYYDFPEWVFITV
                     YLLFALAVVITFIRVRPERP"
     gene            complement(509..1348)
                     /locus_tag="VLL03_03765"
     CDS             complement(509..1348)
                     /locus_tag="VLL03_03765"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HSS46515.1"
                     /translation="MAHTTLVSTSLLAKHLDDPRWVICDCRHDLADPGAGRRAYIAAH
                     IPSARFVNLDADLSAPPTGSNGRHPLPAPEKFCETLGRLGIDRKKQVVAYDSAGGAFA
                     ARLWWMLRWQGLDSVAVLDGGFPKWLAENRPASSEMPRIEPVTFAPKPQNNSVDVRFV
                     ETHLGKGDMLLIDARSPERYNGSIEPLDPAAGHIPGALNHFFQNNLDKGCFKPPSALR
                     QVFSGLLDKTPPQNVVHTCGSGVTACQNVLAMEIAGLSGSKLYPGSWSEWCADLRRPM
                     ITK"
     gene            complement(1354..1698)
                     /locus_tag="VLL03_03770"
     CDS             complement(1354..1698)
                     /locus_tag="VLL03_03770"
                     /inference="COORDINATES: protein motif:HMM:NF023390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HSS46516.1"
                     /translation="MISIQHAGNLVTANILGEFTISDYREFEERVLEKLRSLKKINLS
                     IDLRDMVGYTLDVAWEDIRFSRSHEHDFGKIAIITDNQWLTWSAWLSRLFVDADIRVF
                     EDDRSAQDWLKA"
BASE COUNT          418 a          545 c          549 g          365 t
ORIGIN      
        1 gcgagcgcac gggtgaccca cagttgctgc agtatttcat gttcgttcct gattgcgctt
       61 attataccgc aagctgccgg gcttcccgca aagacagaga aaatttgcag gaatgggaat
      121 tgaaaaaaat cttcatgggc gctcgggcct gacgcggatg aaagtaatca ccacagccag
      181 ggcgaatagc aggtaaaccg tgatgaaaac ccattcggga aaatcgtagt aaaggagttt
      241 gcctagccag cgttggatga aactggaacc gccggcgctg ccgcgcaaag tgtcttccca
      301 cacggtcaag gggcagatcc aaccggcaag cgactctccc gccacaaaca gcattgcggc
      361 caggtgcgcg ctgcgaaacc agaagttgcg cacccagcgc cagccccgcg ccgcgcccag
      421 ccatattaga aacagcccgc cgaccacgaa cagcacaaaa cagaaatgaa ctacaagaat
      481 aaagtcggcg agcccggtca cgggtttgtt atttggtaat catcggccgc cgcaggtcgg
      541 cgcaccactc gctccacgag cccgggtaaa gcttcgagcc ggaaagtccc gcgatttcca
      601 tcgccagcac attctggcac gcggtcacgc ccgagccgca cgtatgcact acgttttgcg
      661 gcggagtttt gtcaagcaat cctgaaaata cctgccgcaa tgctgagggg ggtttgaagc
      721 agcctttatc gaggttgttc tggaaaaaat gattgagtgc gccgggaatg tgtcccgccg
      781 ccgggtcgag cggctcaata ctgccgttgt agcgctcggg gctgcgcgcg tcgatgagca
      841 gcatgtcacc cttccccagg tgcgtttcga caaagcgtac atcaaccgaa ttgttttgcg
      901 gcttgggagc gaatgtgacc ggctcaatac gcggcatttc actgcttgcg gggcgatttt
      961 cggcgagcca cttggggaat ccgccgtcca gcacggcgac gctgtcaagt ccttgccagc
     1021 gcagcatcca ccacagccgc gctgcgaacg cgccgcccgc gctgtcgtag gccacgactt
     1081 gttttttgcg gtcaatgccg agcctgccga gcgtctcgca gaatttttcc ggcgcaggca
     1141 ggggatgcct gccattgctt ccagtgggcg gcgccgagag gtcggcatcc agattcacga
     1201 accgcgccga gggaatgtgc gcggcaatgt aagcgcggcg ccctgcgccg gggtcggcaa
     1261 ggtcgtggcg gcaatcgcag atcacccagc gcggatcatc gagatgcttt gcgagcaagc
     1321 tggtcgagac gagagtggta tgggccatgg tgttcaagct ttaagccagt cctgcgccga
     1381 ccggtcgtcc tcaaacactc gaatatcggc atccacgaac aggcgtgaga gccaggcgct
     1441 ccaagtcagc cactgattgt cggtaatgat cgcgattttt ccgaaatcat gctcgtggct
     1501 gcggctgaag cggatatctt cccacgccac gtcgagtgtg tagcccacca tgtcgcgcag
     1561 atcaatcgac aagttgattt ttttgagtga acgcagtttt tccaacacgc gctcttcgaa
     1621 ttcccgatag tcggaaatgg taaattcgcc cagaatattg gcggtgacca gattgcctgc
     1681 atgttgaatt gagatcatcg agggctcctt tttaatatta ggcaactacg gccgcaacag
     1741 caacgtcact catcgggcat gccccgaggc ggagcggctt tgatgctgag aatgccgctg
     1801 gccgcgatga gggcgatgcc cagccagcca gccacgggca gcacttcccc ccacagcgcg
     1861 atgccgaata gcgaagc
//
LOCUS       DATIOW010000115         4388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_970246, whole genome shotgun sequence.
ACCESSION   DATIOW010000115 DATIOW010000000
VERSION     DATIOW010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970246"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="VLL03_03775"
     CDS             complement(<1..172)
                     /locus_tag="VLL03_03775"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796187.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSS46517.1"
                     /translation="MATYQELSDFLAEVERRAFKQALCAVRDEEAALDIVQDSMLKLA
                     EKYAGKPPAELPM"
     gene            310..2016
                     /locus_tag="VLL03_03780"
     CDS             310..2016
                     /locus_tag="VLL03_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HSS46518.1"
                     /translation="MELTGAEIVVRCLQEEKIEHIFGYPGGAVLFIYDELFKQDKVKH
                     ILVRHEQGAVHAADGYSRSSNKVGVCLVTSGPGVTNAVTGIATAYMDSIPMIIITGQV
                     PTHAIGLDAFQECDTVGITRPCVKHNFLVKNVKDLAVTIKKAFYLATTGRPGPVLVDI
                     PKDVTQHKCEFEYPETVSMRSYNPVTKGHSGQIKKAVQLLMETKRPMIYTGGGVILND
                     AAKELTRLVRLLGFPCTNTLMGLGGYPATDPLFVGMLGMHGTYEANMAMQHCDVLLAI
                     GARFDDRVIGNPRHFYDPNRRIVHIDIDPSSISKRVKVDVPIVGNVKDVLEEMIKLLE
                     TSKERPDAAALKAWWAQIQQWKSRDCLKYDRASAIIKPQLVLEKLYEVTKGEAFVTSD
                     VGQHQMWAAQFYKFDKPKRWINSGGLGTMGFGLPAAMGAQLANTGATVACVTGEASIQ
                     MCIQELSTCKQYRLPIKVVNVNNRYMGMVRQWQQFFHGNRYSESYMDALPDFVKLAES
                     YGHIGMKIEKPSDVEPALKEAFMRKDDLVFMDFITDQSENVYPMVAAGKGISEMILAE
                     DL"
     gene            2166..2657
                     /gene="ilvN"
                     /locus_tag="VLL03_03785"
     CDS             2166..2657
                     /gene="ilvN"
                     /locus_tag="VLL03_03785"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HSS46519.1"
                     /translation="MRHILSLLLENEAGALSRVAGLFSARGYNIESLTVAPTEDSTLS
                     RMTIVTTGSDDVIEQITKQLNKLVEVVKVVDLNDGEHIERELMLVKVRAAGKDREEMK
                     RMADIFRGRIIDVTDKSYTIELTGPGAKLDAFLQALEHGAILETVRTGASGVGRGERI
                     LKV"
     gene            2672..3688
                     /gene="ilvC"
                     /locus_tag="VLL03_03790"
     CDS             2672..3688
                     /gene="ilvC"
                     /locus_tag="VLL03_03790"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HSS46520.1"
                     /translation="MKVYYDKDANLSLIKGKKITIVGYGSQGHAHANNLQDSGVKVTV
                     GLRKGGASWDKAKKAGLQVKAVGEAVKGADVVMILLPDENHAQVYRDDIEPNIKKGAA
                     LAFAHGFNIHFGQIEPRADLDVIMIAPKGPGHLVRSTYIQGGGVPSLIAVKQDASKKA
                     RDIALSYACAIGATRAGVIETTFREECETDLFGEQVVLCGGLTALIQAGFETLVEAGY
                     APEMAYFECLHEVKLIVDLIYEGGIANMRYSISNTAEYGDFTRGPRIINDKTRAEMKK
                     ILKDIQSGKFAKEFILENRAGTPTLKAMRRIAAQHPIEQVGGKLRGMMPWIKKNKLVD
                     QSRN"
     gene            3775..>4388
                     /locus_tag="VLL03_03795"
     CDS             3775..>4388
                     /locus_tag="VLL03_03795"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HSS46521.1"
                     /translation="MNYPHPLIAREGWPFLAASVIVSVSVVLFAGWLWSAPFWLASLF
                     ILQFFRDPPRNVPGDSYTVVSPADGRIVAVERCLDPYLQRDALKVSVFMNVFDAHSNR
                     SPVDGVVKKDWHHSGKFINAALSKASVDNERKALWLKTVNGFDVTCVQVAGLIARRIL
                     CYVKSGDALVRGQRYGFIRFGSRVDVYLPLNAAVRTGIGDKAYAA"
BASE COUNT          960 a         1221 c         1298 g          909 t
ORIGIN      
        1 gcatcggcaa ttccgccggt ggcttgccag cgtatttttc cgcgagcttc agcatcgagt
       61 cctggacaat gtcgagcgcg gcttcttcgt cgcgcaccgc gcacagcgcc tgcttgaaag
      121 cgcggcgttc cacttctgcc agaaaatcgg agagttcctg ataggtagcc agttaattca
      181 accttgcgag aagaggtgag cgcagcctgc aatgtgccga ttgcgccgtc gcgggcattt
      241 tgcacttgac caaaaatcgg ctacacggta ctgtttaaga ttcttcaagt tatttagcgg
      301 tttaaggcga tggaactgac gggcgcagag attgtagtgc gttgcctgca ggaagaaaag
      361 atcgagcata ttttcggcta ccccggcggc gcggtgctgt ttatatacga cgaactcttc
      421 aagcaggaca aggtcaagca tatcctggtg cggcatgagc agggcgcggt gcatgccgcc
      481 gacggctatt cacgttcctc caacaaagtc ggagtgtgtc tcgtcacgtc cggtcccggc
      541 gtgaccaatg cggtgaccgg catcgccacc gcctatatgg attcgatccc catgatcatc
      601 atcaccggcc aggtgccgac tcacgccatc gggctcgacg cattccagga atgtgacact
      661 gtgggcatca cccgtccctg cgtcaagcac aatttcctgg tgaagaacgt gaaagacctg
      721 gccgttacca tcaaaaaggc attttacctc gccaccaccg gccgtcctgg cccggtgctg
      781 gtggatatcc cgaaagatgt gacgcagcac aaatgtgagt ttgagtaccc ggaaaccgtc
      841 agcatgcgct cctacaaccc ggtgaccaag gggcattccg ggcagatcaa gaaagcggtg
      901 cagctgctga tggaaaccaa gcgcccgatg atttacaccg gcggcggggt gattctcaac
      961 gatgccgcca aggaattgac gcgcttggtg cgcctgctcg ggttcccctg taccaataca
     1021 ctgatgggcc tgggcggtta tccggcgacc gacccgctgt ttgtcggcat gctgggcatg
     1081 cacggcacct atgaggccaa catggcgatg cagcattgcg acgtgctgct cgcgatcggc
     1141 gcgcgcttcg acgaccgcgt gatcggcaat cccaggcatt tctacgaccc caaccgccga
     1201 attgtccaca tcgacatcga tccctcctcg atttccaagc gcgtgaaagt ggacgtgccg
     1261 atcgtcggca acgtcaaaga tgtgctcgag gaaatgatca agctgctcga gacttcgaaa
     1321 gagcggccgg atgccgcggc gctcaaggca tggtgggcgc agatccagca gtggaaatcc
     1381 agggactgcc tgaaatacga ccgcgccagc gccatcatca agccgcagtt ggtgctggaa
     1441 aaactttatg aagttaccaa gggagaggcg tttgtgactt cggacgtcgg ccagcatcag
     1501 atgtgggccg cgcagtttta caagttcgat aagcctaagc gttggatcaa ttccggcggg
     1561 ctgggcacga tgggtttcgg cctgcccgcg gcgatgggcg cgcagcttgc caataccgga
     1621 gcgacggtgg cgtgcgtcac cggagaagcc agcatccaga tgtgcatcca ggagctttcc
     1681 acctgcaaac agtacaggct cccgatcaag gtcgtgaacg tcaacaaccg ctacatgggc
     1741 atggtgcggc aatggcagca gtttttccac ggcaaccgct actcggaatc ctacatggat
     1801 gcgcttcctg attttgtaaa gctcgccgag agctacggcc acatcggcat gaaaatcgag
     1861 aagccttctg atgtcgagcc ggctctcaag gaagcgttca tgcgcaagga tgacctggtg
     1921 ttcatggatt tcatcaccga ccagtcggaa aatgtctacc cgatggtggc tgccggcaag
     1981 ggcatcagcg agatgatcct ggcggaagac ctttgatgaa cagtgtcttt tgggccaata
     2041 ctgcgttgct cggcttttcg cgatgctcac atacttgtcg gctccgctcg ctcaaagcct
     2101 cccgccttgt tttgacccaa agtacttgtt catcagctcc aagttgaaaa acctatatta
     2161 aacacatgcg gcatattctt tctctgcttt tggaaaacga ggcgggcgcg ctgtcccgcg
     2221 tcgccgggct gttttccgcg cgcggctaca acatcgagtc actcaccgtg gcgcccaccg
     2281 aggattccac tctttcgcgc atgaccatcg tcaccaccgg ttcagatgat gtgattgagc
     2341 agattaccaa gcaactgaac aagctggtgg aagtggtgaa ggtggtggat ctgaacgatg
     2401 gcgaacatat cgagcgcgaa ttgatgctgg tgaaggtgcg cgcggcgggc aaagaccgcg
     2461 aggagatgaa gcgcatggcc gacatcttcc gcggccgcat catcgatgtc accgacaagt
     2521 cctacaccat cgaactcacc gggccggggg ctaagcttga cgcttttctc caggccctgg
     2581 agcacggcgc gatactggag acggtgcgca ccggcgcttc cggcgtcggc cgcggcgaac
     2641 gcatacttaa agtctagctc aagaggaaaa catgaaagtc tattacgaca aggatgccaa
     2701 tttatccctc atcaagggaa aaaaaatcac catcgtcggc tacggatcgc aaggtcacgc
     2761 gcacgccaac aacctgcagg attcgggcgt gaaagtgacg gtgggtttgc gcaagggcgg
     2821 cgcgtcctgg gataaggcga aaaaagccgg actgcaagtc aaggcggtcg gcgaggcggt
     2881 gaagggcgcg gatgtggtga tgattctgct tcctgacgag aaccacgcgc aagtttaccg
     2941 cgacgacatc gagcccaaca tcaaaaaggg tgcagcgctg gcgttcgcgc acggtttcaa
     3001 catccacttc ggtcagatcg agccgcgcgc ggacctcgat gtgatcatga tcgcgcccaa
     3061 agggccgggg catctggtgc gctcgactta tatccagggc gggggcgtgc ccagcttgat
     3121 tgcggtaaag caggacgcca gcaagaaggc gcgcgacatt gcgctttctt atgcttgcgc
     3181 catcggtgct acgcgcgccg gcgtgattga aacgacgttc cgcgaggaat gtgaaaccga
     3241 tttgttcggc gaacaggtgg tgctgtgcgg cggccttacc gcgctgattc aggccggttt
     3301 cgagacgctg gtggaagcgg gctatgcgcc ggaaatggcg tatttcgaat gcctgcacga
     3361 agtgaagctc atcgtcgacc tgatttacga aggcggcatc gccaacatgc gttactcgat
     3421 ttccaacacg gcggaatacg gcgactttac ccgtgggccg cgcatcatca acgacaagac
     3481 ccgcgccgaa atgaagaaga tcctcaagga tatccagagc gggaagtttg ccaaggaatt
     3541 cattctggaa aaccgcgccg gcacgcccac gctcaaggcc atgcgccgta tcgccgccca
     3601 gcaccccatc gagcaggtgg gcgggaaatt gcgcggcatg atgccgtgga tcaaaaagaa
     3661 caagttggtg gatcagtccc ggaattagtg aatgaggtga agaagtgaat gataaagacg
     3721 aacccaattt acctcttcgc ttgttcacct ctttacctct tcactctttc acccatgaat
     3781 tacccccatc ccctcatcgc ccgcgaaggc tggccttttc tggcggcatc agtgattgtt
     3841 tccgtgtcgg tcgttctgtt tgccggctgg ctgtggtcgg cgccgttctg gctcgcgagt
     3901 cttttcatcc tgcaattttt ccgcgatccg ccgcgcaatg tccccggcga cagctatact
     3961 gtagtatcgc cggcggacgg ccgcatcgtg gcggtggagc gctgcctaga tccttatttg
     4021 cagcgtgacg cgctcaaggt cagcgtgttc atgaatgtgt tcgacgcgca ttccaaccgc
     4081 agcccggtgg acggggtggt gaaaaaagat tggcatcatt ccggcaagtt catcaatgcg
     4141 gcgctctcaa aggcgtcagt ggataatgag cgtaaggcac tgtggcttaa aacggtaaac
     4201 ggttttgacg tgacctgcgt gcaggttgca ggattgattg ccaggcgcat actctgttac
     4261 gtcaaaagcg gcgatgcgct ggtgcgcggc cagcgctacg gcttcattcg ttttggctcg
     4321 agggtggacg tgtatttgcc gctgaatgcc gcggtgagaa ccggcatcgg cgacaaagcg
     4381 tacgcggc
//
LOCUS       DATIOW010000116         7415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1580656, whole genome shotgun sequence.
ACCESSION   DATIOW010000116 DATIOW010000000
VERSION     DATIOW010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7415
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580656"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /gene="lpxD"
                     /locus_tag="VLL03_03800"
     CDS             complement(<1..765)
                     /gene="lpxD"
                     /locus_tag="VLL03_03800"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HSS46522.1"
                     /translation="MPSGKDRGLSLGSIVKRLGGELIGDKNIIINQVAPLDSAGPGHL
                     SFLSQSKYIAQLKKTRADAVIIGHDARNLTRLARIVCDDPYAYFAKVSALLNPAKTPV
                     AGIHPSAVITKSAQVPASATIGAHACIGENVVLGERVVVGEACVVGEGVTLGEKSFLH
                     PRVVIYHDCVIGEKVILHSGTVIGADGFGMAMEEGRWLKIPQIGRVVIGDDVEIGANT
                     TIDRGSLADTHISSGVKLDNLVHVGHNCQIGEHSVVA"
     gene            complement(782..1294)
                     /locus_tag="VLL03_03805"
     CDS             complement(782..1294)
                     /locus_tag="VLL03_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HSS46523.1"
                     /translation="MKTPYLALAAALLATAAISATAAEQLKVGFVNLERVLREASPAV
                     KSLKKLEKEFAGREQELQKMAMQIKDLQASLEKEGVTMNESERRNKEQDLARLNRDFQ
                     RMQREFREDLNLRKNEELAAVLERANKVVQSIAEAEKFDLILQEAVYRSPRIDITDKV
                     IKGLSDAPSK"
     gene            complement(1317..3584)
                     /gene="bamA"
                     /locus_tag="VLL03_03810"
     CDS             complement(1317..3584)
                     /gene="bamA"
                     /locus_tag="VLL03_03810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HSS46524.1"
                     /translation="MRKIILVVLLFCLFTAAAQAIEPFVIKDIRVEGIQRTEAGTIFS
                     YLPVKVGDIMNDEKATATIKSLFATGFFKDVRCETENGVLIVIVQERPAISQIDIVGA
                     KDISADDLKKGLKQVGLAESRIFDKALLERAEQELKRQYIGRGRYAATVNTTVTPLER
                     NRVAITFSIAEGDVAKIRQINIIGNRAFPQKELLDLFTLTTPGWFTFFTKNDQYSKQK
                     LSADLETLRSFYLDRGYLEFNVDSTQVSITPNKADIYITISITEGAKYTVSEVKLGGE
                     LLLPEDEFRKLIKIQPGEVFSREKLTESTKLISDRLGNDGYAFANVNAAPELDKQKRQ
                     VAFTFFIDPGRRVYVRRINISGNTRTRDEVIRRELRQLESGWYNADLINRSKQRIDKL
                     GYFSDVNVETPAVPGTTDQVDVNYSVTEKPTGNILLGIGYSDAEGVILNAAVTQSNVF
                     GSGNYLSAQINTGNVNRVISLSFTNPYYTVDGVSLGFDIYKRNLDTTSLSVSPFKTST
                     VGGTVRFGLPISEVDTISYGLGYEDTGIEVFPTSPQRYIDFVNQFGSDNKTILGVIGF
                     TRDRRDSVIYTTSGTLFRFNAEVGLPGANLRYYKLSAQEQYYYPLSKYFTLQLGGQIG
                     IGAGYNGQPLPFFKNFFAGGTNSVRGFDTNSIGTKDNNGDALGDTHLILGSAEVLFPV
                     PGFTDIKALRLGTFMDAGTVASSFDFGSLRYSAGVDVSWTSPFGPIRFSIAQPIRKLS
                     DDKIQRFQFTFGTTF"
     gene            complement(3590..4948)
                     /gene="rseP"
                     /locus_tag="VLL03_03815"
     CDS             complement(3590..4948)
                     /gene="rseP"
                     /locus_tag="VLL03_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HSS46525.1"
                     /translation="MNLLLTLAAFALALGVLIVFHELGHYWVARLCGVKVLRFSVGFG
                     KPLWRRQLGRDGTEWVIAVFPLGGYVKMVDEQEGRVAPQDLPRAFNRQPVLKRFAIVL
                     AGPLANFLLAILLYWGLFIHGVPGLKPIIGPVQSATPAAFAGFKPEEIIVKIDGEAVA
                     TWQDVRWILLQRALQKAVVEVEARNARGEIAFRKLDLSGLASIELEGEFMNVIGLSRY
                     QPPVKPVIGKLVPGAVAERSSLQIDDEIVAINGAPVNQWEEVVQQVRSHPAQPLAFEI
                     RRAGRNLHLTLTPEAAQNEKIGKIGAAPKIDSAEIEKLFTEVRYPVAQALAKSLEKTW
                     ETSAFSLKMLGKMLVGEISWRNVSGPITIADYAGQSARIGWVAYLGFLALISISLGVL
                     NLLPVPLLDGGHLMYYLAEIIKGKPVSERALHIGQHVGIALLFILMAFALYNDINRLI
                     AG"
     gene            complement(4988..6193)
                     /gene="ispC"
                     /locus_tag="VLL03_03820"
     CDS             complement(4988..6193)
                     /gene="ispC"
                     /locus_tag="VLL03_03820"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HSS46526.1"
                     /translation="MQNLTLLGSTGSIGVNTLDVVARHPGRFRVVALSAGSSVERLFE
                     QCRRFKPVYAVLRDEKAAGQLQRMLKNTDTPTEVLQGVEALEKVATLPEVDSVMAAIV
                     GAAGLRPALAAAKAGKRVMLANKEALVMSGRLFMEAVKRNRATLLPVDSEHNAIFQCL
                     PRDFSGNLEAAGVKRILLTASGGPFRSTPLQELEQVTPDQACAHPNWVMGRKISVDSA
                     TMMNKGLEVIEAHWLFNAREDQIQVVIHPQSVIHSMVEYNDGSVLAQLGNPDMRTPIA
                     YALGYPERIEGGVQSLDLFKVGTLTFQTPDFERFPCLRLAYAALKRGDAAPAILNAAN
                     EVAVASFLEERMPYTFIPLLIEAVLEKVSVKSIASLDDVLNADCAARVAGTEWLAASR
                     IKPVTKAAG"
     gene            complement(6199..7035)
                     /locus_tag="VLL03_03825"
     CDS             complement(6199..7035)
                     /locus_tag="VLL03_03825"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HSS46527.1"
                     /translation="MLKTRVFTVALLLPLFLAALFKLPTLGWVALNLLVMTAAASEWA
                     CLARLRKPVARFFLAAVLLLLLVMVPLLDLLDEPPTSSRINLSIYALALVFWVAAAPF
                     WLRSKWQAGKLGLSLVGALVLISTWLALVQLREIDKLLILWIFSVVWVADIAAYFCGK
                     KFGQHKLAPNISPGKTWEGVGGGVLAVTAYALALVALTSNHGVGLLGNPLPAYAVLLA
                     HWVLTALSIVGDLFESLLKRQAGVKDSGTMLPGHGGMLDRIDGLTSTLPVAAFFIFVM
                     LR"
     gene            complement(7028..>7415)
                     /gene="uppS"
                     /locus_tag="VLL03_03830"
     CDS             complement(7028..>7415)
                     /gene="uppS"
                     /locus_tag="VLL03_03830"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HSS46528.1"
                     /translation="ANYGGRWDIMQAVKRMLAEHPELAEGFNEDQLAPYLAMHYAPEP
                     DLFIRTGGEQRISNFLLWQMAYTEMYFTDTLWPDFDAAALNLAIQSYQPRERRFGRTS
                     EQIKETRANPRPEIKAKSPRRARQNA"
BASE COUNT         1597 a         2152 c         2047 g         1619 t
ORIGIN      
        1 agcgacaacg gagtgctcgc cgatctggca gttgtggccg acgtgcacga gattgtcgag
       61 ttttactccc gaggagatgt gagtgtcggc gagcgagccg cgatcgatgg tggtattggc
      121 gccgatttcc acgtcgtcgc cgatgactac ccggccgatt tgcgggattt tcagccagcg
      181 cccttcctcc atggccatgc caaaaccgtc ggcgccgatc accgtgccgg aatgcagaat
      241 gaccttctcg ccgatcacgc agtcgtgata gatcacgacc cgcggatgga gaaaactttt
      301 ctcgcccagg gttacgcctt cgcccaccac gcaagcttcg cccaccacca cccgctcacc
      361 cagtacgaca ttttctccga tgcaggcgtg ggcgccaatg gtcgccgaag ctggaacttg
      421 ggcgcttttg gtaatgactg cgctgggatg gattccggcg acgggcgttt tggcggggtt
      481 gagaagcgca gaaaccttgg cgaaataagc gtagggatcg tcacaaacga tgcgcgcaag
      541 cctggtgaga ttgcgggcat cgtgcccgat aatcactgcg tccgcacggg tttttttcag
      601 ttgagcgatg tatttgcttt gcgacaaaaa cgaaagatgg cccggtccgg ccgagtcgag
      661 cggcgccacc tggttgatga taatgttctt gtcgccaatg agctcgccgc cgaggcgttt
      721 gacgatgctg ccgaggctta aacccctgtc tttgccggaa ggcattaatc ggctttgctg
      781 cttatttgga aggcgcgtcc gacagccctt tgatgacctt gtcggtgata tcaatgcgcg
      841 ggctgcgata caccgcctcc tgcaggatca ggtcaaactt ttcggcttca gcgatgcttt
      901 gcaccacctt gttggcccgt tccagcaccg cagccagttc ctcgtttttg cgcaaattga
      961 ggtcctcgcg aaattcccgc tgcatgcgct ggaaatcgcg gttgagccgg gccagatcct
     1021 gttccttgtt gcggcgttcg gactcgttca tggtcacgcc ctctttttcc agcgaagcct
     1081 ggagatcctt gatttgcatc gccatcttct gcaattcctg ttccctgccg gcgaattcct
     1141 tttccagctt cttcagtgat ttgaccgcgg gagaagcctc gcgcaatacg cgttccaggt
     1201 tgacgaagcc gactttaagt tgttcggcgg cggtcgcgct gatcgcggct gtcgccagca
     1261 gagcggctgc cagtgcaagg taaggggtct tcaacttgag tctccttcta gcaagcttag
     1321 aatgtcgttc cgaaagtaaa ctggaaacgc tgtattttat catcactgag cttccgaatc
     1381 ggctgcgcga tactgaacct gatcggcccg aacggcgagg tccaggagac gtcgacgccg
     1441 gcggaataac gcaggctgcc gaaatcaaag ctgctggcca ccgtgcccgc gtccataaag
     1501 gtccccagcc tcaatgcctt aatatccgta aaacccggca ccgggaacaa gacctccgca
     1561 cttccaagga tgaggtgcgt atcccccaac gcatcaccgt tgttatcttt ggtcccgatg
     1621 gaattggtat caaacccgcg taccgaattg gtgccgccgg cgaaaaaatt cttgaagaac
     1681 ggcagcggct ggccattgta tcctgcgcct atgccgattt gcccgcccag ctgcagcgtg
     1741 aagtacttgc tcaagggata ataatactgc tcctgggcgg aaagtttgta gtatctcagg
     1801 ttggctccgg gcaggcccac ttcggcattg aacctaaaca gcgttccgct cgtagtgtaa
     1861 atcacgctgt cgcgccggtc acgggtgaag ccgatgaccc ccagtatcgt tttgttgtcg
     1921 ctgccgaact ggttgacgaa atcgatatag cgctgcgggc tggtgggaaa tacctcaata
     1981 ccggtgtctt cgtaacccag gccataactg atggtgtcaa cctcggaaat aggcaggcca
     2041 aagcgaaccg ttccgcccac cgtagaagtc ttgaaaggag aaacggacag cgaagtagta
     2101 tcgagattac gtttataaat gtcaaaaccg aggcttaccc cgtcaacggt ataataagga
     2161 ttggtgaaag acagggatat cacccggttc acgttcccgg tattgatttg cgccgagaga
     2221 tagttgcccg agccaaaaac gttggattgc gtcaccgccg cgttaaggat aaccccttcc
     2281 gcgtcggaat aacctatgcc gagcaaaatg ttaccagtag gtttttcggt gacactgtaa
     2341 ttgacatcta cctgatcggt ggttccgggt acagccgggg tttccacgtt gacatcggaa
     2401 aaataaccca gcttgtcgat gcgctgctta gagcggttga taagatcagc gttgtaccag
     2461 ccgctttcca gctggcgcag ttcgcggcgt atgacttcat cgcgagtgcg ggtattgccg
     2521 ctgatgttga tgcgccgcac gtaaacccgc cgtcccgggt caatgaaaaa ggtaaacgcg
     2581 acctggcgtt tttgcttgtc gagttccggt gcggcgttaa cgttggcaaa cgcgtaaccg
     2641 tcattgccca agcggtcact aatgagcttg gtggattcgg tgagtttctc ccgtgaaaaa
     2701 acttcgcccg gctggatttt aatcagcttg cggaattcgt cctcgggcaa cagcaattcc
     2761 cccccgagct tcacctccga tacggtgtat ttcgcgccct cggtgatgct gatggtgatg
     2821 taaatatcgg ccttgttcgg cgtgatggac acctgggtgg aatcaacgtt gaattccaga
     2881 tatccccggt cgagataaaa tgagcgcaac gtttccagat cggcggaaag cttttgcttg
     2941 gaatactggt cattcttggt gaagaaggta aaccagccgg gcgtggtgag agtgaataaa
     3001 tccagcaatt ccttttgcgg gaaggcacgg ttgccgatga tgttaatttg ccggatcttg
     3061 gccacgtccc cttccgcgat gctgaaggta atggcgacgc ggttgcgctc gagcggtgtc
     3121 acggtggtgt tcacggtggc ggcgtaacgg cctcggccta tatattgccg cttcaattcc
     3181 tgctcggcgc gctccaagag cgctttgtcg aaaatacgcg actcggccag ccccacctgc
     3241 ttcaggcctt ttttcagatc gtcggcgctg atgtccttgg caccgacgat gtcgatctgg
     3301 gagatcgccg ggcgctcttg cacgattacg atcaacacac cgttttcagt ttcacaccgc
     3361 acgtccttga agaagccagt ggcgaacagc gatttgatgg ttgccgtggc tttttcatcg
     3421 ttcatgatat cgcccacctt gaccgggaga tagctgaaga tggtaccggc ttcggtgcgc
     3481 tggataccct ccaccctgat gtctttaatc acaaaaggct caatggcctg ggctgctgcg
     3541 gtgaaaaggc aaaacaacag caccaccagg ataatttttc tcattagttc taaccggcaa
     3601 tcaggcgatt gatatcattg tacagggcga acgccatcag gatgaacagc agggcaatcc
     3661 cgacatgctg tccgatatgc agggcccttt ccgaaactgg cttgcccttg atgatctccg
     3721 cgagataata cataagatgc ccgccgtcga gcagcggaac cggcagcaga ttgagcaccc
     3781 ccaggctgat gctgatgagc gccaggaagc cgaggtaggc aacccagccg attcgcgccg
     3841 actgcccggc gtaatcggca atggtgatgg ggccgctgac atttctccag gaaatctcgc
     3901 ccaccagcat cttgcctagc attttcagac tgaacgccga ggtttcccaa gttttttcca
     3961 gggatttggc cagcgcctgc gccaccgggt agcgcacttc ggtgaaaagt ttttcaattt
     4021 cggcactatc gattttgggc gcggcgccga tcttgccgat tttttcgttc tgcgccgcct
     4081 ccggcgtcag cgtcaagtgt agattgcgtc cggcgcgcct gatctcaaag gcaagcggct
     4141 gagccgggtg gctgcgcacc tgctgcacca cttcttccca ttggttgacg ggcgcgccgt
     4201 tgattgccac aatttcatcg tcaatctgca gactgctacg ctccgccacc gcgcccggaa
     4261 ccagcttgcc aatgacaggc tttaccggcg gctgatagcg cgacaatccg atcacattca
     4321 taaattcgcc ttccagttca atgctggcaa gcccgctcaa atcaagcttg cggaaggcga
     4381 tttcgccgcg cgcattacgc gcttctactt cgaccaccgc tttttgcagg gcccgctgca
     4441 gcaggatcca gcgcacgtcc tgccaggtag cgacagcttc accgtcgatt ttgacgataa
     4501 tttcttcggg cttgaatccg gcaaacgccg ccggtgtagc cgattgcacc gggccgatga
     4561 tgggcttcag ccccggcaca ccgtgtatga acaaccccca gtacagtaat atagccagta
     4621 gaaaattggc gagcggcccg gcgagcacaa tggcaaagcg cttgagcaca ggctggcggt
     4681 tgaacgcgcg tggcaaatcc tgcggcgcga cccgtccttc ctgctcatcc accatcttga
     4741 cgtaaccgcc cagaggaaaa acggcaatca cccattccgt gccgtcccgg cccagttgcc
     4801 ttcgccatag tggcttgcca aaacccaccg aaaagcgcag cactttcacg ccgcaaagcc
     4861 gcgccaccca atagtgtccc agttcgtgga acacgataag cacgccgagc gccagagcga
     4921 aggccgccaa tgttaataat aaattcatga atgtccgttg ttcttgatgt ccgcaaattg
     4981 catgcgctca acctgcggct ttggtgaccg gtttgatgcg tgaagcggca agccattccg
     5041 tccccgccac acgtgccgca cagtctgcgt tgagcacatc gtcaagggaa gcgatggact
     5101 ttacgctcac cttttccagc accgcttcga tcagcagcgg gataaatgtg tacggcatgc
     5161 gctcttccag gaaggaagcg accgccactt cgttcgccgc gttgagtatc gctggcgccg
     5221 catcgccgcg cttgagcgcc gcataggcca ggcgcaggca gggaaagcgc tcgaaatccg
     5281 gcgtctggaa agtcagcgtc ccgactttaa acaagtcgag cgattgcact ccgccttcaa
     5341 tccgctccgg ataacccagt gcgtaggcga tcggcgtgcg catatcggga ttgccgagtt
     5401 gcgccagcac cgagccgtca ttgtattcga ccatcgaatg aatcacgctt tgcgggtgta
     5461 ttaccacctg aatctggtcc tcgcgggcat tgaacagcca atgcgcctcg atcacttcca
     5521 gccctttgtt catcatggtc gccgaatcga ctgaaatctt gcgccccatc acccagttgg
     5581 ggtgggcgca ggcttgatcc ggggtcacct gttccagctc ttgcaacggc gtggagcgga
     5641 acggcccgcc tgaagcggtg agtagaatcc gttttacgcc cgcggcttca agattaccgc
     5701 tgaaatccct tggcaggcac tgaaaaatgg cgttgtgctc gctatccact ggcagcaacg
     5761 tagcgcggtt tcgcttaacc gcttccatga acaatcggcc cgacatcacc agagcttcct
     5821 tattggcgag catcacgcgt ttgcccgctt ttgccgccgc cagcgcgggg cgcaatccgg
     5881 ccgcgccgac aatcgccgcc atcacgctat caacttcggg cagggtggct actttctcca
     5941 gtgcttccac tccttgcagc acctcggttg gcgtatcggt gtttttgagc atgcgctgca
     6001 gctgcccggc cgctttttcg tcgcgcagca ccgcgtagac gggcttgaag cgccggcatt
     6061 gctcgaacag acgctccacg ctcgagccgg cggaaagcgc caccacgcgg aaacgccccg
     6121 ggtggcgcgc aaccacgtcc agcgtattga caccaatgct gccggtggaa ccgaggaggg
     6181 tgaggttctg aatcttcatt acctgagcat cacaaaaata aaaaaagccg cgaccggcag
     6241 agttgaggta aggccgtcta ttcgatcgag cataccaccg tgcccgggta gcatcgtgcc
     6301 gctgtcttta acaccggcct gacgcttaag cagcgattca aacaagtcgc ccacaatact
     6361 taatgcagtg agcacccagt gcgctagcag cacagcataa gccggcagcg ggttgcctag
     6421 caatccgaca ccgtgattgc tggttaaagc caccagggca agagcgtacg ccgttacggc
     6481 caaaacccca ccccccactc cttcccaggt tttgccgggg ctaatgtttg gcgcaagttt
     6541 gtgttgaccg aattttttgc cgcagaaata ggcggcgata tccgcgaccc acactacaga
     6601 gaatatccat aaaattaaca atttatctat ttcacgcaat tgcaccaagg ccagccaggt
     6661 actgatcagg accaaagctc caaccagcga cagcccaagt tttccagcct gccactttga
     6721 gcgcaaccaa aatggcgcgg cagcaaccca gaaaaccagt gccagcgcgt atatcgaaag
     6781 attaatgcgg ctgctggttg ggggttcatc cagcaagtca agcagcggca ccataaccag
     6841 cagcagcagc agcaccgctg cgaggaaaaa ccgcgccacc ggtttgcgca gacgcgccag
     6901 gcaagcccat tccgacgccg cagccgtcat caccagcaga ttgagcgcga cccaacccag
     6961 ggtgggtagt ttaaacagcg cggcaagaaa taacggcagg agcaaagcta cggtaaagac
     7021 ccgcgtctta agcattctgc cgggctcgcc gtggggattt ggctttaatt tccgggcgag
     7081 gattggcgcg cgtctctttg atttgctcac tagtgcggcc gaagcgccgt tcgcgcggtt
     7141 gataggactg gatggcaaga ttaagcgcag cggcgtcgaa atcgggccac agcgtatcgg
     7201 tgaaatacat ttccgtataa gccatttgcc acagcaggaa attgctgatg cgctgctcgc
     7261 cgccggtgcg gataaaaaga tcgggttcgg gggcataatg catggcgaga tacggcgcca
     7321 gctgatcttc attaaaaccc tcggccagct cgggatgttc cgccagcatg cgttttaccg
     7381 cctgcatgat atcccagcgg ccgccgtagt tagcc
//
LOCUS       DATIOW010000117        12835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1682250, whole genome shotgun sequence.
ACCESSION   DATIOW010000117 DATIOW010000000
VERSION     DATIOW010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12835
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682250"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VLL03_03835"
     CDS             complement(<1..806)
                     /locus_tag="VLL03_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSS46529.1"
                     /translation="MAKPNPYSTDLGKNPANYAPLSPLSFIDWAAYVYPKHVAIIYGD
                     RKITWGEMYTRCRRLASALQKRGIGVGDTVAAMLPNIPAMCEVHFGVPMAGAVLNTLN
                     TRLDAEAIAFMLKHGEAKVLITDREFSGTLAGALTLLDRKPLVIDVDDPEFKGGNLLG
                     EIEFEAFLKTGDPEFKWTLPADEWDAISLNYTSGTTGNPKGVVYHHRGAYLNALSNII
                     DWGMPRHSVYLWTLPMFHCNGWCFPWTMAANVGTNVCLRRVDARLIFDTI"
     gene            complement(891..2243)
                     /gene="radA"
                     /locus_tag="VLL03_03840"
     CDS             complement(891..2243)
                     /gene="radA"
                     /locus_tag="VLL03_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HSS46530.1"
                     /translation="MTKPKSVYSCTECGGQVTKWQGQCPHCSAWNTLVETVVEAPSKN
                     RFSSAATASKLQSLGEVEAQELPRQPTGLSEFDRVLGGGLVQGGVVLLGGDPGIGKST
                     LLLQALCHMGAQQSLLYVSGEESAQQIALRARRLALDPGPVQLLAEIQLEKILSILSS
                     QKPEVAVIDSIQTLYSEALQSAPGSVAQVRECAAQLTRYAKQSGTTIIFIGHVTKEGT
                     LAGPRVLEHIVDTVLYFEGDTHSSFRLIRAVKNRFGAVNELGVFAMTEKGLKGVNNPS
                     ALFLSQHGSQVAGSCVMVTQEGTRPLLVEIQALVDDAHAPNPRRLSVGLEQNRLAMLL
                     AVLHRHAGIMCFDQDVFVNAVGGVKIAEPGADLAVLLAIVSSLKNKPLSQKLVVFGEV
                     GLAGEIRPVQRGQERLKEAAKLGFTQAMIPKANKPKQTMNGMEIIAVERVEEAVARIR
                     "
     gene            complement(2236..3309)
                     /gene="alr"
                     /locus_tag="VLL03_03845"
     CDS             complement(2236..3309)
                     /gene="alr"
                     /locus_tag="VLL03_03845"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HSS46531.1"
                     /translation="MTRPIQARISFSALQHNLAVARQRAPDSRIMAVIKANGYGHGLL
                     RAAGALTGAEGFAVIDLNDAVILRKAGFRQCILLLNGFYSVDELPVVAENGLAVVIHN
                     KEQLEMLEAAALPVRLDVFLKLNTGMNRLGFKVEAFSGILQRLKQNRQVGSITLMTHF
                     ACADDEQGIDAQLKQFNKIAAGQNLPKSLANSAAVLRYPEAHANWVRPGIMLYGSSPF
                     AEKSAKELGLQPVMTLTSRIIAMQDLQPGERVGYTGLFTAAKPMKVGIVACGYADGYP
                     RHARTGTPVNVSGKITRTLGRISMDTLGVDLSEIPEAGVDAPVTLWGEGLPVEDVAKA
                     AGTVSYELFTKLNSRVPVVEIND"
     gene            3495..4727
                     /gene="lplT"
                     /locus_tag="VLL03_03850"
     CDS             3495..4727
                     /gene="lplT"
                     /locus_tag="VLL03_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HSS46532.1"
                     /translation="MNRGFYTILAAQFFSALADNALLFAAIGLLMELQAPEWQTPVLQ
                     QFFVFSYIVLAPFVGPFADALPKGKVMFISNAVKFAGCLFMLIGSHPLYAYGIVGVGA
                     AMYSPAKYGILTEYLPPQKLVLANGWMEGLTVVAIILGAISGGILVKPELANKYLEMF
                     DIPGIETGINTPPEFAIAVILGFYFAAAIINLYIPRVALDHKLPRKDPLYILHDFWHC
                     FKLLWRDPLGQVSLAVTTLFWGVGATLRLIVLAWAAVALNFGLDKATQLTAVVAVGIA
                     IGSVIAAKYVKLEKSVRILPVGVAMGLSLMLMIVVSNLYIAIAMLLVIGAFTGFFIVP
                     MNALLQHRGHLLMGAGHSIAVQNFNENLSILAMLGVYAVMIRLEFSVFTVMISFGIFI
                     SVAMAYLYRRHAQARIET"
     gene            complement(4724..5221)
                     /locus_tag="VLL03_03855"
     CDS             complement(4724..5221)
                     /locus_tag="VLL03_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HSS46533.1"
                     /translation="MNLKRIFKHLVIGHFAVRRAFPPRALRAIEQAIRDCEAAHRGQI
                     RFAVESALDIAALSRGVSARARAIEVFSQLRVWDTEHNNGVLIYLLLADRNVEIVADR
                     GVHARLGNSGWEEICRTMEQAFRERRFEEGVIAGIKAVGGHLQRHYPSQPPPPNELPD
                     KPVIL"
     gene            complement(5224..6072)
                     /locus_tag="VLL03_03860"
     CDS             complement(5224..6072)
                     /locus_tag="VLL03_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006024277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HSS46534.1"
                     /translation="MIRAWATFFSLFFLGAGLASAEVAIPPLQARVTDLTHTLSGGEI
                     RQLEEKLAAFEAKKGSQIAVLVVPTTQPETIEQYGIRVAGAWKLGRKGIDDGALLLVA
                     KEDRTLRIEVGYGLEGALNDATAKRIISEIVVPHFKRGDFYGGIDAGVSRMMAVIEGE
                     PLPQPKRPSSSSGFGNIESLFFVGFMLVVMVGGMLRALFGRFAAATIIGMAAGFLAWL
                     IIAPLVVAIIISIIGFVFTLLGGGRGWYGGPGGYSGGGFGGGFSGGGFSGGGGGFGGG
                     GASGSW"
     gene            complement(6069..6671)
                     /locus_tag="VLL03_03865"
     CDS             complement(6069..6671)
                     /locus_tag="VLL03_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HSS46535.1"
                     /translation="MRKLPVYLFLAIAALALSGCGYNTLQSTDEQIKASWAEVLNQYQ
                     RRADLVPNLVNTVKGYAQQEKDVLLGVTAARSKVGAIQATPELINDPQAFAKFQQAQG
                     ELTSALSRLLVVAENYPQLKSDANFRDLQAQLEGTENRITVARNRYIKAVQEYNVTVR
                     SFPSNLTAMLFGFQLKPTFTVENEKEIAKPPRVDFSAPNK"
     gene            complement(6728..7279)
                     /locus_tag="VLL03_03870"
                     /pseudo
     CDS             complement(6728..7279)
                     /locus_tag="VLL03_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006407727.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
     gene            complement(7402..7863)
                     /gene="rimI"
                     /locus_tag="VLL03_03875"
     CDS             complement(7402..7863)
                     /gene="rimI"
                     /locus_tag="VLL03_03875"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HSS46536.1"
                     /translation="MSAQLKEVPQLRPMRSPDLDAVIRIEGKIYSHPWTRGNFQDSLN
                     AGYSCWIYELAEQVIGYAVMMVAAEEAHLLNLSVAAEWQRQGWGKKFLKHLVGVARCY
                     HAKVLLLEVRPTNAAGLKLYQGYGFRQIALRRGYYPASDGREDAVLLEFKL"
     gene            complement(7860..8531)
                     /gene="tsaB"
                     /locus_tag="VLL03_03880"
     CDS             complement(7860..8531)
                     /gene="tsaB"
                     /locus_tag="VLL03_03880"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HSS46537.1"
                     /translation="MNILALDTSGDYCSLALWLNGEMVSREEPAEQNHSEVLLPLLDA
                     FLRENRVSLDELHGIAFGAGPGSFTGLRIACGVAQGLAFAKDLPVAGICTLKALAEAM
                     PGEKIIACLDARMGEIYHAVYEKSGDSWQAASEPGLCTPQQAPLVSGDGWIGCGSGFA
                     VHGEKLRQRYHGHLTQVAADAVPRAREMARLAVSVLERGEGCKAEEAAPLYLRNKVAL
                     KTFER"
     gene            complement(8662..11424)
                     /gene="mgtA"
                     /locus_tag="VLL03_03885"
     CDS             complement(8662..11424)
                     /gene="mgtA"
                     /locus_tag="VLL03_03885"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HSS46538.1"
                     /translation="MKSELTKDNWLRRLFLPRREKARLAISKTIESKYAKPDVTAASH
                     SRNWLIEAAHNDILNVLGSVHSSPSGLGQDEAQSRLKEHGLNEVAHEQPATWYRQLWH
                     AYNNPFNLLLTGLAVVSFITEDIKATTILSVMVVLSSLLRFVQEYRSSKAAEKLKAMV
                     STTATVRRTKETYTNAHNSSQPILVAAYPRGFETQEIPLKYLVPGDVVHLSAGDMIPA
                     DVRLLLAKDLFISQAALTGESFPVEKNADTVVGDTRNPLDLPNICFMGSNVVSGTAIA
                     VVVATGGQTYFGGLSQRIVGHRVPTEFDKGIHRVSWLLIRFMAVMAALVFLINGITKG
                     NWTEASLFAIAVAVGLTPEMLPMIITATLAKGAVMMSRRKVIVKRLNSIQSFGAMDIL
                     CADKTGTLTQDRIILVKYVDVNGDESEEVLRHAFLNSHYQTGLKNLLDKEVLRHAEIA
                     DVLKIATNYSLVDEIPFDFVRRRMSVVVNEREDHNELICKGAVEEVLKVSSQAKVNGH
                     VVPLNGDLETNVMKLVKEFNEDGLRVIAVAYKETPPHQKSYGIKDESDLTLLGFIAFL
                     DPPKETAGPAITALKQNGVQIKILTGDNEVVTCKICRDVGIDAGRVVLGSEIEDLSDA
                     ELAELAERAAVFAKLSPAQKERIVRVLQLKGHVVGFLGDGFNDSAALRTADIGISVDT
                     AVDIAKESADIILLEKHLMVLEEGVLEGRKTFGNIVKYIKMAASSNFGNMFSVLGAST
                     FLPFLPMLPIQLLIQNLLYDISQIAIPFDSVDKEYSERPRKWEIGALGRFMVFIGPIS
                     SVFDYATFAVMWFAFSANTIEVQSLFQSGWFIEGLLSQTLIVHIIRTGKIPFIQSRAT
                     LPLLLMTASVMAIGIYIPFSPIAHVVGLRPLPGFYFAWLAGILFGYTVLTQFVKVWFI
                     RKYGFN"
     gene            complement(11480..>12835)
                     /locus_tag="VLL03_03890"
     CDS             complement(11480..>12835)
                     /locus_tag="VLL03_03890"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HSS46539.1"
                     /translation="VKKMRKTTEQYLYTALVAVMLLTIVMVANAYAEGPEVPQFLGAQ
                     YTLIAQHLFPFEAAYSGPNSLRNDGDTQKTQTFGLYSGMKVWNHLQAYLDFELFKGAA
                     VSNATGLGGLTNGDVLRQGSFNIGKRPYVARAFLRYFWPMGDEMIDPAVRAMDQLPGV
                     EPAKRIEVKLGKFAANDDFDRNRYANSTRTQFENWSLWNNTAWDYAADTRGYTNGVMV
                     GYVSPIWALRLGIYQMPTVANGPDLDQPLTKSRGENLELTLQPNHYGTAVRLLAYRNV
                     ANMGIYRDAIAIGLAQGAVPNVAADDQPGRKKYGFGLNLEQPLADGGETGLFARLGWD
                     DGKTESFAFAEVDRTVTFGAQVAGNHWRRSADRLGIALAVNGLSADHRHYLAAGGSGF
                     VLGDGRLNYGYEKIVEAYYRIQLGKYAQLSPDFQYIDNPGYNRDRGPATVVAIRLHIE
                     Y"
BASE COUNT         2742 a         3826 c         3433 g         2834 t
ORIGIN      
        1 ttgatggtat cgaatatcag cctggcatcc acccgccgca ggcagacatt ggtgcccacg
       61 ttcgccgcca tggtccaggg aaaacaccaa ccgttgcagt ggaacatcgg cagcgtccac
      121 agataaaccg aatggcgcgg catgccccag tcgatgatgt tgctcaacgc attgagatac
      181 gcgccgcgat ggtggtagac cacgcctttg ggattgccgg tcgtgccgga ggtgtaattg
      241 agcgaaatcg cgtcccattc gtcggcgggc agtgtccatt tgaattccgg atcgccggtc
      301 ttgagaaacg cttcgaattc gatttcaccg agcaaattgc cgcctttgaa ttccggatcg
      361 tccacgtcta tcaccagcgg cttccggtcc agcagggtga gcgccccggc aagcgtcccg
      421 gaaaattcgc ggtcggtgat caataccttc gcttcgccgt gcttgagcat gaaagcaatc
      481 gcttccgcat ccagccgggt attcagggtg ttcagcaccg cgccggccat cggcacgccg
      541 aaatgaactt cgcacatcgc ggggatgttc ggcagcatcg ccgccaccgt gtcgcccacg
      601 ccgataccgc gcttttgcaa agcggaagca aggcgccggc agcgggtata catttcaccc
      661 caggtaattt tccggtcgcc gtaaatgatc gccacatgct tcgggtaaac ataagccgcc
      721 caatcgatga aactcaacgg cgaaagcggc gcatagtttg cgggattttt gcccaaatcg
      781 gtactgtagg gattgggttt agccatggca tcctcttatc gctgcacaag caatccgtct
      841 gctgtcaaga aatgaattta cccgctcgcc ccggcaatgg caacccgctg ctaacggatt
      901 ctggctacag cttcttccac gcgctccact gcgatgattt ccattccatt cattgtctgc
      961 ttgggcttgt tggctttggg aatcatcgcc tgggtgaagc cgagcttggc ggcttccttg
     1021 aggcgctcct gcccgcgctg caccgggcgg atttcgccgg cgagtccgac ttcgccgaac
     1081 accaccagct tttgcgacag cggcttgttc ttgagcgagg aaacaatcgc cagcagcacc
     1141 gccagatcgg cgccgggttc ggcgattttg actccgccca ccgcgttgac gaacacatcc
     1201 tggtcgaaac acatgattcc cgcatggcgg tgcaacaccg cgagcagcat cgccagccga
     1261 ttctgctcca gccccacgct gagccgccgc ggattgggtg cgtgcgcatc atccaccagc
     1321 gcctggattt ccaccaacag cgggcgcgtg ccttcctgcg ttaccatgac gcaagagccc
     1381 gccacttggc tcccgtgttg cgaaaggaac agtgcggaag ggttgttcac tcctttcaag
     1441 cctttttcag tcatggcgaa cacgcccaac tcgttcaccg cgccgaagcg gtttttcacc
     1501 gcgcgaatca aacggaagct ggaatgcgtg tcgccctcga aatacaacac ggtgtccacg
     1561 atgtgctcaa gcacgcgcgg gccggcgagc gtaccttcct tggtgacatg gccgatgaaa
     1621 ataatcgtgg tgccgctttg cttggcgtag cgcgtaagct gtgccgcgca ttcgcgcacc
     1681 tgcgccaccg agccgggcgc cgattgcaat gcttcggaat ataaagtctg aatcgagtcg
     1741 atgaccgcga cttccggttt ttgcgagctt aagatggaaa gaattttttc aagctggatt
     1801 tcggcgagca gttgcaccgg cccgggatcg agcgccagcc ggcgcgcgcg cagtgcaatc
     1861 tgttgcgccg attcctcgcc gctcacgtaa agcaagctct gctgcgcgcc catgtggcac
     1921 agcgcctgca gcagcagcgt ggacttgccg atgcccggat cgccgccgag caataccact
     1981 ccgccctgca ccagcccgcc tcccagtacg cggtcgaatt cggacaagcc tgtgggctgg
     2041 cgcggcaact cctgcgcctc gacttcaccg agcgattgca acttgctggc agttgcagcc
     2101 gagctgaagc ggtttttgga aggcgcttca accaccgtct cgaccagcgt gttccatgcc
     2161 gagcagtgcg gacattgccc ctgccatttg gtcacctgcc cgccacattc ggtgcagcta
     2221 tagacggatt tgggtttagt cattgatttc aaccacaggc acgcgcgaat tgagcttggt
     2281 gaaaagttcg taactcactg ttcccgccgc tttggccacg tcctctaccg gcaatccttc
     2341 accccacagc gtgaccggcg cgtcgacacc tgcttcaggg atttcgctca aatccacgcc
     2401 aagcgtgtcc atggaaatgc gcccgagcgt gcgcgtgatt ttgccgctca cattcaccgg
     2461 cgtgccggtg cgcgcgtggc gcggatagcc gtcggcataa ccgcacgcaa cgatgcccac
     2521 tttcatcggc ttcgccgcgg tgaaaagccc cgtgtaaccg acacgctcgc ccggctgcaa
     2581 atcctgcatg gcgattatcc ggctggtgag cgtcatcacc ggctgcaagc ccagctcctt
     2641 tgcgctcttt tcggcaaacg gcgaggaacc gtacaacata atgcccggac gcacccaatt
     2701 ggcgtgagct tcggggtaac gcaacaccgc cgcggaattg gcgaggcttt tcggcaaatt
     2761 ctggccagcc gcaattttgt tgaattgctt aagctgcgca tctatgccct gctcgtcgtc
     2821 ggcacaggca aaatgcgtca tcagcgtaat acttcccacc tgccggttct gcttgagccg
     2881 ctgcaggatg ccgctgaatg cttcaacctt aaacccgaga cggttcatgc cggtattaag
     2941 cttcaggaac acatcaagcc tgaccggcag cgcggcggct tccagcatct ccaactgctc
     3001 tttattatgg atcaccactg ccaggccgtt ttcagcaacc accggcagct catcgacact
     3061 gtagaatccg ttaagtaaaa gtatgcattg gcgaaagccg gccttgcgca agatcaccgc
     3121 atcgttgagg tcgatcaccg caaagccttc ggcgcctgtc agcgctccgg ccgcgcgcag
     3181 caggccgtgg ccgtagccgt tggccttgat caccgccatg atgcgcgaat ccggcgcgcg
     3241 ttgccttgcc acggcgaggt tgtgttgcaa ggcggaaaag ctgatgcggg cctgaatcgg
     3301 gcgggtcatg tctgtttttt aggcagcagc aactaaaagg gggcagcgtg ttattcaaac
     3361 acaaaacgcc tggtgagggt agtgtcagaa cggatctgaa tgcatggcgg gcagtttcat
     3421 ggtataaagc ttccggaacc aaacgataaa atgtctgttc acataacaat tacatttatc
     3481 aatcccccca aaggatgaat cgcggtttct acaccattct agcggcgcag tttttttccg
     3541 cgcttgccga taacgcattg ttgtttgccg cgattggcct gctgatggag ctccaggcgc
     3601 cggagtggca aaccccggta ctacagcagt tcttcgtgtt ttcctacatt gtgctggcgc
     3661 cgtttgtcgg cccgttcgcc gacgcgcttc ccaagggcaa ggtgatgttc atcagcaatg
     3721 cggtaaaatt cgcgggctgt ctgttcatgc tgatcggctc gcacccactt tacgcctacg
     3781 gtattgtcgg cgtcggcgcg gcgatgtact cgccggcgaa atacggcatt ctcaccgaat
     3841 acctgccgcc gcaaaaactg gtgctggcga acggctggat ggagggtctc accgtcgtcg
     3901 ccattattct cggcgccatt tccggtggta ttctggtcaa accggaactc gccaataaat
     3961 atcttgaaat gttcgacatc ccgggtatcg aaaccggcat caacacaccg cccgaattcg
     4021 ccattgcggt aattctcggc ttttactttg ccgccgccat catcaacctt tacattccca
     4081 gggtcgcgct tgaccacaag ctgccgagga aagatccgtt gtatatcctg cacgacttct
     4141 ggcactgctt taagctattg tggcgcgatc cgctcggaca ggtatcgctg gcggtcacca
     4201 ccttgttctg gggcgtgggc gcgacgctgc gcctgattgt ccttgcctgg gcggcggtgg
     4261 cgcttaattt cgggctggac aaagccacac agcttaccgc cgtggtggcg gttggtatcg
     4321 ccatcggctc ggtcatcgcc gcgaaatatg tgaagctgga aaaatcggtg cgtatcctgc
     4381 ccgtcggcgt cgccatgggc ttgagcctga tgctgatgat tgttgtcagc aacctgtata
     4441 ttgccatcgc catgctgctc gtcatcggcg cgtttaccgg cttctttatc gtgccgatga
     4501 acgccctgct gcagcatcgc gggcatttgc tgatgggcgc cggccactcg attgcagtgc
     4561 agaatttcaa cgaaaacctg agcattctcg ccatgctcgg cgtttacgcg gtgatgatca
     4621 ggctggaatt ctcggtgttt acggtcatga tttcgttcgg catcttcatc agcgtggcga
     4681 tggcctacct ttaccgcagg cacgcgcagg ccagaatcga aacttagagt ataactggtt
     4741 tatccggcag ctcattgggt ggcggcggct gcgagggata atgccgttgc aagtggccgc
     4801 ccaccgcctt gataccggct atcactcctt cttcaaagcg ccgctcccga aaggcctgct
     4861 ccattgtgcg gcaaatttct tcccagcccg aatttcccag cctggcatga acgccgcggt
     4921 cggcgacgat ttccacattg cggtcggcaa gcagcagata aatcaacacg ccgttgttgt
     4981 gctcggtgtc ccacacccga agctgcgaaa atacttcgat ggcgcgcgct ctggcgctta
     5041 ccccacgcga cagcgctgca atatccagcg cggattcgac cgcaaagcgg atctgtcctc
     5101 gatgagcggc ttcacaatcg cgaatcgcct gctcgatggc ccgcagcgcg cgcggcggaa
     5161 acgcccgcct gaccgcgaag tgcccgatga caagatgttt gaagatgcgc ttaagattca
     5221 tggtcaccaa ctgccggaag cgccgccgcc gccgaaaccg cccccgccgc cactgaaacc
     5281 gccaccgctg aagccgcctc cgaaaccgcc gccgctgtaa ccgcctggcc caccgtacca
     5341 accgcggcca ccacccaata atgtaaacac aaaaccaata atgctgatga taatggcaac
     5401 gaccaatggc gctattatca gccaagcgag aaatcccgca gccattccaa taatggtcgc
     5461 ggcggcaaaa cgcccgaaca aagcacgcaa catgccgccg accatcacga ccagcatgaa
     5521 gccaacaaag aacagcgatt cgatattgcc gaaaccggat gaagatgaag gacgtttagg
     5581 ttggggcagc ggctcgcctt caatcaccgc catcatccgc gaaacgccgg cgtcgatgcc
     5641 gccgtagaaa tcgccgcgct tgaaatgcgg cacgacgatt tcgctgatga tgcgcttcgc
     5701 cgttgcatcg ttgagcgcgc cttcgaggcc gtagccgacc tcgatgcgca aggtgcggtc
     5761 ttccttcgcc acgagcagca gcgcgccgtc atctattccc ttgcgtccca gcttccatgc
     5821 ccccgccacg cggatgccat attgctctat ggtttcaggt tgtgtggtgg gaacgacgag
     5881 gaccgcaatc tggctgccct ttttggcttc aaaagcggcg agcttctctt ccagctgccg
     5941 gatttccccg cccgagagag tgtgggtgag atcggtgacg cgcgcctgga gcggcgggat
     6001 cgccacctcg gcgctggcga gccccgcgcc aagaaaaaac aacgaaaaaa atgtggccca
     6061 cgcgcggatc atttattagg cgcggaaaaa tcgaccctgg gcggcttggc gatttccttt
     6121 tcgttttcga cagtaaacgt gggcttgagc tggaagccga acagcatcgc ggtcagattg
     6181 ctgggaaacg atcgcaccgt gacgttgtat tcctgcaccg ccttgatgta gcggttgcgc
     6241 gcgacggtga tgcggttttc cgtgccttca agctgcgctt gaagatcgcg gaaattggcg
     6301 tcggacttga gctgaggata attttccgcc accaccaaaa gccgcgacag cgcagaagtg
     6361 agttcgccct gcgcctgctg gaatttggcg aatgcctggg gatcgttgat gagttcgggc
     6421 gtcgcctgaa tcgcgcccac tttggaacgc gccgcggtca caccgagcag cacgtctttt
     6481 tcctgctgcg cgtaaccctt caccgtattc accagattgg gcacaagatc ggcgcgccgc
     6541 tgatactggt tgagcacttc cgcccagctt gccttgatct gctcgtcggt ggactgcaag
     6601 gtgttgtagc cgcagccgct taaagccagg gcggcaatgg ccaagaacag gtaaaccggt
     6661 aatttgcgca tggagatctc ctgtttggga taaagctttg acccctacat gggggcacaa
     6721 ccggcgatta caactcccgc atggtcttgc gggcaaagca caaatcggcc caggccttgg
     6781 ccttgtcggg cagattgcgc agcagatacg cgggatgata ggtcacgatg agcgggatgt
     6841 cacgatagct gtgcaaacgc ccgcgcaagc tggaaatggt ggcgtctgtt ttcagcagat
     6901 tttgtaccgc gactttgccc agagcgacaa tcagcctggg ctcgatcaag gcgatttgcc
     6961 gctcgagata aggctcgcat tgttgcgctt catgcggctc cggattgcgg tttcccggcg
     7021 ggcggcattt cacgacgttg gcgatgtaca cattgtcgcc gcgtctcagg ctgatcgccg
     7081 ccagcatgct gtcgagcagt ttccccgcct ggccgacaaa cggctcgcct ttggcgtctt
     7141 cttccgctcc cggcccttcg cctatgaaca gccagtcggc gttttcatca ccgacgccga
     7201 aaacggactg cgtgcgcttt tcatgcaagg ggcagtcttt gcaaacggca acgcaagctt
     7261 tgagttcctg ccaatccatt tgcaaaatcc gcgcgaggcg gtcgtccgcg gcgcccgctg
     7321 cgacgctggt ttcattcgcg gcgcggagcc gccactgcgg ccacaggccc agctctttca
     7381 ggattttctc gcgccgcccg ctcacagctt gaactccagc agcaccgcgt cctcgcgtcc
     7441 atcagaagcg ggataatagc cgcggcgcag cgcaatttgc ctgaagccat aaccttggta
     7501 aagcttgagc cctgcggcat tggtggggcg gacttccaat agcagcactt tggcatggta
     7561 gcagcgcgca acgccgacga ggtgtttcag gaattttttg ccccaaccct ggcgctgcca
     7621 ctccgcggca acgctcaagt tgagaagatg tgcttcttcc gccgccacca tcatcaccgc
     7681 ataaccgatg acttgttccg ccagctcgta aatccagcag ctgtagccgg cgttcagcga
     7741 atcctggaaa ttgccgcggg tccaggggtg ggaatagatt ttcccctcga tgcgtatcac
     7801 cgcgtcgagg tcggggctcc gcatcgggcg cagctgcggc acttctttca gttgcgcgct
     7861 catcgctcga aagtcttcaa cgccacttta ttgcgcaaat aaagaggcgc ggcttcctcc
     7921 gccttgcagc cctcgccccg ttcaagcacg gaaaccgcga gtcgcgccat ttcccttgcg
     7981 cgcggcacgg catccgccgc aacttgagtt aaatgtccat gataacgctg ccgcagcttt
     8041 tctccatgaa ccgcgaagcc gctgccgcaa ccaatccagc catcgcccga gaccagtggt
     8101 gcctgttgcg gcgtgcacag gcccggctcg cttgccgcct gccaggaatc accgctcttt
     8161 tcgtacacgg cgtggtagat ttcgcccatg cgcgcgtcca ggcaggcgat gattttttcg
     8221 cccggcatgg cttcagccag cgctttcagt gtgcagatcc ctgccaccgg caagtccttt
     8281 gcaaacgcca gtccctgcgc tactccgcag gcgatgcgca ggccggtaaa tgatcccggc
     8341 ccggcgccaa aagcgatgcc atgtagctca tccagcgaaa cacggttctc gcgcaggaac
     8401 gcatcaagca gcggcagcaa tacttcggaa tgattttgct cagccggctc ctcgcgcgac
     8461 accatttcgc cgttcagcca cagggcaagc gagcaataat cgccggaggt gtcgagcgcg
     8521 aggatgttca tgaggcaccg ttacgccatt ttacacgcaa actcgcctcg tcctcacact
     8581 ccggaagtca gggaatccca agcgcgcggc tcatttgacg agcgtgacaa agaacgacaa
     8641 tgccgtaacg atcatcaacc gctagttaaa gccatatttg cgtataaacc aaaccttgac
     8701 gaactgcgtt agaacagtgt aaccaaaaag tattcccgca agccaagcaa aataaaaacc
     8761 cggcagcggc cgcaggccca caacatgtgc gatgggagaa aacgggatat agatacctat
     8821 tgccatcaca cttgcggtca tgagcaagag cggcaacgtt gcccggcttt gtataaatgg
     8881 aatctttccg gtgcgtataa tgtgcacgat cagggtctgc gacaacaagc cctcgataaa
     8941 ccaaccggat tgaaaaagag attgcacctc tatcgtattg gctgaaaatg cgaaccacat
     9001 cacggcaaaa gtcgcataat caaaaacaga actgatcggg ccgatgaata ccataaaacg
     9061 tccgagggcg ccgatttccc acttgcgcgg ccgctccgaa tattccttat ctacactgtc
     9121 gaacggtatg gcaatctgcg agatgtcata aagcagattc tgtatcaaca actggattgg
     9181 caacatgggc aagaaaggca gaaatgtgct tgcgccaagc acgctgaaca tattcccgaa
     9241 gtttgagctt gccgccatct tgatgtattt cacgatgttg ccgaacgttt tccgcccctc
     9301 cagcacgcct tcctcaagca ccatcaggtg cttttccagc aaaattatgt ctgcggattc
     9361 ttttgcgatg tccactgcgg tgtcaaccga aatgcctata tctgcagtac gaagagcggc
     9421 agaatcatta aaaccatccc ccaggaaacc caccacatgg cctttcagct gtaaaaccct
     9481 aacaatccgc tctttttgag ccggagaaag cttggcaaat accgccgccc tctcggccag
     9541 ctcggccagc tcggcgtcgc tcaaatcctc gatttcgctg ccaagtacaa cacggcccgc
     9601 gtcaatgcca acatcacggc atattttgca agttacaact tcattatctc cggttaggat
     9661 tttgatctga actccatttt gtttcagcgc ggtaattgcc ggtcctgcag tttccttagg
     9721 gggatcgaga aacgcaataa agccaagcag tgtcaaatca gattcgtctt tgatgccata
     9781 actcttctga tgcggcggtg tctccttgta ggcgaccgca ataacacgca ggccatcctc
     9841 gttaaactct ttaacaagct tcataacgtt tgtttccaaa tcaccgttca acggaaccac
     9901 gtgtccgttt accttggcct ggctgcttac tttaagaact tcctcgaccg cacctttaca
     9961 gatgagttcg ttgtggtcct cgcgctcatt gacaactact gacatacgac gacgcacgaa
    10021 atcgaaaggg atttcatcca caaggctgta gttggtcgca attttcagca catcagcgat
    10081 ttcagcgtgc cggagaacct ctttatccaa caagtttttc aggccggttt gataatggct
    10141 attgaggaag gcgtgtcgca gcacctcttc actttcgtct ccatttacat ctacgtactt
    10201 caccagaatg attctgtctt gggttaacgt tcctgttttg tccgcacaca ggatatccat
    10261 tgcaccaaag ctttgaatcg aattgaggcg cttcacaatc acctttcggc gagacatcat
    10321 gaccgcacct ttggcaagtg tggcggtgat aatcatcggg agcatttccg gtgtaagtcc
    10381 gacagcaact gcgatcgcaa aaagcgacgc ttctgtccag tttcccttag tgatgccgtt
    10441 gatcaggaac acaagagccg ccattaccgc cataaagcga atcagcaacc aactgactcg
    10501 atgtatgcct ttatcaaact cggttggcac gcgatgacca acgatccgct gtgacaaccc
    10561 gccaaaataa gtctgaccgc ctgtggcaac aacgacggct atcgcagtac cgctcaccac
    10621 gttcgagccc atgaagcaga tgttaggcaa atccagcggg tttcttgtgt ccccaacaac
    10681 ggtgtccgca ttcttttcga ccggaaacga ttcgcctgta agcgccgcct ggctgatgaa
    10741 caaatccttt gccaacagca gtcggacgtc tgccggtatc atgtcgccag cagacaggtg
    10801 cacgacatcg ccgggcacca gatatttaag agggatctcc tgcgtttcaa aaccacgagg
    10861 ataagcagca acaagtattg gttgtgacga gttgtgggcg tttgtgtacg tttccttagt
    10921 gcgccgcacc gtggccgttg tgctgaccat ggcctttagt ttctcagctg ccttgcttga
    10981 ccggtactcc tggacaaaac gaagcaagga gcttagcacc accattaccg aaagaatcgt
    11041 ggtagctttg atgtcttctg ttataaatga aaccactgca agtcccgtaa gcaacagatt
    11101 gaacggattg ttgtacgcgt gccagagttg ccgataccaa gtggctggtt gttcatgagc
    11161 cacttcgttg agcccgtgct ccttgagccg cgattgcgcc tcatcctgac ccaaaccgct
    11221 cggggacgaa tgtactgagc caagaacgtt gagaatgtcg ttatgcgccg cctcaataag
    11281 ccagtttctc gaatgactgg ccgcggttac atcaggtttg gcatatttgc tttcaatcgt
    11341 tttcgagatc gccagacgcg ctttctccct gcgtggcaga aacagccgcc taagccagtt
    11401 atcttttgtc agttcggatt tcattctcat tcccttctgc caattgtaat tacaccatta
    11461 ttcaaacagc caagccgatt taatattcga tatgcaggcg aattgccaca actgttgccg
    11521 gcccgcggtc gcggttatag ccggggttat cgatgtattg gaaatcgggg cttagctgag
    11581 cgtacttgcc cagctggatg cggtaatagg cctcgacaat cttctcgtag ccgtagttga
    11641 gacgaccatc cccaagtaca aagccgctgc cgccagcggc aaggtagtgg cgatgatcgg
    11701 cggacagccc gttcaccgcc aacgcgatac ccaatcgatc ggcgctacgc ctccagtgat
    11761 tgcccgccac ctgtgccccg aacgtcaccg ttcgatccac ctcggcgaag gcaaagcttt
    11821 cggtcttccc gtcgtcccag cccaatcggg cgaacaggcc ggtttcccca ccatcggcaa
    11881 ggggttgctc caagttcaga ccaaatccat atttcttacg cccaggctgg tcgtcagcgg
    11941 cgacgttggg cacggccccc tgcgcgaggc caattgcgat ggcgtcgcga tagatcccca
    12001 tattggcaac gttgcggtaa gcaaggagcc gcaccgcggt tccgtagtga ttgggctgca
    12061 gggtaagttc cagattttcg ccgcgggact tggttaaagg ctgatcgagg tccggcccgt
    12121 tcgcgactgt aggcatttga taaatgccga gcctcagtgc ccagatcggt gagacgtagc
    12181 ccaccatgac accattggtg tagccccgtg tatcggccgc gtagtcccag gccgtattgt
    12241 tccaaaggct ccagttttca aactgggtac gggtcgaatt ggcgtagcgg ttgcggtcaa
    12301 agtcatcatt ggccgcaaat ttaccgagct tgacttcgat cctctttgcc ggctcgacac
    12361 cgggtaactg atccatcgct cgaacggcgg ggtcaatcat ctcgtccccc atgggccaga
    12421 agtaacgtaa gaatgcccgg gcaacatacg gtctcttgcc aatattgaat gagccctggc
    12481 gaagaacatc accattagtt aatcccccca gaccggttgc attgctgact gcggctcctt
    12541 taaagagttc aaaatcaaga tacgcctgca gatggttcca gaccttcatg ccggaataca
    12601 ggccgaaagt ctgggttttt tgtgtgtcac catcattacg taaactgttg gggcccgagt
    12661 aagcggcctc aaagggaaag agatgttggg caatcagcgt atattgggcg cccagaaact
    12721 gcgggacctc tgggccttcc gcatacgcgt tggccaccat gactatggtg aggagcatta
    12781 ccgccacgag cgccgtgtag aggtattgct ctgtggtttt tctcatcttt ttcac
//
LOCUS       DATIOW010000118         9653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1173944, whole genome shotgun sequence.
ACCESSION   DATIOW010000118 DATIOW010000000
VERSION     DATIOW010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9653
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173944"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1938)
                     /gene="rpoC"
                     /locus_tag="VLL03_03895"
     CDS             complement(<1..1938)
                     /gene="rpoC"
                     /locus_tag="VLL03_03895"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HSS46540.1"
                     /translation="MKALLDLFKQVTQEEEFDAIKIGLASPEKIRSWSYGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPVTDYECLCGKYKRLKHRGVICEKCGVEVTLSKVRRERM
                     GHIELASPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAYVVTDPGMTPLTRCQLL
                     SEDDYLAKVEEFGDEFSASMGAEGIRALLRNLNMNQEIENLRKELAATGSDSKIKKLA
                     KRLKVLEAFHQSGIKPDWMIMEVLPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLELKAPEIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRALKSLADMIKGK
                     GGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKKMALELFKPFIFHKLEVLGLSA
                     TIKAAKRLVEAEVPEVWDILEEVIREHPVMLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAFNADFDGDQMAVHVPLSLEAQMEVRTLMLSTNNVLSPSNGEPIIVPSQDIVL
                     GLYYTTREKINAWGEGMQFTDVAEVSRAYDSRQVELNARISVRIKEYEKDAGSEFKEK
                     ITRHDTTVGRALLSDILPKGLPFDLINKPLKKKEISKLINASFRRCGLRETVIFADKL
                     MYSGFTLASKAGISICVDDMQV"
     gene            complement(2078..6151)
                     /gene="rpoB"
                     /locus_tag="VLL03_03900"
     CDS             complement(2078..6151)
                     /gene="rpoB"
                     /locus_tag="VLL03_03900"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HSS46541.1"
                     /translation="MSYSFTEKKRIRKSFAKRASVLPVPFLLATQLDSYASFLQAEVP
                     PEKRKNEGLQAAFTSIYPIESHSKNARLDFVSYALGTPPFDVKECQQRGLTFAAPLRA
                     KVRLTIMDREASKPTVKEVKEQEVYMGEIPLMTLTGSFVINGTERVIVSQLHRSPGVF
                     FEHDRGKTHSSGKLLFSARVIPYRGSWLDFEFDPKDYLYFRVDRRRKMPVTTLLKAIG
                     MTPEQILAEFFAFDTFHISKKGIQFELVPERMRGEVARFDITTRGGKLIVSKDKRITV
                     KHVREMEQAGLQKMSVPPEFLTGRVLAHNVVDKESGEIIAQANDEITESLLNKLQEAQ
                     IGKISTIYINDLDQGPYISQTLRVDESADQLAAQVAIYRMMRPGEPPTEEAVKNLFHG
                     LFFSEERYDLSAVGRMKFNRRVGRNELGGTSTLSNRDIVDVIRILVELRNGRGEIDDI
                     DHLGNRRVRSVGELAENQFRAGLVRVERAVRERLSQAESENLMPHDLINAKPVSAAIR
                     EFFGSSQLSQFMDQTNPLSEITHKRRVSALGPGGLTRERAGFEVRDVHPTHYGRVCPI
                     ETPEGPNIGLINSLALYARTNQYGFLETPYRKVVNGKVTDEIHFLSAIEEGQYVIAQA
                     NAALDKKSKFVDELVSCRHHNEFAMSTPDRIQYMDVAPSQIVSVAASLIPFLEHDDAN
                     RALMGSNMQRQAVPCLRAEKPLVGTGIERTVAVDSGTAVQARRGGGVDYVDASRIVVR
                     VNDNETRAGEVGVDIYNLVKYTRSNQNTNINQRPVVKVGDAIARGDVVADGASTDIGE
                     LALGQNLLVAFMPWNGYNFEDSILISEKVVADDCFTSIHIEELSVVARDTKLGSEEIT
                     RDISNLSETMLSRLDESGIVYIGAEVEAGDVLVGKVTPKGETQLTPEEKLLRAIFGEK
                     ASDVKDTSLRVPSGMSGTVIDVQVFTREGIERDKRAQQIIDDELKRYKKDLADQLRIV
                     EDDAFGRIERLLIGKTANGGPKKLSKGTKLTKSYLAEVERHSWFDIRLANEQAARQLE
                     QLKESIAQKRKEFDAAYEEKKKKLTSGDELPPGVQTMVKVYLAVKRRLQPGDKVAGRH
                     GNKGVISKIVPVEDMPYMADGTPMDIVLNPLGVPSRMNVGQILETHLGWAAKGLGQKI
                     GEMLKAHAKVSDFRKALNLIYNSSGKSENITALSDEEVLNLAGNLKEGVPFSTPVFDG
                     ATEDEIKNMLELAGLPRSGQIALYNGHTGELFDRPVTVGYMHVMKLHHLVDDKMHARS
                     TGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASYTLQEMLTVKSDDVSGRTKVY
                     ENIVKGEHKIDAGMPESFNVLVKEIRSLAIDIDLDRY"
     gene            complement(6290..6670)
                     /gene="rplL"
                     /locus_tag="VLL03_03905"
     CDS             complement(6290..6670)
                     /gene="rplL"
                     /locus_tag="VLL03_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HSS46542.1"
                     /translation="MAVAKADILDAIANMTVLELAELIKQMETKFGVSAATAAVAVAA
                     PAVGGAAAVAEEKTEFTVILKTTGDNKVNVIKVVRAVTGLGLKEAKDLVDGAPKPVKE
                     GVSKADADNIVKQLTEVGATCEIK"
     gene            complement(6714..7238)
                     /gene="rplJ"
                     /locus_tag="VLL03_03910"
     CDS             complement(6714..7238)
                     /gene="rplJ"
                     /locus_tag="VLL03_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HSS46543.1"
                     /translation="MGLNLEEKKAVVAEVSAQLAKAQTVIIAEYRGTEVGDMTRLRAK
                     ARVAGVYFRVLKNTLARRAVAETPFAGLADNMKGPLAYGISTDPVVVARVLHEFAREN
                     ENFVIKGGAMANFVMSAKDIVSLAKMPSREELLAKLLGTLQAPIAQLVRTLNEVPGKF
                     VRTLAAIRDQRVKQ"
     gene            complement(7600..8286)
                     /gene="rplA"
                     /locus_tag="VLL03_03915"
     CDS             complement(7600..8286)
                     /gene="rplA"
                     /locus_tag="VLL03_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011901891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HSS46544.1"
                     /translation="MTSKRYKLITSKIDRSKLYALPDALKMVKQIATAKFDESVDVSV
                     NLGVDIKKSDQQVRGSVVLPAGTGKKVRIAVFAQGDKAKQAKDAGADIVGFEDLAEQI
                     KGGKIDFDVAIASPDAMRIVGQLGQILGPRGLMPNPKVGTVSADVAAAVKNAKAGQVQ
                     YRTDKAGIVQCTIGRASFTEDALRQNFLALIEALNKTRPSGIKGIYLKKISISSTMGV
                     GIRVDQASMT"
     gene            complement(8288..8719)
                     /gene="rplK"
                     /locus_tag="VLL03_03920"
     CDS             complement(8288..8719)
                     /gene="rplK"
                     /locus_tag="VLL03_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HSS46545.1"
                     /translation="MAKKIVGFIKLQVPAGKANPSPPIGPALGQRGLNIMEFCKAFNA
                     ATQKMEPGLPVPVVITAYQDKSFTFVMKTPPASVLILKAAKIEKGSPRPHTDKVGKIT
                     RARAEEIAKMKMPDLTAADLEAAVRTIAGSARSMGVEVEGV"
     gene            complement(8888..9421)
                     /gene="nusG"
                     /locus_tag="VLL03_03925"
     CDS             complement(8888..9421)
                     /gene="nusG"
                     /locus_tag="VLL03_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HSS46546.1"
                     /translation="MTKRWFVVHAYSGFEKSVQRALTDRIQRSDMQDKFGQILVPVEE
                     VVEMKSGQKSISERKFFPGYVLVEMEMTDETWHLVKSTPKVTGFVGGSANKPTPISEK
                     EVQNILQQIQEGVEKPKPKVLFEIGEAVRVREGPFTDFHGNVEDVNYDKSKLRVSVSI
                     FGRSTPVELDFGQVEKA"
     gene            complement(9421..>9653)
                     /gene="secE"
                     /locus_tag="VLL03_03930"
     CDS             complement(9421..>9653)
                     /gene="secE"
                     /locus_tag="VLL03_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HSS46547.1"
                     /translation="LGLAAVITWFTAPGKQFYAFSQEAVEETKKVVWPTRKETLQVTG
                     VVFLFVVVMALFLWGVDAVLLWAVRLLMGRSE"
BASE COUNT         1856 a         2976 c         2631 g         2190 t
ORIGIN      
        1 cacctgcatg tcgtccacgc aaatcgagat gcccgccttg gatgccagcg tgaagccgga
       61 atacatcagc ttgtcggcga aaatcaccgt ttcgcgcaag ccgcagcggc ggaagctcgc
      121 gttaatcagc ttggagattt ccttcttttt caacggctta ttaatgaggt cgaacggcag
      181 ccctttcggc agaatgtcgg aaaggagcgc ccgccctacc gtcgtgtcat ggcgggtgat
      241 tttttccttg aattcgctgc cggcgtcttt ctcgtattcc ttgatgcgca cgctgattcg
      301 ggcgttcagt tcaacctggc gcgaatcata agcgcgcgac acttcggcga catcagtaaa
      361 ctgcatgccc tcgccccagg cgttgatttt ctcgcgcgtg gtgtaataaa gacccagcac
      421 gatgtcctgt gacggcacga tgatcggctc gccgttggaa ggcgagagca cattgttggt
      481 ggaaagcatc agcgttcgca cctccatctg cgcctcgagt gacaaaggca catgcaccgc
      541 catctggtcg ccgtcaaagt cggcattgaa cgccgcacac accagcggat gcagctggat
      601 cgccttgccc tcgatcaaca ccggctcgaa agcttgaatc cctaagcgat gcaaagtcgg
      661 cgcgcggttc agcatcaccg ggtgctcgcg gatgacttct tcgagaatat cccagacttc
      721 cggcacttcg gcttccacca ggcgtttcgc cgccttgatg gtagcggaaa gccccagcac
      781 ttccagtttg tggaaaatga acggtttgaa cagctccagt gccattttct tcggcagacc
      841 gcattgatgc agcttcaatt gcgggccgac cacgatcact gagcggccgg agtaatccac
      901 gcgttttccc agcaggttct ggcggaagcg gccgcccttg cccttgatca tgtcggcgag
      961 cgatttcagc gcgcgcttgt tggcgccggt catggccttg ccgcggcggc cgttgtcgag
     1021 cagcgaatcc accgcttcct gcagcatgcg tttttcattg cgcacgatga tttccggcgc
     1081 cttaagctcc aacaggcgct tcaggcggtt gttgcggttg atgacgcggc ggtataaatc
     1141 gttcaaatca gaagtggcaa aacgcccgcc atcgagcggc accagggggc gcaactccgg
     1201 cggcagcacc ggcagcactt ccataatcat ccagtcgggc ttgatgcccg actggtggaa
     1261 cgcttccagc accttgaggc gcttggcgag cttcttaatc ttgctgtcgg agccggtcgc
     1321 ggccaattcc ttgcgcaggt tttcaatttc ctgattcata ttcaggttgc gcagcaatgc
     1381 gcggatgcct tccgccccca tgctcgcgga aaattcgtcg ccaaattctt cgaccttggc
     1441 gagataatca tcctcggaaa gcagctggca acgggtaagc ggtgtcatgc cggggtcggt
     1501 caccacatag gcttcaaaat acagcacgcg ctcgatgtcc ctgagcgtca tgtcaagcac
     1561 catgccgagg cgcgaaggca gcgacttcag gaaccagata tgcgcaaccg ggcttgccag
     1621 ctcgatgtga cccatgcgtt cgcggcgcac tttggacagg gtcacttcca cgccgcattt
     1681 ttcacaaatc acgccgcggt gtttaaggcg cttgtacttg ccgcacaaac attcgtagtc
     1741 cgtgaccggg ccgaaaatct tggcgcagaa caggccgtcg cgctccggtt tgaaggtgcg
     1801 gtaattgatg gtctccggtt ttttgacttc gccgtacgac caggatcgga ttttttccgg
     1861 cgacgcgagg ccgattttga tcgcgtcaaa ctcttcttcc tgagtgacct gcttgaacaa
     1921 atccagcaat gctttcatgt atgactcctg agtagggaga tcaataacta aacttcaaac
     1981 accggttaac cgcggaggcg cggggttcgc cgaaagattc tttcacgtca atgtttttct
     2041 ccgtatctct agcgtctctg cggtaaagac ttttccatca ataccggtcc agatcaatgt
     2101 cgatcgccag cgagcggatt tccttcacca gcacgttgaa cgactccggc atgccggcgt
     2161 cgatcttgtg ctcgcccttg acaatgtttt catagacctt ggtgcgcccg gacacatcgt
     2221 cggacttgac agtcagcatt tcctgcagcg tgtacgacgc gccgtaggct tcgagcgccc
     2281 acacttccat ttcgccgaag cgctggccgc cgaattgcgc cttgccgccc aaaggctgct
     2341 gggtgaccag actgtacgga ccggtggagc gcgcgtgcat tttgtcgtcc accagatgat
     2401 gtagcttcat cacgtgcata tagcccacgg tcaccggacg gtcgaagagc tcgccggtgt
     2461 ggccgttata aagcgcgatc tgtcccgagc gcggcaggcc cgccagctcc agcatgttct
     2521 tgatttcgtc ttcggtggcg ccgtcaaaca ccggagtcga aaacggcacg ccctccttga
     2581 gattgccggc aagattcagc acttcctcgt cggacagcgc cgtgatgttc tcgcttttac
     2641 cgctcgagtt gtaaatcagg ttaagtgctt tgcggaaatc ggaaaccttg gcgtgcgctt
     2701 tcagcatctc gccgattttc tgcccgaggc ctttcgccgc ccagcccaaa tgtgtttcga
     2761 gaatctggcc gacgttcatc cgcgagggca cgcccagggg attcaatacg atgtccatcg
     2821 gcgtgccgtc agccatgtac ggcatgtctt ccaccggcac gatcttggaa atcacgccct
     2881 tgttgccatg acggccagcc accttgtcgc cgggctgcag gcggcgcttg accgcgaggt
     2941 acacctttac catcgtctgc acaccgggcg gcagctcgtc gccgctggtg agtttcttct
     3001 tcttttcctc ataggcggca tcgaattcct tgcgtttctg cgcgatgctt tccttcagct
     3061 gctcaagctg gcgcgccgcc tgttcgttag cgagacgaat gtcgaaccag gaatggcgct
     3121 cgacttcggc aagataagac ttggtgagct tggtaccctt ggagagtttc ttcgggccgc
     3181 cattggcagt cttgccaatc agcagccgct cgatccggcc gaaggcgtcg tcttcgacaa
     3241 tgcgcaactg atcggccaaa tccttcttgt agcgcttgag ctcatcgtcg atgatttgct
     3301 gggctcgctt gtcgcgttcg atgccttcgc gggtgaacac ctgcacgtcg atcaccgttc
     3361 ccgacatgcc ggacggcacg cgcaggctgg tgtctttaac gtcggatgcc ttctcgccaa
     3421 aaatcgcgcg cagcagtttt tcctcgggcg tgagctgcgt ttcgcctttc ggggtcactt
     3481 tgcccaccag cacatcgcct gcctcgactt cggcgccgat gtagacgatg cccgattcat
     3541 cgaggcgcga cagcatcgtc tccgaaagat tggaaatatc gcgtgtgatt tcttccgacc
     3601 ccagcttggt atcgcgtgcc acgaccgaca attcctcgat gtgaatcgaa gtgaagcagt
     3661 cgtccgccac tactttctcg gaaatcagga tcgagtcctc aaagttgtag ccgttccacg
     3721 gcatgaaggc gaccagcagg ttttgcccca gcgccagctc gccgatgtcg gtggatgcgc
     3781 cgtcggcaac cacatcgccg cgggcaatcg catcccccac cttgaccacg gggcgttgat
     3841 tgatattggt attttggttg gagcgggtgt atttcaccaa attataaata tccactccca
     3901 cctcaccggc gcgggtttcg ttgtcgttca cccgcaccac gatgcgcgag gcgtccacat
     3961 aatccacgcc gccgccgcgg cgcgcctgta ccgcggtgcc ggagtccacc gccacggtgc
     4021 gctcgatgcc ggtaccaacc aaaggcttct ctgcgcgcag acacggcacc gcctggcgct
     4081 gcatgttgga acccatcaag gcgcgattcg cgtcgtcgtg ctcgaggaac ggtatcaatg
     4141 acgccgccac cgacacaatt tgcgacggcg ccacgtccat gtattgaatg cggtcgggcg
     4201 tggacatggc gaactcgttg tgatggcggc aggacaccag ttcgtccacg aacttgctct
     4261 ttttgtcgag cgcggcgttt gcctgcgcga tcacgtactg cccttcctcg atcgctgaca
     4321 gaaaatgaat ctcgtcggtc accttgccgt tgaccacctt gcggtacggc gtctcgagga
     4381 agccgtactg gttagtgcgg gcgtacagcg ccagcgaatt gataagaccg atgttcgggc
     4441 cttccggcgt ttcaatcggg cacacgcggc cgtagtgcgt cggatgcacg tcacgcactt
     4501 cgaagccggc acgctcgcgc gtgaggccgc ccggccccag ggcggacacg cgccgcttgt
     4561 gggtgatttc ggaaagcggg ttggtctggt ccatgaactg cgagagctgg gaagaaccaa
     4621 agaactcgcg gatggccgcg gacaccggct tggcattgat caagtcgtgc ggcatcaggt
     4681 tttccgattc cgcctgcgac agccgctcgc gcaccgcgcg ttcgacgcgc accagtcccg
     4741 cgcggaactg gttttccgca agttcgccca ccgagcgcac gcggcggttg cccaggtgat
     4801 caatgtcatc gatttcgccg cgtccgttgc gcagctccac cagtatcctg atgacatcca
     4861 cgatgtcccg gttggaaagc gtgctcgtgc cgccgagctc gttgcgcccg acgcggcggt
     4921 tgaatttcat gcgccccacg gccgaaaggt catagcgctc ctcggagaag aacaggccgt
     4981 ggaacaagtt tttcaccgct tcttcggtcg gcggttcgcc ggggcgcatc atgcggtaaa
     5041 tcgccacctg cgccgccagt tggtcggcgc tttcgtccac gcgcaaggtc tgcgaaatat
     5101 aggggccttg atcgagatca ttgatgtaaa tggtggagat tttgccgatc tgcgcttcct
     5161 gcaatttgtt gagcaggctt tcggtgattt catcgttggc ttgcgcgata atctcgccgc
     5221 tttccttgtc caccacgttg tgcgccagca cgcgcccggt caggaactcc ggcggcacgc
     5281 tcattttctg caagccggcc tgctccattt cgcgcacatg cttgaccgtg atgcgcttgt
     5341 ccttggacac gatcagcttg ccgcccctgg tagtgatgtc gaagcgcgcc acttcgccgc
     5401 gcatgcgctc gggcaccagt tcgaattgaa ttcccttctt gctgatgtgg aaagtgtcga
     5461 acgcgaagaa ttcggccaga atctgctccg gtgtcatgcc gatggctttc aatagcgtcg
     5521 tcaccggcat cttgcgccgg cggtcaacac ggaaatacag gtaatccttg ggatcgaact
     5581 cgaaatccag ccacgagccg cggtagggaa tcacgcgcgc ggagaacagc agtttgccgg
     5641 aggagtgggt cttgccgcga tcgtgctcga agaacacgcc gggcgaacgg tgcagctgcg
     5701 atacgatgac gcgttcggtg ccgttgatca cgaacgagcc ggttaaggtc atgagcggaa
     5761 tctcgcccat gtacacttcc tgctccttca cttccttcac cgtcggcttg ctggcctcac
     5821 ggtccatgat ggtcagccgc accttggcgc gcagcggcgc ggcgaaggtg agcccccgct
     5881 gctggcattc cttgacgtcg aacggcggcg tgcccagcgc gtagctcacg aaatccagcc
     5941 gggcgttctt ggagtggctc tcgatcgggt aaatcgaagt aaacgcggcc tgcagccctt
     6001 cgttcttgcg cttttccggt ggaacctcgg cctgcaggaa ggaagcgtac gaatccagct
     6061 gcgtggcgag aagaaacggc accggtagca cgctggcgcg cttggcaaaa ctcttgcgga
     6121 tgcgtttttt ctcggtaaat gagtagctca tatcaactcc agatagttaa acgcccgggc
     6181 aaagtgtaaa cgaaactttc acgttgccaa atgcaaaaat gcgcttgaac aaaagccaac
     6241 aggctggcgg tttcccgcca gcccgtttcc cgcaccatga agtacacgct tacttgattt
     6301 cgcaagtcgc gccgacttcg gtcagttgct tgacgatatt gtcggcgtcg gctttggaca
     6361 cgccttcctt caccggcttt ggcgcaccgt ccactaggtc cttggcttct ttcagaccca
     6421 ggccggtcac cgcgcgcact actttaatca cgttgacctt gttgtcaccg gtagttttga
     6481 gaatcacggt gaattccgtt ttctcttcgg ccacggcggc ggcaccgccc acggccggcg
     6541 ccgcgaccgc cactgcggca gtcgcagcag acacgccgaa cttcgtttcc atctgcttga
     6601 tgagctcggc gagctccagc accgtcatat ttgcaattgc atccagaatg tcggctttag
     6661 caacagccat gattttctcc tagtaaaaaa tgttgattca gtcgcgtagt ccgtcactgc
     6721 ttaacgcgct ggtcacgaat ggcggccagc gtgcgcacaa atttacccgg tacttcgttt
     6781 agagtccgca ccaattgcgc gatcggcgcc tgcagcgtgc ccagaagctt cgccaacagt
     6841 tcctcgcggc tcggcatctt ggccaggctg acgatgtcct tcgcgctcat cacgaagttg
     6901 gccatcgcac cgcccttgat gacgaagttc tcgttttccc tggcaaattc atgcagcact
     6961 ctcgccacca ccaccggatc ggtcgaaatg ccataggcca gcggaccctt catgttgtcc
     7021 gccaagccgg caaacggcgt ctccgccacg gcacggcgcg ccaaagtgtt tttaagcacg
     7081 cggaaataca cgcccgcgac gcgcgctttc gcgcgcagac gggtcatatc accgacttcc
     7141 gtgccgcgat actcggcgat gatgacggtc tgagccttgg caagctgggc gcttacctcg
     7201 gccacgactg cctttttctc ttcaagattt agacccaagg cctatccctc cttgacttgt
     7261 aaccggctgc gtcgccgcaa ccgaaactca cttaagcgac cttcgatcag gtaatacaaa
     7321 tcctgctgtc gggtgcgcca tctgcgttgg gcgatgaacg tgaaacgtga agagtaaaaa
     7381 gtgaaacgta agcttcctct cccacgcccg ttcgaacaat taagtgtctg gatttcccag
     7441 tgccccattc cttgcaaaac gtaatcaaca cgtttcacct ttcacgtttc acggttcacc
     7501 catccacccc aacggtcttt gacagcacgg ctaattacct agatacatca gccggcccca
     7561 aagtttgcat gcgggcgcaa cgcccgcgaa aaattttcct tatgtcatgc tcgcctgatc
     7621 cacccggatt cccaccccca tggtgctgga aatggaaatc ttcttgaggt agatgccctt
     7681 gatgcccgaa ggccgggtct tgttcagcgc ttcaatcagc gccagaaaat tctggcgcag
     7741 cgcatcctca gtaaacgagg cgcggccgat cgtgcattgc acgatgccgg ccttgtcggt
     7801 gcggtattgc acttgtcccg ccttggcgtt tttcaccgcc gcagcgacgt cggcggacac
     7861 cgtccccacc ttggggttgg gcatcagtcc acgcgggccg agaatctggc ccaactgacc
     7921 gacgatacgc atcgcatcgg gcgaggcgat cgccacatca aaatcaatct tgccgccctt
     7981 gatttgctcg gcaagatctt caaaaccgac aatgtcggca ccggcgtcct tcgcctgctt
     8041 ggccttgtcg ccctgcgcaa acaccgcgat gcgcactttc ttgccggtgc cggcaggcag
     8101 caccaccgaa ccgcgcacct gctggtcgga tttcttgata tccacgccga gattgacgga
     8161 cacgtcaacc gactcgtcga atttggcggt ggcgatttgt ttgaccatct tcagcgcgtc
     8221 aggcagcgca taaagcttgc tgcgatcaat cttggaagta atcagcttgt aacgtttgga
     8281 ggtcatgtca caccccctcg acttccacgc ccatgctgcg cgcgctgcca gcgatggtgc
     8341 gcacagcggc ctccaagtcg gccgcggtca aatcaggcat tttcatcttg gcgatttctt
     8401 cagcccgggc gcgcgtgatc ttgcctacct tgtcggtgtg cggacgcggg ctgccttttt
     8461 caattttcgc cgctttcagg ataagcaccg acgcgggcgg cgtcttcatc acgaaggtga
     8521 agctcttgtc ctggtaggcg gtgatcacca ccggcaccgg caggccaggt tccattttct
     8581 gcgtggcggc gttgaacgcc ttgcagaatt ccatgatgtt gagtcctcgc tggccgagcg
     8641 cgggaccgat gggcgggctg ggattggcct tgcccgccgg aacttgcagc ttgatgaagc
     8701 cgacgatttt ctttgccacc ttactctcct aagcgggtgc ggttggtttg gcgttgagtg
     8761 ttcagagttg agcgtaaacc ctgaaacgtc tatcgccttt ccaacctccc cgttggttac
     8821 atacatgggt acattattca atgcaccgct tcaatcacgc tcaacgttaa cgccgaacgc
     8881 tcaaccttta cgccttttca acctgtccga aatccagctc gaccggcgtg gagcggccga
     8941 aaatcgagac ggacacgcgc aacttgcttt tgtcataatt cacatcttcg acgttgccgt
     9001 ggaaatcggt gaacggtcct tccctcacgc gcacggcttc gccgatttcg aacaacacct
     9061 tgggcttggg tttctccaca ccctcctgga tctgctgcag gatgttctgc acttcctttt
     9121 cgctgatggg cgtgggcttg ttggcgctgc caccgacaaa gccggtaact ttcggcgtac
     9181 ttttcaccag atgccaggtt tcgtcggtca tttccatctc caccagcacg tagccgggga
     9241 aaaacttgcg ctcggaaatg cttttctggc cgcttttcat ctccaccact tcttccaccg
     9301 gcaccagaat ctgcccgaac ttgtcctgca tgtcggaacg ctggatacga tcggtaagcg
     9361 cacgctgcac gcttttctca aagcccgaat aggcatgaac cacgaaccac cgtttcgtca
     9421 ttattcgctt cgccccatca gcaatctcac cgcccacagc aagacggcat ccacccccca
     9481 caggaacaat gccatcacta ccacgaacag gaaaaccacg ccggtcacct gcaaagtttc
     9541 cttgcgcgtc ggccacacca cttttttggt ttcttcgacc gcttcctgac tgaaggcata
     9601 aaactgcttg ccgggagcgg taaaccaggt aatcaccgcc gccagcccca gcc
//
LOCUS       DATIOW010000119         1726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1884492, whole genome shotgun sequence.
ACCESSION   DATIOW010000119 DATIOW010000000
VERSION     DATIOW010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884492"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VLL03_03935"
     CDS             complement(<1..248)
                     /locus_tag="VLL03_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HSS46548.1"
                     /translation="MQHIARKRFGQNFLVDREIIDAIIAAIAPQSSDRMVEIGPGMGA
                     LTRPLLERLKHLHVVEIDRDIVARLISEFPASRLTVHAA"
     gene            complement(288..1334)
                     /gene="pdxA"
                     /locus_tag="VLL03_03940"
     CDS             complement(288..1334)
                     /gene="pdxA"
                     /locus_tag="VLL03_03940"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HSS46549.1"
                     /translation="MPRANSLPTLAVTAGEPAGIGPDLCVLLARHEFPARIVVIADKT
                     LLAERAVMLKKKIKLVDFTPGRVRALAPGSLLVLQEPLAKSVVPGKPDPANSGYVLRT
                     LQQAVRGCLEKHFDAMVTAPVHKGVINDAGIPFTGHTEYLAQQTATRHVVMLLTGGGL
                     RVALATTHLALKDVPSALSRDGLERCLRILHHDLVTRFGITAPRILVAGLNPNAGESG
                     YLGNEETQIIGRVVKKLLCKGFDVKGPLPADTLFTHERLKSCDCALAMYHDQGLPVLK
                     YASFGSGVNVTLGLPIIRTSVDHGTALELAGTGKIDCGSLLAAIKLAARLSNTAKRQA
                     TKRVNHTLPAGIER"
     gene            complement(1402..>1726)
                     /locus_tag="VLL03_03945"
     CDS             complement(1402..>1726)
                     /locus_tag="VLL03_03945"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSS46550.1"
                     /translation="ELARLQSEDGSAAQGGDLGWLVPGDTVPEFERAMNALKIGQFSD
                     PVRSPFGWHLIQVLERRNQDISRDRQRLLARQALRARKADEAHEEWLRQLRDSAYVEY
                     RLEDK"
BASE COUNT          350 a          495 c          508 g          373 t
ORIGIN      
        1 gcggcgtgta ccgtaagccg cgacgcggga aattcgctaa tcaagcgcgc cacgatgtca
       61 cggtcaattt ccacgacgtg aaggtgttta agccgttcca gcaacgggcg ggtgagcgct
      121 cccatgccgg gcccgatttc caccatgcga tccgagcttt ggggggcaat cgctgcgata
      181 atggcgtcta tgatttcccg gtcaaccaga aaattctggc caaaacgttt tctggctatg
      241 tgctgcatcg ttgcgggatt ggacgagggt tggcggcggt tgcccgttta tctttcaata
      301 ccggctggca gggtatgatt tacgcgtttt gtcgcctggc gcttggcggt attggaaagc
      361 cgcgctgcga gtttgattgc cgccaacagg cttccacagt cgattttacc ggtccctgcc
      421 agttcgagcg cagtaccgtg gtcgactgaa gtgcggataa tgggcaaacc cagcgtgaca
      481 tttacccccg agccgaaact tgcgtatttc agcacgggaa ggccctgatc gtggtacatc
      541 gccagagcgc agtcgcagct tttaaggcgt tcatgagtga acaaggtgtc ggcgggaagc
      601 ggccccttca catcaaaacc cttgcaaaga agcttcttca ccacccggcc gataatttgc
      661 gtttcctcgt tcccgagata gccggattcg ccggcgttgg gattcaggcc cgccaccaga
      721 atgcgcggcg cggtaatgcc aaatcgcgtc accagatcat gatgcaggat gcgtaagcaa
      781 cgttccaagc cgtcccggct gagcgcagac ggcacgtctt tcaaagcgag atgggtagtg
      841 gcaagcgcga cccgtaaacc ccctccggtg agcagcatta ctacatgccg ggttgccgtt
      901 tgctgcgcca gatattcggt atgcccggta aaagggatgc cggcatcgtt gattacgcct
      961 ttgtgaaccg gcgcggtcac catggcatcg aaatgctttt ccaggcagcc tcgcactgct
     1021 tgttgcaggg tgcgcagcac atagccgctg ttggcgggat ccggttttcc tggaaccacg
     1081 gacttggcaa gcggctcttg cagcaccaac aagctgcctg gagcgagtgc tctaacgcga
     1141 cccggggtga aatcaaccag cttgattttt ttctttagca tcacagcgcg ctcggcaagc
     1201 agggttttgt ccgcaatcac tacgatgcgc gccgggaact cgtgccgcgc caacagcaca
     1261 cacaaatccg gcccgatgcc cgcaggttcc ccggcggtca cggcgagggt tggtagtgaa
     1321 ttagcgcgtg gcatgagtaa aattaagcgc ggaaacgaac gcagagatta atgtgaattt
     1381 ctctgcgctg ctgaggtgaa tttatttatc ttccaagcgg tattccacgt acgcgctgtc
     1441 gcgcaactgg cgcagccatt cttcgtgggc ttcatcggcc ttgcgcgcgc gcaaggcctg
     1501 tcgcgcaagc aggcgttgac ggtcacggct gatatcctga ttgcggcgtt ccaacacctg
     1561 gatcaggtgc cagccgaaag gcgatcgcac cggatcgctg aattgcccga tcttgagtgc
     1621 attcatcgcg cgttcgaact cgggcaccgt atcgcctgga acaagccaac ccaagtcgcc
     1681 gccctgcgcg gcactgccgt cttcggattg cagccgagcg agttca
//
LOCUS       DATIOW010000120         3211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1276115, whole genome shotgun sequence.
ACCESSION   DATIOW010000120 DATIOW010000000
VERSION     DATIOW010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276115"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /gene="cmk"
                     /locus_tag="VLL03_03950"
     CDS             complement(<1..630)
                     /gene="cmk"
                     /locus_tag="VLL03_03950"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HSS46551.1"
                     /translation="MKELGNIPVIAIDGPSASGKGVVAQWVARELGFHYLDSGSLYRL
                     VALAAQKRGMAPGDETALAKLAESLPAEFRVGEILLDGKQVTDEIRSETCAASASRVA
                     ALPQVRLALLSRQRAFRKTPGLVAEGRDMGSTVFPDAVLKVFLTASTEARAERRYKQL
                     MEKGMYASISTLLQEIKERDLRDSQRSVAPLRQGADAGLLDTTSLSIEET"
     gene            complement(703..2025)
                     /gene="aroA"
                     /locus_tag="VLL03_03955"
     CDS             complement(703..2025)
                     /gene="aroA"
                     /locus_tag="VLL03_03955"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HSS46552.1"
                     /translation="MTVNYRIEPGGALRGKLRVPGDKSISHRSIMLGSIAEGINKIGG
                     FLEGEDVLATMNALRAMGVRIEGPDQRSVVVHGVGLRGLKAPPKVLDCGNSGTSMRLL
                     CGLLAGQSFNCELDGDSSLRQRPMQRVVAPLRGMGADIVTAGNGRPPVKIRGMPELRG
                     IEYVMPVASAQVKSALLLAGLYARGRTCVTEPAPTRDHTERMLQAFGYPVRREGGKIC
                     VNGGGKLQATAIEVPADISSAAFFMVGAGIAPVSEITLSHVGINPTRTGVIEILRLMG
                     ADITLANERTAGGEPVADIRVRHARLKGVNIPAELVPLAIDEFPVLFIAAANAEGETV
                     LTGAEELRVKESDRIQVMADGLHALGIKAQPAPDGIRILGGTYHGGEIDSHGDHRIAM
                     SFAIAALRASSPITIRDCANVATSFPGFVQLALTAGLKISENLNCGGR"
     gene            complement(2031..2903)
                     /locus_tag="VLL03_03960"
     CDS             complement(2031..2903)
                     /locus_tag="VLL03_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HSS46553.1"
                     /translation="MAKPLLNKLVIFGVGLIGGSFALALKRAQAVKHIVGVGRSPENL
                     KKALKLRVIDEITDSPARAVRGADLILLAMPVGQMRQVMRAIAPHLEPHTIVSDAGST
                     KQDVVALARECLASHLQRFVPAHPIAGAELSGVAAAHADLFVQKNLVLTPLANTDKKA
                     LQRVKDLWLVCGAKVSQMTPRRHDEVFAAVSHLPHLLAYALVDAIANKANGAELFKYA
                     AGGFRDFTRIAGSSPEMWRDVALSNRRILLEELADYQRQLKRLAGLLQKNNGKALEKI
                     FANARDARRKWLPS"
     gene            complement(2905..>3211)
                     /locus_tag="VLL03_03965"
     CDS             complement(2905..>3211)
                     /locus_tag="VLL03_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSS46554.1"
                     /translation="LAALDDHEFVKQSFELNQKGMAQLMQAFKRLGLRYIPSYGNFVC
                     FHADDAAALYQRLLRRGVIVRPIANNGMPEWLRVSIGLPHENEKFLKALESLMREAA"
BASE COUNT          655 a          988 c          872 g          696 t
ORIGIN      
        1 cgtttcctcg atgcttaacg aggtggtatc caaaagacca gcatcggccc cctgtcgcag
       61 gggcgccacg gaacgctgac tgtcacgcag atcacgttcc ttaatttcct gtaaaagggt
      121 tgaaatgcta gcatacattc ctttttccat caactgttta tatctgcgct cggcgcgcgc
      181 ttcggtgctg gcggtcagaa agacttttaa gacggcatcg ggaaacacgg tggaacccat
      241 gtcgcgcccc tccgccacca gacccggagt tttgcggaaa gcgcgctgtc tggagagaag
      301 cgcgagccgg acttggggca gcgcagcgac ccgggatgcg ctcgcagcgc aagtttccga
      361 gcggatttca tccgtcactt gcttcccgtc cagcagaatt tcaccgacgc gaaactccgc
      421 gggcaggctt tccgccaatt tagccaatgc tgtttcatca ccgggcgcca ttccacgttt
      481 ttgcgcggcc aaggcgacca agcggtagag cgagccgctg tccagataat ggaaaccaag
      541 ttcccgcgcc acccattgcg ccactacgcc cttgcccgag gccgaaggcc cgtcaatggc
      601 gatgactggg atattgccaa gttctttcat aaaaatcttt tcaccgcagg agacgccgag
      661 gcaaggaatg aaaaactatt ttcttggctt tcctctgcgt tctcagcgtc ctccgcagtt
      721 aaggttttcc gaaattttta atcccgccgt cagtgcaagt tgcacaaaac caggaaacga
      781 cgtcgccaca ttggcacaat cgcgaatggt aatcgggctg gaagcgcgca aggcggcaat
      841 ggcaaacgac atggcgatgc ggtggtctcc gtgactgtcg atttcaccac cgtgataagt
      901 cccgccgaga atgcggatgc cgtccggcgc gggttgtgct ttgattccga gcgcatgcaa
      961 gccatcggcc atcacctgga tgcggtcgct ttccttgacg cgcagttctt ccgcgccggt
     1021 caatacagtc tccccttcgg cattcgccgc cgctatgaac aaaaccggaa actcgtctat
     1081 cgccagcggc acgagttcgg cgggaatatt cactccttta agccgagcat ggcgcacccg
     1141 aatatccgcc accggctccc cgcctgccgt gcgttcattg gcaagcgtga tgtcggcgcc
     1201 cattaaacgc aaaatttcga tcactccggt gcgggtggga ttgatgccga cgtggctgag
     1261 cgtgatctcc gaaacgggcg cgatgccggc gcctaccatg aaaaacgccg ctgaggaaat
     1321 gtcggccggc acttcgatgg cggttgcctg taatttcccg ccgccgttca cgcaaatttt
     1381 cccgccttcg cgtctaacgg gatagccaaa cgcctgcaac atgcgctcgg tgtggtcgcg
     1441 ggtcggcgcc ggttcggtga cgcaggtgcg ccctctggca taaagccctg ccagcagcag
     1501 tgccgatttt acctgggcgc ttgctaccgg catgacgtat tcaattccgc gtaattccgg
     1561 catgccgcgg atttttacgg gcggcctacc gttgccggcg gtgacaatat ccgcccccat
     1621 tccccgcaaa ggagccacca cacgttgcat cggccgttgg cgcaggctgg aatcgccgtc
     1681 caattcgcaa ttaaaagact ggccggcgag cagtccgcat aaaagccgca tcgaggtgcc
     1741 tgaattgccg caatccagaa ctttcggcgg cgctttcagg ccgcgcaatc ctaccccgtg
     1801 caccaccaca cttctttgat ccggcccttc gatgcgcacg cccatggcac gcaaggcatt
     1861 catggtggca agcacatcct cgccttcgag gaatccgcca attttgttta taccctcggc
     1921 aatggagccc agcatgatgc tgcgatggga aatggatttg tcgccgggaa cgcgcagctt
     1981 gcctcgcagc gcaccacccg gctcgatacg gtaattgacg gtcattgtta ctaactcggc
     2041 agccatttgc gccgggcgtc gcgggcattg gcaaagattt tttcaagcgc tttgccatta
     2101 ttcttctgca acaatccggc caggcgcttc aattgccgct gatagtcggc aagctcttcc
     2161 agtagaattc tgcgattgga aagcgcgacg tcgcgccaca tttctgggga actccccgct
     2221 atccgcgtga agtcgcggaa gccgcccgcg gcatatttga aaagctcggc gccgttggct
     2281 ttgttcgcaa ttgcatccac cagcgcgtag gcaagcaggt gcggcaaatg gctcaccgca
     2341 gcgaaaactt cgtcgtgccg gcgcggcgtc atttggctta cttttgcgcc gcagacgagc
     2401 cacaaatcct tgacccgctg caatgccttt ttgtcggtat tggccaaagg tgtcagcacc
     2461 aggttttttt gcacaaaaag atcagcatgc gccgcagcca cgccgctcag ttccgcgccg
     2521 gctattggat gcgcgggaac aaagcgctgc aagtgtgaag caaggcattc cctggccaat
     2581 gccactacat cctgcttggt actgccggca tcgctgacaa ttgtatgcgg ctccaggtgt
     2641 ggcgcgatgg cgcgcatgac ttggcgcatc tgccccaccg gcatcgccag caagatcagg
     2701 tcggcgcccc gcaccgctcg tgccggacta tcggtgattt catcaatcac gcgcagcttc
     2761 agtgcctttt tcaggttttc cgggctgcgt ccgacgccga cgatatgctt caccgcctgc
     2821 gcacgcttca acgccagggc aaacgagccg ccgatgagac cgacgccaaa aatcaccagc
     2881 ttgtttaaca ggggtttcgc catttcatgc cgcttcgcgc atcaggcttt ccaaagcttt
     2941 aaggaatttt tcgttttcat gcggtaagcc tatgcttact cgcagccatt ccggcatgcc
     3001 gttattggcg atcggccgca cgattacgcc gcgcctgagc aggcgctgat acagtgccgc
     3061 cgcatcgtcc gcgtggaagc acacaaaatt tccatacgaa ggaatataac gcagtcccag
     3121 acgcttgaat gcctgcatga gctgcgccat gcctttttga ttaagctcga agctttgttt
     3181 cacgaactca tggtcatcga gcgccgccag c
//
LOCUS       DATIOW010000121         3257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1277113, whole genome shotgun sequence.
ACCESSION   DATIOW010000121 DATIOW010000000
VERSION     DATIOW010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277113"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /gene="mutM"
                     /locus_tag="VLL03_03970"
     CDS             complement(<1..645)
                     /gene="mutM"
                     /locus_tag="VLL03_03970"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HSS46555.1"
                     /translation="MPELPEVEITRRGLEPRLSGRRIAKVAIRNPDLRWQVAADLPRL
                     VSGQVIQNISRRGKYLLIGCGSGTLIVHLGMSGSLRFLDEAVPAQKHDHFDLLLDNGE
                     MLRLRDPRRFGAVLWTSADPLTHPLLKELGPEPLTRAFNADWLYRQMRGRSVAIKQAL
                     MDSHVVVGVGNIYSNEALFRAHINPKTSACRIGPERCRKLVDAVRETLRLAIKAG"
     gene            complement(659..1498)
                     /locus_tag="VLL03_03975"
     CDS             complement(659..1498)
                     /locus_tag="VLL03_03975"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HSS46556.1"
                     /translation="MKSRIVLNLVLFTLVVGIGLLLYFKPQSNTTEYRLSALQAGDIK
                     RITIDKPAALSLEMHDGGWFIINTFKARADAVQVERLLDILSASSKQRFPANDLARFD
                     LDQPQARLTLGEQTFSFGALNTLTREQYVATADNVYLVAPRYGAAAAMPLNNLLNHKL
                     LADSEIPTAFDLPGYKLTRVNGAWSVSPTPAQLSQDQLNRFADEWRHSYSIASEPAKP
                     VPGAEQIKITLNKGGSLLLQILQKQPQLILLRADEMIAYRFPEDVARRLLDPFAAASS
                     EEK"
     gene            complement(1639..2991)
                     /locus_tag="VLL03_03980"
     CDS             complement(1639..2991)
                     /locus_tag="VLL03_03980"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HSS46557.1"
                     /translation="MEINKRLRIQLLLQNSLFVLLLITAVVLLSLLARDYTRQWDISQ
                     NGRNSLTQASLDVLKQLPGPLTITAFATPQDARLGDVRKLIQDFMLPYQRAKPDIRLE
                     FVDPAEQPKLASEAGVQVNGEMVVKFGAKSEHLTALNEQTFTNLLMRLARAQQRLVMQ
                     LEGHGERKLDGKANHDLGEFGKQLAIKGFQTASVNLTLAQEVPDNASMLVITQPQVDW
                     LPGEIAKLKRYLDKGGNLLWLVDPEPLHGLQALADTLRIELTAGVVIDPAAQQLNAPA
                     TWALGASYGRHPIIRDFNLITVFPFAREVRVNQNRDWQSTTLIEVAQRGWVETGKLDG
                     EVRFDKNRDTPGPVPIGVALVKKLKGREQRVVVIGSGAFLANSYLGNAGNLDLGVNII
                     NWLAQDESLITIQPRATLDSNLNLGKTALTLISVGFLILLPLIFLVTGGVIWWRRRKL
                     "
BASE COUNT          677 a          935 c          949 g          696 t
ORIGIN      
        1 gcccgcctta attgccaggc gcagcgtttc acgcactgca tccaccagtt tgcggcagcg
       61 ctcaggaccg atgcggcagg ccgaggtctt gggattgata tgggcgcgga acagcgcttc
      121 gttggagtaa atattcccga cccccacgac tacatggctg tccatcagcg cttgcttgat
      181 ggccacgctg cggccgcgca tttgccggta cagccagtcc gcgttgaaag cgcgggttaa
      241 cggctcgggg ccgagttcct tgagcagcgg atgggtgagc ggatcggcgc tcgtccacag
      301 caccgcgccg aaacggcgcg gatcccgcaa gcgcagcatt tcgccgttat ccagcagcaa
      361 atcaaaatgg tcatgctttt gcgctggaac cgcctcatcc aaaaagcgca agctgcccga
      421 catgccgaga tgcacaatca atgtgccgct gccgcagccg ataagcaggt atttgccgcg
      481 ccgcgagata ttttggatga cctggccgct aaccaagcgc ggcaaatccg ctgccacttg
      541 ccagcgcaag tcgggattgc gaatggcgac cttggcgatg cgcctcccgc ttaagcgcgg
      601 ctcgagaccg cgccgcgtga tttctacttc cggaagttct ggcatggtat aggcgcagtc
      661 atttttcctc gctggatgca gctgcgaacg ggtcgagcag gcgtcttgcc acatcctcgg
      721 gaaaacgata agcaatcatt tcatcagcgc gcagcaatat taattgcggt tgtttttgca
      781 atatctgcag caacaagctg ccgcccttgt taagcgtaat cttgatttgc tccgcacccg
      841 gcaccggctt ggcgggctcg ctggcgatac tgtaagagtg gcgccattca tcggcgaagc
      901 ggttcaattg atcttgggag agttgagccg gagtggggga cacgctccaa gcgccattca
      961 cccgcgtgag cttgtatccc ggcaaatcga aagcagtcgg tatttcggaa tcggccagca
     1021 gcttgtggtt gagcagattg ttgagcggca tggcggcggc tgcgccgtag cgcggtgcca
     1081 ccaaatagac gttatccgct gtagccacat attgttcccg ggtcagcgta ttgagcgcgc
     1141 cgaaactgaa ggtctgctcc cccagcgtca agcgtgcctg cggttggtcc agatcaaagc
     1201 gcgctaaatc attcgccggg aaacgctgct tgctgctggc ggagagaatg tccagcaggc
     1261 gttccacctg cacggcgtcg gcgcgcgcct tgaacgtgtt gatgatgaac cagccgccat
     1321 cgtgcatttc caggctgagt gccgcgggtt tgtcaatcgt gatgcgcttg atatcacctg
     1381 cttgcagcgc ggaaagccgg tattcggtgg tattgctttg cggtttgaag tacaacagca
     1441 gacctatgcc cacaaccagg gtgaacaaaa ccagatttag aacaatgcgt gatttcatgc
     1501 cggaaactct tggcaaattg atgcataagc tgttctggcg cggcacaaaa ttgcgagcaa
     1561 agcggagttt acacggcagt aaatgagcat ttgcgagcaa ttttcaacaa agccagggca
     1621 gcttaggcac aatttgcttc atagtttgcg ccgccgccac caaataacgc caccagtcac
     1681 cagaaaaatc agcggcaaca ggatgagaaa gcccacgctg atcaacgtca gtgcggtttt
     1741 accgagattg agattgctgt ccagagtggc gcgcggctga atcgtaatca agctctcatc
     1801 ctgcgccagc cagttgatga tgttcacacc cagatcgagg ttgccggcgt tgccaagata
     1861 gctgttggca agaaaagcgc cgctgccgat taccactacc cgctgttcgc gtcctttgag
     1921 ttttttcacc agggcaacgc ctatcggcac cgggccggga gtatcgcggt ttttgtcgaa
     1981 gcgcacctct ccgtccagtt tgccggtttc cacccagcca cgctgcgcta cttctatgag
     2041 cgtggtggat tgccaatccc ggttttgatt aacgcgcacc tcgcgggcga agggaaacac
     2101 cgtgatcagg ttgaagtccc ggataatcgg gtgcctgcca tagcttgcgc ccaacgccca
     2161 agtggcaggc gcgttaagct gttgcgccgc gggatctatc actacgccgg cggtgagttc
     2221 aatccgcagc gtatcagcga gcgcttgcag gccgtgcaac ggctccgggt caaccagcca
     2281 cagcagatta ccgcccttgt cgagatacct tttcagcttg gcgatttcgc cgggcagcca
     2341 atccacttgc ggctgggtaa tgaccagcat gctggcattg tcgggaactt cttgcgccag
     2401 cgtcagattc acgctggcgg tctggaaccc tttgatggcg agttgtttgc cgaattcgcc
     2461 aaggtcgtga ttggctttgc cgtccagctt cctttcgcca tgcccttcga gctgcatcac
     2521 cagccgctgc tgcgctcgcg ccaggcgcat cagcaaattg gtgaatgttt gttcattgag
     2581 cgcggtcaga tgttcggatt tggcgccgaa cttgaccacc atttcgccat tgacttgcac
     2641 gccggcttcg ctggcaagct tcggctgttc cgcgggatcc acaaattcca gccggatatc
     2701 gggtttggcg cgctgatagg gcagcataaa atcctggatc agtttgcgca catcgccgag
     2761 ccgcgcatcc tgcggtgtgg cgaaggcagt gatagtcaga ggtccgggca gttgcttgag
     2821 cacgtcaagg ctggcctggg tcaacgagtt gcggccgttc tggctgatgt cccactgcct
     2881 agtgtaatcg cgcgccagca acgacagcag aacaaccgct gtgatcagca gcaacacgaa
     2941 cagactgttt tgcagcagta actgaattcg aagtctttta ttaatttcca tagctagtta
     3001 aggaacctct gattgagtcc ctcgcaattg cgacggcggc tttccgggat gggatgcgcg
     3061 aagcgaggcg cagccaatgg tcattcccct tggcaagccg agagacaacg cagaccgccc
     3121 ggataaggcg ccccggactc ggactcgcgc agcgtggagc gggaaggggc gcaagctgcc
     3181 gcaagaaaaa ccgtcaaagg gggcgctcat gctgccgctc ccgcacccca agcttcgctt
     3241 accgtgtcgc agcagcc
//
LOCUS       DATIOW010000122         6611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1582106, whole genome shotgun sequence.
ACCESSION   DATIOW010000122 DATIOW010000000
VERSION     DATIOW010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6611
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582106"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VLL03_03985"
     CDS             complement(<1..259)
                     /locus_tag="VLL03_03985"
                     /inference="COORDINATES: protein motif:HMM:NF024750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HSS46558.1"
                     /translation="MSIIKLTKTAVDSLPYTQNGQTLYFDTELTGFGVRVGRASKVYI
                     AESRLRGKTTRVKIGRHGRLTAEEARSKAKIILGRLEQGLNP"
     gene            534..1733
                     /locus_tag="VLL03_03990"
     CDS             534..1733
                     /locus_tag="VLL03_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HSS46559.1"
                     /translation="MPKSNPPLKGLKVIELGTLIAGPFCTRLLAEFGAEVIKIESPDG
                     GDPLRTWRKLYKGTSLWWLVQARNKKSVTVNLKVSAGQEIVRKLVKDADILVENFRPG
                     ALEKWGLGWDALSQINPGLVMVRLSGYGQTGPYRDQTGFGAIGESMGGIRFLTGYLDR
                     PPVRVGISLGDSLAAMYGVIGALMAVYHREVNGGEGQVVDVALYESVFSLMESLLPEY
                     DMLGFVRERSGASLPGIVSSNTDSCRDGKYVVIGANSDAIFKRMMLSIGRDDLANDPQ
                     LATNAGRVPRTEELDQAIGAWTLRHDLEQVLSVLNQAEVPASKIYSIADIVNDVHYQA
                     REMIQQFRLKDGKPIKLPGIVPKLTETPGATAWLGPELGEHTAEVLTRLGIGDEQQKI
                     LKRQGVI"
     gene            complement(1790..2458)
                     /locus_tag="VLL03_03995"
     CDS             complement(1790..2458)
                     /locus_tag="VLL03_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSS46560.1"
                     /translation="MSDLLPSIELTTGSNPTASVIWLHGLGADGNDFVPVVDELGLPQ
                     RLALRFIFPHAPMQPVTINGGFVMRAWYDIAAADLSLREDEAGVRQSQREIEKLIAHE
                     KNRGIPAGKIVLAGFSQGGAIALQAGLRHPERLAGVLALSAYLSLPSTLAAEANAANA
                     DVPIFMAHGSADPTIPLLLANLSRQHLELQGYKVEWHQYLIPHSLCPEEIRDIGVWLK
                     RVLN"
     gene            complement(2462..3223)
                     /gene="phnE"
                     /locus_tag="VLL03_04000"
     CDS             complement(2462..3223)
                     /gene="phnE"
                     /locus_tag="VLL03_04000"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HSS46561.1"
                     /translation="MKPVQTLLVMVFLLLTALSFNYLSFDLMHFFSSESLVQMKNYIV
                     SFFPPDLSYEYLMRIAQGTMETLAISAIGTGLAAIFGLLLALPAAGRFGLTLRGIARF
                     FLNFLRSVPELVWAAIMVLAAGLGPFAGTLALATHTTGVLGRLFGETLENTPPEPVLA
                     LLHSGSPRMAAFVYGTFPGIFPQLVAYTLYRWENNIRMATILGFVGAGGLGQMLYFSL
                     SLFQQKQAFTVILAMLVLVTIVDATSAWLRGHYVK"
     gene            complement(3220..4041)
                     /locus_tag="VLL03_04005"
     CDS             complement(3220..4041)
                     /locus_tag="VLL03_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSS46562.1"
                     /translation="MLLAQRDPAALPRIALTISALLLLWPGLHFTEFNPVVLFEANNR
                     KTMGQFLGTFFPLNLSPDFLKLVWKSTLETLALATAGTALAMFLAFPAALVVARSLSI
                     SRIGPGPGRLIGQFVRIPMRGILIVLRSVPEIVWALLFVRAVGLGPAAGVLAIAITYA
                     GMLGKVYSEILESGDTRPARVLLESGSGRLTAFFYGILPGTLHELVSYSVYRWECAVR
                     ASVIMGFVGAGGLGQLMELSMRMLSGGEVSTILLVFLLLVLLADAVSAILRKALA"
     gene            complement(4032..4850)
                     /locus_tag="VLL03_04010"
     CDS             complement(4032..4850)
                     /locus_tag="VLL03_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HSS46563.1"
                     /translation="MSLILNQVSLTHAGGNRALANISLELERGERVAIIGPSGAGKTS
                     LLRILATSIRPSAGEVQVLDKNPWRLSRRALKNLRSRIGMVHQAPPLPGRQRVVTAVL
                     AGRLGLWPLWKSLTSLAYPADIAGAQAMLTRLEIADRLFERCDRLSGGQLQRVGIARV
                     LYQQPELILADEPVSSMDPALADRTVGELQNEAASRGITLVASLHAVDLALRWFPRIV
                     GIKSGEAMFDLPPVRVTDAMLQELYASETAVVPTQDNHPLEIEAPGIRRKPICY"
     gene            complement(4868..5710)
                     /locus_tag="VLL03_04015"
     CDS             complement(4868..5710)
                     /locus_tag="VLL03_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate ABC transporter
                     substrate-binding protein"
                     /protein_id="HSS46564.1"
                     /translation="MKAKFLAFCLLLFSFSLHAENVLRVSAIPDEAPTELARKFAPLG
                     KYLEKELGMKVEWTPVTDYAAVVEALAAKKIDLAWLGGFTFVQARLRTGNAIPIAQRA
                     EDEKFTSKFITSVNSGINKLEDLKGRTFTFGSVSSTSGSLMPRYFLLQHGLDPEHDFK
                     RVAYSGAHDATAAWVAAGKAEAGVLNASVWEKLVESGKADTKQVKVFWTTPPYYDYNW
                     TVRGDLDPALVKKLTQAFLKLDPNNPEHKEIMALQRASRFIPTKVENYKNTEEAARSA
                     GLLK"
     gene            complement(5837..6388)
                     /gene="mog"
                     /locus_tag="VLL03_04020"
     CDS             complement(5837..6388)
                     /gene="mog"
                     /locus_tag="VLL03_04020"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HSS46565.1"
                     /translation="MNDELLIGLVSISDRASQGVYQDQGIPALREWFAAALTNPWKIV
                     SRLIPDEQAQIEATLKELVDEAGCHLVLTTGGTGPAPRDVTPEATLSVADKVMPGFGE
                     AMRAVSLKYVPTAVLSRQVGVIRKQALILNLPGQPKAIKETLDGIFAAVPYCIDLIGG
                     PYIETHEAVIKAFRPKSAIRPKP"
     gene            complement(6413..>6611)
                     /locus_tag="VLL03_04025"
     CDS             complement(6413..>6611)
                     /locus_tag="VLL03_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006414361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF615 domain-containing protein"
                     /protein_id="HSS46566.1"
                     /translation="VERWRERLLADEAALAEFLQTYPQADSQRLRALIRNTQQEASVG
                     KPPRNFRLLFDALQELIPDLR"
BASE COUNT         1443 a         1878 c         1964 g         1326 t
ORIGIN      
        1 taggatttag accctgctcc aaacggccga ggataatttt tgctttgcta cgggcctctt
       61 cagcggttaa gcgtccgtga cggccgatct tgacccgggt cgttttgcct ctcagccttg
      121 attcggcaat atagacttta ctcgcgcgcc caacccgcac cccgaacccg gtcagctcgg
      181 tgtcgaagta gagggtctgc ccgttttggg tgtaaggtag agaatcaacc gcggtcttcg
      241 tgagttttat aatggacata gtgagtgcgg gtcctttccg acctactgat gccggaaatt
      301 tgtaggcaat ttgtaggcat taaatcattt gtaggcaatt tgtaggcaac ttttttcaaa
      361 cgacttactg atctatagta cacgttaaat acctacaacg caaggggttt atgatgaatt
      421 caaaattagt aggcattatt caaaaccgga tgattagaat tcgtaatcat taggtcggtg
      481 gttcgaatcc actcatcagc accaccctgt ctgttgttcc atttgggaat cccatgccca
      541 aaagcaatcc gccgctcaaa ggtctgaagg tcatcgagct tggcacgctg atagccggcc
      601 ctttctgcac gcggctgctc gccgagtttg gcgctgaagt gatcaagatc gaatcgcccg
      661 acggtggcga tccgctgcgc acctggagga agctctacaa aggcacgtca ctgtggtggt
      721 tggtgcaggc gcgcaacaag aagtcggtca cggtaaatct gaaagtgtcc gcaggccaag
      781 aaattgtgcg caagctggtc aaagacgccg acatcctggt ggaaaatttc cgccccggcg
      841 ctttggaaaa atgggggctc ggctgggacg cgctctctca aatcaatcct gggttggtga
      901 tggtgcgcct ctccggttac gggcagaccg ggccgtatcg cgatcagacc ggcttcggcg
      961 cgattggcga atcgatgggc gggattcgtt ttctcaccgg ctatctcgac cgccctccgg
     1021 tgcgcgtggg catcagcctg ggcgattcgc tggcggcgat gtacggcgtg atcggggcgc
     1081 tgatggcggt ttaccatcgc gaagtgaacg gcggagaagg gcaggtggtg gatgtcgcat
     1141 tgtatgaatc ggtgttcagc ctgatggaga gcctgcttcc cgaatacgac atgctgggct
     1201 ttgtgcgcga gcgcagcggt gcatcgcttc ccggcatcgt gtcttccaac actgattcgt
     1261 gccgcgacgg caaatacgtg gtgatcggcg ccaattccga cgcgattttc aaacgcatga
     1321 tgctgtcgat aggccgagat gatctggcta acgatccgca gcttgccact aatgccggcc
     1381 gtgtgccgcg caccgaagaa ctggaccagg ccatcggcgc gtggactttg cggcatgatc
     1441 tggaacaagt gctgtcggtg ttgaaccagg cggaagtacc ggcaagcaaa atctacagca
     1501 ttgccgacat tgtcaacgac gttcactacc aagcgcgcga aatgatccag caattccggc
     1561 tcaaggatgg caaacccatc aagctgcccg gcatcgtgcc gaagctgacg gaaacgccgg
     1621 gcgcgacggc gtggctggga ccggagttgg gagaacatac cgctgaagtg ctgacccgtc
     1681 tcggaatcgg cgacgagcag caaaaaattc tcaagcggca gggcgtcatc tgagccgtcg
     1741 ccgactgtcg cggccgcgga acatcggctg tggtgcaagc ggcgcgggct tagttcaaca
     1801 cgcgcttgag ccacactcca atatcccgga tttcttccgg gcacagcgag tgtggtatca
     1861 gatattggtg ccattccacc ttgtagcctt gtagttccag gtgctgccgt gacaagttgg
     1921 cgagcagcaa tgggatggta gggtccgccg aaccgtgcgc catgaagatc ggcacatccg
     1981 cgttggctgc gttcgcctcc gcggccagcg tcgagggcag cgacaaataa gcggagagcg
     2041 ccaacacgcc ggccagccgc tcgggatggc gcaagccggc ttgcagggca atcgcgccgc
     2101 cctgggaaaa gcccgcaagc acgattttgc cggcgggaat gccgcggttt ttttcgtgcg
     2161 cgatcagctt ttcaatttcc cgctgcgatt ggcgcacgcc cgcttcgtct tcgcgcaacg
     2221 acagatcggc ggcggcgata tcgtaccagg cacgcatcac gaagccgccg ttgatggtga
     2281 ccggctgcat cggcgcgtgc ggaaaaatga agcgcagcgc aagccgctgc ggcaacccga
     2341 gctcatctac tacgggtacg aaatcattgc catcggcgcc cagcccatgt aaccagatga
     2401 cactggcagt cgggttgctt cctgtcgtca attcgattga aggaagaagg tcggacacgg
     2461 tttattttac gtaatgcccg cgcagccagg cgctggtcgc atcgacaatt gtaaccagaa
     2521 ctagcatcgc cagaatcacg gtgaacgcct gtttttgctg gaacaggctc aaggaaaaat
     2581 acagcatttg tcccagtccg cccgcgccga caaaacccag tatcgtggcc atacgaatat
     2641 tattctccca gcggtacagg gtgtacgcga caagttgtgg aaagattccg ggaaaagtgc
     2701 cgtaaacaaa cgccgccatg cgcgggctgc cgctgtgcag aagcgccagg acgggttcgg
     2761 ggggtgtatt ttccagggtt tcgccaaaca gccgtcccaa tacgccggtg gtatgtgtag
     2821 cgagggccaa tgtgccggcg aacgggccca ggccggcggc cagtaccatg attgccgccc
     2881 acaccaattc cggcaccgaa cgcaggaagt tcaaaaaaaa cctggcgatg ccgcgcaggg
     2941 tcaagccaaa gcgtcccgcg gcgggaagcg caagcagcaa gccgaaaatg gcggcgagcc
     3001 cagtgccgat ggcagaaatc gccaacgttt ccatcgttcc ctgcgcgatt cgcatcagat
     3061 attcgtaaga aaggtcgggt ggaaaaaatg aaacaatata gtttttcatc tgtaccaatg
     3121 actcagacga aaagaaatgc atcaagtcga acgacagata gttgaagctc aatgccgtaa
     3181 gcagcagaaa caccataacg agcagagtct gcactggttt catgccagcg cctttcgcaa
     3241 aatagcgctc accgcatcgg cgagcagcac cagcagcaga aagaccagca aaatagtgct
     3301 gacttcaccg ccgctcagca tacgcatcga aagctccatc aattgtccca ggccgcccgc
     3361 gccgacaaaa cccatgatca cggaggcccg caccgcgcat tcccagcggt agacgctgta
     3421 ggaaaccaac tcgtgaagcg tgccgggcag gatgccgtag aaaaacgcgg tgagcctgcc
     3481 actgccgctt tccagcagca cccgcgccgg ccgagtatcg cccgactcca gaatttccga
     3541 ataaactttg ccgagcatgc cggcataggt aatggcaatc gccagtacgc ctgcggcggg
     3601 acccaatccc acggcgcgca caaacaagag tgcccatacg atttcaggaa cgctgcgcag
     3661 cacaatcaaa atcccgcgca tcgggatcct gacgaattgc ccaatcaacc gccccggccc
     3721 cgggccgata cgggaaatcg agaggctgcg cgccacaacc aacgctgcag ggaatgccag
     3781 aaacatcgcc agcgcggtgc cggcggtggc aagcgccagc gtttccagtg tggatttcca
     3841 taccagcttg agaaagtccg gagagagatt gagtggaaag aatgttccaa gaaactgccc
     3901 catggtttta cggttattgg cttcgaacag caccacggga ttgaattcgg tgaaatgaag
     3961 ccccggccac agcaaaagca gcgcgctaat tgtgagcgcg attcgcggca gcgccgccgg
     4021 atcgcgttgc gctagtagca tatgggcttg cgccgtatcc cgggcgcttc gatttccaaa
     4081 gggtggttgt cctgggtcgg taccaccgcg gtctcgctcg cgtaaagttc ctgcagcatg
     4141 gcgtcggtca cgcgcaccgg cggcaggtcg aacatcgcct cgccggattt gatgcctacg
     4201 atgcgcggaa accagcgcag cgccagatcc accgcatgca agctcgccac cagcgtgatg
     4261 ccgcgcgagg ccgcttcgtt ttgcaattcg cccacggtgc ggtcggccag cgccggatcc
     4321 attgacgaca ccggttcatc ggcaagaatc agttccggtt gctggtaaag cacccgcgcg
     4381 atccccaccc gctgcaattg gccgccggat aatctatcgc agcgctcaaa cagacggtcg
     4441 gcgatttcca ggcgagtgag catggcttgg gcaccggcga tatcggcggg ataagcgagc
     4501 gaggtgagcg atttccacag tggccacagc ccaagtcggc ccgcgagaac ggcggtcacc
     4561 acacgctgcc ttccgggcag gggcggcgcc tggtgcacca tgccgatgcg gctgcgcaga
     4621 tttttcaacg cgcggcgtga aagccgccag ggatttttgt ccagcacttg cacttctccc
     4681 gcactggggc ggatggatgt ggcgagtatc ctcaggaggc tggtcttgcc cgcgcccgaa
     4741 gggccgatga ttgccacgcg ctcgccgcgt tcaagctcaa ggcttatatt cgcgagcgcg
     4801 cggttgccgc cggcatgggt gaggctgact tggttgagaa tgaggctcaa gtgctacctt
     4861 tacacgttta tttaagtaga cctgccgaac gagcggcttc ttcggtgttc ttgtaatttt
     4921 ccactttggt cgggataaag cggctggcgc gctgcagcgc catgatttcc ttgtgctccg
     4981 ggttgttcgg atcgagcttc aggaaagcct gggtgagttt cttcaccagt gccggatcca
     5041 aatcgccgcg cacggtccag ttgtagtcgt agtagggcgg agtagtccag aatactttta
     5101 cctgcttggt gtccgctttg ccggactcca ccagcttttc ccagaccgaa gcattaagca
     5161 cgccggcttc cgctttgcct gcagcaaccc aggcggccgt cgcgtcatgc gcgccggaat
     5221 aagcgacacg cttgaaatca tgttcggggt cgagaccgtg ctgcaggagg aaatagcgcg
     5281 gcatgaggct gcccgaagtc gaggacaccg aaccaaaagt aaaagtccgg cctttcaggt
     5341 cttccagctt gttgatgccg ctgttgacgg aagtgatgaa cttgctggtg aatttctcgt
     5401 cttccgcgcg ctgcgcgatg ggaatcgcat tgccggtgcg cagccgcgcc tgcacgaagg
     5461 tgaagccgcc cagccacgcc aggtctattt tttttgccgc caacgcttcc accacggcag
     5521 cgtaatcggt gaccggcgtc cactcgacct tcatgccgag ttctttttcc agatattttc
     5581 caagaggagc gaacttgcgc gcaagttcgg ttggggcttc atcggggatc gccgaaacgc
     5641 gcagcacgtt ttcggcgtgg agggaaaatg agaaaagcag caaacaaaag gcaagaaatt
     5701 tggctttcat ctcagggccc tttcttgaaa tgggtatagg ttttgaaaga cacgattgtc
     5761 ctcacggctt tgagttgcgg aaaagtcatc ctagcatcgg caataatcgc aggcaacagc
     5821 gaaaaacgca gtgcaatcag ggcttggggc gaatcgcgga tttggggcgg aacgccttga
     5881 tgacggcctc gtgggtttcg atatacggcc cgccgatgag atcgatacaa tacggaacgg
     5941 cggcgaaaat tccgtcgagt gtttccttga tggctttcgg ctggccggga agattgagta
     6001 tcagcgcttg tttgcggatg acgccgacct gacgcgacag aacggcggtg ggcacgtatt
     6061 tcaggcttac cgcgcgcatc gcttcgccga atcccggcat caccttgtcc gcgaccgaca
     6121 gcgtggcttc gggcgtgaca tcgcgcggcg ccggcccagt gccgccggtg gtgagcacca
     6181 gatggcagcc cgcttcatcc accagttctt tcagcgtcgc ctcgatttgc gcttgctcgt
     6241 cggggatgag gcgcgacact attttccagg gattggtcag cgccgcggca aaccattccc
     6301 gcagcgcagg aatgccctga tcctgataca cgccttgtga agcgcggtcg ctgatggaga
     6361 ccaggccgat tagaagctca tcattcatga catgtaagta tagcggatag cctcagcgca
     6421 gatcgggaat cagttcctgc aaagcgtcaa acaacaagcg gaagtttcgc ggcggcttgc
     6481 cgacagaggc ttcctgttgc gtgttgcgaa tcagcgcacg cagccgctgg ctgtcggcct
     6541 gcggataggt ttgcaaaaat tccgcgagcg cggcttcatc ggcaagcaac cgctcacgcc
     6601 agcgctctac c
//
LOCUS       DATIOW010000123         6335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_871597, whole genome shotgun sequence.
ACCESSION   DATIOW010000123 DATIOW010000000
VERSION     DATIOW010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871597"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VLL03_04030"
     CDS             <1..263
                     /locus_tag="VLL03_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HSS46567.1"
                     /translation="ANRDAESWLKAVMAPMETQVREHQLQLKRRLESIKHIHKATDTL
                     EDRITELEEQERQIKHQLDKLKQVSHKIAVRLFFDEPREMAA"
     gene            complement(300..566)
                     /locus_tag="VLL03_04035"
     CDS             complement(300..566)
                     /locus_tag="VLL03_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HSS46568.1"
                     /translation="MALMITDECINCDVCEPECPNGAISQGEEIYVINPRLCTECVGH
                     YDRPKCVEVCPVDCIPLNPDYVESQKQLHEKYLVLQTQKNASSA"
     gene            complement(584..1066)
                     /gene="coaD"
                     /locus_tag="VLL03_04040"
     CDS             complement(584..1066)
                     /gene="coaD"
                     /locus_tag="VLL03_04040"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015422075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HSS46569.1"
                     /translation="MNKAVYPGSFDPITRGHEDLVRRAASIFDAVIVAVADSRDKRPF
                     FTLKERVDMAKQVLTGYQNVQVLGFSGLLMDFLRVQKATIILRGLRAVSDFEFEFQMA
                     GMNRILYPDVETIFLTPSEQYMFISATMVREIATLGGEVGPFVQPLIAKKLSAKIKKP
                     "
     gene            complement(1059..1604)
                     /gene="rsmD"
                     /locus_tag="VLL03_04045"
     CDS             complement(1059..1604)
                     /gene="rsmD"
                     /locus_tag="VLL03_04045"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HSS46570.1"
                     /translation="MRRGRVRIIGGEWRSRLIEFPALPDLRPTPDRVRETLFNWLGQD
                     LTGMTCLDLFAGSGALGFEALSRGSQKVVMVEKHRVAFQALRENAHRFKAQNLELVHA
                     DALQFLKADRNVYDMVFLDPPFRDDYLPLLLAELPARLVPEGRVYVESGDDRDEPGWE
                     TWRRARAGQVRYQLLKRKQHE"
     gene            complement(1617..2942)
                     /locus_tag="VLL03_04050"
     CDS             complement(1617..2942)
                     /locus_tag="VLL03_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSS46571.1"
                     /translation="MINVLKWRSLVLLGVFAGAGPAFAILPIQHWQTSTGARVYFVES
                     HELPMLDISIDFAAGSSRDSLEKSGLASLTRHLLSLGADGLSEDEIAEKLADVGAQLG
                     GRIDQDRAGMTLRTLSSARERDQALDVLSRIMQRPEFPDKVLDREKTRMAAMLKEAAT
                     KPESIAERAFYSLLYGSHPYALRTSGEVDSLESLKRDDLVRFYRAYYTANDAVVAIIG
                     DVSRETAQEIAEKLTAKLPEAASPLPVLPQVPLPAQAQNREIQHPATQSHILIGYPGI
                     KRDDPDYFALFVGNYILGGGGFASRFVEQVRQKRGLVYSVYSYFMPLKEQGPFQIGLQ
                     TKKEQADEALKVVRETLRDFIARGPTEQELKAAKQNLVGGFPLRIDSNKKILEYLSVI
                     GFYRLPLTYLDDFVPRVENVTVAQIKSAFQRRIDPGHMVTVVVGAPGAG"
     gene            complement(2935..4323)
                     /locus_tag="VLL03_04055"
     CDS             complement(2935..4323)
                     /locus_tag="VLL03_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSS46572.1"
                     /translation="MNDIARVLKCSSFAAAFLLVSLFPCAARAVTAEHTLKNGLKVIV
                     KEDHRSPVVVSMVWYKVGSMDEVNGTTGVAHVLEHMMFKGTKKVPSGEFSKIIAQAGG
                     RDNAFTSKDYTAFFQQLHKSKLPLAMKLEADRMQSLELSRQEFDKEIKVVMEERRWRT
                     DDKPQALVYEQLLAATFEAHPYRRPVIGWMNDLENMRVDDAARWYRTWYAPNNATLVV
                     VGDVKLNEVFNLARRYFGAIKPKVLPMRKPQEEPQQTGIRRVTVKAPAKLPYLMMSYH
                     APVLRDTERDWEPYALEILGGVLDGNESARLNRSLVRETQVASSTGVSYDSAERGPGL
                     FYLDGVPSEGKSVADLETAIRGELQKIINDGVTNEELNRVKAQVIANEVFERDSVMAQ
                     ARQIGEWEMAGLSYKTLDILVDKLKAVSAEQVQAVAKKYFNDDNLTVAALDPQPLENK
                     KPLKAPEGLRHD"
     gene            4417..5367
                     /gene="ftsY"
                     /locus_tag="VLL03_04060"
     CDS             4417..5367
                     /gene="ftsY"
                     /locus_tag="VLL03_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HSS46573.1"
                     /translation="MFSFLKQDKTENGASSWTERLKQGLARTRNQLGKQLASVFGGHG
                     KIDQALYEELESVLLLSDVGVSATRHLLEQLRVRVEHDKLQDATKLKQALQEALLELL
                     APLEKPLDTASHRPFIIMVAGVNGSGKTTSIGKLANYFQTQGNSVLLAAGDTFRAAAR
                     EQLMAWGERNKVQVIAQQSGDPAAVIFDAIHAARARNIDIVLADTAGRLPTQLHLMEE
                     IKKVKRVIAKADPSAPHETLLVLDANTGQNALAQVKAFDTALGLTGLVLTKLDGTAKG
                     GAIAAIAREKPIPMRFIGVGEGLDDLRPFSAKEFVAALFE"
     gene            5364..6017
                     /locus_tag="VLL03_04065"
     CDS             5364..6017
                     /locus_tag="VLL03_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSS46574.1"
                     /translation="MISASVVCKRYPGGQEALRHLSFKIEAGELVGVTGHSGAGKSTL
                     LKLLAAIERPTSGSLIVNGQNTGSLRAAAIPYLRRHFGLVFQDQKLLFERTVFENVLL
                     PLAITGFDRRESAKRARAALDKVGLLNKEKALPITLSGGEQQRLCIARAIVSRPAILL
                     ADEPTGNLDSAYATDIMELFKSFNQVGVTVIIASHDETMLAKLNARLMKLRQGELIQ"
     gene            6014..>6335
                     /locus_tag="VLL03_04070"
     CDS             6014..>6335
                     /locus_tag="VLL03_04070"
                     /inference="COORDINATES: protein motif:HMM:NF037183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HSS46575.1"
                     /translation="MKAWFVQHWETLWLSAKRLTRNPFANLLNIGVIGIALSLPCGLY
                     VLLANLQGLTKQVANEPQISLFLNTQAAPADATQIETLLKNHPGVKNFEFVPKDRALR
                     ELQKA"
BASE COUNT         1346 a         1935 c         1746 g         1308 t
ORIGIN      
        1 tcgccaaccg cgacgccgaa agctggttga aggcggtaat ggcgccgatg gaaacccagg
       61 tgcgcgagca tcagctgcaa ctgaaacgac ggctggagag catcaagcac atccacaagg
      121 cgaccgacac gctggaagat cgcattaccg agctggagga gcaggaacgc cagatcaaac
      181 accagctcga caagctcaag caagtctcgc acaagatcgc cgtcaggctg ttcttcgacg
      241 agccgcgcga aatggccgcc tgaggcggcg ctgtttccag ttgttacatc agctgaaatt
      301 taagcggaag aagcgttttt ttgcgtctgc agcacgagat acttctcgtg cagctgtttc
      361 tgactttcga cgtaatccgg attgagcgga atgcagtcca ccgggcacac ttccacgcat
      421 ttcggccggt cgtaatgccc cacgcactca gtgcagagcc ggggattgat gacgtaaatt
      481 tcctcgccct gcgaaatcgc gccgttcggg cactccggct cgcacacgtc gcaattgatg
      541 cattcatcgg tgatcatcag cgccatggaa tttgctcact tcatcacggt ttcttgattt
      601 tggcactcaa cttcttggca atcaagggct gcacgaacgg tcccacctcg ccgcccagag
      661 tcgcgatctc gcgcaccatg gtagcggaaa tgaacatgta ctgctcggaa ggcgtgagaa
      721 aaatcgtttc cacgtcggga tacaaaatgc ggttcattcc cgccatctgg aattcgaact
      781 cgaaatcgga aacggcgcgc aagccgcgca ggatgattgt cgccttttgc acgcgcaaga
      841 aatccatcag caaaccggaa aatccaagca cttgcacgtt ctggtagccc gtcagcacct
      901 gtttggccat gtccacccgc tccttcagcg tgaaaaacgg ccgcttgtcg cggctgtcag
      961 ccaccgccac gatgacggca tcgaagatgc tggcggcgcg gcgcaccagg tcttcatggc
     1021 cgcgggtgat gggatcgaaa ctgcctgggt agactgcctt attcatgttg tttccgtttc
     1081 agcaattggt aacgcacctg gccggcccgc gcccggcgcc acgtctccca accgggctca
     1141 tcacggtcgt cgccgctttc cacatacacc cgcccttccg gaaccaggcg cgcaggcaac
     1201 tcagcaagca ggagcggcag ataatcgtca cggaacggcg gatcgagaaa aaccatatcg
     1261 tatacatttc ggtcggcttt caggaattgt agcgcatcgg cgtgcaccag ttccaggttt
     1321 tgcgccttaa acctgtgcgc gttttcacgc agcgcctgaa acgccacgcg gtgcttttcc
     1381 accatcacca ccttttgcga gccgcgggat aaggcttcga agcccagcgc gccactgccg
     1441 gcaaacaaat cgagacaggt catgccggta aggtcttggc caagccagtt gaacagggtt
     1501 tcgcgcacgc ggtcgggagt ggggcgcaaa tcgggcagcg ccggaaactc gataagacgg
     1561 ctgcgccatt cgccgccgat gatgcgcact ctgccgcgcc gcatgagaaa acgccgtcag
     1621 cccgcgcctg gcgcgcccac caccaccgtg accatgtgcc cgggatcaat gcgccgctgg
     1681 aaggcgcttt tgatttgcgc cacggtcaca ttctcgacgc ggggaacaaa atcgtcgaga
     1741 taagtcagcg gcagacggta aaagccgatt acggatagat actcgagaat tttcttgttg
     1801 ctgtcgatgc gcagcggaaa accgccgacc agattctgct ttgccgcctt gagctcctgt
     1861 tcggtcggcc ccctggcgat gaaatcacgc agcgtctcgc gcaccacttt aagcgcctcg
     1921 tccgcctgct cctttttggt ctgcaagcca atctggaacg gaccctgctc tttgagcggc
     1981 ataaaataac tgtaaacgct gtacaccagc ccgcgttttt gccgcacttg ctcgacgaag
     2041 cgcgaagcga acccgccgcc gccgagaata tagttgccga caaacagcgc aaaataatca
     2101 gggtcgtcgc gcttgatgcc cggatagccg atgagaatat ggctttgcgt cgccggatgc
     2161 tgaatctcac ggttttgcgc ctgtgccgga agcgggacct gcggcagcac cggcaagggc
     2221 gaagccgctt cgggtagttt cgcggtgagt ttttccgcga tttcctgcgc ggtctcgcgg
     2281 cttacatcgc cgataatcgc caccaccgca tcgtttgcgg tgtaataagc gcgataaaac
     2341 cgcactaaat catcgcgctt gagcgactcc aggctgtcca cttccccgga agtacgcagc
     2401 gcatacgggt ggctgccgta gaggagcgag tagaacgcgc gttcggcgat gctttccggc
     2461 ttggtcgccg cttctttcag catcgccgcc atgcgcgtct tttcccggtc cagcacctta
     2521 tcgggaaact cgggccgctg catgatgcgc gacaaaacat cgagcgcttg atcgcgctcg
     2581 cgcgcgctgc tcaaggtgcg cagcgtcatg ccggcgcggt cctgatcgat gcgcccgccc
     2641 agctgcgccc cgacatcagc gagcttttcg gcgatttcgt cttcggaaag tccgtcggcg
     2701 cccaggctca gcaggtgccg ggtgaggctc gccaagcccg atttttccag gctgtcgcgg
     2761 ctgctgccgg cggcgaaatc aatgctgata tcgagcatcg gcagttcatg gctttccaca
     2821 aaatagaccc gagcgccggt tgacgtctgc caatgctgta tcggcaggat ggcaaaagcg
     2881 ggtccggctc cggcgaatac gccaagcaaa accaagctgc gccactttaa aacgttaatc
     2941 atgtcttaat ccttccggcg ccttgagcgg ctttttgttt tcgagaggct gcggatcgag
     3001 tgccgcgacc gtgaggttgt cgtcgttgaa atattttttt gccaccgcct gtacctgctc
     3061 cgcgcttacc gctttcaact tgtccaccag aatatccagc gttttatacg acagccctgc
     3121 catttcccac tcgccgattt gccgcgcctg ggccatgacc gaatcgcgct caaatacttc
     3181 gttggcgatg acctgggcct ttacccggtt cagttcctca ttggtcacgc cgtcattgat
     3241 gattttttgc agctcgccgc gtatcgctgt ttccaaatcg gcgacacttt taccttcgct
     3301 gggaacaccg tccaagtaaa acaggcccgg accgcgttca gcgctgtcat aactcacccc
     3361 ggtggaagag gcgacctggg tttcgcgcac caaggaacgg tttaaccgcg ccgactcgtt
     3421 gccatcgagc acgccgccaa gaatctccag cgcgtaaggc tcccagtcac gctcggtatc
     3481 gcgcagcacc ggcgcgtggt agctcatcat gaggtaaggc aatttggctg gcgctttcac
     3541 cgtgacgcgg cggatgccgg tttgctgcgg ttcttcctgc ggtttgcgca tcggcagcac
     3601 tttgggtttg atggcaccaa aatagcgccg ggcgagatta aacacttcat tcagcttcac
     3661 atcacccacc accaccagtg tcgcattatt aggcgcatac caagtacggt accatcgcgc
     3721 cgcatcgtcc acccgcatgt tttccagatc gttcatccag ccaatgaccg gcctgcggta
     3781 ggggtgggct tcgaaagtcg ccgccagcaa ctgctcataa accagcgcct gaggcttgtc
     3841 gtcggtgcgc cagcgccgct cctccatcac cacctttatt tctttatcaa attcctgtct
     3901 ggaaagctcc aaactctgca tgcggtccgc ttccagtttc atggcaagcg ggagctttga
     3961 cttgtgcagt tgctggaaaa aggcagtgta atccttactg gtaaaagcgt tgtcgcgccc
     4021 gccggcttgg gcgatgattt tggaaaattc gccgctcggc acctttttcg tgcccttgaa
     4081 catcatgtgc tccagcacat gcgcgacacc ggtggtgccg ttcacttcat ccatgcttcc
     4141 gaccttgtac cacaccatcg acaccaccac cggcgagcgg tggtcttcct tgacgatcac
     4201 ctttaggccg tttttcagcg tgtgttccgc ggtgacggcg cgggcggcgc aggggaacaa
     4261 gctgacgagt agaaaagctg ctgcaaaact tgaacactta agaacgcggg caatatcatt
     4321 cattattttt ggcctacgat gggttgactt cgaatagaat tatacccgga ttgcatttct
     4381 tcattccggg cacgcgattt gtgacaacag gcaggcatgt ttagtttctt aaagcaagac
     4441 aaaactgaaa acggcgcctc gagctggact gagcgcttga agcagggcct tgcacgcacc
     4501 cgcaaccagc tgggcaaaca actggcgagc gtgtttggcg gccacggcaa aattgaccag
     4561 gcgctgtacg aggaactgga aagcgttctg ctcctgtccg acgtcggcgt ttccgcaacc
     4621 cgtcatctcc tcgaacaatt gcgcgtacga gtcgaacacg acaagctaca ggatgcaact
     4681 aaactcaagc aggcattgca agaggctttg ctggaattgc tcgcgccgct tgagaaaccg
     4741 ctggataccg cttcccacag acctttcatc atcatggttg ccggcgtgaa cggcagcggc
     4801 aagactactt cgatcggtaa gctcgccaac tattttcaaa cgcagggcaa ttcggtcttg
     4861 ctggctgcgg gggacacctt ccgcgcggcg gcgcgcgagc agttaatggc ctggggcgag
     4921 cgcaacaagg tccaggtcat cgcacagcaa agcggcgatc cggcggcggt gatattcgat
     4981 gcgatccacg ccgcccgggc acgcaatata gatatcgtgc ttgccgacac cgcgggccga
     5041 ctgcccacac agctgcatct gatggaggaa atcaagaaag taaagcgcgt gattgccaag
     5101 gcggatcctt ccgcgccgca tgaaaccctg ctggtactgg acgccaacac cgggcaaaat
     5161 gcactcgcac aggtcaaagc gtttgacacg gcgcttgggc tcaccgggct cgtcctcacc
     5221 aagctcgacg gcactgccaa aggcggagcc atcgccgcca tcgccaggga aaagcctatt
     5281 ccgatgcgct tcatcggcgt cggcgaagga ctggacgatt tgcgtccctt ctctgcaaaa
     5341 gaatttgttg cggcgctctt cgaatgattt ccgccagcgt ggtctgcaag cgctaccccg
     5401 gcggccagga ggcactgagg cacttaagct tcaagattga agcgggcgag ctggtgggag
     5461 tcaccggtca ttcgggcgcc gggaaaagta ccctgctcaa actcctcgcc gccatcgagc
     5521 ggcccaccag cggcagcctt atcgtcaacg gccagaacac gggctcgctg cgcgcggcgg
     5581 cgattcccta tctgcgccgt catttcggcc tggtgtttca ggaccagaaa cttctgtttg
     5641 agcgcaccgt gtttgaaaac gtgctgctgc cgctcgcgat caccggtttc gaccgccgcg
     5701 aaagcgcgaa acgcgcgcgc gcggctctcg ataaagtggg gctgctcaac aaggaaaaag
     5761 ctctgccgat cacgctttcc ggcggcgagc agcagaggct gtgcatcgcc cgcgccatcg
     5821 tcagccgccc ggcaatactc ctcgccgacg agcccaccgg caatctagat tccgcttatg
     5881 ccaccgacat catggaactt ttcaaatcgt tcaaccaagt cggcgtcacc gtgattatcg
     5941 cgagccacga tgaaaccatg cttgccaaac tcaatgcacg gcttatgaaa ctccgccaag
     6001 gggaactcat acaatgaaag cctggtttgt ccagcactgg gaaacgctgt ggctttcggc
     6061 aaagcgcctt actcgtaatc cgtttgcaaa cctgcttaac atcggcgtca ttggcatcgc
     6121 gctgagcctg ccctgcggcc tgtacgtgct gctcgccaac ctgcagggac tcacgaagca
     6181 ggtggcgaac gaaccgcaga tcagcctctt tttgaacacc caggccgccc ctgccgatgc
     6241 cacgcaaatc gaaacccttc tcaaaaacca ccctggggtg aaaaacttcg aatttgtgcc
     6301 gaaagaccgg gcgctgcgag agctgcaaaa agccg
//
LOCUS       DATIOW010000124         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1583628, whole genome shotgun sequence.
ACCESSION   DATIOW010000124 DATIOW010000000
VERSION     DATIOW010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583628"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VLL03_04075"
     CDS             <1..697
                     /locus_tag="VLL03_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46576.1"
                     /translation="KPQLGLIQLGFLLGSAGLHTIYYLLLDKGYRAGDLSLVYPLARG
                     SGPLIAIVAAVLLLGERPSGSVLAGAVLIGIGAFLLTGDPRKLKENGALHAVRFALMT
                     GAVIAAYTLWDKFAVSALLIPPLIQDWGTNCGRTLLMLPLARRHSTSIHTAWKNYRKE
                     IILISILGPLSYIMILSAMVFTPVSYVAPAREISILIAAVMGAHFLAEGDLPRRALAA
                     TAMVIGVIALALG"
     gene            768..1001
                     /locus_tag="VLL03_04080"
     CDS             768..1001
                     /locus_tag="VLL03_04080"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HSS46577.1"
                     /translation="MRPAYTEIYRPNDQLEVMMLKMVLEREGVNYFITNEHLNSILPP
                     AGLGDMRLMVETGRAQQCVEILRDELHYLQEPK"
     gene            complement(1071..1853)
                     /locus_tag="VLL03_04085"
     CDS             complement(1071..1853)
                     /locus_tag="VLL03_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HSS46578.1"
                     /translation="MKHSLALILCLLLGGCFLFPDKEDPGNIPPSKLFADAMDDMDAG
                     NFEQAIKKFEILESRYPYGKYAEQAQLEVAYAYYKQDDAASAAAACDRFIKLHPTHPS
                     VDYAYYLKGLVNFNENLGLIANLVNEDMAERDPKSARDSYEAFKELVTRFPNSIYAEN
                     ASARMRFLLNSLAQHEIHVARYYMKRKAYVAAANRSQLVLQNYPQTPAIEEALLIMVQ
                     AYDAMGLNQLRDDAERVMRYNFPDSKYLSKVGDATKSWWKLW"
     gene            2001..>2407
                     /locus_tag="VLL03_04090"
     CDS             2001..>2407
                     /locus_tag="VLL03_04090"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HSS46579.1"
                     /translation="MIPPDCAGLRLDQALQRLLPEFSRNRLQTWIREKRVWQNGAPVV
                     AKRKVWGGEKISVAVAPSAETLAHLPEDIPLDIVHEDESLLVINKPPGLVVHPGSGNW
                     RGTLLNALLKHSPGLAEIPRAGIVHRLDKDTSGL"
BASE COUNT          488 a          692 c          688 g          539 t
ORIGIN      
        1 aaagccgcaa ctcggcttaa tccagctggg tttcctgctc ggcagcgccg gcctgcacac
       61 gatttactat ctgctcctcg acaaaggtta ccgcgctggc gatctgtcgc tggtctatcc
      121 tctggcgcgc ggcagcggcc cgctgattgc catagtggcg gcggtgctgc ttctcggcga
      181 gcggccgagc gggagcgtgc tggcgggagc ggtgctgatc ggcatcggcg catttctgct
      241 caccggcgat ccgcgcaaac tgaaggaaaa cggggcattg cacgcagtgc gtttcgcgct
      301 catgaccggc gcggtgatcg ccgcctatac cctgtgggac aagttcgcgg tgagcgctct
      361 gttgattccg ccgttaatac aagactgggg caccaactgc ggacggacgc tgctgatgct
      421 tccgcttgcc agacgccatt cgaccagtat ccatacagcc tggaaaaact atcgcaagga
      481 aatcatactg atttcgattc tggggccgct ctcgtacatc atgattctga gcgcaatggt
      541 gttcacgccg gtgagctacg tggcgccggc gcgtgaaatc agcatcctaa ttgccgcagt
      601 gatgggcgcg cacttcctgg cggaaggcga tttgccgcgc cgcgcgctcg ccgccaccgc
      661 catggtcatc ggcgtcatcg cgctggcgct gggttgacgc gggtcggttt tgcttttaac
      721 ctccgcttgg cctactctag ttactttaca ccggcttcat tgaaatcatg cgccccgcct
      781 acaccgaaat ctaccggccg aacgaccagt tggaagtaat gatgctgaaa atggtgctgg
      841 aacgcgaagg tgttaattat ttcatcacca acgaacactt gaacagcatt ctgccgccag
      901 ccggcctggg tgacatgcgg ctcatggtgg aaaccggcag ggcgcagcag tgcgtcgaga
      961 ttctgcgtga cgaactccat tatttgcagg aaccgaaatg acctcatggc ccggaatcat
     1021 gttcggctcg ccttattcct aacctctttc cttcatcctg actttctcgg ttaccacagc
     1081 ttccaccaag atttggtggc gtcgcctact ttggaaagat atttgctgtc gggaaagttg
     1141 tagcgcatga cgcgttcggc gtcgtcgcgc aactggttaa ggcccatcgc atcgtaagcc
     1201 tggaccatga taagcagcgc ctcttcgata gccggcgttt gcggatagtt ctgcaggacc
     1261 agctggctgc ggtttgccgc cgcgacataa gccttgcgtt tcatgtaata gcgcgccacg
     1321 tggatttcgt gctgtgccag gctgttcaac aaaaaacgca tgcgcgcgct ggcgttttcg
     1381 gcgtatatgc tgttgggaaa acgcgtgacc agttccttga acgcttcgta ggaatcgcgc
     1441 gccgacttgg gatcgcgttc cgccatatcc tcgttcacaa ggttggctat taatcctaga
     1501 ttctcattga aattgacgag tcccttgagg taatacgcgt aatccacgct gggatgagtc
     1561 ggatgcagct tgatgaagcg gtcacacgcc gccgccgcgg aggccgcatc atcctgcttg
     1621 taataggcgt aagccacttc gagttgggcc tgctccgcat atttgccgta aggatagcgc
     1681 gattccagga tttcaaattt cttgatggcc tgctcgaaat tgccggcatc catatcgtcc
     1741 atggcgtcgg cgaacagctt gctgggagga atgttgcccg ggtcttcctt gtccggaaac
     1801 aggaagcagc cgcctaacag caggcacagg attaaagcta aactatgttt catatccatt
     1861 acgtgaagag taaggagtaa ggaatgagga gaaaaatcag tgttcggtcc gtggtcgagg
     1921 ggcctcacac cttacccttt acccctcacc ccttaccgcc taccgattat aacccaagtg
     1981 cagcgcctca cctcgaactg gtgatcccgc cggattgcgc cggcttgcgc ctggaccagg
     2041 ctttgcaacg tttgttgccg gaattttccc gcaaccggct gcaaacctgg atacgcgaaa
     2101 aacgggtgtg gcaaaacggc gcgcccgttg tcgccaagcg caaggtatgg ggcggagaaa
     2161 aaatcagtgt cgccgtcgcg ccttccgctg aaacactggc gcatttgccg gaggatatcc
     2221 cgctcgacat cgtccatgaa gatgaatccc tgctggtcat caacaaaccg ccggggctgg
     2281 tggttcatcc gggcagcggc aactggcggg gaacgttgct caatgcgctg ctcaagcact
     2341 cacccgggct tgccgaaatc ccgcgcgccg ggatcgtgca ccggttggac aaggacacca
     2401 gcggtct
//
LOCUS       DATIOW010000125         4427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1481034, whole genome shotgun sequence.
ACCESSION   DATIOW010000125 DATIOW010000000
VERSION     DATIOW010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481034"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..542
                     /gene="rpsI"
                     /locus_tag="VLL03_04095"
     CDS             150..542
                     /gene="rpsI"
                     /locus_tag="VLL03_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HSS46580.1"
                     /translation="MIGEHNYGTGRRKSAVARVFLKPGKGVITVNGKLVDEFFSRQTG
                     RMVVRQPLAITNHLSSFDIKVNVHGGGESGQAGAVRHGITRALIEYDAALKPALSKAG
                     LVTRDAREVERKKVGLHKARRRKQYSKR"
     gene            658..1686
                     /gene="argC"
                     /locus_tag="VLL03_04100"
     CDS             658..1686
                     /gene="argC"
                     /locus_tag="VLL03_04100"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HSS46581.1"
                     /translation="MLKVGVVGGTGYTGVELLRILAHHPKVSVSAITSRGEAGMEVAE
                     LFPSLRGMVDLKFSDPAKAKLDSCDVVFFATPNGIAMRQTKALLDAGVRVIDLAADFR
                     LKDAAVWEKWYGTPHACPELLSEAVYGLPEVNRDKIRQARLVANPGCYPTAVQLGFLP
                     LIEAGVIDIERLIADAKSGVSGAGRKAELDALFSEASDNFKAYGVPGHRHLPEIIQGL
                     AAAAGKKVGLTFVAHLTPMIRGIHATLYARLTRETELQALYEKRYAAEEFVDVLPAGS
                     HPDTRSVRGANMCRIAVHRPQGGDTVVALSVIDNLVKGAAGQAVQNMNIMFELPESAG
                     LHHAALLP"
     gene            1690..2427
                     /locus_tag="VLL03_04105"
     CDS             1690..2427
                     /locus_tag="VLL03_04105"
                     /inference="COORDINATES: protein motif:HMM:NF042896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HSS46582.1"
                     /translation="MYKALKRKFGISAPRMAVHTHIPWYWRWLMLTVLIALVLSLAWW
                     MYESGQKFAGFDRFKVETDLATQTELNSKLHEENATLRDQVAISERQLQIERSTYGDL
                     AKQVKALSDENNSLKEDLAFFQSLLSAGSKQEVSIYRFNVERDALPGEYRYRLLLLQM
                     GPRQKEFQGKLQFIVDVEHDGERSVITIPNENDAHLQAYNVSFKYYQRVEGSFRMEPG
                     AVAKSVQVRVFERGVSEAKLTQTVDLS"
     gene            2443..2850
                     /locus_tag="VLL03_04110"
     CDS             2443..2850
                     /locus_tag="VLL03_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HSS46583.1"
                     /translation="MFGKKPSKPQNRIDCLIGVGTSIEGNINFSGGLRVDGHVRGNVV
                     ANGEDPSTLVLSEQARIEGEIRVSHVVINGNVIGPVHAGEYIELQSKSNVTGDVYYKT
                     LEIQLGAVVQGRLVYQDGENAEKVLVFKPATGD"
     gene            2912..3262
                     /gene="erpA"
                     /locus_tag="VLL03_04115"
     CDS             2912..3262
                     /gene="erpA"
                     /locus_tag="VLL03_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HSS46584.1"
                     /translation="MNTMTEIPAPLVFTDNAAGKVKQLLEEEGNPDLKLRVFVTGGGC
                     SGFQYGFTFDEVVNEDDTSMQKNGVTLLIDPMSYQYLAGAEIDYQEGAEGAQFVIKNP
                     NATSTCGCGSSFSA"
     gene            complement(3305..4402)
                     /locus_tag="VLL03_04120"
     CDS             complement(3305..4402)
                     /locus_tag="VLL03_04120"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020425005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HSS46585.1"
                     /translation="MSTRQLYAGLMSGTSLDGIDAILADFGNTPWQLREHVYRSYPDD
                     LRSRLLLLHRPQANELHETMLAANELTRCYAAAIAELLTQAGVKAQEITAIGCHGQTV
                     RHAPQLGYSLQLCNGALLAELTEITVVCDFRSRDIAAAGQGAPLVPAFHAALSLGSNA
                     HRVIVNIGGMANLTNLPPESPASGFDCGPGNMLLDAWCQKHTGQRYDADGDWAMSGQI
                     IPELLQRLLIHPFFVESPPKSGGREQFNLQWLNGFLSGKEAPQDVQATLLALTVQGIA
                     QAIQKYCRGAQEIYFCGGGARNKALVKNLRDALPRVRTDVTDALGINAQTLEAYAFAW
                     LAWQTLQGKPGNMPQATGAKGPRVLGAIYKK"
BASE COUNT          974 a         1227 c         1332 g          894 t
ORIGIN      
        1 cttcgctgtg cccgctgacc tcgaacctca tcggcttcca gacaccatct gcatgttacg
       61 ccatgctgaa gaaactcaag gtctacagcg gtgaacggca cccgcacacc gcgcagcaac
      121 cccggatact ggaaatttaa ggaagactca tgatcggaga acacaactat ggcaccggcc
      181 ggcgcaaaag cgcggtggcc cgcgtgtttc tcaagcccgg caagggagtg attaccgtga
      241 acggcaagct ggtggacgag tttttctcgc gccagaccgg acgcatggtg gtgcgccagc
      301 cgctcgccat caccaatcat ttgtcgagct tcgacatcaa ggtcaacgtg catggcggcg
      361 gtgaatccgg ccaggccggc gcggtgcgcc acggcattac ccgcgcgttg atcgagtacg
      421 atgccgcgct caaacccgcc ttaagcaagg cgggcctggt gacgcgcgat gcgcgcgaag
      481 tcgagcgcaa aaaagtgggc ttgcacaagg cgcgacgacg caaacagtac tccaaacgct
      541 gattgtgcag ttttttcatt catgaaaaag ccgcccacct gggcggtttt tttattgcct
      601 aatcggggca agctgattac catcaacacc tatgtcagtg gaagtgacgg gaaaatcatg
      661 ctgaaagtcg gagttgtcgg cggcaccggg tacaccggcg tggaactttt gcgcattctt
      721 gctcaccatc cgaaagtgag cgtgagcgcg attacttcgc gcggcgaagc ggggatggag
      781 gtggccgagc ttttccccag cctgcgcgga atggtggatt tgaaattttc cgacccggcc
      841 aaggccaagc tcgattcctg cgatgtggtg ttctttgcca cgcccaacgg cattgccatg
      901 cggcagacga aagcgttgct ggatgccggc gtgcgggtga ttgatctcgc cgcggatttc
      961 cgtcttaagg acgcagctgt ctgggaaaaa tggtacggaa cgccgcatgc ctgcccggag
     1021 ctgttgagcg aggcggtgta cggtcttccg gaagtcaatc gcgacaagat ccgacaggcg
     1081 cggctggtcg ccaaccccgg ctgttacccc accgcggtgc agctgggttt tctgccgctc
     1141 atcgaagccg gcgtgattga tatcgagcgc ctgattgcgg atgccaaatc cggcgtctcg
     1201 ggcgccgggc gcaaggcgga gctggatgcg ctgttttccg aagcgtccga taatttcaag
     1261 gcttatggcg tgccgggcca ccgccacctt cctgaaatca ttcagggcct cgcggcagcg
     1321 gcgggcaaga aagtcgggct gactttcgtt gcgcatctca ccccgatgat acgcggcata
     1381 catgccacgc tttacgcacg gcttacgcgc gaaaccgagc tgcaggcact ctatgagaaa
     1441 cgctatgccg cggaagaatt cgtggatgtg ctgccggcgg gcagccaccc cgacacgcgc
     1501 tcagtgcgcg gagcgaacat gtgccgcatc gcggtgcacc gtccgcaggg cggcgacacc
     1561 gtggtggcgc tgtcggtcat cgacaatctg gtcaaaggcg cggccggaca ggcggtgcag
     1621 aacatgaaca tcatgtttga acttcccgaa tcggccggct tgcatcatgc cgcgctatta
     1681 ccttagaaaa tgtacaaggc gctgaaacga aaattcggca tttcagcacc gcggatggcg
     1741 gtgcacacgc atattccctg gtactggcgc tggctgatgc tgactgtctt gatcgcgttg
     1801 gtccttagct tggcctggtg gatgtatgaa tcgggccaaa aattcgccgg ttttgatcgc
     1861 ttcaaggtgg agactgacct tgccactcag accgaactca attccaagct gcatgaagaa
     1921 aacgccacgc tgcgtgacca ggttgccatt agtgagcgcc aacttcaaat tgaacgctcg
     1981 acctacggcg acctcgcgaa gcaggtaaaa gccttaagcg acgaaaacaa cagcctcaag
     2041 gaagatctcg cgttttttca gagcctgctc tctgctggca gcaagcagga ggtgagcatt
     2101 taccgcttca acgtcgagcg cgatgcgttg cctggcgaat accgctaccg tctgctgctc
     2161 ctgcagatgg ggccgcgaca gaaggagttt cagggcaagc tgcagtttat cgttgacgtg
     2221 gagcatgacg gcgaaagaag tgtcattacc atccctaatg aaaacgacgc ccatcttcag
     2281 gcgtataatg tgagtttcaa gtactatcag cgggttgaag gcagcttccg gatggagccc
     2341 ggcgccgtgg cgaaaagcgt gcaggtgcgc gtgttcgaac gcggcgtgag cgaagccaag
     2401 ctcacacaga ccgtcgacct gtcttgaaat ggagaatagc gcatgtttgg caagaaaccg
     2461 agtaaaccgc aaaatcgcat cgattgcctg atcggcgttg gcaccagcat cgaaggcaat
     2521 atcaatttca gcggcggcct gcgcgtggac ggccatgtcc gcggcaacgt ggtggcgaac
     2581 ggcgaagacc ccagcaccct ggtgttgagc gagcaagcgc gcatcgaagg cgaaatccgc
     2641 gtttcgcacg tggtcatcaa cggcaacgta atcgggccgg tacacgccgg tgagtacatt
     2701 gaattgcagt caaaatccaa tgtcaccggc gatgtgtatt acaagacgct ggaaattcag
     2761 ctcggcgcgg tggtgcaggg acggctggta tatcaggacg gcgagaatgc cgagaaagtc
     2821 ctggtgttca agcctgccac cggcgattga cccgccctgg aacgaaagag gataatacgg
     2881 gtcttaaagt aatgatgatt aggagcaggt aatgaatacc atgacggaaa tcccggcgcc
     2941 cttggtcttt accgacaacg ccgcaggcaa agtcaagcaa ctgctggaag aagaaggcaa
     3001 tcccgacctc aaactgcgcg tgtttgtgac tggcggcggc tgctcgggct tccagtacgg
     3061 gttcactttt gacgaagtag tgaacgagga tgatacctcg atgcagaaaa acggcgtgac
     3121 gctgctgatt gacccgatga gctaccaata cctggctggc gctgaaatcg actaccagga
     3181 aggcgcggaa ggcgcgcaat tcgtcatcaa gaatcccaac gctacctcca cctgcggctg
     3241 cggttcgtcc ttctcggcgt aagaattttt ttaatgagtg aacgggagcc ttaatggctc
     3301 ccgtttattt tttgtaaatc gcgccgagca cacgcgggcc tttggcgccg gttgcttgcg
     3361 gcatgtttcc cggttttccc tgcagtgttt gccaggcgag ccaggcaaac gcataagctt
     3421 cgagcgtttg cgcgttaatt cccagcgcgt cggtgacgtc ggtccttacc ctgggtagag
     3481 catcgcgcag atttttcacc agtgctttat tgcgtgcgcc gccgccgcaa aagtaaattt
     3541 cctgcgctcc ccggcagtat ttttgaatgg cctgggcgat accttgcacc gtgagcgcga
     3601 gcagtgtggc ctgcacgtct tgcggcgcct cttttcctga caggaatccg ttcagccact
     3661 gcagattgaa ttgctcgcgt ccgccgcttt tgggggggga ctcgacaaaa aagggatgaa
     3721 taagcaagcg ctgcagcagt tctggaataa tttgtccgct catcgcccaa tcaccgtccg
     3781 catcgtagcg ctgaccggtg tgcttttggc accaggcgtc gagcaacatg ttgcccggac
     3841 cgcagtcgaa accggaagcc gggctttcag gcggaagatt ggtgagattg gccatgccgc
     3901 cgatattaac gatgacgcga tgagcattcg agcccaagct gagcgccgcg tgaaacgccg
     3961 gcaccagcgg cgcgccctgg cctgcggcgg cgatatcgcg gctgcgaaaa tcgcagacca
     4021 cggtaatctc ggtaagctcc gccagcagcg ccccattgca aagctgcaag ctataaccca
     4081 gttgcggcgc atgacgcacc gtctggccat gacagccgat ggcagtaatc tcttgcgcct
     4141 ttactccggc ttgtgtaagc aactcggcaa ttgccgccgc atagcaacgc gtcaattcgt
     4201 tggcggcgag catggtttca tgcaattcgt tggcctgtgg ccgatgcaac aggagcaggc
     4261 ggctacgcaa atcgtcggga tacgagcgat agacatgctc tctgagttgc cagggtgtgt
     4321 tgccgaaatc ggcgaggatg gcgtcgatgc cgtccaggct ggtaccggac atgagtccgg
     4381 cataaagttg ccgggtgctc aagagcgaag atgggaggac aaattaa
//
LOCUS       DATIOW010000126        15113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_161516, whole genome shotgun sequence.
ACCESSION   DATIOW010000126 DATIOW010000000
VERSION     DATIOW010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15113
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161516"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1540
                     /locus_tag="VLL03_04125"
     CDS             <1..1540
                     /locus_tag="VLL03_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HSS46586.1"
                     /translation="PAAILESFLLLQQHPELKGMSAATLRALWRAKKRMNPAFRRNAK
                     NRALFMQILRQPSGITRELRRMNQYGLLGQYLPAFGRIAGQMQHDLFHVYTVDEHILK
                     VVRNLRRFTLMEFAHEYPLCSRLMSEFPRQEVLILAGLFHDIAKGRGGNHSELGSADA
                     RRFCKQHELSVEDTALVAWLVENHLMMSVTAQKQDLSDPEVIDAFARRVGNERRLIAL
                     YLLTVADIRGTSPKVWNAWKGKLLEDLFWLARKRLSGEAVSRDSFLQARQTEALDIVR
                     LYALREDAHQSLWSQLDTVYFLRHDAQEIAWHTRQLWSRVNAQAPLVKARLSPIGEGL
                     QVLIYTLDQKDLFARICGFFDHISYNIVEAKIHTTRHHYALDSFLVLDPSNKTAHYRD
                     LIGFVEHQLAQRIATQAPLEPPAKGRQSRHVKHFPIAPEVVIRPDERGAHHILSVTAA
                     DRPGLLYSIARVLVNYHINMHAAKINTLGERAEDVFLIDGEALNNSKKVVRLETDLLR
                     TLAA"
     gene            complement(1543..2076)
                     /gene="def"
                     /locus_tag="VLL03_04130"
     CDS             complement(1543..2076)
                     /gene="def"
                     /locus_tag="VLL03_04130"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HSS46587.1"
                     /translation="MAVKAVLKMGDPRLLEVAKQVEKFDTPELHQLIADMGDTMEALN
                     GAGLAAPQIGVGLQIAIFGVTRNPRYPDAEEVPYTVLINPGLMPLGEEMEEGWEGCLS
                     VPGMRGLVPRYRRLRYQGRDQFGKAIDRSVEGFHARVVQHEYDHLQGILYPMRIRDLR
                     NFGFNEILFPGQLLPDE"
     gene            complement(2066..2812)
                     /locus_tag="VLL03_04135"
     CDS             complement(2066..2812)
                     /locus_tag="VLL03_04135"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HSS46588.1"
                     /translation="MLAIIGGTGLTKLANLETSHREVVRTPYGEPSCALTFGKLREHD
                     VIFLARHGYGHTIPPHKVNYRANIWALHTLKVTHIVSVASVGGIRADLGPGTLAIPDQ
                     VIDYTHGRAHTFFEDLDKPVTHIDFTWPYCEKLRQRFIRAAEKAKQKVVVGGVYGATQ
                     GPRLETAAEIRRMEDDGADMVGMTGMPEAALARELGLCYATIAVVANHAAGKGDSAKA
                     IQFENLEAVMQQSMSRVKIILENLVELDGG"
     gene            complement(2829..3380)
                     /locus_tag="VLL03_04140"
     CDS             complement(2829..3380)
                     /locus_tag="VLL03_04140"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HSS46589.1"
                     /translation="MKLTKDKAWDILNSADEVCSAAAVTDAIKRIAGEISAKLADKQP
                     LVLSVMGGAVVFTGHLLPQLKFPLDFDYIHVSRYDDETRGGMVNWKVAPRENVKDRVV
                     LVVDDILDEGHTMAAIRERILAAGAKSFYCAVLVEKAIGKDKPIRADFVGVTVPNRYV
                     FGCGMDAYGAWRNLPAIYALKTS"
     gene            complement(3407..4282)
                     /gene="galU"
                     /locus_tag="VLL03_04145"
     CDS             complement(3407..4282)
                     /gene="galU"
                     /locus_tag="VLL03_04145"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011656638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HSS46590.1"
                     /translation="MNKIRKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVRAGITELVFITGRGKRAIEDHFDKAYEVETELYARGKKKLLKSVQDILPKNTRCIY
                     VRQPEALGLGHAVLCAANIIGDEPFAVILADDLIDAEPSVIKQMINIYRKYQRSVLGV
                     QKVPRKDTSQYGIVKTGALAPRLHSITGIVEKPRPDKAPSTLGVVGRYILTPRIFHYL
                     KNIKPGSGGEIQLTDAIAALLSEESVLAYEFRGGRYDCGSKLGYLKATLAYAAKHPEV
                     GKEFSAYLASLGKRV"
     gene            complement(4303..6327)
                     /gene="ligA"
                     /locus_tag="VLL03_04150"
     CDS             complement(4303..6327)
                     /gene="ligA"
                     /locus_tag="VLL03_04150"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HSS46591.1"
                     /translation="MKAPAEVVDRVRALRESIERCNYEYYALAAPTVPDSEYDRLFAE
                     LVDLETKYPQLVTPDSPTQRVGTAPLKEFAQVVHRTPMLSLNNAFNDDDVAAFDRRAR
                     EGLGTEEEIEYAVEPKFDGLAVGLTYEKGTLVSGATRGDGFTGEDITANLRTIKVIPL
                     RLPARGLPALFEVRGEVLMLKRDFEKLNRLQRDKNEKEFANPRNAAAGSLRQLDSRIT
                     ASRRLAFFAYGVGRVEGGVLPRDKHSKLMDYLVQLRFPVASERKVAQGIKSLLGYYRR
                     IGEKRDSLPYDIDGVVYKINDFGLQEKLGFVARAPRFALAHKFPAQEALTEVLDISVQ
                     VGRTGALTPVARLKPVFVGGANVTNATLHNEDEVRRKDVWRGDTVVVRRAGDVIPEVV
                     SVARKGLRQDGDYFKMPKQCPICHSKVVRSEDEAVARCSGGLFCPAQRKQALLHFASR
                     RAMDIEGLGEKLVDQLVDNQIVKTPADLYKLRLTALANLERMAEKSAGNVLAAIAKSR
                     RTTLPRFIYALGIRNVGEATAKDLARHFGSLDKLMQANAETLLQVQDVGPVVAQSIAQ
                     FFAERHNREVIKQLRGCGVTWSEDKGAPTTTAGAVNGKTFVLTGTLPRLMREEAKERI
                     EALGGKVSGSVSGKTDYVIAGADPGGKLAKAQELGVKILDEAGLRKLLGE"
     gene            complement(6328..7398)
                     /locus_tag="VLL03_04155"
     CDS             complement(6328..7398)
                     /locus_tag="VLL03_04155"
                     /inference="COORDINATES: protein motif:HMM:NF016257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HSS46592.1"
                     /translation="MSDLQLSLLAIGILIVAATYAFNWLQERKYRRKSESAFKNQFHD
                     VLLESRDTPGEERVEPRLGEVDTQEAPPASRPVFQSEAANAALDPEIALIAEVSRAQR
                     FSAEETKMLAKRLLEFGRRAVVCSYNEMNRCWEKLAETDSVRCSRVKVGLQLADRSGC
                     VKESRLALFYDLLQGFAETTAADLLLPDRNAALEQAVELDQFCAEVDISIGLNVIAQD
                     GPAFPGTKLRALSEAAGLKLLGDGAYHCLNEQGESLYALSNHEAAPFTSEQIKNLSIP
                     GVTLLLDVPRVASGLRVFDQMLAFARQLAAALGGKLVDDNGAPLNDAGVEKIREQLRA
                     IYMLMERRNISAGSPRALRLFS"
     gene            complement(7408..10923)
                     /gene="smc"
                     /locus_tag="VLL03_04160"
     CDS             complement(7408..10923)
                     /gene="smc"
                     /locus_tag="VLL03_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004527298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HSS46593.1"
                     /translation="MRLTQIKLAGFKSFVDPTYIPIPGQLVGIVGPNGCGKSNIIDAV
                     RWVLGESQARHLRGETMQDVIFNGSGERAPVSRASVELIFDNSRGQAAGQWSIYAEIS
                     IKRVLTRNGESSYYINNTHVRRRDVTDIFLGTGLGPRAYAIIEQGMISRVIEARPEEL
                     RVFLEEAAGASKYRERRKETELRLKDTRENLLRIADVRQELEKQLQHLEEQARLAVQY
                     RELQNQLHTSQNLLWLIKKQDATASRNRVEKEIQKLGNELEADTAKLREVENRLEQVR
                     TRHYSAGDAVHQAQGELYSANSEVSRLEQQIQHIRENRQRIESQLASLRQQLEQLEDH
                     KQSTRQTLMQLQSERVRLQQLIEEAERKAGAEARLLPEVEQAFRNCQERVLGLQRELA
                     QAQQSAQIEETHRAHAQKMLQQLEIRRLRLLEERETLPKTDQTELQRLKLEQAQAAQA
                     LKEKQLKLDSLQQRQPELEQKKEALNHKLQTREQALAGFMARLDTLKQLQLRLQTDGK
                     LQPWLAKHHLEEVRRLWRELRIEQGWENALESVLRERLSALGLNKTAGLQDLLNDAPP
                     VKLTVFVLPQQGASVAMVGDLKPLLNLVTFNNPGIRALLEDWLHNVYCADDEPLKQRE
                     LLPPGALLVSREGHIFTRNSVSFHAADSEMHGVLARSREIEQLEAEAGSQRSLLEEQR
                     AALEGVQQQWQSLQAETAALRSETEALQAQLHQAQIAALKFTQNAEQVQRRAEQIAAE
                     LEEICPQLEHETQQQQTAGDNFASSRSQVAALQEHLHDAGAEHGAAETRLNEQREQLN
                     QAQRSLHESRFLERENINRINEINNSIKVLDEQACGLSKTLEQGNVEHRNLDETLYQE
                     QLQRALQAKQLKEQALAAARNTLEEFTTGLQQTEEERLTTERQLEPLRNRIHEMQLKE
                     QEARLNEEQHAEQLRTAAADEEQLAALLRKGTKAAALQVEIARLNEEITALGAVNLAA
                     LEELQSAKERKNYLDNQVKDLDEAVETLGNAIRRIDRETRARLLETFEAVNRHLSEMF
                     PMLFGGGQARLVLSGEEILDSGVQIIAQPPGKKTSSIHLLSGGEKALVALALVFSLFQ
                     LNPSPFCLLDEVDAPLDDTNTESFCNLVRKMSEQIQFLFISHNKITMEIAHQLIGITM
                     PEQGVSRVVAVDIEEAMKLREEAVA"
     gene            11002..11418
                     /gene="queF"
                     /locus_tag="VLL03_04165"
     CDS             11002..11418
                     /gene="queF"
                     /locus_tag="VLL03_04165"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HSS46594.1"
                     /translation="MPTQLAKNLETFLNPKAGRDYLIHMEIPEFTCLCPKTGQPDFAK
                     LALDYIPDKKCVELKSLKLYIWSFRNEGAFHEAVTNRILDDLVNAVKPRYMRLTARFY
                     VRGGIFTSVIAEHRKSGWEPPVQVDLQPFEEPSNTR"
     gene            complement(11422..12309)
                     /locus_tag="VLL03_04170"
     CDS             complement(11422..12309)
                     /locus_tag="VLL03_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSS46595.1"
                     /translation="MFTKQKMVPVSALLTGATVWGLIWYPYRILQEAGMAGDIATFIT
                     YSLALLAGLIVYRGAFKEIGRAPWIVLWIGLSAGWTNLAYVLAVIEGEVVRVLLLFYL
                     APLWTVILAGTLLEERINAYGLLVVILSLAGAVTMLWHLESGWPLPQNSAEWLGLSAG
                     FMFAVSNVLARKAHGISLQLKALSIWAGVALFGLVVAAGTGHGLPSRLGGYDGLLLLL
                     VALVIFLVNPVVQYGLSHVSANRAIVIFMFELVVGAVSAYVLADEALSLQDAIGGAMI
                     IAASLLSGRLELESRENPA"
     gene            12371..13567
                     /gene="dapC"
                     /locus_tag="VLL03_04175"
     CDS             12371..13567
                     /gene="dapC"
                     /locus_tag="VLL03_04175"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HSS46596.1"
                     /translation="MNSYLDRLQPYPFQKLAQLFEGITPNPDLAPINLSIGEPKHSAP
                     EFIKKALADKLNGLANYPITRGSFNLREAIAAWLKRRFQLREISAENEILPVNGSREA
                     LFAFAQAVVDSGAGQPMVVCPNPFYQIYEGAALLAGATPQFLNLLPQHHYALHFKELD
                     ESIWRQTQLIYVCSPGNPSGSVMTLEEWEKLFKLSDQYGFTIASDECYSELYLDESNP
                     PLGGLEAAQKLGRERFPRQVAFGSLSKRSSVPGMRSGFAAGDAQIIKKFHLYRTYHGC
                     AMSPPVQAASIAAWNDEAHVVENRRLYREKFARVTPLMKKWMPGLETPQGGFYLWAQT
                     PLPDTEFAWLLYRDYNVALLPGSYLAREAHGINPGKNFVRVALVAPLAECVEAAERIA
                     EFIEQL"
     gene            13573..14394
                     /gene="dapD"
                     /locus_tag="VLL03_04180"
     CDS             13573..14394
                     /gene="dapD"
                     /locus_tag="VLL03_04180"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HSS46597.1"
                     /translation="MKQLQTIIDEAFERRAQITPQNAGARLKKTVTQAVELLDAGKLR
                     VAEKINGRWVTHQWVKKAVLLSFRINDNIAVKNGFTNYYDKVASKFAGYGPKAFREGG
                     FRVVPPATARKGAYIAKNVVLMPSYINIGAYVDEGTMVDTWVTVGSCAQIGKNVHLSG
                     GVGIGGVLEPVQASPTIIEDNVFVGARSEVVEGVIVEEGAVISMGVFIGQSTKIFNRE
                     TGEITYGRVPSGSVVVSGNLPSADGKYGLYCAVIVKQVDAKTRAKVGINELLRGA"
     gene            14604..>15113
                     /locus_tag="VLL03_04185"
     CDS             14604..>15113
                     /locus_tag="VLL03_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46598.1"
                     /translation="MKFLDELKKEAEERKQQEALETQSKLSALGQNFMQLQAKLRQIY
                     AYLDELVKQLNVVKPVVLRSYYLEGLGNFEGLNQKEYALNKTHKSIDNKDFIDTITLR
                     LKCTAEGALSTEKDTPGLTKTMCEYLWDFKIKFELQEMKNQRGLVDRAVFKVPREVPV
                     TVVFNGNLEK"
BASE COUNT         3160 a         4485 c         4074 g         3394 t
ORIGIN      
        1 gccagcggca attctggaaa gcttcctgct gttgcagcag cacccggagc tcaagggcat
       61 gagcgcggcc accttgcgcg ccttgtggcg ggcaaaaaaa cggatgaatc cggctttccg
      121 gcgcaacgcg aaaaaccgcg ccctgttcat gcagatcctg cgccagcctt ccggcatcac
      181 gcgcgaactg cgccgcatga accagtacgg gttactcggc caatacctgc cggcattcgg
      241 ccgcatcgcc gggcagatgc agcatgatct gttccatgtc tacactgtgg acgagcatat
      301 tctcaaggtg gtgcgcaacc tgcgccgctt taccttgatg gaattcgccc atgaatatcc
      361 tctttgcagc cggttgatga gcgaatttcc gcgccaggag gtgttgatcc tggcaggatt
      421 gtttcacgat atcgccaaag ggcgcggcgg caatcactcc gaactgggca gcgccgacgc
      481 gcgtcgcttc tgcaagcagc atgagttgag cgtcgaggat acggctttgg tcgcctggct
      541 ggtggaaaat cacctgatga tgtctgtcac cgcgcaaaag caggatttgt ccgacccgga
      601 agtaatcgat gccttcgccc ggcgcgtggg caatgagcgc aggctgatcg cgctatacct
      661 tctcacggta gccgacatcc gcggcaccag ccctaaggtg tggaacgctt ggaaaggaaa
      721 actgctcgaa gacctgttct ggctcgcccg caagcgcttg agcggcgaag cggtgtcacg
      781 cgacagtttc ctgcaggcgc ggcaaaccga ggcgctcgac atcgttcgcc tgtacgcgct
      841 gcgcgaagac gcgcaccagt cgctatggtc acagctcgac accgtttatt tcctccgtca
      901 cgatgcccag gaaatcgcct ggcatactcg ccagctctgg tcgcgggtga atgcgcaagc
      961 gcctttggtc aaggcgcggc tctcccctat cggcgaaggc ttgcaggtgc tgatttatac
     1021 gctggatcag aaagacttgt ttgcgcgcat ctgcggtttt tttgaccaca tcagctacaa
     1081 catcgtcgag gccaaaattc acaccacgcg ccaccattac gcgctggaca gtttcctggt
     1141 gctcgatcct tccaacaaaa ccgcgcacta ccgcgacttg atcggttttg tcgaacacca
     1201 gctggcgcaa cgcattgcga cgcaagcgcc gctcgaaccg ccggccaaag gacgccaaag
     1261 ccgtcatgtc aagcatttcc cgattgcgcc agaagtcgta atccgccccg atgagcgcgg
     1321 cgcgcatcat attctttccg tcaccgccgc cgaccgaccg gggctgctct acagcatcgc
     1381 acgggtgctg gtgaactacc acattaacat gcacgcggcg aaaatcaata cgctgggtga
     1441 gcgcgccgaa gacgtgttct tgatcgacgg tgaagcgttg aacaacagca agaaagtggt
     1501 gcggctggaa accgatttgc tgcggacgct ggccgcctaa acctactcgt ccggcaacaa
     1561 ctggccagga aacaagattt cgttgaagcc gaaattgcgc aggtcgcgga tgcgcatcgg
     1621 ataaaggatt ccctgcaaat ggtcgtattc gtgctgcacc acgcgcgcat gaaaaccttc
     1681 gacgctgcgg tcgattgcct tgccgaattg gtcgcggcct tgataacgca gccgtcggta
     1741 gcgcggtacc agcccgcgca tgcccggtac gcttaagcag ccttcccagc cttcttccat
     1801 ttcttcgccc agcggcatga gcccgggatt aatcaggacg gtataaggaa cttcctcggc
     1861 atcggggtag cgcggattgc gggtcacgcc aaaaatggca atttgcaaac ccacaccgat
     1921 ttgcggcgcg gcgagcccgg cgccattcag cgcttccatg gtgtcgccca tgtcggcaat
     1981 cagctggtgc aactccgggg tgtcaaattt ttccacctgc ttcgctactt ccagaagccg
     2041 cgggtcgccc attttcaaaa ctgctttaac cgccatcgag ctcgaccagg ttttccagaa
     2101 taatctttac gcggctcatc gattgctgca tcaccgcttc caggttttca aattgaatgg
     2161 ccttggcgct gtcgcccttg ccggccgcgt gattggccac tacagctatg gtcgcatagc
     2221 acagcccaag ttcccgggcc aaagccgctt ccggcattcc ggtcatgccc accatgtcgg
     2281 cgccgtcatc ttccatgcgc cggatttccg ccgcggtttc cagtcgcggc ccctgggttg
     2341 caccatacac gccgcccacg accactttct gtttggcttt ttccgcggca cggataaagc
     2401 gttgacgcag cttctcgcag tacggccatg tgaaatcgat atgggtaacc ggcttgtcca
     2461 ggtcctcaaa aaaagtatgc gcgcgcccgt gggtgtaatc gatgacttga tcgggaatgg
     2521 caagggtccc cggcccgagg tcggcgcgta tcccacccac cgaagccacc gacacgatat
     2581 gcgtgacttt tagcgtatgc aacgcccaga tattggcgcg gtaattcacc ttgtgcggcg
     2641 gaatggtatg accgtagccg tggcgcgcga ggaaaattac gtcatgttca cgcagcttgc
     2701 cgaaagtcag tgcgcaggaa ggctcaccgt aaggcgtgcg tacgacttcg cggtgcgacg
     2761 tttccaggtt ggcgagcttg gtgagccccg tgccgccgat aatcgccagc atgcttttct
     2821 ccgcttcttt aactggtttt gagtgcataa atcgcgggca aattgcgcca ggcaccgtac
     2881 gcatccatgc cgcagccgaa gacatagcga ttgggaacgg tgacccccac aaaatccgcg
     2941 cgtattggtt tgtccttgcc gattgccttt tccaccagca cggcgcagta aaaagacttc
     3001 gcgccggcgg caaggatgcg ctcgcgtatc gccgccatgg tatgcccttc atcgagaata
     3061 tcgtccacaa ccagcaccac tctgtctttc acattttccc gcggcgcgac tttccagttg
     3121 accatgcccc cccgtgtctc gtcgtcatag cgcgaaacat ggatgtaatc aaaatccagc
     3181 gggaacttca gctgtggcag taaatggccg gtaaatacca ccgcgccgcc catgacgctc
     3241 aataccaaag gctgtttgtc ggcaagtttt gcgctgattt caccggcgat acgttttatt
     3301 gcatctgtaa cggcagcggc ggaacagact tcatccgcag aatttaggat atcccaggct
     3361 ttatctttgg taagcttcaa tgttgttttt aaaaattttg actggattat acccgcttgc
     3421 caagcgaggc aagataggcg ctgaattcct tccctacttc cgggtgcttg gccgcgtaag
     3481 ccagtgtagc tttgagatag ccgagtttcg agccgcaatc gtagcgcccg cccctgaatt
     3541 cataggcgag caccgattct tcgctgagca gtgcggcaat agcgtcggtg agctggatct
     3601 cccctcccga tcccggcttg atgtttttca aatagtgaaa aattcgcggc gtgagaatgt
     3661 aacgaccgac cacccccagg gtggaaggcg ccttgtccgg tcttggtttt tccacaatgc
     3721 cagtaatact atgaagcctc ggggccaagg caccggtttt gacgatgccg tattgcgagg
     3781 tgtcttttct cggcaccttc tgcaccccca gcaccgagcg ttgatatttg cggtaaatat
     3841 tgatcatttg ctttatcacg gatggttcgg catcaatcag gtcgtcggcg agaattaccg
     3901 caaacggctc atcgccgatg atgttggcgg cgcacaatac ggcatgtccc agccccagtg
     3961 cctccggctg tcgcacataa atacatctgg tgttcttggg aagaatgtcc tgaactgact
     4021 tgagcagctt ttttttgccg cgggcgtaca gctcggtttc gacttcataa gctttgtcaa
     4081 aatggtcttc aatcgcgcgt ttgcccctgc cggtgatgaa caccagctcg gtgattccgg
     4141 cgcgcaccgc ttcttccaca gcgtactgga ttaaaggttt gtcaacgatg ggcagcattt
     4201 ccttggggct ggccttggtt gccggcaaaa agcgggtacc catgccggcg acgggaaata
     4261 cggctttgcg gattttgttc atgagtctga agttaatgag attcactcgc ccagaagctt
     4321 gcgcaggccc gcttcatcga gaattttcac tcctaattcc tgcgccttgg ccagcttgcc
     4381 gcccgggtcg gcgccggcaa tcacataatc ggttttgccg gagacgctgc cgctgacttt
     4441 gccgcccagc gcttcgattc tctccttcgc ctcctcgcgc atcagacgcg gcagggtgcc
     4501 ggtgagcaca aaggtcttgc cgttcaccgc gcccgcggtt gttgtcggcg cgcctttgtc
     4561 ttcagaccaa gtcacgccgc agccgcgcag ttgtttaatc acttcacggt tgtgccgttc
     4621 ggcaaaaaac tgcgcgatgc tttgcgccac gaccgggccg acatcctgca cttgcagcaa
     4681 ggtctccgca ttggcctgca tcagcttgtc gagactgccg aagtgccgcg ccaggtcttt
     4741 cgccgtcgct tctcccacat tgcggatgcc gagcgcgtaa atgaagcgcg gtagcgtggt
     4801 acgcctgctc ttcgcaatcg ccgccagcac attaccggcg gatttttccg ccatgcgctc
     4861 cagattggca agcgccgtga gccttagctt atataaatcc gccggtgttt tcacgatctg
     4921 attgtccacc agctggtcaa ccagcttctc gcccagccct tcgatatcca tcgctcggcg
     4981 cgaggcgaag tgcaaaaggg cctgcttgcg ttgcgcaggg cagaacaacc cgccgctgca
     5041 acgcgccact gcttcatcct cggaacgcac cactttggaa tggcatatgg ggcattgttt
     5101 cggcatcttg aaataatctc cgtcctgccg caggcctttg cgcgccacac ttaccacctc
     5161 gggaatgaca tcgcccgcgc ggcgcaccac cactgtgtcg ccgcgccaga cgtccttgcg
     5221 cctcacttcg tcttcgttgt gcagagtggc attggtgacg ttggctccgc cgacaaacac
     5281 tggcttgagc cgtgcgacgg gggtaagtgc accggtccgc ccgacctgta ctgagatatc
     5341 cagcacttcg gtcagcgctt cctgagcggg gaacttgtgg gccaaagcaa agcgcggcgc
     5401 gcgagccaca aacccgagct tttcctgcag accgaaatca ttgattttgt acaccacgcc
     5461 gtctatgtca taaggcaggc tgtcgcgttt ttcgccgatg cggcggtaat aaccgagcaa
     5521 gctttttatc ccctgcgcca ccttgcgttc agacgccacc ggaaagcgca gttgtaccag
     5581 ataatccatc agcttgctgt gtttgtcgcg cggcaacaca cctccttcca cccggccaac
     5641 gccataggcg aagaatgcca gcctgcggct ggcggtgatg cgcgaatcca gttgccgcag
     5701 cgatccggcg gcggcattgc gcggattggc gaattccttt tcgtttttat cccgctgcaa
     5761 ccggttgagc ttttcgaaat cccgcttcag cattaggact tccccgcgca cctcaaacaa
     5821 ggccggtagg ccgcgcgcgg gaagccgcag cggaatgacc ttgatggtgc gcaagttggc
     5881 ggtgatatct tcgccggtaa agccgtcgcc gcgggtggcg ccgcttacca gcgttccttt
     5941 ttcataggtc agccccactg ccaggccgtc aaatttgggc tcgacagcgt attcgatttc
     6001 ctcttccgtt cccagaccct cgcgcgcccg gcggtcaaat gccgccacgt catcatcgtt
     6061 aaacgcgttg ttgagcgaca gcatcggcgt gcgatgaacg acttgcgcga actccttgag
     6121 cggggccgta cccacgcgct gcgtgggcga atcgggagtc accagctgcg ggtacttcgt
     6181 ctccagatca accagctcgg caaatagcct gtcgtattcg ctgtcgggaa cagtcggcgc
     6241 cgcgagcgca tagtattcgt agttgcagcg ctcgatgctt tcgcgcaagg cgcgaacccg
     6301 gtcgacaacc tccgccggcg ctttcattca ggaaaaaagc cttaaggcgc gaggactgcc
     6361 cgcactgata ttgcgtcgct ccatgagcat gtagatggcc cgcagctgct cacgaatttt
     6421 ttccaccccc gcatcattaa gcggggcccc gttgtcgtcc accagcttcc cgcccaacgc
     6481 ggccgcgagc tgcctggcaa aggcgagcat ctggtcaaaa acgcgcaacc cggaagccac
     6541 tctcggtaca tccaggagta aggtcactcc gggaatggaa aggttcttga tttgctccga
     6601 cgtgaacggc gccgcctcat gattggaaag cgcgtacaaa ctttctccct gctcattgag
     6661 gcaatggtaa gcgccgtcac ccagcaattt aagcccggcg gcttcactca gcgcgcgcaa
     6721 cttggttccc ggaaaagccg gtccgtcttg ggcgatgacg ttaagtccta tcgaaatatc
     6781 cacttcggcg caaaactggt cgagctccac ggcttgttcc agcgctgcat tgcgatcggg
     6841 aagcaagagg tcggcagcgg ttgtctcagc aaagccttgg agcagatcat agaacagggc
     6901 aagccgcgat tccttgacgc aacccgagcg gtcggcaagc tgcaggccga cttttacccg
     6961 ggaacaacgt acagaatcgg tttcagccag tttctcccag caccggttca tttcgttata
     7021 gctacaaacc actgcccggc gcccgaactc gagcaaacgt tttgccagca ttttcgtctc
     7081 ttcagcggaa aaccgctggg cgcggcttac ttcggcaatt aaagcaatct ccggatcgag
     7141 tgcggcgttc gcagcttccg attgaaacac aggcctggat gctggcggtg cttcctgagt
     7201 atcaacttct cccagtcgcg gctccactct ttcctcaccg ggcgtatccc gggattccag
     7261 caggacgtcg tgaaattggt ttttgaaagc ggattctgac ttgcgccgat attttctctc
     7321 ctgcagccaa ttaaatgcat aagtcgcggc aacgatgagg atgccgattg ccagcaagct
     7381 aagttgcaag tcgctcataa tgagcctcta tgcgacggct tcttccctca gtttcatcgc
     7441 ttcttcgatg tccaccgcca ccacgcgcga caccccctgc tcgggcatgg taatgccgat
     7501 gagctggtgc gcgatttcca tggtgatttt attgtggctg ataaacaaaa actgaatctg
     7561 ttcggacatt tttctgacca ggttgcagaa actttcagta ttggtatcgt cgagcggcgc
     7621 gtccacctca tccagcaggc aaaacggcga cggattaagt tggaacaacg agaacaccag
     7681 agccagggct accagcgctt tctccccgcc agaaagtaga tgaatcgaac tggttttctt
     7741 gcctgggggc tgggcgataa tttgcacccc gctgtcgaga atttcctcac cgctcagcac
     7801 cagcctggcc tggccgccgc caaataacat cgggaacatt tcgctcaaat ggcggtttac
     7861 cgcctcaaag gtttccagca ggcgggcgcg ggtttcgcgg tcgatgcggc ggatggcgtt
     7921 tcccaacgtt tccaccgctt catccagatc cttgacctgg ttgtcgagat agtttttgcg
     7981 ctcttttgcg ctttgcagtt cttccagcgc cgcgagattg accgccccca gcgcggtaat
     8041 ttcttcgttc aagcgggcga tttccacctg cagtgcggca gctttcgttc ctttccgcaa
     8101 aagtgctgcc agttgttctt cgtcggcggc ggcagtgcgc aattgctcgg cgtgctgctc
     8161 ttcattcaag cgtgcttcct gctctttgag ctgcatttca tggattcggt tgcgcagcgg
     8221 ctcgagctgg cgctcggtgg tgagccgctc ttcctcagtc tgctgcaagc cggtagtgaa
     8281 ttcctccaac gtgttgcgcg ccgccgccaa ggcctgttcc ttgagctgct ttgcctgtaa
     8341 agccctttgc agttgttcct ggtaaagggt ttcgtccaga ttcctatgct ccacgttacc
     8401 ctgctccaga gtcttgctca agccacatgc ttgctcatct aacaccttaa ttgaattatt
     8461 tatttcattg attctattta tattttctct ttctaaaaat cttgattcat ggagtgaccg
     8521 ctgggcctga ttgagctgtt cgcgctgttc atttaatcgg gtttcagcgg cgccatgttc
     8581 tgcgccggca tcgtgcaaat gctcttgtag cgccgcaact tgactgcggg aggaagcaaa
     8641 attgtccccg gcagtttgct gctgctgggt ttcatgctcc agttgcgggc agatttcttc
     8701 gagttcggcg gcaatctgct ccgcgcggcg ctgcacctgt tcggcgtttt gggtgaattt
     8761 cagcgccgcg atttgcgctt ggtggagttg agcctgcaaa gcttcggttt cgctccttaa
     8821 tgcggccgtt tccgcttgca gggattgcca ttgctgttgc acgccttcca gtgcagcgcg
     8881 ttgttcttcc agcagcgaac gttggcttcc cgcttccgcc tccagttgct cgatttcacg
     8941 gctgcgggca agcacgccgt gcatttccga gtctgcagca tggaacgata cactgttgcg
     9001 ggtaaagata tgcccttcac gactgaccag aagcgccccc ggcggcaaca gctcacgctg
     9061 tttaagcggc tcatcatccg cgcaatagac gttatgcagc cagtcctcga gcaaggcacg
     9121 gatgccggga ttattgaaag tcaccagatt cagaagcggc tttaggtcgc ccaccatcgc
     9181 aaccgatgcg ccctgctgcg gcaagacaaa cacggtaagc ttcaccggcg gcgcatcgtt
     9241 caacaaatcc tggagcccgg cggttttgtt caagcccaac gcgctcagcc gctcgcgcag
     9301 cacgctttcc agcgcatttt cccagccttg ttcaatccgc agctcccgcc acagcctgcg
     9361 cacttcctcg agatggtgtt tggcgagcca gggctgcagc ttgccatcgg tttgcagcct
     9421 cagctgcaac tgcttcaagg tatcgagccg ggccataaag ccggccagcg cctgctcacg
     9481 ggtctgcagc ttgtggttga gcgcctcctt tttttgctcc aattcaggct ggcgctgctg
     9541 cagcgagtcc agttttagtt gtttctcctt gagcgcttgt gcggcctgcg cttgttctag
     9601 ctttaggcgc tgcaactcgg tttggtccgt tttggggagc gtttcgcgct cttcaagcaa
     9661 acgtaaccgg cgtatctcga gctgctgcag cattttttgc gcgtgggctc gatgagtttc
     9721 ctcgatttgc gccgactgtt gtgcttgggc aagctcgcgt tgcaatccca aaacccgctc
     9781 ttggcagtta cggaaagcct gctccacttc gggcagcagt cgcgcctcgg cgccggcttt
     9841 gcgctccgcc tcttcgatca gctgttgcag ccggacccgc tcgctttgca gttgcatcaa
     9901 ggtttgccgg gtcgattgct tgtggtcttc cagttgctcc agttgctggc gcagcgaagc
     9961 aagctgggat tcgatgcgct ggcggttctc gcggatatgc tggatttgct gctcgagcct
    10021 tgacacttca ctattagcag agtaaagctc gccttgcgcc tggtgcaccg catcgcccgc
    10081 gctataatgc cgggtgcgca cctgctccaa ccggttctca acttcgcgca acttcgcggt
    10141 atccgcttcc agttcgttgc caagtttctg gatttccttc tcgacgcggt tgcggctagc
    10201 cgtcgcatcc tgttttttga tcaaccacag cagattttgc gaagtatgca gctggttttg
    10261 caattcgcga tattggacgg cgaggcgcgc ctgctcctcc aggtgctgca actgtttttc
    10321 cagctcctgc cgcacatccg cgatgcgcag cagattttcc cgcgtatcct taagccgcaa
    10381 ttcggtttcc ttgcgccgct cgcgatattt ggatgcgccc gccgcctctt ccaggaacac
    10441 cctcaactcc tcgggcctgg cttcgatgac gcgcgagatc atgccttgct cgataatggc
    10501 ataggcgcgt gggcccaaac ccgtacccag gaaaatatcg gtcacgtcgc ggcgccgcac
    10561 gtgcgtattg ttgatgtaat agctggattc gccgttacga gtgagcacac gtttgatgga
    10621 aatctcagca taaattgacc actgaccggc cgcttggccc cggctgttgt cgaaaatcag
    10681 ctcgacgctg gcgcgggaaa ccggcgcacg ctcaccggag ccgttgaaaa tcacgtcctg
    10741 catggtttcg ccgcgcaaat gccgggcttg ggattctccc aggacccaac gtaccgcgtc
    10801 gatgatgttg gatttgccgc aaccgttggg gccgacgatg cctaccagct gccccgggat
    10861 cgggatatag gtcgggtcga caaaggattt gaacccggca agtttgatct gagtaaggcg
    10921 caatttattg tagttaggtt aaagggttat aatttgccgc aagaagctgc attattttaa
    10981 ccgatttaac tagaacaggt tatgccaact caactcgcta aaaatctgga gacctttctc
    11041 aaccccaagg caggacggga ctatctgatt cacatggaaa ttcccgagtt tacctgcctt
    11101 tgtccaaaaa ccggccagcc ggattttgcc aagctcgctc tcgactacat tcccgacaag
    11161 aaatgcgtgg agctgaaaag cctcaagctc tacatctggt ctttccgcaa cgaaggcgcg
    11221 ttccacgaag ccgttaccaa ccgcattctc gatgatttgg taaacgcggt aaaaccgcgc
    11281 tacatgcgcc tcaccgccag gttttatgtc cgcggaggga ttttcaccag cgtgatcgcc
    11341 gaacaccgca aatcgggctg ggaacccccg gttcaggttg atttgcagcc gttcgaagaa
    11401 ccatcgaaca cgcgctgact ttcaggcggg attttcccgt gactccagtt ccaggcggcc
    11461 ggaaagcagg ctcgccgcga taatcatcgc gccgccgatt gcatcctgca ggctcagtgc
    11521 ttcatcggcc aacacataag cggataccgc tcccaccacc agttcgaaca tgaatatgac
    11581 tatcgcgcgg ttggcgctca cgtgactcag gccgtattgc accaccgggt taaccagaaa
    11641 tatcaccagc gccaccagca gcaacagcaa cccatcgtag ccgccgagcc gtgaaggtaa
    11701 accatgcccg gtaccggccg ccaccaccaa accgaataac gccacgccgg cccagatact
    11761 cagcgctttc agttgcaggc tgatgccgtg cgccttgcgg gcaagcacat tggacacggc
    11821 gaacataaag ccggcggata atcccagcca ttccgcagaa ttttgcggca aaggccagcc
    11881 gctctccaga tgccacaaca tggtgaccgc acctgctaac gaaagaatca ccaccagcaa
    11941 gccgtaggca ttgatcctct cctctagcag cgttcccgcc aaaatcaccg tccatagcgg
    12001 cgcgaggtaa aaaagcagca gcactcgcac cacctcgcct tcaatcaccg ccagcacgta
    12061 ggcgagattg gtccaacccg cgctcaagcc tatccacaac acgatccatg gagcgcggcc
    12121 aatctccttg aaagctccgc ggtaaacaat gagccccgcc agcagtgcca aggaatatgt
    12181 gataaaagtg gcgatatcac cggccatccc cgcttcttgc aaaatccggt agggatacca
    12241 gataaggccc cagactgtgg cgccggttaa aagcgcggaa accggcacca ttttttgttt
    12301 tgtgaacaag caccacgccc ttatataatg cgtcgttccg gcaaacttgc tgcggaacac
    12361 gctccaagtc atgaactcat atcttgatcg cctgcaaccc tacccgttcc agaaactggc
    12421 gcagctgttt gaaggcatca cgcccaatcc ggatctggcg ccgatcaatc tttcgattgg
    12481 cgagccaaag cattctgcgc ccgagtttat caaaaaggcg ctggcggaca aactcaatgg
    12541 gttggcgaat tatcccataa cccggggaag cttcaattta cgcgaagcga ttgccgcctg
    12601 gctgaaacgc cgtttccagt tgcgcgaaat cagcgcggaa aatgaaatcc tgccggtaaa
    12661 cggcagccgc gaagccctgt tcgctttcgc tcaagccgtg gtcgacagtg gcgccgggca
    12721 accgatggtg gtgtgcccca acccgtttta ccagatttat gagggagcgg cgcttctcgc
    12781 cggcgcgacg ccgcaatttc tgaatttgct gccgcagcat cattacgctc ttcactttaa
    12841 agaactcgat gaatcgattt ggagacagac acaactgatt tatgtgtgct cgccgggcaa
    12901 cccgagcggc agcgtgatga cgctggaaga atgggaaaaa ttgttcaagc tttccgatca
    12961 atatggattc accattgctt ccgatgaatg ctattccgaa ctctatcttg acgaaagcaa
    13021 tccgccgttg ggaggactgg aggcggcgca aaaactggga agagagaggt ttccacgcca
    13081 ggtggcattc ggcagcctgt cgaaacgctc cagcgtgccc ggcatgcgct ccggttttgc
    13141 cgccggcgat gcgcaaatca tcaagaaatt tcacctctac cgcacttatc acggctgcgc
    13201 catgagcccg ccagtacaag ccgccagcat cgccgcctgg aacgacgaag cacatgtggt
    13261 ggaaaaccgg cggctctatc gtgaaaagtt tgcccgcgtc acgccgctca tgaaaaagtg
    13321 gatgccggga ctcgaaacgc cgcagggcgg tttttatttg tgggcgcaga cgcctctgcc
    13381 cgataccgag ttcgcgtggc tgctgtatcg cgactataat gtcgccttgc tgccgggaag
    13441 ctatctcgcg cgcgaagcgc atggcattaa cccgggcaaa aattttgtgc gcgtcgcgct
    13501 ggttgcgcct ctcgccgaat gcgtggaagc ggcggaacgc atcgccgaat tcattgaaca
    13561 actttaaatg ccatgaaaca actgcaaacc atcattgacg aggcctttga gcgacgcgcc
    13621 caaatcaccc cgcagaatgc cggggccaga ctcaaaaaaa ccgtcacgca agccgtcgaa
    13681 ctcctggatg cgggcaaact tcgcgtcgcg gaaaaaatca atggccggtg ggtgacccat
    13741 caatgggtga aaaaagccgt gttgctctcc ttccgtatca acgacaatat cgcagtaaaa
    13801 aacgggttca cgaattatta cgacaaagtg gcaagcaaat ttgcaggtta cggccccaaa
    13861 gcgtttcgtg aaggcgggtt tcgcgtcgtg ccccctgcca ctgcacgcaa aggcgcctat
    13921 atcgcgaaaa atgtcgtgct catgccctct tacatcaaca ttggcgctta cgtggatgaa
    13981 ggcaccatgg tggacacctg ggttaccgtg ggttcatgcg cgcaaattgg caaaaatgta
    14041 catctttcgg gaggggtcgg cattggcggc gtgctggagc cggtgcaggc cagcccgacg
    14101 attatagaag acaatgtatt cgtcggcgcc cgctccgagg tcgtggaagg cgtcatcgtc
    14161 gaggaaggcg cggtgatttc aatgggtgtg ttcatcggcc aaagcaccaa aatttttaat
    14221 cgcgagaccg gtgaaatcac ttacggccgc gtcccctccg gctcagtggt ggtttccgga
    14281 aacctgcctt cggccgacgg caaatacggt ctgtattgcg ccgtcatcgt caaacaagta
    14341 gatgccaaga cccgcgccaa agtggggatt aacgaacttt tgcgcggcgc ctagtctctg
    14401 tagcggcagg gtttttgctt gaatatcctg gaaatgggcc acaacccttt aatttctgtc
    14461 cgattattgg caaaatggac aggtcagtcg aataacctgt gcctggaggc tgcaataccc
    14521 acaaaaacga ctatactgag tacgatcaaa aagttaagcc cttgggctac cgacaatgcg
    14581 gactgggcat cgaggagtgt gcaatgaagt tccttgacga actaaaaaag gaagccgaag
    14641 aacgaaagca gcaggaggcg ctggaaaccc aatccaagct ctctgcgctc ggccagaatt
    14701 tcatgcagct acaggccaag ctgcgtcaga tttacgctta ccttgacgaa ctcgttaaac
    14761 aactcaacgt agtcaagccc gttgtcttgc gctcttatta cctcgagggg ctgggcaatt
    14821 tcgagggcct caaccagaag gaatacgcgc tcaacaaaac tcacaaaagc atcgacaaca
    14881 aggattttat cgacactatc accttgcgcc tcaaatgcac cgccgaaggc gcgctgtcaa
    14941 cggaaaagga cacgccgggg ttgaccaaaa cgatgtgcga gtacttgtgg gactttaaaa
    15001 tcaagtttga actgcaggaa atgaaaaatc agcgcggcct cgtcgaccgc gcggtattca
    15061 aggtgccccg tgaagtgccg gtgacggttg tcttcaacgg caaccttgaa aag
//
LOCUS       DATIOW010000127         2587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_872900, whole genome shotgun sequence.
ACCESSION   DATIOW010000127 DATIOW010000000
VERSION     DATIOW010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872900"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1129)
                     /gene="serC"
                     /locus_tag="VLL03_04190"
     CDS             complement(20..1129)
                     /gene="serC"
                     /locus_tag="VLL03_04190"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HSS46599.1"
                     /translation="MNDVKGFTATKCIYNFSPGPAMLSREVLERAREEMLDWHGSGMS
                     VMEMSHRGKEFTAISTQAEADLRELMRIPAHYKVLFLQGGATLQFAMAPINLQRGKKS
                     SDYIYTGEWSKKAIAEAKKFCMVNIAASSEDAKFTYAPTQDQLRLDPNAAYVHYTPNE
                     TVGGVEFHWIPDTGEVPLVADMSSNILSRVVEVSRFGLIYAGAQKNIGPAGLTVVIIR
                     EDLIGQALPGTPSMLDYKVHVDNDSMYNTPPAYAQYIAGLNFQWLKKNGGIAAIEKRN
                     IEKARLLYDFLDNSRFFRNPVRKSDRSRMNVPFSLKNTALDEEFLKQAKAQGMIQLKG
                     HRSVGGMRASIYNAMPIEGVQTLVGFMREFEEKHG"
     gene            complement(1122..>2587)
                     /locus_tag="VLL03_04195"
     CDS             complement(1122..>2587)
                     /locus_tag="VLL03_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HSS46600.1"
                     /translation="WRAQFEHRAPHHHFAAVALSNVDEIIGLIKAAESPPVAKTQLMA
                     RHWHSKLVEDMLQRTSSDASRPEGLGPEFGLRKQGYFLSDAQAQAILELRLQRLTGLE
                     QDKIVTEYKEVMEKITDLMDMLAKRERITHIIAEELAAAKQQYGDQRRSEIVVDTQDL
                     AMEDLIAPEDVVVTLSHGGYMKSQPVADYRAQKRGGRGKQAATTKEDDFIDNLFIANT
                     HDYVLCFSNRGRIYWLKVYNVPQGSRASRGKPIVNLVPLMEHEKITAILPVREFDDQH
                     FVFMATAYGTVKKTALSAFSRPMAKGIIAVNLEDGDYLIGVALTDGKQDIMLFSDGGK
                     AVRFDEQEVRSMGRTAAGVRGMKLTRDQRVISLLTAQDEKQSVLTATENGFGKRTPIA
                     EYTRHGRGTQGMIAIQTSERNGKVVAATLVNKEDEIMLITTGGVLIRTRVSEIREMSR
                     STQGVTLINLGEGEKLAGLEKVVESDDEGSGEGTSNE"
BASE COUNT          492 a          767 c          697 g          631 t
ORIGIN      
        1 ggatttgttc atcagtgtct cagccatgct tttcctcgaa ttcgcgcata aagcccacca
       61 gcgtttgcac gccctcgata ggcatcgcgt tataaatgga tgcacgcatg ccacccaccg
      121 aacggtgtcc tttaagctgt atcattcctt gcgcttttgc ctgtttcaga aattcctcgt
      181 cgagcgcggt attcttgagg gagaacggca cattcatgcg cgaacggtca ctctttctca
      241 ccgggttgcg gaaaaaccgg ctgttatcga gaaaatcata aagcagcctg gctttttcga
      301 tgttgcgctt ttctatcgca gcgatgccgc cattcttttt cagccactgg aaattgagcc
      361 cggcgatgta ctgcgcgtaa gccggcggag tgttgtacat cgaatcgttg tccacatgca
      421 ctttgtaatc cagcattgac ggcgtgcctg gaagcgcctg ccctatcagg tcttcacgga
      481 tgatgacgac ggtcagcccc gcaggaccga tgttcttctg ggcgccggcg taaatcagcc
      541 cgaaacggct cacctccacc acgcgcgaca gaatgttcga agacatgtcg gccaccagcg
      601 gcacttcgcc ggtatcggga atccagtgaa actcaacacc gcctacggtt tcattcggcg
      661 tgtaatgcac ataggccgcg ttgggatcaa gccgcaactg gtcctgcgtc ggcgcgtagg
      721 tgaacttggc gtcttcagac gaagccgcaa tattcaccat gcaaaatttt tttgcctcgg
      781 ctatcgcctt tttcgaccat tcaccggtat aaatatagtc agaggatttt ttgccgcgct
      841 gcagattgat gggcgccatg gcaaactgca gggtggcgcc gccttgcagg aacagcactt
      901 tgtaatgggc cggaatgcgc atcagctcgc gcaaatccgc ttcggcctgc gtagagatgg
      961 ccgtgaattc cttgccgcgg tggctcattt ccatcaccga cattccgctg ccatgccaat
     1021 ccagcatttc ctcgcgtgcg cgctccagaa cctcccggga caacatggca gggccgggac
     1081 tgaagttgta gatgcatttt gttgcggtaa atccttttac atcattcatt agaggtccct
     1141 tctccactgc cttcatcatc gctctccacg actttttcca ggcctgcaag cttttcacct
     1201 tctccgaggt tgatcagcgt cacaccctgg gttgagcggc tcatttcgcg tatctccgaa
     1261 acgcgggtgc ggatcagcac gccgccggtg gtaatcagca tgatttcatc ctctttattc
     1321 accaacgtcg ccgccaccac cttgccattg cgctctgagg tttgaatcgc gatcatgcct
     1381 tgcgtgccgc ggccgtggcg cgtgtattcg gcaattggcg tgcgtttgcc aaagccgttt
     1441 tcggttgcgg tcagcaccga ctgtttttca tcctgtgcgg tgagcaatga aatcacgcgc
     1501 tgatccctgg tgagcttcat gccgcgcaca cccgccgcgg tgcggcccat cgagcgtact
     1561 tcctgctcgt caaaacgcac agccttgccg ccgtccgaga atagcatgat gtcctgcttg
     1621 ccgtcagtga gcgccactcc gatcagatag tccccgtctt ccaggttcac cgcgataatg
     1681 cctttcgcca ttggacggga aaatgccgag agcgcggtct ttttgaccgt gccgtaggcg
     1741 gttgccatga atacgaagtg ctggtcgtcg aattccctca ccggcagaat cgcggtgatt
     1801 ttctcgtgtt ccatcagcgg cacgagattg acgatgggct tgccgcggct ggcgcggcta
     1861 ccctgcggca cattgtaaac cttgagccag taaatcctgc cgcgattcga aaagcacaga
     1921 acgtaatcgt gggtgttggc gatgaaaagg ttgtctatga aatcatcttc cttggtggtt
     1981 gccgcctgct tgccgcgccc gccgcgtttc tgcgcccggt aatcggcaac cggctgtgat
     2041 ttcatgtacc cgccgtgcga taatgtgacc acgacgtcct ccggtgcaat caggtcttcc
     2101 attgccaggt cctgggtatc caccacgatt tcgctgcggc gctggtcgcc atactgctgt
     2161 ttagccgcgg caagctcctc ggcgatgatg tgggtgatgc gttcgcgttt cgccagcata
     2221 tccatgaggt cggtgatttt ctccatcact tccttgtatt cggtgacgat tttgtcctgc
     2281 tccaggccgg taaggcgctg caggcgcaat tccaggatcg cctgagcctg cgcatcggac
     2341 aggaaatagc cctgcttgcg caggccgaat tccgggccga gcccctccgg acgcgaagca
     2401 tcggaactcg tacgctgcaa catgtcctcg accagcttgg aatgccaatg gcgggccatc
     2461 aattgcgttt tcgccacggg aggcgattcc gcggccttga tgagcccgat gatttcatcc
     2521 acattggaaa gcgccaccgc cgcgaagtgg tgatgcggcg cacggtgttc gaattgcgcc
     2581 cgccaat
//
LOCUS       DATIOW010000128         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_364073, whole genome shotgun sequence.
ACCESSION   DATIOW010000128 DATIOW010000000
VERSION     DATIOW010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364073"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VLL03_04200"
     CDS             complement(<1..259)
                     /locus_tag="VLL03_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HSS46601.1"
                     /translation="MATMRVVQVSRPNGPFEIVERQIPEPAAGSVRIQVQACGICHSD
                     SLTKEGTFPGIQYPRVPGHEVAGVIDAVGTGVAGWEPGQRVG"
     gene            complement(331..579)
                     /locus_tag="VLL03_04205"
     CDS             complement(331..579)
                     /locus_tag="VLL03_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46602.1"
                     /translation="MVDILTLLGLVLNFVGALLLMFFRFPALDVTADGRSLGQWQNEP
                     TPEERSKNLRRYWRNEVATRAGLICLCVGFVFQLLAFI"
     gene            905..1477
                     /locus_tag="VLL03_04210"
     CDS             905..1477
                     /locus_tag="VLL03_04210"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011901993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HSS46603.1"
                     /translation="MLLMIDNYDSFTYNLVQYLGELGEEVVVYRNDEIALDKIAQLKP
                     AHIVISPGPCTPNEAGVSVPLIKRFKGEIPILGVCLGHQSIGQAFGGRIVHAKKLMHG
                     KTSQIKHSERGVFRNLPNPFQATRYHSLVIERERLPDCLEVTAWADDGEIMGVRHKQL
                     KIEGVQFHPESILTEHGHDLLRNFLREENK"
     gene            1474..>2281
                     /gene="trpD"
                     /locus_tag="VLL03_04215"
     CDS             1474..>2281
                     /gene="trpD"
                     /locus_tag="VLL03_04215"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HSS46604.1"
                     /translation="MTPQEALNRLIERREIFHDEMLSIMRQIMSGQLTPAQIAGIIMG
                     LRVKGETVEEIAAAAQVLRELSTRVDVSGEPHLVDTCGTGGDGARTFNISTTSAFAAA
                     AAGAKVAKHGGRSVSSSCGSADVLEALGVNINLNARQVAQSIHKIGIGFMFAPNHHSA
                     MKHAASVRKELGVRTLFNILGPLTNPAGAKNQLLGVFHHDLVGIQARVLQALGSRHAL
                     VVYGVEGLDEISISGETEVAELKNGRVEEYTLDQDRFGIKLAPLEAIQAKN"
BASE COUNT          530 a          658 c          636 g          457 t
ORIGIN      
        1 cgcccaccct ctggcccggc tcccacccgg cgacgccggt gccgaccgcg tcgatcactc
       61 cggccacctc atggccgggg accctcgggt actggatgcc cggaaaagtt ccctccttgg
      121 tgagcgaatc gctgtggcag atgccgcagg cttgcacctg gatccgtacc gatccggcgg
      181 ccggctcagg tatctgccgt tcaacgatct cgaagggccc gttagggcgc gagacctgca
      241 ccacgcgcat cgtcgccatg atttctccaa cctcagctga acaaaggcat aaccacgaaa
      301 acaaatagtg ttcccggagc ccgcgtcgga ttaaatgaac gctaataact ggaacacgaa
      361 accgacgcaa aggcagatta gcccggcccg tgttgcgact tcgtttctcc aataccgcct
      421 caagtttttc gagcgctcct cgggcgtagg ctcattctgc cattgaccca agctccgacc
      481 gtcggcagtt acatcgagtg ccggaaagcg aaaaaacatg agcagcagcg cacccacaaa
      541 gttcaagact aagccgagca gggttagaat gtccaccatt tttgctcctt tccgatcttc
      601 ctgggatatt gaaaaccgat caattttact ccaccgtggc gtaattacgg cagtgggact
      661 ctagattagc gctgctgctt tgggttacac tttctttcgt ggcaactcat ccattcctgc
      721 cgatctgcat caacccgctc ccgcgatggc gatagtctgc ctcgccccgt aaaaacgatg
      781 tggcttcccc ttcagttgta agatagttcc gccccagtat cgctgaggaa agcggatgtt
      841 tatgagggta tcgaggcagt atttgccaaa ttagatatat ataaatcaat ttgataagct
      901 aattatgctt ctgatgatcg ataactacga ttcgttcacc tacaacctgg tgcaatacct
      961 cggcgaacta ggtgaagagg tcgtcgtgta ccgcaacgac gaaatcgcgc tcgataaaat
     1021 cgcgcagctc aagcccgcgc atatcgtgat ttcgccgggg ccgtgcacac ccaacgaagc
     1081 cggggtgtcg gtgccgctga tcaagcgttt caagggagaa attccgatat tgggcgtgtg
     1141 tctcggccat cagagcatag gccaggcgtt cggcggcagg atcgtgcatg cgaaaaagct
     1201 catgcacggc aagacttcgc aaatcaagca cagtgagcgc ggcgtattcc gcaatcttcc
     1261 caatccgttt caagccacgc gctatcattc gctggtgatt gaacgcgaac gcctgccgga
     1321 ttgcctggag gtcactgcct gggccgacga cggcgaaatc atgggtgtgc gccacaagca
     1381 attgaaaatc gagggcgtcc agttccatcc ggaatcgatt ctgaccgaac acggtcacga
     1441 cctgctcagg aattttttgc gggaagaaaa taaatgacgc cgcaggaagc cctgaaccgc
     1501 ctgattgagc ggcgtgaaat ttttcatgac gaaatgctgt ccatcatgcg ccagatcatg
     1561 agcgggcaac ttaccccggc gcaaatcgcc ggtatcatca tgggcctgcg cgtgaaaggc
     1621 gagacggtcg aagaaatcgc cgctgcggca caagtgttgc gcgagctttc aaccagagta
     1681 gatgtctccg gcgagccgca tctggtcgat acttgcggca ccggcggcga cggtgcgcgc
     1741 acgttcaaca tctccaccac ttcggcattc gccgcggcgg cggcgggggc gaaagtggcg
     1801 aagcacggcg ggcgctcggt atccagcagc tgcggcagcg ccgacgtgct ggaagcgctg
     1861 ggggtcaata tcaatctcaa tgccaggcag gtggcgcagt cgatacacaa gatcggcatc
     1921 ggcttcatgt ttgcgcccaa ccatcacagc gcaatgaagc acgccgcgtc ggtgcgcaag
     1981 gaactcggcg tgcgcacatt gttcaacatt ctgggaccgc tcaccaatcc ggcgggagct
     2041 aaaaaccagt tgctgggcgt gtttcaccac gatttagttg gcatacaagc gcgcgtgctg
     2101 caagccttgg gcagccggca cgcgctggtg gtctacggcg tcgaaggcct ggatgaaatt
     2161 tccatcagcg gcgagaccga agtggccgag ctcaaaaacg gcagagtgga agaatacact
     2221 ctcgaccaag accgctttgg aatcaagctt gcgccgctgg aagcaattca ggcaaaaaac
     2281 g
//
LOCUS       DATIOW010000129         4028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1481918, whole genome shotgun sequence.
ACCESSION   DATIOW010000129 DATIOW010000000
VERSION     DATIOW010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481918"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VLL03_04220"
     CDS             <1..946
                     /locus_tag="VLL03_04220"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HSS46605.1"
                     /translation="RRALQLPRGYNPRARALAESWRQTLTDDAAVIKQALKMFSSEKF
                     FYTLQPPLLGKNPVDEFLFDTKRSFCEHYASSFAFLMRVAGIPTRVVTGYQGGELNEL
                     GNYLIVGQADAHAWTEVWLKDRGWVRVDPTAAVSPLRVEAGIAAAVPKTDSLPLLVRQ
                     DLQWLRQARLIWDAMANNWNQWVLGYNPQRQKQFLSDLGMSEATWQNMAIALLVAAGL
                     VMLGLAGMILWRLKATATDPVQKAYSKFCGKLAKQGLPRHPAEGPQDYALRLAQAKPL
                     QAEPIRSISELYIALRYGTLAGKPAAQRLRQLVSDFEP"
     gene            977..1435
                     /locus_tag="VLL03_04225"
     CDS             977..1435
                     /locus_tag="VLL03_04225"
                     /inference="COORDINATES: protein motif:HMM:NF024818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSS46606.1"
                     /translation="MTINQAGAIESFNSGASKIFGYSADEIIGKNIKILMPEDGSVSS
                     ACGMECYLKTGEAHVPGKSGTEFRGRRDDARRIAEKIISTLRPAFSLEHCQVNTTVSI
                     GITFFSGSKDISGDTLVKMADSAMYQAKQKGRNNYEVYQYRASGSTEERP"
     gene            complement(1416..2300)
                     /gene="rpoS"
                     /locus_tag="VLL03_04230"
     CDS             complement(1416..2300)
                     /gene="rpoS"
                     /locus_tag="VLL03_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HSS46607.1"
                     /translation="MTAPAEEPVAEVDHFTADVLSDVTQIYLNEIGQNALLSAEDELR
                     YARLAKQGDFKARQKMIEHNLRLVVNIAKHYVHRGMALLDLIEEGNLGLIHALEKFDP
                     ERGFRFSTYATWWIRQNIERAIMNQSRTIRLPVHIIKELNIVLRALRHLETHCEREPT
                     NEDVAHLLGWSVEDVRKMLLLNERVASLDAPLDIDPMLSIGESIPDENNPMPDVRLEH
                     SELENYVREWLDQLNEKQRRVIEHRYGLNGYEISTLEQLAESLGLTRERVRQIQIEAL
                     YSLRKILKRKGVSKDVLL"
     gene            complement(2342..3310)
                     /locus_tag="VLL03_04235"
     CDS             complement(2342..3310)
                     /locus_tag="VLL03_04235"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HSS46608.1"
                     /translation="MMKYFLFVKNSLLFLAALMITGCVSAPGKAPVIEKSPQAKEAAK
                     PLVKAKPVAQEGEWRPEFYTMQKGDTLYSIALEHGLDYKDLAQWNNIDNPAAISAGQQ
                     LRLTPPPDAAVTAPLQVTPGVEGQPVAGAIVEAKPLPSPGFVKSEPKAVKLPYSDQAL
                     AQLQKPETARITSEPPKEVKPEVKAAAPASSIVDGIDWGWPANGKIVAGYSESANLKG
                     VDIAGKLGQPVYAGAAGRVIYSGGNLRGYGKLIIIKHNDVYFSAYAHNSEILVKEGQT
                     VVKGQKIAEMGNTDADQVKLHFEIRRLGKPVDPAKYLPDPASDKTS"
     gene            complement(3307..>4028)
                     /locus_tag="VLL03_04240"
     CDS             complement(3307..>4028)
                     /locus_tag="VLL03_04240"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HSS46609.1"
                     /translation="RLISPSLRRWKASGAGLRTSSMTARHSGIGMTSERTRTRMVERL
                     RSQGILDEIVLTAMATVPRHIFVDEALASRAYEDVSLPLGFGQTISSPYTVARMLELL
                     RGGKPLKRALEVGTGCGYQAAVLACLADEVYSLERIASLSTKARKRLHELRISNVRVR
                     QVDGHGGFPDAAPFDAIIVAAAATHVPAPLLEQLTVGGRMVLPMGIQEQKLCVIERNS
                     QGCTQTVLEAVKFVPLRSGME"
BASE COUNT          807 a         1206 c         1158 g          857 t
ORIGIN      
        1 aaggcgcgcg ctgcagcttc cgcgcggcta caatccgcgc gctcgcgcgc tggccgaatc
       61 ctggcggcaa actctgaccg atgatgccgc cgtgattaaa caagcactga aaatgtttag
      121 cagcgagaag tttttctata ctctgcagcc gcctctgctg ggcaaaaatc cggtggatga
      181 atttttgttc gacaccaagc gcagcttttg cgagcactat gcttcaagct tcgcgtttct
      241 gatgcgcgtg gcagggattc ctacgcgcgt tgtcaccggc taccagggcg gtgagcttaa
      301 cgagctcggc aactatctca tcgtcggcca ggcggacgcg cacgcctgga ccgaagtgtg
      361 gctcaaagac cggggctggg tgcgggtcga tccgactgcg gcggtctcgc ccttgcgggt
      421 cgaagccggc atcgccgccg ccgtgccgaa aaccgattcc ctgccgttgc tggtgcggca
      481 ggatttgcaa tggttgcgcc aggcgcgctt gatctgggat gcgatggcga acaactggaa
      541 tcaatgggtg ctgggctaca acccgcagcg ccaaaaacag tttttgtcgg atctgggcat
      601 gagcgaagct acttggcaaa acatggcgat tgctctactt gtcgccgccg gcctggtcat
      661 gctagggctg gccggaatga tcttgtggcg cctcaaagcc accgctaccg acccggtgca
      721 gaaagcctac tccaaattct gcggcaagct ggccaagcag ggcctgcccc gccacccggc
      781 ggaaggacca caggattacg cgctgcggct tgcgcaagcc aaaccgctgc aggctgagcc
      841 gatacgcagc atcagcgaac tctatattgc attgcgttac ggcaccctgg ctggcaaacc
      901 ggcagcgcag cgtttgcggc agctggtgag cgattttgaa ccttgaacag gtgatgcgct
      961 ggataaggat ggggtcatca ctatcaacca ggcaggcgcc atcgaatcgt ttaattccgg
     1021 cgcatcaaag atatttggct actctgccga cgaaatcatt gggaaaaata tcaaaatact
     1081 aatgccggaa gacgggtctg tttcttctgc atgcggtatg gaatgttacc tcaaaaccgg
     1141 cgaagcgcat gtgccgggca aaagcgggac ggagttccgg gggcggcgcg atgatgcgag
     1201 gcgtatcgcc gaaaaaatca tcagcacctt gcgccccgca ttctcacttg agcattgcca
     1261 ggtcaacacc actgtcagca tcggcatcac gttcttttcc ggcagcaagg acataagcgg
     1321 cgatacgcta gtcaagatgg cagacagcgc catgtaccaa gccaagcaga aggggcgcaa
     1381 caactacgag gtataccagt accgcgcctc aggctctaca gaagaacgtc cttagacacg
     1441 cccttgcgct tgagtatctt gcgcaggctg tagagcgcct cgatctggat ctgccgtacc
     1501 cgctcgcgcg tgagacccag actctccgcc agttgctcaa gggtggagat ttcgtagccg
     1561 ttcaacccgt agcgatgctc gatcacccgc cgctgttttt cattcaactg gtcgagccac
     1621 tcccgcacgt agttttccag ttccgagtgc tcgagcctga catcgggcat ggggttgttt
     1681 tcatcgggga tggactcgcc gatggaaagc atgggatcaa tatccagcgg cgcatccagc
     1741 gaggccacgc gctcgttaag caacagcatc ttgcgcacgt cttccaccga ccagccgagt
     1801 agatgcgcca catcctcatt agtcggctcg cgctcgcaat gcgtctcgag atggcgcagc
     1861 gcgcgcagca cgatgttgag ctccttgatg atgtgcaccg gcagccggat agtgcgcgac
     1921 tggttcatga tggcgcgttc gatgttctgg cggatccacc acgtggcata ggtggaaaag
     1981 cggaaacctc gttccgggtc gaatttctcc agcgcgtgaa tcagacccag attgccctct
     2041 tcaatgagat cgagcagcgc catgccgcga tgcacatagt gcttggcgat gttcaccacc
     2101 aagcgcaggt tgtgctcgat catcttctgc cgcgccttga aatcgccctg cttggcaagc
     2161 cgcgcgtagc gcaattcgtc ttccgccgag agcagcgcgt tctggccgat ttcattgaga
     2221 taaatctgcg tgacgtcgga cagcacgtcg gcggtaaagt ggtccacttc cgccacgggt
     2281 tcctcggctg gcgccgtcat gccttcgtcg agcggctcgt cttcagagga aaaattattg
     2341 ttcatgatgt tttatcgcta gccggatcgg gcaggtattt ggccggatca accggtttgc
     2401 ccagacgccg gatttcaaaa tgcagcttca cctggtcggc gtcggtattg cccatctcgg
     2461 caattttctg gcccttgacc accgtttgcc cctctttcac cagaatctcg ctgttgtgcg
     2521 cgtaggcgct gaaatagaca tcgttgtgct tgatgataat cagtttcccg tagccgcgta
     2581 aattgccgcc gctatagatc accctgccag ccgcgccggc atacaccggc tgccctagct
     2641 tgccggcgat gtccacgccc ttgagattgg cgctctcact gtaaccggca acaattttgc
     2701 cgttcgccgg ccagccccag tcaatgccgt ccacgatgga gcttgccggc gctgccgcct
     2761 tgacttccgg cttgacctct ttcggcggct cgctcgtgat acgcgcagtt tcgggttttt
     2821 gcaattgggc aagggcttgg tcggaatacg gaagcttcac cgccttgggt tcggatttca
     2881 caaaacccgg ggacgggagc ggcttggctt cgacgatggc acccgctact ggctggccct
     2941 ccacccccgg cgtcacttgc aagggagccg tcacggctgc atccggcggt ggtgtaagcc
     3001 gcaattgttg gccggcgctg atcgccgccg gattatcaat attgttccat tgcgccaggt
     3061 ccttgtaatc cagcccgtgc tccagcgcga tgctgtagag cgtgtcgcct ttctgcatgg
     3121 tataaaactc gggacgccat tcgccttcct gggcgacagg tttagctttg actaaaggtt
     3181 tggcggcttc cttggcttga ggtgattttt caattaccgg cgccttgccc ggtgcactga
     3241 cacagcctgt aatcatcagc gccgccaaaa acagcaaact atttttcacg aacaagaaat
     3301 atttcatcat tccatcccgg accgcagcgg cacaaatttc accgcttcca aaaccgtctg
     3361 cgtgcaacct tgcgagttgc gctcgataac acacaacttt tgttcttgga ttcccatcgg
     3421 cagaaccatt ctaccaccaa ccgtcagttg ctcgagcagc ggcgcgggca catgggtggc
     3481 ggcggcggcg acgataatcg cgtcaaaagg cgcggcatcg ggaaaaccgc cgtggccgtc
     3541 cacctgccgc acgcgcacgt tgctgatgcg caactcatgc aggcgcttgc gggccttggt
     3601 ggacagcgaa gcaatgcgct cgagggaata cacttcatcg gcgaggcacg ccagcacggc
     3661 cgcttggtaa ccgcaaccgg tgccgacttc cagcgcgcgt tttaacggtt tgccgccgcg
     3721 cagcagttcc agcatgcgcg ccacggtgta cgggctggaa atggtctgac caaagcccag
     3781 cggcagcgag acatcctcat aagcgcggct cgccagcgcc tcatccacga aaatgtggcg
     3841 cggcactgtc gccatggcgg tgagcactat ctcgtcaagt atgccctggc tgcgcaggcg
     3901 ctccaccatg cgcgtacgcg tgcgctctga agtcatgccg atgccggagt gccgcgcggt
     3961 catgctagaa gtcctcaagc cagcgcctga ggctttccat ctgcgcaaac tgggtgagat
     4021 caatctgt
//
LOCUS       DATIOW010000130        10129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1079310, whole genome shotgun sequence.
ACCESSION   DATIOW010000130 DATIOW010000000
VERSION     DATIOW010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10129
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079310"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2137)
                     /locus_tag="VLL03_04245"
     CDS             complement(<1..2137)
                     /locus_tag="VLL03_04245"
                     /inference="COORDINATES: protein motif:HMM:NF026042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46610.1"
                     /translation="MDSTKPRMTTHQETALKLRHGLSFEDLYRRDGLLRLDAAFLNFL
                     GETDAALRQRIEVGRANPAALAKKEESELLIAVAPHLDDFIADLFGIESEVQKLSARH
                     HELAPLYSCKRLFVQRKAMHKVKPEEAAAVDGRAVEKRLAALFGEPFSELAFAKHVSE
                     WQKDDVANADKLQTALLYAAWAATTPAGRQRHHSGVLFKAPHKLDFMRLVPVVTDESQ
                     GFNAFTLDKKHLRRREGFGLTDPGTDLVGALDEANYCIWCHEQGNDSCSTGLREKPQA
                     DGCAPFKKTVFGVTLAGCPLEEKISEFHKVKTEGQAIGALAMIAVDNPMVAATGHRIC
                     NDCMKSCIYQKQEPVNIPQVETRTLKDVLELPWGFEIYSLLTRWNPLDLRRPYPKAPT
                     NRRVLIACMGPAGFTLAHYLMNDGHTVVGIDGLKIEPLPAEISGVDAHGKRVPFKPIR
                     DIGELYESLDDRVMAGFGGVAEYGITVRWDKNFLKIIRLLLERRSQFAMFGGVRFGGT
                     LTVDEAFMQGFDHIALAAGAGRPTVIDMPNALARGVRTASDFLMALQLTGAAKADSIA
                     NMQLRLPVVVIGGGLTAIDTATESLAYYPLQVEKFLLRYETLVAERGVAAVRRNWHPE
                     ERYIADEFIEHAKAIRAERAAAQAKGRKPRLLELLQSWGGVTIAYRKRLIDSPSYTLN
                     HEEVEKALQEGVKFAEGLNPLRVEVDSFGA"
     gene            complement(2297..3319)
                     /locus_tag="VLL03_04250"
     CDS             complement(2297..3319)
                     /locus_tag="VLL03_04250"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HSS46611.1"
                     /translation="MLYSLLRPLLFRLDPETSHHLTLELLRCTSGLNRLFAKAAAGKP
                     CQVMGLDFPNPVGLAAGLDKNGEYIDALATLGFGFIEVGTVTPRPQPGNTRPRLFRVP
                     QARALVNRMGFNNHGVDRLIENIRRARYKGILGINIGKNADTPMENAADDYIACLRKV
                     YPFASYVAINISSPNTRGLRTLQQKQELDSLLSVLKEEHKKLAKLFGKQAPLVLKIAP
                     DLSEQEISDIAELLLAHKIDGVIATNTTVARAGIEGLSQESGGVSGAPLNEKSTAVIR
                     QLAAALQGQLPIIGVGGIMSGANASEKLQAGASLVQIYSGLIYRGPALVAEIIESLTE
                     QTGVAA"
     gene            complement(3333..4073)
                     /locus_tag="VLL03_04255"
     CDS             complement(3333..4073)
                     /locus_tag="VLL03_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HSS46612.1"
                     /translation="MKSAQTQGQDSASRFYFLHLVIPLIAAGLVLWVIYLLGVDERIT
                     RLFFDPVTQQFPLRNRWFLEVVMHRWAKYAVVILGVAVLAAYLVSFRIASLAPLRRVF
                     LFLILAMGLSSGAVSYLKSVSHKQCPYDLKMYGGYAPYTGIFEKAPPGVKPGRCWPGG
                     HSSAGFCLMAFYFVGLRLRNKRVALWGLYGGFGLGFVFGMGRVLQGAHFLSHHLWAAL
                     ICWLVMLALYELMLRRLPIATREITVQT"
     gene            complement(4099..6045)
                     /locus_tag="VLL03_04260"
     CDS             complement(4099..6045)
                     /locus_tag="VLL03_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HSS46613.1"
                     /translation="MRSIISSSGGLLRRIYESSRFGILYLAATLYLIISFLTRIVLAV
                     RPIKEGQIAVGSLAAILPIGTLYDLITCLYLFAPFALYLFLFPQKAFRSIGQRVFLIT
                     AFAASLYGLIYLGAVEYYFFDEFNSRFNFVAVEYLVFPHEVFVNIWESYPVGWALIAT
                     ALATFAVMWRFYPMLNRSMLKEERSAARVKPFGTWMILLLIVHATVNINTGRYSENRV
                     ANELTANGIYSFFNAAMNSEMDYPNFYTTVSAEEAGRRLRQLVAQSNSTFISGSNNPI
                     ARHIENPGKPLLLNVVILLEESLGADFVGAYGDTRGLTPNIDRVAKDSVVFTNVYATG
                     TRTVRGMEAVSASFPPVPAEAIVKRPNNEGMFNWSTVMKKNGYAPTFIYGGYGTFDNM
                     NYFFGNNGYRVVDRTDMDTPKFSNIWGVSDEDLFRNALRIFDTQHARGEKIFSIVMTT
                     SNHKPFTFPEGVPGVPAKGGGREAGVRYADYAIGKFFDAIKNRPYFNDTLVIIVGDHG
                     ARVYGREDIPLRSYELPLLIYAAGHFKPYKMDTLTSQMDIAPTVLGMLNISYDSVFFG
                     KDVFKSDPKDRFVLLNHNRDIALYDGTKLTELNFRKISGTYLYDKKTNRQNKTALDQE
                     QMKNAASVFQSAYDFYIHRRYGLH"
     gene            complement(6042..6779)
                     /locus_tag="VLL03_04265"
     CDS             complement(6042..6779)
                     /locus_tag="VLL03_04265"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HSS46614.1"
                     /translation="MTYLNEFPLSVLQFYATAPYPCSYLTGKMARSQVATPSHLIDTS
                     VYSELVKTGFRRSGAFTYRPHCDSCRACVPVRLIAGAYCPTRTQRRTWKRHQNLRAEM
                     LELKYHPEHYALYLRYQAKRHSGGGMDQDSREQYQHFLLQSNVDSQLVEFREGRDLRM
                     VSIIDRLHDGLSSVYTFFEPNLPGASLGTYNIMWQIELCRNLGLQYLYLGYWIGESRK
                     MAYKANFQPIEGLINDRWQSLPAPTRR"
     gene            complement(6763..7461)
                     /gene="aat"
                     /locus_tag="VLL03_04270"
     CDS             complement(6763..7461)
                     /gene="aat"
                     /locus_tag="VLL03_04270"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HSS46615.1"
                     /translation="MICWLGRNTPFPPSEQALHEPNGLLAGGGDLSLKRLVEAYSKGI
                     FPWFNEGEPILWWSPDPRMVLFPAELKISRSLRKVLKKRDYEIRADTAFAEVMKSCAA
                     PRRGQPGTWITPDMMQAYQELHRVGLAHSLETRIGGKLAGGLYGVALGRVFFGESMFA
                     RRTDASKIAFVHLVRQLSRWGFGLIDCQMKTAHLASFGAREIPRKQFTRTLEELINYA
                     GTQGKWRLDHDLFE"
     gene            complement(7551..8093)
                     /locus_tag="VLL03_04275"
     CDS             complement(7551..8093)
                     /locus_tag="VLL03_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HSS46616.1"
                     /translation="MRILIAALSCSLMVAGCAWMPGWLSGKTATANLQPTKGNKVGGT
                     VTFTQKGDKVLVVAKVNGLKPGMHGFHVHEKGDCSAADGMSAGGHFNPQGKQHGGPDS
                     ADHHAGDLGNLNADSSGNAAYSYEAPGITLDKSAANSIVGRSVIVHANRDDYTPPAGN
                     SGGRIACGVIVLNEAPRSGY"
     gene            complement(8139..8846)
                     /locus_tag="VLL03_04280"
     CDS             complement(8139..8846)
                     /locus_tag="VLL03_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HSS46617.1"
                     /translation="MERREFIKICAAGAAVAAVNSGFAAQNTEPRFYARARLVDYDGR
                     PLQASRLQIKRNYIFHYPFQGTPCFLLNLGKPTATDALLATADGRNYHWRGGVGAGSS
                     IVAYSAICAHRLAYPTKQISFISYRADKTEASVGHINVIHCCAEHSEYDPAQGAKVVS
                     GPAPQPLAAIILEYDAKTDELFAVGTLGGELFNDFFSKYDFRLSLEYGSNKAQKPVTS
                     DVIITELTNFCQQQVRC"
     gene            9061..9459
                     /locus_tag="VLL03_04285"
     CDS             9061..9459
                     /locus_tag="VLL03_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46618.1"
                     /translation="MPELSPEVAGLLKELRTFLANGSEPWEQRRELMLKINNLVEGGE
                     TDLFVGMAREMSRIGATYTNLLQAFAQFGAAQQGGANLADWFMRSFQQPQAMVPPFMS
                     PAAGFDYFKQLADFMSAQAKSAGASQKKKD"
     gene            9486..9755
                     /locus_tag="VLL03_04290"
     CDS             9486..9755
                     /locus_tag="VLL03_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46619.1"
                     /translation="MQMSRDGMPAETIVKQLRDSREVYRLSASQLVKLHDQGVADEVI
                     DYMYMQATYIQAERGEEIQYQSQFCGYGLYCRPFPYLPPALMRMC"
BASE COUNT         2176 a         2781 c         3008 g         2164 t
ORIGIN      
        1 ccgcgccaaa tgaatccact tccacgcgca acgggttcaa gccttcggcg aatttaacgc
       61 cctcctgcag tgctttttcc acttcctcgt ggttcaaggt atacgagggg ctgtctatca
      121 ggcgcttgcg gtaagcgatg gtcacgccgc cccagctttg cagtaattcc aggaggcgcg
      181 gtttacggcc tttggcttga gcggctgcgc gctcggcgcg gatggcttta gcgtgctcga
      241 taaattcatc ggcgatatac cgttcttcgg ggtgccagtt gcggcgtact gcggccacgc
      301 cacgctccgc cactagcgtc tcatagcgca gcaggaattt ctcgacctgc aacggataat
      361 aagcaagtga ttcggtggca gtgtctattg cggtcaagcc gccgccgatt accaccaccg
      421 gcagtctgag ctgcatgttg gcaatcgagt ccgcttttgc cgcgccggtc aattgcagcg
      481 ccatcaggaa atcggaagcg gtgcgcaccc cgcgtgcaag tgcgttgggc atgtctatca
      541 ctgtcgggcg tccggcgcct gccgccagtg caatatggtc gaagccttgc ataaaagctt
      601 cgtctaccgt gagcgtgccg ccaaagcgca cgccgccaaa catcgcgaac tggctgcggc
      661 gctccaaaag caggcgaatg atcttgagga aattcttatc ccagcgcacg gtgatgccgt
      721 attcggccac gccgccgaaa cccgccatca cccggtcatc cagcgattcg tacaattcgc
      781 caatatcgcg gatcggctta aacggcacgc gtttgccgtg tgcatccacg cctgaaattt
      841 cggcgggcag cggttcgatc ttcaagccgt cgatgccgac caccgtatgg ccgtcattca
      901 tcagatagtg cgccagagtg aaaccggcag gccccatgca tgcaataagc acgcggcgat
      961 tagtcggcgc tttcggatag ggacgccgca gatcgagcgg attccagcgg gtgagcaggc
     1021 tgtaaatttc aaagccccac ggcagctcca gcacatcttt cagcgtgcgc gtctccacct
     1081 gcgggatgtt gaccggttcc tgcttttggt aaatgcagga cttcatgcag tcgttgcaga
     1141 tgcggtgtcc cgtggcggcg accatcgggt tgtccaccgc aatcatcgcc agcgcaccga
     1201 tcgcctggcc ttcagtcttc actttgtgga attcggaaat tttttcttca agcggacagc
     1261 cggcaagcgt tacgccgaac acggttttct tgaacggtgc gcagccgtcg gcctgcggtt
     1321 tttcgcgcag gccggtggag caggaatcgt tgccctgctc gtggcaccag atgcagtagt
     1381 tggcctcgtc cagcgcgccg accaaatcag tccccgggtc ggtgagcccg aacccttctc
     1441 ggcggcgcag atgcttttta tcaagcgtaa aggcgttgaa gccttggctc tcgtcggtga
     1501 caaccggcac caggcgcatg aaatcgagtt tgtgaggcgc cttgaacaat acaccgctgt
     1561 ggtggcgttg gcgccccgcc ggcgtggtcg ccgcccaagc ggcgtagagc agagcggttt
     1621 gcagcttgtc ggcgtttgcc acgtcatctt tttgccattc gctgacatgc ttggcgaacg
     1681 cgagctcgga aaacggctcg ccaaacagcg ccgccaaccg cttctcgacc gcgcggccgt
     1741 caacggcagc agcttcctcg ggtttcacct tgtgcatcgc cttgcgctgg acgaacaggc
     1801 gcttgcagga ataaagcggc gcaagctcgt gatgccgggc ggaaagtttc tgtacctcgc
     1861 tttcgatacc gaataaatcg gcgatgaaat catcgagatg cggtgccact gcaatcagca
     1921 gttccgattc ttcttttttc gccagcgctg cagggttggc gcgcccgact tcaatgcgtt
     1981 ggcgcagcgc ggcatctgtt tcgccgagaa aattcagaaa cgcggcgtca agccgcagca
     2041 aaccgtcccg gcggtagagg tcttcgaaag aaagcccgtg acgcagctta agcgctgttt
     2101 cctgatgcgt agtcatgcgt ggcttggtgg aatccattta aaagcatgca ttatcgcttt
     2161 taaggatgcg ccattcaacc atgctgaggt tatatagata tatgtttgga ttatatgctg
     2221 gggcaaggtg ctgggtgtcg cggcggcggc gatgcgcggc taggttatgc gggatggact
     2281 gcggagcaca tctatgtcag gcagcaacgc cagtttgttc ggttagtgat tcaatgattt
     2341 cagccaccag cgccgggccg cggtaaatca agccgctgta aatctgcacc aggcttgcgc
     2401 cggcttgcag cttttccgat gcgttcgcgc cgctcatgat gccacccacc cctatgatag
     2461 gcagttgccc ttgcagcgca gctgccagct gacggatgac ggcggtggat ttttcattaa
     2521 gcggtgcgcc gctaacaccg ccgctttcct ggctcaagcc ctcgattccg gcgcgcgcaa
     2581 ccgtggtgtt ggtggcgatc acgccgtcga ttttatgcgc cagcaacagc tcagcgatgt
     2641 cgctgatttc ctgttcgctg agatcgggag cgattttcag caccagcggc gcctgttttc
     2701 caaacagctt ggcaagtttc ttgtgttctt cctttaagac ggacagcaac gaatcgagtt
     2761 cctgtttttg ctgcagtgtg cgcaagcctc gcgtgttcgg tgaggaaatg ttgatggcga
     2821 cataactcgc aaacggatac accttgcgca agcaggcgat atagtcatcg gccgcgtttt
     2881 ccatcggcgt gtcggcattc ttgccgatgt tgatgcccaa tatccctttg tagcgcgcgc
     2941 ggcggatgtt ttctatcaat ctgtccacgc cgtgattgtt aaaacccatg cggttgacga
     3001 gggcccgggc ctgcggaacc cgaaacaatc gcgggcgcgt atttcccggc tgagggcggg
     3061 gtgtcacggt accgacttcg atgaaaccga aacccagcgt ggcgagcgcg tctatgtatt
     3121 cgccattttt gtccagtccc gctgcaagtc ccacgggatt gggaaaatcc agtcccatga
     3181 cttggcaagg cttgccggca gccgcttttg cgaacaggcg gttcaagccg gaggtgcaac
     3241 gcaaaagctc aagggttaaa tgatgggaag tttcaggatc aagccggaac aacaaagggc
     3301 gaagcagtga gtaaagcatg gcgggaattt tatcaggttt ggaccgtgat ctccctcgtg
     3361 gcgatgggta aacggcgtaa catcagctca tagagtgcca gcatcaccag ccaacaaatc
     3421 agcgccgccc ataaatggtg cgagagaaaa tgcgcgcctt gcagcacccg tcccatgccg
     3481 aaaacgaaac ccaatccgaa accgccgtac agtccccata aggctacgcg tttattgcgc
     3541 aggcgcaagc cgacgaaata aaacgccatc aggcaaaagc cggcggaaga atgcccgccg
     3601 ggccagcacc tgccgggctt cacgcctggc ggcgcttttt cgaaaatacc cgtgtacggc
     3661 gcatagccgc cgtacatctt gaggtcgtat ggacattgct tgtgactcac gcttttgaga
     3721 tagctcaccg cccctgaaga aagtcccatc gccagtatca gaaagaggaa aacacggcgc
     3781 aggggggcga gcgaagcaat gcggaacgaa acaagataag ctgccagcac cgccacaccg
     3841 agaatcacca ctgcatattt ggcccaccgg tgcatcacca cttcgagaaa ccagcggttg
     3901 cgcaatggaa actgttgggt taccgggtcg aaaaataggc gcgtgatgcg ctcgtcaaca
     3961 cccagtaggt aaatcaccca taggacgagg ccagccgcaa tcaggggtat gaccagatgc
     4021 aaaaagtaaa aacgcgaagc gctgtcttgt ccttgcgtct gcgctgattt cactgttgtg
     4081 ctttacggaa gcgccagcct aatgcaagcc gtagcggcgg tggatataaa aatcgtaggc
     4141 cgactggaac acactcgccg catttttcat ttgttcctgg tcaagcgcag tcttgttctg
     4201 gcgattggtc ttcttgtcgt agagataagt gccgctgatt ttgcggaaat tgagctcggt
     4261 gagcttggtg ccatcataca gggcgatgtc gcggttgtgg ttgagcagca caaaccggtc
     4321 ttttgggtcg cttttaaaca catccttgcc gaaaaacacg ctgtcatagg aaatattgag
     4381 catgccgagc accgtgggcg cgatatccat ctggctcgtt agagtatcca ttttgtacgg
     4441 cttgaaatgc ccggctgcgt aaatcaatag cggcagctcg tagctgcgca agggaatatc
     4501 ttctctcccg tagacccgcg cgccgtggtc gccgacgatg atgaccaaag tgtcattgaa
     4561 atacggcctg tttttgattg catcgaagaa cttgccgatg gcgtagtcgg catagcgcac
     4621 gccggcctcg cggccgccgc ctttcgcggg cacgccgggt acgccttccg ggaaggtgaa
     4681 gggtttatgg ttcgacgtgg tcatcacgat ggagaatatt ttctcgccgc gcgcgtgctg
     4741 cgtgtcgaaa atgcgcaggg cattgcggaa caggtcttcg tcggacacgc cccagatatt
     4801 gctgaatttc ggagtgtcca tgtcggtgcg gtccaccaca cggtagccgt tattgccgaa
     4861 aaagtagttc atgttgtcaa aagtgccata gccgccgtag atgaaagtcg gcgcgtagcc
     4921 gtttttcttc atcaccgtcg accagttgaa catgccttcg ttgttggggc gtttcacgat
     4981 cgcctcggcc ggcaccggcg ggaaggaagc ggacactgct tccatgccgc gcacggtgcg
     5041 cgtgccggta gcgtagacgt tggtgaacac cacgctgtcc ttggcgacgc ggtcgatgtt
     5101 gggggtgaga ccgcgcgtgt cgccgtaggc gccgacgaaa tcggcgccaa gagattcttc
     5161 aagcagaatc acgacattga gcagcagggg ttttccggga ttctcaatgt gccgcgcgat
     5221 ggggttgttg gagccggaga taaaggttga gttggattgc gccaccagct ggcgcaagcg
     5281 gcgccccgct tcctcggcgg aaacggtagt gtagaaattt gggtaatcca tttcgctgtt
     5341 catcgcggcg ttgaagaacg aataaatgcc gtttgccgtg agttcgttgg cgacgcggtt
     5401 ctccgaatag cggccggtat tgatgttgac ggtggcgtgc acaatcagga gcagaatcat
     5461 ccatgtgccg aaaggcttca cgcgcgcagc tgaccgctct tccttcagca tgctgcggtt
     5521 gagcatgggg taaaatcgcc acatcacggc gaaggtggcc agggcggtgg caatcagcgc
     5581 ccaccccacc gggtaggatt cccagatgtt gacgaacact tcatgcggga acactaggta
     5641 ttcgacggcg acgaaattaa agcgcgagtt gaactcgtca aaaaagtagt attccaccgc
     5701 gccgagataa atgaggccgt acagcgaagc ggcaaatgcg gtgatcagaa aaacgcgctg
     5761 tccaatcgag cggaacgctt tttgcgggaa cagaaacaga tacagcgcaa aaggcgcgaa
     5821 aaggtaaagg caggtgatga ggtcgtacaa cgtgccgatg ggcaggatgg cggcgagaga
     5881 accgaccgcg atttggcctt ccttgatcgg gcgcaccgcc agaacaatgc gggtgaggaa
     5941 agaaatgatg agatacagtg tggcggctaa atatagaatg ccaaaccggc tggattcata
     6001 aatccgccgc aacaaaccgc cggaagagga aatgattgac ctcaacgccg ggttggcgcc
     6061 ggcagtgatt gccagcggtc gtttatcaac ccctcaatcg gctggaagtt tgccttgtac
     6121 gccatcttgc ggctttcccc gatccagtag ccgagataga gatactgcag cccgagattc
     6181 ctgcacagtt cgatttgcca catgatgttg taggtgccga ggctcgcgcc cggaagattg
     6241 ggctcgaaga aggtgtagac cgaggacagg ccgtcgtgca ggcggtcgat gatgctcacc
     6301 atgcgtaaat cgcgcccttc gcggaattcc accagctgcg aatccacatt gctctgtagc
     6361 aggaaatgct ggtattgctc gcggctatcc tggtccatgc cgccgccgga atgacgcttg
     6421 gcctgatagc gcagatacag cgcgtaatgc tcgggatggt atttaagctc cagcatttcc
     6481 gcgcgcagat tctggtggcg cttccaggtg cggcgctgcg tgcgcgtggg gcaataagcg
     6541 ccggcgatga ggcgcaccgg cacgcaagcg cggcagctgt cgcaatgcgg acggtaggta
     6601 aacgcaccgc tgcggcggaa gccggttttc accagctcgc tgtagaccga ggtgtcaatc
     6661 aggtggctgg gggtggcgac ctgcgagcgc gccatttttc ccgtcagata gctacacggg
     6721 tagggggctg tagcgtagaa ctgcagcacc gacagcggga attcattcaa ataagtcatg
     6781 gtcgagacgc cattttcctt gggtacccgc atagtttatc aattcttcca gcgtccgcgt
     6841 aaattgcttg cgcgggatct cgcgcgcgcc gaacgaagcc agatgcgcgg ttttcatctg
     6901 gcaatcaatc aggccaaagc cccagcggct taactgcctc accaaatgga caaaagcgat
     6961 tttggaagcg tcggtccggc gcgcgaacat ggattccccg aaaaacacgc ggcccagtgc
     7021 cacgccatag agtccccccg ccagtttgcc gccgatccgg gtttccagcg aatgcgcgag
     7081 gcccactcgg tgcaattcct gataagcttg catcatgtca ggcgtaatcc acgttccggg
     7141 ctggccgcgg cggggagccg cgcagctttt catcacctcg gcaaaggcgg tgtcggcgcg
     7201 gatttcataa tcgcgtttct tcaacacctt gcgcaatgag cgcgaaatct tgagttcggc
     7261 ggggaaaagc accattcgcg gatcggggct ccaccacagt atcggctcgc cttcgttgaa
     7321 ccagggaaaa atcccttttg aataagcttc gaccaggcgc ttgagggaca aatcgccgcc
     7381 gccggcgagc aggccattcg gctcatgcaa tgcctgttcg gacgggggaa acggtgtgtt
     7441 gcgccctagc cagcaaatca tctttaaagg ttcccttatt ttacccgatg tcccgcagtt
     7501 gcaaaaaaac agcgcgcctg aaagcgcgct gggggttatg caggcgcgta tcaataaccg
     7561 gaacgcggcg cttcgttgag cacaatgacg ccgcatgcta tacgcccccc ggagtttccg
     7621 gcgggcgggg tatagtcatc gcggttggcg tggacgatca cgctcctgcc gacgatgctg
     7681 ttagcggcgg atttgtcgag cgtgatgccc ggcgcttcgt aggagtaagc ggcattaccg
     7741 gagctatcgg cattcaggtt gccaaggtcg ccggcatggt gatcggcgct gtccggtccg
     7801 ccatgttgtt tgccctgggg attaaaatgc cctccggcgc tcatgccatc ggccgcgctg
     7861 caatcgcctt tttcgtgaac atgaaagccg tgcatgcccg gcttgagacc gtttacctta
     7921 gccaccacca acaccttgtc gcctttctgg gtaaaggtga cggtgccccc gactttgttt
     7981 cctttggtgg gctgtagatt ggcggtggcg gttttgccgg acagccagcc gggcatccag
     8041 gcacaccccg ccaccatcag tgagcaggat aaagctgcaa tcaaaattct catttcatct
     8101 cctttgctgt ttaggtatta aatgccgggg ggcgttatct agcacctgac ttgctgttgg
     8161 caaaagtttg tcaactcggt gatgatgacg tcgctggtaa ccggtttctg cgctttgttg
     8221 gagccgtact ccagggacag cctgaagtcg tacttggaaa agaaatcgtt aaatagttcc
     8281 ccgcccaggg tgccgaccgc gaataattca tcggtctttg cgtcgtattc gaggatgatc
     8341 gccgccagcg gctgcggcgc cgggccggaa acgactttgg cgccctgcgc ggggtcgtat
     8401 tcgctgtgct cggcgcagca gtgaatcaca ttgatgtgcc cgacgctggc ctccgtttta
     8461 tcggcacggt agctgatgaa actgatttgc ttggtgggat aggcgagccg gtgcgcgcaa
     8521 atcgccgagt aggccacaat cgagctaccg gccccgacgc cgcccctcca gtggtaattc
     8581 ctgccgtccg cggtggcgag cagcgcatcc gttgcagtgg gtttgccgag attcaagagg
     8641 aaacaaggcg tgccttgaaa cggatagtgg aaaatatagt ttcgcttgat ttgcagcctg
     8701 gatgcctgga gcgggcggcc gtcgtaatcc accagccggg cgcgggcata gaatcggggc
     8761 tcggtgtttt gcgcagcgaa gccgctgttg accgccgcca cggccgcgcc cgcggcgcag
     8821 attttgatga attcgcgtct ttccataccc actcccgggc agagctgttt atgtaacact
     8881 catcgcgccg caatgtcaac cgagcaggtc atggtttaga acttacaaaa aaaagacggc
     8941 tgcaaaccgc tgccgatttg accaataaca ttttcatcac gggaaatcgc ctgagcaggt
     9001 gctggcaatg cgctccggtt cgggctattc ttggtttatg aataatcttg aggagaaaaa
     9061 atgcccgaat taagcccgga agttgcaggc ctgctcaagg aattgcgcac ctttctcgcc
     9121 aatggcagcg agccgtggga acagcggcgc gaactcatgc tcaagatcaa caatctggtg
     9181 gaaggcgggg aaactgattt gttcgtcggc atggcgcggg agatgagccg catcggcgcc
     9241 acttatacca acctgttgca ggcttttgcc cagttcggcg ccgcgcagca aggcggggcg
     9301 aatctcgcgg attggttcat gcgttcgttt cagcagccgc aggccatggt cccgccgttc
     9361 atgtcgccgg cggcggggtt cgattacttc aagcagcttg ccgatttcat gagcgctcaa
     9421 gccaaatcgg caggcgcctc gcaaaagaaa aaagattaat caagcgttta acggtagaac
     9481 aaatcgtgca aatgagccgc gacggtatgc ctgcggaaac gatcgtaaag cagttgcgcg
     9541 actcgcgcga ggtgtaccgc ctaagcgctt cgcagcttgt gaaactgcat gaccagggcg
     9601 tggcggacga ggtgatcgac tacatgtaca tgcaggcaac ctacattcag gcggagcgag
     9661 gtgaggaaat acagtaccag agccagttct gtggctacgg cttgtattgc cggccgtttc
     9721 cttacttacc gccggcgtta atgcgcatgt gctgaaccga cgacaggcaa aaaaatgggc
     9781 gtggagaaca cgcccaaaag gcttaataaa ggagaatgag caagcagaaa cactatgctt
     9841 gcgaaaatac aaaccagcaa gaacggtgcc agcaatccat tctcggccaa tcaatgagtt
     9901 atgagatgaa gcgggctggc cgtttgtgtc ggaagcgacg caggtgttgc aaactatcca
     9961 ccgattttgg cacgatggca cgccaaagag gataccgggc attggtcaga gtgcttgttg
    10021 ctgccgtgag tggctcccca agcagggagg aaaagtgggc actggttgga gctgattcgt
    10081 tttcgctaac gcttaagctc agccgcgcgg taacgcgtca gctggagtg
//
LOCUS       DATIOW010000131         3708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_265146, whole genome shotgun sequence.
ACCESSION   DATIOW010000131 DATIOW010000000
VERSION     DATIOW010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265146"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VLL03_04295"
     CDS             complement(<1..334)
                     /locus_tag="VLL03_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit N"
                     /protein_id="HSS46620.1"
                     /translation="MNFVVPDLTPAYPEIFLLAMVSLILVIDLCISDERRFITYHLTQ
                     LTLLGCAVVTVAVSEPGIRYSFSRMFVADNLADVLKIMVYISVCVMLIYSRTYIYLRG
                     MFRGEFFAL"
     gene            complement(440..1924)
                     /locus_tag="VLL03_04300"
     CDS             complement(440..1924)
                     /locus_tag="VLL03_04300"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HSS46621.1"
                     /translation="MLLGFPLLSLAIWLPILFGLLVLATDGDRNANVARWIALAGALL
                     GFLVTLPLYAKFDLPVAGMQFVELRPWIARFNVHYHLGVDGISVLFILLNSFMTLLVV
                     ISAWRVIESRVAQYMAAFLIMAGLVNGVFSALDAVLFYVFFEAMLIPMFLIIGVWGGP
                     NRVYAAVKFFLYTLLGSLLMLVAFIYLYNASEGSFSILDYHQLPLSLTVQILLFIAFF
                     LSFAVKVPMWPVHTWLPDAHVEAPTGGSVILAAILLKIGGYGFLRFSLPIAPDASHYL
                     AGFVITLSLIAVVYIGLVALVQADLKKLIAYSSISHMGFVTLGIFIFNAYGAEGALVQ
                     MLSHGFISGALFLCVGVLYDRMHSRLIADYGGVVNKMPVFASLFMLFALANSGLPATS
                     GFVGEFMVIMGSLKVNVWYAALAATTLVFGAAYTLWMYKRVIFGAVANRHVEALADID
                     LREALFLGLLALAVLGMGVYPKPFTEVMHASVGNLLAHVAQSKL"
     gene            complement(1948..>3708)
                     /gene="nuoL"
                     /locus_tag="VLL03_04305"
     CDS             complement(1948..>3708)
                     /gene="nuoL"
                     /locus_tag="VLL03_04305"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HSS46622.1"
                     /translation="IYEWMNIGGLKMEVGFLVDGLTAMMMCVVTFVSLMVHIYTIGYM
                     HDDPGYQRFFSYTALFTFSMLMLVMANNFLQLFFGWEAVGLVSYFLIGFWFTRESAIF
                     AGLKAFLVNRVGDFGFLLGIALILAYFGTLDYAEVFKHAPQLANRTIEILPNEPWVVM
                     TLICILLFTGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGIFMVARMSPLY
                     ELSETALSVVLVIGAITALFMAFIAIVQNDIKRVVAYSTLSQLGYMTVALGASAYAAS
                     IFHLMTHAFFKALLFLCAGSVIIALHHEQDMRKMGGLKKYMPITYWTAVVGSLALVGI
                     PPFAGFFSKDALIEAVGASHIPGSGIAYFAVTLGTFITALYSFRLLFMTFHGEPRMDG
                     HAREHLHESPWVVTVPLILLAIPSVYAGWQYIEPMLFGGHFGSAIVALPEHDVLGYLK
                     EHYHGVPAFILHGFTALPFWLALAGIGVAYYLYILRTDLPAIIAERFSAIYTLLDRKY
                     YFDELYQWLFAGGARGVGGGLWQVGDVTVIDGFFVNGTAKVVRLASGVIRRLQSGYIY
                     HYAFTMIIGIFVLLMWFARP"
BASE COUNT         1006 a         1076 c         1040 g          586 t
ORIGIN      
        1 ccagcgcgaa aaactcgccg cggaacatgc cgcgcaaata tatgtaggta cgcgaataga
       61 taagcattac acacaccgag atatacacca tgatcttgag cacgtcagcc aggttatcgg
      121 cgacaaacat gcggctgaag ctgtagcgga tacccggctc gctcacggca accgtgacca
      181 cggcgcagcc caacagggta agctgagtca aatgataagt gatgaaacgc cgctcatcgc
      241 tgatgcagag gtcaatcacc agaatcagcg acaccatcgc caagagaaat atctccgggt
      301 aggcgggcgt cagatcaggc acaacaaaat tcatgctaaa cctttcaccg cagagggcac
      361 agaagaaaac aatgcaaatt caaaaataac agaatttctc cgcgctttca gcgttctctg
      421 cggttaatga atcccaaatt cataacttgc tctgcgccac gtgcgcgagc aggttgccga
      481 ccgaggcgtg catcacttcg gtgaagggct tgggataaac gcccattccc agcaccgcca
      541 gcgccagcaa acccagaaac aaagcctcgc gcaagtcaat gtcggcaagc gcctcgacat
      601 ggcggttcgc caccgcaccg aatatcaccc gcttgtacat ccatagcgtg taggcggcgc
      661 cgaacaccag cgtggtcgcg gccagcgccg cataccagac attgaccttg agcgaaccca
      721 tgatgaccat gaattcgccg acgaaaccgc tggtcgccgg caacccggaa ttggccaagg
      781 cgaacagcat gaataaagac gcaaataccg gcatcttgtt cactaccccg ccgtagtcgg
      841 caatcaggcg cgaatgcatg cggtcgtaga gcacgccgac gcacaggaac agcgcgccgg
      901 agatgaagcc gtgcgagagc atctgcacca gcgcgccttc cgcgccatag gcattgaaga
      961 tgaaaatgcc cagtgtgaca aagcccatgt gcgaaatcga ggaataggcg atgagttttt
     1021 tcaagtccgc ctgcaccagc gccaccagtc cgatgtacac cacggcaatc agcgaaagcg
     1081 taatcacgaa gccggcgaga tagtggctgg catccggggc aatcggcagc gagaaacgca
     1141 ggaagccgta gccgccgatt ttcagcagga tggcggcgag aattaccgag ccgccggtcg
     1201 gcgcttcgac gtgcgcatcc ggcagccagg tatgcaccgg ccacatcggc accttcaccg
     1261 cgaacgacag gaaaaacgcg atgaagagca ggatttgcac ggtgagcgaa agcggcaatt
     1321 ggtgatagtc aagaatggaa aagctgccct cggaggcgtt gtagagataa atgaacgcca
     1381 ccagcatcaa caatgaaccg agcaaggtgt acaggaaaaa cttgacggcc gcgtaaaccc
     1441 ggttcggccc gccccacacg ccgatgatga ggaacatcgg aatcagcatc gcctcgaaga
     1501 acacgtagaa cagcaccgca tccagcgcgg agaacacgcc gttcaccagc ccggccatga
     1561 tgaggaacgc cgccatgtat tgcgcgaccc ggctctcgat cacccgccag gcggagatca
     1621 cgaccagcag cgtcatgaag ctattcaaga ggatgaacag cactgaaatg ccatccacgc
     1681 cgagatgata atgcacgttg aagcgcgcga tccagggacg cagttcgaca aactgcatgc
     1741 cggctaccgg gagatcgaat ttggcataga gcggaagcgt gacgagaaaa cccaaaagtg
     1801 cgccggcgag cgcaatccag cgggccacat tcgcattgcg gtcgccgtcg gtggcgagca
     1861 ccaggagccc gaacagaatc ggcagccaga tggcaaggct tagcagcggg aaacctaata
     1921 gcatctagtg tccattctgc cggttaatca gggccgcgcg aaccacatca agagcacgaa
     1981 aatgccgatg atcatggtga aggcataatg gtaaatgtag cccgattgca ggcggcgaat
     2041 cacgccggaa gccagccgca ccactttcgc cgtcccgttc acgaagaaac cgtcgatcac
     2101 cgtgacatcg ccgacttgcc acaagccgcc gcccacgccg cgcgcgccgc cggcaaacaa
     2161 ccattggtaa agctcgtcga agtagtattt cctgtccagc agagtgtaaa ttgcgctgaa
     2221 gcgctcggcg attatcgcgg gcaaatcggt gcgcaggata tagaggtaat acgccacgcc
     2281 tataccggca agcgccagcc agaacggcaa ggccgtgaag ccatgaagaa taaaggcggg
     2341 cacaccgtga taatgttcct tcaaatatcc cagaacatcg tgctcgggca gcgccacaat
     2401 ggcgcttccg aaatgcccgc cgaacagcat gggctcgatg tattgccagc cggcataaac
     2461 cgaagggatg gccagcaaaa tcagcggtac cgtgaccacc cagggcgatt catgcagatg
     2521 ctcgcgcgca tgcccgtcca tgcgtggctc gccgtggaag gtcatgaaca gcaggcggaa
     2581 cgaatacagc gcggtgatga acgtgccgag ggtgacggca aaataagcga tacccgagcc
     2641 cgggatgtgc gaggcgccaa ccgcttcgat gagagcatcc ttggagaaaa atccggcaaa
     2701 aggcgggatg ccgaccagcg ccagtgagcc gacgaccgca gtccagtagg taatcggcat
     2761 gtatttcttc aagcccccca tcttgcgcat gtcctgctcg tgatgcaggg caatgatgac
     2821 cgagcccgca cagaggaata gcagcgcttt gaagaacgca tgcgtcatca gatggaaaat
     2881 gcttgcagcg taggcggaag cgcccagcgc tacggtcatg taacccaatt gcgagagcgt
     2941 ggagtaagcc accacgcgct tgatgtcgtt ttgcacgatt gcaatgaacg ccatgaacag
     3001 cgcggtgatc gcgccaatga ccaagaccac ggacagcgcg gtttccgaca gctcatacag
     3061 cggcgacatc cgcgccacca tgaagattcc ggcggtgacc atggtcgcgg catgaatcaa
     3121 ggcggaaatc ggcgtgggcc cttccatcga atccggcagc cagacatgca gcgggaattg
     3181 cgcggatttg cccatcgcgc ccgtgaacag cagaatgcaa atcaacgtca tcaccaccca
     3241 cggttcattc ggcaggattt caatcgtccg gttcgcgagc tgcggcgcgt gcttgaacac
     3301 ctcggcataa tcgagcgtgc cgaaataagc gagtatcaac gcaataccga gcaaaaagcc
     3361 gaaatcaccg acgcgattga ccaaaaatgc ctttagaccg gcaaaaatcg cgctttcgcg
     3421 tgtgaaccag aagccgatga ggaaatagga aaccagcccc accgcttccc agccgaaaaa
     3481 cagctgcagg aagttgttgg ccattaccag catcagcatg gaaaacgtaa acagcgcggt
     3541 atagctgaaa aagcgctgat agccgggatc gtcatgcatg tagccgatgg tgtagatgtg
     3601 caccatcagc gagacgaagg tgacgacgca catcatcatc gcggtcaggc cgtcgaccag
     3661 gaagccgacc tccatcttca ggccgccgat gttcatccac tcgtagat
//
LOCUS       DATIOW010000132         4856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1991796, whole genome shotgun sequence.
ACCESSION   DATIOW010000132 DATIOW010000000
VERSION     DATIOW010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1991796"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /gene="pabC"
                     /locus_tag="VLL03_04310"
     CDS             complement(<1..617)
                     /gene="pabC"
                     /locus_tag="VLL03_04310"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR03461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HSS46623.1"
                     /translation="MILVNGVRTDSLSVHDRGLMYGDGVFRTLRVSHGVALCWQRHYR
                     KLLADCTALNINCPSIAVLSTEVEAVAKANPECVVKIIITRGVGTRGYRVAPGATPNR
                     VVMSSPLSDYPASHYEQGVKVRICDLRLGLQPRLAGIKHLNRLENVLARQEWNDEAIA
                     EGLLLDGEGHVICGTMTNLFTLKGAELCTPDLSRCGLAGVQRERVL"
     gene            complement(704..1942)
                     /gene="fabF"
                     /locus_tag="VLL03_04315"
     CDS             complement(704..1942)
                     /gene="fabF"
                     /locus_tag="VLL03_04315"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HSS46624.1"
                     /translation="MSRRRVVITGLGIVSPVGIGIAEAWQNIVAGEPGIARITRFDAS
                     GFASQIAGEVKGFNPENYLSAKDARRMDMFIHYGMAAAIEAIKDSGIEITPQNAERIG
                     VNIGSGIGGLPVIEETHNAFLQGGPRKISPFFIPMTIINMISGNLSILYGLKGPNLAM
                     VTACTTATHCIGDSGRLIEYGDADMMVAGGSESTVTPLARGGFSSARALSTRNDDPAT
                     ASRPWDVGRDGFVLGEGAGVLVLEEMEHAQKRGARIYAELAGFGMSADAHHMTAPCED
                     GEGAARCMVNALRNARLNPDQVDYVNAHGTSTPLGDIAETVAVKRCFGDHAKKIAISS
                     TKSMTGHLLGAAGGVEAIFSALSVHHQVAPPTINLFKQDPQCDLDYVPNTARNMKIRA
                     ALSNSFGFGGTNGTLVFTKA"
     gene            complement(1981..2217)
                     /gene="acpP"
                     /locus_tag="VLL03_04320"
     CDS             complement(1981..2217)
                     /gene="acpP"
                     /locus_tag="VLL03_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012735022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HSS46625.1"
                     /translation="MENIEQRVKKIVAEQLGVNEGEVKNESSFVDDLGADSLDTVELV
                     MALEEEFECEIPDEEAEKISTVQQAIDYIKTHLN"
     gene            complement(2328..3071)
                     /gene="fabG"
                     /locus_tag="VLL03_04325"
     CDS             complement(2328..3071)
                     /gene="fabG"
                     /locus_tag="VLL03_04325"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HSS46626.1"
                     /translation="MLENQIALVTGASRGIGQGIALELGKQGATVIGTATTDNGALAI
                     SAYLAKAGINGAGMALNVNDGEAIERLLEDLQNRHGAISILVNNAGITRDNLLLRMKD
                     EEWDDIMSTNLKSVFRLCRAVLRPMIKARYGRIINITSISGEIGNPGQANYAAAKAGI
                     AGFSKSLAREVASRNITVNCVAPGFIDTDMTRALNEARRQALLQQHVPMGRLGRVEDV
                     AVAVAFLASNGAGYITGCTLQVNGGMYMN"
     gene            complement(3089..4018)
                     /gene="fabD"
                     /locus_tag="VLL03_04330"
     CDS             complement(3089..4018)
                     /gene="fabD"
                     /locus_tag="VLL03_04330"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006759768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HSS46627.1"
                     /translation="MKFAFVFPGQGSQSVGMMRGFADLPVVRQTFDEASGILNQDLWT
                     LVKNGPETALSQTVNTQPVMLIAGIAVYRAWRKLGGAAPRLLAGHSLAEYTALVAGGA
                     LDFAEALPLVRFRAETMQQAVPEGTGGIAAILGLNEIKIKEVCLEAAQGEVLEAVNFN
                     SPDQIVVAGHRTAVLRGMELAKTKGAKRAIMLPMSAPSHCSLMRGAALKLKERLQSAA
                     IKPPQIPIVHNVDVSEHAEPDAIKEALVRQLYNPVRWVETIQVLAQRDVTHVAECAPG
                     GVLAGLNKRIAPAQEVFAFNDGAGLKQALAVLA"
     gene            complement(4032..>4856)
                     /locus_tag="VLL03_04335"
     CDS             complement(4032..>4856)
                     /locus_tag="VLL03_04335"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HSS46628.1"
                     /translation="AAENEYASDLALNASRIALQTAAVKTDDIDLIIVATTTPDMIFP
                     STACILQSKLGVKNCRAFDVQAVCSGFVYGLATADLFIRSGKSHCALVVGVEIYSRIL
                     NWNDRATCVLFGDGAGAVIVKAGEAPGILSTHLHADGSYYKALAVPGSVCDGKVTGNP
                     YVVMEGNAVFKFAVKVMEDMVDEALAANKLRKSDIQWLIPHQANIRIIQAAAKKLGLD
                     MDRVVVTVDRHANTSAASIPLALDDAVQDGRIRRGELILMEGVGGGFTWGSVLLRW"
BASE COUNT         1013 a         1485 c         1287 g         1071 t
ORIGIN      
        1 agaacgcgct cgcgctgcac tccggccagc ccgcaacggg acagatccgg tgtgcatagc
       61 tcggcgcctt tcagcgtgaa caggttggtc atggtaccgc agatgacatg gccttcgcca
      121 tcaagcagca gtccttcggc aatggcttcg tcgttccatt cctggcgcgc caatacgttt
      181 tccagccggt tgagatgctt gatgccggca agccgcggtt gcagtcccaa tcttaaatca
      241 cagatccgca ccttcacccc ctgctcgtag tgacttgccg gatagtcgga aagcgggctc
      301 gacataacga cgcggttggg tgtggcacca ggcgcgacac gataaccccg cgttccgaca
      361 ccacgggtga tgatgatttt gaccacgcat tcaggattgg cttttgcgac cgcttccact
      421 tccgtgctta aaaccgcgat ggaagggcaa ttaatgttga gcgccgtaca gtccgccagc
      481 agtttgcggt aatggcgctg ccagcacagc gctacgccgt ggcttacacg cagggtgcgg
      541 aacacgccgt caccgtacat caagccgcgg tcatgcacgc ttaacgaatc ggttcgaaca
      601 ccgttgacca aaatcatttt aagttgaccg gcagcacgca aaaagccgta atagatgaag
      661 ccaatttaac ccagctgccg aaaaaggaaa aggcgccgcc gtctcaagcc ttggtaaaaa
      721 ccagcgtgcc gttggtgccg ccgaaaccaa acgaattgga caaggccgcc ctgattttca
      781 tgttgcgcgc ggtgttcggc acgtaatcga gatcacactg cggatcctgc ttgaagaggt
      841 tgatggtggg aggggcgacc tggtgatgga cgctcaacgc tgaaaatatc gcttccaccc
      901 cgcccgccgc gcccagcagg tgcccggtca tggatttagt ggaactgatt gcgatttttt
      961 ttgcgtgatc gccgaaacag cgcttaaccg ccactgtttc ggcaatatcg cccagcggcg
     1021 ttgaagtgcc gtgggcgttg acgtaatcca cctggtccgg gttgagtcta gcgttgcgca
     1081 aggcattcac catgcagcgc gccgcgcctt cgccgtcttc gcagggcgca gtcatgtggt
     1141 gagcgtcggc gctcatacca aaaccggcaa gctcggcgta aatacgcgca ccgcgttttt
     1201 gggcatgctc catttcctca agcaccagca cccccgcccc ttcaccgagc acaaaaccgt
     1261 cacgccccac gtcccacggc cgactggcgg tggccggatc gtcgttgcgc gtggacagcg
     1321 cacgggcgga agagaagccg cctctggcca gcggtgtcac cgtggattcg gagccgccgg
     1381 ccaccatcat gtccgcgtcg ccgtattcga taagccgccc cgaatcgccg atgcaatgcg
     1441 tggccgtggt gcaggcggtg accatcgcca gattcgggcc tttcaatcca tacaggatgg
     1501 acaggttgcc cgaaatcata ttgatgatgg tcatggggat gaagaatggc gaaatcttgc
     1561 gcggcccgcc ctggaggaag gcgttgtggg tttcctcgat gaccggcaag cccccgatac
     1621 ccgacccgat attaacgccg atgcgctcgg cattctgcgg cgtgatttca attcccgaat
     1681 ccttgattgc ctcgatagcc gcggccatcc cgtagtgaat gaacatatcc atgcggcggg
     1741 catctttcgc cgaaagatag ttctcgggat tgaagccttt tacctcgccg gcaatctgcg
     1801 aagcaaaccc tgaagcgtcg aagcgggtga tgcgggcgat acccggttcc cccgctacga
     1861 tgttttgcca cgcttcagcg atgccaatac cgaccggcga cacgataccg aggccggtaa
     1921 tgacgactct gcgtctggac aagattactc caggtgccaa gggttaaaac gcggggggaa
     1981 tcagtttagg tgggttttga tatagtcaat ggcttgctgc acggtggaga ttttttccgc
     2041 ctcttcgtcg ggaatctcgc actcaaactc ctcttccagc gccatcacca gctcaactgt
     2101 gtccagcgaa tccgcgccca gatcgtccac aaacgaagat tcgttcttga cttcgccctc
     2161 gttgacgccg agttgttcag caacaatctt ttttacgcgt tgctctatgt tttccatcta
     2221 aatccctttc cggttccgtg gtggtcaaag caattcaatc tcgggctgca gcaaattcgg
     2281 ctattctacc aaatttaccc tgattacaaa atcgcaatgg tcgctaccta attcatgtac
     2341 atcccgccgt tcacctgcag ggtgcagccg gtaatgtaac cggcgccatt tgaagccagg
     2401 aaagccactg ccactgcaac gtcttcgacc cgccccagtc gccccatggg cacatgctgc
     2461 tgcagcaacg cctgtcgtcg cgcttcgttg agcgcccggg tcatgtcggt gtcgataaag
     2521 ccgggcgcga cgcaattaac cgtgatattg cgactcgcta cttcgcgtgc cagtgacttg
     2581 ctgaatcctg cgattccggc cttggccgcg gcgtaattgg cttgaccggg attgccgatt
     2641 tccccggaaa tggaagtaat gttgataatg cggccgtagc gtgcttttat catggggcgt
     2701 aatactgcgc gacacaacct gaataccgat ttcaggttgg tcgacatgat atcgtcccac
     2761 tcctcgtcct tcatgcgcag cagtagattg tcacgggtaa tgccggcatt attgactaga
     2821 atcgaaatcg cgccatggcg gttctggagg tcctcgagca ggcgttcaat cgcctcaccg
     2881 tcattgacat tgagcgccat tccggcgcca ttgatccccg ccttcgccag ataggcgctg
     2941 atggccagcg cgccgttatc cgtggtcgcg gtgccaatca cggttgcgcc ttgtttgccc
     3001 agttccaaag caatgccctg cccgatcccg cggcttgcac ctgtgactaa agcaatttgg
     3061 ttttccagca tttttagtcg tgaaaaagtc aggccagtac cgccaacgcc tgcttgagtc
     3121 ccgccccatc gttgaacgca aatacttcct gagcgggagc aattcgcttg ttgaggccag
     3181 ccaacactcc gcccggcgcg cattccgcga catgcgttac gtctctttgc gccaaaacct
     3241 ggatggtttc cacccagcgc actggattat acagttgtcg taccagcgct tccttgatcg
     3301 cgtcgggctc cgcatgttcg ctcacatcca cattatgcac tatagggatt tggggtggct
     3361 tgattgccgc tgattgcaaa cgttctttca gctttagcgc cgctccgcgc atcagcgaac
     3421 aatgcgaagg cgcgctcatc ggcagcatga tggcgcgctt tgcgcctttg gtcttggcca
     3481 gttccatgcc gcgcagcacc gctgttctat gcccggcgac cacaatctgg tccggcgaat
     3541 tgaaattcac cgcctccagg acttcgccct gagccgcttc caagcagact tccttaatct
     3601 ttatctcatt caggccgagt attgcagcga ttccccccgt tccctcgggt acggcttgct
     3661 gcatagtttc tgctcggaat cgcaccagcg gcaacgcttc ggcgaaatcc agcgctccgc
     3721 ccgcgaccaa ggcagtatat tctgccaagc tgtgaccggc cagcaagcgg ggcgccgcgc
     3781 cgcccagctt tcgccaggcg cgataaactg cgatgccggc aatcagcatc accggttggg
     3841 tattgactgt ctggctcaaa gccgtttccg gaccgttttt caccagcgtc cacaaatcct
     3901 gattaagaat gcctgaagcc tcatcaaagg tctggcgtac cacgggcaaa tcggcaaagc
     3961 cgcgcatcat gccaaccgac tgcgagcctt ggccggggaa aacgaatgcg aatttcatgt
     4021 tttcttattc actaccagcg caacaggacc gagccccaag tgaatccccc gcccacgcct
     4081 tccatgagaa tcagctcgcc acgcctgata cggccatcct gcaccgcgtc atccaaagcc
     4141 agtgggatcg aggcagcgga agtgttggca tggcggtcca ccgtcactac cacccgatcc
     4201 atgtctaaac ccagtttctt ggcggcggcc tgaatgatgc ggatattggc ttggtgcgga
     4261 atcagccact ggatatcgct ttttcgcaac ttgttggcgg cgagggcctc atccaccata
     4321 tcttccatta ccttcactgc aaatttgaac accgcattgc cttccattac cacatacgga
     4381 ttgcccgtca cctttccgtc gcacacactg cccggaacag ccaaggcttt gtagtagctg
     4441 ccgtctgcat gcaaatgagt ggagaggatt cccggcgctt cgccggcctt tacgataacc
     4501 gcgcccgcgc catcgccgaa cagcacgcaa gtagcgcggt cgttccagtt caagatgcgc
     4561 gaataaattt caacccccac gaccaaagca cagtggctct tgcccgaacg aataaacagg
     4621 tccgcagtgg ccaagccata aacaaaaccg ctgcacactg cttgcacatc gaaagcccgg
     4681 caattcttta caccgagctt ggactgcagg atgcaggcgg tactgggaaa aatcatgtcc
     4741 ggcgtggtgg tagcgacgat aatcaagtcg atatcgtccg tcttcacggc cgccgtttgc
     4801 agcgctatcc tgctcgcgtt aagcgccaag tcgctcgcat attcattttc tgctgc
//
LOCUS       DATIOW010000133         3476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1180113, whole genome shotgun sequence.
ACCESSION   DATIOW010000133 DATIOW010000000
VERSION     DATIOW010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180113"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /locus_tag="VLL03_04340"
     CDS             <1..171
                     /locus_tag="VLL03_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HSS46629.1"
                     /translation="FARLYHRDGKEGYLKDLPLVMQYLRKACERYSELAPLLKLLEEL
                     EGKTQIKTGYTF"
     gene            272..976
                     /locus_tag="VLL03_04345"
     CDS             272..976
                     /locus_tag="VLL03_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HSS46630.1"
                     /translation="MRAMILAAGHGERMRPLSDTTPKLLLPVGDKPLIAWILENLAAA
                     GFHEIVINHSHFGKQIESALGDGAGIGVRIVYSPERQALETAGGIAQALPLLGAQPFL
                     VVNGDIYCDLDFRQLLPQLNRMREKPDSDRAYLVLVDNPGHHPEGDFALEQNRVTLSE
                     TQKLTFSGIGLYHPALFAGVPQGGKMKLAPLLLDQIAAGKVGGEHYHGLWRDVGTPER
                     LRELDELLKSQPARGK"
     gene            1001..2311
                     /gene="pepP"
                     /locus_tag="VLL03_04350"
     CDS             1001..2311
                     /gene="pepP"
                     /locus_tag="VLL03_04350"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HSS46631.1"
                     /translation="MDDYSIFAQRRARLIKKMQAGVALVPTAAERVRNRDSHYPYRFD
                     SYFYYLSGFTEPGAVLVLVAGKQPKSVLFCRDKDPEREIWDGRRYGPPAARKRFRFDE
                     GCSLNQLDEMIPQLLANQPVLYYAMGTDEAWDARMLGWLNQVRAQTRAGVTAPAQIKD
                     VRLILDEMRLIKGAQELAVMRKAAQIATSAHSRAMRAVRPGKMEYEIEAELIHEFRRH
                     GAQAPAYTPIVASGANACVLHYVENRATLRDGDLLLIDAGCELDGYASDITRTFPVNG
                     KFKGPQKAVYEIVLAAQDAAIEAVKPGKSWIAPHQAALKVLAQGMIDLGLCKGSRDSV
                     IESGDYLRFYMHRTGHWLGLDVHDAGEYKRDGKWRKLKPGMVLTVEPGCYVRPGKNVP
                     ERFWNTGVRIEDDVLVTAKGREVLTHAAPKKVEDIEQLMRDHAA"
     gene            2301..3455
                     /locus_tag="VLL03_04355"
     CDS             2301..3455
                     /locus_tag="VLL03_04355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HSS46632.1"
                     /translation="MPPESAAIVIVGGGPVGAALALALRASSHSVLLLEARETTANDP
                     RPLALSHGSRLILERLKVWSAVKTPTPILNIHISQRGGFGSSRLTAQEADVPALGYVV
                     SYAELQSALQQALHGLEYLTGANVVRIEADENAVSAEFTHRGSTRKISAELLVLADGG
                     RGMEGVNGIKLREKDYHQCAVVTQVKTELPHRNMAFERFTPQGPVALLPFGEQFALVW
                     ATAPEDAGELLKFDDTAFLQRLHEHFGDRLGKFVAAAKRASFPLKLKYAADPTAQRVV
                     ILGNAAQTLHPVAGQGFNLGLRDAWELSEVILRTATQQLDTAEMLNAYRQRRKLDRGG
                     GIFFTDMLLRIFSNDLAPLRAARGMALTALDVLPAAKRFLMRRMIFGARG"
BASE COUNT          731 a          963 c         1067 g          715 t
ORIGIN      
        1 tttgcacggc tttatcaccg cgatggcaag gaaggctacc ttaaagacct accgctggtg
       61 atgcagtatt tgcgcaaagc ctgcgagcgc tattccgagc tcgcgcctct gctgaagttg
      121 ttggaggagc tcgaggggaa aacgcaaatc aagacgggct atacgtttta acttaccgca
      181 gagacgcaga gacacagagg cgcaaagttt tctatccttc caaacattta ttcatgctca
      241 gcgtctccgc gcctccgcgg tttaaaaaat catgagagcg atgatacttg ccgcgggtca
      301 cggcgaacgc atgcgcccgc tttccgacac cacgcccaag ctgttgctgc ctgtcggcga
      361 caagccgctc atcgcctgga ttctggaaaa tctcgcggcg gcggggttcc atgaaatcgt
      421 catcaatcat tcgcatttcg gcaagcaaat tgaatccgcg ttgggcgatg gcgcaggtat
      481 cggcgtgcgc atcgtttatt cgccggagcg gcaggcactg gaaaccgcag gaggcatagc
      541 gcaggccttg cctttgctgg gagcgcagcc gtttctggtg gtgaacggcg atatttactg
      601 cgacctcgat ttcaggcagc ttctgccgca gctcaaccgg atgagggaaa aacccgattc
      661 tgaccgcgct tatttggtgt tggtggataa ccccgggcat catcccgaag gcgatttcgc
      721 gctggaacaa aatcgggtga ccctaagcga aacgcaaaag cttactttca gcggcatcgg
      781 cttgtatcat cccgcgctgt ttgccggcgt gccacagggg ggcaaaatga aactcgcacc
      841 gctgttgctc gaccaaattg cggccggcaa agtcggcggc gagcattacc acggcttatg
      901 gcgggatgtc ggcacgcccg aacgtctgcg cgagctggat gaattgctaa agagccagcc
      961 tgcgcgcggc aagtaaggct agggtttata attctccacc atggacgatt actcgatttt
     1021 cgcgcagcgg cgcgccagac tcattaaaaa gatgcaggcg ggagtggcgc tcgtccccac
     1081 cgcggccgag cgcgtgcgca accgcgattc gcattatccc taccgcttcg acagctactt
     1141 ttattatttg agcggtttca ccgagcctgg cgccgtactg gtgctggtgg ccggcaagca
     1201 accgaaatct gttttatttt gccgcgacaa ggatccggaa cgcgaaatct gggacggccg
     1261 ccgttacggc ccgccagccg cgcgcaaacg tttccggttc gatgagggct gctcgctcaa
     1321 ccagctggat gaaatgatac cgcagctact cgccaatcag ccggtgctgt attacgcgat
     1381 gggcacggac gaagcatggg atgcgcgcat gctcggctgg cttaaccaag tgcgtgcgca
     1441 aacgcgggcg ggcgttactg cgccggcaca aatcaaggac gtgcgcctga ttctggatga
     1501 aatgcgcttg attaaaggag cgcaggagct tgccgtgatg cgcaaagcgg cgcagattgc
     1561 cacaagcgcg cacagccgcg ccatgcgcgc cgtccgcccg ggcaagatgg aatatgaaat
     1621 cgaggcggag ctgattcacg aatttcgccg ccacggcgcg caggccccgg cgtatacgcc
     1681 catcgtcgcc agcggcgcga acgcctgcgt gctgcattac gtggagaacc gcgccacctt
     1741 gcgcgacggt gatttgctgc tgatcgatgc cggctgcgaa ctcgacggct acgcctcgga
     1801 catcacccgc acctttccgg tgaacggcaa attcaagggc ccgcaaaaag cggtttatga
     1861 aatagttctg gcggcgcagg acgccgcgat tgaggctgtg aaacccggca agagctggat
     1921 cgcgccgcac caggcggcgc tgaaggtgct ggcgcaaggc atgattgatt tgggcttatg
     1981 caagggaagc cgggacagcg tgattgagtc gggcgattac ctgcgttttt acatgcaccg
     2041 caccgggcat tggctggggc tggatgtgca tgatgccggt gagtataaac gcgatggcaa
     2101 atggcgtaag ctcaagcccg gcatggtttt gacggtggaa cccggctgtt atgtgcggcc
     2161 ggggaagaat gtcccggaaa gattctggaa cactggagtg cgcatcgaag acgatgtgct
     2221 ggtcaccgcc aaagggcggg aggtgctgac tcatgccgcg cccaagaaag tcgaagacat
     2281 tgaacaatta atgcgcgacc atgccgcctg aatccgccgc tatcgtaatc gtaggcggcg
     2341 gcccggtcgg cgccgctttg gcgctggctt tgcgcgccag cagccacagt gttctgctgc
     2401 tcgaagcacg cgaaaccacc gcaaatgacc cgcggccgct ggcgctctcc cacggcagcc
     2461 gcctgattct tgagcggcta aaagtctgga gcgcggtcaa aactccgacg cctatcttga
     2521 acatccatat atcccagcgc ggcggcttcg gctcaagccg actcaccgcc caggaagccg
     2581 atgttccggc attgggttat gtggtttcct atgcagagtt gcaatcggcg ttgcaacagg
     2641 cgctgcatgg gcttgagtat cttaccggcg caaatgtggt gcgtattgag gcggatgaaa
     2701 atgcggtgag cgccgaattc acccatcgcg gcagtacccg caaaatcagc gcggaactgc
     2761 tggtgttggc cgacggcggg cgcggtatgg agggagtcaa cggcatcaag ctgcgggaaa
     2821 aagattacca ccagtgcgcc gtggtgactc aggtcaaaac cgaattgccc catcgcaaca
     2881 tggcgtttga acgcttcacg ccgcaagggc cggttgcgtt gctgccgttt ggcgagcagt
     2941 tcgctttggt ctgggctacc gctcccgagg atgcgggaga gttgctcaag tttgatgaca
     3001 cagcgttttt acagcgcctg catgaacatt ttggcgatcg gcttgggaaa tttgttgcgg
     3061 ctgcaaaacg cgcaagtttt ccgctcaagc tcaagtatgc cgctgatccg acggcacagc
     3121 gcgtggtaat ccttggcaac gcggcgcaaa ccctgcatcc cgttgcaggt caaggcttta
     3181 acctgggatt gcgcgatgcc tgggagttga gcgaagtgat actgcgtact gcaacacagc
     3241 aactcgacac agcggagatg ctgaatgctt accgccagcg cagaaagctg gatcgtggcg
     3301 gcggaatttt ctttaccgac atgttgttgc ggattttcag caacgatctt gcgcctttgc
     3361 gcgcggcgcg tggtatggcg ttgactgcac tggatgtttt gccagcggcg aaacgtttct
     3421 tgatgcgcag gatgattttc ggcgcgcgtg gctgattaaa tttttttgcc ttgcac
//
LOCUS       DATIOW010000134        18932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_164994, whole genome shotgun sequence.
ACCESSION   DATIOW010000134 DATIOW010000000
VERSION     DATIOW010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 18932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18932
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164994"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="VLL03_04360"
     CDS             complement(<1..1086)
                     /locus_tag="VLL03_04360"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSS46633.1"
                     /translation="MRKQVIMWSLFILLLHAAFLGGYWLNQWLNPKSAPAPQLTQPTA
                     AQTEGSPAQPTASQTEAQPTASQQDKKILYWHDPMFPQQRFNKPGKSPFMDMQLVPVY
                     ADEANDQGGVSINPRLTQNLGVRTVPVEIGVFARQVHTVGTVMPDEHRIEVVQSRASG
                     WVERLYVKTVNEKVARGQLLAEIYAPELLAAQEEFVLALKHAGGAGDPLVQAARARLS
                     FLGLSVEQIAALEKSEKPLRRVAFYSPINGIVAQLGVRQGMAVTPGMVMFNLVDLSSV
                     WVTAEVVENQAGWIAEGNRAEIRIPAYPGKVFAGRLDYIYPELMAATRTVKARIVLKN
                     PEGLLKPDMFVDVMIDAQSRENTLMVPT"
     gene            complement(1095..2342)
                     /locus_tag="VLL03_04365"
     CDS             complement(1095..2342)
                     /locus_tag="VLL03_04365"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSS46634.1"
                     /translation="MSFKIRARLALLVLLNLLIPAENAAAATPLMLDEAVNLALANQP
                     LIAGQQAAIHAAEKNAVAAGQLPDPKLKFGLSDLPVTGSEAYSVRRDNFTEFKVGVSQ
                     DFPRAEKRRLRGAMAQLEAQRDTEELEVIRRAIKRDAALAWLEVFYPERAISLLQALE
                     REGQVQLDALNIAYRAGRASQADVRASQVALDLLKDREADFARQASRSRASLARWIGA
                     EASRPLAEILPDLPMPLDRGALLAHVENHPHLSFIDKQVVMAQTDVELARQAYKPDWS
                     IEVSYGNRPAFPDFFSVQFGIDLPVLTKNRQDPALASKLSLLDKSRSLKEDALRALKA
                     DAERDYADWQSSLERVQRFDANILPQAKSRVDAALAAYQSGHGDLNAVLEARRAEFDL
                     QLQRLGLQVDAARAQLQLQYFSQ"
     gene            complement(2409..2774)
                     /locus_tag="VLL03_04370"
     CDS             complement(2409..2774)
                     /locus_tag="VLL03_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46635.1"
                     /translation="MRRFLSIVSRFTLGLMLFAKLSLVVHACMLAGNQPASALDPHAN
                     APCHQAGSFNPNVCLSHCTESNQSTAAHMLPALPAATLAVLSVTPVKTAAALQTQTAA
                     QSLNITDPPRTIRFCCFLN"
     gene            2974..4302
                     /gene="argA"
                     /locus_tag="VLL03_04375"
     CDS             2974..4302
                     /gene="argA"
                     /locus_tag="VLL03_04375"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HSS46636.1"
                     /translation="MLHSTPTDAIAFVQWLRSVAPYIHAFRGRTFVIAFGGEVVGGGG
                     FVELTHDFNLLHSLGVRLVLAHGARNQIEARLKAFRHASRYVRGLRVTDDTALQCAKE
                     AVGRIRVEIEALLSMGLPNSPMAGAQIRVASGNFVTAKPIGVIGGVDLQHTGEVRKVD
                     AQAIQQRLGDGEMVLLSPLGYSPTGEIFNLMLEDVATSAAIALHADKLIFLMDEAGVT
                     DTSGVLLNELTARDAEKLLAKSGKLAGDVQLFLPCAVRACSQGVPRAHLLSRHVSGAV
                     LLELFTHGGIGSMVTQSGLEKLRQAHIEDAGGILALIEPLEKEGVLVRRSRARLEREI
                     SRFSVIEHDSVIIGCAALYPFPGDGAGELACLVVSEDYRNGGYGGTLLKHVEKQAREA
                     GMKRLFVLTTRAAHWFMERGFRETGLEQLPKEKQALYNYQRRSKVFLKRL"
     gene            4346..7135
                     /locus_tag="VLL03_04380"
     CDS             4346..7135
                     /locus_tag="VLL03_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSS46637.1"
                     /translation="MIGFFRTTSLRLKLVLISFVVEAVMLTLLVANSVWLIDNVLDEQ
                     ARLQMKMLGPLLNVSLAGPLADGDIASARQILAEIRAKEKLAYAELLNDKGDILAGAE
                     PSAASRYESWVTIEQEGKSYGRLRLGISTDFLAAAKRKLFLESSVIAVAEVLLSLLLF
                     LGLGFWLTRNLKELTDAGKAVAVGNYAVRVQVVSSDEVGRLSTTFNAMAEAVQNQVKS
                     LKEGEAKFRAIANYSYDWELWADPTGKVVWVNPSVQRLTGYSPQECLEMQGFPAAIIH
                     PEDSDRFREGSRRILDNKTTSGSDFEFRVLRKDGTGFWAAADWQPIYGAHGEHLGVRA
                     SIHDITERKQAELKILATVQQLEQAQQDQQRYYAQAQEERARFNSLLNAMDLGILFVG
                     NQNSTAYYNPAFLRVWELPADAQLAGTPTDELLATLEKQLARSADYAQHVHEIIQTKE
                     NFHNYELTLVDGRMLLETSYPVPGADGRYLGRMWLFKDVTQERRTADQIIYLAERDAL
                     TGLYNRHRFQEELTRMLADAGRRNSTMGLLFFDLDEFKVINDTYGHRAGDSVLIRVAA
                     EVGTQVRRNEIFSRLGGDEFAILVPDVREDELQVLAERVVHSIPQIPFHFEGHNLRLS
                     TSLGIAVFPDHAQNAEELVAHADAAMYQAKEAGKNAWRVYALHSDTSRQMLTRITWNE
                     RIRSALRNNLLYLDFQGVYRADDGLLSHIEALVRMKDEENLGNIIMPVSFIPLAEKSG
                     AILEIDRWVIGQCIGLLATSGKVPPISVNISARSFDDPKLPHYISDELRRLDVNPKRL
                     IVELTETSAVSDLHDAQRFIEALHQAGCRVCLDDFGSGFSSFAYLKHLQVDIVKIDGL
                     FIRNLPNDSDAQVFVRAMVDVARGMRKITVAESVEDKETLEMLKGFGVDMMQGYYLEK
                     PHADHVAVTHIGTAV"
     gene            7164..7442
                     /locus_tag="VLL03_04385"
     CDS             7164..7442
                     /locus_tag="VLL03_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HSS46638.1"
                     /translation="MVRMVKCVKLGHEAEGLDVPPYPGELGKRIYGNVSKQAWGGWLE
                     HQKMLINENRLNLADPKARKYLAEQMEKHFFGAGAEVASGYVPPKPGK"
     gene            complement(7478..8134)
                     /gene="rpiA"
                     /locus_tag="VLL03_04390"
     CDS             complement(7478..8134)
                     /gene="rpiA"
                     /locus_tag="VLL03_04390"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HSS46639.1"
                     /translation="MTQDELKQTVARAAIQYVPEGCVVGVGTGSTANYFIAELGKIRH
                     KLEGAVASSEATAQRLKNLGIPVLDLNSVNELPVYVDGADEITKHLHMIKGGGGALTR
                     EKIITAVAKKFVCIADRSKLVDVLGKFPLPVEVIPLARGYVARQLVKLGGSPQLRLGF
                     TTDNGNIIIDVYGLTLLNPPELEEKLNQIDGLVCNGIFAKRPANVLLLGTADGVKTYT
                     "
     gene            8273..9784
                     /gene="ilvA"
                     /locus_tag="VLL03_04395"
     CDS             8273..9784
                     /gene="ilvA"
                     /locus_tag="VLL03_04395"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HSS46640.1"
                     /translation="MHTDYLEKILASRVYDVAHETPLELAPGLSARMHNRLLLKREDM
                     QPVFSFKLRGAYNKMVQLSEEELQRGVIAASAGNHAQGVALAAQKLGCKAVIVMPVTT
                     PQIKVQAVATRGAEVVLHGDSYDEAHAAARKLEKARKLTFIHPYDDPDVIAGQGTVGM
                     EILRQCSKPIHAIFVAVGGGGLISGVAAYVKRLQPEIKIIGVEPQDADAMTRSLKAGR
                     RVKLSQVGLFADGVAVKEVGKETFRLCKKYVDDMILVDTDAICAAIKDVFEDTRSILE
                     PAGALSVAGAKAYVQRKKLKGKTLVAIACGANMNFDRLRFVAENAELGERREAILAVT
                     IPERPGSFKKFCSLLGKRNITEFNYRIADPDEAHIFVGIQVHNSEETAKLVTTLKRHH
                     LRTLDFSDNEMAKLHVRHLVGGHALLADNELLYRFEFPERPGALMKFLNSMRSDWNIS
                     LFHYRNHGADYGRVLVGMQVPPREMGEFRQFLAKLGYAYWDETKNPAYQLFLS"
     gene            complement(9791..10483)
                     /locus_tag="VLL03_04400"
     CDS             complement(9791..10483)
                     /locus_tag="VLL03_04400"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HSS46641.1"
                     /translation="MNAAKLKTWRKELRADLIARRLAANPAERGTWNDAISNHLERAF
                     PNLARGVVVFCWPHKNEFDARFLMRKLRERGAVTALPVVVAPKTPLIFREWHPGVKMA
                     NGVLDIPYPAQSSELVPDTVLLPMNGFDEEGYRLGYGGGYFDRTLASLNKKPLVIGVT
                     YELARLATIHPQAYDLPMDFIVTERGVYERGSGKIELLARHGQEYEPGPYSSPACYAH
                     EIAPDYFDGEKG"
     gene            10591..12468
                     /locus_tag="VLL03_04405"
     CDS             10591..12468
                     /locus_tag="VLL03_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HSS46642.1"
                     /translation="MPARADQGDDFLKARAAYAAGNISRLDEYAKRLQGYVLEPYIAY
                     WQLRSRLEDAPPETVRAFIWRYSDTLLADRMRAEWLKVLARKGRWELFAAEFPPVRNE
                     STELTCYALQGRLRNKDDTALIEARSLWFSAEEQPDSCAPLFQEMFIRNVLGIEDVWQ
                     RLRLALEAGNLGAARRIIQYLPAKLAPNPRLFKSIANRPERYLAKPTYNLKTRLGHEL
                     TLFALYRLARNEPKRALSFWNKLTNAFDEEEQQYVRGQLAYQAALSHEPDALNWFANV
                     PVNLLTDQQLGWKTRAALRTLDWQAVVDGIEAMTVIEARDPTWRYWKARALRELGKNA
                     ESNAILQELANDHYYYGQLASEELGVTHSAPAVAYKPSEQELSAIEQLPSIQRALALY
                     QLELRYEGNPEWQWAIRSFDDLQLVAAAEIARRHQLYERAVFTANQTLQVHDFGLRYP
                     APYREHVQEYVRQSELDEAWLYGVIRQESRFERDAHSSAGARGLMQLMPATAKWVARR
                     IGKGYRHTKANDLETNLSLGIFYLKHILDISGGQMVLASAAYNAGPTRARQWGADVPL
                     EGAIYVETIPINETRGYVKKVLSNTLYYASILGKQPLTMKSLLGTIPPRAQSRSLQDE
                     R"
     gene            12474..13421
                     /locus_tag="VLL03_04410"
     CDS             12474..13421
                     /locus_tag="VLL03_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HSS46643.1"
                     /translation="MKLDKVCVVGGSGFVGRHIVHLLAAQEKKVRVTTRNRERSKELI
                     VLPTVDVVQADVHDDAQLEQMLEGMDAVINLVGILHEERSGDFQRVHVELPQRIARTC
                     ERLGIPRLVHMSALNADVNGPSVYLRSKGEAEAALKNRAKAPAVTIFRPSVIFGREDK
                     FLNLFAKLLRFFPVLFLACPNARFQPIFVEDVARAFVDSLARLETFGQSYDLCGPKAY
                     TLRELVKYMAVLTGRHRLIVGLSDRLSYLQAWSMEFLPVKLMTRDNYYSMRIDSVCDC
                     AFPEIFGFQPAALEAAVPLYFAGGTPRGRYRWFRYKAGR"
     gene            13452..14675
                     /locus_tag="VLL03_04415"
     CDS             13452..14675
                     /locus_tag="VLL03_04415"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HSS46644.1"
                     /translation="MKIYTVGGAVRDELLGLKVTDRDYVVVGSTPQEMQRLGFKPVGK
                     DFPVFLHPKTHQEYALARTERKTARGYRGFRIYSAPDVTLEQDLARRDLTLNAIARDE
                     HGKIIDPYGGVKDLQAGVLRHVSPAFAEDPVRILRVARFAARYGFKVAGETMALMRQM
                     VKNGEVDALVAERVWQELSKGLMEKKPSRMFEVLRECGALAKILPEVDRLFGVPQAKE
                     QHPEVDTGRHIMLVIDYAAAKNFSLPVRFAALTHDLGKGVTPQKDWPRHPGHETKSVE
                     LVKALCKRIRVPAACRDLAVITARHHGEAHRALELDAASLVKLLQATDALRKPQRFAE
                     FLDACGCDFHGRPGYQTRVFAQAAHLQSALRVAKGVDAGKIAKRLKDSRQIAARIRIA
                     RTAAVETWLAGERIV"
     gene            14734..15207
                     /locus_tag="VLL03_04420"
     CDS             14734..15207
                     /locus_tag="VLL03_04420"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HSS46645.1"
                     /translation="MLKPVLIASLLLVPAFALADDAGKPGEKKHRMEEYFKKADKDND
                     GTLTRDEAKAMPHVAKHFDEIDADKDGTVSMDEIRAFAKAQHKDAHHRGEERFKKADK
                     DNDGTLTKDEAKAMPNVYKNFDAIDTDKDGTVTLDEIHNYMKSRRETRQGQPKAY"
     gene            15336..15944
                     /locus_tag="VLL03_04425"
                     /pseudo
     CDS             15336..15944
                     /locus_tag="VLL03_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
     gene            16010..17284
                     /locus_tag="VLL03_04430"
     CDS             16010..17284
                     /locus_tag="VLL03_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HSS46646.1"
                     /translation="MMANPAMGLLVPIQIGKYPVIRELGRGMTGRVYLARDPFGGREV
                     AIKLVSTEDIGNPEDRKVFRKAFLNEASLAGKLSHPHIVGILDAVVEEAMSYIVMEYI
                     DGSTLDQYCSVDNLLPLEKVVEITFKSSRALEFANHAGVIHRDIKPANILMQSNGDIK
                     ISDFGAALLHDAEETQLTGVGSPAYMSPEQLREQPLTLQTDIYSLGVVMYRLLTGKFP
                     FNAASKASLIYQILNIDVLPPSVHRSNLPAQLDSIVLCALQKDLSLRYRNWNEFGRDL
                     TGAFKNLVMPGENIPETEKFDTIKSLPFFRDFHDVQIWEALRITAWKKLPKGTVIIRE
                     GDQGDCFYVLAEGEVTVSKQGKPLNALKRGDCFGEMLYFRATTAQRKTSVIASTPVTV
                     VEIKASSLTQASNPCQNQFNKAFLSILIERHG"
     gene            complement(17400..18059)
                     /locus_tag="VLL03_04435"
     CDS             complement(17400..18059)
                     /locus_tag="VLL03_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46647.1"
                     /translation="MRLKLYCLVLFFAVSGAALAQQQESVKTAGPAKWEQGPHGLMLL
                     RILPPGPKPEELPQPASAGARTLTRYCVQCHYLPSPYMHTPERWEVVVERMVRRMQGE
                     GNLGVVMKELMEDMETPSEAEVENLLAYLSKYGQKAIDPGRYPDLKTTAAGRTYTEAC
                     IQCHELPDPKRHTATEWPGVVERMQKNMAWVGVIKGKGLNPHPLKVKDILEFLKRHSR
                     P"
     gene            18150..>18932
                     /locus_tag="VLL03_04440"
     CDS             18150..>18932
                     /locus_tag="VLL03_04440"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSS46648.1"
                     /translation="MSSIARYHALIIGTGQSGKPLAGTLARAGWKTAVIEREHVGGTC
                     VNVGCTPTKTMVASARVAYLARCAANYGVNTGDVSVDMAKVYARKKAIVESFRKGGQD
                     RLESTPNLELIFGVARFKDHRTVEVRLNGGGTREMTADRIVINTGGTPALPPLPGLSE
                     ARPLNSSTIMELQALPEHLLVLGGSYIGLEFAQMFRRFGSRVTIIHRDDRLINREDPD
                     VSEEVQEILRQDGLEILLDAQVVRISRQPNGRTEVNIKTPAGE"
BASE COUNT         4000 a         5366 c         5696 g         3870 t
ORIGIN      
        1 ggtaggcacc atgagcgtat tctcccggct ctgtgcgtcg atcatgacat ccacgaacat
       61 atcgggcttg agcagccctt ccggattttt cagcacgatg cgcgctttca ccgttcgtgt
      121 cgctgccatc agttccgggt agatataatc caggcggcct gcgaacactt ttcctggata
      181 agcgggaatg cggatttcag cgcggttgcc ctcggcaatc catcccgcct gattttccac
      241 cacctccgcc gtgacccaga ctgaggaaag atccaccaaa ttgaacatca ccatgccggg
      301 agtgaccgcc atgccctggc gtaccccgag ttgtgcgacg atgccgttga tcggtgaata
      361 aaacgccact cgccgcaagg gtttttcgct tttttcaagc gctgcgatct gttctacgga
      421 aagtccgagg aaagaaagcc tggcacgcgc ggcctgcacg agaggatcgc cggcgccgcc
      481 tgcgtgcttg agcgcaagta caaattcctc ctgtgccgcc agcagttcgg gggcgtaaat
      541 ttcagccagc aattgaccac gcgctacctt ctcgttcact gtcttgacat acaggcgctc
      601 cacccagcct gaggcgcggc tttgcacgac ctcgatgcga tgctcgtcgg gcatcaccgt
      661 gccaacggtg tgcacttgtc ttgcaaacac gccgatttcc actggtacag tgcgaacgcc
      721 gaggttttgc gtaaggcgcg gattgatgct gaccccgcct tgatcgttcg cttcatcggc
      781 gtacaccggc acgagctgca tgtccatgaa aggcgatttg cccggcttgt tgaagcgctg
      841 ctgcgggaac atcgggtcgt gccagtagag gatttttttg tcctgctgcg atgcggttgg
      901 ctgcgcttcg gtttgcgatg ccgtcggttg cgccggtgat ccttcggttt gcgctgccgt
      961 tggctgcgtc aattgtggtg cgggcgcgga ttttggattc agccattgat tcagccaata
     1021 gccgcccaag aacgcggcgt gcagcaatag gataaaaaga ctccacataa ttacctgttt
     1081 gcgcatgacg gctcctattg cgaaaaatat tgcaactgca attgcgcccg cgcggcgtcc
     1141 acttgtaacc ctaagcgctg caactgcaaa tcgaattcgg cgcggcgtgc ttccaacacc
     1201 gcgttcagat cgccgtggcc ggattgatat gcggcgagcg ccgcgtccac ccggctcttc
     1261 gcttgcggca ggatgttcgc gtcgaagcgc tgcacgcgtt ctaggctgga ttgccagtcg
     1321 gcgtaatcgc gctcggcgtc ggcttttaac gcgcgcaatg cgtcttcctt cagcgagcgc
     1381 gatttgtcaa gaagtgaaag tttcgatgcg agcgccgggt cctggcggtt tttggtcaac
     1441 accggcaaat caatgccgaa ttgcacagag aaaaaatcgg gaaacgccgg gcggttgcca
     1501 taactcactt ctatgctcca gtcaggtttg tacgcctggc gcgcaagttc cacgtcggtt
     1561 tgtgccatca ctacctgctt gtctatgaaa cttaaatgcg gatgattttc cacatgggcg
     1621 agcagtgccc cccggtccaa cggcatgggt aaatcaggca ggatttctgc aagcggacgc
     1681 gaagcttcag caccaatcca acgcgcaaga gaagcgcgtg agcgcgaggc ttgccttgca
     1741 aaatcggcct cgcggtcctt gagcaaatcc agcgctactt gcgaggcccg cacatcagct
     1801 tggcttgctc ggcccgcgcg gtaggcgatg ttgagcgcat caagctggac ctgaccttca
     1861 cgctcgagcg cctggagcag gctgattgcg cgttcgggat aaaacacctc cagccaggcc
     1921 agcgcggcat cgcgcttgat ggcgcggcgg atgacttcaa gttcttccgt gtccctttgc
     1981 gcttccagtt gtgccatcgc gccacgtagt ctgcgttttt ccgcgcgcgg gaaatcctgg
     2041 ctgacgccaa ccttgaattc ggtgaagtta tcgcgccgca cgctgtacgc ttccgagccg
     2101 gtgaccggca ggtcgctgag cccgaatttc agcttggggt cgggtagttg tcctgccgcc
     2161 accgcgtttt tttcggcggc atgaatcgcc gcctgctggc cggcaatgag cggttggttg
     2221 gcgagcgcaa gattcaccgc ttcgtccagc ataagtggcg tggcggccgc ggcgttctcg
     2281 gccggaatca gcaggtttaa caatacaagg agcgcaagcc gcgcccggat tttgaacgac
     2341 atgatgcctc cgcaaacgaa atatccactc gccacatatg cggcgagtgc gaaacgttta
     2401 cgcggaagct aattcaggaa acagcagaaa cggatggttc gcggagggtc ggtgatgttg
     2461 agagattgtg ccgcagtttg ggtttgtagc gcggcggcgg ttttaaccgg ggttacgctc
     2521 aacacggcca gggttgcggc aggtaaagcc ggcagcatat gagcggcggt gctttgattg
     2581 gattcggtgc aatggctaag gcagacgttg ggattgaacg agcctgcctg atggcaaggc
     2641 gcatttgcat gcggatcaag cgcggaagcg ggctggttgc ccgcgagcat gcaggcgtgc
     2701 actaccaggc tgagcttggc aaacagcatc agccccaggg taaagcggga gacgattgat
     2761 aaaaaacgcc gcatgctcgt gccgcctcgc aatgatgagg tttgactgtg accttactgt
     2821 aactgttagg ttccgcacag tactctagcc gctctggttc cggctagcaa ccggaaaatg
     2881 cagtttatcg cttttcccag aggtgtttat ccattatgat tcgattataa aagcgaacaa
     2941 actcggctgc tttgcgcggc ctttcctgcg actatgctgc actcgacccc cactgacgcc
     3001 attgccttcg tgcaatggct gcgttcggtc gcgccctata ttcacgcgtt ccgcgggcgc
     3061 accttcgtca tcgcctttgg cggcgaggtg gtgggcggcg gcggctttgt cgaacttacc
     3121 catgatttca atctgctgca cagcctgggc gtgcgcctgg tgctggcgca cggtgcgcgc
     3181 aatcaaatcg aagcgcggct caaagcgttc aggcatgcaa gccgttatgt gcggggcctg
     3241 cgggtgaccg acgataccgc tttgcaatgc gccaaggaag cggtggggcg cattcgcgtg
     3301 gaaatcgagg cattgctttc catgggtctg cccaattcgc cgatggccgg cgcgcaaatc
     3361 cgcgtggcga gcggcaattt cgtcaccgcc aagccgatag gcgtgattgg cggcgtggat
     3421 ttgcagcaca ccggcgaagt gcgcaaagtg gacgcgcagg cgattcaaca gcgtcttggc
     3481 gacggcgaga tggtgctgct ctcgccgctt ggctattcgc cgaccgggga gattttcaac
     3541 ctcatgctgg aagatgtggc tacgtcggcg gcgatcgcgc tgcatgccga caagctgatt
     3601 tttctcatgg acgaagccgg ggtcaccgat acttcgggcg tgctgctgaa cgagcttact
     3661 gcgcgcgatg cggaaaagct gctggcaaaa agcggaaagc ttgcgggtga tgttcaactt
     3721 tttctccctt gtgcggtgcg cgcctgcagc cagggtgtgc cacgagccca tcttttgtcg
     3781 cgtcatgtga gcggggcggt gctgctggag ctttttaccc atggcggcat cggcagcatg
     3841 gtgacgcaat ccggattgga aaaactgcgt caggcacaca tcgaagacgc gggcggtata
     3901 ctggctctga ttgagccgct ggagaaagaa ggcgtgctgg tgcggcgctc ccgcgcgcgg
     3961 ctggaaaggg aaatcagccg cttcagcgtg atcgagcacg atagcgtgat tatcggctgc
     4021 gccgcgcttt atccttttcc cggggacggg gcgggtgaat tggcgtgtct ggtggtaagc
     4081 gaggattacc gcaacggcgg ctacggtggt acgctgctta aacacgtgga aaagcaggcg
     4141 cgcgaagcgg gaatgaagcg gcttttcgta ctcaccacac gcgccgcgca ctggtttatg
     4201 gagcgcggct tcagggagac gggtctcgaa cagctgccga aagagaagca ggcgctctac
     4261 aattaccagc gccgctcgaa agtgtttttg aagcgcctgt gacaaaagtg ttgtcacgcc
     4321 tatggcgcac agggggagtt aaaccgtgat agggtttttc cgcaccacat cgctgcgcct
     4381 gaagctggta ttgatcagct tcgtggtgga agcggtgatg ctcaccctgc tggtcgccaa
     4441 cagcgtctgg cttatcgaca atgtgcttga cgagcaagca cgcttgcaga tgaagatgct
     4501 gggccccctt ctcaacgttt cgctcgccgg gccgctggcc gacggtgaca tcgcttccgc
     4561 cagacaaatc ctcgcggaga tccgggcgaa agaaaaactc gcctacgcgg aactgttaaa
     4621 tgataaaggt gatattctcg ccggcgcgga accgtcggca gcctcgcgtt acgaaagctg
     4681 ggtgacgatc gagcaggaag gcaaaagcta cggccggctg cgactcggca tttccaccga
     4741 ttttctcgcc gccgccaagc ggaaactttt tctcgaaagt tcggtgattg ccgttgccga
     4801 ggtgctgctt tcactgctgc ttttcctggg gctcggattt tggctgacgc ggaaccttaa
     4861 ggaactgacc gatgcgggaa aagcggtggc ggtcggtaat tatgcggtgc gcgtacaggt
     4921 ggtgtcgtcc gacgaagtcg gccggctttc caccaccttc aacgccatgg ccgaggcggt
     4981 gcagaatcag gtcaaatcgc ttaaagaggg tgaagcgaaa ttccgcgcga ttgccaatta
     5041 cagctatgac tgggagctgt gggccgaccc caccggcaag gtagtgtggg tgaacccttc
     5101 ggtgcagcgg ctcaccggct attccccgca ggaatgtctg gagatgcaag gcttccccgc
     5161 ggcgatcatc catcccgagg acagcgatcg cttccgcgaa ggatcgcgcc gcattctgga
     5221 caacaaaacg accagcggca gcgacttcga gttccgcgtg ctgcgcaagg atggcactgg
     5281 cttctgggcg gcagcggact ggcagccgat ttacggcgcc cacggcgaac atctcggcgt
     5341 ccgcgccagc attcacgaca tcactgaacg caagcaggcg gaactgaaga tcctggccac
     5401 ggtgcaacaa ctcgaacagg cacagcagga tcagcagcgg tactacgccc aagcgcagga
     5461 agaacgagcg cgcttcaatt cattacttaa cgcgatggat ttgggcatcc tgtttgtcgg
     5521 caaccagaac agcacggctt attacaatcc ggcgttcttg cgcgtttggg agctgcctgc
     5581 ggatgcccag cttgccggca cgccgaccga cgagctcctg gctactttgg aaaaacagct
     5641 ggcgcgttcc gcggactatg cccagcatgt gcacgaaata attcagacca aggagaattt
     5701 ccacaactat gaactcacgc tggtcgatgg gcgcatgttg ttggagacga gctatcccgt
     5761 gccgggcgct gatggccggt atctgggccg gatgtggcta ttcaaggatg tcacccagga
     5821 gcgccgcacc gccgatcaga taatttatct ggccgagcgc gacgcgctga ccgggcttta
     5881 caaccggcac cgcttccagg aggaattgac gcgtatgctg gcggacgccg ggcgccgcaa
     5941 ttccacgatg gggcttttgt tttttgacct cgacgaattc aaggtcatta acgacaccta
     6001 cggccaccgc gcgggcgact ccgtactgat acgtgtggcc gcggaagtgg gcacgcaggt
     6061 gcggcgcaac gaaatcttca gccgcttggg gggagacgag ttcgccattc tggtcccgga
     6121 tgtgcgcgag gacgaattgc aggtgctggc ggaacgcgtg gtgcattcga ttccgcagat
     6181 cccgttccat ttcgaggggc ataacttgag gctgtccacc agcctgggga ttgcggtgtt
     6241 tcccgaccat gcgcaaaacg cggaggagct cgtagcgcac gccgatgcgg cgatgtacca
     6301 ggccaaggaa gcgggcaaaa acgcctggcg cgtctacgcc ctgcatagcg acacctcgcg
     6361 acagatgctc acccgcatca cctggaacga acgcatccgc agcgcgctgc gcaataacct
     6421 gctctatctg gatttccagg gggtgtaccg cgccgatgat ggcctccttt cgcatatcga
     6481 ggcgctggtg cgaatgaagg atgaggagaa tctcggtaac ataatcatgc cggtgagctt
     6541 tatcccgctc gcggaaaaaa gcggagcaat cctggaaatc gaccgctggg taattggcca
     6601 atgcatcggc ctgctggcga cctcgggcaa agttccgccg atctccgtga acatttccgc
     6661 ccgctcgttc gacgacccga aactgccgca ctatatcagc gacgaactgc gccgcctcga
     6721 cgtgaatccc aagcggctga tcgtggagct caccgagact tcggcggtgt cggatttgca
     6781 cgatgcgcag cgcttcatcg aagcgctgca ccaggctggt tgccgcgtct gcctggacga
     6841 cttcggcagc ggcttttcct cgtttgccta tctcaagcac ctgcaagtgg atatcgtgaa
     6901 aattgatggt ttgtttatcc gcaacttgcc caacgacagc gacgcccagg tattcgtccg
     6961 ggccatggtg gatgtggcgc gaggcatgcg caagattacc gtggcggaat cggtggaaga
     7021 caaagaaacg ctggagatgc tgaaaggttt cggcgtggat atgatgcagg gctattacct
     7081 ggaaaagccg cacgccgatc atgtggcggt aacccacata ggaacggcgg tataatgaaa
     7141 cgatagtttc cggaggaatg ctgatggtca ggatggtgaa atgcgtaaag cttgggcacg
     7201 aagcggaagg cctggatgtg ccgccctatc cgggagaact gggaaaacgc atttatggga
     7261 acgtctcgaa acaggcgtgg ggaggttggc tggagcacca gaagatgctg attaacgaga
     7321 accgcctcaa tctcgccgat ccgaaagcaa gaaaatatct cgccgagcag atggaaaagc
     7381 atttcttcgg cgcgggcgcg gaagtggcgt ccggctacgt gccaccgaaa ccaggcaaat
     7441 gaagaagtga aaaggtgaag aggtgaagac ttcgcgtcta ggtgtaggtc ttcaccccgt
     7501 ccgcagttcc caaaagcagc acgttcgccg ggcgcttggc gaaaatcccg ttgcatacca
     7561 agccgtcgat ttgatttagt ttttcttcaa gctccggggg attgagaagc gtgaggccgt
     7621 acacgtcgat aatgatgttg ccgttgtcgg tggtgaagcc taggcgcagc tggggggagc
     7681 cgcccaattt aacgagctga cgcgccacat agcctcgtgc cagcggtatc acttccaccg
     7741 gcagcgggaa tttgcccagc acatcgacca gcttgcttcg atcggcaatg cacacgaatt
     7801 tcttcgccac cgcggtgatg attttctccc gagtcaatgc tccgcccccg ccttttatca
     7861 tgtgcaggtg cttggtgatt tcatccgcgc catccacata aaccggcagc tcgttcacgc
     7921 tgtttaaatc cagcactgga atgcccaggt ttttcaaccg ctgcgccgtg gcttcggagc
     7981 tggctaccgc gccttccagc ttgtgcctga ttttgccgag ctcggcaatg aaatagttgg
     8041 cggttgagcc ggtgcccacg ccgactacgc agccttccgg cacatactgg attgccgcgc
     8101 gcgccaccgt ctgtttcaat tcatcctggg tcattttagt tctctaggca caggcggcta
     8161 gaatagcgtc tggctgaatt ttgcacaaat cagcggcaaa cagctaaacg ctgatagctg
     8221 acagctgaaa atcctgctaa cctttgcgct ggtttgtgtc atctattttg atatgcacac
     8281 ggattatctg gaaaaaattc tcgcttcccg cgtctacgat gtggcgcacg agacgccact
     8341 ggagcttgcg ccaggccttt ccgcgcgaat gcataatcgt ttgttattga agcgcgaaga
     8401 catgcagccg gtgttttcct tcaagctgcg cggcgcctac aacaagatgg tgcagctttc
     8461 cgaggaggag ttgcaacgcg gagtaatcgc cgcttccgcc ggcaaccacg cgcaaggcgt
     8521 ggcgctggca gcgcaaaagc tcggctgcaa ggcggtcatc gtcatgccgg tgaccacgcc
     8581 gcaaatcaag gtgcaagcgg tggcgacgcg cggcgcggaa gtcgtgttgc atggcgactc
     8641 gtacgacgag gcgcatgctg ctgcacgcaa gctcgaaaaa gcgcgcaagc tcactttcat
     8701 tcacccctac gatgacccgg atgtgatcgc cggtcagggc acggtgggca tggaaatcct
     8761 gcgccagtgc agcaaaccca ttcacgcgat tttcgttgcc gtaggcggcg gcgggctgat
     8821 ttccggagtg gcggcgtacg taaaacgttt gcagcctgaa ataaaaatca tcggtgtgga
     8881 gccgcaggac gcggacgcca tgacgcgctc tctgaaggcc ggccggcgcg taaaactcag
     8941 ccaggtggga ttatttgccg acggcgtggc ggtgaaggaa gtaggcaagg aaaccttccg
     9001 cctgtgcaaa aaatacgtgg acgacatgat tctggtggac accgatgcga tttgcgccgc
     9061 catcaaggac gtgttcgagg atacccgctc gatactcgag ccggcgggcg cgctctcggt
     9121 ggccggtgcc aaggcctatg ttcagcgcaa gaagctcaag ggcaaaacgc tggtggcaat
     9181 tgcctgcggc gccaacatga atttcgaccg tttgcgcttc gttgccgaga acgccgaact
     9241 cggcgagcgg cgcgaagcga tccttgccgt gactattccc gagcggccgg ggagcttcaa
     9301 gaaattctgc tcgctcctgg gcaagcgcaa catcaccgaa ttcaactatc gcattgccga
     9361 cccggatgaa gcgcatatct tcgtcggcat acaggtgcat aacagcgagg agaccgcaaa
     9421 actggtcacc acactgaagc ggcatcacct gcgcacgctg gatttttccg acaacgaaat
     9481 ggccaagctc cacgtacgcc atctcgttgg cggccatgca ttactcgccg acaacgagtt
     9541 gctttaccgc ttcgaatttc cggagcgccc cggagcactg atgaagtttt tgaacagcat
     9601 gcgctcggac tggaacatca gcttgttcca ctaccgcaac catggcgcgg actacggccg
     9661 ggtgctggtg ggcatgcagg tgccgccgcg ggagatgggg gaattccgcc aatttctcgc
     9721 caagctcggt tacgcctatt gggacgaaac caaaaaccct gcttatcagc tgtttctgag
     9781 ttaagcggaa tcaacctttc tcgccgtcaa aataatccgg agcgatttcg tgggcataac
     9841 aggcgggcga gctatatggt cccggttcgt actcctgtcc atggcgggcc agcagttcga
     9901 ttttgccgct gcctcgctca tacaccccgc gctcggtgac gatgaagtcc atcgggaggt
     9961 cgtaagcctg cgggtggatg gtggcaagcc ttgccagttc ataagttacg ccgattacca
    10021 gcggtttttt attcaagctt gccagcgtgc ggtcgaaata accgccgccg taacccaggc
    10081 ggtagccttc ttcatcaaag ccgttcatcg gcaggagtac cgtatccggc acgagttcgg
    10141 aggattgcgc cggataggga atgtcgagga cgccgtttgc catttttacg ccgggatgcc
    10201 attcgcggaa aataagcggc gtcttaggcg cgacgacgac aggaagtgcc gtgactgcgc
    10261 cccgctcgcg cagtttgcgc atcaggaagc gcgcatcgaa ttcattttta tgcggccagc
    10321 aaaaaaccac cacgccgcgc gccagattcg gaaacgcgcg ctcgagatga ttgctaatgg
    10381 cgtcgttcca agtgccgcgc tccgcgggat tcgccgcaag gcgccgggca atcagatcgg
    10441 cacgcaattc tttgcgccag gttttgagtt tggctgcgtt cataccttat ttcttgcatc
    10501 cggtcatggt atgctaaatc ctccatgaga caaactatag caggaacccg aatttctttg
    10561 ttcgcgctgt gcgccgcgtt gagcttggcc ttgcctgccc gcgcggacca gggcgatgat
    10621 tttttgaagg cgcgcgcagc ctatgcggcc ggcaacatct cccgcctgga cgaatacgcc
    10681 aagcgcttac agggttatgt gctggaacct tacatcgctt actggcaatt gcgctcgcgg
    10741 ctggaggatg cgccgccgga aacggtacgg gcgtttatct ggcgttacag cgatactctg
    10801 ctggccgacc gcatgcgagc ggaatggctg aaagtgctgg cgcgcaaagg ccggtgggag
    10861 ttgtttgccg ctgaattccc gcccgtgcgc aatgaaagca cggagcttac ctgctatgcg
    10921 ctgcagggac ggctgcgcaa taaggatgac accgcgctca tcgaggcgcg cagcctctgg
    10981 ttttccgcgg aggagcagcc cgacagttgc gcgccgctgt ttcaggagat gtttatccgc
    11041 aacgtgctgg gcatcgagga cgtgtggcag aggctgcgcc tcgccttgga agccggcaac
    11101 ctcggcgccg cgaggcgcat catccaatat cttccggcga agctggcgcc caatccgcgc
    11161 ctattcaaaa gcatcgctaa tcggccggag cgttatctcg ccaaaccgac ctacaatctg
    11221 aagacccggc tcggccacga actcactttg ttcgcgctct accgcctggc gcgcaatgag
    11281 ccgaaacggg cgttgtcctt ctggaacaag ctgacaaacg cgttcgacga agaggaacaa
    11341 caatatgtcc ggggccagct ggcgtaccaa gcggcgctta gtcacgagcc tgacgcgctt
    11401 aactggtttg ccaacgtgcc ggtgaatctg ctcaccgacc agcagcttgg ctggaagacg
    11461 cgtgcggctt tacgcacgct ggactggcag gcggtggtcg acggcatcga agcgatgacg
    11521 gttatcgagg cgcgcgaccc tacttggcgc tactggaaag cgcgggcgct cagggagctg
    11581 ggaaaaaacg ccgagagcaa cgcgattttg caggagctgg caaacgacca ttattactac
    11641 ggtcagttgg ctagcgaaga gctgggcgtg acgcacagcg cacccgccgt tgcgtacaag
    11701 ccgagcgaac aggaattatc cgcaatcgag caattgcctt ccatccagcg cgcgctggcg
    11761 ctgtaccaac tggagctgcg ctacgaaggc aaccccgagt ggcaatgggc aattcgtagt
    11821 ttcgacgacc tgcagctggt ggccgcggcg gaaatagcgc ggcgccacca gttgtatgag
    11881 cgcgccgtct ttaccgcgaa tcaaacactc caggtgcatg atttcggcct gcgttatccc
    11941 gctccctacc gcgagcatgt ccaggaatac gtgcgtcagt ccgagctcga tgaagcctgg
    12001 ctttatggcg tgattcgcca ggaaagccgt ttcgagcgcg acgcgcattc cagcgctggc
    12061 gcccgcgggc tgatgcaact catgccggcc accgccaaat gggtggccag gcgcatcggc
    12121 aagggctacc ggcataccaa ggcgaatgac cttgagacca atctcagtct tggcattttt
    12181 tacttgaaac acattctgga tatttccggc ggccagatgg tgctggcttc cgcggcgtat
    12241 aacgccggac cgacgcgggc acggcaatgg ggcgcggatg tgccgctgga aggcgcgatt
    12301 tacgtggaaa ccattcctat taacgaaacg cgcggctacg tcaaaaaagt gctctccaac
    12361 acactctatt acgccagcat cctcggcaaa cagccgctca cgatgaaaag cttgctcggt
    12421 accatcccgc cgcgcgcgca gagccgttcg ttgcaagacg agaggtagcg ggtgtgaagc
    12481 tggataaagt ttgtgtcgtt ggcggttcag gattcgtagg caggcacatt gtccacctgc
    12541 tggcggcaca agagaaaaaa gtgcgggtga ctacgcgcaa ccgcgagcgc tccaaggagc
    12601 tcatcgttct gcccactgtg gacgtggtgc aggcggatgt gcacgatgat gcacagcttg
    12661 agcagatgct tgagggaatg gacgcggtca tcaatctcgt ggggattctg cacgaggaga
    12721 gaagcggcga tttccagcgc gtgcatgtag agctgccgca gcgcatcgcc agaacatgtg
    12781 agcgcctcgg tataccgcgc ttggtgcaca tgagcgcgct caatgcggat gtgaacggcc
    12841 ccagtgttta tctgcgttcc aagggcgaag ccgaggcggc tctcaagaat cgagccaagg
    12901 caccggcagt cacgatattt cgcccttcgg tgattttcgg gcgtgaagac aagtttctca
    12961 acttgtttgc gaaactgctc aggttttttc ccgtattatt tctcgcctgt cccaacgccc
    13021 gcttccaacc gatttttgtg gaagacgtgg cgcgcgcgtt tgtggacagc ctcgctaggc
    13081 ttgaaacctt cggccaaagc tacgatctgt gcgggccgaa agcgtatacg ctgcgtgaac
    13141 tggtgaaata catggcagtg ctcaccggcc gccatcgcct gattgttggt ttaagcgacc
    13201 ggctgtcgta tttgcaagcc tggagcatgg aattcctgcc ggtcaagctc atgacccgcg
    13261 acaactatta ttcgatgaga atcgacagcg tgtgcgactg tgcttttccg gaaattttcg
    13321 gcttccagcc tgccgcgctg gaggcggcgg tgccgcttta ttttgcgggt ggaacgccgc
    13381 gcgggcggta tcggtggttc cgctacaagg cggggcgttg aggcatgagc cgggctactc
    13441 tgtaaaggcc aatgaaaatt tatacagtcg gtggcgcagt gcgcgatgag cttttgggcc
    13501 ttaaagtgac ggatcgagat tacgtagtgg tgggaagcac gccgcaagaa atgcagcgcc
    13561 tgggcttcaa gccggtgggc aaggattttc cggtgtttct gcacccgaaa acgcaccagg
    13621 aatacgcgct cgcccgcacc gagcgcaaaa ccgcgcgcgg ctacagaggt ttcaggattt
    13681 atagcgcgcc ggatgtgacg ctggagcagg atttggcgcg gcgcgacctc accctcaacg
    13741 ccattgccag ggatgagcac ggcaaaatca ttgatcctta cgggggcgtc aaggatttgc
    13801 aggccggcgt gttgcgccat gtcagcccgg cttttgccga agacccagtg cgcatcctgc
    13861 gcgtggcgcg ttttgccgcg cgctacggtt tcaaagtggc cggggaaacc atggctttga
    13921 tgcgccagat ggtgaaaaac ggcgaagtgg atgcattggt ggcggaacgc gtgtggcagg
    13981 agctttcaaa gggtctgatg gaaaagaaac cctcgcgcat gttcgaagtg ctgcgcgagt
    14041 gcggggcgct cgccaaaatc ctgccggaag tggaccggct gtttggtgtg ccccaggcga
    14101 aagagcagca tccggaagtg gataccggca ggcacatcat gctggtgatc gactatgccg
    14161 ccgcaaaaaa tttttcgctg ccggtgcgct ttgccgcgct cacccacgat ttgggcaagg
    14221 gggtaacgcc gcaaaaagat tggccgcgcc atcccggcca tgaaacgaag agcgtggaat
    14281 tggtgaaagc attatgcaag cgcatcaggg ttcccgctgc ctgccgcgat ctggcggtga
    14341 ttacggcgcg ccatcatggc gaagcgcacc gtgcgctcga gcttgatgcg gcaagcctcg
    14401 tcaagctgtt gcaggcaacc gacgctttgc gcaagccgca gcgttttgcc gagttcctgg
    14461 atgcctgcgg ctgtgatttt cacggccgtc ccggttacca gaccagggtt tttgcgcagg
    14521 ccgcccacct gcaaagcgcg ttgcgcgtgg caaaaggcgt ggacgccggc aaaatcgcca
    14581 aacggcttaa agattccagg caaatcgcgg ccaggatacg catagcgcgt accgccgcgg
    14641 tcgagacttg gcttgccggc gaacggattg tgtaaactga tagcccaccg aaacgtttat
    14701 ccagtgcccc attactgaat agaggagaac atcatgctta aacccgtttt gattgcctcg
    14761 ttgttgctgg ttcccgcgtt cgcgctcgcc gatgacgccg gaaagcccgg cgagaaaaag
    14821 caccggatgg aagagtactt caaaaaagcc gacaaggaca acgacggcac gctgaccagg
    14881 gacgaagcca aagccatgcc gcatgtcgcc aagcatttcg atgaaattga cgccgacaag
    14941 gacggcaccg tttccatgga tgaaattcgc gcgtttgcca aggcgcagca taaagacgcg
    15001 catcaccgcg gcgaggaacg cttcaagaaa gccgataagg acaacgacgg cacgctgacc
    15061 aaggacgaag ccaaggccat gccgaatgtc tacaagaact tcgatgctat agacaccgac
    15121 aaggatggca cggtcacgct ggatgaaatc cacaactaca tgaagtcccg gcgggaaacg
    15181 cgccaaggcc agccgaaggc gtattaagcc gcatattgca acgctggtgg gcccaccggc
    15241 gttttttatt ttttaaagcc gggaatcctg ttgcccgctg gatgtcagaa cgcggcaacc
    15301 gatggttccg caaaaatcat ggcacaggat tgaaaatgat agacctttat acctggtcca
    15361 cgcccaacgg ccgcaaaatt tccatcatgc tggaagaaac cggcctgccc tatcgcgcgc
    15421 acccggttga tatcaccaag agcgagcagt tccagccgga atatgtggcg atttccccga
    15481 acagcaagat tccggcgatt gtggatgagc aaggaccaga gggtgtgcca ttcaagctgt
    15541 tcgaatccgg cgcgatcctg atttacctcg cggagaaaac cggaaagttc ttaccgcggg
    15601 cggttttgcc gcgttacgag gtgttacagt ggctgatgtt ccagatgggc aatgtcggcc
    15661 cgtttttcgg tcaggcgcac cactttctgc gtttctccaa ggaaaaaata ccgtatgcca
    15721 tcgagcgcta cagcagggaa accgcgcggc tctacggcgt gcttaaccgg caattggcgg
    15781 gccgcgaata tcttgcggat gagtattcaa ttgccgatat cgcgactttt ccctgggtgg
    15841 cgcgctatga gtggcagcaa atccggcttg aggagtaccc caacgtcaag cgctggtttg
    15901 acgccatcgc ggcgcggccg gccgtcaagc gcggcatggc ggtgcccatg tgacacagtt
    15961 cactgtgcag cactcggtta atcacttaaa atacgcaacg ctttaatcga tgatggccaa
    16021 tccggcaatg ggacttctcg ttcctataca aatcggcaag tatccggtga tccgcgagct
    16081 ggggcgcggc atgaccggcc gtgtctatct ggcgcgtgac ccctttggcg ggcgcgaggt
    16141 ggccatcaag ctggtgagca ccgaagacat tggcaacccc gaggaccgca aggttttccg
    16201 taaagctttc ctgaacgagg catcgcttgc cggcaagctt tcgcatccac acatcgtcgg
    16261 cattctcgac gcggtggtgg aagaagcgat gagctacatc gtgatggaat atatcgacgg
    16321 cagcactctc gaccagtact gcagcgtgga taatctgctg ccgctggaga aagtcgtgga
    16381 aatcaccttt aaatccagcc gcgcgctgga gttcgccaat cacgccgggg taatccaccg
    16441 cgacatcaag ccggccaata tcctgatgca aagcaatggt gacatcaaaa tcagcgattt
    16501 cggagcggcg ctcctgcacg atgcggaaga aacgcaatta accggcgtcg gttcgcccgc
    16561 ttacatgtcg cccgagcagc tgcgcgaaca gccgctcacg cttcaaaccg atatttattc
    16621 gctgggggtg gtgatgtacc ggttgctcac cgggaagttt cccttcaacg ccgcgagcaa
    16681 ggcgagcctg atttaccaga tcctcaacat tgatgtactt ccgcccagcg tgcaccggag
    16741 caatcttcct gcgcaactcg acagcatcgt gttatgcgcg ctgcaaaagg acttgagctt
    16801 gcgctatcgg aactggaacg aattcggcag ggatctcacc ggcgcgttca agaacctggt
    16861 catgccgggt gaaaacatcc cggaaaccga aaaattcgac accataaaaa gcctgccgtt
    16921 tttccgcgat tttcacgacg tgcagatctg ggaagcgctg cgcattacgg cctggaaaaa
    16981 actgccaaaa ggaacggtga tcatccgcga aggcgaccag ggcgactgtt tctacgtcct
    17041 cgccgaaggc gaagtcacgg tcagcaagca gggcaaaccc ctcaacgccc tcaagcgcgg
    17101 cgactgtttc ggggaaatgc tgtactttcg cgccaccacc gcgcaacgca aaacttcggt
    17161 gattgcctcg accccggtca ccgtggttga aatcaaagcc tcgtcgttga cccaggcttc
    17221 gaatccctgc cagaatcagt tcaacaaggc gttcctctcc atccttatcg agcggcacgg
    17281 ctaaggaact tctgattaag tcacacgcgg ttgcgacggt gtgcaatccg ggtgcagagc
    17341 gaggcgcgag gagcgccgtt tggtagaggc taaaccagcg acgaacaact cagaggttcc
    17401 taaggccgac tgtggcgctt taagaattca aggatgtcct tgaccttgag cgggtgggga
    17461 ttcaggccct tgcccttgat cacgcccacc cacgccatgt tcttctgcat ccgctccacc
    17521 acacccggcc actccgtggc ggtatggcgc ttgggatcgg gcagctcatg gcattgtatg
    17581 caggcctcgg tgtaagtccg acccgccgcg gtggtcttga gatcaggata gcgcccgggg
    17641 tctatcgctt tctgcccgta tttactcaag taggcaagca ggttctcgac ttccgcctcc
    17701 gacggcgttt ccatgtcttc catcagttcc ttcatcacca cacccagatt gccttcccct
    17761 tgcatgcgcc gcaccatgcg ctcgactacc acttcccagc gctccggagt gtgcatgtag
    17821 ggactgggca gataatggca ctgcacgcaa tagcgggtga gcgtccgcgc tcccgccgag
    17881 gctggttgcg gcaactcttc gggtttgggt ccgggcggca ggatacgcag tagcatcagg
    17941 ccatgcggtc cctgttccca tttggctggc cctgcggtct ttacgctttc ctgttgctgc
    18001 gccagcgccg cacccgagac agcaaaaaac aggaccaagc aataaagttt cagcctcata
    18061 cggtttggaa atttgctgat gcgctcctgg gattatattg tgttaacctg caattagacc
    18121 agccttcact gaatcagaaa ggagcgcaca tgtcatcgat tgcccgttac catgccctca
    18181 tcatcggaac cggccaatcg ggcaagcctc tcgccgggac gctcgccagg gccggctgga
    18241 agaccgcagt gatcgaacgc gagcatgtgg gcggcacctg cgtcaacgtc ggctgcacgc
    18301 cgaccaagac catggtggcg agcgcgcgcg tcgcgtatct ggcgcgctgc gccgcaaatt
    18361 acggcgtcaa caccggggac gtgagcgtgg atatggccaa agtttacgcg cgcaaaaaag
    18421 ccatagtgga aagtttccgc aaaggcggac aggaccggct ggagagcacg cccaatctcg
    18481 agctcatttt cggcgtggcg cgattcaagg atcaccgcac ggtggaggtg cggttgaacg
    18541 gcggcggaac gagggaaatg actgcggaca gaatcgtcat caacaccggc ggcacccccg
    18601 cgctgccgcc gctacccgga ttgagcgaag cccggcccct gaattcttcc accatcatgg
    18661 agctgcaggc gttgcccgag catctgctgg tgctgggtgg aagctacatc ggcctcgagt
    18721 tcgcgcagat gttccgccgc ttcggcagcc gcgtcaccat cattcatcgt gatgaccggc
    18781 tgatcaaccg cgaagacccg gacgtatccg aggaagtgca ggagatcctg cgccaggacg
    18841 gtctcgagat tttgctcgat gcgcaagtcg tgcgtatttc caggcagcca aacggccgga
    18901 ccgaggtgaa catcaagacg ccggcgggag ag
//
LOCUS       DATIOW010000135         2690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1689685, whole genome shotgun sequence.
ACCESSION   DATIOW010000135 DATIOW010000000
VERSION     DATIOW010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689685"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VLL03_04445"
     CDS             <1..297
                     /locus_tag="VLL03_04445"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HSS46649.1"
                     /translation="GLMFRKHLRGNHGMLFVFPQPGVYGMWMKNTSVPLSVAFLDDQG
                     VIINITDMVPFSEEQHHAAAPAKFALEMPIGWFSKREIKPGAKVEGLSQAPRGE"
     gene            complement(340..828)
                     /locus_tag="VLL03_04450"
     CDS             complement(340..828)
                     /locus_tag="VLL03_04450"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HSS46650.1"
                     /translation="MEYVPALARWVHFLAGVMWIGLLYYFNFVQADAMKAAQADGTAA
                     GISKHVAPRALLYFRWSALVTWLAGAALLGGNFFLAFGLQGVYSVIGVGAWLGTIMLF
                     NVWVLIWPNQKKILGIVQATDEEKNKARRTAFLASRTNTLLSIPMLFFMAAGAHTAMF
                     FG"
     gene            1122..2171
                     /locus_tag="VLL03_04455"
     CDS             1122..2171
                     /locus_tag="VLL03_04455"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSS46651.1"
                     /translation="MSAYLDPALKFMLWVGLAVLGVTLLLLLHTLLLRARLMRRQRHE
                     RQFVEKWRPLLMQGMTEVPKFLPQVPQSEWHIFLSLWIHFQDTSHGESRLKLNQLAWT
                     CGMDHAARQLLDHRSVRTRMTAVTTLGYLQEKNAWARLKNMVNDPHPLLSLASARALT
                     QIDAKAALPELLPLFTRRHDWPLNKITAILNEAGPEAASAPLTQIVESAAPEQFPRLV
                     RLLDCAYDEQALPALRRIMLAATDDRVISACLQALRDPRDAGFARAHVGHHSWFVRVQ
                     AVSALGRLGTRDDHNLLIAPLSDPVWWVRYRAAQALAGLPSITLDEVRQIRENSSDRF
                     ARDILAQVVAEKRPA"
     gene            2168..>2690
                     /locus_tag="VLL03_04460"
     CDS             2168..>2690
                     /locus_tag="VLL03_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSS46652.1"
                     /translation="MSAADWIIILQWFFLLYFIGFNCGYVLLNLLSIGRLKNYMEAKS
                     LGDLPRVYSGYEPPISVLVPAYNEEATIAGLVRSLLQLDYPEFEIIVINDGSKDNTLA
                     MLQREFLLVVFPEAYWQRIKGPGVRAIYRSSAHPGLRVIDKENGGKADALNAGINASR
                     YPLFCAVDTDSILQ"
BASE COUNT          553 a          808 c          785 g          544 t
ORIGIN      
        1 ggattaatgt tccgcaagca tctgcgcggt aaccacggca tgctgtttgt ttttccccag
       61 cccggcgtgt acggcatgtg gatgaaaaac acttccgtcc ccttgagcgt ggcttttctg
      121 gacgatcagg gcgtcatcat caatatcacc gacatggtcc ccttcagcga ggagcagcat
      181 cacgccgccg cgcccgccaa gtttgcgctg gaaatgccca tcgggtggtt cagcaaacgg
      241 gaaatcaagc ccggcgcgaa agtcgaaggc ctgtcgcaag ccccgcgcgg agaataaaaa
      301 agcccgcctg gaacggcggg ctttagaata attaagcgtc tagccgaaga acatcgcggt
      361 atgcgcgccc gccgccatga agaacagcat cgggatggag agcagggtgt tggtgcgcga
      421 agccaggaat gccgtgcggc gcgccttgtt tttctcttcg tcggtcgcct gcacgatgcc
      481 cagtatcttc ttctgattgg gccagatcag cacccacacg ttgaacagca tgatggtgcc
      541 aagccacgct ccgacgccga tcaccgagta aacgccttgc agcccgaacg ccaggaaaaa
      601 gttgccgcca agcagcgccg cgccggcgag ccaggtcacg agcgcgctcc agcggaaata
      661 aagcagcgcg cgcggagcga cgtgcttgct gatgcccgcc gcggtgccgt ccgcttgcgc
      721 ggctttcatc gcgtccgcct gtacgaagtt gaaataataa agcaggccga tccacatcac
      781 acccgcgagg aaatgcaccc agcgggcaag tgccggtacg tattccatgt ctagcctccc
      841 aggaaatgct taacgatgtt gtacagaatc agggtcagga cgaggccgct gatgaccgtt
      901 ccccacagcg aatccaatgg atttttcatg atcgctcctc gagtaaacca gcgaaacttc
      961 gagggacagt ctataacaaa cctgcttact tgggcaatgg caagcgcggc gatccgcttt
     1021 tgacagccga ttttttgaca aataatcaac cgttccgggt tacacttaac catcctggat
     1081 aaagagcacc aaacgcttgc gaaaaggaac ttaagttggg cttgagcgct tatttggatc
     1141 cggctttgaa attcatgctg tgggtcggtc ttgccgtgct gggggtcact ttgcttctgc
     1201 tgctgcacac gttgctgctg cgcgcccgtt tgatgcggcg acagcgccac gagcgccaat
     1261 tcgtcgaaaa atggcggccg ctgctgatgc agggcatgac cgaggttccc aaatttctgc
     1321 cccaggttcc ccaatcggag tggcacatct tcctcagcct gtggatccac tttcaggata
     1381 cgtcccatgg agaatccaga ctcaagctca accagctggc gtggacttgc gggatggacc
     1441 acgcggcgcg gcaactgctc gaccacagga gtgtgcggac acgcatgacg gcggtcacca
     1501 ccttgggcta tttacaggaa aaaaacgcat gggcaaggct gaaaaacatg gtcaacgatc
     1561 cacacccgct gctgtcgctg gcatcggcac gtgcgctcac gcaaatcgat gcaaaagcag
     1621 cgctgcccga gcttctcccg ctctttaccc gccgccatga ttggccgctc aacaagatta
     1681 cggctatatt gaacgaagcc ggaccggagg cggcatccgc gccgctcacc caaatcgtcg
     1741 agtccgccgc gccggaacaa ttcccgcggc tggtgcgcct gctggattgc gcgtacgacg
     1801 agcaagcgct gccggcgcta cgccgcatca tgctcgccgc caccgatgac agggtgattt
     1861 ccgcctgcct gcaggcgctg cgcgatccgc gcgacgctgg attcgcgcgc gctcatgtgg
     1921 gtcatcacag ctggtttgtg cgggtacagg cggtcagcgc cctggggcgg ctgggcaccc
     1981 gggacgacca caacctgctg attgcgccgc tctccgatcc ggtgtggtgg gtgcgctatc
     2041 gcgcggcgca ggcgctggcg ggtcttcctt ccatcaccct cgatgaagtg cggcaaatcc
     2101 gcgaaaattc atcggaccgc ttcgcccggg acatcctcgc tcaggtcgtt gcggagaaac
     2161 ggccggcatg agcgccgccg actggatcat catcctgcag tggttttttc tgctgtattt
     2221 catcggcttc aactgcggct acgtcctgct gaacctgctg tccatcggca ggctgaaaaa
     2281 ctacatggag gcgaagagtc ttggcgattt gcctcgcgtt tattccggct acgagccgcc
     2341 catcagcgtg ctcgtccccg cttacaatga agaagcgacg attgcgggtc tggtgcgctc
     2401 gctgctgcag ctcgattatc cggaattcga aatcatcgtt atcaacgacg gttcgaaaga
     2461 taatacgctg gcgatgctgc agcgcgagtt tttgctggtg gtgtttccgg aagcgtattg
     2521 gcagcgcatc aagggcccgg gcgtgcgcgc catttaccgt tcttcggccc atcccggcct
     2581 gcgggtgatc gacaaggaaa acggcggcaa ggcggatgcg ttgaacgccg gcatcaacgc
     2641 ctcgcgttat cctttgttct gcgcggtgga cacggattca attctgcagc
//
LOCUS       DATIOW010000136         2176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_776273, whole genome shotgun sequence.
ACCESSION   DATIOW010000136 DATIOW010000000
VERSION     DATIOW010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776273"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /gene="pcnB"
                     /locus_tag="VLL03_04465"
     CDS             <1..1272
                     /gene="pcnB"
                     /locus_tag="VLL03_04465"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HSS46653.1"
                     /translation="IKKQQISPGALKTVVTLKEKSFAAFVVGGAVRDLLFGLAPKDFD
                     VATDATPEQVRAAFRRAHIIGRRFRLVHVMHGNETVEVSTFRGPQAATENAPQDAHGR
                     ILRDNVFGDQQQDAERRDFSINALFYDPITEEVMDYQGGFADLKARRLRIIGDAESRY
                     REDPVRMLRAVRLAAKLGLEIEPATRIPIPRLAPLLQNVPASRLFEEMLKLLLSGHAA
                     ESVKALRVSGLHHGLFPMLDIIVEQPLGQRFVMLALKNTDERIRQDKPVSPGFLFAAL
                     LWHEVLAGWKSLQSQGVHLMQALHQAMEQVIRLQAERLAIPKRFSAVMKEIWSMQPRF
                     LNRAGKRPFRLLEHPRFRAAYDFLLLRCDNTEVEAELGKWWTAFLNADNEQRRSMLLA
                     DGTPRKRKHRSRRRRPVMGNAAGGYDGGTAS"
     gene            1269..1760
                     /gene="folK"
                     /locus_tag="VLL03_04470"
     CDS             1269..1760
                     /gene="folK"
                     /locus_tag="VLL03_04470"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HSS46654.1"
                     /translation="MNTETAFVALGSNLEAPLTQIRNAFAELGALPKTRLIARSSLYR
                     SAPVGNVNQPDFINAMAKLETALTPRELLAALLEIERRHGRRRDFPNAPRTLDLDLLL
                     YGDLIHHEHGLTIPHPRMHQRAFVLSPLHEIAPDCVVPGIGSVADLLATCTGQQLKRL
                     LED"
     gene            1764..>2176
                     /locus_tag="VLL03_04475"
     CDS             1764..>2176
                     /locus_tag="VLL03_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HSS46655.1"
                     /translation="MLPDKFRYIVVEGPIGVGKTSLARLLSERFDGQPLLENPDANPF
                     LPKFYQDIPRYAFSTQLFFLFHRVSQLQGLAQLDMFDRLTIADFILDKDPLFARLTLN
                     DDEFRLYHEIYRHFQPQAPRPDLVIYLQASVETLI"
BASE COUNT          450 a          631 c          636 g          459 t
ORIGIN      
        1 atcaaaaagc agcagatcag cccgggcgca ctgaaaacgg tcgttacgct caaggaaaaa
       61 agtttcgccg catttgtggt gggcggcgca gtgcgcgatt tgctgttcgg ccttgcgccc
      121 aaggatttcg atgtcgccac cgacgccaca ccggagcagg tgcgcgccgc tttccgccgc
      181 gcgcatatca tagggcggcg cttccgtctg gtgcatgtga tgcacggcaa cgagacggtc
      241 gaggtttcca cctttcgcgg gccgcaggcc gcaaccgaaa acgcgccgca ggatgcgcac
      301 ggcaggattt tgcgcgataa cgtgttcggt gatcagcagc aggatgcgga gcggcgcgat
      361 ttcagcatca atgcgctatt ctacgatccg ataaccgaag aagtcatgga ttaccaggga
      421 ggctttgccg atctcaaggc gcggcggctg cgcatcatcg gcgatgcgga gagccgctac
      481 cgtgaagatc cggtgcgcat gctgcgcgcg gtgcgcttgg ccgccaagct ggggctggaa
      541 atcgaaccgg cgacacgcat acctattccc aggctcgcgc cgcttttgca aaacgtgccg
      601 gcgtcgcggc tgttcgagga aatgctgaaa ctcctgcttt ccggacacgc cgccgagtcc
      661 gtcaaagcat tgcgagtaag tggcttgcat cacggcctct ttcccatgct tgacataatc
      721 gtcgaacagc ctctcggtca gcgtttcgtg atgctggctc tcaagaatac cgacgagcgc
      781 atccgccaag acaagccggt atcgcccggc ttcctgtttg cagccttgct gtggcatgaa
      841 gtgttggctg ggtggaaaag ccttcaaagc cagggagtcc atctcatgca ggcgctacat
      901 caggcgatgg agcaggtgat acgcctgcag gcggaaaggc tcgccattcc caaacgtttt
      961 agcgcggtca tgaaggaaat ctggtccatg caaccgcgtt ttttaaaccg cgcgggcaaa
     1021 cgcccgttcc ggctgcttga acatccgcgc ttccgcgccg cttacgattt tctgctgctg
     1081 cgctgtgaca acactgaagt cgaggcagaa ctcggaaaat ggtggacggc gtttctaaat
     1141 gccgataatg aacagcgccg aagcatgctg cttgccgatg ggacgccgag aaaacgcaag
     1201 caccgcagca gaagaaggag acccgtcatg ggtaacgccg cagggggtta tgatggggga
     1261 actgcgtcat gaacacggaa accgcatttg tcgctctggg cagcaatctc gaggctccgc
     1321 tcacgcaaat acgcaacgct tttgccgagc tcggcgcgct gccaaagacc aggctgattg
     1381 cgcgttcttc actttaccgc agcgccccgg ttggcaatgt aaatcaaccg gattttatca
     1441 atgccatggc caagctcgaa accgcgctta ctccgcgcga gctgctcgcg gcgctcctgg
     1501 aaatcgagcg gcgccacggc cggcggcgcg actttcccaa cgcgccacgc acgctggacc
     1561 tggatttgct gttatacggt gatctgatcc atcacgagca cggtttgacc attccacacc
     1621 cgcgcatgca ccagcgcgca ttcgtattgt cgccgctgca tgaaattgcg ccggattgcg
     1681 tggtccccgg catagggtcc gtggccgact tgctcgccac ctgcaccggc cagcaattga
     1741 agaggttgtt ggaagactga accatgttgc ccgataagtt tcgttatatt gtcgtcgagg
     1801 gcccgattgg agtggggaaa actagtctgg cacggctttt atcggagcgc tttgacggcc
     1861 agccgctact cgaaaatccg gacgccaatc ctttcctgcc caagttttat caggacatcc
     1921 cgcgctatgc gttttccacg cagctgtttt tcctgtttca ccgcgtgagc cagttgcagg
     1981 gcctggcgca attggacatg tttgacaggt tgaccatcgc cgatttcata ctcgacaagg
     2041 acccgttgtt cgcgagactt actttgaatg acgatgaatt ccgcctgtat catgaaatct
     2101 accggcattt tcagccgcag gcgccgcggc ctgacttggt gatttatctg caggcgtcgg
     2161 tggaaacgct gattga
//
LOCUS       DATIOW010000137        11823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_776275, whole genome shotgun sequence.
ACCESSION   DATIOW010000137 DATIOW010000000
VERSION     DATIOW010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11823
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776275"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..520)
                     /locus_tag="VLL03_04480"
     CDS             complement(122..520)
                     /locus_tag="VLL03_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HSS46656.1"
                     /translation="MTPKIILPYAALLGLLLVYLSYKVVRYRVKFKIGVGDGGNAELV
                     RAIRVQGNFVEYVPLALLLISLVELAGFSVWVVQVLGIVLLVGRIIHAQGLGASAGPS
                     RARVIGTASSWLTIAVAAILCLLAFGGVIF"
     gene            complement(535..1257)
                     /locus_tag="VLL03_04485"
     CDS             complement(535..1257)
                     /locus_tag="VLL03_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSS46657.1"
                     /translation="MAKPEVAVVVGVGPGLGSALARRLAHAGMHVAAASRDAAKLVSL
                     TRELSSQGVKGRAYDCDATDEESVKKLFSKVAADLGKPGLVVYNAGAFVRKGFLETTK
                     EEFERCWKVGCLGGFLVGQAAAREMLSAENPSGTIIFTGATASLRGGALFHNLAVGKF
                     GLRALAQSMARELQPKGIHVAHVVIDSQIRSDRTGYREEGRGKDAVLDPNAIAETYYQ
                     LHLQPRSAWSLEVDLRPWVEKF"
     gene            1360..1527
                     /locus_tag="VLL03_04490"
     CDS             1360..1527
                     /locus_tag="VLL03_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46658.1"
                     /translation="MKIHQDLDARYSVAIHWGTFPLPDVALDQPPRDLVKARLEAGLT
                     AERCDEAPEDT"
     gene            1786..2443
                     /locus_tag="VLL03_04495"
                     /pseudo
     CDS             1786..2443
                     /locus_tag="VLL03_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
     gene            complement(2477..3295)
                     /locus_tag="VLL03_04500"
     CDS             complement(2477..3295)
                     /locus_tag="VLL03_04500"
                     /inference="COORDINATES: protein motif:HMM:NF004855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HSS46659.1"
                     /translation="MTAACARNQTDIVQQEVSMRIEFNLPGPPKFGSIAEQRRHIKQR
                     LAGAFRLFSKFGFDEGVAGHITARDPEFSDRFWVNPFGVYFGQIKASDMILVNHKGEV
                     VEGNRPVNAAAFAIHSQVHAARPDCMAAAHAHSMYGKTWSMLGRLLDPLTQDACAFYQ
                     DHALFDDYTGVVLDTEEGKRIAQTLGNRKAIILRNHGLLTVGKTVDEAVWWFITMERS
                     CQAQLMAEAAGKPILISEGNARLTYTQIGSEFAGWFQFQPLWESIIQEQPDLLD"
     gene            complement(3295..3987)
                     /locus_tag="VLL03_04505"
     CDS             complement(3295..3987)
                     /locus_tag="VLL03_04505"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HSS46660.1"
                     /translation="MPKLELYSAPVCPFAQRTRLALLEKHIEFTLSEIDLNHKPEDFG
                     KISPYSKIPVLKRGDDLVWESAIINEYLEEVYPQPALMPLEPGKRALARFWIDFCNVR
                     FVPTFYKLLLAQSGDKQNAHREEMLRHLRFLENQALGKNPDGLYFHGKELTLTDLTFY
                     PFFERFCVLKHYRQCDIPADCARLKRWLEHMRGRASVKAAQNDEKFYLRSYAKYADGS
                     ASGVTAQEMRSA"
     gene            complement(3996..4796)
                     /locus_tag="VLL03_04510"
     CDS             complement(3996..4796)
                     /locus_tag="VLL03_04510"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HSS46661.1"
                     /translation="MQGKTVALLEARVSDQLAELVVKHGGKPFSAPALAEVPDVDSEY
                     IGRLIREWEAQPVKVVIFQTGVGTRALFNAADGLGLTGTLMHLLSQSTIVVRGPKPTA
                     VLRSRNVRIDLSAEEPYTTAEVLDALDAVPIRGERVVVQRYGETNVELEKALQLRGAT
                     VIEIPTYRWALPENTQPLVDLIGALERGEIHAVAFTSASQAYNLFAVAEQLGKKDALK
                     ANLNKTLVASIGPVCSRALSTLGVKVGLETRPPKLGPFITALAQALSK"
     gene            4894..5148
                     /locus_tag="VLL03_04515"
     CDS             4894..5148
                     /locus_tag="VLL03_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HSS46662.1"
                     /translation="MVFDNILGFVAGILTTLAFVPQAWRVWKTRSVKDISLGMYIIFT
                     SGVDLWLVYGIVIHSLPLILANSVTFVLAAFILIIKIRLG"
     gene            5571..5930
                     /locus_tag="VLL03_04520"
     CDS             5571..5930
                     /locus_tag="VLL03_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HSS46663.1"
                     /translation="MRGDAGIKVTLNGIQEVPPVDTAASGSGKILVAADKSVSGSITT
                     SGVAGAAAHIHQGAAGTNGPVIIGLVKTGDNVWTVPAGAKFTDAQYTSFLAGNMYINV
                     HSAAHKNGEIRGQLNPK"
     gene            6123..6326
                     /locus_tag="VLL03_04525"
     CDS             6123..6326
                     /locus_tag="VLL03_04525"
                     /inference="COORDINATES: protein motif:HMM:NF039498.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5676 family membrane protein"
                     /protein_id="HSS46664.1"
                     /translation="MCCGTNGGIDFLNALFHGLDFHKLGTPMTFTFPMLLYPLVVFVI
                     WSFAAGMLYTWFHNLLHSNSRQS"
     gene            6622..8751
                     /locus_tag="VLL03_04530"
     CDS             6622..8751
                     /locus_tag="VLL03_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HSS46665.1"
                     /translation="MIAPGTIYTCPMHPEIRQVGPGACPKCGMALEPVVASAESQPNP
                     ELVDMTRRFRTGLVLTVPVLVLAMVEHLPDMHLEHYVSPMVSGWIQFILSSPVVLWAG
                     WPFFVRGWNSLVSRSLNMYTLIGLGVAVAFGYSAVAFLVPGIFPPAFRDSSGRVGLYF
                     EAAAVIAVLVLLGEVLQLRARANTSSAIRALLKLAPATAVRVRADGTDEEVPLDQVQK
                     GDRLRVHPGDKVPVDGIVLEGHSSVDESLVTGEPVPVEKHSGEKVTGGAINGTGSFVM
                     RADRVGSETLLAQIVQMVVEAQRTRAPIQNLADVVSSYFVPTVVGIAIIAFIVWSIYG
                     PPPAMAFALVSAVSVLIIACPCALGLATPMSIMVGTGRGAQAGVLIRNAEALERMERI
                     DTLVVDKTGTLTEGKPKLTSVVPAEGVAENQVLQVAASLERGSEHPLAAAILTGAKER
                     GVTPLPVENFNAVTGKGVQATLESRSVALGNLRLAESLVEVPQTYKERADALRKNGET
                     VVFLVDGERFAGLIGVADPIKASTPEALRALAAEGIHVVMLTGDNRVTAQAVAQRLGI
                     TEVRADVLPQEKGAVVKALRAEGRAVAMAGDGVNDAPALAEAEVGIAMGTGADVAIQS
                     AGITLMKGDLRGIVRARLLSRATMRNIRENLFLAFVYNMLGVPVAAGVLFPFTGLLLN
                     PMIAAAAMSASSVSVVANALRLRKARI"
     gene            complement(8892..10160)
                     /locus_tag="VLL03_04535"
     CDS             complement(8892..10160)
                     /locus_tag="VLL03_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HSS46666.1"
                     /translation="MTISSVSFALKQASMLAVATFACAVTLAQAADFKVLQPLGDKPM
                     AVKKGVLPWGGYYVPSTPETVRWGSLPNAEAKAVLSVPSGSVVTFDTVSHEGILEDQG
                     KDPVRYFGQFGIKPAQVLKDARVIAASKLEHDFAKDGPHIVTGPVEIQGAKAGDVLKV
                     EILSQQFRVPYGVVSNRHGKGALPGEFPENKGPEAGADAAHPNLYHNVSVFVPIRQIS
                     GKWYAIIKDKNGVVARFPLQPFNGLFGVAVNTKDKPNSVPPGEYAGNMDLNELTTGAT
                     LYIPIQVDGALFYAADPHFVQGDGEVALTAVEGSLRSTFRLTVLKPGDASLPMKAPMK
                     NPFAENSKYWIPIGLNTDLNEAMKDAVRQTIQFLNLKLGMDRASAIAYASAAVDFEVT
                     QVVDKVKGVNGMIRKSDFQSGSRAKKGDAE"
     gene            complement(10315..10390)
                     /locus_tag="VLL03_04540"
     tRNA            complement(10315..10390)
                     /locus_tag="VLL03_04540"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10354..10356),aa:Glu,seq:ttc)
     gene            complement(10411..10486)
                     /locus_tag="VLL03_04545"
     tRNA            complement(10411..10486)
                     /locus_tag="VLL03_04545"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10451..10453),aa:Ala,seq:ggc)
     gene            complement(10542..11822)
                     /gene="gltX"
                     /locus_tag="VLL03_04550"
     CDS             complement(10542..11822)
                     /gene="gltX"
                     /locus_tag="VLL03_04550"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HSS46667.1"
                     /translation="MRIEDTDRERSTRESIQAILDGMSWLKLNWDEGPFYQMQRLPRY
                     QEVAQQLLKEDKAYYCYASREELEVMRKAQRARGEKPRYDGRWRDSKQMPPAGVTPVV
                     RFKNPLDGEVEFDDLVKGRIVVQNSELDDVVILRADGVPTYNFGVVVDDLDMGITHVI
                     RGDDHVNNTPRQINILKALGTPLPRYAHVPMILGTDGERFSKRHGAVSVMQYKEEGYL
                     PEALINYLARLGWSHGDAEMFSIEQFVSWFDLKHISKSPAQFNPEKLLWLNHQYIKSG
                     QDAMLAEQIVPDIKRRGGNIAAGPKVVEVVRLLKDRANTLNELAGVAMLFYRYIPPSK
                     ELIVEHVNGEVIPALREVAERFGTIDWQRLAINEAIKSVVVKHGLKLPLLAMPLRVLV
                     AGSAQTPPIDATLELIGRERALKRLREQLADHAQ"
BASE COUNT         2560 a         3454 c         3278 g         2531 t
ORIGIN      
        1 aagaaactgg aacaggaaat aggcgaaaag cgcgggcgca ctatttagtc tcagaaaaaa
       61 atggtgggga aaatagataa agccatccgc tgcgcgacaa gctcgccaaa ttgcaagagt
      121 attaaaatat tactccgccg aacgccagca aacagagaat tgcggcgacg gctattgtca
      181 accaactcga tgccgtgccg atgacgcgcg ctcgcgaggg gcccgccgaa gcgcccaggc
      241 cttgcgcatg aattatccgc cccaccaaca acacgatgcc cagcacctgc accacccaga
      301 cgctgaagcc ggcaagctcc accaacgaaa tcaacagcag cgcgaggggc acgtattcca
      361 cgaaattgcc ctgcacgcgg atggctcgaa ccaactcggc gttgccgccg tcgccgacac
      421 cgattttgaa tttgacgcga taacgcacta ctttataaga aagataaacc agcaacaggc
      481 ccagcagggc ggcgtatgga agaatgattt tcggggtcat ggctagccct tttctcaaaa
      541 tttctcgacc cagggccgca aatccacttc caggctccag gcgctacggg gttgcaggtg
      601 cagctgataa taggtttcgg caattgcgtt tggatcgagc acggcatctt tgccgcgccc
      661 ttcttcacga tagcccgtcc ggtcggaacg aatttggctg tcaatcacca catgcgcaac
      721 gtgaatgccc ttgggttgca gctcgcgcgc catgctttgc gccagcgccc tcaatccgaa
      781 tttgcccacg gccagattgt gaaacaaggc gccgcctctg aggctggcgg tggcgccggt
      841 gaaaataatc gtccccgacg gattttctgc tgagagcatt tcgcgcgccg ccgcttgtcc
      901 aaccagaaag cccccaaggc agccgacttt ccagcagcgc tcgaattctt ccttcgtggt
      961 ttccagaaat cccttgcgca cgaaagcgcc ggcgttgtac acaaccagac ctggcttgcc
     1021 aagatccgcc gcaaccttgg aaaacagttt tttcacggat tcctcgtccg tagcatcgca
     1081 gtcataagcc cggcctttaa cgccttgaga ggaaagctcc ctggtaagcg ataccagctt
     1141 tgccgcgtcc cgcgaagccg cagctacatg catgccggcg tgcgccaggc gccgcgcgag
     1201 cgctgaaccc aatcccggcc ccacgcctac gacgactgcg acttccggtt tcgccattgc
     1261 ttctccattc aagtaaacta tcgcgctagt ttgccatgct tatgaaacca agaaaactgc
     1321 tgaccatgct gctcggctcc acgctgcccc gaggaagcgg tgaaaattca ccaggacctc
     1381 gacgcccgct attcagtggc catacactgg ggaacattcc cgcttcccga cgtggccttg
     1441 gaccagccgc cgcgcgattt ggttaaagca cgcttggaag cgggtttaac cgcagagcgt
     1501 tgtgatgaag caccggaaga cacgtgacct gagcgggctg atcaaatctt aaatcaccgc
     1561 cagcgcctat tgcagctttc cccggggcga tctacagcag gtcgcgcgtc aactcaacgg
     1621 cgccacggcc attactccgt catcagcctg tgcatcggtt ggagcaagcc ccggtgatgg
     1681 ttattgaaaa ggtataatat ttccaacatg aaaaattcga ccgcgaaatc ttcaatgaaa
     1741 tggggaacga cgcggtaagt ctcgcatcat agatggggga acaccatgtt gaaactgtgc
     1801 ggctttagcg taagcaatta ctacaacaag gtgaagttcg ccttgctgga aaaaacatcc
     1861 cgttccggga agaactcgtc tatcccaaca gcgaagaagc gttcctcaag cgcacgccga
     1921 tgggcaaggt gccgttcatt gaaaccaacg gcggcacgat ttgcgagtcg caggtcatca
     1981 tcgaatatct ggaagatatc taccccactc cgccgcttta ccccaaaaat ccccaccaga
     2041 ccgccaaatg tcgcgagctt atacagttca tcgaattata tctggagctg gtagcgcgcc
     2101 ggctttatgg cgaagtgttc ttcggccgca aggttgccga tgacgtcaag gcagaagtca
     2161 aacagaggtt gggaaaaggc gtggcgacgt ttgccgggct ggttaaattt gctccgtata
     2221 tcgccggcaa tgagtttacc tacgccgact gcgcggcgtt cgtgcatctg ccgatggtag
     2281 cgcatgcctc caagaccatt tacggcaagg atgcgtggga tccgttggca gcgccgcgcc
     2341 ctatctcaag cgggttggtg cgcgcccgca tgcgcaaaaa gtcatgcgcg accgtgaagc
     2401 cgctctgccc gcgttcatgg cgctcatcaa aaaacgaact taaataaacc cgatatcgcc
     2461 gatgccgcgg ccagcttcag tcgagcaagt ccggttgttc ctggatgatg ctttcccaca
     2521 gcggctgaaa ctggaaccag ccggcaaact cgctgccgat ttgcgtgtaa gttaacctgg
     2581 cgtttccctc gctaatcaag atcggcttgc ccgccgcttc cgccatcaat tgcgcctggc
     2641 aggaacgctc catggtgatg aaccaccaca ctgcttcgtc tacggtcttg ccgacagtca
     2701 acaaaccgtg gttgcgcaaa attatcgctt tcctgttccc cagcgtctgc gcaatgcgtt
     2761 tgccctcttc ggtatccagc accacgccgg tgtaatcatc aaacagcgcg tggtcctggt
     2821 aaaacgcgca ggcgtcctga gtgagcggat ccagcaggcg cccgagcatt gaccaggtct
     2881 tgccgtacat ggagtgcgcg tgcgccgccg ccatgcaatc gggccgcgcg gcatgcacct
     2941 gcgaatgaat ggcaaacgca gcagcattca ccggacgatt gccttccact acttcaccct
     3001 tgtgattcac caaaatcatg tcggaagcct tgatctggcc gaaatacacg ccgaagggat
     3061 tcacccagaa gcggtcggaa aattccgggt cgcgcgcggt gatgtgtccc gccacgcctt
     3121 cgtcaaatcc gaacttggaa aacagccgga acgctccggc caggcgttgt ttgatgtgac
     3181 ggcgctgctc cgcgatgctg ccaaacttcg gcggtccggg aagattaaac tcgattctca
     3241 tggaaacctc ctgttgcacg atgtccgttt gattccgcgc gcaagcggcc gtcatcaggc
     3301 gctgcgcatt tcctgagcgg taacgccgga ggccgaaccg tcggcgtatt tggcatagga
     3361 acgaagataa aacttttcgt cgttttgcgc ggctttgacc gaagcacggc cgcgcatgtg
     3421 ctccaaccag cgctttaatc ttgcgcaatc cgcgggaatg tcacactgac gataatgctt
     3481 gagaacgcaa aaacgttcaa agaagggata aaaggtcaaa tccgtaagcg tcagttcctt
     3541 gccgtgaaaa tacagcccgt caggattttt acccagcgcc tgattttcga ggaaacgcag
     3601 gtgcctgagc atctcttcgc ggtgtgcatt ttgtttgtca ccgctttgcg caagcagcaa
     3661 cttgtaaaaa gtcggcacga atctgacgtt gcagaaatca atccagaagc gcgcgagcgc
     3721 ccgctttccc ggttcgagcg gcatgagcgc cggctgcgga taaacctcct ccaagtattc
     3781 gttgatgatt gcggattccc ataccagatc gtcaccgcgc tttaacaccg gaatctttga
     3841 atacggtgag attttgccga aatcttccgg cttgtggttg aggtcgattt cgctgagggt
     3901 aaattcgatg tgcttttcca gcagcgcaag ccgggtgcgc tgggcaaacg ggcaaaccgg
     3961 ggcgctgtac aattcaagtt tgggcatggg agtacttatt tagataaagc ttgggcgagc
     4021 gcagtgataa acggcccgag tttgggcgga cgtgtttcca gcccgacttt tactcccagc
     4081 gtgcttaacg ctctgctgca aaccgggccg atggacgcca ccagagtttt attgagatta
     4141 gctttcaacg catctttttt tccaagctgt tccgccacgg caaaaagatt gtaagcctga
     4201 gaggcgctgg tgaacgccac ggcatgaatc tcgccacgct ccaacgcgcc gatcaaatca
     4261 accagcggct gcgtattttc cggtaacgcc cagcggtaag tggggatttc gatcaccgtc
     4321 gcgcctcgga gttgcagcgc cttttccagc tcgacattgg tctcgccgta gcgctgcacc
     4381 accacccgct cgccgcgaat cggcacggca tcgagcgcat caagaacttc ggcggtggtg
     4441 taaggttctt cagcgctcaa atcgatgcgc acattacgcg agcgcaaaac cgcagtgggc
     4501 ttggggccac gcaccacgat cgtcgattga gaaagcagat gcatcagcgt cccggtcaag
     4561 cccaggccgt cggccgcgtt gaagagcgcc cgggttccaa ccccggtctg aaaaatcacc
     4621 accttgaccg gctgcgcttc ccattcacgt atcagcctgc cgatgtactc actgtcgaca
     4681 tcaggcactt cagcaagcgc cggcgctgaa aacggcttgc cgccgtgctt caccaccaac
     4741 tcggcgagct ggtcgctgac ccgggcttcc aatagcgcaa cggttttgcc ttgcataggt
     4801 tgattgggga tcggttagaa gaatgagttt agagaagaac caagcattgt acaatcacgc
     4861 tcattgagga ttaagtttga taagaactgc taaatggttt ttgataatat tttggggttt
     4921 gttgcaggca ttctcaccac gctggcgttt gtgccgcagg cctggcgagt gtggaaaacg
     4981 cgctcggtaa aggacatttc gctcggcatg tatattatct tcaccagcgg ggtcgacctg
     5041 tggctcgttt atggaatcgt catccattcc ctgccgctga ttcttgccaa cagcgtcacc
     5101 ttcgtgctgg cggcgtttat tttgatcata aagattcgct taggctgaat tttaatatta
     5161 aatatttaaa atcaatatat tattttagtt acttaatgta atgtattaaa tacaaaaacc
     5221 gttgtccggg tggctatgaa tcgcctggag aacgttacaa tgtattagac tattatcaac
     5281 ccggtacaca aaaattgccc ggccggttca agacttgacc cagatcgcaa agtgaactcg
     5341 tggccagatc gattctccaa aaaagcctta cccaaaattc ctcatttgaa gcaagtcaaa
     5401 atgttggaag ggatgccact tctgcttagg cttaattttc ttttaaatta aagaatattc
     5461 ctgtctaaac cttaatgaag cggagataaa aaatgaatct catttctcgg aagctgccca
     5521 tagcgttttt gactgttacc gtaatccttt tcctcgcagc ctgcgccggc atgcgcggag
     5581 acgcggggat caaagtgacg ctcaatggaa ttcaagaagt ccctccggta gatacagctg
     5641 cgtctggaag cggcaagatt cttgttgccg ccgacaaatc cgtgagcggc agcattacca
     5701 cctcaggtgt tgcgggtgcc gccgcacaca tacaccaagg cgcggcaggg accaacggcc
     5761 cggtaattat cggactggtt aagactggcg acaacgtctg gacggttccg gcgggtgcga
     5821 aattcaccga tgcgcaatac acaagttttt tggccggcaa catgtatata aacgttcaca
     5881 gcgcggcaca caaaaacggc gagatacgag gacagcttaa tccaaagtaa acgcaaccga
     5941 aatggtgggc aagcaaacgc accaacgttt gtggcgtccc caaccggatt cgaaccggtg
     6001 ttaccccgtg aaagagcgat atttcacttg atacatcaaa atatcgccca agcttccttg
     6061 gaaaacagga gccccactgg cactgacgct cgcgatcacc tacacagttt gcgcagctgc
     6121 caatgtgctg tggcacgaac gggggaatcg atttcctaaa cgccttgttt cacggtttag
     6181 actttcacaa gctcggaacg cccatgacgt ttaccttccc gatgttgctc tatccgttgg
     6241 tggtcttcgt gatatggagc ttcgcggccg ggatgctcta tacctggttt cataatctcc
     6301 ttcatagcaa cagcagacaa agctgacgca agcggtggct ttggacaatc tttggaacaa
     6361 cgcgatggcg accgaccctg tgtgcggcat gcgggtcgat ccgatcaagt cggccggaag
     6421 cgaaccctat ctaggcaagc agtatttctt ctgctccaag ccttgccagg caaaattcaa
     6481 ggctgacccg gcagcatacg ccagcaagaa gacgggcggt gagcactcgt gcggacacgg
     6541 ccacgcacac ggagccgact gcgtccacga tcacgtacaa cacccgcatc gcgctgctga
     6601 gtcattgcgc cggtcgtgca gatgatagcg cctggtacta tttacacctg cccaatgcat
     6661 ccggagattc ggcaggtcgg gcccggcgcc tgcccgaaat gcggcatggc gctcgagccg
     6721 gtggtggcaa gcgccgaaag ccagccaaac ccggagctag tcgacatgac gcggcgattc
     6781 cggaccggcc tcgtcttgac agtgccggtg ctcgtgctcg ccatggtgga gcatctcccc
     6841 gacatgcatc ttgaacacta cgtcagcccg atggtttcgg gctggatcca gtttatcctg
     6901 agctcgccgg tcgtcctctg ggcgggttgg ccctttttcg tgcgcggctg gaattcgctc
     6961 gtgtcgcgta gcctcaacat gtacacgctc atcggcctcg gggtggcggt tgcctttggc
     7021 tacagcgccg ttgcattcct cgtcccgggt atcttccctc ccgcctttcg ggacagctcg
     7081 gggcgtgtcg ggctctactt cgaggcggcc gcggtaatcg cagtactggt gctgcttggc
     7141 gaagtactgc agttgcgcgc gcgcgcgaac accagcagcg ccatccgcgc cctgctcaag
     7201 ctcgcgccgg cgacggccgt tcgcgtacgt gcggacggaa ccgacgaaga agtgccgctc
     7261 gatcaagtgc agaaaggcga ccgtctgcgc gtgcacccgg gcgataaagt gcccgtcgac
     7321 ggcatagtgc ttgagggaca cagcagcgtc gatgaatccc tggtcacagg cgagccggtg
     7381 ccggtggaaa agcactccgg cgaaaaggtc acgggtggcg ccatcaacgg caccggcagc
     7441 ttcgtcatgc gcgccgatcg tgttggctcg gagacgctgc tcgcccagat tgtgcagatg
     7501 gtcgtggagg cgcagcgcac ccgcgcgccg atccagaatc ttgccgacgt cgtgtcgtcc
     7561 tatttcgttc ccacggtagt gggaatagcc atcatcgcat ttatcgtgtg gtccatatac
     7621 gggccgccgc cggcgatggc gtttgccctg gtaagcgcag tgtcagtgct gatcatcgcc
     7681 tgcccttgcg cgctggggct cgccacgccg atgtccatca tggttggcac cggtcgcggt
     7741 gcgcaggcgg gcgtgctcat ccgtaacgcc gaggcgcttg agcggatgga gaggatcgac
     7801 acgctggtcg tcgacaagac tggcacgttg accgaaggca aacctaagct gacttcggtg
     7861 gtgcccgcag aaggcgttgc ggaaaaccag gtgctgcagg tcgccgcaag tctcgaacgt
     7921 ggcagcgagc atccgctcgc agcagcgatt ctgacgggtg caaaggaacg aggtgtgacg
     7981 ccgctgccgg tcgaaaattt caacgcagtc accggcaagg gtgtgcaggc cacgctcgaa
     8041 agccgcagcg tcgcgctggg taatcttcga cttgccgaat cccttgtcga ggtgccgcag
     8101 acctacaaag agcgcgccga cgccctgagg aagaacggcg agaccgtcgt ctttctcgtg
     8161 gacggcgaac gtttcgcagg gctcatcggc gtagcagatc cgatcaaggc gtcgacgcct
     8221 gaagcgctgc gggcgctcgc tgccgagggg attcatgtcg tgatgctgac cggcgacaac
     8281 cgggtcacgg cgcaggcggt agcccagcgg ctcggcatca ccgaagtccg cgccgacgtc
     8341 ctgccgcagg aaaaaggcgc ggtcgtaaaa gcgctgcgcg ccgaagggcg cgcggtggcg
     8401 atggcgggcg acggcgtcaa cgacgcgccc gcgcttgccg aagcggaagt cgggatcgcc
     8461 atgggcaccg gcgcagatgt cgccatccag agcgccggca tcacgctgat gaaaggcgat
     8521 ctgcgcggca tcgtgcgagc gcggcttctt tcccgcgcca ctatgcgcaa catccgcgag
     8581 aacctgtttc tggccttcgt ctacaacatg ctgggcgtgc cggtcgccgc cggcgtactg
     8641 tttcctttta ccggactgct gctcaatccg atgatcgccg ccgcggcgat gagcgcgagc
     8701 tcggtgtcgg tggtcgcgaa tgcgctcagg ctgcgcaagg cgaggatcta gcgatccgac
     8761 cacagtcgaa atggaacgat gaaatatcac tacgtttggt tgctttggtc gagcgtgttc
     8821 atgctcccta ggggtaggtc aattttccct tcgcctagat ctgatctcgt gtgttgatga
     8881 tcccggcagg gctactcggc gtcacccttt ttcgcgcgcg atccagactg gaaatcggat
     8941 ttccgaatca tgccattcac gcctttgacc ttatcgacca cttgggttac ttcaaagtcc
     9001 accgcagcgc tcgcataggc gatcgcagaa gcgcgatcca tcccgagctt caggttgaga
     9061 aactggatcg tctggcgcac cgcatctttc atcgcttcat tcaggtcagt gttgagcccg
     9121 atgggtatcc agtatttcga gttttctgcg aacggatttt tcatcggcgc cttcatcggc
     9181 agcgacgcat cgcccggttt cagcacggtc agccgaaacg ttgagcgcaa cgacccttcc
     9241 accgcagtaa gcgccacttc gccatcgccc tgcacgaagt gcgggtcggc ggcgtagaac
     9301 agcgcgccgt caacctgaat cggaatgtag agcgtcgcgc cggttgtgag ttcattcagg
     9361 tccatgttcc cggcatattc gcctggggga accgaatttg gcttgtcttt agtgttaacc
     9421 gcaacaccga aaaggccgtt aaacggctgc agcgggaaac gcgctaccac gccgttcttg
     9481 tctttgatta tggcgtacca tttgccgctt atctgccgga ttggcacgaa aaccgataca
     9541 ttgtgataaa ggtttggatg cgctgcatct gcgcccgcct caggcccctt attttccggg
     9601 aactcgcctg gcagcgcgcc cttgccgtgg cgattggata cgacgccgta aggaacgcgg
     9661 aactgttgcg acaggatctc aaccttgagc acgtcgccgg ctttcgcgcc ttgaatttcg
     9721 accggtccgg tcactatatg cggaccgtcc ttggcgaaat cgtgttcgag ctttgacgct
     9781 gcgattaccc gtgcgtcctt caacacctgt gcaggtttga tgccgaactg gccgaaatat
     9841 ctgaccgggt ccttgccttg atcttcgagg attccttcgt gtgaaacagt gtcgaacgtg
     9901 accacgcttc ccgacggcac cgagagcact gcttttgcct cggcatttgg caggcttccc
     9961 caccgtactg tttcaggagt tgacgggacg taatatccac cccacggcag gacgcccttt
    10021 ttcaccgcca taggcttgtc gccaagaggt tgcaggacct tgaaatccgc ggcctgtgcg
    10081 agcgtgaccg cgcaggcgaa cgtcgccaca gcaagcattg acgcctgctt aagtgcgaat
    10141 gagaccgatg atatagtcat ttcgggagcc tccttgagaa tgattcgatg gcgcacgcgg
    10201 aagcggcgcg aaaaaattag catagcacaa caagaaagat gacgctcacc tgaggcgtga
    10261 ggcatttacc ccagaaaaat cacagcttcc gaggggctga tcaagggtct ggactggcgt
    10321 ccccaaccgg attcgaaccg gtgttaccgc cgtgaaaggg cgatgtccta ggcctctaga
    10381 cgatggggac tcaaggaaaa catttgaaac tggtggaggt aagcgggatc gaaccgctga
    10441 cctcttgcat gccatgcaag cgctctccca gctgagctat acccccacaa aggctgcgaa
    10501 ttatacttga tgctgcgcgc cctaggcaag aaaaccgctt tttattgcgc atggtcggcg
    10561 agctgctcgc gcagtcgttt gagcgcccgt tcgcggccta tcagttccag cgtggcgtcg
    10621 atgggcgggg tctgcgcgct gccggccacc agaacccgaa gcggcatggc cagcaaggga
    10681 agtttcagtc catgcttcac caccacggat ttgatcgctt cattgatagc caaacgctgc
    10741 caatcaattg tcccgaaccg ttccgccact tcacgcagcg cgggaatgac ctcgccattc
    10801 acatgttcta ctataagttc cttgctcggc gggatgtagc ggtaaaacag catggccact
    10861 ccggcgagtt catttagcgt attggcacgg tccttgagca atcgcacgac ttcgacaacc
    10921 tttgggccgg ctgcgatatt tccgccgcgg cgcttgatgt ccggcacaat ctgttcggcc
    10981 agcatcgcgt cttggcctga tttgatgtat tgatgattga gccacaacag cttttccgga
    11041 ttgaactgcg ccggcgactt gctgatgtgc ttcaggtcaa accagctgac aaactgctcg
    11101 attgaaaaca tttccgcgtc gccgtgcgac cagcccaggc gcgccagata attaataagc
    11161 gcttccggca aataaccctc ttccttgtat tgcatcacgc tcaccgcgcc gtggcgtttg
    11221 gagaaccgct cgccgtccgt gcccagtatc atcggcacat gcgcgtatcg cggcaaaggc
    11281 gtccccagcg ctttgaggat gttgatttgc cgcggcgtgt tattgacgtg atcatcgccg
    11341 cgaatcacat gggtaatgcc catgtcgaga tcatccacca ccacgccgaa gttataagtc
    11401 ggcacgccgt ccgcgcgcag aatcaccacg tcgtcgagct cgctgttctg cacgacgatg
    11461 cgccctttca ccagatcatc gaactccacc tcgccatcga gcgggttctt gaagcgcacc
    11521 accggcgtga cacccgcggg aggcatttgt ttcgaatcgc gccaacggcc gtcatagcgc
    11581 ggcttttctc ccctcgcccg ctgcgctttg cgcatcactt ccagttcttc cctgctagcg
    11641 tagcagtagt aggccttgtc ttcctttaac aattgctgcg ctacttcctg atagcgcggc
    11701 agacgctgca tctgataaaa cggcccttcg tcccagttga gcttgagcca gctcatgccg
    11761 tcgagaatcg cctgaatcga ttcccgggtg gagcgctcgc ggtcggtatc ctcgatgcgc
    11821 agc
//
LOCUS       DATIOW010000138         5802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_674875, whole genome shotgun sequence.
ACCESSION   DATIOW010000138 DATIOW010000000
VERSION     DATIOW010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674875"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VLL03_04555"
     CDS             <1..233
                     /locus_tag="VLL03_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HSS46668.1"
                     /translation="GSWIIAFQTGQPGGDVTNHLKGEYISVYVPTTPNPTSGFFLMMP
                     KSEVIELDMSVDDALKYIISMGVAAPGDRIQK"
     gene            331..2115
                     /gene="aspS"
                     /locus_tag="VLL03_04560"
     CDS             331..2115
                     /gene="aspS"
                     /locus_tag="VLL03_04560"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HSS46669.1"
                     /translation="MRTEYCGLVSRKHLQQSVTLCGWVHRRRDHGGVIFIDLRDREGL
                     VQVVCDPDRKQTFQIAEKIRNEFVLKVTGKVRHRPEGTVNPDLVSGEVEVLAHEIEIL
                     NPSLTPPFQLDDENLSETTRLTHRVIDLRRPQMQANLKLRYKVAMAVRKFLDAHGFID
                     IETPMLTKSTPEGARDYLVPSRVYPGQFFALPQSPQLFKQLLMIAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDIETSFLNEGEITALMEELIRNLFKEVMQVELPKPFPRLTYQE
                     AMLRYGTDKPDLRVKLELTELTDLMKAVEFKVFRTATELPDGRVAALRVPKGGELTRK
                     EIDDYTAFVAIYGAKGLAYIKVNEKAKGTEGLQSPILKFLPENVIKAILERTSAQDGD
                     LIFFGADQAKIVNDALDALRMKIGHEKGHAGQGWKPLWVTDFPMFEWNEEEKRWDALH
                     HPFTSPRNGHEAFLDKEPQKALAKAYDMVLNGWEIGGGSVRIHREEVQSKVFRALNIG
                     EEEARQKFGFLLDALQYGAPPHGGIAFGLDRIVALMAGAESIRDVIAFPKTQRAQCLL
                     TQAPSPVDEKQLRELHIRLRDIVKPAAD"
     gene            2191..2583
                     /locus_tag="VLL03_04565"
     CDS             2191..2583
                     /locus_tag="VLL03_04565"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HSS46670.1"
                     /translation="MKNLLFVCVENSCRSQIAEAFARIHAKGWVAIYSAGSRPSGRIN
                     AKAAESMREIGYDLTQHNSKALTEIPDVQYDFVVTMGCGDECPLVRAKQREDCGIPDP
                     KNLPLEQFREVRDLIESKVKDVLVRIET"
     gene            2637..3086
                     /gene="nudB"
                     /locus_tag="VLL03_04570"
     CDS             2637..3086
                     /gene="nudB"
                     /locus_tag="VLL03_04570"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HSS46671.1"
                     /translation="MAKIYKIPVSVLVVIYTPDLQVLLLERADHPGYWQSVTGSLGKG
                     ETPEQAAIREVKEETELDATRYPLADWEIENEFEIFAEWRHRYPPGVTQNTEHVFGLA
                     LPKPLRVTVAPREHLGFAWMPYQEAAQKCFSWSNRDAILTLPRFILH"
     gene            3196..4293
                     /gene="nadA"
                     /locus_tag="VLL03_04575"
     CDS             3196..4293
                     /gene="nadA"
                     /locus_tag="VLL03_04575"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HSS46672.1"
                     /translation="MQVSAIAFDRFGRMQDEACEERILAAKAALGKRAVILGHHYQRA
                     DVYRHADLTGDSLKLSRLAAETSAEYIVFCGVHFMAEVADIVTDQERTVILPDMNAGC
                     SMADMANLAKVERVWRELAEVFDPEQTVTPVTYINSAADLKAFCGEHGGIVCTSSNAG
                     KILEWGFKQREKALFFPDQHLGRWSGHKMGIPLEEMPVWDPDEPMGGLSAEQIRKARI
                     LLWKGHCSVHQMFQPQHVLKFRNQHPDGIVISHPECNFEVCKLSDYVGSTETILKTVK
                     AGKPNTRWLVGTELNLVSRLAEEVKHEGKIVQFMAPTVCMCSTMARIDPQHLAWSLEN
                     LTAGEVVNQIRVPKRDAELARLALNRMLDIS"
     gene            4411..4830
                     /locus_tag="VLL03_04580"
     CDS             4411..4830
                     /locus_tag="VLL03_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol oxidase"
                     /protein_id="HSS46673.1"
                     /translation="MQRFLSGFICCITVVSSGLIFADTQNNADIYTAIVDKDGVQHVR
                     ILGGSYFFKPNRIVVKKNVPVVLSISVESGIAPHSIVAKSPEAGIAFDESLSSTPKNI
                     TFTATAAGEYVFYCKNKLPFLPSHREKGMHGVIEVVE"
     gene            4827..>5802
                     /locus_tag="VLL03_04585"
     CDS             4827..>5802
                     /locus_tag="VLL03_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stilbene synthase"
                     /protein_id="HSS46674.1"
                     /translation="MMFIVGLGTATPPQRYTQSQCWEALQTSEQFKQLDRRSRAILQK
                     VLLGDNGIRTRFLVLNPLTEVFDSTPDKLHQRFTQHAPVLAAQAAECALQQAGNQPGQ
                     MDAVIISTCTGYLCPGLTSYVAERLRLRPDVVALDLVGQGCGAALPNMRTAEALLAAK
                     RCRRVLSICVEICSAAFYLDNDPGVLISACLFGDGAAAAVLSDEPNSHGRRIEWKASA
                     SLTSPLERDALRFEQKNGMLRNILTPPVPRIAARHAEKMLDEMLNKLSISRGQISAWI
                     WHAGGRDVLAALSERLGLTTTDLRWSSKVLSEFGNLSSPFVYFVLQAAL"
BASE COUNT         1318 a         1602 c         1670 g         1212 t
ORIGIN      
        1 aaggctcgtg gatcatcgct tttcaaaccg ggcagccggg tggcgatgtt accaatcatc
       61 tcaaaggcga gtacatcagt gtctacgtgc cgaccacgcc caaccctacg tcaggttttt
      121 ttctgatgat gccgaagagc gaggttatcg agctcgacat gagcgtggac gacgcgctca
      181 agtacatcat ctccatgggt gtcgcggcgc ctggggacag gattcagaag taaggaatca
      241 ggagccagta gcccaccaac caataacaga tgcttggcac tcatgccggc ccaaagaatt
      301 taattttaaa tcccgaatcc tgctttaatc atgcgtactg agtattgcgg cctcgtcagc
      361 cggaagcatt tgcagcaaag cgtgacactg tgcggctggg tgcaccggcg gcgcgaccac
      421 ggcggggtga tcttcatcga cctgcgcgac cgcgaagggc tggtgcaggt agtgtgtgac
      481 cccgaccgca agcaaacttt tcagattgcc gaaaaaatcc gcaatgaatt cgttttgaaa
      541 gttaccggca aggtgcgcca ccgccccgag ggcacggtga acccggactt ggtcagcggc
      601 gaagtggaag tgctggcgca cgagattgaa atcctcaacc cttcgctcac accgccgttc
      661 cagctcgacg acgaaaatct ttccgaaacc acccgcctta ctcaccgcgt gattgacctg
      721 cgccgcccgc aaatgcaggc aaatctcaaa ctgcgctaca aggtggcaat ggcggtgcgc
      781 aaattcctcg acgcgcacgg tttcatcgac attgagacgc cgatgctcac caaatccacg
      841 cctgaaggcg cgcgcgatta cctggtgccg tcgcgcgtct atcccggcca gttcttcgcg
      901 ctgccacagt cgccgcagct gttcaagcag cttttgatga ttgccggctt cgaccgctac
      961 taccagatca ccaaatgctt ccgcgacgag gatctacgcg ccgaccgcca gccggaattc
     1021 acgcagatcg atatcgaaac ctcgttcctg aacgaaggcg aaatcactgc gctgatggaa
     1081 gaactgatcc gtaacctttt caaggaagtg atgcaggtgg aactgcccaa gccatttccg
     1141 cgattgacct atcaggaagc gatgttgcgc tatggcacgg acaagccgga tttgcgcgtg
     1201 aagctggagt tgaccgagct taccgatttg atgaaagcgg tggaattcaa agtgttccgt
     1261 accgcaactg aattacccga cggtcgggtg gcggcgctgc gcgtgcctaa aggcggcgag
     1321 ctgactagaa aagagattga cgactacacc gcatttgttg cgatttacgg cgcgaaaggg
     1381 ctcgcctaca tcaaggtcaa cgaaaaagcc aaaggcacgg aaggtttgca gtcgcccatc
     1441 ctcaagttcc tgcccgagaa tgtcatcaag gcgattctcg agcgcacctc ggcgcaggac
     1501 ggcgatctga ttttctttgg cgcggaccag gcgaaaatcg tgaacgacgc tttagacgcg
     1561 ctgcgcatga aaatcggcca tgaaaaaggc cacgccggac agggctggaa accactgtgg
     1621 gtcacggatt tcccgatgtt cgagtggaac gaagaagaaa agcgctggga cgcgctgcac
     1681 catcccttta cttcgcccag gaacggccac gaggcatttc tcgacaagga accgcagaaa
     1741 gccttggcca aggcctacga catggtgctg aacggctggg aaatcggcgg cggctcggtg
     1801 cggattcacc gcgaagaggt gcaatccaaa gtctttcgcg cgctcaacat cggcgaggaa
     1861 gaagcgcggc agaaattcgg cttcctgctc gatgccctgc aatacggcgc gccgccgcac
     1921 ggcggcatcg cttttggttt ggaccgcatc gttgccctga tggcgggcgc ggaatccatc
     1981 cgcgacgtga ttgctttccc caaaacccag cgcgcgcaat gcctgctcac ccaagcgccc
     2041 agcccggtgg acgaaaagca gttgcgggaa ttacatatcc ggctgcgtga tatcgtcaaa
     2101 cctgcagcgg attgaaaaat agccgacgca aagtaattgc acggcattcc gtgagcatcg
     2161 gagaaggcgg cgggttgcaa aggcacgctc atgaaaaatc ttctgttcgt ttgcgtggaa
     2221 aattcctgcc gcagccaaat tgcggaagct ttcgctcgca ttcatgcaaa agggtgggtg
     2281 gcgatttaca gcgccggttc ccgtccttcg gggcgaataa acgccaaggc ggccgaatca
     2341 atgcgcgaaa ttggttatga cttgactcaa cacaattcaa aggcgcttac ggaaataccc
     2401 gatgtgcaat atgattttgt cgtgaccatg ggctgtggag atgaatgccc gttggtgcgg
     2461 gccaagcagc gggaagattg tggtattccg gaccccaaga atctgccgct tgagcagttt
     2521 cgagaagtgc gcgatttgat cgagagcaaa gtaaaagacg ttctggttag aatcgagact
     2581 tagcgagatt gcgcagcccg gcctgtggtc gagaaaactc acccaatccg tattgagtgg
     2641 caaaaattta caaaatcccc gtatcggtgc tggtggtcat ctacactcct gacttgcaag
     2701 tcttgctgct ggagcgcgcc gaccatccgg gttactggca atcggtgacc ggcagtctgg
     2761 gcaagggcga gacgccggaa caggccgcga tacgcgaagt gaaggaagaa accgaattgg
     2821 acgcgacgcg ttatcccctt gccgattggg aaatcgaaaa cgaatttgaa atctttgccg
     2881 aatggcggca ccgctatccg ccgggggtga cccagaatac cgagcatgtg ttcggcttgg
     2941 ctttgccgaa accgctgcgg gtgacggtcg cgccgcgtga gcatctgggt tttgcctgga
     3001 tgccgtatca ggaagcggcg caaaagtgct tttcctggag caatcgcgac gcgattttga
     3061 cgctgccaag atttatcttg cattaagtaa atacctactg tgatgcaacg ccgggctgga
     3121 atttttgtct tattgtgata atgttagcag tgcaattagc gcaaaggtgg tacctgaact
     3181 ttatgagatt ttcccatgca agtcagtgcg atagcttttg accggttcgg ccggatgcag
     3241 gacgaagcct gcgaggagcg catccttgcg gcgaaggctg ccttgggcaa gcgcgcggtg
     3301 attctagggc accactacca gcgcgccgat gtgtacaggc atgccgatct caccggcgat
     3361 tcgctgaaac tctcacgtct ggcggcggaa acttccgccg aatacatcgt gttctgtggg
     3421 gtgcatttca tggcggaagt ggcggacatc gtcactgatc aagagcgcac cgtgattctg
     3481 ccggacatga acgccggctg ctcgatggct gacatggcga acctcgccaa ggtggagcgg
     3541 gtgtggcgcg agctcgccga agttttcgat cccgagcaaa cggtgacgcc ggtgacctac
     3601 atcaactccg ccgccgattt gaaagcgttc tgcggcgagc atggtggaat cgtctgcacc
     3661 tccagcaacg ccggaaaaat tcttgaatgg ggattcaagc agcgcgaaaa agcgttgttc
     3721 tttcccgacc agcaccttgg gcgctggagc ggccacaaaa tgggcattcc gctcgaggaa
     3781 atgccggtct gggaccccga cgagccgatg ggcgggctta gcgccgaaca aatcaggaaa
     3841 gccagaatcc tgttgtggaa aggccattgc tcagtgcacc agatgttcca accgcagcac
     3901 gtgctcaaat tccgcaatca gcatccggac ggcatcgtga tttcgcatcc tgaatgcaat
     3961 ttcgaggtat gcaagctctc cgattacgtg ggctcgacgg aaaccattct caaaaccgtg
     4021 aaagcgggca aacccaatac gcgctggcta gtgggcaccg agctcaatct ggtgagccgc
     4081 ctcgcggagg aagtaaagca cgaaggcaaa atcgtgcagt tcatggcgcc gacggtgtgc
     4141 atgtgctcga ccatggcgcg catcgatccc cagcacttgg cctggtcgct ggaaaatctg
     4201 acggcgggcg aggtggtgaa tcaaatccgt gtgccaaagc gcgatgcgga gctggcgcgg
     4261 ttggctctga accgcatgct cgatatatct tgagacgttt ttctgttctc gcgtcccctc
     4321 ctttcctaaa atttgttttg aggtgaatct ccggccatgc cgtggtgtca aacccagcct
     4381 gcggttagac tattatcaag gggaaatgcc atgcaacgtt ttttgtccgg cttcatctgt
     4441 tgtattaccg tcgtttccag cgggctgatt ttcgccgata ctcaaaataa tgccgacata
     4501 tacactgcca ttgtggacaa agacggcgtg cagcatgtgc gcatactcgg cggaagctat
     4561 tttttcaaac ccaaccgcat cgtggttaaa aaaaacgtgc cggtggtact ttcgatcagt
     4621 gttgaatcag gtattgcacc ccacagcata gtggccaagt cgccggaagc gggcatcgcc
     4681 ttcgatgaat ccctgagcag cactccaaaa aacatcactt tcactgcgac cgccgcgggg
     4741 gaatacgttt tttattgcaa aaataagctg ccgtttctgc ccagccaccg cgaaaaaggc
     4801 atgcacggcg tgatcgaagt ggtcgaatga tgtttatcgt cggcctgggc accgcaaccc
     4861 cgccgcagcg ctacacgcag tcgcagtgct gggaggcgtt acagacgagc gagcaattca
     4921 agcaacttga ccggcgctcg cgcgccattc tgcaaaaagt tttgctgggc gataacggca
     4981 tccgcacccg gtttctcgta ctcaacccgt tgaccgaggt tttcgattct actcccgata
     5041 aactccatca gcgcttcacc cagcacgcgc cggttcttgc cgctcaggcg gccgaatgtg
     5101 ccctgcaaca agccggtaat caaccggggc aaatggatgc cgtcatcatc agcacctgca
     5161 ccggttattt atgtccgggg ctcaccagct atgtggcgga acgcctccgc ttaaggccgg
     5221 acgtggtggc gctggatttg gtaggacagg gttgtggtgc ggctttaccc aacatgcgta
     5281 ctgcggaagc tttgttggcc gccaaacgct gccggcgtgt gctgtcaatt tgcgtggaaa
     5341 tatgcagtgc ggctttttac ctggataacg atcccggcgt gctcatcagc gcttgcctgt
     5401 tcggcgatgg cgccgccgcc gcggtgctct ccgacgaacc gaattcccac ggccgccgca
     5461 tcgaatggaa agcctccgct tccttgacta gtccgctgga gcgcgatgcg ctgcgcttcg
     5521 aacagaaaaa cggcatgttg cgcaacattc tcacgccgcc ggtgccgcgt atcgccgcaa
     5581 ggcatgccga gaagatgctt gacgagatgc tcaacaaatt gagtatctcg cgcggccaga
     5641 tcagcgcctg gatctggcac gccggcggcc gcgatgtgct cgctgcactc agcgaacggc
     5701 tcggtctcac gacaaccgat ttgcgctgga gcagcaaggt gttgagcgag tttggcaact
     5761 tgagcagtcc gtttgtttat tttgtgttgc aggcggcgct ca
//
LOCUS       DATIOW010000139        16551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_777309, whole genome shotgun sequence.
ACCESSION   DATIOW010000139 DATIOW010000000
VERSION     DATIOW010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16551
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777309"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VLL03_04590"
     CDS             <1..345
                     /locus_tag="VLL03_04590"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HSS46675.1"
                     /translation="LLSPTTTAVRIEQVAKLSRGYVYYVSLKGVTGARHLDLSDVAKK
                     IPQIRARTKLPVGVGFGVRDAQTAKAVSELADAVVVGSRIVEEVENSARQEVLSRVSA
                     LIKELRQAIDQR"
     gene            364..1233
                     /gene="accD"
                     /locus_tag="VLL03_04595"
     CDS             364..1233
                     /gene="accD"
                     /locus_tag="VLL03_04595"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HSS46676.1"
                     /translation="MSWLQKLLPPKIKRNVTGAKKTVPEGLWTKCPMCEAVLYRSELE
                     NNLNVCPKCGHHNRLSARARLDALLDSEGRYEIGAEVASVDTLKFKDDMRYNERLELA
                     QAETDETEALVVMQGSVKTVPLIAAAFEFKFLGGSMGSAVGERFVRGVQVCIDQNLPL
                     VCFTSSGGARMQEGLLSLMQMAKTAAALTQLSEAKLPFISVLTDPTMGGVSASFAMIG
                     DVVMAEPAALIGFAGPRVIEQTVRETLPEGFQRSEFLLEHGAIDMIVDRRQMRDKLVN
                     LITLLMRQPAPTA"
     gene            1254..2543
                     /gene="folC"
                     /locus_tag="VLL03_04600"
     CDS             1254..2543
                     /gene="folC"
                     /locus_tag="VLL03_04600"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HSS46677.1"
                     /translation="MKPELPNPKLQSLSDWLAYLESLNPRSIALGLERVAQVKEALRL
                     QPGFPIITVGGTNGKGSTCAFLESILATAGYRVGCYTSPHLLQYNERVRINRAPVGDA
                     AFLRVFEKIEAARNGIPLTYFEYGTLAAMLLFIEAKLDVAILEVGLGGRLDAVNAFDA
                     DCAAVTGVDFDHMDYLGNTLEQIAFEKAGIFREDRPAICAEPHPPATLIQHARNIGAR
                     LISINRDFGYVAEKNQWQYWGRHGKRHALPYPALRGTYQLQNASAGLAVLDELKQRFP
                     VGMEEIRRGLLETDLLARFQVLPGRPTVILDVAHNPQAAAGLSENLFNMGFYRNTCAV
                     FGMLKDKDIAGMVRRLAPQVDRWFLATIHERRGATADDLARELQQVGIPQNAMQTFDH
                     PGAAYGHACNLAAENDRILVFGSFHTVAGVLRRRSGE"
     gene            2571..3170
                     /locus_tag="VLL03_04605"
     CDS             2571..3170
                     /locus_tag="VLL03_04605"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HSS46678.1"
                     /translation="MARTIGDEQLQLKKRARRRLIGAIALVTLMVLVLPLLLDGESKP
                     VSPDISIQIAPGNGGAFTSRVVPVPEKSGEAVDAEKVQKAENIALPPLFPAAKPNTDK
                     PTESAKTTEKKAGKSAKVESKSGAYVVQLAAFSNSDKAKQLQQKLTANGIRAYTEVLK
                     TAGVEKIRVRAGPYESRDAAEKALERLKTLGMDGVVTSR"
     gene            3176..3667
                     /locus_tag="VLL03_04610"
     CDS             3176..3667
                     /locus_tag="VLL03_04610"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HSS46679.1"
                     /translation="MTVFDFAVLLIIMLSVLLSVIRGAVREVLALAAWVVAFWAAQSY
                     TVEAAALVPGVLSNTSLRLMAGFAVIFLTVLVIMSLVAILCSRFVKATGLSAADRCLG
                     AVFGLARGYLIVLILVLLGGLTSLPKQPLWKDALFSSSLEKVVIYVKTWLPEGLSKRI
                     NYS"
     gene            3680..5206
                     /gene="purF"
                     /locus_tag="VLL03_04615"
     CDS             3680..5206
                     /gene="purF"
                     /locus_tag="VLL03_04615"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HSS46680.1"
                     /translation="MCGILGVVAKTPVNQLLYDGLLVLQHRGQDAAGIVTAEGSTFHM
                     HKGSGMVRDVFRTRNMRELQGNMGIAHVRYPTAGSADSPAEAQPFYVNSPFGIVLGHN
                     GNLTNTEQLKRDLFRQDLRHVNTNSDSEVLLNVLAHELQEACGGHALDAPTIFNAVCG
                     VYRRCRGAYAVGAMIAGYGFLAFRDPHGIRPLVVGQCRSERGIEYLAASESVALDMLG
                     FELMRDVAPGEAIFVDDQGNFYSKQCAAGAMRNPCIFEYVYLARPDSMIDGISVYETR
                     LHMGESLAEKIRRQYHGLEIDVVIPIPDSSRPSAMQLANRLGIPYREGFIKNRYIGRT
                     FIMPGQAIRRKSVRQKLNAMAVEFKGKNVLLVDDSIVRGTTSSEIVQMAREAGARKVY
                     FASAAPPVRFPNVYGIDMPTRQELIANGRNEDEVAREIGADKLIYQDLEALVAAARRG
                     NPQITQFETSCFSGDYITGDVSEEYLAYIEAQRRDGARRQKEDAASSNQLDLNLLTVD
                     "
     regulatory      5229..5307
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            5348..6526
                     /locus_tag="VLL03_04620"
     CDS             5348..6526
                     /locus_tag="VLL03_04620"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HSS46681.1"
                     /translation="MSDEFQLETLALRAGTHRTPFNEHSEALFLTSSFVFDSAAQAAA
                     RFAGDEPGNIYSRFTNPTVTAFQERLSALEGAEACVATSSGMSSILACVMGLLKSGDH
                     IIASHSIFGATVQLFTNIFTRFGLGTTFVSATDVGAWEEAVKPNTKMLFLETPSNPLT
                     EISDIAALSTVAKKAGAWLVVDNCFCTPMLQRPLEFGADIIIHSATKYLEGQGRVLGG
                     AVLGKREVIMEGVFGFLRTAGPTLSAFNAWVLLKRLETLKIRMDAHSANALELARWLD
                     RQPQIERVYYPWLPSHPQHALAKTQQKSGGGIVSFIVKGGKAAAWRVVDATRMISITA
                     NLGDVKSTITHPASTTHGRIPQEARNAAGVVDGLLRIAVGLEALDDIKTDLERGLKSV
                     "
     gene            6528..7097
                     /locus_tag="VLL03_04625"
     CDS             6528..7097
                     /locus_tag="VLL03_04625"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HSS46682.1"
                     /translation="MPRTDNIYQLPDNLPLPVDDGACNHLPGLRLPLIALSATSGGKV
                     DLSSLAGTTVLYCYPRTGRPDQELPPGWNDIPGARGCTPQSCAFRDHHQELRRLSACV
                     FGLSTQTTEYQQEAAQRLHLPFALLSDGELQFVSALRLPTFEVGGMTLTKRLTLIVRD
                     GRIVKVFYPVFPPDQNAEQVINWLSRNPA"
     gene            complement(7132..7854)
                     /locus_tag="VLL03_04630"
     CDS             complement(7132..7854)
                     /locus_tag="VLL03_04630"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF003743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HSS46683.1"
                     /translation="MKPTLFISDLHLCVSRPHINRIFFTFMERIAPKAQALYVLGDLF
                     EYWAGDDDLDDSFNTQVISAFRNFIEQQVPLYIVHGNRDFLMGEEFARACNAKLLPDP
                     SLIDLYGTPTLIMHGDTLCTDDTAYQAFRAQVRDPAWQKGFLSQPLSERKAAIEKLRA
                     QSEQEKSQKSEAIMDVAPATVDKVLREYHYPRLIHGHTHRPAKHVHVVDGKNCERWVL
                     SDWYKHGEYLRCDESGCSAIKL"
     gene            complement(7946..8437)
                     /locus_tag="VLL03_04635"
     CDS             complement(7946..8437)
                     /locus_tag="VLL03_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSS46684.1"
                     /translation="MVKFHTNLGPITLELDAGKAPLTVANFLQYVESGFYGNTLLHRV
                     IDGFMIQGGGFEPGMKQKATRGPIKNEADNGLKNEAYTIAMARTSNPHSASSQFFINV
                     ADNSFLNFNAATPQGHGYCVFGRVVDGADVVDKIRKVKTGMRAGHEDVPVQVVIIERA
                     EVV"
     gene            complement(8484..9716)
                     /locus_tag="VLL03_04640"
     CDS             complement(8484..9716)
                     /locus_tag="VLL03_04640"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HSS46685.1"
                     /translation="MSQLQQRSLTTLFKCTCAAALALTLAGSASSTNEQVLQASLVRP
                     LPTPEKLLVNSIMEISRNNLDAAMQEIDNALKTYPNFRLLHLIKGDLLLARARPLSTI
                     GDAPNAPRQRVAELREEAIARLQRHRLALPLDRLPKYLLQMQPQQQYAVVVDISRSTL
                     YLYQNINGEPRYVSDYYISSGKNGSEKLKEGDQRTPLGVYFVTANLPKAQLSDFYGAG
                     AYPISYPNEWDRRHGRNGHGIWLHGTPSDTYSRPPRSSNGCVVLTNQDLQVLSKNLQI
                     GLTPVIISERVDWIDPEAWRAQRNTLAQEVEAWRRDWESRDTEAYLRHYGRNFFVKSQ
                     NFNQWARQKRQINASKIWIKVGVSNVSMFLYPGYENLAVVNFEQDYSSNNLSAKMKKR
                     QYWIKENNRWKIAYEGAA"
     gene            complement(9713..10711)
                     /locus_tag="VLL03_04645"
     CDS             complement(9713..10711)
                     /locus_tag="VLL03_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS46686.1"
                     /translation="MKKPDIAALLTLLTLLAFGLPAAADDLEDAYALFKQGNHAQAQD
                     KVSSFLSGHPKDAKARFLLGLIYTEQNKSDDAIKIFTGLTEDYPELPEPYNNLAVLYA
                     AQEQYDKARTALEAAIRTHPSYATAHENLGDIYAKLATQAYDKALQLDKNNTTAKTKL
                     ELIKDLFVGSPRTARVAAKTEITKAGSSVAANKSAPQTETSQVAKAAPAVDENKTASK
                     SSDDEVIKTVNDWAKAWSKGDADAYLAFYAQDFKTPNNESREAWENSRKARISKSKAI
                     QVSVIDPKVVLDDATHASVKFKQGYRSSTLKSTSAKTLYLVRSGDKWLIQREQVGR"
     gene            10870..12297
                     /gene="cysS"
                     /locus_tag="VLL03_04650"
     CDS             10870..12297
                     /gene="cysS"
                     /locus_tag="VLL03_04650"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HSS46687.1"
                     /translation="MLKIYNTLAREKQAFKPIYSGRVNMYVCGMTVYDYCHLGHARVL
                     VVFDMVQRWFRASGYAVNYVRNITDIDDKIIKRAVENHEPVDALTARFIQFMEEDAQA
                     LAVEKPVSEPRATEHVGDMVRMIKTLVDKGLAYSADNGDVYYAVHKFPGYGRLSGKSL
                     EDLRAGERVEIGTEKRDPLDFVLWKAAKPDEPWWDSPWGKGRPGWHIECSVMSEKLLG
                     HHFDIHGGGQDLQFPHHENEIAQSEGANGCTFVNYWMHNGFVRVDEQKMSKSLGNFFT
                     VREVLKKYDAEVVRFFILRAHYRSPLNYSDQHLDDARQALTRLYTALKGIDAASVKLD
                     WQEPYAARFKEAMDDDFNTPEATAVLFDLANEVNKTHSPQQAGVLKALGGILGLLERE
                     PRQYLQSAPKATSTIVHAIGGGKVSVTAVKDYPPESIEQLIAARAAARKVKNFAESDR
                     IRKELLEVGIVLEDKPDGKTEWRRI"
     gene            12449..13597
                     /locus_tag="VLL03_04655"
     CDS             12449..13597
                     /locus_tag="VLL03_04655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSS46688.1"
                     /translation="MFYSNPNPSASPIAAALVAAGLILLLPACSGQDSQAKPSKALGP
                     AVPVTAAAVEKKTMPVRIRGIGNVEAYATVSVKARVDGQIVKVHFKEGQEVPKGAPLF
                     EIDSRPFEAQLHQAEANLLRDKAQLENASAQERRYQDLLNKNFVSKEFYAQIRTNRDA
                     AEATARADQAAVENARVQLEYTRIRSPINGHTGKIMIQEGNLVKANDTNSLVVINQIS
                     PIYVNFSVPEQTLSEIRRYQTQGTLNVAVTLPDTATPPIVGTLAFIDNSVDPATGTIK
                     LKALFENQEKLLWPGQFAHVALTLYEQQGALVVPSQAVQTGPKGQYVFVVKPDFTTEM
                     RDVAVAWADSGQTVIAKGLAAGEKVVTSGQLRLTPGAKVELKTTQAGS"
     gene            13594..>16551
                     /locus_tag="VLL03_04660"
     CDS             13594..>16551
                     /locus_tag="VLL03_04660"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSS46689.1"
                     /translation="MNVPELFVKRPVMTILVMMGILLFGLISYRLLPVSALPNVDFPT
                     IQVSAELPGASPETMASAVATPLEKQFSTVPGVDSMSSVSALGVSQITMQFSLDRNID
                     AAAQDVQSAISLAQKQLPPTMSTPPSYRKVNPADAAIFYLALTSDTLPLSTVDEYAET
                     LMAQRISMINGVAQVQVFGAQKYAVRVRLDPNLLAAKGIGIDEVEKALDQHNVNLPTG
                     TLNGQHSTVTIQASGQLQDAAAYRPMIVTYRNGAPVRLHELGQVIDSVQNDKVAAWYN
                     GGRGIVLAIQRQPGTNTVEVVDNIKKLLPDFRAQMPAGVNMAVLYDRSESIRASVNDV
                     QFSLFLALALVVMVIFLFLRNLPATVIPSLALPFSIIGTFSVMYLLGYSLDNLSLMAL
                     TLCVGFVVDDAIVMLENITRHMESGESALQATLNGSKEIAFTILSMTLSLAAVFFPVF
                     FMGGVLGKLLHEFAVTIITAVLISGFVSLTLTPMLCSRLLRPAHQQHGRLYAASEAFF
                     EGMKHIYDISLKWTLRHQRLTLAVFAAIVIATGWLFVIMPKGFLPNEDTGQLFVFTEA
                     AQDVSFEAMAKQQQAVADIVRADPNVASIMSFIGASGSSQSLNLGRIFIRLKPRGQRL
                     SADEVIQELRPKVEAIPGIKVYLQNLPTIRIGGMLSKSQYQYTLQDADTEVLYHWAPL
                     IEAKIRQLPGFRDVTSDLQISNPQVMVEIDRDKASALGVTSQQIENALYDAYGARQVS
                     TIYTPSNEYWVIMELDPKYQADAQALSMLYVRSGGGTLVPLSSVARLSPGVGTLTINH
                     LGQLPAVTISFDLQPGVSLSDALRQIEKVKGELGAPATLNASFQGAAQAFQASLQNMG
                     YLLAMSILVIYLVLGILYESFVHPLTILSGLPTAGLGALITLLLFHYDLDMYAFVGII
                     MLVGIVKKNAIIMIDFAIEAQRAHGKKPFDAIYEACIVRFRPIMMTTMAALLASLPIA
                     LGVGTGSEARRPLG"
BASE COUNT         3357 a         4657 c         5026 g         3511 t
ORIGIN      
        1 ctgctttctc caacaaccac agcagtccgc attgagcaag tagccaaact atcccgcggt
       61 tatgtgtatt atgtttcact gaaaggcgtg accggagcca ggcatctgga tttaagcgat
      121 gtggcaaaaa aaattcctca aatccgcgcg cgcaccaagc tgccagttgg cgtaggcttt
      181 ggcgttcgtg acgcgcaaac ggcaaaagcg gtgtcggaac ttgctgacgc cgtggtggtg
      241 ggaagccgca ttgtggaaga agttgaaaat tccgccaggc aggaagtgtt gagcagggtt
      301 tctgccctga tcaaagagtt gcgccaggcg attgatcaac gctaagcggg cgtaggacga
      361 ctgatgagct ggcttcagaa actgctgccg ccgaaaatca agcgcaacgt aaccggggcc
      421 aagaaaaccg tccccgaggg gttgtggacc aagtgcccga tgtgcgaagc ggtgctctat
      481 cgcagcgagc tggaaaacaa tctgaatgtc tgccccaagt gcggccatca taaccgcctc
      541 tcggcgcgcg cgcgcctgga cgccttgctg gatagcgaag gccgctacga aatcggcgcc
      601 gaagtggcgt cggtggatac cttgaaattc aaagatgaca tgcgctacaa cgaacgcctg
      661 gagctggcac aagccgaaac ggatgaaact gaggcgctgg tggtgatgca gggcagcgtc
      721 aagaccgtgc cgctgatagc cgcggcgttt gaattcaaat ttctcggcgg gtcgatgggt
      781 tcggcggtcg gtgagcgctt tgtgcgtggc gtgcaggtat gcattgatca gaacctgccg
      841 ctggtgtgtt ttacctcaag cggcggtgcg cgcatgcagg agggcctgct gtcgctcatg
      901 caaatggcga agacggccgc ggcgcttacg caactttccg aagcgaaatt gcctttcatt
      961 tcagtgctga ccgatcccac catgggcggc gtgtcggcaa gtttcgccat gatcggcgat
     1021 gtagtaatgg ccgaaccggc ggcgttgatc gggtttgccg gtccgcgggt gatcgagcag
     1081 accgtgcgcg agacgctgcc ggaaggtttt cagcgctctg aattcctgct ggagcacggc
     1141 gcgattgaca tgatcgttga ccgccggcag atgcgcgaca agctcgtcaa cctgattacg
     1201 ctcttgatgc ggcaaccggc accgacggcc tgagtctgcg gcctcccttc attttgaaac
     1261 ctgagctgcc caatcccaag ctgcaaagct taagcgactg gcttgcctat ctggaaagcc
     1321 tgaacccgcg ctcgattgcc ttgggcctcg agcgcgtagc gcaagtcaag gaagcgctca
     1381 ggctccaacc cggatttccc ataattacgg tgggcgggac taacggcaaa ggctcaacct
     1441 gcgcgtttct ggaaagcata ctcgcgactg ccggttatcg cgtcggctgc tacacctcgc
     1501 cgcacctgct ccaatacaac gaacgcgtgc gtatcaaccg ggcgccggtg ggggatgccg
     1561 catttctgcg cgtgttcgag aagattgaag cggcgcgcaa cggcatcccg ctaacttatt
     1621 tcgaatacgg gactctcgct gcgatgctgc ttttcatcga ggcgaagctc gatgtggcga
     1681 tcctggaagt gggtctgggc ggcagacttg atgcggtgaa cgctttcgat gccgattgcg
     1741 cggcggtgac gggcgtggat ttcgaccaca tggattatct cggcaacacg ctggaacaaa
     1801 tcgcctttga aaaggccggt attttccgcg aagaccggcc cgctatttgc gccgagcccc
     1861 atcctcctgc aaccctgatt cagcatgcgc ggaatatcgg cgcgcgcctt atttcgatta
     1921 atagggattt cggctacgtg gcggaaaaaa accagtggca atactgggga cgccacggca
     1981 agcgtcacgc gctgccttat ccggcgctgc gcggcacgta tcaattgcaa aatgccagcg
     2041 ccggtttggc cgtgctggat gagttgaaac agcggtttcc ggtgggcatg gaggaaatcc
     2101 gccgcgggct gctcgaaact gacttgctgg cgcgcttcca agtgctgccg ggacggccca
     2161 cggtgattct cgatgtggcg cacaatccgc aagccgctgc ggggttatcg gagaaccttt
     2221 tcaacatggg tttttaccgc aacacctgcg cggtgttcgg catgctcaag gacaaagata
     2281 tcgccggcat ggtgcggcgg cttgccccgc aggtggaccg ctggtttctt gccactatcc
     2341 acgagcgccg cggcgccacg gcggatgacc tggcgcggga attgcagcag gtgggaatcc
     2401 cgcaaaacgc catgcaaacc tttgaccatc ccggcgcggc gtatgggcac gcctgtaatc
     2461 tggcggccga aaatgataga attctcgttt tcggctcttt ccatactgtt gccggggtgt
     2521 tgcgccggcg cagcggcgag taaaacctta ggaagggaat gtttgcgcct gtggcaagaa
     2581 cgatcggcga cgagcaattg caactcaaga agcgcgcccg gcggcgcctg atcggggcca
     2641 ttgcgctggt aacattgatg gtgttggtgc tgccgctgct cctcgatggc gagtccaagc
     2701 cggtaagccc cgacatcagc atccagattg cgcccgggaa cggcggcgct ttcacttcac
     2761 gcgtggttcc ggttcccgag aaatcgggtg aagcggtgga cgcagagaaa gtgcaaaaag
     2821 ccgagaacat tgcgcttcca cccctattcc ccgccgccaa accgaatacc gataaaccca
     2881 ccgaatccgc taaaaccacc gagaaaaaag ccgggaaatc cgcgaaggta gagagcaaat
     2941 ccggcgctta tgtggtacag cttgctgcgt tttccaatag cgacaaggca aagcagttgc
     3001 agcagaagct gactgcaaac ggcatccgcg cctacaccga agtgctgaaa acagcgggag
     3061 tagaaaaaat ccgggtacgt gccggtcctt atgaaagccg cgatgccgcg gaaaaagcgc
     3121 tggagcgcct gaaaacgctg ggcatggacg gagtggtgac ttccaggtag cgggcatgac
     3181 agtttttgat ttcgcagtct tgctgattat tatgttgtct gttttgttga gcgtgattcg
     3241 cggcgcggtg cgtgaggtgc tggcgctggc ggcctgggtg gtggcgttct gggcggcgca
     3301 atcgtatacg gtggaagcgg cggcgctggt gccgggtgtg ttaagcaata cgtcgctgcg
     3361 gctgatggcg ggatttgcgg tgatatttct gacagtgctg gtgatcatga gcctggtcgc
     3421 gattctatgc tccagattcg tgaaagccac gggtttgagc gcggccgacc gctgcttggg
     3481 cgcggtattc ggcctggcgc gaggttactt gatagtgctg atattggtgc tgctgggagg
     3541 ccttacatcg ctgcccaagc agccgttgtg gaaggacgcg ttattcagct catcgctgga
     3601 aaaagtggtc atctacgtta aaacctggct gccagaaggc ctgtcaaaac ggattaacta
     3661 ctcatagaag gaaacgggaa tgtgcggaat tctcggcgtc gtggcgaaga ccccggtcaa
     3721 tcagctcctg tatgatggac tgctggtgct gcagcaccgc ggccaggatg ccgccggcat
     3781 cgtcaccgcc gaaggcagta ccttccacat gcacaaaggc agcggcatgg tgcgcgacgt
     3841 gttccgcacc cgcaacatgc gcgagctgca gggcaatatg ggcatcgccc atgtgcgcta
     3901 tcccaccgcc ggttccgccg actcgcccgc cgaagcgcaa ccgttctatg tcaactcgcc
     3961 tttcggcatt gtgctcggcc acaacggcaa tctcaccaac accgaacagt tgaagcgcga
     4021 tttgttccgt caggacctgc gccacgtcaa caccaattcc gattcagagg ttttgctgaa
     4081 tgttctcgcg cacgagctgc aggaggcctg cggcggccac gcgctggacg cgccgaccat
     4141 tttcaacgcg gtgtgcggtg tctaccgccg ctgtcgcggc gcctacgccg tgggggcgat
     4201 gattgcgggc tacggcttcc tcgcgttccg cgatccgcac ggcatccgcc cgctggtggt
     4261 ggggcagtgc cggagtgagc gcggcatcga gtacctggcg gcctcggaga gcgtggcgct
     4321 ggatatgctg ggattcgagc tgatgcgcga cgtggcgccc ggcgaggcga ttttcgtcga
     4381 cgatcagggc aatttctaca gcaagcagtg cgccgccggc gccatgcgca atccgtgcat
     4441 tttcgagtac gtgtatctgg cgcggccgga ctcgatgata gacggcattt ccgtatacga
     4501 aacgcgtctg cacatgggcg agagtcttgc cgaaaagatt cgccggcaat atcacggcct
     4561 tgaaatcgac gtggtgatcc cgatccccga ttccagccgc ccgagcgcga tgcaactggc
     4621 gaaccgtctc ggcattccct atcgcgaagg cttcatcaag aaccgctaca tcggccgcac
     4681 tttcatcatg ccggggcagg cgatacgcag aaaatcggtg cggcagaagc tcaacgcgat
     4741 ggcggtggaa ttcaagggca agaacgtgct gctggtggat gattccatcg tgcgcggcac
     4801 caccagcagc gagatcgtgc aaatggcgcg cgaagccggg gcgcgcaagg tgtatttcgc
     4861 ctcggctgcg ccaccggtgc gcttccctaa cgtctacggc atcgacatgc cgacccgcca
     4921 ggaactgatt gccaatggcc ggaatgagga cgaagtggcg cgcgagatcg gcgccgacaa
     4981 gctgatttac caggatttgg aggcgctggt ggccgcggcg cgcaggggca acccgcagat
     5041 tacgcagttc gagacttcct gcttcagcgg cgattacatc accggcgatg tgtccgagga
     5101 atacctcgct tacatcgaag cgcagcggcg cgacggcgcg cgcaggcaaa aggaggacgc
     5161 cgcttccagc aaccagcttg atttgaattt gcttactgtg gattgacaat tcgattttga
     5221 cagagtaagt tagagactgc gccgatttga tatcagcttg cgggcgcgtt ataaatgccg
     5281 ctaaagcgag gccaaacccg gtccgcccaa agcgaaacgg gttttttgtt ttttaaaggg
     5341 cggaatcatg tctgacgaat ttcaattgga aactttggcg ctgcgcgcgg gcacgcaccg
     5401 cacgccgttc aacgagcatt cggaggcgct gtttctgact tccagcttcg tgttcgacag
     5461 cgcggcgcaa gcggcggcgc gctttgccgg cgacgagccg ggcaatattt attcgcgctt
     5521 caccaatccc accgttacgg ctttccagga gcggcttagc gcgctggaag gcgcggaagc
     5581 ctgtgtcgcc acgtcgtccg gcatgtcctc cattcttgct tgcgtcatgg ggttgctcaa
     5641 gagcggcgac cacatcatcg cgtcgcacag cattttcggc gccacggtac agctcttcac
     5701 caatattttt acgcgcttcg gactgggaac cacttttgtt tccgcgaccg atgtcggcgc
     5761 ttgggaagaa gcggtaaaac cgaacacgaa aatgctgttc ctggaaacgc cgtcgaaccc
     5821 gctgaccgaa atttctgaca tcgccgcgtt gagcacagta gcgaaaaaag ccggagcatg
     5881 gctggtggtg gacaactgtt tttgcacgcc gatgctgcag cggccgctgg aattcggcgc
     5941 cgacatcatc attcattccg cgaccaagta tctcgaagga cagggtcggg tgctgggcgg
     6001 cgcggtgctg ggcaagcgcg aagtgattat ggaaggcgtg tttggttttc tgcgcacggc
     6061 ggggcccacg ttaagcgcgt tcaacgcctg ggtgttgctc aagcgtttgg agacgctgaa
     6121 aatccgcatg gatgcgcatt cggcgaacgc cttggagctt gcgcgctggc tggacaggca
     6181 gccgcaaatc gagcgcgtgt attatccgtg gttgccttcg catccgcagc atgcgcttgc
     6241 gaaaacacag cagaagtccg gcggcggcat cgtgtcgttc atcgtcaaag gcgggaaggc
     6301 agcggcgtgg cgggtggtgg acgccacgcg catgatttcg atcaccgcca atctcggcga
     6361 tgtgaaaagc acgattaccc atcccgcatc cacgacacac ggacgcattc ctcaggaagc
     6421 gcgcaacgcg gccggcgtgg tggatggctt actgcggatt gcggtgggct tggaagcgtt
     6481 agacgatatt aagaccgacc ttgagcgcgg tttgaaaagc gtttagcgtg ccaagaaccg
     6541 acaacattta ccagctgcct gataatctgc cactcccggt ggatgacggc gcgtgtaatc
     6601 atctgcctgg tttgcgcctg cctttgatag ccttgtccgc aaccagcggc ggcaaggtgg
     6661 acctatcgag cctggccgga accacagtgc tttactgtta tccccgcacc ggcaggccgg
     6721 atcaggaatt gccgccgggc tggaacgata ttcccggagc gagaggctgc acgccgcaga
     6781 gctgcgcctt tcgcgaccat caccaggaac tccggcgact cagcgcgtgc gtttttggct
     6841 taagcacgca aaccaccgaa taccagcagg aagccgcgca aaggctgcat ctaccctttg
     6901 cgttattaag cgacggggag cttcaattcg tcagtgcgct gcgtctgccc acttttgaag
     6961 tgggagggat gacactgacc aagcggctta ccttgatcgt gcgcgacggg cggattgtga
     7021 aagtgtttta tccggtgttt ccgcccgatc agaacgcgga gcaagtcata aactggcttt
     7081 cgcgcaaccc ggcgtagccg ctattagcct gtctttcgtg aatagttgag gttacagctt
     7141 gatggcgctg cagccggatt cgtcgcagcg caggtattcg ccgtgcttgt accaatcgga
     7201 caacacccag cgctcgcagt tcttgccgtc caccacatgc acgtgtttcg ccgggcgatg
     7261 ggtgtggccg tgaatcagcc gcggataatg gtattcgcgc aggactttgt ccactgtcgc
     7321 cggagccaca tccatgatgg cttcggattt ctggctcttc tcctgttcgc tttgcgcgcg
     7381 cagtttttca attgccgctt tgcgctcgga cagaggctgc gagaggaagc ctttctgcca
     7441 cgccgggtct cgcacctggg cgcggaacgc ctggtaggcg gtatcatcgg tgcacagggt
     7501 gtcgccgtgc atgatgagcg tcggcgtgcc gtagaggtca atcaggctgg ggtcgggtag
     7561 aagttttgcg ttgcaggctc gggcaaactc ttcacccatg agaaaatcac ggttgccgtg
     7621 aacgatgtaa agcggcacct gctgctcgat gaaattgcgg aaggcggaaa tcacctgcgt
     7681 attgaaagaa tcatccaaat cgtcgtcgcc ggcccaatat tcgaacaaat cgcccagcac
     7741 atagagcgcc tgtgctttcg gcgcgatgcg ttccatgaaa gtgaagaaaa tacggttgat
     7801 gtgcgggcgg ctcacgcaca ggtgcaaatc ggaaatgaac agggtgggtt tcataaggaa
     7861 tcaggattcg ggataaacac gcatggcagg gttccacgag tagaatttgg cccggatctt
     7921 ggctcctgaa tccttaatcc tgcttttaga caacttcggc gcgctcgatg atgacaacct
     7981 gcaccggcac atcctcatgc ccagcgcgca tcccggtttt gaccttcctg attttgtcga
     8041 ccacgtccgc gccgtccacc acgcgcccga acacacagta gccgtggcct tgtggcgtcg
     8101 ccgcgttgaa gttaaggaag gagttgtcgg cgacattgat gaaaaactgg ctgctggcgg
     8161 aatgcggatt cgaggtacgc gccatggcga tggtgtaggc ctcgtttttg aggccgttat
     8221 cggcctcgtt cttgattggc ccgcgcgttg ctttctgctt cattcccggc tcgaagccgc
     8281 caccctgaat catgaagccg tcgatgacgc ggtggagcag ggtgttgccg taaaaaccgc
     8341 tttccacata ctggaggaaa ttggccacgg taagcggcgc tttcccggca tcgagttcaa
     8401 gggtgatggg gccgaggttg gtgtggaact tgaccatttt tcatcctttg ccggcggcag
     8461 cgggaaggaa accggaaact ggtttacgcc gcgccttcgt aagcaatctt ccagcggttg
     8521 ttctccttga tccagtattg ccgttttttc atcttggcgg agagattgtt gctggagtaa
     8581 tcctgctcga agttgaccac cgcgaggttt tcataaccgg ggtagaggaa catgctgacg
     8641 ttggatacgc cgaccttgat ccagattttc gaggcattta tctgccgctt ttgcctggcc
     8701 cattggttga aattctggct cttgacgaaa aaattcctgc catagtggcg caaatacgcc
     8761 tcggtatcgc ggctttccca atcgcgccgc caggcttcaa cttcctgcgc cagggtattg
     8821 cgctgcgcac gccaggcttc cggatcaatc caatccacac gctcgctgat gatgaccgga
     8881 gtcagcccga tttgcaggtt cttggagagt acctgcaggt cctggttggt gagcaccacg
     8941 cagccattgc tggagcgcgg cggtcggctg taggtgtcgc ttggtgtgcc gtgcagccag
     9001 atgccatggc cgttacggcc gtgccggcgg tcccactcgt tcgggtagct gatggggtag
     9061 gcacccgcgc cgtagaaatc ggaaagctgc gctttcggca gattagcggt gacgaaatac
     9121 accccgagcg gcgtgcgctg atcgccttcc ttcaattttt ctgagccgtt cttgccgctg
     9181 ctgatgtagt aatcggagac gtagcgcggc tcgccgttga tgttttgata caggtacagc
     9241 gtggaccgcg aaatgtccac caccacggcg tactgctgct gcggctgcat ctgcagcagg
     9301 tatttgggca ggcggtcgag cggcagcgca aggcggtggc gctgcaggcg ggcgatcgct
     9361 tcttcgcgca attcggccac gcgctgccgt ggcgcgttcg gcgcatcacc gatggtgctc
     9421 aagggacgcg cacgcgccag cagcaaatcg cccttgatta aatgcaaaag ccggaagttg
     9481 ggataggtct tgagcgcgtt gtcgatttcc tgcatcgccg cgtcgagatt attgcggctg
     9541 atttccatga tgctgttgac cagcagcttt tccggcgtcg gcagggggcg caccaaagaa
     9601 gcctgcagga cctgctcatt cgtcgaggac gcgctgcccg ccaaagtcaa agccagcgcc
     9661 gcggcgcagg tgcatttgaa tagcgtcgtc agggaccgtt gttgcagttg gctcaacggc
     9721 ctacctgttc cctttggatc aaccatttgt cccccgatct tacgaggtac agggttttgg
     9781 cgctggtgct ttttagggtg ctggagcggt aaccctgctt gaacttcacg ctggcgtgag
     9841 tggcgtcatc gagcacaact ttgggatcga tgaccgaaac ctgtatcgct ttggatttgg
     9901 aaatgcgcgc tttgcgcgag ttttcccagg cttcgcggct ctcattgttg ggcgtcttga
     9961 agtcctgggc atagaaagcg agataggcgt ccgcatcgcc cttcgaccag gccttggccc
    10021 agtcgtttac cgtctttatc acctcgtcat cactgctttt gctcgccgtt ttgttctcgt
    10081 cgacagcggg agcggctttc gccacttgcg aagtttcggt ctgtggcgcg gatttgttcg
    10141 ccgccacgct tgagccagct ttggtgatct cggttttcgc cgcgaccctg gccgtgcgcg
    10201 ggctgccgac gaagaggtcc ttgataagtt ccagtttggt cttcgcggtg gtgttgttct
    10261 tgtcgagttg caatgccttg tcatacgcct gggtagcgag tttggcgtaa atgtcgccca
    10321 ggttttcgtg cgcggtggcg tagctcggat gagtgcgtat cgccgcttcc agcgcggttc
    10381 gtgccttgtc gtactgttcc tgcgctgcat acagcacggc caggttgttg tagggttcgg
    10441 gcagttccgg gtaatcttcg gtaaggccgg tgaagatctt gatggcgtca tctgacttgt
    10501 tctgctcggt gtaaatcaag cccagcagaa aacgcgcctt ggcgtccttg ggatggccgg
    10561 aaagaaagct gctgaccttg tcctgcgcct gggcatggtt gccctgcttg aacaaggcat
    10621 aggcgtcctc caggtcgtcg gctgccgcgg gcaggccgaa tgcgagaaga gtcagcagcg
    10681 tcagtaaagc ggcgatatcg ggttttttca ttgtggtata ctttttcgcc tttaacaaaa
    10741 tatacagatt ctaccaagaa gcccgctcaa tccacaatca cccatgatac atgcagttcg
    10801 ctaatcaacc ctgtttttcg ccgttctgat tcgggaatac ctccccgacc gccgcattcc
    10861 acgcaaccca tgctgaaaat ctataacact ttggcccgcg aaaagcaggc tttcaagccg
    10921 atttactccg gcagggttaa catgtacgtc tgcggcatga cggtgtacga ctattgccac
    10981 ctcggccacg cccgcgtgct ggtggtattc gacatggtgc agcgctggtt cagggcatcg
    11041 gggtatgcgg tgaattatgt gcgcaacatt accgacatcg acgacaaaat catcaagcgc
    11101 gccgtggaaa accatgagcc ggtggacgcg ctgacggcgc gtttcattca attcatggag
    11161 gaagacgcgc aagcgctggc cgtggaaaaa ccggtatccg agccgcgcgc cacagaacat
    11221 gtcggcgata tggtgcggat gataaagacg ctggtggaca agggactggc ctattcggcg
    11281 gacaacggcg acgtgtatta tgccgtgcac aagtttcccg gttacggcag gctctccggc
    11341 aaatcgctcg aggatttgcg cgccggcgag cgggtggaaa tcggcacgga aaagcgtgac
    11401 ccgctggact tcgtgctgtg gaaggcggcc aagcccgatg agccgtggtg ggattcgccg
    11461 tgggggaagg gccgccccgg ctggcatatc gagtgctcgg tgatgagcga gaaactgctg
    11521 ggtcatcact tcgatattca cggcggcggc caggatttgc agttccccca tcacgagaac
    11581 gagattgcgc aatccgaggg cgccaacggc tgcactttcg tcaattactg gatgcacaac
    11641 ggcttcgtgc gcgtggacga gcaaaagatg tccaagtcac tcggtaattt cttcaccgtg
    11701 cgcgaagtgc tgaagaaata cgacgccgaa gtggtgcgct tcttcatcct gcgcgcgcat
    11761 taccgcagtc ccttgaatta ttccgaccag cacctcgacg atgcgcggca ggcgctgacg
    11821 cggctctaca ccgcgttaaa aggcattgat gccgcttccg tcaaactcga ctggcaagag
    11881 ccttatgccg cgcgcttcaa ggaagcgatg gatgatgact tcaacactcc cgaagccacg
    11941 gcggtcctgt ttgaccttgc gaatgaagtg aacaagacac attcaccgca gcaggcggga
    12001 gtactgaaag ccctgggtgg aattctggga ctgctagagc gtgaaccccg gcagtacctt
    12061 cagtcagctc caaaggcgac ttctacaata gtccatgcaa ttggtggagg taaggtttcg
    12121 gtaactgctg tcaaggacta tccgcctgaa agcatcgaac agctgattgc ggcgcgagca
    12181 gctgcgcgta aagtaaaaaa ttttgccgaa tccgaccgca tccgcaagga gcttttagaa
    12241 gtagggattg ttctcgaaga caaacccgac ggcaagacag agtggcggcg gatttagccg
    12301 caaacaattt ccttcgccct taggcttttt aatccctccg ggcaacaccg gcgctgccgt
    12361 ttggtgtaga ttatggtaag ttaccggccg cgaactttat ccctcgaatc ttttccaatt
    12421 cttgaccgca cacgaagaga agtaacactt gttttattcc aacccgaatc caagcgcatc
    12481 gccgattgcc gccgccctgg tggcggcggg cctgatcctc cttctcccgg cctgtagcgg
    12541 gcaggactcc caggccaaac ccagcaaagc gctcgggccg gcggtgccgg tgaccgccgc
    12601 cgctgtggaa aagaaaacca tgccggtgcg cattcgcggc atcggcaatg tggaagccta
    12661 cgctactgta tcagtgaagg cgcgagtgga cggccagatc gtcaaggtgc atttcaagga
    12721 agggcaggag gtgccgaagg gcgcgccgct gtttgaaatc gactcgcgcc ccttcgaagc
    12781 gcagctgcat caggccgaag ctaatttgct gcgtgacaag gcgcagctgg agaacgccag
    12841 cgcgcaggag aggcgctacc aggatttgct gaacaagaat ttcgtttcga aggaattcta
    12901 tgcgcagatc cgcaccaatc gcgacgccgc tgaagccacc gcccgcgccg accaggcagc
    12961 agtggaaaat gccagagtgc agttggaata caccaggatc cgctcgccga tcaatggcca
    13021 caccggaaag atcatgatcc aggaaggcaa tctggtgaaa gcgaacgaca ccaattcact
    13081 ggtggtgatc aatcaaatca gcccgattta cgtgaatttc tccgtgcccg agcaaaccct
    13141 aagtgaaatc cgccgctacc agacgcaagg gacgttgaac gttgccgtga ctttgcccga
    13201 cactgctact ccgccgatcg tgggcacgct tgcgtttatc gacaacagcg tggatccggc
    13261 gaccggcacg atcaagctca aggcgctgtt cgagaaccag gaaaagttgc tgtggcccgg
    13321 ccagtttgcc catgtcgctc tcacgttgta tgagcagcag ggcgcgctgg tggtgccaag
    13381 ccaggcggtg caaaccggtc ccaagggcca atatgtgttt gtcgtcaagc ccgatttcac
    13441 caccgaaatg cgcgatgtgg cagtggcttg ggcggacagc ggacaaaccg tgattgccaa
    13501 aggcttggcg gcgggggaga aagtggtgac cagcggccag ttgcgtctca cgcccggtgc
    13561 caaggttgaa ctcaaaacca cgcaggccgg ttcatgaatg tccccgagct tttcgtgaag
    13621 cgtccggtca tgaccattct ggtcatgatg gggatcttgc tgtttggcct catcagctac
    13681 cgcctgttgc cggtgagtgc gctgcccaat gtagatttcc ccaccattca agtgtcggcg
    13741 gagcttccgg gagcgagccc cgaaaccatg gcttccgcag tggcgacgcc gctggaaaaa
    13801 cagttttcca cggttcccgg cgtcgattcg atgagttcgg tgagcgcgct gggcgtcagc
    13861 cagatcacga tgcagttttc gctggatcgc aatattgatg cggcggcgca agacgtgcag
    13921 tctgcgattt cgctggcgca gaaacagctg ccgcctacga tgtcgactcc gccttcatac
    13981 cgcaaggtta acccggcgga cgcggcaatt ttctacctgg cgctgacttc cgacactctg
    14041 ccgctatcga cggtcgatga atacgcggaa acgctcatgg cgcagcgcat ttccatgatc
    14101 aacggggtgg cgcaagtgca ggtgttcggc gcgcaaaaat acgcggttcg cgtgcggctc
    14161 gaccccaatc tgctggcggc gaaaggcatc ggcatcgacg aagtggagaa agcgctcgac
    14221 cagcacaacg tcaatctgcc caccggcacg ttaaacggcc agcacagcac cgtcaccata
    14281 caggcaagcg gccaactaca ggacgccgcc gcctaccggc ccatgattgt cacttaccgc
    14341 aacggcgcgc cggtacggtt gcacgagctg ggccaggtca tcgacagcgt acagaacgac
    14401 aaggtggccg cttggtacaa cggggggcgc ggcatcgtgc tggccattca gcggcagcca
    14461 gggacgaata ccgtcgaagt ggtggacaac atcaagaaac tgctgcccga cttccgcgcc
    14521 cagatgccgg ccggggtcaa catggcggtg ctttacgacc ggtccgagtc catccgagct
    14581 tcggtcaatg acgtgcaatt ttcgctgttc ctggcgctgg ctctggtggt gatggtgatt
    14641 ttcctgtttt tgcgcaacct gccggcaacc gtgattccca gtctggcttt gccgttctcc
    14701 atcatcggca cgttctcggt catgtatttg ctgggctaca gcctggacaa cttgtccttg
    14761 atggcgctca cactatgcgt gggtttcgtg gtggatgacg cgatcgtaat gctggaaaac
    14821 attacccgcc acatggagag cggcgaaagc gcgttacagg ctacgctcaa tggctcgaaa
    14881 gaaatcgctt tcaccattct gtcaatgacc ttgtcgcttg ccgcggtgtt ctttccggtg
    14941 tttttcatgg gcggggtgct gggtaagctg ctgcatgagt ttgccgtgac catcatcact
    15001 gcggtgctga tttccggttt cgtgtcgctc acgctcacgc ccatgctgtg cagccgcctg
    15061 ctgcgtcctg cgcatcaaca gcacggccgc ctatacgcgg caagcgaagc ttttttcgag
    15121 ggcatgaagc acatttacga cataagcctg aagtggacgc tccgccacca acgcctcacc
    15181 cttgcggtgt ttgccgcgat tgttattgcc actggctggc tgtttgtaat aatgcccaaa
    15241 ggttttctac cgaacgagga caccggccag cttttcgtct tcaccgaggc ggcgcaggac
    15301 gtttccttcg aagccatggc caaacagcag caggccgtcg ccgacatcgt ccgcgccgat
    15361 cccaacgtgg catccatcat gtccttcatc ggcgcgagcg gctccagtca gtcgctgaac
    15421 ctgggacgca ttttcatccg cttgaagccg cgcggccaga gattgagcgc cgatgaggtg
    15481 attcaggaac tgcgaccgaa agtagaggca attcccggaa tcaaggtcta tctgcaaaac
    15541 ctgccgacca tccgcatcgg cggcatgctg agcaagagcc agtatcaata cacgctgcag
    15601 gacgccgata ccgaggtgct gtaccactgg gctccgctga ttgaagccaa gatcaggcaa
    15661 ttgcccggtt tccgcgacgt caccagcgac ctgcagattt ccaacccgca ggtgatggtg
    15721 gaaatcgacc gcgacaaggc ctctgcgctc ggcgtgacct cgcagcagat tgaaaacgcg
    15781 ctctacgacg cctatggggc gcgccaggtt tcgaccatct acacgccgag caacgagtac
    15841 tgggtcatca tggagctcga cccgaaatac caagccgacg ctcaagcgtt gtcgatgctc
    15901 tatgtgcgct caggcggtgg cacgctggtg ccgttaagct cggtcgcgcg gttgagcccc
    15961 ggagtgggca cgttgaccat caatcacctg gggcaattgc cggcggtgac catttccttc
    16021 gacctgcagc ccggggtttc tctcagcgac gcgctgcggc agatagagaa ggtgaagggc
    16081 gaattgggcg cgccggcgac gctcaacgcg agcttccagg gtgcagcgca ggcgttccag
    16141 gcgtcgctgc agaacatggg ctatctgctt gccatgtcga tattggtgat ttacctcgtg
    16201 ctcggcattc tctacgagag ctttgttcat ccgctcacca tcctttcggg cttgccgacc
    16261 gccggattgg gagcgctgat tacactgctg ctgttccatt acgatctcga tatgtacgct
    16321 tttgtcggca tcatcatgct ggtcggcatc gtcaagaaaa atgccatcat tatgatcgac
    16381 tttgcgatcg aggcgcagcg cgcgcacggg aagaagccct tcgacgcgat ttacgaggcg
    16441 tgcatcgtgc gcttccgccc gatcatgatg accaccatgg cggcgctgct ggccagcctg
    16501 ccgattgcgc tgggggtcgg tacaggttcc gaggcgaggc gcccgctggg g
//
LOCUS       DATIOW010000140         4257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_167448, whole genome shotgun sequence.
ACCESSION   DATIOW010000140 DATIOW010000000
VERSION     DATIOW010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167448"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VLL03_04665"
     CDS             complement(<1..255)
                     /locus_tag="VLL03_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HSS46690.1"
                     /translation="MSRSVELKDYQRELHYFKLRLAIAAGFVLFFFFLLFGRFFYLQV
                     LQHEHYHTLAENNRISVMPIVPNRGLILDRNGAVLAHNYSA"
     gene            complement(252..773)
                     /gene="mreD"
                     /locus_tag="VLL03_04670"
     CDS             complement(252..773)
                     /gene="mreD"
                     /locus_tag="VLL03_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006682010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HSS46691.1"
                     /translation="MSVPAARAHEILSPVKPGLIAVSLIAALFFNLLPWPGVMLFVRP
                     DFVALTLLYWCIHRPHKVGFWTAWFMGLLMDIADGAVFGQHALAYALLAYLAILLHRR
                     VLMFGLRKQIWHVLPVLLLTQMAILLIKIYGGANFIGWGVFLSSITGALLWPALSILL
                     LLPQRPKPDPEEI"
     gene            complement(785..1690)
                     /gene="mreC"
                     /locus_tag="VLL03_04675"
     CDS             complement(785..1690)
                     /gene="mreC"
                     /locus_tag="VLL03_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HSS46692.1"
                     /translation="MDTQLPPFFKRGPSPFIRLVIFTLLSLLLLSTDARFKYLDAGRA
                     ALSVLIYPLQKLGSTPGYLLGQASDFFATQSYLTAENARLNEKNLANAAMLQRYQALQ
                     EENNHLRRLLNTRERFQGNATLAEILYTGRDLFSRRIVIDKGMQNSVKPGQAVVDDIG
                     VIGQVTRVYPFVSEVTLITDKEQTVPVEVARNGLRAVLFGAGEPGELELRYMPVNVEI
                     QNGDELVTSGIDGLYPRGLPVAVVTKIERNSAYAFARISCIPKAGVDRHRQILVLTLE
                     PKIADNPLEQPAPRSAGKSGKKRFN"
     gene            complement(1800..2843)
                     /locus_tag="VLL03_04680"
     CDS             complement(1800..2843)
                     /locus_tag="VLL03_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HSS46693.1"
                     /translation="MLGFLKGYFSNDMAIDLGTANTLIYVRGKGIVLNEPSVVAIRQD
                     RGPNGKKVIQEVGLAAKQMLGRTPGNITAIRPMKDGVIADFTITEQMLKQFIKKVHDS
                     RLFSPSPRIIICVPCGSTQVERRAIRESAIGAGASKVYLIEEPMAAAIGAELPVAEAT
                     GSMVVDIGGGTTEVGVISLGGIVYANSVRVGGDKFDEAIINYIRRNYGMLIGETTAET
                     IKKEIGSAFPGSEVREMEVKGRNLAEGIPRSFTISSNEILEALTDPLNSIVSAVKSAL
                     EQTPPELGADIAEKGMVLTGGSALLRDIDRLLMEETGLPVIVADDPLTCVVRGCGRAL
                     EKMDKLGNIFADD"
     gene            2989..3275
                     /gene="gatC"
                     /locus_tag="VLL03_04685"
                     /pseudo
     CDS             2989..3275
                     /gene="gatC"
                     /locus_tag="VLL03_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264711.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
     gene            3377..>4257
                     /locus_tag="VLL03_04690"
     CDS             3377..>4257
                     /locus_tag="VLL03_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HSS46694.1"
                     /translation="MLNSSLKQLSGLLAEKKISSVELTREFLSRVAKLNPELNAFITL
                     NEEVSLREARAADVRIAQDRAQPLTGIPIAHKDIFCAKGWLTTCGSKILSNFVAPYDA
                     HVIERCREVGMVTLGKTNMDEFAMGSSNETSYYGPVKNPWDVRTVPGGSSGGSAAAVA
                     ARMAPVATGTDTGGSIRQPAALTGISGIKPTYGMVSRYGMIAFASSLDQGGPLAKSAE
                     DLALMLNVIAGFDARDSTSLNRPREDYTRDLARPLAGLKIGLPREYFAEGLSSDVACA
                     VEAAIAKYKKLGATTVG"
BASE COUNT          907 a         1216 c         1274 g          860 t
ORIGIN      
        1 ggcggaataa ttgtgcgcca gcaccgcgcc gttgcggtcg agaatcagcc cgcggttggg
       61 gacgatgggc atcacggaaa tgcggttgtt ttcggcgagc gtgtgatagt gctcgtgctg
      121 gaggacttgc aggtaaaaaa aacggccaaa cagcagaaag aaaaaaaaca gcacgaagcc
      181 cgccgcaatc gcgagccgca gcttgaaata atggagctcg cgctgataat ccttgagctc
      241 gacgctgcgg ctcatatctc ttcaggatcg ggcttggggc gttgcggaag caaaagcaat
      301 atcgaaagcg ccggccacaa caacgcgccg gtgatgctgc tcaggaaaac cccccagccg
      361 atgaaattcg cgccgccata aattttgatg aggagtatgg ccatctgggt cagcagcaat
      421 accggcaaaa catgccagat ctgcttgcgc aacccgaaca tcagcacgcg gcgatgcagc
      481 agaatggcga gataggcgag cagcgcgtaa gcgagcgcgt gctgaccgaa caccgcgccg
      541 tcagcgatat ccatcaataa tcccatgaac cacgccgtcc agaaaccgac cttgtgcggc
      601 cggtgaatgc accagtagag cagggtgagc gcgacgaaat cggggcgcac aaaaagcatc
      661 acgcccggcc acggcagcag gttaaaaaaa agcgccgcaa tcagggacac ggcgatcaag
      721 cccggcttga ccggcgagag aatctcatgg gctcgggcgg caggtaccga cattaagata
      781 ggtcctagtt aaacctcttt ttcccgcttt tgcctgcaga tcgcggcgcg ggctgctcca
      841 atggattgtc ggctattttt ggctccagcg tgagcaccaa tatctggcgg tgacggtcca
      901 cgcccgcttt cgggatgcag ctgatacggg cgaatgcgta cgcgctgttg cgctcgattt
      961 tggtcaccac cgcgaccggt aggccgcgcg gatataagcc atcgatcccc gaagtcacca
     1021 actcatcgcc gttctggatt tccacgttga ccggcatgta gcgcagttcg agttcgcccg
     1081 gttcgcccgc gccgaacagc accgcgcgca ggccgttgcg cgccacttcc accggaaccg
     1141 tctgctcctt gtcggtaatc agcgtcactt cgctcacgaa agggtaaacc cgcgtgacct
     1201 ggccgatgac cccgatatcg tcgaccaccg cttgccccgg cttaacgctg ttttgcatgc
     1261 ccttgtcaat gacgatgcgg cgtgaaaaca aatcgcggcc ggtgtagagg atttccgcca
     1321 gcgtggcgtt gccctgaaag cgctcgcggg tattcagcag ccgccgcaaa tggttgtttt
     1381 cttcctgtag cgcctggtaa cgctgcagca tcgccgcatt ggcgaggttc ttctcgttta
     1441 atcgggcatt ctcggccgta agataggatt gggtggcaaa gaaatcactg gcctgcccaa
     1501 gcagatagcc cggcgtgctg ccgagctttt gcagcgggta gatgagtacc gaaagtgcgg
     1561 cgcgccccgc atcgaggtat ttgaagcggg catcggtgga gagaagcagc agggacagca
     1621 gagtgaaaat caccaggcgg ataaaaggac tgggaccgcg tttgaaaaat ggcggaagct
     1681 gggtgtccat ggatcagcct tcagctatca gccatcataa gccatcaaca ctcagcgttc
     1741 agcatgtcag gggcggaaca gggtggagct gaaagctgat ggctgatagc tgaagtccat
     1801 cagtcatctg cgaaaatatt gccgagcttg tccattttct ccagcgcccg tccacagccg
     1861 cgcactacgc aggtcagcgg atcgtcggcg acaatcaccg gcaagccggt ttcctccatc
     1921 agcagacggt cgatgtcgcg cagcagcgcg ctgccgccgg tcaacaccat gcctttttcc
     1981 gcgatgtctg cgccaagctc cggcggtgtc tgctccagcg cggatttgac cgcgctgacg
     2041 atgctgttga gcggatcggt gagtgcttcc aggatttcgt tgctggaaat ggtgaagctg
     2101 cgcgggatgc cttcggcgag gttgcgtcct ttcacttcca tctcgcgcac ctcgctgccg
     2161 gggaaggcgg aaccgatttc cttcttgatc gtctcggcgg tggtctcccc gatcaacatg
     2221 ccgtagttgc ggcggatgta gttgatgatg gcctcgtcga acttgtcccc ccctacccgc
     2281 accgaattgg cgtagacgat gccgcccagg gaaatcacgc cgacttcggt ggtgccgccg
     2341 ccgatatcga ctaccatcga gccggttgct tcggccaccg gcagctccgc gccgatggct
     2401 gccgccatcg gctcttcaat caggtaaacc ttgctggcgc cggcgccaat cgccgattcg
     2461 cggatcgccc ggcgttccac ctgggtcgag ccgcagggca cgcagatgat gatgcgcggg
     2521 gaggggctga acagccgcga gtcatgcact ttcttgatga actgcttgag catttgctcg
     2581 gtgatggtga aatcggcgat cacgccgtcc ttcatcggcc ggatggcggt aatgttgccg
     2641 ggggtgcggc cgagcatctg tttcgccgcc agcccgactt cctgaatgac tttcttgccg
     2701 ttgggccccc tgtcctggcg tatggccacg accgagggtt cattcaagac gatgcccttg
     2761 ccgcggacgt aaatcagggt gttggcggtg ccgaggtcaa tcgccatatc gttggaaaag
     2821 taacctttta ggaatccaag catgtctaaa gtcccgtttt ctagggtaag gtgcgccagt
     2881 caaataagtt ataatacctt ttatgctcgc aggtttaaag ctccctgagg ccggtttttc
     2941 ggagcccctg cctgcctgcc cccaatgtga ctcaaaacgc tctgaaccat gccgctttcc
     3001 ctggatgatg taaagcgcat tgcgcacctt gtcgcctcga ggtctctgag cccgaggcgg
     3061 ccacggtgct caagcagctt tccggaattt tcgcgctgat tgaagaaatg ctggcggtgg
     3121 acaccagcgg catcgaacca atgccgcacg ctcaggatgt ggtactgagg ttgcgcgaag
     3181 atgcggtgac cgaatccgac cagcgcgagc tgttccagtc cgtcgccccg caagtggaag
     3241 cgggcttgta tctggtgcca aaagtaatcg aatgatgagt gaatgaggtg aaagagtgaa
     3301 gaagtgaata gagaacacct cagccacttg cttcaccgcg cgcagcgcga ctcactcctt
     3361 cacttgaatt ttccgaatgc ttaactccag tttaaaacaa ttgtccggtc ttctggccga
     3421 gaaaaaaata tccagcgtcg aactcacccg tgaatttctc tcgcgcgtcg cgaaactcaa
     3481 tcctgaattg aacgcgttca tcacgctgaa cgaggaagtg agtctgaggg aagcgcgagc
     3541 cgcggacgtg cggattgccc aagaccgggc gcagccgctc accggcatac cgattgcgca
     3601 caaggacatc ttttgcgcca aaggctggct gaccacttgc ggctcgaaaa ttctctccaa
     3661 cttcgttgca ccctacgatg cgcatgttat agagcgctgc agagaggtgg ggatggtcac
     3721 tttaggcaaa accaacatgg atgaattcgc catggggtca agcaacgaga cttcttatta
     3781 tgggccggtg aagaacccgt gggacgtgag aacggtgccg ggaggaagct cgggtggttc
     3841 ggctgcggcg gttgcggcgc gcatggcgcc agtcgcgacc ggcaccgata ccggcggttc
     3901 gatccgccag ccggcggcac tcaccgggat ttccggcatc aagccgacct acggcatggt
     3961 ctcgcgttac ggcatgattg cgtttgcttc cagcctggat cagggtgggc cgctggcgaa
     4021 aagcgcggaa gacctggcgc tcatgctcaa cgtgatagcg ggatttgatg cccgcgattc
     4081 caccagcctc aaccgtcccc gggaagatta cacccgcgat ttggccaggc cgctggctgg
     4141 tttgaagatt ggtctgccca gggaatattt tgccgaaggc ttgagcagtg atgtagcgtg
     4201 cgcggtggag gctgcaatcg ccaaatacaa aaagcttggc gccaccacgg tcgggat
//
LOCUS       DATIOW010000141         4438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_676064, whole genome shotgun sequence.
ACCESSION   DATIOW010000141 DATIOW010000000
VERSION     DATIOW010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676064"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1585)
                     /gene="rng"
                     /locus_tag="VLL03_04695"
     CDS             complement(131..1585)
                     /gene="rng"
                     /locus_tag="VLL03_04695"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HSS46695.1"
                     /translation="MSEDILINVTPQETRVAVMRQGVVQELHVERVSSRGLVGNIYVG
                     EVCRVLPGMQSAFVEIGLERAAFLHVADIWVQHQNGDRDQHIEKLLHEGQKLLVQVTK
                     DPISTKGARLTTQISIAGRMLVYLPQESYIGISQKIEDEEEREHLRQKMQQLLPQESS
                     GGFIIRTMAETASERELLADIEYLRKLWSGIQEKSQSAPPRTLLYQDLTLSLRVLRDF
                     VSEETGRIVADSRETFDKMCAFAEDYMRNAKQRLEHYTGERPLFGLHGVEDEIEKALA
                     RRVDLKSGGYLIIDQTEALTTVDVNTEGFVGGRNFDDTIFKTNLEAAHIIARQLRLRN
                     LGGIIIIDFIDMDSEEHRNAVLAEFKKALACDRTRMTVNGFTALGLVEMTRKRTRESL
                     AHVLCEPCPTCEGRGEVKTAQTVCYEILREVLREAREFSAREYRILASQEVIDLFLDE
                     ESQSLAMLSDFIGKPISLQVETQYPQEQYDIVLM"
     gene            complement(1582..2202)
                     /locus_tag="VLL03_04700"
     CDS             complement(1582..2202)
                     /locus_tag="VLL03_04700"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HSS46696.1"
                     /translation="MVLDVKCIYLASHSPRRRELLKQIGVNFEVVQFREFLARGPDVD
                     EAPLAGEPPENYVKRIACAKTEAGWSCLVQRRLRRFPVLAADTTVVLDGKIIGKPVSR
                     DHVVAMLSELSGREHSVLTAVALKFEDKTDLGLSTTLVRFKQLAEEEVRHYAATGEAL
                     DKAGGYAIQGLAAAFVAGIDGSYSGVMGLPLFETAQLLREFGYGVL"
     gene            complement(2701..3171)
                     /gene="rlmH"
                     /locus_tag="VLL03_04705"
     CDS             complement(2701..3171)
                     /gene="rlmH"
                     /locus_tag="VLL03_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HSS46697.1"
                     /translation="MKLWIIAVGNKMPAWVDMSFCEYAKRMPPETKIQLVEIKAEKRS
                     AGKSLNQVLDAERGRILASLPRHCRQVVLDEGGRALSTAQLAQNLAAWMQAGRDVAFV
                     IGGADGLHEYIKNNADNRLSISAMTLPHALVRVVLAEQLYRAVSLMNNHPYHRR"
     gene            complement(3181..3540)
                     /gene="rsfS"
                     /locus_tag="VLL03_04710"
     CDS             complement(3181..3540)
                     /gene="rsfS"
                     /locus_tag="VLL03_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HSS46698.1"
                     /translation="MRLDKMVKATVAALEDIKARDIVVLDVRPMTSLFNWMVIASADS
                     ARQTKALSNNVREKLKSFGAKVYGVEGQENGEWVLVDLGDVVVHIMQPAVRKYYNLEQ
                     LWGYSRPVRRGRKTSAF"
     gene            complement(3537..4196)
                     /gene="nadD"
                     /locus_tag="VLL03_04715"
     CDS             complement(3537..4196)
                     /gene="nadD"
                     /locus_tag="VLL03_04715"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HSS46699.1"
                     /translation="MASELKPIGVFGGAFDPVHYGHLALARETADRLDLTEVRFVPAN
                     MPPHRAQPHASAEQRVQMLKLAISGNPCFVLDQREILREGASYTVDTLSAMRSEWGGK
                     RPLCLLMGADTFAGLASWHRWKKLFELAHIVVVQRPGFDLRHLQSSLEEEFAKRRAKS
                     PHASHDKPCGAIIVVEVTPVDISASMIREKLAGGKSVRNLLPGLVLDYIQAQGLYHGG
                     G"
     gene            complement(4206..>4438)
                     /locus_tag="VLL03_04720"
     CDS             complement(4206..>4438)
                     /locus_tag="VLL03_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HSS46700.1"
                     /translation="GIGGTAFTPIINAPEVAILGVSKAQLKPVYKDGQFAPRLILPLA
                     LSYDHRVIDGASAARFTTYLCSVLSDIRRLLL"
BASE COUNT          887 a         1365 c         1234 g          952 t
ORIGIN      
        1 gcgaggaacg cagggaactc ctgagcttgt cgaaggagcg gcgaggcagg gcgtcctttt
       61 gtttggttac tttattttgg acgagcaaaa taaagtaacc agccgccggt ctgctaccgg
      121 caaatttaat tcacatcaac acaatatcgt actgctcctg cgggtactgt gtttctactt
      181 gcaggctgat cggcttgccg ataaagtcgc tcaacatggc aagactctgc gattcctcgt
      241 cgaggaacaa atcaatcact tcctgcgagg cgagaatacg atactcacgg gcactgaact
      301 cgcgcgcctc gcgcagtacc tcgcgcagga tttcataaca aacggtctgc gcggtcttca
      361 cttcgccgcg tccttcacaa gtgggacagg gctcgcacag cacgtgcgcg agactttcgc
      421 gggtgcgttt cctggtcatt tcaaccagtc ccagcgcggt gaagccgttt actgtcatgc
      481 gcgtgcggtc gcacgcgagg gctttcttga actcggcaag caccgcgttc cggtgttctt
      541 cgctgtccat gtcaatgaag tcaataatga tgatgccccc gaggttgcgc agcctcagtt
      601 gcctggcgat gatatgtgcc gcttccaggt tggtcttgaa gatggtgtcg tcaaaattgc
      661 gtccgccgac gaacccttcg gtatttacgt ccacggtggt aagcgcctcg gtctggtcta
      721 tgatgagata acctccggac ttcaaatcca cgcgccgcgc cagcgccttt tcgatttcgt
      781 cttccacccc gtgaagacca aagagcgggc gctctccggt atagtgctcc agccgctgtt
      841 tcgcgttacg catgtaatcc tcggcgaacg cgcacatttt atcaaaggtc tcgcgcgaat
      901 ccgccacgat gcgcccggtt tcctcgctga cgaaatcgcg caaaacacgc aggcttaagg
      961 tcaagtcctg gtagagcaaa gtgcgcggcg gcgcggattg tgatttttcc tgaatgccgc
     1021 tccatagttt gcgcagatat tcgatgtcgg caagcagctc gcgctcgctc gcggtttccg
     1081 ccatggtgcg gatgatgaat ccgccgctgc tctcctgcgg cagcagttgc tgcattttct
     1141 ggcgcaaatg ctcccgctct tcctcgtcct cgattttctg cgaaatgccg atgtacgatt
     1201 cctgcggaag ataaaccagc atgcggcctg caatgctgat ctgggtggtg aggcgcgctc
     1261 ccttggtgct gatcgggtct ttggtcacct gcaccaagag cttctgccct tcatgcagca
     1321 gtttttcaat gtgctggtcc cgatcgccat tctggtgctg tacccagata tccgcgacat
     1381 gcaggaatgc cgcgcgctcc aggccgattt ccacgaaagc cgactgcatg ccgggcagca
     1441 cccggcacac ttcgccaaca taaatgttgc cgaccaatcc ccggctgctc acgcgctcga
     1501 catgcagctc ctgcaccacc ccctgccgca tgaccgcgac gcgcgtttcc tggggtgtga
     1561 cgttaatcag aatgtcttcg ctcacagcac gccgtatcca aattcccgca gcaattgcgc
     1621 ggtttcgaat aacggcaatc ccattactcc ggaataactg ccgtcgatcc cggcaacgaa
     1681 agccgcggcc aagccctgta tcgcgtagcc gcccgcctta tccagtgctt caccggttgc
     1741 ggcatagtgg cggacttcct cttcggcaag ctgcttgaag cgcaccaaag tggtggacag
     1801 acctaggtcc gttttgtcct caaacttcag cgccaccgcg gtcagtacac tatgttctct
     1861 tcctgaaagc tcgctcagca tggccacgac atggtcgcgg ctgaccggct tgccaatgat
     1921 tttcccatcc agcaccactg tggtgtcggc ggccagcacc gggaaacgcc gcagcctgcg
     1981 ctgtacgaga cacgaccagc ctgcctccgt cttggcgcaa gcaatgcgct ttacgtagtt
     2041 ttcagggggc tcgcccgcga gcggcgcctc gtccacatcg ggcccgcgcg ccaagaactc
     2101 cctgaattgc accacttcaa aattcacccc gatctgtttc agcagctcgc gccggcgcgg
     2161 gctgtgcgaa gcaaggtaaa tacacttaac gtcgagcacc atgtttgagg aacccctgat
     2221 taagtcctcg tgaattgcgt tgctggcaaa atgcggatgg atgcaaggcg cgcaacgaag
     2281 gtcgtactcg aagtacgaca ccgaggagcg caacgccgca gacaccgcat tttaccgcaa
     2341 cccggaggga cggctgccgc gacacggtca gcgaagcttg gggtgcggga gcggcagcat
     2401 gagcgcccat tttacaatgt ttcttgcagc agcttgcgcc ccttcccgca ccacgctgcg
     2461 cgagtccgag tccggggcgc cttatccggg cggtctgctt tgacgctcgg cttgccaagg
     2521 gggtctggcc attggctgca cctcgcttcg cgtgtgcgct cgcggaccgt cgcgcctcgg
     2581 aacgtgcgca ggggagcgag cgccgcgccg cctctgggtc gcactgccga ggctccgcct
     2641 gcggcggcgc tatcccggaa agtcaccgtc gcaaccgcgt gggactcaat caggggttcc
     2701 ttatctgcgg tgatacggat ggttgttcat caaggacacg gcccggtaga gttgctcggc
     2761 cagcaccacg cgcaccagag catgcggcag ggtcatcgcg gaaatcgaca accggttatc
     2821 tgcattgttc ttgatatatt cgtgcagccc gtcagcgccg ccgatgacga atgccacatc
     2881 acgccccgcc tgcatccagg ccgcaagatt ttgcgcaagc tgcgcggtgc ttaaggctcg
     2941 cccgccttca tccagcacta cctgccggca gtgacgcggc agggaggcga gtatgcgtcc
     3001 gcgctcggca tccaggacct ggttcaggga tttcccggcg ctgcgctttt ccgctttgat
     3061 ttcgacgagc tgaattttgg tctcgggtgg catgcgcttc gcgtattcgc aaaagctcat
     3121 gtcaacccag gccggcattt tattgcctac cgctataatc cacagcttca tcgctgtaca
     3181 ctagaaggcg gaggtcttgc ggccgcggcg caccggccgg gaataacccc acaactgttc
     3241 caggttatag tacttgcgca ccgcgggctg cattatgtgc accaccacat cacccagatc
     3301 caccagcacc cattcgccgt tttcttgccc ttccacgccg tatacttttg caccgaagga
     3361 tttaagtttt tcccgcacgt tgttggaaag cgccttggtc tgccgcgcgg agtctgcgct
     3421 tgcgatgacc atccaattga ataaactcgt catggggcgc acgtcgagca ccacgatatc
     3481 ccgcgccttg atgtcttcca gcgctgcgac tgtggccttg accattttat cgagtctcat
     3541 ccgcccccat gatataaacc ctgtgcctga atataatcga gaaccaagcc tggaagcaaa
     3601 ttgcgcacgc ttttgccgcc agccaacttc tccctgatca tcgaagcgga aatatccacc
     3661 ggcgtgactt ccacaacaat aatggcgcca catggcttgt cgtgtgaggc gtgcggcgat
     3721 ttggcgcggc gctttgcaaa ctcttcctcg agcgacgatt gcaaatgcct tagatcaaag
     3781 ccggggcgct ggacaaccac gatgtgcgcc aactcaaaca gcttcttcca gcggtgccat
     3841 gaggcaagcc cggcaaaggt atccgctccc atgagcaggc acagcggtct tttaccgccc
     3901 cactccgagc gcattgcgct cagcgtatcc acggtataac tggcgccttc gcgcagaatc
     3961 tcgcgctggt ccagaacgaa acagggattg ccggaaatcg caagtttgag catttgcacg
     4021 cgctgctcag ccgaggcatg tggctgagcg cggtgtggcg gcatgtttgc cgggacaaaa
     4081 cgcacctctg tgagatcaag gcggtcggcg gtttccctgg caagcgcaag atggccgtaa
     4141 tgtaccggat caaacgcgcc gccgaaaaca ccgattggtt ttaattcact cgccacagag
     4201 gaaaatcaaa gcagcaagcg acggatgtcg gaaagcaccg agcaaagata ggtagtgaag
     4261 cgcgcagcgc tagcgccgtc gattacccgg tgatcgtaag agagtgccag cggcaaaatc
     4321 agcctgggcg cgaactggcc atctttgtat accggtttga gctgcgcctt ggatacgccg
     4381 agaatcgcca cttcgggggc gttgataatg ggcgtgaacg cggtaccgcc gatgccgc
//
LOCUS       DATIOW010000142         4908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1285370, whole genome shotgun sequence.
ACCESSION   DATIOW010000142 DATIOW010000000
VERSION     DATIOW010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285370"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="VLL03_04725"
     CDS             complement(<1..1080)
                     /locus_tag="VLL03_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46701.1"
                     /translation="MILILNDNVKTDSAEYKQLLEHLAALPNIKCRVHREQGEERALT
                     EVYLIGNTSTLQVEDMKNLPCVERVVRVSEEYRVLGRHKDDRRPTWFDYNGVRFGQDT
                     LHIFAGLCAVDTREHVEMMMKALKEHGQVCTRMGAYKPRTSPYSFQGHGKNCLPYVFE
                     FAGKYGIRVIAMEITHDLQVDEIREALRLTGNPTGVMLQIGTRNTQNFELLKSVGRQQ
                     DFPVLLKRGFGITLDESLNAAEYLASEGNRKVVFGLRGMKTNMGDPHRNFVDFSHVPV
                     VKRLTRMPVCIDPSHSVGSRSVAPDGILELMHVAVQGIIAGANMILVDFHPVPSRALV
                     DGPQALLLKELPQFIEDVQIAREAYL"
     gene            complement(1250..1645)
                     /locus_tag="VLL03_04730"
     CDS             complement(1250..1645)
                     /locus_tag="VLL03_04730"
                     /inference="COORDINATES: protein motif:HMM:NF038027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TssQ family T6SS-associated lipoprotein"
                     /protein_id="HSS46702.1"
                     /translation="MLKPRPWLYATAAVLLCAGCVGPLKDMNPMTLIQGKGAPALSEG
                     IKSYDDGEYDKAQKSLNGALEQGLSLKSDQVKAHKYLAFTYCVNKREHQCREEFKKAL
                     EINPDFELEPTEAGHPLWGPVFRSVKTRK"
     gene            complement(1660..2691)
                     /locus_tag="VLL03_04735"
     CDS             complement(1660..2691)
                     /locus_tag="VLL03_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSS46703.1"
                     /translation="MVFADIVEYSKKPVAEQIKLKDQFNGIISRAIIRTSPNDRIILD
                     TGDGVAISFLGAPEDALFVGLSLRDSLKEIESANELRARIGINLGPVRVVKDINGQPN
                     IIGDGINVAQRVMSFAEPNEVLVSRSFYEVVSHLSQEYELLFSYQGARADKHVREHEV
                     YAVGTPGKDIRPWLGRARVHGEGAAEAVDDKERAALTGDTPAKAKGGAGKKLLYAGVL
                     LVMIAALAFALRGSKPAPETSKPLSATEAKPRKSVPMAEAAKPATLTPAPAPAAAPAG
                     GLIALAIKPWGEIYVDGKKMGVSPPLKELPLTPGKHTIEIKNGAFPTYTETVEVEHNG
                     RVKIVHQFR"
     gene            complement(2826..4286)
                     /gene="tldD"
                     /locus_tag="VLL03_04740"
     CDS             complement(2826..4286)
                     /gene="tldD"
                     /locus_tag="VLL03_04740"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HSS46704.1"
                     /translation="MHTETAITSLETANRFLLAPYDLDHAKLERVFGNLLSHQIDYAD
                     LYFQITRSESWALEEGSVKSGSFNIDQGVGVRAVSQDKTAFAYSDDISLAALESAAQA
                     TRAIARQGAQSRTQVAKRSRGRNLYSPCDPLASIPDTHKVTLLEKVEKHARSIDPRVT
                     QVIAYLGGEYEVILVARSDGMTAADVRPLVRLSLQVIAEEKGRREQGTGGGGGRFDYA
                     YFTDEILEGYCQQAVSQALTNLRARPAPAGAMTVVLGSGWPGILLHEAVGHGLEGDFN
                     RKGSSAFSGRIGERVAAPGVTVVDDGTIARRRGSLNIDDEGNPTQRTVLIENGVLRGY
                     MQDMLNARLMGAAPTGNGRRESYAHIPMPRMTNTYMLNGDKHPEEIIKSVKRGLYAVN
                     FAGGQVDIVSGKFVFSAAEAYLIQDGKVGQPVKGATLIGNGPDVLTRVSMIGNDMALD
                     PGIGTCGKEGQSVPVGVGLPTLCIEGLTVGGTLEPV"
     gene            complement(4320..>4908)
                     /locus_tag="VLL03_04745"
     CDS             complement(4320..>4908)
                     /locus_tag="VLL03_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HSS46705.1"
                     /translation="KKHKIWLAGGSVPLAASVPTKVRNSCLLYDDKGKMAARYDKIHL
                     FGLDLGNEHYHEQATIEPGNEVVVAETPFGRVGLSVCYDLRFPELYRAMGNVDIILVP
                     AAFTETTGKAHWETLIRARAIENQAYVLAPAQGGYHVSGRETHGNSMIVDPWGVILDR
                     LARGSGVVMAGVNPQYQASLRKSLPALTHRTLRRV"
BASE COUNT          946 a         1520 c         1342 g         1100 t
ORIGIN      
        1 taggtaggct tccctggcga tttgcacatc ctcgataaac tgcggcaatt ccttgagcaa
       61 cagcgcctgc gggccgtcca ccagcgccct actcgggacc ggatgaaaat ccaccagaat
      121 catgttggcg ccggcaatta tgccctgcac cgcgacatgc atgagctcga gaatgccgtc
      181 gggcgcgacg ctgcgcgagc ccaccgaatg cgacgggtcg atacacaccg gcatgcgcgt
      241 caagcgcttc accaccggca cgtgtgagaa gtcgacaaaa ttgcggtgcg gatcgcccat
      301 gttagttttc attccgcgta aaccaaacac caccttgcgg ttgccttccg aagccaaata
      361 ctcggcggcg ttgagcgatt cgtcgagcgt gatgccaaaa ccacgtttca acagcacggg
      421 aaaatcctgc tggcgaccga cgcttttgag caactcgaag ttctgggtgt tgcgcgtgcc
      481 gatttgcagc atgacgccgg tcgggttccc ggtcagacgc aaagcctcgc gaatttcgtc
      541 gacctgcaaa tcatgggtga tttccatggc aatcacccgg atgccgtact tgccggcaaa
      601 ctcgaacacg tacggcaggc aattcttgcc gtgaccctgg aacgaatacg ggctggtgcg
      661 cggcttgtat gcgcccatgc gcgtgcatac ctggccatgc tctttcagcg ctttcatcat
      721 catttccacg tgctcgcggg tatccacggc gcacaagccg gcgaagatat gcagcgtgtc
      781 ctggccaaag cgtacgccgt tgtagtcaaa ccaggtcggg cgccggtcgt ccttgtgccg
      841 gccgagtacg cggtattcct cggacaccct caccacacgc tcgacgcacg gcaggttctt
      901 catgtcctca acttgcagcg tactggtatt gccgatgaga taaacttcgg tgagcgcccg
      961 ttcttccccc tgctcgcggt gcaccctgca cttgatattg ggaagcgccg caagatgttc
     1021 cagcagctgc ttatattccg cgctgtcggt ttttacgttg tcgttcagga taagaatcat
     1081 gtttgagccg gttgggattc gatgaaggag ccgcccgcct cgaggcaatc ctcagtggcg
     1141 gcagagttaa gcggggtatt ttaacgagta aagatgtcgc gcacattccg cggtatgcct
     1201 tgactccggt cgggtcaaac ccttcgggcc ggacagcggc gagtaatttt tattttcggg
     1261 tcttgacgct gcggaacacc ggcccccaca agggatggcc cgcttcggtc ggttcaagct
     1321 cgaagtcggg attgatttcc agcgctttct tgaactcctc ccggcactga tgctcgcgtt
     1381 tgttgacgca gtaagtgaag gcgagatact tatgcgcttt cacctggtcg gatttcagcg
     1441 acaacccctg ctccagcgcg ccgttgagac ttttttgcgc cttgtcgtat tcgccgtcat
     1501 cgtaactctt gattccctcc gaaagcgcgg gagcaccctt gccttgaatc aaggtcatgg
     1561 gattcatatc ttttaacggt ccgacgcagc ctgcgcacag cagaacggct gcagtcgcgt
     1621 acagccatgg tctcggctta agcatggttc ccctcctcgt tagcggaact gatgcacaat
     1681 cttaacgcgt ccgttgtgct cgacctctac tgtttcagta taagtcggga aagcgccgtt
     1741 ttttatttcg atagtatgct tccccggcgt gagcggcagt tccttgagcg gcgggctgac
     1801 gcccatcttc ttgccgtcca catagatttc accccacggc ttaatcgcca gcgcgatcag
     1861 cccgccggcc ggagccgctg cgggagccgg cgcgggcgta agtgtagcag gcttggcagc
     1921 ttctgccatt ggcacagact tcctgggttt ggcttctgtc gcggacagcg gcttagaggt
     1981 ttccggcgcg ggcttgcttc cgcgcagcgc gaacgccagt gcagcaatca tcaccagcag
     2041 cacgcccgca taaagcagct ttttgccggc cccccctttg gctttggccg gcgtgtctcc
     2101 ggtcagcgca gctctttctt tgtcgtcaac ggcttcagca gcgccttctc catgcactct
     2161 tgcccttccc agccatggcc ggatgtcttt gcccggcgtg ccgaccgcat agacttcgtg
     2221 ttcgcgcaca tgcttgtcgg cgcgcgctcc ctgataggaa aacagcagct cgtattcctg
     2281 ggaaagatgc gagaccactt cataaaacga gcgcgacacc agtacttcgt tgggctcggc
     2341 aaagctcatc acgcgctgcg cgacgttgat gccgtcgccg atgatgttgg gctggccgtt
     2401 gatgtccttg accacgcgca ccggcccgag attgatgccg atgcgcgcgc gcagctcatt
     2461 ggcactttca atttctttca aagaatcgcg cagacttaat cccacaaaca acgcatcttc
     2521 cggcgccccc aggaaactga tcgccacccc atcgccggta tcgaggatga tgcgatcgtt
     2581 gggtgatgtc ctgatgatgg cacgggaaat gataccgttg aactggtcct tgagcttaat
     2641 ctgctcggcg accggcttct tcgaatattc gacgatatcc gcgaacacca cgctgcagat
     2701 aaaagttctg ttccccctgt cgagtatttc ttgtccgtta atgtttagct cctcagaacc
     2761 tgtttatata gcctttcacg tgacgcgttg ctggccagaa atagatgagt gcaggcgcga
     2821 cgtcattaaa ctggttctag cgtgccgccc accgtcaatc cttctatgca cagcgtgggc
     2881 aaacccacgc cgactgggac gctctgccct tccttgccgc aagtgccgat gcccgggtcg
     2941 agcgccatgt cgttgccgat cattgaaaca cgggtgagca catccggccc gttgccgatc
     3001 agcgtggcgc ccttcaccgg ctgaccaacc ttgccgtcct gaataagata cgcctcggcg
     3061 gcggaaaaca cgaacttgcc gctcacgata tccacctggc cgccggcgaa attcaccgca
     3121 tacagcccgc gtttcaccga ttttatgatt tcctccgggt gtttgtcacc gtttaacata
     3181 taagtgttgg tcatgcgcgg catcgggata tgggcgtagg attcgcgccg tccgttgccg
     3241 gtgggggccg cgcccatgag ccgcgcgttc agcatatcct gcatatagcc gcgcagcacg
     3301 ccgttttcaa tcagcaccgt acgctgcgtt ggattgcctt catcgtcaat gttgagcgag
     3361 ccgcggcgcc tggctatcgt gccgtcatca accacggtaa cacccggtgc ggcgacacgc
     3421 tcgccgatgc gcccgctgaa agcggaactt cccttgcggt tgaaatcgcc ttcaagcccg
     3481 tgcccaacag cttcgtgcag cagaatcccc ggccagccag agcccaacac caccgtcatt
     3541 gctcccgccg gcgctggcct ggctcgcaag ttggtgagcg cctgggacac cgcctgctga
     3601 caatagcctt ccaggatttc gtcggtgaaa taagcataat caaaacgtcc accaccacca
     3661 cccgtgccct gctcgcgccg ccccttctcc tcggcaatca cctgcagcga caggcgcacc
     3721 agcggacgca catccgccgc cgtcatgccg tcgcttcgcg ccaccaggat gacttcgtat
     3781 tcgccgccca gatatgcaat cacctgggtt acgcgcggat cgatgctgcg cgcatgtttt
     3841 tcaactttct ccagcagcgt cactttgtgc gtgtcgggaa tgctcgccag cgggtcgcag
     3901 ggtgaataaa gattacgccc tctactgcgt ttggcgactt gtgtacgcga ttgtgcgccc
     3961 tggcgcgcga tcgcccgcgt tgcctgcgcg gcgctttcca gcgcagcgag gctgatgtcg
     4021 tcggaatagg caaacgcagt cttgtcctgc gacaccgcgc gcacgcccac gccctgatcg
     4081 atgttgaagc tgccggactt cacgctgcct tcctccaatg cccaggattc gctgcgggtg
     4141 atctggaaat acaaatcggc gtaatcaatc tgatgcgaaa gcagattgcc aaacacgcgt
     4201 tcgagcttgg catggtcgag atcgtaaggc gcaagcagaa aacggttcgc ggtctctaac
     4261 gacgttatgg cagtttcggt atgcatgcgg ttcacgactt ataaaaaagt ttgcttatgc
     4321 tacacccggc gcagtgtgcg atgcgttaac gccggcaggc ttttcctcaa gctcgcctga
     4381 tattgcgggt tgaccccggc catcaccacg ccggagccgc gcgcaagccg atccaggatc
     4441 acgccccaag gatctacgat catgctgttg ccgtgcgtct cgcgaccgct cacatgatag
     4501 ccaccctgcg ccggcgccaa aacataagcc tggttctcaa tggcgcgcgc ccggatcaga
     4561 gtttcccaat gcgcttttcc ggtagtttcg gtaaacgcgg cgggcacgag aatgatgtcg
     4621 acgttgccca tcgcccgata caattcggga aagcgcaggt cataacagac cgaaagtccc
     4681 acccgtccaa acggcgtttc cgcgaccact acttcattac ccggctcaat ggtcgcctgt
     4741 tcatggtaat gctcgttgcc gaggtccaga ccgaataaat gtattttatc gtatcgggcg
     4801 gccattttgc ctttatcgtc gtagagcagg cagctgttgc gcaccttggt tggcaccgag
     4861 gctgccagcg gcaccgaacc gccggccagc cagattttat gttttttg
//
LOCUS       DATIOW010000143         8238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1487608, whole genome shotgun sequence.
ACCESSION   DATIOW010000143 DATIOW010000000
VERSION     DATIOW010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8238
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487608"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2263)
                     /gene="mutS"
                     /locus_tag="VLL03_04750"
     CDS             complement(<1..2263)
                     /gene="mutS"
                     /locus_tag="VLL03_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HSS46706.1"
                     /translation="MNKPELETRNAKHTGHTPMMQQYLRIKSDYPHMLVFYRMGDFYE
                     LFYDDAERAARLLDITLPSRGESAGSPVKMAGVPYHSVEQYLARLIKLGESVAICEQI
                     GDPAQAKGPVERKVVRIITPGTLTDSGLLDEKRDNLLLAIASQPKMLGLAWLNLAAGR
                     LRVLEIPESQLASELERLQPSEILLPDGFSHPLLENCGPAVKRLAPWQFDFDTATRNL
                     CKQFGTRDLSGFGCDGLHASVASAGALLEYARTAQGTAITHVNAIQVEHRDDFVRMDA
                     STRRNLEISETIRGESAPTLLSLLDACVTGMGSRLLRHWLHHPLCNHETIKLRLQAVS
                     SLLGDAIYRNAHKTLRNCADIERITSRIALKSARPRDLSGLRDSLKQMPKLQEHIASL
                     SSPRLAELSIALYVPEVLAELLQRTLQTEPSAMLRDGGVIADGYDTELDELRAISNNC
                     GEFLIQLEARERSRTGLSTLKVEYNKVHGYYIEVSRVQAERVPDDYRRRQTMKNAERF
                     ITPELKAFEDKALSAAERALAREKQLYDALLGALAPYISTLQNVAAAVAELDVLATFA
                     ERAASLNYTLPEFSEEPGILIEDGRHPVVEKQVEHFIANSTKLASTRQMLLITGPNMG
                     GKSTYMRQAALIVLLAHCGSFVPAKSAVIAPIDQIFTRIGSSDDLAGGRSTFMVEMTE
                     AANILHHATEHSLVLVDEIGRGTSTFDGLALAWAIAAHLLEKNRSFTLFATHYFELTR
                     LAQHYPQVANVHLD"
     gene            complement(2278..3066)
                     /locus_tag="VLL03_04755"
     CDS             complement(2278..3066)
                     /locus_tag="VLL03_04755"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HSS46707.1"
                     /translation="MHPMLTIAVRAARRAGHIINRASLNLDVLTVKHKTFNDLVSEVD
                     RAAEQAIIDVLKNSYPEHAILAEESGPQGDSDHLWIIDPLDGTTNFLHGYPQYAVSIA
                     FMHKKVLTQAVVYDPGRNELFTASRGRGAYLNDRRLRVSKRIKLADSLIGTGFPFREL
                     RHLETYLAMFRDLMKNTAGVRRPGAASIDLAYVAAGRLDGFWEIGLSPWDIAAGSLLI
                     QESGGLIGDFEGNDGYLESGNVVAGNPKIFGQLIKILAPHLPSA"
     gene            3184..3903
                     /locus_tag="VLL03_04760"
     CDS             3184..3903
                     /locus_tag="VLL03_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HSS46708.1"
                     /translation="MNPTNPLDNVRIVLVHPTHPGNIGAAARAMKAMGLGSLYLVNPG
                     RFPDREADALASGALDLLGAARICASLDEALSGTVLAVALTARPREFSHAAHNPREAA
                     GILLEEAGRSPVAVVFGSEMSGLSNEEVIKCRMVVHIPANPQYSSLNLAAAVQVMCYE
                     LRLAAVENERPQQTELEPANFEEIEMFYHHLEEVMIARGFMNPEQPKRLMERLRRLFG
                     RAHLEKEEVSILRGILKSLQK"
     gene            3965..4717
                     /gene="cysE"
                     /locus_tag="VLL03_04765"
     CDS             3965..4717
                     /gene="cysE"
                     /locus_tag="VLL03_04765"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HSS46709.1"
                     /translation="MFEHIKEDIACVFDRDPAARTTWEIITCYPGFHALLFHKFAHGL
                     WNADLKWLGRLVSHFSRWVTGIEIHPGAKIGRRVFIDHGMGVVIGETSEIGDDCTLYH
                     GVTLGGTSWNKGKRHPTLGKGVVAGAGAKILGPILIGDGARIGSNAVVVKEVPPGATV
                     VGIPGRVVDGEQEKTRAEVAEKMGFSAYGLSRDENDPVAKAIHGLLDHTVTIDQRIEC
                     IVKELEKLGAKVEDERATADKFDPNYLNKIVD"
     gene            4784..5254
                     /gene="iscR"
                     /locus_tag="VLL03_04770"
     CDS             4784..5254
                     /gene="iscR"
                     /locus_tag="VLL03_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HSS46710.1"
                     /translation="MRLTTKGRFAVTAMIDLAMRHGGGPVTLSEISERQKISLSYLEQ
                     LFGKLRRHSLVDSVRGPGGGYCLAKKIDEVSIADIILAVDEPIDATQCGGKENCHDDH
                     KCITHDLWASLNDRIFDYLQSVKLSQLVVSQKTGNVAVMRDQRKHKKSEPAVSA"
     gene            5261..6409
                     /locus_tag="VLL03_04775"
     CDS             5261..6409
                     /locus_tag="VLL03_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HSS46711.1"
                     /translation="MNLPVYFDHNATTLVDTRVLEAMHPYFTTHYGNASSRHEYGTQA
                     RKAINRAREQVAAAVKVEPSQVFFVSGGTEANNTFIKGAAAQLKPTQIAVSSIEHPCV
                     AKPAQELARQGWKLHKFAVDGEGRVDLADVEAALAQPTGIVSVMLANNETGVIQDVAA
                     VAEKARKQKTWMHTDAVQAFGKIAVDFSALNVQAMTISAHKIYGPKGAAALILDKRIE
                     LKPLLTGGGHEKGLRSGTENVPAIVGFGAACELAQSRLQNLAVQSGAQRERLEQGLRR
                     MNALLFGANAARLSNTSYFAFPGIDGETLVIELDKAGFAASSGSACSSVGHEPSPTLI
                     AMGVAPELAKSAVRVSLGKDNTIAQVEEFLQALQAVLMKLKRLAAVAV"
     gene            6443..6766
                     /locus_tag="VLL03_04780"
     CDS             6443..6766
                     /locus_tag="VLL03_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HSS46712.1"
                     /translation="MAITLTENAATHIKKVLAKRGSGMALRLGVKKVGCSGLAYTFDY
                     ADAVGARDHLFESHDAKVVVDAESLPYLDGSVLDFVKDGFKEVFKFDNPNIKDQCGCG
                     ESFNV"
     gene            6909..>8238
                     /gene="sufB"
                     /locus_tag="VLL03_04785"
     CDS             6909..>8238
                     /gene="sufB"
                     /locus_tag="VLL03_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HSS46713.1"
                     /translation="MSANLETLVNQPYKHGFVTSIEADVIPKGLSEEVIRMISKKKNE
                     PEWMLEFRLDAYRHWLTMREPHWATVRYPKLDYQDVIYYSAPKPKKQLASMDEVDPEL
                     RRTFEKLGVPLHEQKALAGVAVDIVFDSVSVTTTYKKKLAEIGIVFCSFSEAVQEHPG
                     LVRKYLGSVVPPTDNFFASLNSAVFTDGSFCFIPKGTKCPMDLSTYFRINTQDSGQFE
                     RTLIIAEEGASVSYLEGCTAPKFDTNQLHAAVVELIALDNAEIKYSTVQNWYAGDENG
                     KGGIYNFVTKRGLCKGVNSKISWTQVETGSAITWKYPSCVLLGDHSIGEFYSVALTNH
                     LQQADTGTKMTHIGKNTRSRIISKGIAAGRSSNSYRGQVKVGPRAHHARNYSQCDSML
                     IGDRCGANTFPYIDVRNPSAKVEHEASTSKIGEDQLFYFAQRGIGAEEAVS"
BASE COUNT         1806 a         2216 c         2508 g         1708 t
ORIGIN      
        1 catcgagatg cacattggcg acctgcgggt aatgctgtgc aaggcgagtg agctcgaaat
       61 agtgcgtggc gaaaagagta aaagagcggt ttttttccag caggtgcgcc gcaatggccc
      121 aagcgagcgc caagccatcg aaggtggaag tgccgcggcc gatttcatcc accagcacca
      181 ggctgtgctc ggtcgcatga tggagaatat tcgccgcttc ggtcatttcc accatgaagg
      241 tggagcgccc gccggcaaga tcgtcagaac tacctatgcg cgtgaagatt tgatcgatcg
      301 gagcaatgac cgcgcttttc gccggcacga acgagccgca atgagcaagc agcacaatca
      361 aagcggcctg gcgcatgtaa gtggatttgc cgcccatgtt ggggccggta atgagcagca
      421 tctgccgtgt gcttgcaagc ttggtgctgt tggcgatgaa gtgctcaacc tgcttttcca
      481 ccaccggatg gcggccgtcc tcgatgagaa ttccgggctc ttcgctgaat tcaggcaggg
      541 tgtagttgag gcttgccgcg cgttcggcaa aggtggccag tacatccaat tcagcgaccg
      601 cggcggccac gttttgcaaa gtggaaatgt aaggagcaag cgcgcccagc aatgcgtcgt
      661 aaagctgctt ttcccgcgcc aatgcccgct cggcggcgga aagcgccttg tcctcgaatg
      721 ccttgagttc cggcgtgata aagcgctcgg cgtttttcat ggtttggcgg cggcggtaat
      781 cgtcgggcac gcgctccgcc tgcacgcgcg aaacttcgat gtaatagccg tgcaccttgt
      841 tgtattccac tttcagcgtg ctcaagccgg tgcggctccg ctcgcgcgct tcaagctgta
      901 tcaggaattc gccgcagttg tttgaaatgg cgcgcagttc atcaagctcg gtgtcatagc
      961 cgtcggcaat tacgccgccg tcccgcagca ttgccgaggg ttcggtttgc agcgtgcgct
     1021 gcaaaagttc cgccagcact tcaggcacgt aaagcgctat cgaaagctca gcaaggcgcg
     1081 ggctggatag agaagcaata tgctcttgca gtttcggcat ctgcttcaga ctgtcgcgca
     1141 ggccggaaag gtcgcgcgga cgggcgcttt tgagcgcaat gcgcgaagta atgcgctcga
     1201 tgtcggcgca attccttaat gttttgtgag cgttgcgata gatggcgtca ccgagcagtg
     1261 aggacacggc ctgcaaacgc aatttgatgg tttcatgatt gcacagcgga tggtgcagcc
     1321 agtggcgcag caggcggctg cccattccgg tgacgcaggc gtcaagcagc gaaagcagcg
     1381 ttggcgcgga ttcgccgcga atggtctcgg aaatttccag attgcggcgg gtcgaggcat
     1441 ccatgcgcac gaaatcatcg cggtgttcga cttggatggc gttgacatgc gtaatagccg
     1501 tgccttgggc ggttctcgcg tattcaagca gcgcgccggc ggaggcaacg ctagcgtgca
     1561 gtccgtcaca gccgaaaccg ctcaaatcgc gagtgccaaa ttgcttgcaa agattgcgag
     1621 tagccgtgtc gaaatcgaac tgccacggcg cgagtcgctt cacggcaggg ccgcaatttt
     1681 caagcagcgg atgcgaaaaa ccatcgggca gcaggatctc ggaaggctgc aggcgttcca
     1741 gttcggaggc gagctggctc tcgggaattt ccagtacgcg caagcggccc gcggcgagat
     1801 taagccacgc aagtcccaac atctttggtt gtgaagcaat ggccagcaaa agattgtcgc
     1861 gcttttcgtc gagcaggccc gaatcggtga gcgttcccgg tgtgatgatg cgcacgactt
     1921 tgcgttcgac cggcccctta gcttgcgccg gatcgccgat ctgctcgcag attgccaccg
     1981 attcgcccag cttgatgagc ctggcgagat attgctccac cgaatgatag ggtacgcccg
     2041 ccatcttcac tgggctcccc gccgattcgc cgcggctggg gagcgtgatg tcgagaagcc
     2101 gtgccgctct ttccgcgtcg tcataaaata gttcataaaa atcccccatg cggtagaaca
     2161 ccaacatgtg cggatagtcc gacttgatgc gcaggtactg ctgcatcatc ggcgtgtgcc
     2221 cagtgtgttt cgcgttccgg gtttcgagtt ctggcttgtt catttctgaa cggcggttta
     2281 agccgaaggc aaatgcggcg cgaggatttt gataagctgc ccgaagattt tcggattgcc
     2341 ggcgaccacg ttgccgcttt cgagataacc gtcgttgccc tcgaagtcgc cgatcagccc
     2401 gccggattcc tgaatgagca ggctccccgc cgcgatgtcc cacgggctca aaccgatctc
     2461 ccagaagccg tccaatcgcc ccgctgcgac ataggcgagg tcgatcgaag ccgcgccggg
     2521 gcggcgcacg ccggcggtat ttttcatcag gtcgcggaac atcgcgagat aagtctcgag
     2581 atgtcttaac tcgcggaatg gaaaaccggt gccgatcagc gaatcggcga gcttgatgcg
     2641 cttgctcacg cgcagccgcc ggtcgttgag atacgcgccg cgcccgcggc tggcggtgaa
     2701 caactcgttg cgtccggggt cgtacaccac ggcttgagtc agcacttttt tgtgcatgaa
     2761 ggcgatggac accgcgtatt gtggatagcc gtgtaggaaa ttggtggtgc cgtcgagcgg
     2821 atcgataatc cacaggtggt cggagtcgcc ctgaggaccg ctttcttctg ctagaatcgc
     2881 gtgctctgga taagagtttt tcaacacatc gataatcgcc tgttccgccg cgcggtccac
     2941 ttcgctcacg agatcgttga aagttttgtg cttgacggtc agcacgtcaa ggttgagcga
     3001 tgcgcgattg atgatgtggc cagcgcggcg cgcggccctc accgcgatag tgagcatggg
     3061 gtgcatgatg gactttttaa ataaattttt accgcggagc gcggagacgc tgagaagtcc
     3121 ttaataatct ctgcgcctca gcgtctctgc ggttagcatt ttctggattg tcagtatttt
     3181 aatatgaatc ccacgaaccc gctggataac gtccgcatcg tgctggtgca tccgacccat
     3241 cccggcaaca tcggtgccgc tgcgcgcgcc atgaaggcca tgggcctggg ttcgctttac
     3301 ctcgtcaatc ccgggcgctt ccccgaccgt gaagcggacg cgctggcttc aggcgcgctg
     3361 gatctgctgg gcgcagcgcg tatttgcgcg tctctcgacg aggcgctttc cggtaccgtg
     3421 ctcgcggtgg cgcttaccgc gcggccaaga gagttttcac acgcggcaca taacccaaga
     3481 gaagccgcag ggatcctgct tgaagaggcc gggcgctcgc cggtcgcagt ggtgttcggc
     3541 agcgaaatgt cgggcttgag caacgaggag gtcatcaaat gccggatggt ggtgcatatt
     3601 ccggcgaatc ctcaatattc gtcgctcaat cttgccgcag cggtgcaggt gatgtgctat
     3661 gaattgcgtc tcgcggcagt ggaaaatgag cgtccgcagc aaacggaact cgaaccggca
     3721 aactttgagg aaatcgagat gttttatcat catctcgagg aggtcatgat agccagagga
     3781 ttcatgaatc ccgaacagcc gaagcggctg atggagaggc tgcgccgcct gtttggccga
     3841 gcgcacctgg aaaaagaaga agtcagcatc ctcaggggga ttttgaagtc gctgcaaaaa
     3901 tagttgactg atttagtagg atatagcaaa cttatgcttc ttaaggcaaa ttgggctgag
     3961 taaaatgttc gaacatatca aggaagacat tgcctgcgta ttcgaccgcg accctgcggc
     4021 gcgcaccacc tgggaaatca tcacctgcta tcccggcttt catgccctgc tgtttcacaa
     4081 attcgcgcat gggttgtgga acgccgatct caaatggctg gggcggctgg tttcgcattt
     4141 ttcgcgctgg gtgaccggca tcgaaatcca tccgggagcg aaaatcggcc gccgggtgtt
     4201 catcgatcac ggcatgggtg tggtgatcgg cgagacttcg gaaatcggcg acgactgcac
     4261 tctctaccac ggcgtgactc tgggcggcac ctcgtggaac aagggcaagc gccatcccac
     4321 tcttggcaag ggggtggtgg cgggcgcggg cgcgaaaatc ctcgggccga ttttgatcgg
     4381 ggacggcgcg agaatcggct cgaacgcagt ggtggtgaaa gaagttccgc ccggcgcgac
     4441 cgtggtcggc attcccgggc gtgttgtcga cggcgagcag gaaaagacgc gtgccgaggt
     4501 ggcggaaaaa atgggtttct ccgcttatgg tttaagccgg gacgaaaacg atccggtggc
     4561 gaaagccatc cacggtttgc tggatcatac tgtcaccatc gaccagcgca tcgaatgcat
     4621 tgtcaaggaa ttggaaaagc tgggcgcgaa agtcgaggac gaacgcgcca ccgccgacaa
     4681 atttgacccg aattacctga acaaaatagt agactgaaac gttcgggtat tgtatagttg
     4741 acaaatttaa tcaagtattt ggcatgccac ggaaggtgag cctatgcgat tgacaaccaa
     4801 gggacgtttc gcggtcacgg cgatgatcga tctggcgatg cgccacggcg gcgggccggt
     4861 gacgctctcc gaaatcagcg agcgccagaa aatttctctc tcgtatcttg agcagctgtt
     4921 cggaaaacta cgccgccact cgctggtgga cagcgtgcgc ggccccggcg gtggctattg
     4981 cctcgccaaa aaaatagacg aagtgtcgat cgcggacatc attctggcgg tggacgagcc
     5041 gatcgacgcc acccaatgcg gcggcaaaga aaactgccac gacgaccaca agtgcatcac
     5101 ccacgattta tgggcttcgc tcaatgaccg catcttcgat tatctgcaat cggtgaagct
     5161 ctcgcagttg gtggtgagcc agaaaaccgg caatgttgcg gtgatgcgcg accagcgcaa
     5221 gcacaagaaa agcgagcctg cggtctccgc gtaaaacggc atgaatctcc cggtttattt
     5281 tgatcacaac gccaccacgc tggtggatac ccgcgtgctg gaagcgatgc atccgtactt
     5341 caccacgcat tacggcaacg cctccagccg ccacgaatac ggcacgcagg cgcgtaaagc
     5401 catcaatcgt gcccgcgagc aagtagcggc tgccgtcaag gtggagcctt cgcaagtttt
     5461 ttttgtaagc ggcgggacgg aagcaaacaa caccttcatc aagggcgcgg cggcacagtt
     5521 aaagccgacg caaatcgcgg tgagcagcat cgaacatccc tgtgtggcca agccggcgca
     5581 ggaacttgcc cgtcaaggct ggaagctgca caaattcgcc gtggatggcg aggggagggt
     5641 tgatcttgcc gatgtggaag ccgcgctcgc acagccgacc ggcatcgtgt cggtgatgct
     5701 tgccaacaac gaaaccggcg tgattcagga cgtggcggcg gtggcggaaa aagcgagaaa
     5761 acagaaaacc tggatgcaca ccgacgcggt gcaggctttc ggcaagattg cggtggattt
     5821 cagcgcgctt aacgtgcagg cgatgacgat ttcggcgcac aagatttacg gccccaaggg
     5881 cgcggcggcg ctgattctag ataaacgcat tgaattgaaa ccgctgctga cgggcggcgg
     5941 gcatgaaaag ggtttgcgct ctggcaccga gaacgtgccg gcgatagtcg gtttcggcgc
     6001 ggcctgcgag cttgcgcaaa gccgtttgca aaatctcgcg gtgcagtcgg gcgcgcagcg
     6061 cgagagattg gaacagggcc tgcgcagaat gaatgcgttg ctgtttggcg ccaatgctgc
     6121 gcggctttcc aacaccagct atttcgcttt tcccggcatt gacggcgaga cgctggtcat
     6181 cgagctcgac aaggcgggct tcgcggcttc cagcggctcg gcatgctcca gcgtcggcca
     6241 tgagcccagc ccgacactga ttgcaatggg cgtggcaccc gagctggcga agagcgcggt
     6301 gcgtgtaagc ctgggcaagg acaacacaat agcgcaggtt gaggaatttt tgcaggcgct
     6361 gcaggcggtg ctcatgaagc tgaaacgtct ggcggcggtt gcggtctgac acttgaatac
     6421 gaatgaagga gtaatggcaa agatggcaat cactctgact gagaatgcgg caacccatat
     6481 taaaaaagtt ctcgccaagc gcggcagcgg catggcgttg cgcctgggcg tgaaaaaagt
     6541 cggttgttcg ggcctggctt acacctttga ttacgccgac gcggtgggag cgcgcgacca
     6601 tctgttcgag tcgcacgacg ccaaagtggt ggtggatgcg gagagcctgc cttatctgga
     6661 cggttcggtg ctggacttcg tcaaggacgg cttcaaggaa gtgttcaagt tcgacaatcc
     6721 caacatcaaa gaccagtgcg gatgcgggga aagttttaat gtttaacgca tccttcgctt
     6781 aattcggttc tgcaatttac cagcccggga aaatgaatag ttgactgaat ttgtcgggca
     6841 tattaaactg ctaatagttg agtaaataac tcgggtatag aaagctttat aatcatggct
     6901 taatgtaaat gagtgctaat ttagaaacac tggttaacca gccttataaa cacggcttcg
     6961 tcacctcgat tgaggcggat gtcatcccca aggggttgag cgaagaagtg atccggatga
     7021 tttcgaagaa aaagaacgaa ccggagtgga tgctggaatt caggctcgat gcctaccgcc
     7081 actggcttac catgcgggaa ccgcactggg ccactgtgcg ctatccgaaa ctcgattatc
     7141 aggacgttat ttattactcc gcgcccaagc cgaagaagca gcttgcgagc atggatgaag
     7201 tggatcccga attgcggcgc acctttgaga aactgggcgt gcccttgcac gaacagaagg
     7261 cgctcgctgg cgtggcggta gacatagtgt tcgatagcgt ctcagtgacc acgacttata
     7321 agaagaagct ggccgaaatc ggcattgttt tctgctcctt ctccgaagcg gtgcaggaac
     7381 atcccgggct ggtgaggaaa tatctgggca gcgtggtgcc gcccaccgac aatttcttcg
     7441 cttcgctcaa ttccgccgta ttcaccgacg gctcgttctg tttcatcccc aagggtacca
     7501 agtgcccgat ggatttgagc acttatttcc gcattaacac ccaggattcc gggcagtttg
     7561 agcgcacgct gattatcgcc gaagagggcg cctcggtgtc ttatctggaa ggctgtaccg
     7621 cgccgaaatt cgacaccaac cagctgcacg ccgcagtggt ggaattaatc gcgctcgaca
     7681 acgctgaaat caaatactct accgtgcaga actggtacgc cggcgatgaa aacggcaagg
     7741 gcggcattta caattttgtg accaagcggg gcctgtgcaa gggcgtaaat tcgaaaattt
     7801 cgtggacgca ggtggaaacc ggctcggcga ttacctggaa atatccgtcg tgcgttttgc
     7861 tgggcgacca ttccatcggc gagttttatt cggttgcgct caccaatcat ctacagcagg
     7921 ctgacaccgg gaccaagatg acgcacatcg gcaaaaacac ccgcagccgc atcatcagca
     7981 agggcattgc cgcgggacgc tccagcaaca gctaccgcgg ccaggtgaag gtcggccccc
     8041 gcgcgcatca cgcgcgcaat tattcgcagt gcgattcgat gttgatcggc gaccgctgcg
     8101 gcgccaatac ctttccgtat atcgacgtgc gcaatccaag cgccaaggtg gagcacgagg
     8161 cgtcgacttc caaaattggc gaagaccagt tgttctactt cgcgcagcgc ggcataggtg
     8221 ccgaggaagc ggtgtcga
//
LOCUS       DATIOW010000144         7993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1995066, whole genome shotgun sequence.
ACCESSION   DATIOW010000144 DATIOW010000000
VERSION     DATIOW010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7993
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1995066"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..506)
                     /locus_tag="VLL03_04790"
     CDS             complement(132..506)
                     /locus_tag="VLL03_04790"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HSS46714.1"
                     /translation="MTDSAIVLFAHGARDAQWAEPFRKIKAMVRARKPGMPVELAFLE
                     IMQPSLDQAIAALVAKNIKTIGIIPLFMAQGGHLKEDLPRRLSEIKKLNPAISFRISP
                     PIGEVEGILTAIADWICKEHDT"
     gene            complement(509..958)
                     /gene="dut"
                     /locus_tag="VLL03_04795"
     CDS             complement(509..958)
                     /gene="dut"
                     /locus_tag="VLL03_04795"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HSS46715.1"
                     /translation="MKKLDVKILDPRIADQLPHYATPGSAGLDLRACITQSITLKPGQ
                     AELIPSGIAIHLDDPGLAAMVLPRSGLGHKHGIVLGNLVGLIDSDYQGQIFVSVWNRG
                     QRDFVLNPMERIAQLVVVPVVQVAFNVVREFNESHRGEDGFGSTGKH"
     gene            complement(955..2184)
                     /gene="coaBC"
                     /locus_tag="VLL03_04800"
     CDS             complement(955..2184)
                     /gene="coaBC"
                     /locus_tag="VLL03_04800"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HSS46716.1"
                     /translation="MSQPGNKRLLLGITGGIAAYKAAELTRLLVQDGIEVRVVMTEAA
                     CRFVGPATFQALSGNTVYTDLWDARIANNMAHIDLSREADMVLVAPASADFIAKLVHG
                     RADDLLSTLCLARECPLYVAPAMNRQMWENPATQRNIAILEKDGVTILGPGSGDQACG
                     ETGMGRMLEPETLLEEIKAGFQAKLLQGKRVLITAGPTFEAIDAVRGITNSSSGKMGY
                     AVARAALEAGAEVALVSGPVSLAAPARARLIPVTNAQEMFAAVKESVGTADIFIGVAA
                     VADYHVVNPSKQKIKKSGQSLNLQLAPNPDILEYVAGLPNAPFCVGFAAESENLQAHA
                     EQKRRDKKIPLLAANLVQTALGSDESELVLFDDAGAHPLPRAAKTTLARQLIKHIAQL
                     YAKSRTVTAFKKNQAGK"
     gene            2263..2937
                     /gene="radC"
                     /locus_tag="VLL03_04805"
     CDS             2263..2937
                     /gene="radC"
                     /locus_tag="VLL03_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HSS46717.1"
                     /translation="MAINDWPRDERPREKLLAKGAQSLSEAELLAIFLRTGVAGKSAV
                     DLARDLIKRFGSLTRLFNATEADFCAAPGLGPAKYTQLQAVLEMAKRALNEEIKSGDA
                     LTSPKAVRDYLRLALAGREYEVFAAIFLDAQNRVLVVEELFRGTLSKTSVFPREVVKR
                     ALHHNAAAVIFAHNHPSGTAEPSGADEALTAALKKSLGLVDVKVLDHFIVGSGSIMSF
                     AERGLI"
     gene            3065..3292
                     /gene="rpmB"
                     /locus_tag="VLL03_04810"
     CDS             3065..3292
                     /gene="rpmB"
                     /locus_tag="VLL03_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HSS46718.1"
                     /translation="MARVCEITGKTPMLGNRVSHANNKTRRRFLPNLHYRKFWVESEK
                     RWVSLRLTNAALRTIDRKGIEVVLAELRGKK"
     gene            3304..3459
                     /gene="rpmG"
                     /locus_tag="VLL03_04815"
     CDS             3304..3459
                     /gene="rpmG"
                     /locus_tag="VLL03_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HSS46719.1"
                     /translation="MREKIKLESSAGTGHFYTTTKNKRNQTDKLELKKFDPVARKHVM
                     YKETKLK"
     gene            complement(3578..4759)
                     /locus_tag="VLL03_04820"
     CDS             complement(3578..4759)
                     /locus_tag="VLL03_04820"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HSS46720.1"
                     /translation="MTYFSGLIDLPWWGYVLVALVFTHITIAAVTIYLHRCQAHCALD
                     LHPVVSHFFRFWLWLTTGMVTKEWTAIHRKHHAKVETAEDPHSPQVCGLRKVLWEGTE
                     LYKREAKNPETLEKYGHGTPNDWLEHNLYGKYSILGISIMAVIDFALFGFVGVTIWAV
                     QMMWIPFFAAGVINGVGHFWGYRNFACADASKNIVPWGILIGGEELHNNHHAYVTSAR
                     LSNKWYEFDIGWLYIRIMEIFGFADVKKIAPKIRFNFAKAQCDYETLQAVITHRYDVL
                     AKFAKSVKQTYAEEIRKLKERAAQIDGTTWRNLKKRWLHLDAKDLKAQERRQLNEVLS
                     KSKVMRTVYSMRQELSQLWQRSTASKDQLLKQLQDWCLRAEESGIVSLQKFSRKLRGY
                     I"
     gene            complement(4923..5504)
                     /locus_tag="VLL03_04825"
     CDS             complement(4923..5504)
                     /locus_tag="VLL03_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HSS46721.1"
                     /translation="MTKLISLKTGLALMLFAAALSAIAFQEPFLSSGKSIAATGKIEL
                     AFTPEDAADLMVIRAIRGAHKQILVQAFSFTHRDIAHALIQAKKRGVDVQLIADAEQT
                     DKIQPNMIADIAASGIRTYLDFQHQSAHNKIMVIDASSPDSAVVTGSFNFTYAAQFKN
                     AENLVIFRGNPEITAAYLENWKRHLAHASRYRR"
     gene            complement(5497..6756)
                     /locus_tag="VLL03_04830"
     CDS             complement(5497..6756)
                     /locus_tag="VLL03_04830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HSS46722.1"
                     /translation="MSLAAARLDMAVAAWRLLSPLSGPADNLLSRFFRDHPKLGVHDR
                     GFIADTLFGLLRHRFYAEHLAENQTPRRLVLAYLTRFAGLSLRELEPCLSRDEAKWLA
                     KIKEKAGEDLPFHVRAELPEWLIEKLKVSLPENEILALGRSMQNPAPLDLRVNTMRAS
                     REEVLHSLQVEGLKAEPAPYSPIGIRLQNKISLNRHPLFLSGKVEVQDEGSQLLCFLL
                     APRRHEMVVDFCAGSGGKALALGAMMHNHGRVYAFDVSDKRLNHLKPRLKRSGLSNIH
                     PQRIDSENDQKIKRLAGKIDRVLVDTPCSGLGTLRRNPDLKWRQSPQSIEELKQKQAA
                     ILKRAATLLKPGGRLVYATCSILPEENEAIVTDFLHENRHFIIMNGSAVLAQQAIPLA
                     TGETLRLFPHLHGTDGFFAAVMEKTND"
     gene            complement(6757..7446)
                     /locus_tag="VLL03_04835"
     CDS             complement(6757..7446)
                     /locus_tag="VLL03_04835"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HSS46723.1"
                     /translation="MRRFILLLVCLSLNAAAAELPRRVTITYAVSRGILGLGTGTDVL
                     ETNGKNYSIVSQTHPSGLAALIPKAKIRRESRGTITAQGLRPQQFLDKRGNEQPAIAK
                     FDWGKKLITFEREGKVESAELPKTTHDRLSIAYNFAFSQPQGKELQFSMTDGKQLSNY
                     RYVLLGEEKLNTPLGELQTLHWQKQREKETDRGADLWLAPDHFMLPVRIVFTDKDGSR
                     FEQIATQISYQ"
     gene            complement(7446..>7993)
                     /gene="purN"
                     /locus_tag="VLL03_04840"
     CDS             complement(7446..>7993)
                     /gene="purN"
                     /locus_tag="VLL03_04840"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HSS46724.1"
                     /translation="SSNPAAGGLESARKHGIATLTLDHRDYAGREDFDAELASRIKTF
                     KPDLIVLAGFMRILGDIFVSRFENRIMNIHPSLLPAFPGLDTHARALAAGVKIHGCTV
                     HFVTPQLDHGPIIIQAAVPVLPGDTEQVLAARVLEQEHRIYPQAVRWFAEGKLVIENQ
                     RVRVIDASDKISPMRVPEAEN"
BASE COUNT         1654 a         2174 c         2382 g         1783 t
ORIGIN      
        1 gtctctggct tcgggggtgt tcgccacctc ggcgatcatg ggatggccgg cgatgcttaa
       61 tggaacgagt tccgtatcag cggcataagc cgccaaggac agcaggaaag cagttgcggc
      121 gaaaaaacgc ttcatgtgtc gtgctccttg caaatccagt cggcaatcgc ggttaagatt
      181 ccttccactt cgcctatcgg cggcgaaatt ctgaaagaaa tcgctgggtt aagttttttt
      241 atctcggaaa gccggcgcgg caggtcctct ttcagatggc cgccttgcgc catgaacagg
      301 ggaatgatgc cgatagtttt gatatttttc gcgaccagtg cggcgatggc ctgatccagc
      361 gaaggctgca tgatttccag aaaagcgagt tccaccggca tgccaggttt tctggcgcgc
      421 accattgcct ttattttgcg gaacggctcc gcccattgcg catcgcgcgc gccgtgcgcg
      481 aacagtacga ttgccgaatc agtcattgtc aatgcttgcc ggtacttcca aatccgtctt
      541 ctccgcgatg gctctcattg aattcccgca ctacgttgaa ggcgacttgc accacgggca
      601 cgaccaccag ttgcgcgata cgctccatgg gattgagcac aaaatccctc tgcccgcggt
      661 tccacaccga aacgaatatc tgtccctggt aatcggaatc gatcaatcca accagattgc
      721 cgagcacgat gccgtgcttg tgcccgagtc ccgagcgcgg cagcaccatg gcggcgaggc
      781 cggggtcatc aagatgaatg gcaatgccgg agggaatcag ctcggcctga ccgggtttga
      841 gtgtaatgct ttgagtgatg caggcgcgca aatccagacc cgccgaaccg ggagtggcgt
      901 agtgaggaag ctggtctgcg atgcgcgggt cgaggatttt aacgtcgagt tttttcattt
      961 gcccgcttgg tttttcttga acgccgtgac cgtgcgggat ttggcgtaaa gctgtgcgat
     1021 gtgcttgatc aactgccggg cgagcgtggt ttttgctgcg cgcggcaggg ggtgcgcgcc
     1081 cgcatcgtcg aataaaacca gttcgctttc gtcactgccc agcgcggttt gcaccagatt
     1141 ggcggcaagc agcgggattt tcttgtcgcg gcgtttttgt tcggcatgtg cctgcaggtt
     1201 ttcgctttcc gcggcaaaac cgacgcagaa gggcgcattg ggcaggccgg cgacatactc
     1261 cagaatatcc ggattgggcg caagctggag attgaggctc tggccgcttt tcttgatttt
     1321 ttgtttgctc ggatttacca cgtgatagtc ggcgaccgcc gccaccccta taaatatgtc
     1381 agccgtcccg actgattcct tgaccgccgc aaacatttct tgcgcgttgg tgaccggaat
     1441 gagcctggcg cgggcaggag cggcaaggct caccgggccg cttaccaagg cgacttccgc
     1501 tcccgcttcg agcgcggcgc gggcgaccgc gtagcccatt ttgccggagc tggaattggt
     1561 gatgccgcgc accgcatcaa tcgcttcaaa tgtcgggccg gcggtgatca gcacgcgctt
     1621 gccttgaagc agcttcgctt gaaagcccgc tttgatttct tccaagagcg tctcgggttc
     1681 gagcatgcgc cccatgccgg tctcgccgca ggcctggtcg ccgctgccgg gtccgagtat
     1741 cgtcacgccg tctttttcaa gtatggcgat gttgcgttgg gtggcggggt tttcccacat
     1801 ctgccggttc atcgccggtg cgacatagag cggacattcg cgcgcgagac agagcgtgga
     1861 gagcaaatca tcggcgcgcc cgtgtaccag cttggcgatg aagtcggcgc tagccggcgc
     1921 aaccaacacc atgtcggctt cgcggctcaa atcgatatgc gccatgttgt tggcaatgcg
     1981 cgcatcccac aggtcggtgt agaccgtgtt gccggagagc gcctggaacg ttgcggggcc
     2041 gacaaagcga caggccgctt cggtcatcac cacccgcact tcgatgccgt cctgtaccaa
     2101 aaggcgcgtc aattccgcgg ctttgtaggc ggcgatgcct ccagtgatgc cgagcagaag
     2161 gcgtttatta cccggttgac tcatgacgac cactctaaag tgttgacccg acctagtgta
     2221 atatattgat gcggataaag aaactgtcaa ctaaaaccac gtatggcaat caacgattgg
     2281 ccgcgggacg aacgccccag agagaagctg ctggccaaag gcgcgcagag cctttcggag
     2341 gccgagcttt tggcgatttt tctgcgcacg ggagtggcgg ggaaaagcgc ggtagacttg
     2401 gcgcgcgatt taatcaagcg cttcggcagt ctcacgcggc ttttcaacgc tactgaagcg
     2461 gatttttgcg cagcgccggg cttgggcccc gccaaataca cgcagttgca agccgtgctg
     2521 gaaatggcga aacgggcgct caacgaggaa atcaaaagcg gcgatgcgct gacttccccc
     2581 aaggcagtgc gagattattt gcgcctggcg cttgccggtc gcgaatacga agtgttcgcg
     2641 gcgatatttc tcgacgcgca aaaccgcgtg ctggtagtag aagagctctt ccgcggcacg
     2701 ctgtctaaga ccagtgtttt tccgcgggaa gtggtgaagc gcgcactgca tcacaacgcc
     2761 gcggcggtga ttttcgccca caaccaccct tccggcaccg ccgaacccag cggcgccgac
     2821 gaggcgctga ccgccgcgct caagaaatct ctcgggctgg tggacgtcaa agtgctggat
     2881 cactttatcg tcggcagtgg ctcaatcatg tcgttcgccg agcgcgggtt gatttagctc
     2941 tgtctagctt gtcgaaggat tggccgagcg ggggccgctt tagggcggcg ggaatatttt
     3001 ttccttgaga gttagccctt aaatgggtaa aatgcgcact tttcgagatt cgggataagc
     3061 cctcatggcc agagtatgcg aaatcaccgg caagacgccg atgcttggga accgcgtttc
     3121 tcacgccaac aacaagaccc gccgccgttt tctgcccaac ctgcactacc gcaaattttg
     3181 ggtggagagc gaaaagcgct gggtgagcct gcggctcacc aacgccgcct tgcgcaccat
     3241 cgacagaaaa ggcattgaag tagtgcttgc cgaactgcgc ggcaagaaat aaaggataga
     3301 atcatgcgcg agaaaatcaa gctcgaatcg tccgctggta ccggccactt ctacaccacc
     3361 accaagaaca aacgcaacca gacagataaa ttggaactca agaaattcga cccggtggcc
     3421 cgcaagcatg tgatgtacaa ggaaacgaaa ttgaaatgaa cgcgtggcgt tggtcctgag
     3481 tgccagcgaa ggatcttaaa aaaagcccgc gtcgagcggg ctttttattg gtggacgcga
     3541 cagatctaaa aaacggattc ttgctcagca tgacaggcta gatgtagccg cgcagcttgc
     3601 gcgagaactt ctgcagcgaa acaatgccgc tttcctcggc gcggaggcac cagtcttgca
     3661 gctgtttgag cagttgatct ttcgaagcgg tggagcgctg ccacagttgg gacaactcct
     3721 gacgcatcga gtaaacggtt ctcatgactt tgctcttgct taaaacttca ttgagctggc
     3781 ggcgttcctg cgctttcagg tctttggcat caaggtgcag ccagcgtttt ttcaggttgc
     3841 gccatgtcgt gccgtcaatt tgggcggcgc gttctttcag tttgcggatt tcttccgcgt
     3901 aggtctgctt caccgatttg gcgaatttag cgagcacatc gtagcggtgg gtaatcaccg
     3961 cctgcagcgt ttcgtagtcg cactgcgcct tggcgaaatt gaagcggatt ttcggtgcga
     4021 ttttcttgac gtcagcaaag ccgaaaattt ccatgatacg gatgtagagc cagccgatgt
     4081 cgaactcgta ccatttgttg gaaagccgcg ccgaggtgac gtaggcgtga tggttgttgt
     4141 gcagttcctc gccaccgatc agaatgcccc agggaacgat gttcttgctg gcgtcggcgc
     4201 aagcaaaatt gcggtagccc cagaagtggc cgacgccgtt gatgacgccg gcggcgaaaa
     4261 acggaatcca catcatctgt accgcccaga tggtgacacc gacgaatccg aacagggcaa
     4321 agtcaatcac tgccatgatg gaaatgccga gtatgctgta tttgccatac aggttgtgtt
     4381 ccagccaatc gttcggcgtg ccgtggccgt atttttccag cgtttcgggg ttcttggctt
     4441 ccctcttgta gagctcagtg ccttcccaca gcactttgcg caagccgcat acttgcgggc
     4501 tgtgcgggtc ttccgcggtt tccaccttgg cgtggtgctt gcggtggatc gctgtccatt
     4561 ccttggtcac catgccggtg gtgagccaca gccagaagcg gaagaaatgg ctgacgaccg
     4621 gatgcaagtc aagcgcgcaa tgagcctggc agcggtgcag gtaaatggtg acggcggcga
     4681 tggtgatatg ggtaaagacg agggcgacga ggacgtagcc ccaccacggc aagtcaatca
     4741 gtcccgagaa ataagtcatt aggtttaggc accacaaaat ataacggggc tattttacgc
     4801 gatatcgggg gtgaagcaaa gggcttttgt tgtgtgatat ttggcaaaaa cccttttctt
     4861 gtgaagtagt tggcaaatta agcttggtcg gcggcgacag ttcgccatag ggagacggtc
     4921 gattaccgac ggtagcgcga cgcgtgcgcc agatggcgct tccagttttc cagataagcc
     4981 gcagtgattt ccggattgcc gcggaatatc accagatttt ccgcgttctt aaattgtgcg
     5041 gcgtaggtga aattaaacga accggtgacg acggccgaat cggggctgct ggcgtcaatc
     5101 accatgattt tgttgtgcgc gctctggtgc tggaaatcga gataagtccg gatgccagaa
     5161 gcggcgatgt cggcaatcat gttcggctgg attttatcgg tttgttccgc gtcggcgatg
     5221 agctgcacgt ccactccgcg ttttttggct tggatcagcg catgggcgat atcccgatgg
     5281 gtgaaactga acgcctggac cagaatctgc ttgtgtgcgc ccctgatagc tctgatcacc
     5341 attaggtcgg ccgcgtcttc aggggtaaaa gccaactcga ttttaccggt ggcggcaatc
     5401 gacttgcctg atgataaaaa cggctcctga aacgcaattg cggaaagcgc ggcggcgaag
     5461 agcatcaacg ccaggccggt tttgagtgaa atcaatttag tcatttgttt tctccatcac
     5521 cgctgcaaaa aaaccgtctg tgccgtgaag atgcggaaac agccgcagag tttcgcccgt
     5581 agcgagcgga attgcctgct gcgccagaac cgcactgcca ttcatgatga taaagtgccg
     5641 attttcatgc aagaaatcgg tcacaatggc ttcattttcc tcgggcagga tgctgcaggt
     5701 cgcgtaaacc aatcggcctc ctggtttgag caaggttgcg gcgcgcttga ggattgccgc
     5761 ctgtttttgc ttgagttcct caatgctctg cggcgattgc cgccatttga gatcaggatt
     5821 gcggcgcagc gttcccaggc cgctgcacgg cgtatccacc agcacgcggt cgatttttcc
     5881 ggcaaggcgc tttatttttt ggtcattctc gctgtcaatg cgctgcggat gaatattgct
     5941 taaccctgag cgtttcaacc gcggtttcag atggttgagc cgcttgtcgg aaacatcaaa
     6001 agcgtaaacc cgcccgtggt tgtgcatcat ggcgcccagc gccagtgcct tgccgccgct
     6061 gccggcgcag aaatccacca ccatctcatg ccgccgtggc gcgagcagga agcacaacag
     6121 ctgactgcct tcgtcctgca cttccacctt gccggaaaga aacagcgggt ggcggttaag
     6181 cgaaattttg ttttgcaggc gaatgccgat cggcgaataa ggagcgggct ctgctttaag
     6241 accctcgact tgcaggctgt gcagcacttc ctcgcggctg gcgcgcatcg tgttgacccg
     6301 caaatccagc ggagcgggat tctgcatgct gcgcccgagc gcaagaattt cgttttccgg
     6361 aagcgagact ttgagttttt caatcagcca ttcgggcagt tcggcgcgga cgtgaaaggg
     6421 caaatcctcg ccggcttttt ccttgatttt ggccagccat ttcgcttcgt cgcgggaaag
     6481 acaaggttca agttcgcgca ggctcaagcc cgcaaaccgg gttagatagg caagcaccag
     6541 ccgtcttggt gtttggtttt cggccagatg ctccgcataa aagcggtgcc gcaggaggcc
     6601 gaacagcgta tcggcgatga agccgcggtc gtgtacgccg agcttgggat ggtcgcggaa
     6661 aaagcgcgag agcagattgt ccgccggccc gctcaaagga gagaggaggc gccaggcagc
     6721 aacagccatg tcgaggcgtg cagcggcaag agacatttat tgatagctga tttgcgttgc
     6781 gatctgctcg aaacggctgc catccttgtc ggtgaagacg atgcgcaccg gcagcatgaa
     6841 gtgatccggg gcgagccaca aatccgcgcc gcggtcggtt tccttttcgc gctgcttttg
     6901 ccagtgcagc gtctgcaatt ctcccaatgg cgtgttgagt ttttcctcgc ccaacagcac
     6961 atagcgataa ttggaaagtt gcttgccgtc ggtcatggaa aactgaagct ccttgccttg
     7021 cggctggctg aaggcaaagt tgtaggcgat gctcaggcgg tcatgcgtgg tcttgggcaa
     7081 ttccgcgctt tccactttgc cttcgcgctc gaaggtgatg agctttttcc cccagtcaaa
     7141 tttggcaatc gcgggctgct cgttgccgcg cttgtcgaga aactgctgcg ggcgcaggcc
     7201 ttgagccgtg atggtgccgc gcgactcgcg ccggattttg gccttgggta tcagcgcggc
     7261 aaggccgctt ggatgagtct ggctgacgat ggaataattc ttgccattgg tctccagcac
     7321 atctgttccc gtgcccagac ccaaaatgcc gcggctgact gcataggtga tggtgacgcg
     7381 ccgcggcagc tcggctgccg cggcattgag cgaaaggcaa accaatagca gaatgaaccg
     7441 cctcatcaat tctccgcttc gggcacccgc atgggcgaaa ttttgtcgct agcgtctata
     7501 acgcgcacgc gctggttttc gatgaccagc ttgccttccg caaaccagcg caccgcctgt
     7561 ggataaatgc ggtgctcctg ctccagcacc cgggcagcaa gcacctgttc agtatcgccg
     7621 ggcaacaccg gaaccgcggc ctggatgatg atggggccgt gatcgagttg aggggtgacg
     7681 aaatgcaccg tgcagccgtg gatttttacg ccggccgcga gcgcccgcgc gtgagtgtcg
     7741 agaccgggaa acgcgggcag caaagacgga tgaatattca tgatgcggtt ttcaaaacgg
     7801 ctgacaaaga tgtcgccgag aatgcgcatg aagccggcca acacgatcaa atccggcttg
     7861 aaggttttga tgcgcgacgc cagttccgca tcaaagtctt cgcggcctgc gtaatcacga
     7921 tgatcgagcg taagcgtggc gatcccatgc ttcctggcgc tctccagtcc cccggcagcg
     7981 ggattgctgc tga
//
LOCUS       DATIOW010000145         3785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1083252, whole genome shotgun sequence.
ACCESSION   DATIOW010000145 DATIOW010000000
VERSION     DATIOW010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083252"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VLL03_04845"
     CDS             complement(<1..264)
                     /locus_tag="VLL03_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HSS46725.1"
                     /translation="MSKGKFERTKPHVNVGTIGHVDHGKTTLTAAMTMVLAKKYGGEV
                     KSYDQIDSAPEEKARGITINTAHVEYETSKRHYAHVDCPGHADY"
     gene            complement(277..2388)
                     /gene="fusA"
                     /locus_tag="VLL03_04850"
     CDS             complement(277..2388)
                     /gene="fusA"
                     /locus_tag="VLL03_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HSS46726.1"
                     /translation="MARKTPIERYRNIGITAHIDAGKTTTTERILFYTGVSHKLGEVH
                     DGTTIMDWMEQERERGITITSAATTCFWKGMDHNHPEHRINIIDTPGHVDFTIEVERS
                     LRVLDGACTVLCAVGGVQPQTETVWRQANKYHVPRLAFVNKMDRQGANYMRVYDQVKS
                     RLKANPVLIQLPIGAEEKFEGVVDLVKMKAIYWDDSTQGMKFEMRDVPANMLGECKHW
                     REKMVETAAEASEELMHKYLEGVALTEEEIRKGLRQRTINNEIVPMLCGSAFKNKGVQ
                     AMLDAVIDYMPAPVDIPAIKGELENGQPGERHASDDEPFSGLAFKIATDPYVGQLIFF
                     RVYSGVVTSGDTIYNPIKGKKERIGRLLQMHANQRDEIKEVRAGDIAAAVGLREASTG
                     DTLCDPGKIITLERMVFPEPVISQAVEPKTKADQEKMGIALNRLAQEDPSFRVRTDEE
                     SGQTIISGMGELHLEIIVDRMKREFGVEASVGKPQVAYRETIKKMVDDVEGKFIKQSG
                     GRGQYGHVWLKVEPNPGKGYEFVDAIKGGAVPREFIPAVQKGLVETLPNGVLAGFPVV
                     DVKVTLFDGSYHEVDSNENAFKMAASFAFKDGMRKASPTLLEPMMAVEVETPGDFVGN
                     VIGDLSSRRGMVQGQEDVAGIMVIKAEVPLAEMFGYSTTLRSLTQGRATYTMEFKHYV
                     EAPKQVAEAIINKFTGKDVKK"
     gene            complement(2419..2889)
                     /gene="rpsG"
                     /locus_tag="VLL03_04855"
     CDS             complement(2419..2889)
                     /gene="rpsG"
                     /locus_tag="VLL03_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HSS46727.1"
                     /translation="MPRRREIPKRHVLPDPKYSNQDVAKFVNVLMTRGKKSVAERIIY
                     GALEQIAKKTGKDPIEVFSQAISNVRPMVEVKSRRVGGANYQVPVEVRPVRRTALAMR
                     WLRDAARGRGEKSMGARLASELAEAAEGRGGAIKKREEVHRMAEANKAFSHYRF"
     gene            complement(2935..3312)
                     /gene="rpsL"
                     /locus_tag="VLL03_04860"
     CDS             complement(2935..3312)
                     /gene="rpsL"
                     /locus_tag="VLL03_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HSS46728.1"
                     /translation="MPTINQLVRKPRTAQRPKSKVPALGNSPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGHEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHMVRGSL
                     DTAGVKDRKQGRSKYGAKLAKAT"
     gene            complement(3400..>3785)
                     /locus_tag="VLL03_04865"
     CDS             complement(3400..>3785)
                     /locus_tag="VLL03_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46729.1"
                     /translation="DTYFILGEQVERAEVLEENDNAVAAGKKPAIFDYMLLGITKASL
                     STDSFISAASFQETTRVLTEAAIMGKKDELRGLKENVIVGRLIPAGTGLAYHNTRRKQ
                     AVGAAIEAPAGAESATTTQGEEHAA"
BASE COUNT          649 a         1179 c         1043 g          914 t
ORIGIN      
        1 gtagtcagcg tgccccggac agtccacatg cgcgtagtgg cgcttggagg tctcatattc
       61 cacatgcgcg gtgttgatgg taatcccccg cgccttctct tccggagcgg aatcaatctg
      121 gtcgtagctt ttcacctcgc ccccgtactt cttcgccagc accatggtca tcgccgccgt
      181 caacgtcgtc ttgccatgat ccacatgccc aatcgtcccc acgttcacgt gcggcttggt
      241 ccgctcaaat ttgccttttg acatgatgat gtcctcttat ttcttaacgt ctttgccggt
      301 gaatttattg atgatagcct ccgcgacctg cttcggcgct tccacataat gcttgaattc
      361 catggtgtag gtggcgcgtc cctgagtcag cgagcgcaag gtggtggaat aaccaaacat
      421 ttccgccagc ggcacttcgg ccttgatgac catgatgccg gcgacgtctt cctgaccctg
      481 caccatgccg cgccgcgagc tcaaatcgcc tatcacgttg ccgacgaaat cgccgggggt
      541 ctccacttcc accgccatca tcggctcgag cagcgtcgga ctggctttgc gcatgccgtc
      601 cttgaaagca aaggaggccg ccatcttgaa cgcgttttcg ttggaatcca cttcgtggta
      661 ggaaccgtca aacaaggtga ctttcacatc cactaccggg aagccggcga gcacgccgtt
      721 gggcagcgtc tcgaccaagc ccttttgcac cgcggggata aattcgcgcg gcaccgcgcc
      781 acccttgatc gcgtccacaa attcatagcc cttgcccggg ttcggctcca ccttcagcca
      841 cacatggcca tactggccgc ggccgccgga ttgcttgatg aacttgcctt cgacgtcatc
      901 caccattttc ttgatggttt cgcggtatgc gacctggggc ttgcccacgc tcgcttccac
      961 accgaactcc cgcttcatgc ggtcaacgat gatttcgagg tgcagctcgc ccatgccgga
     1021 aataatcgtt tgcccggatt cctcatcggt gcgcacgcgg aacgagggat cttcctgcgc
     1081 caggcgattg agcgcgatgc ccattttctc ctggtcggcc ttggtcttcg gttccaccgc
     1141 ctgggaaatc accggctcgg ggaataccat gcgctccagc gtaatgattt tccccgggtc
     1201 gcacagcgtg tcgccggtgc tcgcttccct aagccccacc gcggcggcga tgtcgccggc
     1261 gcgcacttcc ttgatttcat cgcgctggtt ggcgtgcatc tgcaatagcc gtccgatgcg
     1321 ttccttctta cccttgatcg ggttgtaaat ggtgtcgccg gaggtcacca cgccggaata
     1381 gacgcggaag aaaatcaact gcccgacata gggatcggtt gcaatcttga acgcaagccc
     1441 cgagaacggc tcgtcatcgg aagcatggcg ctcgccgggc tggccgtttt ccagctcgcc
     1501 tttgatcgcg ggaatgtcca ctggcgctgg catatagtct atcaccgcgt ccagcatcgc
     1561 ctgtacgccc ttgttcttga aggcggaacc gcacagcatg ggcacgattt cgttgttgat
     1621 ggtgcgttgc cgcaggcctt tcctgatttc ctcctcggtg agcgccactc cttcgagata
     1681 cttgtgcatc agctcttcgg aagcctctgc ggcggtttcc accattttct ctcgccagtg
     1741 cttgcactct cccagcatat tggccgggac atcgcgcatc tcaaatttca tgccctgggt
     1801 gctgtcgtcc cagtaaatcg ccttcatttt caccagatcg accacgcctt cgaatttttc
     1861 ctcggcgcca atcggcagtt gaatcagaac cggattggct ttgaggcggg atttcacctg
     1921 gtcgtaaaca cgcatgtagt tcgcgccctg gcgatccatt ttgttgacga acgccaggcg
     1981 tggcacgtgg tatttgttgg cctggcgcca aacggtttcg gtctgcggct gcaccccgcc
     2041 caccgcgcac aacacggtgc aggcaccatc gaggacgcga agtgagcgct ccacttcaat
     2101 ggtgaaatcc acgtgccccg gggtgtcgat aatgttgatg cgatgctcgg gatggttgtg
     2161 atccatgccc ttccagaagc aggtggtggc ggcggaggta atagtaatgc cgcgttcgcg
     2221 ctcctgctcc atccagtcca tgatggtagt accgtcatgc acttcgccca gcttgtggga
     2281 gacgccggtg taaaacaaga tgcgctcggt ggtcgtggtc ttaccggcat caatatgcgc
     2341 ggtgatgccg atattgcgat agcgctcgat aggggttttg cgtgccacgg ctaaattcat
     2401 cctggagggt tactgcgatt agaagcggta atgcgagaag gctttgttgg cttccgccat
     2461 acggtgcact tcctcgcgtt ttttgatggc accgccacgg ccttccgccg cttcagcgag
     2521 ttcgctcgcc agccgcgcac ccatggattt ttcgccgcgg ccgcgcgccg cgtcccgcag
     2581 ccagcgcatc gccagcgctg tgcgccgcac cgggcgcact tccaccggca cctggtagtt
     2641 ggcgccaccg acgcgacggc ttttcacttc caccatcggc cgcacattag aaatcgcttg
     2701 gctgaacact tcaatcgggt ccttgccggt cttcttcgca atctgttcca gcgcgccgta
     2761 aataatccgc tccgcgaccg attttttgcc ccgcgtcatg agcacattga caaacttggc
     2821 cacatcctgg ttgctgtact tgggatcggg cagtacgtgg cgcttgggaa tttctctgcg
     2881 tcgcggcatg gttttaactg tctgggtagg gtctatatat ataaggtctg aacgtcaggt
     2941 ggccttggcc agtttggcgc cgtatttgga gcggccctgc ttgcggtcct tcaccccggc
     3001 ggtatccagg cttccgcgca ccatgtggta gcgcacgccc ggcaggtctt tcacgcggcc
     3061 gccgcgaatc agcaccaccg aatgctcctg caggttatgc ccttcgccgc cgatgtagct
     3121 gatcacttca tggccgttgg tgagccggac tttcgccact ttacgcagcg ccgaattggg
     3181 ctttttcggc gtggtcgtat acacgcgcgt gcaaacgccg cgccgctgcg ggctgttgcc
     3241 cagcgccggc accttgcttt tggggcgctg cgcggtcctc ggcttacgta ccaattggtt
     3301 gattgtcggc atttaacgtc cgtctctaaa aaatacggac ggcgtttgtc tagcgccgcc
     3361 cgaaaagact gtggatttta ttgacttaac aacaacctgt caagccgcgt gctcctctcc
     3421 ttgcgtggtt gtcgcgcttt ctgcgcccgc tggtgcctca atcgccgccc ccaccgcttg
     3481 cttgcggcgg gtgttgtgat aagcgagccc ggtaccggcg ggaatcaggc ggccgacaat
     3541 cacgttttcc ttcaaaccgc gcaattcatc ctttttgccc atgatcgccg cttccgtcag
     3601 cacccgtgtg gtctcctgga aggaggccgc ggagatgaag gaatcggtcg aaagcgaagc
     3661 cttggtgatg cccagcagca tgtaatcgaa tatcgcgggt ttcttgcctg ccgccacagc
     3721 attgtcgttt tcttccagca cttctgctcg ctccacttgt tcgccgagaa tgaagtaagt
     3781 gtcgc
//
LOCUS       DATIOW010000146         2755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_66011, whole genome shotgun sequence.
ACCESSION   DATIOW010000146 DATIOW010000000
VERSION     DATIOW010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66011"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VLL03_04870"
     CDS             complement(<1..514)
                     /locus_tag="VLL03_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019446942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HSS46730.1"
                     /translation="MADAPRDVELPDIPEAVAVPKSRWSIQLVWLIPLVAALIGGWLA
                     VKAVLERGPMITISFKTAEGLEAGKTKIKYKEVEIGVVKSVTLSEDRTRVIATAEIVK
                     EAENLLVEDIRFWVVRARISGGSVSGLGTLLAGSYIGVDIGKSVKPQRNFVGLEVQPL
                     FATDVPGRQFV"
     gene            complement(507..1139)
                     /locus_tag="VLL03_04875"
     CDS             complement(507..1139)
                     /locus_tag="VLL03_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HSS46731.1"
                     /translation="MTATHTTAASLGLLVCYTCGLHSRPAPGIHESKCPRCGAYLHLR
                     KSASIARTWALLIAAAILYIPANVLPILETSSLFDSQTDTILSGIVFLWTTGSWPLAV
                     VVFIASIMVPLLKLIGVTLLVVSVQRRSKWLPEQRARLYRLMEFIGRWSMLDIFVVAI
                     LVTLVQLQALGSIRAGPGAIAFGAVVVLTMFATITFDPRLIWDPLENKHG"
     gene            complement(1136..1750)
                     /locus_tag="VLL03_04880"
     CDS             complement(1136..1750)
                     /locus_tag="VLL03_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HSS46732.1"
                     /translation="MHAAPLIACRECDMLQRETQLPRNGVACCVRCGAMLYRSHPPDS
                     LDRTLAFMLGAIVLFVTANSFPIVGIEVQGQHTEATLYGAVRVLYDQDMRLVAGLVFF
                     TTMLVPTLEAAAICYLLLPLKFGRVPYQFALVFRLLQAVRPWGMIEVFMLGILVALVK
                     LAHIASVVPGTAMWAFGGLMLLIAAVTAAFDPRQLWAALDNMAK"
     gene            2118..2672
                     /locus_tag="VLL03_04885"
     CDS             2118..2672
                     /locus_tag="VLL03_04885"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HSS46733.1"
                     /translation="MSKPLLTIWTIGHGTRSGDELVQMLQEAGVQCLVDVRSYPGSRR
                     NPQFSREPLASLLAKHGIQYQWEGEGLGGFRKPRPDSKHVALRSEGFRGYADYMESEK
                     FKQAIELLIAQAKQTKSAYMCAERLPWQCHRYLISDYLVMRGLRVTHLVALDNAQEHA
                     LNSIARVDQGMLVYDGKMQLGLGL"
BASE COUNT          614 a          807 c          793 g          541 t
ORIGIN      
        1 ccacgaactg ccggcccggc acgtcggtag cgaacaacgg ctgcacttcg aggccaacaa
       61 agttgcgttg tggcttcacg gatttgccga tgtcgacccc gatataggag ccggcaagca
      121 gagtcccgag tccggatacg ctgccaccgg agatgcgcgc acgcacgacc cagaaacgga
      181 tatcctcaac gagcaggttc tcggcctcct tgacgatctc agccgtggct ataacccgcg
      241 tgcggtcttc ggaaagcgtg acgcttttca ccacgccgat ttcaacctcc ttgtacttga
      301 ttttggtctt tccggcttcc agcccctcgg cggttttgaa gctgatggtg atcatcggcc
      361 cgcgctccaa caccgctttc accgcaagcc agccgccgat cagcgccgca accagtggaa
      421 tcagccagac gagctgtatc gaccagcgtg atttcggcac ggcgaccgct tccggaatat
      481 cgggaagttc gacgtcgcgc ggggcgtcag ccatgcttgt tctccaaagg atcccagatc
      541 agccgcgggt caaaggtgat cgtggcgaac atcgtcaata ccactacggc gccgaatgca
      601 atcgcgcccg gtccggccct gatgctcccc agtgcctgca gctgcaccag ggtgacgagg
      661 atggcgacga caaagatgtc aagcatagac cagcgcccga tgaattccat caaccggtac
      721 agacgcgcgc gctgctcggg aagccacttg gagcggcgct gcaccgaaac cacgagcaga
      781 gtcaccccga tcagcttcag caagggcacc ataatgctgg caatgaagac cactacggcg
      841 agcggccatg agcccgtggt ccagaggaaa acaattccac tcaggatggt gtctgtctgc
      901 gaatcgaaca gcgagctcgt ttccaggatc ggcagcacgt tcgctggaat gtaaagaatg
      961 gcggcggcaa tcaacagcgc ccaggtacgg gctatgctcg ccgatttgcg caagtgcaga
     1021 tacgcaccgc agcgcgggca cttcgactca tgaatgcccg gcgccggcct cgaatgcagt
     1081 ccacaggtat aacagaccaa aaggccaaga cttgccgcgg tggtgtgcgt tgcagtcatt
     1141 ttgccatatt atccaacgct gcccacagtt gacgcgggtc gaacgcggcg gtcacggcgg
     1201 caatcaacaa catcaggcct ccgaatgccc acatcgccgt gccgggaaca acgctcgcga
     1261 tgtgcgcgag cttgaccagg gcaacgagga taccgagcat gaacacttct atcatgcccc
     1321 acggccgcac cgcttgcagc aggcggaaga cgagagcgaa ctgatacggg acccgcccga
     1381 acttgagcgg cagcagcagg tagcagattg cggcggcttc cagcgtgggc accagcatcg
     1441 tggtgaaaaa caccaggccg gcaaccagcc gcatgtcctg gtcgtagagc acgcgcaccg
     1501 ctccgtacaa tgttgcctcg gtgtgctggc cttgaacctc aattccgacg atgggaaacg
     1561 aattggcggt gacgaacagt acaatcgcgc cgagcatgaa cgcgagcgtg cggtcgaggc
     1621 tatcgggggg gtggctgcgg taaagcatcg cgccgcagcg cacgcagcac gctaccccgt
     1681 tgcgcggcag ctgcgtttcg cgttgcaaca tatcgcattc gcggcaggca atgagaggcg
     1741 cagcgtgcat tcgtctacct cggcgttgat ttactcttta atccggaact gccgcctgca
     1801 ggcgcaggtt ctgccgcggt catgcgttcg gcgtcgagca cctctgatgt tttttgtatt
     1861 ttcaattatt ttaacgcttt ttattgctat ttttatgtta acactttcta atcaagcccg
     1921 ctaataccca agtgccctca aaattatgcg caaatgcaaa aagctgatcg aagtcgcgca
     1981 caatttcaat cctgtcggca tccctccaca tgggcagcgc ctcctgcgcg aaccttggcc
     2041 gcccggtttt tgcaaaccct ggagcttacg cgtagtttga cttagcatat aaggaagata
     2101 cggccttcgt cagtaaaatg agcaaaccgt tactcactat ctggactatc ggccacggca
     2161 cccgcagcgg cgatgaattg gtgcagatgt tgcaggaagc gggggtgcaa tgcctggtgg
     2221 acgtcaggag ctatcccggc tcgcgccgca acccgcaatt ttcccgcgag ccgctggcat
     2281 ctttgctcgc taaacacggc atccaatatc aatgggaagg tgaggggttg ggcggttttc
     2341 gcaaaccgcg gccggattcc aagcatgttg cgctcagaag cgaaggcttt cgcggctacg
     2401 ccgattacat ggaatcagag aaattcaagc aagcaatcga gcttttaatc gcgcaagcga
     2461 agcaaacaaa gagcgcttac atgtgcgcgg agcggctgcc ctggcaatgc caccgttacc
     2521 tgatttcgga ttacctggtg atgcgcggtt tacgggttac tcatctcgtt gctcttgaca
     2581 atgcccagga gcatgcgctg aattccattg cgcgcgttga ccaaggcatg ttggtatatg
     2641 atggcaagat gcagctcggg ctgggcttgt gaccgacgct tggaaactgc ggtagaattc
     2701 cggcaggcgg gtgctgaagc gtatcggaag ctctgaatga gaaagaggtc ccgcc
//
LOCUS       DATIOW010000147         2249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_981244, whole genome shotgun sequence.
ACCESSION   DATIOW010000147 DATIOW010000000
VERSION     DATIOW010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981244"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1548)
                     /gene="dnaE"
                     /locus_tag="VLL03_04890"
                     /pseudo
     CDS             complement(1..1548)
                     /gene="dnaE"
                     /locus_tag="VLL03_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478208.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
     gene            1624..1863
                     /locus_tag="VLL03_04895"
     CDS             1624..1863
                     /locus_tag="VLL03_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46734.1"
                     /translation="MKKSYALLLLLAACAQEPPKPSQTPNINLSGFPKSFQDGYRDGC
                     TSAQSLLNKKDQARYKSDAQYMTGWQDGYSACKNK"
     gene            complement(1865..>2249)
                     /locus_tag="VLL03_04900"
     CDS             complement(1865..>2249)
                     /locus_tag="VLL03_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HSS46735.1"
                     /translation="LESVEEQISVFDSLPTDVQVGLLKQTVTHHDLIPGMVEKSIQAY
                     LKRDLGAMWDINNSIMETDTDKRFNETFLQRVLYDRNVRMVERLQPRLEEGDVFVAVG
                     ALHLYGSKGVLSLLKARGYQITRMY"
BASE COUNT          464 a          654 c          638 g          493 t
ORIGIN      
        1 ccgcgtgcat gccaacattg cgcgttaagc cttccactcg ttccgccagc gccagcagct
       61 cgtgcacctc ctcttctttt tgctcgcgct ccttgagctg cggctccatc tcgcgcgcca
      121 tttgcagcgt tatggtcttg cccggctgga tcggaatgag tttcgccagc tggtcgcaga
      181 agttgtaggg caaatcgagc acgcgaccaa cgtcacgcac cgccgctttc gccgccatgg
      241 tgccgaacgt ggcgatttgc gacacgctgt cctcgccgta tttgtgcttg acgtaatcaa
      301 tcactttctc gcgcccgtcc tggcagaagt ccacgtcgaa atcgggcatg gaaacccgct
      361 cggggttaag aaagcgttcg aacagcaggt catagcgcaa ggggtcgaga tcggtgatgc
      421 ctagtgcgta ggcaaccagc gaacccgcgc cggagccgcg cccgggaccc acaggtacgc
      481 cgttttgctt cgcccagttg ataaaatccg cgacaatcag gaaataaccg gaataaccca
      541 tttgcacgac ggtgcgggtc tcgaactcca gtcgttcccg atatttggat aactgcttct
      601 cgcgctcttt ggcgtccgga taaagcgttt ccaggcgctg tttgagccct tgttgcgcgc
      661 gctggcttaa gtattcgtcg aggctcacat tgtttggcgt ggggaaaagc ggcagacggc
      721 ttttgcccag ttcgattttg agattgcagc gcttggcgat ttccacgctg ttggcaagcg
      781 cttcgggaat atcggcgaag agctccgcca tctccgcctg agtcttgaaa tattgctgct
      841 cggtaaactg ccgcgggcgt cgctggtcgc cgagcacata accttcggcg atgcacaccc
      901 gcgcttcgtg cgccttgaag tcatcggctt cgagaaactg caccgggtgc gtcgccacca
      961 ccggcagctt gagattggag gcaagttcca gcgcacgctg cacatagtgc tcgccatgcg
     1021 gcgcacccga gcgctgcagc tcgatataaa aacgttgcgg gaaaagcgct tcccactctt
     1081 tcgcaagttt ttccgcctgt ttgcggttat cctgcaggag cgcgatgccg atgtcgcctt
     1141 gcagcgcgcc ggaaagcgca ataggccctc ggtgggcgct tcggcgaacc agattttgcg
     1201 gatttcaggg cgtccgcggt gctggttgct gcggtaagcg cgtgaaatca agcggcagag
     1261 caagagataa cctagatacg actgggcgag cagcagcagg cggaacggct tgtcgcgatc
     1321 agcttcgttg cttatccaca tatcgcagcc gatgatgggc ttcaagccgg ctttgcgcgc
     1381 cgcttgatag aacttcacca tgccgaacag gttgttgagg tcagtaagcg ccaacgccgg
     1441 catgcggtcg ccggccgcct tgttcactgc ggtgtcgagg cgcacgatgc cgtcgacgat
     1501 ggaatactcg ctgtgcagac gcaggtgaac gaaagcgggc tgaggcatgg tgatataatg
     1561 cggctcgatt gcaaatttta ccaccaacag agtgtcgcga aacggtggat tgaattgtcc
     1621 gcgatgaaaa aatcttacgc gttgctgttg ctgctcgcgg cgtgcgcaca ggagccgcct
     1681 aaaccttcac agacgccaaa catcaatctg tcgggttttc ccaagagttt tcaggacggc
     1741 taccgtgacg gctgcaccag cgcccagtcg ctgctcaaca aaaaagacca ggcgcgttac
     1801 aagtcagacg cccagtacat gacaggctgg caggacggct acagcgcgtg caagaataaa
     1861 taaatcaata cattcgcgta atctggtagc cgcgggcctt aagcaagctc agcacacctt
     1921 tacttccgta caaatgcagc gcgcccacgg cgacaaaaac gtcgccttcc tccaagcgcg
     1981 gctgtaggcg ctccaccatg cgcacattcc ggtcgtaaag cactcgctgc agaaaagttt
     2041 cgttgaagcg cttgtcggtg tctgtttcca tgatgctgtt gttgatatcc cacatcgcgc
     2101 ccaaatcacg cttgagataa gcctggatgg atttttccac catgccggga atcaagtcgt
     2161 ggtgggtcac ggtttgtttt agcaagccca cctgcacgtc cgtcggcagg ctgtcgaaca
     2221 ctgaaatttg ctcttccacg ctttccagc
//
LOCUS       DATIOW010000148         8438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_168854, whole genome shotgun sequence.
ACCESSION   DATIOW010000148 DATIOW010000000
VERSION     DATIOW010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8438
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168854"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(543..932)
                     /locus_tag="VLL03_04905"
     CDS             complement(543..932)
                     /locus_tag="VLL03_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46736.1"
                     /translation="MKNIKLGLIIVAGILLVAACATPVLNVSNAPVQTGSGQKASLDG
                     MGKAITRAGVALGWQMKTVKPGNIVGTLTLRGHTAVVEVTYDTQTYNITYKDSVNLNY
                     DGKNIHRNYNGWIQNLDRGIRAQLSTL"
     gene            complement(1078..1353)
                     /locus_tag="VLL03_04910"
                     /pseudo
     CDS             complement(1078..1353)
                     /locus_tag="VLL03_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012218269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
     gene            1496..2491
                     /locus_tag="VLL03_04915"
     CDS             1496..2491
                     /locus_tag="VLL03_04915"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HSS46737.1"
                     /translation="MTPFTGWRDPGVGLWSFLLAPWSRHIRRFDIFINFFAYIPLGFL
                     LSLVLQSRLSIKAALTVSTLCGFTLSLGMEAVQIYLPARSSSTLDLLTNGLGGFAGAL
                     IAAREDVRGLIAVRLAGFRDRWFIHGSLGDLGLVLLGLWFVSQLNPSLPLLGIVFFSE
                     SVNPVAHRFSLIGSLSVMCNLVSIGLLLMALMRTARAAIIATLVLLLAASVIKLLAAA
                     VLLKPEALLQWLTAESVIGIGYGLFIVAASLLLTIRQIIPVCAFALAAGIFVAHLHSG
                     EPRSSLALHLFSWHYGHLLNFTGLARITAELWPFAALGYLCLLVQRLRAGIRHRV"
     gene            2525..2833
                     /locus_tag="VLL03_04920"
     CDS             2525..2833
                     /locus_tag="VLL03_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HSS46738.1"
                     /translation="MSHYQRHVFFCCNQRESGETCCNNHHANDLRTYAKEKIKALKLA
                     GPGKIRINKAGCMDRCNEGPVLVVYPEGVWYTYVDQHDIDEIVEQHLCRGRIVERLKI
                     "
     gene            2849..3460
                     /locus_tag="VLL03_04925"
     CDS             2849..3460
                     /locus_tag="VLL03_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSS46739.1"
                     /translation="MHPSTLRKLFIDGPAGKLETVIDDPAEARRGIALIAHPHPLFGG
                     TLDNKVVQTLAKTFVELGYVALRPNFRGAGKSAGAFDEGRGEIEDMLAVAGFAQREFG
                     NLPFLLAGFSFGAFVQSRVMTRLPAEKLVLIAPAVERFDVDAVPANTLVVHGEEDDVV
                     PLASVLDWARPQQLPIVLLPGTGHFFHGRLTQLKQIVLDACKS"
     gene            3448..4359
                     /locus_tag="VLL03_04930"
     CDS             3448..4359
                     /locus_tag="VLL03_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSS46740.1"
                     /translation="MQVLSVRGLRKSYAALEVVAGIELSIGRGECFGLLGPNGAGKTT
                     TLRLCLGLNEPNAGEISMLGFPVPPQARQARLRVGVMPQHDNLDPDFTVRENLLVYGR
                     YFGLPDPIINSRIPALLDFAGLAARANAKISALSGGMKRRLTLARALVNDPDLIFLDE
                     PSTGLDPQARHLIWDGLKKLLNRGKTILLTTHFMDEAERLCNRIGIMDHGRIIASGAP
                     RDLIAKHIEAQVVEVFGEDVEKWLEQHGQKLAQRCERTGETVFCYTGEAQPLIDSLRN
                     VSRLRYLHRPANLEDVFLKLTGRELRD"
     gene            4359..5147
                     /locus_tag="VLL03_04935"
     CDS             4359..5147
                     /locus_tag="VLL03_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSS46741.1"
                     /translation="MPNRKYFRPPQISLRFIPVWRRNFLVWKKLAIPSILGNLADPLL
                     YMLGLGYGLGVLLPQINGMSYITFLAAGTLCYSTMNTASFEALYSAFARMHVQKTWDA
                     ILHAPLTLDDVLFAEMVWASSKSFLSGISILLIIWVLGLSHSWLMLLLIPLIVLIGLS
                     FAGFGLIMTSVCPSYEFFLYYFTLFITPMVLLCGVFFPVSQLPPFLREISSYLPLTHA
                     IELARPLVSGRVPERALMHILVLAGSGAVGFYVALVLTRRRLLK"
     gene            5273..5683
                     /gene="hemJ"
                     /locus_tag="VLL03_04940"
     CDS             5273..5683
                     /gene="hemJ"
                     /locus_tag="VLL03_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HSS46742.1"
                     /translation="MLWIKAFHIISVIAWFAGLFYLPRLFVYHSAAEDSISLERFITM
                     ERKLYFYIMTPAAVLALGFGFWLLGYGFSGMWLNLKLVLVIALILFHMFCGKLLIDIK
                     NDPKSHGRKFYRWVNEIPTVILISIVILVVVKPF"
     gene            5719..6870
                     /locus_tag="VLL03_04945"
     CDS             5719..6870
                     /locus_tag="VLL03_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HSS46743.1"
                     /translation="MEKFFASCPRGLEKPLAAELQSLGAARLEATDGGVAFAGEFLLA
                     YMVNLHSRIASRVLWLLARQRYQTEEDVYRAAHALPWADWFEPELTLRVNVSAIHCPL
                     PSLDFVTLKIKDAVCDRFREETGRRPSVDTQQPDLRIHAFLDAGEIFLYLDTSGEPLF
                     KRGWRKVQGEAPLRENLAAGILHLASWSPGIPFLDPMCGSGTFLMEAALMSFNMAPGM
                     GRHFAFEKLKNFNARKWKMLLHESKQGQKAKTLLPVYGSDLYGEALKHARANLAAAGL
                     EGTVTLKQVNVLEITAPTSAGIMVTNPPYGKRVGEIDELEKFYPQLGDVLKKKFSGWN
                     CYIFSADVRLPKLIRLNASKRTPLHNGPLECRLFEYKMVAGSMRKKREQ"
     gene            complement(6949..8085)
                     /locus_tag="VLL03_04950"
     CDS             complement(6949..8085)
                     /locus_tag="VLL03_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HSS46744.1"
                     /translation="MEREQAVKFMNDLLRLMLSKKASDLFITAGFPPAIKVDGKVTPV
                     SSQALTPQHTQEFARALMNDRQTQEFEATKECNFAINPPGIGRYRVNAFVEQARVGLV
                     LRTITTTIPKFEELNLPAVLKDIAMTKRGLVIFVGGTGCGKSTSLAAMVDYRNENSQG
                     HIITIEDPIEFVHEHKNCIVTQREVGVDTDNWFSALKNMLRQAPDVIQIGEIRERETM
                     DYAIAFAETGHLCMATLHANSTNQALDRIINFFPEERRQQLLMDLSLNIRAFVSQRLI
                     PKKEGKGRVAAVEIMLNSPLISDLIFKGDVHEIKEIMSRSRELGMQTFDQSLFDLYEA
                     GQISYEDALRNADSVNDLRLRIKLHGSEAKDKDIMSGIDHLNIV"
     gene            complement(8110..>8438)
                     /locus_tag="VLL03_04955"
     CDS             complement(8110..>8438)
                     /locus_tag="VLL03_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HSS46745.1"
                     /translation="VIDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGSGRVAAFEV
                     MIGTPAIRNLIRENKVAQMYSSIQTGQNVGMQTMDQNLMELLKRGVVTSEEARSKAAN
                     KDTFFG"
BASE COUNT         1680 a         2411 c         2306 g         2041 t
ORIGIN      
        1 ggccgaggcc ctcaccaaac aagggggcat ccggatcaat ttgctccggg gtgatttcca
       61 ggttcagcgc atccacgatg agctgcgtca cttctctttc caggtcctgt attttttcca
      121 ttctcctcgg tccccgcacg cgctcattgc tgcggcggat tgtccttaaa atgcgactta
      181 atcattatca ttctaactgc cgctggttaa gccctacaag gcgcgcacta aatatataca
      241 taacagggta attttttgct tctactgtcg ttttggaagc cgcgaacagc caagcgcaaa
      301 gccggctgaa aacacaagta ggataggggc aagacatact gccggcttgg agcccgataa
      361 tcaggcaagg taagcaccac ggccccgccc ctgcttcccc acaacggcag ctgctctgac
      421 tgcgctagca cgggcaagca aagtgtataa atcgccgatg cgcacctaac tataatactt
      481 tttcaataaa caacccgcac ccctgggcac cggccaatag caggctggct gtgatgcggg
      541 aattaaagtg tgcttaactg cgcgcggata cctctgtcaa gattctgtat ccagccattg
      601 tagtttcgat gaatattttt tccgtcgtaa ttcaaattga cgctgtcttt gtaggtaatg
      661 ttgtaggtct gcgtatcata agtaacctcc accacagcgg tgtggccacg cagggtaagc
      721 gtgcccacga tattgcctgg cttcaccgtt ttcatttgcc agcctaatgc cacacccgcg
      781 cgggtgatag ccttacccat tccatccaag gacgcttttt ggccgctgcc ggtttgtacc
      841 ggcgcattgg agacatttaa taccggtgtt gcgcatgccg ccactaacag gatacccgcg
      901 acaataatca gccctaattt gatgtttttc atgattttcc cttttagctt gtcagcctaa
      961 acgcagggca cgtaaaaaat tctatgcgca gcccttctct aaaattgcgt agcgcatccg
     1021 ccgcgcttaa tggattatat ggagaaccgg tgcagtgtca acgatggcgg cgcaaagccg
     1081 cgggtgccgc ggaatcttgc cggcgaaatg gtggtcgaaa taatagacct gtgctccgcg
     1141 cttgagcact cgcagcaatg ccggttggtt ttccttgagt gagatgtcca ataccgtgag
     1201 tacatctccg gcttgagctt gcactttttc caggaggcgg atatcgcgtt ttactccggt
     1261 aaccagaata ctttcacgcg gaaaactcag ccgcagttgg cgcaacgcgc aaaggccgtc
     1321 ggcgtcgcca ttaaatacgt catagaatgc cattacaact gctctgaatg tgcttgtgga
     1381 aaaaacaata atggattcga cactggcaat gaattaatcg gtccctcgtc cgcgcggcgc
     1441 caggttcgcc gcctatctcg cgctagctta tctactcatc atcatctatg ccagtttgac
     1501 gccgtttacc ggctggcggg atcccggcgt gggtctctgg tcgtttctgt tagcgccctg
     1561 gtcgcgtcat atcaggcgct tcgacatttt cattaatttc ttcgcctata ttccgttggg
     1621 ttttctgtta tcgctggtgc tgcaatcgcg tttaagcatc aaggctgcac tgacggtttc
     1681 caccctttgc ggattcacac taagcctggg gatggaagcg gtgcaaatct atttgccagc
     1741 ccgcagcagc tccaccctgg atcttctaac caacgggttg ggcggcttcg ccggcgcact
     1801 catcgccgca cgcgaggatg tgcgcgggct gattgccgtc cgtctcgccg gattccgcga
     1861 ccgctggttt attcacggaa gcctgggcga tctggggctg gtgctgctgg gattatggtt
     1921 tgtcagccaa ttgaatccat cactgccgtt gctgggcatc gtattttttt ccgagagcgt
     1981 gaatcccgtt gctcaccgct tcagcctcat cggcagtctc tcggtcatgt gcaatcttgt
     2041 aagcatcggt ctgctcctga tggcgctgat gcgcaccgcc agggcggcca tcatcgccac
     2101 gctggtatta ttattggcgg cctccgttat caagcttctg gccgcggcag tgctattgaa
     2161 gccggaagcg ctgctgcagt ggcttaccgc cgaatcggtc atcggcatcg gttatggctt
     2221 gtttatcgtc gccgcatcgc tgctgcttac aattcgtcaa atcattccag tgtgtgcttt
     2281 tgcgctggcg gcgggcatct tcgtcgccca cctgcattcg ggtgaacccc gttcgagcct
     2341 ggcgctgcat ttgttcagtt ggcattacgg gcatctgctc aatttcaccg gcctggcgcg
     2401 gattaccgct gaactgtggc cgtttgccgc gctcggttat ctctgcctgc tggtgcaacg
     2461 actccgcgcg ggaatccggc atcgagtata atggatcggg tcgcgtttgc actcggccgg
     2521 cgccatgagc cactatcaaa gacatgtgtt tttctgctgc aaccagcgcg agtccggcga
     2581 aacctgctgc aacaatcatc acgccaatga tttgcgcacc tacgccaagg agaaaatcaa
     2641 ggcgctgaag ctcgcgggcc ccggtaaaat ccgcatcaac aaggcgggct gcatggaccg
     2701 ctgcaatgaa ggtccggtgc tggtggttta cccggaaggc gtgtggtaca cctatgtcga
     2761 ccaacacgac attgatgaaa ttgtcgaaca acatctttgc cgtgggcgca ttgtcgagcg
     2821 cctgaaaata tagagcgctt ttgccgcatt gcatccctcc accctgcgca aactgttcat
     2881 cgacgggccg gcgggcaagc tggaaaccgt catcgacgac cctgctgaag cgcgccgtgg
     2941 cattgcgctg attgcccacc ctcatcctct gtttggcggt accctggaca acaaagtggt
     3001 gcagacactc gccaaaactt ttgttgagtt gggatatgtc gcgctgcgcc ccaatttccg
     3061 cggcgcggga aaaagcgccg gcgcttttga tgagggacgc ggcgaaatcg aagacatgct
     3121 cgccgtcgcc ggattcgccc agcgcgagtt tggcaatttg cctttcctgc ttgccggttt
     3181 ttccttcggc gccttcgtgc aatcgcgcgt gatgacgcgg ctgcctgcgg aaaagctggt
     3241 gttgattgcc cccgcggtag agcgttttga cgtcgacgca gttccggcaa atacgctggt
     3301 cgttcacggc gaagaagacg atgtggtgcc cttggcctcc gtgcttgact gggcgcgccc
     3361 gcagcagcta cccattgttt tgctgcccgg caccgggcat ttctttcacg ggcggctcac
     3421 ccagctcaag caaatcgtac tcgacgcatg caagtcctga gcgttcgagg tttacgcaaa
     3481 tcctatgccg cgctggaagt cgtggccgga attgagcttt cgatcgggcg cggcgaatgt
     3541 tttggtctgc tcggccccaa cggcgccggc aaaaccacta ccttgcgcct gtgcctggga
     3601 ctcaacgaac cgaatgcggg cgagatttcc atgctaggtt tcccggtgcc gccgcaagcc
     3661 aggcaagcgc ggctgcgggt gggcgtcatg ccgcaacacg acaaccttga cccggatttc
     3721 accgtgcgcg aaaacctgct ggtgtacggc cgttatttcg ggctccctga cccgataatc
     3781 aactcgcgca ttcccgctct tctcgatttt gccggactcg ccgcgcgcgc caacgccaaa
     3841 atctccgctc tttccggcgg catgaagcgg cggctcaccc tggcgcgggc gctggtgaac
     3901 gatcccgact tgatttttct cgacgagccg agcaccggcc tcgatccgca ggcgcggcat
     3961 ttgatttggg acggtttgaa gaaactcctg aaccggggca aaacgatttt gctcactacc
     4021 catttcatgg acgaggcgga gcggctgtgc aaccgcatcg gtatcatgga tcatgggcgc
     4081 atcatcgcca gcggggcgcc gcgcgatttg attgcaaagc acatcgaagc gcaggtggtg
     4141 gaggttttcg gcgaagacgt ggagaaatgg ctggaacaac acgggcaaaa attggcgcag
     4201 cgctgcgagc gcaccggaga aaccgttttt tgctacaccg gggaagcgca acccctcatt
     4261 gacagcctgc ggaacgtaag ccgtctgcgc tatctccacc gccctgccaa cctggaggat
     4321 gtgtttctga agctgacggg gagagagctg cgcgactgat gccgaaccgc aaatactttc
     4381 ggccgccgca gatcagcctg cgcttcattc cagtatggcg gcgtaatttt ctggtatgga
     4441 aaaagcttgc cataccctcg atccttggca accttgccga ccctttgctt tacatgttgg
     4501 gattgggtta cggcctcggc gttctgttgc cccaaatcaa cggcatgtcc tatatcactt
     4561 ttctcgccgc cggcacgctt tgctacagca ccatgaacac cgccagcttt gaggcgctgt
     4621 attccgcgtt tgcgcggatg cacgtgcaaa aaacctggga tgccattttg catgcgccgc
     4681 tcacgctgga cgacgttctg tttgccgaaa tggtctgggc gtccagcaag agttttttga
     4741 gcggcatctc gattctcctg attatctggg tgcttgggct gtcgcattcc tggctgatgc
     4801 tgctgctcat cccgcttatt gtgctgatcg gcttgagctt cgccggtttc ggtttgataa
     4861 tgacctcggt ttgcccaagc tacgagtttt tcctgtatta cttcactctc ttcatcaccc
     4921 cgatggtgct cctgtgcggg gtattttttc cggtaagcca gttgcccccg ttcttgcggg
     4981 aaatctcctc ctatctgccg ctcacccatg ccatagaact ggcaagaccg ctggtcagcg
     5041 ggcgagtgcc cgaacgggct ttaatgcata tactggttct tgccggatct ggcgcagtcg
     5101 gcttttatgt ggcgctggta ttgacacgcc ggcggctgct gaaataaacc caaaaactcg
     5161 ctaaaccgcc gattaacgca gatgaacgca gatatttcag cagccggaaa aataatctgt
     5221 tttgtcggtg tttatcggcg tgtatcggcg gttaaccgcg gaatttaagt ttatgctctg
     5281 gatcaaagcc tttcatatta tttcggtcat cgcgtggttt gccgggcttt tctacctgcc
     5341 gcggcttttt gtttatcact cggcagcaga agactctatc agcctcgagc gctttatcac
     5401 gatggaacgc aagctctatt tctacatcat gacgccggcg gcggttctgg cgctgggttt
     5461 cggcttctgg ctgctgggtt atgggttctc cggcatgtgg ctcaatctga aactggtgct
     5521 ggtgatcgcg ctgattcttt ttcacatgtt ttgcggcaag ctgctcattg acatcaagaa
     5581 cgaccccaag agccacggcc gaaaattcta ccgctgggtt aacgaaattc ccactgtcat
     5641 tctgatttcg atagtaatcc tggttgtggt caagccgttc tgagtcgtag tattccaatt
     5701 catcactcgt cgctggtcat ggaaaaattc ttcgcgtcct gcccgcgcgg tctggaaaaa
     5761 ccgctggcgg cggaacttca gtcgctcggc gcggcgcggc tcgaagcgac tgacggcggg
     5821 gttgcctttg ccggcgagtt cctgctcgct tacatggtca atttgcacag ccgcatcgcc
     5881 agccgcgtgt tgtggctgct cgctcggcag cgctaccaaa ccgaggaaga cgtttaccgg
     5941 gcggcgcatg cgctgccgtg ggccgactgg tttgagcccg aactgactct gcgggtcaat
     6001 gtttccgcca ttcactgccc tcttcccagt ctggattttg tcacgctcaa aatcaaggat
     6061 gccgtgtgcg accgcttccg cgaggaaacc ggcaggcgtc cgagcgtgga tacgcagcag
     6121 ccggacctac gtatccacgc ttttctcgac gccggggaaa tttttttata tcttgacacc
     6181 agcggcgaac cgctgttcaa gcgcggctgg cgcaaggtcc agggcgaagc gccgctgcgg
     6241 gaaaacctcg ccgcgggaat tctacatctt gcttcctggt cgccggggat tccgttcctc
     6301 gacccgatgt gcggcagcgg cacctttctg atggaagcag cgttaatgtc gttcaacatg
     6361 gcgccgggca tggggcggca ttttgctttc gagaagctca agaatttcaa tgcgcgcaaa
     6421 tggaaaatgc tgctgcatga aagcaaacaa ggacagaaag caaaaactct cctgccggtt
     6481 tatggcagcg acctctatgg cgaggccttg aaacacgcac gcgccaatct ggcagcggca
     6541 ggactggaag gcacggtcac gctcaagcag gtaaatgtgc tggaaattac ggcgccgaca
     6601 agcgcgggga tcatggtcac caatccgccc tacggcaagc gcgtcggcga gattgatgag
     6661 ctggaaaagt tttatccaca gctgggcgat gttctcaaga aaaaattcag cggctggaac
     6721 tgctacattt tcagcgccga cgtgcgtttg ccgaaattga tccggcttaa cgcctcgaag
     6781 cgcacgccac ttcacaacgg tccgctggaa tgccgtctgt ttgaatacaa gatggtggcg
     6841 ggatcaatgc ggaaaaagcg agaacagtga gcagcaaaca gcgaacggtc agaaccagat
     6901 cgtattgcct ccgctccctg ttcaccgttc accgttcacc attcaccatc aaacgatatt
     6961 gagatggtca atgccggaca taatgtcctt gtctttcgcc tccgacccgt gcagcttgat
     7021 tctcaagcgc aagtcgttga ccgagtcggc gttccgcagt gcatcctcat agctgatctg
     7081 ccccgcttcg tacagatcga acagggactg gtcaaaagtc tgcatgccca gctcgcgcga
     7141 tctcgacatg atctctttga tctcatgtac atcgcctttg aaaatcaggt cggaaatcaa
     7201 cggtgagttc agcatgattt cgaccgcagc gacgcgcccc ttgccttctt ttttcggaat
     7261 caggcgttgc gagacgaagg cacggatgtt caacgacagg tccatcagca gttgctgcct
     7321 gcgctcctcg gggaaaaagt tgatgatgcg gtcaagcgcc tggttggtgc tgttggcatg
     7381 caacgtcgcc atgcacaggt gtccggtttc ggcaaaggcg atcgcgtaat ccatggtctc
     7441 gcgctcgcgg atttcgccga tctgaatcac gtccggcgcc tggcgcagca tgtttttcag
     7501 tgcggaaaac cagttgtcgg tatccacccc gacttcgcgt tgggtgacga tgcagttctt
     7561 gtgctcgtgc acgaattcaa tcgggtcctc gatggtgatg atgtggcctt gggaattttc
     7621 gttgcgatag tcgaccatcg ccgccagcga agtcgatttg ccacagccgg tgccgccgac
     7681 aaagataacc aagccgcgct tggtcatggc gatgtccttg agcaccgccg gtagattaag
     7741 ctcctcgaat ttcgggatgg ttgtggtgat ggtgcgcagc accaggccga cccgggcctg
     7801 ctccacaaaa gcgttgacac ggtaacggcc gattcccggc gggttgatcg cgaaattgca
     7861 ttccttggtg gcttcgaatt cctgcgtctg cctgtcattc atcagcgcac gcgcgaattc
     7921 ctgggtatgc tgcggcgtca acgcctggct ggaaaccggc gtcaccttgc catccacctt
     7981 gatcgccggc gggaaacccg cggtgataaa caggtcggaa gcttttttgc tgagcatcaa
     8041 gcgcagcaga tcgttcatga attttactgc ctgctctcgt tccataatct actccttgcg
     8101 atacagcctt tatccaaaaa aagtatcctt gttcgccgcc ttgctacgcg cttcctcgga
     8161 agtcacgaca ccgcgcttca gcagctccat caagttctga tccatggtct gcattcccac
     8221 gttctgtccg gtttgaatcg acgagtacat ctgcgccacc ttgttctcgc ggatgagatt
     8281 tcggatcgcc ggtgtgccga tcatgacttc aaacgctgcc acgcgcccgc tgccgtcctt
     8341 ggtcttgagc agcgtctgcg aaatcactgc gcgcaagctt tccgaaagca tcgagcgcac
     8401 catttccttt tccgccgcgg gaaagacgtc aatgacgc
//
LOCUS       DATIOW010000149         7252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1591627, whole genome shotgun sequence.
ACCESSION   DATIOW010000149 DATIOW010000000
VERSION     DATIOW010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7252
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591627"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1557)
                     /gene="carB"
                     /locus_tag="VLL03_04960"
                     /pseudo
     CDS             complement(82..1557)
                     /gene="carB"
                     /locus_tag="VLL03_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
     gene            complement(1626..2777)
                     /gene="carA"
                     /locus_tag="VLL03_04965"
     CDS             complement(1626..2777)
                     /gene="carA"
                     /locus_tag="VLL03_04965"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HSS46746.1"
                     /translation="MSQRKPAILALADGTIFRGTAIGGEDMAVGEVVFNTAITGYQEI
                     LTDPSYCKQIVTLTYPHIGNTGVNPEDTESHRVFASGLVIRDLPPVSSNWREAQTLPA
                     YLKQQNVVAIADIDTRKLTRILREKGAQNGCIMAGKIDEQEALKAACAFPGLAGMDLA
                     KEVSCKRPFAWNEGEWKLGQGYSRQEQPKFHVAAYDYGIKNNILRKLATRGCRITVLP
                     AQMPAREAMMIKPDGIFLSNGPGDPEPCDYAISTIRELLDIGIPIFGICLGHQLLALA
                     SGARTVKMKFGHHGANHPVLEVDTGRVMITCQNHGFAVDAATLPPNLRTTHVSLFDGS
                     LQGLARTDKPAFCFQGHPEASPGPHDVDPLFDRFVGLIEKHKATADERR"
     gene            complement(2891..3697)
                     /gene="dapB"
                     /locus_tag="VLL03_04970"
     CDS             complement(2891..3697)
                     /gene="dapB"
                     /locus_tag="VLL03_04970"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HSS46747.1"
                     /translation="MTQLKIAIAGSSGRMGRALIEAVLQADDLRLHAAFDITGSPYLK
                     RDAGEFCCSPSQVLVTDDVAGALKGADVFVDFTRPEGTLGHVQLCRKNGVKMVIGTTG
                     FNEPQKKKLNEAARDIAIVFAPNMSVGVNVMLKLVENAAKALGDDYDVEIIEAHHRHK
                     VDAPSGTALRLGEVAAEARGRSLKTSAVYGRQGITGERDSASIGFATVRGGDIVGDHT
                     VLFAGVGERIEITHQASSRATFAMGALRAARFLKSRKNGLFDMQDVLGLR"
     gene            complement(3761..4933)
                     /locus_tag="VLL03_04975"
     CDS             complement(3761..4933)
                     /locus_tag="VLL03_04975"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HSS46748.1"
                     /translation="MRYRLIFLALFLTACSPTVLITPHKIDVQQGNVVTQDMLDKVKP
                     GMTKSQVRFVLGTPLISDPFHANRWDYVYRFNKAGKLTESRKLTAVFEGDKLKEIEGA
                     ALPPSDKIAGTRPVELIVPDKTDQTQPAAVAAAPEQPNPPLPPVEGLKLKLDSNLAES
                     PQSIPAIEAKPATAQQVQQAQTEQPKRGLGERFLRLFGFGKKDEVQQAKAEEKSEAQP
                     EPEAEAEQATNTVVTADRTPPPKRSVGERFMRLIGFKEKVGDDPAPPVTTETQNRPLR
                     IGTMNLKDLVVKQILGFGDDTEKERKALAEDIEPGESEKSGAQGTTWQRFKRLIGVGS
                     EEQEPISAPAKNQPQRKDQQTKTEAPESTKTEGTQPADDRSFFQKTLDAIGLGSKK"
     gene            5007..5432
                     /gene="fur"
                     /locus_tag="VLL03_04980"
     CDS             5007..5432
                     /gene="fur"
                     /locus_tag="VLL03_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HSS46749.1"
                     /translation="MSSPSDLKTIGLKATLPRLKILNLFENSEVRHLTAEDVYKVLTG
                     EGMDIGLATVYRVLTQFEQAGLLIRHHFESGKAVFELNQGGHHDHLVCLQCGRVEEFV
                     DQEIERRQIKIAKERGFTIHEHSLHLYADCTKPNCPHKT"
     gene            complement(5811..>7252)
                     /locus_tag="VLL03_04985"
     CDS             complement(5811..>7252)
                     /locus_tag="VLL03_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSS46750.1"
                     /translation="TEVVALHRDGHEFPMEISFSHMDIGGKPLFAGFMRDITERKLQE
                     EKLNYLAYYDMVTGLPNRALCYDRLNQNLQAARREGTRTAVAFVDIERFREINESLSR
                     QGGDALLKQVGERLTRSFGSAASPGRLAADCFVAMITNVREEAGIVDAIEQKIFQPLN
                     QPFKLGNQELRIAVKVGVALSPHDGDNAETLFRNAEAALGWAKTSGERSLFYAPEMNA
                     RVTEKLALENKLRVALEQQQFVLHYQPKIDLVSRQVSGLEALIRWQDPETGLVPPLKF
                     IPLLEETGLILDVGYWVIRQALVDCQRWAVKGLHPRIAVNVSPIQLRQKDFVDRVKQA
                     TAGYAADTQSLDLEITESLIMENIEDNIRKLKALRDMGVGIAIDDFGTGYSSLGYLAK
                     LPVNALKIDRAFIVNMVQDPDSMTIVSTIISLAHSLNLKVIAEGVDSEEQSKILRLLK
                     CDEMQGFLFSPPVPFAKIETILKENRTLK"
BASE COUNT         1391 a         2150 c         2074 g         1637 t
ORIGIN      
        1 acgaagctca actacgagct gcggtcgccg ggcgcggatc gcatcctgta cgtcggcgac
       61 gcgttccgtg cgggctggtc gctcgcgcag gtgcacgagc tcacgaaatt cctccgcact
      121 caacgcgtcg agcgttttgc ccttgagcgc ctgctcccgc cgcaccagat cttcaatctg
      181 cgccagaaac cacgagtcga tgcgcgtcaa accgtacact tcctccatgc tcatgccgca
      241 gcggaaggcg tcggccacat accagatgcg ctcgggcccg acatcgccca gctcattttc
      301 aatcacgtca cgctcagcgg ttttttcatc gagcccatcg cttccagttt ccaatccgcg
      361 caaggctttt tgcagcgatt cctggaaggt gcggccgatt gccatgactt cgcccaccga
      421 tttcatttgg gtagtcaggc gggtgtcggc ctgtggaaat ttctcaaacg caaaccgcgg
      481 cattttggtc accacataat caatggacgg ttcgaacgaa gccggagtcg cgccgccggt
      541 gatttcgttt ttgagttcat ccagcgtgta gcccaccgcg agctttgccg ctaccttggc
      601 aattggaaag ccggtggctt tcgaggccag cgcgcttgag cgcgacacgc gcgggttcat
      661 ttcgataatc agcatgcgcc cgtcccgggg gttgacggca aactgcacat ttgagccgcc
      721 ggtgtccaca ccgatttcgc gcagcacccg aatcgaagcg tcgcgcatga tctggtattc
      781 cttgtcggta agcgtctgcg ccggcgccac cgtaatcgaa tcgccggtgt gtacacccat
      841 cggatcgagg ttttcaatgg agcagacgat gatgcagttg tcttttttgt cgcgcaccac
      901 ctccatctcg aatttcttcc agccgatcac cgattcttct atcaagagct ccttggtcgg
      961 cgaggcttcg aggccgcgct cgcagatggt gatgagttct tcccggttgt aggcgattcc
     1021 gccgccggtg ccgcccaaag tgaacgaagg gcggaccacc gtggggaacc ccaccatcgc
     1081 ctgcacttgc agcgcttctt ccatgctgtg agcaatcgcc gagcgcggag acgacaaacc
     1141 gatccgggtc atggccagct tgaatttctc gcggtcctcg gccttgtcaa tcgcttcgcg
     1201 cgacgcgcca ataaattcca ctccgtattt ttccaaaaca ccatgcttgg ctagatcgag
     1261 cgcgcagttc aaagcggtct gcccgcccat ggtgggaagc agcgcgttcg ggcgctcgat
     1321 ggcgatgatt ttttccagca tcgaccagtt gatgggctcg atgtaggtgg catccgctac
     1381 ttccgggtcg gtcatgatgg tcgccggatt ggaattaacc agaatcaccc ggtagccttc
     1441 ttctttaagc gccttgcacg cttgcgcgcc ggaataatcg aactcgcacg cctggccgat
     1501 gatgatgggg ccggcgccga tgatcagaat gcttttaatg tcggttcttt tcggcatagt
     1561 tcaaactctt gtaaccgcca atgcacgccg ataaacgcag ataaaaactg aaacgcaatc
     1621 ggcgttcatc ggcgttcatc tgcggttgcc ttgtgctttt cgatcaatcc cacgaagcgg
     1681 tcgaagagcg ggtccacgtc atgcgggccg gggctcgcct cggggtgccc ctgaaagcag
     1741 aacgccggct tgtcggtgcg cgcaagcccc tgcaggctgc cgtcgaacaa cgacacatgc
     1801 gttgtgcgca aattcggcgg aagcgtggcg gcgtccaccg cgaagccgtg gttctgacag
     1861 gtaatcatca cgcgtccggt atcgacttcg agcaccggat gattggcgcc gtggtggccg
     1921 aacttcattt tcaccgtcct ggcgccgctg gcgagcgcca gcaactggtg gccgagacaa
     1981 atcccgaaaa tcggaatacc gatatcgagc aattcgcgta tggtggaaat cgcgtaatcg
     2041 cagggttcgg ggtcgccggg gccgttggaa agaaaaattc cgtcgggctt gatcatcatc
     2101 gcttcgcggg cgggcatttg cgccggcagc acggtgatgc ggcagccgcg cgtggccagc
     2161 tttctcaata tgttgttctt gatgccgtaa tcgtaggcgg cgacgtgaaa cttcggctgc
     2221 tcctgcctgc tgtaaccctg gccgagcttc cactcgcctt cgttccaagc aaacggccgc
     2281 ttgcagctca cttccttcgc caggtccatt ccggcaaggc cgggaaacgc gcacgcggct
     2341 ttaagcgctt cctgctcgtc gatttttccc gccatgatgc agccgttttg cgcacctttt
     2401 tcgcgcagga tgcgcgtgag cttgcgcgtg tcgatgtcgg caatcgccac cacgttctgc
     2461 tgtttaaggt aggcgggcag cgtctgcgct tcgcgccagt tgctggaaac cggcggcaga
     2521 tcgcggatca ccagaccgct ggcaaacacg cggtgtgatt cggtgtcctc cggattcacg
     2581 ccggtattgc cgatgtgcgg gtaagtcagc gtgacaatct gtttgcagta ggaagggtcg
     2641 gtgaggattt cctggtagcc ggtgatggcg gtgttgaaca ccacctcgcc aacggccatg
     2701 tcttcgccgc caatcgctgt gccgcgaaaa atggtgccat cggcgagcgc gaggatggca
     2761 ggcttccgtt gcgacacaaa aaactccgtt tttgcaggtg gatacacaaa gcgggacagg
     2821 ctctgcgccc atcccgcttc tgaagcctag agattatact ctattaacga ccggctatca
     2881 acgcagacgg ctagcgaagg ccaagcacgt cttgcatatc aaagaggccg ttcttcctgc
     2941 ttttgagaaa gcgcgcggca cgcaaagcgc ccatggcaaa ggtcgcacgg ctggaggcct
     3001 ggtgggtgat ctcgatgcgc tcacccacgc cggcaaacag cacggtgtgg tcaccgacga
     3061 tgtcgccgcc gcgcaccgtg gcaaagccaa tgctggcgga gtcgcgctcg ccggttatcc
     3121 cttgtcggcc atagactgcg gaggtcttga gactgcggcc gcgcgcctcc gctgctactt
     3181 cgcccaggcg caaagccgtg cctgaaggcg catccacctt gtggcggtga tgcgcctcga
     3241 tgatttccac gtcgtagtca tcacccaggg ctttcgccgc gttttcgacg agcttcagca
     3301 tgacattaac gccgacactc atgttgggcg caaatacgat ggcaatgtcc ctggcggcct
     3361 cgttcagctt ctttttttgc ggctcattaa aacccgtcgt gccgatgacc attttcacgc
     3421 cgtttttgcg gcaaagctgc acatgcccga gcgtgccttc ggggcgggtg aaatcaacga
     3481 acacgtcggc gcctttgagc gcgcccgcca catcgtcggt gacaagcacc tggctcggac
     3541 tgcagcaaaa ctcgccggca tcccttttta aataggggct gccggtaatg tcgaacgcag
     3601 catgaagccg cagatcatcc gcctgcagca ccgcttcaat cagcgcgcgt cccatccggc
     3661 cggaactgcc ggcaatcgca atcttcaatt gggtcatggc ggttattgtt cagcgttatt
     3721 tgatgtccgg ttccccgccg cccttggtgt cggcaggatt ttattttttg ctgcccaagc
     3781 caatggcgtc tagagttttc tgaaagaaac tacggtcgtc agccggctgc gttccttccg
     3841 ttttggtgga ttcgggcgct tcggtcttcg tctgctggtc cttcctctgc ggttgattct
     3901 tcgccggcgc ggaaatcggt tcctgttcct cgctgcctac gcctatcaag cgcttaaaac
     3961 gctgccaggt agtgccctgt gcaccgcttt tttcactttc gccgggctcg atgtcttcgg
     4021 ccaaggcctt tctttctttt tccgtatcgt cgccaaaccc gagaatctgc ttcaccacca
     4081 aatccttgag gttcatcgtg ccgatccgga gaggccggtt ttgcgtttcc gtggtgaccg
     4141 gcggcgccgg gtcatcgccc accttttcct taaagccgat caagcgcata aagcgctcgc
     4201 ccacgctgcg cttgggtgga ggcgtgcgat cggccgttac cacggtattg gtggcttgct
     4261 cggcttccgc ttccggttcc ggctgggctt cggatttttc ctccgctttt gcctgctgca
     4321 cttcgtcttt cttgccaaag ccgaacaatc gcaaaaaacg ctcccccaag ccacgtttgg
     4381 gttgttccgt ttgagcttgt tgaacttgct gtgctgtcgc cggctttgcc tctatagctg
     4441 gaatggattg tggggactcg gcaagattgc tgtccagctt taactttaat ccctcgacgg
     4501 gcggcagcgg cggattcggt tgctccggcg cggctgcgac cgcggcaggc tgggtctggt
     4561 cggttttgtc gggaacaatc aattcgacag gccgggtgcc ggcgattttg tcgctcggcg
     4621 ggagcgccgc gccttcgatt tctttcagtt tgtcgccctc aaacaccgcc gtgagtttgc
     4681 ggctctccgt aagcttcccc gccttgttga agcggtaaac ataatcccaa cggttagcgt
     4741 ggaacgggtc ggaaatcagc ggcgtgccca gaacaaagcg cacttgggac ttggtcatcc
     4801 cgggcttgac cttgtccagc atgtcctgag tgaccacgtt gccctgctgc acgtcaatct
     4861 tgtgcggggt gatcagaacc gtgggcgagc aggccgtcag gaataaggcg aggaatatca
     4921 gccggtagcg catgaaaaaa ttgccgttta agccggagac gctatatgat accctagata
     4981 attgtcacac gtgagaagcg attatcatga gctcaccgag cgacctcaag accatcgggc
     5041 tcaaggccac gctgccgcgc ctcaaaatcc tcaatttgtt cgaaaacagc gaagtaaggc
     5101 atttgaccgc ggaagacgta tataaagttc tcaccggcga gggcatggac atcgggctcg
     5161 caacggttta tcgcgtgctt acccagttcg aacaagccgg gcttttgatt cgccatcact
     5221 ttgaaagcgg caaggcggtg tttgaattaa accagggcgg gcatcacgat catctggtgt
     5281 gcctgcaatg cgggcgggtg gaagagtttg ttgaccagga aatagagcgc cgccagatca
     5341 aaatcgccaa agagcgcggc ttcaccatcc acgagcactc gctgcacctc tacgccgact
     5401 gcaccaagcc gaactgcccc cataaaactt aggaacctct gaacaagtcc cccgtggagc
     5461 gcatctctgg cgcaatgcgg atgattgcta ggcgcacgac gaaggtgcct agccgtagct
     5521 taagcacgag gagcgcaacg acgccagcgc cgcggctacg aaaatgcgtt gaagttgctg
     5581 ccggcgcggg aatttgaagt attccgccgg cgggctgtcg ggcactcggt cgcggaaatt
     5641 tccgaaaccc tgaacctgaa ctggaacccg gtggccccag tccattgtca aacagaagct
     5701 tggcgtggaa agcagcgtac agagggcgcg gatcgcaata tcccatgcac tcagcggaaa
     5761 cgtgacgggg aactgaggcc ggtgacctgc attcggtccg cttactgcgc ttactttaga
     5821 gttcggtttt ctttcagtat cgtctcgatt ttggcgaacg gcaccggtgg gctgaacaag
     5881 aagccttgca tctcatcgca ctttagcagc ctgaggatct tggattgttc ttccgagtcc
     5941 acgccctcgg cgatcacctt gagattcagt gaatgcgcca gcgaaatgat cgtggatacg
     6001 atggtcatgc tgtccgggtc ctgcaccatg ttgacgatga acgcgcggtc gatcttcaag
     6061 gcgttgaccg gaagcttcgc caggtagccc agcgacgaat agccggttcc gaaatcgtca
     6121 atcgcaatgc cgacccccat gtccctcagc gccttgagct tgcggatatt gtcctcgatg
     6181 ttttccataa tcaggctttc ggtgatttcc aggtcgaggc tttgcgtgtc ggccgcatac
     6241 cctgcggtgg cctgtttcac cctgtcgaca aagtcttttt gtctcagctg gatcggcgac
     6301 acgttgaccg cgatgcgcgg gtgcagccct ttgacggccc agcgctggca gtccaccaaa
     6361 gcctggcgga tgacccaata acccacatcg agtatcaggc cggtttcttc gagaaggggg
     6421 ataaacttga gcggcggcac caaccccgtt tccgggtcct gccagcggat caacgcttcc
     6481 aggccgctca cctgccggct tacaaggtcg attttcggct ggtagtgaag cacgaactgc
     6541 tgctgttcca aagccacgcg cagtttgttc tccagagcca gtttttcggt gacgcgggcg
     6601 ttcatttccg gagcataaaa aagggatcgc tcgcccgagg tcttggccca gcccaatgcg
     6661 gcttccgcgt tcctgaacag cgtttcggca ttgtccccgt catgcgggga caatgccacg
     6721 ccaactttca cggcgatgcg gagctcctgg ttgcctaatt tgaaaggctg attcagtggc
     6781 tggaagattt tctgctcaat ggcatcaaca atccccgctt cttcgcgcac gttggttatc
     6841 atggccacaa agcagtctgc cgcaagacgc cccgggctgg cagcgcttcc gaatgaacgg
     6901 gtcagccgct cccccacctg cttgagcaac gcatccccac cctgccggga caggctctcg
     6961 ttaatttccc gaaagcgctc gatgtccacg aaagccactg ccgtgcgggt tccttccctg
     7021 cgcgccgcct ggaggttttg attgaggcgg tcgtagcaga gcgcacggtt gggaagaccg
     7081 gttaccatgt cgtagtaggc gaggtaatta agcttttctt cttggagctt gcgctcggtg
     7141 atgtcccgca tgaaaccggc aaacaaaggt ttcccgccaa tatccatatg gctgaaagag
     7201 atttccatgg ggaactcgtg gccgtcccgg tgcaacgcaa cgacttcagt gg
//
LOCUS       DATIOW010000150        13589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_169498, whole genome shotgun sequence.
ACCESSION   DATIOW010000150 DATIOW010000000
VERSION     DATIOW010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13589
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169498"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VLL03_04990"
     CDS             complement(<1..1043)
                     /locus_tag="VLL03_04990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSS46751.1"
                     /translation="MGIPLRVLIVEDSEANASQLLRELRSGGYDPISERVDTAATMQA
                     MLRRQEWDCVISDISMPSFSATAALELLKTSGIDLPFILVLGTVGEHTAVATMRAGTR
                     DLMIKQNLTRLIPVVERELHEATVRREQKRIEKSILESEERYRMLVETASDGIFIQCD
                     GKFIFVNSAGLGILGATSAEQVTNKPVYDFIHPEFREIAREHIRQLEEEGKKVPLHEE
                     KILRHDGSTVEVEVTAVPFVFQGKPAAHLIMRDITDRKITERRLAWEHAMTRVLAESP
                     TLSQAASEEKLSGILGSIDNVIWSISASTYETLYLNPAAEKLYGMPVTEFYKNKDLWL
                     SMVHPEDRKRVGE"
     gene            complement(1624..2616)
                     /locus_tag="VLL03_04995"
     CDS             complement(1624..2616)
                     /locus_tag="VLL03_04995"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSS46752.1"
                     /translation="MQHRKLGPRGLMVSALGLGCMGMSDFYGERDEAESIATIHRALE
                     LGINFLDTAEIYGPFANEELVGRAIKGKRNKVIIATKFGIVRKAGDPNYWALDSRPER
                     VRTSCEGSLKRLGVDCIDLYYQHRLDPKVPIEETVGAMAKLVREGKIRYLGLSEAGLE
                     TIRRAHAVHPITALQSEYSLWSREPEDDEVLQTVRKLGIGFVAYSPLGRGFLTGQIRS
                     PEDLSPDDWRRTHPRFQGENFKKNLELVERLKEIAKAKSATPAQIALAWVLSSGDDVV
                     PIPGTKRRTRLEENAAAVTIELSPEERTRLDTVFRLGVAAGARYGPVAAARINR"
     gene            2880..4712
                     /locus_tag="VLL03_05000"
     CDS             2880..4712
                     /locus_tag="VLL03_05000"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HSS46753.1"
                     /translation="MNQFNATAVETANAPAYVKNKRLKEWVAQMAVLCKPVRIYWCDG
                     SQEEYNRLCNEMVASGTFIKLNPELRPDSYLALSDPSDVARVEDRTFICSRNKDDAGP
                     NNNWIVPDEMRATLNKLFDGCMRGRTMYVVPFSMGPLGSRIAHIGVEISDSPYVAVNM
                     RIMTRMGRKVLDVLGADSEFVPCMHSVGMPLGPGQKDARWPCNKEHKYIVHYPETREI
                     WSYGSGYGGNALLGKKCFALRIASIMGRDQGWLAEHMLILGVEPPQGQKLYVAAAFPS
                     ACGKTNFAMLIPPSNFKGWKVTTVGDDIAWIKPGKDGCFYAINPEAGYFGVAPGTSEQ
                     SNPNAMEMLKRNCIFTNVALTADGDVWWEGMSKIPPAKLTDWQGKEWTPDGGRPAAHP
                     NSRFTVSSTQCPSLDPAWDDPNGVPISAFIFGGRRSTAVPLVYEARSWDEGVYLAATM
                     GSETTAAATAQQGVVRRDPFAMLPFCGYNMADYFKHWLKMGRVIAHPPKIFGVNWFRK
                     GPDGKFIWPGFGENMRVLKWIVERCLGRAHAAGSPLGWVPDYKDLEWKGLEEISADKF
                     HALMSLDGEVWKQELASHSELFDKLKERLPKELATRRERLQSSF"
     gene            4718..5476
                     /locus_tag="VLL03_05005"
     CDS             4718..5476
                     /locus_tag="VLL03_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HSS46754.1"
                     /translation="MDLAFLGAAAFVAGLLDAVVGGGGLVQIPALFSVMPNGAPATVF
                     GTNKLSSIFGTGIAGLRYARAISMRWNAVLPAMATALVFAYIGAYSVAQIPSHVLRPL
                     VPFMLIAVAVYIFVKKDFGAVHAPRHEGLREKILGLTLGGVIGFYDGFFGPGTGSFLM
                     FLFIRFFGLDFLSASAAAKFVNVACNAAALAYFWPGGHVIWQLGLTMAVCNVLGSLIG
                     SRLALRYGSQFVRRLFLLVVSAMILKLSYDLFLK"
     gene            5524..7419
                     /gene="mnmG"
                     /locus_tag="VLL03_05010"
     CDS             5524..7419
                     /gene="mnmG"
                     /locus_tag="VLL03_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HSS46755.1"
                     /translation="MQFPTEFDVIVVGGGHAGTEAALACARLGQKTLLLTHNIETLGQ
                     MSCNPSIGGIGKGHLVKEIDALGGAMALATDEAGIQFRILNSSKGPAVRATRAQADRV
                     LYKQAIRSRLENQRDLWLFQQAVDDLTLEGDRVTGVVTQLGLRFSGRAVVLTTGTFLS
                     GLIHVGLSNYQAGRAGDPPALSLAARLRELKLPVGRLKTGTPPRLDGRTIDYSVMEIQ
                     AGDNPVPVFSFLGRPEMHPRQLPCWITHTNSRTHEIIRGGLDRSPMFTGVIEGIGPRY
                     CPSIEDKIHRFASKQSHQIFLEPEGLNTNEVYPNGISTSLPFDVQLALVRSIKGLENA
                     HILRPGYAIEYDYFDPRGLKSSLETKAIKNLFFAGQINGTTGYEEAAAQGLLAGINAG
                     LLVQGKEPWCPRRDEAYLGVLVDDLITRGLSEPYRMFTSRAEYRLQLREDNADLRLTE
                     IGRRHGVVDNARWEQFEKKREEVAREHERLKNTWVNPKLSPQSEAERVLGAAIEREYT
                     LEELLRRPNVTYDSLMTLPGAGPAVQDEKVAEQVEIQTKYRGYIERQRDEVARRENYE
                     NTRLSQDLDYRAVRGLSVEAQQKLNQHKPETLGQAARISGVTPAAISLLLVYLKRGCN
                     AKHKKTA"
     gene            7416..8039
                     /gene="rsmG"
                     /locus_tag="VLL03_05015"
     CDS             7416..8039
                     /gene="rsmG"
                     /locus_tag="VLL03_05015"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HSS46756.1"
                     /translation="MSLAGELRQGVTALGLKLSPEVQQRLLNYLALLNKWNRVYNLTA
                     VREPQKMVSHHLLDSLAVVPHIEAQTIVDVGSGAGLPGIPLAIAREEWQVTLLDGNHK
                     KAAFLRQAAIELKLANVAVRCERAEDFQPQEKFDCVISRALSDLPEYVELAGHLCKNT
                     GTVVAMKGVYPNEELAQLPRKFKMQQVIPLKVPGLKAERHLVIMKAA"
     gene            8040..8822
                     /locus_tag="VLL03_05020"
     CDS             8040..8822
                     /locus_tag="VLL03_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HSS46757.1"
                     /translation="MPKILAVTNQKGGVGKTTTSVNLAASLAAENCRVLLIDLDPQGN
                     ATMGSGIDKHSLQRTVYQVLLGSATLGEVKIASQSGGFDLIPANRELAGAEVELVEVP
                     DRENRLKAALEEIQSNYDFILIDCPPALNLLTVNGLCAAQAVMIPMQCEYYALEGLSD
                     LVSTVKKIRANLNPALQIEGLLRTMFDPRNLLAQQVSDQLKQHFGDKVYDTIIPRNIR
                     LAEAPSHGVPAIHYDRTSKGAQAYLALAGELLSRSGQLPTAA"
     gene            8835..9680
                     /locus_tag="VLL03_05025"
     CDS             8835..9680
                     /locus_tag="VLL03_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HSS46758.1"
                     /translation="MKIKGLGRGLDALLAGNDEAPHKGEQLATLPIDQIQPGRYQPRT
                     RMDAESLAELAESIKSQGVMQPILVRPLPKGRYEMIAGERRWRAAQMAGLSQVPALVR
                     QVPDEAALAMSLIENIQREDLNPLEEAGGIQRLISEFKMTHQSAAEAIGRSRSGVTNL
                     LRLINLAAPVQELLMAGRIDMGHARALLGLSAAKQIELANLVAHKGMSVRQIEQLVRQ
                     REKTTHKKTEKPDRDLIRLQEELSEKLGATVSIKPGKRGTGRLIINYASLDELDNLIS
                     KLCKH"
     gene            9718..9969
                     /locus_tag="VLL03_05030"
     CDS             9718..9969
                     /locus_tag="VLL03_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46759.1"
                     /translation="MKAFKFFSAIFGAILTFGCGQGEQKDTAAKAENPSRQKVKDPEK
                     LPGQRLQQAGQAMNKAADVEKELAKKAEETERKIEQESK"
     gene            10274..10621
                     /locus_tag="VLL03_05035"
     CDS             10274..10621
                     /locus_tag="VLL03_05035"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HSS46760.1"
                     /translation="MLSQIALTLLTAAAAFALRGSAAALAALYGGGVALSGTWLLGRR
                     VARTPETAQQGDVGSQLALYAGVLPRFIVTLALLAVGIGGLKLAPIPLIIAFGLAQLG
                     FVINLGSARPPPE"
     gene            10635..11417
                     /gene="atpB"
                     /locus_tag="VLL03_05040"
     CDS             10635..11417
                     /gene="atpB"
                     /locus_tag="VLL03_05040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HSS46761.1"
                     /translation="MSEAGKPNAVEYIQHHLTNLQVGQGFWTFNLDTLVFGWVLAGFL
                     VFLSWRMGRSLSLDNPRGAQNVLEAIMEFVDSQIKSAFPAPDPLIGPLALTVFLWVFL
                     MNAMDLIPVDLLPKAAEYLGVHHLKVVPTTDPHTTLGMSLSVFALILYYNIKIKGAWG
                     YFKSFLFHPFGKWLMPVNFVMTAAEELAKPLSMGLRLFGNMFAGELVFLLIALLPWWI
                     LWVPGTLWAIFHILIITLQAFIFMILTIMYLALTYQSLDDAH"
     gene            11460..11762
                     /gene="atpE"
                     /locus_tag="VLL03_05045"
     CDS             11460..11762
                     /gene="atpE"
                     /locus_tag="VLL03_05045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF005363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HSS46762.1"
                     /translation="MEAGMVNLVGMIYAYTAVGVGIILAAAGLGSALGWGLICAKYME
                     GIARQPEMLPVLRIQMFITAGLMESFPFIVLAFAMYFTFANPFLGAALNALKAAGG"
     gene            11817..12287
                     /locus_tag="VLL03_05050"
     CDS             11817..12287
                     /locus_tag="VLL03_05050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HSS46763.1"
                     /translation="MNVTATLFGQMITFAVLVWFIQHFLWGPLTRMLAERNKRIADGL
                     AAAERGKQELEIAGKRSAEVLQDGKQQAADIIAQAEKRAQQIIEEAKHAAKAEGDRMI
                     AGAKAEIVQEVSRAKEALRSQVAALAVAGAEKILRREVDAKAHTDLLKVIQTEL"
     gene            12294..12830
                     /locus_tag="VLL03_05055"
     CDS             12294..12830
                     /locus_tag="VLL03_05055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HSS46764.1"
                     /translation="MAENVTIARPYAEAVFKLAREKNTLAKWSDMLAYLEVVVTESRV
                     QAIIGNPNFSAEQTENLLLGICGEKLDGAGRNFVQVLERNDRLLLLPEIRELFEALKA
                     EQEGVLEVKIDSAFPLSKQQAGALVRRLEGRYQRKIVTQVSVEPELIGGVKIAVGDHV
                     LDATVRGKLEAMRAALTR"
     gene            12865..>13589
                     /locus_tag="VLL03_05060"
     CDS             12865..>13589
                     /locus_tag="VLL03_05060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524507.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HSS46765.1"
                     /translation="MQVNPSEISELIKSKIENLAVSAESRTQGTVISVTDGICRVHGL
                     SDAMQGEMLEFPKNTFGLALNLERDSVGSVVLGEYEHITEGDTVKCTGRILEVPVGEA
                     LIGRVVNSLGQPIDGKGPIQAKETSPIEKIAPGVIWRKSVSQPVQTGLKSIDSMVPIG
                     RGQRELIIGDRQTGKTAVALDTIINQQGKDLICIYVAIGQKASSIVNVVRKLEEHGAM
                     AYTIVVAASASESAAMQYIAPYSG"
BASE COUNT         2926 a         3787 c         4031 g         2845 t
ORIGIN      
        1 atctccccaa cgcgcttgcg gtcttcggga tgcaccatac tgagccagag gtccttgttc
       61 ttgtaaaact ccgttaccgg catcccgtac agtttttctg ccgcagggtt aaggtagagg
      121 gtctcataag tcgacgccga aatggaccag atgacattgt cgatggatcc taggatgccg
      181 ctcagctttt cttctgaggc ggcttggcta agcgtagggg attccgccaa gactcgggtc
      241 atcgcatgct cccacgccaa gcgcctttcc gtaatcttgc ggtcggtgat atctcgcatg
      301 attagatggg ctgccggttt gccctgaaag acgaaaggta ccgccgtcac ctctacctct
      361 accgttgatc cgtcatgccg gagaatcttt tcttcatgaa gcggtacttt ttttccttcc
      421 tcctcaagct ggcggatgtg ctccctggct atctctcgaa attcagggtg gatgaagtca
      481 tacaccggct tatttgtaac ttgttccgcg ctggtggcac ctaaaatccc tagcccggcg
      541 ctattcacaa agatgaattt gccgtcgcac tgaatgaaaa ttccgtctga tgctgtttcc
      601 accagcatgc ggtagcgctc ttcgctttct aatatggatt tctctatacg cttctgctcc
      661 cggcgtacgg tggcttcgtg gagttcacgc tcgactacag ggattaaccg agtgagattt
      721 tgcttgatca tcaaatcgcg ggtcccggcc ctcatcgttg ccacagcggt atgttcgccc
      781 actgtaccca agacgaggat aaaaggcagg tcaatcccgc tggttttaag cagttccaga
      841 gcagcagtcg cgctgaagga cggcatggaa atgtcggaaa tcacacaatc ccattcctgc
      901 ctacgaagca tcgcttgcat cgtcgcagca gtgtccaccc tttcagatat tgggtcgtaa
      961 ccgccactgc gcaattccct taatagctgc gacgcgttgg cttccgagtc ctcgacgatc
     1021 aaaacgcgca gtgggatacc cattgactat gctatgacgt gactgaagtt ttttagatga
     1081 cgtgactaac aattttttag agtaagttgt ttagagacac tttagcggtt tgtcgccaca
     1141 tcgtaaagct cattttagtc gtgttcacca ggcgggtatt gtgattctcg cgccagcgct
     1201 cgaggaggat ccgggcttcc ttcaatctat agaagacctt accgttcaac ggtaacctgc
     1261 ccggcttttt ctgtaccagg ttgatcgaga gcgttgcaac taacgaaggc gtgaccgctt
     1321 cagttaagct tgctccttga tccttcaagg aaacccgagc cggattactg gtggcaagtc
     1381 acaggagtcg cacgtcggcg acacaaaggc cacgaaaacc gcccgcgtcc aattcttaac
     1441 caggattgtg tccaagtttg tgtccaaatg ctcctgcgag caagctcaaa atcccgagag
     1501 aataaatact tgcgcttgct ttctagattt tgcataggaa aataagctcg cttccattcg
     1561 tggggccagg gaagcaaatt cgctagcggt caaccggctt gcttcggagt gggtcgcctt
     1621 cggtcaacgg ttgatccgcg ccgccgcgac gggcccgtag cgcgcgccgg ccgccacgcc
     1681 aagacggaac acggtgtcga ggcgggtccg ttcctcgggc gagagttcaa tcgtgacagc
     1741 ggcagcgttc tcctccaacc gggtacggcg cttggtgcct ggaatgggta cgacgtcatc
     1801 tccacttgat agcacccagg cgagggcaat ctgagcgggc gttgcgcttt tcgccttggc
     1861 aatctccttc aggcgctcca ccagttcgag gttcttcttg aagttctcgc cttggaaccg
     1921 cggatgggtg cgacgccagt catctggaga caagtcttcg gggctgcgga tctgcccggt
     1981 gaggaaaccg cggcccagag gcgagtaggc gacgaaaccg atcccgagct tgcgcacggt
     2041 ctggaggacc tcatcgtctt ccggctcccg actccagagt gagtattcgc tctgcagcgc
     2101 tgttatcggg tgtaccgcat gcgcgcgccg gatagtctcg agccccgctt cggagagccc
     2161 gagatatcgg atttttcctt cccggacgag ctttgccatt gcgcccaccg tctcctcgat
     2221 cggcaccttg gggtcaaggc gatgctggta gtaaagatcg atacagtcca cgccgaggcg
     2281 cttcagcgag ccttcgcacg aggtgcggac gcgctcggga cggctgtcca gcgcccagta
     2341 gttcggatcg cctgccttgc gcacaatgcc gaacttggtg gcgatgataa ctttattgcg
     2401 tttgcccttg atcgcacgcc ccaccagttc ctcgttggca aaaggcccgt agatctccgc
     2461 tgtgtcgagg aaattaatgc cgagttcaag cgcacggtgg atcgtcgcga tcgactccgc
     2521 ctcgtcgcgc tcgccgtaga agtcgctcat ccccatacac ccgaggccaa gggccgagac
     2581 catgagaccc cgggggccaa gcttgcggtg ttgcatacgc aaatctcctt ccgttcgtta
     2641 tttccagcgc aggaacactt acatgcgcct tgcggctttt gcattgcttt gggccgattg
     2701 gcgcggttgg caaggagcgg tgggtttaat tataatggtt taactgtaag ggcttcctcg
     2761 cgctgccttg agttccttcg gggcggtcgt catccgtttc cattttgaag aggttccgga
     2821 ctgccggggg agggggattc tttgacattc caaataaccc gaagggatga ggtaaaaaca
     2881 tgaatcagtt caatgccacg gcagttgaaa cggccaatgc cccggcctat gtgaagaaca
     2941 agcggcttaa ggaatgggtc gcgcaaatgg cggtgctgtg caagccggtg cgtatctact
     3001 ggtgcgacgg ctcgcaagaa gaatataacc ggctgtgcaa tgaaatggta gcctccggca
     3061 cgttcatcaa actcaacccc gagcttcgcc ccgacagtta ccttgccctt tccgacccca
     3121 gcgacgtggc gcgcgtggaa gaccgcactt tcatctgcag caggaacaaa gacgacgccg
     3181 ggcccaacaa caactggatt gtgccggacg aaatgcgtgc tacgctgaat aagcttttcg
     3241 acggctgcat gcgcggccgc accatgtacg tggttccgtt ttccatgggc cccttgggtt
     3301 cgcgcatcgc acatatcggc gtggaaatca gcgattcacc ctatgtcgcg gtcaacatgc
     3361 gcatcatgac gcgcatgggg cgcaaggtgc tcgacgtgct gggtgccgac agcgagttcg
     3421 tgccgtgcat gcactcggtg ggcatgccgc ttggccccgg acagaaagac gcgcgctggc
     3481 cgtgcaacaa ggagcacaaa tacattgtgc attacccgga aacgcgcgaa atctggtctt
     3541 acggcagcgg ctacggcggc aatgcgctac tcggcaagaa atgcttcgcg ctgcgcattg
     3601 cctccatcat gggccgggac cagggctggc tggccgagca catgctgatt ctcggcgtgg
     3661 agccacccca ggggcaaaaa ctttacgtgg ctgcggcttt ccccagcgct tgcggcaaga
     3721 ccaattttgc catgctgatt ccgccgtcga acttcaaagg ctggaaagtg actaccgtag
     3781 gcgacgacat cgcctggatc aagccgggca aggacggatg cttttacgcc atcaacccgg
     3841 aagcgggcta tttcggcgtc gcacccggca cctccgagca gtcgaacccc aatgccatgg
     3901 agatgctcaa gcgaaactgt attttcacca atgtcgcgct cactgccgat ggcgatgtat
     3961 ggtgggaggg catgagcaag attccgccgg ccaagcttac cgactggcag ggcaaggaat
     4021 ggacgccgga tggcggccgc cccgctgcgc accccaattc gcgctttacc gtgtcttcga
     4081 cccaatgccc ttcgctcgat ccagcctggg atgatccgaa cggcgtgccg atcagcgcct
     4141 tcattttcgg cgggcgccgc agcaccgccg tgccgctggt gtacgaggcg cgctcctggg
     4201 atgagggcgt gtatctcgcc gccaccatgg gctcggaaac caccgctgct gcgactgcgc
     4261 agcagggcgt ggtcaggcgc gaccccttcg ccatgctgcc gttctgcggc tacaacatgg
     4321 cggattattt caagcactgg cttaagatgg gccgcgtcat tgcccatccg ccgaagattt
     4381 ttggcgtcaa ctggttccgc aaagggccgg acggcaaatt catctggccc ggtttcggcg
     4441 agaacatgcg ggtgctgaag tggatcgtcg agcgctgtct ggggcgtgct cacgccgcag
     4501 gaagcccgct gggttgggtg ccggattaca aagacctgga atggaaaggg ctggaagaaa
     4561 tttccgcgga caagttccac gcgctcatgt cgctcgatgg ggaagtgtgg aagcaggaac
     4621 tcgcttcgca cagcgaactc ttcgacaagc tcaaggagcg cttgcccaaa gaattggcga
     4681 ctcggcgcga gcgtttgcag tcgtcgtttt aaggtaattg gatctcgctt ttcttggtgc
     4741 agcagctttt gtcgccgggc tgcttgatgc ggtagtcggc ggcggcgggc tggtgcaaat
     4801 cccggcgctg ttcagcgtga tgccgaacgg cgcgcccgcc acagtattcg gcaccaacaa
     4861 gctctcctcg attttcggta ccggcattgc gggcttgcgt tacgcccgcg ccatttccat
     4921 gcgctggaat gcggtgctgc cggcgatggc tacagccctg gtttttgcct atatcggcgc
     4981 ctattccgtc gcccaaatcc cttctcatgt actgcgcccg ttggtaccgt tcatgctgat
     5041 cgcggtcgcc gtctatatct tcgtcaaaaa ggattttggc gcggttcatg cgccacggca
     5101 tgaaggattg agagaaaaaa tcctgggctt gacgctcggc ggcgtgattg gcttttatga
     5161 cggcttcttc ggcccgggta ccggcagttt cctgatgttt ttgttcatcc gctttttcgg
     5221 cctggatttc ttgagcgcgt ccgccgccgc caagttcgtc aacgtcgcct gcaatgcggc
     5281 cgcgctcgct tatttctggc ccggcggcca cgtgatttgg cagctcggcc tcacgatggc
     5341 ggtctgcaac gtgctgggct ccctcatcgg ctcgcggctt gccttgcgct atggcagcca
     5401 atttgtgcgg cgattgtttc tgttggtagt tagcgcgatg atactcaaat tgagctacga
     5461 cctttttctc aagtagcctg atttggcgta ttattacgcc tctttggagc ttcccgtaat
     5521 tccatgcagt ttccaaccga atttgacgtg attgtggtag gcggtggcca cgccggcacc
     5581 gaggccgcgc tcgcctgcgc gcgcctgggg caaaaaaccc tgcttctcac ccacaacatc
     5641 gaaacgctgg ggcagatgtc gtgcaacccg tccatcggcg gcatcggcaa agggcacttg
     5701 gtcaaggaaa tcgatgcgct gggcggcgcg atggcgctag ctactgacga agcgggaatc
     5761 cagttccgca ttctcaattc cagcaaagga ccggcggtgc gcgccacccg cgcccaggcc
     5821 gaccgcgtgc tttacaagca ggcaatacgc agccgtttgg aaaaccagcg tgatctctgg
     5881 ctgtttcaac aagcggttga tgatttgact ctggaaggcg atcgcgtcac cggcgttgtc
     5941 acccagcttg ggctgcgctt ttccggccgc gccgtggtgc tcactaccgg cacattcttg
     6001 tccggactga ttcatgtggg cttatcgaat taccaggcgg gacgcgcggg cgatccgccc
     6061 gccctcagcc tggcggcgcg gctgcgcgaa ttgaagctgc cggtaggccg gctcaaaacc
     6121 ggcacgccgc cgcgcctgga cgggcgcacg attgattact cggtgatgga aattcaagcg
     6181 ggcgataacc cggtaccggt attttcgttt ctcggcaggc cggaaatgca cccgcggcaa
     6241 ttgccgtgct ggatcaccca taccaattca cgcacccacg aaatcatccg tggcgggctc
     6301 gaccgctcgc cgatgttcac cggcgtcatc gaaggcatcg gcccgcgtta ttgcccttcg
     6361 attgaggaca agattcaccg ctttgcgagc aaacaatcgc accagatttt cctcgagccg
     6421 gaaggcttga acaccaacga agtttatccg aacgggattt cgacgagcct cccgttcgat
     6481 gtgcagctgg cgctggtgcg ctcgatcaag ggactggaaa atgcccatat cctacgcccg
     6541 ggttacgcca tcgaatacga ttatttcgat ccgcgcgggc tgaaaagctc gctggaaacc
     6601 aaggcgatca aaaatttgtt ttttgccgga caaatcaacg gcaccaccgg ctacgaggaa
     6661 gcggcggcgc aaggtctgct cgccggaatc aacgcggggc ttttggtgca aggcaaggaa
     6721 ccctggtgtc cgcgccgcga cgaggcctac ctcggcgtgc tggtggatga cctgattaca
     6781 cgcggccttt ccgagcccta ccgcatgttt accagccgcg ccgaataccg cctgcagctg
     6841 cgcgaggaca atgccgacct gcggctcacc gaaatcggac ggcgtcacgg cgtggtggat
     6901 aatgcgcgct gggagcaatt cgagaaaaaa cgtgaagagg tggctcgcga acatgagcgg
     6961 ctgaaaaaca cctgggttaa tccgaaactt tcgccgcaaa gcgaagcgga gcgggtgctt
     7021 ggcgcagcga tagagcgcga atatacgctc gaagaattgc tgcgacggcc caatgtgacc
     7081 tatgactcgc tcatgacttt gcccggggcg gggcctgccg tgcaggacga aaaagtcgcg
     7141 gagcaggtgg aaatccagac caaataccgc ggctatatcg agcgccagcg cgacgaagtc
     7201 gcgcgccgcg aaaattacga gaacacgcgc ctgtcgcaag acctagatta ccgcgccgtg
     7261 cgcggccttt cggttgaagc gcagcaaaag ctcaaccagc acaagccgga aactttgggt
     7321 caagcggcgc gaatttccgg agtgacgccc gcggcaatct cgctgttgct cgtgtatttg
     7381 aagcgcggtt gtaacgccaa gcataaaaaa accgcatgag cctggcgggc gagctcagac
     7441 aaggcgttac cgccttgggg cttaagctgt cgccagaagt acagcagcgg ttgttgaatt
     7501 acctcgcgct gctcaacaaa tggaacaggg tgtacaacct caccgcggtg cgcgagccgc
     7561 agaaaatggt gagccaccac ttactcgata gtcttgccgt ggtaccgcat attgaagcgc
     7621 aaaccatcgt ggatgtgggg agcggcgcgg gattgccggg gattcccctg gcgattgcgc
     7681 gcgaggagtg gcaggtaacg ctgcttgacg gcaaccacaa gaaagccgcg tttctcaggc
     7741 aggcggcgat tgaattgaag ctcgccaatg tcgcggtgcg ctgcgagcgc gcggaagact
     7801 ttcagccgca ggaaaaattc gattgcgtga tttcgcgcgc gctttccgat ctcccggagt
     7861 atgtggagct tgctggacat ttgtgcaaga acactggaac cgtggtcgcg atgaaaggcg
     7921 tttatcctaa cgaagagctc gcccagttgc cgcggaaatt caaaatgcag caagtgatac
     7981 cgctcaaagt gccggggctt aaagccgagc ggcacctggt catcatgaaa gcggcttgaa
     8041 tgccgaaaat tcttgcggtc accaatcaaa aaggcggagt ggggaaaacc accaccagcg
     8101 tgaatcttgc agcgagcctg gctgcggaaa actgtcgagt gctgctgatt gacctcgatc
     8161 cccagggcaa cgccaccatg ggcagcggca tcgacaagca cagcctgcag cgcacggttt
     8221 atcaagtgct gctcggaagc gcgaccctgg gcgaggtcaa gattgcatca caaagcggcg
     8281 ggtttgattt gattcccgcc aatcgcgagc tggccggcgc ggaagtggaa ttggtggaag
     8341 tgcccgacag ggaaaaccgc ttgaaagcag cgcttgagga aattcaaagc aactacgatt
     8401 tcatccttat agattgtccg ccggcgctca acctgcttac ggtgaacggc ttgtgcgccg
     8461 cgcaagcggt gatgataccg atgcagtgcg aatactacgc gctggagggt ttgagtgatc
     8521 tggtctcgac ggttaaaaaa atacgcgcca accttaaccc cgcgctgcaa atcgagggct
     8581 tgttgcgcac catgttcgat ccgcgcaatc ttctggcgca acaggtttcc gaccaactga
     8641 agcagcactt cggagacaag gtttacgata ccattattcc gcgcaacatc cgtctggccg
     8701 aggcgccgag ccacggcgtg cccgcaatcc attacgatag aacctccaag ggcgcgcagg
     8761 cttatctggc gctggcggga gaactattaa gccgcagcgg ccagctgccg accgcggcgt
     8821 gacagggaag aaagatgaag attaaaggtt tggggcgggg tttggatgcg ctcctcgcag
     8881 gaaacgacga ggcgccgcac aaaggcgagc agctcgccac gctcccgata gaccagatcc
     8941 agcccggcag atatcagccg cgcacgcgca tggacgcgga atcgctggcg gaactggccg
     9001 aatccatcaa gtcccagggc gtgatgcagc ccattctggt gcggccgctg ccgaaggggc
     9061 gttatgaaat gatcgccggc gagcgccgct ggcgcgccgc gcagatggcg ggtttgagcc
     9121 aggttcccgc gctggtaaga caggtgcccg atgaggcggc actggcgatg tcgctgattg
     9181 aaaacatcca gcgcgaagac ttgaatcctc tggaagaagc cggtggcatc cagcggctga
     9241 ttagcgaatt caagatgacc catcaaagcg ccgccgaagc gatcggccgc tcaagaagcg
     9301 gcgtcaccaa tttgctcagg ctcatcaatc tcgccgcccc cgtgcaggaa ttgctgatgg
     9361 caggcaggat tgacatgggt cacgcgaggg cgctcctcgg tctgagcgcg gcgaagcaaa
     9421 ttgaactggc caatctggtc gcacacaaag ggatgtcggt gcggcaaatc gaacaactcg
     9481 tcaggcagcg ggaaaaaacc actcacaaga aaaccgaaaa acccgaccgc gaccttatca
     9541 ggctgcaaga agagttgtcg gagaaattag gcgccacggt aagcataaaa ccgggcaagc
     9601 gcggcacggg aagactcatc atcaattacg ctagcctcga cgagctggac aacctcatca
     9661 gcaaattgtg caagcattga tatttgtgcg tgatgcgcca acaataggaa gcgcgctatg
     9721 aaagccttta agtttttttc cgcaattttt ggcgccatcc taaccttcgg ttgtggccaa
     9781 ggagagcaaa aagatacggc cgcgaaagca gaaaatcctt cccggcaaaa ggttaaagat
     9841 ccggaaaaac ttcccgggca gcgcctgcag caagccgggc aggccatgaa caaagccgcc
     9901 gatgtagaaa aggagctcgc gaaaaaagcg gaagaaaccg agcgcaaaat tgagcaggaa
     9961 agcaaatagc cggttatagc ccgctttcgc tggccacgaa tatcaagcgg ccacctccga
    10021 ctgggctggg taggggtcaa tttgatggaa ccaggtattt ccggccatgt ccggagtttg
    10081 gatgaaatca cggctttggc ggtgtgaaag cgcactaaac caagcgggct cgtcgcttga
    10141 ccgctgaatg ccgcggcagt taaacttgca aatttgcgcg atgctgcagc tgtctactta
    10201 atccttcgag tcggccgtaa aaatcacaat gagtcatgct gcgggaattc tgagtcaggg
    10261 catccgtaga gtattgctca gccaaatcgc actgactttg ttgactgcgg cggcggcgtt
    10321 tgcgttgcga gggagcgctg cggcgctagc ggcgctttat ggcggagggg ttgctttaag
    10381 cggcacttgg ctgctggggc ggcgtgttgc gcgcaccccc gaaaccgcgc aacaaggcga
    10441 tgtgggcagt caattggcgc tgtacgccgg ggtgttgccc aggttcatcg tgacgctggc
    10501 actgctggca gtcggaatag gcgggctcaa acttgcgccc attcccttga ttatcgcgtt
    10561 tggtcttgcc caactgggtt ttgtgattaa tctgggaagc gcccggccgc cgccggaatg
    10621 aaacaggtaa aaaaatgagc gaagcgggca agcccaatgc agttgaatac attcaacatc
    10681 atttaaccaa tctgcaggtt gggcagggat tctggacctt taatctcgac acgctggtgt
    10741 tcggctgggt gcttgccggt tttcttgtct ttctgagttg gcgcatggga cgcagcctga
    10801 gcctcgataa tccgcggggg gcacagaacg tattggaagc aattatggaa ttcgtggata
    10861 gtcaaataaa atccgctttc cctgcacccg atcccttgat tggcccgctt gcacttaccg
    10921 tattcctatg ggtatttctg atgaacgcca tggatttgat tccggtggac ctgttgccca
    10981 aggccgccga atacctggga gttcatcatc tcaaggtggt gcccactacc gatcctcaca
    11041 ccacgctggg aatgtcgctt tccgtgtttg cgctcattct gtattacaac atcaaaatca
    11101 aaggcgcttg gggttatttc aagagttttc tgtttcatcc attcggaaaa tggctgatgc
    11161 cggtcaattt cgtaatgacc gccgccgaag aactcgccaa gcccctgagt atgggtctgc
    11221 gcttattcgg caatatgttc gcgggcgaac tggtgtttct gctgattgcg ctccttccct
    11281 ggtggatact ctgggtgccc ggtacgctgt gggcgatttt tcatattctg atcatcaccc
    11341 tccaggcttt cattttcatg attttgacca tcatgtattt ggcgctgacg taccaaagct
    11401 tggatgacgc acattgaggg gaataaagac ttcgctgaat ttttttaggg agagtaatta
    11461 tggaagcagg catggttaat ctggtcggca tgatttacgc ctacaccgcg gtgggtgtgg
    11521 gcattatcct cgctgccgca ggattgggtt ccgcgttggg ctggggcctc atctgcgcga
    11581 aatacatgga ggggatcgcg cggcagccgg aaatgctgcc ggtgttgcgc atacagatgt
    11641 tcatcaccgc gggccttatg gagtcattcc cgttcattgt gttggccttt gccatgtatt
    11701 tcacgttcgc taacccgttc ttaggggccg cgctgaacgc gctcaaggcg gccggaggct
    11761 aacatgacag gagttccgcg agcaggcgcg gagcaaacga aaaaaggagt ttagacgtga
    11821 acgtaacggc aacgcttttt ggtcagatga tcacctttgc ggtgctggtt tggtttattc
    11881 agcactttct ttgggggcct ttgacccgca tgctggcgga acgcaacaag cgcatcgccg
    11941 acggccttgc cgccgcggaa cgcggcaagc aggaactgga aattgccggc aagcgctctg
    12001 cggaagtgct gcaggacggc aagcagcagg ctgccgacat catcgcccag gcggagaaac
    12061 gcgcccagca aatcatcgag gaagcgaagc atgcagccaa ggccgagggc gaccgcatga
    12121 tcgctggcgc caaggcggaa atcgtgcagg aagtgagccg cgccaaggaa gcactgcgca
    12181 gccaggtggc ggctttggcg gtggcgggcg ccgagaaaat tctgcgccgt gaggtggacg
    12241 ccaaggcgca caccgattta ctgaaagtca tccagaccga actgtaaaac gccatggcgg
    12301 aaaacgtcac aatcgcgcgg ccttacgctg aagccgtatt caagctggcc agggaaaaaa
    12361 acaccctggc caaatggtcg gacatgctcg cgtacctgga agtggttgtg acagaatcgc
    12421 gggtacaagc catcatcggc aacccgaatt tcagcgcgga gcaaaccgaa aacctgctgc
    12481 tgggtatttg tggagagaaa ctcgatggcg cgggcaggaa tttcgtgcag gtgctcgagc
    12541 gcaacgaccg ccttctcttg ctgccggaaa tccgggagtt gtttgaagca ctcaaggcgg
    12601 aacaggaagg cgtgctcgaa gtgaagattg attcagcgtt tccgctgagc aagcagcagg
    12661 cgggcgcact ggtgcgccgt ctcgaaggca ggtaccagcg caaaatcgtc acgcaggtga
    12721 gcgtggaacc cgaactgatc ggcggcgtga aaatcgcagt cggcgaccat gttctcgatg
    12781 ccacggtacg cggcaaactg gaagcgatgc gtgcagcgtt gacaagataa atccgcaaac
    12841 cccaggtttt atttaggagc agaaatgcaa gttaacccct ctgaaatcag tgagctgata
    12901 aaaagcaaga tcgagaacct cgcggtgtcg gcggaatcgc gcacgcaagg cacggtgatt
    12961 tcagtgaccg acggcatctg ccgcgtacac ggcctgtccg acgcgatgca gggcgagatg
    13021 ctggaatttc ccaagaacac tttcggcttg gcgctgaacc tcgagcgcga ttcggtgggc
    13081 tcggtggtgc tgggcgaata tgagcacatc accgaaggcg ataccgtgaa gtgcaccggc
    13141 cgcatcctgg aagtcccggt gggcgaagcg ctgatcgggc gcgtggtgaa ctcgctcggc
    13201 cagccgatag acggcaaagg cccgattcag gccaaggaga cttcgcccat cgaaaaaatc
    13261 gcaccggggg taatctggcg caaatcggtg tcgcagccgg tgcaaaccgg cttgaagtcc
    13321 attgactcca tggtgccgat cggccgcggc cagcgtgagc tcatcatcgg cgaccgccag
    13381 accggcaaga ctgcggtggc gctggacacc atcatcaacc agcagggcaa ggacctcatt
    13441 tgtatttatg tcgcgatagg acaaaaagca tccagcatcg ttaacgtggt gaggaagctc
    13501 gaagagcacg gcgccatggc ctataccatc gtggtggcgg cttctgcctc cgaatccgcc
    13561 gcgatgcagt acatcgcgcc ctattccgg
//
LOCUS       DATIOW010000151         4643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1896030, whole genome shotgun sequence.
ACCESSION   DATIOW010000151 DATIOW010000000
VERSION     DATIOW010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1896030"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VLL03_05065"
     CDS             complement(<1..712)
                     /locus_tag="VLL03_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSS46766.1"
                     /translation="MKKQMDRRQFLKLTGLGGVVFVSGLAGCAGAGRKPGAAAHDDFY
                     FVQLSDTHWGFEGAAVNPDAKGTLKKAVAAVNGLDDKPDFIVFTGDLTHTTDDPKIRR
                     QRLAEFREIVSELKVNNVRFMPGEHDASLDRGEAYQEFFGKTHYTFDHKGVHFIVVDN
                     VSDPGAKIGDEQLQWLSADLQQQDKNARIVVFTHRPLFDLYPQWDWATRDGAQAVALL
                     MPFPNVTVFYGHIHQEHHF"
     gene            complement(836..1066)
                     /locus_tag="VLL03_05070"
     CDS             complement(836..1066)
                     /locus_tag="VLL03_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46767.1"
                     /translation="MLTNRNKLSALLLGIMLSFSVLQPALAAEDAKMARNSQQSGDAP
                     CAESKKQFNDNTDKMDSSPDTWYERQSRIPGG"
     gene            1229..1744
                     /locus_tag="VLL03_05075"
     CDS             1229..1744
                     /locus_tag="VLL03_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSS46768.1"
                     /translation="MDKNRRFEEIALPHLSAAYNLARWLTRNDHDAEDMVQEAYLRAF
                     KFFDSFRGEEGRAWLLAIVRNTCYTWLKQNRVLELHDSFDEEAHAEADSGSDPQALML
                     QNADKQLLDLAIETLPLEFREILIMRELEDLSYKEIAAVAEVPIGTVMSRLARARNML
                     RASLERIMQER"
     gene            1746..2513
                     /locus_tag="VLL03_05080"
     CDS             1746..2513
                     /locus_tag="VLL03_05080"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HSS46769.1"
                     /translation="MNCDETRNLMHGYLDKEIDLVRAMEVEKHLEGCAECKALYSQET
                     AVQSTIRERASYYAAPKYLEARIRAALPKPGKKAMVFRQPNLNWLSFAASMAFAVVIT
                     WGVALNFNAASEDERLTEEIISDHVRSLMANHLSDVASSDQHTVKPWFNGKLDFSPTV
                     IDLTTQGYVLVGGRLDYLNNRPVAVLVYRHRKHFINLFIWPEESREKSLVRTKTKQGY
                     NLIHWKDSGMVFWAVSDLQENELMQFASLLQQPAAPQ"
     gene            complement(2518..4239)
                     /gene="lpdA"
                     /locus_tag="VLL03_05085"
     CDS             complement(2518..4239)
                     /gene="lpdA"
                     /locus_tag="VLL03_05085"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HSS46770.1"
                     /translation="MHKLIEVKVPDIGDFKNVPVIEVLVKPGDRIAKEAPLITLESDK
                     AAMDVPSPDAGTVKEIKVKVGDKVSEGSTIVVLETAAVSQPARQPSSPPAPAPGVSAA
                     RAPGELHAEVAVLGAGPGGYTAAFRAADLGKQVILIERYPTLGGVCLNVGCIPSKALL
                     HLAKVMSEAVEMQHYGVTFGQPKIETEKLRSWKNSVIGKLTKGLAGLAKQRKVQVLQG
                     VAQFASPNTLRVETKDGAKTVSFDYCIIAAGSQAAKIPGFPDDPRLMDSTSALEIAGI
                     PKRLLVIGGGIIGLELACVYDALSSKITVVELLDGLMPGTDRDLVKPLQKRIEKRYEG
                     IYLKTKVTRIEPQKDGLKVYFEGDNAPQPQVFDCVLVAVGRRPNGQAVAAENAGVQVT
                     DRGYIAADKQMRTNVPHIFAIGDIAGEPMLAHKATHEGKVAAEVIAGHKAAFDARTIP
                     SVAYTDPEVAWMGLTEAQAKEQGVEFEKAVFPWMASGRALAQGRDEGMTKLIFDKNTR
                     RLLGAGMVGPNAGELIAETVLALEMGADAQDIGLTIHPHPTLSETVFFSAEMAEGTIT
                     DLYIPKK"
     gene            complement(4335..>4643)
                     /locus_tag="VLL03_05090"
     CDS             complement(4335..>4643)
                     /locus_tag="VLL03_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSS46771.1"
                     /translation="PILSVRVVKDVEQAIQHIATYGSQHTDAIVTEDYGRAMRFLREV
                     DSSSVMVNASTRFADGFEYGLGAEVGISTDKIHARGPVGLEGLTSQKFIVFGDGQIRQ
                     "
BASE COUNT          957 a         1297 c         1327 g         1062 t
ORIGIN      
        1 tgaagtggtg ttcctggtgg atatgcccgt agaagaccgt gacattggga aacggcatca
       61 ggagcgcgac ggcctgtgcg ccgtcgcgcg tcgcccagtc ccattgcgga taaagatcaa
      121 acagcgggcg gtgcgtgaac accacgatgc gcgcgttctt gtcctgctgc tgcagatcgg
      181 cactcagcca ttgcaactgt tcgtcgccaa ttttggcgcc tggatcggag acgttatcga
      241 ctacgatgaa atgcacgccc ttgtggtcga aggtgtaatg ggttttgccg aagaactcct
      301 gataggcttc gccgcggtca agcgaggcgt cgtgctcgcc cggcatgaag cggacgttat
      361 tcactttgag ctcgctcacg atctcgcgga attcggcaag ccgttgcctg cggattttgg
      421 gatcatcggt agtgtgggtg aggtcgccgg tgaaaacgat gaaatcgggt ttatcgtcca
      481 aaccgttaac cgccgccacc gcttttttca aggtgccttt ggcgtcggga ttgaccgccg
      541 cgccctcgaa tccccaatgg gtgtccgaaa gctgcacaaa atagaaatcg tcgtgcgcag
      601 cagcgcccgg tttccttcct gcaccggcgc agccggcaag gccggaaaca aacaccacac
      661 cgcccaggcc agtcagttta agaaactgcc gccggtccat ttgctttttc atttgagtct
      721 ccttgtgtca aagttaaggg gtctgtaacc tgcataacca gattcgcacc cgaaatattc
      781 cccgaagttg ccttcgggga attccggaaa ttaaccatcc agattcgagg tattattaac
      841 cgccggggat cctgctttgg cgctcgtacc atgtgtcagg cgagctgtcc atcttgtcgg
      901 tattgtcgtt aaattgtttc ttggattctg cgcaaggggc gtcgccgctc tgctgggaat
      961 tccgcgccat tttggcgtct tctgctgcca gtgccggctg aagcacagag aaagaaagca
     1021 tgatgcccaa taaaagagct gacaatttgt ttctattcgt aagcattttg aatctcctgt
     1081 ataaatgttg gtcgagacga cttgccaatc aagccgtcta caccctgcat aacctacgac
     1141 gcgcgacgaa tattcccgga attcgtttta atttggttta gggaataaaa tgagccaata
     1201 tccggtaata cagataacgg gaatagcctt ggataagaat cggcgctttg aagaaatcgc
     1261 gctgccgcat ctcagcgccg cttacaacct ggcgcgctgg ctgacccgca acgatcacga
     1321 cgcggaggat atggtgcagg aagcgtactt gcgtgctttc aagttttttg acagcttccg
     1381 gggcgaagag ggacgcgcct ggctgctcgc aattgtgcgt aacacctgtt acacctggtt
     1441 gaagcagaat cgtgtcctag agctgcacga ttcgtttgac gaggaggcgc acgccgaggc
     1501 ggacagcggc agcgatccgc aagcattgat gttgcaaaac gccgacaagc agttgcttga
     1561 cctcgcgatt gaaacactac cactggagtt cagggaaata ctgattatgc gagagcttga
     1621 agatctttcc tacaaggaaa tcgccgctgt tgccgaggtt ccaataggca cggtgatgtc
     1681 gcggctcgcc cgtgcgcgca acatgctccg cgcctcgctg gagcggatta tgcaggagag
     1741 gtgaaatgaa ttgcgacgaa acccggaatc tgatgcacgg ctacctcgat aaggaaatcg
     1801 atttggtgcg ggccatggaa gttgaaaagc accttgaggg ctgcgctgaa tgcaaagcgc
     1861 tttattccca ggaaaccgcc gtacagtcca cgattcgcga gcgcgcgagc tattatgctg
     1921 cgccgaaata tcttgaagcg cgcattcgtg ccgcgctgcc caagcccggc aaaaaggcga
     1981 tggttttcag acagccgaac ttaaactggc ttagctttgc cgcttcaatg gcttttgcgg
     2041 tggtaattac ctggggcgtg gcgctgaatt ttaatgcggc ttccgaagac gagcgcctta
     2101 ctgaagagat catctccgat catgtgcgct cgctgatggc gaaccacctt tctgatgtcg
     2161 cttcctcaga ccagcacacg gtgaagccct ggttcaacgg caaactcgat ttttcgccga
     2221 cggtgatcga tttgactaca cagggctatg tgctggtggg cggacggctt gattacctca
     2281 acaaccggcc ggtggcagtg ctggtgtata ggcaccgcaa gcatttcatc aatcttttca
     2341 tctggcctga agagtcgcgc gagaaaagct tggtacggac gaaaaccaag caggggtata
     2401 acctgattca ctggaaagat tcggggatgg tgttttgggc ggtttccgac ttgcaggaga
     2461 acgagctcat gcagtttgcg agcttgttgc aacagcccgc cgctccgcag tgaagcgtta
     2521 ctttttcgga atgtagagat cggtgatggt gccttccgcc atctcggcgg agaagaacac
     2581 ggtttcggaa agcgtgggat ggggatggat ggtgagcccg atgtcttgcg cgtccgcgcc
     2641 catttccaat gccagcacgg tttcggcaat caattcaccg gcgttcggcc ccaccatacc
     2701 cgcgccaaga agccgccggg tattcttgtc gaaaatgagc ttggtcatgc cttcgtcgcg
     2761 tccctgcgcc aacgcccgac cactcgccat ccaagggaaa acggctttct cgaactcgac
     2821 accctgttct tttgcttgtg cttcggtcag ccccatccat gccacttcgg gatcggtgta
     2881 ggcgaccgag ggaatggtgc gtgcgtcaaa cgccgccttg tgcccggcaa tcacttctgc
     2941 ggcgactttg ccttcatgcg tggccttatg cgccagcatc ggttcgccgg cgatatcgcc
     3001 gatggcgaag atgtgcggca cgttggtgcg catctgcttg tccgccgcga tatagcctct
     3061 gtcggtgacc tggacgccgg cattttctgc ggcgacggcc tggccgttgg ggcgcctgcc
     3121 taccgcgacc agcacgcagt cgaaaacttg cggctgcggc gcgttgtcgc cctcgaaata
     3181 aactttcaac ccgtcttttt gcggttcgat tctcgttact ttggttttaa ggtaaatgcc
     3241 ttcgtaacgc ttttcaatgc gcttttgcag aggcttcacc agatcgcggt ccgtgcccgg
     3301 catcaagcca tccagcaatt ccaccacggt gattttcgaa ctcagcgcat cgtacacgca
     3361 cgccaattcc agcccgatga tgccgccgcc gatgaccaag agtcttttgg gaatcccggc
     3421 gatttccagc gcgctggtcg aatccatgag gcgcgggtcg tcgggaaagc cgggaatttt
     3481 cgccgcctgc gagccggcgg cgatgatgca gtaatcgaaa gacacggttt tcgcgccgtc
     3541 tttggtttcc acacgcagcg tattgggcga agcaaattgg gcgacgccct gcagcacctg
     3601 caccttgcgc tgcttggcga gaccggcaag acccttggtg agtttgccga tcacgctgtt
     3661 tttccacgag cgcagttttt cggtttcgat tttgggttgc ccgaaagtca cgccgtagtg
     3721 ctgcatttcc acggcctcag acatgacttt ggcgagatgc agcagcgcct tcgagggtat
     3781 gcagccgacg ttgagacaca cgccgcccag ggtaggatag cgctcaatca aaatcacctg
     3841 cttgccgaga tcggcggcgc ggaacgccgc ggtgtagccg cccggccccg cgcccagcac
     3901 cgccacttcg gcatgcaatt cgcccggagc cctggctgcg gaaacgccgg gcgctggtgc
     3961 aggcggggag gacggttgcc gtgcgggctg tgaaaccgcg gcggtttcca agacaacaat
     4021 cgtcgaacct tcggaaactt tgtcgccgac tttgaccttg atttctttca ccgttcccgc
     4081 atccggagaa ggcacatcca ttgctgcctt gtcggattcc agcgtaatca gcggagcttc
     4141 cttggcgata cggtctccgg gtttcaccag cacctcgatc accgggacgt ttttgaaatc
     4201 gccgatatca gggactttga cttcaataag tttgtgcatg ggttatttat gagtgaacaa
     4261 gtgaagaagt gaagccccct cactccaacc ctccccccgg tgcgggggag agggtgttca
     4321 cccgttcact taattcactg ccttatttgt ccgtcgccga agacgatgaa cttctgcgaa
     4381 gtaagccctt ccagcccaac cgggccgcgt gcgtggattt tgtcggtgga aattccgact
     4441 tccgcgccca ggccatattc gaagccgtcg gcgaagcgcg tcgaggcgtt caccatcacc
     4501 gagctggaat ccacttcgcg caggaaacgc atggcgcggc cgtaatcttc cgtcacgatg
     4561 gcgtcggtgt gctgcgagcc gtaggtggcg atgtgctgaa tagcctgctc cacgtctttg
     4621 accacgcgca cgctcaaaat cgg
//
LOCUS       DATIOW010000152         2776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1694431, whole genome shotgun sequence.
ACCESSION   DATIOW010000152 DATIOW010000000
VERSION     DATIOW010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694431"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VLL03_05095"
     CDS             <1..907
                     /locus_tag="VLL03_05095"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HSS46772.1"
                     /translation="LHALLRDLGDISGVEVITTRDPRLPALDFPHSVYTPRSAAETEE
                     VLEHCMDLADAVWPIAPESGGVLERIHRNVIKRGRRLLGSHPDAVRLATSKRETSSHL
                     AQAGITAVPTYSADAALHDTSGSWVVKPDDGAGCSDTHLFHNLLAARQWLQANPGNYV
                     LQPFIPGSALSLCILCREGTADLLSCNNQRVVVRDGQFHFLGSVVNALADGDGAFAEL
                     ARRVAAAIPGLWGYVGIDLLQSGSQRVVMEVNPRLTTSYAGLREALGCNPAALVLGLL
                     DDAKPLRLPKFTPQRIEVDLESLHA"
     gene            900..1946
                     /locus_tag="VLL03_05100"
     CDS             900..1946
                     /locus_tag="VLL03_05100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HSS46773.1"
                     /translation="MPDYTVGWDLGGAHLKVALVDGAGRVCKAMQLPCPLWQGLQHLD
                     SAVAQALGELNQPAALHVLTMTGELADIFPNRSAGVTALVEAMNGKLPHASLQVYAGC
                     SGFVTPAQAKQHVGEVASANWLATAELAAQHVRQALLMDIGSTTTDLVLLISGKVHAQ
                     GWNDHQRMACDELIYSGVVRTPVMAVAVRVPFAGEWQGLMAEQFATMADVYRLTGDLP
                     EGADQLPAADGGGKTMQDSARRLARMLGRDLESAPLSSWQQLANYIADLQQQRFHETC
                     SRLFSLGLAGEEAPLIGTGIGRFLVKTLAVRYARPYLDFSGLVESSLSDRQWIDACAP
                     AVAVALLALRKKTD"
     gene            2109..>2776
                     /locus_tag="VLL03_05105"
     CDS             2109..>2776
                     /locus_tag="VLL03_05105"
                     /inference="COORDINATES: protein motif:HMM:NF016593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HSS46774.1"
                     /translation="MGGVSRQRQVFGSQSRYDILVAEPYATLVTRGKVTEIRNHGASE
                     LSPEDPFTLLKRHLEPAQENHYGLPFSGGALGYFGYDLARRLERLPVIAEDAEKLPEM
                     AIGIYDWAVVVDHHSRRSYLASCGRDAETQRKWNELINCFSQPAEERFRAPFRITSKI
                     ASNLTPEHYAQAFRRIHDYIRAGDCYQVNLAQRFSATAAGDPWLAYQALRIINPAPFS
                     AYLNT"
BASE COUNT          543 a          779 c          878 g          576 t
ORIGIN      
        1 gctgcacgcg ttattgcgcg acctcgggga catttccggg gtggaagtaa tcactacgcg
       61 cgatccgcga ctgcctgcac tggattttcc gcacagcgta tacacgccgc gcagcgcagc
      121 ggaaaccgaa gaggttctgg aacactgcat ggatttggcc gatgcggtgt ggccgatagc
      181 gccggaaagc ggcggggtgc tggaacgcat tcatcgcaac gtgattaagc gcgggcgcag
      241 actcttgggc agccatcctg acgcggtgcg cctcgccacg agcaagcggg agacctcatc
      301 ccacctcgcg caggcgggaa tcacggcggt gccgacgtat tcagcggacg ccgcattgca
      361 tgatacaagc gggagctggg tggtgaaacc cgatgatggc gcaggctgca gcgataccca
      421 cctgttccat aatctcttgg cggcacggca gtggctgcag gccaacccgg ggaattatgt
      481 gttgcagccg tttattcctg gaagcgcgct gagtctttgc atcctgtgcc gcgaaggcac
      541 tgctgatctt ttgagctgca ataatcagcg cgtggtggtg cgtgacggcc agtttcattt
      601 tctcggcagc gtggtcaatg ccttggctga tggagacggc gcttttgcag aattggcaag
      661 acgcgtggcc gcagccattc ccgggctgtg gggctatgtg ggtatagatc tgttgcaaag
      721 cggttcgcag cgggtagtga tggaagtaaa cccgcggctg actacgtcct atgcgggttt
      781 gcgcgaagcg ctgggctgca atcccgccgc attggtattg ggcctgctgg atgacgccaa
      841 acctttgcgc ctgccaaaat tcacgccgca gcgaatcgaa gttgacctcg aatccctgca
      901 tgcctgatta caccgtcggc tgggatttgg gaggcgcgca tctcaaagtg gcgttagtgg
      961 acggcgccgg gcgcgtatgc aaagccatgc aattgccctg cccgctgtgg caaggattgc
     1021 agcatctgga tagtgctgta gcccaggcac tgggagagct aaaccaaccc gcagcgttgc
     1081 acgtgctcac catgaccggc gagctcgcag atatttttcc caatcgcagc gccggcgtga
     1141 ccgcgttggt ggaagcgatg aacggcaagt tgccgcatgc aagcttgcaa gtgtatgccg
     1201 gatgcagcgg gtttgttacc cccgctcaag ccaagcagca tgtcggtgag gtggcttcag
     1261 cgaattggct tgcaaccgcc gagttggcgg cacagcacgt gaggcaagcg ttattgatgg
     1321 acatcggcag caccaccacc gatctggtgt tgctgatttc aggcaaagta cacgcacagg
     1381 gctggaatga ccaccagcgt atggcgtgcg acgagctcat ctacagcgga gtagtgcgca
     1441 cgccggttat ggcggttgcc gttcgcgtgc cgttcgctgg cgagtggcag gggttgatgg
     1501 cggagcaatt cgcgaccatg gcggatgtct atcgcctgac cggagatttg ccggaagggg
     1561 cagatcagtt gcctgccgcg gatggcggag gcaagaccat gcaggacagt gcgcggcgtc
     1621 tggctcgcat gctgggcagg gatctggagt cggcgccgct ctcaagctgg cagcagcttg
     1681 cgaactatat tgccgatctg caacagcagc gtttccacga aacctgctcg cgtctttttt
     1741 cactggggtt agcgggcgag gaggcgccgc ttatcggaac aggcatcggc cgctttctgg
     1801 tgaaaacgct tgccgtacgc tatgcacggc cttatctgga tttttcaggc ctggtggaat
     1861 cgtcgttgag cgatcgccaa tggatagacg cctgcgcgcc tgcggtggcg gtcgcgctgc
     1921 tggcgctgcg gaaaaaaact gattaaccgc agaggcgcta gatacgatgt ttttcttgaa
     1981 gaattgactt gctccgcacc tcggcgtctc tgcggtgata gaatttgatt catgataacc
     2041 cctttgctca cagaacttcc ttatcacgaa gacagcgcgc ggctgtttga agcgattgcc
     2101 gacctgcctt gggcggcgtt tctcgacagc ggcaggtttt cggctcgcaa tcgcgctacg
     2161 atattctggt ggcggagccc tatgccacgc tggtcacgcg cggcaaagtg accgaaatac
     2221 gcaatcacgg cgcaagcgag ctatctcccg aagacccgtt caccctgctt aagcgccatc
     2281 tcgagcccgc gcaggaaaac cattacggtt tgcctttcag cggcggagcg ctgggttatt
     2341 tcggctatga tctggcgcgc cggctggagc ggctgccggt gattgccgaa gacgccgaga
     2401 aactcccgga aatggccatc ggcatttacg actgggcggt ggtggtggac catcactcgc
     2461 gccgtagtta tctcgcaagc tgcggccgcg atgcggaaac gcaaagaaaa tggaatgagc
     2521 tgattaactg tttcagccag ccggcagaag aacgctttcg cgcgccgttc cggataacga
     2581 gcaagatcgc ttctaacctc acgcccgagc attatgcgca ggctttccgg cgcattcacg
     2641 actacatccg cgccggcgat tgctaccagg tgaatctcgc gcaaaggttt tccgccacgg
     2701 cggcgggcga tccgtggctg gcttaccagg cgctgcgcat catcaatccg gcgccgtttt
     2761 ccgcctatct caacac
//
LOCUS       DATIOW010000153         2867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1796113, whole genome shotgun sequence.
ACCESSION   DATIOW010000153 DATIOW010000000
VERSION     DATIOW010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1796113"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /gene="hemC"
                     /locus_tag="VLL03_05110"
     CDS             <1..545
                     /gene="hemC"
                     /locus_tag="VLL03_05110"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HSS46775.1"
                     /translation="RESQLRARFPHLKVNSLRGNVGTRLNKLDQGHYTAIILAAAGLK
                     RLGLEGRITALLSPEQSLPAVGQGALGIECLSKREDLLQLLQPLNHRETALCITAERA
                     MSRALGGSCQVPLGGYAQMQGEEIHLRGFVATSDGGRLVSAEARGNAGKAEALGEALA
                     KALRAKGADKILAQLESRGA"
     gene            542..1354
                     /locus_tag="VLL03_05115"
     CDS             542..1354
                     /locus_tag="VLL03_05115"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HSS46776.1"
                     /translation="MMDTRPKVVAAERKRALSGCNIVVTRPQDQAKELADLIRAEGGC
                     AILFPALEIIEAEDLRPLHQLIGRLEQFDLAIFISANAANKGMSEIMARRALPDTLQI
                     AAIGAGSQKALQRFGAKQVIYPVSRSDSEALLELPQLRDVAEKRIVIFRGQGGRELLG
                     EMLAKRGARVEYVECYRRAKPDADPAPLKRLWADDKLHAITVTSSEGLRNLFEMMDET
                     GRELLKKTPLFVPHPRIAEAARELGLANPVVTAAGDAGLVKGLVEWFGTKAR"
     gene            1361..2395
                     /locus_tag="VLL03_05120"
     CDS             1361..2395
                     /locus_tag="VLL03_05120"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HSS46777.1"
                     /translation="MTDNSQLETPSSLVKQEKRFILALLILLAVAALLAWQWFDARDQ
                     IAALQQEFAKRLADTASIVNDNRVIVKYNQEQLRETQVKLGLLENKLAESQNLQIALE
                     SLYQELSRNSDEWALVEVQQMLDIASQQLQLAGNVKAALIALQTADARLQRIDRPQLI
                     PLRKIINKDIERLKAVPYVDTVAISVQLDNLISKVDSLPLAMDVRRHSAQSEAGERSA
                     QSAWLKFTREIWQDMKQMVRIQNTAEQEIPLLAPDQLFFLRENLKLRLLSARLALLLH
                     DETSFKSDINSVQKWLSRYYDNDSEATKAALASLRQLQARRISIGVPDISASLDAARN
                     FKLAREKAAK"
     gene            2392..>2867
                     /locus_tag="VLL03_05125"
     CDS             2392..>2867
                     /locus_tag="VLL03_05125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HSS46778.1"
                     /translation="MRFFIWIVAIFALAVALAVTLGYDNGYVLLVYSTYRVELSVKSA
                     VLLLLGGFVIAYALIRLLVHTLRLPAQVREYRVRRRSDKARSTFLEGLRMFFEGRYGK
                     AEKAAAAALEMGESPGITALLAARSAHELKAFDKRDAYLDEAERRAPEEKAARLMT"
BASE COUNT          628 a          776 c          915 g          548 t
ORIGIN      
        1 gcagggaaag ccagctgcgc gcgcgctttc cgcatctcaa ggtgaattcg ctgcgtggca
       61 atgttggaac ccggctgaac aagctcgatc aaggtcatta caccgcaatc atactggcgg
      121 ccgccggctt aaaaaggctg ggactggaag ggcgcatcac cgcgctgttg agccccgagc
      181 aaagcctgcc cgcggtgggt caaggtgcgc tgggaatcga atgcttaagc aagcgtgaag
      241 atttgctgca gcttttgcag ccgttgaacc accgtgaaac cgcgctttgc attaccgcag
      301 agcgcgccat gagccgggcg cttggcggca gctgccaggt gccgcttggc ggctatgcgc
      361 agatgcaagg cgaagagata catttgcgcg gtttcgttgc gacgtccgac ggcggccgct
      421 tggtgagcgc cgaagcgcgc ggcaacgcgg gaaaagcgga agcattgggt gaagcattgg
      481 caaaagctct gcgagccaaa ggcgcagaca agatactcgc ccagctggag tcgcgaggcg
      541 catgatggat accaggccca aggttgttgc tgcggagcga aagcgcgcgt tgagcggctg
      601 caatattgtg gtgacgcgcc cgcaggacca ggccaaggag cttgccgatt taatccgcgc
      661 cgagggaggc tgcgctatcc tgtttccggc gctggaaatc atcgaggccg aagacctgcg
      721 accgctgcat caattgattg gccgtctcga gcaattcgac ttggcgattt tcataagcgc
      781 taatgcggcg aacaaaggaa tgagcgagat tatggcgcgc cgcgcgttgc ccgatacgct
      841 ccaaatcgcc gcgatcggcg cgggcagcca aaaagcattg cagcgtttcg gcgcgaagca
      901 agtgatttat cccgtttcgc gatccgacag cgaagcgctg ctcgaacttc cgcagttgcg
      961 agatgtagcg gagaagcgca tcgtgatttt ccgcggccag ggcgggcgcg aattgctagg
     1021 cgagatgctg gcaaaacgcg gcgcgcgcgt ggaatacgtg gaatgttacc ggcgcgcaaa
     1081 acccgatgcc gacccggcgc cgcttaaacg gctgtgggcg gatgataaat tgcacgccat
     1141 tacggttacc agcagcgaag ggctgcgcaa tttattcgag atgatggacg aaacggggcg
     1201 ggagctgctc aaaaaaaccc cgctgtttgt gccgcacccg cgcatcgccg aggcggcacg
     1261 ggagctgggg ctcgccaatc cagtggtgac cgcagcaggg gacgcaggcc tggtcaaagg
     1321 attggttgaa tggtttggga caaaagcacg ataggcagcc atgaccgata attcgcaact
     1381 ggagacgcca tcttcgctcg tcaaacagga gaaacggttc atcttggcgc ttttaatctt
     1441 gctggccgtg gcggctcttt tggcctggca gtggtttgat gcccgcgacc agatcgcagc
     1501 attacagcag gagttcgcca agaggttggc tgatactgct agcatcgtca atgacaaccg
     1561 cgtcatcgtc aagtacaacc aggaacagtt gcgcgaaacg caggtgaagc tgggtttact
     1621 ggagaacaag ctcgcggaat cgcaaaacct gcagattgca ttggaaagct tgtatcagga
     1681 actttcccgc aacagcgacg aatgggcgct cgtggaagtg cagcagatgc tcgatatcgc
     1741 gagccagcaa ttgcaactgg cgggcaacgt caaggccgcg ctgattgctc tgcagacggc
     1801 ggatgcgcgc ctgcaacgca ttgaccgccc gcagctcatt ccgctgcgca agattatcaa
     1861 caaggatatc gagcgtctca aggcagtgcc ttacgtggat acggtggcga tcagcgtgca
     1921 gctggataac ctgatatcca aggtggacag cttgccgctg gcaatggatg tgcgccggca
     1981 ttcggcccaa tccgaagcag gtgagcggtc tgcgcagagc gcatggctta aattcacgcg
     2041 cgaaatctgg caggacatga aacaaatggt gcgcatacaa aataccgccg agcaggaaat
     2101 accattgctt gcgccggacc agttgttttt cctgcgcgaa aatctcaagc tgcgcctgtt
     2161 aagcgcgcgg ctcgccctgc tgttgcatga tgagaccagc ttcaagtccg atattaattc
     2221 cgtgcagaag tggctctcgc gctattacga caacgactcc gaggcgacca aggccgcgct
     2281 ggccagcctg cgccagttgc aggcacgccg gatcagcatc ggggttcccg acatctcggc
     2341 gagcctggat gcggcgcgta acttcaagtt agctcgcgag aaggcggcaa agtgaggttc
     2401 ttcatctgga ttgtggcgat ttttgccctg gcggtagctt tggcagtaac gctcgggtat
     2461 gacaacggct acgtgctgtt ggtctattcc acctaccgcg tcgaactctc ggtcaagtcg
     2521 gcggtgctgc tgttgttggg cgggtttgta atcgcttatg cattgattcg cctgctggta
     2581 cacacgctgc gcctgccggc gcaagtgcgc gagtaccgcg tgcgccgtcg cagcgacaaa
     2641 gcgcgcagca ccttccttga agggctgcgg atgttttttg aagggcgtta cggcaaggcg
     2701 gagaaagccg ctgccgccgc tctggaaatg ggggaatccc cggggatcac tgcgcttctg
     2761 gccgcacgct cggcgcatga gttaaaagct tttgacaagc gtgatgccta cctagacgaa
     2821 gcggaaaggc gggcgccgga ggaaaaagcg gcgcgtctga tgacgca
//
LOCUS       DATIOW010000154         2046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1694657, whole genome shotgun sequence.
ACCESSION   DATIOW010000154 DATIOW010000000
VERSION     DATIOW010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694657"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VLL03_05130"
     CDS             complement(<1..341)
                     /locus_tag="VLL03_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46779.1"
                     /translation="MNFDWTAITEEVRSTLSHQEAPYLLVLMVVMTVLLLRFRPAERR
                     NVLGTLRFFLLSLLGMLVAGVLHVIFSGIITSVLHEAFLIATGVAAIKLTTLFAFRIA
                     LPLVRLHTARI"
     gene            complement(576..1922)
                     /locus_tag="VLL03_05135"
     CDS             complement(576..1922)
                     /locus_tag="VLL03_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HSS46780.1"
                     /translation="MNRYPILLFISMMSSFSARAADLLEIYRQALVQDAVYASARATW
                     QAGQEKLPQGRALLLPALNLSGNTIYNDVNTQIRGTSVLQGGPRAYNTNGITVSLTQP
                     LFRKQNIDQYLEAKSQLAQNDAQLDVAAQDLILRVSQAYFDVLAAQDNVAFAAAQKAA
                     IAEQLASAKRNFEVGTATITDTNDAQARYDLVVSQEIAAQNDLEVKKQVLAQIIGSAP
                     AVLKLLATDLPLSLPKPNDMERWVEAAQANSPQIRAQEAALEFTKKEVDRNRGGHYPT
                     VDLVGTYSNTAASGSATFGSGNGTGNDTTLKTIGIQLNLPLYQGGGQQSRVREAIANE
                     DKARQDLENTRRSVIYSTRQAFLGVTSGVAQVKALEQALVSNQSSLDSTKLGQEVGVR
                     TGVDVLNAQQQLFSAKRDLAQARYNFILSQLRLKSAAGQLSEDDLTEVNRWLRQPQ"
BASE COUNT          426 a          601 c          571 g          448 t
ORIGIN      
        1 atgatgcgcg ccgtatgcag ccgcaccagc ggcagcgcga tgcggaacgc aaacaaggtg
       61 gtcagcttga tcgccgcgac cccggtggcg atgagaaagg cttcgtgcaa aaccgaagtg
      121 atgatgccgg agaaaatgac atgcaggacg cccgcgacca gcatgcccaa gaggctcaac
      181 aggaaaaaac gcaaggtgcc gagcacattg cgccgctccg ccgggcggaa gcgcagcagc
      241 aaaacggtca ttaccaccat tagcaccagg agatatggcg cctcctgatg cgacagcgtg
      301 gagcgcactt cttcggtaat cgcggtccag tcgaaattca cggcagctcg tctgaattca
      361 aggccagtgc ttaatttacc atgattgcag cggcgggagc atggcgcaca gattgcccta
      421 ggcgggattt ttcaccctga accaagcagc gaaaggtgcg gcgactttca catattcgcc
      481 cacggcgtaa ccctgataat ccgggttatt ttacagcgaa caggcgccaa gcagaagcac
      541 cagcacgctc caaaataagc cccgtcggtc tggatttatt gcggctgcct taaccaccga
      601 ttgacctcgg tgagatcgtc ttcactgagc tgccccgccg cggacttgag ccgcaactgg
      661 ctgagaataa agttgtagcg cgcctgagcc aggtcgcgct tggcgctgaa cagttgctgt
      721 tgggcgttca gcacatcgac tccggtgcgc acgcccactt cctggccgag cttggtggaa
      781 tccagcgaac tttggttcga caccagcgcc tgttccagcg ccttcacctg cgccacacca
      841 ctggtcaccc ccaaaaatgc ttgacgagtg gaataaatca cgctgcgacg ggtgttttcc
      901 aagtcctgcc gcgccttatc ttcattggca atcgcttcac gcacccgcga ttgctggccg
      961 ccgccttgat acaaaggcag attaagctga atgcctatgg tcttgagtgt ggtgtcgttg
     1021 ccggtgccgt tgccactgcc gaaggttgca ctgccggacg cggcggtatt gctgtaagtg
     1081 ccaaccaaat caaccgtggg gtaatgtccg ccgcggttgc ggtcaacttc ctttttggtg
     1141 aattccagcg ccgcttcctg cgccctgatt tgcgggctgt tggcttgcgc cgcctccacc
     1201 caacgctcca tgtcattggg ctttggcagg ctcagaggca aatcggtggc gagcaacttg
     1261 agcaccgcag gcgcactgcc gatgatttgc gccagcacct gcttcttcac ttccaaatcg
     1321 ttttgcgcgg cgatttcctg tgatacgacc agatcgtagc gagcctgtgc atcattggtg
     1381 tcggtgatgg ttgctgtgcc aacttcgaaa ttgcgtttgg cggaagccaa ctgctcggca
     1441 atcgccgctt tttgcgcggc agcgaatgcc acattgtcct gcgccgccag cacgtcaaaa
     1501 tacgcctgcg acacccgcaa aatcagatcc tgcgcggcca catccaactg cgcgtcgttt
     1561 tgcgccagct gcgatttcgc ttccaggtac tggtcgatgt tttgcttgcg aaacagcggc
     1621 tgggtcagcg aaacggtgat tccgttggtg ttgtaggccc gcggaccacc ctgcagcacc
     1681 gaagttccgc gtatctgcgt gttgacatcg ttatagattg tattgccgga aaggttcagc
     1741 gccggcaaca gcagcgcgcg tccttgcggc aatttctctt gcccggcctg ccaagttgcg
     1801 cgcgccgaag cgtaaaccgc atcctggacc agcgcctgcc ggtaaatttc gagtaaatcg
     1861 gcggcgcgcg ccgaaaagct tgacatcatg cttatgaata acaatatcgg gtagcgattc
     1921 atggtagcgt cctaattgtt aaatgttcat cttgacatcc gttttttccc gcggctgcgg
     1981 attccctcca gcaaaatatc caaatgcatg tccaggtagt gcgtaacgtt gagcgccttg
     2041 cgttca
//
LOCUS       DATIOW010000155        17507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1390582, whole genome shotgun sequence.
ACCESSION   DATIOW010000155 DATIOW010000000
VERSION     DATIOW010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17507
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390582"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VLL03_05140"
     CDS             complement(<1..936)
                     /locus_tag="VLL03_05140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HSS46781.1"
                     /translation="MNELILKPRREKSLLRRHPWIFSGAVEKIQDSPQAGDTVEIVSS
                     NGEFLAHAAFSPHSQIVARVWDWQKQGRIDAGFFRSRLAQAVETRKKLIQDTDALRLV
                     HGESDGLPGLIVDRYGDTLVLQALSAGAERWRETCADILIELTGAARVFERSDAEVRQ
                     LEGLPPRMGVLRGSPPSEVIKIREGRLQFQVNVQAGQKTGFYLDQRGNRTRIGELADG
                     KEVLDCFCYSGGFSIHALAGGAKSVISVDASADALMLARQNLELNQLTRESVELREGD
                     VFEVLRALRDRGKRFDLIILEPPKFAPTAKFSERAA"
     gene            complement(1091..2008)
                     /gene="xerC"
                     /locus_tag="VLL03_05145"
     CDS             complement(1091..2008)
                     /gene="xerC"
                     /locus_tag="VLL03_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HSS46782.1"
                     /translation="MRSKSTEGSIARSSRNAHLARYLSYLATERRLSRHTCENYARDV
                     VMLLQLAGTTLLKDLQIQHIRRFVAQLHAQGLSGKTLARMLSAWRGFYRYLARDSGFT
                     HNPCQGLRAPKSAKTLPHALSPDEANRLMEISTDGPQALRDKAILELLYSSGLRLSEL
                     AGLLVPDINLDNATVRVTGKGSKTRIVPVGSHALNAIRAWLSERDKTARSDALFVNRD
                     GKPISTRAIEYLVKKWAVKQGISSHVHPHMLRHSFASHLLQSSGDLRAVQEMLGHASI
                     STTQIYTHLDFQYLAKAYDKAHPRAKKKP"
     gene            complement(2019..2669)
                     /locus_tag="VLL03_05150"
     CDS             complement(2019..2669)
                     /locus_tag="VLL03_05150"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HSS46783.1"
                     /translation="MKIKAEDVAAYLEAHPEFFEDYADLLANIYIPHPHGGRAIPISE
                     RQILTLRERNRQIENKLGELIQFGEENDAIGERVHRMALALLGARNLEGLLHSLYFNL
                     REDFAVPHVMLRLWGVNGNPQALPEFGEVKEELRASAEALINPHCGAQFGGTDWFGED
                     ATRLRSFALVALRSERTFGLLVLASEDAQRFYPEMGTLYLKRLGELASAALLRHLT"
     gene            complement(2682..3512)
                     /gene="dapF"
                     /locus_tag="VLL03_05155"
     CDS             complement(2682..3512)
                     /gene="dapF"
                     /locus_tag="VLL03_05155"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HSS46784.1"
                     /translation="MLLKFTKMHGFGNDFVVLNGISQRIILTSNQYRAIADRHFGIGC
                     DQILVVEKTARSDADFRYRIFNADGSEVEQCGNGARCFVRFVRDKGLTNKTEIRVETK
                     GGIIVPRLEADGQVTVNMGVPRFAPEKIPFEAKKRALTYSIIVKGRQIKISAVSMGNP
                     HAVQVVKDVDGAPVTAQGPLIEKHARFPQGVNAGYMQVVDRGRIKLRVYERGVGETLA
                     CGTGACAAVAAGIQLDLLDKRVVVQTQGGELTICWEGEGKPVWMTGDATTVYEGIIKI
                     "
     gene            complement(3655..4524)
                     /locus_tag="VLL03_05160"
     CDS             complement(3655..4524)
                     /locus_tag="VLL03_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HSS46785.1"
                     /translation="MMVRIGLVLVWLLHFLPLGVLAKLGEWMGMLLFVFGHERRHVAR
                     TNLQLCFPQWSDEKRENILRQHFRAFGRNFLEHGILWWSSRERIERLVHIEGLENWQA
                     VRNQPVIWLAPHFVGLDMGGVRITSEYPLVSMYSQQKNSLVNGLLLHGRTRFGTAILV
                     SRQEGIRPIINALREGVPFYYLPDMDFGPRDSLFVPFFGVSAATIPGLSRLARMTGAK
                     IVPCVTRQLPGGAGYVLKFYPAWDDFPGDNTEADTRRMNVFIEERVLEMPEQYYWLHR
                     RFKTRPPGEPKPY"
     gene            4658..5845
                     /gene="metK"
                     /locus_tag="VLL03_05165"
     CDS             4658..5845
                     /gene="metK"
                     /locus_tag="VLL03_05165"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006024140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HSS46786.1"
                     /translation="MMNEYLFTSESVSEGHPDKVADQISDAILDAILAQDKTCRVAAE
                     TLCNTGLVVLAGEITTNANVDYIGVARETIRRIGYDNTEYGIDYKGCAVMVCYDKQSP
                     DIKQGVDKAQDDPLNQGAGDQGLMFGYACDETPQLMPLPIFLAHRVVERQSEIRRDGR
                     LPWLRPDAKSQVTIRYVDGKPKEIDTIVLSTQHAPDIGHDKLKEAVIEEIIKPVLPKN
                     MVGADIKYLVNPTGKFVVGGPHGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDR
                     SATYAARYVAKNIVAARLASKCLVQVAYAIGVARPVSLMVNTFGTGKISDDKIVKLIE
                     VHFDLRPKGIIQSLNLLRPIYLKTASYGHFGRDEPEFTWENTDKAMALADTAGMKRET
                     MIA"
     regulatory      5859..5942
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            5961..7376
                     /gene="ahcY"
                     /locus_tag="VLL03_05170"
     CDS             5961..7376
                     /gene="ahcY"
                     /locus_tag="VLL03_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HSS46787.1"
                     /translation="MMSAVLKSQQDYKVADISLADWGRKEIAIAETEMPGLMSVREEY
                     RAQQPLQGARIAGSLHMTIQTAVLIETLKALGAEVRWASCNIYSTQDHAAAAIAAGGT
                     PVFAYKGETLDEYWEYTHRIFEWADGGFPNMILDDGGDATLLVILGSKAEKDPTVIAK
                     SANEEEKALYASIKKRLDTKPGWYSKALANIKGVTEETTTGVKRLYQMQKEGRLPFPA
                     INVNDSVTKSKFDNLYGCRESLVDGIKRATDVMIAGKIAVVCGHGDVGKGCAQSLRGL
                     GATVWVTEIDPICALQAAMEGYRVVTMEDAADKADIFVTATGNFHVIALEHMKKMKHN
                     TIVCNIGHFDSEIDIASLRQYRWENIKPQVDHVIFPDGKRLIVLAEGRLVNLGCATGH
                     PSFVMSNSFTNQVIAQIELYQHGAKYEKKVYVLPKHLDEKVAWLHLKKIGAKLTELTD
                     EQAKYIGVNKAGPFKPEHYRY"
     gene            7432..8277
                     /gene="metF"
                     /locus_tag="VLL03_05175"
     CDS             7432..8277
                     /gene="metF"
                     /locus_tag="VLL03_05175"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HSS46788.1"
                     /translation="MQSQKKYPRTFSFEFFPPQTPEGVEKLRATRIQLAQLKPEFFSV
                     TFGAGGSTRDRTLETVLEIRREGHNAAPHISCISSTRQNVRAILDQYRESGITRLVAL
                     RGDLPSGMAAPGQFRYANELVEFIRKETGRRFHIEVACYPEFHPQAKSARDDLLNFKR
                     KVEAGADSAITQYFFNADSYYSFVDECEALGIKIPIVPGVMPINKFTQLARFSDNCGA
                     EIPRWVRKKLEGFGDDTASIRALGLDVVTKLCDDLLESGAPGLHFYTLNQAGLTSTIW
                     QRLGL"
     gene            8282..10114
                     /locus_tag="VLL03_05180"
     CDS             8282..10114
                     /locus_tag="VLL03_05180"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSS46789.1"
                     /translation="MRRILGDPLNLLALAIVLAVVVEFSWLNWLRTPEARFSDLFVAA
                     QAERLRPDSDIVIVAAAEDSLERMADYAGRWPWPRSVHGELVQGIAAQKPRAIVFDIL
                     FFEPDIYRLDADELFNKAVSPHKNVFFPTARQDAAADPYGVPLAEIQGALGAVAGARA
                     DRKATLNIGLPKALLPENWRLGTIDFLADRDGVGRRYFVYQDAYGWKIPSLPARVAQD
                     LGLAVPDVKSILLSWPGGKAARAYVSYADLYVDFNSQKRKRDPNEFKDKIVIIGVTAT
                     GLHDIRPTPVNIRSDGVDILATAIDNLKNRNYLSQISVAWPAALTLIMLALLYAGFRA
                     RFNTLKIGAALAGASAVLLIGQYIAVSKLLLVPVLRPLLFGWAFYFITALQEYLRERR
                     GREQAIAQFSRFVNPHVVKELIAHGGLSREGQNRQVTLLFSDIRSFTALSESRTPQQV
                     VDLLNRYFSRQVDVIFRHGGALDKFIGDAIMAIWGAPLDDPKHAENAIRCALDMADTL
                     EAFRKELGDMGGEFDVGIGIHSGSAVVGLIGSDQRREYTAIGDTVNLASRIEGLTKGV
                     ARILVSEDTLRLCPDAFDFVPRGRYKVKGRTQEVELFEPKRKSA"
     gene            10111..10635
                     /locus_tag="VLL03_05185"
     CDS             10111..10635
                     /locus_tag="VLL03_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HSS46790.1"
                     /translation="MKRWAWLATVLTLLIPPAHAAETGYTLGPTELKAKPFLDAETVT
                     TLPEKSQVEILTRQGAWMQIKTKEKQQGWVRMLSVRLGNPDQKPQGGNLLSAIGFSNR
                     PRPVTTATVTTGVRGFSEEDLAKAVPNPAEVVKMKGFAATPANAQQLAAKGKLGQRQV
                     AYFDENGKPLKEIK"
     gene            10632..11492
                     /locus_tag="VLL03_05190"
     CDS             10632..11492
                     /locus_tag="VLL03_05190"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HSS46791.1"
                     /translation="MSSLRFAFILLLSALASGGCSQQQTQAFFRNINESGGLQQSLKS
                     LREPTQAEEIEIGDGIAETLLGARPLFNDAELQRYVNAVGMWVAQQSERPDLPWHFGV
                     NDSDHINAFAAPGGYIIVTKGMMKQLRNEAELAGVLGHEVAHVTQKHHLKALRKSAVM
                     NLLSAGVAAATAESRHADLVQKLAGPTKELYARGLDKGDEYEADRMGVVLAARAGYDP
                     YGLPAVLTTLASADPRDNFLTLLYKTHPLPQDRLDKLAPGLATLDGINAPQNMMRFQQ
                     YTKKLHLSSQ"
     gene            11633..12310
                     /locus_tag="VLL03_05195"
     CDS             11633..12310
                     /locus_tag="VLL03_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46792.1"
                     /translation="MSRNSKKAKSGAVRRALRPDLDLSQREFDTLKRLRTPHKIQEYL
                     NGIKANFEPHGDTVLSVREVLKQRRAHCIEGALLAACALWIQGEPPLLLDLKAVHDYD
                     HVVALFRRGGCWGAISKTNHPQLRHRDAVYRSLRELAISYFHEYTNKKGRQTLRQYSR
                     AFDLRKVDPKLWVTNGDDCWEVADILDEIHHYPLITAKQTRLLHRRDAIERKAGKLLQ
                     YPAPRRK"
     gene            complement(12446..12901)
                     /locus_tag="VLL03_05200"
     CDS             complement(12446..12901)
                     /locus_tag="VLL03_05200"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HSS46793.1"
                     /translation="MKRHSYSLLLLTLFVIVPSAWAGPKDEVAAATAKWAELFTDDNP
                     DTILALYAKDAVLWGTLSPKRRDNPEAVRDYFVNAYVALPSHKVSFGDQLIRVYGNIA
                     INTGYYTFTYVKDGEAKSLPSRYSFTYVKKGKKWLIIDHHSSVMPTPLK"
     gene            complement(13103..13846)
                     /locus_tag="VLL03_05205"
     CDS             complement(13103..13846)
                     /locus_tag="VLL03_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS46794.1"
                     /translation="MKTSPIKPPRTLLSLAGKAIADYAMIRPGDRVLLGLSGGKDSLS
                     LLHVLLHLKAKSPVKFELAAATVDPQSPEYHPEPLKAYMAGLGVPYFYESYPVLELAK
                     EKMGNASFCAFCSRLRRGMLYGAARREGCNVLALAQHLDDFAETFMMSAMFGGRLRTM
                     RAHYVNDDGDLRVVRPFVYVRERQTNAFAKSTGLPVIAENCPACFAMPMQRWNIKQKL
                     AQWEGENRQLFPSLLTAMRPLLTVLPGDD"
     gene            complement(14008..14748)
                     /locus_tag="VLL03_05210"
     CDS             complement(14008..14748)
                     /locus_tag="VLL03_05210"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF006598.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HSS46795.1"
                     /translation="MQGKVVLITGGARRVGAAICRHLHARGAGIMVHYRTSSKEARSL
                     ASELNLQRADSVALIQADLLHLSGLPNLVNDTVKRFGRLDVLINNASSFFPTPVGEIS
                     DKVWEDLVGTNLKAPLFLSQAAVAQLKKNRGCIVNIVDIHAERPMKNYVVYSTAKAGL
                     VNLTRSLARELAPEVRVNGVAPGAIVWPEDEAWSDEVSRQRIIHSTLLKRVGDPEDIA
                     KAVYFLIDDAPYITGQIIAVDGGRSINI"
     gene            14830..15987
                     /locus_tag="VLL03_05215"
     CDS             14830..15987
                     /locus_tag="VLL03_05215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HSS46796.1"
                     /translation="MPLPPPSAEAQQHSDQLCRLIRGEIAANGGRISFARYMELALYA
                     PGLGYYNAGARKLGAGGDFLTAPELSPLFGRTLAQQAKQILERGYDQILEIGAGSGAL
                     AAALLEELERLGRLPRHYHILEISPDLRSRSRDRLAAHVPHLLERVAWLNQLPPAFSG
                     LIIGNEVLDAMPVNVVLMRKRDILEADVQYDTDKNGFDWHETRAPDPLREAASRLNLP
                     ADNYRTEIGLAAQGFLRSLASTLQEGVALFIDYGFPAREYYHPQRSQGTLMCHYRHHA
                     HADPFFLPGLQDITSHVDFSAIARAGVEAGLSLLGYTSQAQFLINCSITEILAEVPPD
                     DAAAYLPLASAAQKLLSPAEMGELFKVLALGKGVTEKLLGFASGDKSSVLS"
     gene            complement(15984..16193)
                     /locus_tag="VLL03_05220"
     CDS             complement(15984..16193)
                     /locus_tag="VLL03_05220"
                     /inference="COORDINATES: protein motif:HMM:NF025733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HSS46797.1"
                     /translation="MDKPNKHCAKCGTAFVCDYALGKQTCWCSQNFPAVMPMKNSELG
                     CYCPVCLEKKISALMLEASAKRGAG"
     gene            complement(16201..16860)
                     /locus_tag="VLL03_05225"
     CDS             complement(16201..16860)
                     /locus_tag="VLL03_05225"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HSS46798.1"
                     /translation="MKIILSWSGGKDSMLMLHKLLQEGEREILALITTVIEGEGKIVM
                     HEVPQALVEAQACALGFPLYTLALPRNPGNAVYEEKFGGMLERLKRRGVEGIAFGDIF
                     LEDIRDYRQGFLQKVGVQPLFPIWKTDSHRLARDFIELGFKGITVCVDSRTLDASFAG
                     REINEDFLQSLPAGVDPCGENGEFHSFVYDGPLFKDRVHFKKSQINQRDIFYYCDLTA
                     Q"
     gene            complement(16948..>17507)
                     /locus_tag="VLL03_05230"
     CDS             complement(16948..>17507)
                     /locus_tag="VLL03_05230"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSS46799.1"
                     /translation="AELNLAAPGIETNKLGFIKVNNKLETNVPGIYAIGDVKGGPAFT
                     HISYDDFRILRANWLEGGNATVDGRLVPSTMFIDPQLASVGLSETEARAQKRNFRVAK
                     LPMTHVARAIEMGETRGFMKAIVDADTGRILGCTVLGVEGGEIMAVLQMAMMGGVHYA
                     QIKEGIFAHPTLAESLNNLFMAMDA"
BASE COUNT         3760 a         5197 c         4984 g         3566 t
ORIGIN      
        1 cgccgcgcgc tcggaaaatt tggcggtcgg cgcgaatttc ggcggctcga gaataatcag
       61 atcgaagcgt ttgccccggt cgcgaagggc gcgcaaaact tcaaacacat caccttcgcg
      121 caattcaaca ctttcccggg tcaattggtt aagctcgaga ttttgccgcg ccagcataag
      181 cgcatctgct gaagcatcca cagatatcac cgatttcgcg ccgccggcaa gcgcatggat
      241 gctgaagccg ccactatagc aaaaacagtc cagcacttcc ttgccgtccg caagttcgcc
      301 tatgcgcgta cggttgccgc gctgatcgag gtaaaaaccc gttttctgcc ctgcctgcac
      361 gttgacctgg aattgcaacc gcccttctct gattttgatg acttcagacg gcggcgagcc
      421 gcgcaacaca cccatgcgtg ggggcaagcc ttccagttgc ctgacttcgg catccgaacg
      481 ctcgaagact ctcgccgcgc cggtcaattc gatcagaata tcggcgcagg tctcgcgcca
      541 gcgctcggcg cccgcgctca aggcttgcaa caccaatgtg tcgccgtaac ggtcgacaat
      601 caagccgggc aaaccatccg attcgccatg caccagccgc agggcatcgg tatcctgaat
      661 cagttttttt cgcgtctcaa cggcctgggc taaacgggaa cgaaaaaacc cggcatcaat
      721 gcggccttgc ttttgccaat cccacacccg tgccacgatt tgcgagtgag gactgaaggc
      781 ggcatgggcg agaaattccc cgttggatga aacaatttct acggtatcgc ccgcttgtgg
      841 cgaatcttga attttttcca ccgcgccgga gaaaatccag gggtgacggc gcagcagcga
      901 tttctcgcgt ctcggcttga gaataagttc gttcattgat tgttgtgagc cgccatcgcc
      961 aaggtacttc agttcgtcat tctgagggtg aaacccgaag aatctgtttt ttgattcaac
     1021 aagccgatcc ttcgccgcgc tcaggatgac aagggtacat tttcttggca tcctgagcgg
     1081 ttcagcggtt tcacggtttc ttcttggcgc ggggatgcgc cttgtcgtag gctttggcca
     1141 ggtactggaa atccaaatgc gtataaatct gcgtggtgga aatgctggcg tgccccagca
     1201 tctcctgtac cgcgcgcaaa tcgccgcttg attgcaatag atgcgaggca aacgagtggc
     1261 gcagcatgtg cggatgaaca tggctgctga tcccctgctt caccgcccat tttttgacga
     1321 gatactcgat ggcgcgggtg cttatgggct taccgtccct gttcacaaac aacgcatcgc
     1381 ttctggcggt tttgtcccgc tcgctcagcc aggcgcgaat cgcgttgagc gcgtgactac
     1441 ccaccggcac gatgcgggtt ttgctcccct tgccggtgac gcgcaccgtg gcattgtcga
     1501 gatttatatc cggcactagc aatcccgcca gttcgctcaa gcgcagaccc gaggaataca
     1561 acaattcaag tatcgccttg tcgcgcaacg cctgcgggcc gtccgttgag atttccatca
     1621 gccgattcgc ttcgtcggga gacagcgcgt gcggtagggt cttggccgat ttcggagcgc
     1681 gcaagccctg gcaggggtta tgcgtaaagc cgctatcgcg cgcaagataa cggtagaacc
     1741 cgcgccaggc ggagagcatg cgcgcgagcg tcttgccgct caagccctgc gcgtgtagtt
     1801 gcgccacaaa acggcggata tgttggatct gcaagtcctt gagaagcgtt gtgccggcga
     1861 gttgcaacag catcaccaca tcgcgtgcgt agttttcgca ggtatggcgg ctcaagcgtc
     1921 gctcagtggc gagatacgaa agatagcgcg cgagatgggc attgcgcgaa ctccgcgcaa
     1981 tactaccttc cgtgctttta ctcctcactc ctgactcttc acgtcagatg gcgcaggagt
     2041 gccgcactgg caagctcgcc gagacgcttc agatacaagg ttcccatctc cgggtagaag
     2101 cgctgcgcgt cctcgctcgc cagcacaagc aggccgaagg tgcgctcgct gcgcagcgcc
     2161 accagcgcga atgaacgcag ccttgtggcg tcttcgccaa accagtctgt gcccccaaac
     2221 tgcgcgccgc aatggggatt gatgagggct tcggcagagg cgcgcagttc ttccttgact
     2281 tcgccgaatt ccggcagtgc ttgcgggttg ccgttgacgc cccacagccg cagcatcacg
     2341 tgcggtacgg caaaatcctc gcgcaagttg aaatacagcg agtgcaaaag gccttccaga
     2401 ttgcgcgcgc cgagcagtgc cagcgccatg cggtggacgc gctcgccgat ggcatcgttt
     2461 tcctcgccga attggatcaa ctccccgagc ttgttttcga tctggcggtt gcgctcgcgc
     2521 agcgtgagaa tctgccgttc gctgatggga atcgcccggc cgccatgcgg gtgtggaatg
     2581 taaatgttgg cgagtaaatc cgcgtaatcc tcgaaaaatt ccggatgggc ctcaagatag
     2641 gccgctacat cttccgcttt tattttcatc cttgcccctt tttagatttt gattatgcct
     2701 tcgtaaaccg tggtcgcgtc gccggtcatc cataccggct taccctcgcc ttcccagcag
     2761 atagtcaatt cccctccctg cgtttgcacc actacacgct tatcaagcag gtcaagctgg
     2821 atgcccgccg ccaccgcggc acaggcgcca gtgccgcagg cgagcgtctc ccccaccccg
     2881 cgctcataga ccctgagctt gatgcggccg cgatccacaa cctgcatata acctgcgttt
     2941 actccctgcg gaaagcgcgc gtgcttttca atcagcgggc cttgggcggt caccggcgcc
     3001 ccgtctacgt ccttgaccac ttgcacggcg tgaggatttc ccatggagac ggcgcttatt
     3061 ttaatctgtc gtcccttaac gattatagaa taagtgagcg cacgcttttt tgcttcaaaa
     3121 ggaatctttt caggcgcaaa acgcggtacg cccatgttga ctgtgacttg gccgtcagct
     3181 tcaaggcggg ggacgataat tccgcccttg gtttccacgc ggatttccgt tttattggtg
     3241 agccccttgt cgcgaacgaa acgcacgaaa caacgggcgc cgttaccgca ttgctcaact
     3301 tcgctgccgt cggcattaaa aatccggtag cgaaaatcgg cgtctgagcg tgcggttttt
     3361 tccaccacca gaatctggtc acagcctatt ccaaaatgcc gatctgcgat tgcgcggtat
     3421 tggtttgagg tcagtatgat gcgttgggaa atcccgttta aaacaacgaa atcgttgccg
     3481 aatccgtgca ttttagtgaa ttttagaagc atttagctgc aaaataactg taaatcacag
     3541 aagttgacgt aattttcgta acagattgat tccttgagaa aacatagtct gccatagccc
     3601 ccggggtgcg tcgaggttat atctcgaaag tcaagattcc aatgtttctt ggggttagta
     3661 aggcttcggc tcccctgggg ggcgggtttt gaagcgcctg tgcaaccagt aatactgttc
     3721 cggcatttcc agcacccgtt cttcgataaa gacgttcatg cgtcgcgtgt cggcttcggt
     3781 attatcgcct ggaaaatcat cccaggcagg gtagaactta agcacataac ccgcaccacc
     3841 tggaagctgt cgggtcacac aaggcacgat tttcgctcct gtcatgcgcg cgagccgcga
     3901 caagccggga atggttgcgg cggagacgcc gaaaaaaggc acgaacaggg aatcgcgggg
     3961 cccgaaatcc atgtccggca aatagtaaaa cggcacgccc tcacgcaatg cgttgataat
     4021 gggacggatg ccttcttgcc gcgataccag tatcgcggtg ccaaagcgtg tgcgcccgtg
     4081 caggagtagg ccattcacca gcgaattctt ctgctggctg tacatcgaca caaggggata
     4141 ttcgctggtg atgcgcacgc cacccatgtc gagtcccacg aagtgcggcg caagccagat
     4201 taccggctgg ttgcgcaccg cttgccagtt ttccagacct tcgatgtgca ccagtctttc
     4261 aatgcgttca cgcgatgacc accacaaaat accgtgttcc agaaaattgc ggccaaaggc
     4321 gcggaaatgc tggcgcaata tgttctcgcg cttttcatcg ctccactgcg gaaaacacag
     4381 ctgtagattg gtgcgagcca cgtggcggcg ttcgtggccg aacacaaaca gcagcatgcc
     4441 catccattcg ccgagcttgg ccaaaacacc aagcggcaaa aaatgcagca gccacaccag
     4501 cacgaggcca atgcggacca tcatgcgcac ctccaaataa aacccggcgg ccactcaaat
     4561 aacacccgtt ccagcatggc tttagaggga aaacggcttt cgttattggg gctaatttgg
     4621 tatcctaaac ggtcttttat cagccgcact cgaggttatc atgaacgaat acctgtttac
     4681 ttccgaatcg gtttcggaag ggcacccgga caaggtcgcg gatcagattt ccgacgctat
     4741 tctggacgct attctagcgc aagacaaaac ctgcagagtc gccgcagaaa ctctgtgcaa
     4801 caccgggctg gtggtgctgg cgggggaaat caccacgaac gccaacgtcg actacatcgg
     4861 cgtagcgcgc gagaccatca ggcgcattgg ttacgacaac accgagtacg gcattgacta
     4921 caagggctgc gcggtgatgg tctgctacga caagcagtcc cccgatatca agcaaggtgt
     4981 ggacaaagcg caggatgatc ctctcaacca gggtgccggc gaccaggggc tgatgttcgg
     5041 gtatgcctgc gacgagactc cgcagctgat gccgctgccg atcttcctcg cgcaccgcgt
     5101 ggtggagcgc cagtcggaga tccgccgcga cggacgcctg ccgtggctac gccccgatgc
     5161 caagtcgcag gtcaccatcc gctacgtgga tggcaaaccg aaagaaatcg acactatcgt
     5221 gctgtcgacc cagcacgctc cggacatcgg gcacgacaag ctcaaggaag cggtaatcga
     5281 ggaaatcatc aaaccggtgc tgccaaaaaa catggtcggc gccgacatca agtatctggt
     5341 caatcctacc ggcaaattcg tggtcggcgg cccgcatggc gactgcggcc tgaccggccg
     5401 caaaatcatc gttgatactt acggcggcgc ggcgcctcac gggggcggcg cgttttccgg
     5461 caaagatccg tccaaggtgg accgttccgc cacttacgcc gcgcgctatg tggcgaagaa
     5521 catcgttgcc gccaggctcg cctccaaatg cctggtgcaa gtcgcttacg cgattggcgt
     5581 ggcgcgcccg gtatcgctga tggtcaatac cttcggcacc ggcaaaatta gcgacgacaa
     5641 gatcgtaaaa ctcatcgaag tgcatttcga cctgcggccc aaaggcatca tccagtcgct
     5701 caatctgctg cgaccgattt atctcaagac cgcatcctac ggacacttcg gccgcgacga
     5761 gcctgaattt acgtgggaga acaccgataa ggcgatggcg cttgccgaca ccgccggtat
     5821 gaaacgcgaa accatgatcg cctagttcga acacctgccg ttctgagggg cgctgcagcg
     5881 gggtttatat ccgccaggct cggaataggg ggtatctaaa gttgcaacgg cgcccattca
     5941 gattttttga atggagaaac attatgagcg ctgtactcaa atcccaacag gactacaaag
     6001 tcgcggatat ttccctggcg gactggggcc gcaaggaaat cgcgatcgcc gaaaccgaaa
     6061 tgcccggctt aatgagcgtg cgcgaggaat accgcgcgca gcagccgttg cagggcgcgc
     6121 gcatcgccgg ttcgctgcac atgaccatcc agaccgcggt gctgattgaa acgctgaaag
     6181 cactgggtgc ggaggtgcgc tgggcttcct gcaatatcta ctcgacgcaa gatcacgcgg
     6241 cggcggcgat tgcggcgggc ggcacgccgg tgttcgccta caagggcgaa acgctggatg
     6301 aatactggga gtacacccac cgcattttcg agtgggcgga cggcggcttc cccaatatga
     6361 ttctcgacga tggcggcgat gccacgctgc tggtcatcct gggctcaaag gcggaaaaag
     6421 acccgacggt gattgccaaa tcggccaacg aggaagaaaa ggcactctac gcttcaatca
     6481 aaaagcgcct cgacaccaag ccgggctggt attccaaagc actggcgaat atcaagggcg
     6541 tgaccgagga aaccaccact ggcgtcaagc gcctttatca gatgcagaag gaggggcggc
     6601 tgccgtttcc cgccatcaac gtcaacgatt cagtcaccaa gtccaagttc gacaaccttt
     6661 acggctgccg cgaatcgctg gtggacggca tcaagcgcgc gaccgacgtg atgatcgccg
     6721 gcaaaatcgc cgtggtgtgc ggccatggcg acgtaggcaa gggctgcgcg cagtctttac
     6781 gcggcctcgg cgcaacggtg tgggtcaccg aaatcgaccc catctgcgcg ctgcaggcgg
     6841 cgatggaagg ctatcgcgtg gtgacgatgg aggacgcagc cgacaaggcg gatatttttg
     6901 taaccgccac cggcaatttc catgtcatcg ccctcgagca catgaagaaa atgaaacata
     6961 acactatcgt gtgcaacatc ggccattttg attcggaaat cgacatcgca tcgcttcgcc
     7021 agtaccggtg ggagaatatc aaaccgcaag tagaccatgt gattttcccc gacggcaagc
     7081 gccttatcgt acttgccgag ggacgcctgg tgaacctggg ctgcgccacg ggacatcctt
     7141 cattcgtgat gtccaattcc ttcaccaatc aggtcattgc gcagatcgag ctctatcagc
     7201 atggcgccaa atacgagaag aaggtgtacg tgctgcccaa gcacctcgac gaaaaagtgg
     7261 cgtggctgca cttgaagaaa atcggggcca agcttaccga gctcaccgac gaacaggcaa
     7321 aatacatcgg tgtgaacaag gcgggcccgt tcaagcccga gcattacaga tattgattca
     7381 tgaccacaga gacattgaag ttttctgtct caatgctccg gtggtaaata aatgcaatca
     7441 caaaaaaaat atccgcgtac atttagtttt gaatttttcc cgccgcaaac gccggaaggg
     7501 gtggaaaagc tgcgcgcaac aagaatacaa ttggcgcaac tcaagcccga attcttttcg
     7561 gtaactttcg gcgccggcgg ctcgacccgc gaccgcacgc tggaaacagt gctggaaatc
     7621 cggcgcgagg gccataacgc cgcgccgcat atttcctgca tcagctcgac gcgccaaaac
     7681 gtccgcgcca ttctcgacca ataccgggaa agcggcatca cccgactggt ggccttgcgc
     7741 ggcgatttgc cctcgggcat ggccgctccg ggccaattcc gctatgccaa cgagctggtg
     7801 gaattcatcc gcaaggaaac cgggcggcgt tttcacatcg aagtcgcctg ttatcccgaa
     7861 tttcacccgc aggcgaaatc ggcacgcgat gacctgctta acttcaagcg caaggtggaa
     7921 gcaggcgccg attcggccat cacccagtat ttcttcaacg ccgacagcta ctacagcttc
     7981 gttgacgaat gcgaggcgct gggaatcaaa attcccatcg tgcccggtgt catgcccatc
     8041 aacaaattca cacagctcgc tcgcttttcc gacaactgcg gggcggaaat cccgcgctgg
     8101 gtgagaaaaa aactggaggg ctttggcgac gacacggctt cgattcgcgc cttggggctc
     8161 gatgtggtca ccaagctctg tgatgacctg ctcgaaagcg gcgcgcccgg tttgcatttt
     8221 tatacactga accaggccgg actcacttcg acaatttggc agcggctggg cttgtagcgg
     8281 aatgcggcgc atcctcggtg accctctcaa cctattggcg cttgccatcg tgctcgccgt
     8341 ggtcgtcgaa ttctcctggc tgaactggtt gcgcacgcca gaggcacgct tctccgatct
     8401 tttcgtcgcc gctcaggcgg agaggctcag gccggattcc gatatagtca tcgtcgccgc
     8461 cgccgaggac agtttggagc ggatggccga ctacgcgggg cgctggccgt ggccgcgctc
     8521 cgtgcatggc gaactggtgc agggcattgc cgcacagaag ccgcgcgcca tcgtattcga
     8581 catcctgttc ttcgagccgg acatctaccg gttggatgcg gacgagttgt tcaacaaagc
     8641 agtctctccc cacaagaacg tgttctttcc gactgcacgc caggatgccg cggcagaccc
     8701 ttatggcgtg ccgctcgcgg aaatacaagg cgcgctcggc gcggtcgccg gtgcgcgggc
     8761 cgaccggaag gccacgttga acatcggcct gcccaaggcg ttgctgccgg agaactggcg
     8821 cctgggcacg atcgattttc ttgccgaccg cgatggcgtc gggcggcggt acttcgtata
     8881 tcaggacgcg tatggctgga agattccttc tcttcccgcc agggtggcgc aggacctcgg
     8941 actagcggtg cctgatgtca agtccatcct gctgagctgg ccgggtggca aggctgcacg
     9001 agcgtacgtc tcctacgccg acctctacgt cgatttcaac agccaaaaac gcaagcgtga
     9061 tccgaacgag ttcaaggaca agatcgtcat cataggcgtc acggcaaccg ggctgcacga
     9121 catccggccc actccggtga acatacgctc ggatggcgtc gatatcctcg ccacggcgat
     9181 cgacaacctc aagaatagaa actacctatc gcagatatcg gtcgcctggc cggcggcttt
     9241 gaccttgatc atgctggcgt tgctgtatgc gggatttcgc gcccgattca atacgctgaa
     9301 gatcggcgcg gcgctggcgg gggcttcggc cgttctgcta atcgggcagt acatcgcagt
     9361 cagcaagttg ctgctggtgc cggtgctgcg gccgctgctg ttcggctggg cgttctattt
     9421 catcaccgct ttgcaggaat atctgcgcga acggcgcggg cgcgagcagg caatcgcgca
     9481 gttttcccgc ttcgtcaatc cgcacgtggt gaaggagctg attgcgcacg gcgggctgtc
     9541 gcgcgaaggt cagaaccggc aggtgacgct gctgttctcc gacatccgca gctttaccgc
     9601 cttgtcggaa tcccgcaccc cccaacaagt tgtggatctg ctcaaccgct acttcagccg
     9661 tcaggtggac gtgatcttcc gccacggcgg tgcgctcgac aaattcatcg gcgacgccat
     9721 catggcgatc tggggcgcgc cgctcgacga cccgaagcac gccgaaaatg cgattcgctg
     9781 cgcgctggac atggcagaca cgctcgaggc attccggaag gaactcggcg acatgggtgg
     9841 cgaattcgat gtcggcattg gcatccactc gggctcggcg gtggtgggcc taatcggttc
     9901 cgaccagcgg cgtgagtaca ccgccatcgg cgacacggtc aacctggcga gccgcatcga
     9961 agggctgacc aagggcgtgg cgagaatcct agtttccgaa gacaccctgc ggctgtgccc
    10021 ggacgccttt gactttgttc cacgcggacg ctataaagtc aaaggcagaa cgcaggaggt
    10081 cgagttgttc gaaccgaaga gaaaatccgc atgaaacgct gggcatggct cgccacggta
    10141 cttaccctgt tgatcccgcc ggcacacgca gccgaaaccg gctacacgct gggcccaacc
    10201 gaattgaagg caaagccttt tctagacgcg gaaacggtga cgacgcttcc tgagaaatcc
    10261 caggttgaga tcctgacccg ccagggcgcc tggatgcaga tcaagaccaa ggaaaagcag
    10321 cagggctggg tgcgcatgct gtcggtgcgc ctcggcaatc cggaccagaa accccagggc
    10381 ggcaatctat tgtcggccat tggattctcc aaccgaccgc gaccggtgac gaccgcgacg
    10441 gtgaccactg gcgtgcgcgg tttctccgag gaggatctcg ccaaggccgt gccgaatccc
    10501 gctgaagtgg taaagatgaa gggttttgcc gcgaccccgg ctaacgcgca gcaactggcc
    10561 gccaagggca agctggggca acgccaggtc gcctacttcg acgaaaacgg caagccgctg
    10621 aaggagataa aatgagctcg ttgcgctttg ctttcattct gctcctgtcg gcgttggcgt
    10681 ccggcggctg ctcgcaacaa caaacccagg ccttctttcg caacatcaac gagtcgggcg
    10741 gtctacagca atcgctcaag agcctgcgcg agccgacgca ggccgaggag atcgaaatcg
    10801 gcgacggcat tgccgagacg ctgctcggtg cccgcccgct gttcaatgat gcggagctgc
    10861 agcgctatgt gaatgccgtc ggcatgtggg tggcacagca gtcggaacgg ccggacctgc
    10921 cttggcattt cggcgtcaac gattcggacc acatcaatgc gtttgctgcg ccgggcggtt
    10981 acatcattgt caccaaaggc atgatgaaac agttgcgcaa cgaagccgag cttgccggcg
    11041 tgctcggtca cgaagtggcg cacgtgacgc agaagcatca tctcaaggcg ttgcgcaaat
    11101 ccgccgtgat gaatctgctg agcgcggggg tggcggcggc taccgctgaa agccggcacg
    11161 ccgatctggt gcagaagttg gcgggtccga ccaaggagct ctacgcccgc ggtctcgaca
    11221 agggcgacga atacgaagcc gaccgcatgg gagtggtgct ggctgcgcgg gcgggctacg
    11281 atccgtatgg attgccggcg gtgctgacca cgctcgccag cgccgatccc agggataatt
    11341 tcctaacgct gctgtacaag acccacccgc tgccccagga ccggctcgac aagctcgcgc
    11401 ccggtttggc cacgctcgac ggcatcaatg cgccgcagaa catgatgcgc ttccagcagt
    11461 acaccaagaa gttacatctc tcctcgcagt aattccccgg gattgcgaat gggaagccga
    11521 gtttccatcc tgatcctgct cgcggccttc tgttttcctg cgcaggccgc gcactttgcc
    11581 ggcacgcttg agcccgcggg cggcgggcgc aaataaaagc atcgcgcgca gcgtgagccg
    11641 aaattcaaaa aaagcgaaat ccggcgcggt gcggcgcgcg ctccgtccgg acctcgacct
    11701 gagccaacgg gaatttgaca cgctcaaacg gctgaggacg ccgcacaaaa tccaggaata
    11761 tttaaacggc atcaaggcca acttcgaacc gcacggcgac actgtcctgt cggtgcgcga
    11821 agtgctgaaa cagcgccgcg cacattgcat cgagggagcg ttgctcgccg cctgtgcgct
    11881 gtggatccaa ggcgagccgc cgctgctcct cgatctcaag gccgtgcacg actacgatca
    11941 cgtggtggcg ctgtttcgcc gcggcgggtg ctggggcgcg atttccaaga ccaaccatcc
    12001 ccagctgcgc caccgcgacg ccgtgtaccg cagcttgcgg gaactcgcaa tatcctattt
    12061 tcacgaatac accaacaaga aaggccgcca gacactgcgg cagtattcgc gcgccttcga
    12121 tctgcgcaag gtggatccca aactgtgggt gacgaatggc gacgattgct gggaagtggc
    12181 ggatattctg gatgaaatcc atcactaccc gctcataacc gcaaagcaga cgcggctcct
    12241 gcaccgccgc gatgcaatag agcgcaaggc cggaaagctg ctccagtatc cagcgccgcg
    12301 gcggaaatag cgcctatgct tgcattggaa gcgaaattga ctccgcctcc attttttaga
    12361 gcttaaaccg ccatggatgc ggaagacgca gagagaatca aattctcata ttacaagacc
    12421 tgatcccggg tttttctgtt acttgttact tgagcggcgt gggcatgacg gacgagtgat
    12481 gatcgatgat cagccatttt tttcccttct tcacgtaggt aaagctgtag cgtgagggga
    12541 gagacttggc ctcgccgtct tttacataag tgaacgtgta atagccggtg ttgatggcga
    12601 tgttgccgta tacccggatt aactggtcac cgaaactgac tttgtggctc gggagagcca
    12661 cataggcatt cacaaaataa tcccgaaccg cttccggatt atcccgccgt ttgggagaca
    12721 gcgttcccca cagcacggcg tctttggcat agagcgcgag gatcgtgtca gggttgtcgt
    12781 ccgtgaaaag ctccgcccat ttcgcggtgg cggcagcaac ctcatccttg ggccctgccc
    12841 aagcgctggg tacaattaca aacagggtaa gcaacagcaa agagtagcta tggcgtttca
    12901 tgattaacct cccttggttt ggttattggc ccgctgtggc cgaagtgcgg ctaaggtaac
    12961 cttttttgga tataggctgg atcgatggcg gagtcaagca ctatccattt gataaaaagc
    13021 ccgaattgtt tctaaaccct cggtaaattg ataccaaacc ccattttcta ccgcacccaa
    13081 taataagcgc ataaacgagg cgctaatcat cccctggtaa taccgtgagc agcgggcgca
    13141 tggcagtaag cagactcggg aagagctggc ggttttcgcc ttcccattgg gccagctttt
    13201 gcttgatgtt ccagcgctgc atcggcatgg cgaaacaggc cgggcaattc tccgcaatca
    13261 ccggcagccc ggtgcttttg gcaaaagcat tggtttgccg ttcgcgcacg taaacgaacg
    13321 ggcgtaccac ccgcagatcg ccgtcgtcgt tgacataatg cgcgcgcatg gtgcgcagcc
    13381 tcccgccgaa catcgcgctc atcatgaagg tctcggcgaa atcatccaga tgctgcgcca
    13441 gcgccagtac gttgcagcct tcgcgccgcg cggcgccgta gagcatgccg cggcgcaggc
    13501 gcgagcaaaa tgcgcagaac gaagcgttgc ccattttttc cttggcgagc tccagcaccg
    13561 ggtagctttc atagaaatac ggcacgccca gcccggccat gtaggccttg agcggctcgg
    13621 gatggtattc cggcgattgc ggatctacag tcgcggcggc aagctcaaac ttcaccggcg
    13681 atttcgcctt aagatgtaat aacacatgca gcaaggacaa actgtccttg ccgccggaca
    13741 ggccgagcaa tacacggtcg cccggccgaa tcatcgcgta atcggcaatc gccttgccgg
    13801 cgaggcttaa taaagtgcgc gggggtttaa tcgggcttgt tttcatattt aattccgtaa
    13861 gttcacaatt gaggatgttc ggattttagc gaaacaagaa gtttagcttt agacgaggcg
    13921 cggcgaagtt gagcagcgga gccggtaagt atgtgagcag cggaaacgag ccgcaacgaa
    13981 atataaaggc aaaatccgcc gcttcgctca tatgttgata ctcctgccgc catcgacggc
    14041 aataatctga cccgtaatat aaggcgcatc gtcaatcagg aaatataccg ccttggcgat
    14101 gtcttccggg tcgccgactc gcttcaacaa cgttgaatgg ataatgcgct ggcgcgagac
    14161 ttcatccgac catgcctcgt cctccggcca aacgatggcg ccgggcgcga cgccgttcac
    14221 tctgacttcc ggcgcgagct cccgcgccag cgagcgcgta aggtttacca gcccggcttt
    14281 cgccgtgctg tacaccacgt agttcttcat cggccgctca gcgtggatgt ccacgatgtt
    14341 gacgatgcag ccgcggtttt ttttgagttg cgccaccgcc gcctgcgaca gaaacagcgg
    14401 cgccttgagg ttggtgccaa ccaaatcctc ccacaccttg tcgctgattt ccccgaccgg
    14461 cgtcgggaaa aagctcgaag cgttgttaat cagcacatcc agcctgccaa agcgcttcac
    14521 cgtgtcgttc accaggttcg gcagccctga cagatggagc aaatccgcct gaatcaatgc
    14581 caccgaatcg gcgcgctgca aattgagctc ggaggcgagc gagcgcgctt ccttcgacga
    14641 agtgcggtaa tgcaccatga tgccggcgcc acgcgcatgc agatggcggc aaatcgcggc
    14701 gcccacgcgc ctcgcgccgc cggtaatcag caccactttg ccttgcaccc cgtttctccg
    14761 ctatattgca gtaaagctga aattttacag caattctgac ggcgtctccg ccatacgcgc
    14821 tttcaaccga tgccacttcc tccgcccagc gcagaagcgc agcagcatag tgaccagctt
    14881 tgccggctga ttcgcggtga aatcgccgcg aatggcggca ggatttcctt tgcccgctac
    14941 atggagctcg cactttacgc gccggggctg ggctattaca acgccggggc gcgcaagctc
    15001 ggagccggcg gtgacttcct cactgcgccc gaactctcgc cgctgttcgg ccggacgctg
    15061 gcgcagcaag caaagcagat actcgagcgg ggttacgacc agatcctgga aatcggggcg
    15121 ggcagtggcg cgctcgccgc cgccctgctg gaagagttgg agcggctcgg acgcctgccg
    15181 cgccattacc atatcctgga aatcagtccc gatttgcgta gccgcagccg cgacaggctt
    15241 gcggcgcacg tgccgcatct gttggagcgt gtcgcatggt tgaaccagct tccacccgcg
    15301 ttctcgggtc tcatcatcgg caatgaagtt ctcgacgcaa tgccggtgaa tgtcgtcttg
    15361 atgcgtaagc gcgacatact tgaagccgat gtccaatatg acacagacaa aaacggattt
    15421 gactggcacg aaacgcgcgc gcccgatccc ttgcgcgaag cggcctcgcg gctcaacctc
    15481 cccgcggaca attaccggac cgaaatcgga cttgccgcgc aaggcttctt gcgctcgctg
    15541 gcaagcacct tacaggaggg cgttgcatta ttcatcgact atggcttccc ggcgcgcgag
    15601 tactatcatc cgcagcgcag ccagggcacg ctgatgtgcc actaccggca ccacgcgcac
    15661 gccgacccgt ttttcctgcc cgggctgcag gacatcacca gccacgtcga cttcagcgcc
    15721 atcgcccgtg ccggcgtgga ggcgggcttg agcctgctcg gttacaccag ccaggcgcag
    15781 tttctgatta actgcagcat caccgaaatt cttgccgagg ttccgcccga cgatgctgcg
    15841 gcttacctgc ctctggcttc ggccgcgcaa aagctgttaa gccccgcgga aatgggggag
    15901 ctgtttaaag tgttggcgct cggcaaaggc gttaccgaaa aactactggg ctttgccagc
    15961 ggcgacaaga gctcggtact gagctaaccg gcaccgcgtt tggcggaagc ttccaacatc
    16021 agggcggaga ttttcttctc caggcagacg ggacaatagc aacccaactc ggagttcttc
    16081 atcggcatca ccgccggaaa attctggctg caccagcacg tctgcttgcc cagcgcgtaa
    16141 tcgcagacaa aagcagtgcc gcatttggcg cagtgtttgt tgggtttatc catgggctct
    16201 ttattgcgcg gtgagatcgc aataataaaa gatgtcacgt tgatttattt ggcttttttt
    16261 aaaatgcacg cggtccttga ataacggccc gtcatacaca aagctgtgaa actcgccgtt
    16321 ttctccacag ggatcaaccc cggcggggag gctttgcaga aaatcctcgt tgatttcccg
    16381 gcccgcgaac gaggcatcga gtgtgcgact gtccacgcac acggtgatgc ccttgaatcc
    16441 cagctcgatg aagtcccggg ccagccggtg gctgtcggtt ttccagatgg gaaacaaagg
    16501 ctgtacgccg actttttgca aaaaaccttg ccgatagtcg cggatgtctt ccaggaatat
    16561 gtcaccgaag gcaatgcctt ccacgccacg ccgtttgagc ctctccagca tgccaccgaa
    16621 tttttcttca taaacggcat tgcctggatt gcgtggaaga gcgagggtgt atagcggaaa
    16681 acccagggcg caagcttgcg cttccacaag ggcctgcggc acttcgtgca tgacgatttt
    16741 gccttcgccc tcaatcacgg tggtaatcag cgccagaatc tcgcgttcac cttcctgcag
    16801 cagcttgtgc aacatgagca tgctgtcctt acccccgctc caggacaaaa tgattttcac
    16861 aggcggatga tttgcacgcc gcgcatcagt accctaaacg gtcatccaga gggccatcat
    16921 gtcccggcgc cgctgaaagc gcaagcttta cgcatccatc gccatgaaca agttgttgag
    16981 cgactcggcg agagtggggt gggcaaaaat cccttccttg atttgcgcgt agtgcacccc
    17041 gcccatcatt gccatttgca gcaccgccat gatctcgccg ccttcgacgc ccaatacggt
    17101 gcagccgaga attcgcccgg tgtccgcgtc cacgatggct ttcatgaaac cgcgtgtttc
    17161 tcccatttcg atcgcccgcg ccacgtgggt cattggtagt ttcgcgacgc ggaagttgcg
    17221 cttctgtgcc cgcgcctcgg tttcgctaag ccccacgctt gccagttgcg gatcaatgaa
    17281 catggtgctg ggtaccaggc gcccatccac agtggcattt ccgccttcca gccagttcgc
    17341 gcgcagaatg cggaaatcgt cataggaaat gtgcgtgaac gcggggccgc ctttgacatc
    17401 gccgatggcg tagatgcccg gaacgttggt ctccagtttg ttattgacct tgataaaacc
    17461 cagcttgttg gtctcgatcc ccggcgccgc gagattcaat tccgccg
//
LOCUS       DATIOW010000156         2254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1186047, whole genome shotgun sequence.
ACCESSION   DATIOW010000156 DATIOW010000000
VERSION     DATIOW010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186047"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1323)
                     /gene="gshA"
                     /locus_tag="VLL03_05235"
     CDS             complement(31..1323)
                     /gene="gshA"
                     /locus_tag="VLL03_05235"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HSS46800.1"
                     /translation="MAVPHLTAALPGQVPELEIKLVEKVSAIEHWLRGQWQEHSVPFY
                     ASVDLRNSGIKLAPVDTNLFPGGFNNLNPDFLPLCVQAAMVAVEKVCPDAKGALLIPE
                     NHTRNTFYLQNVATLQSILRQAGMNVRIGSLLPGITAPTELKLPNGGSLLLEPPARKG
                     NRLGLANFDPCVVVLNNDLSAGIPEILKNLEQSVLPPLHAGWATRRKSNHFSAYNRVA
                     EEFAKLIGIDPWLINPYFATCGEINFQEHRGEECLTSWVEQILSDIRAKYQEYGIKEQ
                     PFVIVKADAGTYGMGIMTVKSASDVRDLNRKQRQKMAVVKEGLLVTEVMVQEGVHTFE
                     SINEAVAEPVVYMIDHFVVGGFYRVHTSRGTDENLNSPGMHFVPLAFESPCSLPDPTQ
                     PGCVPNRFYVYGVIARLALVAAAVELEQGAKVFEKAIA"
     gene            complement(1445..>2254)
                     /locus_tag="VLL03_05240"
     CDS             complement(1445..>2254)
                     /locus_tag="VLL03_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HSS46801.1"
                     /translation="QVVVYATIAHWVWEPSGWLAAMGVLDFAGGTVVHINSGIAGLAC
                     ALMLGKRLGYGKEAMPPHNLVLTVIGASLLWVGWFGFNAGSAVAADGRAGMAMAVTQI
                     ATATAALAWMFTEWLVKGKPSVLGIASGAVAGLVAITPASGFVAPIGSLWIGVAAGVF
                     CFFATTTVKNMLGYDDSLDAFGVHCIGGFTGAILTGVFAVKDIGGTAGVLEGNFGQLL
                     IQFIGASSTLIFSFVMSFIILKVLDMVIGIRVTEDQEREGLDIALHGEQVL"
BASE COUNT          504 a          665 c          628 g          457 t
ORIGIN      
        1 gaggataaaa gcgaatttca tggggcggcg ctaggcgatc gctttctcga aaacctttgc
       61 accttgctca agctcaacgg cggcggcgac caaggcgagg cgcgcaatca cgccgtaaac
      121 ataaaagcgg ttgggcacgc agccgggctg tgtcggatcg ggcagcgagc aggggctctc
      181 gaatgccaaa ggtacaaaat gcattccggg cgagttgagg ttttcgtccg tgccgcggct
      241 ggtatgcacg cggtaaaacc cgcccaccac gaaatgatca atcatgtaca ctaccggctc
      301 ggccactgct tcgttgatgc tttcgaaggt gtgcacgcct tcctgcacca ttacttcggt
      361 aactagcaag ccttccttca ccaccgccat cttctggcgt tgcttgcggt tgagatcccg
      421 cacatcggac gcactcttca ccgtcatgat gcccatgccg tacgtgccgg cgtcggcctt
      481 aacgatgaca aaaggctgtt ccttgatgcc gtattcctgg tatttggcgc ggatgtcgct
      541 caggatttgc tccacccaac tggttaggca ttcctcgccg cggtgctctt ggaaattgat
      601 ttcaccgcag gtcgcaaaat aggggttgat tagccaagga tcaattccga tgagtttggc
      661 gaattcctcg gcgactcggt tgtaagcgct gaagtggttc gatttgcgcc gcgtcgccca
      721 gccggcgtgc aagggaggta gcaccgattg ttccaggttt tttaaaattt ccggaatgcc
      781 cgctgacaag tcgttattca aaaccaccac gcaagggtcg aaattggcaa gccccaggcg
      841 gttacctttg cgcgctggcg gttccagcag gagacttcca ccatttggca gcttcaattc
      901 cgtgggcgcg gtgattccag gcagcaagct gccgatgcgc acattcattc ccgcctggcg
      961 caggatggat tgcagggtcg cgacattttg cagataaaac gtattacgcg tgtgattttc
     1021 agggatcagc aacgcgcctt ttgcgtcggg acagactttt tccaccgcga ccatcgccgc
     1081 ctgcacgcac aacggcagga aatccgggtt aaggttgttg aatccccccg gaaacaaatt
     1141 ggtatccacc ggcgcgagct tgattccgct gttgcgcaaa tccaccgagg cataaaacgg
     1201 cacgctgtgc tcctgccatt gtccgcgcag ccagtgctca atggcggaaa ccttctccac
     1261 cagttttatt tccagttcgg gcacttgccc gggaagcgca gcggttaaat ggggaacggc
     1321 catggtagaa gaaatgaaag gagaactgct attgtagcgg aaagcaaatc cgcgcgtgtc
     1381 aggaaacgca ggaaaaaaag ggggcgcttg cgcgcccctt caggaggact ttttgttatg
     1441 acgtttacag gacttgctcg ccgtgcaggg cgatatccaa cccttcgcgt tcctggtctt
     1501 cggtgaccct tataccaatc accatatcga gcaccttgag gatgatgaag ctcatgacga
     1561 agctgaaaat gagagtcgaa gaagcgccga tgaactggat gagcaactgc ccgaaatttc
     1621 cttccagcac gccggcggtg ccgccgatgt ccttcactgc gaacacgccg gtgagaatcg
     1681 cgcccgtaaa gccgcctata cagtgcaccc cgaatgcatc cagcgagtca tcgtagccga
     1741 gcatattttt gaccgtggtc gtagcaaaga agcagaacac gcctgctgcg acgccgatcc
     1801 aaagcgagcc gatcggcgcg acaaacccgg atgccggtgt gatggccacc aatcctgcta
     1861 ctgcgccgga agcaataccc agtacgctgg gtttgccttt gacaagccac tccgtgaaca
     1921 tccaggcgag cgcggctgtg gcggtcgcaa tctgggtcac cgccatcgcc atgccggcgc
     1981 ggccatcagc cgccactgcg gaaccggcgt tgaagccgaa ccaacccacc cacagcaaag
     2041 atgcaccgat gacggtgagc accagattat gcggcggcat ggcttccttg ccataaccca
     2101 ggcgtttgcc caacatcagt gcgcaggcca agcccgcgat accagaattg atatgcacca
     2161 ccgtgccgcc cgcgaagtcg agcacgccca tggcggcgag ccagcccgaa ggctcccata
     2221 cccagtgcgc aatggtcgca tacaccacca cctg
//
LOCUS       DATIOW010000157         8487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_474986, whole genome shotgun sequence.
ACCESSION   DATIOW010000157 DATIOW010000000
VERSION     DATIOW010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8487
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474986"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /gene="glyS"
                     /locus_tag="VLL03_05245"
     CDS             <1..1268
                     /gene="glyS"
                     /locus_tag="VLL03_05245"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HSS46802.1"
                     /translation="LSLPQECLILSMKQHQRYFPLANKVGKLLPCFLIVSNLQTTSPK
                     NIIHGNERVLRARLSDAKFFYEHDQKTKLVDRVPKLVDIVYHNKLDNQLARVKRLQKL
                     ASSIAREIRTDVAHAGRAAYLCKADLLTDMVGEFPELQGIMGRYYALHDGEPEEVADA
                     IGAHYRPRFAGDTTPDGRIASAVALADKLDTLAGLFGIGQVPTGDKDPFGLRRAALGV
                     IRILIERDLPLSLHELVNAAFAGYNGRVGDAHTDLESFIFDRLAGYLRDRGYSTLEVE
                     SVVSLRPDQINLVPSQVEAVRAFNALPEAQSLAAANKRVVNILKQAEANGESFVNAEA
                     DKLQEPAERALFDALKTTSRQAASLFNQGNFTGYLKTFAILKSPVDAFFDSVMVMADA
                     PELRHNRLALLADLRKEMNRVADISKLAV"
     gene            1270..1803
                     /gene="gmhB"
                     /locus_tag="VLL03_05250"
     CDS             1270..1803
                     /gene="gmhB"
                     /locus_tag="VLL03_05250"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HSS46803.1"
                     /translation="MKLVILDRDGVINHDSAQYIKSPDEWKPIAGSLEAIARLNQAGY
                     RVVVVTNQSGIGRGLFDMTALNAIHDKMHKSLTQYGGRIDAIFFCPHAQELSCACRKP
                     KTGLFQEIAQRFNVNLAGVPCIGDSARDLQAAHAAGAQPILVLTGKGKKTREAGNLPE
                     STQIYADLAEAAQALAA"
     gene            1807..2523
                     /locus_tag="VLL03_05255"
     CDS             1807..2523
                     /locus_tag="VLL03_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSS46804.1"
                     /translation="MVVLRSLFFALLQAVVTPLFALIALLTFPFDPFTRYHIITTWSR
                     IIIGTARAICGIRYRIVGRENIPDHPCIIMSKHQSAWETLAFQVIFPPQALVIKRELL
                     RIPFFGWGLAMLSPIAIDRDAGTRALRQIEEQGIARLQQGFWVIVFPEGTRIKPGEKG
                     VYQPGGAWLATHSNSMVLPIALNSGEFWRRNAFLKRPGTITVSIGPPIRAQGMKPGEL
                     NAKVETWIESEMARIAKHEV"
     gene            2593..2751
                     /locus_tag="VLL03_05260"
     CDS             2593..2751
                     /locus_tag="VLL03_05260"
                     /inference="COORDINATES: protein motif:HMM:NF025012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HSS46805.1"
                     /translation="MRAFVLLIVLAVLMQGCGYKGPLYLPPQQPAQQPTPPPQKQDAN
                     KQQGSPNP"
     gene            2748..3992
                     /gene="lysA"
                     /locus_tag="VLL03_05265"
     CDS             2748..3992
                     /gene="lysA"
                     /locus_tag="VLL03_05265"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HSS46806.1"
                     /translation="MTYFSYRNKQLHAEAVALAHVAAQFGTPCYIYSKAALTAAYQSF
                     AQALAKREHLICYAVKANSNLAILNLFARLGSGFDIVSGGELARVLAASGDPRKVVFS
                     GVGKSAEEMRAALNAGILCFNVESEAELVRLDRVAGETGKKAPVSLRINPDVNPKTHP
                     YIATGLKQNKFGVDYADALALYRKAGSMSHLQITGIDCHIGSQLTEVAPFTDALEKIL
                     LLIDKLEKTGVRIAHIDIGGGLGIRYRDETPPNGAEYAAALFAKLGRRTQTILLEPGR
                     ALVGNAGLLLTRVEYLKHNRDHNFAIVDAAMNDLVRPALYDAYHDILPVMQSQRSKRT
                     YEIAGPVCESGDFLGRGRKLALAEGDLLAVMSAGAYGMSMSSNYNTRPRAAEVMVDGD
                     QAILIRGRESANQLFAGEKLLP"
     gene            4466..4621
                     /locus_tag="VLL03_05270"
     CDS             4466..4621
                     /locus_tag="VLL03_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46807.1"
                     /translation="MKSSRKTPLKRRNRVALSPLLRKGGAHALQNKKAKRALQKARLR
                     KTLAEEL"
     gene            complement(4618..5196)
                     /locus_tag="VLL03_05275"
     CDS             complement(4618..5196)
                     /locus_tag="VLL03_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HSS46808.1"
                     /translation="MACLVPLARLAWRGFNHDLGANPIEVVTHTTGDWTLIFLLITLS
                     VTPLRRLLEWPWLIKLRRMFGLYAFFYACLHFLTYIWLDQFFDIFEIGKDVLKRPFIT
                     LGFTCFVLLIPLAATSTNNMVKRLGAKRWQQLHRFVYFIAAGGVVHFWWLVKKDISEP
                     LQYAVLLSLLLALRALFWLQKNGLPKFAKLTS"
     gene            complement(5286..6245)
                     /gene="msrP"
                     /locus_tag="VLL03_05280"
     CDS             complement(5286..6245)
                     /gene="msrP"
                     /locus_tag="VLL03_05280"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HSS46809.1"
                     /translation="MLIKKPADIWPSEITPQDLYVRRREFIKAVALTAAGAAMPALFN
                     TPREARGATPATPFTHLRSSTYSTLEEKTPFKDVTHYNNFYEFGTDKYSPAERAGSLK
                     TRPWVVAVEGEIRRPKVYDADELLKLAPLEERVYRLRCVEGWSMVIPWAGYPLSELIK
                     RVEPTDKAKFVEFITLYDTGQMPGQRVPVLSWPYVEGLRLDEAMHPLTLLCFGLYGQA
                     LPNQNGAPMRIVVPWKYGFKGAKSIVRIRFVEKQPVSAWTKAASHEYGFYSNVNPNVD
                     HPRWSQAKERRIGEFFKRPTLMFNGYADQVASLYTGMDLRKNF"
     gene            complement(6382..7290)
                     /locus_tag="VLL03_05285"
     CDS             complement(6382..7290)
                     /locus_tag="VLL03_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010110366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSS46810.1"
                     /translation="MRWPWKSFRRIATAAVLTLLAGCSPFAILNATAPQNHYAVKRDI
                     AYGELERQRLDVYTPRSQSGQAPVVVFLYGGSWESGERGEYAFIAEALTSLGFVAVIP
                     DYRVYPEVKFPTFLEDCARAVKWAKDHIGEHGGDAKLIYVMGHSAGAYNAAMLAFDKQ
                     YLEAVGMSPKMLRGFIGLAGPYDFLPLKKPALIDIFGGKDNIPHTQPINFVTRDAPPA
                     LLLVGNTDTTVKPGNTTRLAARIREKSGEVREVVYDGIGHFRILGALSAPFRGWAPVL
                     RDVAQFINSTSVQPVTGKPAAASQVN"
     gene            complement(7358..>8487)
                     /gene="mgtE"
                     /locus_tag="VLL03_05290"
     CDS             complement(7358..>8487)
                     /gene="mgtE"
                     /locus_tag="VLL03_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HSS46811.1"
                     /translation="ETLIASMEPAELVAAAETLDTDEIAELAPDLPRDVVQDIIDSLD
                     AQNRARLQTKLAYPEDTVGALMDYDMVIIREDIGVEVVLRYLRRFDEIPGQTDALFVV
                     NRDDVLRGVLPLKRLLVSDPEVQVSALMLQDPVSFHAEDKANQAALAFERYGLVSAPV
                     VNGNHKLIGRITVDAVVEFIREKSDTQLLSSAGLREEEDLFAPVKESTKNRTLWLGIN
                     LITAFAASAVTHLFEASIEKIVALAVLMTVIPSMGGNAGTQTMALVTRGFALDQINRD
                     NIRHLFNKELAVAYYNGLIWAAVVGALAALWYHDFKLSLVFGAAMIVELFAAAVSGVT
                     IPLILRRFKIDPAVGTTVILTTVTDVVGLFSFLGLATLFLV"
BASE COUNT         1886 a         2499 c         2395 g         1707 t
ORIGIN      
        1 tcttgagcct cccacaggaa tgcctaattc tttcgatgaa gcagcatcag agatacttcc
       61 cgctcgccaa taaggtgggc aaactgttgc cgtgcttttt gatcgtgagc aacctgcaga
      121 ctaccagccc caaaaatatc attcacggca atgagcgggt gctgcgtgcg cgcctttcgg
      181 atgccaagtt tttttacgag cacgaccaga aaaccaagct agtggatcgt gtgccaaagc
      241 ttgtcgacat cgtctatcac aacaagctgg acaaccaatt ggcgcgggta aaacgtttgc
      301 aaaaactcgc aagcagtatc gcccgagaaa tcagaacaga tgtggcgcat gctggacgcg
      361 ccgcttatct gtgcaaggcc gatttactca ctgacatggt gggcgagttt cccgaactgc
      421 agggcatcat gggccgttac tatgcattgc acgacggcga gcctgaagag gttgccgatg
      481 cgattggagc gcattatcgt ccgcgctttg cgggcgatac aacacccgat ggacgcatcg
      541 cctctgcagt agccctcgca gacaaactcg acacactagc cggtctcttt ggcatcggtc
      601 aggttccgac gggagacaaa gatccgtttg ggttgcgtcg agcggctctt ggagtcatac
      661 ggattctgat tgaacgagat cttccgcttt ctttgcatga gttagtcaac gcggcttttg
      721 ccggctacaa cgggcgtgtt ggagatgctc acactgatct cgaatcattc atcttcgacc
      781 gccttgccgg atatttgagg gaccgtgggt attcgacgct ggaagtcgag tcagtcgtaa
      841 gtctccggcc cgaccagatt aatttggtcc ccagccaagt tgaggccgtg cgagcgttta
      901 atgcgctgcc cgaagcgcaa agtcttgccg cggccaacaa gcgcgtggta aatattctca
      961 aacaggccga ggctaatgga gaatcctttg ttaatgccga agctgataag ctacaagagc
     1021 ccgcggagcg agcactattc gatgcgctca aaaccacgtc gcggcaagct gcttcacttt
     1081 tcaaccaagg taactttacg ggctatttga aaactttcgc gattctcaaa tccccagtgg
     1141 acgctttttt tgactccgtc atggtaatgg cggatgcacc cgagctgcgg cacaatcgcc
     1201 ttgcgctcct cgccgatctt cgcaaggaaa tgaaccgggt ggctgatatc tcgaagctgg
     1261 ctgtctgaca tgaaactcgt cattctcgac cgcgacggcg tcatcaacca cgatagcgcg
     1321 caatacatta agagcccgga cgaatggaag ccgattgccg ggagcctcga ggccatcgcg
     1381 cgcctgaatc aggccggcta ccgcgtggtg gtggtgacca accagtcggg aatcgggcgc
     1441 gggctgttcg acatgacggc gctaaacgcc atccatgaca agatgcataa gtcactcacg
     1501 cagtatggcg gccggattga cgcgattttc ttctgcccgc acgcacagga actgagttgc
     1561 gcctgccgca agcccaagac cggcttgttc caggaaatcg cgcagcgctt taacgtcaat
     1621 ctcgccggcg tgccctgcat aggcgattcg gcgcgcgact tgcaggcggc gcacgccgcc
     1681 ggtgcgcaac cgatactggt gctcaccggc aagggtaaaa aaaccaggga agccggaaat
     1741 ctgcctgaaa gcactcagat atacgccgac ctcgcggaag cggcgcaggc gcttgccgct
     1801 tagccgatgg ttgtcctgcg ttccctgttt tttgcgctgc tccaagccgt cgtcactccc
     1861 ctgtttgctt taatcgctct tcttaccttt cccttcgacc cttttacccg ctaccacatc
     1921 attaccacct ggtcgcgcat catcatcggc acggcgcgcg cgatctgcgg catccgctat
     1981 cgcatcgtag gcagggaaaa cattcctgac catccctgca ttatcatgtc caagcaccaa
     2041 tcagcatggg aaacactggc gttccaggta attttcccgc cgcaggcact ggtgattaaa
     2101 agagagttgt tgcgcatccc gttcttcggc tgggggctcg ccatgctttc tcccatcgcg
     2161 attgaccgcg acgcgggaac ccgggcgctc aggcaaatcg aggagcaagg catcgcacgg
     2221 ctgcaacagg gtttctgggt gattgtcttt cccgaaggca cgcgcatcaa acctggagaa
     2281 aagggtgtat accaaccggg tggggcctgg cttgcgaccc actctaacag catggtcctg
     2341 ccgatagcgc tcaattccgg cgaattttgg cgccgtaatg ctttcctgaa gcgtccgggt
     2401 accatcacgg tgagcatcgg cccgccgatt cgggcgcaag ggatgaaacc cggcgaattg
     2461 aacgccaaag tggaaacctg gatcgaatcg gagatggcgc gcatcgccaa gcatgaagtc
     2521 tgagcggccc gctgcctttg ccgcgccgga ccgtatgtta agattcaact tttgctataa
     2581 cccaaacccg ccatgcgtgc attcgttttg ctgatcgtgc tcgccgtgct gatgcaaggc
     2641 tgtggttaca aaggcccact ttatctgccg ccacaacagc cggcccagca gcccaccccg
     2701 ccgccgcaaa agcaggacgc gaacaagcaa cagggttcgc cgaacccgtg acgtactttt
     2761 cctatcgtaa taaacagctg catgccgaag cagtggcgct tgcccatgtc gccgcccaat
     2821 tcggcacgcc ctgttatatc tattccaaag cggcgctcac cgcagcgtac caaagtttcg
     2881 cccaggcgct cgccaaacgc gagcatctga tttgctacgc ggtcaaggca aactccaacc
     2941 tcgctatcct gaacctcttc gcacggctgg gtagcggctt cgatatcgta tccggcgggg
     3001 aactggcgcg cgtgctcgcc gccagcggcg atccgcgcaa agtggtattt tccggcgtgg
     3061 gcaaaagcgc cgaggaaatg cgcgcggcgc tgaacgccgg catcctctgc ttcaatgtcg
     3121 aatcggaagc ggagctggtg cggcttgacc gcgtggccgg tgaaacgggg aaaaaagcgc
     3181 cggtgagcct gcgcatcaat ccggatgtga accccaaaac ccatccctac atcgccaccg
     3241 gcttgaaaca gaacaaattc ggagtggatt acgcggacgc cctggcgctc taccgcaaag
     3301 caggctcgat gtcccacctc caaatcaccg gcattgactg ccatatcggc tcgcagctta
     3361 ccgaggtcgc tccctttacc gacgccctgg aaaagatcct gctgcttatc gacaagctgg
     3421 aaaaaaccgg cgtccgtatt gcccacatcg acatcggcgg agggctgggc atccgctatc
     3481 gcgacgaaac tccgcccaat ggcgccgagt acgccgccgc gctgtttgcc aagctcggcc
     3541 ggcgcacgca gacgattctg ctcgagcctg gacgcgcgct ggtcggcaat gccgggttgc
     3601 tgctcacccg cgtcgagtat ctcaagcata accgcgacca caatttcgcc atcgtggacg
     3661 ccgctatgaa cgacctggtg cgtccggcgc tctacgatgc ctaccacgac attctgccgg
     3721 tcatgcagtc gcagcgctca aaacgcacat acgaaatcgc cgggccggtg tgcgaaagcg
     3781 gcgatttcct cggccgcggc cgcaagctgg cactcgcgga aggcgatttg ctggcggtga
     3841 tgtccgccgg cgcctacggc atgagcatga gttcgaacta caacacccgc ccgcgcgcgg
     3901 cggaagtaat ggtggatggc gatcaagcga ttctcatccg cggccgcgag agcgcgaatc
     3961 agcttttcgc aggggaaaaa cttcttcctt agaaccgcgt caacggcacg taaaaacatc
     4021 ggacccgcgc ctgtggaatg gtttttttgc ggttcaactc gccgccagcc gatttctccc
     4081 gccgaaaaaa atttgcaaaa aaatgaaaaa atatgttgtg ttttccaaaa taattggaat
     4141 agaattggcg caccaagcat cagttatcgt tctttcaagc aagtttggga gaaacatcgg
     4201 agcaaatcgc ttgatgccga tgcttcggga gtgcggcgca ataccccgca ctaagtaacg
     4261 agaaactttt taaaaggagt caaaatggca gccaaaaaga agtcatccaa gaaaaaaggt
     4321 aagaagaaga agtaaataaa aatataaggc tggtttcttg taaaccagcc tttttacttt
     4381 taccaggcgg ttattttgac cgcctgagtt ttttctggct caataaaatc cctcccccac
     4441 cccggtttaa gcccgcagcc gtcacatgaa atccagccgc aaaaccccgc tcaagcgccg
     4501 caaccgcgtg gcgctaagcc cgctgttgcg caaaggcggg gcgcacgcgc tccaaaacaa
     4561 gaaagcgaaa cgggcgctac agaaggcaag gcttcggaaa accctggcgg aagagcttta
     4621 ggaggtcagc ttggcaaatt tgggcaggcc gttcttttgc agccagaaca gcgcccgcaa
     4681 ggcaagcagc aaagagagca gaaccgcata ctgcaagggt tccgagatgt cctttttcac
     4741 cagccaccag aagtgcacta ccccgccagc agcgatgaaa tagacgaaac ggtgcagttg
     4801 ctgccagcgc ttcgcgccca gtcgcttcac catgttgttg gtggaagtcg cggcaagcgg
     4861 gatcagcagc acaaagcagg tgaagccgag ggtgatgaaa gggcgcttca acacgtcttt
     4921 gccgatttcg aagatgtcga aaaactggtc gagccagatg taggtgagga aatgcagaca
     4981 ggcgtagaaa aaggcgtaca acccgaacat gcggcgcaat ttaatcagcc acggccactc
     5041 aagcaggcgc cgcagcggcg tgactgacaa agttataagc aggaatatca gcgtccagtc
     5101 gccggtggta tgggtcacca cctcgatagg attggcgccc agatcatgat tgaagccgcg
     5161 ccaggcaagg cgcgccagcg ggaccaggca ggccacgaac agtccggcct tgagcctttg
     5221 aaaacgttgt ggtggaagtt gggcaaaaga catgaacggg gaaaacgcga agtcgcgtcg
     5281 cggcactaga aattcttgcg caaatccatg ccggtgtaga gcgaggcgac ctggtcggca
     5341 taaccgttga acatcaaggt cgggcgcttg aagaactcgc cgatgcggcg ttccttggcc
     5401 tggctccagc gcgggtgatc cacattcgga ttcacattgg agtagaagcc gtattcgtgc
     5461 gatgccgcct tggtccaggc gctcaccggc tgtttttcga caaagcggat gcgcacgatg
     5521 gatttggcgc ccttgaagcc gtatttccac ggcaccacga tgcgcatcgg cgcaccgttc
     5581 tggttgggca gcgcctggcc atacagccca aagcaaagca gggtaagcgg gtgcatcgcc
     5641 tcatccaggc gcaaaccctc gacataaggc caactcaata ccggcacgcg ctggcctggc
     5701 atttggccgg tatcataaag tgtgatgaat tcaacaaatt tcgccttgtc tgtaggttcc
     5761 acgcgcttga tcagttcgga aagcgggtaa cctgcccacg gaatcaccat tgaccagcct
     5821 tccacgcagc gcaggcggta aacgcgctct tccagcggtg cgagcttgag cagttcatcg
     5881 gcgtcataaa ccttcggccg cctgatttcg ccctccaccg ctaccaccca gggacgggtc
     5941 ttcaggcttc cggcccgttc cgcgggggaa tatttgtccg tgccgaactc ataaaaattg
     6001 ttgtagtggg taacgtcctt aaacggcgtc ttctcttcca gcgtgctgta ggtgctgctg
     6061 cgcagatggg taaacggcgt cgccggcgtt gccccacggg cttcgcgcgg cgtattgaaa
     6121 agggccggca tggcggcgcc tgcagccgtc aaagccactg ccttgatgaa ctcgcggcgc
     6181 ctgacgtaga ggtcctgcgg cgtgatttca gaaggccaga tatcggcggg tttcttgatc
     6241 agcattacca gctcctaaat tcgggggatg gacatgggtc gcggcgcgcg tccatttctt
     6301 acattttacg cgtattccgg ctttgacagt ttcgacttcg gctggcggct ggagttcgcc
     6361 cctgtccttc gcccagcggg gctagttgac ttggctggca gctgcaggct tgcccgtcac
     6421 tggctggacc gaagtgctgt taatgaattg cgcgacatca cgcagcaccg gcgcccagcc
     6481 gcggaatggc gcagacagcg cgcccaagat gcgaaagtgg ccgatgccgt cgtaaacgac
     6541 ttccctgacc tcgccgcttt tctcgcggat gcgtgcggca agccgcgtgg tattacccgg
     6601 tttgaccgtg gtatcggtgt tgcccaccag gagcagtgcc ggcggtgcgt ctctggtcac
     6661 gaaattgatc ggctgggtat gaggaatatt atccttgccg ccgaaaatat caatgagcgc
     6721 cggctttttg agcggcagga aatcataggg accggcaaga ccgatgaaac cgcgcaacat
     6781 ttttggactc atgcccaccg cttccaggta ttgtttgtca aacgcgagca ttgccgcatt
     6841 gtatgcgccg gccgaatgtc ccatcacata aatcagcttc gcatcgccgc catgttcgcc
     6901 aatatgatcc ttagcccatt tcactgcacg cgcgcagtct tcgagaaacg tcgggaattt
     6961 cacttcggga taaacgcgat aatccggaat cacggcgaca aagccgagtg aagtcaacgc
     7021 ctcggcgata aatgcgtatt ccccgcgctc gccggattcc caggaaccgc catacaaaaa
     7081 caccaccact ggagcctgcc cgctctggct gcgcggagtg taaacatcga ggcgttggcg
     7141 ttccaattcg ccgtaagcga tatcgcgctt gaccgcataa tggttttgcg gagccgtcgc
     7201 attgagaatg gcaaaaggcg aacagccggc gagcagcgtg agtacggcgg cggttgcgat
     7261 acgtctgaac gatttccagg gccatcgcat gggcatttcc tcccgttcct tgagcaccag
     7321 tttatatacg ggtcagcgcc aggatgctct ggccagctca aaccaggaat aaggtggcaa
     7381 gccccaggaa gctgaacaaa cccaccacgt cggtcacggt ggtgaggatc actgtggtgc
     7441 ccaccgccgg atcgatcttg aaacggcgca gaatgagcgg gatcgtcacg ccggacaccg
     7501 cggcggcgaa aagctcgacg atcatcgccg cgccgaacac caggctcagt ttaaaatcgt
     7561 ggtaccacag cgccgctaat gcccccacca ccgctgccca gataaggccg ttgtaatagg
     7621 ccaccgccag ttccttattg aacagatgcc ggatgttgtc gcggttgatc tggtcaagcg
     7681 caaagccgcg ggtgaccagg gccatggtct gcgtgccggc gttgccgccc atgctgggaa
     7741 tcaccgtcat cagcacggcg agcgccacga ttttctcaat cgaggcctcg aacaaatggg
     7801 tgaccgccga agcggcaaaa gcagtgatca ggttgatgcc cagccacagg gtgcggttct
     7861 tggtgctctc cttgaccggc gcgaacaagt cttcctcctc gcgcaggccg gcgctgctca
     7921 gcaattgggt gtccgatttc tcgcggatga attccaccac cgcatcaacc gtaatccggc
     7981 cgatgagctt gtggttgccg ttcaccaccg gcgcggatac caggccataa cgctcgaaag
     8041 ccagcgccgc ctgattggct ttgtcctcag cgtgaaagct tacgggatcc tgcagcatga
     8101 gcgcgctcac ctggacttcc ggatcgctca ccaaaaggcg cttgagaggc aacaccccgc
     8161 gcaacacatc atctcggttc accacgaaca gcgcgtcggt ctggccggga atttcgtcga
     8221 aacggcgcaa atagcgcagc accacctcga ctccgatgtc ctcgcggatg atcaccatgt
     8281 cgtaatccat gagcgcgcct acggtgtctt ccgggtaggc gagcttggtt tgcaggcgtg
     8341 cgcggttctg cgcatccagc gaatcgatga tgtcctgcac cacgtcgcgc ggcaggtcgg
     8401 gcgccagctc ggcgatttcg tcggtgtcca gcgtttcggc ggcggcgacc agctccgccg
     8461 gctccatgct ggcaatcagc gtctcgc
//
LOCUS       DATIOW010000158         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_374685, whole genome shotgun sequence.
ACCESSION   DATIOW010000158 DATIOW010000000
VERSION     DATIOW010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374685"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1760)
                     /gene="uvrA"
                     /locus_tag="VLL03_05295"
     CDS             complement(<1..1760)
                     /gene="uvrA"
                     /locus_tag="VLL03_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HSS46812.1"
                     /translation="MDFIKIRGARTHNLKNINLDLPRNRLIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLQLMEKPDVDLIEGLSPAISIEQKAASHNPRSTVGTVT
                     EIHDYLRLLFARVGDPYCPDHQIPLQAQSVSQMVDHVLQLPLDTKLMILSPLIVGRKG
                     EQMELFDELRAQGFVRLRINGKVYEIDNLPKLQKTRKHTIEVVVDRLKVRPDVKQRLA
                     ESFETALRHADGRALAVEIESGREHLFSAKFACPVCSYSLPELEPRLFSFNNPMGACP
                     RCDGLGQISFFDPKRVVAFPHLSLASGAIKGWDRRNQFYFQMLASLASHYQFDLEDPF
                     EKLPIPVQEVVLNGSGREKIKFTYLGERGNNYQREHAFEGIIPNLERRYRETDSLVVR
                     EELAKYLNSQPCPLCQGTRLRREARYVRVANQTIYEISAMPLQQTVRFFENMNLSGAK
                     RAIAEKIVKEIISRLQFLNNVGLDYLSLGRSADTLSGGEGQRIRLASQIGSGLTGVMY
                     VLDEPSIGLHQRDNGRLLDTLKRLRDLGNSVIVVEHDHEAIMSADHVVDMGLGAGEHG
                     GRVVAEGTPLAIQKHPESLTG"
     gene            1826..>2066
                     /locus_tag="VLL03_05300"
     CDS             1826..>2066
                     /locus_tag="VLL03_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSS46813.1"
                     /translation="MTFSDRMSPLELRASLGLASIFGLRMLGMFVILPVFALYAEDLP
                     GGVSHTLVGIALGAYGLTQAILQIPFGWLSDRIGRK"
BASE COUNT          390 a          608 c          617 g          451 t
ORIGIN      
        1 ccggtcagcg attccggatg tttctgaatc gcaagcggcg tgccttccgc aaccacccgg
       61 ccgccgtgct cgccagcgcc caaacccatg tccaccacgt gatctgcgct catgatcgct
      121 tcgtgatcgt gctccaccac gatgacgctg ttgcctagat cgcgcagccg cttcagggta
      181 tccagcaggc gcccgttgtc gcgttgatgc aggccgatgg aaggctcgtc cagcacgtac
      241 atcacgccgg tcaatcccga accgatctgg ctcgccaaac ggatgcgctg tccctccccg
      301 cctgagagcg tatcggcgga acgaccgagc gagagataat cgaggccgac gttgttcaga
      361 aactgcaggc ggctgataat ttccttgacg attttttcag cgattgcccg cttggcgccg
      421 cttaagttca tgttctcgaa aaaccgcacg gtttgctgta gcggcatggc gctgatttca
      481 taaatcgtct ggttcgccac gcgcacgtac cgcgcttcgc ggcgcagccg cgtgccctgg
      541 cacaacgggc agggctggct gttcagatat ttcgccagtt cttcgcgcac caccagcgaa
      601 tcggtctcgc ggtagcgccg ctcgaggttg ggaatgatgc cctcgaaggc gtgctcgcgc
      661 tggtagttgt tgccgcgctc gccgagatag gtgaatttga ttttctccct gccgctgccg
      721 ttcaagacca cttcctggac gggtatcggc agcttttcaa aagggtcttc caggtcgaat
      781 tgataatgag aggccaagct tgcgagcatc tggaaataaa actggttgcg ccggtcccag
      841 cctttgatgg cgcctgaagc gaggctcaaa tgcgggaaag cgaccacgcg cttggggtcg
      901 aagaagctga tttgtcccaa gccgtcgcaa cgcgggcagg cgcccatcgg gttgttgaag
      961 gaaaacagcc tcggctcgag ctccggcaag gaataactgc acaccggaca ggcgaatttg
     1021 gcggagaaca gatgctcgcg cccgctttcg atttccaccg ccagcgcgcg cccgtcggca
     1081 tggcgcaacg cggtctcgaa cgattccgcg aggcgctgct tgacatcggg acgcactttg
     1141 aggcggtcca ccaccacttc gatggtgtgc ttgcgcgttt tttgcagctt gggaagatta
     1201 tctatttcgt acactttgcc gttgatcctg agccgcacga aaccctgcgc gcgcaattcg
     1261 tcgaagagtt ccatctgctc ccctttgcgc cccacaatca gcggcgagag gatcatcagc
     1321 ttggtatcca gcggcagttg cagcacatgg tccaccattt gcgagacgct ttgcgcctgc
     1381 agcgggattt gatggtcggg gcagtaaggg tcgccgacgc gcgcgaacaa aagccgcagg
     1441 taatcgtgga tttcggtgac ggtgccgacg gtggaacgcg ggttgtgcga ggccgctttt
     1501 tgctcgatgg aaatcgccgg agagaggccc tcgatcaagt ccacgtcggg tttttccata
     1561 agctgcagaa actgacgggc gtaagccgag agcgattcca catagcggcg ctggccttcg
     1621 gcgtacagcg tgtcgaacgc cagcgaggac ttgcccgagc cggataagcc ggtaatcacg
     1681 atgagcctgt tgcgcggcaa atcgagattg atgtttttca gattgtgcgt gcgtgcgccg
     1741 cggattttga tgaagtccat gaactgttcc ggcaacggtc aacctgttaa tataccgcag
     1801 ttcgcaagtt tttcccaacc tctctatgac tttctccgac agaatgagcc cgctggaact
     1861 gcgagccagc ctgggtctcg cctcgatttt cggcctgcgc atgctgggga tgttcgtgat
     1921 tcttccggtg tttgcgctct atgccgagga cttgccgggc ggcgtcagcc ataccctggt
     1981 gggaattgcg cttggcgcat acgggctcac tcaggcgatt ctgcaaatcc cattcggctg
     2041 gctctccgac cgcatcgggc gcaagc
//
LOCUS       DATIOW010000159         3142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1999801, whole genome shotgun sequence.
ACCESSION   DATIOW010000159 DATIOW010000000
VERSION     DATIOW010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1999801"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..597
                     /locus_tag="VLL03_05305"
     CDS             127..597
                     /locus_tag="VLL03_05305"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HSS46814.1"
                     /translation="MNVTATLFGQMITFAVLIWFIHRFLWGPLTRMLAERSKRIADGL
                     AAAERGKRELEIAGKRSAETLRDGKRQSADIIAQAEKRALQIIEEAKHAAKAEGDRMI
                     AGAKAEIAQEVSRAKEALRSQVSALAVAGAEKILRREVDAKVHAELLKGIQTEL"
     gene            606..1142
                     /locus_tag="VLL03_05310"
     CDS             606..1142
                     /locus_tag="VLL03_05310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HSS46815.1"
                     /translation="MAENVTIARPYAEAVFKLAKEKGTLSQWAKMLALLEAVVKDPHV
                     EAYIGNPNFSAGQIESLLLGICGKKLDGMGRNFVQVLAHNDRLILLPEIRELFEALKA
                     EQEGVLEVTINSAFPLDKKQAGELVRRLKDRYQRKIVTQVSVEPELIGGLKIAVGDHV
                     LDATVRGKLETMRAALTR"
     gene            1174..2715
                     /gene="atpA"
                     /locus_tag="VLL03_05315"
     CDS             1174..2715
                     /gene="atpA"
                     /locus_tag="VLL03_05315"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HSS46816.1"
                     /translation="MQVNPSEISELIKSRIEKLAVTAESRTQGTVISVTDGICRVHGL
                     SDVMQGEMLEFPKNTFGLALNLERDSVGVVVLGEYEHITEGDTVKCTGRILEVPVGEA
                     LIGRVVNSLGQPIDGKGPIQAKETSPIEKIAPGVVWRKSVSQPVQTGLKSIDSMVPIG
                     RGQRELIIGDRQTGKTAVAVDTIINQQGKDLICIYVAIGQKASSIVNVVRKLEEHGAM
                     AYTIVVAASASESAAIQYIAPYSGCSMGEYFRDRGQDALIIYDDLTKQAWAYRQVSLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEEYVERYTHGKVKGKTGTLTALPIIETQA
                     GDVTAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     VRLSLAQYRELAAFAQFASDLDEATRKQLERGRMVTELMKQPQYEPLQVWEMALTLFA
                     VNNGYFDDIDVKKALSAERAMRDYIKGKYADLVKRIEDTKDLSKDDESKLHEAMKDFK
                     KNAAY"
     gene            2766..>3142
                     /locus_tag="VLL03_05320"
     CDS             2766..>3142
                     /locus_tag="VLL03_05320"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HSS46817.1"
                     /translation="MPGSKEIRNKIKSVQNTRKITKAMEMVAASKMRKAQERMRRARP
                     YGEKIRNIAAHLSHANPEYRHPFLVERDTVKKVGLVLITTDKGLCGGLNTNVLRLTLS
                     KMKEWEAQGEQFEVCCIGNKGLG"
BASE COUNT          749 a          854 c          974 g          565 t
ORIGIN      
        1 gccatgtatt tcacgttcgc taacccgttc ttaggggccg cgctgaacgc gctcaagacg
       61 gcaggcggct aacatgactg gggttccacg agtagcagcg tcgcaaacga aaaaaggagt
      121 ttggacgtga acgtgacggc cacacttttt ggtcaaatga ttacctttgc ggtgttgatt
      181 tggtttattc accgctttct ttggggtcct ttgacccgca tgctggcgga gcgcagcaag
      241 cgcatcgctg acggccttgc cgccgcggaa cgcggcaagc gggagctgga aattgccggc
      301 aagcgctcgg cggaaacgtt gcgtgacggc aaacggcagt ctgcggacat catcgctcag
      361 gcagagaaac gcgccctgca aatcatcgag gaagccaagc acgcagccaa ggccgagggc
      421 gaccgcatga tcgccggcgc caaggcggaa atcgcccagg aagtgagccg cgccaaggaa
      481 gcgctgcgca gccaggtatc ggctttggcg gtggcgggcg ccgagaaaat tctgcgccgc
      541 gaagtagacg ccaaggtgca cgccgagctg ctgaaaggca tccaaaccga actctaggaa
      601 atagcatggc agaaaacgtt actatcgcgc ggccgtacgc ggaagccgta ttcaagctgg
      661 ccaaggaaaa aggcaccctg tcccaatggg cgaagatgct ggcgcttctg gaggcggtgg
      721 taaaagaccc gcatgtggag gcttacatcg gcaacccgaa tttcagcgct gggcaaatcg
      781 agagcctgct gctcggcatc tgcgggaaga aactcgacgg catgggcagg aatttcgtgc
      841 aggttctcgc gcacaacgac cgccttatct tgctgccgga aatccgcgag ttgtttgaag
      901 cgctcaaggc ggaacaggaa ggcgtgctcg aagtgacgat taattcggcg tttccgctgg
      961 acaagaaaca ggcgggagaa ctggtgcggc gtctcaaaga caggtaccag cgcaaaatcg
     1021 tcacgcaggt gagcgtggaa cccgagctga taggcggcct taaaatcgcg gtcggcgacc
     1081 atgtactcga tgccacggtg cgcggcaaac tggaaacgat gcgtgcagca ttgacaaggt
     1141 aaagcaaacc ccaggttttg cttaggagca gaaatgcaag ttaatccctc ggaaatcagt
     1201 gaactgataa aaagcaggat cgagaagctc gcggtgacgg cggaatcgcg cactcagggc
     1261 acggtgattt ccgtaaccga cggcatctgc cgggtgcacg gcctttccga cgtgatgcag
     1321 ggcgagatgc tggagtttcc gaaaaacact ttcggcctcg cgcttaactt ggagcgcgac
     1381 tcggtgggcg tggtggtgct gggtgaatac gagcacatca cggaaggcga taccgtgaaa
     1441 tgcaccggcc gcattctgga agtcccggtg ggcgaggcgc tgatcgggcg cgtggtgaat
     1501 tcgctcggcc agccgataga cggcaaaggt ccgattcagg ccaaggaaac ttcgcccatc
     1561 gaaaaaatcg cgccgggagt agtgtggcgc aaatcggtgt cgcagccggt gcagaccggc
     1621 ttgaagtcca ttgactccat ggtgccgatc ggccgcggcc agcgcgaact catcatcggc
     1681 gaccgccaga cgggcaagac ggcggtggcg gtggacacca tcatcaacca gcagggcaag
     1741 gacctgattt gcatttatgt cgcgatagga caaaaggcat ccagcatcgt caacgtggtg
     1801 aggaagctcg aagagcacgg cgccatggcg tataccatcg tggtggcggc ttccgcctcc
     1861 gaatccgcgg cgatacagta tatcgcaccc tattccggct gcagcatggg cgagtacttc
     1921 cgcgaccgcg gccaggacgc gctgatcatt tacgatgacc tcaccaagca ggcctgggct
     1981 tatcggcagg tttcgctgtt gctgcgccgc cctccggggc gcgaagcgta tcccggcgac
     2041 gtgttctatc tgcactcgcg tctgttggag cgtgcagcgc gagtgaacga ggaatatgtc
     2101 gagcgctaca cccatggcaa ggtcaagggc aaaaccggca ccctcaccgc gctgccgata
     2161 attgaaaccc aggccggcga cgtgaccgcg ttcgtaccga ccaacgtgat ttcgattacc
     2221 gacggccaga tttttttgga aaccgacctg ttcaacgccg gcattcgtcc cgccatcaac
     2281 gccggcatct cggtgtcgcg ggtgggaggt gcggcgcaga ccaaggtgat taaaaagctg
     2341 ggtggcggag tgcgtctttc cttggcgcaa taccgcgaac tggcggcttt tgcgcagttt
     2401 gcttccgatc tcgatgaagc gacgcgcaag cagttggaac gcggccgcat ggtaaccgag
     2461 ctcatgaaac agccgcagta cgagccgcta caggtgtggg aaatggcctt gacgctgttt
     2521 gccgtgaaca acggttattt cgacgatatt gatgtgaaga aggcgctttc cgccgaacgc
     2581 gcgatgcgcg attacatcaa gggcaagtac gcagacctgg tgaagcgcat cgaggacacc
     2641 aaggatttgt ccaaagacga cgaaagcaag ttgcatgaag caatgaagga ctttaagaag
     2701 aacgccgcgt actaatggca agctgctgcg ctcgctacgt ttgctgacgt atagagagtt
     2761 tataaatgcc aggttcaaaa gaaattcgaa ataagattaa gagcgtccaa aacacgcgca
     2821 aaataaccaa ggccatggaa atggtcgcgg cctcgaaaat gcgcaaggcg caggagcgca
     2881 tgcgccgcgc ccgtccttat ggcgaaaaaa tccgcaatat cgctgcgcat ctctcccacg
     2941 ccaacccgga ataccgtcac cccttcctgg ttgagcgcga cacggtgaag aaagtcggcc
     3001 tcgttctgat taccaccgac aagggcctgt gcggggggct caacaccaac gtgctcaggc
     3061 tcaccctcag caaaatgaaa gaatgggaag ctcagggcga gcagttcgag gtctgctgca
     3121 tcggcaacaa aggccttggc tt
//
LOCUS       DATIOW010000160         2951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_578642, whole genome shotgun sequence.
ACCESSION   DATIOW010000160 DATIOW010000000
VERSION     DATIOW010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578642"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VLL03_05325"
     CDS             <1..878
                     /locus_tag="VLL03_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011514944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSS46818.1"
                     /translation="AGLEVPTAGDILIGGKIVTLFPPKARNIAMVFQSYALYPHLSVF
                     KNIAFPLEAQGMKSEAVKSKVEHAAAMFGIGRLLARKPRQLSGGERQRVALARAVVRE
                     PVVFLLDEPLSNLDAKLRTTARDELQQFQRRLGTTTVYVTHDQIEAMGLGDRIAVLNH
                     GKLHQLGTPQEVYVDPADTFVATFIGSPPMNLVEEAPHTIGFRPEQFLPIECYNDGDD
                     LIRFAFTVTRLEYLGADRLVYGTLMAHPEVKVISKLPTSVTVAVEKGQCHEFAVPRKD
                     LKRFDPQSGLKVTAE"
     gene            888..1808
                     /locus_tag="VLL03_05330"
     CDS             888..1808
                     /locus_tag="VLL03_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HSS46819.1"
                     /translation="MSNPTAALQARSPLLEEEKWLGRLMLAPAVIYIVALVGFPFLLA
                     IYYSLSDATVGSQTLHFVGFRNFQRILENSTFWTSLRNALVFTFVSQLLVVILGKALA
                     MALYKDFRGKWLVRFLILLPWVAPISLGSIGWLWIFDPIYSIINWTARAIGLFGPHTW
                     PIWLGQPNLAMASVIMVDVWRLLPLATVIILAGLSSIPQDIHDAAEVDGASFWRHLTY
                     INIPLLMPIMLVALLFGIVFTFTDMIVIYVLTRGGPYDTTQVIASLAYFTGIQGGDLA
                     EGAAISLFLFPLLVAAAILLLTFAKRTEVT"
     gene            1809..2651
                     /locus_tag="VLL03_05335"
     CDS             1809..2651
                     /locus_tag="VLL03_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HSS46820.1"
                     /translation="MQRLKNIGGRIGHFGIIAAFTAFSAFPFYWMLITTFKQTNDLIN
                     VKNNPFIYNSPPTLEHLRILLVDTQYTQWLLNTLGVGVLVVLITLLLAVPAGYSLARL
                     AGRWGQQLAIGIFLTYLVPPTILFIPFSRIVADLGLQDSLWSLVLVYPSFTVPFCTWL
                     MMGFFKSVPKDIEEAAMIDGLSRFGAFLKVVVPISSAGILTVIIFTLTLVMQEFVYAL
                     TFITSSSQLTVSIGVPTFLVRGDVYFWGSLMGACLIVSIPIAILYNFFVDRFVAGFTV
                     GAVK"
     gene            2759..>2951
                     /locus_tag="VLL03_05340"
     CDS             2759..>2951
                     /locus_tag="VLL03_05340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HSS46821.1"
                     /translation="MAEKHHFNPRQLRRIFSQVKPQPEVLKAITAPPTALPWNEYRAL
                     FINPARITDGVKFWDEHETA"
BASE COUNT          538 a          879 c          847 g          687 t
ORIGIN      
        1 ttgccggcct ggaagtgccg accgccggcg atattctgat cggcggcaag attgtcaccc
       61 tgtttccgcc caaggcgcgc aacatcgcca tggtgtttca gagctacgcg ctttatcccc
      121 atctcagcgt gttcaagaac atcgcttttc ctttggaagc gcagggcatg aaaagcgagg
      181 cggtgaaaag taaagtcgag cacgccgcgg caatgttcgg catcggccgc ctgcttgcgc
      241 gtaagccgcg ccagctgtcc ggcggcgagc ggcagcgcgt ggcgctcgcc cgcgccgtgg
      301 tgcgcgagcc ggtggtgttc ctgctcgatg agccgctttc caatctcgac gccaagctgc
      361 gcaccactgc gcgcgatgag ctgcagcaat tccagcgccg gctcggcacc accaccgttt
      421 acgtcaccca cgaccagatc gaagccatgg ggttgggcga ccgcatcgcg gtgctgaatc
      481 acggcaagct gcatcagctc ggcacgccgc aggaagtcta tgttgacccc gccgacacct
      541 ttgtcgccac cttcatcggt tcgccgccga tgaatttggt ggaagaagcg ccgcacacca
      601 tcggtttccg tcccgagcag ttcctgccga tcgagtgcta caacgacggc gatgatttaa
      661 tccgctttgc ttttacggtg acgcggttgg aatatctggg tgccgaccgc ctggtctatg
      721 gcacgcttat ggcacatccg gaagttaagg taatttcaaa gctgcctacg tcagtgaccg
      781 tggcagtgga aaagggccag tgccacgaat ttgcggtgcc cagaaaagac ctcaagcgct
      841 tcgatccgca gagcgggctc aaggttacgg cggaatagga aagaagaatg agcaatccca
      901 ccgcagcatt gcaggcgcgc agcccgcttc tggaagagga aaagtggctg gggcgtttga
      961 tgctcgcccc ggcggtgatt tacatcgtcg cgctggtggg ttttccgttc ctgctcgcca
     1021 tttattacag cctgagcgac gccacggtgg gaagccaaac gctgcatttt gtgggtttca
     1081 ggaatttcca gcgtatcctc gaaaacagca ctttttggac ctcgctgcgc aatgccctgg
     1141 tgttcacctt cgtctcgcaa ctgctggtgg tgatcctcgg caaggcgctg gcgatggctt
     1201 tgtacaagga tttccgtggc aaatggctgg tgcgctttct gattctgctg ccgtgggtcg
     1261 cgccgatttc actcggcagc atcggctggc tgtggatttt cgatcccata tacagcatta
     1321 tcaattggac agcgcgggcg atcggcctct tcggccccca tacctggccg atctggctcg
     1381 gccagccgaa tctggcgatg gcgtccgtca tcatggtcga cgtatggcgc ttgctgccgc
     1441 tggccacggt catcatcctc gccggcttaa gctcgattcc gcaggacatc cacgatgcgg
     1501 cagaggtgga cggcgcgagt ttctggcggc atctcactta catcaacatc ccgctgttga
     1561 tgccgatcat gctggtcgcg ctcctgttcg gcatcgtatt tacttttacc gacatgattg
     1621 tgatatacgt gctgacccgc ggcgggccgt atgacacgac gcaagtgatt gccagcctcg
     1681 cctatttcac cggcatccag ggtggcgacc ttgccgaggg cgcggcgatt tcgctgttcc
     1741 tgttcccgct actagtcgcg gccgccatat tgttgctgac gttcgccaag cgcaccgagg
     1801 tgacctgaat gcaacgactc aagaacatcg gcggtcggat cggtcatttc ggcatcatcg
     1861 ccgcgtttac cgctttcagc gcgtttccgt tttactggat gttgatcacc accttcaagc
     1921 aaaccaatga tctcatcaac gtcaagaaca atccttttat ctacaacagc ccgccgacgc
     1981 tggagcactt gcgcatcctg cttgtcgaca cccagtacac gcaatggctg ttgaacacgc
     2041 tgggcgtcgg cgtgctggtg gtgctgatta cgcttctgct tgcggtgccc gcgggctaca
     2101 gcctggcgcg gctggcgggg cgctggggcc agcagctggc aatcggaata ttcctgactt
     2161 atctcgttcc gccgacgata ttgttcattc cgttttcgcg gattgtcgcc gatttaggct
     2221 tgcaggattc gctgtggtcg ctggtgctgg tttatcccag cttcaccgtg ccgttctgca
     2281 cctggctaat gatgggtttc ttcaagtccg tgcccaagga catcgaggag gcggcgatga
     2341 tagacggtct cagccgtttc ggcgcttttc tcaaggtggt ggtgccgatt tcctcggcgg
     2401 gaattctcac cgtcatcatc ttcacgctga cgctggtgat gcaggaattt gtgtacgcgc
     2461 ttaccttcat cacctcttcc tcgcagctca cggtcagcat cggcgtgccg accttcctgg
     2521 tgcgcggcga cgtgtatttc tggggctcgc tgatgggcgc gtgcctgatt gtgagcattc
     2581 ccatcgccat actgtataat ttcttcgtcg accgctttgt ggcgggcttt accgtgggag
     2641 ccgttaaata gtcgcaagcg caaaacgttt cgcctttctg ttttgtatcg cgctgctttt
     2701 cccaggcaac ggttacgctg ccccgctaaa tccggaaata gaagccttca tccgcgagat
     2761 ggcggaaaaa catcatttca atccgcggca gctgcgccga atcttcagcc aggtcaaacc
     2821 ccagcctgag gtgctcaagg cgataaccgc tcccccaacg gcgctgccct ggaatgagta
     2881 ccgcgcgttg tttatcaacc cggcgcgcat caccgacggc gtcaaattct gggacgagca
     2941 cgaaacagcg c
//
LOCUS       DATIOW010000161         8761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_578828, whole genome shotgun sequence.
ACCESSION   DATIOW010000161 DATIOW010000000
VERSION     DATIOW010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8761
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578828"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VLL03_05345"
     CDS             complement(<1..954)
                     /locus_tag="VLL03_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HSS46822.1"
                     /translation="MSFLQELFGPQWWPYVVTFSKTLALIVAIVVPLLLGVAYLTLAE
                     RKVIGYMQVRLGPNRVTFFGIPWLGGWAQPIADAFKLLAKEIIIPSGANRFLFVLAPV
                     MAIMPALAAWAVVPFQPELVLANIDAGLLYILAITSMSVYGVIIAGWASNSKYAFIGA
                     MRSAAQMVSYEIAMGFALVGVLMAAHSLNLSEIVKVQQSGAGFFSWFFIPLFPLFLVY
                     LISGVAETNRAPFDVAEGESEIVAGFHVEYSGMAFAVFFLAEYANMILVSALCSVMFL
                     GGWLPPFNFAPFTWLPGIFWLVFKVLFILFCFLWFRATFPRY"
     gene            complement(951..3293)
                     /gene="nuoG"
                     /locus_tag="VLL03_05350"
     CDS             complement(951..3293)
                     /gene="nuoG"
                     /locus_tag="VLL03_05350"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HSS46823.1"
                     /translation="MLELEIDGKQIEVTEGSTIMDAANRLGIFIPYFCYHRKLSIAAN
                     CRMCLVQVEKAPKPLPACATPVSGGMKVFTHSDEAVNAQKGVMEFLLINHPLDCPICD
                     QGGECPLQDLAVGYGGSSSRYQEEKRVVVNKNLGPLIATDMTRCIHCTRCVRFGQEIA
                     GVMELGMVGRGEYVEIMPFVEKTVDSELSGNMIDICPVGALTSKPYRYTARAWELSRH
                     KSVSPHDSFGSNLIVQVKENKVMRVLPRENEEINECWLSDRDRFSYEGLNSEERLTRP
                     MLKQDGEWKEVEWQTALEYIANRIKQIKNSHSAQAIGALATAHSTLEELYLLQKLVRG
                     LGSGNVDFRLRQSDFSADDARQGAPWLGMKIADLNSLDRVLVIGSALRKDHPLTAHRL
                     RQTVKRGMQLNLINPADDELLMPVANRIIVAPGMMNRALAEVLKATVDARNSSYPAQL
                     EEIQPSQEAKRIAESLLSGKNVAIFLGNIAQHHPQAAALHALAQELAMLTGAKFGFFG
                     EAANRLGGYIAGAVPDNGAGKPAKAMLDNPLKAYLLLNAEVELDCHDPQQALAAMRSA
                     ELVVAMSAYTHQAVEYAHVLLPIAPFTETAGTFINTEGRAQSFNSTVKPLGETRPAWK
                     VLRVLGNLLGLAGFEQESIEQVRVEVLPNGSVDPSRLNNGLRRLVSAGIEAPGSSGLQ
                     RIAEVPIYQADAIARRAASLQKTRDAAAPAAWLNGKLFERLGLREGDKVKLAQGRGIA
                     VLIARRDDKLPENCVRAASAHPLTANLGAMFGEITVERCA"
     gene            complement(3301..4596)
                     /gene="nuoF"
                     /locus_tag="VLL03_05355"
     CDS             complement(3301..4596)
                     /gene="nuoF"
                     /locus_tag="VLL03_05355"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HSS46824.1"
                     /translation="MTCLHGRHLNPIILAGLDGKNWRIGDYIRRGGYSALKKILSEKI
                     PPEQIINEVKKSALRGRGGAGFPTGLKWSFMPKSFAGVKYLVCNSDEGEPGTFKDRDI
                     MRYNPHIFIEGMAIAAYAMGVKVGYNYVHGEVWDCFERMEEAVEEARDAGFLGDNILG
                     TDFSFQLHNHHGYGAYICGEETALLESLEGKKGLPRFKPPFPASFGLYGKPTTINNTE
                     TFAAVPYIILNGGEKFLSTGKPNNGGTKIFSVSGHVNNPGNYEVPLGTPFAKLLELAG
                     GMRGGRSLKACVPGGSSMPVLPAHIMLATDMDYDSIVKAGSMLGSGAVIIMDETVCMV
                     RALLRLSYFYFEESCGQCTPCREGTGWLYRVVNRIEHGRGKQEDLDLLLNVADNIAGR
                     TICALGDAAALPVKSFITHFRDEFQHHIEHQQCLVPAYA"
     gene            complement(4593..5069)
                     /gene="nuoE"
                     /locus_tag="VLL03_05360"
     CDS             complement(4593..5069)
                     /gene="nuoE"
                     /locus_tag="VLL03_05360"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HSS46825.1"
                     /translation="MLSPEALAKIDKAIAKYPPDRKQSAVIAALTIAQDEKGWLSTET
                     MDFVAQYLSMPPIAVYEVATFYTMYNLEPAGKYKITICTNLPCALSGANIAADYLKNK
                     LGVDFNETTADGRFALKQGECLGACGDAPVLLVNNKRMCSFMSREKLDQLLKELKQ"
     gene            complement(5081..6334)
                     /locus_tag="VLL03_05365"
     CDS             complement(5081..6334)
                     /locus_tag="VLL03_05365"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HSS46826.1"
                     /translation="MAEIRNYTMNFGPQHPAAHGVLRLVLELDGEVIQRADPHIGLLH
                     RATEKLAEHKTYLQSVPYMDRLDYVSMMCNEHAYVMVIEKLLGLEVPLRAQYIRVMFD
                     EITRILNHLLWLGAHALDVGAMTVFLYAFREREDLMDCYEAASGARLHAAYYRPGGVY
                     RDLPESMPQYQASKIHSAAEVAKLNANRQGSLLDFIEDFTNRFPQYVDEYETLLTDNR
                     IWKQRTVGIGVVSAERARALGFTGPMLRGSGVEWDLRKKQPYEVYERMEFDIPVGVNG
                     DCYDRYLVRIEEMRQSNRIVRQCIDWLRKNSGPVITDNHKIAPPSRLEMKQNMEELIH
                     HFKLFTEGFHLPEGEAYAAVEAPKGEFGIYLVSDGANKPYRLKIRAPAFAHLAALDEM
                     ARGHMIADVVAIIGSQDIVFGEIDR"
     gene            complement(6327..6941)
                     /locus_tag="VLL03_05370"
     CDS             complement(6327..6941)
                     /locus_tag="VLL03_05370"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HSS46827.1"
                     /translation="MATKLENLRACLNAVLGERIKNFSEAVGEITLTVKPADWLTVAQ
                     TLRDNPDLRFEQLIDLCGVDYQDYGDGAWNGPRFAVVCHVLSLTHNWRLRVKAFAEDD
                     QFPVFDSLISVWPCANWYEREAFDLFGMVFTGHPDLRRILTDYGFIGHPFRKDFPLSG
                     NVEMRYDPDLQRVIYQPVTIDPRNNVPRVVREENYADSEGFRNG"
     gene            complement(7233..7709)
                     /locus_tag="VLL03_05375"
     CDS             complement(7233..7709)
                     /locus_tag="VLL03_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HSS46828.1"
                     /translation="MDTQGVLEKGFVTTKLDDLINWTRTGSLWPMTFGLACCAIEMMH
                     AGASRYDLDRFGIVFRPSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGIIQLQNKIKRTNTIAR"
     gene            complement(7712..8086)
                     /locus_tag="VLL03_05380"
     CDS             complement(7712..8086)
                     /locus_tag="VLL03_05380"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010102655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HSS46829.1"
                     /translation="MLENYFPILLFIIVGLAVGVLPMMIGWLLSQPGLLGTNRPDSEK
                     LSPYECGFEAFEDARMKFDVRYYLIAILFIVFDLEIAFLFPWAVVLKEIGMFGFVSMM
                     IFLAILVVGFIYEWMKGALEWE"
     gene            complement(8232..8316)
                     /locus_tag="VLL03_05385"
     tRNA            complement(8232..8316)
                     /locus_tag="VLL03_05385"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8280..8282),aa:Leu,seq:gag)
     gene            complement(8407..8760)
                     /gene="secG"
                     /locus_tag="VLL03_05390"
     CDS             complement(8407..8760)
                     /gene="secG"
                     /locus_tag="VLL03_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HSS46830.1"
                     /translation="MLETLIWILHVLTALAIIGLVLLQHGKGADAGAAFGSGASGSLF
                     GATGSANFLSRTTAILAAVFFVTSLGLTYLSSHKGDSKGVMELHKEVPPAASPVSKPA
                     PPAAEGDSKAKDIPK"
BASE COUNT         1873 a         2639 c         2460 g         1789 t
ORIGIN      
        1 gtagcgcggg aaggtggcgc ggaaccagag gaagcagaac aggataaaca gcaccttgaa
       61 caccagccag aaaatcccgg gcagccaggt gaacggcgcg aaattaaacg gcggcagcca
      121 gccaccgagg aacatgacgg aacacagagc cgagaccagg atcatgttgg cgtactcggc
      181 gaggaagaac accgcaaacg ccatgccgga atactcgacg tggaagccgg cgacgatttc
      241 cgattcgcct tccgccacgt cgaatggggc gcggttggtt tcggctacgc cggaaatcag
      301 gtacaccagg aacaacggaa acagcggaat gaaaaaccag ctgaaaaacc ccgcgccgct
      361 ttgctgcacc ttgacgattt ccgacaaatt cagactgtgc gcagccatca acacgcctac
      421 caaggcaaaa cccatggcga tttcgtagga aaccatttgc gccgccgagc gcatcgcgcc
      481 gataaaggca tatttggagt tggatgccca gccggcgatg atcacgccat acacgctcat
      541 ggacgttatc gccaagatgt agagcagccc ggcgtcaatg ttggcgagca ccagttcggg
      601 ctgaaacggc accaccgccc acgccgccag cgccggcatg atagccatca ccggcgccag
      661 cacgaacagg aagcggttgg cgccgctggg gatgatgatt tccttggcga gcagcttgaa
      721 cgcatccgcg atcggctggg cccacccacc gagccatggg attccaaaga aagtcacccg
      781 attcgggccg agccgcacct gcatgtaccc aatcaccttg cgctctgcga gcgtcagata
      841 tgcgacgccc aacaacagcg gcaccactat ggcaacgatc aaagccaggg tcttggagaa
      901 agtcacgaca tacggccacc actgtggccc gaagagttcc tgcaggaagc tcatgcgcaa
      961 cgctccacgg tgatttcgcc aaacatcgcg ccgagattcg ccgtcagcgg atgcgcactg
     1021 gccgcccgca cgcagttttc gggcagcttg tcgtcgcggc gcgcaatcaa caccgctatt
     1081 ccccgccctt gagcgagttt aactttatcg ccttcgcgca aacccaagcg ttcgaacagt
     1141 ttgccgttga gccaggccgc cggcgcagcc gcatcgcggg tcttctgcag cgaagcagcg
     1201 cggcgcgcaa tggcgtcggc ctgataaatc ggcacttccg cgatccgctg caaaccgctt
     1261 gagcccggcg cttcgatacc ggcagaaacc agccggcgca ggccattgtt caggcgcgag
     1321 ggatccacgc tgccgttggg caggacttcc acccgcacct gctcgatgct ttcctgctca
     1381 aagccggcaa ggccaaggag attccccaac acgcgcagca ctttccatgc cgggcgggtt
     1441 tcacccagcg gcttcaccgt gctgttgaag ctttgcgccc gaccttcggt gttgataaag
     1501 gtgccggcgg tctcagtgaa aggcgcgatc ggtagcagca catgcgcgta ttcgaccgcc
     1561 tggtgcgtat aagcgctcat cgccaccacc agttcggccg agcgcatcgc ggcaagcgct
     1621 tgctgcggat catggcaatc gagctcaact tcggcgttaa gcaagagata agccttgagc
     1681 ggattgtcca acatcgcttt tgccggcttg ccggccccgt tatcaggcac cgcgccggcg
     1741 atgtagccac ccaacctgtt tgccgcttcg ccaaagaaac cgaatttcgc gccggtaagc
     1801 attgcaagct cttgagcaag cgcatgcaaa gcagccgcct gcggatggtg ctgcgcgatg
     1861 ttgccgagga atatcgcgac gttctttccc gagagcaggc tttccgcaat gcgcttcgct
     1921 tcctgcgaag gttggatttc ctcaagctgc gccgggtagg atgaatttct cgcgtccacc
     1981 gtcgctttca gcacttccgc caacgctcga ttcatcatgc ccggcgcgac aatgattcgg
     2041 ttcgccaccg gcatcagcaa ctcatcgtcc gccggattga tgagattgag ctgcatgccg
     2101 cgcttcaccg tttggcgcag acggtgggcg gtaagcggat ggtccttgcg cagcgcgctg
     2161 ccgataacca gcacgcggtc gagtgaattg agatcggcga ttttcatccc cagccagggc
     2221 gcgccctgcc ttgcgtcatc tgcgctgaaa tcggattgcc gcaaacggaa atccacattg
     2281 ccgctgccca gaccgcgcac cagcttttgc agcagataaa gttcctccaa ggtgctgtgc
     2341 gcagtggcga gcgcgcctat cgcttgcgcg ctatgtgaat ttttgatttg tttaattctg
     2401 tttgcaatgt attccagcgc cgtctgccat tccacttctt tccactcgcc gtcttgcttc
     2461 agcatcggcc gggtcaggcg ctcctccgaa ttcagtcctt cgtaggaaaa gcgatcccgg
     2521 tcggaaagcc agcattcgtt gatttcctcg ttctcgcgcg gcagcacgcg catgactttg
     2581 ttttccttca cctgtacgat aaggttcgaa ccgaaactgt cgtgcgggct caccgactta
     2641 tggcgcgaca attcccaagc gcgcgcggtg tagcggtaag gttttgacgt gagcgcgccc
     2701 accgggcaga tatcgatcat gttgcccgag agctccgaat ccaccgtttt ttcgacgaag
     2761 ggcatgattt ccacatattc gccgcgcccc accatgccga gctccatgac gccggcgatt
     2821 tcctggccga agcgcacgca acgcgtgcag tggatgcagc gcgtcatgtc ggtggcaatc
     2881 agcggcccca agttcttgtt caccactacg cgcttttctt cctgataacg cgaggaactg
     2941 ccaccgtagc ccaccgcgag atcctgcagc gggcattcgc cgccttggtc gcagatcggg
     3001 cagtcgagcg gatgattaat cagcaaaaat tccatcacgc ccttctgcgc attcaccgcc
     3061 tcgtcggaat gagtaaacac cttcatcccg ccgctgaccg gagtggcaca ggcaggcagc
     3121 ggcttgggcg ctttttccac ctgcaccaaa cacatgcggc agttggcggc gatagaaagc
     3181 tttcggtgat agcagaaata cggaatgaaa atgccgagcc ggttggcggc atccatgatg
     3241 gtgctgcctt ccgtcacttc gatctgcttg ccgtctattt caagctctaa catgattatt
     3301 ctatgcgtaa gcggggacaa gacattgttg gtgctcgatg tggtgctgga actcgtcacg
     3361 gaaatgggta atgaagcttt tcaccggcag cgcggcggcg tcgcccagtg cgcaaatggt
     3421 gcgccccgcg atgttgtcgg cgacgttgag cagcagatcc agatcttcct gttttccccg
     3481 gccgtgctcg atgcggttca ccacccgata cagccagccg gtgccttcgc ggcacggcgt
     3541 gcactggccg caggattcct cgaaataaaa ataggaaaga cggagcagcg ccctcaccat
     3601 gcacaccgtt tcatccatga tgatgaccgc gcccgagccc agcatcgagc ccgccttgac
     3661 gatggaatcg taatccatgt cggtggcgag catgatatgc gcgggcaata ccggcatcga
     3721 agagccgccg ggaacgcagg ccttgaggct tctcccgccg cgcatgccgc ccgccagttc
     3781 gagcagtttc gcaaacggcg tgcctagcgg cacttcatag ttgcccgggt tgttcacgtg
     3841 gccggagact gaaaaaatct tggtgccgcc gttattgggc ttgccggtgc ttaggaattt
     3901 ttcgccgccg ttcaaaataa tgtagggaac ggctgcaaag gtttcggtgt tgttgatggt
     3961 ggtgggtttc ccgtacaagc caaagctcgc gggaaaaggc ggcttgaagc gcggcaggcc
     4021 tttcttgcct tccagcgatt ccagcaacgc ggtctcttcg ccgcagatgt aggcgccgta
     4081 gccgtgatga ttgtgaagct ggaagctgaa atcggtgccc agaatattgt cgccgagaaa
     4141 acccgcgtcg cgcgcctctt ccaccgcctc ttccatgcgc tcgaagcagt cccacacttc
     4201 gccatgcacg taattgtagc cgactttcac gcccatcgcg tacgccgcaa tcgccatgcc
     4261 ctcgatgaag atatgcgggt tatagcgcat gatgtcgcgg tccttgaaag tgcccggctc
     4321 gccctcgtcg gaattgcaca ccagatactt gacgccggcg aaacttttcg gcatgaagct
     4381 ccacttaagc ccagtcggga aacctgcgcc gccgcgtccg cgcagcgcgg atttttttac
     4441 ttcgttgatg atctgctccg gagggatttt ttcagacagt attttcttca gcgccgaata
     4501 gccgccgcgc cggatgtaat cgccgatgcg ccagttcttg ccatcgagtc cagcaagaat
     4561 gatcggattg aggtggcgac cgtgcaggca ggtcattgct tcaactcctt gagcaactgg
     4621 tccagctttt cccgcgacat gaagctgcac atgcgcttgt tgttgacgag cagcaccggc
     4681 gcatcgccgc aggcgcccag gcactcgccc tgtttcagcg caaaccggcc gtccgccgtg
     4741 gtctcgttga aatccactcc gagtttattc ttcagataat ccgccgcgat attggcgccg
     4801 gaaagcgcgc aaggcagatt ggtgcaaatg gtaatcttgt atttgccggc gggctccagg
     4861 ttgtacatgg tgtaaaacgt cgccacctcg tacaccgcaa tcggcggcat gcttaaatat
     4921 tgcgccacga aatccatggt ctcggtggac agccatccct tctcgtcctg cgcgatcgtc
     4981 agcgccgcta tcaccgccga ctgttttcgg tcaggtggat atttggcgat ggctttgtcg
     5041 attttggcga gcgcctctgg gctcaggatc atgggcgcat tcatcggtca atttccccaa
     5101 acacaatgtc ctggctccct ataatcgcca ccacgtctgc aatcatgtgg ccgcgcgcca
     5161 tttcgtcaag cgccgccaga tgcgcgaagg ctggcgcgcg gattttcagg cgataaggct
     5221 tgttggcgcc gtcggaaacg agataaatgc cgaattcgcc cttgggcgct tccaccgcgg
     5281 cataagcctc gccttcgggc agatggaaac cctcggtgaa gagcttgaaa tggtgaatca
     5341 gttcttccat gttctgcttc atctccagac gcgagggcgg cgcaatcttg tggttgtcgg
     5401 tgatcaccgg gccggaattt ttcctaagcc agtcgatgca ctgcctcaca atgcggttcg
     5461 actggcgcat ttcctcgatg cgcaccagat accggtcgta acaatcgccg ttcacgccca
     5521 ccgggatgtc gaattccatc cgctcgtaga cttcgtaagg ctgcttcttg cgcaagtccc
     5581 actccacccc cgagccgcgc agcatcggac cggtgaagcc gagcgccctg gcgcgctccg
     5641 cagacaccac accgatgcct acggtgcgct gcttccagat gcggttgtcg gtgagtagcg
     5701 tttcgtattc atccacgtat tggggaaagc ggttggtgaa atcctcgatg aaatccagca
     5761 gcgagccctg gcggttggcg ttgagtttcg ccacctccgc ggcactgtgg attttcgaag
     5821 cctggtattg cggcatgctc tccggcaagt cgcggtacac accgcccgga cggtaataag
     5881 ccgcatgcag gcgcgcgccg gaagccgctt cgtagcaatc catcaggtcc tcgcgctcgc
     5941 ggaaggcata gagaaacacc gtcatcgcac cgacgtcgag ggcgtgcgcc ccgagccaca
     6001 gcagatggtt gagaatgcgc gtgatttcgt cgaacatgac gcggatgtat tgcgcgcgca
     6061 gcggcacctc gaggcccagc agtttttcga taaccatgac gtaggcatgc tcgttgcaca
     6121 tcatcgacac gtagtcgagc cggtccatgt acggcaccga ctgcaggtag gtcttgtgct
     6181 cggcgagctt ttcggtcgcg cggtgcagca gcccgatatg cgggtcggcg cgctggatca
     6241 cttcgccgtc aagctccagc accagccgca gcacgccgtg cgccgccggg tgctgcggcc
     6301 caaagttcat ggtgtaattt cggatctcag ccattcctga atccttcgga atcggcgtaa
     6361 ttctcctcgc gcaccacgcg cggcacattg ttgcgcggat ctatcgtcac cggctgatag
     6421 atcacccgct gcaaatccgg gtcgtagcgc atttccacat tgccggagag cgggaaatcc
     6481 ttgcggaacg gatggccgat aaaaccgtaa tcggtaagga tgcgacgcaa atcgggatgg
     6541 ccggtaaaga ccatgccgaa caaatcaaat gcctcgcgtt cgtaccagtt ggcacagggc
     6601 catactgaaa tcagcgaatc gaacacgggg aactgatcat cctcggcaaa cgctttaacc
     6661 cgcagccgcc agttgtgagt gagcgacagc acgtggcaca ccaccgcaaa gcgcggcccg
     6721 ttccacgcgc catcgccata atcctgatag tccacgccgc agaggtcgat caattgctcg
     6781 aaacgcaggt cggggttgtc acgcagcgtt tgcgctacag tcagccaatc cgcaggtttg
     6841 acggtaagtg tgatttcgcc caccgcttcg ctgaagtttt tgatacgctc gcctaaaaca
     6901 gcgttaaggc acgcgcgaag attttccagt ttggtggcca ttaaattaga tgtctgaatg
     6961 aataaattga gggctctgca aaacaagaat ttggtttcga gacgaggcgc aagctttggt
     7021 cgaagggcgc cgcgacacgg ttgagccgaa tacgggagca gcgacaaaca ccctgggcaa
     7081 gcactgccat ttgctgcgcc cagctaccct gtctccgccc cgagcttcgc cgccgtgagc
     7141 cagggtgcag tgtatatgag aaatacatga gcattcgagc aaagcgagca acaccgtatc
     7201 ggagccaaag acgccgtttt gcagaggtct catcagcggg caatggtgtt agtgcgcttt
     7261 atcttgtttt ggagctggat gattccgtac agcagcgctt ccgcggtggg cgggcagccg
     7321 ggcacgtaaa tgtcaacagg cacgatgcgg tcgcagccgc gcaccaccga gtacgaatag
     7381 tgatagtagc cgccgccgtt agcacaggag cccatggaaa tcacccagcg cggctcggcc
     7441 atttggtcgt acacttttct gagcgccggc gccatcttgt tgcacagcgt gccggcgacg
     7501 atcatcacgt ctgactggcg cggactgggg cggaacacga tgccgaagcg gtcgagatca
     7561 tagcgcgaag cgccggcgtg catcatttct atcgcacaac aggcgagtcc gaaggtcatc
     7621 ggccacagcg atccggtgcg cgtccaattg atgaggtcat ccagcttggt cgtgacaaag
     7681 cctttttcca gcacgccctg tgtatccatg ttcattccca ctctaacgca cccttcatcc
     7741 actcatagat aaagccgacc accaggatgg cgaggaaaat catcatcgag acaaaaccga
     7801 acattccaat ttccttgagc accaccgccc agggaaaaag aaaggcgatt tccaggtcga
     7861 acacaatgaa cagaatggcg atgaggtaat agcgcacgtc gaacttcatg cgcgcgtcct
     7921 caaacgcttc gaatccgcac tcgtaggggg aaagcttttc gctgtcgggg cggttggtgc
     7981 ccaaaagccc gggttgcgac agcaaccagc caatcatcat cgggagcacg ccgacagcca
     8041 gcccgacaat aatgaacagc aagattggaa aatagttttc cagcatgttt acttcaggat
     8101 tgaggattca agattctgga ttcaatcaaa gggactccgt cagtccagcc gttaatttat
     8161 gacccctaat gttgcttcaa gttcggaata tcatgaccgg tgcggcactt aactcctgaa
     8221 tcctgcattc gtggtgccga tgatgagact cgaactcata cggctctcgc caccgccccc
     8281 tcaagacggc gtgtctacca attccaccac atcggcgcaa aaccccgtga ggggtaagga
     8341 gtaaagggta aggggaaaat cctgaagcat gattagtttt tactcctcac acctcactcc
     8401 tcacggctac ttcggtatgt ccttggcctt ggaatcgccc tcggcagccg gtggcgcagg
     8461 cttgctgacc ggcgacgccg ccggtggtac ttccttatgc aactccatca cacccttgct
     8521 gtcgcccttg tggctggaga gataagtcag ccccaggctg gtgacaaaaa acaccgccgc
     8581 cagtatcgcc gtggtgcggc tcaggaaatt cgcggaaccc gtggcgccga aaaggctgcc
     8641 ggaagcaccg ctgccaaaag ccgccccggc atccgcacct ttgccatgct gcagcagcac
     8701 caagccgata atggcgagcg cggttaacac gtggagaatc caaatcaaag tttccaatat
     8761 t
//
LOCUS       DATIOW010000162        15732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_783990, whole genome shotgun sequence.
ACCESSION   DATIOW010000162 DATIOW010000000
VERSION     DATIOW010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15732
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783990"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VLL03_05395"
     CDS             <1..326
                     /locus_tag="VLL03_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46831.1"
                     /translation="RLNQPSYRRFKMFTNRNKLSALLLSTLLSISALQPARAANFTEA
                     DAKACEWLDQQLRMTDGGSPASAFCNGPASVYQGNSMTVSSATEKTDNSPDTWYERQL
                     RIQHG"
     gene            1294..2199
                     /locus_tag="VLL03_05400"
     CDS             1294..2199
                     /locus_tag="VLL03_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSS46832.1"
                     /translation="MNKQMDRRQFLKLAGLGGVVFVSGLAGCASAGRKPGATAYDDFY
                     FVQLSDTHWGFEGAAVNPDAKGTLKKAVAAVNGLDDKPDFIVFTGDLTHTTDDPKIRR
                     QRLAEFREIVSELKVKNVRFMPGEHDASLDRGEAYQEFFGKTHYTFDHKGVHFIVVDN
                     VSDPGAKIGDEQLQWLSADLQQQDKNARIVVFTHRPLFDLYPQWDWATRDGAKAVELL
                     MPYNNVTVFYGHIHLENHHMTGHIAHHSAKSLIFPLPAPGSQPKREPLPWDVSQPYRG
                     LGFRSVEAEVDKADYAIIEYPVLKG"
     gene            2236..2568
                     /locus_tag="VLL03_05405"
     CDS             2236..2568
                     /locus_tag="VLL03_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HSS46833.1"
                     /translation="MLFLALGSSLGNYVTAEPTERVIKITAKAFEYTPNEITLKKGEP
                     VVLELTSQDLFHGFNITDLGLRADLPPGMTARVRLTPDKTGNFEFHCDNFCGTGHENM
                     TGVIKVVE"
     gene            2616..3185
                     /locus_tag="VLL03_05410"
     CDS             2616..3185
                     /locus_tag="VLL03_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HSS46834.1"
                     /translation="MQAFLTSAALIAVAEIGDKTQLLSFVLAARLRRPYPIIAGILSA
                     TLFNHTLAGSLGVWLAKLVPHSFLNWIVALSFIAFGLWTLHPDSLEEDPRIHEAGVFV
                     TAFIAFFLAEMGDKTQLATIALAARFDALVEVVLGTTLGMLLADVPAVFIGEKLAKYI
                     PMRLVRYAAATLFIALGLFTLMRPLELGA"
     gene            3262..4086
                     /locus_tag="VLL03_05415"
     CDS             3262..4086
                     /locus_tag="VLL03_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HSS46835.1"
                     /translation="MWLKSLLLFSILFAFLAFPAIAANQTEALLDQVINGAHRDPKNK
                     ARDIYRHPKETLLFFGLKPNMTVVEIWPGGGWYTEILAPVLRDQGKLYAASFAVNAKN
                     SPEFRIEVERDYLKKLSQHPEIYGRIIHTQLQAPEYVNIGPHGKADMVLTFRNVHNWV
                     KDGNAEATFKAFYDVLKPGGILGVIDHRAKPGTPLQDMIRSGYVTEELVIGLAEGAGF
                     KLAAKSEINANPKDTKNHPKGVWTLPPMLRLGDQDKQKYLAIGESDRMTLKFVKPK"
     gene            complement(4088..5596)
                     /locus_tag="VLL03_05420"
     CDS             complement(4088..5596)
                     /locus_tag="VLL03_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HSS46836.1"
                     /translation="MKTKALFFSLLLLAPAAFAEATLNIGSKRFTESYVLGEILARAA
                     ANANEAKVVHKQGLGNTGILFAALRSGSIDVYPEYSGTIWFELLKKNAAGDLAALNRE
                     LAPLGLAAAVPLGFNNTYALAMPDARARQLGISSIGDLARKPELKLGLSQEFLNRQDG
                     WPPLKKAYALPFDPLGLDHGLAYEALVKGKIDVIDIYSTDAKIAKYGLRVLSDGKHFF
                     PAYDALLLYRLDVPGRFPKTWAALQKLEGRISETQMAAMNAEAELTGKPFAAIAADFL
                     SSGAGHKPTESTGRNFLTLLFAADFWHLTAEHLLLVFVSLGGAILLGLPLGVCAAYSP
                     VARQPILSAVGVIQTLPSLALLAFLISALGTIGVLPALIALFLYALLPIVRNTYAGLA
                     DIAPSLQEAAQSLGLPLLPRLRLIELPLASRAILAGIKTSAVINVGTATIAAFVGAGG
                     YGERIVRGLALNDNATLLAGAIPAAALALLVQGGFELLDRWLIPAGLRERKG"
     gene            complement(5596..6333)
                     /locus_tag="VLL03_05425"
     CDS             complement(5596..6333)
                     /locus_tag="VLL03_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSS46837.1"
                     /translation="MIAVENVCKSYGRQIALAPTSLAFHAGTTALIGESGCGKSTLLR
                     LMLGLIRPDSGRIVFNTEPLTADNAERVRHAIGYVIQDGGLFPHLTARDNVTLLARFL
                     RRAQSWIDERVNHLADLVRLQPAALARHPRDLSGGQRQRVGLMRALMLDPEALLLDEP
                     LGALDPIIRSELQTELKSIFAQLAKTVIFVTHDMNEAAYLASDIVLLREGRVIQRGAL
                     NDLLHRPAEPYVREFIHAQRSLITETA"
     gene            6461..7324
                     /locus_tag="VLL03_05430"
     CDS             6461..7324
                     /locus_tag="VLL03_05430"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSS46838.1"
                     /translation="MDSQFLSLKPKTEFTRREFVVTLLASGFALAVQPVSAQTLHTDD
                     KGLTAGEVKIPVNDGEIPAYRAMPAGGGPFPVILVVQEIFGVHEHIKDVCRRFAKLGY
                     FAVAPELYSRQGNVSKLSDIQQILSDVVSKVPDAQVMSDLDATVAWAKKSGKGNTAKL
                     GITGFCWGGRIVWLYAAHNSKLKAGVAWYGPLLWKTNKLQPKNPIDMAGLLKAPVLGL
                     YGGADPGIPLDAVEALRKALKAAGSKSEIVVYPDTPHGFHADYRPSYRKAQAEDGWKK
                     LQDWFKTHGVA"
     gene            7334..7753
                     /locus_tag="VLL03_05435"
     CDS             7334..7753
                     /locus_tag="VLL03_05435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HSS46839.1"
                     /translation="MPCELCESAGGEPLWRDVLCRVVLVNDAEHPGFCRVIWNAHVKE
                     MTDLGETERQHVLRVALAVETALRKLLSPDKINLASLGNMTPHLHWHVIPRFHDDPHY
                     PNPVWGRKLRAGPRVLPRGFRDTMQAALIRALGSSIN"
     gene            7775..9379
                     /locus_tag="VLL03_05440"
     CDS             7775..9379
                     /locus_tag="VLL03_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46840.1"
                     /translation="MLPMSLPDTHPSAPCDWKDAASCKHWLDQLPLTNFGQAQQALTA
                     ELKRFLCLEMAPLERLNTLEQLRETAAMVQGECAKKYAAKPLSMAATEVTHWKQSIEL
                     WQNLRAAYQRCLLDALGGGGEAAQHAALICQRCLRYTTLTFFEYYLTNQEVDPELWRH
                     LHGLYAFAEKHNLTDEAVRDSLIEEAKTTSCTVAYAQALLIGRSNPYSLTPKQLELAS
                     RWINKWGEKIQVRTTRSEGESVLTVDPESNSGARQAGKIRDSSSTRYLVTDDLGKNLG
                     IRVKLLGEGKSPAELGLGSDCLQPGCEALLKLLYQQWCKQGGTVRAAPRHQADRKAQL
                     CFGIPAAHYFVSGKPFKQPGAPQLSRQEEEELRTFGHISKHREAMQLSQLGFALDSWK
                     IINDSSIGLGMIRDANGGGSRVTLKQLIGVCPAESKHFMLCVVEWITLQQNGELHIGV
                     RALPGLPEPVAVRASGTGTGAAYVQAFLLPDVSVLNAEPSLVLPNGWFQTGRIIDVFT
                     GESQRIKLIKLMEKGTDYERVDFGAP"
     gene            9474..9884
                     /locus_tag="VLL03_05445"
     CDS             9474..9884
                     /locus_tag="VLL03_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HSS46841.1"
                     /translation="MAGLDRNTPTPTEIKLHQQSRVMEITFSDGKTFKLPYEFLRVYS
                     PSAEVRGHGPGQEVLQVGKKNVDIIGIEPVGTYAVQPRFSDGHETGIYSWDLLYDYGV
                     NQEKMWGTYLKRLEAAGASREITAAPFGPGLKSK"
     gene            9944..10918
                     /locus_tag="VLL03_05450"
     CDS             9944..10918
                     /locus_tag="VLL03_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSS46842.1"
                     /translation="MLKTVGCALLIGSAIASVNAPAEDVVRLGNLKFAHYGAVSYMKE
                     IAPKYGLKIEERMFAKGIDILPAIVAGEIDASASALDGAIAGRAQGVPIYAVAGFAKG
                     GARIVARSDLAIKSVADLKGKKVGVARGGAQELLLFAELSKAGLTWSEKPGSDVQIVY
                     LAYADLNQALLGKDIDAMCQSEPQSSQAINKGFGIEVIKPYDTPMGEPVRSLVITEKL
                     YKERPQAAQKLLNVFVEATALFIAKPELAEKYVREQMFKNQITSQDFKDAIANSPYTY
                     DLTVEHVQITTDLMLKYGVGRLGANAPAAKDWVKLDLLENAKAALKLK"
     gene            10928..11755
                     /locus_tag="VLL03_05455"
     CDS             10928..11755
                     /locus_tag="VLL03_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSS46843.1"
                     /translation="MGVTSFRSILELARGVVIPVVVLALWQFVAMSGWVNPQILPAPS
                     QVVAKWWEYLRPTAPYSAGSYALWLVSGELPHDTLASLSRVVGGFLIGTLLALPLGLL
                     MGTSNRLYGLFNPLVQVLRPIPPIAYIPLAILWFGLGNPPAFFLISIGAFFPVLMNTI
                     AGVRNVDGIYLRAARNLGAGQMTLFRRVIVPAAMPYILTGMRIGIGTAFIVVIVSEMI
                     AVNSGLGYRILEAREYMWSDKIIAGMFTIGLLGLGIDIFMNGLNNWLLKWHRGIDQN"
     gene            11768..12562
                     /locus_tag="VLL03_05460"
     CDS             11768..12562
                     /locus_tag="VLL03_05460"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSS46844.1"
                     /translation="MFMHIENVNKRFPVPGGEVVALKDINLDVGEGEFVCLLGPSGCG
                     KSTLLNAIAGFSPPTSGSIAINSRADAARFPGPDRGMVFQEYALFPWMTVAQNIRFGL
                     EIKGLSKAEMNGKVDMLLRKLNLYEFRERFPKDLSGGMCQRVAIARVLALDSPIMLMD
                     EPFGALDALTRRTLQDELLRIWDEFRKTIIFVTHSIEESIYLADRVAVMTYRPGTIKR
                     DVRITMLRPRDSASAEFNALKKELAESVMEEQMRYTQTEIRGSTAE"
     gene            12603..13337
                     /gene="ubiE"
                     /locus_tag="VLL03_05465"
     CDS             12603..13337
                     /gene="ubiE"
                     /locus_tag="VLL03_05465"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HSS46845.1"
                     /translation="MSKTTDFGFQKVAEEEKTSRVTSVFNSVAGRYDLMNDLMSLGAH
                     RLWKRFAVELSAIKAGGRVLDIAGGTADLSTLFAKRTGESGEVWLTDINNSMLCIGRD
                     RLLDKGLALPVAQCDAEELPFPNDYFDCVSVAFGLRNMTHKEAALKEMQRVLRAGGRL
                     LVLEFSKIWKPLQPLYDVYSFKVLPALGKFVANDADSYKYLAESIRLHPSQEELKQMM
                     ESAGFERVEYFNLSAGVVALHRGYKF"
     gene            13574..14335
                     /locus_tag="VLL03_05470"
     CDS             13574..14335
                     /locus_tag="VLL03_05470"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HSS46846.1"
                     /translation="MKKFALILLAALLTVGLASLDAQAKRPGGGNVTKQHSTVTHKAE
                     PPAPPQASAPAPAPAKGMLAGLVSGGVLASLLLGAVFFIFRMLRRKSAAESLQYVALG
                     AETTPAMPARIFGSFLRPAPYPVGFEIEPFLRNAKASFTRLQAANDSADLDVIRDYTT
                     PKMFVALKKQIEARSSDPRKTDIVTLDAEVLEVAIEDDLAVASVRFSGLLREKDFGGP
                     AQPFYEIWNVQKRLHEPKSVWLLARIQQAHRIVHA"
     gene            14328..14888
                     /locus_tag="VLL03_05475"
     CDS             14328..14888
                     /locus_tag="VLL03_05475"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HSS46847.1"
                     /translation="MLDAAILNHLLRGESWARQRLETHTGKTARFLVPPFSFTLTVAE
                     NGEAKPAEDVATADVQFTMSPVLLTRLFARDEAAYKEVRIEGDTEFAGAIAYIAKNLR
                     WDPAEDLSRIVGDIAAQRIVNTAHSINNWGKRSLDSLARSLAEYWTEEQPLIAKTVDV
                     KKFVVGVDALRDDVERLEKRLHKLSN"
     gene            14927..>15732
                     /locus_tag="VLL03_05480"
     CDS             14927..>15732
                     /locus_tag="VLL03_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HSS46848.1"
                     /translation="MRLFRLLKIISISVRFGLDEFFLGHERVRLLRVLLKLLLFWRRL
                     EKPRAVRLRLALEALGPIFVKFGQVLSTRRDLLPADIADELAKLQDQVPPFPSAEAVA
                     LLERVYRRPLSDVFRDFEREPAASASVAQVHLAVLHDGTDVAVKVLRPGIAAVIAKDL
                     ALLDIGAGLIETLWRDGKRLKPHEVVREFEKHLHDELDLMREAANASQLRRNFENSPL
                     LIVPEVHWDYCANEVMVMQRMHGTPISQVQRLRDLGVDIPKLARAGVEIF"
BASE COUNT         3462 a         4527 c         4548 g         3195 t
ORIGIN      
        1 accgtcttaa ccaaccttca tataggagat tcaaaatgtt tacgaataga aacaaattgt
       61 cagctctttt attgagcacc ctgctttcga tctctgcgct tcagcctgca cgggcggcga
      121 atttcaccga ggcggatgcc aaagcgtgtg agtggcttga tcaacagctt cggatgaccg
      181 atggtggttc cccggcgtct gcattctgca acggtccggc ctctgtttat caagggaata
      241 gcatgactgt ttcctctgcc accgaaaaga cagacaactc gcctgacacg tggtacgagc
      301 gccaactcag aatccaacac ggctaaatct gggtggttta tttccggaat tccccgaagg
      361 caacttcggg gaatatttcg ggtgcgggtc tggttttgca ggtacaggcc ccttactttt
      421 gacacaacga gactcaaatc aaatagaggc gtgaaatgga cgactacgca attggactgc
      481 ttggggcaac catgatactt accgtggtga cggttttcgt aagccgcgct ctattactca
      541 gtcgccccga gcaaaattcc gaaggctccc aggcgaaaag ccaccggagc taagcagcgc
      601 tgcgaactgg gcttacctgc gtatttcaga ttccgacggt agcgtaggac agctctgtca
      661 acttgaagga aatcaaagtg cagggaatcg caagtagggg tgaggcgggg cacaaatcga
      721 ctccgaacgg ctggtgagag ctctgcgctc acacaccatt gcgattctgc attaccggat
      781 attggctcat tttattccct aaaccaaatt aaaacgaatt ccgggaatat tcgtcgcgcg
      841 tcgtaggtta tgcagggtgt agacggcttg attggcaaac cgtcttaacc aaccttcata
      901 taggagattc aaaatgctta cgaatagaaa caaattgtca gctcttttat tgagcaccct
      961 gctttctttc tccgcgcttc agccggcact ggcagcagaa gacgccaaaa tggcgcggaa
     1021 ttcaaagcag aacggcgacg ccccttgcgc ggaatccaag aaacaagtta acgacaatac
     1081 cggcaagaca aacgacagct ggtttgaccc agcatgctgc gagccaggta cgacatggta
     1141 cgagcgccaa agcaggatcc ccggcggcta ggaatacctc gaatctgggt ggtttgtttc
     1201 cggaattccc cgaagccaac ttcggggaat atttcggatg cgaatctagt tatgcaggtt
     1261 acggaccctt acttttgaca caaggagact caaatgaaca agcaaatgga ccggcggcag
     1321 tttcttaaac tggctggcct gggcggcgtg gtatttgttt ccggccttgc cggctgcgcc
     1381 agcgcgggaa gaaaaccggg cgcgacggct tacgacgatt tctattttgt gcagctttcg
     1441 gacacccatt ggggattcga aggcgcggca gtcaatcccg acgccaaagg caccttgaaa
     1501 aaagcggtgg cggcggttaa cggcttggat gataaacccg atttcatcgt tttcaccggc
     1561 gacctcaccc acaccaccga tgatcccaaa atccgcaggc aacggcttgc cgaattccgc
     1621 gagatcgtga gcgagcttaa ggtgaaaaac gtccgcttca tgccaggcga gcacgacgcc
     1681 tctctcgacc gcggcgaagc atatcaggaa tttttcggca aaacccatta caccttcgac
     1741 cacaagggtg tgcatttcat cgtggtcgat aacgtctccg acccgggcgc caagattggc
     1801 gacgaacagt tgcaatggct gagcgctgat ttgcagcagc aggacaagaa cgcgcgcatc
     1861 gtggtgttta cgcaccgccc gctgtttgat ctttatccgc aatgggactg ggcgacgcgc
     1921 gacggggcga aggcggttga actgctgatg ccctacaaca acgtcacggt gttctacggc
     1981 cacatccatc tggagaatca ccacatgacc ggacacatcg cgcaccattc ggcgaagtcg
     2041 ctgatcttcc cgctgcccgc tcccggctcg cagcccaagc gcgagccgct gccgtgggac
     2101 gtttcccagc cttaccgcgg tctcggcttc aggagcgtcg aagctgaagt ggacaaagcc
     2161 gattacgcaa tcatcgaata ccccgtactg aaaggatgaa tcgtgaacaa aaatatatta
     2221 tgggtaggaa gcgccatgct tttcctggcc ctcggcagct ctctcggcaa ttacgtgacc
     2281 gcggaaccaa ccgagcgggt aatcaaaatc actgccaagg cgtttgaata tacacctaac
     2341 gaaatcacgc tgaagaaagg cgagccggta gtgctggagc tgacttcgca agacttgttc
     2401 cacggtttca atatcaccga tttgggattg cgcgccgatt tacccccggg catgaccgcg
     2461 cgggtgcggc ttacaccgga caagactggc aacttcgagt ttcactgcga caatttctgc
     2521 ggtaccggtc acgaaaatat gaccggtgtg atcaaggtcg tcgagtaaaa cagattacgc
     2581 ctgtataatg gcagcctttt acgcatagat gagtaatgca ggcgttcctg acctcggcgg
     2641 cgctgattgc cgtcgcagaa attggcgaca agactcagct cctgtcattt gtgctggctg
     2701 cacggctgcg ccgcccgtat cccatcatcg ccggcattct ttccgcaaca ttgtttaacc
     2761 acacgcttgc gggctcgctg ggcgtatggc ttgcgaagct ggttccgcac agctttctca
     2821 actggattgt ggcattgtca tttatcgctt tcggtttgtg gaccttgcac cctgactccc
     2881 tggaggaaga cccgcgcatt cacgaagcgg gcgttttcgt gaccgcgttc atcgcgtttt
     2941 ttctcgccga gatgggggac aaaacgcagc tcgccacgat tgcgctcgcg gcgcgcttcg
     3001 acgctctcgt cgaagtcgtg ctcggcacta cgctgggcat gctgctcgcc gacgtgccgg
     3061 cggtttttat cggtgaaaag ctggcaaaat atattcccat gcggctcgta cgttatgcgg
     3121 ccgctacgct tttcatcgct ttgggccttt ttaccttgat gcggccgctt gagctcgggg
     3181 cctagttttg cactattcgc ggggaagccg gtcttaatca ggtatgcttg acccttaacg
     3241 gttttcaaaa cgggactcat tatgtggctt aaatcgttat tattgttttc gattctgttt
     3301 gctttccttg cgtttcccgc aattgcggca aaccagaccg aggcactcct ggatcaagtc
     3361 atcaacgggg cccaccgtga tccaaaaaat aaagcgcgtg acatttaccg ccatcccaag
     3421 gaaaccctgc tgtttttcgg attgaagccc aacatgacgg tggtggaaat ctggccgggc
     3481 ggcggctggt acaccgaaat cctggcaccg gttttgcgcg accaaggcaa gctttacgcc
     3541 gcgagcttcg ccgtgaacgc caaaaactcg ccggagttcc gcatcgaggt cgagcgggat
     3601 tatctgaaaa aactctcaca acatcccgaa atctacggcc gcatcatcca cacccaattg
     3661 caggcgccgg aatacgtcaa catcgggcca cacggcaaag ccgacatggt gctaactttc
     3721 cgcaacgtgc ataactgggt gaaagacggc aatgccgaag ccactttcaa ggcgttttac
     3781 gatgtattga aacccggcgg catcctcggc gtcatcgatc accgcgccaa gcccggcacc
     3841 cccttgcagg atatgattcg cagcggctat gtgactgaag aactggtgat tggcctagcg
     3901 gaaggcgcgg gcttcaagct ggcggcgaaa tcggaaatca acgccaaccc caaggacacc
     3961 aagaatcatc ccaagggcgt gtggaccttg ccgccgatgc tgcgcttggg cgatcaggac
     4021 aagcaaaaat atctggcgat cggcgagagc gaccgcatga ccctcaagtt cgtcaaaccg
     4081 aaataattca accctttctc tcgcgcagtc cggcgggaat cagccagcgg tcaagcaact
     4141 caaaaccgcc ctgcaccagg agcgccagcg ccgccgcggg gatcgccccg gccagcagcg
     4201 ttgcgttgtc gttcaatgcc agcccccgca caatgcgctc gccgtaaccg ccggcgccga
     4261 caaaggccgc gatggtcgcg gttcccacgt tgatgaccgc cgaagtcttg atgccggcga
     4321 gaatcgcgcg tgaagccagc ggcaactcga tcaagcgcag gcgcggaagc agaggcaatc
     4381 ccagcgattg tgccgcttcc tgcaacgagg gcgcgatatc ggcgagcccg gcataggtgt
     4441 tgcgcacgat cggcagcagc gcgtaaagaa agagtgcgat cagggctggc agcacgccga
     4501 tggtgccgag agccgagatc aaaaatgcga gcagcgccag cgacggcagg gtttgaatca
     4561 ccccgactgc cgaaagaatc ggctgcctgg ctacgggcga gtaagcggcg cagaccccca
     4621 gcgggaggcc cagcagtatc gccccgccga gcgacacgaa caccagcagc aagtgttcag
     4681 cagtcaggtg ccagaaatct gcggcaaaca gcagggtgag aaaatttctc ccggtgctct
     4741 cggtcggttt atgccctgcg ccggatgaga ggaaatccgc agcgatggcc gcaaacggtt
     4801 tgcctgtaag ctccgcctcg gcgttcatcg ccgccatttg cgtttcggaa atccgccctt
     4861 ccagcttttg cagcgccgcc caggttttgg ggaaccggcc gggtacatcg aggcgataaa
     4921 gcaacagcgc gtcataagca gggaaaaaat gcttgccgtc gctcagcacg cgcaggccgt
     4981 atttggcgat tttggcgtct gtggaataaa tgtcaatgac atcgattttg cctttgacca
     5041 gcgcctcata tgccaagccg tggtcgaggc cgagcgggtc aaatggcagt gcgtacgctt
     5101 tttttaacgg cggccagccg tcttggcggt tcaagaattc ctgagacaat cccaatttca
     5161 gttctggctt gcgggcaaga tcgccgatgc tggagatgcc aagctgcctg gcgcgcgcat
     5221 ccggcatggc aagcgcgtag gtgttgttga agcccagcgg cactgccgcc gccaatccca
     5281 aaggcgcaag ctcgcggttc aacgccgcga gatcgcccgc agcgttcttt ttcagcagct
     5341 caaaccagat ggttccagaa tattccggat aaacgtctat gctcccgctt cgcaacgcag
     5401 caaacaatat tccggtattg cctaagccct gtttgtgcac cacttttgct tcattggcat
     5461 tcgccgcggc ccgggcaaga atttcgccga gtacgtacga ttcagtgaaa cgcttggagc
     5521 cgatgttgag cgtggcttcg gcaaaagccg cgggcgcaag caacaacagt gaaaagaata
     5581 atgccttggt cttcatcaag ccgtttctgt aatcaacgag cgttgcgcgt ggatgaattc
     5641 ccttacatac ggctcggcgg ggcgatgcag caaatcattg agggcgccgc gctggatcac
     5701 ccttccttcg cgcaggagca cgatgtcgga ggccaaataa gccgcttcgt tcatgtcgtg
     5761 agtgacgaaa atcaccgtct ttgcgagttg cgcgaatatg cttttgagct cggtttggag
     5821 ctcggagcgg atgatcggat cgagcgcgcc cagcggctca tccagcagca gcgcctcggg
     5881 atcgagcatc agtgcgcgca tcagccccac acgctgccgc tgaccgccgg agagatcgcg
     5941 cggatggcgc gccagagcgg cgggctgcag cctgaccaga tccgccaaat gattcacccg
     6001 ctcgtctatc caggactggg cgcgccgcag gaagcgcgca agcaaagtca cattgtcgcg
     6061 cgccgtcaaa tgagggaata agcctccgtc ctgaatcacg tagccgatgg catggcgcac
     6121 ccgctccgcg ttgtcggcag tcaaaggctc ggtattgaaa actatccggc cgctgtcggg
     6181 ccgaatcagt cccagcatga gccgcagcag cgtggatttg ccgcagccgc tttcgccaat
     6241 cagtgcagtt gtgccggcat gaaacgccag gctggtcggg gcaagagcga tttgccggcc
     6301 ataacttttg caaacatttt cgacagcgat catcccttgc cctttgctgc aaattcgtaa
     6361 gctagcctag catctgtccg tccgccgcgc tactgcgcaa aactgcgcaa ttacaggtaa
     6421 aattgccctg tttaaactcc aatataaagg agaatcacgc atggattcgc aattcttgag
     6481 cttgaaaccc aaaactgaat tcacccgccg cgaatttgtc gtcaccttgc tagccagcgg
     6541 ctttgcgctg gccgtgcagc cggtttccgc gcagaccctg cacactgacg acaagggatt
     6601 gactgcgggc gaagtcaaaa ttccggtaaa cgacggggag attcccgcgt atcgcgcgat
     6661 gcccgccggc ggtggaccgt tcccggtgat tctggtggtg caggaaatct tcggggtgca
     6721 cgagcatatt aaggatgtgt gccggcgctt cgccaagctg ggttatttcg cggtcgcgcc
     6781 cgagctctat tcgcggcaag gcaatgtatc gaaactctcc gatatccagc agattctgag
     6841 cgacgtcgta tccaaagtac cggatgcgca agtcatgtcc gacctcgatg ccaccgtcgc
     6901 ctgggcgaaa aaatccggca aaggcaatac cgccaagctt ggcatcaccg gtttctgctg
     6961 gggcgggcgc atcgtctggc tgtacgcggc gcacaacagc aagctcaaag ccggagtcgc
     7021 ttggtacgga ccactgctgt ggaaaaccaa caaactccag cccaaaaatc cgatcgatat
     7081 ggcgggactg ctcaaggcgc cggtgcttgg cttatacggc ggcgccgacc cgggcattcc
     7141 tctcgatgcc gtggaagctc tgcgcaaagc actgaaagcc gcgggaagca aatcggaaat
     7201 cgtagtttac cccgacacgc ctcacggctt tcacgccgac taccggccaa gctatcgcaa
     7261 ggcgcaggcc gaggacggtt ggaaaaaact gcaggattgg ttcaaaactc acggtgtagc
     7321 ctgagagttg catatgccct gcgaactatg cgaatctgcc ggaggcgagc cgctgtggcg
     7381 cgacgtcctc tgccgcgtgg tgctggtgaa tgatgccgag catcccggtt tctgccgcgt
     7441 catctggaat gcgcacgtca aggaaatgac cgacctgggc gaaaccgaac gccaacatgt
     7501 gctgcgggtg gcgctggcgg tggagacggc attgcgcaag ttgctatcac ctgacaaaat
     7561 caacctggcg agcctgggca acatgacgcc gcacctgcac tggcatgtga ttccgcggtt
     7621 tcacgatgac ccgcattacc ccaacccggt gtgggggcgg aaattgcgtg ccggcccgcg
     7681 cgtcctgccc cgcggcttca gggacactat gcaagcggcg ctaatccggg cgcttggctc
     7741 gagtattaac taatacgcat taacggagca cccaatgctg ccgatgtcgc ttcccgatac
     7801 ccacccatcc gctccctgcg actggaagga tgcggcttcg tgcaagcact ggctggacca
     7861 gttgccgctt accaattttg gccaggcgca gcaggcccta accgctgaac tcaaacgctt
     7921 cctgtgcctg gaaatggcgc cgctcgaacg cctcaacact ctcgagcagc tgcgcgagac
     7981 cgcagcgatg gtgcaaggcg aatgcgccaa gaaatacgcg gccaaaccat tgtcgatggc
     8041 ggccacggag gtcacgcatt ggaaacaatc catagagctc tggcaaaacc tgcgggcggc
     8101 gtatcagcgc tgcttgctgg atgccctggg cggcggtggc gaggcggcgc aacatgccgc
     8161 gctgatttgc cagcgctgtc tgcgctatac cacgctgacc tttttcgaat actacctcac
     8221 caaccaggaa gtagaccctg aattgtggcg ccacttgcat ggactttacg ctttcgccga
     8281 aaaacataat ttaaccgatg aagcggtgcg cgacagcctc atcgaagaag ccaaaaccac
     8341 atcttgcaca gtagcgtacg cgcaggcgct gctgattggg cgaagcaatc cttattcgct
     8401 cacccccaaa caacttgaac tcgcgagccg ctggatcaac aaatggggcg aaaaaatcca
     8461 ggtcaggaca acgcgcagcg aaggcgaaag cgtgctgacg gtggacccgg aaagcaacag
     8521 cggcgccagg caggcgggca aaatccgtga ttcctcttca acacgctact tggtgaccga
     8581 cgatctgggc aaaaaccttg ggataagagt caagctgctg ggagagggaa aatcgcctgc
     8641 agaactgggt ttgggcagcg actgcctaca accgggctgc gaagcgcttt tgaaactcct
     8701 ttatcagcaa tggtgcaagc agggtggcac cgttcgcgcc gcgccgcgcc accaagccga
     8761 ccggaaagcg caactgtgct tcggcattcc ggcggctcac tacttcgtca gtggcaagcc
     8821 gttcaagcag cccggtgcgc cccagctttc ccgccaggag gaagaagaat taaggacgtt
     8881 cggccacatc agcaagcacc gcgaggcgat gcagctttcg caactaggct tcgcgctgga
     8941 ttcctggaaa atcatcaacg actcgtcaat aggcctcggg atgatacgcg atgccaacgg
     9001 cggcggctcg cgtgtgaccc tcaagcaact catcggcgtc tgcccggcgg agagcaagca
     9061 cttcatgctt tgcgtcgtgg agtggattac cctgcagcaa aacggcgaat tgcacattgg
     9121 cgtgcgcgcg cttccgggtc tccccgagcc ggtcgccgtg cgcgccagcg ggacgggaac
     9181 aggagcggct tatgtgcagg cgtttttgct gccggacgtg tcggtgctca atgccgaacc
     9241 gtcactggtg ctccccaacg gctggttcca gaccggccgg attatcgacg tgttcaccgg
     9301 cgaatcacag cggatcaagc tcatcaagct tatggaaaag gggactgact acgaacgggt
     9361 ggatttcggc gctccgtaac gctaaaaacg cacgccggaa gcagtttttg tttttcccca
     9421 actcggttat catgatttcc tgaacgtaac ggcggttcct ttgcgggaaa atcatggctg
     9481 ggctcgacag aaacaccccc accccgaccg aaatcaagct gcaccagcag tcgcgcgtaa
     9541 tggaaataac gttttccgac ggcaagactt tcaagctgcc gtacgaattc ttgcgcgtgt
     9601 attctccttc agccgaagtg cgtggccacg ggccggggca ggaagtgctg caagttggca
     9661 aaaagaacgt ggacattatc ggcatcgagc cggtcggtac ttacgccgtc cagcccagat
     9721 tctctgatgg gcatgaaacc gggatttatt cctgggactt gctttacgac tacggcgtta
     9781 accaggaaaa aatgtggggc acctacctga agcgcctcga agcggctggc gcgagccgcg
     9841 aaataactgc cgctccgttt gggccggggc tgaaatccaa ataggaaaag aacggtatgt
     9901 aaaatattgc caaacctgca tttgctttga ccaaggagga gatatgttga aaacagtggg
     9961 gtgcgcgctg ctgatcggca gcgccatagc gtcggtcaat gctccggcgg aagacgtggt
    10021 gcggttgggc aatcttaagt ttgctcacta tggcgcagtt tcttatatga aggagatcgc
    10081 gcccaagtac ggactcaaga tcgaagaacg catgtttgcg aaaggcatcg acatcctgcc
    10141 ggccatcgtt gccggtgaga tcgatgcgtc ggcgagcgcg ctggatggag cgatagccgg
    10201 gcgcgcgcag ggtgtgccga tttatgccgt ggccggcttc gccaagggcg gtgcgcggat
    10261 cgtcgcccgc agcgacctgg cgatcaaatc agtggccgac ttgaaaggta agaaagtggg
    10321 agtcgcgcgc ggcggtgcgc aggaactgct gctcttcgcc gagctaagca aggcggggct
    10381 cacctggtcg gaaaaacccg ggagtgacgt gcagatcgtt taccttgcct acgccgatct
    10441 caaccaggcg ctgctgggga aggacatcga cgccatgtgc cagtccgaac cgcagtcctc
    10501 ccaagccatc aacaaggggt tcggcataga ggtgatcaag ccttacgaca cgcccatggg
    10561 tgagccggtc cgttctctcg taatcacgga gaagctttac aaggaacgtc cacaggcggc
    10621 gcagaagctg cttaatgtat tcgtcgaggc tacggctttg ttcatcgcca agccggagct
    10681 ggctgagaag tacgtgcgcg aacagatgtt caagaatcag atcacctcgc aggacttcaa
    10741 agacgccatc gccaattcgc cctacaccta cgacctgacg gtcgagcacg tccagatcac
    10801 caccgatctg atgctgaaat atggtgtggg caggctgggt gccaatgctc cggcggcgaa
    10861 ggattgggtg aagctcgacc tgctggaaaa tgccaaggcc gcgctgaagc tcaagtaggg
    10921 gccggtcatg ggagtaactt ctttcagaag tatcctggag ctcgccagag gggtcgtcat
    10981 tcccgttgtg gtcttggcgc tatggcaatt cgtggcgatg agcggctggg tcaacccgca
    11041 gatattgccg gcgccttcgc aggtggtggc caagtggtgg gagtatttgc ggcccacggc
    11101 cccctacagt gcgggcagct acgccttgtg gctggtgtcg ggcgagctgc cgcacgatac
    11161 cttagccagc ctgtcccggg tggtgggcgg gttcctgatc ggcacgctgc tggcgctgcc
    11221 cctgggtctg ctgatgggca ccagcaacag actttacggc ttgttcaatc cgctggtaca
    11281 ggtgctgcga cccattccac cgatcgccta tatccccctc gccatcctct ggtttggtct
    11341 gggcaacccg ccggcctttt tcctgatcag catcggcgct ttctttccgg tgttgatgaa
    11401 caccattgcg ggcgtgcgaa acgtggacgg catttacctg cgcgcggcgc gcaatctggg
    11461 cgccggccag atgaccctgt tccgccgggt gattgtacct gcggcgatgc cgtacattct
    11521 caccggcatg cgtatcggca tcggcacggc gttcatcgtg gtgatcgttt cggagatgat
    11581 cgcggtgaac agcggtctcg gctaccgcat ccttgaagcc cgcgaataca tgtggtcgga
    11641 caagatcatc gcgggcatgt ttaccatcgg cttgctcggg ctgggtatcg acatcttcat
    11701 gaacggcctg aacaactggc tgctcaagtg gcaccgcggc atcgaccaga attaggggac
    11761 agcggcgatg ttcatgcata tcgaaaacgt caacaagcgt tttccggttc ccgggggtga
    11821 agtcgtcgcg ctgaaagata tcaaccttga tgtcggcgag ggcgagtttg tctgtctgct
    11881 ggggccctcg ggctgcggca aatccacctt gctcaatgct atcgccggct tttcaccccc
    11941 gacgagcggt agcatcgcca tcaacagcag ggcggacgcc gccaggtttc cgggccccga
    12001 ccgcggcatg gtgtttcagg aatacgcttt gtttccctgg atgacggtgg cgcagaatat
    12061 tcgtttcggc ctcgaaatca aggggctttc caaagcagaa atgaacggga aagtggacat
    12121 gctcctgcgc aaactcaacc tctacgagtt tcgcgaacgt tttcccaagg acctctccgg
    12181 cggcatgtgc cagcgtgtgg cgattgcccg ggtgctggcg ctcgactcgc cgatcatgct
    12241 gatggatgag ccgttcggtg cgctcgacgc cctgacccgc cgcaccttgc aggacgaatt
    12301 gctgcgcatc tgggacgagt tccggaaaac catcattttc gtcacgcaca gcatcgagga
    12361 gtccatttat ctggccgacc gggtggcggt gatgacgtac cggcccggta ccatcaagcg
    12421 cgacgtgcgc atcacgatgc tgcgtccgcg cgacagcgcc agtgcggaat tcaacgcgct
    12481 gaaaaaggaa ctggccgagt cggtcatgga agaacagatg cgctatactc agactgagat
    12541 ccgcggcagt accgccgagt agcgccggac acggaagcca gcatgcggtt ctccactaca
    12601 cgatgagtaa aaccaccgat ttcggatttc aaaaagtcgc cgaagaggaa aaaacctcgc
    12661 gcgtaaccag cgttttcaat tcggtggccg gccgctatga cctgatgaat gatctcatgt
    12721 cgttgggcgc gcaccggtta tggaagcgct ttgccgttga attaagcgca atcaaagccg
    12781 gtggccgggt tctggatatc gccggcggca ccgccgactt aagcacactg ttcgcaaaac
    12841 gtaccggtga aagcggcgag gtatggctca ccgacatcaa caacagcatg ctctgcatcg
    12901 gccgcgaccg gctgctggat aaaggcctgg ctctgcccgt tgcccagtgc gatgcggaag
    12961 aattgccctt tcccaatgac tatttcgatt gcgtgagcgt cgccttcggc ctgcggaaca
    13021 tgacgcacaa ggaagcggcg ctcaaggaaa tgcagcgtgt tttaagagcg ggtggccgct
    13081 tgctggtact ggaattttcc aaaatctgga agcccctgca accgctctac gatgtttatt
    13141 cattcaaagt gctgcccgct ctgggcaagt tcgtggctaa tgatgccgat agctacaagt
    13201 atcttgccga atccattcgc ttgcacccct cgcaggaaga attgaagcaa atgatggaaa
    13261 gcgccggttt cgaacgcgtc gagtatttca atctcagcgc cggtgttgtc gcgttgcatc
    13321 gtggctacaa gttttaacac atggtttccc caagcggcga accccagcgg gcggaagcgg
    13381 tcttactttt accctatccc tgttccggga tctctcgctc catctctccc ccgggggaga
    13441 aggattggat gagggcggac tcgggcttat ttaatcagaa gttcctaaca cgatctgatt
    13501 cgatatccag ccggaaatcc ctgtaataga atagcaattc gagccgatta agctatgcaa
    13561 gattcaagcc gcaatgaaaa agttcgccct tatactgctt gccgccctgc tgaccgtggg
    13621 gctggcaagc cttgatgctc aggccaaacg tccgggcggc ggcaatgtca ccaagcaaca
    13681 cagcaccgtc acgcataagg ccgaaccgcc cgcccctccc caagcgtccg cgccggcccc
    13741 tgcgcccgcc aaaggcatgc ttgccggtct ggtgtcgggc ggcgtgctcg cctctctgct
    13801 cctgggcgcg gtattcttca ttttccgcat gctgcgcaga aaatcggcgg ctgaatcgct
    13861 gcagtatgtc gccctcggcg cggagaccac accggccatg ccagccagga tttttggaag
    13921 tttcctgcgc ccggcgccct atccggtcgg ttttgaaatc gagccgttcc tgaggaacgc
    13981 caaagcgagt ttcacccgtc tgcaggccgc caacgattcc gcggacctgg acgtgatccg
    14041 cgactacacc acgccgaaaa tgtttgtcgc gcttaaaaaa caaatcgaag cgcgcagcag
    14101 cgacccgcgc aaaaccgaca tcgtcacgct cgatgccgaa gtactagaag tggcgatcga
    14161 agacgatctg gcggttgcta gcgttcgttt cagcggtctg ttgcgggaaa aagattttgg
    14221 cggaccagcg cagccgttct atgaaatctg gaacgtgcaa aagcggctcc acgagcccaa
    14281 gtcggtgtgg ctgcttgcca gaatccagca ggcacaccga atcgttcatg cttgatgcag
    14341 cgattttgaa tcatctgctg cgtggcgagt cctgggcgcg ccaacggctc gaaacccata
    14401 ccggcaaaac cgcgcggttt ttggtgccgc cgttttcatt cacgcttacc gtcgcggaaa
    14461 atggagaagc aaaaccagcc gaggatgtgg caaccgcgga tgtccaattc accatgagtc
    14521 cggtactcct caccaggctt ttcgcccgcg atgaagccgc ctacaaggaa gtgcggatcg
    14581 aaggcgacac cgaatttgcc ggcgccattg catacatcgc caaaaatctc cgctgggacc
    14641 cagcggagga tttgagccgc atcgtcggcg acattgccgc gcagcgcatc gtcaataccg
    14701 cgcatagtat caacaattgg ggaaagcgga gccttgacag ccttgcacgc tcccttgccg
    14761 aatactggac cgaagaacaa ccgctcatcg ctaaaaccgt ggacgtgaaa aaattcgtcg
    14821 tgggagtgga tgccctgcgc gatgatgtgg agcggctgga aaaacgcctg cacaagcttt
    14881 ccaactgacg ctagccgcgc ctgcgtgcgg cggttacaat ccgcgcatgc ggctgtttcg
    14941 ccttctcaaa attatcagca ttagcgtgcg cttcgggctc gacgagtttt tcctcggcca
    15001 cgagcgggtg cgtttgctgc gcgtgctgct caagctccta ctgttctggc gcaggctaga
    15061 gaagccgcgc gcggttcgcc tgcgcctggc gctcgaggcg ctggggccga tcttcgtcaa
    15121 attcggccag gtgctttcca cgcggcgcga tttgctgccc gccgacatcg ccgacgagct
    15181 cgccaagctg caagaccaag ttccgccttt cccctccgct gaagccgtgg ccctgcttga
    15241 gcgggtgtac cggcgaccgc tcagtgacgt gttcagggat ttcgagcgcg aaccggcggc
    15301 aagtgcttcg gttgcgcaag tgcacttagc ggtactgcac gacggcaccg atgtcgcggt
    15361 gaaagtgttg cggccaggaa tcgccgcggt gattgccaag gatctcgcgc tgctcgacat
    15421 cggcgcggga ttgattgaaa ccctctggcg ggacggcaag cgcctcaagc cgcacgaagt
    15481 ggtgagggaa tttgaaaaac acctgcacga tgaactggat ttgatgcgcg aggcggcaaa
    15541 tgccagccag ttgcgccgca attttgaaaa ttcgccgctg ttaatcgtgc ccgaagtgca
    15601 ctgggattac tgcgcaaatg aagtcatggt gatgcagcgc atgcacggca cgccgatcag
    15661 ccaggtgcag cggctgcgag atctcggcgt ggatatcccg aagctcgcgc gagccggcgt
    15721 ggagattttc tt
//
LOCUS       DATIOW010000163         4004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_784237, whole genome shotgun sequence.
ACCESSION   DATIOW010000163 DATIOW010000000
VERSION     DATIOW010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784237"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VLL03_05485"
     CDS             complement(<1..723)
                     /locus_tag="VLL03_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HSS46849.1"
                     /translation="MDIAELLAFVVKNKASDLHLSAGLPPMIRVHGDIRRVNLPAMEH
                     KEVHDMVYDIMNDSQRKFYEENLELDFSFAIPNLARFRVNAFVHNRGAGVAMRTIPSK
                     VSTLEELNAPKTFAAIADQPRGLVLVTGPTGCGKSTTLAAMINYINEKAYGHILTVED
                     PIEFVHESKKCLVNQREVGPHTLSFANALRSSLREDPDYVLVGEMRDLETIRLALTAA
                     ETGHLVFATVHTSSAAKTVDRVI"
     gene            784..1578
                     /locus_tag="VLL03_05490"
     CDS             784..1578
                     /locus_tag="VLL03_05490"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HSS46850.1"
                     /translation="MTAIALALQAVRKRVAASAKAAGRAPREIQLVAISKTFPPSKIV
                     QAHQAGQTAFGENYAQEGVEKILALEKILLEWRVEPTHAGSDRARSDAPRPSYWVASP
                     AGPLLRPLEWHFIGPIQSNKTRLIAEHFTWVHSVDREKIAERLSIARPAHLPPLQVCI
                     QVNVSGEATKSGISPGNAAQLARFIQDLPGLKLRGLMAVPQPIMDFELQRRQLRKLRE
                     MKDALVAQGVPLDTLSMGMSHDLEAAIWEGATLVRVGTAIFGERKP"
     gene            1679..2227
                     /locus_tag="VLL03_05495"
     CDS             1679..2227
                     /locus_tag="VLL03_05495"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSS46851.1"
                     /translation="MKKNLAPLLVLLLALPFAGVEAKTQKPAKLPKLTVTQTIEIKAP
                     PNAVWSRIGDFNGMNTWHPAVAKSEILSGTNNKKGAMRLLTLQDGGTIKEKLLRYNSQ
                     GLSFKYNIIESVLPVSHYVSTIKVKPGKDGGSVVEWYGKFQRKSALAYPPAGEDDATA
                     KNTITAIYKAGLENLKKLAEGS"
     gene            2274..3086
                     /gene="proC"
                     /locus_tag="VLL03_05500"
     CDS             2274..3086
                     /gene="proC"
                     /locus_tag="VLL03_05500"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004550016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HSS46852.1"
                     /translation="MEVTFIGGGNMANAMVGGLLQKGYAAKSLCVVDVSTEARDNINN
                     RFGIAASADLNQDCAEASIFILAVKPQQMREAVRNLKSLLKQQLVISIAAGIRTADLS
                     RWLGGYTQIVRVMPNMPALVLSGFSGLYAMPAVTPAEKRQAEAILGAVGATLWLEREE
                     QLDAITALSGSGPAYVFYFIEALQQAAKELGFDANQARQLALATFSGAVKLASQGEDA
                     QVLRSRVTSKGGTTERAIGKMDEAQIKQHIIDAVRAAAGRSRELGDEFGKAG"
     gene            3207..3779
                     /locus_tag="VLL03_05505"
     CDS             3207..3779
                     /locus_tag="VLL03_05505"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HSS46853.1"
                     /translation="MFGQALQFLLETALDLLTLALLMRFYMQTLRVSFRNPVGEFVVA
                     LTDWLARPARRVIPGLFGFDLASFTLAWAMQVVLLLLLYWIKGFVFAGTPGIAAGILL
                     TLALLEVIKLSVYLLIFLIIVQVLFSWVNPDAPLAPLFNSLARPFLRPFRRLIPPIGN
                     VDLSPLFVLVLAQLALILIAHGYRVIAGMF"
BASE COUNT          873 a         1144 c         1152 g          835 t
ORIGIN      
        1 aatgacgcgg tccaccgttt tggcagcgga gctggtgtgc acggtggcga acaccaagtg
       61 gccggtctcg gcggcggtca gcgccagacg tatggtttcc aggtcgcgca tttcgcccac
      121 cagcacgtaa tccggatcct cgcgcagcga ggaacgcaga gcgttggcga aggaaagcgt
      181 gtgcggcccg acttcgcgct ggttgacgag acatttcttg ctctcatgca cgaattcgat
      241 cggatcttcc accgtcagga tgtgcccgta cgctttttca ttgatgtagt tgatcatcgc
      301 cgcgagcgtg gtggacttgc cgcaaccggt gggacccgtg accagcacca ggccgcgcgg
      361 ctggtcggcg atggcggcga aggttttcgg cgcattgagt tcttctagag tcgacacctt
      421 ggaaggaatg gtccgcatcg ccacgccggc gccgcggtta tgtacgaagg cgttgacacg
      481 gaagcgcgcg agattgggaa tggcaaagga aaagtcgagc tcgaggtttt cttcgtagaa
      541 cttgcgctgc gagtcattca tgatgtcgta caccatgtcg tgtacttcct tgtgctccat
      601 cgccggcaga ttgacgcggc ggatgtcgcc atgcacgcgg atcatcggcg gcagtcccgc
      661 cgaaagatgc aggtcggaag ccttgttctt gacgacgaaa gccagtagtt cggcaatatc
      721 cattataatc cccggggatg tgaaggcagg gagcgctcac tttttctttc aatttaaacc
      781 attatgaccg caatcgcttt ggctttgcaa gcagtcagga aacgcgttgc agcaagcgct
      841 aaagcggccg gtcgggcacc ccgcgaaata caactcgttg cgatcagcaa gacttttcca
      901 cccagcaaaa ttgtccaagc ccatcaagcc gggcagaccg cctttggcga gaactatgca
      961 caggaaggag tggaaaaaat tctggcgctg gaaaaaattt tgctggagtg gcgcgtcgaa
     1021 cccacccacg caggaagcga tcgcgctcga agcgacgcgc ctcgcccctc ctactgggtt
     1081 gcatcccctg cggggcccct gctccgccct ctggagtggc acttcatcgg ccccatccag
     1141 agcaacaaga cgcggctgat tgccgagcac ttcacctggg tgcacagtgt ggaccgtgaa
     1201 aaaatcgcag agcggttatc catcgcgcgc ccggctcatc tgcctccact gcaggtttgc
     1261 atccaggtaa acgtaagcgg ggaagcaacc aaaagcggca tctcgcccgg taatgcggca
     1321 cagttggcca gatttattca agatttgccc gggttgaaac tgcgcggcct catggccgtc
     1381 cctcagccga tcatggattt cgaattgcag cgccggcagc ttagaaagtt gcgcgaaatg
     1441 aaagacgcac tggtggcgca gggggtccca ctggatacac tctcgatggg catgtcgcac
     1501 gacctggaag ccgcgatttg ggaaggcgcc acgctggtgc gcgtcggcac ggctattttc
     1561 ggcgagcgca agccctgaag aacagaccgc aataacgctt gcacaacatc gggctcaacc
     1621 ctatactcga aacgattctt tttcaactct tatctcgacc cttatcggag tcaagcaaat
     1681 gaaaaaaaac cttgcgcccc tacttgttct gcttctggct ttgccgttcg ccggcgtgga
     1741 agcgaaaacc cagaagccag ccaagctgcc aaagctaact gtgacccaaa ccatcgaaat
     1801 caaggccccg cctaatgcgg tatggagcag gatcggcgat ttcaacggga tgaacacctg
     1861 gcaccccgcg gtggcgaaaa gcgaaatcct gagcggcacc aataataaaa aaggtgcgat
     1921 gcgcctcctg accctgcaag acggcggcac catcaaggaa aaactgctgc gctataacag
     1981 tcaaggcctg agcttcaagt acaacattat tgaaagcgtg ctgccggtga gccattacgt
     2041 ttccaccatc aaggtgaagc ccggcaaaga tggcggctcg gtggtcgagt ggtacggcaa
     2101 gtttcagcgc aaatcggcgc tggcctatcc gccggccggg gaggatgacg ccaccgcgaa
     2161 aaacactatt accgcgattt acaaagcggg cttggagaat ctgaagaaac tggccgaagg
     2221 cagttaatgc caattgcggc gccggcgaca ggccggcgct ttacaaaagc tttatggaag
     2281 ttaccttcat cggcggcggc aacatggcaa acgccatggt cggcggacta ttgcaaaagg
     2341 gctacgccgc caagagcctg tgcgtggtgg atgtctccac tgaagcccgc gacaatatca
     2401 acaaccgctt cggcattgcc gcttctgcgg atctgaatca ggactgcgcc gaagccagca
     2461 tcttcattct cgccgtcaaa ccgcagcaaa tgcgcgaagc cgtgcgcaat ctgaaatccc
     2521 tgctcaagca acaattggtc atctcgattg ccgccggcat tcgcactgcc gatctttcgc
     2581 gctggcttgg aggctatacc caaattgtgc gggtgatgcc caatatgccg gcgctggtgc
     2641 tctccggctt ctccggcctt tacgccatgc cggcagtaac gccagcggaa aagcggcagg
     2701 ccgaagcgat tttgggcgcg gtgggcgcaa cattgtggct ggagcgcgag gagcaactgg
     2761 acgcgattac cgcgctgtcg ggcagcggtc cggcttatgt tttttatttc attgaagcgc
     2821 tgcaacaggc agccaaagag ctcggtttcg atgccaatca agcccggcag cttgcgctcg
     2881 ccacgttttc cggcgcggtg aaattggcaa gccagggcga agacgcacaa gtgctgcgaa
     2941 gccgcgtcac atcaaagggc ggcaccaccg agcgcgcgat tggcaagatg gatgaagcgc
     3001 agatcaagca acatatcatc gatgccgtcc gcgctgcagc tgggcgctca cgcgaactcg
     3061 gggatgaatt cggcaaggca ggataataaa tggaaccgag cgggataacc gcgagccgct
     3121 ttccccctca tccaatcctt ctccctcaag ggagaaggac tcctagtccc tctctcccgc
     3181 cgggagagag gtaggagtga gggataatgt ttggccaggc attgcaattt ctcctggaaa
     3241 ccgcgttaga tctgctcacg ctggcgttgc tcatgcgttt ttacatgcaa accctgcgcg
     3301 tctcgttccg caacccggta ggggaattcg tggtggcgct caccgactgg ctagcgcgac
     3361 cggcgcggcg cgtgattccc ggccttttcg gattcgacct cgctagtttc accctcgcct
     3421 gggccatgca ggtcgtatta ctgctcttgc tttactggat taaaggcttc gtttttgccg
     3481 gcactcccgg cattgccgcc ggcattctgc tgaccctcgc cctgctggaa gttatcaagc
     3541 tcagtgttta ccttctgatc ttcctaatca tcgtacaggt gttgttcagc tgggtgaatc
     3601 ccgatgcgcc gctggcgccg ctattcaaca gcttggcccg gccgtttctg cgtccgttcc
     3661 ggcgcttgat tcccccgatt ggaaatgtgg acttgtcgcc gctcttcgtc ctggtgctgg
     3721 cgcagctcgc gttgattctc atcgcccacg gctatcgcgt gatcgcaggc atgttttaaa
     3781 tttctctttt gaaccggatg agagaaggaa tgaaaaaccc ggcactggtt ggagcgcgtt
     3841 ctgttaccca aatagctccc cgagaaggcc aaatctgggc actgggtaac aatgattcga
     3901 attccctaga gtgtccccaa ggtaatgaag tatgccaagg tcgaagcaca tcgatttggt
     3961 tggcatcaat catagcctgt acagccggtc acttgggggg tggc
//
LOCUS       DATIOW010000164         6542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_579687, whole genome shotgun sequence.
ACCESSION   DATIOW010000164 DATIOW010000000
VERSION     DATIOW010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579687"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VLL03_05510"
     CDS             <1..671
                     /locus_tag="VLL03_05510"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HSS46854.1"
                     /translation="QGMLDTYMRLRALLFDPKARDEIARRFAATAMQGDAVSETMRSK
                     LTASTEKVLEMFARDGYAALDAFLKESVPRPEQEQVATIYVKILQSAAVEALQMVRRD
                     AGLKPATINEQTTLFVGDALNAINDSFIYGAPVYLQLADYQEVKASGLQLTRSPGKNI
                     VYTGSVLLVLGVFAMLYLRERRIWMLVKPGNLLFAMSSNRKTLDFEREFERHRQAIAA
                     LIKE"
     gene            673..1818
                     /gene="ccsB"
                     /locus_tag="VLL03_05515"
     CDS             673..1818
                     /gene="ccsB"
                     /locus_tag="VLL03_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HSS46855.1"
                     /translation="MELTQDYPRRTSFFKNLAWFDWTYALLLIAGGVTALRLYGGYMD
                     AYEKGFLLGAVVIFSVLGWHWKPLRTLLMVIAAASLFSIFEYQGSLARASQVFWLKYL
                     FSSQSAIMWMSALFVMATFAYWFALIARSAFAGSVASGLTWSALAMGLSGMLTRWYES
                     YLIGADVGHIPISNLYEVFILFCIITALLYLYYEQRYATKQLGAFVLPVITAAVGFLL
                     WYTFDREAQHIQPLVPALQSYWMKIHVPANFIGYGSFSLAAMVGVAYLLVAAGRLAAY
                     LPQAEVLDDLMYKAIAIGFAFFTIATILGAMWAAEAWGGYWSWDPKETWALIVWLNYA
                     AWLHIRLVKGLRGALLAWWAVAGLFVTLFAFLGVNMFLSGLHSYGTL"
     gene            complement(1744..2403)
                     /locus_tag="VLL03_05520"
     CDS             complement(1744..2403)
                     /locus_tag="VLL03_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006408711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSS46856.1"
                     /translation="MTVLTKQLSALSREALRRHFFALPADDIRLRFGSSMSGEGIDAY
                     VDKVDFDRDAVFGVFDNDLQLIGVAHLALDPEHDAAELGVSVLLGHRGRGVGTALFER
                     AEGHARNHYIKILFMHCLTENQPIMHIAQKSGMRVVTGAGESDAHLELTPPDAANVTD
                     ELMQERVALWDIALKAQFRSAKKIATAMAGKGADELQRAVGMQAAQKHVDAEKSKQGD
                     E"
     gene            complement(2400..3680)
                     /gene="hemL"
                     /locus_tag="VLL03_05525"
     CDS             complement(2400..3680)
                     /gene="hemL"
                     /locus_tag="VLL03_05525"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HSS46857.1"
                     /translation="MTNEELFQRSQQFIPGGVNSPVRAYQSVGGVPRFIARGQGVTVW
                     DAEGKSYIDYVGSWGPLIVGHAHPEVVAAVQQAAERGLSFGAPTAAELEMAELLCRLV
                     PSMEQVRLTSSGTEATMSAIRLARGFTQRSRIIKFVGCYHGHADALLVKAGSGALTFG
                     QPDSAGVPVETAAHTLVLEYNNSAAVEKTMSEFGNQIAAVIVEPVAGNMNLVKPRPEF
                     LQTLRRLCTRHGALLIVDEVMTGFRVGLNGAQGLYGIKPELTTLGKIIGGGMPLGAFG
                     GRRDIMQWLAPLGPVYQAGTLSGNPVAVAAGLATLKLVQAPGFYQKLSGMAERLTAGL
                     SAAAQKHGLAFSAQAAGGMFGIYFRPSPPQSFAEVMQCDQVLFNRFFHAMLDRGVYFA
                     PSAFEAGFVSSAHGGEEITATLAAAEQVFAGLKS"
     gene            complement(3677..4312)
                     /gene="thiE"
                     /locus_tag="VLL03_05530"
     CDS             complement(3677..4312)
                     /gene="thiE"
                     /locus_tag="VLL03_05530"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HSS46858.1"
                     /translation="MVKPGRISGLYAITPDEADTPKLIHQVKLALEGGTRWVQYRNKS
                     ANNRLRQRQAEAIAALCSQFGAALIINDDIELARACNAHGTHLGREDVSLATARSLLG
                     KNKIIGASCYDQIGRAIEAEAQGADYVAFGSFHPSSVKPHTTRPQVQLLAQARTKLSA
                     PIVAIGGITLENAGVLIEAGADAVAVISALFNAPDIKTAAKKFNCLFTSRQ"
     gene            complement(4290..5123)
                     /locus_tag="VLL03_05535"
     CDS             complement(4290..5123)
                     /locus_tag="VLL03_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HSS46859.1"
                     /translation="MSQPPPIVLCFAATDPSGGAGLQADIMTLSSMGCHPLSIVTAIT
                     IQDSAGVEDLLALDPEWVADQARCVLEDMAVSTFKIGLLGSVEITAAIAEVVSDYPDI
                     PLVLDPVLASGRGEELASEEMITAMKELLIPQTTLITPNSMEARRLALEDNNDSDDPG
                     LSECAQRLLDLGCEYVLITGTHENTPQVINTLYAQEGVLRNDSWERLPGSYHGSGCTL
                     AAAVAATLANGLSISEAVRDAQEYTWHALKAAFRPGMGQYIPDRLFWAREEENGETGQ
                     D"
     gene            5172..5336
                     /locus_tag="VLL03_05540"
     CDS             5172..5336
                     /locus_tag="VLL03_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015389172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HSS46860.1"
                     /translation="MKTYMCLICGFIYDEAAGLPEEGIAPGTKWEDLPPNWTCPECGA
                     RKEDFEMVEI"
     gene            5347..5736
                     /gene="gloA"
                     /locus_tag="VLL03_05545"
     CDS             5347..5736
                     /gene="gloA"
                     /locus_tag="VLL03_05545"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HSS46861.1"
                     /translation="MRIMHTMLRVGNLQRSIDFYTQVLDMKVLRRHDYPDGRFTLAFV
                     GYGDEDRTAVLELTHNWDTAQYDLGNAYGHVAIEVPDAYRACEEVKKRGGKVTREAGP
                     MKHGTTVIAFIEDPDGYKIEFIQKKPG"
     gene            complement(5740..>6542)
                     /locus_tag="VLL03_05550"
     CDS             complement(5740..>6542)
                     /locus_tag="VLL03_05550"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HSS46862.1"
                     /translation="CWALRRGQLHRVDDARTGLQCEHVKAAGAPRLHYMCVPMAVHSE
                     TLGLLHLQFGAESSPEEAAQRETAAELLAATFADQIALAIANLRLRDSLRQQSIHDPL
                     TGLHNRRYLEEALTRELARAERKKASLAVIILDVDHFKRFNDTFGHEAGDAVLRSLAQ
                     LIGRQIRGSDVACRFGGEEFVLVLPEADLNIARQRAELLREATQKMQISLGGQTLDVV
                     SISLGLAVFPQHGRKGEELIQAADAALYRAKQAGRNRVMFAEEPPQQA"
BASE COUNT         1251 a         1975 c         1956 g         1360 t
ORIGIN      
        1 aacagggcat gctggacact tacatgcgac tgcgggcgct gttgtttgac cccaaggcga
       61 gggacgaaat cgcgcgccgg ttcgcggcca ccgccatgca gggggacgcg gtgagtgaaa
      121 ctatgcgcag caagctcacc gccagcaccg aaaaagtcct ggagatgttc gcccgcgacg
      181 gctatgccgc gctggatgcg tttctcaagg aatcggtgcc gcggccggag caggagcaag
      241 tcgcaacgat ttatgtcaag attctgcaaa gcgcggcagt cgaggcgctg caaatggtgc
      301 gccgcgacgc gggcttgaag ccggcgacaa tcaacgagca gactacgctg tttgtgggcg
      361 atgccttgaa tgccatcaac gacagcttca tctacggcgc accggtttat ctgcagctcg
      421 cggattacca ggaagtcaag gcgagcggac tgcaattgac ccgctcgcca ggcaagaata
      481 tcgtatatac cggatcggtg ctgctggtgc tgggcgtgtt cgccatgctc tacctgcgcg
      541 aacgccgcat ctggatgttg gtcaaacccg gtaatctgct attcgccatg tccagcaacc
      601 gcaagacgct ggactttgag cgcgaatttg agcgccatcg gcaagcaatc gccgcactca
      661 tcaaggaata aaatggagtt aacccaggac taccccaggc gcacgtcttt cttcaagaac
      721 cttgcctggt tcgactggac ctacgcgctg ctgctcatcg cgggcggcgt gaccgcgctg
      781 cgcctctacg gcggctacat ggatgcctac gagaaaggct tcctgttggg cgccgtagtg
      841 atattcagcg tcctgggctg gcactggaag ccgctgcgca ccctgctcat ggtcatcgcc
      901 gcagcgagcc tgttctcgat ttttgagtac cagggcagcc tcgcccgcgc cagccaggta
      961 ttttggctga aatacctgtt ctccagccag tccgccatca tgtggatgag cgcgttgttc
     1021 gtaatggcga cctttgccta ctggtttgcg ctcatcgccc gttccgcttt tgcgggcagc
     1081 gtggccagcg gcctgacctg gagcgctctg gcgatgggtt tgagcggcat gctcacccgc
     1141 tggtatgaat cgtatctcat cggagccgat gtggggcaca tccccatcag caatctgtac
     1201 gaagtgttca tcctgttttg cattatcacc gcgctgttgt acctgtacta cgagcagcgc
     1261 tacgcgacca agcaactcgg cgctttcgtg ctgccggtga ttaccgcggc ggtggggttt
     1321 cttttatggt acacgtttga tcgcgaagcg cagcacatcc agccgctggt gccggcgctg
     1381 caatcgtact ggatgaaaat ccacgtgccg gcgaatttca tcggctacgg ctcgttctcg
     1441 ctcgccgcga tggtgggcgt ggcttatttg ctggtagcgg caggcagact ggccgcttat
     1501 ctgccgcaag cggaggtgct ggatgacttg atgtacaagg cgattgccat cggcttcgcc
     1561 tttttcacca tcgccaccat tctcggcgcg atgtgggcgg cggaagcctg gggcggctac
     1621 tggtcatggg acccgaagga aacctgggct ttgatcgttt ggctcaatta cgccgcgtgg
     1681 ctgcacatcc gtttggtcaa ggggctgcgc ggagcgctgc tggcttggtg ggcggtggcg
     1741 gggctattcg tcaccctgtt tgcttttctc ggcgtcaaca tgtttctgag cggcctgcat
     1801 tcctacggca cgctgtaatt catccgcgcc ctttccggcc atcgccgtcg cgattttctt
     1861 ggctgaccgg aattgtgctt tcagcgcaat atcccatagc gcaaccctct cctgcatcaa
     1921 ctcatctgtc acgttcgcgg cgtcgggcgg cgtcaattcc agatgcgcgt cggattcgcc
     1981 ggcgcccgtc accactcgca ttcctgattt ttgcgcgatg tgcattatcg gctggttttc
     2041 ggtaaggcag tgcatgaaca agattttgat gtagtgattg cgggcgtgcc cttcagcgcg
     2101 ctcgaacaag gccgtgccca cgccacggcc gcgatgcccg agcaacacgc tcacgcccag
     2161 ttcggcggcg tcgtgctccg gatcgagcgc taaatgcgcg acgccgatga gctgcaggtc
     2221 gttgtcgaat acgccgaata ccgcatcccg gtcgaaatcg accttgtcaa cataagcgtc
     2281 gataccctcc ccgctcatgc tgctgccaaa gcgcaggcgg atatcgtcgg cgggaagcgc
     2341 aaaaaaatgc cggcgcaacg cctcccggct caacgccgac aactgcttgg tcaacacggt
     2401 catgatttaa ggccggcgaa aacctgttcc gctgcggcga gcgtggcggt gatttcctcg
     2461 ccgccatgcg cgctggaaac gaagccggct tcaaacgccg aaggagcaaa ataaacaccc
     2521 cggtcgagca tggcgtggaa gaagcggtta aacaggactt ggtcgcactg catgacttcg
     2581 gcaaagcttt gcggcgggct tgggcggaaa taaatgccga acattccgcc tgcggcctgc
     2641 gctgaaaaag ccagtccgtg tttttgggcg gcggcgctca agccggcggt gagacgttcg
     2701 gccatcccag aaagtttttg gtaaaagccg ggcgcttgaa ccagtttaag cgtggcgagc
     2761 cccgcggcca ccgccaccgg gttgcctgag agtgttcccg cttggtatac cgggcccagg
     2821 ggcgccagcc attgcatgat gtcgcgtctg ccgccaaatg cacccaacgg catgccgccg
     2881 ccgatgattt tcccgagtgt ggtgagttcg ggtttgattc cataaagccc ttgcgcgcca
     2941 ttaaggccca cccgaaatcc ggtcatcact tcgtcgacaa tcagcaacgc gccgtgccgg
     3001 gtgcagaggc gccggagcgt ttgcaggaat tcgggcctgg gcttgaccag attcatgttg
     3061 ccggccaccg gctcgactat caccgcggcg atttgattgc cgaattcgct catggttttt
     3121 tccaccgctg cgctgttgtt atattcaagt accaaagtat gcgcggcagt ctcgacgggt
     3181 acgccggcgg aatcgggctg gccgaaagta agcgcgcccg agccggcttt gaccagaagc
     3241 gcatcggcgt ggccgtgata acagccgacg aacttgataa tgcggcttct ttgagtgaag
     3301 ccgcgcgcga ggcggatggc gctcatcgtc gcttcggtgc cggaactggt gagccgcacc
     3361 tgctccatgg agggtaccaa cctgcacagc agctcggcca tttccagttc ggctgcagtg
     3421 ggggcgccaa agctcaaacc ccgttccgcc gcttgttgca cggcggcgac cacttcagga
     3481 tgggcatggc cgactatcag cggcccccac gagccgacgt agtcgatata gctttttcct
     3541 tccgcgtccc aaactgttac gccctggccg cgtgcgataa aacgcggaac gccgcccacc
     3601 gattggtatg cgcgcaccgg cgaattcacg ccgccaggga tgaattgctg cgaccgctga
     3661 aataattcct cgttggtcat tgccggcttg taaacaagca gttaaatttt ttagccgccg
     3721 tcttgatgtc gggggcgttg aacaaggcgc tgatgaccgc caccgcatcc gctcccgctt
     3781 ctatcaacac gcccgcattt tccaatgtaa ttccgccaat cgccacaatc ggcgccgaaa
     3841 gtttggtcct ggcctgggcc agcaattgca cttgcggccg cgtagtgtgg ggcttgaccg
     3901 aagacggatg gaaactgccg aaggcgacat aatccgctcc ttgcgcttcg gcttcaatgg
     3961 cacgcccgat ctggtcgtag cacgatgcgc cgatgatttt gtttttccca agcaagcttc
     4021 ttgcggtcgc cagcgatacg tcttcccggc ccaggtgcgt gccatgcgca ttgcacgccc
     4081 gagcgagttc gatatcgtca ttgatgatga gcgcggcgcc gaactggctg cacaacgcgg
     4141 cgatcgcttc cgcctgtcgc tgcctgagcc tgttgtttgc gcttttattg cgatactgca
     4201 cccagcgcgt cccgccttcg agcgcgagtt tcacctggtg aatcagcttt ggagtgtcgg
     4261 cctcgtcggg cgtaatcgcg taaagaccgc taatcctgcc cggtttcacc attttcttct
     4321 tcgcgcgccc agaacaggcg gtcgggaatg tattgcccca tgcccgggcg gaacgccgct
     4381 ttcagcgcat gccaggtgta ttcctgcgcg tcgcgcaccg cctcggaaat gctcaaaccg
     4441 ttggcaagcg tcgccgccac cgctgcggcc agagtgcagc cggaaccgtg ataactgccc
     4501 ggcagccgct cccaggaatc gttgcgcaga acgccctcct gcgcgtaaag cgtattgatc
     4561 acctgcggcg tgttttcatg cgtgccggta atcagcacgt attcacagcc caggtccaag
     4621 agccgctgcg cgcattcgga aaggcccggg tcgtcgctgt cgttgttgtc ttccagagcc
     4681 aggcgccgcg cttccatgct gttcggcgta atcagggtag tctgcggaat caaaagttcc
     4741 ttcatcgccg taatcatttc ctcgctggca agctcctcgc cgcgccccga agccagcacc
     4801 ggatcgagca ccagcggaat gtccggatag tccgagacga cctcggcgat tgccgcggtg
     4861 atttccacgc ttccaagcaa accgatcttg aacgtggaca ccgccatgtc ttccagcacg
     4921 cagcgcgcct ggtcggcgac ccactccgga tcgagcgcaa gcaaatcctc cacgcccgcg
     4981 ctatcttgaa tcgtgatggc ggtaacaatg gacaagggat ggcagcccat gctggagagc
     5041 gtcatgatgt cagcctgtaa tcccgcgccg ccggaaggat cggtggcggc gaagcacaac
     5101 acgatgggcg ggggctgaga catgcagggg atttcgttta aaatggcatt ttaacttaaa
     5161 ccgcgagccc catgaaaact tatatgtgcc tgatctgcgg tttcatctat gatgaggccg
     5221 cgggcttgcc tgaagaaggc attgcgccgg ggacaaaatg ggaagacctt ccgccaaact
     5281 ggacctgtcc cgaatgcggc gcgcgcaagg aagattttga gatggtggaa atctgagagg
     5341 accagaatga ggataatgca caccatgctg cgtgtgggta atctgcagcg atcgattgat
     5401 ttttacactc aggtattgga tatgaaagtg ctgcgccggc atgattatcc cgacggccgt
     5461 ttcacgctgg cgtttgtcgg ctatggcgac gaggaccgca ccgcggtgct ggaacttact
     5521 cacaactggg ataccgctca atatgatctc ggcaacgcct acggccatgt cgccatcgaa
     5581 gtgccggacg cctaccgggc gtgcgaggaa gtgaaaaagc gcggcggcaa agtcacgcgc
     5641 gaagcgggac ccatgaagca tggcacgacg gtgatcgctt tcatcgaaga cccggacggc
     5701 tacaaaattg agttcattca gaagaagccc ggctgaggtc tacgcctgct gcggcggctc
     5761 ttcggcaaac atcacccgat tgcgccccgc ctgcttggcg cggtaaagcg cggcatcggc
     5821 cgcctgaatc aattcttcgc ccttgcggcc gtgctgcggg aacaccgcca gtcccagcga
     5881 gatgctgacc acatccagcg tttgcccgcc aaggctgatt tgcatttttt gtgtcgcctc
     5941 gcgcaacagt tccgcgcgct gacgcgcgat gtttaaatcc gcttccggca aaaccagaac
     6001 gaattcctcc ccgccaaaac ggcaggccac gtcgctgccg cggatttgcc gcccgatcag
     6061 ttgcgccagg ctgcgcagca ccgcatcgcc cgcttcatga ccgaaggtgt cgttgaagcg
     6121 cttgaagtga tccacgtcga gaatgatgac cgccagtgag gctttcttgc gttcggcgcg
     6181 agcgagttcg cgggtgagcg cttcttcaag gtagcgccgg ttgtgcagcc cggtgagcgg
     6241 atcgtgaatg gattgctggc gcagtgaatc gcgcaaccgc agattggcga tggcgagcgc
     6301 gatctggtcg gcaaacgtcg cggccagcag ctccgccgcg gtttcccgct gcgccgcctc
     6361 ctcagggctc gattcggcac cgaattgcag atgcaaaagg ccaagggttt cggaatgcac
     6421 ggccatgggc acgcacatgt aatgcagccg gggagcgccc gccgctttca cgtgctcgca
     6481 ctgcaatccg gtgcgcgcat cgtccacccg gtgcaactgc ccgcggcgca gtgcccagca
     6541 tt
//
LOCUS       DATIOW010000165         4854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_72980, whole genome shotgun sequence.
ACCESSION   DATIOW010000165 DATIOW010000000
VERSION     DATIOW010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72980"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1900
                     /locus_tag="VLL03_05555"
     CDS             <1..1900
                     /locus_tag="VLL03_05555"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HSS46863.1"
                     /translation="ELLASPAGALVKPVMAQDPSGELSRLMTSFGGSPEERYGAWFSH
                     DGKRALLIAETKAAGFDVEAQSRAAAAASRAFTAARPLADMHLLLTGPGVFAAESRAI
                     IERDSWRLTLIAAVLVFAILFSVYRSFTVLALGLLPVVSGLLAGIAVVGLVFGMVHGI
                     TLGFAATLIGEAVDYPSYLFTHVAKGERVADTLKRIWPTLRLAVLTTVFGGLTMLLSS
                     FNGLSQLGLLSMTGVLVAGTVTRWILPLLAPEGFAPRQLKPVAGLSWLSASQIAARGV
                     GVVLIAACVILIARREVLWDDDLANLNPIPEAAKRLDKELRNELGAPDVRHLVVVTGR
                     EREEVLQRSETVAQTLHGLAARKFISGFEGAFQYLPSQKKQAERRAALPDNITLRKNL
                     NEALRGLPFREGLFAPFLNDVEAARLAPLMQERDLQGTAFALKVNSLLFRSGGEWVAL
                     LPLRGVEDPAGLAQQFAPIESRDVILLDLKAESNRLINGYRNESLRLTGLGILAITLV
                     LTWGLRQPGRIWRVLKPVLAALVVTLASLLVVGERLNLFHLVSLLLVLGIGLNYALFF
                     NREAGDAEDRQRTHLALIVCSLTTLSAFGCLAFSQTPVLHAIGLTVSLGAVFSLLISA
                     AWARSRIS"
     gene            1945..3126
                     /locus_tag="VLL03_05560"
     CDS             1945..3126
                     /locus_tag="VLL03_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HSS46864.1"
                     /translation="MQPLLVTHFAAANPLGLGAAATLKALREARTGLRPNDFEYAEID
                     TWIGRVDGLERQPVVTELAGFDCRNNRLAQLGLRQDGFEPAVAQARKNYGAERIAVLI
                     GTSTSGILETELAYRKRSPANGTLPADLNYQGSHNLFSVADFVRRYLHLQGPSEVIST
                     ACSSSAKVFASAYRFIEAGWCDAAVVGGVDSLCLTTLHGFASLELLSDQPCRPCDAAR
                     NGISIGEAAGFALLERHGEANIALLGYGESSDAYHMSTPHPQGEGARLSMHKALDCAG
                     LTSEEIDCINLHGTATPANDSVEDQAVCQLFGDNTPCSSTKGWTGHTLGAAGITEAII
                     SILCIEHGFIPGTLNLESPDPQLKSRIVKRNENRTLKRVLSNSFGFGGNNCSLVFGRI
                     A"
     gene            3123..3908
                     /locus_tag="VLL03_05565"
     CDS             3123..3908
                     /locus_tag="VLL03_05565"
                     /inference="COORDINATES: protein motif:HMM:NF025100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HSS46865.1"
                     /translation="MIKVYVQGVSVLAPGLNGWQASRAILAGQAPYRAEPLPRVTPEI
                     LPAVERRRSSDSVRLAVTVAQEAMNQSSLPLNGVATVFASSDGDGQTLHQICEALARP
                     EHDVSPTRFHNSVHNAAAGYWSIATGSKLPSNSLCAFDDSFAIGLLEAATQVVVERVP
                     VLLVAFDLPFPQPLHQVRAVSQAFACAFLLAADPAPAKLSLWEISIENGAAPTALTEI
                     FDGLSSNPAARALPLLQNLARQQDETVRFGYPGRSDVVVKCSR"
     gene            3905..4342
                     /locus_tag="VLL03_05570"
     CDS             3905..4342
                     /locus_tag="VLL03_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxylacyl-ACP dehydratase"
                     /protein_id="HSS46866.1"
                     /translation="MNTGTLSHEAIRTRIPHQGLMCLLDQALNWDETHIICSAVSHRD
                     RNNPLRHGHSLPAVCGIEYAAQAMALHASLTETSSKHGLLAGVRDLVLLAERLDDIPG
                     ELTIRAGKIAAGGEIALYQFSISAGERELLRGRLAAAFTLERS"
     gene            4339..>4854
                     /locus_tag="VLL03_05575"
     CDS             4339..>4854
                     /locus_tag="VLL03_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSS46867.1"
                     /translation="MSPIELSRFAVVIPAYNEAATIRDVASRALRQIPSVIVVDDGST
                     DGTTAALVGLPVTLLSNSENLGKAASLWRGMQHALDTGAGAIITLDGDGQHEPEEIPR
                     LIGEFQRQSDAIIIGSRLGDKHKIPTARYYANRFANFWIAWTAGCPIADSQSGFRIYP
                     ARLLRGSTITHD"
BASE COUNT          889 a         1404 c         1518 g         1043 t
ORIGIN      
        1 cgaactgctt gcatcgcctg ccggtgcgtt ggtcaagccg gtaatggcgc aagatcccag
       61 cggcgagtta tcgcgcttga tgacgtcgtt tggcggcagc ccggaagagc gctacggcgc
      121 ctggttttcg cacgatggaa agcgcgcgct gctgattgcg gaaaccaagg cagcaggctt
      181 tgatgtggag gcgcaaagcc gggctgcggc ggcggcgagc cgcgctttta ctgctgcgcg
      241 ccccttggcg gacatgcatt tgttgttgac ggggccgggc gtattcgccg cggaatcgcg
      301 agccatcatc gagcgcgact cctggcggct gacactgatt gccgcggtgc tggtattcgc
      361 gattttgttc agcgtctacc gttctttcac ggttctggcc ttgggcctgc tgccggtggt
      421 cagcgggctg cttgccggca tcgccgtggt gggtctggtt ttcggcatgg tgcacggcat
      481 cacgctgggt tttgcggcga cgctgatcgg ggaagcggtt gattacccca gttatctatt
      541 tacccacgtg gcgaaaggag agcgggtcgc cgacacttta aagcgcatct ggccgaccct
      601 gcgcctggcg gtgctgacca cggtgttcgg cgggctgact atgctgcttt ccagtttcaa
      661 cgggctgtcg caactgggcc tgctctccat gaccggggtg ttggtcgcgg gcactgtgac
      721 acgctggatc ctgccgctgc ttgcgccgga gggttttgcg ccgcgtcagc ttaaacccgt
      781 ggcagggttg agttggctat cggcttcgca aattgcggca agaggcgtgg gcgtcgtgtt
      841 gattgccgct tgcgtaatcc tcatcgcgcg ccgcgaagtg ttatgggatg acgatcttgc
      901 caatctcaat ccgataccgg aagcggcaaa acggctggac aaggaattgc gcaacgagct
      961 tggcgcgccc gatgtgcggc atctggtcgt ggtgacgggc cgggaacgcg aagaggtttt
     1021 gcaaagaagc gaaacagttg cacaaactct gcatgggttg gcagcgcgga agtttatttc
     1081 cggcttcgag ggcgcgttcc agtacctgcc cagccaaaaa aaacaggctg agcgtcgggc
     1141 ggcgcttccg gacaacataa ccttgcggaa aaacttgaac gaggctttgc gcgggctgcc
     1201 tttccgcgaa ggcttgttcg cgcctttcct taacgacgtg gaagcggcaa ggctggcgcc
     1261 gctgatgcaa gagcgcgact tgcaaggcac ggcctttgcc ttgaaagtga attcgctgct
     1321 tttccggagc ggcggcgagt gggtggcgct gctgccattg cgcggcgtgg aagacccggc
     1381 cgggctcgct caacagtttg caccaatcga aagccgcgac gtaattcttc tcgatttgaa
     1441 agccgaatcc aatcgcctga tcaacggcta ccgcaacgaa tcgctgcgtt tgaccggttt
     1501 aggcatactc gcgatcacgc tggtgctgac ctggggtttg cgccagcctg gtcgaatctg
     1561 gcgggttctt aaacccgtgc ttgccgcgct ggtcgtcact ctggcatcgc tgctggttgt
     1621 gggcgagcgg cttaatctct tccacttggt ttcactgtta ctggtgctgg gcatcgggct
     1681 caattatgca ctgttcttta accgcgaagc gggcgacgcg gaagaccggc agcgcacgca
     1741 tttggcgctt atcgtttgca gccttaccac tttgagcgca tttggctgcc tggcgttctc
     1801 gcaaacgccg gtattgcacg cgatagggtt gacagtgagc ctgggcgcgg tgttttctct
     1861 gctgatctcc gccgcgtggg cgcggagtcg aatttcctag ggcctgttat aattttccaa
     1921 gtttttttcg aggcgggagc gccgttgcag cctttactcg tcacacattt tgccgcggcc
     1981 aatccgctcg gcctgggcgc cgccgccacg ctcaaagcct tgcgtgaagc gcgcaccggc
     2041 ctgcgcccga acgattttga gtacgcggag atcgacacct ggatcggccg cgttgacggt
     2101 ttggaacggc agccggtcgt aacggaactg gccgggtttg attgccgcaa taaccgcctc
     2161 gcccagctcg gtttgcgcca ggatggtttt gaaccggcgg tggcgcaagc ccggaaaaat
     2221 tacggcgcgg agcggatcgc cgtgctaatc ggcaccagca cctcgggcat tctggaaacc
     2281 gagctcgctt accgcaagcg cagccctgcc aatggcacgc ttcccgcaga cctcaattac
     2341 cagggcagcc acaacttgtt ttcggtcgcg gattttgtgc ggcgctattt gcatctgcaa
     2401 gggccatcgg aagtgatttc gaccgcttgt tcctccagcg ccaaagtgtt tgccagcgcc
     2461 tatcgtttta tcgaagcggg gtggtgtgac gcggcggtgg tgggcggagt ggacagtttg
     2521 tgtctcacca ctttgcatgg attcgcttcc ctggagcttt tgtcggacca gccctgccgg
     2581 ccgtgtgacg cggcgcgcaa cggcatctcc atcggcgagg cggccggttt tgcgctccta
     2641 gagcggcacg gggaagcgaa tatcgcgctg ctcggttacg gcgaaagcag cgacgcctac
     2701 catatgtcta ctcctcatcc gcaaggagaa ggcgcgcggt tgtccatgca taaggcgctc
     2761 gattgtgccg gcctgacttc cgaagagatt gactgcatca acctgcacgg caccgcgaca
     2821 ccggccaatg acagcgtcga agaccaagcg gtttgccaac tctttggcga caacacacct
     2881 tgcagttcca ccaagggctg gaccgggcat acgctgggtg cggccggcat taccgaggcg
     2941 attatttcca tactttgtat tgagcatggg ttcattcccg ggacgctcaa tctggaaagc
     3001 ccagacccgc aactcaaaag ccgcatcgta aaacgcaacg agaaccggac gctcaagcgg
     3061 gtactgtcca actcgtttgg cttcggcgga aacaactgca gcctggtttt cggcaggatt
     3121 gcatgatcaa ggtatacgta caaggtgtga gcgttctcgc acccgggctt aacggctggc
     3181 aggcaagccg cgcaatcctt gcgggtcagg cgccttatcg cgcagaaccc ctgccgcgtg
     3241 taacacccga aatcctgcct gcggtggagc gccgccgcag ctcggatagc gtgcgccttg
     3301 ccgtaaccgt cgcgcaggag gcgatgaacc aaagcagcct gccgcttaat ggcgtggcga
     3361 cggtgtttgc ttccagcgac ggtgacgggc agaccctgca tcaaatctgc gaagccctgg
     3421 cgcgacccga gcacgacgtg tcgcctaccc gttttcacaa ttcggtgcat aacgccgcag
     3481 ccggatactg gagcattgcc accggctcca agctgccgtc aaacagcctg tgcgctttcg
     3541 atgattcctt cgcaatcggc ttgctggagg cggcaactca agtcgtggtc gaacgtgttc
     3601 cggtgctgct ggtcgctttt gatttgccat ttccacaacc gcttcatcaa gtgcgggcgg
     3661 tgtcgcaagc ttttgcttgc gcttttttgc tggcggccga tcctgccccg gccaagctca
     3721 gtttatggga aatctccata gagaacggcg ctgcgccgac ggccctgacg gaaatctttg
     3781 acggtttaag cagcaatccg gcggcgcggg cgctgccctt gttgcaaaac ctggcgcggc
     3841 agcaagacga gacggtacgg tttggatacc ccggccgcag cgatgtcgta gtaaagtgca
     3901 gccgatgaat actgggactc tgtcacacga ggcgatccgc acgcgaatcc cgcatcaggg
     3961 cctgatgtgc ctgcttgatc aagcgctgaa ttgggacgaa acgcatatta tttgcagtgc
     4021 cgtgagtcat cgggacagga acaatcccct gcgccatggc cattcgctgc cggccgtatg
     4081 cgggatcgaa tacgctgccc aggcgatggc gctgcacgcc agcctcactg aaaccagcag
     4141 caagcatgga ttgctggctg gcgtgcgcga cctagttctg ctcgcggaga ggcttgatga
     4201 tattccaggc gagttgacta tccgcgccgg gaaaatagcc gcgggagggg agatagcgtt
     4261 gtatcaattc agcatttcgg ctggagagcg tgaattgctg cgcggccgcc tggcagcggc
     4321 atttacttta gaacggtcat gagcccgatt gagctttcgc gttttgccgt ggtgataccg
     4381 gcttacaacg aagccgcaac catccgcgac gtggcaagcc gcgcattgcg gcagattccc
     4441 tccgtcattg tcgtggacga cggctcgact gatggcacca ccgcagctct tgtcgggctg
     4501 ccggtcacgc tgttatccaa cagtgaaaat ttgggcaagg cggccagcct gtggcgggga
     4561 atgcagcacg cgcttgatac aggggccggg gcgatcatca cactggatgg cgacggtcag
     4621 catgagccgg aagagattcc gcgtctgatt ggtgaattcc agcgccaatc cgacgccatc
     4681 atcatcggct cgcgcctggg ggacaagcac aaaattccga ctgcgcgcta ttacgcgaac
     4741 cgttttgcca atttctggat tgcctggacc gccggctgcc cgattgccga cagtcagtcc
     4801 ggcttccgca tttacccggc gcggttgctt cgcggcagca ccattactca tgac
//
LOCUS       DATIOW010000166         4892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1598240, whole genome shotgun sequence.
ACCESSION   DATIOW010000166 DATIOW010000000
VERSION     DATIOW010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598240"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VLL03_05580"
     CDS             <1..418
                     /locus_tag="VLL03_05580"
                     /inference="COORDINATES: protein motif:HMM:NF006452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HSS46868.1"
                     /translation="KKARFGFPEILFNLFPGMGAYSFLKRRVGQKVTEELITSGKISS
                     ADDMLALGVVDVVAEDGQGEAEIAAFIKRQQKTHNGFTALSRVRRRVDQIDYAELLDI
                     VELWVDSALRLNLRDLKLMQRLVSRQNGIGDPQQIH"
     gene            711..2666
                     /locus_tag="VLL03_05585"
     CDS             711..2666
                     /locus_tag="VLL03_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSS46869.1"
                     /translation="MGKGSMMPGNDYTIQEVSAKLGIPIQKLRRWDAQGVLVAHRSHG
                     GHRRYSRELIDRLATASTVAQLSEKQSVELAAVKKTLREKHRIIQLLLDSESRYRDLV
                     ETSHDLIWATNALGRFTYLNNAAMEIFGLNPEALLGRCFFDFEARPSQVSNRRFLATL
                     KRHNEIKNYITHLITASGDDRWIGINARLSHDDEGRIIGIRGTARDITEQHLAALRIQ
                     HMSQHDMLTDLPNRACLQQTLEAAIQSGQVGALLFLDIDNFKYVNDNFGHHAGDQLIG
                     GISGVLREVMNEFDGQLFRIGGDEFAIHLPAALRKEAIGVAEKGLESVRYYRFVHGGK
                     QHVTNMTASIGIALYPFHGNDVASLLSNVDIAMYQAKDHGRDGYALFDQDAKSIRSTH
                     KRVHWAKKLRDALDEDRLLLYSQPVVRLADRKLMHQEILLRMQDEQGKIVLPGDFIEI
                     AESIGMIQEIDMLVVKKLIHYLQTHPETEQKRRYFVNLSRASISDPRWVRRFQHMLGA
                     NDIAPSQLVFEITETAAMSEIDVTVSFIRELKEMGCRFALDDFGSGFSSFYYVKKFDV
                     DYLKIDGNFVRDLVHDESNRIFVKALNDVAKGLHKQVIAEWVENKEVLDALIEMGTQY
                     GQGHLFQHPLPLIQPPASELDQQPSVA"
     gene            complement(2694..3263)
                     /locus_tag="VLL03_05590"
     CDS             complement(2694..3263)
                     /locus_tag="VLL03_05590"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HSS46870.1"
                     /translation="MKKTYPKQEPDFRETGLSSKMAHRGSFFELKEDRVRLPDGKEVV
                     REYLIHPGAVVIIPVLDNGDLVMEWQYRYALRRHFYELPAGKLDPGEDPLQCAKRELL
                     EETGYVAQRWQCLGVAYPCVGYSNEKQLFYLAQGLSQRERQLDDGEFLEVLTLDLATL
                     LAWVKEGKITDGKTLNGLFWFDRLRRGAI"
     gene            complement(3260..3547)
                     /locus_tag="VLL03_05595"
     CDS             complement(3260..3547)
                     /locus_tag="VLL03_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HSS46871.1"
                     /translation="MVFLLLLAFIAANLPFFSERLLFVIRPESGVKNFGWRLLEVLLL
                     YLIVGGVGYLLEARAGNVHEQGWEFYAITLCLFLVFACPGFVYRYLWRKQP"
     gene            complement(3556..>4892)
                     /gene="nuoN"
                     /locus_tag="VLL03_05600"
     CDS             complement(3556..>4892)
                     /gene="nuoN"
                     /locus_tag="VLL03_05600"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HSS46872.1"
                     /translation="ITYHLTQLTLLGCAVVTVAVSEPGIRYSFSRMFVADNMADVLKI
                     MVNISVCVMLIYSRNYIYLRGMFRGEFFALVLFAMLGMMVMISANHFLTLYLGLELLS
                     LSLYAVVALQRDSAMATEAAMKYFVLGALASGLLLYGMSMLYGATGSLEITQVAKAIH
                     AGVPQKTVLVFGLVFVVAGLGFKLGAVPFHMWIPDVYHGAPTAITLFIGSAPKLAAFA
                     FAMRLLAGSLQGLVVDWQGMLIILAVLSMAVGNVTAIAQSNLKRMLAYSTISHMGFML
                     LGILSGSLNGYSSAMFYMLVYVLMSLGSFGMILLLSREGFEAENLDDFKGLNARNPWY
                     ALMMLLLMFSMAGVPPTVGFFAKLSVFEAALEAGYVWLVVMAALFTLIGAFYYLRIVK
                     LMYFDDPVDLAPILPQPDARLLISANGLAMLVLGVLPHPLMALCVNSIARSL"
BASE COUNT         1165 a         1476 c         1361 g          890 t
ORIGIN      
        1 gaaaaaagcg cgcttcggct tcccggaaat cctgttcaac ctgttccccg gcatgggagc
       61 ctattccttc ctcaagcggc gggtcggcca gaaagtgacc gaggaactca tcaccagtgg
      121 taaaatctcc agcgctgatg atatgctggc gctgggtgtg gtagatgttg ttgcggagga
      181 cggccagggc gaagccgaaa tcgcggcgtt catcaagcgc cagcagaaaa cccacaatgg
      241 tttcaccgcc ttgtcccggg tacggcggcg ggtggaccag atcgactacg ccgagctgct
      301 ggatatcgtc gagctgtggg tggatagtgc gttgcggctt aacctgcgtg atcttaagtt
      361 gatgcagcgc ctggtgtcgc ggcaaaatgg catcggcgac ccgcaacaga ttcattaagt
      421 accgtcacgc cagcgggaaa cgccttcccg caggcgtgga accttctgaa taagtcctcc
      481 gcgaattgcg agttgcaggt aaaatacgga tgggtgcaag gcacgcgacg aaggtcgtag
      541 cgaagactac gaaaccgaga agggcccgcg cagcgttcgc acctgcgaag ctgtgcagcg
      601 gagcgggcca gcgctcgcct tcgcggtcgc acgtacccga ggctccacgc tccggcgggc
      661 ctcccggaaa accgccgtca caaccgcgtg ggatttaatc agaggttcct atgggcaaag
      721 ggagcatgat gccggggaat gactacacca ttcaggaagt ctcagccaag ctgggcatcc
      781 cgattcagaa gctgcgccgc tgggatgcgc agggggtact ggtcgcgcac cgcagccatg
      841 gggggcaccg gcgttattca cgcgagctga tcgaccgcct cgccaccgct tcgacggtcg
      901 cccagctttc cgaaaagcaa tcggttgaac tcgcggcggt gaagaaaacg ctgcgcgaaa
      961 agcaccgcat catccagttg ttgctggata gcgaaagtcg ttaccgcgac ctggtggaaa
     1021 cctcgcacga cctgatttgg gcgaccaacg ccttgggccg cttcacctac ctcaacaacg
     1081 ccgccatgga gattttcggg ctcaatcccg aagcgctgct gggccgctgc ttcttcgatt
     1141 tcgaggcacg gccgtcgcag gtttccaacc ggcgcttcct tgccacgctt aagcgccaca
     1201 acgagatcaa gaactacatc acccacctga ttaccgcttc gggtgacgat cgctggatcg
     1261 gcatcaatgc ccggctgtct catgacgacg agggacgcat catcggtatt cgcggcacgg
     1321 cgcgcgacat caccgagcag catctcgccg cgctgcgtat tcagcacatg tcgcaacatg
     1381 acatgctcac cgatcttccc aaccgcgcct gcctgcagca aacgctggaa gccgcgatcc
     1441 aatcgggcca agtcggcgcg ctcttgtttc tcgacatcga caacttcaaa tatgtgaacg
     1501 acaacttcgg gcaccacgcc ggcgaccagt tgattggcgg catcagcgga gtgttgcgcg
     1561 aagtaatgaa cgagtttgat ggccaattgt ttcgcatcgg cggcgatgag tttgcgattc
     1621 acttgcccgc cgccttgcgc aaggaagcca tcggcgtggc ggaaaaaggg ctggaatccg
     1681 tgcgttacta ccgtttcgtg catggtggca agcaacatgt taccaacatg accgcttcga
     1741 tcggcattgc gctctatccg ttccacggca acgatgtcgc cagtctgctt tccaacgtgg
     1801 atatcgccat gtaccaggcc aaggaccacg gccgcgacgg ctacgccctg ttcgaccagg
     1861 acgcaaagag catacgcagc acgcacaagc gcgtgcactg ggcgaagaaa ctgcgcgatg
     1921 cgctggacga agaccgtctg ttgctctatt cccagccggt ggtgcgcctc gccgaccgga
     1981 aactcatgca ccaggaaatc ctgctgcgaa tgcaagacga gcagggcaaa atcgtcctcc
     2041 ccggcgactt cattgaaatc gcggaatcta tcgggatgat ccaggaaata gacatgctgg
     2101 tggtgaaaaa actcattcac tacctgcaaa ctcatcccga aacggaacaa aagcggcgtt
     2161 atttcgtcaa cctctcccgc gccagtattt ccgatccgcg ctgggtcagg cgtttccagc
     2221 atatgctcgg ggccaacgac attgccccca gccagctagt gttcgagatc accgaaaccg
     2281 cggcgatgtc ggaaattgac gtcacggtgt ccttcatccg cgagctcaaa gaaatgggct
     2341 gccgcttcgc gctggatgat ttcggctcgg gcttcagctc cttctactac gtcaagaaat
     2401 tcgacgtgga ttacctgaag atagacggca atttcgtgcg cgacctggtt cacgatgaat
     2461 ccaaccgcat tttcgtgaaa gcgctgaacg acgtggccaa gggcctacac aaacaggtca
     2521 tcgccgaatg ggtggaaaac aaggaagtgc tggacgcctt gattgaaatg ggcacgcaat
     2581 acgggcaagg ccatttgttc cagcaccccc tgccactgat tcagccacct gcctccgagc
     2641 tggatcagca acctagcgtc gcctagcccg gccgcctact caaaagagta gtgttatatc
     2701 gctcctctgc gcaaccgatc aaaccagaac aagccgttga gagtcttgcc atcggtgatc
     2761 ttgccctctt tcacccaggc cagcagagtc gccaaatcca gcgtcagtac ttccagaaac
     2821 tccccatcat cgagttgacg ttcccgctgg ctcaagccct gggcaaggta aaataactgc
     2881 ttctcgttgg aatagccgac gcagggatag gcgaccccga ggcattgcca gcgttgcgcg
     2941 acataacccg tctcttccag cagctcgcgc ttggcgcatt gcagcgggtc ttcaccggga
     3001 tcgagcttgc ctgcaggcaa ctcgtaaaaa tgccgccgca gtgcatagcg gtattgccac
     3061 tccatcacca gatcgccgtt atccagcacc ggaatgatca ccacggcgcc ggggtggatc
     3121 aggtactcgc gcaccacttc cttcccgtcc ggcaagcgca cccggtcttc cttcagttcg
     3181 aaaaagctgc cgcgatgagc cattttcgat gaaagtccgg tttctctgaa atcgggttcc
     3241 tgcttcgggt aagtcttttt catggttgtt ttcgccacaa ataacgataa acgaaaccgg
     3301 ggcaagcaaa aaccaggaac aggcacaggg tgatggcgta aaactcccag ccttgttcat
     3361 gcacattgcc ggccctggct tccagaagat aacccacgcc accgacaatc aaatacagca
     3421 ataaaacttc gagcagccgc cagccgaaat tctttacgcc ggattcgggg cggatgacaa
     3481 acaggagacg ctcgctgaaa aaaggcaggt tggcggcgat gaaagccagg agcaggagaa
     3541 aaaccatgcc tgcattcaca gcgagcgggc aatcgaattc acgcacaacg ccatgagcgg
     3601 gtgcggcagc acgccgagca ccagcatggc gagcccgttg gcgctgatga gcaagcgcgc
     3661 gtccggctgc ggcaggatcg gcgccagatc caccggatcg tcgaaataca tgagcttgac
     3721 gatacgcagg taataaaacg cgccaatcaa ggtaaacagc gccgccatca ccactagcca
     3781 tacatagccc gcttccagcg ccgcttcgaa caccgacagc ttggcgaaga aaccgacggt
     3841 cggcggcaca ccggccatgg agaacatcag gagcagcatc atcaatgcat accagggatt
     3901 gcgcgcattc agtcccttga agtcgtcgag gttttccgcc tcgaagcctt cgcgggagag
     3961 cagcagaatc atgccgaaag agcccaggct catcagcaca tagaccagca tgtagaacat
     4021 cgcggaactg tagccgttga ggctgccgct taaaatcccc agcagcatga agcccatgtg
     4081 cgaaatggtg gaataggcaa gcatgcgctt gagattactt tgcgcaatcg ccgtcacgtt
     4141 gccaaccgcc atcgacagca cggcaaggat aatcagcatc ccctgccagt ccacgaccag
     4201 tccctgcagg ctgcccgcca aaagacgcat cgcgaaagca aacgcagcga gcttgggcgc
     4261 ggaaccgatg aaaagcgtga tcgcagtcgg cgcgccgtga tagacgtcgg gtatccacat
     4321 gtggaatggc acggcgccca gcttgaagcc cagaccggcg acgacgaaca ccagcccgaa
     4381 caccagcacc gtcttctgcg gcacgcccgc atgtatcgct ttcgccacct gggtgatttc
     4441 cagcgagccg gtcgcgccgt aaagcatgga catgccgtag agcagtaggc cggaagcgag
     4501 cgcgcccagc acgaaatatt tcatcgccgc ctcggtcgcc atggccgaat cgcgctgcag
     4561 cgccaccacc gcgtacaggc tgagcgacaa cagctccagc cccagataca gggtgaggaa
     4621 atggttggcg gaaatcatca ccatcatccc cagcatggca aacaggacca gcgcgaaaaa
     4681 ctcgccgcgg aacatgccgc gcaaatatat gtagttgcgc gaatagataa gcattacaca
     4741 caccgagata ttcaccatga tcttgagcac gtcggccata ttgtcggcga caaacatgcg
     4801 gctgaagctg taacggatcc ccggttcgct tacggccacc gtgaccacag cgcagcccaa
     4861 cagagtgagc tgcgtcaaat gataagtgat ga
//
LOCUS       DATIOW010000167         2528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1190850, whole genome shotgun sequence.
ACCESSION   DATIOW010000167 DATIOW010000000
VERSION     DATIOW010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190850"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="VLL03_05605"
     CDS             <1..175
                     /locus_tag="VLL03_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HSS46873.1"
                     /translation="PILGIACPNILFPYAREVISDVVSRAGFPPVILNPVNFEALYQH
                     RRQQPQPSAETAH"
     gene            189..626
                     /locus_tag="VLL03_05610"
     CDS             189..626
                     /locus_tag="VLL03_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HSS46874.1"
                     /translation="MHRCLLTFFLMLVAAAAGAAEFRSVAENATVLYDAPSLKAQKLY
                     LLGRDYPLEVVVALETWIKVRDASGELAWVEKKSLSDKRSVMVNVPLAEVRQAPNDSA
                     PLVFQAEKSVMFELVEFSNPGWIKVRHRDGQTGYVHSNQVWGV"
     gene            623..1600
                     /locus_tag="VLL03_05615"
     CDS             623..1600
                     /locus_tag="VLL03_05615"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HSS46875.1"
                     /translation="MKLAILGAGAWGTALAITLSSRHNIGLWEHDTGHFAHLKAHHAS
                     RFLPKFSLPETIEFHSSVSAALAQAGLALVAVPTLGLRQTLREMKLSRVPAVLACKGF
                     EPGSQYLPHQVAEQELAPGTSCAILSGPSFAQEVARGLPTALTLASTDKAFAQAAAKE
                     LHSQKLRIYSSDDMIGVAIGGAMKNVMAIAAGIADGLGLGNNARAALIARGLAEITRL
                     GLKLGGRVETFMGLAGAGDLILTCTGDLSRNRTVGLMLAEGKSLPKILAELGHVAEGV
                     TSAREVQRLAGEMKVEIPITRAVNQVLYENVPAKAALQELLNRDPKAEF"
     gene            complement(1657..2121)
                     /gene="trmL"
                     /locus_tag="VLL03_05620"
     CDS             complement(1657..2121)
                     /gene="trmL"
                     /locus_tag="VLL03_05620"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HSS46876.1"
                     /translation="MFNLVLYQPEIPPNTGNIIRLCANTGVSLHLVKPLGFRLNDRQL
                     QRAGLDYHELANIAVHESWESCKEHFSGRRLFAITTRGEQRYDKVTFNPGNVIVFGPE
                     TRGLPRFLLEGFPDQNRIRLPMLPQNRSLNLSNAAAVVIYEAWRQAGFKGGK"
     gene            2339..>2528
                     /locus_tag="VLL03_05625"
     CDS             2339..>2528
                     /locus_tag="VLL03_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46877.1"
                     /translation="MFLAAACAPSRSTLRESESGAPGHRALSLVAYWATTQPALLASC
                     PAAGCGFDQEILPAGIAAV"
BASE COUNT          508 a          714 c          765 g          541 t
ORIGIN      
        1 accgatactg ggaattgcct gccccaatat tcttttcccc tatgcgcgcg aagtgatttc
       61 cgacgtagtg agccgtgctg gttttccgcc ggtgatcttg aacccggtga atttcgaggc
      121 attgtaccag caccgccggc aacagccaca gcccagcgcg gaaaccgcgc attgagcttg
      181 ttttagccat gcatcgttgt ctgctcacgt ttttcctcat gctggttgcc gcagcggcgg
      241 gcgctgcgga attccgttcc gttgctgaaa acgccacagt gttgtatgac gcaccttcac
      301 tcaaagccca aaaactttac ctgctgggcc gcgattatcc gttggaagtc gtggtggcgc
      361 tggagacctg gatcaaggtg cgcgatgcca gtggagagct tgcttgggtg gaaaaaaaat
      421 ccttgagcga taagcgcagc gtaatggtca atgtgccgct tgccgaggtg cgtcaggcgc
      481 ccaacgacag tgccccgctg gtgtttcagg cggagaagtc ggtgatgttc gaactcgtgg
      541 aattctccaa cccgggctgg atcaaggtgc ggcaccgcga cgggcaaacg ggttatgtac
      601 acagcaatca ggtgtggggt gtatgaagct cgcgattctg ggcgcaggcg cctggggcac
      661 ggcgctggcc attaccctgt cctcgcgcca caacatcggc ttgtgggaac atgataccgg
      721 gcattttgcg cacctaaagg ctcaccacgc ctcgcgcttc ctgccgaaat tttccctgcc
      781 ggaaacgatt gaatttcatt cgtctgtaag cgccgcactg gcgcaggccg ggctggcgct
      841 ggttgccgtg ccgactctgg gcttgcggca gactttgcgt gagatgaagt tgagccgtgt
      901 gccggcggtt ttggcctgca agggtttcga gcccggcagt cagtatctgc cgcatcaggt
      961 cgccgagcag gaacttgcgc cgggcacctc ctgtgcaatt ttgtcgggac ccagcttcgc
     1021 ccaggaagtg gcgcgaggcc tgcctacggc gctcaccctt gcttccacag acaaggcgtt
     1081 tgcgcaggcc gcagcaaaag aacttcacag ccagaaactg cgcatttact ccagcgatga
     1141 catgattggc gtcgcgatcg gcggcgccat gaaaaacgtg atggcgatcg ccgccggcat
     1201 cgccgacggt ctgggcctgg gcaataacgc gagagcggct ttgattgcac gcgggctcgc
     1261 tgagatcacc cggctgggac tgaaactggg tgggagagtg gaaactttca tgggactcgc
     1321 aggcgccggt gatttgatcc tgacatgcac cggtgatctt tcaagaaacc gcaccgtggg
     1381 cttaatgctg gccgagggaa aatccttgcc aaaaattctt gccgagctcg gtcacgtggc
     1441 ggagggagtg accagcgcgc gcgaggtaca gcggcttgcc ggtgaaatga aagttgagat
     1501 tcccattact cgcgcagtta accaggtgct atacgaaaac gttccggcaa aagccgcgct
     1561 gcaagagctg ctcaatcgtg atccgaaagc tgaattttag cggtcagctt tggatgcggt
     1621 tggttttcac tgagagctga tggctaaata gctgttttat tttccacctt taaaacctgc
     1681 ctgccgccac gcttcgtaaa ttaccactgc agctgcattc gacagattca ggctgcggtt
     1741 ctgcggcagc atcggcagcc ggatgcggtt ttgatcagga aatccttcca gcaggaatcg
     1801 cggcagtccc cgcgtttccg ggccgaaaac gattacattg ccgggattga acgtaacctt
     1861 gtcgtaacgc tgctcgccgc gggtggtgat ggcaaacagg cggcggccgg aaaaatgctc
     1921 tttgcagctt tcccagcttt catgcactgc gatattggct aattcatggt aatccagtcc
     1981 tgcacgctgc aattgtcggt cattcagcct gaaccccaga ggtttcacca gatgcaggga
     2041 cacccccgta tttgcgcaca ggcggatgat atttccggta ttcgggggga tttcaggctg
     2101 atacaggacc aaattaaaca tagggaacct ctaattaagt cctccgcgtg acgcgttgcg
     2161 ggcgagaaac ggtcggatgc aaggcgcaag ggcgaagcgc tacctagtag taacgcaagc
     2221 ccgagcaacg ctgcaggcgg ccgtacatcc gccgcaaccc gcatgggacg ggggctgccg
     2281 cgacacggta agcgaagctt ggggtgcggg agtggcagca tgagcgcccc gtttcacaat
     2341 gtttcttgcg gcagcttgcg ccccttcccg ctccacgctg cgcgagtccg agtccggggc
     2401 gcccggccac cgggctttgt cgctcgtcgc ttattgggca accacccaac cggcgctcct
     2461 cgcttcgtgc ccggcagccg gatgcggttt tgatcaggaa atccttccag caggaatcgc
     2521 ggcagtcc
//
LOCUS       DATIOW010000168         6909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1293725, whole genome shotgun sequence.
ACCESSION   DATIOW010000168 DATIOW010000000
VERSION     DATIOW010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6909
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293725"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VLL03_05630"
     CDS             <1..230
                     /locus_tag="VLL03_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSS46878.1"
                     /translation="KPDLVLMDVVMPGQNGFQATRALNKDEETKNIPVIIVTSKSQET
                     DKIWGMRQGAKDYIVKPVDQAKLLAKIRALG"
     gene            231..746
                     /locus_tag="VLL03_05635"
     CDS             231..746
                     /locus_tag="VLL03_05635"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HSS46879.1"
                     /translation="MAKHFSLREFQQGLSARLQALADTPSAAASKLGVQAGRDLWLVD
                     LIEAGEVVPLPELIKVPLTRSWFSGVANIRGVLYSIVDFSAFMGGEPTALGPDTRVLL
                     VGQKLAANCGILVDRMMGLRRIEQLQRVKSAAPAAPWANAEYTDGEDRRWKELQLRKL
                     VEHPDFLQIGV"
     gene            808..2937
                     /locus_tag="VLL03_05640"
     CDS             808..2937
                     /locus_tag="VLL03_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HSS46880.1"
                     /translation="MALLKSKVSEKAKARDTKDPSFDPMHTTSIMDTAKAQAVPWALP
                     LIGNMPINRQYQILGALVGLCLVAAALFFFASVRETSNNAVYLTTATEMQMLSQRLAK
                     GAQQAVLGNVSGFEQLRQGRDKFGADLNNLINGSANVPAATKLAQSNLDEISQLWAPM
                     EQKASLILSQKTDLVNLGKNVLAINNNNPRLLELAQEVESTLTTNNAPVRAVRVASRQ
                     VMLTQKLAKNANALLSGELVDPEVAFLLAKDTNTFRDNLLALINGSEQLHIAPAKDQE
                     SLERLKDLQDAFKEFEGSVTSILQNQQKLIQAKQAGQSIFVDSEKLLASLQKLTDRFR
                     AEAEKQGMLIAAILFAALMLIFLALMGFASVFDARRRALESERENKRNQKAILQLLND
                     MGDLAQGDLTVHAQVTAEITGAIADSINFTVDELRTLVTGINKATGQVTSATEQAKVI
                     STVLLEAAQKQAQEIKDTTVSVLEMARSMNEVSANANQSAKVARQSLNAAEKGTAAVQ
                     NSISGMNEIREQIQETSKRIKRLGESSQEIGEIVELISDITEQTNVLALNAAIQAAAA
                     GEAGRGFTVVAEEVQRLAERSAEATKQIGAIVKTIQTDTHDAVAAMEKSTQGVVEGAE
                     LSDAAGQALSEISDVSRSLAQLAATISSATQSQAESANRVAKNMQDILSITDQTTEGT
                     KRTAVSIGQLAQLAAELKSSVSGFKIS"
     gene            2944..>6909
                     /locus_tag="VLL03_05645"
     CDS             2944..>6909
                     /locus_tag="VLL03_05645"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HSS46881.1"
                     /translation="MSAQREFDIGPLTWVKSEIDQALSRARQSLDQAAGGDAAPLKFC
                     QTHLHQAGGAVQMVGLDGVARFSEEAERLLGLLGNHPDKITSATLELVKRALNAMAQH
                     LDGLIDGKADVPLNLFPIFKELAQAAGTENISESELFFPDLSLRPPRHPQASAVAEAD
                     MGNFIKKQRGEFQRGLLDWLRNPDDSQGVKRMHETLRAIEQSQPLPAQRTFWWAANGF
                     IEALVHKEVEADLHAKQLCAHIDLQIRRLAAGSYKVAERQLREVLYFVARCQPVTERI
                     REIQELYALGSLLPDKAAVPGLMEYDASKLAPVLRDFREALTAAKDAWAKYTSGARDA
                     LPRFREQVTKLIELAVELGAQPLQKLLEVIKIALARAPVKGEENEVLALEMATALLLA
                     ESVAENLADPADELPRQIEVMASRLLAALGEPSQGGELPDVPLLDEITRKAQEKLLIA
                     QVVNEIQSNMSHIEQVLDSFFRDAAKRNQLPALAPFLKQIEGALNILGLDLAVKLLAE
                     CQRFIQKFSDADYTAPAQELELLAEGLSSLGFYINEVENPDREQIITPVLRKFLDESG
                     SAAALEEAAPETTTMLTIESSLERQKRAAQNLFASWQEKPGDAVTREKLQDNLEAIRQ
                     DADLAKNKHLQRQAAGALELLKFDAVAEDAKLAEMMMTLSSTQVRPEAPSEHTQRMLA
                     ASEEVVDQELLQIFLNEAQDVLTTINEHLGRVTGEPHNTDYLTTIRRGFHTLKGSGRM
                     VGLKDLGEVAWSIEQVMNKWLQEERSATPQLLYLIKLAHAAFSSWVSTLQARGQAEIN
                     APNIIALAEALLHGMEAEAAAVAAAAAEVTELAAASAPETVPAKSAVEESIVIGATSL
                     EPVLYQTFVDEAADHIASLKDECARLQQNPEQSPSEALMRASHTLSGISHTAGFTDLA
                     ELSSALEQWAMELIEHPAGMSEMELNATVQAVAKLEQMVHAVRERRVPESAEKETQEL
                     QLLLSAAIIQTKPMVSTRLIQAETPAARAPDEPAPVAETKSAASAAEASPIEELGWTR
                     ERRAIRDDLDAQLLPIFLEEAHDLVPIIGEDLRSWRTSPEDKKSPMILARALHTLKGS
                     ARMAGAMRLGELTHNMESKVENAAQLGQPMMSLFDDLENQFDRMVAEIEKIKSGAQPE
                     AAPVAEAAAAEAAPTEATEPEPIQVRAMLRVRADIVDRLVNEAGEVSIARARIEGEMR
                     DFKQSLLELTESVARLRHQLREVEIQAETQMQSRMSQMGEEAQRFDPLEFDRFTRLQE
                     LTRLMAESVHDVVTVQQTLLKNVDETDAAILQQARLNRDLQDELMRVRTMPFSSIAER
                     LY"
BASE COUNT         1599 a         2063 c         2124 g         1123 t
ORIGIN      
        1 tcaaacccga tttggtcttg atggacgtgg tcatgcccgg gcaaaacggt ttccaggcca
       61 cgcgggcgct caacaaagac gaggagacca agaacattcc ggtcatcatc gtcacatcca
      121 agagccagga aaccgacaag atctggggca tgcgccaagg cgcgaaggat tatatcgtca
      181 agccggtgga ccaggccaaa ttgctggcga aaattcgggc actgggatag atggcgaagc
      241 acttcagcct aagagaattc cagcaagggc tatcggcgcg gctgcaagcg ctggcggata
      301 ctccgtctgc ggccgcttcc aagttgggcg tgcaagctgg ccgggatttg tggctggtgg
      361 acctgattga ggccggggaa gtggtgccgt tgcccgagct tatcaaagtg ccgttgaccc
      421 gctcctggtt cagcggcgtc gccaacatcc gcggcgtgct ttacagcatc gtggatttct
      481 ccgcattcat gggcggagag ccgaccgcgc ttgggccgga tacccgggtg ctgctggtgg
      541 gacagaaact ggcggcgaac tgcggaattt tggtagaccg catgatgggc ctgcgccgta
      601 tcgagcagtt gcagcgcgtt aaaagcgccg cgcccgccgc gccgtgggcg aacgccgaat
      661 acacagacgg cgaggaccgg cgctggaagg aattgcagtt acgcaaactg gtggaacatc
      721 cggatttcct gcaaatcggg gtttgaaaac gcttaatcat cagacccggg cagtgggtcg
      781 aatccggccg aaataaagga gaagtgcatg gcattattaa aatcaaaagt gagcgaaaaa
      841 gccaaggcca gggataccaa ggatccgagt ttcgatccca tgcacaccac gtcgatcatg
      901 gacactgcca aggcgcaggc cgtgccgtgg gccctgccgc tcatcggcaa tatgcccatc
      961 aaccggcaat accagattct cggcgccttg gtggggctgt gtttggttgc cgcggcgctg
     1021 ttcttttttg ccagtgtccg cgaaacctcg aacaacgcgg tttacctgac caccgcgacg
     1081 gaaatgcaga tgctctcgca acggctggcc aagggcgcgc aacaggcggt gctcggcaac
     1141 gtaagcggtt ttgaacagtt gcggcaaggc cgcgacaagt ttggcgcaga cctcaataat
     1201 ttgatcaacg gcagtgccaa tgtgcccgcc gccaccaagc tggcacagtc taacctcgat
     1261 gaaatcagcc agttgtgggc gccgatggaa caaaaggcca gcctgattct gtcgcagaaa
     1321 accgatctgg tgaacctcgg caagaacgtg ctggcgatca acaacaacaa cccgcgcttg
     1381 ctggagctcg cgcaggaagt ggaatcaacg ttgaccacaa acaacgcgcc ggtacgcgcg
     1441 gtgcgagtgg ccagccgtca ggtgatgttg acgcaaaaac tcgcgaaaaa cgccaacgcg
     1501 ctcttgtcgg gtgagctggt ggatcccgaa gtcgcgttcc tgctggccaa ggacaccaac
     1561 actttccgcg acaatctgct ggcattaatc aacggcagcg aacaactgca catcgccccg
     1621 gccaaagacc aggaaagcct cgagcgtctt aaagatctgc aagacgcctt caaggaattc
     1681 gaaggcagcg tgacctcgat cctgcagaac cagcagaaac tcattcaggc caagcaggcc
     1741 ggacaaagta ttttcgtgga cagcgagaaa ctgctggcct cgttgcagaa gctgaccgac
     1801 cggttccggg ccgaagcgga aaaacaaggc atgctgattg ccgccatcct gttcgcggca
     1861 ctgatgctga tattcctggc cctgatgggt tttgcttcgg tgtttgacgc caggcgccgc
     1921 gctcttgaaa gtgagcggga aaacaagcgc aaccagaaag cgattttgca gctcctcaac
     1981 gacatgggtg atctggcgca gggcgacctt acggtgcacg cgcaggtgac cgcggagatc
     2041 accggcgcaa tcgccgactc catcaacttc actgtggacg aacttcgcac cctggtcacg
     2101 ggcatcaaca aggccaccgg ccaggtgacg agcgccaccg agcaggccaa agtgatttcc
     2161 acggtgcttc tcgaagcggc gcagaaacaa gcgcaggaaa ttaaggatac caccgtctcg
     2221 gtgctggaaa tggcgcgatc catgaacgag gtctcggcca acgctaacca gtcggcaaaa
     2281 gtggcgcggc agtcactcaa cgccgcggaa aagggcacgg cggcggtgca aaactcgatt
     2341 tccggcatga atgaaatccg cgagcaaatt caggaaacct cgaagcgcat caagcggctc
     2401 ggcgaatcct cgcaggaaat cggtgaaatc gtggaactga tttccgacat taccgagcaa
     2461 accaacgtgc tggcgctgaa tgccgcgatt caggccgccg ccgccggcga agcgggacgc
     2521 ggcttcacgg tggtggcgga ggaagtgcag cggctggcgg aacgctcggc ggaagcgacc
     2581 aagcagatcg gcgccatcgt gaaaacgatt cagaccgaca cccacgacgc ggtggcagcg
     2641 atggaaaaaa gtacccaggg cgtggtggaa ggagccgaac tttccgacgc cgcgggacag
     2701 gcactttcgg aaatcagcga tgtctcacgc agcctggcgc aactagcggc aacgatttcc
     2761 tccgccaccc agtcgcaagc cgaatccgca aaccgcgtgg cgaaaaacat gcaggatatc
     2821 ctcagcatta ccgaccagac cacggaaggc actaagcgga ctgcggtgtc catcggccag
     2881 ctggcgcaac tcgcggcgga actcaagagc tcggtttccg gctttaagat ttcctgattc
     2941 gttatgagcg cacagcgcga attcgacatc ggtccgctga cctgggtcaa gagcgaaatt
     3001 gaccaggcgc tgtcgcgagc gcggcaaagt ctcgaccagg cggccggcgg cgatgcggcg
     3061 ccactcaagt tctgccagac gcatctgcat caggccggcg gcgcagtgca aatggtgggc
     3121 ctcgacggcg tggcgcgttt cagcgaagaa gcggaaaggc tgctgggctt actgggaaat
     3181 cacccagaca aaattacgtc ggccacgctg gagctggtca agcgcgcgct taacgccatg
     3241 gcgcagcatc tcgacggcct gatcgacggc aaggcggacg tgccgctcaa cctcttcccg
     3301 atattcaagg aactggccca ggccgccggc accgaaaaca tttccgagag cgagctattc
     3361 ttccctgacc tgagtttgcg gccgcccagg cacccgcaag cgagcgcggt ggcggaagcc
     3421 gacatgggca atttcatcaa gaagcagcgc ggtgagttcc agcgcgggct tctcgactgg
     3481 ctgcgcaatc ccgacgattc gcagggcgtc aagcgcatgc acgagacgct ccgtgccatc
     3541 gaacagtcgc agccgctgcc ggcgcagagg actttttggt gggcggcgaa cggcttcatc
     3601 gaggcgctgg tgcacaagga ggtggaggcc gacctccacg ccaagcagct ttgcgcgcac
     3661 attgacttgc aaatccggcg tctggcggcc ggctcctaca aagtcgccga acggcaattg
     3721 cgcgaagtgc tgtacttcgt cgcgcgctgc cagccggtta ccgagcgcat ccgcgaaatc
     3781 caggaactct acgcgctcgg cagcctgctg ccggataagg cggcggtgcc gggtttgatg
     3841 gaatacgacg cttccaagct cgccccggtt ttgcgcgact ttcgcgaggc actgaccgcc
     3901 gccaaagacg cctgggccaa atacacttcg ggcgcgcggg atgcgttgcc gcgcttccgc
     3961 gagcaggtga ccaaactcat cgagctcgcc gtcgagctcg gcgcacagcc actgcaaaaa
     4021 ttgctggaag tcatcaagat tgcgctcgct cgcgcgccgg tcaagggcga ggaaaacgag
     4081 gtgctggcgc tggagatggc cactgcgctg ttgctcgcgg agagcgtcgc cgagaatctc
     4141 gccgacccgg ccgacgaact gccgcgccag atcgaagtaa tggcaagccg cttgctcgcc
     4201 gctttgggcg aaccgagcca gggcggcgag cttccggatg tgccgctcct ggacgaaatc
     4261 acccgcaagg cgcaggaaaa actcctgata gcgcaggtgg tgaacgaaat ccagtccaac
     4321 atgagccaca tcgagcaggt gctggacagc ttctttcgcg atgccgcaaa acgcaatcaa
     4381 ttgcccgcgc tcgcgccgtt cctcaagcag atagaggggg cgctcaacat cctcggtctc
     4441 gatctggcag tgaagcttct cgccgaatgc caaagattta tccagaaatt cagcgatgcc
     4501 gattacacag cgccggcgca agagctggag ctgctcgccg aaggcctgag cagcctcggc
     4561 ttttacatta atgaggtgga gaaccccgac cgcgagcaaa tcatcacgcc ggtcctgcgc
     4621 aaattcctgg atgagtcggg atccgcggcc gcgcttgaag aagcggcgcc tgaaaccacg
     4681 acgatgctca ctattgaatc cagcctcgag cggcaaaaac gcgcggcgca aaatctattc
     4741 gcctcctggc aggagaaacc gggggacgcg gtgacccggg aaaagctgca ggacaatctg
     4801 gaagccatcc gccaggatgc cgacctcgcc aagaacaagc acttgcagcg gcaggcggcc
     4861 ggcgcgctgg agctcttgaa attcgacgcc gtggccgagg acgcaaaact tgccgaaatg
     4921 atgatgacgc tcagttcgac ccaagtgcgg ccggaagcgc cgtccgagca cacccagcgc
     4981 atgctcgccg cgagcgagga agtggtggac caagaacttt tgcagatctt ccttaatgaa
     5041 gcgcaagacg tgctcaccac aatcaacgag cacctcggta gagtaaccgg cgagccgcat
     5101 aacaccgatt acctcactac catccgccgc ggcttccata ccctgaaagg cagcgggcgc
     5161 atggtggggc tgaaagactt gggcgaagtc gcgtggagca tcgaacaagt gatgaacaag
     5221 tggctgcaag aggaaagaag cgccacgcct cagctgttgt acctcatcaa gctcgcgcac
     5281 gctgcgttca gcagttgggt cagcacatta caagcccggg gccaggcgga aatcaacgct
     5341 cccaacatta tcgcgctagc cgaagccctg ctgcacggca tggaagcgga ggcggctgca
     5401 gttgccgctg ccgctgcgga agtcacggaa cttgccgccg cttccgcgcc cgaaacggtt
     5461 cctgcaaaat ccgcagtgga agaaagcatc gtgatcggcg cgacgagtct cgaacccgtc
     5521 ctctaccaga cgtttgtgga tgaagcggca gaccatatcg cctcgctcaa agacgaatgc
     5581 gcccggctgc aacaaaaccc cgagcaaagt ccgagcgaag cgctgatgcg tgcctcgcac
     5641 acgctgtccg gcatttccca caccgccggc tttaccgacc tcgccgaact gagctcggca
     5701 ctcgagcaat gggcgatgga gctcatcgaa catccggcgg gcatgagcga aatggaactc
     5761 aacgccaccg tgcaagccgt ggcgaaactg gagcagatgg tccatgcggt gcgcgaacga
     5821 cgcgtgcccg agtccgcgga aaaagaaacc caggaattgc aattgttgct ttccgccgcg
     5881 attatccaaa ccaagcccat ggtttcgacg cgattgatac aagcggagac tcctgctgcc
     5941 cgggcaccgg atgaacctgc gcctgttgca gaaaccaagt ctgcggcgtc cgctgctgaa
     6001 gccagcccga tcgaagagct gggctggacg cgcgagcgcc gggcgattcg cgacgacctt
     6061 gacgcgcagc tcctgccgat tttcctggaa gaagcgcacg acttggtgcc gatcatcggc
     6121 gaagacctgc gcagttggcg caccagcccc gaagacaaga aatcgcccat gattctcgcc
     6181 cgcgccctgc acaccctcaa aggcagcgcg cgcatggctg gcgcgatgcg gctgggcgaa
     6241 ctcacccaca acatggaaag caaggtggag aacgcggcac agttgggcca accgatgatg
     6301 tcgctgttcg acgaccttga aaaccagttc gaccgcatgg ttgcggaaat cgagaaaatc
     6361 aagagcggcg cgcaacccga agccgcgccc gtggctgaag cggcggccgc cgaagccgcc
     6421 cccaccgagg cgacagagcc tgaaccgatt caagtgcggg cgatgctcag agtgcgcgcc
     6481 gatatcgttg accggctggt gaacgaggcg ggggaagtca gcatcgcgcg cgccaggatc
     6541 gaaggcgaaa tgcgcgactt caagcaatcg ctgctggaat tgaccgaaag cgtggcgcgg
     6601 ctgcgccacc agttgcgcga agtggaaatc caggcggaaa cgcagatgca gtcgcgcatg
     6661 agccagatgg gcgaggaagc gcagcgtttc gacccgctgg agttcgaccg tttcacccgc
     6721 ttgcaggaat tgacccgcct gatggcggaa agcgtgcacg acgtcgtcac cgtgcagcag
     6781 acgctgctca agaacgtgga cgaaaccgac gcggcgattc tgcagcaggc gcggctcaat
     6841 cgcgatctcc aggatgaact gatgcgcgtg cgcacaatgc cgttttccag catcgcggaa
     6901 cggttgtac
//
LOCUS       DATIOW010000169         3139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_73580, whole genome shotgun sequence.
ACCESSION   DATIOW010000169 DATIOW010000000
VERSION     DATIOW010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73580"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VLL03_05650"
     CDS             <1..579
                     /locus_tag="VLL03_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSS46882.1"
                     /translation="RFDTWLLIDDAHGFGVLGKEGRGALSHFNIRSPRIVYMGTLGKA
                     AGVFGAFVAAEKEVIATLIQLAHSYIYTTASPPLLAHVLLKSLDLINAGNGRRAHLAQ
                     LIVQLKKNLRLKSWRLAQSQTPIQPLIIGESRAAVRASKALAEAGFLVPAIRPPTVPQ
                     STARLRISLSAAHSVDDVERLVAALHELERAI"
     gene            672..1436
                     /gene="bioH"
                     /locus_tag="VLL03_05655"
     CDS             672..1436
                     /gene="bioH"
                     /locus_tag="VLL03_05655"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR01738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HSS46883.1"
                     /translation="MTLYIESTGDGPDLMLLHGWGMHSGVWDGVYAALAKRFRVHRVD
                     FPGYGKSDGCTRHTLIEFVQSLAKAAPESVAVCGWSLGAQVALAWARNFERQVKQLVL
                     VAATPRFVRAPDWPHGMKTQVFAEFAAELNRDGRAALKRFVSLQTRGAEDARSQSAWL
                     RQQLFAHGAPEQSALETGLRILRDTDLRETAQRLRQPVLLLHGAADALVPLGAALWLK
                     QHFPQARLEVFERCAHAPFLSKPGEFVKELTEFLDG"
     gene            1429..2301
                     /gene="bioC"
                     /locus_tag="VLL03_05660"
     CDS             1429..2301
                     /gene="bioC"
                     /locus_tag="VLL03_05660"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: protein motif:HMM:TIGR02072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102130 - malonyl-CoA methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HSS46884.1"
                     /translation="MGETDPFALDKRLVRLSFERAAATYDQAAVLQREVGARMLSRLD
                     YIKLTPNSVLDAGSGTGHGSRELKQRYPRAKVLALDIALAMLERSRRHGRWLPFLPQP
                     IVHLCADIERMPLKAASAGMVWCNLALQWVNQPQRAFAEMHHVLQPGGLLMFSTFGPD
                     TLKELRQAYSGIDGYSHVHRFIDMHDLGDMLVQTGFNTPVMDMEQITLTYVNVVSLMH
                     DLKAIGAHNVSHGRRHGLAGKSLLQTVSRNYEKFRSEGRLPATFEVVYGHAWKPEAPH
                     MPDGRKIIKVEARR"
     gene            2301..2978
                     /gene="bioD"
                     /locus_tag="VLL03_05665"
     CDS             2301..2978
                     /gene="bioD"
                     /locus_tag="VLL03_05665"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HSS46885.1"
                     /translation="MSRGFFVTGTDTGVGKTLVACALLHAFAALGKSTIGMKPVAAGG
                     EESAQGLRHDDVEALLAASSIKAPRNLINPYALKPAIAPHIAARRAGIEIDLPVIKNA
                     FSQLVGMADVVIVEGVGGFKVPLTSRADSADLATLLGLPVILVVGMRLGCLNHALLTA
                     EAIESRGLQLVAWVANCLDPQMQALEENLAALKERLKQAPLGVLEFAASLQPVEAAAD
                     LTVSSLI"
BASE COUNT          637 a          840 c          976 g          686 t
ORIGIN      
        1 cgtttcgata cctggctgct gatcgacgat gcgcacggtt tcggcgtgct gggcaaggag
       61 ggacgaggcg cgctttcgca tttcaatata cgttctccgc gcatcgttta catggggacg
      121 ctcggcaagg cggccggcgt cttcggcgct tttgttgctg ccgaaaaaga agtaatagca
      181 acgctcatcc aacttgccca cagctatatt tacaccaccg cctcgccgcc gctgctggcg
      241 catgtgctgc tgaaaagcct ggatttgatt aacgcaggaa acggtcgccg ggcgcatctt
      301 gcgcaattga ttgtacagtt gaagaaaaat ttgcggctga aaagctggcg tctggctcaa
      361 tcccaaacgc cgattcagcc gctgatcatc ggcgaatcgc gcgcagcggt gagggcgagc
      421 aaagcactgg ctgaagcagg cttcctagtt ccggcaatcc gtccgccgac cgtgcctcaa
      481 agcacggcgc ggcttcggat ttcgctttcc gcagcccatt ccgtggacga cgtcgagcga
      541 ttggtagcag cattgcatga attagaaaga gcaatttaac cgcggaggat gcagaggaaa
      601 cttgattcct agtaaatatt atggtttttc ctctgcgttc tccgcgccct ccgcggtgaa
      661 aaggttttga attgactttg tacattgaat ccactggcga tggccccgat cttatgctgc
      721 tgcacggctg gggcatgcac agcggcgtgt gggacggtgt gtatgcggcg ctggcaaaac
      781 gctttcgcgt ccatcgcgtg gattttcccg gctatggcaa aagcgacggt tgcacgcggc
      841 atacgctcat agagttcgtg cagtcgctgg ccaaggccgc gcctgaaagc gtggcagtgt
      901 gcggttggtc gctgggggcg caagtggcgc tggcctgggc gcggaatttt gagcggcagg
      961 taaaacaatt ggtattggtg gcggccacgc cgcgctttgt gcgcgccccc gattggccgc
     1021 atggaatgaa aacgcaggta tttgcggaat ttgcagctga actcaaccgc gacggccggg
     1081 cggcgctgaa gcgcttcgta tctttgcaaa cccggggcgc ggaggatgcg cgcagtcaaa
     1141 gcgcttggct gcgccagcaa ctgttcgcgc acggcgcgcc tgagcagtcc gcattggaga
     1201 cgggcctgcg gattttgcgc gacacggatt tgcgggaaac ggcgcagagg ctgcgtcagc
     1261 cggttttgct tttgcatggc gcagccgatg cgctggttcc gcttggcgcg gcgttatggc
     1321 tgaagcagca ttttccgcag gccaggcttg aagtatttga gcgttgcgct catgcgccgt
     1381 ttttgtcaaa gcctggagaa ttcgtgaaag aacttacgga attcctggat gggtgaaacc
     1441 gatccgtttg ccttggacaa gcgtctggtg cggctttctt tcgagcgcgc ggcggccact
     1501 tacgatcagg ccgcggtgct gcagcgcgaa gtgggggcgc gcatgctttc gcgtctggat
     1561 tatatcaaac tcacgccgaa ttccgttctc gatgctggca gcggcaccgg ccatggcagc
     1621 cgcgaactca agcagcgtta tcctcgggca aaggtactgg cgctcgacat cgccttggcc
     1681 atgctcgagc gttcacgaag acacgggcgc tggctgcctt ttctgccgca gcccatcgtt
     1741 catttatgtg ccgacatcga acgcatgcca ctcaaggcag caagcgccgg catggtgtgg
     1801 tgcaacctgg cgctgcaatg ggtaaatcag ccgcaacggg cattcgccga aatgcaccac
     1861 gtattgcagc cgggagggct gctgatgttc agcaccttcg gaccggacac gctcaaagag
     1921 ctgcggcagg cgtactcggg gatcgacggc tacagccatg tgcatcgctt tattgacatg
     1981 catgacttgg gtgacatgct ggtgcaaacg ggattcaata ccccggtgat ggacatggaa
     2041 caaatcacgc tgacttatgt gaatgtcgtt tccttgatgc acgatctcaa ggccatcggc
     2101 gcgcacaacg taagtcatgg gcgtcgtcat ggcctggcgg gcaagtcgct actgcaaacg
     2161 gtgtcgcgca attacgagaa gtttcgcagc gaggggcgtc tgccggcgac gtttgaagtg
     2221 gtatatggtc atgcctggaa gcctgaagcg ccgcacatgc cggatggaag gaaaatcatc
     2281 aaagtagaag ccagacgttg atgagccggg gtttttttgt caccggcacc gataccggcg
     2341 tgggcaagac gctggtggcc tgtgctttgc tgcatgcttt cgcggcttta ggaaaaagca
     2401 cgatcggcat gaaaccggtc gcggcgggcg gcgaggaatc cgcgcaaggt ttgcgccatg
     2461 acgatgttga agccttgctt gccgcaagct ccattaaggc gccccgcaat ttgattaatc
     2521 cttacgcgtt gaagccggcg attgcgccgc atatcgctgc gcgacgggcc ggaatcgaaa
     2581 tcgatttgcc ggtcatcaaa aacgcattca gtcaattagt cggaatggcc gacgttgtga
     2641 ttgtggaggg cgtaggcggt ttcaaggtcc cgctcacttc ccgcgcagac agcgccgatc
     2701 tcgccacgtt acttggtctt cctgtaattc tggtggtagg gatgcgtttg ggctgcctca
     2761 accacgcctt gcttaccgca gaagcgatcg agtcgcgagg attgcaactc gtagcctggg
     2821 tcgctaattg cctggacccg caaatgcaag cgctcgaaga aaatcttgcc gcgcttaaag
     2881 aaaggcttaa gcaagccccc ttgggtgtgc tcgaatttgc ggcgagtctg cagcctgtgg
     2941 aagcggcggc ggaccttacg gtttcctctt tgatttgata gacaaagcag agcagaaaaa
     3001 agcctgattg tccttgccat gccagcttgc ctatgctaat ttacgcgctt gttgaattgg
     3061 gcaaggcggg tcgggaggca cgagaacatg cagtgcaccg gtgcggatga tgcggcggct
     3121 tacacgctca ccgcaaaac
//
LOCUS       DATIOW010000170         2642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1700141, whole genome shotgun sequence.
ACCESSION   DATIOW010000170 DATIOW010000000
VERSION     DATIOW010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700141"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VLL03_05670"
     CDS             complement(<1..304)
                     /locus_tag="VLL03_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HSS46886.1"
                     /translation="MTSERTRTRMVERLRSQGILDEIVLTAMATVPRHIFVDEALASR
                     AYEDVSLPLGFGQTISSPYIVARMLELLRGGKPLKRALEVGTGCGYQAAVLARLADE"
     gene            complement(333..1079)
                     /gene="surE"
                     /locus_tag="VLL03_05675"
     CDS             complement(333..1079)
                     /gene="surE"
                     /locus_tag="VLL03_05675"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HSS46887.1"
                     /translation="MRILLSNDDGYFAPGLATLAETLSRIAEVTVVAPERDSSGASNS
                     LTLDRPLSLHQSQGGFYYVNGMPTDCVHLAVTGMLERLPDMVISGINDGANMGDDTIY
                     SGTVAAATEGFLLGIPSLAVSLVTQSAVHYATAAQVAVEIVKRFEKRPHSQPVLLNVN
                     VPDVEYSKLNGLEITRLGRRHKAEPVVKTRNPRGETVYWVGAAGGVQDAGAGTDFHAV
                     AQNKVSVTPLQIDLTQFAQMESLRRWLEDF"
     gene            complement(1203..1279)
                     /locus_tag="VLL03_05680"
     tRNA            complement(1203..1279)
                     /locus_tag="VLL03_05680"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1243..1245),aa:Pro,seq:ggg)
     gene            complement(1323..1682)
                     /locus_tag="VLL03_05685"
     CDS             complement(1323..1682)
                     /locus_tag="VLL03_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HSS46888.1"
                     /translation="METSRKEALPPIPAKRYFTIGEVSELCGVKPHVLRYWEQEFTQL
                     KPVKRRGNRRYYQHHEVLLIRRIRELLYEQGFTINGARNRLDETVGIPEKPIAHLKFD
                     FSLVRREIKSVLEILQR"
     gene            complement(1666..1968)
                     /locus_tag="VLL03_05690"
     CDS             complement(1666..1968)
                     /locus_tag="VLL03_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HSS46889.1"
                     /translation="MTLTKAELADLLFEKVGLNKREAKDMVESFFEEVRAALENGESV
                     KLSGFGNFQLRDKPQRPGRNPKTGEEIPITARRVVTFHASQKLKLLVEKNYHGNKS"
     gene            complement(1977..>2642)
                     /locus_tag="VLL03_05695"
     CDS             complement(1977..>2642)
                     /locus_tag="VLL03_05695"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HSS46890.1"
                     /translation="RLFEISRCFTKDKSGYVQPEKLAGLCYGLYLPEQWGAPSRNVDF
                     FDVKADLEALFWPLAPRFVAGRHAALHPGRAAQVWAGERLAGWVGELHPQLQHKYELP
                     QPPVVFELELEILMQRESPLAQEVSKFPPVRRDIAVVVDAQANVQAMLESLQAGKPLT
                     VTEIALFDLYQGEGIDSGKKSLAFRVLLQDTQKTLTDVEVEAAVAQLKQILQQEYHAK
                     LRT"
BASE COUNT          512 a          799 c          774 g          557 t
ORIGIN      
        1 cttcatcggc gaggcgcgcc agcacggccg cttggtaacc gcaaccggtg ccgacttcca
       61 gcgcgcgttt taacggcttg ccgccgcgca acagttccag catgcgcgcc acgatgtacg
      121 ggctggaaat agtctgacca aagcccagcg gcagcgagac atcctcataa gcgcggcttg
      181 ccagcgcctc atccacgaaa atgtggcgcg gcactgtcgc catggcagtg agcactatct
      241 cgtcaagtat gccctggctg cgcaggcgct ccaccatgcg cgtgcgcgtg cgctccgaag
      301 tcatgccgat gccggagtgc cgcgcggtca tgctagaagt cctcaagcca gcgcctgagg
      361 ctttccatct gcgcaaactg ggtgagatca atctgtagcg gcgtgaccga cactttgttt
      421 tgcgccacgg cgtgaaaatc ggtgccggca ccggcatcct gcaccccgcc tgccgcgccc
      481 acccaataca ccgtctcgcc gcgcggattg cgggtcttca ccaccggctc cgccttgtgg
      541 cgccggccaa gccgggtgat ttcaagtcca ttgagtttgg aatactcaac atccggcacg
      601 ttgacgttaa gcagcaccgg ctgggaatgg ggacgttttt cgaagcgctt gacgatttcc
      661 acggcgactt gcgccgcggt ggcgtaatgt accgcggatt gggtgaccag cgacaccgcc
      721 agcgaaggaa tgccgagcag aaagccctcg gtcgccgccg ccacggtgcc ggaataaatc
      781 gtgtcatcgc ccatgttggc gccgtcgttg atgccggaaa tcaccatgtc gggcaatcgc
      841 tccagcatgc cggtcaccgc cagatgcacg caatcggtgg gcatgccgtt cacgtagtaa
      901 aaaccgcctt gggactggtg caggctcagc ggccggtcga gagtgaggga gttgctggcg
      961 ccgctgctgt cgcgctccgg cgccaccacc gtgacttcgg cgatgcgtga cagcgtctcg
     1021 gcgagcgtgg cgagccccgg cgcaaaatag ccgtcgtcgt tactgagcag gatgcgcatc
     1081 gcagaaaggt attagcaggc gacagaaggc aggcatgcgg cgggagagga agcgatgagc
     1141 ccgcagggcg acactcaagg tttcgatttc ttaacccctg ttttctgtct tctgggttga
     1201 actggtcggg gcggcgagat tcgaactcgc gaccacttgc accccatgca agtacgctac
     1261 caggctgcgc tacgccccgt cagcgaaaaa ttataccaga accgggagag aggaaactgg
     1321 catcagcgct gcagaatttc gaggacgctt ttgatttcgc ggcgcactag agaaaaatcg
     1381 aatttgaggt gggcgatcgg tttttccggg atgcccaccg tttcatcgag gcggttgcgc
     1441 gccccgttga tggtaaagcc ctgctcgtag aggagttcgc ggatgcggcg gatcagcagc
     1501 acttcatggt gctggtaata gcggcggttg ccgcggcgct tgaccggctt caactgggtg
     1561 aattcctgct cccagtagcg cagcacatgc ggtttgaccc cgcacaactc gcttacttcg
     1621 ccgatggtaa agtagcgctt ggcgggtatt ggcggtaacg cctccttacg acttgtttcc
     1681 atggtagttt ttttccacca gcagcttcag cttctgactg gcgtggaagg tgaccacccg
     1741 gcgtgcggtg atggggattt cctccccggt cttgggattg cgccccggac gttgcggctt
     1801 gtcgcgcagc tggaaattgc cgaatccgga gagcttgacg ctttccccgt tttccaatgc
     1861 ggcgcgcacc tcctcgaaaa acgattccac catgtccttg gcttcccgct tgttcagccc
     1921 gactttttca aacagcaagt cagcgagttc ggctttggtc agcgtcatac ttccccctag
     1981 gtacgtaact tggcgtggta ttcttgttgc agaatttgct ttagttgcgc gaccgccgcc
     2041 tcgacctcta cgtcagtcaa tgttttttga gtatcttgta ataatacacg aaatgcaaga
     2101 ctttttttac cagaatcaat gccttcgccc tgatagaggt caaacagcgc aatctccgtg
     2161 actgttagcg gcttgccggc ctgcaggctc tccagcatgg cctgaacgtt tgcttgggcg
     2221 tccactacca ccgcaatgtc gcgcctcacc ggcgggaatt tggagacttc ctgtgccagc
     2281 ggcgactcgc gctgcattaa aatctccagt tccagttcaa acaccaccgg cggctgcggc
     2341 agttcgtatt tatgctgcaa ctgcggatgc agctctccca cccagccagc taatctctcc
     2401 cccgcccaga cttgcgccgc acggccggga tgcagcgccg cgtgccggcc cgcaacaaag
     2461 cgcggggcca gcggccagaa cagggcttcg aggtcggctt tgacgtcgaa aaaatccacg
     2521 ttacgcgagg gcgcgcccca ttgttcaggc agatacaatc cgtagcaaag acccgccagc
     2581 ttttcgggct ggacatatcc ggatttatct ttcgtgaagc aacgactgat ttcgaacagg
     2641 cg
//
LOCUS       DATIOW010000171         3063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1902016, whole genome shotgun sequence.
ACCESSION   DATIOW010000171 DATIOW010000000
VERSION     DATIOW010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1902016"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VLL03_05700"
     CDS             <1..525
                     /locus_tag="VLL03_05700"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HSS46891.1"
                     /translation="KKLGLPLMVKPSCEGSSIGMSKVMKVEKLGAAYRLAAKHDSVVI
                     AEKFIKGMELTASILDGQVLPLIRVETPRAFYDYQAKYFATSTRYICPCGLSAKREKK
                     IQERALQAFRILGCSGWGRVDLMLDKRRVPYFIEANTVPGMTDHSLVPMAARAAGLSF
                     EDLVMRILESAHVG"
     gene            515..1258
                     /locus_tag="VLL03_05705"
     CDS             515..1258
                     /locus_tag="VLL03_05705"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HSS46892.1"
                     /translation="MWDKPYLMLWLANLLYTLAALLALYAALNVATRLPLFDLREVVV
                     NGELEHMTRGQVQQAVRQLKGNFFTLDLNQARSGFEKLPWVRSVNLRREWPDRLEVVL
                     EEQVALARWDGGGLVNTYGEVFAGGTERLLPLFSGPAGSAPDITRQYLLFSRELAPIN
                     LKPVQVQMSQRRAWQVRLDNGLLLVLGRERSEARFKKFVAAYNRTIAQLQWQPEYADL
                     RYDNGFVLRVPEAVRQQSEPAKREKRKPA"
     gene            1274..2518
                     /gene="ftsA"
                     /locus_tag="VLL03_05710"
     CDS             1274..2518
                     /gene="ftsA"
                     /locus_tag="VLL03_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HSS46893.1"
                     /translation="MVISRGKENKDLIVGLDIGTSKIAAIVAEVRPEGGLEIIGMGSA
                     PSRGLKKGVVVNIESTVNAIQRALEEAELMADCKIREVYTGIAGSHIKSFNSHGMVGI
                     KDKEVTQMDVERVVETAKAVNIPTDQQILHILNQEFIIDGQEDVREPLGMSGVRLEVK
                     VHIVTGAVSAAQNIMKCVRRCGLSVRDLILQPLASATAALSEDEKDLGVCLADIGGGT
                     ADIAVFTHGAIRHTAVIPIAGDQITNDIAMALRTPTKDAEDIKRRFGCALRQLADPHE
                     MVEVPGVGDRGPRQLSRQTLAEVIEPRVEELYSLIQAELRRSGFEELLSSGIVITGGS
                     SAMQGMVELGEEVFHMPVRLGMPHYLGGLAEVVRHPRFSTGVGLLLIGMQQRQRQELV
                     KLQSGSLRPIFERMKNWFQGNF"
     gene            2615..>3063
                     /locus_tag="VLL03_05715"
     CDS             2615..>3063
                     /locus_tag="VLL03_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="GTPase; similar structure to tubulin; forms
                     ring-shaped polymers at the site of cell division; other
                     proteins such as FtsA, ZipA, and ZapA, interact with and
                     regulate FtsZ function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HSS46894.1"
                     /translation="MFEIMDTQGQEAVIKVIGVGGCGGNAIDHMINQGVKGVEFICAN
                     TDVQALKRNQAKIQLQMGASITKGLGAGANPDIGREAALEDRERIAELIEGADMLFLT
                     AGMGGGTGTGAAPVVAEIAKDLGILTVAVVTKPFAFEGKRMRIAQQGV"
BASE COUNT          708 a          757 c         1001 g          597 t
ORIGIN      
        1 aaaaaactcg gattgccatt gatggtgaaa ccatcatgcg aaggctcaag catcggcatg
       61 agcaaagtga tgaaggtgga aaaattgggc gcggcttacc gactcgccgc aaagcatgac
      121 agcgtggtga tcgccgagaa attcatcaaa ggcatggaat tgactgcctc gattctcgat
      181 ggccaggtgc tgcctctgat acgggtggaa acgccgcgtg ccttttacga ttaccaggcg
      241 aaatattttg ccaccagcac gcgctatatc tgcccgtgcg ggctcagcgc aaagcgggaa
      301 aaaaaaattc aggagagagc actgcaggca ttccgcatct tgggttgcag cggctggggc
      361 agagtggacc tgatgctgga caagcggcgt gtgccatatt ttatcgaagc caacaccgtg
      421 ccgggcatga ccgatcacag cctggtgccg atggcggcgc gcgcagcagg tttgagcttc
      481 gaagatctgg tgatgcggat tttggagtcg gcgcatgtgg gataagcctt acttgatgct
      541 gtggcttgcc aatctgcttt atacgcttgc cgcgttgctc gctctttatg cggcgcttaa
      601 tgtggcaacc cgcctgccgt tgtttgattt gcgtgaagtg gtggtgaacg gggagcttga
      661 gcatatgaca cgcgggcaag tgcagcaggc ggtacggcaa ctcaagggaa attttttcac
      721 actggatttg aatcaggcgc gcagcggctt cgaaaaactg ccgtgggtga gaagcgttaa
      781 cctgcgccgc gagtggcctg accgtttgga agtggtgttg gaagaacagg tggcgctggc
      841 gcgctgggac ggaggcggac tcgtcaatac ttatggcgag gtttttgcag gcgggacgga
      901 gcggctattg ccgctgtttt ccggccctgc cggaagcgca ccggatatca ccaggcaata
      961 cttgctgttc agccgcgaac tcgcaccaat caacctgaag ccggtgcagg tgcaaatgtc
     1021 acagcgccgg gcatggcagg tgcggctgga caacggcttg ttgctggtac tgggaagaga
     1081 gcgtagtgaa gcgcggttca aaaaattcgt cgctgcatac aaccgaacca tagcacagct
     1141 gcaatggcag ccggaatacg ctgatttgcg ttatgacaac ggattcgtgc tgcgcgtgcc
     1201 tgaggcggtg agacagcaga gcgagcccgc caaacgagaa aaacgcaagc cagcctgaga
     1261 ggatttacag aggatggtaa taagccgggg gaaggaaaac aaggatctga tcgtgggcct
     1321 cgacatcggg acctcgaaaa tcgctgcgat cgtcgcagaa gttcgccccg agggcgggct
     1381 ggaaatcatc ggcatgggca gcgcgccttc gcgcggctta aagaaaggcg tagtggtgaa
     1441 cattgaatcc acggtgaacg ccatccagcg cgcgctggaa gaagcggagt tgatggccga
     1501 ttgcaagatc cgtgaagtgt acacgggaat tgccggcagc cacatcaaga gcttcaattc
     1561 tcacggcatg gtgggcatca aggacaagga agttacgcag atggatgtgg aacgcgtggt
     1621 ggaaaccgcc aaggcggtga atatccccac cgaccagcag atcctgcaca tccttaacca
     1681 ggaattcatc attgacggcc aggaagacgt gcgcgagccg ttgggcatga gcggagtgcg
     1741 gctggaagtg aaggtgcaca tcgtgaccgg cgcggtctcg gcggcgcaga acatcatgaa
     1801 gtgcgtacga cgctgcgggc tttcggtgcg cgatttgatt ttgcagccgc tggcctcggc
     1861 cacagcggcg ctgtcggaag atgaaaaaga tctgggcgta tgcctggcgg atatcggcgg
     1921 aggcaccgcg gatatcgcgg tgttcactca tggcgcgatc cgccataccg cggtgatccc
     1981 gattgccggc gatcagatca ccaacgatat cgcgatggcg ttgaggacac cgaccaagga
     2041 tgccgaggac atcaagcgcc gcttcggctg cgcgctgcgg cagctcgctg atccgcacga
     2101 gatggtggaa gtccccgggg tgggtgaccg gggcccgcgt caattatccc ggcagacact
     2161 cgccgaagtc atcgagccgc gtgtcgagga actttattcg ctgattcagg cggaattgcg
     2221 tagaagcggt ttcgaggaat tgctgtcttc cggaatcgta atcaccggcg gcagcagtgc
     2281 catgcagggc atggtggaat tgggtgaaga agtattccat atgccggtgc ggctcggcat
     2341 gccgcactat ctgggcggac ttgccgaagt ggtacgtcac ccgcgttttt ccactggggt
     2401 gggcttgctg ctcattggca tgcagcagcg ccagcggcag gaactggtca agctgcagag
     2461 cggttctttg cgcccgatct tcgagcgcat gaagaattgg ttccagggga atttttaata
     2521 atgaaattca agaaaaaaac gggagcgaga caggttatcg gtaatcaagg gagattttac
     2581 tgtaattgga atcactggca taaaggaggc acacatgttt gaaatcatgg atacacaagg
     2641 gcaggaagcg gtaatcaagg tgattggggt cggcggctgc ggcggcaacg ccattgatca
     2701 tatgatcaac cagggagtga aaggcgtgga attcatctgc gccaataccg atgtgcaggc
     2761 gttgaagcgc aaccaggcga aaatccagct gcaaatgggc gcctccatca ccaagggact
     2821 tggcgcaggg gccaatcccg acatcgggcg cgaagcggca ctggaagatc gcgagcgtat
     2881 tgctgagctg atagaaggcg ccgacatgct gtttttgact gcgggcatgg gcggaggcac
     2941 cggcaccggg gccgctccgg tggtggcgga aatcgccaaa gacctgggca tcctcaccgt
     3001 agcggtggtg accaagcctt ttgcttttga aggcaagcga atgcgcatcg cgcaacaggg
     3061 cgt
//
LOCUS       DATIOW010000172         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1295820, whole genome shotgun sequence.
ACCESSION   DATIOW010000172 DATIOW010000000
VERSION     DATIOW010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295820"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VLL03_05720"
     CDS             <1..202
                     /locus_tag="VLL03_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46895.1"
                     /translation="AAINDELAVTVVPAESGGSRIVFAQSVRRGAVVLVGARVKVACV
                     NPDSFKPVKMPDVMIKALGKNL"
     gene            199..882
                     /gene="tolQ"
                     /locus_tag="VLL03_05725"
     CDS             199..882
                     /gene="tolQ"
                     /locus_tag="VLL03_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010396146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HSS46896.1"
                     /translation="MNVTQDLSLMGMVTNASVLVQLVILLLLVVSLMSWWYIFRKMFV
                     INSATRQSNEFEKNFWSGRDLNSLYQRAAGSRHSAGSLEKIFDAGFREYAKLKRQPGM
                     NISVVMDGTRRAMRATYQREMDRLEAHLSFLATVGSVSPYVGLLGTVWGIMNAFRGLS
                     NVVQATLAHVAPGIAEALVATAMGLFAAIPAVVAFNRYARDIDRLATRFESFMEEFSN
                     ILQRQTPAS"
     gene            879..1301
                     /gene="tolR"
                     /locus_tag="VLL03_05730"
     CDS             879..1301
                     /gene="tolR"
                     /locus_tag="VLL03_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HSS46897.1"
                     /translation="MMERRQPRRLMNQINVVPYIDVMLVLLVIFMITAPLINPGQIDL
                     PQIGKSLAPPVAPLEVGIKANKTLLLRDRSTTQSERVVSQDELIKAIKEKQSKNPDQP
                     VVIAADKNVRYEEVLKVMDVLQQNQVKKIGLLAKPRTS"
     gene            1301..2131
                     /gene="tolA"
                     /locus_tag="VLL03_05735"
     CDS             1301..2131
                     /gene="tolA"
                     /locus_tag="VLL03_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HSS46898.1"
                     /translation="MRSAAEREPGRTASFILAVLMHLLFIGLLVFGISWQNKQPQVTE
                     VDLWNKLPAPPQPVELPKPVKEVKPPPEPPKQEVKPEPKPEPVVKSEPKPEPVKADIL
                     LKQKLEKEKAEKIEKEKAAKEKRKLEEQKLAEEKRAVEAERRQIQEQLAKEEDARRLQ
                     QEQKEALEALQKDQMAAQAAAQDRLLKDYTDRIHAKIHQYVVVPPDIQGNPEATYKVV
                     LLPGGDVLSAALVKSSGVPAYDSSIERAIYKAQPLPLPPDPALFAKFRELELKFRPND
                     "
BASE COUNT          532 a          568 c          674 g          413 t
ORIGIN      
        1 agcagcgatc aatgatgagc tggcggtaac ggtggttccg gcggaaagcg gtgggagccg
       61 aattgttttt gcgcaaagcg tgcgccgcgg cgccgtcgtg ctggtcggcg ctcgggtcaa
      121 ggtggcatgc gtcaaccccg attcattcaa gccggttaaa atgccggatg tgatgataaa
      181 agcactggga aaaaacctgt gaatgtaact caagatttgt ccttgatggg catggtcacc
      241 aatgccagcg tgctggtgca gttggtaata ctgttgttgt tggtggtatc gctaatgtcc
      301 tggtggtaca tcttccgcaa gatgttcgtg atcaatagcg ccacgcggca gtcaaacgag
      361 tttgaaaaga atttctggag cggaagggat ttgaattcgc tctatcagcg cgccgcgggc
      421 tcacgccaca gcgccggcag cctggagaaa atcttcgatg cgggcttccg cgaatacgcc
      481 aagctcaagc gccagccggg aatgaatatc agcgtggtga tggacggcac gcgccgcgcc
      541 atgcgcgcga cttatcaaag agagatggac cggctggaag cgcatctttc gtttctcgcc
      601 acggtgggtt cggtcagtcc ctatgtcggc ttgttaggca cggtgtgggg aatcatgaac
      661 gcgtttcgcg gtctttccaa cgtggtgcag gcgacgctcg cacatgtcgc gccgggaatt
      721 gcggaggcgc tggtcgccac cgccatgggc ctgtttgcgg cgattcccgc agtggtggcg
      781 tttaaccgct atgcgcgcga catcgaccgg ctcgccaccc gctttgaaag cttcatggaa
      841 gaattctcga acatcctgca aaggcaaaca cccgcgtcat gatggaacgc cgccaaccgc
      901 gccggctgat gaaccagatc aacgtcgtgc cgtacatcga cgtgatgctg gtgctgctgg
      961 tgattttcat gattaccgct ccgctcatca atccggggca gattgatcta ccgcaaatcg
     1021 gcaaatcgct cgctccgccg gtagcgccgt tggaagtggg tatcaaggcc aacaaaaccc
     1081 tgcttctgcg cgaccgctcg accacgcaat cggagcgggt ggtttcgcag gacgagttga
     1141 tcaaggcgat caaggagaag cagagcaaga atcccgatca gccggtggtg attgccgcgg
     1201 acaagaacgt gcgctacgaa gaagtactga aagtgatgga tgtgctgcag caaaatcaag
     1261 tgaaaaaaat cgggctgctg gcaaagccga ggaccagctg atgcgctccg cggcggaacg
     1321 cgagccgggc cgcaccgcct cattcatact ggcggtgctg atgcatctgc tgtttatcgg
     1381 gctgctggtt ttcggcatca gctggcaaaa caaacagccg caagttaccg aagtggatct
     1441 gtggaacaaa ttgccggcgc cgccccaacc ggttgagttg ccaaaacccg taaaagaagt
     1501 caagccccct cccgagcctc ccaagcagga agttaaaccc gaaccgaaac cggagccggt
     1561 ggtgaaatcc gagcctaagc cggagccggt gaaggccgat attctgctca agcagaaatt
     1621 ggaaaaagag aaagcggaaa aaatcgagaa ggaaaaagca gcgaaagaaa aacgtaagct
     1681 agaggagcag aagctcgctg aggaaaagcg cgcggttgaa gcagagcggc gccagattca
     1741 agaacagctg gcgaaggaag aagatgcgcg gcgcttgcag caggagcaga aggaggcgct
     1801 tgaagcgctg cagaaggatc agatggcggc gcaggcggcg gcgcaggatc gcctgctcaa
     1861 ggactatacg gaccggattc acgccaagat acaccaatat gtggtggtac ctccggatat
     1921 tcagggcaac cctgaagcga catacaaggt ggtgctgctt ccggggggcg atgtgttaag
     1981 cgccgcactg gtgaaaagca gcggcgttcc cgcgtatgac agctcgatcg agcgcgccat
     2041 atacaaggct cagccgctgc cgcttccgcc cgacccagcg ctgtttgcga agttccgcga
     2101 actggaactc aagttccgtc ccaatgacta agggttaaac tgatataatt gtcgcgatga
     2161 cgtttagatt aagaaagcgc atttgct
//
LOCUS       DATIOW010000173         3261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1903627, whole genome shotgun sequence.
ACCESSION   DATIOW010000173 DATIOW010000000
VERSION     DATIOW010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903627"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VLL03_05740"
     CDS             <1..789
                     /locus_tag="VLL03_05740"
                     /inference="COORDINATES: protein motif:HMM:NF021646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46899.1"
                     /translation="NEYWLRADPVHLRVDSGQLVLADSSALDITQDEANRLTQALNSH
                     FNNAGLVFQAMHPERWYLRLEKLPKLQTRALAEVAGKNIDEFLPIGADSIYWHGVCNE
                     IQMVLHHHAVNEAREAVGNPPVNSVWLWGGGLLPKITGNPFAHVWTNECLAKGLALAS
                     GAGLSRLPQNAQAWLAQPHAPGVHLVVLDSLRSAAQYRDRERWLENIKELEACWFAPL
                     LTALQRGQLDELVLSSGPWSFAIARSDLWKLWRRGKAFANYALG"
     gene            791..2488
                     /gene="recJ"
                     /locus_tag="VLL03_05745"
     CDS             791..2488
                     /gene="recJ"
                     /locus_tag="VLL03_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HSS46900.1"
                     /translation="MLKIIPRPCPKPAFEALLSQEMHPVLARIYAARGIQSPVQLKSE
                     LSSLVPFDRLKNAAQMAHILADAIAAKKRLVIIADYDADGATACAVGMRALREFGALV
                     DYLVPNRFEYGYGLTPEIVQLAKQVKRPDILITVDNGIAAVEGVAEASRLGMATLITD
                     HHLPGAELPQAACIVNPNQPECEFPSKNLAGVGVMFYVMLALRAELRALHAFDSRAEP
                     NLSKLLDLVALGTVADVVKLDDNNRILVSQGLRRMREAKAHAGVQALFRAAGRDPLRA
                     STYDLAFMLGPRLNAAGRLDDMSLGIECLVSDDAEFAATAARRLDQLNRERRDIEAGM
                     QQAALAALERIEARDSVSLCLFDPNWHPGVVGLLASRIKDKFHRPVIAFAPAGDGGIK
                     GSGRAISGLHLRDALDLVAKRHPGLILKFGGHAAAAGLSIRQNDFERFSLAFEETVAA
                     LISPADLERVIETDGTLAIADMQIELARLLEKEVWGQGFPQPSFSSDFTVENQRVVGE
                     KHLKLKLSLSGRAFDAMLFFHSKPLPERILAVYRLAVNEYNSAQSLQLNIEHWQPAA"
     gene            2503..3261
                     /locus_tag="VLL03_05750"
                     /pseudo
     CDS             2503..3261
                     /locus_tag="VLL03_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079118.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
BASE COUNT          705 a          943 c          966 g          647 t
ORIGIN      
        1 aatgagtatt ggctgcgcgc cgaccccgtg catctgcgcg tcgacagcgg ccagctggtg
       61 cttgccgaca gcagcgcctt agacatcaca caagatgaag caaaccggtt gactcaggcg
      121 ctcaactcgc atttcaataa cgcgggcttg gttttccagg caatgcaccc tgaacgctgg
      181 tacctgcgcc ttgaaaaatt accaaagttg cagactcggg ctttggccga agtggcaggt
      241 aaaaatatag atgagttcct tcccatcggc gcagacagca tttattggca tggcgtgtgc
      301 aatgagatac agatggtgct gcatcatcac gccgtcaatg aagcccgcga agctgtgggt
      361 aatccgccgg tgaacagcgt atggctttgg ggtggcggcc tcctgccgaa aattactggc
      421 aacccgtttg cgcacgtctg gaccaatgag tgcctggcca aaggcctggc gctggcaagc
      481 ggcgccggct tgagcagact gccgcaaaac gcgcaagcat ggctggcgca gccgcatgca
      541 cccggtgttc acttggtggt actggacagc ttgcgcagcg cggcgcaata ccgggacagg
      601 gagcgctggc tggaaaacat taaagaactg gaagcctgct ggtttgcgcc gctgttaacc
      661 gcgctgcagc gtggacaact tgatgaactc gtcctcagca gcggcccctg gtcgttcgct
      721 atagcacgca gcgatttatg gaagctgtgg cgcagaggca aagcgtttgc aaattatgcg
      781 cttggttaac atgctaaaaa tcattccccg cccctgcccc aagccggctt ttgaagcgct
      841 gctgagccag gagatgcacc cggtgctcgc gcgcatttac gccgcccgcg gcattcaaag
      901 cccggtacag ctgaaaagcg aactttcttc gcttgttcct tttgacagat tgaaaaatgc
      961 ggcacagatg gcgcatatcc tggcggatgc catcgcagcg aaaaaacggc ttgtgatcat
     1021 cgccgattac gatgccgacg gcgccaccgc ctgcgcagtg ggcatgcgcg ctttgcgtga
     1081 attcggcgcg ctcgtggatt atctcgttcc caaccgcttc gagtacggtt acggcctgac
     1141 tcctgaaatc gtgcaactgg ccaagcaagt aaaacgcccc gatatattga tcacggtgga
     1201 taacggcatc gccgcagtgg aaggcgtggc cgaagcgagc cgcctcggca tggcaacact
     1261 tatcaccgac catcatttgc cgggtgccga gctgccgcaa gccgcatgca tcgtcaaccc
     1321 caaccagccc gaatgcgaat tccccagcaa aaatctcgcg ggagtgggcg tgatgttcta
     1381 cgtcatgctg gcgctgcgcg ccgagttgcg cgcgctgcac gcattcgaca gcagggccga
     1441 gccgaatctc tccaagctgc tggatctggt ggcgctcggc accgttgccg acgtagtaaa
     1501 gctcgacgac aacaaccgca tcctggtcag ccagggcttg aggcgcatgc gcgaagccaa
     1561 agcgcatgcc ggcgttcagg cattgttccg cgcggccggc cgcgatccct tgcgcgcctc
     1621 gacttatgat ttggcgttca tgttgggccc gcgcctcaat gcggcgggaa gactggatga
     1681 catgtcgctt ggcatcgaat gcctggtaag cgacgatgcg gagtttgccg ccacagctgc
     1741 gcgcaggctc gaccaactca accgcgagcg ccgcgatatc gaggccggca tgcagcaggc
     1801 ggcgctggcc gcgttggagc gcattgaagc gcgcgacagc gtgagcctgt gcttgttcga
     1861 ccccaactgg caccccggtg tggtgggcct gctcgcatcg cgcatcaagg acaaatttca
     1921 ccggccggtc atcgccttcg ctcctgcggg cgacggggga atcaagggct cggggcgggc
     1981 catatccgga ctgcatctgc gcgatgcgct ggatttggtg gcgaaacgtc atcccggttt
     2041 gattctcaag ttcggcggcc atgccgcggc tgcggggctc agcatacgcc agaacgattt
     2101 tgaaagattt tccctggcgt ttgaggaaac ggtagctgcg ctgatctcgc ccgccgatct
     2161 tgagcgcgtg atcgaaaccg acggcacgtt agccatcgcc gacatgcaaa tcgagctggc
     2221 gcgactgctg gaaaaagagg tatggggtca gggttttccg cagcccagct tcagcagcga
     2281 ttttacggtg gaaaaccagc gcgtggtcgg agaaaaacat cttaagctca agctttccct
     2341 gtccggccgc gcttttgatg caatgctgtt ttttcacagc aaaccgctgc cggagcgcat
     2401 cctggcggta tatcggcttg ccgtcaacga atacaatagc gcgcagtcgc tgcagctcaa
     2461 catcgaacat tggcaacccg ctgcatgata aaatgcaccg tcatggaacc cgaacaacta
     2521 aacgctatag ccaaccgact cgccgatctc ggctcccgtt tctcggagct gcggaggtat
     2581 ctttgacttc gatggaaagc aaaaacgtct taatgaagtc gaaaagctcc taacggaccc
     2641 aaacgtttgg agcgatcaga aacgtgccca agaactcggt aaagaaaaaa aatctctgga
     2701 cgccactgta acgggattgg tttcgatagc ctcgaccctg ggtgatgcac gcgagctttt
     2761 cgagatggcg cgtgccgaaa acgacgacgc cacaatggtt ggtgtcgcct ccgacgtcgc
     2821 gaagctggaa caggatgttg ccgacctcga attccggcgc atgttttcca atcccatgga
     2881 tcccaataat tgctttctca tgatccaagc cggctctggc ggcaccgagg cgcaggactg
     2941 ggcggcgatg ctggagcgca tgtatttgcg ctatagtgaa cgacatggct tccgggtgga
     3001 gttgctggaa gaatcggatg gcgaagtcgc gggaattaaa agcgccacga tgaagatcaa
     3061 cggcgattac gcttacgggc ggctgcgcac cgagaccggg gtgcaccggc tggtgcgcaa
     3121 gtcccctttc gattccggca accgccgcca tacctcgttc gccagcgtgt tcgtctatcc
     3181 ggaagtggat gaatcaatcg aggtcgaaat caacccgacg gatttgcgca tcgataccta
     3241 ccgcgcatca ggcgccagat c
//
LOCUS       DATIOW010000174         3882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_889450, whole genome shotgun sequence.
ACCESSION   DATIOW010000174 DATIOW010000000
VERSION     DATIOW010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889450"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /gene="gluQRS"
                     /locus_tag="VLL03_05755"
     CDS             <1..876
                     /gene="gluQRS"
                     /locus_tag="VLL03_05755"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HSS46901.1"
                     /translation="RFAPSPTGPLHFGSLVAAVGSFLEAKAHQGEWLVRIEDLDPPRE
                     EPGAGDEILRELEACGMHWDGEIVYQSARHDAYHAAFHNLQQQGLIYPCACSRLEIAD
                     SAVEGTDGPVYPGTCRNGLPKDRTARSWRLRTDQRAIEFSDALQGCIKQHLEKEIGDY
                     VLYRADRVYAYELAVVVDDAEQGITDVVRGADLLTSTPRQIYLQKLLSLPTPYYLHLP
                     VALDSSGKKLSKQTYAPAVGQGQEMPWLYHALRFLGQNPPPRLAKASLGSFWEWAVLH
                     WDAKKIPQARALQAH"
     gene            889..1551
                     /locus_tag="VLL03_05760"
     CDS             889..1551
                     /locus_tag="VLL03_05760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSS46902.1"
                     /translation="MANKTFGLTDTVYSYLLSASLREPQILSRLRRETAKLPDSRMQI
                     APEQGQFMALLTKLMGAKKCIEIGVFTGYSSLCVALTLPQDGKIIACDVSEEWTQIAR
                     RYWKEAGVTEKIELCLQPALKTLDKLLEENQSGTFDFAFIDADKENYTAYYERCLKLL
                     RRGGLIAIDNTLWSGRVAEPANRETDTVAIREFNSKLHLDQRVALSLLPIADGLTLAL
                     KL"
     gene            complement(1548..2318)
                     /locus_tag="VLL03_05765"
     CDS             complement(1548..2318)
                     /locus_tag="VLL03_05765"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HSS46903.1"
                     /translation="MCQLLGMNCNVPTDICFSFAGFHTRGGATDDHSDGWGIAFFEGV
                     GCRLFLDSKPAARSPVAELVRKYPIRSLNVIAHIRKATQGEVCLQNTHPFMRELWGRY
                     WIFAHNGTLKNFEPQLNETYRPVGTTDSERAFCHILETLRSQFPHGSPQLEALYGAVK
                     GVTQELAQSGMFNYLLCNGEYLFAHCADQLSYVLRRAPFGRAHLKDQDITVDFSRLTT
                     PNDRVAVIATLPLTDNETWTEMAPGELLLFKDGEPQPL"
     gene            2413..2796
                     /locus_tag="VLL03_05770"
     CDS             2413..2796
                     /locus_tag="VLL03_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HSS46904.1"
                     /translation="MQERDDKVRIDKWLWAARFYKTRALAADAVEGGKVQLNGGRVKP
                     SKPLRPGDALEIRQGPYRYSIMVRELSARRGPAAQAARLYAEKEESKINREQLKARLQ
                     MQPLHKGRPTKRARRQLQKFTDYTR"
     gene            complement(2797..2928)
                     /locus_tag="VLL03_05775"
     CDS             complement(2797..2928)
                     /locus_tag="VLL03_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46905.1"
                     /translation="MSQLQKTLMWMVSLVVATGIIYLCFKTYISPASMIDFANTQLC"
     gene            3087..3542
                     /locus_tag="VLL03_05780"
     CDS             3087..3542
                     /locus_tag="VLL03_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46906.1"
                     /translation="MFNKSILAAALAAAFAVAAPAGFAADNDVKQDRQDIRQDTRDIR
                     GDRRDTNGDKRDIREDRRDHNKDVRERNRDRRELMADEREGNTAGIAKDKADLRKDNA
                     DIRKDRKDLHKDRKDLRADKRDLHQDRRDRRHDQRELNKDKKESQENKN"
     gene            complement(3597..>3882)
                     /locus_tag="VLL03_05785"
     CDS             complement(3597..>3882)
                     /locus_tag="VLL03_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HSS46907.1"
                     /translation="TPTTVGNMLERYPGLWVELSSRGDITPDGKLDAGWRGLFLRYPT
                     RFLIGTDTWTASRWNDLPLIAQATRQWLSKLPREVAENIAYRNAEVLFLR"
BASE COUNT          939 a         1133 c         1118 g          692 t
ORIGIN      
        1 cgctttgccc cttcgcccac cgggccgctg catttcggtt cgctggtcgc ggcagtgggc
       61 agttttttgg aagccaaggc gcaccaaggc gaatggctgg tgcgcatcga agatctcgat
      121 ccgccacgcg aagaacccgg cgccggcgac gagattctgc gcgagctcga agcctgcggc
      181 atgcactggg acggcgaaat cgtttaccag agcgcgcgcc acgacgccta tcacgccgca
      241 ttccacaacc tgcagcagca aggtctgatt tacccgtgcg cgtgcagccg cctcgaaatc
      301 gccgattcgg cggtggaagg cacggacgga ccggtctatc ccggaacctg ccgcaacggc
      361 ctgcccaaag accgaacggc gcgctcctgg cggctgcgca ccgaccagcg ggcaattgaa
      421 ttcagcgacg ctctgcaagg atgcatcaag cagcatctgg aaaaagaaat cggcgattac
      481 gtgctttatc gagccgaccg ggtttacgct tatgagctcg cggtagtagt ggacgacgcc
      541 gaacagggaa taaccgatgt cgtgcgcggt gcggatttgc tgacttcgac gccgcggcag
      601 atttacctcc agaaactcct gagtctgccc acgccgtact atctgcatct gccggtggcg
      661 ctcgacagtt ccggcaaaaa actcagcaag caaacttatg cgcccgcagt gggacaggga
      721 caggaaatgc cctggcttta ccacgcattg cgcttcctcg ggcaaaatcc gccgcccagg
      781 ctggccaagg ccagtctcgg cagcttctgg gagtgggcgg tattgcactg ggacgccaaa
      841 aaaattccgc aagcacgcgc attgcaggcg cattagaacc cgctcatcat ggccaacaag
      901 acttttggct taaccgacac cgtttacagc tatctcctgt cggcgtcgct gcgagagccg
      961 caaatcttaa gcagactgcg cagggaaaca gcgaaactgc ccgatagccg catgcaaatt
     1021 gctccggaac aaggccaatt catggcgctg ctcacgaagc tgatgggcgc gaaaaaatgc
     1081 atagaaatcg gcgtcttcac cggctacagt tccctgtgcg tcgccctcac cctcccgcaa
     1141 gatggcaaaa tcatcgcctg cgacgtgagc gaggaatgga cgcaaatcgc gcgccgttac
     1201 tggaaagaag cgggcgtgac ggagaaaatc gagctgtgcc tgcagcccgc gctgaaaaca
     1261 ctggataagc tgcttgagga aaaccagtcg ggaacgtttg atttcgcctt cattgacgcc
     1321 gacaaggaaa actacaccgc ttattacgag cgctgtctga aattgctgcg gcgcggcgga
     1381 ttaatcgcga ttgacaacac cttgtggtcg gggcgcgtgg ccgaaccggc taaccgcgaa
     1441 acggataccg tagcgatacg cgaattcaac agcaaactgc atcttgacca acgggtcgcg
     1501 ctgtcgctgc tgccgattgc cgacgggctg acgctcgcac ttaagctcta aagcggctgt
     1561 ggttcaccgt ctttgaacag gagcagctcg cccggcgcca tctcagtcca ggtttcattg
     1621 tcggtgagcg gcagcgtggc gatcaccgcc accctgtcat tgggcgtggt gagccggctg
     1681 aaatccacgg tgatgtcctg atctttgagg tgcgcgcggc caaacggagc gcgccgcagg
     1741 acgtaggaga gctggtccgc gcagtgcgcg aagagatact cgccgttgca aagcaaataa
     1801 ttaaacattc cgctttgggc gagctcctgc gtgacacctt tcaccgcacc gtagagcgct
     1861 tcaagctgcg gcgagccgtg tggaaattgg ctgcgcaggg tttcaaggat atggcagaag
     1921 gcccgttcgc tgtcggtagt gcctaccggg cgataggttt cattgagctg tggttcaaaa
     1981 tttttcagcg tgccgttgtg ggcaaaaatc cagtaacgtc cccacagttc acgcatgaac
     2041 ggatgggtat tctgcaagca cacctcgccc tgtgtggctt tgcgaatatg ggcgattacg
     2101 ttgagcgagc ggatggggta tttccgcacc agctccgcca ccggggatct tgccgccggc
     2161 ttggaatcga gaaacagccg gcagcccaca ccctcaaaga aagcaatacc ccagccgtcg
     2221 ctgtgatcat cggtggcgcc tccgcgcgta tgaaatcctg cgaaggaaaa gcagatatcg
     2281 gtgggaacgt tgcagttcat ccccagcagc tggcacatcc tgaacctcgg caaaaccggc
     2341 atgcggcaaa ggcaagccgc gctactctag ttataataca aacccggtga aaaataaatt
     2401 ccggcatgag ccatgcaaga acgcgacgac aaagtacgca tcgataaatg gctgtgggcg
     2461 gcgcgtttct acaaaacccg tgcactcgcg gcggacgcgg tcgaaggcgg caaggtgcaa
     2521 ctgaatggcg ggcgggtgaa accctcgaaa ccgctgcgcc cgggcgatgc actggaaatc
     2581 cgccaggggc cttatcgcta cagcatcatg gtgcgggagt tatccgcgcg ccgtggaccc
     2641 gcggcacaag cggcgcggct ttatgcagaa aaggaagaaa gcaagataaa ccgtgagcag
     2701 ctcaaagccc ggctgcagat gcagccgcta cacaaaggcc gccccaccaa gagggcgcgc
     2761 cgccagttgc agaaattcac cgattacacc cgttagtcaa cacaactggg tgttggcaaa
     2821 atcaatcatt gaagccgggc tgatataagt cttgaagcaa agataaataa tgccggtcgc
     2881 caccaccagc gacaccatcc acatcagggt tttctgcaat tgagacatta ttacaccagg
     2941 ctttcatcta atatgtcttt catgttaaaa ctcacggctt gacatggcaa gtgtgccccg
     3001 gttcacaaaa aagccgtcaa cgcgacgggg taaggcgcgt tattcgcttt ggcgccacct
     3061 ttaacccaac acaaggagga ttcacaatgt ttaacaaaag tattctggct gcagccttgg
     3121 ccgccgcttt cgcggtggcc gcgcctgccg gttttgcggc cgataatgac gtgaagcagg
     3181 accggcagga catccgccag gacactcgcg acattcgcgg tgacagacgc gataccaatg
     3241 gggacaagcg cgacatccgc gaagaccggc gcgaccacaa taaagatgtc cgcgagcgca
     3301 accgcgaccg ccgcgagctc atggcggacg agcgcgaagg caacaccgcc gggatcgcaa
     3361 aagacaaagc cgatctcagg aaggataacg ccgatatccg caaggatcgc aaagatctgc
     3421 acaaagaccg gaaagacctg cgcgcggaca agcgcgacct gcatcaagac cgccgcgacc
     3481 gccgtcacga ccagcgcgaa ctgaacaaag acaagaagga atcgcaggaa aacaaaaact
     3541 gaagcaagct taagcgaaac taaaacccgg cctcacgccg ggttttagtt ttcagcttag
     3601 cgcaagaaca acacttccgc gttgcggtag gcgatatttt ccgcaacctc gcgcggcaat
     3661 ttggaaagcc actgccgtgt ggcctgcgca atcaacggca agtcgttcca gcgggaagcg
     3721 gtccaggtat ccgttccgat caggaaccgt gtcgggtaac gcaggaacag tccccgccag
     3781 cctgcatcaa gttttccgtc aggcgtgata tcaccgcgtg aggaaagctc cacccacagg
     3841 ccggggtaac gctccagcat gttgccaacc gttgtgggcg tg
//
LOCUS       DATIOW010000175        14633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1398006, whole genome shotgun sequence.
ACCESSION   DATIOW010000175 DATIOW010000000
VERSION     DATIOW010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14633
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398006"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1691)
                     /locus_tag="VLL03_05790"
     CDS             complement(<1..1691)
                     /locus_tag="VLL03_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HSS46908.1"
                     /translation="MRNANLVSRLQAYSSWRIALSQAVGDYRDWLHEQELDDAQTNLR
                     IQQLRDRLADDKLNVAFVAEISRGKSELINAIFFADYGQRLLPSSAGRTTMCPTELLY
                     DPAREPSIQLLPIETRARHSSTSEYKRFPDEWRNLPLNVNSGEAMVEAIRQVSETRRA
                     PLEEAKSYGLYNEDDADQALLVKEDGTMEIPCWRHAIINFPHPLLKQGLVILDTPGLN
                     AIGAEPELTLNLLPNAHAVLFILAADAGVTKSDIQVWRNHISYLQGQQKGRIIVLNKI
                     DGLWDDLKNPALVENEISGQVRYCSETLGLHASQIFPVSAQKGLVAKVNRDDVLLARS
                     RLPELEQALSDYLIPCKRDIVREKTQSEVEDMVGNARYILEGRLSGVHKQLEELIALR
                     GKNRDVVAHIMQRIKSEKDNFEKSLQRFQALRSVFSQHTNALFACLGMDALQAGVQST
                     RQSMEQSKFSSSLRQAMHQFFNKASENLMSSAKQTVEIRAMMEAMYKKFSAEHGLAEL
                     APAAFSMLKYIKEMEQLRDVYRRHFDTARTMLTHEKATLMNKFFETLASRVVHLYT"
     gene            complement(1752..2567)
                     /gene="mutM"
                     /locus_tag="VLL03_05795"
     CDS             complement(1752..2567)
                     /gene="mutM"
                     /locus_tag="VLL03_05795"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HSS46909.1"
                     /translation="MPELPEVEITRRGLEQRVSGRRISRIAIRNPNLRWKVPPNLPRL
                     LEGFAIHGVARRGKYLLLDCGHGTLIIHLGMSGSLRVLPAETPWQKHDHFDLVLNNGE
                     MVRLHDPRRFGAVLWTESDPLKHPLLKNLGPEPLTNAFNADWIYRQTRSRSTAIKQAL
                     MDSRVVVGVGNIYANEALFRARIHPKTSASRIGPARYEKLVEAVRQTLVLAIKAGGSS
                     LRDFVDCGGNLGCFQMHYLVYDRAGKPCSKCRAVIRQVRLGQRSSYFCPRCQI"
     gene            complement(2578..3417)
                     /locus_tag="VLL03_05800"
     CDS             complement(2578..3417)
                     /locus_tag="VLL03_05800"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HSS46910.1"
                     /translation="MKSRLWLNLILLVAVGALAAVFYFKPKIEAQSGFRLSSLQAGAV
                     KKIVIAKTADSPITVEKHGDHWFITAPIKARADSNLISGVLDLLSLTAKQRLPATDLT
                     RFDLKQPWARLTFDNQEISFGDQHPLTREQFIATEGYVYLIPPQYTRALSLPSNYFLS
                     HRPLTENETPVGFELPGLTLTQKEGKWESSPAIKLTQDQLNRFADEWRHAYCMSSRLV
                     SGQKGPPIKIRLANGSAVNFQLLQKEPDVVLLREDEGIAYQFPTEIGLRLLDPLAAIQ
                     AQK"
     gene            complement(3414..4766)
                     /locus_tag="VLL03_05805"
     CDS             complement(3414..4766)
                     /locus_tag="VLL03_05805"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HSS46911.1"
                     /translation="MQINKKLRIQLLLQNSFFVLLLLAMATLLALLARDHLVQWDVTQ
                     NNRNSLSKASLDTLKQLKGPVTIIAYATQQDPRMGDVRKLIHDFFRPYQLAKPDLRLE
                     FIDPTEQPKLAAGANVQINGEMVVKYGDKSEHLTELKEQTFTNMLMRFARAQERLVMQ
                     LEGHGERKLNGQANHDLGEFGKQLAAKGFQTGTANLAVAQDIPDNTSILVITQPQTDL
                     LPGEIDKLKLYLDKGGNLLWLVDQEPLHGLQALAQTLGIGLTPGVVIDPAAQQLNAPA
                     TWALAAIYGEHPITRNFNLITVFPLARELVIKENTGWKVVSLINVAQRGWVETGKLDQ
                     SVRFDQGKDTPGPVTVALALSRQKDQREQRAVVVGSGTFLANAFLGNGGNLDLGINII
                     NWLARDENLITLQLRAARDSDLRLEKTALTLITAGFLIALPLLFLATGGVIWWRRRKS
                     "
     gene            complement(4772..5515)
                     /locus_tag="VLL03_05810"
     CDS             complement(4772..5515)
                     /locus_tag="VLL03_05810"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSS46912.1"
                     /translation="MIFTLAAKELKALFASPLAWIVIAVLQIIFAWIFLSRVNTFLLV
                     QPQLLQLSNPPGATEFIAMPLFGSVAVVLLMAVPLLSMRLVAEERRNQTMPFLLSAPL
                     SMTEIILGKFLGLLLFLAAVIGLAALMAVSLGLGTQPDYGLIIGNVLGLLLIVACYAS
                     LGLFFSCLTAQPVVAAIATLGALLGLWVIDAAAGAPDSWLHWVSLLKHFESFNRGLID
                     TADIAFLALFTTAFLALAIRRLDADRLRG"
     gene            complement(5517..6461)
                     /locus_tag="VLL03_05815"
     CDS             complement(5517..6461)
                     /locus_tag="VLL03_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSS46913.1"
                     /translation="MSEEISTTTLSAQALSRSFGPRPAVSEVTLELKKGEVLGFLGPN
                     GAGKTTTMRMLSGNLAPSSGSVRICGIDLFEKPKAAKALIGYLPEIPPLYRDLTVDEF
                     LKLAARLHRVAKKQIQGTIARAKERCGLNDVGRQLIGGLSRGYRQRVGIAQAIVHTPE
                     VVILDEPTVGLDPIQIREIRTLIRELGGAHSVILSTHILPEVETVCDRVHILHQGKGV
                     YSGGVASLMQMRAGHLLQVGLRNPPPVAELERIAGVTAVEKSGAGQFQVRFSPETDPT
                     DALVTTAVEKNWRLHQLNRQQTSLEDVFVQLTQKEEDT"
     gene            6632..8386
                     /locus_tag="VLL03_05820"
     CDS             6632..8386
                     /locus_tag="VLL03_05820"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS46914.1"
                     /translation="MKQKFLILALSAWFCACAQQPQKPILVTTEASPEPQAEDQLEQP
                     DSATKPANLPKQELSDSVLYEFLLGEIAGQRGQLNIAAKAYLDIAKATLDPRIAQRAT
                     EIALYARQPEAALQAARIWVETDPDSARARQTLVALLVGARKLEEARPHIEKLLAAEG
                     ENIGQGFLQLNGLLTKTADKNAALNLVRDLAKPHLRVPEVHLALSQAAWNAAQYDVAL
                     SEVRKALELRSDWELAALFQGQILQRDSNHLAIDYLHGYLKSHPKAKDVRLSYARLLV
                     AEKNYPQARKEFQQLMADFPKNAEVSLAVGLLSMQLNDYDAAEANFKQALSLNYKDQD
                     SVRFYLGQVYEERKRYDEAQRWYGSVGPGELYISAHTRYANMLGKQGKLAQAREYLHK
                     LEPQDLQQRMQIVLAEAQLLRDAGRYQEAFDLLGRTLEKNPNYPDLLYDYAMAAEKLN
                     RLDILEDNLRKLIKLKPDYAHAYNALGYTLADRNERLSEAVEFIRQALQLAPEDPFIM
                     DSMGWVQYRLGNFKEGADYLQRAFANRPDPEIAAHLGEVLWAQGRREEAQQIWRTSLK
                     DNPDNETLQNVMKKFAGK"
     gene            8436..8999
                     /gene="lolB"
                     /locus_tag="VLL03_05825"
     CDS             8436..8999
                     /gene="lolB"
                     /locus_tag="VLL03_05825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HSS46915.1"
                     /translation="MVCGCVSIPSKAPSHPQSRAQSLHSAQQHGFELSGRVAVKYNEK
                     SFSGGMRWTHRADTDEIWILAPLGRGVARIVRDDAGITLTTDDQKIYRARDTESLTRE
                     VLGWSLPLAGLQYWVLGVAAPQSPAEAQSDNDGRIERLNQDQWEIAYLRYKPEPESGM
                     PGRIQLRRGDLNISLIVDDWTIASNSR"
     gene            8996..9832
                     /gene="ispE"
                     /locus_tag="VLL03_05830"
     CDS             8996..9832
                     /gene="ispE"
                     /locus_tag="VLL03_05830"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013207048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HSS46916.1"
                     /translation="MNLATFPAPAKLNLFLHVVGRRPDGYHLLQSVFRFVDFSDSLGF
                     RVMEDGAIRRVRELPGVPAEQDLCMRAAKLLQQASGTPLGVEITLEKKLPLGGGLGGG
                     SSDAATTLIALNRLWKLNFSRALLQELGLKLGADVPVFVFGQSAFAEGVGEVLQPINL
                     PPAWYVVLVPKVNISTTEIFADSTLTRNMKPIKINSFFIGQGHNDLEPVVCRKYPEVA
                     QHLAWLKQFGPARMTGSGGCVFAEYAREEEARAVLARLPREMSGFVARGLDRHPMHDL
                     AF"
     gene            9847..9921
                     /locus_tag="VLL03_05835"
     tRNA            9847..9921
                     /locus_tag="VLL03_05835"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9879..9881,aa:Gln,seq:ttg)
     gene            9972..10922
                     /locus_tag="VLL03_05840"
     CDS             9972..10922
                     /locus_tag="VLL03_05840"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HSS46917.1"
                     /translation="MAYDSLMVFTGNANPKLAEQVVKHLNIHLGRATVGRFSDGEVMV
                     EILENVRGKDVFVLQSTCSPPNESLIELLVMVDALKRASAGRITAAIPYFGYARQDRR
                     PRSARVPITAKMVANMLTSVGVDRLLIMDLHSEQIQGFFDIAVDNIYAMPLLLGDIWK
                     HDHQNLVVVSPDVGGVVRARAAATRLEADLAIIDKRRPKPNVAKVMNIIGEVKGRTCV
                     IMDDLVDTANTLCEAAEALKREGAEKVLAYCTHPVLSGKAVERIENSVLDELVVTNTI
                     PLTEAARKCKRIRCLCVAELLGETMLRISNEDSVSSLFME"
     gene            11050..11691
                     /locus_tag="VLL03_05845"
                     /pseudo
     CDS             11050..11691
                     /locus_tag="VLL03_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419343.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
     gene            11893..12468
                     /gene="pth"
                     /locus_tag="VLL03_05850"
     CDS             11893..12468
                     /gene="pth"
                     /locus_tag="VLL03_05850"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HSS46918.1"
                     /translation="MKLVVGLGNPGKEHTANRHNVGFRWLERLASAERVMLKAEARFH
                     ALLGQLDREGEECWLMQPQTFVNASGRAVRALAEFYKIPATEILVVHDELDLPPGVAR
                     LKQGGGVSGHNGLKDIAAHLGTQDFWRLRIGIGHPGEKALVVDYVLDRPPAGEKKLIE
                     QAIHQSLEIWPLIAENKHQAAMLKLHTKQIQ"
     gene            12465..13559
                     /gene="ychF"
                     /locus_tag="VLL03_05855"
     CDS             12465..13559
                     /gene="ychF"
                     /locus_tag="VLL03_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HSS46919.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGIV
                     ELPDARLQALAGIAKPQKVQPALVEFVDIAGLVAGASKGEGLGNQFLAHIRETDGIVH
                     VVRCFEKENVVHVTGKIDPIADIEIVNTELALADLATVEKQYARYAKAAKAGGDKEAQ
                     RIIAVLDKIQPVLNQAKPARSVRLAKEELAVLKPLFLLTAKPTMYVANVAEDGFENNP
                     LLDQVRVHAKAENAPVVAICAELEAEIADLPLEEKQVFLADMGLKEPGLNRLIRVAFD
                     LLGLHTFFTTGPREARAWTIHKGDTALDAAAVIHTDFARGFIRAEVIAYADYVACKGE
                     HGAREAGKMRLEGKDYIVRDGDVIYFRFNV"
     gene            14011..14352
                     /locus_tag="VLL03_05860"
     CDS             14011..14352
                     /locus_tag="VLL03_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46920.1"
                     /translation="MLALVEALPVESIRQVLCTPGINLLQELDPPLETVAVNEHERLK
                     PDLARRVLETVRVSRESNPPGALVALGEIVKSIRNKRAHGFKSRNNKRDAEILRAARG
                     ILTGLCELVAA"
BASE COUNT         3115 a         4042 c         4434 g         3042 t
ORIGIN      
        1 gtgtagaggt gcaccacgcg gctcgccaac gtttcgaaaa acttgttcat cagcgtggct
       61 ttctcatggg tgagcatggt gcgggcggtg tcaaaatggc gccggtacac atcgcgcagc
      121 tgttccattt ctttaatgta cttcagcatc gagaaggccg ccggtgcgag ttcggcgagc
      181 ccgtgctcgg cgctgaattt cttgtacatc gcttccatca tggcgcggat ttccacggtc
      241 tgcttggcgg agctcatcag gttttcgctg gctttgttga aaaactggtg catggcctgc
      301 cgcaggctcg agctgaactt gctctgctcc attgactgac gggtactctg tacccccgct
      361 tgcagcgcgt ccatgccgag gcaggcgaag agcgcgttgg tgtgctgcga aaacacgctg
      421 cgtagcgcct ggaagcgttg caggctcttt tcgaagttgt ctttttcgct tttgatccgc
      481 tgcattatgt gcgcgactac gtcccggttc ttgccgcgca gggctatgag ttcttcaagc
      541 tgtttgtgca cgccggaaag ccggccttcg agaatatacc tggcgttgcc caccatatcc
      601 tcgacttcgc tctgggtttt ttctcgcacg atgtcccgtt tgcacggaat caggtagtcg
      661 gaaagcgcct gctcaagttc cggcagacgg cttctggcga gcagcacatc gtccctgttg
      721 accttggcca ccaggccttt ctgcgccgac accgggaaga tttgcgaagc gtgcaggccc
      781 agcgtctcgg agcagtatcg cacctgcccg gagatctcgt tttccaccaa tgccgggttt
      841 ttcaggtcat cccacaagcc gtcaatcttg ttaagcacga tgatgcggcc tttctgctgg
      901 ccttgtagat agctgatgtg gttgcgccac acctggatgt cggatttggt gacgccggca
      961 tcggccgcca gaatgaacag caccgcgtgc gcgttcggca aaagattgag cgtcagctcc
     1021 ggttcggcgc cgatggcgtt cagacccggc gtatccagaa tcaccaggcc ctgtttcaaa
     1081 agcggatgcg gaaaattgat gatcgcgtgc cgccagcagg gaatttccat ggtgccgtct
     1141 tcctttacca gcagcgcctg gtcggcatcg tcctcgttat acaggccgta gctcttggct
     1201 tcttccagcg gcgcgcgccg ggtttcgctc acctgccgga ttgcttccac cattgcttca
     1261 cccgaattca cattcagcgg caggttgcgc cattcgtcgg gaaaacgctt atattcgctg
     1321 gtgctggaat gcctagcacg ggtttcgatc ggcagcagct ggatgctcgg ctcgcgcgcc
     1381 gggtcgtaca gcagttcagt cgggcacatg gtggtgcgtc cggcgctgga aggaagcagc
     1441 ctttggccgt aatcggcgaa aaaaatcgcg ttgatgagtt ccgacttgcc gcgcgagatt
     1501 tcggcgacaa aggcgacgtt tagcttatcg tcggccaggc ggtcgcgcaa ttgctggatg
     1561 cgtaaattgg tctgcgcgtc gtcgagctcc tgctcgtgca gccagtcgcg gtaatcgccg
     1621 acggcctgcg acagcgcaat gcgccagctg ctgtaggcct ggagtcgcga aacgaggtta
     1681 gcgttcctca tgagtttagg cgccatcaaa aacaagggta agttctaact ctagcataaa
     1741 caagatttag cttagatttg gcaacgcgga caaaagtagc tggagcgctg gccgagccgc
     1801 acttgacgga tcacagcccg acatttgctg cagggtttgc ctgcgcggtc gtaaaccaga
     1861 tagtgcatct ggaagcagcc gaggttgccg ccgcagtcaa cgaaatcgcg caagctactg
     1921 ccgcctgcct tgatggcgag caccagcgtc tggcgcacgg cctcgaccag tttttcgtaa
     1981 cgcgcagggc cgatgcgcga agcggaagtc ttggggtgga tgcgcgcgcg aaacagcgct
     2041 tcattggcgt agatattacc cacgcccacc accacgcggc tgtccatcag tgcttgcttg
     2101 atggctgtgc tgcggctgcg cgtttgccgg taaatccagt cggcgttaaa cgcgttggtt
     2161 aacggctccg ggccgaggtt tttgagcaag gggtgcttca ggggatcgct ctcggtccac
     2221 agcaccgcgc caaagcggcg cggatcgtgc agccgcacca tctcgccatt attcaaaacc
     2281 agatcgaagt gatcatgttt ctgccagggc gtctccgcgg gcaggactcg caggctgccc
     2341 gacatgccga gatgaatgat cagcgtgccg tgcccgcaat ccagcagcaa atacttgccg
     2401 cgccgggcca cgccatggat ggcgaagccc tccagcaggc gcggcaaatt tggcggcacc
     2461 ttccagcgca aattcggatt acgaatggca atcctggaga tgcgccggcc gcttacacgc
     2521 tgttccaatc cgcggcgggt gatttcgact tcaggcagtt ccggcatgct ggggggctta
     2581 tttttgcgct tggattgcgg cgagcggatc aagcaggcgt aacccgattt ccgttgggaa
     2641 ctgataggcg atgccttcat cttcgcgcag cagcaccacg tccggctctt tttgcagtaa
     2701 ctgaaagtta acggcagaac cgttggcgag tcggattttg atgggcgggc ctttctggcc
     2761 gctcaccagc cggctgctca tgcagtaagc atgtcgccac tcgtcagcga agcggttaag
     2821 ctgatcctgg gtaagcttga ttgcaggcga gctttcccac ttgccttctt tttgcgtcag
     2881 cgtcagtccc ggcaactcga aacccaccgg tgtttcattc tcggtgagcg gccggtggct
     2941 caggaaataa ttgctgggca ggctcagggc acgggtgtat tgcggcggga tgagatagac
     3001 gtagccttca gtggcgataa attgttcgcg ggtgagcgga tgctgatccc caaagctgat
     3061 ttcctggttg tcgaaagtaa gcctggccca cggctgtttc agatcaaaac gcgtgagatc
     3121 agttgccggc aagcgttgct tggcggtcag ggacagcaga tcgagcacgc cggagatcaa
     3181 attgctgtcg gcgcgcgcct tgatcggcgc ggtgatgaac cagtggtcgc cgtgcttttc
     3241 cacggtaatc ggcgaatcgg cggttttggc aatcacgatt ttcttgaccg cgcccgcctg
     3301 caaactggaa agcctgaatc cgctttgcgc ttcgattttc ggcttgaagt aaaacactgc
     3361 ggccagagcg ccgactgcga ctagcaggat aaggttaagc caaagccgcg atttcatgat
     3421 ttcctgcgac gccaccagat gacgccgccc gttgccagga acagcagcgg caaggcgatg
     3481 agaaagcctg cggtaatcag tgtgagcgcg gttttctcca ggcggagatc gctgtcacgg
     3541 gcggcgcgca gctggagggt gatcagattc tcgtcccgtg ccagccagtt gatgatgtta
     3601 atgccgaggt cgagattgcc gccgttaccg agaaaagcgt tggcgagaaa cgtgcccgag
     3661 cccaccacca ccgcacgctg ttcacgttgg tctttttgcc ggctgagggc gagcgccacg
     3721 gtcaccggtc ccggcgtgtc ttttccctgg tcgaagcgca cgctctgatc gagtttgccg
     3781 gtttccaccc aaccgcgctg cgccacatta atcaacgaca ccactttcca gcccgtgttt
     3841 tccttaatca ccagttcgcg cgccaaaggg aacaccgtaa tcaggttgaa attgcgcgta
     3901 atcgggtgct cgccgtagat cgcggccagc gcccaggtag ccggcgcgtt gagttgctgg
     3961 gcggcgggat caatgaccac cccgggagta agcccgatgc ccagggtttg cgccagcgcc
     4021 tgcaggccat gcagtggttc ctggtcgacc agccacaaca ggttaccacc cttgtcgaga
     4081 tagagcttga gcttgtcgat ttcacccggc aacagatcag tctgcggctg agtgatgacc
     4141 agtatgctgg tattgtcggg aatgtcctgc gccaccgcca gatttgccgt gccggtctgg
     4201 aaacccttgg cagccagttg ttttccgaac tcgcccaaat catggttggc ttggccgttc
     4261 aactttctct cgccatggcc ctcgagttgc atcaccagcc gctcctgcgc gcgggcgaag
     4321 cgcatcagca tgttggtgaa ggtttgttcc ttgagctcgg tgaggtgttc agatttgtcg
     4381 ccgtacttca ccaccatctc gccgtttatt tgcacgttgg cgccggcggc gagtttcggc
     4441 tgctcggtgg ggtcaatgaa ttccagcctg aggtcgggtt tggccaattg gtacgggcgg
     4501 aagaagtcgt gaatcagttt gcgcacatcg cccatgcgcg ggtcctgctg cgtggcataa
     4561 gcgattatgg tcaccggccc cttaagctgc ttgagtgtat cgagactcgc cttggacagc
     4621 gaattgcggt tgttttgcgt cacgtcccac tgtacaagat ggtcgcgagc gagcagcgca
     4681 agcagcgttg ccatcgccag cagcagcagc acgaaaaaac tgttttgcag cagtaactgg
     4741 atacgcagtt ttttgttaat ctgcatcgtt gtcagccgcg caaacggtcg gcgtcaagac
     4801 gtcttatcgc cagcgccaaa aaggcagtgg tgaaaagcgc aaggaatgcg atatccgcgg
     4861 tgtcgatcaa accacgattg aaactttcaa aatgcttgag cagcgacacc cagtgcagcc
     4921 agctgtcagg cgcgccggcc gccgcatcaa ttacccacaa accgagcagc gcgcccagcg
     4981 tggcgatggc ggcgaccacc ggctgcgcgg tgaggcaaga gaagaaaagc cccagcgacg
     5041 cataacatgc cacgatgaga agcagcccca gcacattgcc gattatcagg ccgtaatcgg
     5101 gctgggtgcc gaggccgagc gacaccgcca ttagcgcggc aagtccgatt accgcggcga
     5161 ggaacagcag cagaccgaga aattttccaa ggatgatttc ggtcatcgaa agcggcgccg
     5221 agagtagaaa cggcatggtc tggttgcgcc gctcttccgc caccagccgc atcgacaaaa
     5281 gcggcaccgc catcaacagc accacggcga cactgccaaa cagcggcatg gcgataaatt
     5341 cggtggcccc cggagggttg ctgagttgca gtaattgcgg ttgcaccagc aaaaaagtgt
     5401 tgacacgcga caggaagatc caggcgaaaa tgatttgtaa tactgctatg acaatccacg
     5461 ccagtggcga ggcgaacagc gcttttagtt ccttagcggc aagggtgaag atcatctcag
     5521 gtgtcttctt ccttttgcgt gagttgcacg aacacgtctt ccagactcgt ctgctgccga
     5581 ttcaattgat gcaacctcca gtttttttcc acggcggttg ttaccaacgc atcggtggga
     5641 tcggtttccg ggctgaaccg cacctggaat tgaccggcgc cgcttttttc aaccgcggtc
     5701 acgccggcaa tacgttccag ctcggcaacc ggcggaggat tgcgcaaacc cacctgcagc
     5761 aggtgaccgg cccgcatttg catgaggctg gctacgccac cgctatagac gcctttgccc
     5821 tgatgcagga tatgcacgcg gtcgcacacg gtctccactt cgggcaggat gtgggtggag
     5881 agaatcacgc tgtgtgctcc gccgagttcg cgaatcaaag tgcggatttc gcggatttgg
     5941 atcgggtcga gccccaccgt cggctcatcg agtatcacca cttccggagt atgcacgatg
     6001 gcctgggcga tgcccacccg ctgccggtag ccgcgcgaca ggccgccgat aagctgcctg
     6061 cccacatcgt tcagcccgca acgctccttg gcgcgggcga tggtgccctg gatttgcttt
     6121 ttggcaacgc ggtgcagcct ggcggcaagc ttgagaaatt catcgacagt caggtcgcgg
     6181 tatagcggcg ggatttccgg caaataaccg atcaacgcct tggcggcctt gggtttctcg
     6241 aaaagatcaa tgccgcaaat cctgacactg ccgctggagg gcgcgaggtt gccgctgagc
     6301 atgcgcatgg tggtggtttt gccggcgcca ttgggaccca gaaatcccag cacctcgcct
     6361 tttttaagct ccagcgtcac ttcggatacg gcggggcgcg ggccaaagga acgcgacaag
     6421 gcctgcgcag agagggtggt ggttgaaatt tcttcgctca ttcggcaata aactcgagca
     6481 actcaagcaa aacccgcgcg cgagaacaaa ttgcacacag cgcaggtctc ttgtcgcgag
     6541 tgcaaaatat acctaatatg tcaacattat taaagaactt cggcgtaatc gaagcttcta
     6601 agtttttgat tatccgtcta ggatacctgc aatgaagcaa aaattcctta ttctcgcttt
     6661 gtcggcatgg ttttgcgcgt gcgcccagca gccgcaaaaa ccgatcctcg ttaccaccga
     6721 ggcgagcccc gagccgcaag cagaggacca gctggaacag ccggattccg ctaccaagcc
     6781 ggccaatctt cccaagcagg aattgagcga cagcgtgctt tatgaattcc tgctgggcga
     6841 gattgccggg cagcgcgggc agctgaatat tgccgccaag gcttatctcg atattgccaa
     6901 ggccacgctc gacccgcgca tcgcgcagcg cgccactgaa atcgcgctct acgcgcgcca
     6961 gccggaagcg gcgttgcagg cggcgcgtat ttgggtggaa accgaccccg attccgcccg
     7021 ggcgcggcag accctggtgg cgctgctggt cggtgcacgc aagctggagg aggcgcgtcc
     7081 gcatatcgaa aagcttttgg ccgcagaagg cgaaaacatc ggccagggct ttctgcaatt
     7141 gaacgggctg ctgaccaaga ccgccgacaa gaacgcggcg cttaatttag tgcgcgacct
     7201 ggccaaaccg catctgcggg tacccgaggt gcatctggcg ctaagccagg cggcgtggaa
     7261 tgcggcgcaa tacgatgtgg cgctcagcga agtgcgcaag gcgctggagc tgcgctcaga
     7321 ttgggaactc gccgctttat ttcagggcca gattctgcag cgcgactcga accaccttgc
     7381 cattgactac ctgcacggtt atttaaaaag ccatcccaag gccaaggatg tgcgcttaag
     7441 ttacgctcgg ctcctggtcg cggaaaaaaa ctacccccag gcccgcaagg aatttcaaca
     7501 gctgatggcc gatttcccca agaacgccga agtgtcgctg gcggtggggc tactctccat
     7561 gcagctcaat gattacgatg ccgcggaggc gaatttcaag caggcgttga gcctcaatta
     7621 caaggaccag gacagcgtgc gcttttacct gggccaggtg tacgaggagc gcaagcgcta
     7681 cgacgaggcg cagcgatggt atggcagcgt cggccccgga gagctgtata tttcggccca
     7741 cacccgctac gccaacatgc tgggcaagca gggcaagctg gcccaggcgc gcgaatacct
     7801 gcacaagctg gagccgcagg atttgcagca aagaatgcaa atcgtactcg ccgaagcgca
     7861 actgctgcgc gacgcggggc gctaccagga ggcgtttgat ttgttgggac ggacgctgga
     7921 aaaaaatccg aattacccgg atttgctcta cgactacgcg atggctgcgg aaaaattgaa
     7981 tcgcctcgat atactcgaag acaatttgcg caaactgatc aagctcaagc ccgattacgc
     8041 gcatgcctac aacgcattgg gctacacgct ggcggaccgc aacgaacgcc tgagcgaggc
     8101 ggtggagttc atccggcagg cgctgcagct cgcgccggaa gacccgttca tcatggacag
     8161 catgggctgg gtgcaatacc gcctcggcaa tttcaaggaa ggggcggatt atttgcagcg
     8221 cgcgttcgcc aaccgccccg atcccgaaat cgcggcgcac ctgggtgaag tgctgtgggc
     8281 gcaaggccgg cgtgaggaag cgcagcagat ctggcgaact tcgctcaagg acaatccgga
     8341 caacgaaacg ttgcagaatg tgatgaagaa attcgccggc aagtgatgtt aaggaacctc
     8401 tgattaagtc ccgcgccgtt gcgatacttg cgctgctggt ttgcggctgc gtttccattc
     8461 cgagtaaagc gccgtcccat ccgcaatcgc gggcgcaaag cctgcacagc gcgcagcagc
     8521 acggcttcga gttgtccggg cgggtggcgg tcaaatacaa tgaaaaaagc ttttccggcg
     8581 gcatgcgttg gacgcaccgc gcggatacgg acgaaatctg gatccttgcg ccgctgggga
     8641 gaggggtcgc gcgcatcgtg cgcgatgacg cgggcatcac gcttaccacc gacgaccaga
     8701 aaatctatcg cgcgcgcgac accgaaagcc tgacgcgcga agtgctgggc tggagtttgc
     8761 cgcttgcggg tctccagtat tgggtgctgg gcgtggccgc gccgcaaagt ccagccgagg
     8821 cgcaaagcga caatgatggg cggattgagc gtctcaatca ggaccaatgg gaaatcgcct
     8881 atctgcgcta taagccggaa ccggaaagcg gcatgccggg acgcattcaa ttgcgccgcg
     8941 gcgatttgaa catcagcctg attgttgacg actggaccat cgcttcaaat tcacgatgaa
     9001 tttagccacc tttcccgctc cggccaaact caacttgttt ctgcacgtcg tcggacgccg
     9061 acccgacggc taccatttat tgcaaagcgt gttccgcttt gttgatttca gcgacagcct
     9121 gggcttccgg gttatggagg acggcgcaat ccgccgcgtc cgggagcttc ccggtgtgcc
     9181 ggcggaacag gatctctgca tgcgcgcggc gaaattgctg caacaagcaa gcggcacgcc
     9241 cctgggcgtg gaaatcacgt tggaaaaaaa actgccgctg ggcggcggtt tgggcggcgg
     9301 cagttccgat gccgccacca cgctgatcgc gctcaaccgt ttgtggaaat tgaatttcag
     9361 ccgcgctctg ctgcaagagc tgggactcaa gcttggcgcg gatgtgccgg tgtttgtgtt
     9421 tggccaaagc gcgttcgccg aaggagtggg cgaggtgttg cagcccatca acctgccgcc
     9481 ggcgtggtat gtggtgttgg tgccgaaagt caacatttca accaccgaga ttttcgcgga
     9541 ttctacattg acacgaaaca tgaaacctat caaaataaac agctttttta tagggcaggg
     9601 tcataacgac ctggaacctg tagtctgccg caaatacccg gaggtggcgc agcatctggc
     9661 ctggttgaag cagttcggcc cggcccgcat gaccggctcg gggggatgtg tgtttgcgga
     9721 gtatgcgcgg gaagaggagg cgcgcgccgt gctggcgcgg ctgcctcgcg aaatgagcgg
     9781 ttttgtggcg cggggccttg accggcatcc tatgcatgat cttgcctttt agcgagaagc
     9841 gagacttggg gagtcgccaa gtggtaaggc accggatttt gattccggca ttcgtaggtt
     9901 cgatccctac ctccccagcc aggtttagag tcttttaggt gaagcagcct caagcgatcg
     9961 ggggaagcgg cgtggcttat gacagtttga tggtgttcac cggcaatgcc aaccccaagc
    10021 tcgccgagca ggtggtgaaa catctgaaca tccatctcgg gcgggccacc gtcggccgct
    10081 tcagcgacgg cgaagtgatg gtggaaattc tggaaaacgt gcgcggcaaa gacgttttcg
    10141 tgttgcaatc cacttgctcg ccgcccaacg aaagcctgat tgaattgctg gtgatggtgg
    10201 atgcgctgaa acgtgcttcc gccggacgca tcacggcggc gattccgtat ttcggctatg
    10261 cgcgccagga ccgcaggccg cgctcggcgc gggtgccgat caccgccaag atggtggcaa
    10321 acatgctgac cagcgtgggc gtggaccggc tattgattat ggatttgcac tccgaacaaa
    10381 tccagggttt tttcgacatt gcggtcgaca atatttacgc catgccgctt ttgctgggcg
    10441 atatctggaa acatgatcac cagaatttag tggtggtctc gcccgatgtt ggcggagtgg
    10501 tgcgcgcgcg cgccgccgcg acgcgcctgg aagcagattt ggcgatcatc gacaagcgcc
    10561 gtcccaagcc caacgtggcc aaggtgatga acatcatcgg cgaagtcaaa ggccgcacct
    10621 gtgtcattat ggatgacctg gtggataccg cgaacacgct gtgcgaggcg gcagaggcgc
    10681 tgaaacgcga aggtgcggaa aaagtgctgg cgtattgcac gcacccggtg ttgtcgggaa
    10741 aagcggtcga gcgcatcgag aattcggtgc tggatgaact ggtggtgacc aacaccatcc
    10801 cgctgactga agccgcaagg aaatgcaaac gcattcgctg cctgtgcgtc gccgaactgc
    10861 tgggcgagac gatgctaaga atcagcaatg aggattcggt aagttcgctg tttatggaat
    10921 gagttttaaa ccgagcgtcg agaatttttt gttctcccgg cgttcctgga gtcttggcgg
    10981 ttaaggtaag gttttatcaa cggtgttgcc tggtcgcggg caatacattt tttggagtga
    11041 atcatgcaaa tcgaagtcac tgcaacatcc cgctcggtgc aaggtacggg tgcgagccgc
    11101 cgcctgcgcc gtgacggcaa ggtgccgggc gttctctatg ggggaaaaga accgacgcag
    11161 gtcattgagc tggaccacaa cgcgctacat ctcctgctca aacaggaagc atttcacgcc
    11221 tcgattctca gcatgaaggt tgacggcaag aaacagcggg tgctgctgcg caatgtgcat
    11281 atgcatccct ggaagccgat ggtgatgcat gtggatttcc agcgcgtggc caaggaccag
    11341 aagattcaca tcaaggtgcc gctgcacttc atcaatgccg agatcgcgcc gggcgtgaaa
    11401 gtcgacgccg gtatcgtcag ccacgtgatg aacgaagtgg aagtttcctg cctgccggac
    11461 aatctgccgg aatttatcga ggttgatctg gcaagcctcg agctcgggga cgcggtgcac
    11521 ctttccgacc tgaaattccc ccaggacgtc gaatccaccg ccctggtgcg cggcagcgat
    11581 tcggtggtgg cgaccgtgca agtgccgaag gtgatcgtgg tcgaggaagc gccagtggca
    11641 cctgcggctg aagaagctgc ggcggctgaa ggcgcggcaa ctgaagcggc ggcggccacc
    11701 ggcgagaaga aagaaggcga gaaggcggcc gacaagccgg aagccggcaa gaaagaaggc
    11761 ggcaaagcgc ccgaaaccgg caagaaagaa agcggcaagg cagagaagaa ataattcttt
    11821 ttgtttgatc ttgcaaagcc cgccatgctt tattaacgaa ctggcgggct ttgtttttga
    11881 aaagaaggaa aaatgaagct ggtcgtcggt ctgggtaatc ccggcaagga acatacggcc
    11941 aaccggcaca acgtcggttt ccgttggctt gaacgcctgg catcagcaga gcgggtcatg
    12001 ctcaaggcgg aggcgcgctt tcatgcgctt ctgggacagc tcgaccgcga aggcgaagaa
    12061 tgctggctga tgcagcctca gacgttcgtg aatgccagcg gccgcgccgt ccgcgcgctg
    12121 gccgagtttt acaaaattcc cgctacagag attttggtgg tgcatgacga actcgatttg
    12181 ccgccgggcg tggcgcgcct gaagcagggc ggtggcgttt ccggtcataa tggtctcaag
    12241 gacatcgccg cgcatttggg cacgcaggat ttctggagat tgcgcatcgg cattgggcat
    12301 ccgggagaaa aagcgctggt tgttgattac gtgctcgatc gccctcctgc cggagaaaaa
    12361 aaactcatcg agcaggccat tcaccaaagc cttgaaatct ggccgctgat tgcggaaaac
    12421 aagcaccaag cggcaatgct caaactgcac acaaagcaaa tccaatgagt cttaagtgcg
    12481 ggatcgtggg gctgcccaac gtcggcaagt ccactttgtt caacgcgctg accaaggcgg
    12541 gaatcgccgc cgagaattat cctttctgca ccatcgagcc caacgtcggc atcgtcgagt
    12601 tgcccgatgc gcgcttgcag gcgctggccg gcatcgcgaa accccagaaa gtccagcccg
    12661 cgctagtcga gttcgtggat attgcgggtt tggtggccgg cgcatccaag ggcgaagggt
    12721 tgggcaacca gtttctcgcc cacatccgcg aaaccgacgg catcgtgcac gtggtgcgct
    12781 gcttcgaaaa agagaacgtg gtgcacgtta ccggcaagat cgatccgatt gctgacattg
    12841 aaatcgtcaa caccgaactg gcgctcgccg atctggcaac ggtggaaaag cagtatgcgc
    12901 gctacgccaa ggcggcgaag gcgggcggcg acaaggaagc acagcgcatc attgccgtgc
    12961 tcgacaaaat tcagcccgtg cttaatcagg caaagccggc acgcagcgtg cgacttgcca
    13021 aggaggagct ggcagttctg aaaccgctgt ttctattgac cgcgaagccc acgatgtacg
    13081 tggccaacgt cgcggaagat ggatttgaaa acaatccgct gcttgaccag gtgcgggtgc
    13141 atgcaaaagc ggagaatgcc ccggtggtgg cgatttgcgc ggagctggaa gcggagatcg
    13201 ccgatttgcc gctggaagag aagcaggtgt tcctcgccga tatgggactg aaagaacccg
    13261 ggcttaaccg cttaattcgc gtcgcgtttg atttgctggg cttgcacact tttttcacca
    13321 ccggccccag ggaagcgcgc gcctggacca tccacaaggg tgataccgcg ctggatgcgg
    13381 cggcggtgat acacaccgat ttcgcaagag gtttcatccg cgccgaggtg attgcctacg
    13441 cggattacgt ggcttgcaaa ggcgagcatg gcgcgcgcga agcgggaaaa atgcggctgg
    13501 aaggtaagga ttacattgtg agggacggag acgtgattta cttccggttt aatgtttagc
    13561 aaaactactg cgcgtccgat ctgcggtgtt gcgcgctcgc tcaaggctcg ccgtactctc
    13621 tttgtactgt ctcgcctttt gctcgcccgc gcctagcatc tcggcctgct cgcaacgttt
    13681 tgcatcgagc tgctaaggct tacatgcata attaaagacc gggctggcca ggcattatga
    13741 tgttgggtaa tattgctttc gcatgatatc ccctattttt ggggcaatgt tttaccccat
    13801 aaaggggtcg attttaattt aatcctcggt gaagcaagag ttgaagaata tggcggtgga
    13861 ggcaaggtcg ttggatgctc agtcaagaca ggagatccga gcggaactag cggcttgtga
    13921 agcgttcggt gactacgagg ccgtcgatgt actttggctc actctcctca aggccgccaa
    13981 tcggttgaca ggcaagtctg aacacggcgc atgcttgcac ttgttgaagc cctccccgtc
    14041 gaatcaatca gacaggttct ttgcactcca gggattaacc tgctccagga acttgatccg
    14101 ccgctcgaaa ccgtagcagt gaatgagcat gagcgcctga agccggattt ggcacgcagg
    14161 gtccttgaaa cagttcgcgt gtctcgggaa tcgaatcccc caggggcttt ggttgcgctt
    14221 ggcgagatcg taaagtcaat ccgcaacaaa cgcgcgcatg gcttcaaatc aaggaataat
    14281 aaacgcgacg cagagattct ccgcgcggct cgcggaattc tgacggggtt gtgcgagctt
    14341 gtagcagcat gatgtatcag gagaaactct taggaaggtc aacgatgaat tattttgacc
    14401 tactaaatcg tctctgattt ctttgaaccg gaggctattt gaaacggcgg ctcgcgtact
    14461 tcttttcaag tcatgtgatg gcgcgaaaag cccttagcgc agccatctga ttcgccagaa
    14521 caccgcgagc aggccgaatg cgatcacgag cataaatcca atgagtaaat agaaaccgct
    14581 gtgcaatccc aagaagggca tgttcttgaa gttcattccg aagattccgg tag
//
LOCUS       DATIOW010000176        14358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1193639, whole genome shotgun sequence.
ACCESSION   DATIOW010000176 DATIOW010000000
VERSION     DATIOW010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14358
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193639"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VLL03_05865"
     CDS             <1..562
                     /locus_tag="VLL03_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HSS46921.1"
                     /translation="NWKPLNAGVAADFLPIPNPEYGHDTHCMRLHPLAPDLLYQQNHC
                     GIYRLERPATRWERIGTNMPKKIGDIGFPLVLHPRDPATLWVFPMDGTSVWPRTSPGG
                     KPAAYVTRNAGKTWMRLDAGLPRCQGWFTVKRQAMTADAHNPVGVYFGTTSGEVWASR
                     NEGAKWSCIARHLPHIYAVEAAELGR"
     gene            559..819
                     /locus_tag="VLL03_05870"
     CDS             559..819
                     /locus_tag="VLL03_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HSS46922.1"
                     /translation="MKVLIPSPLRSYTGERNKVEAAGATLVALLADLDRRYPGIRFRM
                     IDEQDAIRRHVRIFVNGEQVRALNVPLRASDEVIILQALSGG"
     gene            1167..1373
                     /locus_tag="VLL03_05875"
     CDS             1167..1373
                     /locus_tag="VLL03_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46923.1"
                     /translation="MAYGARDLGVSLAHELAHVLMDSGEHVNEPDSLMNKESSPHSLK
                     LSPAQCERIRVSGSGNGLLQPVTR"
     gene            complement(1381..1602)
                     /locus_tag="VLL03_05880"
     CDS             complement(1381..1602)
                     /locus_tag="VLL03_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HSS46924.1"
                     /translation="MGEHLMEDLDIEALKNRINELKIQHRDLDEVIARLSESPTVDQL
                     QLQRLKKRKLLLKDQIFMLEGLLTPDIPA"
     gene            1708..2301
                     /locus_tag="VLL03_05885"
     CDS             1708..2301
                     /locus_tag="VLL03_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HSS46925.1"
                     /translation="MIPAENAFCIPDPVSHVKLAPNRNPDLSWSDVSKGAKSLVLVCH
                     DPDVPSRPDDVNREGCIVPASLRRVDFYHWVLVDLALDAAPIKKGEFSDGVTPRGKKG
                     PEALRGTRQGINDYTGWFKGDKEMSGNYFGYDGPCPPWNDAIPHHYIFTLYALDIAKC
                     PVQGNFTGADVLKAISGHILAKASLTGVYSLNPAVKL"
     gene            2386..3339
                     /locus_tag="VLL03_05890"
     CDS             2386..3339
                     /locus_tag="VLL03_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46926.1"
                     /translation="MQPRRNEFDVTNQVNTTDSVEVNDEIRRIYLGLYPDAVPEVLDQ
                     AFADLTRLYQGQYSGYLGCDTPYHNIQHVLEVSLAMARLMDGYERAQHGSEPFGERLF
                     RLGLVTALFHDCGYIRHKKDTRHKHGAEYTLRHVTRSGMFLKQYLPKLGMTDLAGVAS
                     ELVHFTGYEIPVAQIKVPSLIFRLLGNLLGSADIIAQMADRCYLEKCHERLYPEFVLG
                     GVARKRKEDGSEEVLFTSADDLVLKTPNFYQTASKRLNETLGGAYRYEQRHFGGQNLY
                     LEEVDRNIRFAKTIAEKGDMSMLKRKPPGTLCSEGDDDETK"
     gene            complement(3370..4347)
                     /locus_tag="VLL03_05895"
     CDS             complement(3370..4347)
                     /locus_tag="VLL03_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HSS46927.1"
                     /translation="MRCVEIPAYGGPEALTLANRPLPQLQAGEVLVKVAAAGVNRPDM
                     LQRQGHYPPPPGASDIPGLEIAGEIVGLDANIAQWRIGDKVCALVAGGGYAEYCAVPA
                     PQCLPIPKGLSPIAAASLPETFFTVWSNVFDRAGLKPNETFLVQGGSSGIGVTAIQMV
                     RAFGHRVFATAGSREKCAACEKLGAERAINYKTEDFVEVIKNATRGKGVDVILDMVGG
                     DYVPRELKALADDGRIVLIAWLGGSKSTLDLGEILRRRLTLTGSSLRARPVEIKAGIA
                     RSLKEKIWPLIEAGKIKPVIHATFALNEAAQAHALMESGAHIGKIVLVV"
     gene            complement(4386..7049)
                     /locus_tag="VLL03_05900"
     CDS             complement(4386..7049)
                     /locus_tag="VLL03_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46928.1"
                     /translation="MDLKTVYTKTGKGMLEMKNKSLSKDLGSVLALVDGKSNVKNVVN
                     KEGKLPETRVKELLQKLETDGYIRVFSTGPQTMFSSGADLDFTAGLVVSEIKADAYNE
                     AKARQEAQSRPRQDRAKVEDKALRETEARARAEAEAKAKAEAEARAKAEVDVKIKAEA
                     AARAKAEAEAKAKVEAEARARAEAEAKAKREAEAKARAEAEAKARWEAEAKAKAEAEA
                     RARLEAEVKTKAEAAARAKAEAEAKVKAEAEARARLEADVKARVEAAARAKAEAEAKA
                     RKEAEEKAQAEARAKEEAKAKDEIEIDFGAIFEGQPKAKIEAETGAKAGLKAREGAES
                     RAKIEAEARLRADIEASVKAEEAARQAEEEEKTNKEIRAKADEADWAKAEAEIRASIK
                     ETARKEAEVKLDFISLADSPRTDFQKERKEDDPKARKEEEKRREQEAKERAKAEEKSR
                     KEAEAQAKREAKEKAKAEAEAEKQAERAEKARARAARKPFNWKPLAIGLVVLIAAGIG
                     LVHVVPLNNYIPGVEKLAVDTLHEPVTITNLRIALLPAPQVNLEGVTIGKLKDIKIDS
                     VSVPGLSLVFGSGQLDSVDVQTLTLEQDSLSRIASWGHAQGGQALRFSRVGFKNVRLA
                     LKNIPLAPLSGELTFASDGKMLKASVRTMDNKISAQINAKRDEFVVTVSAKDWQLPLG
                     PELEFEYLDAQGTAGQDGVQLYDIDAKLYDGVAKGTAALKWDNGWKLSGDFDAKGMEL
                     KPVLNIFARNFSMNGKLDTRATYSMQAQLPEKLFEAPRVEGSFSIIRGSLNNMDLIRA
                     LQAPTRDGVRGGKTLFDEFVGKLYFSDGRYKFNEMKLVSGPLTAVGNADISPDKKLVG
                     RANVEIKSKATYLKNSFVVSGNLNDLVLYPH"
     gene            7296..7949
                     /locus_tag="VLL03_05905"
     CDS             7296..7949
                     /locus_tag="VLL03_05905"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HSS46929.1"
                     /translation="MLVLAHRGFHRSHPENTLAAFEAAVKLKLDGIETDVRLSGDGLP
                     VLIHDRVLHKDKPVAELARREAEVLLGHNVPTLDEALDAFGDILWNIEIKTPDALPAT
                     LKILRRYQKTRRLLLSSFRHDVVLQCAEALDVECALLSAQRPTQLRELLNAAENRPKI
                     RTLVWDYNILDETLLHQAAASGWHSWVYGAVTLAEHRRCAQFNLAGLITDYPQFLQK"
     gene            7953..9437
                     /gene="glpK"
                     /locus_tag="VLL03_05910"
     CDS             7953..9437
                     /gene="glpK"
                     /locus_tag="VLL03_05910"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HSS46930.1"
                     /translation="MSFILALDQGTTSSRAIVFDHAGVVRAAAQKEFAQHYPQPGWVE
                     QDPNEIWSTQIDVAKEALNRARLAASDIAAIGIANQRETTVVWDRHTGNPICNAIVWQ
                     DRRTAEYCDQLKQQGLEPTVSAKTGLLLDPYFSASKMHWILANVPNVRKQAEAGNLAF
                     GTVDSWLIWKLSNGAAHVTDASNASRTLLYNIHTGDWDDKLLAIFGVPRSLLPGVRGS
                     SGVAAETDLLGGRIAIAGIAGDQQAALFGQCCTAPGMVKNTYGTGCFMLMHTGAQPVH
                     SHNKLLTTVAWRIGNRSEYALEGSVFIAGAVVQWLRDGLGIIKSSSEVEALAASVADN
                     GGVYLVPAFAGLGSPHWDAYARGAIVGLTRGSSAVHIARAALESIAYQVADVLNAMQA
                     DSHIKLTGLRVDGGATRNNMLMQFQADVLGVPVVRPKILETTALGVAYLAGLAVDYWK
                     NQQEIVSQWQVERSFEPKMSRDEAQQLLAGWQKALGRAKGWAQP"
     gene            complement(9439..9930)
                     /locus_tag="VLL03_05915"
     CDS             complement(9439..9930)
                     /locus_tag="VLL03_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HSS46931.1"
                     /translation="MLLKGACHCGAVRFTVESRTPYPYMRCYCSICRKTAGGGGYAIN
                     IMGEAATLKVTGREHLSVYRARIEDEKGKKILSSGRRYFCRKCASALWIADPHWKRWV
                     YPFASAIDTPLPVPPEHQHILLDSKARWVEVPKRKRDRYFREYPREAIIDWHKRRGLY
                     ETK"
     gene            10030..10794
                     /locus_tag="VLL03_05920"
     CDS             10030..10794
                     /locus_tag="VLL03_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HSS46932.1"
                     /translation="MAKIAVFNQKGGVGKTTTTLNLAAALARQGREPLAIDLDPQAHL
                     SVISGVAAESGHDSIYGFYHNTRTLTALIRNPGAGWQIIPSHVELSKVGTQFGKGPNA
                     LNRLNIGIIRENLNTGRPILIDCCPMLGVLSLNAIFASDRVLVPISADYLAVKGVVQV
                     EKTLRALEQVLKKRVERRYVITRFDARRKMSWIIYNHVREKFGAELCETRISENVSVA
                     ESPAANKDVFTHAPESRGAQDYHALLCELMACGFVQ"
     gene            complement(10795..11010)
                     /locus_tag="VLL03_05925"
     CDS             complement(10795..11010)
                     /locus_tag="VLL03_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46933.1"
                     /translation="MTKFGFKIRTRRGLTVDNLIIHGRDLADAERKLDQMYLRCEILE
                     RRVIQKKEGTAAFEDIVSLIASQDEPH"
     gene            complement(11023..11421)
                     /locus_tag="VLL03_05930"
     CDS             complement(11023..11421)
                     /locus_tag="VLL03_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HSS46934.1"
                     /translation="MQKKVLTLEMAKRIAAAAEAKANANQWKVVIAVLDDGGHLVYLQ
                     RTDGTQFGSVEVAIGKAYSAIAFKRPSKGWEERLAEGRLGFLSFPKDFVLVEGGLPLT
                     LNGEIIGSIGVSGVQSSQDGEIAAAGAAAL"
     gene            complement(11436..11990)
                     /locus_tag="VLL03_05935"
     CDS             complement(11436..11990)
                     /locus_tag="VLL03_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HSS46935.1"
                     /translation="MAKNIKEEAVIFSLGERKVKFVGEDHFIADNAVVIGTVVMENNS
                     SIWFNVVARGDNDVLTVGENSNVQDGSVLHTDRGVKLTIGKNVSVGHLVMLHGCTIGD
                     NSLIGINSVILNHAVIGKNCIIGANALIPEGRVIPDNSVVLGSPGKVMRHVNADEIAM
                     IKWNADDYVKNFKHFKKQLKPDPR"
     gene            12030..13403
                     /locus_tag="VLL03_05940"
     CDS             12030..13403
                     /locus_tag="VLL03_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HSS46936.1"
                     /translation="MNLYRLLNQRAESGKPLRVGLIGAGKFGSMFLAQARRTPGVHLM
                     AIADLAPSRAHDALRRTGWPNEQFGARSFPEASKRGTTRVTDDAMALITADGIEIVID
                     ATGNPAAGIRHVLACCEHKKHIVMVNVEADALAGPLLARRAQAAGIIYSLAYGDQPAL
                     ICELVDWARAAGFEVVAAGKGTKYLPEYHASTPDTVWGHYGFSEEMVKHGDFNAQMFN
                     SFLDGTKSAIEMAAVANATGLNPAPQGLSFPPCGVDELPQLLRPRSEGGVLERSGIVE
                     VVSSLKRDGTPVARDLRWGVYVVFKASSDYAARCFKEYGLVTDDSGRYAAMYKPYHLI
                     GLELGISVASVGLRNEPTGASRDFHGDVVAVAKRDIKAGEVLDGEGGYTVYGKLMPAR
                     DSLLLSGLPIGLAHGVKLKKAIAAGTPLTWSDVTISEENPAIRFRREMEKLFKEERVT
                     GTAVSCP"
     gene            complement(13466..13741)
                     /locus_tag="VLL03_05945"
     CDS             complement(13466..13741)
                     /locus_tag="VLL03_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46937.1"
                     /translation="MRINITAFSLAAGLFWGGAIIVTGVANLIWPSYGQAFLELAASI
                     YPGYHAGANIGQVIIGALYGLVDGAVGGFVFGWLYNSISHCLPGKTA"
     gene            complement(13801..14175)
                     /locus_tag="VLL03_05950"
     CDS             complement(13801..14175)
                     /locus_tag="VLL03_05950"
                     /inference="COORDINATES: protein motif:HMM:NF039800.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HSS46938.1"
                     /translation="MREQFVGEAIKPVVSTCDTSRMAVGEPGLPREFLWRGRTIKVAA
                     ALRTWRETGKCRNGSPELYVRKHWFEVVATSGGTMKLYFERQPRRGQKGARWWLFSIL
                     ESEETDPVLDARRTRRAPEPAP"
BASE COUNT         2837 a         4184 c         4101 g         3236 t
ORIGIN      
        1 gaactggaag ccgctcaatg cgggcgtcgc cgctgatttc ctgccgattc cgaacccgga
       61 gtacggacac gatacacatt gcatgcggct gcacccgctc gcgccggact tgctctatca
      121 gcagaaccac tgcggcatct accgcttgga acgccctgcg acacgctggg agcgcatcgg
      181 cacgaacatg ccgaagaaaa tcggcgatat cggctttccc ctggtgctgc atccgcgcga
      241 tccggccacg ctgtgggtgt tccccatgga cgggacttcg gtgtggccgc gcaccagtcc
      301 cggcggcaag cccgcggctt acgtgacccg caacgcaggc aagacctgga tgcggctgga
      361 tgcaggcctg ccgcgctgcc aagggtggtt caccgtgaaa cgccaggcaa tgacggccga
      421 cgcgcacaat cccgttggcg tgtacttcgg cacgacgagc ggcgaagtgt gggcgagcag
      481 aaatgaagga gcgaagtggt cctgcatcgc tcgccacctg ccgcacatct acgcagtcga
      541 ggccgccgaa ctcgggcgat gaaggtgctg atccccagcc cgctgcgttc ctacaccggc
      601 gagcggaaca aggtggaagc cgccggcgcg acgctcgtcg cgctgctcgc cgatctcgac
      661 cggcgctacc ccggcattcg cttccgcatg atagacgagc aggacgcgat ccgccgccac
      721 gtccgcatct tcgtcaacgg cgagcaggtg cgcgcgctca atgtgccgtt gcgagcttcg
      781 gatgaggtca tcatcctgca ggccttgagc ggcggctagc gaaacgcgcg tgctacgaaa
      841 agaaaggcca ggcttcgaac gcccggcacg gtgttgcctg acgtctgcta aatcggggat
      901 accacagagt cgaattattt ccggcgtaac aatgcggctc cggccgcttt ttagttcttt
      961 ttaatttgat ggtcggtcaa cgacgggtgg attcacagtc gccgcagtgc aaagccctat
     1021 gacaggcact tgcctggccg gcggccatca ctcaaaggac cggggcagtt cgccgctgtc
     1081 agggaccacc ttcagccagc cggaggggca ctcggacgcc tctggatagt tacagcttcg
     1141 tatcggggca gtagtatcga taatgggtgg cgtacggcgc gagagatttg ggcgtaagcc
     1201 ttgcccacga actcgcgcac gtgctgatgg acagcggcga gcacgtcaac gagccggata
     1261 gtctgatgaa caaggaaagc tcgccgcata gcctgaagct cagtcctgct cagtgcgagc
     1321 gcattcgggt cagcggcagt ggaaacggtt tgctccagcc ggtcacacga tgaatgcgcg
     1381 tcaagcagga atgtcgggcg tgagcaaacc ctcgagcatg aaaatctggt ctttcagcag
     1441 caatttgcgc tttttcaggc gctgcaattg cagctgatcc accgtaggcg actccgacag
     1501 tctggcgatg acttcgtcca ggtcgcgatg ctggattttc agctcgttga tgcgattttt
     1561 tagcgcttcg atatcgaggt cttccatgag gtgttctccc atgttttgta atatagcaaa
     1621 ttcagcgctt gttataatgg cttgaactaa aaaagcaagg attatgcata cgtaaagctc
     1681 acaagttctt gtttcgcccc caatcatata atccccgcgg aaaatgcttt ttgcattccc
     1741 gaccccgtaa gccacgtgaa gcttgcgccc aaccgcaatc cggatttgtc ttggagcgat
     1801 gtgtcaaagg gggcgaaatc gctggtgctg gtttgccacg atcccgatgt gccgagcagg
     1861 cccgatgatg tgaaccgtga ggggtgcatt gtgccggcca gccttcggcg cgtggacttt
     1921 tatcattggg tgctggtgga tttggctttg gatgcggcgc ccatcaagaa aggcgagttt
     1981 tccgacgggg taacgccgcg cggcaaaaaa gggcctgaag cgctccgcgg cacgcgccag
     2041 ggcatcaacg attacaccgg ctggtttaag ggcgacaagg agatgagcgg caattatttt
     2101 ggctacgacg gaccctgtcc gccgtggaat gacgccatcc cgcaccatta catctttacc
     2161 ttgtatgcgc tggatatcgc caaatgcccg gtgcagggga acttcaccgg cgccgatgtg
     2221 ctcaaggcga tttcgggcca tattctggcc aaggcgagtc tgaccggcgt ctatagcctg
     2281 aatccggcgg tgaagcttta gccggtgtga aatacgccat tgaaaaaacc ctggcaaagc
     2341 ggtgtaatag ggcacgagtt ttatacccct gaggaggtgg gtaaaatgca gccgcgacgc
     2401 aatgaattcg acgtcaccaa ccaggtaaat accactgatt ccgttgaagt taatgatgaa
     2461 attcgccgga tttacctcgg actctatccc gatgccgttc ccgaagtcct ggaccaggcg
     2521 tttgccgact taacccggct ctaccaaggc caatactccg gatatttggg ctgcgatact
     2581 ccttatcaca acatccagca tgtgctcgaa gtatccttgg ccatggcgcg gctgatggat
     2641 ggctatgagc gtgcccagca cggtagcgag ccgtttggcg agcggctgtt caggctgggg
     2701 ttggtcaccg cgctgttcca cgactgcggc tatatccgcc acaagaaaga cacccgccac
     2761 aagcacggcg cggaatacac gctgcgccac gttactcgaa gcggcatgtt tctgaaacag
     2821 tacttaccca aactgggcat gacggatctc gcgggcgtcg cttctgagct cgtgcacttc
     2881 accggctacg aaatccccgt ggcgcaaatc aaagtgccga gcctgatttt ccgcttgctc
     2941 ggcaacctcc tgggttcggc cgacatcatt gcgcaaatgg ccgaccgctg ctatctggaa
     3001 aaatgccatg agcggctgta tccggaattc gtgctggggg gcgtggcaag gaagcgcaag
     3061 gaagacggca gcgaggaggt gttgtttact tcggctgacg acttggtgct caaaaccccc
     3121 aatttttacc agaccgctag caagcggctt aacgaaacgc tgggcggcgc ttaccgctac
     3181 gagcagcgcc attttggtgg acagaacctc tacctcgaag aagtggaccg caacatccgt
     3241 tttgccaaga ccatcgccga aaaaggcgat atgtccatgc tcaagcgcaa gccgccgggt
     3301 actttgtgca gcgagggtga cgacgacgaa accaagtgaa tcaaccgttc cgcccagcga
     3361 cgcctctctt cacacaacca gcacgatctt gccgatgtga gcgccgcttt ccatgagcgc
     3421 gtgcgcttgt gccgcctcgt taagcgcaaa cgtggcatga attaccggct tgatttttcc
     3481 ggcttcaatt agcggccaga ttttttcctt cagacttctc gcgattcccg ccttgatttc
     3541 gaccgggcgc gcacgcaagc tcgagccggt aagggtgagc cgccggcgca gaatttcgcc
     3601 caaatccagc gtggacttcg agccgcccag ccaggcaatc agtacaatgc gcccgtcgtc
     3661 tgctagcgct ttcagctcgc gcggcacgta gtcgccgccc accatgtcga gaatcacatc
     3721 cacgcctttg ccccgggtgg cgttcttgat cacttccaca aaatcttccg tcttgtaatt
     3781 gattgcgcgt tccgcgccca atttctcgca cgcggcgcat ttttcccggc tgcccgcggt
     3841 ggcgaaaaca cgatggccaa acgcgcgtac catttggatg gcagtgacgc cgatgccgct
     3901 ggagccgcct tgcaccagga aagtttcatt aggcttgagt cccgcgcggt cgaacacgtt
     3961 gctccagacg gtgaaaaaag tctccggcag cgaagccgct gcaatcggac tcaaaccctt
     4021 ggggatggga aggcattgtg gagcggggac cgcgcagtat tcggcatagc cgccgcccgc
     4081 gaccagcgcg cagactttgt cgccgattcg ccattgggcg atatttgcat cgagcccgac
     4141 gatttcgccc gcaatttcca ggccgggaat gtcagaagcg cctggcggcg gcggataatg
     4201 cccctgccgc tgcagcatgt cggggcgatt gacgcccgct gcggcgactt taaccagcac
     4261 ctcacccgcc tggagttgcg gcagtggacg gtttgcaagt gtgagcgcct caggcccgcc
     4321 ataagccggg atttccacgc aacgcatgct ggctggcaga gcgggactca ttgcttaacg
     4381 ccgagttagt gggggtacaa caccaggtcg ttaagattgc cggacacgac aaaagaattc
     4441 ttcaggtagg ttgccttgga tttgatctcg acattggcgc gcccgacgag tttcttgtca
     4501 ggcgagatgt cggcgttgcc cactgcggtt agcggccccg agaccagttt catctcgttg
     4561 aacttgtagc ggccgtcgct gaaatacagc ttgccgacga attcgtcgaa caaggttttg
     4621 ccgccgcgga cgccgtcacg ggtcggtgct tgcagcgcgc ggattaaatc catattgttg
     4681 agggagcccc tgatgatgct aaagctgcct tcaacgcgtg gcgcctcgaa cagtttttcc
     4741 ggcaattgcg cctgcatgga ataggttgcg cgcgtatcca gcttgccgtt catggagaaa
     4801 ttgcgcgcga aaatgttgag caccggcttg agttccatgc cttttgcgtc aaaatcgccg
     4861 ctcaacttcc acccgttgtc ccatttcagg gcggccgtgc ctttagccac gccgtcgtaa
     4921 agcttggcgt caatatcgta caattgcacg ccgtcttggc cggcagtacc ttgggcatcc
     4981 aggtactcga actcgagctc cgggcctagc ggcaattgcc agtccttggc gctgaccgtt
     5041 actacgaatt cgtcgcgctt ggcattgatt tgcgcgctga ttttattgtc catggtcctg
     5101 accgaagctt tcagcatctt gccgtcactg gcgaaagtga gttcgccgct tagcggcgca
     5161 agcggaatgt ttttgagcgc cagccgcacg tttttaaacc ccacccgact aaatcgcagg
     5221 gcctgaccgc cttgcgcgtg gccccacgag gcaatgcggg acaacgaatc ctgctcgagg
     5281 gtcagggtct gcacgtccac gctgtcgagt tgaccgctgc cgaacaccag gctgagtcca
     5341 ggcacgctca ccgaatcgat tttgatgtct ttgagtttgc cgatcgttac accctcgaga
     5401 ttgacctggg gagcaggtag cagcgcgatc cgcaaattgg tgatggtgac aggttcgtgc
     5461 aaggtatcga ccgcgagttt ctcgacgccg ggaatatagt tgttgagcgg aaccacgtgc
     5521 accaagccga tgcccgccgc aattaaaacc accaatccga ttgccagcgg cttccagttg
     5581 aacggcttgc gggcagcccg ggccctggcc ttttccgctc tctccgcctg tttttccgct
     5641 tcggcctcgg ctttggcttt ttccttggct tcgcgcttgg cttgagcttc agcttctttc
     5701 cgggattttt cttcggcctt ggcgcgctct ttcgcttcct gttccctgcg tttttcttct
     5761 tcctttcttg cttttgggtc gtcctctttt ctttccttct ggaagtcggt ccgcggcgag
     5821 tcggctaggg atatgaaatc gagtttgact tccgcttctt tccttgccgt ttccttgatg
     5881 ctggcgcgga tttctgcctc cgccttggcc caatcggcct cgtccgcttt ggcccggatt
     5941 tctttattgg ttttttcctc ttcttcagcc tgccgcgccg cttcctcggc ttttacactg
     6001 gcttcgatat cggcgcgcag gcgcgcttcc gcttcgattt tcgctctcga ctcggctcct
     6061 tcccttgctt tcaatcccgc tttggctcca gtctccgcct cgattttggc cttgggttgt
     6121 ccctcaaata ttgcaccgaa atcgatctcg atctcgtcct tagctttcgc ttcctctttt
     6181 gcccgcgctt ccgcttgcgc tttctcttcc gcttctttcc tggccttagc ctccgcttcc
     6241 gccttggcac gtgctgccgc ttcgactctg gctttgacgt cggcttcgag cctagcgcgt
     6301 gcttccgctt ctgcctttac tttagcctcc gcttccgcct tggcacgtgc tgccgcttcg
     6361 gctttggttt tgacttctgc ttcgagcctg gcgcgtgctt ccgcttccgc cttggcttta
     6421 gcctccgctt cccagcgcgc tttggcttct gcttctgccc gcgctttcgc ttcagcttcg
     6481 cgtttggcct tggcttccgc ttcggcacgg gcgcgggctt ccgcctccac cttggcttta
     6541 gcctccgctt ccgccttggc acgtgctgcc gcttcggctt tgattttgac gtctacttct
     6601 gctttggcgc gggcttcggc ctccgccttg gccttggctt ccgcctcggc gcgtgcgcgc
     6661 gcttcggttt ccctgagagc tttatcctcg actttggcgc ggtcctgcct cggtctgctt
     6721 tgtgcttcct gccgtgcctt ggcttcgttg taggcgtcgg ctttgatttc ggagacgacc
     6781 aggccagcgg tgaaatcgag gtcggcgccg ctggagaaca tggtttgcgg gccggtggaa
     6841 aatacgcgaa tatagccgtc ggtttcgagc ttttgcagga gttccttgac cctggtttct
     6901 ggaagcttac cctctttgtt gacgacattc ttgacgttgg acttgccgtc gaccagcgcc
     6961 aaaacactgc cgaggtcctt ggacagcgat ttgttcttca tttccagcat gcccttgccg
     7021 gttttggtgt agacggtttt caagtccatt cgcgcaaatc caccctaata aagagtgcct
     7081 ttgattggtt ccaatcgatt aagcccgctg tttgctgcag tcgcaaccgg gttaagaccg
     7141 gtgttcctcg catattatat gcagcttatt gattccctga cagtggaaaa gcgtttgccg
     7201 aaaaaaccgg gcaaagccag ccttggttcg gctaagatac aagataaaac ggaaaacttt
     7261 caagcctcaa gcatttattg cccatccaca ataccatgtt agttctggcg catcgcggct
     7321 ttcatcgttc tcatccggaa aacacgctcg ccgctttcga ggccgcggtg aagctcaagc
     7381 tcgacgggat agaaaccgac gtgcgcttaa gtggcgacgg cctgcccgtg ctgattcacg
     7441 accgcgtgct ccataaagac aagccggtgg cggagctggc gcggcgtgaa gcggaagtac
     7501 ttctcggtca caacgtgccg acattggatg aggcgctgga cgcctttggc gatattctgt
     7561 ggaacataga aatcaagaca cctgatgcgc tgccggcgac gctgaaaatc ctgcggcgct
     7621 accagaagac acgacgcctg ctgctgagtt cgtttcgcca tgatgtggtg ctgcaatgcg
     7681 ccgaggcgct cgatgtggag tgcgccttgc tcagtgcgca acgccctacg cagttgcgcg
     7741 aattgctcaa cgcagccgaa aatcggccga aaatccggac cctggtttgg gactacaata
     7801 tcctcgatga aacattgctc caccaagcgg cggcttcagg ctggcattcg tgggtttacg
     7861 gcgcggtcac tttagccgag caccggcgct gtgcccaatt taatctcgcc ggtctgatta
     7921 ccgattatcc ccagtttctg cagaaataag ccatgtcttt catcctggcg ctggaccaag
     7981 gcacgacgag ttcccgcgcc atcgttttcg atcatgcagg cgtcgtccgg gccgcagcgc
     8041 agaaggaatt tgcacagcat tatccgcaac ctggctgggt ggagcaagac ccgaacgaaa
     8101 tctggtcaac ccagatcgat gttgcgaagg aagccttgaa ccgcgccagg ctcgccgcat
     8161 ccgacattgc ggccatcggc attgccaacc agcgcgaaac tacggtggtc tgggaccgcc
     8221 ataccggcaa cccaatctgc aacgccatcg tctggcagga ccgccgcact gccgaatatt
     8281 gtgaccaact gaaacagcag gggcttgaac cgacggtctc cgccaagact gggcttttgc
     8341 tcgatccgta tttttccgcc agcaaaatgc actggattct tgcaaatgtc ccgaatgtgc
     8401 gcaaacaagc ggaggcaggc aatctggctt tcggcacggt ggacagctgg ctcatctgga
     8461 agctttcgaa cggcgcggcg catgtaaccg acgccagcaa tgcctcgcgc acgctgctct
     8521 acaacattca taccggcgat tgggatgata aactgctcgc gattttcggc gtgccgcgct
     8581 ccttgctgcc cggagttcgc ggctcgagcg gagtcgcggc cgagaccgat ctgctgggcg
     8641 gacgcatagc gattgccggc atcgccggcg accagcaggc ggcgctcttc ggccagtgct
     8701 gcaccgcccc ggggatggtg aaaaacactt acggcactgg ctgtttcatg ctcatgcaca
     8761 ccggcgccca acccgttcat tcgcacaaca agcttttgac taccgtggcc tggcgcattg
     8821 gaaatcgcag cgaatacgcg ctggaaggca gtgtgttcat cgccggcgcc gtggtgcaat
     8881 ggctgcgcga cggcctgggc atcatcaaat catcgagcga agtggaagcg ctggcggcaa
     8941 gcgttgcgga taacggcggt gtttatctgg tgccggcatt tgccggtcta ggcagcccgc
     9001 attgggatgc ttatgcccga ggcgccatcg tcggacttac ccgcggatca tccgccgtgc
     9061 acattgcgcg cgccgcgctg gagagcatcg cttaccaggt cgccgatgtg ctcaacgcca
     9121 tgcaggccga ttcgcatatc aagctcacgg gactgcgcgt ggacggcggg gcgacgcgga
     9181 acaatatgct catgcaattc caggcggatg tgctgggcgt gccggtggtg cggccgaaga
     9241 ttttagaaac taccgccctt ggtgtggcct accttgccgg cctggccgtg gattactgga
     9301 aaaatcagca ggagatcgtt tcacagtggc aagtggaacg cagcttcgag ccgaaaatga
     9361 gccgtgatga ggcgcaacaa ttgctggcgg gatggcaaaa agcgcttggc cgcgccaagg
     9421 gttgggcgca gccctgaatc actttgtttc gtacaagccg cgccgcttgt gccagtcgat
     9481 gattgcctct cttgggtatt cgcggaaata cctgtcgcgc ttgcgtttcg gcacttccac
     9541 ccagcgcgcc ttggagtcga gcaagatatg ctggtgttcg ggcggaaccg gcagcggcgt
     9601 gtcgatcgcg gaagcgaaag ggtagaccca ccgcttccag tgcgggtcgg cgatccacaa
     9661 tgcggaagcg cattttctgc agaaatacct gcgcccagaa ctaaggattt tcttgccctt
     9721 ttcatcttca atccgcgccc ggtacaccga gaggtgttcc cggccggtta ctttgagtgt
     9781 agctgcttcg cccatgatgt tgatggcgta acccccgccc ccggcggttt tgcggcagat
     9841 cgagcagtag cagcgcatat agggataagg agtgcgcgat tcaacagtaa aacgcaccgc
     9901 tccacagtga caagctcctt tgagcagcat gattttcctc cttggctgga aatgtaaaaa
     9961 acgcctttgc caaatcgcgg aatctggcgg atattgtatt ttccgggata aaatccggtt
    10021 tatccagcca tggcaaaaat tgcagtattc aaccagaaag gcggggtggg caagaccacc
    10081 actacgctta atcttgcggc ggcgctggcc aggcaggggc gcgagccgct ggcgatagac
    10141 ctcgacccgc aggcgcatct ttccgtcatc agcggcgtag cagcggaatc cggccacgac
    10201 agtatttacg gtttttacca caatacgcgc acgcttaccg cgctgattcg caatcccggt
    10261 gccggctggc aaatcattcc ttcgcacgtg gagctttcca aggtgggcac ccagtttggc
    10321 aagggaccga atgcgctgaa ccgtctcaac atcggcatca tcagggaaaa tctgaatacg
    10381 ggacggccga ttctgattga ctgctgtccc atgctgggcg tgctgtcgct caatgcgatt
    10441 tttgcttccg accgcgtgct ggtgccgatt tccgcggatt acctcgcggt gaaaggcgtg
    10501 gttcaggtgg aaaagacgct tagagctctc gaacaagtgc tcaaaaaacg tgtcgagcgg
    10561 cgctatgtga ttacgcgttt cgacgcgcgg cgcaagatgt cgtggataat ctacaaccat
    10621 gttcgggaaa aattcggcgc ggagctttgt gagacgcgca tctcggaaaa cgtaagcgtc
    10681 gccgaaagcc ccgcggccaa caaggatgtg tttactcacg caccggaaag tcgcggcgcg
    10741 caggattatc acgcgctgct gtgcgagctg atggcctgcg gctttgtgca gtaattagtg
    10801 cggttcgtcc tggctggcga tgagggacac aatgtcctcg aaagccgccg ttccttcttt
    10861 tttctgaatc acccggcgtt ccagaatttc gcagcgcagg tacatctggt cgagtttgcg
    10921 ttcggcgtcc gccaggtccc ggccgtgaat gatcaggtta tccacggtga gtcccctgcg
    10981 ggtacgaatt ttaaagccga acttggtcat ttcctaagct tactacaacg ccgcagcgcc
    11041 agccgcggca atttccccgt cctgggacga ttgtacccct gaaacgccta tagagccgat
    11101 gatttcaccg ttcagcgtca acggcagccc gccttctact aaaacaaaat ctttgggaaa
    11161 agaaaggaag cccaaacgcc cttcagcgag ccgttcttcc caccctttgg acggacgctt
    11221 gaacgctatg gctgaataag ccttgcctat cgccacctcg acgctgccga actgcgtgcc
    11281 gtcggtgcgc tgcaggtaaa ccaggtgccc gccgtcatca agaacggcaa tcaccacttt
    11341 ccattgattg gcgttggcct tggcttcggc ggcggcggca atgcgcttgg ccatttccag
    11401 tgtcagaacc tttttttgca tgggcgcctc tctaattatc gtgggtcggg tttgagctgt
    11461 tttttgaaat gcttgaaatt cttcacgtag tcgtcggcgt tccatttgat catcgcgatt
    11521 tcgtctgcgt tcacatgacg catcactttg cccggcgagc ccaggaccac tgagttgtcg
    11581 ggaatgaccc taccctctgg aatcagggcg ttcgcgccga taatgcagtt tttgccgatg
    11641 accgcatggt tgagaatcac gctgttgatg ccgatcaggc tgttgtcgcc gatggtgcag
    11701 ccgtgcagca tgacgagatg gccgaccgag acattcttgc cgatagtgag cttcacaccc
    11761 cggtcggtat gcagcaccga cccgtcctgc acgttggagt tttcgccgac agtgaggaca
    11821 tcgttgtcac cgcgcgccac cacgttgaac cagatgctgg aattgttctc catcaccacc
    11881 gtgccgatca cgacggcgtt atcggcgatg aaatggtctt cacccacgaa tttgaccttg
    11941 cgctcgccga gggaaaatat cacggcctct tccttaatgt tttttgccat tatagcgccg
    12001 ccgcccgaca aattggctaa aatggcgtca tgaatttgta ccgattgttg aatcagcgag
    12061 cggaatccgg aaagccgctg cgcgtcggat tgattggcgc gggcaaattc ggctcgatgt
    12121 tccttgctca agcacggcgc acgccgggcg tccatctcat ggccatcgcc gatctcgcgc
    12181 cgtcgcgtgc gcatgatgcc ttgcgccgca ccggctggcc gaatgagcag ttcggcgcca
    12241 ggagtttccc tgaagcatcg aagcgcggta caactcgcgt caccgatgac gcgatggcac
    12301 tgattactgc ggacggcata gaaattgtga ttgacgccac cggcaatcct gccgctggca
    12361 tccggcatgt gctcgcgtgc tgtgaacaca aaaagcacat cgtcatggtt aacgtcgaag
    12421 cggatgcctt ggccgggcct ttacttgcgc gccgcgcgca agcagcgggg attatttact
    12481 cgcttgccta cggcgatcag ccggcgctga tttgcgaact ggtggattgg gcgcgcgcgg
    12541 caggttttga agtcgtcgcc gcaggaaagg gcaccaaata tttgcctgaa taccatgctt
    12601 ccacgcctga caccgtatgg ggtcattacg gtttcagcga ggaaatggtc aagcacggtg
    12661 atttcaacgc gcaaatgttc aactcattcc tggacggcac caaatcggcg attgaaatgg
    12721 cggcggtggc gaatgctacc ggactcaatc ccgcgccgca gggattgagt tttccgccgt
    12781 gcggtgtgga cgaattgccg caattactgc gtccccgatc ggaaggcggc gtgctggagc
    12841 gctccggtat tgtggaggtg gtatcgagcc tcaaacgcga cggcacgccg gttgcgcgtg
    12901 atttgcgctg gggcgtgtat gtcgtattca aagcgtccag cgattacgca gcgcgctgct
    12961 tcaaggaata cggcttggtg actgacgaca gcgggcgcta tgcagccatg tataaacctt
    13021 accacctcat cggtctggaa ttgggaatca gtgttgcttc agtcggcttg cgcaacgaac
    13081 cgaccggcgc atccagggat tttcacggcg acgtggtggc ggtggccaag cgcgatataa
    13141 aagctggaga ggttttggat ggcgaaggtg ggtacaccgt ttacggaaag ctcatgccgg
    13201 ctcgcgattc actgttgctt tctggtctgc ccatcggcct tgcgcatgga gtgaaattga
    13261 aaaaagcaat tgccgcaggt actccgctga cctggagtga cgtgacgatc agcgaagaga
    13321 accctgcgat tcgcttcagg cgggaaatgg aaaaactgtt caaagaggaa cgggtaactg
    13381 gaaccgctgt ttcgtgtccg tagtgattag cttcccggaa aattatcgta ggctggtttc
    13441 agacgagtat taccggagcg cagattcagg ccgttttccc cgggagacag tgcgatatgg
    13501 aattatagag ccagccgaac acaaaacccc caaccgcgcc gtccaccaac ccatataacg
    13561 ctccaataat aacctgcccg atattagcac cggcatggta gccgggataa atcgaggcag
    13621 cgagttcaag aaaagcctgg ccgtaggacg gccatatcag atttgccacg ccggtaacaa
    13681 tgattgcgcc gccccaaaac aatccggctg cgagggaaaa cgcagtgata ttgattcgca
    13741 taatacctcc ttcgccaaag tgagtcggtt ggaacgatac acccagaaat ttaagcaatc
    13801 ctaaggcgct ggctcagggg cgcgccgcgt gcggcgcgcg tccagcacgg ggtcggtttc
    13861 ctccgactcg agaatgctga acaaccacca ccgggcgcct ttctgtccac gacggggctg
    13921 ccgctcgaaa tacagcttca tcgtaccgcc ggaagttgca acgacctcga accagtgctt
    13981 gcgcacatac aattcgggac tgccgttgcg gcacttcccg gtttcgcgcc aggtgcggag
    14041 cgccgccgca acttttatag ttcggccccg ccacaggaac tcgcgcggga gtcccggctc
    14101 cccaactgcc atgcgtgaag tgtcgcacgt ggagaccacg ggtttgattg cttcgccgac
    14161 aaactgctca cgcatgatta ttgttcagtt ggtccaacgt gaaatagact gcctaaaaac
    14221 agctttacat gggagtcatt ctacgcccgt tttttcctcc ctcaacggga tgggtacgca
    14281 aagaccaacc acttgggcaa tgatcagata tcagtttccg gccccttgcg atttttgcag
    14341 gaattgcaac accgcgac
//
LOCUS       DATIOW010000177         3034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1703446, whole genome shotgun sequence.
ACCESSION   DATIOW010000177 DATIOW010000000
VERSION     DATIOW010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703446"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1582
                     /gene="dxs"
                     /locus_tag="VLL03_05955"
     CDS             <1..1582
                     /gene="dxs"
                     /locus_tag="VLL03_05955"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HSS46939.1"
                     /translation="EESTYDSFGAGHSSTSISAALGMAIAAAKQGGGRKAVAIIGDGG
                     MTAGLAYEALDHAGGMKDIDLLVILNDNDMSISPNVGALSSYLARLLSGKFYTTIHES
                     GKKVLNKIPAMWDLALRTEKHVKGMVTPGPGTFFEELGFNYIGPISGHDIPTLVKILK
                     NLRDLKGPRLLHVVTRKGKGYAPAEADPVKYHGVTKFDPTVGIVPSKSKPGSPTYTQV
                     FGDWLCDMAALDERLVGITPAMREGSGLVKFSEVYPDRYFDVGIAEQHSVTVAAGMAC
                     DGLKPVVAIYSTFLQRAFDQVIHDVVIQNLPVLFAIDRAGLVGPDGPTHNGSYDFSYL
                     RCLPNMVVMAPADENECRQMLYTGFRHNGPAAVRYPRGSGPGVAVQKEMTLLPVGRAE
                     LRRQGKKIALLAFGTMVAPALEIAGKFDATVVNMRFVKPLDEEMILKLADTHDCLVTI
                     EENVIHGGAGSGVNECLVAHGMQIPVLNLGLPDRFIEHGSREEMLADAGLDKDGIARK
                     VEKFYGSRVSPDKAAVGK"
     gene            1797..>3034
                     /locus_tag="VLL03_05960"
     CDS             1797..>3034
                     /locus_tag="VLL03_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HSS46940.1"
                     /translation="MAKLRILLILLLALPIRAIAAETGSVIVLPVNGAIGPATADYFH
                     RGLEQAAEQGAQMVVLQMDTPGGLDTSMRTIIKDILASPIPIVAFVAPSGARAASAGT
                     YILYASHIAAMAPATNLGAATPVQIGGMPDARGPESSESDKKKDAEKPETTRKTQKDG
                     SSDTLTRKQVHDAAAYIRGLAQMRGRNVEWAEKAVREAVSLSADEALKLGVIDLVADD
                     VTHLLKQIHGRKLNILGQERTLNTKNAKTVTFEPDWRSRLLAVIANPSIAYILMLIGV
                     YGLFFEFSNPGYVLPGVAGLICLILALYAFQLLPVNYAGLVLIMLGIAFMVAEAYFPA
                     YGSLGVGGVIAFVIGSVILIDTGVSGYGIPLPLILGIGMASALFLIFVMGMVVKARNR
                     PVVSGREELIGSIGEVTEDF"
BASE COUNT          635 a          870 c          882 g          647 t
ORIGIN      
        1 cgaagaaagt acatatgatt catttggcgc cgggcattcc agtacttcga tcagcgccgc
       61 actcggcatg gccattgccg ccgccaagca aggcggcggc cgcaaggcgg tggcgattat
      121 cggcgacggc ggcatgaccg cgggtcttgc ctacgaagcg ctcgaccatg caggcggcat
      181 gaaggacatc gacctgttgg tgatcctcaa cgacaacgac atgtcaattt cgcccaatgt
      241 cggcgcgctg tcgagttatc tcgcgcgact gctttccggc aagttctaca ccaccattca
      301 tgaaagcggc aaaaaggttc tgaacaagat tccggcgatg tgggacctgg cgctgcgcac
      361 cgaaaagcac gtgaagggca tggtcactcc cggccccggc acgtttttcg aggaactggg
      421 tttcaactac atcggcccca tcagcggaca cgacatcccc acgttggtca aaatcctcaa
      481 aaacctgcgc gacctcaagg ggccgcggct tttgcacgta gtaaccagga agggcaaggg
      541 ctacgccccc gccgaggccg atccggtcaa atatcacggt gtcactaagt tcgatcccac
      601 ggtgggtatt gttccttcga aatcgaagcc gggcagtccc acttatacgc aagtattcgg
      661 cgactggctg tgtgacatgg cggcgctgga tgaacgactg gttggcatca cgcccgccat
      721 gcgtgaaggc tcgggtctgg tcaaattttc cgaagtttac cccgaccgct atttcgatgt
      781 cggcatagcc gagcagcaca gcgtcacggt tgccgcaggc atggcctgtg acggcttaaa
      841 accggtggtc gcgatttatt ccactttcct gcagcgtgcc ttcgaccaag tcattcacga
      901 cgtcgtgatt cagaatttgc cggtgttgtt cgcgattgac cgcgccggcc tggtcggccc
      961 ggacgggccg acgcacaacg gcagctacga tttctcctac ctgcgctgct tgcccaacat
     1021 ggtcgtcatg gcgccggcgg atgaaaacga atgccggcaa atgctctaca ccggcttccg
     1081 tcacaacggc ccggcggcgg tgcgctatcc gcgtggcagc ggcccgggcg tggcggtgca
     1141 gaaggaaatg acgctgctgc ccgtaggccg cgcggagctg cgccgtcaag gcaagaaaat
     1201 tgctttgctg gcttttggca cgatggtcgc accggctttg gaaatcgcgg gaaaattcga
     1261 cgccaccgtc gtcaacatgc gcttcgtcaa acctcttgat gaagaaatga tcctcaagct
     1321 cgccgatacc catgattgcc tcgtgaccat cgaggaaaac gtaattcacg gtggcgcggg
     1381 cagtggtgtc aatgaatgcc tggtggcgca tggcatgcaa atcccggttc tcaatctcgg
     1441 acttcccgac cgcttcatcg aacatggctc gcgcgaggaa atgctggcag atgcgggatt
     1501 ggacaaggac gggattgcgc gcaaagtgga aaaattctac ggtagccgtg tgtccccgga
     1561 taaagcggca gtgggcaaat aatctgacgg cctagtggga attcctgctt tttgcctttg
     1621 gctgcgccat aagcttctaa gcagtacccc gtaactcaac cgtgccgaga ttatcttcgg
     1681 cattccagac taccaattta gcccgctcat gagcgggcgt tgcccagcac tccttgtttt
     1741 ccccggctgt ggccaaacaa gctatagtta aaaggcaggg atcgcacgcc aatccgatgg
     1801 ccaaattgcg catcttactc attctgctgt tggctttacc tatccgggca attgccgcgg
     1861 agacgggatc tgtcatcgtc cttccagtaa acggtgcaat cggccccgcc accgccgatt
     1921 actttcaccg tggtctggaa caagctgccg agcagggcgc gcaaatggtg gtgctgcaaa
     1981 tggacacacc gggggggttg gacacctcca tgcgcaccat tatcaaggac attctcgctt
     2041 cacccatacc cattgtggct ttcgtagcgc ccagcggcgc gcgtgccgcc agcgcaggca
     2101 cctatattct ttatgcgagc cacattgccg cgatggcgcc ggccaccaac ctgggggcgg
     2161 cgactccggt gcaaatcggc gggatgccgg atgcccgcgg accggaatcc tccgagtctg
     2221 ataagaaaaa agacgccgaa aaaccggaaa ctacccgcaa gacccaaaaa gacggcagct
     2281 cggatacatt gacccgcaag caggttcatg atgccgcagc ttatattcgc ggattggcgc
     2341 aaatgcgagg acgcaatgtg gaatgggcgg aaaaggcggt gcgtgaagcg gtcagcctgt
     2401 ccgctgatga agcgttgaag ctcggcgtca ttgacttggt ggccgatgat gtcacgcacc
     2461 tgctgaagca aatccacggc cgcaagctca acatcctggg gcaggaacgc accctcaaca
     2521 ccaaaaacgc caaaacggtc accttcgagc cggactggcg cagccgcctg cttgctgtaa
     2581 ttgctaaccc gagtatcgcc tatatcttga tgttgatcgg tgtctatggc ctgtttttcg
     2641 aattttccaa cccgggttat gtattgcccg gtgttgcggg gttgatctgc ctgattctcg
     2701 cgctgtacgc cttccagttg ctgccggtca attatgccgg actggtgttg atcatgcttg
     2761 gcattgcctt catggtcgcg gaagcctatt ttccggctta tggttcgctc ggcgtgggcg
     2821 gtgtgattgc tttcgtcatc gggtcggtga tacttatcga caccggggtg tcgggatacg
     2881 ggattccctt gccattaata ctcggaatcg gcatggcgag cgccctgttc ctgatttttg
     2941 tcatgggcat ggtcgttaaa gcccgcaatc gccccgtggt cagcggccgc gaggagctta
     3001 taggcagcat cggggaagtg acggaggatt tcga
//
LOCUS       DATIOW010000178         6912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_180314, whole genome shotgun sequence.
ACCESSION   DATIOW010000178 DATIOW010000000
VERSION     DATIOW010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180314"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VLL03_05965"
     CDS             <1..632
                     /locus_tag="VLL03_05965"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: protein motif:HMM:NF003742.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HSS46941.1"
                     /translation="PLVFSTLMNPEIGAIVASADALFLDFFQAFTGPLENELDVKSSH
                     AIGRSHSLKNSYDYQSRIEAVNYALVHDDGVSTRDLNQADIILIGVSRSGKTPTCLYM
                     ALQFGIRAANYPLLPEDFGSMTLPAQLKPFSHKLYGLTIGSERLQQIRNERKPGSRYA
                     TLANCQFEVKEAESLMRQEGIRYVNTTTKSIEEIATTILHQANLERHIY"
     gene            complement(592..1197)
                     /locus_tag="VLL03_05970"
     CDS             complement(592..1197)
                     /locus_tag="VLL03_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HSS46942.1"
                     /translation="MPELKPVVLADSLFKQISPVTTPTGILAIVEVSERAVKPQDTRF
                     CVLLEEMQDAGNLGSILRSAAAAGADAAFLSKNCAFAWSPKTLRAAMGAHFQLHLAEN
                     ADLVAVAKKFRGKLVAAEPRAKKSLYEVDLRGPVAFIIGNEGTGLSEPLLNLAHYRVN
                     ISMPGKIESLNAAAAATVCLFEKVRQEQANRYAARDWPDAK"
     gene            1198..1362
                     /locus_tag="VLL03_05975"
     CDS             1198..1362
                     /locus_tag="VLL03_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46943.1"
                     /translation="MPQQALGFLMLKAGGAQDQGLRRAKGRTISGHQMHAVEKRLARF
                     PSFPRRVRQF"
     gene            complement(1415..2719)
                     /gene="senA"
                     /locus_tag="VLL03_05980"
     CDS             complement(1415..2719)
                     /gene="senA"
                     /locus_tag="VLL03_05980"
                     /inference="COORDINATES: protein motif:HMM:NF041186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1903257 - selenoneine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine synthase SenA"
                     /protein_id="HSS46944.1"
                     /translation="MNAPFSPRPADLGKHLQDARKRTLALLGDFKGSQWLGPRLAIVN
                     PPLWELGHLGWFQERWCLRYNGSSSLLPSILPHADELYDSAAVAHDTRWDLPLPSLDR
                     TLAYLDRVLQRVLEKLQRGEKHLDYFFRLALFHEDMHGEAFYYTRQTLAYSRPPFALT
                     PKPENPSGSLNQDAAIPGGKMMLGATTDDGFVFDNEKWAHKVEIKPFKISRSAVSNRE
                     FAGFVEDGGYQRRELWSEQGWRWRREAQAVAPVYWVKESGQWMLRHFDEFTPLPPDAA
                     VIHVNWFEAEAYCRWAGRRLPTEPEWEMAAATESGNTEIKRRYPWGNAPPVKEHANLD
                     GFVCGPCSVSSFSPGDSGWGCRQMFGNVWEWTQDWFLPYPGFEIDPYQEYSQPWFGDH
                     KVLRGGCFATRARLLRNTWRNFYTPDRRDVFAGFRTCAFESG"
     gene            2773..3720
                     /gene="senB"
                     /locus_tag="VLL03_05985"
     CDS             2773..3720
                     /gene="senB"
                     /locus_tag="VLL03_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine biosynthesis selenosugar synthase
                     SenB"
                     /protein_id="HSS46945.1"
                     /translation="MNIAIITPATAPILGGNRNTAQRWARLLRVLGHRARVQACWDGR
                     RADAMIALHARKSCESIKKFSVLQPDQSLILCLTGTDLYRDIKTDASARESLQLASRI
                     IVLQEMGLQELSPELRRKTRVIYQSAKPVKPTLPLKNCFEICIIGHLREEKDPFRCVM
                     ALQHLPPSSRIRVSHMGRAMTGAMEKEARRWMAREPRYQWLGEIPHRQARRRLARSRA
                     MVISSRMEGGANVISEALMARVPIIASSIRGNIGMLGADYSGYYPLGDERRLALLLQR
                     VENDPAFRRLLNRQCKKRLPLIQEKTEKNSLKRLLAELD"
     gene            complement(3717..4115)
                     /locus_tag="VLL03_05990"
     CDS             complement(3717..4115)
                     /locus_tag="VLL03_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HSS46946.1"
                     /translation="MFFAYMALRPAAGEALELPQRLRLWNGTLRRFFNWVWFAVALLL
                     ASGFYLTHQLGGFNAPPYVYFMMAIGIVMMLIFAHVFFAPFKRLKRGVASQEWKAAGA
                     ALNQIRILVAVNLFLGLIVIGTATLGKAGF"
     gene            complement(4174..4746)
                     /gene="rnhB"
                     /locus_tag="VLL03_05995"
     CDS             complement(4174..4746)
                     /gene="rnhB"
                     /locus_tag="VLL03_05995"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HSS46947.1"
                     /translation="MSQQLICGVDEAGRGPLAGPVFAACVMLDPRRPITGLADSKQLT
                     AIKRQALAEQIKSQARAWAVAYATVEEIDELNILQASLLAMKRAVEMLSVEPAEVLVD
                     GLYCPQVKFPARAIVKGDAKVPEIMAASILAKTARDAEMRLLHQRFPQYGFDKHKGYP
                     TAQHLQALKLHGVTVIHRRSYAPVKALFRL"
     gene            complement(4715..5914)
                     /gene="lpxB"
                     /locus_tag="VLL03_06000"
     CDS             complement(4715..5914)
                     /gene="lpxB"
                     /locus_tag="VLL03_06000"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HSS46948.1"
                     /translation="MQPAPVLPTLAVVATEASGDQLGAHLIGVLKSFLPQAQFIGIGG
                     PKMQAAGLQSLFPLEKLAVHGYIEVLRRLPEILWIRSKLRSHLLKIRPHLFIGIDSPD
                     FNLGLEQALKQRGIPTIHYVSPSIWAWRGERIHKIRRAVSHILALFPFEPPLYEKAGV
                     PVTYIGHPLAALLPQTPDRAAAREQMRLAPSIKVVALLPGSRQNELKYMADTFIATAR
                     LIMQALPHSHFLVPLLSRETREQFENTLYRNNAQDLPLTILFGHAHNALMVADAALVA
                     SGTATLEALLLKCPMVITYKANDLSFRHVKGKYYLPYAGLPNILAGEFVVPEILQGDA
                     TPENLSQAVLNLLSDQTVTERLRNKFHAIHAELKQNTAERIAAAVLPYLESQGVKVES
                     AADLRSG"
     gene            complement(5919..6710)
                     /gene="lpxA"
                     /locus_tag="VLL03_06005"
     CDS             complement(5919..6710)
                     /gene="lpxA"
                     /locus_tag="VLL03_06005"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HSS46949.1"
                     /translation="MRSALDVMIHPTAIIHPGAKLAMDVKVGAYTLIGDEVEIGPGNW
                     IGPHAVIQGPTRIGANNRIFQFVSLGEMPQDKKYGGEDTRLEIGNGNTIREFCTFNRG
                     TVQDVGVTRLGDDNWIMAYVHLAHDCQIGSHTIFANNVHLAGHIHVGDYAVLGGFVGI
                     HQFCHIGAHSMLGAGSIVRQDVPPFVTAVGNTAKPRGINSEGLKRRGFTDEVITRIKH
                     AYRTLYKSGLSLEDATQSIAAEMAECPELRPLVEFLEKSQRGVIR"
     gene            complement(6689..>6912)
                     /locus_tag="VLL03_06010"
     CDS             complement(6689..>6912)
                     /locus_tag="VLL03_06010"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabZ"
                     /protein_id="HSS46950.1"
                     /translation="FKSEGFKVDRKSVYYFVGIDSARFKKPVSVGDQLLLHATLLRHI
                     KGFWKFTAHAKVGDETVAEAELICAVRSM"
BASE COUNT         1524 a         2001 c         1973 g         1414 t
ORIGIN      
        1 gtcctctggt gttcagcacc ttgatgaatc cggagattgg cgccatcgtg gcttccgcgg
       61 acgccctgtt tctggatttt ttccaggctt ttaccgggcc gctggaaaac gaactggacg
      121 tgaaatcatc gcacgccatc ggccgctcgc atagtttgaa aaacagctac gattaccaat
      181 cgcgaatcga ggcggtgaac tatgccctgg tacatgatga cggggtgtcc acgcgcgatt
      241 tgaatcaagc cgacattatt ctcatcggcg tctcgcgcag cggcaagacg cccacctgtt
      301 tatacatggc gttgcagttc gggattcgtg ccgccaacta tcccctgctc cccgaggatt
      361 tcggctcgat gacactgccg gcgcagctga aaccgttcag ccacaagctc tacggcctca
      421 ccatcgggtc cgagcgcttg cagcagatcc gcaacgaacg caaaccgggc agccgctacg
      481 ccacgctggc caattgccag ttcgaggtca aggaagcgga gtcgttgatg cggcaggaag
      541 gcatccgcta cgtcaatacc accaccaagt ccatcgaaga aatcgcgacc actattttgc
      601 atcaggccaa tctcgagcgg catatctatt agcttgctct tgccggactt tttcaaacag
      661 gcaaaccgtt gcagcggcag ccgcattaag cgattcgatt ttccccggca tggaaatgtt
      721 tacccgatag tgcgccagat tcaacaaggg ctccgataga cccgttccct cgttgccgat
      781 aataaaggca accggcccgc gcaaatcgac ctcgtataaa gacttctttg cacgaggctc
      841 ggctgcaacc agcttgcctc taaatttttt cgcgactgca actaggtcgg cattctcggc
      901 gaggtgcagc tgaaaatgtg cgcccatcgc cgcacgcaag gttttgggcg accaggcgaa
      961 ggcgcaattt tttgagagaa aggcagcgtc cgcgcctgcc gcggcggcgg aacgcaaaat
     1021 agagccgaga ttgccggcat cctgcatctc ctccagcaac acgcaaaaac gggtgtcctg
     1081 cggtttaaca gcgcgctcag aaacttcgac gatggctaag attcccgttg gtgtggtgac
     1141 gggagaaatc tgcttgaaca aagagtcggc cagcaccacc ggcttgagtt caggcatttg
     1201 ccgcagcagg ccctgggttt cctcatgctc aaggccggtg gcgctcaaga tcagggtttg
     1261 cggcgcgcca aaggacgcac aataagcggc caccagatgc atgccgtcga gaagcgcctt
     1321 gcgcgttttc cttctttccc gagacgagtg cgccagtttt aacagcgatt tatacagcgt
     1381 gttttctcgc gaagtgatgt gcttcactgc gaggttaacc ggactcgaac gcgcaggtgc
     1441 gaaacccggc gaatacatcg cgtcggtccg gcgtgtagaa gttgcgccaa gtgttgcgca
     1501 aaagcctcgc gcgcgtggcg aaacaaccgc cgcgcagaac cttgtggtcg ccgaaccacg
     1561 gttgcgaata ttcctggtag gggtcgattt caaaccccgg ataagggagg aaccaatcct
     1621 gagtccattc ccaaacgttg ccaaacatct gccggcagcc ccagccgctg tcgcctggag
     1681 aaaaactgct taccgagcaa ggaccgcaaa caaaaccatc aagattggca tgttctttga
     1741 cgggcggtgc attgccccag ggatagcgtc gcttgatttc cgtattgccg gattcggtgg
     1801 ccgccgccat ctcccattcg ggctccgtgg gcaggcgccg cccggcccag cggcagtaag
     1861 cttcggcttc aaaccaattg acatgaatga ctgcagcgtc gggcggcaaa ggcgtgaatt
     1921 cgtcaaaatg gcgtagcatc cactgcccgc tttcttttac ccaatacacc ggcgccacgg
     1981 cctgcgcctc ccgccgccag cgccagccct gctccgacca taattcgcgg cgttgataac
     2041 ctccatcttc gacaaagccg gcaaattcgc ggttgctgac tgcgctgcgg gaaatcttaa
     2101 atggcttgat ttcgacctta tgcgcccatt tttcattatc gaaaacaaag ccgtcatcgg
     2161 ttgttgcgcc cagcatcatc tttccccccg gaatagcggc gtcttgattt aaactgccgg
     2221 aaggattctc cggtttgggt gtgagagcga atggcggccg ggaataagca agagtctggc
     2281 gcgtgtaata aaacgcttcg ccgtgcatgt cctcatgaaa caatgcgagc ctgaaaaaat
     2341 aatcgagatg tttttcgccg cgctgcaatt tctccagcac gcgctgcaaa acccggtcga
     2401 gataagccag tgtccggtcg aggctgggca agggcaaatc ccagcgggtg tcgtgagcca
     2461 cagcggccga atcgtaaagc tcgtccgcat ggggcagaat cgatggcaac aaagaggagg
     2521 agccgttata gcgcagacac cagcgctcct ggaaccagcc gagatggccc agttcccaca
     2581 acggcgggtt gacgatggcc agcctggggc ccagccattg gctgccctta aaatcgccta
     2641 atagagccag ggtgcgtttc cgtgcatcct gcagatgttt tccgaggtct gccggtcgcg
     2701 gtgaaaaagg cgcgttcatt gagtgttatg ccttctgata tagtgccctc attatcgcaa
     2761 ctttagccgc tcatgaacat cgccatcatc acccccgcaa cggcgcccat actcggaggg
     2821 aatcgcaata ccgcgcaacg ctgggcgcgt ttgttgagag tgctgggcca tcgtgcaaga
     2881 gttcaagcat gctgggatgg caggcgcgcc gatgccatga tcgcgctgca tgcgcgcaag
     2941 agctgcgagt cgataaaaaa attttccgtt ttgcagccgg accagtcgct cattctctgc
     3001 ctgaccggga ccgatcttta ccgcgatatt aaaaccgatg cttcagcccg ggaatcgctg
     3061 cagctggcca gccgcatcat tgtgctgcag gaaatgggat tgcaggagct gtcgccggaa
     3121 ttgcgccgga aaacgcgggt gatttaccag tccgccaagc cagtcaaacc cacgctgccg
     3181 cttaaaaact gttttgagat atgcatcatc ggccatttgc gggaggaaaa agaccctttc
     3241 cgttgcgtca tggctctgca acacctgccg ccctcttccc gcatccgtgt cagccatatg
     3301 gggcgggcca tgaccggcgc aatggaaaaa gaggcgcggc gatggatggc gcgcgaaccg
     3361 cgctaccagt ggctgggcga aattcctcat cggcaggcgc gccggcgcct tgcgcgcagc
     3421 cgggccatgg tcataagttc aagaatggaa ggcggcgcca acgtgatttc cgaagcgctg
     3481 atggcgcggg tgccgataat cgcttcatcg attcgcggaa acatcggcat gctaggggcc
     3541 gattattccg gctattatcc gctgggagac gaacgccggc tggcgctgct gctgcagcgg
     3601 gtggaaaacg atcctgcctt tcgcaggctg cttaaccggc aatgtaaaaa gcgcctgccc
     3661 ttgattcagg aaaaaaccga gaagaacagc ctgaaacggc ttctcgccga attggattaa
     3721 aagcccgcct ttcccaaggt cgccgtgccg atgacgatga gacccagaaa caaattcacg
     3781 gcaaccagaa tgcgtatttg attgagcgcg gcgcccgcgg ctttccactc ctgtgacgcc
     3841 acaccgcgct taagacgctt gaatggcgcg aaaaacacat gggcaaagat cagcatcatg
     3901 acgataccga tggccatcat gaaataaaca taaggcggcg cgttaaagcc gcccaattgg
     3961 tgggtcaggt aaaacccgct tgccaataac agcgcgacgg cgaaccaaac ccagttgaaa
     4021 aagcggcgta atgtgccgtt ccaaagcctg agccgctgcg gaagctcaag ggcctcgccc
     4081 gctgcggggc gcaaggccat atacgcgaaa aacattccgc cgacccagat cactgctccc
     4141 aggacatgaa aaaactttaa tagagtcgac atatcataga cggaacaagg ccttgaccgg
     4201 cgcgtagctg cggcgatgga taaccgtcac gccgtgcaat ttaagcgctt gcaagtgctg
     4261 cgcggtcgga tagcctttgt gcttgtcgaa accgtattgc ggaaaacgct ggtgcagcag
     4321 ccgcatttcc gcatcccgtg cggttttggc caggatggat gccgccatga tttccggaac
     4381 tttggcgtcg cccttgacta tagcgcgggc cggaaatttc acttgcgggc agtacaagcc
     4441 atccaccagg acttcggcag gctcgacgga aagcatttcc accgcgcgtt tcatcgccag
     4501 caggctcgct tgcaaaatat taagctcatc gatttcttcc acggtggcgt aagcgacagc
     4561 ccaagcgcgc gcctgagatt tgatttgctc cgccagcgct tggcgtttga ttgcagtgag
     4621 ttgcttggaa tcggcgagcc cggttatcgg tcttcgcgga tccagcatta cgcaagccgc
     4681 gaatacgggg ccggccaagg gcccgcgccc cgcttcatcc actccgcaaa tcagctgctg
     4741 actcaacttt tactccctgg ctctcaagat acggcaggac cgccgccgcg atgcgttccg
     4801 cggtattttg cttcaactcg gcgtgaatgg catgaaattt gttgcgcaag cgctcggtca
     4861 ccgtctggtc ggagagcaaa ttaagcacgg cctgagacag gttttcggga gtcgcatcgc
     4921 cctgcaggat ttctggtacc acgaattcac cggccaggat gttggggagc cccgcataag
     4981 gcaagtaata cttgcccttg acatggcgaa agctcagatc attggccttg taggtaatca
     5041 ccatcgggca cttgagcagc agcgcttcca gcgtggccgt gccggaggcc accagcgcgg
     5101 catcggcaac catgagcgca ttgtgcgcat ggccaaacaa aatggtgagc ggcaaatcct
     5161 gcgcattatt gcggtacaag gtgttttcga attgctcgcg cgtctcccgg ctcaacagcg
     5221 gtactaaaaa atgcgaatgg ggaagcgctt gcatgatcag tcgcgcggta gcgatgaagg
     5281 tatcggccat gtacttaagc tcgttctgcc ggctgcccgg cagcagcgca acgaccttga
     5341 tgcttggggc aagacgcatc tgctctcttg ccgctgcgcg atcgggggtc tgcggcagca
     5401 atgcagccag ggggtgtcca atgtaggtga cgggcacgcc cgccttttca taaagcggcg
     5461 gctcgaaagg gaacagagcc agtatgtgcg ataccgcccg tctgatcttg tggatgcgtt
     5521 cgccgcgcca ggcccaaatt gaggggctga cataatgaat ggtgggaatg ccgcgttgtt
     5581 taagcgcctg ctccagcccc agattgaaat ccggcgaatc gatgccgatg aacaaatgcg
     5641 gacgaatttt cagcagatgg ctgcgcagct tgctgcgtat ccagagaatt tccggtagcc
     5701 gccgcagtac ctcgatgtag ccgtggacgg cgagcttttc cagcgggaac agcgattgca
     5761 ggccagcggc ctgcattttc ggcccgccga tacctatgaa ttgcgcctgc ggcagaaaag
     5821 atttcaacac cccgatcaaa tgggcgccaa gctggtcgcc cgaagcttcg gtggcgacaa
     5881 cggcgagagt cggtaaaacg ggcgctggtt gcataacgct agcggatgac cccgcgctgg
     5941 cttttctcaa gaaactcgac caagggacgc agctctgggc attccgccat ttcagcggca
     6001 atcgattgcg tcgcgtcttc caggctcaag ccagatttgt aaagcgtcct ataggcatgt
     6061 ttaatgcgag taatgacttc gtcggtaaaa ccgcggcgct tcagcccttc tgaattaatg
     6121 ccgcgaggct ttgccgtatt gcccactgcc gtcacaaagg gcgggacatc ctgccttacg
     6181 atgctgcccg cgccgagcat gctgtgcgcg ccgatgtggc aaaactggtg gatgccgaca
     6241 aacccgccca gcaccgcata gtcgccgacg tggatatggc cggcaagatg cacgttgttg
     6301 gcgaaaatgg tgtgactgcc gatctggcag tcgtgggcaa gatgcacgta agccatgatc
     6361 cagttgtcgt ctcccaggcg cgtcacgccc acgtcctgca ccgtgccgcg gttgaaggtg
     6421 cagaactcgc ggatggtgtt gccgttgccg atttccagcc gggtgtcttc tccgccgtat
     6481 ttcttgtcct gaggcatctc gcccagagag acgaactgga aaatgcggtt attggcgccg
     6541 atgcgggtcg gcccctgaat caccgcatgc gggccaatcc agttgcccgg gccgatttcg
     6601 acctcgtcac cgataagggt gtaggccccg accttgacat ccatagccag cttggcgcca
     6661 gggtgaataa tagcagtcgg gtggatcatt acatcgagcg cactgcgcat atcagctcgg
     6721 cttcggcgac ggtctcgtcc ccgaccttgg catgggcggt aaacttccag aaccccttga
     6781 tgtgacgcag cagcgtggcg tgcagcagca gctgatcgcc caccgacacc ggtttcttga
     6841 agcgggcgct gtcaatgccg acgaagtaat acaccgactt gcggtcaacc ttgaatccct
     6901 cgcttttaaa ag
//
LOCUS       DATIOW010000179         5012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1400825, whole genome shotgun sequence.
ACCESSION   DATIOW010000179 DATIOW010000000
VERSION     DATIOW010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400825"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VLL03_06015"
     CDS             <1..483
                     /locus_tag="VLL03_06015"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HSS46951.1"
                     /translation="YGALQGLNKAETAAKFGEKQVLVWRRSYDVPPPALDKSDSRYPG
                     HDAKYKNLAEKELPLTECLRDTVDRFLTYWNETIAPAVKAGRKVIIAAHGNSLRALVK
                     YLDNVSDKEIVELNIPTGIPLVYELDDRLRPLKSYYLGDQEKIKQASQAVADQGKARA
                     "
     gene            480..1670
                     /locus_tag="VLL03_06020"
     CDS             480..1670
                     /locus_tag="VLL03_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HSS46952.1"
                     /translation="MKRCWIYASCFLLVFAPAFPLYAAPTKEELKKEELKQLRGRIEA
                     LQQELASAEDEKSEAADALKESERAISEANRKLYELNRQQREINSSLATLQTQSRQTE
                     SNLRNQQALLEKLLYQAYIGQQPDYLALLLNGQDPDRLARQLHYYSYISRARADLIAR
                     LRGNLNQLATLSAQTREKSDELKAIQREHAAQKRQLEKEKAEHQRVLARLSQQITKQR
                     HEIGTLQRNEKRLTRLIERLAKILSEKKASSRLSNSRLPGPSLDASPFQQLKGKLNLP
                     VKGELANRFGSPRSDSGLTWKGLFILSPGGQEVKAVAAGRVVYADWLRGFGNLIILDH
                     GAGYMSLYGNNETLYKQVGDEIHGGDTIAAVGNSGGNPDSGLYFEMRYQGKPFDPLAW
                     VTVK"
     gene            1709..3103
                     /locus_tag="VLL03_06025"
     CDS             1709..3103
                     /locus_tag="VLL03_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HSS46953.1"
                     /translation="MRDKLQQLGLVMLGALLGVALSLNFSAVAQKEAAAPLPVEELRA
                     FTEVFGRIKNDYVEPVDDKKLITEAINGMLTGLDPHSAYLDADAFKELQVGTQGEFGG
                     LGIEVGMEDGFVKVVSPIEDTPAYNAGLRSGDLIIKLDDTAVKGMTLNDAVKRMRGKP
                     NTQITLTVVRKGESKPLVFTLTRAVIKVQSVKSKMFEPGYGYIRITQFQEHTGETLVK
                     AINDLYKPGTLKGLVLDLRNDPGGLLNSAVAVSAAFLQPNALVVYTEGRTDDAKMRLS
                     AIPENYLRGNFKEDYLKKLPAAVKSVPLVVLVNNGSASASEIVAGALQDHKRAIIMGT
                     QSFGKGSVQTILPLGNNTAIKLTTARYYTPNGRSIQAKGIVPDIMVEEATVNGVEPGP
                     RLREADLERHLSNDTQPEENPAPPKATPPKLNPKPKGEEGKVELPEFGGKNDYQLNQA
                     VNLLKGLQILQGQK"
     gene            3267..4034
                     /locus_tag="VLL03_06030"
     CDS             3267..4034
                     /locus_tag="VLL03_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HSS46954.1"
                     /translation="MNDNQLIRYSRHILLPQIGIEGQEKLLHSHALIIGAGGLGSPAA
                     LYLAAGGVGKITICDGEKVDLTNLQRQIVHHTLSVGTPKTQSARNTLGLINPEVTVVE
                     VPKRVTREDILDLVRSADVVLDASDNFATRHAVNSACVAHRKPLVSGAGVRFDGQVAV
                     FDLRETSSPCYHCLFPEEGEVEETRCAVMGVFAPLTGIIGTIQAAEALKLLTDTGETL
                     NGRLLLLDALSMEWRSIKLAKDPQCSVCGRKANAVNA"
     gene            complement(4219..4797)
                     /gene="slmA"
                     /locus_tag="VLL03_06035"
     CDS             complement(4219..4797)
                     /gene="slmA"
                     /locus_tag="VLL03_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HSS46955.1"
                     /translation="MAAKTGERKMQILQTLAQMLQNPRAEKITTAALAAKLDISEAAL
                     YRHFASKAQMFEGLIEFVEESLFGLINKITSEEQSGLKQLEAMLSMLLGFAEKNPGMT
                     RVLIGDALVNENERLQTRINQLHDRLEASLRQALRFAASRSEIPADLDTAAQANLLIC
                     FVTGRWHQYAKSGFKREPMEHWAGQWRELMRG"
     gene            complement(4787..>5012)
                     /locus_tag="VLL03_06040"
     CDS             complement(4787..>5012)
                     /locus_tag="VLL03_06040"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HSS46956.1"
                     /translation="GFLRLCSRTGARPQRCVMIDDTLENLKTAKKLGMKTIWVSTSLK
                     APSYVNMNIQSVMELLRLLGNFNLRQSNGR"
BASE COUNT         1208 a         1470 c         1383 g          951 t
ORIGIN      
        1 tacggcgcct tgcagggctt gaacaaagcc gaaaccgcgg ccaaatttgg cgagaagcag
       61 gttctggtat ggcgcaggag ctacgatgta cctcctcccg ccctcgacaa gtccgactcc
      121 cgctatccgg gacacgacgc aaaatataaa aatcttgccg aaaaagagct gcctcttacc
      181 gagtgtctga gagatacggt agatcgcttt ctgacctact ggaacgaaac aatcgctccg
      241 gcggtgaaag cggggagaaa agtcatcatc gccgcgcacg gcaattcgct gcgcgcgctg
      301 gtcaagtatc tcgataatgt ttccgacaag gaaatcgtcg aactcaacat cccgaccgga
      361 attcctctgg tctatgaact cgacgaccgc ctgcggccgc tcaaaagtta ctatttgggg
      421 gatcaggaaa aaataaaaca ggcgagccag gcggtcgccg accagggcaa agcccgggcg
      481 tgaaacgttg ctggatttac gcaagctgct tcctgctcgt gtttgcgccg gctttccctc
      541 tctacgccgc ccccaccaag gaagagctca aaaaggaaga gctcaaacaa ctgcgcgggc
      601 gcatcgaagc actgcaacag gagctggcga gcgcggaaga cgaaaaatcc gaggcggccg
      661 atgcgttgaa agaatccgag cgcgctattt ccgaggccaa tcgcaagctg tatgagttaa
      721 accgccagca gcgtgaaata aattcctcgc tggccacgct gcaaacacaa agccggcaga
      781 ctgaaagcaa cttgcgcaac cagcaagcgc ttctggaaaa actgctctac caggcttaca
      841 tcgggcagca gccggactac ctggccctgc ttttaaacgg ccaggacccc gaccggctgg
      901 cgcggcagct ccattactac agctatattt cccgcgcgcg cgctgattta atcgccaggc
      961 tgcggggcaa tctcaaccag ctcgccaccc tctccgccca aaccagggaa aaaagcgacg
     1021 agctcaaagc catccaacgg gaacatgcag cgcagaagcg gcaattggaa aaggaaaagg
     1081 cggaacatca gcgcgtgctg gccaggcttt cccagcaaat caccaagcaa cggcacgaga
     1141 tcggcacgct tcagcgcaac gagaaacgcc tgacccggct catcgaaagg ctggcgaaaa
     1201 tcctttcgga aaaaaaagca tcctccagac tcagcaacag ccggcttccc ggcccctccc
     1261 ttgatgccag tccttttcag caacttaagg gcaagctgaa tttaccggtg aagggggaac
     1321 tcgccaatcg tttcggcagt ccacgttctg acagcgggct tacctggaaa gggctgttca
     1381 tcctctcccc cggtgggcag gaagtgaaag ccgtcgccgc cgggcgggta gtgtacgccg
     1441 actggctgcg cggattcggc aacttgatca tcctcgacca tggcgcgggc tacatgagcc
     1501 tctacggcaa taacgaaacc ctgtacaagc aggtgggcga cgaaatccac ggcggagaca
     1561 ccatcgcggc ggtcggaaac agcggcggca atcccgattc cggtttatac tttgagatgc
     1621 ggtaccaggg caaaccgttc gaccccctgg cctgggtcac cgtcaaatag gcaaacaagt
     1681 cactggaaag cgacacgagg agatctaaat gcgggacaaa ttacagcagc ttggcctggt
     1741 aatgctgggc gccttgctgg gcgtggcttt gagcctcaac ttctccgcag tggcgcagaa
     1801 agaagcggcc gcgccgctgc cggtcgagga gttgcgggcc ttcaccgaag tgttcggccg
     1861 catcaagaac gactacgtcg aaccggtgga tgacaagaag ctcatcaccg aggccattaa
     1921 cggcatgctg accggccttg acccgcattc ggcgtatctc gatgccgatg ccttcaagga
     1981 actgcaagtc ggaacccagg gcgaattcgg cggtttgggg atagaagtcg gcatggaaga
     2041 cggttttgtc aaagtggttt ccccgattga agatacccct gcttacaacg ccgggctgag
     2101 gagcggcgac ctcatcatca agcttgatga caccgcggta aaaggcatga ccctcaacga
     2161 tgcagtgaag cgcatgcgcg gcaaacccaa tacgcaaatt acgctcaccg tggtgcgcaa
     2221 gggcgagtcc aagccgctgg tgtttacgct gacacgcgcc gtcatcaagg tgcaaagcgt
     2281 caaatccaag atgttcgagc ctggctacgg ctacatccgc atcacccagt tccaggagca
     2341 caccggcgag acgctggtta aggcaatcaa cgatttatat aagcccggca cattgaaagg
     2401 cctggttctg gatttgcgca acgacccggg cgggctgctc aacagcgccg tcgccgtgtc
     2461 cgccgcgttc ctgcaaccca atgcgctggt ggtttacacc gaaggccgca ccgatgacgc
     2521 gaagatgcgg ctttccgcca tcccggaaaa ttacctgcgc ggcaatttca aggaagatta
     2581 cctgaaaaaa ctgccggcgg cggtaaagag cgtgccgctg gtcgtgctgg tgaacaacgg
     2641 ctcggcctcg gcttccgaaa tcgtcgccgg cgcgctgcag gaccacaagc gcgccatcat
     2701 catgggcaca caaagcttcg gcaagggctc ggtgcaaacc atcctgccgc tcggcaacaa
     2761 cacggcgatc aagctcacca cggcgcgcta ttacacgcca aatggccgct cgatccaggc
     2821 caagggcatc gtgcctgaca tcatggtgga agaagccacg gtgaatggcg tcgaaccggg
     2881 cccgcggctg cgcgaagcgg atttggagcg gcatctttcc aacgatacgc agccggaaga
     2941 gaatcccgct ccgccaaagg ccacgccgcc caagctgaac cccaaaccca aaggcgaaga
     3001 aggcaaggtc gagcttccgg aattcggcgg gaaaaacgat taccagctga accaggcggt
     3061 caacctgctc aaaggcctgc aaatcctgca agggcaaaaa tagctctcac caaccgctga
     3121 atgccgaggt aaaacctctg caaattgatg gagtaagacg gcccagcgag gcaaaaattg
     3181 cgagcaaagc ggagtttaca caattagtaa atgagcattg gcgagcaatt tttaacaaag
     3241 ctggggcggc ttagacgcaa tttgcaatga atgacaatca gctaatcagg tacagccggc
     3301 acattctgct gccgcaaatc ggcattgaag gccaggaaaa gctcctccac tcgcatgcgc
     3361 tgattatcgg cgcaggcggt ctgggctccc ctgccgcgct ttatctcgcg gcgggcgggg
     3421 tcggcaaaat caccatctgc gatggcgaaa aggtggacct caccaacctg caacggcaaa
     3481 tcgtccacca cacactttct gttggcacac ccaaaaccca atccgcgcgc aacacgcttg
     3541 gactcatcaa tcccgaagta actgtggtcg aggttcccaa gcgcgtaacc cgcgaagaca
     3601 tccttgattt ggtaaggagc gccgatgtgg tgctcgatgc cagcgacaac ttcgccaccc
     3661 gtcatgcagt gaacagcgcc tgcgtcgcac accgcaagcc tttggtttca ggcgccggcg
     3721 tgcgcttcga cggtcaagtc gcggtgttcg atttgcggga aacttcgagc ccctgctacc
     3781 actgcctgtt ccccgaagaa ggcgaagtcg aggaaacgcg ctgcgcggtg atgggcgtgt
     3841 tcgcaccgtt aaccggcatc atcggcacta ttcaggcggc ggaagcgctg aagcttttaa
     3901 ccgacacggg cgaaacgctg aacggaagat tgcttttgct tgatgcgctt tcaatggaat
     3961 ggcgctcgat caaactcgcc aaagacccgc aatgttcggt ttgcggcagg aaagcaaatg
     4021 cggtgaatgc ttaacgcgta aattcggcgg cgcttcagtg cgtccactgg aatggcgggt
     4081 taggcataac tttccaactc ttttaatgca cgatatttcc cacggtggtt tgcgaaacgc
     4141 caaattcgcg agcaagtttt gcctgagaga taccaccttt agaacccata atcggatcga
     4201 gccctcttat ccgaaaacct aacctcgcat caattcccgc cactgcccgg cccagtgctc
     4261 catcggttcg cgcttgaaac cgctcttggc gtattggtgc cagcggccgg tgacgaagca
     4321 tattaaaaga ttggcctgcg ccgccgtgtc cagatcggcg ggaatttcac tgcggctcgc
     4381 ggcaaagcgc agcgcctggc gcagcgaggc ttcgagccgg tcgtgcaatt gattgatgcg
     4441 ggtttgcagc cgttcattct cgttcaccag cgcatcgcct atcagcacgc gggtcatccc
     4501 cggatttttc tcggcgaaac ccaggagcat cgagagcatc gcttccagct gcttcaagcc
     4561 gctttgttct tccgaggtga ttttgttaat cagcccaaac agcgattctt cgacgaattc
     4621 aatcagcccc tcgaacatct gcgccttgct ggcgaaatgg cgataaagcg ccgcttcgga
     4681 aatgtcgagt ttcgccgcca gcgccgcagt agttattttc tccgcacgag ggttttgcag
     4741 catttgcgcg agcgtctgca aaatctgcat cttgcgctcg cctgtcttag cggccattgc
     4801 tttgcctcag attaaagttc ccaagcagcc tcagtaattc catcacgctc tggatattca
     4861 tattgacgta ggagggagct ttgagtgaag tgctgaccca tatggttttc atgccgagct
     4921 tcttcgctgt tttgaggttt tccaacgtat cgtcaatcat cacgcaacgc tgcgggcgtg
     4981 ccccggttct gctgcacaaa cgcagaaaac cg
//
LOCUS       DATIOW010000180         2332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_181346, whole genome shotgun sequence.
ACCESSION   DATIOW010000180 DATIOW010000000
VERSION     DATIOW010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181346"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /gene="gshB"
                     /locus_tag="VLL03_06045"
     CDS             <1..636
                     /gene="gshB"
                     /locus_tag="VLL03_06045"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HSS46957.1"
                     /translation="AETQGAKVFNRPRALRDYNEKLAIAKFNTFTVPSLVTRRAELIL
                     KFLTQHQDVILKPLDGMAGRSVFRVREQDVNLNATIETLTQHGTRTIMSQRYIPEIVK
                     GDKRILLIDGKPVPYALARIPKPGETRANLATGGEGRAQPLSRRDREIAEALGPELRK
                     EGLILVGLDVIGDYLTEINVTSPTCMREIADQTGFNVAGMMIDALERAVKE"
     gene            640..1710
                     /locus_tag="VLL03_06050"
     CDS             640..1710
                     /locus_tag="VLL03_06050"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HSS46958.1"
                     /translation="MKSEELKLATPRYLPFTIYCLLFFGLAGCGKEPFYQEQSFVFGT
                     LVEVSIYGEDEARAKQLAQQVLASFDRLHYMLHAWKPDELTRINAVLAKNPQRTAITP
                     EMAAIIRHATEVAEQSGELFNPAIGKLVGLWGFHSDEVLPANPDPGEIARIVSAKPKM
                     SDVVVEGEEIYGKNPEVQLDFGGYAKGYALDDAAAYLRAQGVKNALINIGGNIMALGK
                     HGDRAWRVGIQHPRQPSAIATLELKDGEAIGTSGDYQRYFMRDGKRYCHIIDPRTGFP
                     VQGVQAVTVVIAPGVHAGALSDATSKPPFIEGVAGWRGAAKKLGVELAMLIDERGEVY
                     VTQALKQRLEFQGKSLLIHEIP"
     gene            1784..2197
                     /locus_tag="VLL03_06055"
     CDS             1784..2197
                     /locus_tag="VLL03_06055"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46959.1"
                     /translation="MVGILIVAHGTLGESLIHCASHVLGSRPLNVMQIGVTVHDDPQA
                     ILPQALKLTKQLDQGKGVLVLSDIYGATPCNIAAKLLVPGKVEGVAGVNLPMLIRALT
                     YRDEPLKTVVKKALSGGCEGVLHMNIDVCNAATGS"
     gene            2178..>2332
                     /locus_tag="VLL03_06060"
     CDS             2178..>2332
                     /locus_tag="VLL03_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HSS46960.1"
                     /translation="MRQREAEIVNKLGLHARAAARLTQLAGKFKSEIWLSRNGRRVNP
                     KSIMGVM"
BASE COUNT          543 a          654 c          700 g          435 t
ORIGIN      
        1 gcggagacgc agggtgcaaa agtgttcaac cggccgcggg cgctgcgcga ttacaacgaa
       61 aaactcgcca tcgccaaatt caatacattt accgtgccga gcttggtgac gcgccgggcc
      121 gaactgattt tgaaattcct gacacagcat caagacgtga ttctcaaacc gctcgacgga
      181 atggcgggac gatcggtgtt ccgcgtacgc gaacaggatg tgaatctcaa cgccaccatc
      241 gaaacgctga cgcagcatgg aacacggacc atcatgtcgc aacgctacat cccggaaatc
      301 gtcaaaggcg acaagcgcat cctgctgatt gacggcaagc cggtgccgta cgcactggcg
      361 cgcatcccga agccgggcga gacccgcgct aatctcgcca ccggcggtga aggcagggcg
      421 cagccgctct ccaggcgcga ccgggaaatc gccgaagcgc tcggtccgga gctgcgcaaa
      481 gaaggcttga ttctggtcgg cctcgacgtg attggcgatt acctcaccga aatcaatgtc
      541 accagcccca cctgcatgcg cgaaatcgcc gatcagaccg gctttaacgt cgccggcatg
      601 atgattgacg ccctggaaag agcagtgaaa gagtgaaagg tgaagagtga agagttgaaa
      661 ctcgcgacgc ctcgctattt accatttact atttactgtt tactgttttt tggccttgcc
      721 ggctgcggca aggaaccgtt ctaccaggaa caatccttcg tttttggaac tctggttgaa
      781 gtcagcattt acggcgagga tgaagcgcgg gcgaagcaat tagcacagca ggtactcgcc
      841 agttttgacc ggctgcatta catgctgcac gcctggaagc cggacgaact cacgcgcatc
      901 aacgccgttc tcgctaaaaa cccgcagcga acggcgatta cgccggagat ggccgccatt
      961 atccgccatg cgaccgaagt ggccgagcaa tcgggcgaac tcttcaatcc ggcaatcggc
     1021 aaactcgttg gcctgtgggg ttttcacagc gatgaggttc ttcccgccaa tcccgatccc
     1081 ggggaaattg cacgcatcgt gtcggccaag ccgaagatga gcgatgtggt ggttgaaggc
     1141 gaagagattt acggcaaaaa ccctgaagta cagctggatt tcggcggcta cgccaagggc
     1201 tatgcgctcg acgacgccgc cgcctatctg cgcgcgcaag gggtaaaaaa cgcgctcatc
     1261 aacatcggcg gcaacatcat ggcgctgggc aagcacgggg atcgcgcctg gcgggtcggc
     1321 atccagcatc cgcgccagcc gtcggcgata gccacactgg aacttaagga cggcgaagca
     1381 atcggcacct cgggcgatta tcagcgctac tttatgcgcg acggcaagcg ttattgccac
     1441 atcatcgacc cgcgcacggg ctttccggtc cagggcgtgc aggcagtaac cgtggtgatt
     1501 gctccgggag tacatgccgg agcgctgtca gacgccactt ccaagccgcc tttcatcgaa
     1561 ggcgtagctg gctggcgcgg agccgccaaa aagctcggtg tggagcttgc catgctgatc
     1621 gatgaacgcg gcgaggttta tgtcacccag gcacttaagc aacgcctcga atttcaggga
     1681 aaaagcctgt taatccacga gattccgtag ggcctgctgc tttgtcaacg gggctggagt
     1741 aaaattgtct aagttgattt gattttaaat caaggaaacc gaaatggtag gcatactgat
     1801 agtcgcgcat ggcacgctgg gcgaaagcct gattcactgc gccagccatg tgctgggcag
     1861 ccgcccgctg aacgtgatgc agatcggggt gacggtgcac gacgaccctc aggcgattct
     1921 gcctcaggcg ctgaaactca cgaaacagct cgatcagggt aagggcgtgc tggtgttgag
     1981 cgatatctac ggcgcgacgc cgtgcaatat cgcggccaaa ctgctggttc cgggaaaagt
     2041 ggaaggggtg gcgggcgtca atctgccgat gctgattcgc gcgctcacct atcgcgacga
     2101 gccgctgaaa accgtggtga aaaaggcgct ctccggcggc tgcgaaggtg ttttgcacat
     2161 gaacatcgat gtctgcaatg cggcaacggg aagctgaaat cgtcaacaag ctgggcctgc
     2221 atgcgcgcgc ggcggcgaga ctcacccagc ttgctggaaa attcaagagt gaaatctggc
     2281 tgtcgcgcaa cggccgcagg gtcaacccca aaagcatcat gggcgtgatg at
//
LOCUS       DATIOW010000181         3241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_792829, whole genome shotgun sequence.
ACCESSION   DATIOW010000181 DATIOW010000000
VERSION     DATIOW010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792829"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..615)
                     /locus_tag="VLL03_06065"
     CDS             complement(7..615)
                     /locus_tag="VLL03_06065"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HSS46961.1"
                     /translation="MPFQTFIFYFLSAILLFAALCVITVRNPVHSALFLVLAFFTSAG
                     IWLLLEAEFLAITLVLVYVGAVMVLFLFVVMMLDINLDRLREGFWKGLYIVLPLAFLL
                     VVEMWMVLGGKYFGLGEMPLPESHASGYSNTRELGRLIYTQYVYPFEVAGVILLVAIV
                     AAIALTLRRRKDNKYQNPAKQVAVRRQDRIRIVSMPSEKRKE"
     gene            complement(910..1398)
                     /gene="nuoI"
                     /locus_tag="VLL03_06070"
     CDS             complement(910..1398)
                     /gene="nuoI"
                     /locus_tag="VLL03_06070"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HSS46962.1"
                     /translation="MKRIVEFFRPFLLFELVQGMWLTGRHLFKRKITVQFPEEKTPQS
                     PRFRGLHALRRYPNGEERCIACKLCEAVCPALAITIESEQRSDGSRRTTRYDIDLTKC
                     IFCGLCEESCPVDSIVETRILEYHGEKRGDLIYTKEMLLAVGDKYEEQIARDRAADAA
                     YR"
     gene            complement(1403..2467)
                     /gene="nuoH"
                     /locus_tag="VLL03_06075"
     CDS             complement(1403..2467)
                     /gene="nuoH"
                     /locus_tag="VLL03_06075"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HSS46963.1"
                     /translation="MSSLHELFGPVWWPYVITFSKTLGLIIAIVVPLLLSVAYLTLAE
                     RKVIGYMQVRLGPNRVTFFGIPWLGGWAQPIADALKLLTKEIIIPSGANRFLFVLAPV
                     LSIMPAMAAWAVVPFQPELVLANVDAGLLYIMAITSMSVYGVIIAGWASNSKYAFIGA
                     MRSAAQIVSYEIAMGFALVGVLMASHSLNLVEIVKAQQGGAGFLNWFFLPLFPLFLVY
                     LISGVAETNRAPFDVAEGESEIVAGFHVEYSGMAFAVFFLAEYANIILVSTMCATMFL
                     GGWLPPFNFAPFTWLPGIFWLVFKVVFIVFCFLWFRATFPRYRYDQIMRLGWKVFIPV
                     TLVWIFVIGGLQQTRFWFWG"
     gene            complement(2464..>3241)
                     /locus_tag="VLL03_06080"
     CDS             complement(2464..>3241)
                     /locus_tag="VLL03_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSS46964.1"
                     /translation="GTPAGKHAKAMLEKPLKAYLLLNTEVELDCHNPRQAVAAMRNAE
                     LVVAMSAYRHKAVEYAHVLLPIAPFTETAGTFINTEGRAQSFNSTVKPLAEARPAWKV
                     LRVLGNLLGLAGFEQESIEQVRAEILPNSDTDLSQRLDNRLRRQIFAGIETPETGGLQ
                     RIGEVPIYSADAIVRRAGSLQKTRDAGAPAAWMPSAIFERLKLREGDQVRLTQEQGSA
                     MLVAHRDDTLPQNCVRVAGAHPLTAELGAMFGEITVERAA"
BASE COUNT          752 a          969 c          872 g          648 t
ORIGIN      
        1 cactccttac tcctttcgtt tttcagacgg catggaaacg atgcgtatcc ggtcctggcg
       61 ccgcactgcc acctgctttg ccgggttttg atacttgttg tccttgcgcc ggcgcaaggt
      121 gagcgcgatg gcggcaacta ttgccaccag cagaatcacc ccggcaactt caaacggata
      181 aacatactgc gtgtaaatca gccgccccag ctccctggtg ttgctgtagc ccgaagcatg
      241 cgactcaggc aaaggcattt cacccaggcc gaaatattta ccgcccagca ccatccacat
      301 ttcaactacc agcaaaaacg ccagcggcag gacgatgtat aaccctttcc agaagccttc
      361 gcgcaagcgg tcgagattga tgtcgagcat catcaccacg aacaggaaca gcaccatcac
      421 cgcgccgacg tagaccagca ccagtgtgat ggcgagaaac tccgcttcca ggagcagcca
      481 tatgcccgcc gaagtgaaaa acgccagcac cagaaacagc gccgaatgca ccgggttgcg
      541 caccgtgatt acgcacagcg ctgcgaacag cagaatcgcc gacaaaaaat aaaaaatgaa
      601 tgtttgaaat ggcatgttta attttttacc gcagagacgc agaggtgcag agaataaaat
      661 tcattttgta ttatcctttg tcataaatcg cttttgtttt tcctccgcgt cctcagcgtc
      721 ctccgcggta aattgctttt atctataaaa tctttcaccg cagagggcgc agaggagaac
      781 cttgaaaatt cttaatcaaa ggagtccgca gagaaaactc gccgggattc gtctaactct
      841 ccatgttctc tgtgacttat gcttttgttt ttcctccgca ctctcagcgt cctccgcggt
      901 aaattgttct tatctatacg ccgcatcggc ggcgcggtcg cgggcgatct gttcttcgta
      961 cttgtcccct accgccagca gcatttcctt ggtgtagatc aaatctccgc gtttttcgcc
     1021 gtggtattcg aggatgcgcg tctccacgat cgaatccacc ggacaggatt cctcgcacag
     1081 gccgcagaag atgcacttgg tcaaatcaat gtcgtagcgc gtggtgcggc gcgagccgtc
     1141 cgaccgctgc tccgattcga tggtaatcgc cagcgccggg cacaccgcct cgcacagctt
     1201 gcaggcgatg cagcgctctt cgccgttggg gtaacgacgc agtgcgtgca gacccctgaa
     1261 gcgcgggctc tgcggcgttt tctcctcggg aaactgcacg gttatcttgc gcttaaacag
     1321 gtgcctgccg gtgagccaca tgccttgcac cagctcaaac agcagaaagg gtctgaagaa
     1381 ttccacgatg cgtttcatat cgtcaccccc agaaccagaa gcgcgtctgc tgcaggccgc
     1441 cgatcacgaa aatccacacc agcgtgacgg ggataaatac tttccagccc aggcgcatga
     1501 tctggtcgta gcggtagcgc gggaaagtgg cgcggaacca cagaaagcaa aacacgatga
     1561 agaccacctt gaataccagc cagaaaatgc cgggtagcca agtgaacggc gcaaaattaa
     1621 acggcggcag ccagccgccg agaaacatgg tcgcgcacat ggtggagacc aggattatgt
     1681 tggcgtattc ggcgaggaaa aacactgcga acgccatacc ggaatactcg acatggaagc
     1741 cggcgacgat ttccgattcg ccttccgcca cgtcaaaagg cgcgcggttg gtttcagcca
     1801 cgcctgaaat cagatagacg agaaacaacg gaaaaagtgg caggaaaaac cagtttaaaa
     1861 aacccgcgcc accctgctgg gcctttacga tttcgaccag attgaggcta tgcgaagcca
     1921 tcagcactcc caccagggca aatcccatgg cgatttcgta tgagacgatt tgcgccgccg
     1981 agcgcatcgc gccgataaat gcgtatttgg agttggatgc ccagcctgcg atgatcacgc
     2041 cataaacgct catggacgtt atcgccatga tgtagagcag acctgcatca acgttggcga
     2101 gcaccaattc gggttgaaac ggcaccaccg cccatgccgc cattgccggc atgatggaga
     2161 gcaccggcgc cagcacgaac agaaagcggt tggcgccgct cgggatgatg atttccttgg
     2221 tgagcagctt gagcgcatcg gcaatcggct gagcccatcc accgagccac gggattccaa
     2281 agaaagtcac acgattcggg ccgagccgta cctgcatgta accaatcacc ttgcgctctg
     2341 cgagcgtcaa gtatgcgacg ctcaacaaaa gcggcacaac gatggcgatg ataagcccca
     2401 gcgtcttgga gaaggtgatg acatacggcc accataccgg cccgaacaat tcatgcagcg
     2461 agctcatgcc gcgcgctcca cggtgatttc gccgaacatg gcgcccagct ccgcagtcag
     2521 cgggtgcgcg ccggctactc gcacgcaatt ctgcggcagc gtgtcgtcgc ggtgggcgac
     2581 aagcatcgcc gagccctgct cctgggtcaa tctcacctgg tcgccctcgc gcagcttgag
     2641 ccgctcgaaa atcgcgctgg gcatccacgc cgccggagcc ccggcgtcgc gagttttctg
     2701 caacgacccg gcgcggcgca cgatggcatc ggctgaataa atcggcactt ctccaatgcg
     2761 ctgcaaaccg cccgtctcag gggtttcaat gccggcgaaa atctgccggc gcaagcggtt
     2821 gtcaagccgc tgcgataaat ccgtatcaga attgggaagg atttccgcac gcacctgctc
     2881 gatgctctcc tgttcgaagc cggccagtcc cagtaaattg ccaagcacgc gcaacacttt
     2941 ccatgccggg cgggcttcag cgagcggctt caccgtgctg ttgaagcttt gcgccctgcc
     3001 ttcagtattg atgaaagtgc ctgcggtttc ggtgaacggc gcaatcggca acagcacatg
     3061 cgcatattcg acggctttgt gccggtaggc gctcatggcc accaccagct cggcgttgcg
     3121 catcgcggca accgcctggc gcggattgtg gcaatcaagt tcgacttcgg tattgagcag
     3181 cagataagct ttgagtggct tttcaagcat cgccttggca tgcttgccgg ccggcgtccc
     3241 g
//
LOCUS       DATIOW010000182        12834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1502580, whole genome shotgun sequence.
ACCESSION   DATIOW010000182 DATIOW010000000
VERSION     DATIOW010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12834
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502580"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..1743
                     /locus_tag="VLL03_06085"
     CDS             307..1743
                     /locus_tag="VLL03_06085"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSS46965.1"
                     /translation="MVTQAKPDLLIVDDDPLITDTLNFVLSKDFSVYAADSRNQVKSL
                     LRQLEKPPQLALIDLGLPPTPHRPDEGFRLINELLAHSPGIKIVVLSGQNDEANARHA
                     RTLGAIEFIPKPCEPEKLKSLLTKALEIRDAELGVAPADSGIIGSSSVIEKLRNQIGQ
                     YADAPFPVLIEGESGSGKELVAASLHRLSSRRQKPYLALNCAAISPNLVEPTLFGYSK
                     GAFTGADASRQGYFEDARDGTLFLDEIGELPLELQAKLLRVLENGEYQRVGETQSRTS
                     NARVVAATNRDLRQEIKAGRFRSDLYHRLSVFSISVPPLRDLGEDKRALLDYFCDFYA
                     RQSQVTPFTLDDKSLLLWLDYPFTGNVRELRNIAIRLTTKYPGQTVGTAQLQSELDMD
                     GMEVKTPEPVVSQDIKFLQEAAKKHLQNQKNFNLDQMLQEWERSYVEAALSITHGNLS
                     QAAKLLGVRRTTLYSRMQQYGNLEENKE"
     gene            1756..3273
                     /locus_tag="VLL03_06090"
     CDS             1756..3273
                     /locus_tag="VLL03_06090"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSS46966.1"
                     /translation="MYYDHFGLTQPPFKITPNTEFFFSGGNRGAILDALIYAISHGEG
                     LIKVSGEVGSGKTMLCRMLQARLPAHIESVYLANPSVSPEEVLHVVAFELQLPVPNQA
                     PRLEVMQALHHYLLERHAQKRQVVVFVEESQSMPLATLEEMRLLSNLETGSHKLLQII
                     LFGQPEFDEILRRPEIRPLRERIIYSFSLWPLNDQQIKDYLMFRMRAAGYRGPDLFSP
                     GVVRMIAKASKGLTRRVNLLADKALLAAFADNTHTIKPKHIKAAIKDSEFGSDTGRRR
                     PVPGVAWLAFGIALGALLYGAYLSLWLPFEAPSNNQPAASAAPVPQNAPAKPAASEGP
                     KPAAAEPAVPGSSQASVQVGAQKTGPGELGLPLRMPPTVMLAPASAEGKPEKPAQAEA
                     DAAFVQYPLLQQRIEAAQNAFARAAAENFSIQLFLTEDLRPPRIERFLTRAEKLVNLD
                     EIYVYPVKTGGQVRFRVFYGLYPSQQEARVAMGQLPAKYSEVFKLKLFKIEEFQN"
     gene            3451..5250
                     /locus_tag="VLL03_06095"
     CDS             3451..5250
                     /locus_tag="VLL03_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HSS46967.1"
                     /translation="MQHLCLLRFFFFLSLALLFGGCAANYPHIPQSQGHISAAKPAIE
                     PAEIPAPVTTTAFVPPPKPVEKPQTYSVVVNEVPAKELLFALGRDTKQNIDIHPAIQG
                     LVTLNAVNETLPAILDRIARQVNIRYKLEGDTLVVSPDMPYFKTYKVDYVNLTRNTTS
                     AIGISGLISTPVTGGAGGGAQASSGSSSAGNSSTTNVSTTSNNNFWEVLKDNIRSILS
                     TTKVQTLSAEDKATRAEALKAAREERLQQAEAVSRAGQAAPTLYNAAFGGGSPAIPGD
                     VKDDVVVNPVTGTVSVLASEKQHALIKQYLDGVIESSQRQVLIEATIVEVTLTDQYQA
                     GVDFTRLGNFTIKQSLLGTNLGTPPFTQIGYAVGGNTNITSTIKLLEQFGKTRVLSSP
                     KLMALNNQTALLKVVDNIVYFSIQAQQGASSSTGVFQPTTFNTTAQTVPVGVVMSVTP
                     QINENGAVSLTVRPTISRITKFVNDPNPSLIVVTPTGVPGLNTTTTITNPVPQIQTRE
                     MESVLQVNSGQTIILGGLMQDEEIRSRDAVPGLGQIPLFGDLFSFRNDSLTKTELLIF
                     LRPTVVANPSLDSDELKFFKRFLPQPEISGSTQ"
     gene            5247..6371
                     /locus_tag="VLL03_06100"
     CDS             5247..6371
                     /locus_tag="VLL03_06100"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSS46968.1"
                     /translation="MSLLMKALQKAAQNREGGSQGADEVTEKDSSELKLQPMESEPTA
                     AWAEPPATASPVMAATVMRVGETPGGFGNYIRGHPMVLFGLIAALLVIGGFIYVLLTI
                     NKPALFTAPAQPLAKAPLAVALAPQTAPPSVQPVPESKLLFTDLPPEPREQGEVNAAR
                     APRPAAPRALPQQNISVSPVRAAPTVDPQLQDAYSALQQNRLETAQTAYQKFLSAEPN
                     NVEALLGLAAIAEQQKQPDQAAQYYMKALQTDPKNATAQTALIGLVGRADPQASEARL
                     RQLLAREPSAFLYFTLGNLYAGQSQWPGAQDAYFQAHHLQSDNPDYAFNLAVSLEHLN
                     QPKPALKFYQQALVLARNKGQAHFDLNLAQNRITRLSSLE"
     gene            6378..8075
                     /locus_tag="VLL03_06105"
     CDS             6378..8075
                     /locus_tag="VLL03_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HSS46969.1"
                     /translation="MEQRKKMRLGEMLIQQGLVTADQLRIALTEQKQSKMQVGRQLVK
                     LGFVSEAVIRDLMARTIGQESIDLSHVVADPEALKMVPQEFARRYRMLAIAFDAENKI
                     LTVAVSEIFNVVALDQLRATLGPHIEIKTLLAGEAQLEESIDRFYGFELSVDGILQEI
                     ETGEIDYQSLQAAGDEYTQPIVRLVDALLVDAVKRGASDIHFEPEYAFLRIRYRVDGV
                     LEQVRSLHKTYWPGIAVRLKVISEMNIAETRSPQDGRLSLNLNGREVDFRVSTQPTVY
                     GENIVLRVLDREKSIINLERMNLPKEIVRKMSFMLSRPEGILILTGPTGSGKTTMLYS
                     LLSKLNNEEVNIMTLEDPVEYPVTLMRQTSVNEAARMDFANGIRSILRQDPDIILVGE
                     IRDKETAEMAFRAAMTGHQVFTTLHTNSALGAFPRLMDIGILPDIIAGNVIGVVAQRL
                     VRLLCPKCKKAVDPTSLERKMFKLEDTSLKIYKPAGCKHCNNKGYKGRTAVVEVLQMD
                     SDLDELVARRATLRELQVAALAKGFNTLANEAARLVLEGTTSSAEVTRTVNLTDQVN"
     gene            8119..9324
                     /locus_tag="VLL03_06110"
     CDS             8119..9324
                     /locus_tag="VLL03_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSS46970.1"
                     /translation="MSTFQYKAVDRAGRPARGSLDAINEVDLELRLKRMGLDLITFRQ
                     INRSASALARGKITRRDLITFCLDMEQISRAGIPLLEGMHDLRDTLENPRFREIITSL
                     LEDMEGGKQLSQALAAHPHVFNHVFVSLIKAGEQAGRLTEVFESLATTIKWQDELIGQ
                     TRRLMLYPILVLLVILVVMVMLLVVLVPQVVSLLKNMGLPLPLQTRVLIFISNSIIHY
                     WPYMLGVPLFSGIVFGYLVRQNLKMRYFWDYLKLHLPITGEIMQKIIMARFSNFFALM
                     YQSGITILEAIKTSEGIVANRVIVDGLQRAGQQIGSGESLTEAFRNLGMFPPLVIRML
                     RVGEATGALDTALLNVSYFYNRDVKEAVDKGLKLLGPLTTIILASLLLSIMTATLLPV
                     YDILGKLKF"
     gene            9333..10880
                     /locus_tag="VLL03_06115"
     CDS             9333..10880
                     /locus_tag="VLL03_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46971.1"
                     /translation="MADKIFLCVSASQATAGHWRGGKLVSVQVFANDQNGWSGFNGLL
                     QSSPGAPVHLMVDTVEEDYRFENLPHSFGSDRREMIARKLRQLYRNNPYSRAWLQGRE
                     PDKRRDDRFLFAALTNQDFIAGWLKVIEAQQAPLAGIYLLPMASHNLLKKFGLDNTSN
                     LLLVTQNSAGLRQTFFHDQRLRISRLTPLETISNESRITEYAEEIGNTRLYLAAVKVI
                     PLEEPLLVVILDQSETLAGLSRELVKRQSNVLCQNFGRSEIVAKLGVAPELLQLSPDV
                     LYLHMLGELQPDINLAPEEKTRRFWRYAASRRLYAASIAVALIALLWCGANLYQQFDY
                     KEEAARALQQASQQQVLYLEVTKQFPAAPTSAENLRNAVEIAQKIKQSAHSPELMFAA
                     ISHVLDASPGVVLKSVSWKYGTESLEAEPDQTRQPSAASGPKKQIGVIEAEIRPFKGD
                     YRAAITSINSFVEKLRNDKAVAEAAIMQLPLNVNPGTSLSGNTTDSREQTSSAPFKVT
                     LSLRQES"
     gene            10877..11497
                     /locus_tag="VLL03_06120"
     CDS             10877..11497
                     /locus_tag="VLL03_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46972.1"
                     /translation="MKFSAEDFKALRLPLFTLLAVLFAGAALVYYTRLAVDVALRALS
                     QQQSQLRETRIRYQKSGDEKEIIVRYLGDYRRLQQHGLIGSEQRINWLDGLRLANQQA
                     ELFGVEYQIGAQQPYAQAQEFNPGNVAIRQSPMKLNFRLLHEGDLMRFFDILGAKGIG
                     LFHLNQCKLERLDNNVTSIRFQPNLRADCELTWFTLNPPESRGAKP"
     gene            11494..12099
                     /locus_tag="VLL03_06125"
     CDS             11494..12099
                     /locus_tag="VLL03_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46973.1"
                     /translation="MKWIAIPLLMLPLASASAEPMGRLFFTPAQRETLDNARRQNIKI
                     DVETEAPTLESISVNGVVKRSDGESIIWINNRPIKNQRAPGGIKITPRDTDRARVNVQ
                     FPQSGRNVDLKVGQSLDAVSGQVVESFQRPVPPAVTEKKSTAAEKPQPSPAEQPAAPM
                     EKPKSRISSRQAGDEPVSEPSAPNDEQPANPTPSQPASVQY"
     gene            12173..>12834
                     /locus_tag="VLL03_06130"
     CDS             12173..>12834
                     /locus_tag="VLL03_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46974.1"
                     /translation="MLALLLIISVGLIFGLARPSTLAINRDKQTAAALAMAKDALIGR
                     AATDTTIPGSLPCPDAVTNIPGINVPNDGIADLFAGSNCPSYIGRLPWRTLVLPDLRD
                     GSGERLWYALSPNFRDHPSAQPINSDTKGNITVYADSTAATLTNEAVAVIFAPGAALG
                     TQNRDTTTSALCPTTGTTIALNLCAANYLETTGGVNNATTNGPFIQAQSSGTFNDRLL
                     VIT"
BASE COUNT         2945 a         3920 c         3527 g         2442 t
ORIGIN      
        1 agcgacaacg gcatgccttg agcgccgccc cgtccctgcg ggttgcggcc caaaagcgcg
       61 tctgcgtgcg gccaaccctc accccatccc tctcccgtat gcgggagagg gagtgttcgt
      121 tccttctccc tttgggagaa ggttaggatg agggcggctc gggctggcgt tactacggag
      181 tagcgttacg ccctcgcgcc ttgcatccgc atcttttggg acagcaaggc aaaggtgctt
      241 tactatgtta tagagacccc aagactctgg caaaatgacc acacagcgtg caatctgagg
      301 agcaaggtgg taacccaagc caaaccggat ttgctaatcg ttgacgacga tccgttgatt
      361 accgacacgc tcaatttcgt gttgagcaag gatttttccg tctacgctgc ggattcccgc
      421 aatcaggtaa aaagcctgct gcggcagctc gagaaaccgc cgcagctcgc gctcatcgac
      481 ctcggcctgc cgcccacgcc gcaccggccc gacgaaggct ttcgcctcat caacgaactg
      541 ctcgcccatt ccccgggcat caagatcgtg gtgctgtccg ggcagaacga cgaggccaac
      601 gccagacacg cgcgcaccct gggcgcgatt gaattcatcc ccaagccgtg cgagcctgaa
      661 aaacttaaaa gcctcctcac caaggcgctt gaaatacgcg atgccgaatt gggcgtggcg
      721 cccgcggaca gcggcatcat cggctcaagc tcagtaatcg aaaaattgcg caaccaaatc
      781 ggccaatatg ccgacgcgcc gtttccagta ctgatcgaag gcgaatcggg aagcggcaag
      841 gaattggtcg ccgcgtccct gcatcgctta agcagccggc ggcagaagcc ttatctcgct
      901 ttgaactgcg cggcgatttc acccaacctg gtggaaccga cgttgttcgg ctacagcaaa
      961 ggcgccttca ccggcgccga tgcatcgcgc cagggctatt tcgaggacgc gcgcgacggc
     1021 acccttttcc tcgacgaaat cggcgaactg ccgctagaac tgcaggcgaa gcttttgcgc
     1081 gtgctggaaa acggcgaata ccagcgcgtc ggcgaaaccc aaagccgcac cagcaacgcg
     1141 cgcgtggtcg ccgccaccaa ccgcgacctg cgccaggaaa tcaaggcggg gcgcttccgt
     1201 tccgatctct atcaccgctt gagcgtgttc agcatcagcg tgccgccgct acgcgatctg
     1261 ggtgaagaca agcgcgccct gctcgactac ttctgcgatt tctatgcgcg ccagagccag
     1321 gtcaccccgt tcacgctcga cgacaagtcg cttttgctgt ggctcgacta tcccttcacc
     1381 ggcaacgtgc gggaattgcg caatatcgcg atccggctca ccaccaaata tccggggcaa
     1441 acagtgggta cggcgcaatt gcaaagcgag ctcgacatgg acgggatgga ggtgaaaacg
     1501 cccgagccgg tggtttcgca ggatataaaa ttcctgcagg aagcggcgaa aaagcattta
     1561 cagaaccaga aaaatttcaa cctcgaccag atgctgcagg agtgggaaag aagttatgtg
     1621 gaagcggcgc tctccattac ccacggcaat ctgtcgcagg cagccaagct tttgggcgtg
     1681 cgccgcacca cgctctacag ccgcatgcag caatacggca atctggaaga aaacaaagaa
     1741 taggcgcccg gcattatgta ttacgaccac ttcggcctga cacagccacc gttcaaaatc
     1801 actcccaata cggaattttt cttttccggg ggaaaccgcg gcgccatcct cgacgccttg
     1861 atttacgcca tttcgcacgg cgagggcttg atcaaggtga gcggcgaagt gggcagcggc
     1921 aagaccatgc tgtgccggat gctccaggcg cgtctacccg cccatatcga aagcgtctat
     1981 cttgccaatc ccagcgtctc gccggaagaa gtcctgcatg tcgtcgcctt tgaactgcaa
     2041 ttacccgtcc ccaaccaggc gccgcgcctc gaagtaatgc aggcactgca ccattatctg
     2101 ctggaacgcc acgcgcaaaa gcgccaggtg gtggtcttcg tcgaggaatc gcaaagcatg
     2161 ccgctcgcca cgctggagga aatgcggctt ctgtccaatc ttgaaaccgg cagccacaag
     2221 cttttgcaaa ttatcctgtt tggtcagccg gagttcgatg aaatcctgcg caggcccgag
     2281 atccgtccgc tgcgcgagcg cattatctac agcttttcgc tgtggccgct gaacgaccaa
     2341 caaattaagg attatctgat gttccgcatg cgcgccgcgg gctaccgggg cccggacctg
     2401 ttttccccgg gcgtggtacg catgatcgcc aaagcctcaa agggactcac gcgccgcgtc
     2461 aatctgcttg cagacaaagc gctgcttgcg gcattcgcgg acaatacgca taccatcaag
     2521 cccaagcata tcaaggctgc aattaaggac agtgaatttg gcagcgacac ggggcgccgt
     2581 agaccggttc ccggggtggc ctggcttgcg ttcggaatcg cactcggagc cttgttgtac
     2641 ggcgcctacc tctcgctttg gctgccattt gaagcgcctt ccaataatca gcccgctgcc
     2701 agtgccgcac cggttccgca aaacgcgccg gcaaagcctg cagcgtctga agggcccaag
     2761 ccggcagcag cagaaccagc tgtacccggt tcttcccaag ctagcgtaca ggttggcgcg
     2821 caaaaaaccg gccccggcga actcggcctc ccactgcgga tgccccctac agttatgtta
     2881 gcccccgcca gcgcagaggg caaacccgaa aagccggcgc aagccgaggc agacgccgcg
     2941 tttgtacaat atccattgct gcagcaacgc attgaagcgg cgcaaaacgc gtttgcccga
     3001 gccgcagcgg aaaatttcag catccagctg ttcctcacag aggacctgcg gccgccgcgt
     3061 atcgaacgtt ttcttacgcg cgccgagaag ctggtgaatc tagacgaaat ttacgtatac
     3121 ccggtgaaga cagggggcca agtgaggttc agggtgtttt acggtctgta tccttcacag
     3181 caggaagcta gggtggcgat gggtcaactt cccgccaaat actctgaagt attcaaactt
     3241 aaacttttta aaatagaaga atttcagaac tagtagcccg tttcccgggc atagcgcgag
     3301 tctggaataa actcctgaat gcacgggtat aaagcttgtt gctctgtata gattgccaag
     3361 aatgcttaga taagttgtaa taaaatcgtg ctaggattaa gatttatagg gaatatcgtg
     3421 tgttgcttaa cataaaggaa ccgggaaaaa atgcaacacc tctgtttatt gcgattcttt
     3481 ttttttctgt cactggcctt gctcttcggc ggctgtgctg ctaattatcc gcacatcccg
     3541 caatctcaag gccatatcag cgcggccaaa cctgcgattg agccggcgga aattcctgcg
     3601 ccggtaacaa ctaccgcctt cgttccccct cccaaaccgg tcgaaaaacc gcagacctac
     3661 agcgtggtag taaacgaggt gccggcgaag gaactcttgt ttgcactcgg gcgcgacacc
     3721 aagcaaaaca tcgacatcca ccccgcaatc caggggctgg tgacgctaaa tgcggtgaat
     3781 gaaaccctgc ccgcgatact cgaccgcatc gcgcgccagg tcaacatccg ctacaagctg
     3841 gaaggcgata cgctggtggt gtcgccggac atgccttact ttaaaaccta caaagtcgat
     3901 tatgttaacc tcactcgcaa caccacgtcc gccatcggta tttccggcct aatttctact
     3961 ccggtcaccg ggggggcagg ggggggcgct caggcttcgt ccggcagcag ttcagccggt
     4021 aattcttcca ccaccaacgt cagcaccacc tccaacaata atttctggga ggtgctgaaa
     4081 gacaatatcc gcagcatttt aagcaccacc aaggtgcaga cgctgtccgc ggaagacaag
     4141 gcgacgcgcg ccgaagcgct gaaagcggca cgcgaggagc ggctgcagca agccgaagcg
     4201 gtgtcgcgcg cgggccaagc ggcaccaact ctatacaacg ccgcttttgg cggcggttcg
     4261 ccagcgatac ccggcgatgt aaaagacgat gtcgtagtca atccggtcac cggcacggta
     4321 agcgtactgg cgtcggaaaa acagcatgca ctaattaaac aatacctcga cggtgtcatc
     4381 gaatcttcgc agcgccaagt gctgatcgaa gctaccatag tggaagtcac tctcaccgac
     4441 caatatcagg cgggagtgga tttcacccgt ctcggcaatt tcaccatcaa gcaaagcctg
     4501 ctcggcacta acctcgggac gccgccattt acccagattg gttatgctgt tggcggcaac
     4561 acgaatatca ccagcaccat caagctgctg gagcaatttg gcaaaacgcg ggtgttatcg
     4621 agcccgaaac tgatggcgct gaataaccag acggcgctgt tgaaggtggt ggataacatt
     4681 gtttactttt caatacaggc gcaacagggg gcttcgtcct caacgggtgt tttccagccg
     4741 acgacattca acactacggc acagaccgtg ccagtgggtg tggtgatgag cgtcactccg
     4801 caaatcaacg agaacggcgc ggtgtcgctt accgtgcgcc cgaccatttc gcgcatcacc
     4861 aagttcgtca acgaccctaa ccccagtctc atcgtcgtta ccccaaccgg tgtgccgggg
     4921 ctcaacacca ctactaccat caccaatccg gtgccgcaga tccagaccag ggaaatggaa
     4981 tcagtgctgc aggtcaacag cgggcagacg atcatactgg gcggtctgat gcaggatgaa
     5041 gaaattcgca gccgtgacgc agtgccgggc ttgggacaaa ttccgctttt cggtgacttg
     5101 ttcagcttcc gcaacgacag cctgaccaaa accgaactat tgatttttct gcgcccgaca
     5161 gtggtggcca atccctcatt ggacagcgat gaactgaaat tcttcaagcg ttttttgcca
     5221 caacctgaaa tctccggcag cactcaatga gcctgttgat gaaagccttg caaaaggctg
     5281 cccagaaccg tgaaggcggc agccaaggcg ccgacgaagt tacggagaag gattccagcg
     5341 agctcaagct gcaaccgatg gaatccgagc ccacggcggc ctgggccgag ccgcctgcca
     5401 ccgccagccc tgtcatggcg gcgacggtga tgcgcgtggg tgaaacaccg ggaggattcg
     5461 gcaactacat tcgcggtcat ccgatggttt tgtttggcct cattgcggct ctattagtga
     5521 tcggcgggtt tatctacgtg ctcctgacca tcaacaaacc cgccttgttc accgcgcccg
     5581 ctcagcccct ggccaaggct ccgctggctg tcgcgctcgc gccacagaca gcgccaccat
     5641 cggtccaacc cgtaccggaa tccaagctgc ttttcaccga cttgccgcct gaaccgcgcg
     5701 aacaaggcga agtcaacgct gcccgtgccc cgcggcccgc tgcgccgcgc gcactgccgc
     5761 agcagaatat cagcgtcagc cccgtccgcg ccgcaccgac agtcgatccg cagctgcagg
     5821 acgcttattc agcgctccag caaaaccgtc tcgagacggc acaaaccgct taccaaaagt
     5881 ttttgtctgc tgaaccgaac aacgtggaag ccctactcgg tcttgccgca atcgcggaac
     5941 agcagaagca gccggaccag gccgctcagt attatatgaa ggcattgcaa accgatccga
     6001 agaacgccac agcgcagaca gcgctgatcg gtttggtcgg acgcgccgac ccgcaagcga
     6061 gcgaggcgcg cctcaggcaa ttactcgctc gcgaaccctc cgcttttctg tatttcactc
     6121 tgggcaacct gtacgccggg cagtcgcagt ggcccggcgc gcaggacgcc tatttccagg
     6181 cacaccatct gcaatccgac aatccggatt atgctttcaa tctggctgtc agtctggaac
     6241 atctcaacca acccaagcct gcactcaagt tctaccaaca agcgctggtc ctggctagaa
     6301 acaagggaca ggcgcatttc gatttgaatc tggcgcaaaa ccgcattacc cgtttgtctt
     6361 cgctggagta gcggccggtg gaacagcgta aaaaaatgcg tctgggcgag atgctgatcc
     6421 agcaggggct ggtcaccgcc gaccagctgc gcatcgcgct caccgagcaa aagcaaagca
     6481 agatgcaggt cggccggcag ctggtgaagc tggggtttgt cagcgaagcg gtgattcgcg
     6541 acctcatggc ccgcaccatc ggccaggaga gcattgacct ttcccacgtc gtcgccgacc
     6601 ccgaagcgct gaagatggtg ccgcaggaat tcgcgcgccg ttaccgcatg ctggcgattg
     6661 cctttgacgc cgagaataag atcctgacgg tggcggtgag cgagatcttc aacgtggtag
     6721 cgctggatca actgcgcgcc acgctcggcc cgcacatcga aatcaaaacc ctgctcgccg
     6781 gcgaggcgca gctggaggag agcattgacc gcttctacgg cttcgagctt tcggtggatg
     6841 gcattctcca ggaaatagaa accggagaaa tcgactacca gagcctgcag gcggcaggcg
     6901 atgaatacac tcaacccatc gtgcggctgg tggacgcgct gctggtggac gcggtaaaac
     6961 gcggcgcctc cgacattcac ttcgagccgg aatacgcttt tttgcgcatc cgctaccgcg
     7021 ttgacggcgt gctggaacag gtacgcagcc tgcacaagac ctattggccg ggcatcgcgg
     7081 tgcgcctcaa ggtgatatcg gagatgaaca ttgccgaaac ccgctcgccg caggacgggc
     7141 ggctctcgct caacctcaac gggcgtgagg tggatttccg cgtctcgacc cagcccacgg
     7201 tttacggcga aaacatcgtg ctgcgggtct tggaccgtga aaaatccatc atcaacctgg
     7261 aacgcatgaa tctgcccaag gaaatcgtgc gcaagatgag tttcatgctg tcgcgtcctg
     7321 agggcatctt gatcctcacc gggcccaccg gcagcggcaa gaccaccatg ctgtattcgc
     7381 tgctctccaa actgaacaac gaagaagtca acatcatgac cctggaagac cccgtggaat
     7441 acccggtgac cctgatgcgc cagacctcgg tgaacgaagc ggcgcgaatg gatttcgcca
     7501 acggcatccg ctcgattttg cgccaggacc cggacatcat cctggtcggc gaaatccgcg
     7561 acaaggaaac agcggaaatg gctttccgcg cagcgatgac cggccatcag gtgttcacca
     7621 cgctgcacac caattccgca ttgggggcgt ttccgcggct catggacata ggcatccttc
     7681 cggacatcat cgccggcaac gtgatcggcg tagtcgcgca gcgcctggtg cggctgctct
     7741 gcccgaaatg caagaaagcc gtggacccga cttcgctcga gcgcaagatg ttcaaattgg
     7801 aagacacatc cctaaagatt tacaaaccgg ccggttgcaa acactgcaac aacaaaggtt
     7861 ataaaggccg cacggcggtg gttgaagtac tgcaaatgga cagtgacctg gatgaattgg
     7921 tggcgcgacg cgccacgctg cgcgagctgc aggttgcggc attggcgaaa ggcttcaata
     7981 cactcgccaa tgaagccgcg cgcctagtgc tcgagggcac gaccagcagc gccgaagtca
     8041 cgcgcacggt aaatctcacc gaccaggtaa actaaaagca tttcatcgca ggtaaagcag
     8101 agcccaagct aacagcccat gtccaccttt caatacaaag cggtagaccg cgccggccgc
     8161 ccggcacgcg gcagccttga cgccatcaac gaggtggatc tcgagctgcg gttgaaacgc
     8221 atgggcctgg acctgatcac cttccgccag atcaaccgca gtgcgtccgc cctggcgcgc
     8281 ggcaaaatca cccggcgcga tctcatcacg ttctgcctcg acatggagca gatttcgcgc
     8341 gccgggattc cattgttgga gggcatgcac gatctacgcg acacgctcga gaacccgcgt
     8401 ttccgcgaaa tcatcacctc gcttctggaa gacatggaag gcggcaagca gctgtcgcag
     8461 gcgcttgccg cccacccgca tgtcttcaac cacgttttcg tgagcctgat aaaagccggg
     8521 gaacaggccg gccggctcac tgaagtgttc gaaagcctcg cgacgacaat taaatggcag
     8581 gatgaactca tcggccagac gcggcgcctg atgctttacc ccatcctggt tttgctggtc
     8641 attctggtgg taatggtcat gctgctggtg gtattggtgc cgcaagtggt gtcgctgctg
     8701 aaaaacatgg gtctgcccct gccgcttcag accagggtgc tgatttttat ttccaactcc
     8761 atcattcact actggccgta tatgctcggc gtgccgctat tttccggcat cgtcttcggt
     8821 tacctggtgc ggcaaaatct caagatgcgc tatttctggg attatctaaa gctgcacctg
     8881 ccgatcaccg gcgagatcat gcaaaagatc atcatggcgc gcttctccaa tttcttcgcg
     8941 ctgatgtatc agtccggcat caccatactt gaagccatca agaccagcga gggaatcgtg
     9001 gccaaccgtg tcatcgtcga cggcctgcag cgcgccggcc agcaaatcgg ctcgggcgaa
     9061 agcctgaccg aggcattccg caatctcggc atgttcccgc cgctggtgat tcgcatgctg
     9121 cgcgtgggcg aggccaccgg cgcattggat acggcgctgc ttaacgtcag ctatttttac
     9181 aaccgcgacg tcaaggaagc ggtggacaaa ggcctcaagc tgctggggcc ccttaccacc
     9241 attatcctgg cctcgctttt gctttccatc atgactgcca ccctgcttcc ggtgtacgat
     9301 atcctgggta agcttaagtt ttaatcaacc tcatggccga caaaatcttt ctctgcgttt
     9361 ccgcttccca ggccaccgcc ggccactggc gcggaggcaa gctggtttcg gtccaggtat
     9421 tcgccaacga tcagaacggc tggtctgggt tcaatggact actgcagagc agccccgggg
     9481 cgccggtgca cctcatggtg gacacggtcg aggaagatta tcgtttcgaa aacctgccgc
     9541 acagtttcgg tagcgaccgc agggaaatga tcgcgaggaa attgcgccag ctttaccgca
     9601 ataatcccta cagccgcgcc tggctgcagg gacgcgagcc cgacaagcgg cgcgatgacc
     9661 gcttcctgtt tgccgccctt accaaccagg acttcattgc aggatggctc aaggtcatcg
     9721 aggcgcagca ggcgccattg gcgggaatct acctgctgcc gatggccagc cataatctcc
     9781 tcaaaaaatt cggcctcgac aacaccagca acctgctgct ggtaacgcaa aacagcgccg
     9841 ggctgcgcca gacctttttc cacgaccaga gactgcgtat tagccgcctt acgccgctgg
     9901 agactattag taatgaatcg cgcatcaccg aatatgccga ggaaatcggc aatacccggc
     9961 tctacctcgc ggcagtcaag gtcatacctc tggaagagcc gctgctcgtc gttatcctcg
    10021 atcagagtga aacgctggcc gggctcagca gagagctggt caaacggcaa tccaatgttc
    10081 tgtgccagaa ttttggccgc agcgagatcg tggccaaact gggagtggcg ccggagttgc
    10141 tgcaattgtc cccggacgtg ctgtatttac acatgctggg cgagcttcag ccggacatca
    10201 atctggcacc cgaggaaaaa acccggcggt tctggcgcta tgcagcgagc cgcaggttat
    10261 atgccgccag tattgcggtg gcattgatag ccctgttgtg gtgtggcgcg aacctttacc
    10321 agcaattcga ctacaaggaa gaagctgcac gggcactgca gcaggcatcg cagcaacaag
    10381 ttctttacct ggaagtgacc aagcaatttc ccgctgcgcc cacttccgcc gaaaacctga
    10441 gaaacgcggt ggagattgcg caaaaaatca agcaaagcgc gcacagtccg gaattgatgt
    10501 ttgcggcaat cagccatgta ctggatgcca gccccggcgt agtgctgaaa agcgtaagct
    10561 ggaaatacgg tacggaaagc ctggaagccg aacccgacca aacccgacaa cctagtgctg
    10621 cttcgggtcc caaaaaacag attggggtga tcgaagcgga aatccgaccg ttcaagggcg
    10681 attatcgcgc cgccatcacc agcatcaaca gctttgtgga aaaactgcgc aacgataaag
    10741 cagtggccga agccgcgatc atgcagttgc cgctcaacgt gaaccccggg accagcttga
    10801 gcggcaacac caccgacagc cgcgaacaga cttccagcgc ccccttcaag gtgactttgt
    10861 cattgaggca ggaatcatga aattcagcgc cgaagatttc aaggccctgc gcctgccact
    10921 gtttacgctg ctggccgtgc tgttcgcagg tgcggcgctg gtctattaca ccaggctcgc
    10981 tgtcgatgtc gcgctgcgcg ctctcagtca gcagcaaagc cagctgcgcg aaacgcgcat
    11041 ccgctaccag aaatccggcg atgaaaagga aatcatcgtc cgctacctcg gcgattaccg
    11101 gcgcttgcaa cagcatggat tgatcggatc ggagcagcgc atcaactggc tggatggctt
    11161 gcggctggct aatcagcagg cggagctttt cggcgtggaa taccagatcg gtgcccagca
    11221 accctatgct caggcgcagg aattcaaccc cggcaatgtg gcgattcggc aatcgccgat
    11281 gaagctcaat ttccgcctgc tgcacgaagg cgacctgatg cgcttcttcg acattctcgg
    11341 cgccaagggc atcggcctgt ttcatctcaa ccagtgcaag ctggagcgtc tggacaataa
    11401 cgtcaccagc atccgctttc agcccaacct gcgcgccgac tgcgaactca catggtttac
    11461 cctgaatcca cctgaatcca ggggtgccaa accatgaagt ggatcgcgat acccttgctg
    11521 atgctaccgc tcgcgagcgc atccgccgag ccgatgggac ggctgttttt caccccggca
    11581 cagcgcgaga cgctggacaa cgcccgaaga cagaatatca agattgatgt tgaaaccgaa
    11641 gcgccgacgc tggaaagcat cagcgtcaac ggcgtggtga aacgcagcga cggcgagtcc
    11701 atcatctgga tcaacaaccg gccgataaaa aaccagcgcg cgccaggcgg catcaaaatc
    11761 acgccgcgcg acaccgaccg cgcccgcgtc aacgtgcaat ttccgcaatc gggccgcaac
    11821 gtggatctga aagtcggcca aagcctcgat gcggtgagcg gccaggtcgt cgaaagcttc
    11881 cagcgtcccg tgccgcctgc cgttaccgag aaaaaaagca cggctgcgga aaaaccgcag
    11941 ccgagtcccg cagagcagcc ggccgctccg atggaaaaac ccaaatccag aatttcttca
    12001 cgccaagcgg gtgacgaacc ggtctcggaa ccgtccgccc ctaacgatga gcaacctgca
    12061 aaccccactc catcacagcc cgcttcggtc caatactgac ggcgctcccg cgcggcggcg
    12121 tagcgccgga ctgcacgtgt ctttcaggga aagcgggcaa gggttattct tattgctcgc
    12181 gcttcttttg attatcagcg tggggctgat attcggcctt gctcgcccta gcacgctagc
    12241 catcaacaga gacaagcaaa ccgctgcggc tctggccatg gcgaaagacg cattaattgg
    12301 acgagctgca accgacacta ctattcctgg cagtttgccc tgtcccgacg ccgtcacaaa
    12361 catacccggc atcaacgtgc caaacgatgg cattgctgac ttgttcgctg gctcgaactg
    12421 ccccagctac atcggcaggt tgccgtggcg taccctcgtc ctgcccgatt tgcgcgatgg
    12481 cagcggagaa cggctatggt atgcgctctc cccaaacttt cgcgatcatc cttcggcgca
    12541 gcccatcaac agcgacacca agggcaatat cacggtttac gcagacagca ccgcagccac
    12601 gcttaccaac gaggcggtag cggtcatctt cgcgccgggc gcggccctcg gcacgcagaa
    12661 ccgggacacg accaccagcg ccctctgtcc gacaacgggc acgacgatcg cactcaacct
    12721 gtgcgccgcg aattacctgg aaaccacagg tggggtcaac aacgcaacga ctaatgggcc
    12781 gtttatccaa gcccaaagct cgggcacctt taacgaccgc ttgctggtga ttac
//
LOCUS       DATIOW010000183         4862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1198464, whole genome shotgun sequence.
ACCESSION   DATIOW010000183 DATIOW010000000
VERSION     DATIOW010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198464"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VLL03_06135"
     CDS             complement(<1..493)
                     /locus_tag="VLL03_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HSS46975.1"
                     /translation="MEFFRIKHDIPFMRHALVFNVISLITFILAVVFLATKGLNFGVD
                     FTGGTVMEVNYAQPADIPKIREALTKLGFLDASVQNFGTSHDVLIRLQVKPGVTSAKL
                     SEQVLDALREADKTVQMRRVEFVGPQVGKELVENGSLALLLVSVGIVLYLWFRFEWKF
                     GVAA"
     gene            complement(533..895)
                     /locus_tag="VLL03_06140"
     CDS             complement(533..895)
                     /locus_tag="VLL03_06140"
                     /inference="COORDINATES: protein motif:HMM:NF008911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HSS46976.1"
                     /translation="MIKTHHDLKLWQEAMTLVKEIYQLTSAFPPEELYGLSAQLRRSA
                     VSVPSNIAEGAARSSKKEFLQFLNVARGSLSELETQVIIAKEIGYVETIDSIQSRTQN
                     LFKLIGGLVNKLKDRGND"
     gene            complement(946..2808)
                     /gene="secD"
                     /locus_tag="VLL03_06145"
     CDS             complement(946..2808)
                     /gene="secD"
                     /locus_tag="VLL03_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HSS46977.1"
                     /translation="MNRYPLWKYILIGLTLLLGFLYALPNLFGEVPAVQILPAKSALK
                     VDTTLLARVEEALTKANISAQGVFLDVNTIKARFTDTDTQLKAKDLLQAKLGDDYVVA
                     LNLLSSSPKWLSSLGALPMYLGLDLRGGVYFLLQVDMKAALDKAADRYVSDIRSTMRD
                     RKIAYTGVSRDGQTVSVKFRDAEAREKGSAEISKNFSDLALKETDEGGEPKLAATLRP
                     EAQTKFREYALQQNITTLRNRVNELGVAEPIIQQQGAERVVVQLPGVQDTAKAKDILG
                     RTASLEVRMVDEEHSDAASLEAAAKGQLPVGVELYKERNGIPVLVKKTVVLTGERISD
                     AQPGYDGQTGEPAVHLTLDGAGARIFQLLTRENVGKRMAILLIEKGKAEVVTAPVIRT
                     EIGGGRVQISGGRMSVEEARDIALLLRAGALAAPMEIIEERTVGPSLGAENIQRGFHS
                     TWIGFSAITVFMIVYYLLFGFVSVIALACNVLFLVALLSMLQATLTLPGMAGIALTVG
                     MAIDANVLINERVREELRNGTTPQAAIAAGYQRAFGTILDSNVTTLIAGVALFLFGSG
                     PVRGFAVVLCLGILTSLFSAVMVSRGIVNLIYGSRRKLDKVSIGQVWRPEGQKT"
     gene            complement(2941..3270)
                     /gene="yajC"
                     /locus_tag="VLL03_06150"
     CDS             complement(2941..3270)
                     /gene="yajC"
                     /locus_tag="VLL03_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HSS46978.1"
                     /translation="MLISQAFAQTAAPAQQGLDWVSLMPLVLMFVLLYFLMIRPQMKR
                     GKEHKTMVEALQKGDEVVTGGGVLGRITKVGDNYVTVEIAANVEIQVQKPAIQTLLPK
                     GTIKNVS"
     gene            complement(3304..4410)
                     /gene="tgt"
                     /locus_tag="VLL03_06155"
     CDS             complement(3304..4410)
                     /gene="tgt"
                     /locus_tag="VLL03_06155"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HSS46979.1"
                     /translation="MKFELKVHDSSARRGTITLAHGKVETPAFIPVGTYGAVKAMSPA
                     ELKELGADIILGNTFHLWLRPGLEVIEAHGGLHRFMGWDGPILTDSGGYQVFSLGDLR
                     KVTEKGVKFQSPVNGDACFLSPEESMRIQRVLNADIVMVFDECTPYPANEKEALKSME
                     LSLRWAERSKCAHEGNRNALFGIVQGGMYPHLREGSLRGLAAIGFDGYAIGGLSVGEP
                     KRDMQRVLKHIAPQLPDNKPRHLLGIGTPEDIVYAVGQGMDMFDCVLPTRNARNGWIF
                     TRHGTIKLRNARYRDDLRPLDEQCGCYTCRHFTRAYLHHLQRVNEILGARLNTLHNLH
                     YYQELMRGLREAVEHGRFEDFVEEFKRARLAVLE"
     gene            complement(4576..>4862)
                     /locus_tag="VLL03_06160"
     CDS             complement(4576..>4862)
                     /locus_tag="VLL03_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HSS46980.1"
                     /translation="LAVGSTSLRALEAAAAGGKLQAGSGETNIFITPGYRFRVVERLL
                     TNFHLPRSTLIMLVSAFGGMENIRKAYRHAVESRYRFFSYGDAMLIERQN"
BASE COUNT         1022 a         1472 c         1341 g         1027 t
ORIGIN      
        1 tcgccgccac accgaatttc cattcgaagc ggaaccacag gtaaagcaca atcccgaccg
       61 aaaccagcag gagcgcaagc gagccgttct ccactagttc cttgcccacc tgcggtccga
      121 caaactcgac gcgtcgcatc tgcacggtct tatccgcctc acgcagcgcg tccagcacct
      181 gctcgctcaa ttttgcgctg gtcacgcccg gcttcacctg cagacggatc agcacatcgt
      241 gcgaggtgcc gaaattctgc acgctggcgt caagaaaacc aagcttggta agcgcttcgc
      301 ggattttagg aatgtcggcg ggctgggcgt aatttacttc catcaccgtg ccaccggtga
      361 aatccacgcc gaagttgagc cccttggtcg ccagaaacac caccgccagg atgaacgtga
      421 tgagcgaaat cacgttaaac accagcgcgt ggcgcatgaa cgggatatcg tgtttgattc
      481 taaaaaattc cacgtcttta attccgtgac aagtgacgag tgacgagtga cattaatcgt
      541 tacccctgtc tttaagtttg tttactaaac cgccaatgag cttgaacagg ttctgggtcc
      601 tcgattggat ggaatcgata gtctccacgt atcctatttc ttttgcgata attacctgcg
      661 tttctaattc actcagtgaa cctctcgcaa cgttcaagaa ctgcaagaat tcctttttgg
      721 acgaacgcgc tgctccttcg gcaatgttgc tcggtaccga aaccgccgac cgccgaagct
      781 gggcggacaa tccatacagc tcttccggag gaaaagccga tgtcaactgg tatatttcct
      841 tgaccaaagt catggcctcc tgccacagct tgagatcatg atgcgtcttt atcactggtt
      901 acccgtcact cgtcacgtca cttgtcacct gttactcgtc actcgtcacg ttttttgccc
      961 ttcaggccgc cacacctggc cgatggaaac cttgtcgagc ttgcgccggc tgccgtagat
     1021 caaattcacg ataccgcgcg acaccatcac cgcgctgaat agggatgtca gtatcccgag
     1081 gcacagcacc acggcgaaac cacgcaccgg gccgctccca aacaggaaca gcgcaacgcc
     1141 ggcaatcagc gtggtgacgt tggagtcgag aatcgtgccg aatgcgcgct ggtagcccgc
     1201 ggcgatggcc gcctgcggcg tggtgccgtt tcgcagctcc tcgcggacgc gttcgttaat
     1261 cagcacgttg gcgtcaatcg ccatgcccac agtgagcgca atgccggcca tgccgggcag
     1321 tgtcagcgtc gcctgcagca tggacagcag cgccaccagg aacagcacgt tgcaggcaag
     1381 cgcgatgacg gagacgaagc cgaagagcag atagtagaca atcatgaaaa cggtgatggc
     1441 ggagaagccg atccaggtgg aatgaaagcc gcgctggatg ttttcggcgc cgagactggg
     1501 acccacggtg cgctcctcga tgatttccat cggcgccgcc aaggcgccgg cgcgcagcaa
     1561 gagcgcgatg tcgcgcgctt cctccacgct catccggccg ccgctgatct gcacgcggcc
     1621 gccgccgatt tcggtgcgga tcacgggcgc ggtcaccact tccgccttgc ctttttcaat
     1681 cagcagaatc gccatccgct tgccgacgtt ctcgcgcgtg agcaattgga aaatccgtgc
     1741 gccggcgcca tccagcgtga ggtgcaccgc cggctcgccg gtctggccgt catagcccgg
     1801 ctgcgcgtcg ctgatgcgtt cgccggtcag caccacggtt ttctttacca gcaccgggat
     1861 gccgttcctt tccttgtaaa gctcgacgcc gaccggaagc tgcccttttg ccgccgcttc
     1921 cagcgatgcc gcgtcgctat gctcctcatc caccatgcgc acttccagcg aagcagtgcg
     1981 gccaagaatg tccttggcct tggcggtatc ctgcacgccg ggcagttgca ccaccacccg
     2041 ttccgcgccc tgctgctgga tgatgggttc agcgacgccc agttcgttca cgcggttgcg
     2101 caacgtggtg atgttttgct gcagggcgta ttcccgaaat ttggtctgtg cttccggcct
     2161 aagcgttgcg gcaagtttcg gttcgccgcc ttcatctgtt tccttcagcg ccaggtcgga
     2221 aaagtttttg ctgatttcgg cggagccttt ttcgcgcgcc tcggcgtcac ggaatttcac
     2281 cgacacggtc tgtccgtcgc ggctcacgcc ggtgtaggcg attttcctgt cacgcatggt
     2341 ggagcggata tcgctgacgt agcggtcggc cgccttgtcg agcgcggctt tcatgtcgac
     2401 ttgcagcagg aagtacacgc cgccgcgcaa atccagccca aggtacatcg gcagcgcgcc
     2461 gagactggaa agccatttgg gcgagctcga aagcagattc agcgccacca cataatcgtc
     2521 accgagcttc gcttgcagga ggtccttcgc tttaagctgg gtatcggtgt cggtgaagcg
     2581 cgccttgatg gtgttgacat cgagaaaaac accttgggcg gaaatgttgg ctttggtgag
     2641 cgcctcttca acgcgcgcca gtaacgtggt atccactttc agcgcggatt tggccggcag
     2701 aatctgcacc gccggcacct cgccaaacag gttcggcagg gcgtagagaa aacccaacaa
     2761 gagggtgagg ccgatgagga tgtatttcca gagcggataa cggttcattg aaaaacggtg
     2821 acgagtgacg agtgacgagt aactagtgac cagtgaccag tgactggtga agggtgaaaa
     2881 gtgaggggtg agaaacgaca agccatcttg gttttgctat tcactgttga cggatcacaa
     2941 tcaactgacg ttttttatgg tgcccttggg cagcagcgtc tgaatcgcgg gcttctgcac
     3001 ttgaatctcg acattcgcgg caatctccac ggtcacgtaa ttatcgccga ccttggtaat
     3061 acggcccaac acgccgccgc cggtcaccac ttcgtcgcct ttttgcagcg cctcgaccat
     3121 cgtcttgtgc tccttgccgc gcttcatctg cgggcggatc atcaggaaat acagcagcac
     3181 gaacatcagc accaggggca tgagactgac ccagtccaga ccctgctgcg cgggtgccgc
     3241 ggtctgcgca aaagcttggc tgatcaacac tggtttctcc gtccagggaa taaaataaag
     3301 gcgttattct agcacggcga gacgcgcccg cttaaattcc tcgacaaaat cctcgaagcg
     3361 accgtgctcg acggcctcac gcaaaccgcg catcagttcc tggtagtaat gcaagttgtg
     3421 cagggtattg agccgcgccc cgagaatctc gttgacccgc tgcagatggt gaagataagc
     3481 acgagtgaaa tgacggcagg tatagcagcc gcattgttca tcgagcggcc gcagatcgtc
     3541 gcgataacgg gcattacgca gcttgatcgt gccgtgacgg gtgaaaatcc agccgttgcg
     3601 ggcattgcgc gtcggcaaca cgcagtcgaa catgtccatg ccctgtccca cggcgtacac
     3661 aatatcctcc ggcgtgccga ttcctagaag atgacgcggc ttattgtccg gcaattgcgg
     3721 ggcgatatgc ttaagcaccc gctgcatgtc gcgcttgggt tctcctaccg acagcccgcc
     3781 aatcgcataa ccgtcgaagc cgatagccgc caaaccgcgc agcgacccct cgcgcaagtg
     3841 tgggtacatg ccgccttgaa cgatgccgaa caatgcgttg cgattgcctt catgcgcgca
     3901 cttagagcgc tccgcccagc gtagagacaa ttccatggac ttaagcgctt ccttctcatt
     3961 cgcgggataa ggcgtgcatt catcgaacac catcacgatg tcggcgttaa gcacgcgctg
     4021 gatgcgcatg gactcttcag gcgagagaaa acaggcgtcg ccgttgaccg gcgattggaa
     4081 cttcacaccc ttttccgtca ccttgcgcaa atccccgagg ctgaacacct ggtaaccgcc
     4141 ggaatccgtc aatatcggcc cgtcccagcc catgaagcgg tgcaagccgc cgtgcgcttc
     4201 aattacctca agacccgggc gcaaccacaa gtgaaaagtg ttgcccagta tgatatcggc
     4261 acccagctct ttcagctccg ccggactcat cgccttcacc gcgccgtagg tgccgaccgg
     4321 tataaaggcc ggcgtttcca ccttgccgtg cgcaagcgtg atggtgccgc gccgcgcgga
     4381 gctgtcgtgg actttcaatt caaatttcac tcgacaaacc ctttctcacc gcagagacgc
     4441 agaggcacgg agaaaatctt ttcggtttaa agttatggat tatgagcttt gtgaattact
     4501 agcgtacctg caacacgacc agaactgaaa ataaatctgg aatttgtctt tctctgcgtc
     4561 tccgcgcctc cgcggttaat tctgtctttc aatcagcatg gcgtcgccat agctgaaaaa
     4621 gcgatagcgg ctctccaccg cgtggcggta ggccttgcgg atattttcca ttccgccaaa
     4681 agccgagacc agcataatca gagtcgagcg cggcagatgg aaattggtga gcagccgctc
     4741 caccacccga aagcggtaac ccggggtgat gaaaatgttg gtttcgccgc tgccggcttg
     4801 cagtttcccg cccgccgcgg cggcttccag cgcgcgcagg ctggtgctgc ccaccgccag
     4861 ca
//
LOCUS       DATIOW010000184         3527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1504298, whole genome shotgun sequence.
ACCESSION   DATIOW010000184 DATIOW010000000
VERSION     DATIOW010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504298"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VLL03_06165"
     CDS             <1..221
                     /locus_tag="VLL03_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HSS46981.1"
                     /translation="AQRIVSTAQSFSTWGKQTFDSLSRSFAEYWTEERPLIAKSAHVK
                     QLIADVDALRDDVERLEKRLEKLRNLQI"
     gene            358..1875
                     /gene="ubiB"
                     /locus_tag="VLL03_06170"
     CDS             358..1875
                     /gene="ubiB"
                     /locus_tag="VLL03_06170"
                     /inference="COORDINATES: protein motif:HMM:NF003404.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HSS46982.1"
                     /translation="MRLFRLLKIISISLRFGLDEFFLGHERVRALRTLIKLLLFWRTL
                     RKPRAERLRLALEALGPIFVKFGQVLSTRRDLLPTDIADELAKLQDQVPPFPSAQAVA
                     ILERVYGKPLDLVFRKFEAQPVASASVAQVHLAVLDNGTDVAVKILRPGMAAVIAKDL
                     ALLDIGAGMVETLWADGKRLKPHQVVAEFEKHLYNELDLMREAANASQLRRNFENSPL
                     LRVPEVYWDYCSTEVMVMQRMQGTPISQVAALREQGVDIPKLARAGVEIFFTQVLRDG
                     FFHADMHPGNIFVTPQGQYIALDFGIMGTLDDVDKNYLAQNFLAFFRRDYKRVAEAHI
                     ESGWIPRETRVDEFEAAIRAVCEPIFDKPLKDISFGKLLLRLFQTSRRFNVEIQPQLV
                     LLQKTLLNIEGLGRELDPDLDLWKTAKPFLERWMNEQVGWRGLISRIRKEAPYWGTLL
                     PQLPRLIHRALSEDRSQQLQQELHQLRTEHKRQNEWLAVIAALLAATLVLLVFSI"
     gene            1999..3084
                     /locus_tag="VLL03_06175"
     CDS             1999..3084
                     /locus_tag="VLL03_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSS46983.1"
                     /translation="MALLEIRNVTRRFGDLVAVDNVSINIEAGEFFTLLGPSGCGKTT
                     LLRMIAGFDLPGSGQILLDGKDLADTPPEKRPIHTVFQSYALFPHMTVTGNISFPLKM
                     AGKSPDEIAAKVKEVLELVHLSDKGESFPHELSGGQKQRVALARGLVNRPRLLMLDEP
                     LGALDAKLREEMQIELISLQRDVGITFVFVTHSQAEALALSHRIAVMNAGRIEQMDEP
                     SKIYSFPANRFVADFIGNINMLQAEVLEAASDYLRIRIPGMGEVRAPAKSGAAPGQKG
                     MLALRPEQVRIAARIDDVAAENHFPGKVRDFLYVGDITTYIVDLNNGAQIEALLPNST
                     PGRAKFFETGDTVEVAWRQDAGLFLNE"
     gene            3077..>3527
                     /locus_tag="VLL03_06180"
     CDS             3077..>3527
                     /locus_tag="VLL03_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSS46984.1"
                     /translation="MSNFLNSARITKWLVSGPPFLYLTLFFLLPSLIMVVASFRYPGD
                     YGGLAPIFGVEPGKETGLTLETYWFFFSDFIYTQIFIKSFGVALATTLVCLLMAYPLA
                     LLIAHSPKKHRDFMVLLVILPFASNFLIRVYAWMIILGPQSAFSLAVN"
BASE COUNT          722 a         1035 c         1000 g          770 t
ORIGIN      
        1 cggcgcagcg catcgttagc accgcgcaaa gtttcagcac ctggggcaaa caaacgtttg
       61 acagcttgag ccgctctttt gccgaatact ggaccgaaga acggccgctg attgcaaaat
      121 ccgcacacgt caaacaatta atcgccgatg tggatgccct gcgtgatgat gtcgagcgcc
      181 tggaaaagcg cctggaaaaa cttaggaacc tccagattta gtccggcgtg agcccattgc
      241 cggcgaaatg gggatgaagg caaggcgcga ggcgcagcca actgtcgcgg ccacgtgcga
      301 tttaatcaaa ggttccctac gctagcccga ctcgcgcctg gcggttacaa tccgcgaatg
      361 cggctgttcc gcctcctcaa aattatcagc atcagcctgc gcttcggcct cgatgagttt
      421 ttcctcgggc acgaacgcgt gcgtgccctg cgcacgctca ttaagctttt gttattctgg
      481 cgcacgctca gaaaaccccg cgccgaacgg ttgcgcctgg cgctggaagc gctggggccg
      541 atcttcgtca aattcggcca ggtgctgtcc acgcggcgcg acttgcttcc taccgatatt
      601 gcggatgagc tggcgaaact tcaggaccag gttcctcctt tcccctccgc acaagccgtt
      661 gccatcctgg agagggtgta cggcaaacct ttggatctgg tgtttaggaa attcgaggcg
      721 caacctgtgg cgagcgcatc agtcgcgcaa gtgcatctcg cggtactcga taacgggacc
      781 gatgtcgcag tcaaaatcct gcgtccaggt atggccgcgg tgattgcaaa agatttggcg
      841 ctgctggata tcggcgccgg gatggttgaa acgctgtggg cggacggcaa gcgcttgaag
      901 ccgcaccaag tggtcgcgga attcgagaaa catctttaca acgaattgga tctcatgcgc
      961 gaggcggcga acgccagcca actacggcgc aatttcgaga attccccgct actgcgggtt
     1021 cccgaagtgt actgggacta ttgctcgacc gaagtcatgg tgatgcagcg catgcagggc
     1081 acgccgatca gccaggttgc ggcattgcgc gagcagggcg tggatatccc taaactggcg
     1141 cgcgccggcg tggagatttt cttcacccag gtattgcgcg acggtttttt ccacgccgat
     1201 atgcatccgg gcaacatttt cgtcacgccg caagggcaat acatcgctct cgatttcggc
     1261 atcatgggga cgctcgacga cgtggacaag aattacctcg cgcagaattt cctcgcattt
     1321 ttccgccgcg actacaagcg ggtcgccgag gcgcatatcg aatcgggctg gattcccagg
     1381 gagacgcggg tagatgaatt cgaggccgcc attcgtgcgg tgtgcgagcc gattttcgac
     1441 aagcctctca aggacatttc cttcggcaag ctgctgttgc ggctgttcca gacttcgcgc
     1501 cgcttcaacg tggaaatcca gccgcaactg gtgctgctgc aaaagaccct ccttaatatc
     1561 gaaggtttgg ggcgcgaact cgaccccgac ctggatttat ggaaaaccgc caagcctttc
     1621 ctcgagcgct ggatgaacga gcaagttggc tggcgcgggc ttatcagccg catccgcaag
     1681 gaagcgccct attggggcac gctgttgccg caactgcctc gcctcattca ccgcgcgcta
     1741 agcgaagacc gctcgcagca actgcaacaa gaattgcatc agctgcgaac cgagcacaag
     1801 cgccaaaacg aatggctggc ggtcatcgcc gctttgcttg ccgccactct ggtcctgctt
     1861 gttttttcca tctgaaagcc ggctccgctg cggctcgccg gcagcggcgt cttgaccgga
     1921 atcatcgccg gcaattctgt tccaatcttt tgcgcatttg ctagaataag ccactttatt
     1981 taatcggctg gactctgcat ggcgcttctg gaaattcgca acgtcacccg ccgcttcggc
     2041 gatctggttg cagtggacaa cgtcagcatc aatatcgagg caggagaatt ctttaccctg
     2101 ctcggccctt cgggttgcgg caagaccacg ctgcttcgga tgatcgccgg attcgatttg
     2161 cccggcagcg gacaaatcct cctcgacggc aaggaccttg ctgacacgcc gccggagaaa
     2221 cgcccgattc ataccgtgtt tcagagttac gcactgtttc cgcacatgac cgtgacgggc
     2281 aatatctcct tcccgcttaa gatggctgga aagagccctg acgaaatcgc ggccaaggtc
     2341 aaggaagtgc tggagctggt tcatctctcg gataaaggcg aaagtttccc gcatgaactt
     2401 tccggaggcc agaaacagcg cgtggcactc gcccgcggac tggtcaaccg gccgcgcctg
     2461 ttgatgctcg atgagccgct gggcgcgctg gatgccaagc tgcgcgagga aatgcaaatc
     2521 gagctcatca gcctgcaacg cgatgtgggc attacattcg tgtttgtgac tcactcgcag
     2581 gcggaagcgc tcgcgctttc acatcgcatc gcggtgatga atgccggcag aatcgagcag
     2641 atggacgagc cttccaaaat ctacagtttt cccgcaaacc gtttcgtcgc cgacttcatt
     2701 ggcaatatca acatgctgca agccgaggtg ctggaagcgg cgtcggacta tttgcgtatc
     2761 cgcattcccg gcatgggtga agtgcgggcg cccgccaaaa gcggtgccgc gcccggccag
     2821 aaaggaatgc tggcgctgcg ccccgagcag gtacgcatcg ccgccagaat tgacgatgtt
     2881 gcggcagaaa accacttccc cggaaaagtg cgtgactttc tgtatgtggg cgacatcacc
     2941 acttatatcg ttgaccttaa caacggcgcg caaatcgaag cactgctgcc taattcgacg
     3001 ccggggcgcg ccaagttctt cgaaaccggc gataccgtcg aggtcgcctg gcgccaggat
     3061 gcaggattat tcctgaatga gtaatttttt gaattccgca cgcatcacca agtggctcgt
     3121 cagcgggccg ccgtttcttt acctgacgct gttttttctg ctgcccagcc tcatcatggt
     3181 ggtggcctct ttccgctatc ccggagacta tggcgggctt gcgccgatct tcggggtcga
     3241 accgggcaag gaaaccgggc tcacgttgga gacttattgg tttttcttca gcgatttcat
     3301 ctacactcag attttcatca aatccttcgg cgtcgccctg gccaccacgc tggtctgcct
     3361 gttgatggca tatccgctgg cgttgctgat tgcgcacagc ccgaagaagc atcgcgactt
     3421 catggtgctg ctggtgattc tccccttcgc cagcaatttc ctgatccggg tttacgcctg
     3481 gatgattatt ctcggcccgc agtcggcttt cagcctcgcg gtcaatc
//
LOCUS       DATIOW010000185         1877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_590080, whole genome shotgun sequence.
ACCESSION   DATIOW010000185 DATIOW010000000
VERSION     DATIOW010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590080"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VLL03_06185"
     CDS             complement(<1..215)
                     /locus_tag="VLL03_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46985.1"
                     /translation="MIEAQRMASFAVCQVRAGRSLTQSLLNLWQHHPSLSSLQRAHAQ
                     ELAYGTLRFYGELEQVLDALLQKPLHS"
     gene            complement(235..1080)
                     /gene="htpX"
                     /locus_tag="VLL03_06190"
     CDS             complement(235..1080)
                     /gene="htpX"
                     /locus_tag="VLL03_06190"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HSS46986.1"
                     /translation="MGNWFKTSLLMAAIMALFGVVGSLLGGGTGLLIALILGGAMNLW
                     AYWFSDKMVLRMYDARKVDESSAPKLYGIVRELAQRAELPKPKVYLIDEAQPNAFATG
                     RNPEHAAVAATTGILRLLTPRELRGVLGHELSHVKNRDILISTISASIAGAISSIAQF
                     GLLFGGGNQNERGVRPLVALLIMIVAPLAAMLIQLVISRVREFDADHDGAEISGDPEA
                     LASALEKIEHYARGQPIATAEMHPETAQMMIINPLSGGGVQGLFSTHPSTQERIARLL
                     AMARA"
     gene            complement(1152..>1877)
                     /gene="fmt"
                     /locus_tag="VLL03_06195"
     CDS             complement(1152..>1877)
                     /gene="fmt"
                     /locus_tag="VLL03_06195"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003066827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HSS46987.1"
                     /translation="EMLKELQRAVADVIVVAAYGLILPKTMLDLPRLGCINIHASLLP
                     RWRGAAPIQRALLAGDAETGVSIMQMDEGLDTGPVLMQERIPIAADDNAQSLHDKLAR
                     LGGESIVRALRALERGQLRPQPQPATGASYAAKVQKTEAQINWQGAASQIERAVRAFN
                     PQPGAYTMLNGETLKIWKTRLDYQAHGKPGEVISADDRGIVVACGSGGLRIEELQKAG
                     GKRLPAQAFLSGFAIREGARLGA"
BASE COUNT          374 a          563 c          543 g          397 t
ORIGIN      
        1 tcgctgtgca agggcttttg caaaagcgca tcgagtacct gttcaagctc gccgtaaaaa
       61 cgcaaggtcc cgtaagccag ttcctgggcg tgggcccgtt gtagcgagct caaggaggga
      121 tggtgctgcc agagattcaa caggctttga gtgaggctgc ggccagcccg cacctggcat
      181 accgcgaagc tggccatgcg ctgcgcttcg atcattgcgc gagccggcct ggatttacgc
      241 tctggccatg gccagaagac gggcaatgcg ctcctgcgtc gaagggtggg tgctgaaaag
      301 tccctgcacg ccgccgccgg aaagcgggtt gatgatcatc atctgcgcgg tctcgggatg
      361 catttcggcg gttgcgatcg gctggccgcg ggcatagtgc tcgatttttt ccaaggcgga
      421 agcaagcgcc tcggggtcac ccgaaatttc agcgccatcg tgatcggcat cgaattcgcg
      481 cacgcgggaa atcaccagct ggatcagcat cgcggccagc ggcgccacga tcataatgag
      541 cagcgccact aacggacgca cgccgcgctc attctggttg ccgcctccaa acagcaaacc
      601 gaattgggcg atagaagaaa tggcgccggc gatgctcgcc gaaatagtgg aaatgagaat
      661 gtcgcgattc ttgacgtgcg agagctcgtg tcccagcacg ccgcgcagtt cacgcggggt
      721 gagcaagcgc aagataccgg tggtagcggc caccgcagcg tgttcgggat tgcggccggt
      781 ggcaaaagca ttgggctggg cttcatcgat caaatacacc ttaggcttag gaagttctgc
      841 gcgttgtgca agctctcgca cgatgccgta gagtttcgga gcgctgcttt catccacttt
      901 ccgggcgtcg tacatcctga gcaccatttt gtcggagaac caatatgccc agagattcat
      961 agcgccaccg agaatcaggg caatgagtaa gcccgtgccg ccgccgagca gactgccgac
     1021 cacaccgaac agcgccatga tggccgccat cagtaaagag gtcttaaacc agtttcccaa
     1081 catcaacgtt ccttttgctt aaatgccact agtcgtggta catcgttaga tagtgtctgc
     1141 gagttaaaac ttcaagcgcc aaggcgtgcg ccttcccgta tggcaaaacc ggaaaggaag
     1201 gcttgcgctg gcaggcgctt gcctcccgct ttttgcagct cctcaatgcg caagccgccg
     1261 ctgccgcaag caaccacaat tccccggtca tcggcgctga tcacttcccc cggttttccg
     1321 tgtgcttgat aatccaggcg cgtcttccag attttcagcg tctctccgtt taacatggtg
     1381 taggctcccg gttggggatt gaaagcgcgc accgcacgct cgatttggct tgccgcgcct
     1441 tgccaattta tttgcgcttc ggttttctgc accttggcgg catagctcgc gccggtggcg
     1501 ggttgcggct gcggccgtaa ctgtccccgc tccaaggcac gcagggcgcg cacgatgctt
     1561 tcgccgccaa gcctggccag cttgtcatgc aagctttgcg cgttgtcgtc cgcggcaatg
     1621 ggtatccgtt cttgcatcaa aacgggtccc gtatccaagc cttcatccat ctgcatgatg
     1681 ctgacaccgg tttctgcgtc tccggccagc aaagcccgct gtattggcgc cgcgccgcgc
     1741 cagcggggca gaagcgaggc atgaatattg atgcagccca gccgcggtag gtccaacatc
     1801 gtcttcggta gtatcaaccc atacgcagcg accactatta catccgctac tgcccgttgc
     1861 aattccttca gcatttc
//
LOCUS       DATIOW010000186        10614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_794934, whole genome shotgun sequence.
ACCESSION   DATIOW010000186 DATIOW010000000
VERSION     DATIOW010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10614
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794934"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..290)
                     /locus_tag="VLL03_06200"
     tRNA            complement(214..290)
                     /locus_tag="VLL03_06200"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(254..256),aa:Asp,seq:gtc)
     gene            complement(319..394)
                     /locus_tag="VLL03_06205"
     tRNA            complement(319..394)
                     /locus_tag="VLL03_06205"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(359..361),aa:Val,seq:tac)
     gene            complement(478..750)
                     /locus_tag="VLL03_06210"
     CDS             complement(478..750)
                     /locus_tag="VLL03_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HSS46988.1"
                     /translation="MNKTEIIDHIARTADISKAAAGRAIDSTVAAIREALKKGRMVTL
                     AGFGTFYVGKRAARNGRNPRTGATIKIRAAKVPKFRAGKALKDDVN"
     gene            complement(953..3361)
                     /gene="lon"
                     /locus_tag="VLL03_06215"
     CDS             complement(953..3361)
                     /gene="lon"
                     /locus_tag="VLL03_06215"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HSS46989.1"
                     /translation="MSDQPVNEQQALYSLLPLRDVVVFPHMVIPLFVGRPKSIKALEA
                     AMESGKSILLVAQKSAAKDEPTPEDMYRVGSVANILQMLKLPDGTVKVLVEGSQRAVI
                     SEVIDAKTHYTAQPELLPADSAEGHEIEAMRRATLNQFDQYVKLNKKIPPEILTSLAG
                     IDSAGRLADTIAAHLPLKLEQKQQVLEMFDVKQRLEHLLSLLETELDILQVEKRIRGR
                     VKRQMEKSQREYYLNEQVKAIQKELGEMEEGADLDEVEKKIKAAHMPREARHKAEAEL
                     KKLKLMSPMSAEATVVRNYIDALISLPWKKRSKINKDLSNAVKVLDADHYGLEKVKER
                     IVEYLAVQQRVDKLKGPILCLVGAPGTGKTSVGQSIARATNRKFVRMSLGGVRDEAEI
                     RGHRRTYIGSMPGKILQNMAKVKVRNPLFLFDEVDKMGMDFRGDPSSALLEVLDPEQN
                     HSFVDHYVEVEYDLSDVMFVTTANTLNIPSPLLDRMEVIRLSGYTEDEKVHIAMRHLL
                     PKQMKNHGLKAEELALSESAVRDIVRYYSREAGVRALEREISKICRKVVKTMLLKKGK
                     ESKLAVTAKNLNKYLGVRRFTYGMAEKKNQVGQVTGLAWTEVGGELLTIEAVVLPGKG
                     KMITTGKLGEVMQESVQAALSVVRSRSRTLGIPEDFYQKNDVHIHLPEGATPKDGPSA
                     GIGVCTAMISILTGIPVRADVAMTGEITLRGEVLPIGGLKEKLLAAHRGGIKTVLIPE
                     ENVKDLAEIPENIKNKLDIHPVQWIDRVLELALERTPKPLPEEVTPPLPAVVEKTPAV
                     VIKH"
     gene            complement(3460..4728)
                     /gene="clpX"
                     /locus_tag="VLL03_06220"
     CDS             complement(3460..4728)
                     /gene="clpX"
                     /locus_tag="VLL03_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HSS46990.1"
                     /translation="MSDKIGGEKLLYCSFCGKSQHEVRKLIAGPSVFICDECIELCND
                     IIREEAHGEPGAKPGKSDLPVPREIRKILDQYVIGQDQAKKILSVAVYNHYKRLKTLS
                     KNTDDIELAKSNILLVGPTGSGKTLLAQTLARLLNVPFVMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYEVEKAQRGIVYIDEIDKISRKSENPSITRDVSGEGVQQALLKLIEGT
                     IASVPPQGGRKHPNQEFVQVDTTNILFVCGGAFDGLDKIIRNRTQNVGIGFGADVKSR
                     DNRKDIGEVLRDVEPEDLIKYGLIPEFVGRLPVVATLEELDEKALIQILTEPKNALLK
                     QYQKMFGMEGVELEVREPGLKAIAEKALARKTGARGLRSILELILLDTMFDLPSMENV
                     AKVVVDEGVINGDVKPLLMYSDQSKVAGSI"
     gene            complement(4765..5394)
                     /gene="clpP"
                     /locus_tag="VLL03_06225"
     CDS             complement(4765..5394)
                     /gene="clpP"
                     /locus_tag="VLL03_06225"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HSS46991.1"
                     /translation="MTQNRSREPQGLGLIPMVIEQSGRGERAYDIYSRLLKERVVFLV
                     GPVDEITANLIVAQFLFLESENPDKEIYFYINSPGGSVSAGLAIYDTMQFIKPDISTL
                     CVGQAASMGALLLAAGAKGKRFCLLNSRVMIHQPMGGFQGPASDVEIHAKEILYLRQR
                     LNEIMAKHTGQNIKTIEKDTDRDNFLSGEEAVKYGIADKVLVNRTETTA"
     gene            complement(5378..6697)
                     /gene="tig"
                     /locus_tag="VLL03_06230"
     CDS             complement(5378..6697)
                     /gene="tig"
                     /locus_tag="VLL03_06230"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HSS46992.1"
                     /translation="MQANVENLGQLQRRLNMMVPLDKIDNEVENRLKRLARSVKLHGF
                     RPGKVPFKIVVQQYGPQVLQEVLGDALQKSFSEAVREQNLRVAGYPKFEAKTAQAGGQ
                     QFEYTATFEVYPEVKVGEVNRAAITRPLVEIGDAQVEKTLEILRKQRAAYQTVGREAR
                     ERDQVTIDFHGKIGEEDFPGGQGTNLATVLGDKRLLPDFEKKIAGMRANETKTFELTF
                     PADYHGKEVAGKTAVFELVLKRVAEPKLPEVDAEFARSLGIADGDLEKLKREIRDNLQ
                     KEAGNRIQARIKDQAMQALLDGTQVDLPQSLVEMEVHRLMDNARRDLEARGVKTRDLQ
                     LPHDLFETQAKRRVALGLIISELAKLHDLHPKPEQVRARVDEHVQSYEHPEQVVKWYY
                     SEPGRLNDVEALVLEDNVVAWVMNRTKVAEKAMSFDELMGNANDAKS"
     gene            complement(6748..6829)
                     /locus_tag="VLL03_06235"
     tRNA            complement(6748..6829)
                     /locus_tag="VLL03_06235"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6793..6795),aa:Leu,seq:tag)
     gene            6986..7819
                     /locus_tag="VLL03_06240"
     CDS             6986..7819
                     /locus_tag="VLL03_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HSS46993.1"
                     /translation="MTPNEYCQDKAASSGSNFYYSVLFLPKGTRLALTALFACFREIQ
                     EIVQECTDPAVAKIKLQWWRQEITAAFSEQARHPITRALLPSTRQFGLSPDEFLKIID
                     GVETGLAQNRHADFASLKVYCCSTHGVAEQLAARILGVHDPDTLKCALELGLAFGLTD
                     VILNVRDDARRNRILLPQDELARFGVTAADLLNLRETEGFKKMIEFAIDSTENCYNRA
                     ISEFPAAERKAQRGRLALAAINRALLREIRKDGCRILRRRVDLTPIRKLWLAWIAWME
                     G"
     gene            7873..8250
                     /gene="speD"
                     /locus_tag="VLL03_06245"
     CDS             7873..8250
                     /gene="speD"
                     /locus_tag="VLL03_06245"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HSS46994.1"
                     /translation="MTSMIISTLSGLHLIGDLYQCDPTHLSDSTWLRKRCTQLCKNAG
                     LTVLGDFFHQFGDAGFTGTLVLAESHIAIHTWPETSTVALDVFVCNYSRDNSDKAQRL
                     FDDVLKLLKPKEVIRQAVQRGRL"
     gene            8247..9098
                     /gene="speE"
                     /locus_tag="VLL03_06250"
     CDS             8247..9098
                     /gene="speE"
                     /locus_tag="VLL03_06250"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HSS46995.1"
                     /translation="MSEDKLLTEWLNTNCGFFYRAGKQLEEYDTPYQHLEVYDTPEVG
                     RLFRLDGCFMTSEKEEFFYHENLIHPAAIAHPAPRKALIIGGGDGGSSEELLKHAGIE
                     SVILVELDQAVVDISKKYFHAVHQNVFDDPRLKLHIADGMTFVRETRERFDLIALDLP
                     DPIGPATELYTAEFFASCRRVLNPGGALSLHVGSPISRPDRVRDNIVNLKKVFRIVRP
                     YLVYIPLYGSLWGMACVSDSLDPQTLAPEEVERVLKDRRIADLRYYNGHTHQAVFALP
                     NFVRRIT"
     gene            complement(9095..9226)
                     /locus_tag="VLL03_06255"
     CDS             complement(9095..9226)
                     /locus_tag="VLL03_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS46996.1"
                     /translation="MSCTGDKNYAVFEQVGRRLIVSHGLDEKVRWLKAQETGLVPDS"
     gene            9185..9353
                     /locus_tag="VLL03_06260"
                     /pseudo
     CDS             9185..9353
                     /locus_tag="VLL03_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009387635.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            complement(9571..>10614)
                     /locus_tag="VLL03_06265"
     CDS             complement(9571..>10614)
                     /locus_tag="VLL03_06265"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HSS46997.1"
                     /translation="ATIDDVIVGCAMPEGEQGMNVARIALLLAGFPNRVSGMTINRFC
                     SSGLQAVALAADRIRLGEAEVMIGAGTESMSMVPMGGNKIAANPRIFNDENIGIAYGM
                     GITAEKVASRWKIGREAQDQFALQSHQRAIRATESGEFKQEITPYPVAETIPDLESRE
                     VVEKTREVSVDEGPRRDTSLEALGKLKPAFAAKGSVTAGNSSQMSDGAAAVILMSEAA
                     LKRFNLKPLGRFLGYAVAGVPPEIMGIGPKEAIPKVLKQVGLKQDDLDWIELNEAFAA
                     QSLAVIQDLGLDRTKVNPMGGAIALGHPLGATGAIRTATLTHGLRRHNKKYGMVTMCI
                     GTGMGAAGLFEAL"
BASE COUNT         2122 a         3210 c         2833 g         2449 t
ORIGIN      
        1 ccacgtaatc tccgccgccg atgctgctga aatagcgttc cacgccgaac agcgcaaacg
       61 gcaggaagat caaagccagg acgatctggg cgactctctt gtgattccga aaaaaatcaa
      121 acatgttttg agataagttc cggtccggga tataaaaaag gcgaaccggt gttcgcctct
      181 aaggattctc tgcagaactt cacttcgaat ctgtggcgga gtggacggga ctcgaacccg
      241 cgacccccgg cgtgacaggc cggtattcta accaactgaa ctaccactcc tctaatatta
      301 aacctgcctg aattccgctg gtgggtgctg agagggtcga actcccgaca ttcgccttgt
      361 aagggcgacg ctctaccaac tgagctaagc accctaaacc cggctgaggt gttgccattg
      421 cctaagcgcg agcctttttc agccagggct caagccgacg ctctgattga aaaggcgcta
      481 gtttacgtca tccttcagcg ctttgccagc gcggaatttc ggaactttgg ccgctctgat
      541 tttgatggtg gcgccagtgc gcgggttgcg gccgttgcgg gcggcgcgct tgcccacata
      601 aaaagtgcca aagccggcta gggtgaccat tctccctttt ttcaatgcct ccctgatcgc
      661 cgcgaccgtg ctgtcgatgg cgcggccggc agcggccttg gaaatgtccg ctgtcctggc
      721 gatatgatca atgatttcag ttttgttcac gaaaaatccc ccttcgttgg ataagctatc
      781 agaaattgat gaatgaatta gtgcgggaac taaacttaaa accctatact cgctttatag
      841 caagcgaaaa aagcgcgtgt caagcaagta aagcgcaaat ggaaaataaa aaagaaacgc
      901 gactggaatc cgtgcgccgg acgacgagaa atgtcgtctg ccgacgacag attcagtgct
      961 tgatcactac agcgggggtt ttttccacca ctgccggcag cggcggcgtc acctcttcag
     1021 gcagcggctt gggtgtgcgt tccaaggcaa gttccagaac ccggtcgatc cattgcaccg
     1081 ggtggatatc gagcttgttc ttgatattct ccggaatttc agccaagtcc ttgacgttct
     1141 cttccggtat caatacggtc ttgatgccgc cgcgatgtgc cgcaaggagt ttctccttaa
     1201 ggccgccgat cggcaacacc tcgccgcgca aggtgatttc gccggtcatc gccacatcgg
     1261 cgcgaaccgg aatcccagtc aatatcgaaa tcatcgcggt gcaaacaccg atgccagcgc
     1321 tcggcccgtc cttgggcgtc gccccttcgg gcaaatggat atgcacgtcg tttttctgat
     1381 agaaatcctc gggaatgccg agcgtgcgcg aacggctgcg caccaccgac agcgccgcct
     1441 gcaccgattc ctgcatcact tcgccgagct tgccagtggt aatcatcttg cccttgccgg
     1501 gcagcaccac cgcttcaatg gtcaataact ctcccccgac ttcggtccag gccagcccgg
     1561 tgacctggcc cacctgattt ttcttttcgg ccatgccgta ggtgaagcgc cgcactccaa
     1621 ggtatttgtt gaggtttttc gcggtcaccg cgagcttgct ctccttaccc ttcttgagca
     1681 gcatggtttt caccactttg cggcagattt tcgagatttc gcgctccagc gcgcgcaccc
     1741 cggcttcacg gctgtagtag cggacgatgt cgcgcaccgc gctttctgaa agcgcaagct
     1801 cttccgcctt cagtccatga tttttcatct gcttcggcag cagatggcgc atcgctatat
     1861 ggactttttc gtcctcggtg tagccggaaa gccgtatcac ttccatgcga tcaaggagcg
     1921 gtgatggaat gttcaaggtg ttcgcggtcg tcacgaacat cacatcggag aggtcgtatt
     1981 ccacttcgac atagtgatcg acaaacgaat ggttctgctc gggatccagc acttccaaga
     2041 gcgccgaaga cggatcgccg cggaaatcca ttcccatctt gtccacttca tcgaacagga
     2101 acagcgggtt gcgcaccttc accttggcca tgttttgcag gattttaccc ggcatggatc
     2161 cgatgtaagt gcggcggtgg ccgcggattt cggcttcgtc acgcacgccg cccagcgaca
     2221 tacgcacaaa cttgcggttg gtggcgcggg caatggattg accgaccgag gttttacccg
     2281 ttcctggtgc ccctacaagg cacaagatcg gccctttcag cttgtccacg cgttgctgca
     2341 ccgccaggta ttccacgatg cgctccttga ccttttccag cccgtagtga tcggcgtcga
     2401 gcaccttgac cgcgttcgag aggtccttat tgatcttgct tctttttttc cacggcagcg
     2461 atatcagggc gtcaatgtag ttgcgcacca cggtggcttc cgccgacatc ggcgacatca
     2521 gcttgagctt cttaagctcc gcttcggctt tatgacgcgc ctccctgggc atatgcgcgg
     2581 ccttgatttt tttctcgact tcatccagat cggcgccttc ttccatctcg ccgagttcct
     2641 tctgaatggc tttcacctgc tcgttcagat aatactcgcg ctggcttttc tccatctggc
     2701 gcttgacgcg gccgcgaatg cgcttttcca cttgcaaaat gtcgagttcg gtctcgagca
     2761 ggctcaacag gtgttcgagc cgctgtttca cgtcgaacat ctccagcacc tgctgctttt
     2821 gctccagttt caacggcaga tgcgcggcga tggtatccgc caggcgccca gcactgtcga
     2881 ttccggcaag cgaagtgagg atttccggcg ggattttctt gttgagcttg acgtattggt
     2941 caaactgatt gagcgtggcg cgccgcatcg cctcgatttc gtggccttca gcgctgtcgg
     3001 cgggcaggag ctccggctgc gccgtgtaat gggtcttggc gtcaatcacc tcgctgataa
     3061 cggctcgctg gctgccttcg accagcactt tcaccgtgcc gtcggggagt ttcagcattt
     3121 gcaggatgtt ggcgacactg ccgacccggt acatatcctc cggggtaggc tcatccttgg
     3181 cggcggattt ctgcgccacc agcagaatgc tcttgccgct ttccattgcc gcttccagcg
     3241 ccttgatgga tttcggacgt cccacgaaca gcgggatgac catgtgcggg aacaccacca
     3301 cgtcgcgcag cggcagcagg gagtacaggg cttgttgttc gttaaccggt tggtcagaca
     3361 tgtcgttgat acccctgata gaagataaaa tcgatatgtg ggcggcaccc ttaaaaatca
     3421 agaagcataa gccatgccat ccgccaggcg gggcttaagt tagattgagc ccgcgacctt
     3481 cgactggtcg gaatacatca gcaacggctt gacatcgccg ttaatcacgc cctcatcgac
     3541 caccaccttg gccacgttct ccatcgaggg caggtcaaac atagtgtcga gcaagatcag
     3601 ttcgagtatg gagcgcaaac cccgagcacc ggtcttgcgt gccaatgctt tttcggcaat
     3661 cgctttcaag cccggttcac gcacctccag ttccacgccc tccatgccga acattttctg
     3721 atactgcttg agcagcgcgt tcttcggctc ggtcagaatc tgaatcaacg ccttctcatc
     3781 aagctcttcc agggtggcca ccaccggcag gcggccgacg aattcaggaa tcaggccgta
     3841 tttaatcagg tcttccggct cgacatcgcg cagcacttcg ccgatgtcct tgcggttgtc
     3901 gcggcttttg acatccgcgc cgaagccgat gccgacattc tgcgtgcggt tgcggatgat
     3961 tttgtccagc ccgtcaaacg cgccgccgca gacgaacagg atattggtcg tgtcgacctg
     4021 cacgaactcc tgattgggat gcttgcgccc gccctgcggc ggcaccgagg caatagtacc
     4081 ctcgatgagt ttcaacaatg cctgctgcac gccctcgccg gaaacgtcgc gggtgatcga
     4141 ggggttttcg ctcttgcgcg aaattttgtc aatttcatct atataaacga tgccgcgctg
     4201 cgctttctcg acttcgtagt cgcatttctg cagaagcttc tggatgatgt tttccacgtc
     4261 ttcgccgacg tagcccgctt cagtcagcgt ggtcgcatcc gccatcacaa aaggcacgtt
     4321 tagcaaccgc gcgagcgttt gcgccagcag cgttttgccc gaaccggtgg gaccgaccag
     4381 gaggatattg ctcttggcga gctcgatgtc gtcggtgttc ttgctgaggg tcttcagccg
     4441 tttatagtgg ttatagaccg cgaccgacag aatctttttc gcctggtcct ggccgatgac
     4501 atattggtcg aggatcttgc ggatttctcg cggcaccggc agatcggatt tgcccggctt
     4561 ggcgcccggc tcgccgtgcg cttcctcgcg gatgatgtcg ttgcacagct cgatgcactc
     4621 atcgcagatg aaaaccgatg gcccggcaat gagcttcctc acctcatgct ggctcttgcc
     4681 gcagaaggag cagtaaagga gcttctcccc gccgatttta tctgacatga ctgatttcct
     4741 cctcgcctct acttacgtgg gacatcaagc agtggtttca gttctgttca ccaacacttt
     4801 gtcggcgatg ccgtacttga ccgcctcctc gccgctcagg aaattgtcgc ggtcggtatc
     4861 tttctcaatc gtcttgatgt tctgtccggt gtgcttggcc atgatttcat tgagccgctg
     4921 ccttaaataa agaatttcct tggcgtggat ttccacgtcg gaggccggtc cctggaaacc
     4981 gcccatcggc tgatgaatca tcacccgcga attgaggaga cagaagcgtt tgcccttggc
     5041 gcccgccgcg agcaaaagcg cgcccatgct cgccgcctga ccgacgcaca gcgtgcttat
     5101 atccggtttg atgaactgca tggtgtcgta aatggcgagc cctgccgaca ctgagccccc
     5161 gggcgaattg atgtagaagt aaatctcctt gtccgggttt tcggattcga ggaataaaaa
     5221 ttgcgccacg atcaggttgg cggtgatttc atccaccggc ccgaccagga acaccacgcg
     5281 ctccttgagc aggcgcgagt aaatatcgta cgcgcgctcg ccgcgcccgc tctgctcgat
     5341 caccatcggg ataagaccga ggccctgcgg ttcccgacta cgattttgcg tcattggcat
     5401 ttcccatcag ttcatcaaac gacatcgctt tctctgcaac cttggtccgg ttcatcaccc
     5461 aggccaccac attgtcttcg agcaccagcg cttcgacgtc attcaggcgt cctggttcgg
     5521 aataatacca cttcaccacc tgctccgggt gctcgtagct ttgcacgtgc tcatcaaccc
     5581 gagcgcgcac ttgctccggc ttgggatgaa gatcgtgaag tttggcaagc tcggagataa
     5641 tcagcccgag cgcgacgcgg cgcttggcct gcgtttcgaa aagatcgtgt ggcagctgca
     5701 aatcccgggt tttgaccccg cgcgcttcga ggtcgcggcg cgcattgtcc atcaggcgat
     5761 ggacttccat ttccaccagc gattggggaa gatcgacctg tgtgccatca agcaaggcct
     5821 gcatcgcctg gtccttgatt ctggcctgta tgcggtttcc ggcttccttc tgcaaattgt
     5881 ccctgatttc gcgtttcagt ttctccaaat cgccatcagc aatgccgagg ctcctggcga
     5941 attcggcgtc cacctcgggc aatttcggtt ccgccacccg cttcaacacg agttcgaaaa
     6001 ccgcggtttt ccccgccact tccttgccat gataatccgc ggggaaagtt aattcaaatg
     6061 tcttggtttc gttcgcccgc atgccggcaa ttttcttctc gaaatccggc agcagccgct
     6121 tatcgcccag caccgtggcc aaattcgtgc cttgaccgcc gggaaaatct tcctcaccga
     6181 tcttgccgtg gaaatcgatg gtcacctggt cccgctcgcg cgcttcgcgt cccaccgttt
     6241 ggtaggcggc gcgctgtttg cgcagaattt ccagggtctt ttccacttgc gcatcgccga
     6301 tttccaccag tggccgggta atggcggccc ggtttacctc gccgactttc acttcgggat
     6361 aaacttcaaa cgtcgcggtg tactcgaact gttggccccc cgcctgcgct gtcttggctt
     6421 cgaacttggg atagcccgcg acgcgcaggt tctgctcgcg caccgcttcg ctgaagcttt
     6481 tttgcagggc atcgccgagc acttcctgga gaacttgcgg gccgtattgc tggacgacga
     6541 ttttgaacgg caccttgccc gggcggaagc cgtgcaattt gacgctacgt gcaaggcgct
     6601 tcaggcggtt ctccacttcg ttgtcaattt tatcgagtgg caccatcatg ttgaggcgcc
     6661 gttgcagctg acccagattt tccacattcg cctgcatttc gtttccttaa tagtcacctc
     6721 aagaataaaa gaagactttt cgccatgtgc gaaaggaggg actcgaaccc ccaagccttg
     6781 cggcgctgga acctaaatcc agtgcgtctg ccaattccgc cactttcgcg ctagacgtaa
     6841 gtttaatata ttaagtctgc tacggccagc tttaacttta agtatacacg ctaattcatt
     6901 gataaattat acaattgact agcgattgcg atctctggtg cacgcttgag tttcattctc
     6961 cgcaatgctt gaagcgggca ccgcaatgac accgaacgaa tactgccagg acaaggcggc
     7021 atcaagcggc tccaatttct actacagcgt gctgttcctc ccgaaaggca cgcgcctggc
     7081 gctcaccgcc ctcttcgcct gttttcggga aatccaggaa atcgtccagg aatgcaccga
     7141 cccggcggtg gcaaaaatta aattgcaatg gtggcgccag gaaatcaccg ccgccttctc
     7201 cgaacaagct cgacacccca tcacccgagc tttgcttcca tccaccaggc aattcggctt
     7261 gtcgccagat gagttcctaa aaatcattga cggcgttgaa acaggcctgg ctcaaaaccg
     7321 tcatgccgat ttcgcttcac tgaaagtata ttgctgctcc acccatggcg tagccgagca
     7381 gctggccgcg cgcatactcg gtgttcacga ccccgacaca ttaaaatgcg ccctagagct
     7441 gggactggct tttggtctta ccgatgtcat tctgaatgtg cgggacgacg cgcggcgcaa
     7501 tcgcattctg ctgccgcagg acgaactcgc gcgtttcggc gtgacggcgg cggatctgct
     7561 caatttgcgc gaaaccgagg gcttcaaaaa aatgattgaa tttgctattg acagcacaga
     7621 aaattgttat aatcgcgcaa tttctgaatt tccggcggcg gagcggaaag cacagcgcgg
     7681 aagactcgcg ctggcggcaa tcaaccgggc gctgcttcgg gaaatcagga aggacgggtg
     7741 ccgtatcttg cgacggcgtg ttgatctgac gcctatccgc aagctgtggt tggcgtggat
     7801 agcttggatg gagggatagc gcagattggg aggtttttcg gctgatagcc ttttggtgaa
     7861 gggggcggaa aaatgaccag catgatcatc agcacgttaa gtgggctcca tctgatagga
     7921 gatctttatc agtgtgatcc aacccatctt tccgattcaa cctggctgcg aaagcgctgt
     7981 acccagctct gtaagaacgc cggccttacc gtgctggggg atttcttcca ccagttcggc
     8041 gatgccggct tcaccggcac cttggtcctc gccgaatcgc acatcgccat tcacacctgg
     8101 ccggaaacct ccacggtggc gctcgacgtc tttgtctgca actacagccg tgacaacagc
     8161 gacaaggcgc aacggctttt cgacgacgta ctaaagctac tcaaacccaa ggaagtcatt
     8221 cgccaggccg tgcagcgcgg acggctatga gcgaggacaa gctgctcacc gagtggctga
     8281 acaccaactg cggctttttc taccgcgctg gcaagcagct cgaagagtac gacaccccct
     8341 accagcacct cgaggtctac gacacccctg aagtcggccg cctgttccgc ctcgacggct
     8401 gcttcatgac ctcggaaaaa gaagagttct tctatcacga gaacctcatc caccccgccg
     8461 cgatcgccca ccccgccccg cgcaaggcgc tgattatcgg cggcggcgac ggcggctcta
     8521 gcgaagagct gctcaagcat gccggcatcg aaagcgtcat cctggtcgag ctggatcaag
     8581 cggtcgtgga tatctcaaaa aaatatttcc atgccgtgca ccagaacgtc ttcgacgatc
     8641 cgcggctgaa actccacatt gccgacggca tgactttcgt ccgtgaaacc cgcgagcgct
     8701 tcgacctgat tgcgctcgac ctgcccgacc ccatcggccc cgccaccgag ctttacactg
     8761 cggagttttt cgcaagttgc cggcgcgtgc ttaatcccgg cggcgcgttg agcctgcatg
     8821 tcggctcgcc gatttcgcgc cccgaccgcg tgcgcgacaa catcgtcaac ctgaaaaaag
     8881 tgttcaggat cgtacggcct tacctggtct acatcccgct ctacggctcg ctgtggggca
     8941 tggcctgcgt ttccgacagc ctcgacccgc agacgctcgc gcctgaagag gtcgagcgcg
     9001 tgctcaaaga ccgccgtatc gccgatctgc gctactacaa cgggcatacc caccaggcgg
     9061 tattcgccct ccccaacttc gtgcgccgga tcacctagct atcgggcacc aagcctgtct
     9121 cctgcgcctt gagccagcgc actttttcat ccagaccatg cgagactatc aagcgccggc
     9181 cgacttgctc aaaaaccgcg taattcttgt caccggtgca ggacaaggct taggcggagc
     9241 cgcgggcact tgcttttgcc aaacatggcg cgacagtgat tctgcatggg cgcaaggtca
     9301 acaagcttga agctacctac gatgaaatcg agaaagcggg ctatccgcgg ccggtggcct
     9361 cgccccaacg cgccaaaacc tgtcccggcg aaatcaagga aaaactgccc aagcccgaaa
     9421 acctgatgcc gctgtatctc tatctgatgg atccggcagg caaaggcgtc tcgggccgaa
     9481 taattgattg tcggggttaa gaaacctctg attaagtccc gccgtcctga aatcgcatgg
     9541 tcaattgagc aaattaaaaa gtgattgggc ctacagcgcc tcgaacaagc cggcggcgcc
     9601 catgccggtg cctatgcaca tagtgaccat gccgtacttc ttattatggc ggcgcaatcc
     9661 gtgcgtcaag gtcgcggtgc ggatagcgcc ggtcgcgccc agcggatgcc cgagcgcgat
     9721 cgccccgccc atgggattga ctttggtgcg gtcgagaccc aagtcctgaa tcaccgcaag
     9781 gctttgtgcg gcgaaggctt cgttgagctc aatccagtcc agatcgtcct gctttaaacc
     9841 gacctgcttt aataccttgg gaatcgcttc cttggggccg atgcccatga tttccggcgg
     9901 caccccggct acggcatagc cgaggaagcg gccgagaggt ttgagattga aacgcttgag
     9961 cgccgcttca gacattaata tcaccgccgc agcgccatcg gacatttgcg agctgttgcc
    10021 cgccgtcacc gagcctttcg ccgcaaaagc gggtttaagt ttccccagcg cttcgagcga
    10081 ggtgtcccgg cgcggcccct cgtccaccga cacctctctg gtcttttcca ccacttccct
    10141 gctctccagg tcgggaatgg tttcggcgac cgggtaaggc gtaatttcct gcttgaattc
    10201 gccgctctcg gtcgccctga ttgcgcgctg atgactttgc agggcaaact ggtcctgtgc
    10261 ttcacgaccg attttccagc ggctcgccac tttttccgca gtgatgccca tgccgtaagc
    10321 gatgccgata ttttcatcgt tgaaaatgcg ggggtttgcc gcgattttgt tgccgcccat
    10381 cggcaccatg ctcatgcttt cagtccccgc gcctatcatc acttccgctt cgcccaaacg
    10441 gatgcgatcg gcggctagcg ccaccgcctg caggccggaa gagcagaaac gattaatggt
    10501 cattcccgaa acgcggttgg ggaaaccagc gagcaacaac gcgatgcgcg ccacgttcat
    10561 cccctgctcg ccttccggca tggcgcagcc cacgatcaca tcgtcgatgg tggc
//
LOCUS       DATIOW010000187         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_285659, whole genome shotgun sequence.
ACCESSION   DATIOW010000187 DATIOW010000000
VERSION     DATIOW010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285659"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VLL03_06270"
     CDS             <1..567
                     /locus_tag="VLL03_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HSS46998.1"
                     /translation="PLNVVGIIPTTENMPGGNASRPGDIVTSMSGQTIEILNTDAEGR
                     LILCDALTYAERFHPALVIDIATLTGACVIALGHVASGLFSSDEALARELLHAGDYAH
                     DRAWQMPLWDDYQDQLKSNFADMANIGGRPAGSITAACFLARFTKKYSWAHLDIAGTA
                     WKSGREKGATGRPVPLLAQFLMHKAAKA"
     gene            573..1007
                     /locus_tag="VLL03_06275"
     CDS             573..1007
                     /locus_tag="VLL03_06275"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HSS46999.1"
                     /translation="MTQIDFFSHADNKLQVACSLSAQALERGLRVLIYTPDADTTDKL
                     DKLMWCYPAIGFIPHCRAADRLAAETPVIIDHLAEPLPHDEVLLNLHPELPPFFSRFQ
                     RLIEIVGADETDNRAGRVRFRFYRDRGYEIRHHDLNQSPGSL"
     gene            1017..1442
                     /locus_tag="VLL03_06280"
     CDS             1017..1442
                     /locus_tag="VLL03_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS47000.1"
                     /translation="MAKQDDILDKMDALMKKHGALGGDEAIPTLTEVIAGGVKRAGLP
                     ALSAEQVETLAIEIYQHVLQNLDARINNELQQHLRPQLARMLEQVLERMLAELKNSIK
                     SMVDDAVAHALDAQLEHHQKISQSSTPAAKRSGNTGTPD"
     gene            1745..>2407
                     /locus_tag="VLL03_06285"
     CDS             1745..>2407
                     /locus_tag="VLL03_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HSS47001.1"
                     /translation="MELPKSFDPHGIESRWYDEWEKRGYFAHSTDSSKPSYSIQLPPP
                     NITGTLHMGHAFQQTLMDILIRYHRMRGFNTNWVIGTDHAGIATQIVVERQLEMENKT
                     RRELGREKFIERVWQWKQQSGSTITRQMRRLGVSGNWDYASTDGRHAGYFTMDERMSR
                     AVIEVFVQLYEQGLIYRGKRLVNWDPVLGTAVSDLEVDSEEENGKLWEIHYPLESGGK
                     GVV"
BASE COUNT          536 a          722 c          684 g          465 t
ORIGIN      
        1 ccgcttaacg tggtgggcat cattcccacc accgaaaaca tgcccggcgg caatgccagt
       61 cggccgggcg atattgtcac cagcatgtca gggcaaacca tcgaaattct caataccgac
      121 gccgaaggcc gcctcattct gtgtgatgcg ctcacctacg ccgaacgttt ccacccggcc
      181 ttggtaattg acatcgccac actcaccggc gcttgcgtga ttgccctcgg tcatgtcgcg
      241 tccggtcttt tcagcagtga cgaagctctg gcgcgcgagt tgctgcacgc gggcgattac
      301 gcacatgacc gtgcctggca gatgccgctg tgggacgatt atcaggacca gttgaaaagc
      361 aatttcgccg acatggcgaa catcggcggc cgccctgcag gcagcatcac tgcggcttgt
      421 ttcttggcgc gcttcaccaa gaaatactcc tgggcgcacc ttgatatcgc gggcacggcg
      481 tggaagagcg gcagggaaaa aggcgctacc ggacggccgg taccattgct cgcgcaattt
      541 ttgatgcaca aagcagctaa agcataacgc cgatgacaca gattgatttc ttctctcatg
      601 ccgataacaa gctgcaagtt gcctgcagct tgagcgctca agcgctggaa cgcggcctgc
      661 gcgtattgat ttacaccccc gacgccgaca ccacggataa gctcgacaag ctgatgtggt
      721 gctaccctgc aatcggcttc atcccacatt gccgcgccgc tgaccggctg gcggcggaaa
      781 caccggtgat tattgaccat cttgcggaac cgcttcctca cgacgaagtg ctgcttaatc
      841 tgcatcccga gctcccgccg tttttcagcc gctttcagcg cctgatcgaa attgtgggcg
      901 cggatgaaac tgacaaccgg gcgggacgcg tgcgtttccg tttctaccgc gaccggggat
      961 acgagatacg ccatcacgat ctcaaccaat ccccgggttc tttataaaaa ggttctatgg
     1021 caaaacagga cgacatcctg gataaaatgg atgcgctgat gaaaaaacac ggcgcgctgg
     1081 ggggtgacga agccataccc acgctcaccg aagtcattgc cggcggggtt aagcgcgcgg
     1141 gattgcccgc actgagcgca gagcaggtgg aaacgctcgc aatcgaaatc tatcaacacg
     1201 tcctgcaaaa tctcgatgct cgcatcaaca acgaactgca gcagcacctc aggccgcaac
     1261 ttgcgaggat gctggagcag gtgctggagc ggatgctcgc cgagctcaag aacagcatca
     1321 agagcatggt tgacgacgca gtggcccatg cgcttgatgc gcagctcgaa caccatcaga
     1381 aaattagtca atcctctact cccgctgcaa agcgctctgg caacacggga acccctgatt
     1441 aagtcccgca cgtgcacgac ggtgtgcaat ccgggcgcag ggcaaggcgt gaggagcgcc
     1501 gtttggtaga ggccaaacaa gcgacgaaca acaaagccat acggccggat tgccccgtcc
     1561 catccgggtt gcggcgattg agcggccatc tgcggtgttg ctcgggcttg cgttatgtcc
     1621 tgatagcgct gcgccctcgc gcctagcatc tggccgcccc tcgtccgcaa cgcgctacgc
     1681 gtggggctca atcaggggtt cctcaggtat aatcgctgct tttcgttcct ccgtttcacc
     1741 cgatatggaa cttcccaaga gttttgatcc gcacggcata gagagccgct ggtatgacga
     1801 atgggaaaag cgcggctatt tcgcccattc gacagattct tccaagccct cttattccat
     1861 tcagctccct ccgcctaaca tcaccggcac cctgcacatg gggcatgcgt tccagcagac
     1921 gctcatggac atcctcatcc gctaccaccg catgcgcggc ttcaacacca actgggtgat
     1981 aggcaccgat catgccggca tcgccacgca aatcgtggtc gagcgccagc ttgaaatgga
     2041 aaacaagacc cgccgcgaat tgggccggga aaaattcatc gagcgcgtct ggcaatggaa
     2101 gcagcaatcg ggatcgacca tcacgcgcca gatgcgccgc ctgggcgtct ccggcaactg
     2161 ggattacgcg agcactgacg gccggcatgc gggttacttc acgatggacg agcgcatgtc
     2221 gcgcgcggtc attgaagtgt ttgtgcagct ctacgaacaa gggctgatct atcgcggcaa
     2281 gcggttggta aattgggatc cggtgctggg caccgcggtg tccgacctcg aagtcgacag
     2341 cgaggaagag aacggcaagc tttgggaaat ccactatccg cttgaaagcg gcggcaaggg
     2401 agtggtg
//
LOCUS       DATIOW010000188         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_896623, whole genome shotgun sequence.
ACCESSION   DATIOW010000188 DATIOW010000000
VERSION     DATIOW010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896623"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1070)
                     /locus_tag="VLL03_06290"
     CDS             complement(141..1070)
                     /locus_tag="VLL03_06290"
                     /inference="COORDINATES: protein motif:HMM:NF006452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HSS47002.1"
                     /translation="MDMNVPHAVLQIPKNYSVRQPVTESAELSFSPQLRVYFDKEHRA
                     MWSRWNPEPRPCFNPRLLDGIKCYYDYLAQRGGAIQCGPEESPVEYVVLASGTPGVFN
                     LGGDLDLFKQLIETRDREGLLNYGRACVEVLYRNYMGHDLHATTISLVQGECLGGGFE
                     AALSSDVIIAEKKARFGFPEILFNLFPGMGAYSFLKRRVGQKVTEELITSGKIYSADD
                     MLAFGVVDVVAEDGQGEAEVAEFIKRRQKTHNGFTALSQVRRRVDQIDLSELLDVVAL
                     WVDCALRLTLRDLKLMQRLVSRQNGIGDSQQIH"
     gene            1394..>2591
                     /locus_tag="VLL03_06295"
     CDS             1394..>2591
                     /locus_tag="VLL03_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSS47003.1"
                     /translation="MQNPTLPSGSPQTLFEAFLDAVKTHGRTRRVLEDMKGEQSYGQL
                     LKTSLALGRFACRVSREQETVGVLLPNLIVTVCLFLGLSAMRRIPAMLNYSSGIEAIR
                     GACTAANIRTVITSREFLEAAKLHAVVPSLNDVKLVYLEDVRKSFTLADKLWLLRALW
                     RPRLAMYASDPNEPAVVLYTSGSEGKPKGVALSHKAILSNIAQIRAAVDVVPSDKFLN
                     ALPIYHSYGLTACALLPLLGGARVFIYISPLHYRTIPEIAGSRKCTFLFGTSTFLGEY
                     AKVASPKDFQFLRYVISGAEKLSEEVARTWLEKFGLRIMEGYGATECAPVLAVNSNLA
                     YRRGAVGRFLQGIEFRLQKIPGIGHGSLLHVRGPNVMLGYYLQERPGELQSTHSEFGP
                     GWYNTGD"
BASE COUNT          537 a          750 c          718 g          586 t
ORIGIN      
        1 tcccagcggc gcagcttctg aatcgggatg cccagcttgg ctgagacttc ctgaatagtg
       61 tagtcgttcc ccggcattat gcttcctatg cccataatcg gctgcccgcg ggaatgcgtt
      121 ccccgctggc acgacggcgc ttagtggatc tgctgcgaat cgcctatgcc gttttgccgc
      181 gacaccaggc gctgcatcaa ctttagatca cgcagggtta gccgcaatgc gcagtccacc
      241 cacagcgcga cgacgtccag caattcggat aagtcgatct ggtccacccg ccgccgtact
      301 tgggacaagg cggtgaaacc attatgggtt ttctgccggc gcttgatgaa ctccgcgacc
      361 tcggcttcgc cttggccgtc ttctgcaacc acgtccacca cgccaaacgc cagcatatca
      421 tcagcgctat agattttgcc actggtaatg agttcctcgg ttactttctg gccaacgcgc
      481 cgcttgagga aggaataggc ccccatgccg gggaacaggt tgaacaggat ttccgggaag
      541 ccgaagcgcg cttttttctc ggcgatgatc acgtcgctgg aaagcgccgc ttcaaagccg
      601 ccccctaggc attcgccctg caccagcgaa atggtagtgg cgtgtagatc atggcccatg
      661 tagttgcggt aaagcacttc gacgcaggcg cgtccgtaat tcagcaggcc ctcgcggtcg
      721 cgtgtctcga tcagttgctt gaataaatcg aggtcgccgc ccagattgaa aacgccgggc
      781 gtgccggagg cgagcaccac gtattccacc ggggattctt caggcccgca ctggatggcc
      841 ccgccgcgct gcgccagata gtcgtaataa cacttgattc catccagcaa gcgcgggttg
      901 aagcaaggtc gcggttcagg gttccagcgc gaccacatgg cgcgatgctc cttatcaaag
      961 taaacgcgaa gctgcggtga aaaggacaac tccgcgcttt cggtgaccgg ctggcggacg
     1021 gaataattct taggaatttg caacacagcg tgggggacat tcatatccat ggttttctcc
     1081 ggtaaaagtg atttagaatg cgggggtttt gttacccccc tctgtttctc gatcgttttt
     1141 tcgaaccgtg aaattgaatt taagaatata ttacgcgaaa aagcaaatct aacaaattga
     1201 tagattttgc ttttttgtca cggctcggac attttgccat ccccgactgt cagtccaggt
     1261 ttcgctaccc ttatttgtta taaaatactt aaaacttctg ggcttaaaca ttgattttcc
     1321 tccgccttct ttgccgtgtt ctgttccgga ctctgacagg tgcgcttggg ctatgcgctc
     1381 aaaagctgtc ctgatgcaaa atccaacgct cccttccggc tcgccgcaaa ccctgttcga
     1441 ggcctttctt gacgcggtta aaacgcatgg ccgtacccgc cgggtgctcg aagacatgaa
     1501 aggggagcaa tcctatggac aactgctcaa gaccagcctt gcgctcggca ggtttgcctg
     1561 ccgcgtgagc agggagcagg aaaccgtcgg ggtgctgctg cccaatctca tcgtcaccgt
     1621 ttgcctgttt ctgggcttaa gcgccatgcg caggattccg gccatgctca actacagttc
     1681 ggggatcgag gcaatccgcg gcgcgtgcac ggccgccaat atccggaccg tcatcacctc
     1741 gcgagaattt ctggaagcgg ccaaactgca cgccgtagtg cccagcttga acgatgtgaa
     1801 gctggtttat ctggaagatg taagaaaatc atttaccctt gccgacaaac tgtggctgct
     1861 gcgagcgctg tggcggccgc gcctggcgat gtacgcgagc gatcctaatg agccggcggt
     1921 cgtgctttat acctcgggct cggaaggcaa acctaagggc gtggcattaa gccacaaagc
     1981 gatactctcc aatatcgcgc aaatcagggc ggcggtcgat gtcgtgccct ccgacaaatt
     2041 tctcaatgcg ctgccgattt accattccta cgggcttacc gcctgcgcgc tgctgcctct
     2101 gctaggaggc gcgcgggtgt tcatctacat ttcgcctttg cactatcgca ccatcccgga
     2161 aatcgccggc agccgcaaat gcactttttt gttcggcacc agcacctttc tcggcgaata
     2221 cgctaaggtt gcgagtccga aggattttca gtttctgcgc tatgtgattt ccggcgcgga
     2281 gaaattaagc gaagaggtgg cgcgaacctg gctggagaaa ttcggactgc gcatcatgga
     2341 aggctatggc gccaccgaat gcgcgccggt gctggcggtc aacagcaacc tggcttatcg
     2401 tcgtggcgcg gtcggacggt tcctgcaggg aatcgaattc cgcctgcaaa aaatacccgg
     2461 catcgggcac ggcagcttgc tgcatgtgcg cggcccgaac gtcatgctcg gctattacct
     2521 gcaggagcgg ccgggcgagc tgcaatccac gcattccgaa ttcggccccg gctggtacaa
     2581 caccggcgac g
//
LOCUS       DATIOW010000189         1651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1405625, whole genome shotgun sequence.
ACCESSION   DATIOW010000189 DATIOW010000000
VERSION     DATIOW010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405625"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..753
                     /locus_tag="VLL03_06300"
     CDS             100..753
                     /locus_tag="VLL03_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HSS47004.1"
                     /translation="MDFEQARFNMIEQQIRTWEVLDQEVLDLLSVIKRERFVPEKWRA
                     LAFADMEIPLGHNQAMWPPKLEARCIQELGLKKTDTVLEVGTGGGYLTALLAARAQHV
                     CSVEIYPELAKRAAHTLKTEGVANVTLDTGDASRGWNKFAPYDAIVLTGSVPLLIEVY
                     QQALKPEGRLFVIVGDPPVMVAKLISCAAPGAYSSVDLFETCVAPLVNAPQPERFVF"
     gene            complement(950..>1651)
                     /locus_tag="VLL03_06305"
     CDS             complement(950..>1651)
                     /locus_tag="VLL03_06305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSS47005.1"
                     /translation="AVQTQGDAGRLRTLGALAVEVTGSLKFDVMPPPSAYDQGRELRA
                     RFGTRLVLLAASTRVGEEERVLQAVSRAGVPGLLTVIVPRHPQRFDEVAALLERRGIR
                     FQKRSENLPVAADTQIVLGDSMGEMFAYYAACDVAFVGGSLLPLGGQNLLEACAMGKP
                     VLIGPYTFNFAEAAELAVTAGAAQRVADAAELGSALKSLFADEVRRKAMGEAGRAFCA
                     AHQGATQKILRLIRF"
BASE COUNT          354 a          510 c          448 g          339 t
ORIGIN      
        1 gtgatgactc gcaccacaag gacccctaac gtgtatccgt caagctactt caactatgcg
       61 ataattgcaa gctcgcaaac acctcccgat ttcagtccca tggatttcga gcaagcgcgc
      121 ttcaacatga tcgagcagca aatccgcacg tgggaagtac tcgaccaaga agtgcttgat
      181 ttgttatctg taatcaagcg cgagcggttc gttccggaaa agtggcgcgc gctggccttt
      241 gccgacatgg aaatacctct ggggcataac caggcgatgt ggccgccgaa actggaagcg
      301 cgctgtattc aggaactcgg actaaaaaaa accgacacag tgctggaagt gggaaccggc
      361 ggcggttatc tcaccgcgct tctcgccgcc agagcgcagc atgtctgcag cgtggaaatc
      421 tatcccgagc tggcgaaacg agcggcgcac actctcaaaa ccgagggtgt ggccaacgtc
      481 acgctggata ccggagatgc ttcgcgaggc tggaacaaat ttgcgcctta tgatgcgatt
      541 gtgctcaccg gctcggtgcc gctgctcatt gaagtttacc agcaggcctt gaagccggaa
      601 ggcaggctgt ttgtcatcgt cggcgatccg ccggtgatgg tggcaaaact catttcctgt
      661 gccgcccccg gcgcgtatag ttcagtggat ctctttgaaa cctgcgtcgc gccgctggtt
      721 aatgcgccgc agcccgaacg tttcgttttc tagtcattgc gagcgtagca aacttatcaa
      781 ccgcagatga acgccgatga acacggatca aacccaccgc gccctgaatt ttatcggcgt
      841 ctatcggcgt tgatcggcgg ttaagtgttc tggtcagccg acgtagcaat ctgtttttct
      901 accaaacgca tcacccatta cccattactc atcacccatg tttcccggcc taaaagcgaa
      961 tcaggcgcaa aatcttttgt gtcgcgccct ggtgcgcggc gcaaaacgcg cggcctgctt
     1021 ctcccatcgc ttttcttctc acttcatccg caaacaacga tttgagcgca ctcccaagct
     1081 cggcagcatc agccacgcgc tgcgccgcgc ccgctgtaac cgcaagctct gcagcttcgg
     1141 caaaattgaa cgtgtacggg ccgattaaca ccggcttgcc catggcgcag gcttcaagca
     1201 gattctgtcc gcccaaaggc agaaggctgc cgcccacgaa agccacatcg cacgccgcgt
     1261 agtaggcgaa catttcgccc atgctgtcgc ccagcacaat ttgcgtgtct gcggccaccg
     1321 gcaggttttc gcttcttttc tgaaagcgga tgccgcgacg ctctaaaagc gcggctactt
     1381 cgtcaaagcg ctgcggatgg cgtggcacga taacggtaag cagcccggga acgccggcgc
     1441 gggaaaccgc ttgcagcacc cgctcttcct cgcccacgcg ggtgctggct gcgagcaaca
     1501 ccagccgtgt gccaaagcgc gcgcgcagtt cccgtccctg atcgtaagcg gatggcggcg
     1561 gcatcacatc aaacttgagg ctgcccgtta cttcgactgc cagggcaccg agtgtgcgca
     1621 aacgcccggc atcgccttgc gtttgcaccg c
//
LOCUS       DATIOW010000190         5187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_694338, whole genome shotgun sequence.
ACCESSION   DATIOW010000190 DATIOW010000000
VERSION     DATIOW010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694338"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VLL03_06310"
     CDS             complement(<1..612)
                     /locus_tag="VLL03_06310"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HSS47006.1"
                     /translation="MNANPKFLSATAYVDAAAVKPLPNSRKVYVEGSRPDIRVPMREI
                     TQSDTPASFGAEKNPAVYVYDTSGPYTDPEVSVDIRAGLPALRRKWILGRNDTEKLSG
                     PSSEYGRSRLTDPELAELRFNLKCKPLRAKPDMNVTQMHYARRGIITPEMEFIAVREN
                     QHRDELSELLTRQHPGQDFGASIPKFITPEFVRDEVARGRAIIP"
     regulatory      complement(652..818)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            881..1534
                     /locus_tag="VLL03_06315"
     CDS             881..1534
                     /locus_tag="VLL03_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HSS47007.1"
                     /translation="MDFEQARFNMIEQQIRTWQVLDQEVLDLLFVIRRERFVPEKWRM
                     LAFADMDIPLGHSQAMWPPKMEARVLQELAPKKTDTVLEVGTGSGYLTALFAAKAQHV
                     YSVEIIPELSKQAAQTLRSEGISNVTLEVGDAARGWNAHAPYDVIVLTGSVPMLPEAW
                     QQSLNPGGRLFAIVGDPPVMAARLINCVAPGACNSVALFETCIAPLINAPQPERFVF"
     gene            1830..2057
                     /locus_tag="VLL03_06320"
     CDS             1830..2057
                     /locus_tag="VLL03_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HSS47008.1"
                     /translation="MSYRERIVIDPAVRSGKPCIKGTRITVYDILEYLAGGMSQDDIL
                     RDFPSLSQEDIRAALEFAADRERKLASPIAG"
     gene            2054..2410
                     /locus_tag="VLL03_06325"
     CDS             2054..2410
                     /locus_tag="VLL03_06325"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HSS47009.1"
                     /translation="MKLLFDENLSSQLIELLSADFPGSTHVEHLGMRGATDAQIWGCA
                     RDRGYMIVSKDNDFRQYAFLYGPPPKIIWLNVGNAGTTAIARLVSSRIAEIRAFDKDP
                     ELSLLPLDLEQKIITP"
     gene            complement(2657..3904)
                     /gene="waaA"
                     /locus_tag="VLL03_06330"
     CDS             complement(2657..3904)
                     /gene="waaA"
                     /locus_tag="VLL03_06330"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF004386.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HSS47010.1"
                     /translation="MIKTRFVYTLVTSAMLPFALLHLLWRSRRQPAYLKHIPERFGFY
                     REASGKPVIWLHAVSVGETRAAEPLVKALRQAHPGHQILLTHMTPTGRETGERLFGGN
                     VLRCYLPYDFPQAVKRFLAHFKPVAGVLLETEIWFNLIYLCKEKGIPLYLVNARLSEK
                     SAAGYARFANLVRPALQSLTTVSAQTQQDAERLRALGAKAVQVTGNLKFDMLPPFFAL
                     EVGRELHSGFGARPVFLAASTREGEEELVLQAAAATGIADLLTVIVPRHPQRFDEVAA
                     LLERRGIQFQRRSENRLVAADTQVVLGDSMGEMFAYYVACDLAFVGGSLLPYGGQNLL
                     EACAVGKPVLIGPHTFNFAEAAELAVQAGAAQRVTSAAELGEAIKTLFANAVRRKAMG
                     EAGRVFCAAHQGATQRILRLLRF"
     gene            4041..>5187
                     /gene="waaA"
                     /locus_tag="VLL03_06335"
     CDS             4041..>5187
                     /gene="waaA"
                     /locus_tag="VLL03_06335"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF004386.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HSS47011.1"
                     /translation="MIKTRFVYTLATYAMLPFALLYLLWRSRRQPAYLKHIPERFGFY
                     REAPGKPVIWLHAVSVGETRAAEPLVKALREAHPGHQILLTHMTPTGRETGERLFGGN
                     VLRCYLPYDFSQAVESFLRHFKPVAGVLLETEIWFNLIRLCKEKGIPLYLVNARLSEK
                     SAARYSRFRSLVSAGLNELTAIAAQTQDDAERLRTLGAKTVQVTGNLKFDMLPPFFAL
                     EVGRELHSGFGARPVFLAASTREGEEELVLQAAAATGIADLLTVIVPRHPQRFDEVAA
                     LLQNRNVRFQRRSDNRPVTANTQVVLGDSMGEMFAYYAACDVAFVGGSLLPLGGQNLL
                     EACAMGKPVSIGPHTFNFAEAAELAVQAGAAQRVTSAAELGEAIKTLF"
BASE COUNT         1080 a         1517 c         1487 g         1103 t
ORIGIN      
        1 gggaataatc gcccggccgc gcgctacttc atcgcgcacg aattccggcg tgatgaattt
       61 gggaatagag gccccgaaat cctgccccgg atgctggcgg gtaagcaatt cggaaagctc
      121 gtcgcggtgc tggttctcgc ggacggcgat gaattccatt tccggggtga tgatgccgcg
      181 ccgagcgtaa tgcatttgtg tgacattcat gtccggcttg gcgcgcagcg gcttgcactt
      241 caaattgaaa cgcagttccg ccagctcagg atcggtcagg cggctgcggc cgtattcgga
      301 gctggggccg gagagttttt ccgtgtcatt gcgcccaagt atccatttcc ggcgcagggc
      361 aggaagcccg gcgcggatgt ccacagacac ttccggatca gtgtaaggcc cggaagtgtc
      421 atagacataa accgccgggt ttttttccgc gccgaaagaa gcgggcgtgt cggattgagt
      481 aatttcgcgc atcggcaccc ggatgtcggg tctgctgcct tcaacataaa ctttgcgcga
      541 attaggtaaa ggtttcaccg ccgctgcatc aacgtaagcg gtggcgctta agaatttcgg
      601 gttggcattc actgtttgct cctcgcagga cacaaggagc attggttggg cttgaatcaa
      661 tcaacgcttc cctgcgccgg cattatccgt atcaggttcg aagggtattc tctcaccggt
      721 cggagcgttc agttatcagc tttcagccat cagcaaacat tcgctgaccg ccgaccgctg
      781 agggctgacc gctgcaacgc ggacccctaa cgtgtaaccg tcaagctact tcaactatgc
      841 gataattgca agttcgcaaa cgccctccga tttcagacat atggatttcg aacaagcgcg
      901 cttcaacatg attgagcagc aaatccgcac gtggcaagtg ctcgatcagg aagtgcttga
      961 tttgttgttc gtaatcaggc gcgagcggtt tgtgccggaa aagtggcgca tgctggcttt
     1021 tgccgacatg gatatcccct tggggcacag tcaggcgatg tggccgccga aaatggaagc
     1081 gcgcgtgctg caggagcttg cgccaaaaaa aaccgatacg gtgctggaag tcggcactgg
     1141 cagcggttac ctcactgcgc tcttcgccgc caaagcgcag cacgtctaca gcgtggaaat
     1201 cattcccgag ctttctaagc aggcggcgca aaccctccgc tccgaaggta taagcaacgt
     1261 cacgctggaa gtgggagatg ccgcgcgcgg ctggaatgcg catgcgcctt acgatgtgat
     1321 tgtgctcacc ggttcggtgc cgatgttgcc cgaggcgtgg cagcaaagcc tgaatcccgg
     1381 cggcaggctg tttgccatcg tgggcgatcc gccggtgatg gcggcaagac tgattaactg
     1441 tgttgcaccc ggagcgtgca actcggtggc tttgttcgaa acctgcattg cgccgcttat
     1501 caacgcgccc cagcccgagc gtttcgtttt ctagatcatt gcgagcgtag cgaacttatc
     1561 aaccgcagat gaacgccgat gaacgcggat caaacccacc gcgcccttgc tctcggcaaa
     1621 agaccgcgcc ggtgtccctt tgaagcaggc agaagtattc gactgaacgg ggcaaacaag
     1681 aggacaaatc ggatgcgcgg tgccgcatgc accatccgta ttttctgttc gctgctaccg
     1741 gcagctattc tccggcagcc gctgcccgac gcgcatgttg attcatacat tgccttgagt
     1801 tcaccatgcg cctgcggtaa cataaataca tgagttatcg agaacgcatc gtgattgacc
     1861 cggccgttcg cagcggcaaa ccgtgcatca agggcacccg cattacggtg tacgacatcc
     1921 tggaatatct cgcgggcggc atgtcgcaag atgacatcct gcgggacttt ccttccctgt
     1981 cgcaggagga tatccgggct gcccttgagt ttgccgccga tcgggaacgc aagctcgcgt
     2041 cacctattgc cgggtgaaac tgcttttcga tgaaaatctc agctcgcagc tcatagagct
     2101 tctctcggct gatttccctg gcagtactca cgtcgagcat cttggcatgc gcggcgcaac
     2161 agatgctcaa atttggggct gtgcgcgcga ccgcggttat atgatcgtgt ccaaggataa
     2221 cgacttccgg caatacgcat ttctctacgg gccaccgccg aaaattatct ggcttaatgt
     2281 cggtaacgca gggaccacgg ccatagcacg cttggtctcc agcaggatcg cggaaattcg
     2341 ggcgtttgat aaagacccgg agctgagcct gctacccctg gacctggagc agaaaatcat
     2401 caccccgtaa atggcgagcg aagtttttca ggtgctcgag agagttgcca gcttgtcacg
     2461 ggtttccatc gcaaagctgt tggcgcaata tccaagcacg gctagtaagg ggatcgtttt
     2521 tcaccttttc ccattcacca tataccattc cccattcact ggttacgact ccccatcact
     2581 cgtcactcgt tacccatcac ccggtcactt aatcactcag tcactatata attactcatc
     2641 acccatgttt tccggtctaa aagcgcagca gccgcaaaat tctttgcgtc gcgccctgat
     2701 gcgcggcgca gaaaacacgg cccgcttctc ccatcgcttt tcgcctcact gcattggcaa
     2761 acaaggtttt gattgcttcc cccagttcgg cagcactggt aacgcgctgc gctgcacccg
     2821 cttgcacggc aagctcggcg gcttcggcaa agttaaaagt atgcgggccg attaaaaccg
     2881 gcttgcccac cgcacaggct tcgagcaggt tctgaccgcc gtaaggcagg aggctgccgc
     2941 caacaaaagc caggtcgcac gccacatagt aagcgaacat ttcccccatg ctgtcgccca
     3001 gcaccacctg cgtgtcggcg gccaccagcc ggttttcgct tcttctctga aattgaatcc
     3061 cgcgccgctc taaaagcgcg gccacttcgt caaagcgctg cggatggcgc ggcacgatca
     3121 ccgtaagcaa atccgcaatg ccggttgctg cggccgcttg cagcaccagt tcctcctcgc
     3181 cttcacgggt gctggcggcg aggaacaccg gccgcgcgcc gaagccggaa tgcaattccc
     3241 gcccgacctc gagcgcgaaa aagggcggca gcatgtcgaa tttcagattg ccggtgactt
     3301 gaactgcctt cgcacccagc gcacgcaagc gttccgcatc ctgttgcgtc tgcgccgaaa
     3361 cagtggtcag cgactgtagt gccggccgca ccaggtttgc aaaacgcgcg tagcccgcgg
     3421 cggatttttc agagagccgg gcgtttacca gataaagcgg aatccctttt tccttgcata
     3481 ggtaaatcag gttgaaccag atttcggttt ccagcagcac gcctgcgaca ggcttgaaat
     3541 gcgcgaggaa acgcttgacc gcctgcggga aatcataggg aagataacag cgcagaacgt
     3601 tgccaccaaa aagccgctcg ccggtttcgc gcccggtggg cgtcatatgg gtgagcagaa
     3661 tctgatgtcc gggatgcgcc tggcgcaacg ccttcaccag cggttcggcg gcgcgcgtct
     3721 cgccgaccga aaccgcgtgc agccaaatga ccggttttcc ggacgcttcc cggtaaaatc
     3781 caaaacgctc gggaatatgc ttgagatagg cgggctggcg gcggctgcgc cacaggagat
     3841 gcagcagcgc gaaaggcagc atcgcagagg tgaccaaggt gtaaacgaag cgcgtcttga
     3901 tcatcgtccc ctcacctctt cggcgaaaga gctgaagcga gaatcactca tatcaatcct
     3961 ccctcccccg caagcggggg agggaagatt gatatgagtg actctcgctt cagctctttc
     4021 gccgaagagg tgaggggacg atgatcaaga cgcgcttcgt ttacaccctg gctacttatg
     4081 cgatgctgcc tttcgcgctg ctttatctgc tgtggcgcag ccgccgccag ccggcctatc
     4141 tcaagcatat tcccgagcgt tttggatttt accgggaagc gcccgggaaa ccggttattt
     4201 ggctgcacgc ggtttcagtc ggcgagacgc gcgccgccga accgctggtg aaggctttgc
     4261 gtgaggcgca tcccggacat cagattctgc tcacgcacat gacgcccact gggcgcgaaa
     4321 ccggcgagcg gctttttggc ggtaacgttc tgcgctgtta tcttccctat gatttctcgc
     4381 aggcggtcga gagttttttg cggcatttca aaccggttgc cggcgtgctg ctggaaaccg
     4441 aaatctggtt caacctgatt cgcctctgca aggaaaaagg gattccgctt tatctggtaa
     4501 acgccaggct ctctgaaaaa tccgcagcgc gttactcgcg ttttcgcagc ctggtgagcg
     4561 ccggcctgaa tgagctgacc gccattgccg cgcagacgca agacgatgcc gaacggctgc
     4621 gaacgctggg cgcgaagaca gttcaagtca ccggcaatct gaaattcgac atgctgccgc
     4681 cctttttcgc gctcgaggtc gggcgggaat tgcattctgg cttcggcgcg cggccggtgt
     4741 tcctcgccgc cagcacccgt gaaggcgagg aggaactggt gctgcaagcg gccgcagcaa
     4801 ccggcattgc ggatttgctt acggtgatcg tgccgcgtca tccacagcga tttgacgaag
     4861 tcgcggcgtt attgcaaaac cgtaacgttc gcttccagcg cagaagcgat aaccggccgg
     4921 tcaccgccaa tacgcaggtt gtactgggcg acagcatggg ggaaatgttc gcctactatg
     4981 cggcgtgcga cgtggctttc gtcggtggca gcctcctgcc tttgggcggg cagaatctgc
     5041 ttgaagcctg cgccatgggc aagccggttt caatcggccc gcatactttc aactttgccg
     5101 aagccgccga gcttgccgtg caagcgggtg cagcgcagcg cgttaccagt gctgccgaac
     5161 tgggggaagc aatcaaaacc ttgtttg
//
LOCUS       DATIOW010000191         9368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1405945, whole genome shotgun sequence.
ACCESSION   DATIOW010000191 DATIOW010000000
VERSION     DATIOW010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9368
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405945"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VLL03_06340"
     CDS             complement(<1..457)
                     /locus_tag="VLL03_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS47012.1"
                     /translation="MKAAVLLVLLAPLACFAGGSNYGIMPGTNPHFAGKVTEWPVPTP
                     KFARDPAPAPDGSIFITVMHGNKIARFDPKTQTFKEWDLPSGAQPHGLLVDKNGIVWY
                     TGNGNGSIGRLDPASGKVTEFKAPSGGDPHTLVIDDKGVIWFTVQSGNRV"
     gene            complement(465..1268)
                     /locus_tag="VLL03_06345"
     CDS             complement(465..1268)
                     /locus_tag="VLL03_06345"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HSS47013.1"
                     /translation="MTSAVTAATATKQLLLRHDSDSITTLTLNRPGQFNALSGALLND
                     LQAALDGIAKDQSVRVVIIAGAGPAFCAGHDLKEMRTNCNPAFIEDLFRRCSKVMTTI
                     TQIPQPVIARVHGIATAAGCQLVATCDLAVASTEARFATSGINVGLFCSTPGVALSRN
                     VLRKQAMEMLLTGDFIDAQTALTRGLVNRVVAPEQLNTEVMRLAEVITSKPRSAIALG
                     KQMIYKQLEMGLEPAYQYASGVMTCNMLDDDAAEGIDAFMQKRPPKWKS"
     gene            1506..2213
                     /locus_tag="VLL03_06350"
     CDS             1506..2213
                     /locus_tag="VLL03_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HSS47014.1"
                     /translation="MELFSAQFFSALFAIIIIDLVLAGDNAIVIALAARNVPKHLQKR
                     AILWGAAGAIAVRTAMTLIVVWLLQIPGLLLTGGLLLVWIAYRLLVPAEENRKDTEHA
                     AGSFWEAMKTIVIADAVMGLDNVLAVAGAAYGSFLLVIIGLLISVPIVVWGSTLILDW
                     INRFPALVYIGAAVLAWTAAKMIRDERMLEDFLNRHKPVVWAIYTLVIGGVLYAGYAV
                     NKRKKHAGSAIQTQLPD"
     gene            complement(2232..2558)
                     /locus_tag="VLL03_06355"
     CDS             complement(2232..2558)
                     /locus_tag="VLL03_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS47015.1"
                     /translation="MNRTTKSLVSLLVAVLMAASTGIVRADQYSDATKPTGEAIAGDI
                     ILVRPIGLAATILGTAIFVVSLPFSIPSGSVGSAAKSLIAEPAQYTFLRPLGQTQAKP
                     IGDVQR"
     gene            complement(2720..3268)
                     /gene="ppa"
                     /locus_tag="VLL03_06360"
     CDS             complement(2720..3268)
                     /gene="ppa"
                     /locus_tag="VLL03_06360"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HSS47016.1"
                     /translation="MNLDRVGPGRNLPDDFNVIIEIPMNADPIKYEVDKETGAVFVDR
                     FMSAAMHYPCNYGYIPRTLSADGDPVDVLVLSPVPLITGVVVRCRPVGMLKMTDEAGD
                     DAKLLVVPIDKLCNLYRAIQSPRDLPELTLSQIAHFFEHYKDLEPGKWVKLTGWAGPE
                     EAIAEILAGAQRYNSAAKKPMF"
     gene            3358..4467
                     /locus_tag="VLL03_06365"
     CDS             3358..4467
                     /locus_tag="VLL03_06365"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HSS47017.1"
                     /translation="MGHAFKYGHAAGKEWQETSRACMTQIGRVPPAANLGFLYVTDFF
                     VPHAGHILDFFRDHTGIAHWVGSVGVGVCGTGKEYLDQPAVAAMLTELPEASFRVFRD
                     VTASADDLPIMPGDSAAHFAIVHADPHTPEITTLVEDVAEAMQSGFLVGGLTSSRNRH
                     VQIADEVVQGGLSGVIFSDAVTISTRLTQGCSPLGPRHVVSECQRNVIIGIDGRPALD
                     VFKEDIGEVLARDLDRVSGHIFAGLPIEGSDIGDYVVRNLVGIDVNHKMLAIGEWLEP
                     GMPLIFCRRDGTTAHDDMQRMLKDIQRDLGAPPKGGLYFSCLGRGENMFGANSEELRL
                     IQNELGDFPLVGFFANGEISHNRLYGYTGVLMLFI"
     gene            4491..5096
                     /locus_tag="VLL03_06370"
     CDS             4491..5096
                     /locus_tag="VLL03_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HSS47018.1"
                     /translation="MKLYDRDVSGNSYKVRLFLALLGLPYETVTINTAKGEQKQPEFL
                     QLNPRGQIPVLEADGKAIWDSTAILVYLARRYGGEKWLPLEAHDMAEVVQWLALAQNE
                     ILYGLAYSRVIILFKRPVGNLSDCQARGQGALKVLERHLRQNEWLALGRLTIADLACY
                     PYVALAEEGGIPLDPYTSVLAWMKRIQMLPGYVTMPGLRLV"
     gene            5274..6092
                     /locus_tag="VLL03_06375"
     CDS             5274..6092
                     /locus_tag="VLL03_06375"
                     /inference="COORDINATES: protein motif:HMM:NF015892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSS47019.1"
                     /translation="MFWSILFVTATAYCALCGFIFLFQPRLVFFPQIGRNEYVTPVQA
                     GMAFETVKITVADTELDAWWVPARGPRGAVLFFHGNAGNISHRLDYLRLFNRLGFSTL
                     IFDYRGYGKSGGEPSETGMYQDAEAAWRYVTQTRQVAPASVVIFGESLGGAVAAYLAA
                     QVKPRALILTSAFTSLPELGAKLYPLFPVRLLSRFKYDTREYLRRVSCPVLITHSRDD
                     EIVPFAQGEALYSAANEPKQFLEIFGGHNTGLMVSEQVWFKGVQAFFNSLESKR"
     gene            6184..6840
                     /locus_tag="VLL03_06380"
     CDS             6184..6840
                     /locus_tag="VLL03_06380"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSS47020.1"
                     /translation="MSKRPESMSFPHQATVIVVHGLWVNSLIMRPLALRIEHCGYAVS
                     CFSYASVRATLTQNAARLAEFVAVLSASTVHFVGHSLGGLVVLQMLKQGADRRIGRIV
                     LMGTPCGGSHAARVLAKSGPGQWLLGLSMPVGEMHLAAPACHDLGVIAGDRGLGLGRF
                     LAPGLPHPNDGVVTVNETRIAGMKDHIVMRVSHSGMLFSSRAASQVCAFLTNGHFRQH
                     "
     gene            6847..7884
                     /locus_tag="VLL03_06385"
     CDS             6847..7884
                     /locus_tag="VLL03_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HSS47021.1"
                     /translation="MRKPFKWMLLLLPLLGGCANFGFYWQSVSGQMEIWKKEQHIETL
                     LADQSVTPELKLRLQSVLDIREFASRELGLPDNGSYRLYADLQRPYVLWNVFATPEFS
                     IRPKEWCFVIAGCVNYRGYFSEADAQAFADNLHREGDDVFVAGVPAYSTLGWFDDPVL
                     STFIYFPETEVARLIFHELAHQLVYVPDDSTFNESFATAVEETGVERWLDKNGNDELR
                     SAFAAAQVRKKDFIALMLQYRGQLQGLYASEAGEQQKRESKASILRKLKQEYAALKIK
                     WGSFSDYDRWFAQDLNNAHLASVAAYTQLVPAFQHLLAQQQGDLPAFYAAVKEIAKLP
                     KSERLARLEER"
     gene            7931..8707
                     /locus_tag="VLL03_06390"
     CDS             7931..8707
                     /locus_tag="VLL03_06390"
                     /inference="COORDINATES: protein motif:HMM:NF022673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HSS47022.1"
                     /translation="MFEPLRPAGLAFSKLTDWPTFDDYQRLLAGKGITNAAGLPLRFV
                     SQGPKSGKPEDKYEARIYLRGEVQVRSQNWHDLFNALVWLIFPRSKAALNRRHFLSAS
                     ESEEKGEKNRGPVRDALTLFDEGGVIVACSDSRLAQLLVDFKWKELFWVNRDAVIASM
                     KFFLFGHAIYEKALQPFTGVTGRAVIFPVAADFFAAALEQQLQSLDEKLAGYLNNPQH
                     FNHTADLAPVPILGIPGWNTENERESYYENEAYFRAGRKT"
     gene            8823..9140
                     /locus_tag="VLL03_06395"
     CDS             8823..9140
                     /locus_tag="VLL03_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS47023.1"
                     /translation="MTDIEVFQFFRTRGAVIGLAFDITGAVLVNVGVRTHLRQALLLE
                     RHVVEETIDDLGAPDLLARNEAFNRDRAAERVRASRLAWLGLVCFIIGFMLQAISAWP
                     KST"
     gene            9171..>9368
                     /locus_tag="VLL03_06400"
     CDS             9171..>9368
                     /locus_tag="VLL03_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS47024.1"
                     /translation="MRKRAQLISDVRHDGFKMSTLKYHLDEQEIPDGFQIFEERLEVA
                     GVGFRKEDAAAFVTAKDGWLEL"
BASE COUNT         1859 a         2546 c         2851 g         2112 t
ORIGIN      
        1 cgacgcggtt gccgctctgc accgtgaacc agatgacacc tttatcgtcg attaccagcg
       61 tatgcggatc gccgcctgaa ggtgctttga attcggtgac tttgccgctc gccggatcaa
      121 gccgtccgat tgagccgttg ccattgccgg tgtaccagac aatgccgttt ttatcgacga
      181 gcagcccatg cggctgcgcg ccgctgggta aatcccattc tttaaaagtt tgggtttttg
      241 gatcgaagcg cgcgatcttg ttgccgtgca tgacagtgat gaaaatgctg ccgtcgggcg
      301 cgggcgcggg atcgcgcgcg aacttgggcg tgggcaccgg ccattcggtg actttgccgg
      361 cgaaatgggg attggttccc ggcatgatgc cgtaattgga accgccggca aagcacgcaa
      421 gcggcgcgag caaaaccagc agaaccgcgg ccttcatgga cgggtcagct tttccatttg
      481 ggcgggcgtt tttgcatgaa ggcgtcgatg ccttccgccg cgtcgtcatc cagcatgttg
      541 cacgtcatca ccccgctggc gtattgatac gccggctcca gccccatttc cagctgcttg
      601 tagatcatct gcttgcccag cgcgatggcc gaacgcggct tggatgtgat gacttccgcc
      661 agacgcatga cttccgtatt gagttgttcc ggtgcaacga cgcggttgac caaaccccgc
      721 gtgagcgcgg tctgcgcatc gatgaaatcg ccggtgagca gcatttccat cgcctgcttg
      781 cgcaacacat tgcgcgacag cgctacgccc ggagtggaac agaacaggcc gacgttgatg
      841 ccggaagtgg caaagcgcgc ttcggtcgag gccacagcca gatcacaagt cgcaaccagt
      901 tggcagccag cggcggtagc gatgccatgc acccgcgcaa tcaccggctg ggggatctgc
      961 gtgatggtcg tcatcacctt gctgcagcgc cggaacaaat cctcaatgaa tgccggatta
     1021 cagttggtac gcatttcctt gagatcatga cccgcgcaaa acgcggggcc ggctcccgca
     1081 atgattacca cgcgcaccga ctgatccttg gcaatgccgt cgagcgcagc ctgcagatcg
     1141 ttgagcagcg cgccggaaag cgcgttgaat tgtccggggc gattgagcgt gagcgtggtg
     1201 atgctgtcag agtcgtgacg cagcaggagc tgtttggttg cggttgccgc ggtgacggct
     1261 gaggtcatgg aaagactccc ggaaggaaca ggacgaacat tatattacta cgaagcgggc
     1321 cttgcttttt agttcccgcc gtgcgaggat ttgcacgccc tgcaaagggg agtagctttt
     1381 aaggcggccg tgtgccgccg gcggcaaaac cgtcagcacg ggattacctc cccggttttg
     1441 ccggcaaccg gaacggttgt gagcgagacc tttgtttatt cgctgatacc gtggaggtag
     1501 tcgtcatgga attattctcg gcgcagtttt tttccgcgct tttcgccatc atcatcatcg
     1561 acctggtgct tgcgggcgat aacgccatcg tgatcgcgct ggcggcacgc aacgtgccga
     1621 agcatttaca gaagcgtgcc atcctctggg gcgctgcggg tgccatcgcg gtgcgcactg
     1681 cgatgacgct catcgtggtt tggctgctgc aaattcccgg cttgctgctc accggtggac
     1741 tgctgctggt ctggatcgct tatcgcctgc tggtgcctgc agaagaaaac cgcaaagata
     1801 cggagcatgc ggcgggcagc ttctgggagg cgatgaaaac catcgtgatt gccgacgccg
     1861 tgatggggct ggacaatgtg ctggcggtgg caggcgctgc gtatgggagt ttcctgctgg
     1921 tgattatcgg cttgctgatc agtgtgccca tcgtggtatg gggcagcacg ctcatcttgg
     1981 attggataaa tcgttttccg gcgctggttt acattggcgc cgcggtgctg gcctggactg
     2041 cggccaaaat gatcagagac gagcggatgc ttgaggattt tctaaaccgg cacaagcccg
     2101 tggtgtgggc aatttatacg ctggtgattg gcggggtgct gtatgccggc tatgccgtca
     2161 acaagcgcaa gaaacacgcc gggagcgcga tacaaacgca gctcccggat tgaaaaccgc
     2221 tgcattattt attaccgctg tacgtctcca atgggcttgg cttgggtctg gccgagcggc
     2281 cgcaaaaaag tgtattgcgc cggttctgca atgagagatt ttgccgcgct tccgacactg
     2341 ccgctgggta tgctgaaagg cagggaaacc acaaagatgg cggtgcccaa aatcgttgcg
     2401 gcgaggccta tcggccttac gagaataatg tctccggcaa tggcttcacc tgtgggcttg
     2461 gtggcatccg aatattggtc ggcacgaacg atgccggtgc tcgcggccat caacaccgcg
     2521 accaacagcg atactaggga cttggtggtt cgattcatga tcgtcctcct tgggctctcc
     2581 ttcaagtaaa acttgaggag tctgggttaa ctatagcctg aaacgaggct aattgccaat
     2641 aaagtaagga aaaccggcga ttctggcaag ttttgggtgg gcgcggggcg gctggtcctt
     2701 ctctgccgcg ttgcgttttc tagaacatcg gcttcttggc ggcggagttg taacgctgcg
     2761 cgcccgccag gatctccgct atggcttctt ccggcccggc ccagccggtg agcttcaccc
     2821 atttgccggg ttcaaggtcc ttgtagtgct cgaaaaaatg cgcgatttgc gaaagcgtga
     2881 gttcaggcaa atcgcgcggc gactggatgg cgcgataaag attgcacagc ttatcaatcg
     2941 gcaccaccag caactttgca tcgtcacccg cttcatcggt cattttcagc attccgacgg
     3001 gacggcagcg caccaccaca ccggtgatca acggcaccgg cgagagcacc agcacatcca
     3061 ccggatcgcc gtcggcggaa agcgtgcgcg gaatgtagcc gtaattgcac ggataatgca
     3121 tcgcggcgga catgaagcgg tcaacgaaaa ccgcgccggt ttccttgtcc acttcgtact
     3181 tgatgggatc ggcattcatt gggatttcga tgatgacgtt gaaatcgtcg ggcaggttgc
     3241 gccccgggcc cactcggtca agatttatgc gtgctcccgt ttttccccgt atgcgatgcg
     3301 gccattatag cggcgcgcct tgttgttgta cggacacagg cggataattc tttggctatg
     3361 gggcacgctt tcaaatacgg ccacgccgcg ggcaaagaat ggcaggaaac ctcacgcgcc
     3421 tgcatgacgc aaataggtcg ggtgccgcct gccgcgaacc tcggctttct ctacgtgact
     3481 gacttttttg tgccgcatgc aggccatatc ctggattttt tcagagacca caccggcatt
     3541 gcacactggg tgggctcggt gggcgtgggt gtatgcggca cgggcaagga gtatctggat
     3601 caaccggcgg ttgcagcgat gcttaccgag ttgccggaag cctcgtttcg ggtgttccgc
     3661 gatgtcacgg cgagcgcgga tgacttgccc atcatgcccg gcgattcggc ggcgcatttc
     3721 gcgattgtcc acgccgaccc gcacacgccg gaaatcacca ccctggtcga ggatgtagcg
     3781 gaggccatgc aaagcgggtt cctggtcggc gggctcacca gttcgcgcaa ccgccatgtg
     3841 cagatcgccg acgaagtggt gcagggcgga ttgtccggag tcattttttc cgatgccgtg
     3901 acaatctcca cgcgtctcac ccaaggctgc tcgccgctcg gtccgcggca tgtggtgagc
     3961 gaatgccagc gcaatgtcat catcggcatc gacggccgtc ccgcactgga tgtattcaag
     4021 gaagatatcg gcgaggtgct ggcgcgcgac ctcgaccggg tctccggcca catcttcgcg
     4081 gggctcccca tcgaaggctc ggacatcggc gattatgtgg tgcgcaatct ggtcggcatt
     4141 gatgtcaacc acaagatgct ggcgatcggg gaatggctgg aacccggaat gccgctgata
     4201 ttctgtcgcc gcgacggcac cacggctcat gacgacatgc agcgcatgct taaggacatt
     4261 cagcgcgatc tcggggcccc gcccaaaggc gggctgtatt tttcctgcct cgggcgcggt
     4321 gaaaacatgt tcggcgccaa ttccgaggaa ctgcgcctca tccagaacga gctcggtgat
     4381 tttccgctgg tcggtttttt tgccaacggc gaaatctcgc ataatcgtct ttacggctat
     4441 accggcgtgc tcatgctgtt tatttaaaat cacgtcaata aggaaatcta atgaagctct
     4501 acgaccgtga cgtgtccggc aacagctaca aagtccgctt gtttctcgcc ctgctgggct
     4561 tgccttatga aacggtgacc attaacactg ccaaaggcga acagaaacag ccggagtttc
     4621 tccagctcaa cccgcgcggc cagattccgg tgctggaagc ggacggcaaa gccatctggg
     4681 attcaactgc gattctggtg tatctggcgc gccgctacgg cggcgaaaaa tggctgccgc
     4741 tcgaggcgca cgacatggcg gaagtggtgc aatggctggc gctggcacaa aacgaaattc
     4801 tttatggcct ggcttactcc cgtgtcatca ttctgttcaa gcgcccggtg ggcaatttaa
     4861 gcgactgcca ggcgcgcggg caaggcgcgc tcaaagtcct tgagcggcat ttgcggcaaa
     4921 atgagtggct ggcgctgggg cggctgacaa tagccgatct cgcctgctac ccctatgtgg
     4981 cgctcgccga ggaaggcggc atcccgctcg acccctatac ctcggtgctc gcctggatga
     5041 agcgcatcca gatgttgccg ggttatgtga ccatgcccgg cctgcgcctg gtgtagccgc
     5101 ctttttcctg ttttcccctt ggtaaaaatg tgaacattgc gtgacgtgtt cgtgatgtgc
     5161 agtccgataa tgcgaagcgg gccatgccaa attgtagcgg agtttcacgg ccgggtagcg
     5221 tgctttacaa tgcgatgggt attgattgtt ccctttaagg agcggcggtg cgcatgttct
     5281 ggagcattct ttttgtcacc gcaacggctt attgcgcgct gtgcgggttt attttcctgt
     5341 ttcagccgcg cctcgtcttt tttccgcaaa ttggcaggaa cgagtacgtg acgccggtgc
     5401 aagccggaat ggcgtttgag acggtgaaaa tcacggttgc agacaccgaa ctcgacgcct
     5461 ggtgggtgcc tgcgcgcggg ccgcgcggcg cggtgctgtt tttccacggc aacgccggca
     5521 atatttcgca tcgtctcgat tatctgcgcc tgttcaaccg gctgggcttt tcgacgctga
     5581 ttttcgatta ccgcggctat ggcaagagcg gcggcgagcc gtcggagacg ggtatgtatc
     5641 aggacgccga agcggcgtgg cgttacgtga cgcaaacgcg gcaggttgcg cctgcttccg
     5701 tggttatttt tggcgagtcg ctgggcgggg cggtcgcggc gtatcttgcg gcgcaagtca
     5761 agccgcgcgc gctcatcctc acttcagcct ttacttcgct ccccgagctc ggggcgaaat
     5821 tgtatccgct gtttccggtg cggctgcttt cgcgttttaa atacgacacg cgcgaatatc
     5881 tgcggagggt ctcttgcccg gtactgatca cgcacagccg cgacgacgag atcgtgccct
     5941 tcgcccaggg tgaggcgctg tactccgccg cgaacgagcc aaagcagttt ctggaaattt
     6001 ttggcggaca caataccggg ctgatggtga gcgaacaagt gtggttcaag ggtgtgcagg
     6061 catttttcaa ttctttggag agcaaaagat gaacggcatg cgacggcaga tagtgcgcgc
     6121 caacagcgtg cggctcaccc gaggaaacgc cgggagctct gctcgcggaa ttgctgccgt
     6181 ttcttgagca agcgtcctga aagcatgtcg tttccccatc aggcaacagt tatcgtggtg
     6241 cacggcttgt gggtaaacag cctgattatg cgcccgcttg cgctacgcat tgagcattgc
     6301 ggttacgctg tgtcctgctt ttcctatgca tcggtgcgcg caacgctgac gcaaaatgcc
     6361 gcacgcttag cggaattcgt cgccgtcctg tcggcgtcca cggttcattt tgtcggccac
     6421 agtctgggcg gcctggtggt tctgcaaatg ctgaaacagg gtgctgaccg gcgcatagga
     6481 cgcatcgtgc tgatgggcac gccctgcggc ggcagtcatg ccgcgcgggt gctggcgaaa
     6541 tccggccccg ggcaatggct gctgggacta agcatgccgg tgggagaaat gcatctggcg
     6601 gcgcctgcgt gccatgacct gggcgtgatt gccggggacc gcggcctggg gctggggcgg
     6661 ttcttggcgc ccggcctgcc gcaccccaat gacggcgtgg tgacggtgaa tgagacgcgc
     6721 attgccggaa tgaaggacca tatcgtgatg cgggtgagcc attccggcat gttgttttct
     6781 tcccgcgccg ccagccaggt atgcgctttt ctcacaaacg gccatttccg gcaacactga
     6841 gcaataatga ggaaaccttt caaatggatg cttttgttat tgccgctttt aggcgggtgc
     6901 gccaatttcg gtttttattg gcaatcggtg agcgggcaga tggagatatg gaaaaaagaa
     6961 cagcatatcg agacgctatt agccgatcaa agcgtgacgc ccgaattaaa gctcaggctg
     7021 caaagtgtgc tcgatatccg cgagttcgcc agccgcgagc tggggctgcc cgacaacggc
     7081 agctaccggc tctatgccga tttgcagcga ccctacgtgc tgtggaacgt atttgccaca
     7141 ccggagtttt cgatcaggcc gaaagagtgg tgctttgtga ttgccggctg tgttaattat
     7201 cgaggctatt tttcggaagc ggatgcacag gcctttgccg acaatttgca ccgggaaggc
     7261 gacgatgtct ttgtcgccgg cgttcccgct tattccacgc tcggctggtt tgacgatccg
     7321 gtgctgagca ctttcattta ttttcccgaa acagaagtgg cgcggctgat ttttcatgag
     7381 cttgctcatc aattagtgta tgtgccggat gattcgacat tcaatgaatc cttcgccacc
     7441 gcggtggaag agacgggtgt ggagcgctgg ctggataaaa acggcaatga tgagttacgc
     7501 tccgcttttg ccgccgctca ggtcagaaaa aaggatttca ttgcgctgat gctgcaatac
     7561 cgcggccaat tgcaggggct ttacgcctcg gaagcgggcg aacagcagaa gcgtgaaagc
     7621 aaggcgagca tcctgcgcaa gctgaaacaa gagtatgctg cgctcaaaat aaaatggggt
     7681 agtttttcag actatgaccg ctggtttgcg caggacctca ataatgcgca tcttgcttcc
     7741 gtcgccgcct acacccagct cgtgcccgca tttcaacatc tgctcgcgca acagcagggc
     7801 gacctgccgg cgttctatgc ggcagtgaag gaaatagcga agctgccgaa aagcgagcgg
     7861 ctcgcacggt tggaggagcg ctgacttggt tgcgcccgga tgggccccgt cattcctgcg
     7921 gcgctcgccc atgttcgagc cgctcaggcc tgcagggctt gcgttttcga agctcaccga
     7981 ttggccgacc ttcgacgatt accagcgcct gctcgcagga aaggggatta ccaacgccgc
     8041 aggcttgccg ctgcgctttg tttcccaagg cccgaaatcc ggcaaaccgg aggacaaata
     8101 cgaagcgcgg atttatttgc ggggcgaagt gcaggtgcga agccagaact ggcatgactt
     8161 gttcaatgcc ctggtgtggc ttatttttcc tcgaagcaaa gccgcgctca atcgcaggca
     8221 ttttctgtcc gcgagtgaat ccgaagaaaa aggagaaaag aaccgggggc cggtgcggga
     8281 tgcgctcacg ctcttcgacg aaggcggagt gattgtggct tgcagcgatt cgaggctcgc
     8341 gcaattgctg gtggacttca aatggaagga gctgttctgg gttaatcgcg atgctgtgat
     8401 tgccagcatg aaattttttc tgttcggcca cgccatttat gaaaaagcct tgcagccctt
     8461 tacgggcgtc accggccgcg cagtgatttt tccggttgcc gcggatttct tcgccgccgc
     8521 gctcgaacag caattgcagt cgcttgatga gaaacttgcc gggtatctca acaatccgca
     8581 acatttcaac cacactgctg atcttgcgcc ggttcccata ctcggcattc cgggatggaa
     8641 taccgaaaat gagcgcgaat cgtattacga aaatgaagct tatttcaggg cggggagaaa
     8701 gacgtgagta aaatacatta tttggcttga aggatgacaa tcagatgcct agaatgacgc
     8761 cttataaggc cgcatttcgg cggtctactt tatgttagag cgcatccaaa agtagggggg
     8821 ttatgactga cattgaagtc ttccagtttt ttcgcactcg gggcgcggta atcggtctcg
     8881 ccttcgacat tacaggcgcc gtactcgtca acgttggtgt tcgcactcac ttgcgacagg
     8941 cactacttct tgagcgccac gtagttgaag agacgattga cgatctcggc gcgcctgacc
     9001 tcctggctag gaacgaggcg tttaaccgag atcgcgcggc cgaacgtgtt cgcgccagtc
     9061 ggttggcttg gttagggctt gtttgcttca tcattggctt catgcttcag gcgatctctg
     9121 cttggcctaa atcaacgtga tgcgctctaa gccctccatc tagagctgac gtgcgcaagc
     9181 gcgcgcagct gatttccgac gttaggcatg acggtttcaa aatgagcaca ctaaagtatc
     9241 acttagacga gcaagaaata cccgatgggt tccagatatt cgaggaacgc cttgaggtgg
     9301 cgggcgttgg ctttcgaaaa gaagacgcgg cagcctttgt taccgcgaaa gacggctggc
     9361 ttgagctt
//
LOCUS       DATIOW010000192         3006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_591870, whole genome shotgun sequence.
ACCESSION   DATIOW010000192 DATIOW010000000
VERSION     DATIOW010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591870"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /gene="rpoA"
                     /locus_tag="VLL03_06405"
     CDS             complement(<1..900)
                     /gene="rpoA"
                     /locus_tag="VLL03_06405"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HSS47025.1"
                     /translation="MQSSAFLKPRIIDVQTVSPLHAKVTMEPFERGYGHTLGNALRRI
                     LLSSMPGFAPTEVTINSVLHEYSTLDGVQEDVVDLLLNLKGIVLKLHNRSEAMLTLKK
                     HGEGLVTAGDIEGGHDVEIVNPDHVIAHLSPGGKLDMQIKVEMGRGYVPGTVRQLPEE
                     KRKIGNIVLDASFSPVRRVSYSVESARVEQRTDLDKLIIDIETNGAIDPEEAIRYAAR
                     ILVDQLSVFADLKGTTFEIEQPKAPQVDPILLRPVDDLELTVRSANCLKAENIYYIGD
                     LIQRSETELLKTPNLGRKSLNEIK"
     gene            complement(928..1554)
                     /gene="rpsD"
                     /locus_tag="VLL03_06410"
     CDS             complement(928..1554)
                     /gene="rpsD"
                     /locus_tag="VLL03_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HSS47026.1"
                     /translation="MARNLDAKCRQCRREGEKLFLKGEKCFTDKCSIDRRSYAPGQHG
                     QKNMRLSGYAVQLREKQKLRRIYGLLERQFRNTYQEAERRKGVTGETLLQLLESRLDT
                     VSYRMGFGASRCEARQIVRHNGIRVNGKRVNIPSFQLRPGDVIEVAEKAKGQLRIKGS
                     LEAAEARGFPEWLEVDVKSFKGVFKAKPQRTELPSTINESMVVELYSK"
     gene            complement(1568..1960)
                     /gene="rpsK"
                     /locus_tag="VLL03_06415"
     CDS             complement(1568..1960)
                     /gene="rpsK"
                     /locus_tag="VLL03_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HSS47027.1"
                     /translation="MVTKTNVRVRKKVKKNVAEGIAYIHASFNNTIVTITDRQGNALA
                     WATSGAAGFKGSRKSTPFAAQVAAEQAGRAAQECGVKNLEVRIKGPGPGRESAVRALN
                     AVGFKITSISDVTPVPHNGCRPPKKRRI"
     gene            complement(1973..2359)
                     /gene="rpsM"
                     /locus_tag="VLL03_06420"
     CDS             complement(1973..2359)
                     /gene="rpsM"
                     /locus_tag="VLL03_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HSS47028.1"
                     /translation="MARIGGVNIPNHQHAEVALTAIYGIGRSRARKICAAAGITLSAK
                     IKDLSDAEMDKLREQVGKHAVEGDLRREVSMNIKRLIDLGCYRGTRHKRGLPVRGQRT
                     RTNARTRKGPRKAVRLAKAPTETGKN"
     gene            complement(2416..2529)
                     /gene="rpmJ"
                     /locus_tag="VLL03_06425"
     CDS             complement(2416..2529)
                     /gene="rpmJ"
                     /locus_tag="VLL03_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HSS47029.1"
                     /translation="MKVQASVKKMCRKCKIVRRKRVVRVICSDPRHKQRQG"
     gene            complement(2607..2825)
                     /gene="infA"
                     /locus_tag="VLL03_06430"
     CDS             complement(2607..2825)
                     /gene="infA"
                     /locus_tag="VLL03_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HSS47030.1"
                     /translation="MAKEETIQMQGEITETLPNATFRVKLENGHVVLGHISGKMRMHY
                     IRILPGDKVTVELTPYDLTKARIVFRAK"
BASE COUNT          523 a          912 c          851 g          720 t
ORIGIN      
        1 ctttatttca ttgagcgact tgcggccaag attgggcgtc ttcagcaact cggtttcgct
       61 gcgctgaatc aagtcgccga tataataaat attttccgcc ttcaggcagt tcgccgaccg
      121 cacggtaagc tccagatcgt ccaccgggcg cagcagtatc gggtccacct gcggtgcctt
      181 cggctgctcg atttcaaagg tagtgccttt gagatcggcg aatactgaaa gctggtccac
      241 caggatgcgc gcggcgtagc gaatcgcttc ttcgggatcg atggcgccgt tggtttcgat
      301 gtcgatgatc agcttgtcga ggtcggtgcg ctgctcgacg cgcgcgcttt ccaccgaata
      361 gctcacgcgg cgcaccgggc tgaacgaggc atcgagcacg atgttgccga tcttgcgctt
      421 ttcctccggt aattgccgca cggttcccgg cacgtagccg cgccccattt ccaccttgat
      481 ttgcatgtcg agcttgccgc ccggcgagag atgcgcaatc acgtggtccg gattgacgat
      541 ttccacatcg tgcccgcctt cgatgtcacc ggcggtcacc aagccttcgc catgtttttt
      601 cagcgtcagc atggcttcac tgcggttgtg cagtttcagc acgatgccct tgagattgag
      661 caggaggtcc accacgtctt cctgcacgcc gtcgagggtg gagtactcgt gcagcacgct
      721 gttgatggtc acttcggtcg gcgcaaaacc cggcatcgaa gacagcagga tgcggcgcag
      781 ggcattgccc agggtatggc cgtaaccgcg ttcgaacggt tccatggtca ctttggcatg
      841 caaaggtgaa acggtttgca cgtcgatgat acgcggcttg agaaaagcgc tgctttgcat
      901 ggctgaaccc tctcgattgc ttacagacta tttcgaatac aattcgacga ccatgctttc
      961 gttgatggtc gacggcagct cggtacgctg cggcttggcc ttgaatacgc ccttgaagct
     1021 ctttacatcc acttccagcc attcgggaaa accgcgcgct tcggcggctt ccagcgaacc
     1081 cttgatgcgc agctgcccct tcgccttttc ggccacctcg atcacatcgc cgggacgcag
     1141 ctggaaggac ggaatattga cgcgcttgcc gttcacccga atgccgttgt ggcgcacgat
     1201 ctggcgcgcc tcgcagcgcg atgcgccaaa acccatgcga tacgagacag tgtccagccg
     1261 cgactccaaa agctgcaaca gcgtctcgcc ggtgacgccc ttgcggcgct cggcttcctg
     1321 ataggtgttg cggaactggc gttcgagcaa accgtaaatg cggcgcagct tctgcttctc
     1381 gcgcaattgc acggcgtaac cggaaagccg catgtttttc tggccgtgct ggccgggggc
     1441 gtagctcctg cggtcaatcg agcacttgtc ggtgaagcat ttctcgccct tcaaaaacag
     1501 cttctcgcct tcgcggcggc actggcggca ttttgcatca agatttcggg ccatgatttt
     1561 tcctgattta gatgcggcgc ttcttcggcg gccggcagcc gttgtgcggc accggggtca
     1621 cgtcggagat gctggtgatc ttgaagccta cggcgttgag cgcccgtacg gcggattcgc
     1681 gtcccgggcc ggggcccttg atgcgcactt cgaggttctt cacgccgcat tcctgcgcgg
     1741 cgcgtccggc ctgctcggcc gccacctgcg cggcgaacgg cgtactcttg cgcgagccct
     1801 tgaagccggc ggcgcccgaa gtcgcccatg ccaaggcgtt accttggcgg tcggtgatgg
     1861 tgacgatggt attgttgaac gaggcatgga tataagcgat gccttccgcc acgtttttct
     1921 tcaccttctt gcgcaccctg acgttggtct tagttaccat ttagcgttgt cctcagtttt
     1981 tgccggtttc ggttggggct ttggcgagcc gcacggcttt gcgcgggccc ttgcgggtgc
     2041 gcgcgttggt gcgcgtgcgc tggccgcgca ccgggagccc ccgcttgtgg cgcgtgccgc
     2101 ggtagcagcc aaggtcaatc agccgcttga tgttcatcga cacttcgcgg cgcaggtcgc
     2161 cctcgaccgc gtgcttgccc acctgctcgc gcagcttgtc catctcggca tcagacaggt
     2221 ccttgatctt ggcggaaaga gtaatgcccg cggcggcgca gattttccgc gcccgcgaac
     2281 ggccgatgcc gtaaatcgcg gtcagcgcga cttccgcgtg ctggtggttc ggtatgttta
     2341 cgcctccgat acgggccatg acgtcctcaa aaaacaaaag cgtgcgatta taaggttaat
     2401 cgtcatattg ttcaactatc cctgacgctg cttatgccgc ggatccgagc aaatcacgcg
     2461 caccacgcgc ttgcgccgga cgatcttgca cttgcggcac atctttttca ccgaagcttg
     2521 tactttcatt tttagctcct tagctttcag caaaaaccag ccctcaccca aactggccgc
     2581 tgatagctga ttgctgacca gcttgtttac ttcgcgcgga acacgatccg tgccttggtc
     2641 aaatcgtagg gcgtcaactc caccgtcact ttatcgcccg gcaggatgcg gatgtaatgc
     2701 atgcgcatct tgccggaaat atgccccagc acgacatggc cgttttcaag tttcacccgg
     2761 aacgtggcgt tgggaagagt ttccgtaatc tccccctgca tctgtatcgt ttcttccttt
     2821 gccatgggct gaacttcgtc tttatgctcc aataccgcgt tgctccgttt gtttgcgtcg
     2881 cttgcatact tcggccaagc tagcaaaaaa actcgcgcct tgtcttggcg caaaatactc
     2941 gtttatccgt gactcgagtt taatcatcct ttcttatccc aggtcagcgc accggcaagc
     3001 cgctgc
//
LOCUS       DATIOW010000193         3014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_85345, whole genome shotgun sequence.
ACCESSION   DATIOW010000193 DATIOW010000000
VERSION     DATIOW010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85345"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..1325
                     /gene="glyA"
                     /locus_tag="VLL03_06435"
     CDS             57..1325
                     /gene="glyA"
                     /locus_tag="VLL03_06435"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HSS47031.1"
                     /translation="MSFSIQHNIASIDPELWKAICAENQRQEDHIELIASENYASPAV
                     LQAQGTVLTNKYAEGYPGKRYYGGCEFVDIAEQLAIDRVKALFNAEFANVQAHSGSQA
                     NQAVFFAVLKPGDTILGMSLAHGGHLTHGAAVNLSGKIFKAVTYGLHPKTEEIDYDEV
                     ERLAKQHKPKMIIAGASAYALVIDWKRFREIADSVGAYFFVDMAHYAGLIAAGYYPNP
                     VGIADFVTSTTHKTLRGPRGGIILTSKEELAKAINSIIFPGIQGGPLMHVIAAKAVAF
                     KEAQSREFKHYQKMVIDNARVMVKVLKKRGLRIVSGRTDCHLFLVDLRAKKITGKDAE
                     AALGRAHITVNKNAIPNDPEKPFVTSGIRIGTPAMTTRGFQEIEAEQLANLIADVLEA
                     PNDEKTLKRVAGEAKAMCAKFPVYGKKGKE"
     gene            1404..1883
                     /gene="nrdR"
                     /locus_tag="VLL03_06440"
     CDS             1404..1883
                     /gene="nrdR"
                     /locus_tag="VLL03_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HSS47032.1"
                     /translation="MKCPFCGAQDTQVVDSRVNEEGTSVRRRRRCISCTKRFTTYETV
                     ELRMPQIIKQNGNRTEFDRNRLHTGFMRALHKRPVAAKRVDEAIDRIMQKLLSLGERE
                     VPSRHIGEMVMKELYKMDKVAYIRFASVYRSFQDEEDFRDAIKEVQRTAKSRPRRRD"
     gene            1912..2997
                     /gene="ribD"
                     /locus_tag="VLL03_06445"
     CDS             1912..2997
                     /gene="ribD"
                     /locus_tag="VLL03_06445"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HSS47033.1"
                     /translation="MFSPDDHRYMAQALRLAEQGLYTTSPNPRVGCIIVREGKVIGQG
                     WHERAGQPHAEINALNVAGEKARGATVYVTLEPCNHHGQTGPCVEVLIKAGVAHVVAA
                     MQDPNPLVNGGGLEKLKQAGVQTECGILENEARELNIGFISRMAHGRPWVRMKIAASL
                     DGKTALNNGKSRWITGEPARQDAHHWRARSCAVLTGIGTIKDDDPQLTVRHVKTPRQP
                     LRVVVDSKLEILPTARVLQDGGVLIFCAIDDAARISRLRDVGAKVIILPNQEGKVELN
                     QMMASLAKFEVNEVLVEGGCRLNGSLLKAGLVDELLLYLAPHLLGDNARGMFDLPELT
                     ELTGRRELKILDLRMVGQDIRMLARFA"
BASE COUNT          728 a          818 c          891 g          577 t
ORIGIN      
        1 gccgaggcta acatggtaaa atcagacctt taatttaccg taagaaagcc gcgaccatgt
       61 ccttttccat acaacataat atcgccagca ttgatcctga gttgtggaag gcgatttgcg
      121 cagaaaatca gcgccaggaa gaccatatcg agctgattgc atcggaaaat tacgcgagcc
      181 cggcagtgtt gcaggcgcaa ggcacggtgc tgaccaacaa gtatgccgaa ggctatcccg
      241 gcaaacgcta ttacggcggc tgcgaattcg tggatatcgc cgagcagctg gcgatagacc
      301 gcgtgaaagc gctgttcaat gccgagttcg ccaatgtgca ggcgcattcc ggttcgcagg
      361 ccaaccaggc ggttttcttt gcggtgttga agccgggcga caccattctc ggcatgtcgc
      421 tcgctcacgg cgggcacctc actcatggcg ccgcggtcaa cctcagcggc aaaatcttca
      481 aagcagtaac ttatggcttg catcccaaaa ctgaagaaat cgactacgac gaagtggaac
      541 gtctggcaaa gcagcacaag cccaaaatga ttatagccgg cgcgtcggcg tacgcgctgg
      601 tgatcgattg gaagcgcttc cgtgaaattg ccgacagcgt tggcgcatat ttcttcgtcg
      661 acatggcgca ctacgcggga ttgattgctg caggttatta ccccaacccg gtgggtattg
      721 ccgattttgt caccagcaca acgcacaaga cgctgcgcgg cccacgcggt ggcatcatcc
      781 tgaccagcaa agaagaactc gccaaggcga ttaattccat catttttccg ggaattcagg
      841 ggggtccact gatgcatgtc atcgccgcca aggcggtggc attcaaggaa gcgcagagcc
      901 gcgaattcaa gcactaccag aaaatggtga ttgacaacgc gcgggtaatg gtgaaagtac
      961 tgaaaaagcg cgggctgcgc attgtctcgg gccgcaccga ctgtcatcta ttcctggtgg
     1021 acctgcgcgc caagaaaatc accggcaagg atgcggaagc cgcgctggga cgcgcgcata
     1081 tcacggtcaa caaaaacgct atccccaatg atccggagaa accttttgtc accagcggca
     1141 tccgcatcgg cacgccggcg atgaccacac ggggttttca ggaaatcgaa gccgaacaat
     1201 tggctaacct gattgccgat gtactggagg cgcccaacga cgaaaaaaca ttgaagcgcg
     1261 tcgcgggaga ggcgaaagcg atgtgcgcga agtttccggt gtatgggaag aagggtaagg
     1321 agtaaggggt caggagtgag gtgtaaaaac caccctaatc ttgtctcctc acccctcacc
     1381 ccttactact cacggggttc atcatgaagt gcccgttttg cggcgcgcaa gacacccagg
     1441 tggttgattc gcgggtgaac gaggaaggca ccagcgtacg ccggcgccgg cgctgtattt
     1501 cctgcacgaa gcgcttcacc acttacgaaa ccgtcgaatt gcgcatgccg caaatcatta
     1561 agcaaaacgg caaccgcact gaattcgatc gcaaccggct gcacaccggc ttcatgcgcg
     1621 cgctgcacaa gcgtccggtt gcagcgaagc gggtggatga agcgatagac cgcatcatgc
     1681 aaaagctgtt aagtctcggc gagcgcgaag tgccatcacg gcacatcggc gaaatggtga
     1741 tgaaggagtt gtacaaaatg gacaaagtgg catatatccg ctttgcctcg gtgtaccgca
     1801 gtttccagga cgaggaagat ttccgcgacg ctatcaaaga agtgcagcgg accgccaaga
     1861 gccggccgcg ccgccgcgat tgaatcattt caccgcgggt ttaacctcgt gatgttttcc
     1921 ccagacgatc accgttacat ggcgcaagcc ttgcgtctcg ccgagcaagg tttgtacacc
     1981 accagcccga atccgcgcgt gggctgcatc attgtgcgcg aaggaaaagt gatagggcag
     2041 ggttggcacg agcgcgccgg tcagcctcat gcggaaatca atgccctcaa tgtggcagga
     2101 gaaaaagcac gcggcgcaac ggtgtatgtc acgctggagc cttgcaatca tcatgggcag
     2161 accggccctt gcgttgaggt tctgattaaa gccggcgttg cgcacgtggt cgcggcgatg
     2221 caagacccca acccactggt caacggcggc ggattggaaa aactcaaaca ggccggcgtc
     2281 cagaccgaat gcgggattct ggaaaacgaa gcgcgcgaat tgaacatcgg ctttatctcg
     2341 cgcatggcgc acggccgtcc ctgggtaaga atgaaaatcg ccgcaagcct agacggcaag
     2401 accgcgctca acaacggcaa gagccgctgg attaccggcg aaccggcaag gcaggatgcg
     2461 caccactggc gcgcccgttc ctgcgcggta ctcaccggca tcggcaccat caaggacgac
     2521 gacccgcaac ttacggtgcg gcacgtgaaa acgccgcggc agcccctgcg cgtggtagtg
     2581 gacagcaagc tggaaattct tcccacggcg cgtgtgctgc aagacggtgg cgtcctgatc
     2641 ttctgtgcga ttgatgatgc agccagaatt tccaggttaa gggatgttgg cgccaaggtg
     2701 attattttgc caaaccagga aggcaaggta gagctgaatc agatgatggc gtcgctcgca
     2761 aaatttgagg tgaatgaagt cctggtggag ggcggctgca ggcttaacgg ttcgcttttg
     2821 aaagcgggac tggtggacga gttgctgctt tacctcgccc cgcatttgct tggcgataat
     2881 gcgcgcggca tgtttgattt gcccgagtta actgaattga ccggacgacg cgagttgaag
     2941 atcctcgacc tgcgcatggt agggcaggat attcgcatgc tggcgcgctt tgcctaggaa
     3001 tcctgtagcg ggca
//
LOCUS       DATIOW010000194         6995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_798421, whole genome shotgun sequence.
ACCESSION   DATIOW010000194 DATIOW010000000
VERSION     DATIOW010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6995
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798421"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VLL03_06450"
     CDS             <1..289
                     /locus_tag="VLL03_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HSS47034.1"
                     /translation="VAVGVLLTLALLEVIKLGIYLLIFVIIVQVLFSWVNPDAPLAPL
                     FNSLARPFLRPFRRLIPPIGNVDLSPLFVLVLAQLALILIAHGYRVIADMF"
     gene            complement(508..972)
                     /locus_tag="VLL03_06455"
     CDS             complement(508..972)
                     /locus_tag="VLL03_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS47035.1"
                     /translation="MKKELMTAQEIHDFGIEIVFKQLQKEGHKILSVNIELGTNPQIV
                     ARKDGQIEFIIVRTACYPNRGEIESAELALQCVAYADKADAICYFASVGIVNADGKTE
                     TEMGTPVKGVGFHAMYEGLLILTRSDRVKVWGPHGSSRLTPDDVNGKGRRKH"
     gene            complement(2508..3962)
                     /gene="rng"
                     /locus_tag="VLL03_06460"
     CDS             complement(2508..3962)
                     /gene="rng"
                     /locus_tag="VLL03_06460"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HSS47036.1"
                     /translation="MSEDILINVTPQETRVAVMGQGVVQELHIERVSGRGLVGNIYFG
                     EVCRVLPGMQSAFVEIGLDRAAFLHVADIWGQWQNGEEPKPIERLLHEGQKLLVQVIK
                     DPIGSKGARLSTQISAAGRMLVYLPQETHIGISQRIEDEEERAHLRDKLQQLLPQDQS
                     GGFIIRTMAETASERELLADVEYLRKLWSGIREQAQSAPPRTLLYQDLTLSLRVLRDF
                     VSEDSERVVVDSRETFHKMQSFAQNYMENMVPRIEHYTGERPLFDLHGVEEEIQKALA
                     RRVELKSGGYLIIDQTEALTTVDVNTGGFVGGRNFDDTIFKTNLEAAHVIARQLRLRN
                     LGGIIIIDFIDMDSEEHRNAVLAEFRKALARDRTRMTVNGFTALGLVEMTRKRTRESL
                     AHVLCEPCPTCEGRGEVKTAQTVCYEILRELLREARQFNTREYRILASQDVIDLFLDK
                     ESQSLAMLGDFIGKPVSLQVETQYTQEQYDIVLM"
     gene            complement(3959..4579)
                     /locus_tag="VLL03_06465"
     CDS             complement(3959..4579)
                     /locus_tag="VLL03_06465"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HSS47037.1"
                     /translation="MKRPDQHLYLASRSPRRRELLKQIDVNFEVVLFREYLVRGADVD
                     ESPHPGEPPGDYVKRIVCAKAEAGWSSIIRRRLRHFPVLAADTTVALGGKIIGKPENR
                     EHAVRMLKELSGQIHQVLTAVAMKLEDRLELRLSVTSVRFRELKDQEILNYVATGEPL
                     DKAGGYAIQGLAAAFIAEIDGSYSGIMGLPLFETAELMREFGYQVL"
     gene            complement(4588..5058)
                     /gene="rlmH"
                     /locus_tag="VLL03_06470"
     CDS             complement(4588..5058)
                     /gene="rlmH"
                     /locus_tag="VLL03_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HSS47038.1"
                     /translation="MKLWIIAVGDKMPAWVGTGFSEYAKRMPQEARVQLIEIKPEKRG
                     AGKNIQQVLEAEKGRILAALPRHCRRVVLDEKGKALSTAQLAQSLARWMQAGRDVGFV
                     LGGADGLDNEIKTSADMLLGLSAMTLPHALARVVLAEQLYRAVSLLHNHPYHRR"
     gene            complement(5070..5432)
                     /gene="rsfS"
                     /locus_tag="VLL03_06475"
     CDS             complement(5070..5432)
                     /gene="rsfS"
                     /locus_tag="VLL03_06475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HSS47039.1"
                     /translation="MRIDRMVKATVAALEDIKARDIMVLDVRKMTWLCDRMIIASADS
                     GRQSKALSSNVQEKLKALGAKVYGVEGEENGDWVLVDFGNVVVHIMQPAVRTYYNLEQ
                     LWGGEHKPVRRRRKAAAV"
     gene            complement(5429..6085)
                     /gene="nadD"
                     /locus_tag="VLL03_06480"
     CDS             complement(5429..6085)
                     /gene="nadD"
                     /locus_tag="VLL03_06480"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HSS47040.1"
                     /translation="MNALNPIGILGGTFDPLHYGHLTLAAEVAEQLNLAEVRFIPAGT
                     PPHRPAPFASSKARVDMLQLAISGNPRFSVDQREISRERASYTVDTLNELRQEVGASR
                     PLCLLLGTDAFAGLATWRRWEELFNLAHVAIALRPGFSLKDLPPVLAAEFAKRRAPSP
                     RTAVSSPCGSIVVLEITPVDISASIIREQLAAGATVRDLLPPAVLDYIRINHLYTRGG
                     "
     gene            complement(6085..>6995)
                     /locus_tag="VLL03_06485"
     CDS             complement(6085..>6995)
                     /locus_tag="VLL03_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HSS47041.1"
                     /translation="PERTHLPHASPSVRRFAREMGVNLDQVGGSGPKSRILKEDVQAY
                     VKVELSRPRGGAGFALPEMPQVDFAKYGPIESKPLSRIKKLSGANLHRNWVSIPHVTQ
                     HDEADITDLEAFRKSNSAETEKQGFKLTMLSFLIKACVTALKVHPEFNASLDKSGENL
                     VLKKYYHIGVAVDTPDGLVVPVIRDADRKGVFDLARQLAEISKRARDKKLSPAEMQGG
                     CFSISSLGGLGGTAFTPIINAPEVAILGVSKAQLKPVYQNGQLVPRLLLPLSLSYDHR
                     VIDGAAAARFTTYLCSVLSDIRRLLL"
BASE COUNT         1438 a         2084 c         1893 g         1580 t
ORIGIN      
        1 ggtcgctgtc ggcgttctgc tgaccctggc cctgctggaa gtgattaagc tcggtattta
       61 cctactgatc ttcgtgatca ttgtgcaagt gttgttcagc tgggtaaatc ccgatgcgcc
      121 gctggcgccg ctgttcaaca gcctggcccg gccgttcctg cgcccgttcc ggcgcctgat
      181 tcccccgatt ggaaacgtgg atctgtcgcc gctgttcgtc ctggtgctgg cgcaacttgc
      241 gttgattctc atcgcccacg gctatcgcgt gatcgcagac atgttttgat tgaccccttc
      301 tgagccgccg ggggccgcaa ggggatgaag ctcggcactg gttggagcac gttctgttac
      361 ccaaatggct ccccaagcaa gacaaatctg ggcactggtt ggagctgatt cgttttcgct
      421 aacgtaacgg ctcaaccggt gtgaggcaag caacatgaga ataagcgaag cctcgccgcc
      481 tgtggagcgt cccggttaaa gcgcaggtta gtgctttcgc cgacctttgc cgttgacatc
      541 atcgggggtg aggcgactgg atccatgcgg cccccagact ttgacccggt cagagcgagt
      601 gaggatcaaa agtccctcat acattgcgtg aaatccgacc ccctttaccg gagtgcccat
      661 ttctgtttcg gtttttccat ctgcgttcac aataccgaca ctcgcaaagt agcatatggc
      721 atcggcttta tcggcatatg caacgcactg aagggctaac tcggcactct cgatttctcc
      781 cctgttggga tagcacgcag tgcgaacaat gatgaactca atctgaccgt cctttctagc
      841 gacgatctgt ggattcgttc ctaattcaat gttaacagaa agaatcttgt gaccttcttt
      901 ctggagttgt ttaaacacga tctcaatacc gaaatcgtgg atttcctgcg cagtcatgag
      961 ctcttttttc ataaaacgat ttccggcttt gcgctctaac gagtcagcta accggcgcgc
     1021 ggctaatccg cgtccgaatt aagcaatcgg taaagcgaaa cgtgtagcac aagaatatca
     1081 atcctctcgg aagccgctta gtctgggacg atctcgcatt ctgagatggc aagtttacaa
     1141 aaccctgcct cagctagaat gatctgggag atatgcatta gtaccaaagg gctttgaaat
     1201 ttctctccac ggtgatcgtc tgcaagcggt aaacgcatca ggtcgtatag aaaagacgca
     1261 attccaaacg tgagcgggtt ttcgtttcgt tcaagggcct catcccagtt tttctgataa
     1321 tggttccata actattcaat aaaggcttcc tcaagagcgg ggctaaatct cttcatgaca
     1381 agataacgga tacggaggaa ttggtcagga aatagggcgt ttttcaatgc ctgcatctct
     1441 gcgaaaagga tgcacgtgat aaggaagggc gcataggctt caggcagatc gggagaggcg
     1501 ctcccggtag ccaagtggtt tagcgattga cggaggccct gtcgaatcga ttcccaattc
     1561 tcgtatatat ccttcgaaac cttgtggact aacgagtatg caagctccct ctccgtcagt
     1621 ttctttttcc cgaattgtcc catagtgcgc tgcctaacgc cctcgctcag cggcgcgctg
     1681 cccacgctcc agaaaactcg ccagagcttt ctcgcgtctg ctgcagcgag atgttatagc
     1741 tagatccacg atggatcggt cgtgctgatc cttgcctcgc gtgagaaatt tctcacctct
     1801 ccgaaaagca cctcgattgc cgcagcaagt gtcatatgcc ttcgagccgc cgtcgctcca
     1861 cgcgatacta ccagcccttg cttttcaaat ttgtgaacga tcttcgacac ctcgtctcgc
     1921 gacaacttcg tagcggcagc tatctgtact ctggtgcggg gccgccgcat tgcttccaat
     1981 actcgttctt cgtctctggt catagctcct ccgatcacgc ctgctaggga tcagtcccgg
     2041 ccaatatagc tctaactaca aatagactgc cgaaaagcgg tatatcacgg ctgcatattt
     2101 gggcagattt aggtagaaag ggctttagcg ttgcatgctt agctttaatt aaaaatacta
     2161 acaaaacccg tatttgtaag gttcgtcttt tggctcccgt tggagtaaca ctggctacat
     2221 ctgcaagtat acgacggctc gtgagctgag ggtaagcgcc tatgccaggg taattggtgg
     2281 ctggtatgtt ttaaatcccc cttctgagcc gccaagtgac gaggtcaaat cgggggcttt
     2341 caagcgagca ctgtttgagc gcgaagcgcg agttgcgcag ccgcccaatt tgccaacaaa
     2401 ccgaaggaat ccgcaggacg gcgcagcagg gtgccctttt ctttggtttc tttcttttgg
     2461 gcaagcaaaa gaaagaaacc agccgccggg ctgcccccgg catcttatta catcaataca
     2521 atatcgtact gttcctgcgt gtactgcgtt tccacctgaa gcgagaccgg cttgccgatg
     2581 aaatcgccga gcatggccag gctttgcgac tccttgtcga ggaacagatc aattacatcc
     2641 tgtgaagcga gaatgcggta ctcgcgcgta ttgaactgcc gcgcttcacg cagcagctcg
     2701 cgcaggattt cgtagcacac ggtctgcgcg gtcttcactt cgccgcgccc ctcgcaggtc
     2761 gggcacggct cgcacagcac atgggcgagg ctctcccgcg tgcgcttcct cgtcatctcc
     2821 accaaaccca gggcggtgaa gccgttcacc gtcatgcgcg tgcggtcacg cgccagcgcc
     2881 ttcctgaatt ccgccagcac cgcgttgcga tgctcttcgc tgtccatgtc gatgaagtca
     2941 atgatgatga tgccacccag atttctgagt ctcagttgcc gtgcaatcac atgcgccgct
     3001 tcgagattgg tcttgaagat ggtgtcgtcg aaattgcgcc cgccgacaaa gccgccggtg
     3061 ttcacatcca cggtggtgag cgcctcggtc tggtcgataa tcagataacc gccggatttc
     3121 agctcgacgc gccgcgccag cgctttctgg atttcctctt ccacgccgtg aaggtcaaac
     3181 agcgggcgct cgccggtata gtgttcgatg cgcggcacca tgttttccat gtaattctgt
     3241 gcaaacgact gcattttgtg aaaagtctcg cgcgaatcca ccaccacccg ttcgctatct
     3301 tcgctcacga aatcacgcaa tacgcgcaag ctcagggtga ggtcttgata gagcagcgtc
     3361 ctcggcggcg ccgattgcgc ttgttcccga atcccgctcc acagcttgcg caagtactcg
     3421 acgtcggcga gcagctcgcg ctcgcttgcg gtttcggcca tggtgcgaat gatgaagccg
     3481 ccgctttggt cctgcggcag aagctgctgc agcttgtcgc gcagatgggc gcgctcttcc
     3541 tcgtcctcga tgcgctgcga aatgccgatg tgcgtttcct gcggcaggta taccagcatg
     3601 cgcccggccg cgctgatttg cgtggaaagc cgcgccccct tgctgccgat gggatcttta
     3661 atcacctgca ctaaaagctt ctgcccctcg tgcagcaatc tttcgatggg cttcggctct
     3721 tcgccgtttt gccattgccc ccagatgtcc gccacatgca ggaacgccgc acggtcgagg
     3781 ccgatttcaa caaacgccga ctgcatgccg ggcagcacgc gacagacctc gccgaagtaa
     3841 atgttgccga ccaagccgcg gccgctcacc cgctcgatgt gcagctcctg cactacgccc
     3901 tgccccatca ccgcaacgcg cgtctcctgg ggggtaacgt taattagaat gtcttcgctc
     3961 acagcacctg gtaaccgaat tcccgcatta attctgccgt ctcgaacaac ggcagaccca
     4021 tgatgcccga gtagcttccg tcaatttccg caataaatgc cgcggccaaa ccttggatcg
     4081 cgtagccgcc cgccttgtcg agcggctcgc cggtggcgac gtaattcaga atctcctggt
     4141 ccttgagctc gcgaaaacgc accgatgtca cggacaggcg cagttccagc ctgtcctcca
     4201 gcttcatcgc caccgcggtc agcacttgat gaatttgccc tgagagttcc ttcaacatcc
     4261 gcaccgcatg ttcgcgattt tccggcttgc cgatgatttt tccgcccagc gccactgtgg
     4321 tgtccgccgc cagcacgggg aagtgccgca gccttcttcg aatgattgac gaccagccgg
     4381 cttcggcttt ggcgcacacg atgcgcttca cataatctcc cggaggctcg ccgggatggg
     4441 gcgattcgtc cacgtcggca ccgcgcacaa ggtattcccg aaacagcacg acctcgaaat
     4501 tcacgtcgat ctgttttaac agctcgcgcc ttctggggct cctcgaagcg aggtataggt
     4561 gctggtcggg acgtttcatt ttgttgatta ccggcgatga taagggtgat tatgcagcaa
     4621 cgacaccgcg cggtagagct gttcggccag caccacgcgt gccagcgcgt gcggcagcgt
     4681 catggcggaa agcccgagca gcatgtcggc ggaagtcttg atttcattat ccaggccgtc
     4741 ggcgccgccg aggacaaacc ccacatcgcg ccccgcctgc atccagcgcg caagactttg
     4801 ggccagctgc gccgtgctca gcgccttgcc cttttcatcg agcaccacgc gccggcagtg
     4861 gcgtggcagg gcggcaagaa tgcggccctt ttccgcttcc agaacttgct ggatgttttt
     4921 ccccgctcca cgtttttccg gcttgatttc gatgagctgc acccgcgctt cctgcggcat
     4981 gcgctttgcg tattcggaaa aacccgtccc cacccaggcc ggcattttat cgcccaccgc
     5041 aatgatccaa agtttcatcg ccgcctggcc taaaccgcgg cggctttgcg acggcgccgc
     5101 acgggcttat gctcaccacc ccacaattgc tcgagattgt aataagtgcg aaccgcgggt
     5161 tgcatgatgt gcaccaccac attgccgaag tccaccagca cccaatcgcc gttctcctct
     5221 ccttccacgc cgtaaacttt ggcgcctagc gccttgagtt tctcctgcac attgctggaa
     5281 agcgccttgc tctgccggcc cgaatcggcg ctggcgataa tcatgcggtc gcacagccaa
     5341 gtcatcttgc gcacatccag caccatgata tcgcgcgcct tgatgtcttc cagcgccgca
     5401 accgtggctt tcaccattct atcgattctc atccgcccct agtatataaa tgattaatcc
     5461 gaatataatc gagaaccgcc gggggaagca aatcgcgcac agtagcaccc gcggccagtt
     5521 gttcccgaat tatggaggcc gatatatcca ctggcgtgat ttccagaacc acgatactgc
     5581 cgcaggggct gctgactgcg gtgcgcggcg acggagcacg gcgtttggcg aattccgcag
     5641 cgagcaccgg cggcaaatcc ttgagcgaga atcccggccg cagcgcgatg gcgacatggg
     5701 caaggttaaa cagctcttcc cagcgccgcc aggtcgcaag cccggcaaag gcatcggttc
     5761 caagcagcaa acacagcgga cgtgaagcac ccacctcttg gcgcagctca ttcagggtat
     5821 cgacggtgta gctggcgcgc tcgcgcgaga tttcccgctg atccacggaa aatcgcgggt
     5881 ttccggaaat cgccaattgc agcatgtcca cacgcgcttt agatgatgca aaaggggcgg
     5941 ggcgatgcgg cggcgtgccg gccgggatga aacgcacttc ggcaagattg agttgttcgg
     6001 cgacttcagc ggcaagtgtg agatggccgt aatgcagcgg gtcaaacgtg ccgcccaaaa
     6061 tgccgatagg gtttaacgcg ttcactacag cagcaagcgg cggatgtcgg aaagtaccga
     6121 gcagagataa gtggtgaaac gagcggcggc ggcgccgtca atcacgcgat gatcgtagga
     6181 aagagagagc ggcagcagca gtcgcggcac caactggcca ttttggtaca ccggcttcaa
     6241 ctgcgccttg gaaacaccca gtatcgctac ctcgggcgcg ttgatgatgg gcgtaaacgc
     6301 cgtgccgccg aggccgccca ggctcgaaat cgaaaaacat ccgccctgca tttctgcggg
     6361 cgagagtttt ttatcccgcg cccgcttgct gatttcggcg agctggcgcg ccagatcaaa
     6421 cacgcccttg cgatcggcgt cccgtatcac cggcactacc aggccgtcgg gcgtgtccac
     6481 cgccacaccg atatggtaat actttttgag cacgaggttc tcgccgcttt tgtccagcga
     6541 tgcgttgaat tccggatgta ctttcagcgc ggtcacgcaa gccttgatta aaaacgacag
     6601 catggtgagc ttgaagccct gcttttcggt ttccgcgctg ttcgacttgc ggaaagcttc
     6661 aaggtcggtg atgtcagctt catcgtgctg cgtgacatgc ggaatgctta cccagttgcg
     6721 atgaagattg gcaccggaaa gcttcttgat gcgtgacagc ggcttggatt cgatggggcc
     6781 atacttggcg aaatccacct gcggcatttc cggaagcgca aaacctgcgc cgccacgcgg
     6841 ccgcgacagc tcgaccttta cataggcctg cacatcttcc ttgaggatgc ggctctttgg
     6901 cccggaaccg ccaacttgat cgagattaac acccatctcg cgcgcgaagc ggcgcaccga
     6961 ggggctagcg tgaggaaggt gcgtacgttc cggca
//
LOCUS       DATIOW010000195         1522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1103380, whole genome shotgun sequence.
ACCESSION   DATIOW010000195 DATIOW010000000
VERSION     DATIOW010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103380"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VLL03_06490"
     CDS             <1..322
                     /locus_tag="VLL03_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HSS47042.1"
                     /translation="RAQYTPLRAPTDPAGRVVIVVDDGLATGSTMIAALRALRQRNPE
                     KLICAVPVAPPDTLEKVTHYADQVVCLHASVDFFAVSQFYGEFPQVEDEEVMSILRQT
                     GGSN"
     gene            387..1082
                     /gene="ispD"
                     /locus_tag="VLL03_06495"
     CDS             387..1082
                     /gene="ispD"
                     /locus_tag="VLL03_06495"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HSS47043.1"
                     /translation="MNHFALIPAAGTGVRLGGQTPKQYLPLNGKPMLFYSVRQLCANP
                     RIKRVYVVLAHGDEWFSQYDWKAFAAKLAPLYCGGETRAASVLNGLLAATGEIGADNW
                     VLVHDAARPCLSDVLLNKLLDEVADDGVGGLLALPVADTLKRADGAERVLRTEPREGL
                     WQAQTPQMFRHETLTLALQAMQNREVTDEARAIEGFGLQPKLVVSDARNLKVTHAQDL
                     PLAELILKSMEKA"
     gene            1079..>1522
                     /gene="ispF"
                     /locus_tag="VLL03_06500"
     CDS             1079..>1522
                     /gene="ispF"
                     /locus_tag="VLL03_06500"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HSS47044.1"
                     /translation="MRVGQGFDVHALVENRKLIIGGVEIPYKKGLAGHSDADVLLHAI
                     CDALIGAAALGDIGAHFPDTDPRYKGIDSRKLLREVGKLLTGNGWRVINIDATIIAET
                     PKMAPHIPAMVKNIAQDLAIDVKDVNVKAKTNEKIGSIGRGEGIIV"
BASE COUNT          348 a          406 c          472 g          296 t
ORIGIN      
        1 ccgcgcgcaa tacacgccgc tgcgcgcgcc gacagacccc gcagggcgcg ttgtcatcgt
       61 cgtggatgac gggcttgcca ccggttccac catgattgcg gcgctgcgcg ctttgcgcca
      121 aagaaatccc gagaaactga tatgcgccgt gccggtggcg ccgccggaca cattggagaa
      181 agtaactcat tatgccgatc aggtagtgtg cctgcacgct tccgtagatt tctttgcggt
      241 gagccagttt tacggagaat tcccgcaagt ggaagacgag gaagtgatgt ctatactccg
      301 ccaaaccggc ggctcgaatt aaaaacgcat tgccggcaag ctacggaaaa agtgataatg
      361 caaattctat tacaaggcct ggaatcttga accacttcgc tttgatcccg gccgccggca
      421 ccggcgtgcg cttgggcggg caaaccccca agcaatatct gccgcttaac ggcaaaccca
      481 tgctgtttta ttcggtgcgg cagctgtgcg cgaacccgag gatcaagcgc gtctatgtag
      541 tcctggcgca cggtgacgaa tggttttcgc aatacgattg gaaagccttt gcggcaaagc
      601 tggcgccttt atactgcggt ggggagacgc gcgcggcgag cgtgctgaac ggactgctcg
      661 ccgcaacagg tgaaatcggc gcagataatt gggtgctggt gcacgatgcg gcgcggcctt
      721 gtttgagcga tgtgttgctc aacaagctgc tggacgaagt ggctgacgac ggtgtgggcg
      781 gtttgctggc gctgccggtc gcggatacgc tcaaacgcgc agacggcgcg gagcgggtgc
      841 tgcgcaccga gcccagggag gggctgtggc aggcgcagac gccgcagatg ttccgtcacg
      901 agacgctgac acttgcgttg caggcgatgc aaaacaggga agtgaccgat gaggcgcgtg
      961 ccattgaagg attcggtttg caaccgaaac tggtggtgag cgatgcgcgc aatctcaaag
     1021 tgacccacgc gcaggatttg ccgctcgccg aattgattct gaaaagcatg gagaaagcat
     1081 gagagtcggc caaggatttg atgtccacgc actagttgag aaccgcaagc tgatcatcgg
     1141 cggggttgaa ataccttata aaaaaggcct cgcgggacac tccgacgcag atgtgctgct
     1201 gcacgcgatt tgcgatgcgc tgatcggcgc cgccgctttg ggtgatatcg gcgcgcattt
     1261 tcccgacacc gacccgcgct ataaaggcat agacagccgc aaactcctgc gcgaagtggg
     1321 taagctttta accgggaacg gctggcgcgt catcaatata gatgccacca ttattgccga
     1381 aacgccgaaa atggcgccgc acattcctgc catggtgaaa aacatcgcgc aggatcttgc
     1441 cattgatgtc aaggatgtca atgtcaaagc caagaccaac gagaaaatag gttctatcgg
     1501 gcgcggcgaa ggcatcatag tg
//
LOCUS       DATIOW010000196         2723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1001152, whole genome shotgun sequence.
ACCESSION   DATIOW010000196 DATIOW010000000
VERSION     DATIOW010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001152"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1412)
                     /locus_tag="VLL03_06505"
     CDS             complement(<1..1412)
                     /locus_tag="VLL03_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HSS47045.1"
                     /translation="MRIDKFTTKFQQALSDAQSIAVGHDNPYIEPQHLLLALLDQEDG
                     GTASLLGRAGVNVPPLRAALKKSIDALSKVEGNAGEVSVSRELASLLNLTDKEAQKRG
                     DQFIASELFLLALTQDKGETGRLFKQHGADRKHLEQAISAVRGGENVVSSEAEGSREA
                     LKKYTLDITERARMGKLDPVIGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPETLKNKSVLALDLAALLAGTKYRGEFEERLKSVLKEIAKEEGRIIVF
                     IDELHTMVGAGRAEGAIDAGNMLKPALARGELHCVGATTLDEYRKYVEKDAALERRFQ
                     KVMVKEPTVEDTIAILRGLQEKYELHHGVEITDPAIVAAAELSHRYITDRFLPDKAID
                     LMDEAAARIKMEIDSKPEAMDKLERRLIQLKIEREAVKKEKDEASKKRLSLIEGEIKK
                     LERDYADLEEVWKAEKAQVQG"
     gene            1560..1982
                     /locus_tag="VLL03_06510"
     CDS             1560..1982
                     /locus_tag="VLL03_06510"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSS47046.1"
                     /translation="MAKVQMETKLVVPANILWDAIGRFSALGDWHPAVEKCVSDGESK
                     GSKRKLSLVGGGSIIERLEHISDKEKLYRYSITDSPLPVADYNAVIRVKDNGDGTSTV
                     EWSSNFEPKGAKADEAVKIIQGIYQAGFDNLKKMYPKK"
     gene            2027..2227
                     /locus_tag="VLL03_06515"
     CDS             2027..2227
                     /locus_tag="VLL03_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS47047.1"
                     /translation="MSERRAKPRLSPLGRDITLMLVIKAIALYLIWLAWFSSPQDKNI
                     DTDQIGKTLITSPVKDSNHAQR"
     gene            2214..>2723
                     /locus_tag="VLL03_06520"
     CDS             2214..>2723
                     /locus_tag="VLL03_06520"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004540940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HSS47048.1"
                     /translation="MPSAEVVELSRLQFAITALYHFLFVPLTLGLSWILVIMESVYVM
                     TGKQIYRDMTQFWGKLFGINFAMGVATGITLEFQFGTNWSYYSHYAGDIFGAPVALEG
                     LMAFFLESTFVGLFFFGWQRLNKVQHLAVTFLVALGSNLSALWILIANAWMQNPVGAE
                     FNYETMRMEL"
BASE COUNT          546 a          797 c          732 g          648 t
ORIGIN      
        1 ccctggacct gggctttttc cgctttccag acttcttcca gatcggcata atccctttca
       61 agtttcttga tttccccttc aataagagac aagcgttttt tcgaggcctc gtctttttct
      121 ttcttcaccg cttcgcgctc gatcttcaac tggatcagcc gccgctccag cttatccatc
      181 gcctccggct tggaatcaat ttccatcttg atgcgcgccg ccgcttcgtc catcaagtca
      241 atcgccttgt cgggaagaaa acggtcggtg atgtaacggt gcgaaagttc cgccgcggcg
      301 actatcgccg ggtcggtgat ttcgacgccg tggtgcaatt catatttttc ctgcagcccg
      361 cgcaaaatgg cgatggtgtc ttccaccgtc ggttctttca ccatgacttt ctggaagcgc
      421 cgctccagcg ctgcatcttt ctcgacgtat ttgcggtatt cgtcgagcgt ggttgcgccc
      481 acgcaatgca gctcgccgcg cgccagcgcc ggtttcagca tgttgccggc atcaattgcg
      541 ccctccgccc tgccggcgcc caccatggtg tgaagctcgt ctatgaaaac gatgatgcgg
      601 ccttcttcct tggcgatttc cttgagcacc gatttcagcc gctcttcgaa ttcaccgcgg
      661 tatttcgtgc ctgccagaag cgccgccagg tcgagcgcaa gcacgctctt gtttttcagc
      721 gtctcgggca cttcgccgtt gacgatgcgc tgcgccaggc cctcgacaat cgcagttttg
      781 cccacgcccg gctcgccgat taataccgga ttgttcttgg tgcgccgctg cagcacctga
      841 atagtgcggc ggatttcgtc gtcgcgcccg atgacgggat cgagcttgcc catacgcgcc
      901 cgctcggtga tatccagcgt gtattttttc aatgcttcgc ggctcccttc cgcttcggaa
      961 ctgaccacat tctctccgcc gcgcaccgcg ctgatcgcct gctccagatg cttgcggtcc
     1021 gcgccatgct gcttgaacaa cctgccggtc tcgcccttat cctgcgtcaa cgccagaagg
     1081 aaaagttcgg atgcgatgaa ctgatcgccg cgtttctgcg cttccttgtc ggtgaggttc
     1141 agcaggctcg ccaactcacg cgagacgctg acctcgccgg cattgccttc gaccttggat
     1201 aaagcatcta tgcttttctt gagcgctgcg cgcagcggcg gcacgttgac tcccgcacgg
     1261 ccgagaagcg atgcagtgcc gccatcctcc tggtcaagca acgccagcag caagtgctgc
     1321 ggctcgatgt aaggattgtc gtggccgacg gcgatgctct gcgcatcgga gagcgcctgc
     1381 tggaacttgg tggtaaattt gtcaatgcgc ataaaaactc cgggaattgg aaccggttat
     1441 cggaactata aatggggctt tagcgcgctg tttcaagccg ccgcgtaatt aagatttggc
     1501 ctgctgtgcg gttttggagc aaaatagacc atccgcaggc aatacacaag gagcacatca
     1561 tggcaaaggt ccaaatggaa accaagctcg tggttccggc aaatattttg tgggatgcca
     1621 taggccgctt cagcgcgctg ggggactggc accccgcggt ggaaaaatgc gtatcggacg
     1681 gcgaaagcaa gggttcgaag cgcaagctga gcctggtcgg cggcggcagc atcatcgaac
     1741 ggctggaaca catcagcgac aaggaaaagc tctaccgcta ttcgattact gatagcccgc
     1801 ttccggttgc cgactacaac gccgtaatca gggtaaagga caacggcgac ggcacctcga
     1861 ccgtcgagtg gtccagcaac tttgaaccaa aaggcgcaaa agcggacgag gcggtaaaaa
     1921 tcattcaggg catttatcaa gcgggcttcg acaatttgaa aaagatgtat cccaagaaat
     1981 aactgaacca ggccttgccc ccgatttcat tgaaattaat ccggacatga gcgaacgacg
     2041 agccaagccg cgcctctccc cacttgggcg cgacatcacg ttgatgctgg ttatcaaggc
     2101 gatcgcgctt tatctcatct ggctcgcctg gttttccagc ccgcaagaca aaaatatcga
     2161 tactgaccag atcgggaaaa ccttgattac cagccccgtc aaggattcaa accatgccca
     2221 gcgctgaagt cgtcgaatta tcgcggctgc agttcgcgat caccgcgctt taccatttcc
     2281 tgtttgtgcc gttgacgctg ggattatcgt ggattttggt catcatggag tccgtctacg
     2341 taatgaccgg caagcagatt tatcgcgaca tgacccagtt ctggggcaag ctgttcggca
     2401 tcaatttcgc gatgggggtc gcgaccggca tcacgctgga attccagttc ggcaccaact
     2461 ggtcgtatta ctcgcattac gccggtgata ttttcggcgc gccggtggcg cttgagggac
     2521 tgatggcgtt cttcctcgaa tccacttttg tcggcctgtt tttcttcggc tggcagcggc
     2581 tgaacaaagt gcagcatctt gccgtcactt tcctggtagc gctgggctcc aatctctcgg
     2641 cgctgtggat actgatcgcc aatgcctgga tgcaaaaccc ggtgggcgcg gaattcaatt
     2701 atgaaaccat gcgcatggaa ttg
//
LOCUS       DATIOW010000197         4490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_390541, whole genome shotgun sequence.
ACCESSION   DATIOW010000197 DATIOW010000000
VERSION     DATIOW010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390541"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VLL03_06525"
     CDS             complement(<1..646)
                     /locus_tag="VLL03_06525"
                     /inference="COORDINATES: protein motif:HMM:NF016367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HSS47049.1"
                     /translation="MTQFLVSVKSLDEARIALECGADIIDLKDPANGALGALPPGIVR
                     ETVKWVNRRCPVSATVGDLPMEPVALVHAVKEAAAGVDYVKVGFFPGGDSLACIHALA
                     RHAGQARIVAVLFADCHPDFSLLVDLAQAGFAGVMLDTAEKSGGGLRNYCSAPRIAEF
                     IQSARAVGLMTGLAGSLRLTDVPDLIKLEPDYLGFRGALCAGSKRNRVMQDSRVS"
     gene            complement(643..1218)
                     /locus_tag="VLL03_06530"
     CDS             complement(643..1218)
                     /locus_tag="VLL03_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 domain-containing protein"
                     /protein_id="HSS47050.1"
                     /translation="MIFETIITTQASDGRIHIAPMGIREDNGLIVLAPFRPSTTLDNV
                     LGNSHAAVNYCDDVRIFAGCLTGRRDWPTVPADKVPCVRLESALTHCELKLERIEEDE
                     LRPRLLCQVVHRVSHSPFRGFNRAQAAVLEAAILVSRLHLLPREKIDAEVKYLAIAID
                     KTAGPREREAWSWLMEKIEAHRASPKQGSTA"
     gene            complement(1338..2744)
                     /locus_tag="VLL03_06535"
     CDS             complement(1338..2744)
                     /locus_tag="VLL03_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6513 domain-containing protein"
                     /protein_id="HSS47051.1"
                     /translation="MPERILFLTGHLAEKSLNKILAAMRPSEFEYDVLNIGISVAGLM
                     TADLVRRRLPGPGQATRIIVPGRCRGDLDVLSRHYGVPVVRGPDELKDLPQFFGKKGA
                     SEDLSHYDVRIFAEIVDAPQSSIEQILRRAESYRKDGADVIDLGCLPETPFPHLEEAV
                     TALKLAGYQISVDSVEPKELLRGGKAGADYLLSLKEDTLWVAEEVDSIPVLIPQLPGD
                     LASLERAMEKMAKRNRRFVADSVLDPIHFGFTDSLARYHELRRRHPEAEIMMGVGNVT
                     ELTDADTTGINALLMGIISELRITNILTTEVSPHARRAVREADVARRMLYAAREASSL
                     PRDFSSELLTTHARKPFPDTVEEIAELARAIKDPSYRIQISEQGIHIYNRDGVHVAAD
                     PFALFPLLNLENDAAHAFYLGVELARAQIAWQLGKRYNQDEELDWGCAVERKPKDLTQ
                     HHAPGTTLEVVKKKAKNK"
     gene            complement(2779..3330)
                     /locus_tag="VLL03_06540"
     CDS             complement(2779..3330)
                     /locus_tag="VLL03_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HSS47052.1"
                     /translation="MHDHKPRLAWALTGSGHFLKESLEIALELPHVDLYLSKAAAEVV
                     HMYAHSMKELRARLRVFRDTTASAAPVGLFYYGHYHTVVIAPATSNTVAKCVLGISDS
                     LVTNIFAQAGKCRIPSIVFACDTEPVVTTEAPKEWVTLYPRKVDLDNTEKLKNFENVT
                     VVMRIEELREAVDARMKTLGIKI"
     gene            complement(3737..>4490)
                     /locus_tag="VLL03_06545"
     CDS             complement(3737..>4490)
                     /locus_tag="VLL03_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007742202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSS47053.1"
                     /translation="IESELFGYKAGAFTGASREGQRGKIFQANGGTLFLDEIGDMPIQ
                     LQARLLRVLEEREVLPLGSEIPIKVDIKLISATHCNLTEKIVKGEFRQDLYYRLNGLS
                     VSLPPLRERVDKSNLIKHILAAENTDKHKVEVDDQALEALERYYWPGNIRQLRNVLRT
                     VLALREDDVITIKDLPQEIGGGRAPLHENNNSAATLNPLESAERDALTRELESHHWNI
                     TNVARQLKISRNTLYRKMQRLNIKDPNKDILD"
BASE COUNT          877 a         1301 c         1314 g          998 t
ORIGIN      
        1 cggagacccg tgaatcctgc ataacccggt ttcttttcga gcccgcgcac agggcgccgc
       61 ggaaccccag gtagtcgggt tcaagtttga tcaggtcagg aacatccgtg agccgcagcg
      121 atccggccaa tccggtcata agtcccacgg cacgggccga ctgaatgaat tccgcgatac
      181 gcggtgcgct gcaatagttt cgcaagccgc cgcctgattt ctctgcagta tccagcatca
      241 cgccggcaaa acccgcttgc gccaaatcta cgagaaggga gaaatccgga tggcagtctg
      301 cgaacagcac tgcaacgatg cgtgcctgcc cggcatggcg cgccagtgca tggatacaag
      361 cgagggaatc gccgcccggg aaaaatccga ccttcacata atccacgccc gccgccgctt
      421 ccttgactgc atgcacaagc gctacgggtt ccatcggcaa gtcgcccacg gtggcgctta
      481 ccgggcagcg gcgattgacc catttcaccg tttcccggac gatgccggga ggcagcgccc
      541 ccagcgcgcc gtttgccggg tctttgagat cgataatatc ggcgccgcac tccaaggcaa
      601 tgcgcgcttc atccaacgat ttcacgctca ccaggaattg cgtcatgcgg tgctgccctg
      661 cttgggtgac gctcgatggg cttcgatttt ttccatgagc cagctccacg cctcgcgctc
      721 gcggggccct gcggtcttgt cgatggcaat cgcgaggtat ttcacttcgg catcgatttt
      781 ttcccggggc agcagatgca ggcggctcac cagaatcgcc gcctcgagca ccgccgcctg
      841 cgcgcggttg aagccgcgga acggcgaatg ggagacacga tgtaccactt ggcacaaaag
      901 ccgagggcgc aattcgtctt cttcgatacg ttcaagtttc agttcacaat gcgtaagcgc
      961 tgactcaagc cgcacgcagg gtactttgtc agcgggcacg gtgggccagt cgcggcggcc
     1021 ggtgagacag cctgcgaata tgcgcacgtc gtcgcaataa ttgactgctg cgtggctgtt
     1081 tccgagcaca ttatccagcg tggttgaggg acggaacggc gcgagcacga tgagaccgtt
     1141 gtcttcgcgg atgcccattg gcgcgatgtg aatgcggccg tcactggcct gggtggtgat
     1201 gatggtttcg aaaatcattt gatccttgaa catctctaac cggggaaaca gtaaatcaat
     1261 ttagacaaga tttacaggat tttgcaagat caacaggatt aaatcatgtc aattctgaaa
     1321 aatcctgtta atcctgtcta tttatttttt gcctttttct tcaccacttc aagcgtagtt
     1381 ccgggtgcgt ggtgctgcgt caggtccttg ggtttacgct ccaccgcgca gccccagtcg
     1441 agttcttcat cctgattgta gcgcttgccc aattgccagg cgatttgcgc gcgcgccagc
     1501 tccacgccga gataaaatgc atgcgcggca tcattttcca gattgagcag gggaaacagg
     1561 gcaaacggat cggcggcgac atgcactccg tcgcggttgt agatatggat gccctgttcg
     1621 ctgatctgga tgcggtagct cgggtccttt atggcgcggg ccaattcggc gatttcttcc
     1681 acagtgtcgg gaaagggttt gcgcgcatga gtggttagca gttccgaaga aaaatcgcga
     1741 ggcaggctcg acgcttcgcg cgcggcatac agcatgcgcc gcgccacatc cgcttcgcgc
     1801 accgcgcgcc gcgcgtgcgg actcacttcg gtggtaagga tattggtaat gcgcaattcg
     1861 gaaatgatac ccatcagtag cgcgttgatg cccgtggtgt cggcgtcggt gagttcggtg
     1921 acattgccca ctcccatcat gatttccgct tccgggtggc ggcggcgcaa ttcatgatag
     1981 cgcgccagcg aatcggtgaa gccaaaatga atcgggtcga gtaccgaatc ggcaacgaaa
     2041 cggcggttgc gcttcgccat tttttccatc gcgcgttcca gcgaagcgag atcgccaggc
     2101 agctgaggaa tcaacaccgg gatcgagtca acttcctcgg ctacccataa ggtgtcttct
     2161 ttcaggctta acagataatc ggcacctgcc ttgccgccgc gcagcagctc ctttggttcc
     2221 accgaatcca cgctaatttg atatcccgct aacttgagcg cggtgactgc ttcttcgagg
     2281 tggggaaacg gcgtttcggg cagacagccc aggtctatca catctgcgcc gtccttgcga
     2341 tagctttccg cgcgccgcag gatttgttcg atgcttgact gcggtgcgtc aacgatttca
     2401 gcgaaaatgc gcacgtcgta atggctgaga tcttcgcttg ctcctttctt tccaaagaac
     2461 tgcggcaggt ctttcaattc gtccggtccg cgcaccaccg gcacgccgta gtggcgggaa
     2521 agcacatcga ggtcgccgcg gcaacggccg ggcactataa tgcgcgtcgc ctgtcccggc
     2581 ccgggcagtc gccggcgaac taggtcggcg gtcatcaggc cggcgacgct gatgccgata
     2641 ttgagcacat catattcgaa ctcgctcggg cgcattgcgg cgagtatctt gttgaggctt
     2701 ttttcggcaa ggtggccggt gagaaataaa atccgttcgg gcatttgctt aataatatac
     2761 cccagtagga ctggcagttc agattttgat gccgagtgtt ttcatccgcg cgtcgaccgc
     2821 ctcgcgcagc tcctcgatgc gcatcaccac ggtgacgttt tcgaagtttt tcagcttttc
     2881 cgtgttgtcg aggtcgacct tacgcggata aagcgtcacc cattccttcg gtgcttctgt
     2941 ggtcactaca ggctcggtat cacaagcgaa gacgatactg ggaatgcggc acttgccggc
     3001 ttgcgcgaag atgttggtga ccaggctgtc ggaaataccc aacacgcact tggcaacggt
     3061 gttggaagtg gcgggggcga tcaccacagt gtggtagtga ccgtaataaa acaatcccac
     3121 cggcgcagcg ctggcggtgg tgtcgcggaa cacgcgtaag cgggcgcgca gttccttcat
     3181 actgtgggcg tacatgtgca ccacttcagc cgccgccttg gacaaataca ggtcaacgtg
     3241 cggaagctcg agcgcgatct ccagcgattc ctttaggaaa tgtccggagc cggttagcgc
     3301 ccaggcgagg cggggcttgt ggtcatgcaa cttagaacct atctcaacaa tacccacatg
     3361 atgcgttgct ggccagaaag cgatgactgc aaggagcgag gaggccgcat agccccacta
     3421 tgcaacgacg agcaacgcag caggcgcgct ttcgtggccg caaccctgct ggacgggggc
     3481 cgtcgcgacc cggtggcgaa gccgggtgcg ggagcgacga gcagtgcgcc caagcagggt
     3541 tggttttcga tggcctttgc gccccttccc gcgccacgct gcgcgagacc gagtccgggg
     3601 cgcactttgc gccatttcgg cgttgctcgc cgcttatttg gaacaaacca aaccgcgcgg
     3661 ctcgcgccta gaactgtcac caaaatcgcc tccgtcgcac acgtgagtat tgttgagata
     3721 ggttccaacc caccccctag tcgagaatat ctttgtttgg gtccttgata ttcagccgct
     3781 gcatcttgcg gtacagggta ttgcggctga ttttgagctg acgcgccacg ttggtgatgt
     3841 tccagtggtg gctttccaat tcgcgggtca gcgcgtcgcg ctccgccgat tccagagggt
     3901 tgagtgttgc cgcggagtta ttgttttcgt gcaatggcgc ccggccgccg ccgatttcct
     3961 gcggcaggtc cttgatggtg atgacatcat cctcgcgcag cgccagcacg gtgcgcagca
     4021 cgttgcgcaa ctggcggata ttacccggcc agtaatagcg ttccagcgcc tctagcgcct
     4081 gatcatcaac ctcgacttta tgcttgtcgg tattttccgc tgccagaatg tgcttgatca
     4141 agttgctttt gtcgacgcgc tcgcgcagcg gcggcaatga cacgctgagc ccgttcaggc
     4201 ggtaatagag atcctggcgg aactctccct tgacaatttt ttccgttagg ttgcagtgtg
     4261 tggcgctgat cagtttgata tccactttaa tcggaatctc gctgcccagt ggtaggacct
     4321 cgcgttcctc gagcacgcgc aacagccgcg cctgtagttg gatcggcatg tcgccgattt
     4381 cgtccaggaa cagcgtgccg ccgttggcct ggaaaatctt gccgcgctgg ccttcgcgac
     4441 tggctccagt gaacgcgccg gccttatagc caaacaattc ggactcgatc
//
LOCUS       DATIOW010000198         2072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_390809, whole genome shotgun sequence.
ACCESSION   DATIOW010000198 DATIOW010000000
VERSION     DATIOW010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390809"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..1421
                     /locus_tag="VLL03_06550"
     CDS             48..1421
                     /locus_tag="VLL03_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HSS47054.1"
                     /translation="MKDKHNQAAGETPQLQGVTRRRFIKATGAAGLAASVAPFIIVPP
                     ARAAKKTLKLLQWNHFVPGYDKWFDNTYTKEWGAKNDTEVIVDHVGIQALSARAAAEV
                     GAQKGHDLLMFLSPPPAYEDQVIDHNEVYQECERKHGKPVPLAVRSTYNPKTKKYFAF
                     SDSYVPDPVNWRKDLYDAVGAAAPSTWDDVRVAGRKIKEKFNIPVGIGLSSELDTAMA
                     MRAALYAFGGSIQDEKANVVLNSKNTLEAVKFVKALYQETMTPEVLAWDPSSNNRAML
                     AGQISVALNAISITREGENKNIPGTDNIWLAKALAGPVRRIGMEHVMDCYVIWKFAEN
                     IEGAKKFLTDYIDNFRLGFLASEFYNFPSFPSTVSDIDKLLANDTKAHPSDKYKVLAD
                     VLDWATNVGYPGYANAAEDEIFNTWVINTMFAKAATDTETPENAIKAAEEQCKRIFAK
                     WREKGLV"
     gene            1498..>2072
                     /locus_tag="VLL03_06555"
     CDS             1498..>2072
                     /locus_tag="VLL03_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011514944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSS47055.1"
                     /translation="MATVEARSITKSFDGSNAVDGINLLVKEGEFLVLLGPSGSGKST
                     LLRMIAGLEVPTAGDILIGGKIVTLFPPKARNIAMVFQSYALYPHLSVFKNIAFPLEA
                     QGMKSEAVKNKVEHAAAMFGIGRLLARKPRQLSGGERQRVALARAVVREPVVFLLDEP
                     LSNLDAKLRVQTRIELQKQHRALRATFIYVT"
BASE COUNT          478 a          610 c          596 g          388 t
ORIGIN      
        1 ggcgctcaaa cggcctgcat ggcaatatca attcttaagg aggcaacatg aaagacaaac
       61 acaatcaggc ggctggcgaa acgccgcaac tacaaggcgt aacccgccgc cgcttcatca
      121 aagccaccgg cgcggcagga cttgctgcga gcgtcgcccc gttcatcatc gtgccgccgg
      181 cgcgagccgc caaaaagacc ttgaagttac tgcagtggaa ccacttcgtt cccggttatg
      241 acaagtggtt cgacaatacc tacaccaagg agtggggcgc gaaaaacgat actgaggtca
      301 ttgtcgacca tgtgggcatt caggcgctgt cagcgcgcgc cgcagccgaa gtcggggcgc
      361 aaaaaggcca cgacttgtta atgtttctat cccccccgcc cgcttatgaa gaccaggtga
      421 tagaccacaa tgaagtttac caggagtgtg agcgcaagca cggcaagccg gtgccgctcg
      481 ccgtccgcag tacttacaac cccaagacta agaagtattt cgcattctcc gacagctacg
      541 tgcctgatcc ggtgaattgg cgcaaggatt tgtatgacgc cgtgggcgct gctgcgccca
      601 gcacctggga cgacgtgcgc gttgccgggc gcaagatcaa agagaagttc aacattccag
      661 tcggcatcgg tctttcgtca gagcttgaca cagcgatggc catgcgcgcg gcgctctatg
      721 ctttcggcgg ctcgattcag gacgagaaag ccaacgtggt gttgaactcg aagaacacgc
      781 tggaggcggt caagtttgtt aaggccttgt atcaggaaac catgacgccg gaagtgctgg
      841 cctgggatcc gtcgtcgaac aaccgcgcca tgctggccgg ccagatttcg gtggcgttga
      901 atgctatttc gatcacccgc gaaggcgaga acaaaaacat accgggcacc gataatatct
      961 ggctggccaa ggcactggcc ggaccggtgc gccgcatcgg catggagcac gtgatggact
     1021 gctacgtgat ctggaaattc gccgagaaca tcgagggcgc gaagaaattc cttaccgatt
     1081 acattgacaa cttccgtctg ggcttccttg ccagcgagtt ctacaacttc cccagcttcc
     1141 ccagcacggt gtccgacatc gacaagctgc tcgccaatga caccaaggcg catccgagcg
     1201 acaaatacaa agtgctcgcc gatgtgctcg attgggcgac caacgtcggc taccccggtt
     1261 acgccaatgc ggcggaagac gaaatcttca atacctgggt gatcaacacc atgtttgcca
     1321 aggcggcaac cgatactgag acgccggaaa acgccatcaa ggctgcggaa gaacagtgta
     1381 aacgcatctt tgccaagtgg cgtgaaaaag ggctggtctg atccgcccat tccatcgcaa
     1441 cgcaccatcc gccgtacccg gcggatgcgc ttaggtgtcg aattaaaaat aactaacatg
     1501 gccacggtcg aagcacgctc cattaccaaa agttttgacg gcagcaacgc ggtagacggc
     1561 atcaacctgc tggtgaagga aggcgaattc ctggttttgc tcgggccttc cggctcgggc
     1621 aaaagcacct tgctgcgcat gattgccggc ctggaagtgc cgaccgccgg cgatattctg
     1681 atcggcggca agattgtcac cctgtttccg cccaaggcgc gcaacatcgc catggtgttc
     1741 cagagctacg cgctttatcc gcatctcagc gtgttcaaga acatcgcttt tcctttggaa
     1801 gcgcagggca tgaaaagcga ggcggtgaaa aataaagtcg agcacgctgc ggcgatgttc
     1861 ggcatcggcc gcctgctggc gcgcaagccg cgccagctgt ccggcggcga gcggcagcgc
     1921 gttgctctcg cacgcgccgt ggttcgcgag ccggtggtgt tcctgctcga tgagccgctg
     1981 tccaatctcg acgcgaagct ccgcgtgcag acccgcatcg agctgcagaa gcagcatcgg
     2041 gccctgcgcg cgacgttcat ctacgtcacg ca
//
LOCUS       DATIOW010000199         6522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1815129, whole genome shotgun sequence.
ACCESSION   DATIOW010000199 DATIOW010000000
VERSION     DATIOW010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815129"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VLL03_06560"
     CDS             complement(<1..888)
                     /locus_tag="VLL03_06560"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HSS47056.1"
                     /translation="MSDIKKVVLAYSGGLDTSVILKWLQDTYQCEVVTFTADIGQGGE
                     LEPAHAKARQLGVREIFIVDLREEFAQDFVFPMFRANAVYEGEYLLGTSIARPLIAQW
                     QIEIARQTKADAVAHGATGKGNDQVRFELGYYALEPNIRVIAPWREWALTSREKLLAY
                     AKKHGIPVEAQQPGGSPYSMDANLLHISYEGRGLEDPSKEPEESMWRWTVAPEKAPDQ
                     PEYLQLEYEKGDIVSVNGVRLTPAEVLGALNRAGAKHGIGRLDLVENRYLGMKSRGCY
                     ETPGGAIMLKAHRAIESITL"
     gene            complement(992..1909)
                     /gene="argF"
                     /locus_tag="VLL03_06565"
     CDS             complement(992..1909)
                     /gene="argF"
                     /locus_tag="VLL03_06565"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HSS47057.1"
                     /translation="MHVRHFLQFKDFEFHEFEYLFERARTLKARQARGELYQPLIGKT
                     MAMIFEKSSTRTRVSFEAGMNQLGGSAMFLSPRDTQLARGEPIEDVAQVLSRMVDIVM
                     IRTYDQSIIERFAGHSRVPVINGLTNEYHPCQILADIFTYIEQRGPIRGKTVAWVGDS
                     NNVCNSWLQAAKLFDFNFHVATPPGYELEPGRGESYASMHLECFTDPHDAVRGADLVT
                     TDVWTSMGYERETKERKKVFADFRVDEEMMALAKPNALFMHCLPAHRGEEVAAEVIDG
                     PLSAVWDEAENRLHTQKALMEYLLLGKIK"
     gene            complement(1920..3125)
                     /locus_tag="VLL03_06570"
     CDS             complement(1920..3125)
                     /locus_tag="VLL03_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HSS47058.1"
                     /translation="MSHLMNTYTRLPVAFVRGEGVWLWDGQNKRYLDAVAGIGVNGLG
                     HAHPKLVKALAEQVLTLIHTSNLYQVQKQEELAARLADLAHMDKVFFCTSGAEANEAA
                     IKLARLYGHQKGIDNPTIIVMEKSFHGRTMATLSATGSRRVQAGFEPLMGGFARVPYN
                     DVDAVAQVASHNKNIVAVLVEPIQGEAGIRMPLPLPAYLQGLRRVCDEHGWLLMLDEV
                     QSGIGRTGKWFAHQHSGITPNVMTLAKGLGSGVPIGACLAAGQAADVFKPGNHGSTFG
                     GSPLACTAALTTLAVIEEENLMPHAVKLGEFIRNGFAEKLAGAKDVVQIRGEGLMIGI
                     ELAQPCGDLVKLALEQGLLINVTAENVIRLLPSLVMREAEAQQLIDILSGLIQQFVEP
                     GAAAHQARA"
     gene            complement(3222..5204)
                     /locus_tag="VLL03_06575"
     CDS             complement(3222..5204)
                     /locus_tag="VLL03_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSS47059.1"
                     /translation="MRKPVSRILVINDENLVLKELIKGLNAAARALDNPLGITFTGVA
                     TVREALRAIEEDGDLQAVVVDDTLYTLKNDGQNGSRSKQMSALKLVQRITRFRPELDV
                     YILIAREQEDQVVDTLFAESVDGYFYREERDYRGMYRILNAQIQEKARTPFYDQLKSY
                     VLMAKDAWHSPGHSSGDSLRDSPWVSDFYEFTGEHVFDADLSVSVPMLDSLMQPTGVI
                     LEAQKIAAKAFGARRTFFATNGTSTANKVIFQTLIAPGEKLLLDRNCHKSVHHGVVLS
                     GAHPVYLDSSVNRKFGLFGPVPKRTILKAIEEHSNAEALVLTSCTYDGLRYELKPILA
                     AAHAKGIKVVIDEAWYGFARFHPEFRPTALEAGADYAAQSTHKVLSAFSQASMIHVND
                     PDFKEHIFRENFNMHASTSPQYTMIASLDIARKQAVMEGYKLLSRTLGLAKELREQIN
                     STEVFRVLELDDLLPDEVKTDNIRLDPTKVTVDISRCGYTVEDLQRELFDRFNIQVEK
                     STFNTLTLLLTIGTTRSKVSRLYDALMRVAREGRAPRRLYVTPEIPRFTHLKYLPRDA
                     FYCGGELSPLVDEKDQVNTALSGKVCADQIVPYPPGIPVLVPGQMITTETIHYLVGLL
                     RSQKRVELHGVVFDGYLPTLRTLTKAEEKSLKRLRK"
     gene            complement(5490..5753)
                     /gene="rpsT"
                     /locus_tag="VLL03_06580"
     CDS             complement(5490..5753)
                     /gene="rpsT"
                     /locus_tag="VLL03_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HSS47060.1"
                     /translation="MANIASAKKRARQAETRRQHNAGLRAELRTAIKNVRKAIAAGDK
                     AAAQAAFKQAVPVVDCIADKKIVHKNHVARHKSRLSQAIRAMA"
     gene            5830..>6522
                     /gene="murJ"
                     /locus_tag="VLL03_06585"
     CDS             5830..>6522
                     /gene="murJ"
                     /locus_tag="VLL03_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HSS47061.1"
                     /translation="MNLLKALATVSSMTLISRILGFIRDTVIARIFGAGLFADAFFVA
                     FRIPNLLRRLFAEGAFSQAFVPILAEYKNQRGEAATRFLVDHVSGTLALALLVATALG
                     VMAAPVIVYISAPGFSATPDKFAITVELLRITFPYILFISLVSLAGGVLNTWSKFSVP
                     AFTPVLLNLSFIVCALWLAPHVNPPVKALAWAVFCGGVLQLAFQVPFLLRLKMLPRFR
                     PSFNDAGVWRVLK"
BASE COUNT         1204 a         1922 c         1941 g         1455 t
ORIGIN      
        1 gagcgtgata gactcaatcg cccggtgcgc cttgagcatg atggcgccgc ccggtgtttc
       61 atagcaaccg cgcgatttca tgccgaggta gcggttttcc accagatcca gccgcccgat
      121 tccatgcttg gcgccggcgc gattgagcgc tcccagcact tcggcggggg tgagccgaac
      181 gccgttgacg ctgacgatgt cgcctttttc atattccagc tggaggtatt ccggctggtc
      241 cggcgccttt tccggcgcca ccgtccagcg ccacatgctt tcttccggtt ccttggatgg
      301 atcttccagt ccgcggccct cgtaggaaat atgcagcagg ttggcgtcca tgctgtaagg
      361 cgagccgccc ggctgctgcg cctcgaccgg gatgccgtgc tttttcgcat acgccagcag
      421 tttctcgcgc gaggtgaggg cccattcgcg ccaaggtgca atcacccgga tgttgggctc
      481 gagcgcgtaa tagccgagct cgaaacgtac ctggtcgttg cccttgccgg tagcgccatg
      541 cgccaccgca tccgccttgg tctgccgcgc gatttcgatt tgccattggg cgatcagcgg
      601 ccgcgcgatg ctggtgccga gcaggtattc gccttcgtag acggcattgg cgcggaacat
      661 cggaaacaca aagtcctgcg caaattcctc gcgcaggtca acaataaata tttccctgac
      721 cccgagctgc cgcgccttgg cgtgcgccgg ctccagctct ccgccctggc cgatatcggc
      781 ggtgaaagtc actacttcgc actgataggt atcctgcagc catttcaaaa tgaccgaggt
      841 gtccagcccg ccggaatagg cgagcaccac ttttttgatg tcgctcatat atgtgtctct
      901 gattttgatt tatttgtgaa ccgcggagac gccgaggcgc agagttaacc aaaaattcat
      961 attttctccg cgtctccgcg cctctacggt tctatttaat ttttcccagt aaaaggtatt
     1021 ccattaacgc tttttgggtg tgcagccggt tctcggcctc gtcccacacc gcgcttagcg
     1081 ggccgtcgat gacttccgcc gccacttcct cgccgcggtg cgcgggcagg cagtgcatga
     1141 acaacgcgtt gggcttggcg agcgccatca tttcctcgtc cacgcggaaa tcggcgaaga
     1201 ctttcttgcg ctctttggtt tccctttcat agcccatgct ggtccagacg tcggtggtga
     1261 ccaaatcggc gccgcgcacc gcatcgtgcg ggtcggtgaa acattcgagg tgcatactgg
     1321 cgtagctttc cccgcgcccg ggctcgagtt catatccagg cggggtggcc acatggaaat
     1381 tgaagtcgaa cagcttcgcc gcctgcagcc aggagttgca cacgttgttg gaatcgccta
     1441 cccaagccac ggtcttgccg cgtatcgggc cgcgctgctc gatgtaggta aaaatatcag
     1501 cgaggatctg gcaggggtga tattcgttgg tgagcccgtt aatcaccggc acccgcgaat
     1561 gcccggcgaa gcgctcgatg atgctctgat cgtaagtgcg gatcattacg atgtccacca
     1621 tgcgcgacag cacctgcgcc acgtcctcga tcggttcgcc gcgcgcaagc tgggtgtcgc
     1681 gcggggaaag aaacatcgcc gagccgccca gctggttcat gcccgcctcg aaggagacgc
     1741 gcgtgcgcgt cgagcttttc tcgaagatca tcgccatggt cttgccgatg agcggctgat
     1801 aaagctcccc gcgcgcctgc ctggccttga gggtacgcgc gcgctcgaac agatactcga
     1861 actcgtggaa ctcgaaatcc ttgaattgga gaaaatgcct gacgtgcatg ggcggtgtct
     1921 caggcgcgcg cctggtgggc ggccgcgccg ggttccacaa attgctgaat gaggccgctt
     1981 aatatgtcga tgagttgctg cgcctcggct tcgcgcatga ccagcgacgg caacaggcgg
     2041 atcacgtttt ccgcagtcac gttaatgagc aagccttgtt cgagcgccag cttgacgaga
     2101 tcgccgcaag gctgcgcgag ctcaatccct atcattaagc cttcgccgcg gatttgcacc
     2161 acgtcttttg cgcccgcgag cttctctgca aaaccgttgc ggatgaattc gccaagcttt
     2221 accgcatgcg gcatcaggtt ttcttcttca atcaccgcca atgtggtgag cgccgcggta
     2281 caggcgaggg ggctcccgcc gaaagtcgaa ccgtgattgc ccggcttgaa cacatcggcg
     2341 gcttggcctg ccgcaagaca cgcgccaatc ggcacgcccg agcccagacc cttggcgagc
     2401 gtcatcacat tcggcgtgat tcccgaatgt tggtgagcga accatttacc ggtgcggccg
     2461 atgccgcttt gcacttcgtc aagcatcaac agccagccgt gctcgtcgca aacgcggcgc
     2521 aggccttgga ggtaagcggg caggggcagc ggcatgcgta tcccggcttc gccctgaatc
     2581 ggttccacca gcacggcgac gatgtttttg ttgtggctcg ccacttgtgc caccgcatcc
     2641 acatcgttgt agggaacgcg cgcaaagccg cccatcagcg gctcaaaccc cgcctgcacc
     2701 cgccgcgagc cggtggcgga gagcgtcgcc atggtgcgcc cgtggaaact tttttccatg
     2761 acgatgatgg ttgggttatc gatgcctttc tggtggccgt atagccgcgc cagcttgatc
     2821 gccgcttcat tggcttccgc ccccgaagtg cagaaaaaca ccttgtccat atgagcgagg
     2881 tcggccagcc ttgccgctaa ttcttcttgc ttctgcacct ggtacagatt ggaggtgtga
     2941 atcagcgtaa gcacctgctc ggcgagcgct tttaccaatt tggggtgcgc atggccaaga
     3001 ccgttcaccc cgatgccagc gacggcgtct aggtaacgtt tgttttgccc gtcccacaac
     3061 catacgcctt cgccacggac gaaggcgacc ggcaaccggg tataggtgtt catcagatgc
     3121 gacataacgt ttaccgaaaa acaaatgcac acgggagcat gcgccgccgt gtgccggggt
     3181 ttatcaagtc ttcactaaat tatacgcttg ttgcaagaaa attatttacg cagccgcttc
     3241 aagctctttt cctcggcttt ggtgagggtg cgcagggtgg gcagataacc gtcgaaaaca
     3301 acgccgtgca gctcgacccg tttctggctg cgcagcaaac ccaccaggta gtggatggtt
     3361 tcggtggtaa tcatctgtcc cggcaccagc accggaatgc cgggcgggta ggggacgatc
     3421 tggtcggcgc agaccttgcc ggagagcgcg gtattgacct ggtctttctc gtccaccagc
     3481 ggcgagagtt ccccgccgca atagaaagcg tcgcgcggca ggtacttgag gtgggtgaag
     3541 cgcgggattt caggcgtcac gtaaagccgg cgcggcgcgc ggccctcacg cgcgacgcgc
     3601 atcagagcgt catacaggcg cgacaccttg ctgcgcgtgg tgccaatggt aaggagaagc
     3661 gtcagcgtat tgaacgtgga tttttccacc tgaatgttga agcggtcgaa cagttcacgc
     3721 tgcaaatctt ccacggtgta gccgcagcgc gaaatgtcca cggtgacctt ggtggggtcg
     3781 agccggatat tgtctgtttt cacttcgtcg ggcagcagat cgtcgagctc gagtacgcgg
     3841 aacacctcgg tggaattgat ttgctcgcgc agttccttcg ccagccccag cgttcttgag
     3901 agcagcttgt agccttccat caccgcctgc ttgcgcgcga tgtcgaggct cgcaatcatc
     3961 gtgtattgcg ggctggtgga ggcgtgcatg ttgaagtttt cgcgaaagat gtgctccttg
     4021 aaatccgggt cgttgacgtg aatcatcgag gcttgcgaaa atgccgagag taccttgtgc
     4081 gtgctttgag cggcgtaatc ggcgccggct tccagcgcgg tgggacggaa ctcgggatga
     4141 aagcgcgcaa agccgtacca ggcttcgtct atcaccacct tgatgccttt tgcgtgcgcg
     4201 gcagcgagga tgggtttcaa ttcgtagcgc aagccgtcgt aagtgcagct ggtcagcacc
     4261 agcgcttcgg cattggaatg ctcctcgatg gctttcagga tggtgcgctt cggcaccggg
     4321 ccgaacagtc cgaatttgcg gttgactgag gaatcgagat aaaccggatg cgcgccggag
     4381 agcaccacgc cgtggtgcac ggatttatgg cagttgcggt cgagcaaaag cttttcgccg
     4441 ggagcgatca gcgtctggaa aatcactttg ttggcggtgg aggtgccgtt ggtggcgaaa
     4501 aacgtgcgcc gcgcgccaaa agccttggcc gcgattttct gcgcctccag aatcacgccg
     4561 gtgggctgca tcagcgaatc gagcatcggc accgacactg acaaatccgc atcgaacaca
     4621 tgctcgccag tgaattcgta gaaatcgctg acccacgggc tgtcgcgcaa actgtcgccg
     4681 ctggagtgtc ccggcgaatg ccaggcatcc ttggccatca gcacatagct tttcaactgg
     4741 tcgtaaaacg gcgtgcgtgc tttctcctga atttgcgcat tgagaatccg gtacatgccg
     4801 cggtaatcgc gctcttcgcg gtaaaaataa ccatcaactg attccgcgaa cagagtgtcc
     4861 accacctggt cttcctgctc cctggcaatt aggatgtaga cgtcaagctc gggacggaag
     4921 cgggtgattc gctgcaccag cttcagcgcc gacatctgct tggagcgcga gccgttctgt
     4981 ccgtcgtttt tcagggtgta gagtgtgtcg tccaccacca ctgcctgcag gtcgccgtct
     5041 tcttcaatcg cgcgcaacgc ctcgcgcacg gtggcgacgc cggtgaaagt gatgccgaga
     5101 gggttatcga gcgcgcgcgc cgcggcatta aggcccttga tgagctcctt caggaccaga
     5161 ttttcgtcgt tgatgacgag gatacggctt accggcttgc gcatgactgt tgcggattaa
     5221 aaaagacgct ttatgcgcca aatgcgcgcg caatgcaagc cccggctata atgagccgtg
     5281 ccaaaaacta cttccaacag tctgccggcg gaaatcgtca tccatgggat caccgcaaac
     5341 ggccagatct tccggcccag cgactggccg gctgaattat cggtgataca tttggctctg
     5401 ttcgctcttc gacgcgctca gagcgaacgg acgcaaggac tcaccacgaa cgcgaaaatc
     5461 cggaataagc gggttatgcg aagaggtact taagccattg cccggatggc ttgtgacagg
     5521 cggcttttgt ggcgggcgac gtggttcttg tgaacgattt ttttgtcggc gatgcaatcc
     5581 accactggca cggcctgttt gaaagcggct tgcgccgcgg ccttgtcgcc ggcagcaatg
     5641 gctttgcgca cattcttgat ggcggtgcgc agctcggcgc gcagtcccgc gttgtgctgg
     5701 cgccgcgtct cggcttgccg ggcgcgtttc ttggcggatg cgatattggc catgtaaaca
     5761 cctgaaaccg tgaaagggcg aaatgatacg tttgttcggg cttctttgca accacaatcc
     5821 ggtcccgtta tgaatttatt aaaggcgctc gccacggtca gcagcatgac cctgatctcc
     5881 cgcatcctgg gtttcatcag ggacaccgtg atcgcccgga ttttcggagc gggcttgttc
     5941 gccgatgcat tcttcgtcgc cttccgcatc cctaatctgc tgcgccggct gtttgctgaa
     6001 ggcgcgttct cgcaggcttt cgtgccgata ctcgccgaat acaagaacca gcgcggcgag
     6061 gccgcgaccc ggtttctggt ggaccatgtc tcgggaacac tcgcgctcgc cttgttggtg
     6121 gcgacggcgc tcggcgtcat ggcggcgccc gtcattgttt acatcagcgc gccgggattt
     6181 tccgccacac ccgataaatt cgccatcacg gtggaactgc tgcgcatcac tttcccctat
     6241 attctgttta tctcgctggt gtcgctggcc ggaggcgtcc tcaacacctg gagtaagttt
     6301 tcggtgccgg cgttcacccc ggtgctgctt aacctttcgt tcatcgtctg cgctttgtgg
     6361 cttgcgcccc atgtgaaccc gccggtgaaa gcgctggcct gggcggtgtt ttgcggcggc
     6421 gtattgcaac tcgcgtttca ggtgccgttt ctgctgcgtc tgaaaatgct gccgcgtttc
     6481 cggccgagtt tcaatgacgc cggcgtgtgg cgcgtgttga ag
//
LOCUS       DATIOW010000200        14980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1509491, whole genome shotgun sequence.
ACCESSION   DATIOW010000200 DATIOW010000000
VERSION     DATIOW010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14980
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509491"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..403
                     /locus_tag="VLL03_06590"
     CDS             212..403
                     /locus_tag="VLL03_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS47062.1"
                     /translation="MKLFTNLAIALLILIAIVHLLRLVFGWEVTLNGMLVPMWVSVLG
                     LLIPGALAFFLWRESRKNK"
     gene            complement(451..639)
                     /locus_tag="VLL03_06595"
     CDS             complement(451..639)
                     /locus_tag="VLL03_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS47063.1"
                     /translation="MPVKNDPDKIKGPGGLTLRQIHEQVKRSAERDRQAEKTRNALLQ
                     KQSRWQRFVRYVWDRKSK"
     gene            706..1866
                     /gene="aroC"
                     /locus_tag="VLL03_06600"
     CDS             706..1866
                     /gene="aroC"
                     /locus_tag="VLL03_06600"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HSS47064.1"
                     /translation="MSGNTLGRLFCVSSFGESHGPAIGCVVDGCPPGMALSVEDIQKE
                     LDRRKPGTSRHVTQRRESDAVEILSGVFEGKTTGTPIALLIRNEDARSRDYSAIMDTF
                     RPGHADYTYWQKYGIRDYRGGGRQSARETAVRVAAAAIAKKWLREKYGVMIRGYMSQL
                     GPIEIPFKTWDAVNSNPFFAADASFVPKLEEFMDKLRKSGDSCGAKITTIAEHVPVGW
                     GEPVYDKLDADIAYAMMSINAVKGVEIGAGFASVEQKGSEHSDEMTLEGFLSNNAGGI
                     LGGISTGQDIVVNIAVKPTSSIRLPRRSIDKQGKPAIVETHGRHDPCVGIRATPIAEA
                     MLVLVLMDHALRHRAQNADVKCPTPKIPAKAGKSAALKGKVVHKENPEPDEA"
     gene            1886..3046
                     /locus_tag="VLL03_06605"
     CDS             1886..3046
                     /locus_tag="VLL03_06605"
                     /inference="COORDINATES: protein motif:HMM:NF037955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSS47065.1"
                     /translation="MSPRSTARNFPYWRLSSFYFFYFAFVGAFSPYWSLYLKSLDFDA
                     AEIGVLMSLLLVMRIFAPTLWGWLADHTGKRVGIVQIGAALSVFCYLGVFFGTSFWWM
                     FVVMALISFFWSASLPLVEATTLSHLGDSSASYGQVRLWGSIGFIVAVIIIGYVLDFA
                     PLRILLWLVLGTMVGILFFSRYIPEAEVHTHHTDHLPVSQIFKRPEVLALFAACFLMV
                     AAHGPFYTFYSLYLVAHGYSKSVIGWLWAVGVICEIVVFIFMPRLTRHYSLAQILSVS
                     FASAVARFLLIGWLVDSTVAIFFAQVLHAATFGSYHAAAVAAIHHFFRGRHQATGQAL
                     YTSLTFGAGGTFGALMSGYAWESMGPSLTFSMASFFALLGLILISWKFRLKA"
     gene            3111..4520
                     /gene="leuC"
                     /locus_tag="VLL03_06610"
     CDS             3111..4520
                     /gene="leuC"
                     /locus_tag="VLL03_06610"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HSS47066.1"
                     /translation="MGAQTLYDKLWASHLVQEQADGTAFIYIDRHLVHEVTSPQAFEG
                     LRLAGRKPWRMDSVVATADHNTPTMDWDEGIKDPVSRLQVATLDANIRQYGAKAYFPF
                     RDRSQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFAALAFGIGTSEVEHVLATQCL
                     QMKKSRAMQIVVEGELGRGVTAKDIVLAIIGKIGTAGGTGYVIEYAGSVIRALSMEAR
                     MTICNMSIEAGARAGMVAVDDTTLNYLKERHFAPKDAMWDKAVAYWKTLKSDEGARFD
                     KVVTLNATGIKPQVTWGTSPEMVGTIDDRVPDPARVADPVKREAMERALKYMGLQPNT
                     PIQKIKLDKIFIGSCTNSRIEDLRAAAQIVRGKHVAASIKQALVVPGSGLVKRQAEAE
                     GLDQIFREAGFEWREPGCSMCLGMNDDRLQPGERCASTSNRNFEGRQGTGGRTHLVSP
                     QMAAAAAIAGHFVDVRGKF"
     gene            4541..4675
                     /locus_tag="VLL03_06615"
     CDS             4541..4675
                     /locus_tag="VLL03_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HSS47067.1"
                     /translation="MLKIVLLATVLVAVLAGCNTMEGLGKDLSKLGDKIEKKADEKKN
                     "
     gene            4678..5316
                     /gene="leuD"
                     /locus_tag="VLL03_06620"
     CDS             4678..5316
                     /gene="leuD"
                     /locus_tag="VLL03_06620"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HSS47068.1"
                     /translation="MEKFTRLDGLVAPLDRANVDTDSIIPKQFLKSIKKNGFGPNLFD
                     AWRYLDTGEPGKDNLGRPLNPDFVLNAPRYQGAQILLARENFGCGSSREHAPWALLDY
                     GFRALIAPSYAEIFYSNSLKNGLLPIVLDSKAVDQLFREVAAQPGYRLKVDLQAQSVT
                     TPSGQTFSFSIDPHQKHCLLNGLDEIGLTLQHADKIRAFEAQHRARQPWLFS"
     gene            5483..6547
                     /gene="leuB"
                     /locus_tag="VLL03_06625"
     CDS             5483..6547
                     /gene="leuB"
                     /locus_tag="VLL03_06625"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HSS47069.1"
                     /translation="MKIAVLPGDGIGTEIIAQALKVLQRLARDGLKIEMECAPVGGAG
                     YDTHGDPLPEATLRLAQDADAILFGAVGGYQYDTLPRPQRPEQGLLRIRKALGVFANL
                     RPALLYPELTQASTLKPEVVAGLDIMILRELTGDVYFGEPRGRRKNAKGDREGFDTMV
                     YSESEIRRIAQVGFNIARKRKRKLCSVDKANVLETSQFWREIVTEVGKQYPDVELSHM
                     LVDTAAMQLVRNPRQFDVILTGNLFGDILSDQASMLTGSIGMLPSASLDANNKGLYEP
                     IHGSAPDIAGKDIANPLAAILSAAMMLRYTFNLEEAASRIEQAVKKVLRQGYRTADIL
                     EPGLKQVGTEEMGDAVVGAM"
     gene            6647..7672
                     /locus_tag="VLL03_06630"
     CDS             6647..7672
                     /locus_tag="VLL03_06630"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HSS47070.1"
                     /translation="MSKKYDVCVLGATGVVGEAMFAILEERDFPVRNLYPLASSRSAG
                     TSIRFKGKDIEVIDVSGFDFSKVRIGLFSAGASVSAEYAPKAAAAGCVVIDNTAQFRY
                     DKDIPLVVPEVNPHAIAQYKNRGIIANPNCSTIQLVVALKPIHDAVGIVRINVATYQS
                     VSGTGKEAIEELAQQTRDLFNHKDPVAEVYPKRIAFNVLPHIDAFQDNGYTKEEMKMV
                     WETNKIMEDNSIKVNPTTVRVPVFYGHSEAVHLETRNKITAREATELLKKAPGVVVLD
                     ERKPGGYPTPVTEAAGRDPVYVGRIREDISHPQGIDLWVVSDNVRKGAALNSIQIAEI
                     LIEKYLD"
     gene            7813..10401
                     /locus_tag="VLL03_06635"
     CDS             7813..10401
                     /locus_tag="VLL03_06635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HSS47071.1"
                     /translation="MPCIAHAVGLGRLTVHSSLGQALDAEVELLGVQKGELDTLAAKL
                     ASAEAFRKANVEFNPALPAIKFSIEKRPDGRPYLKLKTLHPVNEPFLDFLIELNWASG
                     RLVKEYTALLDPPGYEPRPTLAPAAPPAPVALPEAQPLPEEAPKPEVAGVPPDEKVGA
                     EAQAAPEEGETAAPQEAAVVPETPAPQAEAVEEKKTYGPIKRGETLRKIAESVKPVDY
                     SLEQMLVGLFRSNHEAFMGNNMNRMKTGPILRVPENTELSTIDPNDAVKEVRVQARNW
                     NAYRQKLAEAVGQATPAATAKQSASGEISTKIEEKAAPKEPGKEVLKLSKGEAGTGGK
                     PAAGDKALQERLRSLEEEIIAREKAVKEANERITMLEKNIRDMQKLLELKNQNLAEVQ
                     KQAGAAKPQAAQPAPQPQPAPKPAETKPAEKPAQPQLAARPAAKPEQKPAQVAQQSPP
                     QDKPKPKIITPPPEVSFLDDILDNPLYLGGGAGLIIALLGLGYIAVRKKKSITRFEDS
                     AISSDLKTSTVFGNTEGGTVNTGDSSIMSDFAGKIGGTINTDDVDPLAEAEVYIAYGR
                     DTQAEEILKEALKKDPTRQQIHLKLLEILANHKNVSAFESIASDLYAACGGKGLLWDK
                     AVYLGYKIDPNNPMYASAKGAAVEMREETAAPPGPPHMDFNVGVPSTGEPVEVQPDIA
                     IDTVAPAAPDNVDISFEIPTSETAKTIPDIPFGIAQPADDETDFNLGSIGAETPLAAE
                     PLKPSQEAEPMPEMISYNFGPSKPAEAPAPAPATSEADAVHIDFSSISLNLDEPAAAP
                     AAVPGAAEEAAKDQHWYDVQTKYDLAKAYFEMGDKEGAREILQEVAQEGDAKQQEAAK
                     GLLAQL"
     gene            10480..11037
                     /locus_tag="VLL03_06640"
     CDS             10480..11037
                     /locus_tag="VLL03_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HSS47072.1"
                     /translation="MLAIGVQGLHSVGLAALSLLLLVVACRFHGFPRLLKRTRWLFLS
                     LLVIYAIATPGELLWPQVGAASPTVEGLREGALQAWRLLCLLAALAWLLAGTPRAALI
                     GGLYRMLHPFFGNHAGRIAVRMALTLDYAEQAAGVENWKNAFNAEFDGTDTVSTISLR
                     LEPFTWRDVVVLAGVLLALGALRAW"
     gene            11059..11841
                     /gene="truA"
                     /locus_tag="VLL03_06645"
     CDS             11059..11841
                     /gene="truA"
                     /locus_tag="VLL03_06645"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HSS47073.1"
                     /translation="MRIALGLEYNGAAFCGWQSQPGGCAVQDAVEAALSQIAGEKLSL
                     TAAGRTDAGVHALSQVAHFDTAVDRPGTAWVRGVNALLPQSVAALWAQPVADDFHARF
                     SAIERCYRYVLLNTAVRPALHEGKAGWFHQPLDMEKMREAATVLLGKHDFSAFRAAEC
                     RARSPIKELRRIDIMRQQDWLVFEFCADAFLHHMVRNIVGCLVYVGSGRCPVRWLDEV
                     LQSRDRSTAAPTFSPVGLYLSAVRYEAKWRLPDFNRTMLLFP"
     gene            11850..12473
                     /locus_tag="VLL03_06650"
     CDS             11850..12473
                     /locus_tag="VLL03_06650"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HSS47074.1"
                     /translation="MPTRVKICGITRVQDGAAAGQLGAHAVGLMFYEKSPRFVSLETA
                     REVIAALPPFVARVGVFVNPDEAQVRAVLDALRLDFLQFHGDEAPEFCRRFGAPYLKA
                     VRVKKGVDLLQYASRYHEAKGLLLDAHSESVYGGTGEAFDWRLIPRRLPLPVILSGGL
                     NVANVAAAILQVKPWAVDVSSGVESAKGIKDAAKIAAFIKEARDADV"
     gene            12466..13659
                     /gene="trpB"
                     /locus_tag="VLL03_06655"
     CDS             12466..13659
                     /gene="trpB"
                     /locus_tag="VLL03_06655"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HSS47075.1"
                     /translation="MYDLPDEHGHFGKYGGVFVAETLIHALDQLREGYQRYRNDAEFQ
                     AEFADELKHYVGRPSPVYHAKRWSRELGGAQILLKREDLNHTGAHKINNTVGQALLAR
                     RMGKPRVIAETGAGMHGVATATVAARYGMECVVYMGSTDIKRQATNVYRMKLLGASVV
                     PVESGSKTLKDALNEAMRDWVTNVENTFYIIGTVAGPHPYPMMVRDFQSIIGREAKTQ
                     MQEMFGRQPDAAIACVGGGSNAMGLFYPYIEEKSVRLIGVEAGGLGLPSGKHAATLCA
                     GKPGVLHGNRTYLMQDENGQIIETHSISAGLDYPGVGPEHAWLKDSGRAEYVNVTDDE
                     ALKAFHDLCHMEGIIPALESAHALAYAAKIAPQMGREQLLLVNLSGRGDKDMNTVANL
                     SGIKF"
     gene            13758..14561
                     /gene="trpA"
                     /locus_tag="VLL03_06660"
     CDS             13758..14561
                     /gene="trpA"
                     /locus_tag="VLL03_06660"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HSS47076.1"
                     /translation="MSRIAATFSRLRQQKKKALIPFITAGDPEPGMTVKMMQTLSEGG
                     ADIIELGVPFSDPMADGPTIQRSSERALKHRISLKQILDMVAEFRKFNPHTPVVLMGY
                     ANPIETLGYEKFAQAAQVSGVDGVLVVDYPPQECEELVKHLERCGIDPIFLLSPTTTE
                     ARIEEVAKLSRGYLYYVSLKGVTGARHLDLSDVAKKLPQIRARTKLPVGVGFGIRDAQ
                     TAKAVAALADAVVVGSRIVEEVEKSAKDQVLGKIAVLIQDLRRAIDQPG"
     gene            14566..>14980
                     /locus_tag="VLL03_06665"
     CDS             14566..>14980
                     /locus_tag="VLL03_06665"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399590.1"
                     /note="catalyzes the carboxylation of acetyl-CoA to
                     malonyl-CoA; forms a tetramer of AccA2D2 subunits; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit beta"
                     /protein_id="HSS47077.1"
                     /translation="MSWLQKLLPPKIKRTPSGTKKTVPEGLWTKCSKCEAVLYRSELE
                     NNFNVCPKCGNHHRLNARERLEALLDAEGRYEIGAEVMPVDTLKFKDDKRYNERLEQA
                     QEETGESEALVVMQGSIKTVPLVAAAHEFRFMGGSM"
BASE COUNT         3249 a         4215 c         4540 g         2976 t
ORIGIN      
        1 gccctgctct gcgctttgcg agcgctggcc cgcccccgct gcacagcttc gcaggtgcaa
       61 cgctaggcgg gccctccgtc gtagtctcag ctacgacctt cgtcgcgcgc cttgcatcca
      121 tccgcatttt gccggcaacg caatccgtag agaacttaat tagaagctcc ctagccttaa
      181 agcgtgactg aatttatgca ggagaaagca catgaaactg ttcactaacc tcgccattgc
      241 gcttttgatc ctgattgcga tcgtgcatct attgcggctt gtttttggct gggaggtcac
      301 cctgaacggt atgctggtac ccatgtgggt gagcgtattg gggctattaa ttcctggtgc
      361 gctggctttt ttcctctggc gcgagagccg caagaacaaa tagccgctgc accaggacga
      421 ttttgccgct ggcggtcatc agcaccgccc ttattttgat ttccggtccc aaacatagcg
      481 cacgaagcgc tgccagcggc tttgcttttg caaaagcgca ttgcgggttt tttcggcttg
      541 gcggtcgcgc tcggcgctgc gcttcacttg ttcgtgaatc tgccgcaggg tcaatccgcc
      601 cgggcccttg attttgtccg gatcgttctt tacaggcatg gcttattcgc gaatggaata
      661 ggagatatat gttaaacttt agcgacattt ttttcgagaa aaatcatgtc gggtaatact
      721 ttagggcggc ttttttgcgt gtcttccttc ggcgagtcgc acgggccggc gatcggctgc
      781 gtggtggatg gctgcccgcc cggaatggcg ctctccgtcg aggatatcca aaaggaactc
      841 gatcggcgca agcccggcac ctcgcgccat gtcacgcagc ggcgcgaatc cgacgcggtg
      901 gaaatcctct ccggcgtatt tgaaggcaaa accaccggca cgccgattgc gctcctgatt
      961 cgcaacgagg atgcgcgctc cagggattac agcgccatca tggatacttt ccgtccggga
     1021 catgccgatt acacctactg gcagaaatac ggcattcgcg attaccgcgg gggcggccgg
     1081 cagtcggcgc gcgaaaccgc ggtgcgcgtt gcggcggcgg cgattgcaaa aaaatggctg
     1141 cgcgaaaaat acggcgtgat gatccgcggc tacatgtcgc agctgggtcc gatcgaaatc
     1201 ccgttcaaaa cctgggacgc ggtgaacagc aatccgtttt tcgccgcgga cgcgagcttc
     1261 gtgcccaagc ttgaggaatt catggacaag ctcaggaaat cgggcgattc ctgcggtgcg
     1321 aaaattacca cgattgccga gcatgtgccg gtgggctggg gcgagccggt gtacgacaag
     1381 ctcgacgccg acatcgccta tgccatgatg agcatcaacg cggtgaaagg cgtggaaatc
     1441 ggcgcgggct tcgcgagcgt ggaacagaaa ggcagcgagc attccgatga aatgactctc
     1501 gaaggttttc tgtcgaacaa cgccggcggc attctcggcg gcatttccac cggccaggac
     1561 atcgtggtca atatcgcggt gaagcccacc tccagcattc ggctcccgcg ccgctccata
     1621 gacaaacagg gcaagcccgc tattgtcgaa acgcacggcc gccacgatcc ttgcgtcggc
     1681 atccgcgcca ctcccatcgc cgaagccatg ctggtgctgg tgctgatgga tcatgcgttg
     1741 cgtcaccgtg cgcaaaacgc tgatgtgaaa tgtcccacgc ccaaaattcc ggcaaaggcc
     1801 ggcaaaagcg cagctttaaa gggcaaagtt gtgcacaagg aaaatcccga gccggatgag
     1861 gcttaaattt ttgagtcgcc ctgcggtgtc tccacggagc accgcgagaa actttcccta
     1921 ctggcgcctt tccagcttct acttttttta ttttgccttc gtcggcgcat tttcccccta
     1981 ctggagcctg tacctcaaat cgctggattt cgacgccgct gaaatcggcg tgctgatgtc
     2041 gctgcttcta gtgatgcgca ttttcgcgcc cacgctgtgg ggctggctgg cggatcacac
     2101 gggaaaacgc gtcggcattg tgcagattgg cgccgcgctt agcgtcttct gttatctcgg
     2161 cgtgtttttc ggcaccagct tctggtggat gttcgtggtg atggcgctca tcagcttttt
     2221 ctggagtgcc tcactgccgc tggtggaagc gaccaccttg agccatcttg gcgacagcag
     2281 cgcgagctac gggcaggtga ggctgtgggg ttccatcggt ttcatcgttg cggtcattat
     2341 cattggctat gtgctggact ttgcgccgct gcgcattctc ctttggctgg tgttgggcac
     2401 aatggtggga attctatttt tctcgcgcta catccccgaa gcggaagtcc acacgcacca
     2461 taccgaccat ctgccggtat cgcagatatt caagcgccct gaagtgctgg cgctgttcgc
     2521 cgcctgtttc ctgatggtgg ctgcacacgg gccgttttat acattttatt cgctctacct
     2581 tgtggcgcac ggctacagca aaagcgtcat aggctggctg tgggcggtgg gggtgatttg
     2641 cgaaatcgta gtgttcatct tcatgccgcg cttgacgcgg cattattcgc tggcgcagat
     2701 tttaagcgtg agctttgctt cggcggtcgc gcgttttctg ttgatcggct ggctggtgga
     2761 ttcgactgtc gcgatttttt tcgcgcaagt gctgcatgcg gcgacttttg gctcctacca
     2821 tgccgccgcg gtggcggcga tccatcattt tttccgcggc cggcatcagg ccacagggca
     2881 ggcgctctat accagcctca ctttcggggc cggtggaacc ttcggcgcgc tgatgagcgg
     2941 atacgcctgg gaaagtatgg ggccgagcct caccttcagc atggcctcgt ttttcgcgct
     3001 gttggggctg atactgatta gctggaaatt caggctgaaa gcgtgaattg aaacctgttc
     3061 cttgctgtct caatatgaga taatttcaaa tttggtttgg ggcagttttt atgggcgcgc
     3121 aaactcttta cgacaagctt tgggcaagcc atctggtgca ggaacaagcc gatggcacgg
     3181 ccttcattta catcgaccgc catctggtgc acgaagtcac cagcccgcag gcgttcgagg
     3241 ggctgaggct cgccggaaga aaaccctggc gcatggactc ggtggtggca accgccgacc
     3301 acaatacgcc gaccatggat tgggacgaag ggatcaaaga cccggtctcg cgcctgcagg
     3361 tcgcgacgct ggacgccaac atccgccagt atggcgccaa agcctacttt cccttccgtg
     3421 accgcagcca gggcatcgtg catgtgatcg gcccggagca gggcgcgacg ctgcccggca
     3481 tgacggtggt gtgcggcgat tcgcacacca gcacccacgg cgccttcgcc gcgctcgctt
     3541 tcggcatcgg cacctcggaa gtcgagcatg tgctggcgac gcagtgcctg cagatgaaaa
     3601 aatccagggc gatgcagatt gtcgtggaag gcgagctggg ccgcggcgtg acggcgaaag
     3661 atatcgtgct agccatcatc ggcaaaatcg gcaccgccgg cggcaccggc tatgtgattg
     3721 aatatgccgg cagcgtcatc cgggctttaa gcatggaagc gcggatgacg atttgcaaca
     3781 tgtccatcga agccggcgcg cgcgccggca tggtggcggt agacgatacc accctcaatt
     3841 acctcaagga gcgccatttc gccccgaaag atgcaatgtg ggacaaggcc gtggcctatt
     3901 ggaagacgct caagagcgac gagggtgcgc gctttgataa agtggtgact ctgaacgcca
     3961 ccggcatcaa accgcaggtg acctggggca cctcgccgga aatggtgggc acgatcgatg
     4021 atcgtgtgcc tgatccggcg cgtgtagcgg atccggtgaa gcgcgaagca atggagaggg
     4081 cgctgaaata catggggctg caaccgaaca cgccgattca gaagatcaag ctcgacaaga
     4141 tttttatcgg ctcgtgcacc aactcgcgca tagaggattt gcgcgcggcg gcgcaaatcg
     4201 tgcgcgggaa gcatgttgcg gccagcatca agcaggcact ggtggtgccg ggctccggct
     4261 tggtcaagcg ccaagcggaa gcggaagggc ttgaccaaat tttccgcgaa gccggctttg
     4321 agtggcgtga gccgggctgc tcaatgtgtt tgggcatgaa tgacgaccgc ctgcagccgg
     4381 gagagcgctg cgcctccact tcgaaccgca atttcgaagg gcgacagggc acgggcggca
     4441 ggacgcatct agtgagcccg caaatggcgg cggcggcggc gattgccggg cattttgtcg
     4501 atgtgcgcgg aaaattttga tggtaaagaa aggacgcagc atgctgaaaa tcgttttgct
     4561 tgcaaccgtg ctggttgcgg tgctcgcggg ctgcaatacc atggagggtt tgggcaagga
     4621 cctctccaag cttggtgaca agattgaaaa gaaagccgac gagaagaaaa actaatgatg
     4681 gaaaaattca cccggcttga cgggctggtg gcgccgctgg atcgcgccaa tgtcgatacc
     4741 gattccatca tccccaagca atttctgaaa tccatcaaga agaacggctt cgggccgaat
     4801 ctgttcgatg cttggcgcta cctcgacacg ggcgagccgg gcaaagacaa cctcggccgc
     4861 ccgttgaatc cggatttcgt gctgaatgcg ccgcgctacc aaggcgcgca gattttgctt
     4921 gcgcgcgaaa acttcggctg cggctcgtcg cgcgaacacg cgccgtgggc gctgctcgat
     4981 tacggcttcc gcgcgctgat tgcgccgagc tacgccgaaa ttttttacag caacagtctg
     5041 aaaaacggct tgctgccaat tgtgctggac tccaaagcgg tggaccagtt gttccgcgaa
     5101 gtggcggcgc aacccggcta ccggctcaag gtggatttgc aagcgcagag cgtgaccacg
     5161 ccctcagggc aaactttcag cttcagcatt gacccccacc aaaaacactg cctgctcaac
     5221 gggctcgacg aaatcgggct gactttgcag cacgccgaca agatcagggc gttcgaggcg
     5281 cagcaccgcg cccgccagcc ctggttgttt tcctgagttt tcaccacaga ggcacaggga
     5341 gaaagaggaa aaacttaatt tcacaggatt aacaggattc acaagattca ccctgttaat
     5401 cttgcgaaat cttgtaaatc ctgtctgtta ttttttgttt cattcgtgcc tctgtgtctc
     5461 tgtgattagt taagggttaa acatgaaaat tgcggttttg cctggcgacg gcatcggtac
     5521 tgaaatcatc gcgcaagcgc taaaggtatt gcagcggctg gcgcgcgatg ggctgaaaat
     5581 tgaaatggaa tgcgcgccgg ttggcggcgc gggttatgac acgcacggcg atccgctgcc
     5641 ggaggcgacg ctcaggctcg cgcaagatgc ggatgcgatt ttgttcggcg cggtaggcgg
     5701 ctatcaatac gacacgctgc cacgcccgca gcggccggag caagggcttt tgcgtatccg
     5761 caaggcgctc ggcgtattcg ccaatttgcg cccggcgcta ctgtatcccg agctcaccca
     5821 ggcttccact ttgaaaccag aagtggtggc ggggctcgac atcatgattt tgcgcgagct
     5881 gaccggcgat gtgtactttg gcgagccgcg cggacgaaga aagaatgcca aaggcgatcg
     5941 cgaaggtttt gataccatgg tgtactccga atcggagatc cgccgtatcg cgcaggtggg
     6001 ttttaacatc gcgcgaaagc gcaaacgcaa gctctgctcg gtggacaaag ccaatgtgct
     6061 tgagaccagc cagttctggc gcgagatagt gacggaagtg ggcaagcaat atcccgacgt
     6121 cgagctttcg catatgttgg tggacaccgc ggcgatgcag ctggtgcgaa accccaggca
     6181 gttcgacgtg attctgaccg gcaacctgtt cggggacatt ctgtccgacc aggcatcgat
     6241 gctgaccggc tccatcggca tgctgccttc ggcctctctc gatgccaaca acaaggggct
     6301 ctacgagccg attcatggct cagcgccgga catcgctggc aaagacattg ccaaccccct
     6361 ggccgccatc ctctcggcgg cgatgatgct gcgctatact ttcaacctcg aagaggcggc
     6421 aagccgtatc gagcaggcgg tgaaaaaagt attgaggcag ggctaccgca ccgcggatat
     6481 acttgagccc ggcctgaagc aggtcggcac agaggaaatg ggcgacgcgg tggtgggtgc
     6541 aatgtgacga ttacctaaac cgccgaggcg cggagacgaa aaccgaattg ctccgtgttt
     6601 ctgcgtcttt gcgggaattt aactgttgaa ccgaggaaaa aaatagatga gcaagaagta
     6661 cgatgtatgt gtgctgggtg cgaccggcgt ggtcggcgaa gcgatgtttg ccattctgga
     6721 agagcgcgat tttccggtgc gcaatcttta tccgctggcc tcttcgcgtt ccgcagggac
     6781 ctcgatccgc ttcaagggca aggacatcga agtaattgat gtgtccggct ttgatttttc
     6841 caaggtgcgg atcggcttgt tttcggcggg cgcttccgtg tcggccgaat acgcgccgaa
     6901 agccgccgcg gccggctgcg tggtgatcga caataccgcg cagttccgct acgacaagga
     6961 catcccgctg gtggtgccgg aagtgaaccc gcatgcgatt gcccagtaca aaaatcgcgg
     7021 catcatcgcc aaccccaact gctcgactat ccagttggtg gtggcgctca aacccataca
     7081 cgatgcggta gggattgtcc gtataaatgt tgcgacctat cagtcggttt ccggcaccgg
     7141 caaagaagct atcgaagaat tggcgcaaca gacccgcgac ctattcaacc acaaagaccc
     7201 ggtggccgag gtgtatccta agcgcatcgc tttcaacgtg ctgccgcata tcgacgcgtt
     7261 ccaggacaac ggctacacca aggaagaaat gaagatggtg tgggagacca acaagatcat
     7321 ggaagacaat tccatcaagg tgaatcccac aactgtgcgg gtgccggtgt tctacgggca
     7381 ctccgaggcg gtgcacctcg aaacccgcaa caaaatcacc gccagggagg cgaccgagct
     7441 tctcaaaaaa gcaccggggg tggtggtgct ggacgagcgt aagccggggg ggtatcccac
     7501 cccggtcact gaagccgcag gccgcgaccc ggtgtacgtg ggacgtatcc gcgaggacat
     7561 ttctcacccg caagggatcg atttatgggt ggtgtcggac aacgtgcgca agggggccgc
     7621 tttaaacagc atccagattg cggagatttt aattgaaaaa tatctggatt aagccataat
     7681 ggtaagaggc aagttgagct tgcatctcta actccatgat gttaatttaa ataactcccc
     7741 taaagactaa agggttaatg agtgcgaaaa tctggattta aagcttggtt gctagtaggc
     7801 acattattta tattgccttg cattgcgcat gccgtcggat tgggcaggct cacggttcat
     7861 tccagcctcg gccaggcgct tgatgccgag gtcgaactcc tcggcgtgca aaaaggggag
     7921 ttggatacgc tggcggcgaa actagcttcg gccgaagcgt tccgtaaggc caatgtcgag
     7981 ttcaatcccg cactgcccgc aatcaaattc agcattgaaa aaaggcctga cggccggcct
     8041 tatctcaaac tcaagacgct gcacccggtc aacgaaccgt tcctcgattt tctgattgaa
     8101 cttaactggg cctcgggccg gctggtgaag gaatatacgg ctctgcttga cccgcctggt
     8161 tatgagccga gacccacgct ggcgccagcc gccccgcccg caccggttgc cctgcccgaa
     8221 gcccaaccct tgcccgagga agcaccgaag cccgaagtgg cgggcgtgcc gccggatgaa
     8281 aaagtcggcg ccgaagcgca agctgcgccg gaagaaggcg aaaccgcggc cccgcaggaa
     8341 gcggccgtcg tcccggaaac acccgcgcca caagccgaag cggtcgagga gaaaaaaacc
     8401 tatggaccga taaagcgcgg tgagacgctg cgcaagatcg ccgaaagtgt aaagcctgtg
     8461 gattattcct tggaacaaat gctggtcggg ctgtttcgct ccaaccacga agccttcatg
     8521 ggcaacaata tgaaccggat gaaaaccggc ccgattttgc gcgtgccgga aaacaccgag
     8581 ctctccacca ttgatccgaa tgatgcggtc aaggaagtgc gcgtacaggc gcgcaactgg
     8641 aatgcctacc ggcaaaagct cgccgaggcc gtgggtcaag ccacacccgc cgctaccgcc
     8701 aagcaaagcg caagcggcga aatttccacc aagattgaag aaaaggccgc acccaaggag
     8761 ccgggcaagg aagtgctgaa actctccaag ggtgaggcgg gcaccggcgg caagcccgct
     8821 gccggtgaca aggcgctgca ggaacggctg cgctcgctcg aagaggaaat tatcgcccgt
     8881 gaaaaggcgg tcaaggaagc caacgagcgc atcacgatgc tggaaaagaa cattcgcgac
     8941 atgcagaagc tgctcgaact caagaaccag aacctcgcag aagtccagaa acaagcgggc
     9001 gcggcgaaac cacaagctgc tcagcctgcg ccgcagccgc aaccggcgcc caaacctgcc
     9061 gaaaccaagc cggctgagaa accggcacag ccgcaacttg ccgcccggcc cgcggccaag
     9121 ccggagcaaa aaccggctca agtcgcacaa cagtcgccgc cgcaggacaa gcccaagcca
     9181 aaaattatca cgccaccgcc ggaggtctcg ttcttagatg atatcctcga caaccccctg
     9241 tatctgggag gaggagcggg cctcataatc gcgttactcg gactcggcta catagccgtg
     9301 agaaaaaaaa aatcaataac gcgttttgaa gacagcgcta tcagcagcga cctgaagacc
     9361 agcacggttt tcggcaatac cgagggaggg acggtcaata ccggcgatag ctcaatcatg
     9421 agcgacttcg ccggcaagat cggcggcacc atcaacaccg atgatgtcga tccgctcgcg
     9481 gaggccgagg tctacatcgc ctacggtcgc gatacgcagg ccgaagaaat cctcaaagag
     9541 gcgctgaaaa aagacccgac acgccagcaa atccatctga agcttctgga aatcctcgcc
     9601 aaccacaaga acgtgtcggc gtttgagtcg attgccagcg atctctatgc ggcatgcggc
     9661 ggcaagggcc tgctctggga caaagccgtt tatctcggct acaagattga tcccaataac
     9721 ccgatgtacg cctccgccaa aggcgcagcc gtggagatga gggaagaaac cgcggcgccg
     9781 cccggcccgc cgcatatgga tttcaacgtc ggggtgccct ctaccggcga gccggttgaa
     9841 gtccaacccg atatcgccat cgacaccgtt gcacccgccg caccggataa tgtggatatc
     9901 agcttcgaaa ttcccacgtc tgaaaccgcg aagaccatac ccgacatacc gtttggaatt
     9961 gcccagcccg ccgatgacga aaccgatttt aatctcgggt cgatcggtgc agaaaccccc
    10021 ttggcagcgg agccgttaaa gccgtcgcaa gaagcggaac ccatgccgga aatgatcagc
    10081 tataattttg ggccttccaa gccggccgag gctccggctc ccgcgccggc cacgtcagaa
    10141 gccgacgcgg ttcacattga cttttccagc atcagcctga acctcgacga gccggcggcc
    10201 gctccggcag cggtgcctgg ggctgccgaa gaagccgcca aggaccagca ttggtacgat
    10261 gtgcagacca agtatgacct ggccaaggcc tacttcgaga tgggcgacaa ggaaggcgca
    10321 cgcgaaatcc tgcaagaagt ggcgcaggaa ggcgacgcca agcagcagga agcggcaaaa
    10381 ggcctgctgg cgcaactctg atccggttta cccgcaacac tttttatcct agtcttggtc
    10441 atgccattac atccggcaac ccgaattggt atttggctgt tgcttgcgat cggcgtgcaa
    10501 ggactccatt ccgtcggact tgccgcgtta agcctgctgt tgctggtcgt ggcctgccgt
    10561 ttccacgggt tcccgcgttt gctcaagcgc acacgctggc tgtttctttc cttgctggtc
    10621 atttatgcca ttgccacgcc gggcgagcta ttgtggccgc aagtcggcgc ggcaagtccg
    10681 accgtcgaag gtttgcgcga aggcgcattg caagcctggc ggctcctatg tttgctcgcg
    10741 gcattggcct ggcttttggc gggcacgccg cgtgctgcgc taatcggcgg cttgtaccgg
    10801 atgctccatc ctttcttcgg taaccacgcc gggcgcattg cggtgaggat ggcgcttacg
    10861 cttgattacg cggaacaggc ggcaggtgtt gaaaactgga aaaatgcatt caacgcggaa
    10921 tttgatggga ctgatacagt atcaacgatc agcctgcgcc tcgagccctt cacctggcgc
    10981 gatgtggtag tgctggcggg cgtgctgctg gcgctcggcg cgcttcgcgc ttggtgaagc
    11041 gcggcaggcg tgggtagcgt gagaatcgcg ctcggattgg aatataacgg cgccgcgttt
    11101 tgcggctggc aaagccagcc cggaggctgc gccgtgcagg acgcggtgga agcggctctc
    11161 tctcaaatcg ccggggagaa attgagtctt accgctgccg ggcgcaccga tgccggcgtg
    11221 catgcgcttt cgcaagtcgc acatttcgac accgccgtag accggccggg cactgcatgg
    11281 gtgcgcggcg tgaatgcgct gttgccgcaa agcgtggcgg cgctgtgggc gcagcccgtc
    11341 gccgacgatt tccacgcgcg tttttcggcc atcgaacgtt gctatcgcta tgtccttctc
    11401 aacaccgcag tgcgcccggc cttgcacgag ggaaaggcgg gctggtttca tcaaccgctc
    11461 gatatggaaa aaatgcgcga agcggcgacg gtcctgctgg gaaagcacga tttcagcgcg
    11521 tttcgagcgg cggaatgccg ggcgcgctcg ccgataaaag aactgcggcg cattgacatc
    11581 atgcgccagc aggattggtt ggtattcgaa ttttgcgccg acgcgttttt gcatcacatg
    11641 gtgcgcaata tcgtaggctg cctagtgtac gtgggcagcg gccgttgtcc cgtccggtgg
    11701 cttgacgaag ttctgcaaag ccgggatcga agcacggcgg cgccgacatt cagtcccgtt
    11761 gggctgtatt tatcggcggt gcgctatgaa gcgaagtggc gcctgcccga ttttaatcgt
    11821 accatgctgc tttttccgta aactgattca tgcctacccg cgtaaaaatc tgcggcatca
    11881 cccgcgtgca agacggcgcg gcggcgggac aattgggcgc gcacgccgtt ggtctcatgt
    11941 tttatgagaa gagcccgcgc tttgtctcgc tcgaaacagc gcgcgaagtg attgcggcgc
    12001 tgccgccgtt tgttgcgcgt gtcggcgttt tcgtcaatcc ggacgaggcg caggtgcgcg
    12061 ccgttctcga cgccttgcgg cttgatttct tgcagtttca tggcgatgaa gcgccggaat
    12121 tttgccgccg gttcggggcg ccttacctca aagcagtgcg agtcaagaag ggtgtggatt
    12181 tgttacaata cgcatcccgc tatcacgaag ccaaggggct gctcctggac gcccacagtg
    12241 aaagcgtgta cggcggcacc ggcgaagcgt tcgattggcg gctcatcccg cggcggctac
    12301 cgctgccggt gatactctcc ggcggcctta acgttgcgaa tgtggccgct gccatcctgc
    12361 aggtcaaacc gtgggcggtg gacgtctcga gcggggtgga atcggcgaaa ggcatcaagg
    12421 acgcggcaaa aatcgccgca tttataaaag aggcgcgtga tgcggatgta tgatcttccc
    12481 gacgagcatg gccattttgg gaaatatggt ggcgtgttcg tcgccgagac gctgattcac
    12541 gctctcgatc aactgcgtga aggctaccag cgctaccgca atgatgccga attccaggcg
    12601 gaattcgccg acgagctcaa gcactacgtc gggcgcccga gtccggttta ccacgccaaa
    12661 cgctggtcgc gcgagcttgg cggcgcgcaa attttgctca agcgcgaaga cctcaaccac
    12721 accggcgcgc ataaaatcaa caacaccgtg ggccaggcgc tgctcgcccg ccgcatgggc
    12781 aaaccgcgcg tgattgccga gaccggcgcc ggcatgcacg gcgtggcgac cgccacggtg
    12841 gcggcgcgct atggcatgga atgcgtggtg tacatgggct ccacagacat aaagcgccag
    12901 gccaccaacg tctaccgcat gaaattgctc ggcgccagcg tggtgccggt ggagtccggc
    12961 tcaaaaacgc tcaaggacgc gctcaacgaa gcgatgcgcg actgggtgac caatgtcgag
    13021 aatacctttt acatcatcgg cactgtcgcg ggtccgcatc cttatccgat gatggtgcgc
    13081 gatttccaat ccatcatcgg gcgcgaggcg aaaacccaga tgcaggaaat gttcgggaga
    13141 cagccagatg cggccatcgc ctgcgtcggt ggcggctcca acgccatggg cttgttttat
    13201 ccttatatcg aggaaaaaag cgtgcgcctc atcggagtgg aagcgggcgg cttggggctg
    13261 cccagcggca agcatgcggc gacgctatgc gccggcaagc ccggggtgct gcacggcaac
    13321 cgcacttatc tcatgcagga tgaaaacggc cagatcatag agacgcactc gatatcggcg
    13381 ggactggatt acccgggcgt cggccccgaa catgcctggc tgaaagacag cggccgcgcc
    13441 gaatacgtta atgtgaccga cgacgaagcg ctgaaggcgt ttcatgacct gtgccacatg
    13501 gaaggcatca ttccggcgct ggaatccgct catgcactgg cctatgcggc gaaaatcgcg
    13561 ccgcagatgg gcagggagca actgctgctg gtgaatcttt ccggacgcgg cgacaaggac
    13621 atgaacaccg tggccaatct ttccggcatc aaattctaaa tgcgtaagga gaccaggagt
    13681 gagcagtaag gcgttaaaat ccagttacac tcgacgtgcc cctcactcct tacaccttac
    13741 ccctcactga atttactatg tcacgcatag cggcaacttt cagcagattg cggcagcaga
    13801 agaaaaaagc gctgattccc ttcatcaccg cgggcgaccc cgagccgggt atgacggtga
    13861 aaatgatgca gaccttgtct gaagggggcg cggatatcat cgaactgggg gtgccatttt
    13921 ccgacccgat ggcggacggg ccgaccatcc aacgctcttc cgaacgggcg ctgaaacacc
    13981 gcatcagcct gaaacagata ctggacatgg tggcggaatt ccgcaaattc aacccgcaca
    14041 cgccggtggt gctgatgggc tatgccaacc cgattgaaac gctggggtat gaaaaatttg
    14101 cgcaagccgc gcaagtcagt ggagtggatg gcgtgctggt ggtggattat ccgccgcaag
    14161 aatgcgagga actggtgaaa catttggagc gttgtggaat cgatccgatt tttctgctgt
    14221 caccgacgac gaccgaagcg cgcattgagg aagtggcaaa gctttcccgc ggctacctgt
    14281 attatgtgtc gctgaaaggg gtcaccggcg ccaggcatct ggacttaagc gatgtggcga
    14341 agaaactgcc gcaaatccgc gcgcgcacca agttgccggt gggcgtgggc tttggcatcc
    14401 gtgatgcgca aactgccaag gcggtggcgg cattagccga tgccgtggtg gtgggaagcc
    14461 gtattgtcga ggaagtggaa aaatcggcaa aagaccaggt gttaggcaag atcgcggtgc
    14521 tgattcagga cttgcgccgc gccatagatc aaccagggtg atgccatgag ctggctgcaa
    14581 aaattactgc caccgaagat caaacgcact ccctccggga ccaagaaaac ggttccggaa
    14641 ggcttgtgga ccaaatgctc caaatgtgaa gcggtacttt accgcagcga gctggagaat
    14701 aacttcaatg tctgccccaa gtgcggaaac caccatcgtc tgaatgcgcg cgaacgcctg
    14761 gaagcgctgc tggatgcgga ggggcgctat gaaatcggcg ctgaagtcat gccggtggac
    14821 acgctgaaat tcaaggacga caagcgctac aatgaacggc tggagcaagc acaagaggaa
    14881 accggagaga gcgaggcgct ggtggtgatg cagggcagca tcaagactgt gccactggtt
    14941 gcggccgcgc acgagttccg tttcatgggc ggctcgatgg
//
LOCUS       DATIOW010000201         2280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1105080, whole genome shotgun sequence.
ACCESSION   DATIOW010000201 DATIOW010000000
VERSION     DATIOW010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105080"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VLL03_06670"
     CDS             complement(<1..526)
                     /locus_tag="VLL03_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HSS47078.1"
                     /translation="MNDIKKVVLAYSGGLDTSVILKWLQDTYKCEVVTFTADIGQGEE
                     LEPARAKAKQLGVKEIFISDLREEFVRDFVFPMFRANAIYEGEYLLGTSIARPLIAKR
                     QIDIARQTKADAVAHGATGKGNDQVRFELTYYALNPDIKVIAPWREWAFKSRSDLIEF
                     AEKHQIPVAKDKRGE"
     gene            complement(630..1550)
                     /gene="argF"
                     /locus_tag="VLL03_06675"
     CDS             complement(630..1550)
                     /gene="argF"
                     /locus_tag="VLL03_06675"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HSS47079.1"
                     /translation="MHTKHFLQFKDFELPEFEYLFERARILKAKRGRGELYQPLHGKT
                     LAMIFEKHSTRTRVSFEAGMNQLGGSAMFLSPRDTQLSRGEPIEDVAQVISRMVDMVM
                     IRTFEQSIIERFASHSRVPVINGLTNEYHPCQILADIFTYLEQRGSIRGITVAWVGDS
                     NNVCNSWLQAAKLFDFNFHVACPPGYELEPERGESYASMHLECFADPHDAVSGADLVT
                     TDVWTSMGYEKETEARKRVFADFRVDEEMMALAKPNVLFMHCLPAHRGEEVAAEVIDG
                     PQSVVWDEAENRLHTQKALMEYLLLGRVKG"
     gene            complement(1578..>2280)
                     /locus_tag="VLL03_06680"
     CDS             complement(1578..>2280)
                     /locus_tag="VLL03_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSS47080.1"
                     /translation="AQVAGHDKNIVAVLVEPIQGEAGIRVPLPDYLQGLRRICDEQGW
                     LLMLDEVQSGIGRTGKWFAHQYSGVKPDVMTLAKGLGSGVPIGACLAAGEAAEVFKPG
                     SHGSTFGGSPLACVAALTTLAVIEEEKLMPHAVKLGEFIRNGLAQELSGVKDVVQIRG
                     AGLMIGIELAQPCGDMVEDALEQGLLINVTAENVIRLLPALVMQRAEAQQLIDSLSGL
                     IRAFSQQAASANAGA"
BASE COUNT          451 a          695 c          632 g          502 t
ORIGIN      
        1 cctcgccgcg cttgtccttg gcgacgggga tctggtgctt ctcggcgaac tcgatcaggt
       61 cgctgcggga cttgaacgcc cattcccgcc aaggcgcgat caccttgatg tccgggttca
      121 gcgcataata ggtgagctcg aagcgtacct ggtcgttgcc cttgccggtg gcgccgtgtg
      181 ccaccgcatc tgccttggtt tgccgggcaa tatcaatttg ccgcttggcg atgagcgggc
      241 gggcgatact ggtgccgagc aggtattcgc cctcgtaaat agcgttcgcc cggaacatcg
      301 gaaacacgaa atcgcgcacg aattcctcgc gcaagtcgct gatgaaaatt tccttcaccc
      361 caagctgttt ggccttggcc cgcgccggct cgagctcttc gccctgtccg atatccgccg
      421 tgaaagtcac cacttcgcac ttataggtat cctgcagcca tttgaggatg accgaggtat
      481 ccagcccgcc tgagtaggcg agcaccactt ttttaatatc gttcatgatt aatccctaaa
      541 aatcttgaac cacggaggac gccaaggacg cagaggaatt caaactcaat aataatcagc
      601 atgtttttcc tccgcgtcct ctgcggtgat cagcctttca ctcttcccag caacaaatat
      661 tccatcaacg ctttctgcgt gtgtaaacgg ttctcggcct cgtcccatac taccgactgt
      721 ggaccatcaa tcacttcggc cgcgacttcc tcgccacggt gggcggggag gcaatgcatg
      781 aacaagacat tgggtttggc cagtgccatc atttcctcat ccacccggaa atcggcaaac
      841 acccgtttgc gtgcctcggt ttccttttcg tagcccatgc tggtccacac gtcggtggtg
      901 accagatcgg cgccgctcac cgcgtcgtgc gggtcggcga agcattcaag gtgcatgctg
      961 gcatagcttt ctccgcgctc gggctcaagc tcgtagcccg gcgggcaggc gacatggaaa
     1021 ttgaagtcga aaagtttcgc cgcttgcagc caggaattgc acacgttgtt ggaatcacca
     1081 acccatgcca cggttatgcc gcgaatcgaa ccgcgctgtt ccagataagt aaaaatgtcg
     1141 gctaggatct ggcaggggtg gtattcgttg gtgagcccgt tgatgaccgg cacccgcgag
     1201 tggctggcaa agcgctcgat gatgctttgc tcgaaagtgc gtatcataac catgtccacc
     1261 atgcgcgaaa tcacctgcgc cacgtcctcg atcggttcgc cgcgtgaaag ctgagtatcg
     1321 cgcggggaaa gaaacatcgc cgaaccgccg agctggttca ttcccgcctc gaaggagacg
     1381 cgagtgcggg ttgagtgttt ttcgaaaatc atcgccagcg tcttgccgtg aagcggctga
     1441 tagagttcgc cgcgcccccg cttggcctta aggatgcgcg cgcgttcgaa cagatactcg
     1501 aactcgggga gctcgaaatc tttgaactgc aaaaaatgtt tggtgtgcat gatgagtctt
     1561 tttttcttgc tcgcacatca ggcgccggca ttggcggaag ccgcttgctg cgaaaaagcc
     1621 ctgatcaggc cgcttaagct atcaatcaat tgctgcgcct ctgcccgctg catcaccagc
     1681 gcgggcagca gacgaatcac gttttccgcg gtgacgttaa tcaaaagccc ctgttccagc
     1741 gcgtcttcca ccatgtcgcc gcaaggctgc gcgagctcga tgcctatcat caaacctgcg
     1801 ccacggattt gcaccacgtc tttgaccccg gatagttctt gtgccaggcc gttgcggatg
     1861 aattcgccga gcttgaccgc gtgaggcatg agtttttctt cctcgatgac ggcaagcgtg
     1921 gtgagtgcgg cgacgcaggc gagcgggctg ccgccgaatg tcgagccgtg gctgccgggt
     1981 ttgaacactt ccgccgcctc tcccgccgcg aggcaggcgc cgataggcac tccggaaccc
     2041 aaacccttgg cgagggtcat cacatcgggc ttgaccccgg aatactggtg cgcaaaccat
     2101 tttccggtgc ggccgatccc gctttgcact tcatccagca tcaaaagcca cccctgctcg
     2161 tcgcagatgc ggcgcagtcc ctggaggtaa tcaggcagcg gcacacggat cccggcttca
     2221 ccctggatcg gctccaccag caccgcgacg atgtttttgt catggcccgc cacctgcgca
//
LOCUS       DATIOW010000202        16319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1713550, whole genome shotgun sequence.
ACCESSION   DATIOW010000202 DATIOW010000000
VERSION     DATIOW010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16319
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1713550"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VLL03_06685"
     CDS             complement(<1..465)
                     /locus_tag="VLL03_06685"
                     /inference="COORDINATES: protein motif:HMM:NF025252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HSS47081.1"
                     /translation="MSIALEARRLLRKNRFGMLATLSKKFSGHPFGSVTPYILDHDAR
                     PVILISTLAEHTKNIENDSRVSLLVHEAGDNVQAEARITVLGNCARMQDQEQVKTRYL
                     RYFPNASGYFDTHDFFFYRIAPIMIRYIGGFGEIHWIPAENFTAPENQLAAQE"
     gene            complement(462..1052)
                     /gene="wrbA"
                     /locus_tag="VLL03_06690"
     CDS             complement(462..1052)
                     /gene="wrbA"
                     /locus_tag="VLL03_06690"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HSS47082.1"
                     /translation="MKDILVLYYSHYGAVKQMAQAVARGIEQVKGVKARIRTVPKVST
                     VCEAVEKDIPDSGAPYAELTDLEECIGLAMGSPTRFGNMAAPLKYFWDGAGSLWMKGT
                     LIGKPAALFTSTASMHGGQETTLLSMMLPLLHHGMVILGIPYSEPELVSTKSGGTPYG
                     ASHIAGMAGDQPITEEERKLCIALGKRLAETALKLA"
     gene            1171..2397
                     /locus_tag="VLL03_06695"
     CDS             1171..2397
                     /locus_tag="VLL03_06695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HSS47083.1"
                     /translation="MPRRVYSGASYLRFVLKRFRQDHCLQIAGSLTFTTLLALVPLIT
                     VSLAILAAFPVFSDFSAELKAFIWNNLMPQTAGKVISVYLKQFTEHAARLTTVGIVFL
                     GVTALMLMLTIDRALNTIWHVTRLRPLVNRLLIYWAVLTVGPILIGASLSLTSWLLSA
                     SLGLTKHMPELGVLLLNVTPVLLSAIAFVLLFVIVPYRYVPVSHAFAGAITSAVAFEF
                     MKRGFALYIAHMPTYKLVYGTFASIPIFLLWIYLSWLVILLGAVIAAALTNWRGETTI
                     QERVPGYRFYYALRVLQVLHHSLKTGEVTSLPKLRKQLQLGVEELEEVLQRLSSASWV
                     RKAAGEGWVLARDPETLTVGDVYRLFVFDPERAHPALDSGLPDAFLHNLFEQGEMKTP
                     LKTFFSRNPNGQTSAV"
     gene            complement(2400..3968)
                     /locus_tag="VLL03_06700"
     CDS             complement(2400..3968)
                     /locus_tag="VLL03_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS47084.1"
                     /translation="MPLKISLPQSGESLKLKTEMRPKQVKTWLEALPLANIMEAAHSV
                     SDALNSLNHVPVKDHLRLKLLELYTPAIDNLVQELQPKYTLAGLPLPEKSRQAATLAH
                     TLFTELAYGYKMVLLERLDKRLIFRGTHLHLIIQRTLAALSKVLCVFYSTYSPTPEGI
                     WSEIHQLAQFALQRNLHDEPVDGGKHSISSTYKQTLLLALANPYRLMQGEVGRVNDYL
                     ATHSNQAKLFPVRPTQGSSGLFLINLESDGPPKSADQEGNINPRSHILLGTDALVSTL
                     HEQIAKLESGRPPKQLNLPDYAKDAAYLNLMRRLLKDWTAVPRRKFKRMANDDTIEIC
                     VGLHATCHFLAETGTEEETLDFIGNRPAKSASAKIAKGQYPVSQWTVTNVSASGLRLK
                     RLSGDNVQIRVGNVIGMRVQGGQQWNIGVVRWVISDEPENFELGVQMLAPSATAITLK
                     PVVSGPREIFRAALLLPEIRPLQQPATIIASPATFRAQLEYLLYENGVVSNVRAMRLV
                     EQTASFDQFQYSVE"
     gene            4237..4770
                     /locus_tag="VLL03_06705"
     CDS             4237..4770
                     /locus_tag="VLL03_06705"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSS47085.1"
                     /translation="MTRLICVIGLMLLSYAAQAEGFVLTDTEGKTHRLSDYQGKWVLV
                     NFWATWCPPCLEEIPGLVSLYEAHRKEVEVIGIAMEYQDPKAVIEFADNLFMSYPVVL
                     GNDKIAAQIGRVNGLPTTFIFSPHGKMVMRHVGAVTRESVEQFMGFKKGGRGEKVFLD
                     RMEGNPLVRLDAAGFSP"
     gene            complement(4744..5349)
                     /locus_tag="VLL03_06710"
     CDS             complement(4744..5349)
                     /locus_tag="VLL03_06710"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008647246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HSS47086.1"
                     /translation="MRKLIGCWLLLFSPLAFAGAQIYEPLSASVRAVMQKAVSDKASP
                     RLAFGSEAEAKAWLDEMSRRLGDRVPDAKTRKDFLTTVHYEATRAGLDPQLVLGLIQV
                     ESRFRKYAVSKAGARGYMQVMPFWVREIGAGEHNLFHLRTNLRYGCTILRYYLDKENG
                     DLFRALGRYNGSLGQSNYPNRVVRAWINQWKYPLRGKPSRV"
     gene            complement(5337..7049)
                     /locus_tag="VLL03_06715"
     CDS             complement(5337..7049)
                     /locus_tag="VLL03_06715"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HSS47087.1"
                     /translation="MRVSQFFLSTLKEAPAEAELASHRLMLRAGLIKRLTSGIYTWMP
                     LGLRVLRKVETIVREEMDRSGAIELLMPAVQPAELWQESKRWEQYGPELLRIKDRHQR
                     DFCFGPTHEEVITDIVRREIRSYRQLPLNFYQIQTKFRDEIRPRFGVMRAREFVMKDA
                     YSFHADKASLDQTYQLMYHTYSRIFTRLGLRFRAVVADTGTIGGTGSHEFHVLADSGE
                     DAIAFCPDSDYAANVELAEALPPTTPRAPGTQKLQKVATPGKKTVEEVSEFLKVSPQK
                     IIKSIMVMTKNNEAYLLLLRGDHALNEAKALKIPALADGFRLASEEEIQSNMHCRSGY
                     IGPANTHVPALLDRSVASMSDFICGANEDGCHFIGANFDRDVSYAKDAIFDLRNVVAG
                     DPSPDGKGKLEICRGVEVGHIFQLRTKYSSAMNCTFVDEKSETQTMEMGCYGIGVSRI
                     VGAAIEQNHDERGIIFPGPMAPFHLAIVPIGKSNEVRAQAEKLHAELSANGIEVLLDD
                     RDERAGVMFADMELIGIPHRLVIGERGLKQGQVEYQGRRDKEPQPIPLRDCAEFIKNR
                     LCAS"
     gene            7257..7721
                     /locus_tag="VLL03_06720"
                     /pseudo
     CDS             7257..7721
                     /locus_tag="VLL03_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747476.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
     gene            7882..8466
                     /locus_tag="VLL03_06725"
     CDS             7882..8466
                     /locus_tag="VLL03_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSS47088.1"
                     /translation="MFRDYVPLRFFPLQAGTSFHQPSQTLPQRVNLDDRAAECMTDLK
                     QVSAAAIEPSATIDTANQTMIECGVRLLLVVDRWRAVVGIITANDILGEKPMRFMQES
                     GIKRHEVLVQDIMTAQHQQEVLSLDDVLHSKVGHVVATLKKSGRQHAVVVDNAARKIR
                     GIFSATQIARQLGVTIQTTEVARTFAEIEAVLSH"
     gene            8614..9033
                     /locus_tag="VLL03_06730"
     CDS             8614..9033
                     /locus_tag="VLL03_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HSS47089.1"
                     /translation="MQLKGSETHESLKAAFAGESMANRRYLYFAAKADVEGQNDVSAV
                     FRSTAEGETGHAHGHLEYLEACGDPATDQPFGPTRQNLKSAIHGETHEYTDMYPGMAK
                     IARQEGFEEIADWFETLAKAERSHANRFQKALDQLAD"
     gene            9102..10430
                     /locus_tag="VLL03_06735"
     CDS             9102..10430
                     /locus_tag="VLL03_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HSS47090.1"
                     /translation="MTTREGGLEAPKRHPIDWRNPKFYEEESVLQELERVFDICHGCR
                     RCVNLCNTFPHLFDLIDGGATGELDGVEKSKLWGVVDQCYLCDMCFMTKCPYVPPHPW
                     NVDFPHLMLRAKAVKYRKGEVKFRDKLLSSTDALGKLAGIPVVVQMVNAVNKTTAARK
                     VMHNVLGIHQERQLPEFTPSTFRKNAAANHGFAVKDGKNTPGKVAVFSTCYVNYNEPG
                     IGHDLLKILEHNEIPAVVVEKEACCGMPKLELGDLDAVEKLKNTNIPPLVKLARQGYA
                     ILTAVPSCTLMFKQELPLLFPDDADVKAVAEAMFDPFEYLVLRQRDGLLNTDFNKPLG
                     KVSYHIPCHLRVQNVGQKTRELLQMIPGTQVNTVERCSGHDGTWGVKTEFYGHSMKIG
                     RPVFKQMAMGGPDYISSDCAIAGRHIAQGMGGTTAQKQHPLTLLRIAYGL"
     gene            10466..11053
                     /locus_tag="VLL03_06740"
     CDS             10466..11053
                     /locus_tag="VLL03_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HSS47091.1"
                     /translation="MPVTRDSLMTLEAYAKMRKEFRAKVMEHKKTRKIHFDEHVTLIF
                     EDELTIRYQIQEMLRVERIFEEEGIQDELQAYNPLIPDGSNWKATMLIEYPDVEERKK
                     MLARLKGIERKVWVKIVGYAPVYAIADEDLERDDGEKTAAVHFLRFELTPQMAQALKN
                     GAGLAMGIDHSVYQAAIENVDPTVRGSLLKDLRLL"
     gene            11098..12312
                     /locus_tag="VLL03_06745"
     CDS             11098..12312
                     /locus_tag="VLL03_06745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSS47092.1"
                     /translation="MFENQVKYWLEQRISALNLPLSICLPDQQLITPAGSPKVTIMLR
                     TPRALLTLANPTLGKLAKAYVEQEFDVRGNLRDVVEIVVGLCDAGAEKGRKKINHAWK
                     WRRHTLSADRRAITHHYDVSNDFYALWLDRWRVYSCAYFKTPEDSLDLAQEQKLDLIC
                     RKLDLQQDERFLDIGCGWGGLILWAAQRYRAKATGITLSRNQFEYVGQKIKELGLQDC
                     CQVMLCDYRELDEREPFDKIASVGMFEHVGRRNLPLYFGKIYRLLKPGGLVMNHGITL
                     TSPGCGELGSDRGKFIEDYVFPGGELVHVSVVMETMSQQKLECLDAENLRAHYAKTLW
                     HWVERLDAKQDQARRIVGEKIFRTWQVYMAGSADAFERGWISIYQLLAGRPLADGRLV
                     YPLTREFVYQTI"
     gene            complement(12309..13427)
                     /gene="proB"
                     /locus_tag="VLL03_06750"
     CDS             complement(12309..13427)
                     /gene="proB"
                     /locus_tag="VLL03_06750"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HSS47093.1"
                     /translation="MREALKNAKRLVIKVGSSLVTNEGQGLDHAALARWAEQIAALKK
                     LGKEVLLVSSGAIAEGMQRLDWRKRPHSMHELQAAAAVGQMGLIQAYESCFRKFGLHT
                     AQILLTHEDLSDRKRYLNARSTLRSLLALNVIPVINENDTVVTDEIRFGDNDTLGALV
                     TNLIEADALIILTDQAGLYTADPRQNPRATLVTEACAGDENLEKMAGSSGTHIGSGGM
                     LTKVFAAKRAARSGAHTVIAPGREANVLLRLAQGEALGSLLFAETVTLAARKQWLADH
                     LQVRGKLMLDAGAVQALCGSGKSLLPIGVYDLSGDFERGEVVTCVDPHGREIARGLVN
                     YNAQETRRILRLPSQEIESVLGYVDEPELIHRDNLVLL"
     gene            complement(13431..14456)
                     /gene="obgE"
                     /locus_tag="VLL03_06755"
     CDS             complement(13431..14456)
                     /gene="obgE"
                     /locus_tag="VLL03_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HSS47094.1"
                     /translation="MKFIDEAIIQVSAGKGGDGVASFRREKFIPRGGPDGGDGGRGGS
                     VYAIADRNINTLIDYRYARIHRAKNGENGRGSDCYGKSAEDVMLRMPVGTVITDNLTG
                     ELIADLDQHGQKAPLAKGGGGGLGNLHFKSSTNRAPRKCTPGQPGEVCELKLELKLLA
                     DVGLLGLPNAGKSTFIRAVSAARPKVADYPFTTLHPHLGMVRVGESRSFVIADIPGLI
                     EGAAEGAGLGHQFLKHLARTRLLLHIVDIASFDEIADAAREARAIIKELKKYDEALYR
                     KPRWLVLNKLDLLDVSEREKKSRRFLRDLGWKGKSFIISALTSEGCKELTFAVMQYLE
                     QTYEAEE"
     gene            complement(14557..14814)
                     /gene="rpmA"
                     /locus_tag="VLL03_06760"
     CDS             complement(14557..14814)
                     /gene="rpmA"
                     /locus_tag="VLL03_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HSS47095.1"
                     /translation="MAHKKAGGSSRNGRDSQSKRLGVKCFGGERVPAGSIIVRQRGTQ
                     VHPGDNVGIGKDHTLCAKIAGKVQFKVKGPEQHKTVSVIPV"
     gene            complement(14827..15138)
                     /gene="rplU"
                     /locus_tag="VLL03_06765"
     CDS             complement(14827..15138)
                     /gene="rplU"
                     /locus_tag="VLL03_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HSS47096.1"
                     /translation="MYAVIKTGGKQYRVASGEKLKIEQIPQAVGTEIVLDQVLLVANG
                     DKIAAGNPLVAGAKVKATVVAQGRGDKVRIFKMRRRKMYRRTQGHRQNFTEIRIDTIS
                     A"
     gene            15318..16298
                     /gene="ispB"
                     /locus_tag="VLL03_06770"
     CDS             15318..16298
                     /gene="ispB"
                     /locus_tag="VLL03_06770"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HSS47097.1"
                     /translation="MGTSLSIEAIRDAIEPEMRVVDQVIRQRLHSDVALIRQVAEYII
                     NSGGKRLRPALVILSAGAFGYRGNHHYELAAVVEFIHTATLLHDDVVDGSALRRGSPT
                     VNALFGNAASVLVGDFVYSRAFQMMVEVDSMRVMRVLADATNVIAEGEVLQLLNCHNA
                     NIDEQRYLQVIRCKTAKLFEAATRLGAILGHADSAEEEAMASYGMHLGIAFQLIDDVL
                     DYSGDLSQTGKNIGDDLAEGKPTLPLIYAMKHGNPEQAALIRKAVEQGGREEFATVLD
                     TIHKTRALEYARTQAKTEAQAACAACAMLPRSNYRDSLLQLADFAVSRSY"
BASE COUNT         3357 a         4532 c         4743 g         3687 t
ORIGIN      
        1 ttcctgtgcc gcaagctggt tttcgggggc ggtgaaattt tccgctggaa tccagtggat
       61 ttcgccgaaa ccgccaatgt aacggatcat gatgggagca atgcgataga aaaagaaatc
      121 gtgagtgtcg aagtagcctg acgcatttgg aaaatagcgc aggtagcgtg ttttcacttg
      181 ttcctggtcc tgcatgcgcg cgcagttgcc gagcaccgta atccgcgctt ccgcttgcac
      241 gttgtcgccc gcttcatgaa cgagcaggct cacacggctg tcgttttcga tgttcttggt
      301 gtgctctgcg agcgtgctga tgagaatgac tggccgcgcg tcgtggtcga ggatgtaggg
      361 ggtaaccgaa ccaaacggat ggccgctgaa tttctttgag agagtcgcca gcatgccgaa
      421 gcggttttta cggagcaaac gccgcgcctc taaagctata ctcatgcgag cttgagggcg
      481 gtctcggcga gccgcttgcc gagcgcgatg cagagtttgc gttcttcttc ggtaatgggt
      541 tggtcgcccg ccattcctgc gatgtggctc gcgccatagg gagtgccgcc gcttttggtg
      601 ctgacaagct ccggctcgga gtagggaatg cccaggatca ccatgccgtg atgcaaaagt
      661 ggcagcatca tcgaaagcag cgtcgtttcc tgtccgccgt gcatggaggc cgtggaagtg
      721 aacagcgcgg cgggttttcc gatcaacgtg cctttcatcc acaagcttcc ggcgccgtcc
      781 cagaagtatt tgagcggcgc cgccatattg ccaaagcggg tcgggctgcc catggcgagg
      841 ccgatgcatt cttccaagtc cgtaagctcg gcgtaagggg cgccggaatc gggaatatct
      901 ttttccaccg cttcacacac agtggatact ttgggcacgg tgcggatgcg tgctttcacg
      961 ccttttacct gctcaatgcc gcgcgccacg gcctgcgcca tttgtttcac cgcgccatag
     1021 tggctgtaat agaggaccag aatatctttc atgacgtagg aaacctctga ttaagtcccc
     1081 cgcgtgacgt gtaggactta atcagagatt ccttagtttt ttgctgaatt cgccggtatt
     1141 atatagcaac ccgaacaacc atccttgttc atgcccagaa gagtctattc cggcgccagc
     1201 tatttgcgct ttgtcctgaa gcggttcagg caagaccatt gcctgcaaat agcggggagc
     1261 ctcaccttca ccaccttgct cgcgctggtg ccgctgatta ccgtctcact cgcgatatta
     1321 gcggcgttcc cggtgttctc ggacttttcc gccgagctca aggctttcat ctggaacaat
     1381 ctgatgccgc agacggcggg caaggtcatc agcgtttacc tgaagcagtt caccgagcat
     1441 gccgcgcgcc ttactaccgt ggggattgtg ttcctagggg tgacggccct gatgctgatg
     1501 cttaccatag accgcgcgct aaacaccatc tggcacgtta cccgtctgcg gccgctcgtc
     1561 aatcgcctgt tgatttattg ggcggtgctg acggtagggc ctatattgat aggcgcaagc
     1621 ttgtcgctta cctcctggct tctcagcgct tccctgggtt tgaccaagca catgcctgaa
     1681 ctaggcgtgc tcctgctcaa tgttacgccg gtgctgctct ccgcaatcgc gtttgtgctg
     1741 ctgtttgtga tcgtccctta tcggtatgtc cccgtttcac atgcgttcgc cggtgcaatc
     1801 acttcggcag tggcgtttga attcatgaag cgtggttttg cgctgtatat cgcgcatatg
     1861 ccgacctata aactggtata cggcactttt gccagcatcc cgattttctt gctgtggatt
     1921 tatctctcct ggctggtgat tctgttgggg gcggtgattg ccgcagcgct cacgaactgg
     1981 cgcggagaaa ccaccatcca ggaacgggtg cccgggtatc ggttttacta cgcgctgcgg
     2041 gttttacagg tgctgcacca ctcgcttaaa accggcgaag tgacgagcct gccgaaattg
     2101 cgcaagcaat tgcaactggg ggttgaagag ctggaagaag tactgcagcg gcttagcagc
     2161 gccagctggg tgcgcaaggc agcaggcgag ggttgggttt tagcgcgcga ccccgaaacg
     2221 cttaccgttg gcgacgtcta ccgcttgttc gtgtttgatc cggagcgcgc ccatcccgcg
     2281 ctagacagcg gcctgcccga tgcttttttg cacaacttgt ttgagcaggg agagatgaaa
     2341 acgccgctga aaactttctt cagccgcaac ccaaacgggc agacgtctgc agtttgaatt
     2401 tattcaacgc tgtactggaa ttgatcgaaa ctcgccgttt gttcgaccag gcgcatggcg
     2461 cgcacgttgc tgaccacgcc gttctcataa agcagatatt ccagttgggc gcggaaggtg
     2521 gcaggcgaag cgatgatggt ggccggctgc tgcaacggac ggatctccgg cagcagcagc
     2581 gccgcccgaa agatctccct gggaccggag accaccggtt tgagggtaat cgccgtggct
     2641 gacggcgcca gcatttgcac ccctagttcg aagttctcgg gttcatcgct gattacccac
     2701 cgcaccacgc cgatgttcca ctgctggccg ccctgcaccc gcatgccgat gacattgcca
     2761 acgcggattt gcacgttatc gcctgagagg cgcttcaatc tcagcccgct ggcgcttacg
     2821 ttggtgaccg tccattggct tactgggtat tggcctttgg ctatcttggc tgaagcggat
     2881 ttggcgggtc ggttgccgat aaaatccagg gtctcttctt ccgtgcctgt ctcggcaagg
     2941 aaatggcagg tggcgtgcag gccgacgcag atttctatgg tatcgtcgtt ggccatgcgc
     3001 ttgaacttgc gccgaggcac agccgtccag tctttgagca gccgccgcat cagattgagg
     3061 tacgccgcgt ccttggcgta atccggcaga ttaagctgct tcggcggccg gcctgattcg
     3121 agtttggcga tttgctcgtg taaggtggag accagcgcgt cggtgccgag caggatgtgg
     3181 ctgcgcggat tgatattgcc ctcctgatcg gcggatttgg gcggaccgtc gctttccaga
     3241 ttgatgagaa ataaaccgga gctgccttgg gtcggccgca cggggaaaag cttcgcctgg
     3301 ttgctgtggg tggcgaggta atcgttaacc cggccgactt cgccctgcat cagccggtag
     3361 ggattggcga gcgccaggag cagggtttgc ttgtaagtgc tgctgatgct gtgcttgccg
     3421 ccgtccaccg gttcatcatg cagattgcgc tgcagggcga actgggccaa ctggtggatt
     3481 tccgaccaga tgccttccgg agtcggcgaa taggtactgt aaaaaacgca caagaccttg
     3541 cttaaagcgg caagcgtgcg ctggatgatc aagtgcaggt gggtgccgcg gaagatcagg
     3601 cgcttgtcca agcgctcgag caaaaccatt ttgtagccat acgccaactc ggtgaacagg
     3661 gtgtgcgcca gagttgccgc ctggcggctc ttttccggca gcggcaagcc cgccagtgtg
     3721 tatttgggct ggagttcctg caccagattg tcaatcgccg gcgtgtaaag ctcgagcagc
     3781 ttgagccgca agtggtcctt gaccggcaca tggttcagcg aattcagcgc gtcggaaacg
     3841 ctgtgcgctg cttccatgat attggcaaga ggcaaagctt ccagccaggt ttttacctgt
     3901 ttggggcgca tttccgtctt gagcttgagg ctttcacccg actggggcaa actgattttg
     3961 agtggcatag gttattatcg ttagttgata gtatctctga gcttaaatgc cccgggcgcc
     4021 ggaggtaaaa tacaggctgg ccgttatatg ggcggccggt ttcggcattt ttccttcatt
     4081 ttagtatgat ttgcatggcg ccgataggcg tgtcggcccc gaaaatgtaa caggacagag
     4141 catttccccg cccgcttgtt gcaaagcccg ctatccggga acttcccgta tgccccatca
     4201 gtctttgtct catcgtcagt ttagccggat tttgacatga cccgcttgat ttgtgtcatt
     4261 ggattgatgt tgctgtcata cgcggcccag gcggagggct ttgttttgac agacaccgaa
     4321 ggtaaaactc accgcctcag cgattatcag ggcaaatggg tgctggtgaa tttctgggcg
     4381 acctggtgcc cgccgtgtct cgaggaaatt cccggcctgg tgtcgctgta tgaagcgcac
     4441 cgcaaagagg tcgaagtaat cggcattgcc atggaatacc aggacccgaa ggcggtcatc
     4501 gagtttgccg acaacctgtt catgtcctat ccggtggtgc tcggcaacga caaaatcgcc
     4561 gcgcaaatcg gcagggtgaa cggcctgccc actaccttca ttttcagccc tcatggcaag
     4621 atggtgatgc ggcatgtcgg cgcggtcacc cgcgagagcg tggaacagtt catggggttc
     4681 aagaaaggcg gtcggggaga gaaagtcttt ttggaccgaa tggaagggaa cccgctggtc
     4741 cgcttagacg cggctgggtt ttccccgtaa cggatatttc cactggttga tccaggcccg
     4801 taccacccgg ttcggataat tcgattgccc caagctgccg ttgtagcgtc ccagagcgcg
     4861 gaacaggtcg ccgttttcct tgtcgaggta atagcgcaag atggtgcagc cgtagcgtaa
     4921 gtttgtacgc aggtgaaaca gattgtgctc tccggcgccg atttccctga cccagaacgg
     4981 cattacctgc atgtagccgc gcgcgccggc tttggacacg gcgtatttcc tgaagcggct
     5041 ttccacctgg atcaagccca ggaccagttg tgggtcgagc ccggcgcggg ttgcttcgta
     5101 atgcacggtg gtaagaaaat ccttgcgcgt tttggcgtcg ggaacgcggt cgccgagacg
     5161 ccgcgacatt tcatccagcc acgccttggc ttcggcttct gagccgaagg cgagtcgggg
     5221 gcttgccttg tcgctcaccg ctttttgcat caccgcgcgt acgctggcgg aaagcggttc
     5281 gtagatttgc gcgcccgcga aagccaacgg gctgaacagc agcagccaac agccgatcag
     5341 cttgcgcata atctgttttt aataaactcc gcgcaatccc ggagagggat cggctgcggt
     5401 tccttgtcgc ggcgaccctg atactcgacc tggccttgct tgagcccgcg ctcgccgatc
     5461 accaggcggt gcgggatgcc gatgagctcc atgtcggcga acatcactcc cgcgcgctcg
     5521 tcgcggtcgt cgagcagcac ctcgatgccg tttgccgaga gctcagcgtg aagtttttcc
     5581 gcttgggctc gcacttcgtt gcttttgcca atcggcacga tggcgagatg gaagggcgcc
     5641 atcgggcccg ggaaaatgat gccgcgttca tcgtggttct gctcgatcgc cgcgccgaca
     5701 atgcgcgaga caccgatgcc gtagcaaccc atttccatcg tctgcgtttc gcttttctcg
     5761 tcaacaaaag tgcagttcat ggcggaggaa tacttggtgc gcaattgaaa gatatgccca
     5821 acctcgacgc cgcggcaaat ttccagtttc cccttgccgt cgggactggg gtcgccggcg
     5881 accacattgc ggagatcaaa aattgcgtct ttagcatatg aaacgtcgcg gtcaaagttg
     5941 gcaccaatga agtgacaacc gtcttcgttt gctccacaaa taaaatcact cattgaagca
     6001 acactccgat ctaagagtgc aggcacgtga gtatttgcgg gacctatgta tcctgacctg
     6061 caatgcatgt tgctctgtat ttcttcttcc gaggccaggc ggaacccatc tgccaaggcc
     6121 ggtattttta atgcttttgc ctcattaagc gcgtgatccc ctcgcaaaag caaaagatag
     6181 gcttcattat ttttcgtcat caccataatt gatttaatga ttttttgtgg agaaactttg
     6241 aggaattcac taacttcttc gacagttttc ttcccgggtg tggctacttt ttgtaatttt
     6301 tgggttcccg gggcacgcgg tgtggtaggt ggtagagctt cggcgagctc cacgtttgcg
     6361 gcgtaatcgg aatccgggca gaacgcaatc gcatcctcgc ccgaatccgc aagcacgtga
     6421 aattcatgtg agccggtacc gccgatcgtt ccggtatccg ccaccaccgc gcggaacctc
     6481 aaacccagcc tggtgaaaat ccgtgagtaa gtgtggtaca tgagctggta agtttgatcc
     6541 aggctcgcct tgtcggcgtg gaacgagtag gcgtccttca tcacaaactc gcgcgcgcgc
     6601 atcacgccga aacgcggacg gatttcgtcg cggaacttgg tctggatctg atagaagttg
     6661 aggggaagct gccggtagct tcggatttcg cgccgcacga tatcggtgat gacttcttcg
     6721 tgcgtcgggc caaaacagaa gtcgcgctgg tgacggtctt tgatgcgcaa gagctccggc
     6781 ccgtattgct cccagcgctt tgattcctgc cacaactccg ctggttgcac tgcaggcatc
     6841 aacaattcaa tcgcgccgct tctgtccatt tcctcgcgca cgatggtttc cactttgcgc
     6901 agcacgcgca agcccagcgg catccaggtg taaatgccgc tggtcaggcg cttgatcagt
     6961 ccggcgcgca acatcaagcg gtggctggca agctccgcct cggcaggcgc ttctttcagc
     7021 gtggacagaa aaaattgcga gacgcgcatg aacgttcagg ctccaattca aaatttccgt
     7081 attttacatt ttgcaagctg ctgctgtcag atttttgcta tgtctaaggc acagggtggg
     7141 tatcgtgcag ccccgaaccg ttgcacaaac agtggtttca aagggccaga cgtctttcat
     7201 tctcctctga aatatgaaaa aattgacctg ttataaagaa ttgacgggtg gtggatatga
     7261 ttgacagaga gggttatcgc gccaacgtcg gcatcattct tctgaattcc aggaacgaag
     7321 tcttctgggg taagcgcgta aaagagcatt cgtggcaatt cccgcaaggc ggcatcaagt
     7381 cgggggaaac ccctgagcag gcgatgtacc gcgagctgat ggaagaagtg ggcttgggcc
     7441 cgcagcatgt gaaaatcatc ggccgtaccc gcaactggtt gcgctatgac gtgccgtatc
     7501 aatgggtcag gcgtgaatgg cggggaagtt acaaggggca aaagcaaatc tggtatctgc
     7561 ttaggctggt cggccgggat accgatgtga agctgcgcgc gaccgaccat ccggagttcg
     7621 acgcctggtg ttggcagagt tattgggtgc cgctggaatc ggtgattgaa ttcaagcgcg
     7681 acgtttatcg ccaggcgttg agcgagcttt cgcgttttat caactcggga tttgccggcc
     7741 gcggcgagaa taaaccccga ccggccgagc gctagcgtct gcacccggac tgtgctatcg
     7801 ggttcgcgag tttctgtctg ccgctctttt tcgggaggtg ctataacaaa aacatgaaga
     7861 cccactgtaa tggaggtgcg catgttcaga gattacgttc cgcttcggtt ttttcccctg
     7921 caggccggaa ccagttttca tcagccctcg caaacgctgc cgcagcgggt gaatctggac
     7981 gaccgggcgg ccgagtgcat gaccgacctc aagcaagtgt ccgccgcggc cattgaaccg
     8041 tctgccacaa ttgataccgc caaccagacc atgatcgaat gcggggtaag gctgttactg
     8101 gtcgtggacc gctggcgtgc ggtggtgggc atcatcaccg cgaacgacat cctcggcgaa
     8161 aaaccgatgc ggttcatgca ggaaagcggc atcaagcgcc acgaggtgct ggtgcaggac
     8221 atcatgacgg cacaacacca gcaggaagtg ctgagcctgg acgatgtgct gcactcgaag
     8281 gtcgggcacg tggtcgccac gctcaaaaaa agcggcaggc agcatgccgt ggtggtggat
     8341 aatgcggcgc gcaagatacg cggcatcttt tccgccacgc agatcgcgcg gcaattgggc
     8401 gtcaccattc agactaccga ggtcgcccgc acctttgccg aaatcgaggc ggtgttaagc
     8461 cactgatttc ttgcctgagg atggaattga catgggttta agcgttgatt atacttagtt
     8521 catagattag attaagtcta aactattata tttgattgaa acgctttttc ttaatagaaa
     8581 tactttgata ttagtaactt ataggagaca tagatgcagc tcaaaggatc tgaaacccat
     8641 gaaagcttga aagcggcatt tgccggtgaa tccatggcaa accgccgtta cctctacttc
     8701 gcggcgaaag cggatgtgga gggacaaaac gacgtctcgg cagtgttccg ttctaccgca
     8761 gaaggcgaaa ccggccatgc ccacggtcat ctcgagtacc tggaggcttg cggcgacccg
     8821 gcaaccgatc agcctttcgg accgactcgt caaaacctga aatccgcgat ccatggcgaa
     8881 acccacgaat acactgacat gtatccagga atggcgaaaa tcgcccggca ggaggggttc
     8941 gaagaaatcg cggactggtt tgaaacgctg gctaaagccg agcgctcaca cgccaatcgt
     9001 ttccaaaaag ctctcgacca actggccgac taagccttac caagacgtta ggcgtgaggg
     9061 gcgatccctc gcgccttgtt gtttaaaaac ttattgcatc catgaccaca cgcgaaggcg
     9121 gtctcgaggc acccaagcgc caccctatcg actggcgcaa tccgaaattt tacgaagaag
     9181 aatccgttct ccaggaactg gagcgggtat tcgacatctg ccatggctgc cgtcgttgcg
     9241 tgaatctttg caacaccttt ccgcacttgt ttgatctcat tgacggaggc gccaccggcg
     9301 aactcgacgg cgtggagaaa agcaaattat ggggcgtggt cgaccagtgc tatctgtgcg
     9361 acatgtgctt catgaccaag tgcccgtacg ttccgccgca tccatggaac gtggattttc
     9421 cgcatttgat gctgcgcgcc aaggcggtga aatacagaaa gggcgaggtc aaattccgcg
     9481 ataaactgct ttccagcacc gatgctttgg gcaagctcgc cggcattccg gtggtggtgc
     9541 aaatggtgaa tgcggtgaat aaaacgaccg ccgcccgcaa ggtgatgcac aatgtattgg
     9601 gtattcatca ggagcggcaa ttgccggaat tcacccccag cacgttccgc aagaatgcgg
     9661 cggcaaacca cggctttgca gtaaaggacg gcaagaatac acccggcaaa gtggcggttt
     9721 tttccacttg ttatgtgaac tacaacgaac ccggcatcgg ccacgatttg ctgaaaatcc
     9781 tcgagcacaa cgagattccc gcagtggttg tggaaaaaga agcttgttgc ggcatgccca
     9841 agctcgaact cggcgatctt gacgcagtgg aaaaactcaa gaacaccaac atcccgccgc
     9901 tggtcaaatt agccaggcag ggttatgcca ttttgaccgc ggtaccgtcg tgtacgttga
     9961 tgttcaaaca agaactaccg ttgttgtttc ccgatgacgc cgatgtgaaa gcagtggccg
    10021 aagccatgtt cgatccgttc gaatacctgg tgctgcgcca gcgtgatggc ctgctcaaca
    10081 ccgatttcaa caagccgctg ggcaaagttt cttaccatat tccctgccac ctgcgggtgc
    10141 agaacgtggg gcagaaaacc cgcgagcttt tacaaatgat cccgggcacg caggtcaata
    10201 ccgtggaacg ctgttccggc cacgacggca cctggggcgt gaagaccgag ttctacggcc
    10261 actccatgaa aatcggcagg ccggtattca aacagatggc gatgggcggg ccggattaca
    10321 tcagctccga ttgcgccatc gccggccgcc acatcgcgca aggcatgggc gggacgacgg
    10381 cgcagaagca gcatccgttg accctgttgc gcatcgctta tggattatga gaatggatca
    10441 agtcgaaacc aggaaagaaa tttgcatgcc cgttacccgt gacagtttaa tgacgcttga
    10501 agcctacgcc aaaatgcgca aggagttccg cgccaaggtg atggagcaca agaaaacccg
    10561 caaaattcac ttcgacgaac atgtcacgct gatttttgaa gacgagctca ccatccgcta
    10621 tcaaatccag gaaatgctgc gcgtggagcg tattttcgag gaagagggca tacaggatga
    10681 gttgcaagcc tataacccgc tgattcccga tggcagcaac tggaaagcga ccatgcttat
    10741 cgaatacccg gatgttgagg agcgcaagaa aatgctagcg cgcctgaaag gcattgagcg
    10801 caaagtgtgg gtgaaaatcg tgggatatgc ccccgtctat gcgattgccg atgaggacct
    10861 ggagcgcgac gacggtgaaa aaaccgccgc ggtgcatttt ctgcgcttcg aacttacgcc
    10921 gcaaatggcg caggcgctga agaacggagc ggggctggcc atgggcattg atcattctgt
    10981 ttatcaagca gccatcgaaa acgtcgaccc cacggtgcgc ggctcgctcc tcaaggactt
    11041 gcgcctgttg taagaggtgg taagctagag tattcccctg tcccgcgcgc tgccccaatg
    11101 tttgaaaatc aagttaagta ttggctggaa caacgtattt ccgcgcttaa cctgccatta
    11161 agcatttgcc ttcccgacca gcagctcatt acgcctgccg gttcgcccaa ggtcacaata
    11221 atgctgcgca cgccgcgcgc cttgctcacc ctggcaaatc ccaccctcgg caagctcgcc
    11281 aaggcctatg tggagcagga gttcgatgtc cgcggcaact tgcgcgatgt cgtggaaatc
    11341 gtcgtcgggc tttgtgatgc tggcgccgag aagggcagaa aaaaaatcaa ccatgcctgg
    11401 aaatggcggc gccataccct cagcgccgac cgcagggcaa tcactcatca ctacgatgtg
    11461 tcgaatgatt tttacgcctt atggctggat cgctggcgcg tctattcctg cgcctacttc
    11521 aaaactcccg aggacagcct cgatctggct caggagcaaa agctcgattt gatttgccgc
    11581 aagctcgatt tgcagcaaga cgagcgcttt ctcgatatcg gctgcggctg gggcggcctg
    11641 attctgtggg cggcgcagcg ctatcgcgcc aaggccaccg gcattacctt aagccgtaac
    11701 cagttcgaat acgtcggaca aaaaatcaag gaactgggtt tgcaagattg ctgccaagtg
    11761 atgttgtgcg attaccgcga gctcgacgag cgcgaaccgt tcgacaagat tgccagcgtg
    11821 ggcatgttcg agcatgtcgg caggcgcaat cttccgcttt atttcggcaa gatctaccgc
    11881 ctgctcaagc ccggcgggct ggtgatgaat cacggcatca cgctcacctc ccccggctgc
    11941 ggggaactgg gcagcgatag gggaaagttc atcgaagact atgtgtttcc cggcggagag
    12001 ctggtgcatg tgtcggtggt gatggagacg atgtcgcagc agaagctgga atgcctcgat
    12061 gccgagaatt tgcgcgcgca ttatgcgaaa acgctgtggc actgggtgga gcgcctggac
    12121 gcgaagcagg accaggcgcg gagaattgtc ggagaaaaaa tcttccgcac ctggcaggtt
    12181 tacatggcgg gatcggcgga cgccttcgag cgcggctgga tttccattta ccagctgctc
    12241 gcgggcaggc cgctggcgga tggcaggctg gtttatcctt tgacccgtga gtttgtttac
    12301 cagacgatct agagcagaac caagttgtcc ctgtgaatga gctcgggctc gtccacataa
    12361 cccagcaccg attcgatttc ctggctgggc agccgcagga tgcgccgtgt ttcttgggca
    12421 ttgtaattga ccaggccgcg cgcgatttcc cggccatgcg ggtccacgca agtcaccacc
    12481 tcgccgcgct cgaagtcccc gctcaaatcg tacacgccta tcggcagcag gcttttacca
    12541 ctcccgcata aggcttgcac ggcgccggca tcgagcatga gtttgccgcg cacctgcaga
    12601 tgatcggcaa gccactgttt gcgcgcagcc aaagtcaccg tttcggcgaa aagcaggctg
    12661 cccagcgctt cgccttgcgc gagccgcagc agcacattgg cctcgcggcc gggagcaatc
    12721 acggtatgcg cgccgctgcg cgccgcgcgt ttggcggcga acactttggt aagcatgccg
    12781 ccgctgccaa tgtgcgtgcc gctgcttcct gccatttttt cgagattctc gtcgccggcg
    12841 cacgcttcgg tgacgagggt tgctcggggg ttctggcgcg gatcggccgt gtaaagaccc
    12901 gcctgatcgg taaggatgat aagtgcatca gcttcgatga gattggtcac cagcgcgccc
    12961 agcgtgtcgt tgtccccgaa gcggatttcg tcggtcacca ccgtgtcgtt ttcattgatg
    13021 acgggaatga cgttgagcgc cagcagcgag cgcagggtgg aacgggcgtt gagatagcgc
    13081 ttgcggtccg acaggtcctc atgggtcaga agaatttgcg cggtatgcag gccgaatttg
    13141 cggaaacagc tctcgtaggc ctgaatcaac cccatctgcc ccactgcggc ggcggcctgc
    13201 agctcatgca tgctgtgcgg gcgctttcgc cagtcgagcc gttgcatccc ttcggcaatc
    13261 gcaccggagg aaaccagcag tacttccttg ccgagttttt tcaatgcagc gatctgttcc
    13321 gcccagcgcg ccagcgcggc gtgatccaga ccttgcccct cattggtgac gaggctgctg
    13381 ccgactttga tcaccagtcg tttggcgttt tttaaggctt cacgcatgct ttactcttcc
    13441 gcttcgtagg tttgttccag atattgcatg acggcaaaag tcagttcttt gcagccttcg
    13501 ctggtgagcg ctgaaataat aaagcttttt cctttccagc ccaaatcgcg caggaacctg
    13561 cggctttttt tttcacgttc ggaaacatcc agcaaatcca gcttgttcag caccagccag
    13621 cgcggcttgc ggtaaagcgc ctcatcgtat tttttgagct ccttgatgat ggcgcgcgct
    13681 tcccgcgccg catcggctat ttcgtcgaaa gacgcgatgt ccacgatgtg cagaagcagg
    13741 cgcgtgcgcg cgaggtgctt gaggaattga tggcccagcc ccgcgccttc ggccgcacct
    13801 tcgatcagcc cgggaatgtc ggcgattacg aagctgcgcg attcgcctac gcgcaccatc
    13861 cccagatgcg gatgcaaagt ggtgaaagga taatccgcga ccttggggcg tgccgccgac
    13921 acggcgcgga tgaaagtcga cttaccggcg ttgggcaggc cgagcaaacc gacgtcggca
    13981 agcagcttga gttcgagctt aagctcacac acttcccccg gctggcccgg agtgcacttg
    14041 cgcggcgcgc ggttggtgct ggatttgaag tgcagattgc ccaggccgcc gccgccgcct
    14101 ttggcgagtg gagctttttg cccgtgctga tcgaggtcgg cgatgagttc gccggtcagg
    14161 ttgtcggtga tgaccgtacc caccggcatg cgcaacatca cgtcctccgc gcttttgccg
    14221 tagcaatccg aaccgcgccc attctcgcca tttttggcgc ggtgaatgcg cgcgtagcgg
    14281 taatcaatca gcgtattgat gttgcggtcg gcaatcgcgt aaacgctgcc gccgcgtccg
    14341 ccgtcgccgc catccggtcc gccgcgcggg atgaattttt cgcgcctaaa gctggccacg
    14401 ccatcgccgc ctttgccggc gctgacttgt atgatggctt catcaataaa tttcattgtg
    14461 tctgctttcg tgcccgcttc ggtggaggct cacttccgct aggccagaaa taaaaaagcc
    14521 ctatcgtccg atagggctgt gggaatgaca cgacccttag accgggatca cgcttacggt
    14581 tttgtgctgc tccgggcctt tgaccttgaa ttgaactttt cccgcaattt tggcgcacag
    14641 ggtgtggtcc ttgccgatgc cgacgttgtc gccgggatga acctgggtgc cgcgctggcg
    14701 cacgataatg ctgcccgctg gcacccgttc gccgccaaaa catttcacgc cgaggcgctt
    14761 ggactgggaa tcgcggccgt tgcgggaact gccacccgct tttttatgtg ccatattgga
    14821 tcttccttat gccgaaatcg tatcgatgcg gatttcggtg aaattctggc gatggccctg
    14881 cgtcctgcgg tacatcttgc ggcggcgcat tttgaaaatc cgcaccttgt cgccccgccc
    14941 ttgggcaacc acagtcgcct tgactttggc gcccgccacc agcgggtttc cggcggctat
    15001 tttgtcgcca ttggccacca acagcacctg gtcgagcacg atttcagtgc cgacggcctg
    15061 gggtatttgt tcaatcttga gtttttcgcc ggaagccacg cgatactgct tgcccccggt
    15121 tttgatgacc gcgtacatat ttgctccaga tgccgcttct ttgcaaacgt gtaattttac
    15181 ataaagcctg ctcaccagtc aaaatcaagt ctggctggtt gctccggcgt tgcggtcccg
    15241 gcggcggccg agttcccgcc ccctaaaaat gccacgttct gctatcctga gagttcattc
    15301 ttgcgacgct actcatcttg ggcacatcct tgtcaatcga agccatccgc gacgccatcg
    15361 agccggaaat gcgcgtcgtg gaccaggtca tccgccagcg cctgcattcc gatgtggcgc
    15421 tgattcgcca ggtggccgag tacatcatca acagcggagg gaaacgcctg cgcccggcac
    15481 tggtgattct ttccgccggt gccttcggtt accgcggcaa ccatcattac gagctcgcag
    15541 cggtggtgga gttcattcac accgccacgc tgctgcacga tgatgtggtg gatggttccg
    15601 cgctccgccg gggcagtccg acggtgaacg ccctgttcgg caacgctgcc agcgtattgg
    15661 tgggtgattt cgtctattcg cgcgctttcc agatgatggt ggaagtggac agcatgcggg
    15721 tgatgcgagt gctggcggac gccaccaacg tgattgccga gggggaagtg ctgcagttgc
    15781 tgaactgcca taacgccaac attgacgaac aacgttatct gcaagtgata cgctgcaaga
    15841 ccgccaagct gttcgaggca gccacccgtc ttggcgcgat actgggccat gccgacagcg
    15901 ccgaagagga ggcgatggcg agttatggca tgcacttggg catcgcgttt cagctcatcg
    15961 atgatgtgct tgattattcc ggcgacctaa gccagaccgg gaagaatatc ggcgacgatc
    16021 tggcggaagg caaacccacc ttgcccctca tttacgccat gaaacacggc aaccctgagc
    16081 aggcggctct catccgcaag gccgttgagc agggcggccg cgaggaattt gctacggtgc
    16141 tcgacacgat tcacaagact cgcgccctgg agtatgcgcg gacgcaagcc aagaccgaag
    16201 cgcaagccgc atgtgccgcc tgcgctatgc tgccgcgttc aaattaccgc gattctttgc
    16261 tacaattggc ggactttgcg gtcagcagaa gttattagat ttaaaatcta gaacccatc
//
LOCUS       DATIOW010000203         6146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_2016913, whole genome shotgun sequence.
ACCESSION   DATIOW010000203 DATIOW010000000
VERSION     DATIOW010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2016913"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..99)
                     /locus_tag="VLL03_06775"
     CDS             complement(<1..99)
                     /locus_tag="VLL03_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004534004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSS47098.1"
                     /translation="MTTSLVTGASGFVGSALARKLVAAGHRVKVLLR"
     gene            complement(116..820)
                     /locus_tag="VLL03_06780"
     CDS             complement(116..820)
                     /locus_tag="VLL03_06780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS47099.1"
                     /translation="MIGIVAALPLEVRCLLGRKVKPGERISLNDAGWIQVSGIGREKA
                     LRAAHSLLDAGATALLSWGVTGGLDPKLVPGTLVLPRQIHAADQNRFPVDEAWRSRIH
                     TCVARHVMVSDGALIEGLSVIAGPAQKRALAQASGAVAVDMESAAVGQAAKQAGVPFV
                     AVRAVVDTADSTLPRSVLRANDEYGRLRIAKLLFSLIAHPADFGALIGLGTNFRAAQA
                     TLQTIARLAGPKLCLQ"
     gene            complement(817..2781)
                     /gene="shc"
                     /locus_tag="VLL03_06785"
     CDS             complement(817..2781)
                     /gene="shc"
                     /locus_tag="VLL03_06785"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HSS47100.1"
                     /translation="MPERYQELAASGNPARAREHSNVRLIGTRQAALWETISAARDAL
                     LALQHEKGYWCFELEADCTIPAEYILMMHFMDEIDVELERKIAVFLRRNQDVHGGWVL
                     YHGGDFDISCSVKIYYALKIAGDSPDAPHMVKAREAILAHGGAARCNVFTRIALALFG
                     QIPWRGVPIVPVEIMLLPRWFPFHLSKVSYWSRTVMVPLAILCSLKAQARNPRGTGIR
                     ELFTVAPEQEKNYFPVRSRLNWLLLRLERFSRFLEPAIPGFIRRHAMKKAEQWFIERL
                     NGEHGLGAIFPAMVNAHEALARLGYPADHPYRQTTSRALKKLLVINEDSAHCQPCFSP
                     VWDTALAVLALHQAEGEQATPEVLRALDWLKERQLLDEPGDWRDSRPELRGGGWPFQF
                     GNGHYPDIDDTAAVGWAMHRVDRVRYAESLQRAAEWVCGMQSKNGGFAAFDVDNTYYY
                     LDEIPFADHGALLDPPTSDVSGRCIALLAPLGGYQAVWRRCLEFLKSEQEKNGSWFGR
                     WGTNYIYGTWSVLAALELAREAPGQLYVRRAVEWLKRMQRPDGGWGEGNDTYFYSEKA
                     GQGNSSTAFQTAWAMLALMAAGEADAPEVARGAEYLLRTQQANGLWNDPGFTAPGFPR
                     VFFLKYHGYDKFFPLWALARYYNQRSHHGA"
     gene            complement(2801..3865)
                     /locus_tag="VLL03_06790"
     CDS             complement(2801..3865)
                     /locus_tag="VLL03_06790"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HSS47101.1"
                     /translation="MPDWQAEPGKQLSGEAYQDHILQGVSRTFALTIPQLPSALRSAV
                     SNAYLLCRIADTIEDEPALSAGQKRYFSDQFIKVVAGEAAPETFAAEFYPLLSSSTQE
                     AEKDLIKNTPQVIRLTHGFSRNQRAAMERCVRIMANGMALFQSNPSPYGLKDLPQLDS
                     YCYHVAGVVGEMLTELFCNYSVEIGKNRQKMLELATSFGQGLQMTNILKDIWEDLGRG
                     ACWLPQDIFRESGFDLRNLARERHDAAFAAGLEKLIAIAHAHLRNALAYTLLVPRYET
                     GIRKFCFWALGMAVLTLRKISANRAFTSGNEVKISRRAVKATILATQLTIGSDTMPKW
                     LFNVTAFGLPRAASLQAAPG"
     gene            3981..5141
                     /gene="hpnI"
                     /locus_tag="VLL03_06795"
     CDS             3981..5141
                     /gene="hpnI"
                     /locus_tag="VLL03_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HSS47102.1"
                     /translation="MRLEPVLRKLTIIAPTLIVFTLTLASLAYLAFALLRVIAFGKQR
                     AGPSEYRPPISLLKPVCGLEPELYQNLRTFCDQDYPDYQIIFGVRDPNDSAITVIERL
                     IQEFPLRHAALIVDETVTGTNFKVSNLANMYRAVEHDLLVVADSDMRVGRDYLGAVVA
                     PFADPQVGAATCLYVGVPVDGFASRLGAMFINDCFAPSVLVALSFQELRYCFGATMAV
                     RRPALEKMGGFAVLADQLADDYMLGKRIVEQGLKVALPAYVVENHVWESSFIALFLHE
                     LRWARTIRAMQPVGYAFSFITYPVAVSALFVLIFPMKAAGLTLLAVSVIMRVLLHWAV
                     HASFRIPGPTQAWLAPFRDLLSFTVWAVSFFGRSVQWREQNFSVQPDGQLTK"
     gene            5161..>6146
                     /locus_tag="VLL03_06800"
     CDS             5161..>6146
                     /locus_tag="VLL03_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSS47103.1"
                     /translation="MKTLFLNPPSYEGFDGGAGSRYQARREIRSFWYPTWLAQPAALI
                     PGGKLIDAPARGLSLNDILPLANDYELAILHTSTPSFESDAKVAEALKAKNPKLMIGF
                     IGAHVAVQSELSLKHSPAIDFVARNEFDFTVHDVASGRPLAEIDGISHKVNGEVVHNR
                     DRAMIENMDQLPWVVDVYKRDLVIEDYFIGYLQHPYVSLYTGRGCKSRCTFCLWPQTI
                     GGHRYRTRSPENVVEEIARAKEYFPQVQEFFFDDDTFTDDLPRAEEIAKGLGKLGVTW
                     SCNAKANVPYASLKVMKENGLRLLLVGFETGSQQILLNIKKGMHVDMARHFMA"
BASE COUNT         1232 a         1894 c         1743 g         1277 t
ORIGIN      
        1 gcgcagcaat actttaacgc ggtgtcccgc ggcgaccagt ttgcgcgcaa gcgccgagcc
       61 gacaaaaccc gaagcgccgg taacaaggct ggtagtcatg cgaccgcggc aattcctact
      121 gcagacataa tttcggaccg gcgagccggg cgatggtctg cagcgtcgcc tgggccgcgc
      181 ggaagtttgt gcccaacccg atgagcgcgc cgaagtccgc agggtgggca atcagggaaa
      241 acaaaagttt ggcgattcgc aaacgcccgt actcgtcgtt ggcgcgcagt acgctgcggg
      301 gcagggtcga gtccgcggtg tccaccactg cgcgcactgc gacaaatggc acgccggctt
      361 gctttgccgc ttgaccaacc gccgcgcttt ccatatccac cgccaccgca ccgctcgctt
      421 gcgccaaggc ccgcttttgg gcgggaccgg caatgacgga aagaccttcg atcaacgcgc
      481 cgtcgctcac catgacgtgt cgggcgacgc aagtgtgaat acggctgcgc caggcttcgt
      541 cgactggaaa ccggttttgg tctgcagcat ggatttgccg gggcaatacc agggttcccg
      601 gaaccaactt gggatcaagc ccgccggtga caccccagct caacaacgca gtggctccgg
      661 catcgagcag ggagtgcgcc gcacgcaggg ctttctccct gccgatgccg ctgacttgaa
      721 tccagccggc gtcattaagt gaaatgcgtt cgcccggttt gactttcctc cccagcagac
      781 agcgcacctc aagcggcagc gcggcgacga tgccgatcac gctccgtggt ggctgcgctg
      841 attgtagtaa cgcgccagtg cccacaacgg gaaaaacttg tcgtaaccgt ggtactttag
      901 aaaaaacacg cgagggaagc ctggcgccgt gaatcccgga tcattccaca agccattagc
      961 ctgctgcgtg cgcagcaggt attccgcacc gcgcgcgact tccggcgcat ccgcttcgcc
     1021 cgccgccatc agcgcgagca tcgcccaagc cgtctggaat gcggtgctgg aattgccctg
     1081 gccggctttt tccgaataga aataagtgtc gttgccttcg ccccagccgc cgtcgggacg
     1141 ctgcatgcgt ttaagccact ccaccgcgcg gcggacatac aactggcccg gcgcttctcg
     1201 ggcaagctcc agcgccgcca gcaccgacca cgtgccgtaa atgtaattcg tgccccaacg
     1261 accgaaccac gatccattct tttcctgctc gcttttcagg aattcaaggc agcgtctcca
     1321 cactgcctga tacccgccga gcggtgccag cagcgcaatg cagcgcccgc tcacgtcgct
     1381 ggtcggtgga tcgagcagtg cgccatgatc ggcaaacggg atttcgtcga gatagtaata
     1441 agtattgtcg acatcgaatg cggcaaagcc gccgttcttc gattgcatgc cgcacaccca
     1501 ttccgccgcg cgctgcaagc tctcggcgta gcgcactctg tccacccggt gcatcgccca
     1561 gcccaccgcg gcggtgtcat cgatgtccgg ataatgacca ttgccaaact gaaatggcca
     1621 gccgccgccc cgaagctcag gcctggaatc gcgccagtca ccaggctcat cgagaagctg
     1681 ccgttccttg agccagtcga gcgcgcgcaa aacttctggc gtggcttgtt cgccctcggc
     1741 ttgatgcagc gccagtaccg caagtgcggt atcccaaacc ggagagaaac acggctggca
     1801 atgcgcagag tcttcattga tcaccagcaa tttcttgagc gccctgctgg tggtctgccg
     1861 gtaaggatga tcggcgggat aacccagccg agccagcgct tcatgcgcat tgaccattgc
     1921 ggggaaaatc gcgcccagtc cgtgttcgcc gttaagccgt tcgataaacc actgctcggc
     1981 cttcttcatt gcatggcggc ggatgaagcc gggaatcgcc ggctccagga acctgctgaa
     2041 gcgctccaga cgcagcaaca gccagttcag gcgcgagcgc accggaaaat aatttttttc
     2101 ctgctccggc gcgacggtga aaagctcgcg aatacctgtc ccgcgcggat tcctggcctg
     2161 ggctttcagg ctgcagagaa tcgccagcgg caccatcacg gtgcgcgacc agtaggacac
     2221 cttgctcaaa tggaacggga accagcgcgg cagcagcatg atttccaccg gcactatggg
     2281 cacgccgcgc cagggaattt gcccgaacag ggcgagggcg atgcgggtaa atacattgca
     2341 gcgcgccgcg ccgccatgcg cgaggatcgc ctcgcgcgcc ttcaccatgt gcggcgcgtc
     2401 cgggctgtcg cccgcgatct tcagcgcgta atagatcttg accgagcagc tgatatcgaa
     2461 atcaccgccg tgataaagca cccagccgcc gtgcacgtcc tggttgcggc gcaggaacac
     2521 cgcaattttt ctttcgagct cgacgtcgat ttcatccatg aaatgcatca tcaggatgta
     2581 ttcggcggga atggtgcagt cggcttcgag ttcgaagcac cagtagcctt tttcgtgctg
     2641 cagtgcgagc agcgcatcgc gcgcggcgct gatcgtttcc cataatgccg cttggcgcgt
     2701 gccgatcaag cgcacattgc tgtgttcgcg cgctcgggcg gggttgcccg atgctgccag
     2761 ctcttggtat cgttccggca ttcgttattt ctccgaatta ctatcctggc gcagcttgca
     2821 acgaggcggc acgcggcaac ccaaacgcgg tcacgttaaa aagccacttc ggcatggtgt
     2881 cgctgccgat cgtaagctga gttgccaaaa tcgtcgcttt caccgcgcgc cgggaaatct
     2941 tcacttcgtt gcccgacgta aaagcgcggt tggcgctgat tttgcgcaaa gtcagcaccg
     3001 ccatgccgag cgcccagaag cagaacttgc ggatgccggt ctcatatctc gggacgagca
     3061 gcgtgtaagc caaagcgtta cgcaaatgcg catgggcaat ggcaatcagt ttttccagcc
     3121 cggccgcaaa ggcggcatcg tggcgctccc tggcaagatt gcgcaagtca aaaccgcttt
     3181 cacgaaatat atcctgcggc agccagcagg cgccgcggcc gaggtcttcc cagatgtctt
     3241 tcaaaatatt ggtcatctgc aaaccctggc caaacgaagt cgccaattct agcatttttt
     3301 gacggttttt cccgatttca acggagtaat tacaaaacag ttcggtgagc atttcgccca
     3361 ccacaccggc cacgtggtag cagtagctgt cgagctgcgg caggtctttc aagccataag
     3421 gacttgggtt ggactggaac agcgccatgc cgttcgccat gatgcgcacg cagcgctcca
     3481 tcgccgcacg ctggttgcgg ctgaaaccgt gggtgaggcg gatgacctgc ggcgtatttt
     3541 tgatcagatc cttttccgct tcctgcgtgg aactcgagag cagcggataa aactcggcgg
     3601 cgaaagtttc aggcgccgcc tcaccggcaa ccaccttgat gaactggtcg gagaaatagc
     3661 gcttttgccc ggcgctcaag gccggttcgt cttcaatggt gtcggcgatg cggcacaaga
     3721 gataggcatt gctcaccgcg ctgcgcaaag cagatggaag ctgtgggata gtcagcgcaa
     3781 acgtgcgcga taccccctgc aggatatggt cctgatacgc ttcaccgctg agttgtttgc
     3841 ctggctctgc ctgccaatcc ggcatcttga aaatccccgg ttgccacgcc tgttagcgtc
     3901 cggcgcaagg ggcacattgt agctcaaatc gcctggaagg gctgctgcac cccattatct
     3961 gctgatgggc atgttaaaat ttgcgcttag aaccagtcct aaggaaactc accatcatcg
     4021 cccctaccct catcgttttc acgctcacgc tggcttcact ggcctacctt gcttttgccc
     4081 tgctgcgcgt catcgctttc gggaaacaac gcgcaggccc gagtgaatac cggccaccca
     4141 tcagtttgct gaagccggtt tgcgggttgg aacccgagct ctaccagaac ctgcgtacgt
     4201 tttgcgacca ggattatccc gattaccaaa tcatcttcgg cgtgcgcgat ccaaatgact
     4261 cggcgattac cgtcatcgaa cgcctcattc aggaatttcc gctacgccat gctgcgctga
     4321 tcgtggacga aaccgtcacc ggcaccaatt tcaaggtgag caacctcgcc aatatgtacc
     4381 gcgcggtaga gcatgatctg ttggtcgtcg ccgacagcga tatgcgagtg ggacgtgatt
     4441 atctcggcgc ggtcgtcgcc ccgttcgccg atccgcaagt tggcgccgcg acttgtcttt
     4501 acgtgggcgt gccagtcgat ggcttcgctt cgcggttggg cgcgatgttc atcaacgatt
     4561 gtttcgctcc ctcggttttg gtcgcgcttt ccttccagga gttgcgctac tgcttcggtg
     4621 cgaccatggc cgtacggcgc ccggcactgg aaaaaatggg cggctttgcc gtcctcgccg
     4681 accaacttgc cgatgattac atgctcggca agcgcatagt ggaacaaggc cttaaagtcg
     4741 cattgccggc ttacgtcgtt gaaaaccatg tatgggaatc cagcttcata gcgctgtttc
     4801 tgcacgagct gcgctgggcg cgcaccatcc gtgccatgca acctgtcggt tatgcgttct
     4861 ccttcatcac ttacccggtt gcggtttcgg ccctcttcgt cctgattttt cccatgaaag
     4921 ccgcagggtt aacgttactg gcagtttcgg ttataatgcg cgttttactg cactgggcgg
     4981 tacacgccag tttccgcata ccgggaccaa cccaggcttg gctagcgcca tttcgggatc
     5041 ttttgagttt cacggtttgg gcggtaagct tctttgggcg aagcgtgcaa tggcgggaac
     5101 agaatttttc ggtccagccg gacggacagt tgacgaaata aaggagcacg caatctcatg
     5161 atgaagaccc tgtttttgaa ccctccgtcg tatgaaggat tcgacggcgg cgccggctca
     5221 cgctaccagg cgcggcggga aatccgctcg ttctggtatc ccacctggct cgcccagccc
     5281 gcggcactca tccccggcgg caaactgatc gacgcgcccg cgcgcggcct gtcgcttaac
     5341 gacattctgc cgctcgccaa cgattatgaa ctcgccattc tgcataccag cacgccctct
     5401 tttgaatccg acgccaaagt tgctgaagcg cttaaagcca aaaaccccaa gctcatgatt
     5461 ggtttcatcg gcgcacacgt ggcagtgcaa tccgagcttt cgcttaagca ttcgcccgcg
     5521 attgacttcg tcgcgcgcaa cgaattcgat ttcactgtcc acgatgtcgc ctccggtcgt
     5581 ccgcttgcgg aaatcgacgg catcagccac aaggtcaatg gcgaggtggt gcataaccgt
     5641 gaccgcgcca tgatcgaaaa catggaccag ctgccgtggg tcgtcgacgt ctataagcgc
     5701 gacctggtga tcgaagacta tttcatcggc tatctgcagc atccctacgt ttcgctttac
     5761 accggtcgcg gctgcaaatc gcgctgcacc ttctgcttgt ggccgcagac catcggcggc
     5821 caccgttacc gcacccgcag tcccgaaaat gtggtggagg aaatagctcg cgccaaggaa
     5881 tatttcccgc aggtccagga gttcttcttt gacgacgaca ctttcaccga cgatctgccg
     5941 cgtgccgagg agattgccaa ggggctcggc aagctggggg tcacttggtc gtgcaacgcc
     6001 aaggccaatg tgccgtacgc tagcctgaag gtaatgaagg aaaacggctt gcgcctgctg
     6061 ttggtcggct tcgagaccgg cagccagcag atcctgctca acatcaagaa gggcatgcat
     6121 gtagacatgg cgcgccactt tatggc
//
LOCUS       DATIOW010000204        12609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_902598, whole genome shotgun sequence.
ACCESSION   DATIOW010000204 DATIOW010000000
VERSION     DATIOW010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12609
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902598"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            464..1105
                     /locus_tag="VLL03_06805"
     CDS             464..1105
                     /locus_tag="VLL03_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HSS47104.1"
                     /translation="MVFYYGSGSPYAWRVWLALEYKQLAHEMKAISFSAGDTRKPAFL
                     KLNPRHRVPVLVDDGFALYESSAIVEYIEDKYPKSGNGALFPQAPAERATARRLILEV
                     DNYLRTAVDPMLRQIFFKPEAEWDAQVIAGSREEFLAELRNFEGQVRGEYLLGKLSAA
                     DFALYPMLALALRLEKKKNDLGLRAAVGPKLSAWMKRIEALPYFRKTWPPHWK"
     gene            complement(1115..2302)
                     /locus_tag="VLL03_06810"
     CDS             complement(1115..2302)
                     /locus_tag="VLL03_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HSS47105.1"
                     /translation="MKQERLRENARIALRMSDIQPFHVMELLARARALEAQGRSIVHM
                     EIGEPDFPSPQPVIAAGMEALKRGNIHYSPALGLPQLREAIAGFYMQRHGLRVSPSRI
                     VVTPGASGALLLTLGILINPGDKVLMSDPGYPCNRHFVRLLEGVPTGIGTDATTGYQL
                     THELIAQYWDRATTAVMIASPANPTGTLVSREQLHAIVKKTHALRGRLIVDEIYHGLV
                     YESETTTALSLSDEVFVINSFSKYFGMTGWRLGWLVAPEAYVREADKLAQNVFLAAST
                     PAQYAALAAFAPETLRILEERRAEFRRRRDYLLPALRNLGFVIPATAQGAFYLYADCS
                     RFSTDSETFAHELLEHAGVAVTPGIDFGKHQASRHVRFAYTTSLENLREGMRRLEVFL
                     RKS"
     gene            complement(2299..2763)
                     /locus_tag="VLL03_06815"
     CDS             complement(2299..2763)
                     /locus_tag="VLL03_06815"
                     /inference="COORDINATES: protein motif:HMM:NF040448.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6505 family protein"
                     /protein_id="HSS47106.1"
                     /translation="MKFLKAVRLDASDSRVYAQEGAAEDGEWLVSGGYAVCDLASGTH
                     LRPNCHCDTSFVGVKTHGRCTLAEVVEIDEATYREHIETLIRHFLEDLNAPSEEAARK
                     VAEEEVAYTVDLCESFNTEVWITVKRTPKTDSEGFDEHYQVFKRLMIGEHKL"
     gene            2874..3302
                     /locus_tag="VLL03_06820"
     CDS             2874..3302
                     /locus_tag="VLL03_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HSS47107.1"
                     /translation="MKETLQPGLTFEFKFKIPETKTVPHLYPESQMFREMPSVLATGF
                     MVGLMEWACIEALRPHLDWPREQTLGTHVNFSHTAPTPPGFTVTVSVKLKKIEGRKLT
                     FSVSAHDGVDAISAGTHERFVIDAAKFKENIYEKSRYYNK"
     gene            3409..3861
                     /locus_tag="VLL03_06825"
     CDS             3409..3861
                     /locus_tag="VLL03_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS47108.1"
                     /translation="MPDTIRKIEYFTANVSNKSGEGVRALKALRDANVNLLAFSGFPR
                     GGRAQIDFIPEDSAALKAAAKKAKLKLSSKKTGFLIQGSDRVGALLDTLGRLAAAKIN
                     VTALDAVTAGEGRYGAIFWVKPKDVAKTAKLLGVSAEKQAGSAAYPVL"
     gene            complement(3838..4734)
                     /locus_tag="VLL03_06830"
     CDS             complement(3838..4734)
                     /locus_tag="VLL03_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HSS47109.1"
                     /translation="MRFLTFFRDGEARVGVLRGERVVDVATQAKRLGEQLPVDIFDLV
                     SLIASGKVGLNAVRKLLQAPAEDLPLAELKLLAPIPRPRKNVFCVGWNYLEHFAEGEA
                     IRQSGQELPAHPVFFSKTPTAVTGPYETIPYDAQVSAEIDWEVELGVIVGKNGKNITE
                     SQAHEYTFGYTVVNDISARDLQRRHGGQWLKGKSLDGACPMGPWIVTADEINPDNLRV
                     LTRVNGVTKQDSNTRYLYFKIPRLISELSLGMTLEAGDIISTGTPPGVGFARKPPEFL
                     KRGDLLETEIEGIGRLQNRIGG"
     gene            complement(4766..4903)
                     /locus_tag="VLL03_06835"
                     /pseudo
     CDS             complement(4766..4903)
                     /locus_tag="VLL03_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            5193..5900
                     /locus_tag="VLL03_06840"
     CDS             5193..5900
                     /locus_tag="VLL03_06840"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSS47110.1"
                     /translation="MMDRKIAVVTGANRGIGFEICRQLARKEITVVLTSRDEAKGQMA
                     VAQLRSEGLEAEFHQLEVTDGPGIRRLAHYLERKYGAADILVNNAGIMIDPKSSNLLD
                     AELKVFRQTLEINVYGPLLLCQALIPLMRRKNYGRIVNLSSGLGQLSEMGAGTPAYRV
                     SKVALNAVTRMLAAELKGTNILVNSMCPGWVRTEMGGLKAPRAVEQGADTAIWLATLP
                     HGSASGGFYRDRKAIPW"
     gene            5924..6514
                     /locus_tag="VLL03_06845"
     CDS             5924..6514
                     /locus_tag="VLL03_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HSS47111.1"
                     /translation="MLDRDWLASRLAEALDLPLEQGRPEQAFIEPEQAIAAAVLVPLV
                     RRDAGLNVLFTRRTTHLADHAGQISFPGGRVESADVSRKETALREAEEEIGLPRDKVD
                     VLGVLPDYFVVTGFRITPVVGWIEAPFPVRLDRFEVADVFEAPLEFFLDPANHQIHST
                     IYKGEPRRYYSMPYAGHYIWGATAGILLNLCQALRA"
     gene            6721..6984
                     /locus_tag="VLL03_06850"
     CDS             6721..6984
                     /locus_tag="VLL03_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS47112.1"
                     /translation="MPIKRKDQEISMLRQEIEMLMGERQNLLRVTGAAAVFVANMDSK
                     SLPEETYEAAEQLAEGLNALPEDTLRDALELVKTRAGPAAVKK"
     gene            7004..8164
                     /locus_tag="VLL03_06855"
     CDS             7004..8164
                     /locus_tag="VLL03_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HSS47113.1"
                     /translation="MDAQVAKPKVGLILTGGGARAAYQVGVLRAISNLLPKNAANPFP
                     IICGSSAGAINAAALAVNAGNFRTGIGRLMAVWRNFHVHQVYRSDPVGVMNSGVRWLA
                     ALMVGGLGKHNPTSLLDNSPLAELLRHKFEFKGIQDSIDCGALYALSITASGYASGQS
                     VTFYQAVPTMSPWQRARRLGIPTQIGLEHLLASSAIPFMFSAVKINREYFGDGSMRQI
                     APISPALHLGAERVLVVGVTQAEQPNRARGEDYPSLAEIAGHALNSIFLDGLEVDLER
                     LQRINKTINLIPSHALEKSGLSLRHVDVLVISPSESIENIAQKHVRALPRSIRFLLHG
                     VGVTRRRGANLLSYLLFEKPFCRDLIDLGYKDAIGRKGEILEFLKEVTTAES"
     gene            complement(8168..8605)
                     /locus_tag="VLL03_06860"
     CDS             complement(8168..8605)
                     /locus_tag="VLL03_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS47114.1"
                     /translation="MGEKPQTISQQLHSNLVAIISLVVALSGLFYNNWREKVIEENRS
                     IRIASFEVLKNLGELQLVVDYAHYQKNKQLGNPITGWGRVLLIKDLAQVIPDPVTKTT
                     EELYRVWGAEFEKIESDEKSVERVTQAINTTRQSVLQALSQLK"
     gene            8811..9317
                     /locus_tag="VLL03_06865"
     CDS             8811..9317
                     /locus_tag="VLL03_06865"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HSS47115.1"
                     /translation="MSRAFVKEQDDKPAIDELPERPQSPHPNYVTPKGHALLQARCQE
                     LLQQRATLAADSEDSLAAQQLMQVERDLRYFQVRLERAIVVDPAAQPADEVHFGAVVS
                     LLDQNGKKHEFAIIGEDEADAAKGRISWVSPLAHAIIGLRVGDNVVWKRPVGDLELEI
                     TAICYPRG"
     gene            9319..10245
                     /locus_tag="VLL03_06870"
     CDS             9319..10245
                     /locus_tag="VLL03_06870"
                     /inference="COORDINATES: protein motif:HMM:NF005792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HSS47116.1"
                     /translation="MSLIALIAALLIEQFRPLGPRNRFYAWFARYANFLERHFNAGAS
                     QHGVAAWLLALLPPLLAAGTAYYFLQRIHPVLSWLWNVGVLYLTMGFRQFSHTFTLIH
                     QALRAGDLEKARELLGQWRGSSATEFSINEIVRVVIEEGLVCSHRYVFGVIFWFALLP
                     GPLGAVLYRLAASLSQKWGSREDREFATFGRFAERAFEFIDWLPLRLTAVSFAIVGDF
                     EDAVYCWRAQALTWLKPQLGILLASGAGAIGVRLGEALHQYGDLHHRPELGLGDEADV
                     EHLESTIGLVWRALLLWLLVLLLLNFASWVGG"
     gene            complement(10487..10789)
                     /locus_tag="VLL03_06875"
     CDS             complement(10487..10789)
                     /locus_tag="VLL03_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS47117.1"
                     /translation="MKYGLILLALLAVFPAAADLFPNADPKIGKSLHEKSCSACHAAM
                     FGGDAKLYTRSNRIVKTPQQLLARVSICNANTSAGLFPEEEEHIAAYLNQQYYKFK"
     gene            complement(10801..12051)
                     /locus_tag="VLL03_06880"
     CDS             complement(10801..12051)
                     /locus_tag="VLL03_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HSS47118.1"
                     /translation="MHTFTVVFLAALLATTTTQLWLASRHVRHIQANRERVPAEFATQ
                     INLPAHQKAADYSSAKARLHMAHAVLDAVLLLVLTLGGGLQVLSGLWASLFGSGLAFG
                     MALIVSVAIIVGVVETPLNIYRTFVIEARFGFNQMTPALFVMDLLKQMLLAATFGIPL
                     LFCVLWLMAKMGEYWWLYVWLTWVAFNILVLAIYPNYIAPLFNKFSPMEDPELKRRVE
                     RLLEKCGFKSQGLFVMDGSKRSNHGNAYFTGFGAAKRIVFFDTLLSRLEPPEIEAVLA
                     HELGHYRRHHVIKRMLWTFVVSLAFLWLLGVLMDKTWFYAGLNVATPSTAMALLLFFM
                     ITPIFTFLLQPLLARYSRLHEFEADQYAVQYSSAPDLVRALVKLYKDNASTLTPDPLH
                     SAFYDSHPPAATRIARLQAASSSG"
     gene            12203..>12609
                     /gene="orn"
                     /locus_tag="VLL03_06885"
     CDS             12203..>12609
                     /gene="orn"
                     /locus_tag="VLL03_06885"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HSS47119.1"
                     /translation="MPQDDNHLIWIDLEMSGLNPDIDRVLEVALVVTDSNLNTVAEAP
                     VLVVRQPDAVLDSMDDWNKSTHAKSGLIDKVKASNLSEADVEAQMIAFLSQYVPAKGS
                     PMCGNSICQDRRFLARYMPQLEAYFHYRNLDVST"
BASE COUNT         2646 a         3504 c         3734 g         2725 t
ORIGIN      
        1 tccgtgcaag tcttgatcca gtgtttcatc tcgggtataa accggcccgg ccgctgcagc
       61 acctgcccag accagcaaca cgaacaataa aaatcgtttc atggctatgt tatagactcg
      121 gtttgtcctc cagggttgcg cgtccgaagc gctggtatgg acgcttctgg cgttgcccag
      181 gctcggggtg tatcgtaatg ggctgcaagt attatcccaa atcagctccc gcaaaactca
      241 tcattcaggc taggccatga aattatttaa gttttcctag gtcttattgg ctttttgctt
      301 tgcgcatttg caccaagtgc gtcggtgcag aatgccacgg aaattaccga agccgatgaa
      361 gtcgaaatcc gcaaggtcat cagccaaacg tggtttccca cttgaatcgt taatctgccc
      421 acgttaagcg gcggcaatca ctgcaggaga taatatcgtg accatggttt tttactacgg
      481 ctcgggctcg ccttatgctt ggagggtctg gctcgccctc gaatacaagc agcttgctca
      541 tgagatgaaa gccatatcat tttcggcggg cgatacgcgc aagccggcgt tccttaagct
      601 caatccccgc catagggtac cggtgctggt ggatgacggc ttcgcactct acgaatcttc
      661 cgcgattgtg gagtatatcg aagataaata ccccaagtcg ggcaatggcg cgctgtttcc
      721 gcaagcaccg gccgagcgcg ctacggcacg gcgcctgatt ctggaagtgg acaattatct
      781 gcgcacggcc gtggatccca tgctgcggca gatttttttc aagccggaag cggagtggga
      841 cgcacaggtt attgccggga gccgggaaga atttttagcc gagctccgga atttcgaggg
      901 ccaagtgcgc ggcgaatatc tgctgggcaa gctcagcgcg gcggatttcg cactttatcc
      961 gatgctggcg ctggcactgc ggctggaaaa gaaaaaaaat gatttgggcc tgcgtgctgc
     1021 cgtcggtccc aagctttccg cgtggatgaa gcgcatcgag gcgctgccct atttccggaa
     1081 aacctggccg ccgcactgga aatgaaactg cgcatcagct tttgcgcaga aaaacctcaa
     1141 gacggcgcat gccctctcgc agattttcca gcgaagtagt gtaggcaaag cgcacatggc
     1201 gcgacgcttg gtgcttgccg aaatcgattc ccggcgtaac cgccacgccc gcgtgttcca
     1261 acagctcgtg cgcgaaggtt tcgctgtctg tgctgaagcg gctgcagtcc gcgtagagat
     1321 aaaaagcgcc ttgcgctgta gctggaatca cgaaacccag attgcgcaaa gcgggaagca
     1381 aataatcgcg gcggcgccgg aattcggcgc ggcgctcctc aagaatacgc agggtctcgg
     1441 gtgcaaacgc agccagtgcc gcatattgcg ccggcgtgga cgcggcgaga aaaacgttct
     1501 gtgccagctt gtccgcttca cgcacatagg cttccggcgc caccagccag cccaggcgcc
     1561 acccggtcat gccgaaatat ttggagaagc tgttgatgac aaacacttca tcggatagcg
     1621 acaaggcggt agttgtttca ctctcataaa ccaatccgtg ataaatctca tccacaatca
     1681 accggccgcg gagcgcgtgt gtttttttta cgatggcatg caattgttcg cggcttacca
     1741 gcgtgccggt gggattagcg ggcgaggcaa tcatgactgc ggttgtcgca cggtcccaat
     1801 attgggcaat cagttcatgg gtgagctgat aaccggttgt ggcatcagtg cctatgccag
     1861 tgggaactcc ttccagcagg cgcacgaaat gccggttgca ggggtaaccc ggatcgctca
     1921 tcaacacctt gtcgccggga ttgatcagga tgccgagtgt aagcaacagc gccccggatg
     1981 cgccaggcgt gacgacgata cgcgagggtg aaacccgcag gccatggcgt tgcatataaa
     2041 aaccggcaat ggcttcgcgc aattgcggca gtcccagcgc cggagagtaa tgaatgttgc
     2101 cgcgcttcaa cgcttccatt cccgcggcga tgaccggctg cggggaggga aaatccggct
     2161 cgccgatttc catgtggacg atggaacggc cctgagcttc cagtgcgcgc gcccgcgcca
     2221 gcaattccat cacatgaaag ggctggatgt ccgacatccg gagcgcgatg cgcgcgtttt
     2281 cccgcagcct ctcctgcttc acagcttgtg ttctcctatc atcagccgct tgaatacctg
     2341 gtagtgttca tcaaatcctt cgctgtcggt cttgggcgtg cgctttaccg taatccatac
     2401 ctcggtattg aagctttcac acagatccac ggtgtaggcc acttcttctt ccgcgacttt
     2461 ccgcgccgcc tcttccgaag gcgcgttcaa gtcttcgaga aaatgccgga tcagggtttc
     2521 aatgtgttcc cggtaagttg cctcgtcgat ctccaccact tcggcgaggg tgcagcggcc
     2581 atgtgttttg acaccgacga aagaggtatc gcaatggcag ttggggcgca agtgcgtacc
     2641 gctcgccaga tcgcacacgg cgtagccgcc ggaaaccagc cattcgccgt cttccgccgc
     2701 gccctcctgc gcatagacac ggctgtcgga agcatcgagg cgcacggctt tcaggaattt
     2761 catcgccagt ctcggataaa ggaggaaaag cttaccgtaa acccggcggc ttcccaagcg
     2821 caaaaacggt tttttgcggt aacatcccgg cttaaatcca aataaggagc cccatgaaag
     2881 aaactttaca gcccggcctc acgtttgaat tcaaattcaa gatacctgaa accaagactg
     2941 tcccgcatct ttatccggaa tcgcagatgt tccgcgagat gccgtcagtg ctcgccaccg
     3001 gcttcatggt gggcttgatg gaatgggcct gcatcgaggc cctccgtccc catctggatt
     3061 ggccgcgcga gcagacgctg gggacgcatg tgaatttcag ccatactgcg cccacgccgc
     3121 cgggatttac ggtcacagtg agcgtcaagc tgaagaagat tgagggcagg aaactcacat
     3181 tttcggtgag tgcgcatgat ggcgtggacg ccatttccgc cggtacccac gaacgcttcg
     3241 tcatcgatgc ggccaagttt aaggaaaata tatatgaaaa atcaaggtat tataacaaat
     3301 gacgcccgct tgtgaaaagc gcgttcccag attgtcattt gcgtcaaact ttgtttaaga
     3361 ataaattatt agcagtggca attgtgccgc aagatggaag gaggtcacat gcctgatacc
     3421 attcgcaaaa tcgagtactt taccgctaac gtgtccaata agtcgggcga aggagtgcgg
     3481 gcgctcaagg cgctgcgcga tgccaacgtc aacctgctgg ctttttcggg ttttccgcgc
     3541 ggcggccggg cgcaaatcga tttcattccc gaggattccg cggcgctcaa agccgcggcg
     3601 aaaaaagcca agcttaaatt aagcagcaag aaaaccggtt tcctgattca aggcagcgac
     3661 cgggtgggcg cgcttttgga tacgctcggc cggctggcgg cagccaaaat caacgtcacg
     3721 gcgctggacg cggttaccgc gggtgaaggg cgctatggcg cgattttctg ggtgaagccg
     3781 aaggatgtgg ctaaaaccgc gaagctcctg ggtgtaagcg cagaaaagca ggcgggttca
     3841 gccgcctatc cggttttgta agcgcccgat tccctcaatt tcggtttcca gcaggtcgcc
     3901 ccgttttaaa aactcaggcg gcttgcgggc aaaccccact ccgggcggcg taccggtgga
     3961 aataatgtcg ccggcttcca gcgtcatgcc gagtgaaagc tccgaaatca gccgcgggat
     4021 tttgaaatac aaatagcggg tattcgagtc ctgcttggtc acgccgttga cgcgggtgag
     4081 gacgcgcaga ttgtcggggt tgatttcatc tgcagtcaca atccacggcc ccatcgggca
     4141 ggcgccgtcc aggcttttgc ccttgagcca ttgcccgccg tggcgccgct gcaagtcgcg
     4201 cgccgaaata tcattcacca ccgtgtagcc gaaggtgtat tcatgcgcct ggctttcagt
     4261 aatgtttttt ccatttttgc cgacgattac gcccagctct acctcccaat cgatttccgc
     4321 cgacacttgc gcatcgtacg gaattgtctc gtaagggccg gtcaccgcag tcggggtttt
     4381 gctgaaaaac accgggtgcg ccggtaattc ctgcccgctc tggcggatgg cttcgccttc
     4441 ggcgaaatgc tcgagataat tccagcccac gcaaaacacg ttcttgcgcg ggcgcggaat
     4501 cggcgcaagc agcttgagtt cggccagcgg caaatcttcc gctggcgctt gcagcaattt
     4561 gcgcactgca ttaaggccga ctttgcccga tgcgatcagc gaaaccaggt cgaaaatgtc
     4621 cacaggcagt tgctcgccca accgcttggc ctgagtggcg acatccacca cgcgctcgcc
     4681 gcgcaatacg ccgacccgcg cctcgccgtc gcggaaaaaa gtcaggaagc gcactgcgct
     4741 tcccgacggt gctgataccc cgcaatcact tcttgccgtt gatccatttg agatgcgaat
     4801 ccaccggatc gacgcttact gcaatcactt tcgcgttgcg tttttcgaac tcggccttga
     4861 ttttggccgt ggcgccgagc ttggaggcgc gcaccggaat aaaaccccgc gggtggggga
     4921 agggtaccgc tcatttgcca tcaatctgtt ggtgagaatg tgtcatgccc tcggaggatt
     4981 cctaagtggg atcgggagag agactgccta gtctaagtac cacgatgaat tcctcctaga
     5041 taacttgatc taaagtttcg aaggtttgca gctttttgca acagcaatat agcaggcggc
     5101 acgctgctat caaagtaacg ctttgcgcta tgattagaag tgatgattct atgtggcctt
     5161 gaacgcattc ccggcaaggg gcgcggagga gaatgatgga caggaagatt gcggtggtga
     5221 ccggcgccaa ccgcggcatc ggcttcgaaa tttgccgcca gctggcgcgg aaagaaatca
     5281 cggtggtgct taccagccgc gatgaggcaa agggccagat ggcggttgcg caactgcgca
     5341 gcgaggggct ggaggccgag ttccatcagc ttgaggtgac cgatggcccg ggcattcggc
     5401 gcctggctca ttatctcgaa cgcaaatatg gcgccgccga tatcctggtg aacaacgccg
     5461 gcatcatgat cgaccccaaa agctcaaatc tcttggacgc ggaattgaaa gtcttccggc
     5521 aaaccctgga aatcaatgtc tacgggccgc tgttgctctg ccaggcactg attccgctga
     5581 tgaggcggaa gaattatgga cgcatcgtca atctctcgag cgggctcggg caattgagcg
     5641 agatgggcgc cggcacgccg gcttaccgag tgtcgaaagt cgctttgaat gcggtcaccc
     5701 gcatgctggc cgccgagctt aaaggcacca atattctcgt caactcgatg tgcccgggct
     5761 gggtcagaac cgagatgggc ggcttgaaag cgccacgcgc ggtagaacaa ggggcggaca
     5821 cggcgatctg gctcgccacg cttccccacg gcagcgcgtc cggcggtttt tatcgcgaca
     5881 gaaaagccat tccctggtga ctacctcatc cgccagcaag ccgatgcttg atcgtgattg
     5941 gctggcctcg cggctggccg aggcgcttga tctgcctctg gaacagggaa ggccggagca
     6001 ggcttttatc gaacccgaac aggcgattgc ggccgcggtg ctggtgccgc tggtcaggcg
     6061 cgatgcagga ctcaacgtgc tatttacccg gcgcactacg catcttgccg accatgccgg
     6121 gcaaatcagt tttcccggcg gccgcgtgga atccgccgat gtgagcagaa aagagacggc
     6181 gctgcgcgag gcggaggagg aaattggcct gccgcgggac aaagtggatg tcttgggcgt
     6241 attgccggat tactttgtgg tcaccggttt ccgcatcacg cccgtggtgg gctggattga
     6301 agcgccgttt ccagtgcggc ttgaccgttt tgaagtggcg gacgttttcg aagcgccact
     6361 cgagtttttt ctcgatcccg ccaatcatca aattcacagc acgatttaca aaggcgagcc
     6421 taggcgatac tactccatgc cctatgcagg acactacatc tggggcgcca ccgccggaat
     6481 actgttgaat ctttgccagg cgctgcgtgc ttgacgctta ctggatgttc agaattttca
     6541 gcgctttggc cagcgtgtct atcgattctt ggtgcttgcc ggccttgtgc agcgcttcgt
     6601 cttcggcgcg cagctttttt acttccgcca attgttgcgt ggtggggtat tcgataaatc
     6661 tttcaacaac tggcgatgcg ctcgcaagct atggcaatat agaagccggg gcttacaaca
     6721 atgcctatta agcgcaaaga ccaggaaatt tcgatgctgc gccaggaaat cgagatgctg
     6781 atgggtgagc ggcagaatct gctccgggtg accggcgccg ccgcggtgtt cgtggccaac
     6841 atggacagca agagcctgcc ggaggaaacc tacgaggcgg cagagcagtt ggcggaagga
     6901 ttgaacgcgc ttcccgagga tacgctgcgc gacgcgctgg agttggtaaa gacgcgagcg
     6961 gggccggccg cagttaagaa gtagacgggc aacgggaggc aaagtggacg cccaggttgc
     7021 aaagcctaaa gtgggcctga ttctgactgg aggcggcgcg cgcgccgctt accaggtggg
     7081 cgtgctgagg gcgatttcca acctgctgcc aaaaaacgca gccaatccct ttcccatcat
     7141 ttgcggctct tccgccggcg ccatcaacgc ggcggcgctg gcggtcaatg ccggtaattt
     7201 ccgcaccggt atcggacgcc tgatggcggt ctggcgcaat tttcacgtgc accaagtcta
     7261 tcgttctgac ccggttggag taatgaattc cggcgtgcgc tggctggcgg cgctgatggt
     7321 cggcggcttg ggcaaacaca atccgacctc gctgctcgac aacagcccgc tcgcagaact
     7381 gctaagacac aaattcgaat tcaaaggcat ccaggatagc attgactgtg gcgcactgta
     7441 cgccttgagc atcaccgctt caggctatgc ttccggccag tcggtgacgt tttaccaggc
     7501 ggtgccgacc atgtcgccgt ggcagcgcgc gcggcgcttg ggcattccca cgcaaatcgg
     7561 cctcgagcat ctgctagcat cctcggcgat tcccttcatg ttttccgcgg tgaaaatcaa
     7621 ccgtgaatac ttcggcgacg gctcgatgcg gcagattgca cccatcagtc ccgccttgca
     7681 tctcggcgcc gaacgcgtac tggtggtggg ggtaacgcag gccgagcagc cgaaccgggc
     7741 caggggcgag gattatcctt cgctcgccga aatcgctggt catgcgctga acagtatttt
     7801 cctcgacggc ctggaagtgg atttggagcg gctgcagcgc atcaataaga ccatcaatct
     7861 gattccctcc catgcgctcg agaaaagcgg gctttctttg cgtcacgtag acgtgctggt
     7921 gatttcgcca agtgaatcga tcgaaaacat cgcgcaaaag catgtgcgtg cgttgccgcg
     7981 cagcatccgc ttcctgctgc atggagtcgg cgtgacccgc cgtaggggcg cgaacctgtt
     8041 gagctatctg ctgttcgaaa agccgttttg ccgcgatctg attgatctag gttataaaga
     8101 cgcgataggc cgcaaggggg aaatcctgga gtttctgaag gaggttacga ccgctgaaag
     8161 ctgaaagcta ttttagctgg cttaaggcct gcagcaccga ctgacgggtg gtgttgatgg
     8221 cttgggtcac ccgttccacg cttttctcgt cgctctctat tttttcaaat tccgctcccc
     8281 aaacccggta gagctcttcc gtggtcttgg tcacgggatc gggaatcacc tgggccagat
     8341 ccttgatgag cagcactctg ccccagcccg tgatagggtt gccgagctgc ttgtttttct
     8401 ggtaatgcgc gtaatccacc accagctgca gctcgcccag gttttttagc acctcgaatg
     8461 aagcgatgcg aatgctgcga ttttcttcaa tcactttttc gcgccagttg ttgtagaaaa
     8521 ggccgctgag cgccaccacc aggctgatga tggctactag attggagtgg agctgttgtg
     8581 aaatggtttg cggtttctcg cccattatgg cttcgtgggt tggtttgcgg tatattcaga
     8641 tacaggtttt attgtcctaa actatagtga aaacaggcag cgcgtcaaga cagtgcaaac
     8701 gcagatgaac aaaccgccgc taggcgcctc aaccgatgcc gccgcgaaga ccggcgcggc
     8761 cgcctgcgcc taatttttcc ctattttcaa cacttacgcg aagtcttgcc atgagccgag
     8821 cattcgtcaa ggaacaggat gacaaaccgg caatcgacga gttgccggaa cggccgcaaa
     8881 gcccgcatcc gaattacgtg acgcccaaag gccacgcctt gttgcaagcg cgctgtcagg
     8941 aattgttgca acagcgcgcg acgctcgctg cggacagcga ggactcactg gcggcgcaac
     9001 agcttatgca agtcgagcgc gatttgcgct atttccaggt gaggctcgag cgcgccatcg
     9061 tggtggaccc ggcagcgcaa cccgcggacg aggtgcattt cggcgccgtg gtgagtttgc
     9121 tggaccagaa tgggaaaaaa cacgagtttg ccatcatcgg cgaggacgag gcggacgccg
     9181 ccaagggccg gataagctgg gtgtcgccgc tggcccacgc gataataggc ttacgggtcg
     9241 gagataacgt ggtgtggaag cgtccagtgg gcgacctgga gttggaaatt accgccatct
     9301 gttacccgcg cggctgaaat gagcctgatt gctctgattg ccgcgctttt aatcgagcaa
     9361 ttccgtccgc tgggtccgcg caaccgcttt tacgcttggt ttgcacgcta tgccaatttt
     9421 ctggaacgtc atttcaatgc cggcgccagc caacatgggg tggctgcctg gctgctggcg
     9481 ctgctgccac ccctattggc ggcgggcacg gcctattatt ttctgcaacg gattcatccc
     9541 gttctaagct ggctgtggaa tgtcggagtg ctgtatctca ccatgggctt ccgccagttc
     9601 agccatacct ttaccctgat tcaccaggcg ttgcgcgccg gcgatttgga aaaagcgcgc
     9661 gagctgctcg gccaatggcg cggcagcagc gccaccgagt tttccatcaa cgaaatcgta
     9721 cgggtggtga tcgaggaggg gctcgtctgc tcgcatcgtt acgtgttcgg tgtgattttc
     9781 tggtttgcac tgctgcctgg ccccttgggc gcagtcttgt accgcctcgc cgccagcctc
     9841 agccagaaat gggggagccg tgaagaccgg gaattcgcca cgttcggccg ctttgccgag
     9901 cgcgcttttg agttcattga ctggctgcca ctgcgactca ccgcggtgag ctttgccatc
     9961 gtcggcgatt ttgaagacgc ggtgtactgc tggcgcgcgc aggcacttac ctggctcaag
    10021 ccgcagctcg gcatccttct tgccagcggc gccggcgcca tcggcgtgcg gctcggtgaa
    10081 gcgctgcacc agtatggcga tctgcaccat cgtccggagc tgggcctcgg cgatgaagct
    10141 gacgtggagc acttggagag cactatcggc ctggtgtggc gcgcgctgct gctatggctg
    10201 ctggtgctat tgctgctcaa tttcgcaagc tgggtcggcg gctaaggaac ttttgccaca
    10261 accgatttcc agtctttcct caggcggtga aaccatttca ttcgccgttc gctgattttt
    10321 ccgtttttcc ccgtcgagcg gttgccacag gcgtattggc gcttcttgtc gcggcttact
    10381 gatgatttcg ttcttatcga gctcgaccag gtattgccgg ccgggaccac gcgatgattt
    10441 gtccgtggag aagggaatga ctggtgttcc gcgggaaacg ggagagctac ttgaacttgt
    10501 aatattgctg attaagatac gccgcgatgt gctcttcctc ttccggaaac agcccggcgc
    10561 tggtattggc gttgcagatg cttacgcgcg cgagcaactg ctgcggcgtt ttgacgatgc
    10621 ggttggaacg ggtgtaaagc ttggcgtcgc cgccgaacat cgcggcatgg caggcgctgc
    10681 aggatttttc atggagactt ttgccgattt tcggatcggc attcgggaaa agatcggcgg
    10741 cggctggaaa aaccgcaagc agtgccaaga ggatgagccc gtatttcatg acagattcgc
    10801 ttagccggag ctgctggctg cctgcagtct tgcgatgcgg gtcgcggcag gcgggtgcga
    10861 atcgtaaaat gccgagtgca gcggatcagg cgtaagcgtg gaggcgttgt ccttgtagag
    10921 cttcaccagc gcccgcacca gatcgggagc ggaagaatac tgtactgcat attggtcggc
    10981 ctcgaattcg tgcaggcgcg aatatcgggc tagcagcggc tgcagcagga aggtgaaaat
    11041 cggcgtaatc atgaaaaaca gcaggagcgc catggcagtg gaaggtgtgg cgacgttgag
    11101 ccccgcgtaa aaccacgttt tgtccatcag cacgcccagg agccacagga aggcgaggct
    11161 tactacgaac gtccacaaca tgcgcttgat gacatggtgg cgcctgtaat ggccgagctc
    11221 gtgcgccagc accgcttcga tttccggggg ctcgaggcgc gagagcaggg tatcgaaaaa
    11281 cacgatgcgc ttggctgcgc cgaaaccggt gaagtaggcg ttgccgtggt tgctgcgctt
    11341 ggagccgtcc atcacgaaca ggccctgcga cttgaagccg cacttttcca acagacgctc
    11401 gacccgtctt ttcaattccg ggtcttccat cggcgagaat ttgttgaaga gcggtgcaat
    11461 gtagttggga taaattgcca gcaccagaat gttgaaggcg acccaggtga gccatacgta
    11521 gagccaccag tactcgccca ttttcgccat cagccacagc acgcagaaca agagcggaat
    11581 gccgaacgtg gcggcgagca gcatttgttt cagcaaatcc attacaaaca gcgcgggcgt
    11641 catctggttg aagccgaagc gcgcctcgat cacaaaagtg cgataaatgt tgagtggagt
    11701 ttccaccacg ccgacgatga tggcgacgct tacaatgagc gccatgccga aagcgagtcc
    11761 gctgccgaac aagctggccc aaagcccgct caacacctgc agtccgccgc ccagggtgag
    11821 gaccagcaac aacaccgcgt cgagaaccgc gtgtgccatg tgcagccgcg ctttggcgct
    11881 gctgtaatct gccgctttct ggtgcgccgg caggttgatt tgagtggcga attccgccgg
    11941 cacgcgctcg cggttggcct ggatatggcg aacatgccgc gacgccagcc acagctgcgt
    12001 ggtggtggtg gcaagcagtg cagccaggaa cacgacggta aaagtgtgca tgccttttcg
    12061 gctcaggtga tggaagtcaa gagcctctaa cttgataaaa cgcgtattgc gacgacatct
    12121 ttatggccgg caacgcaagc tcgttgtgtt agaagctctc gggatatgac acaataagca
    12181 ctctcgagtt gcgaagcata ttatgccaca agacgacaat cacctcatct ggattgacct
    12241 ggaaatgagc gggctcaatc cggatatcga cagagtcctc gaggtcgcgc tggtggtcac
    12301 cgattccaat ctcaacaccg tggcagaggc gccggtgctg gtggtgcgcc agccggatgc
    12361 agtgctggac tcgatggacg actggaacaa atccacccat gccaaatccg gtctgattga
    12421 caaggtcaag gcgtcgaatc tttcggaagc cgatgtcgaa gcgcagatga ttgcgttttt
    12481 gagccagtat gtgcccgcca agggttcgcc catgtgcggc aattcgatat gtcaggaccg
    12541 gcgcttcctg gcgcggtaca tgccgcaact ggaagcgtat tttcactatc gcaacctcga
    12601 cgtcagcac
//
LOCUS       DATIOW010000205         8105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_801685, whole genome shotgun sequence.
ACCESSION   DATIOW010000205 DATIOW010000000
VERSION     DATIOW010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8105
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801685"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VLL03_06890"
     CDS             complement(<1..804)
                     /locus_tag="VLL03_06890"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HSS47120.1"
                     /translation="MSRQHPVVAVTGSSGAGTSSVTLSFDHIFRREKINAVVVGGDSF
                     HRYDREEMKREIAKGEKNGQRPISHFGPEANLFEELEGLFRQYGETGTGKTRRYLHNE
                     EEAALYKRKPGTFTPWESIAEDSDLLFYEGLHGGVVTPTANVAKYADLLIGVVPIVNL
                     EWIQKIHRDMQDRGYSIEAVTQTILRRMHDYVHYIAPQFSRTHINFQRVPTVDTSNPF
                     IARDIPTADESFVVIRFRNPKGIDFPYLLTMIHDSFMSRPNCIVVPGGKM"
     gene            complement(943..1125)
                     /locus_tag="VLL03_06895"
     CDS             complement(943..1125)
                     /locus_tag="VLL03_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS47121.1"
                     /translation="MKPALLFLSLLLCCVSAIAAPVAWVEAVQMPAWLERSGVRQPLA
                     AKLKGQPGAEQPSVSR"
     gene            1213..3864
                     /gene="leuS"
                     /locus_tag="VLL03_06900"
     CDS             1213..3864
                     /gene="leuS"
                     /locus_tag="VLL03_06900"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HSS47122.1"
                     /translation="MQAQYRPEEIERQARGYWESRKAFRALEHPGKAKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLTRFYRMKSYNVLQPMGWDAFGLPAENAAIANNVPPAQWTY
                     GNIAEMKKQLQSLGFAIDWEREFATCEPKYYRWNQWLFLRMLEKGLAYKKAGVVNWDP
                     VDQTVLANEQVIDGRGWRTDALVEKREIPMYYLKITAYADELLEALEKLPGWPDRVKT
                     MQANWIGKSFGVNFGFPYELDGEKKLLRVFTTRADTIMGVTFCAVAAEHPLATRLARD
                     NPKLAAFVDECKRGSVIEAELAMMEKKGMPTGFYVTHPLTNEKIEVWVGNYVLMGYGE
                     GAVMAVPAHDERDFAFAKKYHLPIKPVIDVGGKPYSLDAWQSWYADYGVCVNSGKYNG
                     LDYQRAIDAIAADLKKMRLGEKQITYRLRDWGISRQRYWGCPIPIIHCDSCGDVPVPN
                     EQLPVVLPENLVPDGTGNPLAKTPSFYECLCPKCNKKARRETDTMDTFVDSSWYFLRY
                     ACADQNKAMVDERVSYWLPVDQYIGGIEHAILHLLYSRFWTKVMRDVGLIKIDEPFTN
                     LLTQGMVLNEIFFRKNASGYITYYNPADVEIIMDDKCQRVGVKLMANGEAVESGGIGT
                     MSKSKNNGVDPQALIKEYGADTARLFMMFASPPEQTLEWSDSGVEGASRFLRRLWQFC
                     HAQINECCAPELWAQTSLEVNWAQCSPRVRKLGYEIHSLLKQANFDYKRTQFNTVVSA
                     CMKMLNTVDDFWRQPLEPVRSGEPIYPDADRPVIKQCLSVLLRLLAPITPHVCHHLWR
                     ELGYGENILEAPWPEPDPTALVTDEIELVLQVNGKLRGNMRVPRDSAREAIEQLAVTH
                     PSVQKYVNGQPIKKVVIVPGRLVNVVV"
     gene            3963..4448
                     /gene="lptE"
                     /locus_tag="VLL03_06905"
     CDS             3963..4448
                     /gene="lptE"
                     /locus_tag="VLL03_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HSS47123.1"
                     /translation="MKKFLAVACCLLVTACGFHLRGQTTLPFETLYINASGGSAFATQ
                     LKRALLAGTNARLSDSSKDAQAIFDLVSEIQEKQILSLSGAGTVREFELRYRVAFRVH
                     DNQGRDWIPQTEITLKRDVTFNDSQVLAKESEEALLYKDMQSDAVQQVMRRLSAAKVP
                     S"
     gene            4448..5473
                     /gene="holA"
                     /locus_tag="VLL03_06910"
     CDS             4448..5473
                     /gene="holA"
                     /locus_tag="VLL03_06910"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HSS47124.1"
                     /translation="MRIEAEQLKQHLQRGLKSLYTVHGDEPLLALEAADQIRSAAKQA
                     GFAEREIFTAEPGCDWQRLAISSQSLSLFADKKLVELRIPSGKPGVEGTQAIESFCSA
                     AGPDLMGLVLLPKLDGQIKNSKWFGALQQHGEVVNASAVERGRLPQWIAGRLAVQAQN
                     ADSDTLQFIADRVEGNLLAAHQEVQKLGLLYPAGPLSFEQVRVAVLEVARYDVFKLGE
                     AMLAADAVRFARILEGLRGEGTAPPLILWALAEETRALLKIVMGLEQGRPLPQLLREA
                     RVWGARQEGMQRAVKRLRKKMLEEALLHAARLDRMIKGLASGNVWDEFTQLGLRFCSG
                     PAGVKIR"
     gene            5476..6741
                     /locus_tag="VLL03_06915"
     CDS             5476..6741
                     /locus_tag="VLL03_06915"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HSS47125.1"
                     /translation="MDIVDIKAYMHGVGREARAASRLMAKADTRTKNKALTVMAQAIR
                     REAQSLLAANAKDMEKAGAQKLDDALLDRLALSAKIVDNMAEGLMQIVQLPDPVGEIT
                     GLNYRPTGIQVGKMRVPLGVVGIIYEARPNVTADAAGLCLKAGNASILRGGSEAMHSN
                     QAIAALVREGLRAAGLPETAVQVIETADRAAVGELLSMNQYVDIIVPRGGKSLVERVM
                     RESRIPMIKHLDGVCHVYIDDKADIEKAIRIADNAKTQRYGTCNTMETLLVAEGIASQ
                     VLPQLCKIYLAKGVELRGDEKSRALVPQIKAATEEDWYTEYLAPILSVRVVKDVEQAI
                     QHIATYGSQHTDAIVTEDYGRAMRFLREVDSSSVMVNASTRFADGFEYGLGAEVGIST
                     DKIHARGPVGLEGLTSQKFIVLGDGQVRQ"
     gene            6889..>8105
                     /locus_tag="VLL03_06920"
     CDS             6889..>8105
                     /locus_tag="VLL03_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSS47126.1"
                     /translation="MNKLIEVKVPDIGDFNDVPVIEVLVKPGDQIAKEDPLITLESDK
                     ATMEIPAPQAGVVKELKIKIGDKVSEGSLILTLHTAASAPAESHKSLQAAPAVTAVKF
                     AADIKTEVVVLGAGPGGYTAAFRTADLGKQVVLIERYPNLGGVCLNVGCIPSKALLHV
                     AKVISETEEMAHQGVSFGKPKIETDKLRAWKQSVTGKLSKGLAGLARQRKVQVLQGVA
                     KFASPNMLRVETQDSVKTVSFDYCIIAAGSQAAKIPGFPDDPRILDSTSALEIQDIPQ
                     KLLVIGGGIIGLEIACVYDALGSKITVVELLDGLMLGADRDLVKPLQKRIEKRYQGIY
                     LKTKAVKIEPQKDGLKVHFDGDIAPKQQVFDRVLVAVGRQPNGKAIAAEQAGVQVTDK
                     GFIPVDKQMRTNVS"
BASE COUNT         1867 a         2243 c         2355 g         1640 t
ORIGIN      
        1 cattttgccg ccgggcacta cgatgcaatt gggccgcgac atgaaggagt cgtgtatcat
       61 cgtcagcaga tagggaaaat caatcccctt ggggttgcgg aagcggatca ccacgaagct
      121 ttcgtctgcg gtcgggatgt cgcgcgcgat gaatggattg gaggtgtcta ctgtcggcac
      181 gcgctggaag ttgatgtggg tgcgtgaaaa ctgcggcgcg atgtaatgca cgtaatcatg
      241 catgcgccgc aggatagtct gggtcaccgc ctcgatggaa taacctcgat cttgcatgtc
      301 gcggtggatt ttctgtatcc actccaggtt gacgatgggc accacgccga tcagcaaatc
      361 ggcgtatttc gccacgttcg cagtcggcgt caccacacca ccgtgcaacc cttcgtaaaa
      421 cagcaagtcg ctgtcttccg cgatcgactc ccatggggtg aaggtgccgg gctttcgctt
      481 gtacagcgcg gcttcctctt cgttgtgcag gtagcggcgg gttttcccgg tgccggtctc
      541 gccgtactgg cggaacaggc cttcgagctc ctcgaacaga ttcgcttccg ggccgaaatg
      601 gctgatggga cgttgaccgt ttttctcgcc cttggcgatt tcccgcttca tctcctcgcg
      661 gtcatagcgg tgaaagctgt cgcctcccac gaccaccgca ttgatttttt cgcggcggaa
      721 gatatggtcg aagctgaggg tgaccgagct ggtgcccgct cccgacgaac cggtgaccgc
      781 tacaaccgga tgctggcgtg acattgaggc tgtcgctccc taagttattc gaaacttcca
      841 caaagatggt acaacgaaaa accataaaca atagttatat ttttttattg ttacgataag
      901 gattgtttag gtgccggcgg aaggcggcag cccggccgga aatcagcggg ataccgaggg
      961 ttgttccgcg cccggctgcc ccttgagttt cgccgccagc ggctggcgca caccgctgcg
     1021 ctccagccac gccggcatct gtacagcttc cacccaggca accggtgccg caatcgccga
     1081 aacgcaacaa agcaacagag acaaaaagag cagcgccggc ttcatggttt ctccttaacc
     1141 ccgaattttc ggatggtatc aggtataatt ctcaagtttt ttatagtttt tataataagg
     1201 attcattcgc ttatgcaagc gcagtaccgg cctgaagaaa tcgagcggca ggcgcgagga
     1261 tattgggaat cgcgcaaggc gttccgcgca ctggaacatc cgggcaaagc caaatattac
     1321 tgcctctcca tgtttcccta tccttcgggg aagctgcaca tggggcatgt gcgcaactat
     1381 accatcggcg atgtgctcac ccgattctat cgcatgaaaa gctacaacgt actgcagccg
     1441 atgggctggg atgcgtttgg gctccctgcg gaaaacgcgg cgatagccaa taatgtcccg
     1501 ccggcccagt ggacctacgg caatatcgct gaaatgaaaa agcagctcca gtcgctgggc
     1561 ttcgccattg actgggagcg cgagtttgcc acctgcgaac ccaaatacta tcgctggaac
     1621 cagtggctgt tcttgcgcat gctggaaaaa ggcctcgcct acaaaaaagc cggagtggtg
     1681 aactgggacc cggtggacca gacggtgctc gccaacgagc aggtgatcga tggccgcggc
     1741 tggcgcaccg acgcgcttgt ggaaaaacgc gaaatcccga tgtattacct caaaatcacc
     1801 gcctacgctg acgaattgct ggaagccctg gaaaagctgc ccggctggcc ggacagggtg
     1861 aagaccatgc aggctaactg gatcggcaaa agctttggcg tcaatttcgg ctttccctac
     1921 gaactcgatg gcgagaaaaa attgctgcgc gtcttcacca cccgcgccga caccatcatg
     1981 ggggtgacct tctgcgcggt ggcggccgaa catccgctgg cgacaaggct tgcacgtgac
     2041 aatcccaagc tcgccgcgtt cgtagacgaa tgcaagcgcg gcagcgtaat cgaagccgaa
     2101 ctcgcgatga tggaaaagaa aggcatgccc accggattct atgtcaccca cccgcttacc
     2161 aatgaaaaga tcgaggtgtg ggtgggcaac tacgtgctga tgggctatgg cgaaggcgcg
     2221 gtgatggcgg tgcccgcgca tgacgagcgc gattttgctt ttgccaaaaa atatcatctt
     2281 ccaatcaaac cggtgattga tgtcggcggc aaaccgtact cgcttgatgc ctggcagtcg
     2341 tggtacgccg attatggcgt gtgtgtgaat tccggcaaat acaatggtct cgattatcag
     2401 cgggcgatcg atgccatcgc cgccgacctg aaaaaaatga ggctgggcga gaagcagatc
     2461 acctaccgcc tgcgcgattg gggcatttca cgccagcgct actggggctg cccgattccg
     2521 ataattcact gcgattcctg cggcgacgtg ccggtgccga atgagcaatt gccggtcgtt
     2581 ttgccggaaa acctggttcc cgatggcacc ggcaatccgc tcgccaagac gccttcgttt
     2641 tacgaatgtc tgtgtccgaa gtgcaataaa aaagccagac gcgagaccga cactatggac
     2701 acctttgtgg attcgtcgtg gtatttcctg cgttacgctt gcgccgacca gaataaagcg
     2761 atggtggacg agcgtgtttc ctactggttg ccggtggacc agtacatcgg cggcatcgag
     2821 cacgccatcc tgcatctgct ctattcgcgc ttctggacca aggtcatgcg cgatgtggga
     2881 ctcatcaaaa tcgacgagcc tttcaccaac ctgcttaccc agggcatggt gctgaatgaa
     2941 atcttcttcc gcaagaatgc aagcggctac attacttatt acaatccagc cgatgtggaa
     3001 atcatcatgg acgacaaatg ccagcgggtg ggcgtcaagc ttatggcgaa tggcgaggca
     3061 gtggaatcgg gtggcatcgg caccatgtcg aaatcgaaga acaacggcgt tgatccgcaa
     3121 gcgctgatta aagaatacgg cgccgacacg gcgcggctgt tcatgatgtt tgcttcgcca
     3181 cccgagcaga cgctggagtg gtcggacagt ggtgtcgaag gcgcgtcgcg cttcttgagg
     3241 cggctttggc agttttgtca tgctcaaatc aacgaatgct gcgcgcccga gctttgggca
     3301 caaacatctc tcgaagtgaa ttgggctcaa tgttcgccgc gcgttcgcaa gcttggttac
     3361 gaaatccatt ccttgctgaa gcaagcgaat ttcgattaca aaagaaccca gttcaacacg
     3421 gttgtttcgg catgcatgaa gatgctaaac actgtggacg acttttggcg gcaaccatta
     3481 gaaccagttc gttcgggtga acctatttac cctgacgccg accgacctgt aattaaacaa
     3541 tgtctctcag ttctgctgcg tctcctcgcg ccgatcacgc cgcatgtgtg ccatcatttg
     3601 tggcgcgaac tcggctacgg cgagaatatt ctcgaggcac cgtggccgga acccgatccc
     3661 acggcacttg tcaccgacga aatcgaactg gtgctgcagg taaacggtaa gctgcgcggc
     3721 aacatgcgcg tgccccgcga ttccgcgcgc gaggccattg agcagctggc ggtgacccac
     3781 cccagcgtgc aaaaatatgt gaacggccag cccatcaaga aagtggtgat cgtgccggga
     3841 cggctggtca acgtggtggt atgagctagt gaacagtaaa ctgcgaacgg tgaacagaaa
     3901 gccggccggg gcttccgata actgtttacc gatcaccgtt cacccgtcaa gcggagtgaa
     3961 acatgaagaa atttcttgcc gttgcttgtt gcctgttggt tacggcttgc ggctttcact
     4021 tgcgcgggca gaccacgctg ccgtttgaaa ccttgtatat caacgcttcc ggcggttccg
     4081 cttttgccac gcaactgaag cgcgcgctgc tggccggcac caacgcccgc ctgagcgaca
     4141 gctccaagga tgcgcaagct attttcgatc tggtgagcga aatccaggag aaacaaatct
     4201 tgagcttgag cggcgccggc accgtgcgcg aattcgagtt gcgctatcgc gtggctttcc
     4261 gcgtgcatga caaccaaggc cgcgactgga taccacagac cgaaatcacc ctcaagcgcg
     4321 acgtcacgtt caacgactcg caagtactgg ccaaggaatc cgaagaagcg ttgctttata
     4381 aagacatgca gagcgatgcc gtgcaacagg tcatgcggcg cttgagcgcg gccaaagtgc
     4441 catcctgatg cgcatcgaag cggaacagct caaacagcat ttacaacggg gtctgaaatc
     4501 cctctacacg gttcatggcg acgagccgct gttggccctt gaagccgcgg accaaatacg
     4561 cagcgcggca aaacaggcgg gcttcgccga acgggaaatc ttcaccgccg agccggggtg
     4621 cgattggcag cggctcgcca ttagcagcca aagcctttcg ctcttcgccg acaaaaagct
     4681 cgttgaactg cgcatccctt caggcaagcc gggagttgaa ggcacgcagg caatcgaaag
     4741 cttctgttct gccgctggcc ccgatctgat ggggctggtg ctgctgccca agctcgacgg
     4801 gcaaataaaa aactcgaaat ggtttggggc gttgcagcag catggcgaag tggtgaacgc
     4861 cagcgccgtg gaacgcgggc ggctgccgca atggatagca ggacgcctcg ctgtgcaagc
     4921 acagaacgcc gattccgata cgctgcaatt catcgccgac cgcgtcgaag gcaatctgct
     4981 cgccgcgcac caggaagtgc agaaactggg gttgctctac cccgccgggc cattgagctt
     5041 tgagcaagtc agagtggcag tactggaagt cgcgcgctac gatgtgttca agctgggcga
     5101 ggcgatgctc gccgcggatg ccgtgcgctt cgcgcgcatt ctcgagggcc tgcgtggtga
     5161 aggcactgcg ccgcctctga tactctgggc gctagcggaa gaaactcgtg cgctgcttaa
     5221 aatcgtgatg ggcctcgaac aaggcaggcc gctgccgcaa ttgctgcgcg aagcgcgggt
     5281 gtggggggcg cgccaggaag gaatgcagcg cgcagtgaaa cgcctgcgca aaaaaatgct
     5341 ggaagaagcg cttttgcacg cggcaaggct ggaccgcatg atcaagggac tggcttccgg
     5401 caatgtgtgg gacgagttta cgcaattggg cttgcgtttc tgttccggcc cggccggggt
     5461 taaaatacgc taatcatgga tatcgttgac atcaaagcct acatgcacgg cgtggggcgc
     5521 gaagcgcgcg ccgcctcccg cctcatggcc aaagccgaca cgcggaccaa gaacaaggcg
     5581 ttaaccgtca tggcacaggc aattcggcgt gaagcgcaaa gcctgcttgc ggccaacgcc
     5641 aaggacatgg aaaaagcagg cgctcagaag ctggatgacg cgctgctcga ccgcctcgcg
     5701 ctttcggcca aaatcgtgga caacatggcc gaagggctga tgcaaatcgt gcagctgccc
     5761 gacccggtgg gcgaaatcac cggcctcaat taccgtccta ccggaattca ggtagggaaa
     5821 atgcgtgtgc cgctgggggt ggtcggcatt atttacgaag cgcgccccaa cgttaccgcg
     5881 gatgccgccg ggctttgcct caaagccggc aacgcttcca tcctgcgcgg cggctctgag
     5941 gcgatgcatt ccaaccaagc gatcgcggcg ctggtgcgcg aaggtttgcg cgccgctggg
     6001 ctgccggaaa ccgcggtgca ggtaattgaa accgccgacc gcgccgcggt gggcgagctg
     6061 ctgtcgatga atcagtacgt cgacatcatc gtgccgcgtg gcggtaagag cttagtcgag
     6121 cgcgtgatgc gcgaatcgcg catccccatg atcaagcacc tggacggcgt ctgccatgtc
     6181 tacatcgacg acaaagcgga catcgaaaaa gcaatccgca ttgccgacaa cgccaagacg
     6241 cagcgctacg gtacctgcaa caccatggag acgctgctgg ttgcggaagg cattgcttca
     6301 caagtgctgc cgcagctttg caagatttat ctagccaaag gcgtggagct cagaggcgac
     6361 gagaaatcac gtgcgctggt gccacaaatc aaggccgcca ccgaagaaga ctggtacacc
     6421 gaatacctcg cgccgatttt gagcgtgcgc gtggtcaaag acgtggagca ggctattcag
     6481 cacatcgcca cctacggctc gcagcacacc gacgccatcg tgacggaaga ttacggccgc
     6541 gccatgcgtt tcctgcgcga agtggattcg agctcggtga tggtgaacgc ctcgacgcgc
     6601 ttcgccgacg gcttcgaata tggcttgggc gcggaagttg gaatttccac cgacaaaatc
     6661 cacgcacgcg gcccggttgg gctggaaggg ctcacgtcgc agaagttcat cgtcctcggc
     6721 gacggtcaag tccggcaata aacttcaacc cactaaaaaa actactagac gggactaaca
     6781 ggatttttca gaatttacat gatttatcct gttaatctgt tgaaatcttg taaatcctgt
     6841 ccgttgtttt agattttctc tttgcctatg tggttaattt aggcttctat gaacaagctt
     6901 atcgaagtca aagttccgga tatcggcgat ttcaacgacg tcccggtgat cgaagtcctg
     6961 gtgaaacccg gagaccaaat cgccaaggaa gatccgctga tcacgctgga atcggacaaa
     7021 gcgaccatgg aaattcccgc cccgcaagcg ggagtggtga aagagctcaa aatcaaaatc
     7081 ggcgacaagg tgtcagaagg ttcgctgatt ctcacgctgc acaccgcagc ttccgcaccc
     7141 gctgaatccc acaaatcact tcaagccgca cccgctgtta cggcggtcaa attcgctgcc
     7201 gatattaaaa ctgaagtcgt ggtgctaggc gccggcccgg gcggctatac tgcggcgttc
     7261 cgcactgccg atctcggcaa gcaagtggta ctgatcgaac gctatcccaa tctgggcgga
     7321 gtgtgcctca atgttggctg cattccttcg aaagcattgc tgcacgtggc caaggtcatc
     7381 agtgaaacgg aggaaatggc gcatcaaggc gtgagtttcg gcaaacccaa aatcgaaacc
     7441 gacaaacttc gcgcctggaa gcaaagcgtg actggcaagc tgagcaaggg cctggcgggg
     7501 cttgccaggc agcgcaaggt gcaggtattg caaggcgtgg ccaagtttgc ctcgcccaat
     7561 atgctgcgtg tggaaacgca ggatagcgtc aaaaccgtgt ctttcgatta ctgcatcatt
     7621 gccgcgggat cgcaagcggc aaagattccc ggttttcccg acgacccgcg catcttggat
     7681 tcaacgagcg cgctggaaat tcaggacatc ccgcaaaaat tgcttgtaat aggcggaggc
     7741 atcatcggcc tggaaatcgc gtgcgtatac gacgcgctgg gctcgaaaat cactgtagtg
     7801 gaactgctcg atggattgat gctcggcgct gatcgcgatc ttgttaagcc tctgcaaaaa
     7861 cgcattgaaa agcgttacca gggaatttat ctcaaaacca aagctgtaaa aatcgaaccg
     7921 caaaaggacg gattaaaagt tcattttgac ggcgacattg cgccgaagca gcaggtattc
     7981 gaccgcgtac tggtagcggt tggtagacaa cccaacggca aagccatagc cgcagagcaa
     8041 gctggtgttc aggtaaccga caaaggtttt attccggtgg acaagcagat gcgcaccaat
     8101 gtgtc
//
LOCUS       DATIOW010000206         5303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1206785, whole genome shotgun sequence.
ACCESSION   DATIOW010000206 DATIOW010000000
VERSION     DATIOW010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206785"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1481)
                     /locus_tag="VLL03_06925"
     CDS             complement(<1..1481)
                     /locus_tag="VLL03_06925"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSS47127.1"
                     /translation="MLGFSLALMLIAVNIFVSHLAYQNTERLITAHEEAAQLLNSLRV
                     SGEVLQLLTDAETGQRGYLITGDERYLEPYNAALLGIGDKLKQLRSTIPMPKSKDLAK
                     LESLVSAEVAVLNETVVVRGKIGFEAAQKVVLTNRGKTAMDSIRRLIGGIEQEERLLM
                     EQRDAATQTISRQLFFTYAIAALLNLVLLAVFFYIGYSDLRESAQDKRALRDLNEMLQ
                     GGMRELTRRNRESTLLSEMSEVLQSCLEPKEAYHAIAQFGRQFFPGEAGVFFVMHDSH
                     NYLENVATWGEPEIGGPLFAPEECWALRRGQLHRVDDARTGLQCEHVKAAGAPRLHYM
                     CVPMVVHSETLGLLHLQFGTESSPEEAAQRETAAELLAATFADQIALAIANLRLRDSL
                     RQQSIHDPLTGLHNRRYLEEALTRELARAERKKTPLAVIILDVDHFKRFNDTFGHEAG
                     DAVLRSLAQLIGRQIRGSDVACRFGGEEFVLVLPEADLNIARQR"
     gene            complement(1546..2391)
                     /gene="rsmA"
                     /locus_tag="VLL03_06930"
     CDS             complement(1546..2391)
                     /gene="rsmA"
                     /locus_tag="VLL03_06930"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HSS47128.1"
                     /translation="MDKNARTGKAPAEYDTEKLHAAGLMQHFPRKRFGQHFLIDEQIV
                     STIIAAISPQGADSIVEIGPGLGALTRPLLARLNHLHVIEIDRDIVARLTQEFSAERL
                     TIHNSDALRFDFARLGENLRVVGNLPYNISSPLMFHLTRFAQRIRDLHLMLQKEVVER
                     MAAVPSTAAYGRLSVMLQYRYNMEQLLVVPSNAFRPAPKVESAVIRLIPRATHELQAK
                     DEELFAKIVTRAFTQRRKTLRNALMLHLASQDFEKLDLDPGLRAENLSSDDFVRISNY
                     LGKQD"
     gene            complement(2354..3373)
                     /gene="pdxA"
                     /locus_tag="VLL03_06935"
     CDS             complement(2354..3373)
                     /gene="pdxA"
                     /locus_tag="VLL03_06935"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015572650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HSS47129.1"
                     /translation="MPRTTSLPTIAVTAGEPAGIGPDLCTLVAGHKLPARIVIIADKT
                     LLVQRTGLLKKKIKLVDFSEQAKAAAPGNLYVLHEPLKEPARPGKLNPTNSAYVLRTL
                     DRAIWGCLSGQFDAMVTAPVQKSVINDAGIVFTGHTEYLAEKTGTPEVVMMLCGGGLR
                     VALATTHLALKDVPAALTQEGLERVLRILHRDLVRRFGIAAPRILVAGLNPHAGESGY
                     LGTEESRLISPVVKKLCYEGFDVQGPLPADTLFTPERLNRCDAVLVMYHDQGLPVLKY
                     ASFGAGVNVTLGLPIIRTSVDHGTALDLAGTGKIDCGSFIAAIKLALQLVKNGQKRQN
                     RKSAG"
     gene            complement(3435..4766)
                     /locus_tag="VLL03_06940"
     CDS             complement(3435..4766)
                     /locus_tag="VLL03_06940"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSS47130.1"
                     /translation="MSTHKTFPRVAAFAAFAAAFAVAPLGAAQTDPKSQKVVPLDRIV
                     AVVNDDVITQYDLNDNIKLVVNQLNKQGTPLPPQEVLEKQILERLVNDRVQLQFAKET
                     GVRVDDTQLEKTLERIAEGNGMTLQLMREKLEKESINFNRFREEIRSEIIMTRLKERE
                     VDSKINVTESEIDNFLRTQEALSDRNDEYELAHILVLVPEQASPEQIQAKRARAEQAL
                     LQLKNGADFAQIAAGYSDAPDALQGGILGWRSTGRLPGIFVEALRAMQVGEVSPILRS
                     ANGFHLVKLINKRGSDAAKAIEQTHARHILIKTSELVSEPEARNRLANLKERLENGAD
                     FGELARLHSEDGSAPKGGDLGWLSPGDTVPDFERAMHALKPGQISEPVHSQFGWHLIQ
                     VLERRNQDITKDQQRLLARQTLRARKSDEAHEEWLRQLRDRAYVEYRLEDK"
     gene            complement(4797..>5303)
                     /gene="lptD"
                     /locus_tag="VLL03_06945"
     CDS             complement(4797..>5303)
                     /gene="lptD"
                     /locus_tag="VLL03_06945"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HSS47131.1"
                     /translation="PSVDTNSDILASISSRITPMLLAEAGLQYHPSSSTGQKVGFGLR
                     YQPEPGKVLNLSYRFIRDSIEQTDISAQWPLGGGWYGLGRFTYSLRDSTTLEGLLGFE
                     YNSCCWTLRLVAHRLTTATSKATTGVFLQLELNGLSKLGSNPLTVLRQDIPGYTKTNQ
                     PGHAGGLY"
BASE COUNT         1000 a         1497 c         1617 g         1189 t
ORIGIN      
        1 cgctggcgcg cgatgtttaa atccgcttcc ggcaaaacca gaacgaattc ctccccgcca
       61 aaacggcagg cgacgtcgct gccgcggatt tgccgcccga tcagttgcgc caggctgcgc
      121 agcaccgcat cgcccgcttc atgaccgaag gtgtcgttga agcgcttgaa gtgatccacg
      181 tcgaggatga tgaccgccag cggggtcttt ttgcgctcgg cgcgggcgag ttcgcgggtg
      241 agtgcttctt caaggtagcg ccggttgtgc agcccggtga gcggatcgtg aatggattgc
      301 tggcgcagtg aatcgcgcag ccgcagattg gcgatggcga gcgcgatctg gtcggcaaac
      361 gtcgcggcca gcagctccgc cgcggtttcc cgctgcgccg cctcctcagg gctcgattcg
      421 gtgccgaatt gcagatgcaa aagaccaagg gtttcggaat gcacgaccat gggcacgcac
      481 atgtaatgca gccggggagc gcccgccgct ttcacgtgct cgcactgcaa tccggtgcgc
      541 gcatcgtcca cccggtgcaa ctgcccgcgg cgcagtgccc agcattcttc cggcgcgaac
      601 agtgggccgc cgatctcggg ctcgccccag gtcgccacgt tttcaaggta attgtgcgaa
      661 tcgtgcatga cgaaaaacac gcccgcctcg ccgggaaaaa attgtcttcc gaactgcgcg
      721 atggcgtgat acgcctcctt tggttcgagg caggattgca aaacttcgct catttcgctc
      781 agcaaagtgc tttcgcggtt gcgccgcgta agctcgcgca ttcccccttg cagcatttcg
      841 ttgaggtccc gcagagcccg cttgtcctgc gcgctttccc gcagatcgct gtaaccgatg
      901 tagaaaaaca ctgctaacag caccaggttg agcagcgccg ctatggcata ggtgaaaaag
      961 agttggcgcg agatggtttg ggtggccgcg tcgcgttgct ccatcagcag gcgctcctcc
     1021 tgctcgattc cgccgataag gcggcgaatg ctgtccatcg cggttttgcc tctgttggta
     1081 agcaccactt tttgcgcggc ttcgaaaccg attttcccgc gtaccacgac ggtttcgttg
     1141 agtacggcga cttcggcgct taccagagat tcaagcttgg cgagatcctt ggatttcggc
     1201 atcgggatgg tcgaacgcag ttgcttaagc ttgtcgccga tgccgagaag ggcagcgtta
     1261 taaggctcga gatagcgttc atcgccggtg ataaggtagc cgcgctgtcc ggtttcggcg
     1321 tcggtgagca gctgcaatac ttcgcccgaa accctgaggc tattcagcaa ttgtgctgct
     1381 tcttcgtgtg cggtgataag ccgctcggtg ttctgataag cgaggtggga aacgaagatg
     1441 ttgacggcaa tcaacatcag cgccaggcta aatccaagca agcttctttt agagaaaaac
     1501 caattcatgt ggttgtggaa gcttgtggct gaatgttaga ggttcttaat cttgtttacc
     1561 aaggtaattg ctgatgcgca caaaatcgtc gctggacagg ttttcagcac gcaaaccggg
     1621 atcgaggtca agcttttcga agtcctggga ggcaagatgg agcataaggg cgttgcgcaa
     1681 ggttttcctg cgctgggtaa atgcgcgggt cacgattttg gcgaaaagtt cttcatcttt
     1741 tgcctgcagt tcatgcgttg cgcgggggat gagacggatc acggcggact cgaccttggg
     1801 cgccgggcgg aaggcgttgc ttggcacgac cagcagctgc tccatgttgt agcggtattg
     1861 tagcatcacc gataaccggc cataggccgc agtcgacggc acggcggcca tgcgctctac
     1921 cacttccttt tgcagcatga gatgcaaatc gcgtatgcgc tgtgcgaagc gggtaagatg
     1981 aaacatgagc ggcgaggaaa tgttgtaggg aagattgccg accacgcgca aattttcgcc
     2041 cagacgggca aaatcgaagc gcaaggcatc gctgttgtgt atcgtgagcc gttccgcaga
     2101 aaattcttgg gttaagcgcg ccacgatgtc gcggtcgatt tctatgacgt ggagatggtt
     2161 aagacgcgcc agcagcgggc gcgtaagcgc ccccagccct ggaccaattt ctacgataga
     2221 atctgcgcct tgtggagaaa tcgcagcaat gatggtggat acgatttgct cgtcaatcag
     2281 gaagtgctga ccaaaacgtt ttcttgggaa gtgttgcatt aaaccggccg catggagttt
     2341 ttccgtgtcg tattcagccg gcgcttttcc tgttctggcg tttttgtcca ttcttcacca
     2401 gctgcaacgc cagcttgatg gcggcaatga agctgccgca gtcgattttt ccggtccctg
     2461 ccagatcgag ggcggtgcca tggtcaactg aagtgcggat gatgggcaag ccgagcgtga
     2521 cgttgacccc ggcgccgaag cttgcatatt tcaacaccgg cagaccctgg tcgtggtaca
     2581 tcaccagcac cgcgtcgcag cggtttaacc gctcgggcgt gaacagggtg tccgcgggca
     2641 gcggtccctg cacatcaaaa ccttcgtagc agagcttttt caccaccggg ctgatgaggc
     2701 gcgattcctc ggttcccaga taaccggatt cgccggcatg cgggttcaag cccgccacca
     2761 gtatgcgcgg cgcggcgatg ccgaaacgcc gcaccaggtc ccggtgcaga atgcgcaaca
     2821 cgcgctccag tccttcttgg gtcagtgccg ccggcacgtc tttcaaagcc aggtgggtgg
     2881 tggcgagcgc cacccgcaag ccgccgccgc agagcatcat caccacttcg ggtgtgccgg
     2941 ttttttcagc gagatattcg gtatgcccgg taaagacgat gccggcatcg ttaattacgc
     3001 tcttttgcac cggcgcggtc accatggcgt cgaattgtcc gctgaggcag ccccatatcg
     3061 cccggtccag cgtgcgcagt acataagcgc tgttggtcgg attgagtttc cccgggcgcg
     3121 cgggctcctt gagcggctcg tgcagcacat acaggtttcc aggcgcggct gctttcgctt
     3181 gctcactgaa atccaccagt ttgatttttt tcttgagcag tccggtgcgc tggacgagaa
     3241 gagttttatc cgcgatgatg acgatgcggg ccggcaactt gtggcccgct accagcgtgc
     3301 acaaatcggg gccgatgccc gccggctcgc ctgcagtcac ggcaatggtg ggcaacgatg
     3361 tggtgcgagg catggattaa acgcagagac gaacgcagag tttgatgcgg atgtcccttc
     3421 gcctctgcgg tgatttattt atcttccaac cggtattcca cgtaagcgcg gtcgcgcaac
     3481 tggcgcagcc attcttcgtg ggcttcgtcc gacttgcggg cgcgcaaggt ctggcgcgca
     3541 agcaggcgct gctgatcctt ggtgatgtcc tgattgcgcc gctccagtac ctgaatcaga
     3601 tgccagccga attgcgagtg caccggctcg ctgatttggc cgggcttcaa cgcatgcatt
     3661 gcccgttcga aatctggcac cgtgtcgccg ggagacaacc agccgaggtc gccgcccttg
     3721 ggtgcgctgc cgtcttcgga atgcaaccgc gccaattccc caaagtccgc gccgttttcc
     3781 aggcgctcct tgaggttagc caggcggttg cgcgcttccg gctccgacac cagctcgctg
     3841 gttttgatca gtatgtgacg cgcgtgggtt tgctcaatgg cttttgcggc gtcgctgccg
     3901 cgcttgttga tgagcttgac gaggtgaaag ccgttggcgc tgcgcaggat cgggctgacc
     3961 tcgccgacct gcatcgcgcg cagcgcttcc acgaaaatgc cgggcaaacg cccggtggaa
     4021 cgccagccga gtatgccgcc ttgaagcgca tcgggcgcat cggagtaacc cgccgcaatc
     4081 tgcgcgaaat ccgcgccgtt cttaagctgc aaaagcgcct gttcggcgcg cgcgcgtttg
     4141 gcttggattt gttccgggct ggcctgttcc ggcaccagca ccaggatgtg cgcgagttcg
     4201 tattcgtcgt tgcggtcgga aagcgcttcc tgagtcctca agaaattgtc gatttcgctc
     4261 tcggtgacgt tgattttgct gtccacctcg cgttctttga ggcgggtcat gatgatttca
     4321 gaacgaattt cctcgcggaa gcggttaaag ttgatgcttt ccttttccag tttttcgcgc
     4381 ataagctgca gggtcatgcc attgccctcg gcgatgcgct ccagggtttt ctccagctgc
     4441 gtgtcgtcca cgcgcacgcc ggtttccttg gcgaattgca gttgcacgcg gtcatttact
     4501 aggcgctcga gaatttgttt ttccagtacc tcctgcggcg gcagcggcgt gccctgcttg
     4561 ttaagctggt tcaccaccag cttgatgttg tcgttgagat cgtattgggt aatcacatcg
     4621 tcgttcacga ccgcgacgat gcgatcgagc ggcaccactt tttgcgattt cgggtcggtc
     4681 tgcgccgcgc cgagcggtgc aaccgcaaac gcggcggcaa acgccgcgaa ggcggcaacg
     4741 cgcggaaaag ttttgtgagt gctcatctac actgcgtatc ggatggtatg aagcgcctag
     4801 tacagtccac cggcatgacc cggctggttg gtcttggtgt agccgggaat gtcctgcctt
     4861 aaaactgtga gaggattaga gccaagcttc gagagaccgt tcaactcaag ctggagaaac
     4921 acgccggtgg tcgccttcga cgtggcggtg gtgagacgat gggccaccaa gcgcagcgtc
     4981 cagcagcagc tattatattc aaatccgagc agtccttcca aagttgtgct gtcgcgcagc
     5041 gagtaagtga agcgccccag cccgtaccat cctccgccga gcggccattg cgcgctgata
     5101 tccgtctgct cgatggaatc gcggatgaag cggtagctca agttcaatac cttgcccggt
     5161 tccggctggt agcgcaagcc gaagccgact ttctgtccgg tactggaaga agggtggtat
     5221 tgtagcccgg cttctgccag caacatgggg gttatcctgc tgctgataga ggcgaggata
     5281 tccgaattgg tgtcgacact ggg
//
LOCUS       DATIOW010000207         1865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1715438, whole genome shotgun sequence.
ACCESSION   DATIOW010000207 DATIOW010000000
VERSION     DATIOW010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1715438"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VLL03_06950"
     CDS             complement(<1..440)
                     /locus_tag="VLL03_06950"
                     /inference="COORDINATES: protein motif:HMM:NF037184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HSS47132.1"
                     /translation="MPQLIKLRGRNALSGFRLNKLLSSLKTTVSTIAGIHAEFWHFAV
                     LSRELTDAETSVLQRILTYGPAVGADKISGELLLVVPRIGTVSPWSSKATDIARQCGL
                     AAIERIERGVAFYLETREALSAHDKQALQPLIHDRMTESVLESF"
     gene            515..>1865
                     /locus_tag="VLL03_06955"
     CDS             515..>1865
                     /locus_tag="VLL03_06955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HSS47133.1"
                     /translation="MIEDATAPRPIYLTRDIRKIEARFLPVHRLMEAAGLAAAELARE
                     LLGAGGTSVLVIAGPGNNGGDALVLARHLKQWWFKVDVVFAGNAKKLPRDAARALRLW
                     QEDGGELHSKIPAGKWDLVVDGLFGIGLKRRLEGRYAQLVERINRLGVPVLALDVPSG
                     LDADTGRTLGTAVRATHTVTFIALKPGLLTADGVNCSGEIHVRSLDIDAAASLPPKGW
                     LLSQVTRPRLRPRLPGSHKGMFGNAGILGGAAGMVGAALLAGRAALKLGAGRTYVALL
                     AENAPLVDPMQPELMLRTPEELLDLDHLDCLAVGPGLGQSNEAQHFLAAALKTKLPLV
                     LDADALNLIGFEALLQQLLKKRSAPTILTPHPAEAGRLLKFGTEEIQHDRLAAALEIA
                     RRFRCHAVLKGAGSICALPDGSWYINSSGNPGMASAGMGDVLTGMIAALIAQGLSSGD
                     "
BASE COUNT          388 a          537 c          565 g          375 t
ORIGIN      
        1 tcgaaagact ccagcaccga ttcggtcatc cggtcgtgaa tcaacggctg cagtgcctgt
       61 ttatcatgcg ctgacaacgc ctcgcgcgtt tccagataaa aagcgacgcc acgctcgatg
      121 cgctcgattg ctgccaggcc gcattgacgg gcgatgtcgg tggccttgga tgaccaggga
      181 gaaacagtgc cgatgcgcgg caccaccagc aacagctcgc cgctgatctt gtccgcgccc
      241 actgccgggc cataggttaa aatccgctgc aggacagaag tctccgcgtc ggtcaactct
      301 ctcgacagca ccgcgaaatg ccaaaattcc gcgtgtatcc cggcaatcgt ggataccgtg
      361 gttttcaagg acgaaagcag tttgttgaga cggaaaccag aaagggcgtt gcgcccgcgc
      421 agcttgatga gttggggcat cagttgcgat caacttctaa aagtcgaatt ataccctaac
      481 cagccgaccg ctttgcagct aaactatcat caccatgatt gaagacgcga cagcgccaag
      541 gccgatttac ctgactcgcg acatcaggaa aatcgaagcc aggtttttgc ctgtacaccg
      601 tctgatggaa gcggcaggcc tcgccgccgc cgagctcgcg cgcgagctgc tgggtgcggg
      661 cggcaccagc gtgctggtta ttgccgggcc gggcaacaac ggcggtgatg ccctggttct
      721 tgcacgacat ctcaagcagt ggtggttcaa ggttgatgtc gtgttcgccg ggaatgcaaa
      781 gaaattgccc cgcgacgccg cccgcgcttt gcgcctgtgg caagaggacg ggggggaatt
      841 gcacagcaaa attcccgcag gcaaatggga cctggtggtg gacggtttgt tcggcattgg
      901 actcaagcgc cggctcgaag gccgctacgc gcaacttgtc gagcgcatca atcggcttgg
      961 cgttccggtg ctggcgctgg atgtaccgag cgggcttgac gccgataccg gtcgtactct
     1021 gggaactgca gtccgcgcta ctcacactgt aaccttcatt gccttgaagc ctggattgct
     1081 cactgcggac ggcgtgaact gcagtggaga aattcacgtg cgtagtcttg atattgacgc
     1141 cgcggcttcc ctgcccccca aaggatggct gctttcacaa gtaactcgcc cgcggctcag
     1201 gccgcgcctc cccggcagcc acaaaggaat gtttggcaac gcgggcatac ttggcggtgc
     1261 agcaggcatg gtgggcgcgg cgctgctcgc cggccgcgct gcactgaagc tgggcgccgg
     1321 caggacttat gtcgcactcc tcgctgaaaa cgcgcctttg gttgatccaa tgcagcctga
     1381 actcatgctg cgtacgcccg aagaattgct tgatcttgat catctcgatt gcctggcggt
     1441 cggaccgggc ttgggacaat cgaatgaagc gcagcacttt cttgctgccg cactcaagac
     1501 aaaattgccg ctggtgttgg atgcagacgc gttgaacctg attggtttcg aagcgctgtt
     1561 gcaacaattg ttgaagaaac gctccgcgcc gacgattctc acgccccatc ccgccgaagc
     1621 ggggcgcttg cttaagttcg gcaccgaaga aatccagcac gaccgcctgg ccgccgcgct
     1681 ggaaatcgcg cgacgcttcc gctgccatgc cgtgctgaaa ggcgcaggaa gcatttgcgc
     1741 attaccagat ggaagctggt atatcaattc aagcggcaat cctggaatgg caagcgcagg
     1801 catgggcgat gtgttgacgg ggatgatagc tgcgctgatt gcacaagggt tgagctcagg
     1861 cgatg
//
LOCUS       DATIOW010000208         1990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1207286, whole genome shotgun sequence.
ACCESSION   DATIOW010000208 DATIOW010000000
VERSION     DATIOW010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207286"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1990)
                     /gene="pnp"
                     /locus_tag="VLL03_06960"
     CDS             complement(<1..>1990)
                     /gene="pnp"
                     /locus_tag="VLL03_06960"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HSS47134.1"
                     /translation="QQVTLETGEIARQATGAVLVSIEDTVVLVTIVAAKETKPGQDFF
                     PLTVDYQERTYAAGKIPGGFFKREGRPSEKETLTSRLIDRPLRPLFPEGFYNEVQIVA
                     TVVSSNNEVDSDIPAMLGASAAMAISGIPFNGPIGAARVGYVNGEYVLNPTATQLKSS
                     SLNLVIAGTEQAVLMVESEALELPEDIMLGAVVFGHRQMQEAIKAINELADEAGTPLW
                     DYQHPVKDEALIARVTELAEADLHQAYDIRQKQARGQQINAIRSRVLSELNGENPDPE
                     RENLIKTIFMDVEAKIVRNQILNGEPRIDGRDTRAVRPITIRTGALPRAHGSALFTRG
                     ETQAMVVATLGTARDGQIVDALQGEYTDRFILHYNMPPYATGETGRVGSPKRREIGHG
                     RLAKRALIGVLPTVEEFGYSMRVVSEITESNGSSSMASVCGGCLALMDAGVPIKAHVA
                     GIAMGLIKEGNHFAVLTDILGDEDYLGDMDFKVAGTEKGVTALQMDIKIQGITKEIMR
                     VALNQAREGRMHILELMKQVLPSARGEISAYAPRIMTIKIKPEKIRDVIGKGGAVIRA
                     LTEETGTTIDIAEDGTVTIACTASEGCLLAKKRIEDITADVEVGRVYEGTVLKLLDFG
                     AIVSVLPGKDGLLHISQIANERVNSVADHLREGQTVKVK"
BASE COUNT          369 a          593 c          555 g          473 t
ORIGIN      
        1 tttcacttta accgtctgcc cttctctaag gtggtccgcc acgctgttga cgcgctcgtt
       61 ggcaatttgc gagatgtgca gcaatccgtc cttgcccggc agcacgctca cgatggcgcc
      121 aaaatccagg agcttgagca ctgtaccttc ataaacccgg ccgacctcca catccgcagt
      181 gatgtcctca atgcgcttct tagccagcag acagccttca gaggcggtac aggcaatagt
      241 caccgtgcca tcttcggcga tatcaatggt tgtcccggtt tcttcggtaa gcgcgcggat
      301 caccgcacca cccttgccga tgacatcgcg gattttctcc ggcttaatct tgatggtcat
      361 gatgcgcggc gcgtaagccg aaatttcacc gcgcgcgctg ggcagcacct gtttcatcaa
      421 ctccagaata tgcatgcgcc cttcgcgcgc ctggttgaga gcgacgcgca tgatttcctt
      481 ggtgatgccc tgaatcttga tatccatttg cagtgcggtg acgccctttt cagtgcccgc
      541 caccttgaag tccatgtccc cgagatagtc ctcatcaccc agaatgtcgg tgagtaccgc
      601 gaagtggttg ccttccttga taaggcccat agcgatgccc gcgacatgcg ccttgatagg
      661 aacgccggcg tccatcagcg caagacagcc gccgcacacc gaggccattg aactcgagcc
      721 gtttgattcg gtgatttccg acaccacacg catggaatag ccaaattcct ccacagtcgg
      781 caacaccccg atcaatgcgc gcttcgccag ccgcccatgg ccgatttcgc ggcgcttggg
      841 cgaaccgaca cggccggttt caccggttgc ataaggcggc atgttgtaat ggagtatgaa
      901 gcggtctgtg tattcgcctt gcagcgcatc tacaatctgc ccatcgcgtg cagtgccgag
      961 cgtcgccacc accattgcct gggtttctcc gcgggtaaac agtgccgagc catgagcgcg
     1021 cggcaatgcg ccggtgcgga tggtgatagg ccgtaccgcg cgggtgtcgc gaccgtctat
     1081 gcgcggctcg ccgttgagaa tctggttgcg cacgattttc gcttccacat ccatgaaaat
     1141 ggttttaatc aggttctcgc gctcaggatc gggattttcg ccattgagtt cgcttaaaac
     1201 ccggctgcgg attgcgttta tctgctggcc gcgcgcttgc ttttgcctga tgtcgtacgc
     1261 ctggtgcaaa tcggcttccg cgagttcggt gactctcgct atcagcgctt cgtccttgac
     1321 cgggtgttga tagtcccaaa gcggcgtgcc cgcttcgtca gcaagttcgt tgatggcttt
     1381 gatcgcctcc tgcatttggc ggtgcccgaa caccaccgcg ccgagcatga tgtcttcggg
     1441 cagttccagc gcttcggatt cgaccatcag cactgcttgt tcagtgccgg cgatcaccag
     1501 gttgagggac gaagatttta attgggtggc ggtggggttt aatacgtatt cgccgttgac
     1561 atatcctacc cgtgctgcgc cgatcggtcc gttaaaggga atcccggaaa tcgccattgc
     1621 tgccgaagca cccagcatcg ccggaatgtc ggaatcaacc tcgttgttgg aagacaccac
     1681 ggtagcaacg atttgcacct cgttgtaaaa accttccgga aacaacgggc gcaaggggcg
     1741 gtcaatcaag cgcgatgtta gggtttcctt ttctgagggc cggccttcgc gcttaaaaaa
     1801 accgccgggt attttcccgg cggcgtaggt gcgttcctgg taatcgacgg tgagcgggaa
     1861 gaagtcctgt cctggtttgg tctctttggc ggccactata gtgaccagca ccaccgtgtc
     1921 ctctatgctg accagcacgg cgccggtcgc ctggcgggcg atttctccgg tttccagcgt
     1981 tacttgttgc
//
LOCUS       DATIOW010000209         1841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1614994, whole genome shotgun sequence.
ACCESSION   DATIOW010000209 DATIOW010000000
VERSION     DATIOW010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614994"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..>1841)
                     /locus_tag="VLL03_06965"
     CDS             complement(18..>1841)
                     /locus_tag="VLL03_06965"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HSS47135.1"
                     /translation="MHYVPLQHTPAVLRYLAGAGLTTREACGNTIRNVTACPMAGICP
                     REHTDVTKHLDGAVEHFLRNPLSQQLPRKFKISFSGCEADCAQGMLHCMGIVAVKRGD
                     RFGFKVLAGGGLGHKPHEAIVVEEFIEEKDLLHSMEAIVTMHNKYSDRTKRAKSRIKF
                     LVDRFGPAGFIEKYREELARIKQALANQPYPKGEWKTGGEGEICGQGAPRHVVPQKQP
                     GLFVLPVSTPIGNLNAKQLHGLAKALQEHGLDEIRTTQDQNLMVINVPQAKLDSLRKT
                     IAALGLREPEAGDNVVACPGTSTCRLGITSSTLLGPRLRNAVGGDLRIRVSGCHNGCA
                     QPEMGDIGIYGEGKRMHGKLIPHYQTYFGGNGLAGGGLAIKGPSVPAARIESAVQRVR
                     VAFDKDHLPDESFFFWTRRRGIGYFNELLADLVQVPETEVGSVLRDHGDNQDFKVLQL
                     GGGECAGASQMLVGSNFFEAAHERGYRNALVFQRKYGEAVKCAEAIARLIGQGVVHLL
                     GGNNKQESLADLGAELVGLLSDQPELGEQLAEFGAAFARDSEEWTEKELNPMFAKLDS
                     WTMQVAELCVSRDRQLDLSDALPRPLAAHKAIPTAAQAAAA"
BASE COUNT          332 a          572 c          543 g          394 t
ORIGIN      
        1 tgttgcgggt aatcatgtca ggccgcggct gcctgcgctg cggtgggaat tgctttgtgc
       61 gcggcaagcg ggcgcggcag cgcgtctgaa agatcgagct ggcggtcgcg gctgacacac
      121 aattcggcga cttgcatggt ccagctatcc agcttggcga acatggggtt gagttctttt
      181 tccgtccact cctcggaatc gcgcgcgaac gcagcaccaa actccgccag ttgctcgccc
      241 agttcgggct ggtcagacaa caggccgaca agttcggcgc cgagatcggc aaggctctcc
      301 tgcttgttgt tgccgcccag caagtgcacc acgccctgcc caatcaagcg ggcaatcgct
      361 tcagcgcatt tcaccgcctc gccgtacttg cgctggaaca ccagtgcatt gcggtagccg
      421 cgctcatgcg ccgcctcaaa gaagttcgaa ccgaccagca tctgcgaagc gccggcgcat
      481 tccccgccac cgagttgcaa gaccttgaag tcctgattat cgccgtggtc gcgcagcacc
      541 gagccaactt ccgtttccgg cacttgcacc aaatcggcaa gcagctcgtt gaaataaccg
      601 atgccgcggc gccgcgtcca gaagaaaaaa ctttcatccg gcaggtgatc cttgtcgaaa
      661 gccacgcgca ctcgctgcac cgcactctcg atgcgcgccg cgggcaccga aggtccttta
      721 atcgcgagtc ctccaccagc cagtccatta ccgccgaaat aagtctggta atgcggaatt
      781 aatttcccat gcatacgctt gccttcgccg taaatgccga tatcgcccat ctccggctgg
      841 gcgcagccgt tgtggcagcc cgagacgcga atgcgcaaat cgccgccgac ggcgttcctg
      901 agcctggggc cgagcagcgt ggatgaggtg atgccgaggc ggcaggtgga agtgccaggg
      961 caggccacca cgttgtcgcc ggcttccggc tcgcgcagtc cgagcgcggc aatcgtcttg
     1021 cgcagcgagt cgagtttcgc ttggggcaca tttatcacca tcaggttctg gtcctgggtg
     1081 gtgcggattt catccaagcc atgctcttgc agcgccttcg ccagaccatg caactgcttc
     1141 gcgttcaagt tccctatagg cgtgctaact ggcagcacaa aaagcccggg ttgtttttgc
     1201 ggcaccacat ggcgtggcgc gccctgtccg cagatttcgc cttcacctcc ggttttccat
     1261 tcccctttgg gataaggctg gttggcgagc gcttgtttta tccgtgccag ctcctcgcgg
     1321 tatttttcga tgaagcctgc cggcccgaaa cggtccacca ggaacttgat gcgcgatttg
     1381 gcgcgcttgg tgcgatcgga atacttgttg tgcatggtga cgatggcttc catcgagtgc
     1441 agcaaatctt tttcctcgat gaattcttcc accacgatgg cttcgtgcgg tttgtggccg
     1501 agcccgcctc ccgccagcac cttgaagccg aagcggtcgc ctcttttcac cgcgacgata
     1561 cccatgcaat gcagcatgcc ttgcgcgcag tcggcctcgc aacctgagaa gctgattttg
     1621 aatttgcgcg gcagctgctg gctgagggga ttcctcagga aatgttcgac cgcgccgtcc
     1681 agatgtttgg tcacatcggt atgttcgcgc gggcagattc cggccatggg acaggcggtg
     1741 acgttgcgta tggtgttgcc gcaggcttcg cgcgtggtga gccccgcacc ggccaaataa
     1801 cgtagcacag cgggcgtgtg ctgcagcggc acgtagtgca t
//
LOCUS       DATIOW010000210         6889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_802719, whole genome shotgun sequence.
ACCESSION   DATIOW010000210 DATIOW010000000
VERSION     DATIOW010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802719"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..154
                     /gene="tuf"
                     /locus_tag="VLL03_06970"
     CDS             <1..154
                     /gene="tuf"
                     /locus_tag="VLL03_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286074.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HSS47136.1"
                     /translation="AKGTEMVMPGDNVSVTVQLIAPIAMEEGLRFAIREGGRTVGAGV
                     VAKIIE"
     gene            160..468
                     /gene="rpsJ"
                     /locus_tag="VLL03_06975"
     CDS             160..468
                     /gene="rpsJ"
                     /locus_tag="VLL03_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HSS47137.1"
                     /translation="MQSQKIRIRLKAFDYRLIDQSALEIVETAKRTGAVVRGPVPLPT
                     RIERFDILRSPHADKTSRDQFEIRTHLRLMDIIDPTDKTVDALMKLDLPAGVDVEIKL
                     "
     gene            570..1214
                     /gene="rplC"
                     /locus_tag="VLL03_06980"
     CDS             570..1214
                     /gene="rplC"
                     /locus_tag="VLL03_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HSS47138.1"
                     /translation="MSLGLVGRKVGMTRIFTDDGDTLPVTVLDVSNNRITQIKTPGSD
                     GYAAVQVTCGKRRASRVNKAAAGHFAKAAVEAGHMLREFKVTQDELANLKVGASIGVD
                     IFKVGQKVDVTGTSQGKGFAGVIKRHHFRSNRASHGNSISHNKPGSTGQAQDPGRVFP
                     GKRMAGQLGNVKRTAQNLEVLRVDAERQLLLVKGALPGAKGADVIVRPAVKARA"
     gene            1214..1831
                     /gene="rplD"
                     /locus_tag="VLL03_06985"
     CDS             1214..1831
                     /gene="rplD"
                     /locus_tag="VLL03_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HSS47139.1"
                     /translation="MELKLITEKGKASTIKASDDLFGREYNEALVHQIITAYLANARQ
                     GTRAQKGRSDINKSHKKPWRQKGTGRARAGMASSPLWRGGGKIFPNLPDENFTQKVNR
                     KMYRAGMCSIFSQLAREGRISVVESFMLEAPKTKLLVQKLKGLGLEDVMIITDNLDEN
                     LYLSSRNLPNVRVVKPGQADPVSLIRHRNVLLTKAAVQKIEEMLA"
     gene            1828..2154
                     /gene="rplW"
                     /locus_tag="VLL03_06990"
     CDS             1828..2154
                     /gene="rplW"
                     /locus_tag="VLL03_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HSS47140.1"
                     /translation="MSAFNAERLTQVIIAPQVSEKSTMVGDKHEQVVFRVAADASKPE
                     IKAAVEQMFSTKDKKIEVLSVQVVNVKGKDKRFGRFTGRRKNWKKAYVCLKPGQEINF
                     AAVESK"
     gene            2154..2987
                     /gene="rplB"
                     /locus_tag="VLL03_06995"
     CDS             2154..2987
                     /gene="rplB"
                     /locus_tag="VLL03_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HSS47141.1"
                     /translation="MALIKVKPTSPGRRVLLKVVSPELHKGAPYAPLLVKQTRKAGRN
                     NRGHITTRHQGGGHKQHYRSIDFRRDKDGIPAKVERLEYDPNRSAHLALLCYADGERR
                     YIVAPKGLAVGAQVVNGPEAPIKTGNALPLRNIPVGTTVHCVEMLPGKGAQLARAAGT
                     SVQLLAREGSYAQLRLRSGEIRKVHIECRAAIGEVGNEENNLRQIGKAGAQRWRGIRP
                     TVRGVAMNPIDHPHGGGEGRTAAAQPPVSPWGVQCKGFKTRRNKRTAGMIVRRRHKVK
                     G"
     gene            2990..3289
                     /gene="rpsS"
                     /locus_tag="VLL03_07000"
     CDS             2990..3289
                     /gene="rpsS"
                     /locus_tag="VLL03_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HSS47142.1"
                     /translation="MARSVKKGPFVDHHLLKRVEAAQATKDKRPIKTWSRRSTILPDF
                     VGLTFAVHNGKQHVPVYVSENMVGHKLGEFSHTRIFKGHSAVGDKKTAATEEVKK"
     gene            3299..3631
                     /gene="rplV"
                     /locus_tag="VLL03_07005"
     CDS             3299..3631
                     /gene="rplV"
                     /locus_tag="VLL03_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HSS47143.1"
                     /translation="MKTSAALRGVRLSAQKGRLVADQIRGLSVDKALNILSFSPKKGA
                     AIIKKALESAIANAEHNDGADIDELKVTTICVERGSSLKRFHARAKGRGNRITKPTCH
                     IYVTVGDK"
     gene            3641..4555
                     /gene="rpsC"
                     /locus_tag="VLL03_07010"
     CDS             3641..4555
                     /gene="rpsC"
                     /locus_tag="VLL03_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HSS47144.1"
                     /translation="MGQKIHPTGFRLSVQRNWSSRWYANSKNFPAMLNEDIKVREFLQ
                     QKLSHAAVGKVLIERPAKNARITIYSARPGVVIGKKGEDIESLKTQLQKLMNIPVHVN
                     IEEIRKPELDARLIADNIAQQLQKRIMFRRAMKRAITNAMRLGAQGIKIMSSGRLNGI
                     EIARSEWYREGRVPLHTLRADIDYGFAEAKTTYGVIGIKVWVFKGEMLGHADQPVAAL
                     PVETEKRTKPSKTEDEGRRGTPREAKPKAKPAEERKAAEPAKLKAKTAKRETKPKTEV
                     ESRAKPKAKAAAGKAKPKVKVKVKKSGA"
     gene            4557..4970
                     /gene="rplP"
                     /locus_tag="VLL03_07015"
     CDS             4557..4970
                     /gene="rplP"
                     /locus_tag="VLL03_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011901907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HSS47145.1"
                     /translation="MLQPARTKYRKQQKGRNKGIATRGNKVNFGEFGLKAVTRGRLTS
                     RQIEAARRAMTHHIKRGGRIWIRIFPDKPVSQKPAEVRMGNGKGNPEFWVSEIKPGAV
                     LYEMDGVDEPMAREAFRLAAAKLPILTTFVIRQVG"
     gene            4974..5168
                     /gene="rpmC"
                     /locus_tag="VLL03_07020"
     CDS             4974..5168
                     /gene="rpmC"
                     /locus_tag="VLL03_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HSS47146.1"
                     /translation="MKTDELRAKSAAELKQELNQLLKAQFGLRMQRATQQLTNFSQLR
                     KVRRDIARVRTVLTEKTRQS"
     gene            5165..5428
                     /gene="rpsQ"
                     /locus_tag="VLL03_07025"
     CDS             5165..5428
                     /gene="rpsQ"
                     /locus_tag="VLL03_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HSS47147.1"
                     /translation="MSETANKRTLSGRVVSDKMNKTVTVLVERTVRHPRYGKIITRSK
                     KYHAHDEKSEYHEGDVVIIEECRPIAKTKAWQVTRLVRKLRSL"
     gene            5574..5942
                     /gene="rplN"
                     /locus_tag="VLL03_07030"
     CDS             5574..5942
                     /gene="rplN"
                     /locus_tag="VLL03_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HSS47148.1"
                     /translation="MIQMQTVLDVADNTGARSVMCIKVLGGSKRRYAGIGDIIKVSVK
                     DAVPRGRVKKGEIYHAVVVRTAKGVRRLDGSLIKFDGNAAVLLDPKLEPIGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            5952..6275
                     /gene="rplX"
                     /locus_tag="VLL03_07035"
     CDS             5952..6275
                     /gene="rplX"
                     /locus_tag="VLL03_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HSS47149.1"
                     /translation="MQKIKKGDEVVVITGKDKGKRGAVLRKVDAEHVVVESVNKVKKH
                     QRPNPLKNTTGGIIDKEMPIHISNVMLFNPTTRKGDRVGVKVLDDGRRVRYFKSNGEM
                     VDVKA"
     gene            6285..6824
                     /gene="rplE"
                     /locus_tag="VLL03_07040"
     CDS             6285..6824
                     /gene="rplE"
                     /locus_tag="VLL03_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HSS47150.1"
                     /translation="MARLQTYYKDTVVKQLMKQFGYKSEMQVPRITKITLNMGVGEAV
                     ADKKIMDNAVGDMQKIVGQKPVVTKSKKSIAGYKIRKGYPIGCMVTLRSTRMYEFLDR
                     LVSIAIPRIRDFRGLSAKSFDGRGNYNMGVKEQIIFPEIEYDKIDALRGLNITITTTA
                     KSDDEARALLAAFKFPFKS"
BASE COUNT         1676 a         1948 c         2146 g         1119 t
ORIGIN      
        1 ggccaagggg accgagatgg tgatgccggg ggacaacgtc tccgtcacgg tgcagttgat
       61 tgccccgatt gcgatggagg agggcttgcg ctttgccatt cgcgaaggcg gccgcaccgt
      121 gggcgccggc gtggtggcga aaattattga gtgatggaca tgcaaagcca aaaaatccgc
      181 atccgtttga aagcgttcga ttaccggctg attgaccaat cggcgctgga aatcgtcgag
      241 acggcgaaac gcaccggcgc ggtggtgcgc ggccccgtgc cgctgcctac gcgcatcgaa
      301 cgctttgaca tattgcgcag cccgcacgcc gacaagacgt cgcgcgacca gtttgaaatc
      361 cgcacgcact tgcgcttgat ggacatcatc gatcccaccg acaagacggt ggatgcgctg
      421 atgaagctgg atctgcctgc aggcgtggat gtggagatta agttgtgacg tgtgacaggt
      481 aacgagtgac gcgtcacttg tcacccgtca ccagttactg attttattgc cggtcaattg
      541 caaccggcgc cttaaaaaag gtgaagaaaa tgagtttagg attggtcggc cgcaaggttg
      601 gcatgacccg gatttttacc gatgacggcg ataccctgcc ggtgacggtg ctggacgtgt
      661 ccaacaaccg cattacccaa atcaagaccc cgggcagcga cggttatgcc gcggtgcagg
      721 taacctgcgg caagcggcgc gcgagccggg tcaataaagc cgcggccggg catttcgcca
      781 aagccgccgt tgaagccggc catatgctgc gcgaattcaa agtcacgcaa gatgagctcg
      841 ccaacctgaa agtcggcgct agcatcggcg ttgacatttt caaagtcggg cagaaagtgg
      901 atgtcaccgg caccagccag ggcaagggct ttgccggcgt gatcaagcgc catcacttca
      961 gatccaaccg ggcgagccat ggcaattcga tttcccacaa caaacccggc tccaccggtc
     1021 aggcgcagga cccgggccgc gtgtttcccg gcaagcgcat ggcgggccag ctcggcaatg
     1081 tgaagcgcac cgcgcaaaac ctggaagtgc tgcgcgttga tgccgagcgg cagctgctct
     1141 tggtcaaggg cgcgctgccg ggcgccaagg gggccgacgt gattgtgcgt cccgcggtga
     1201 aagcgagggc ctgatggaac tcaaactgat tacagagaaa ggtaaagcct ccacgatcaa
     1261 ggcgtcggac gatttgttcg ggcgcgaata caacgaggcg ctggtgcacc agatcatcac
     1321 cgcctatctc gccaacgcgc ggcaaggcac gcgggcgcag aaagggcgca gcgacatcaa
     1381 taaatcgcac aagaagccgt ggcgccagaa aggcaccggc cgtgcccgcg ccggcatggc
     1441 ttccagcccg ttgtggcgcg gcggcggcaa gattttcccc aatctgcccg acgagaattt
     1501 tacccagaag gttaacagga agatgtaccg agccggcatg tgttcgatat tttcgcaact
     1561 ggcgcgcgaa ggacgcatct cggtggtgga aagtttcatg ctggaagcgc cgaagaccaa
     1621 gcttttggtg caaaagctca agggcctggg cctggaggat gtgatgatca tcaccgacaa
     1681 tctggacgag aacctttatc tctcatcgcg caacctgccc aacgtccgcg tggtcaagcc
     1741 cgggcaggcg gatccggtga gcctgatccg ccaccgcaat gtgctgttga ccaaggccgc
     1801 ggtgcagaaa atcgaggaga tgctggcatg agcgcattca atgccgaacg cctgacgcag
     1861 gttattatcg caccgcaggt atcggaaaag agcaccatgg tcggcgacaa gcacgaacag
     1921 gtggtgttcc gcgtggcggc cgacgccagc aaaccggaaa tcaaggctgc ggtcgagcag
     1981 atgttcagca ccaaggacaa gaaaatcgaa gtgctctcgg tacaggtggt caacgtcaag
     2041 ggcaaggaca agcgcttcgg ccggtttacc ggtcggcgca agaactggaa aaaagcctat
     2101 gtctgcctca agccggggca ggaaatcaat ttcgcggcag tggagagtaa ataatggcac
     2161 tgatcaaggt taaacccact tctcccggcc ggcgcgtcct actcaaggtg gtgagcccgg
     2221 agctgcacaa aggcgcgccc tatgcgccgc tgctggtcaa gcagaccagg aaagccggcc
     2281 gcaacaaccg cggccacatc accacccgcc atcagggcgg cgggcacaag cagcattacc
     2341 gcagcatcga tttccgccgc gacaaggacg gcatcccggc caaagtggag cgcctggaat
     2401 acgatccgaa tcggagcgcg catttggcgc ttctgtgtta cgccgacggc gagcgccgtt
     2461 acatcgttgc gcccaagggt ctcgccgtcg gtgcgcaagt ggtgaacgga ccggaggcgc
     2521 cgatcaagac cggcaatgcg ctgccactca ggaacatccc ggtcggcact acagtgcact
     2581 gcgtggaaat gctgcccggc aagggcgcgc aactcgcgcg cgccgcgggt acttcggtgc
     2641 aattactggc gcgtgaaggc agctacgcac aattgcggct gcgctccggt gaaatccgca
     2701 aggtgcacat cgagtgccgc gcagccatcg gcgaagtcgg caacgaggaa aataatttgc
     2761 gtcagatcgg taaggccggt gcgcagcgct ggcgcggcat acgtccgacg gtgcgcggcg
     2821 tggcgatgaa cccgattgac catccgcacg ggggcggcga gggccgcacc gccgcggcgc
     2881 agccgccggt gagtccgtgg ggtgtgcaat gcaagggatt caagacccgc cgcaacaagc
     2941 gcaccgccgg catgatcgtg cgccgccgtc ataaggtgaa ggggtaacta tggcacgttc
     3001 tgttaaaaaa ggcccgtttg tagaccatca tctgctgaaa agggtggaag cggcgcaagc
     3061 caccaaggac aagcgcccga tcaaaacctg gtcgcggcgc tcgaccatcc ttccggattt
     3121 cgtgggactc actttcgcgg tgcataacgg caagcagcat gtgccggtgt atgtgtcgga
     3181 aaacatggtt gggcacaaac ttggcgaatt ttcgcacacc cgcattttca aggggcactc
     3241 cgccgtcggc gacaagaaaa ccgccgcgac ggaagaagtc aagaagtagg agagcggcat
     3301 gaaaacatct gcagcattgc gaggcgtaag gctttcagcg caaaaaggcc ggttggtggc
     3361 ggaccagatc cgcggcctgt cggtggacaa ggcgctcaac atcctcagct tcagcccgaa
     3421 aaaaggcgcg gcgatcatca agaaagcgct cgagtccgcc atcgccaacg ccgagcataa
     3481 cgacggcgcg gacatcgacg aactcaaagt gaccaccatt tgtgtggagc gcgggtcgag
     3541 cttgaagcgc ttccatgcgc gcgccaaagg ccggggcaat cgcatcacca aacccacctg
     3601 ccacatttat gtgacggtcg gcgacaaata aggaaacacc atgggacaga agatacatcc
     3661 gaccggtttc cgcctttccg tgcagcgcaa ctggagttcg cgctggtatg cgaacagcaa
     3721 gaatttcccg gcgatgctca atgaagacat caaggtccgc gaattcctgc aacagaagtt
     3781 gagccatgcc gccgtcggca aggtgctgat cgagcgcccg gcgaagaacg cgcgcattac
     3841 catctacagc gcccgtcccg gcgttgtcat cggcaagaag ggcgaggata tcgagtcgct
     3901 gaaaacccag ttgcaaaagc tcatgaacat cccggtgcat gtcaatatcg aggaaatccg
     3961 caaacccgag ctcgatgcgc gactgatagc cgataacatt gcgcagcaac tgcaaaagcg
     4021 catcatgttc cgccgcgcta tgaagcgcgc catcaccaac gccatgcgcc tgggcgcgca
     4081 gggcatcaag atcatgagct cggggcgctt gaacggcatt gagatcgcgc gctccgaatg
     4141 gtaccgcgaa gggcgggtgc cgctgcacac gctgcgcgcc gacatcgatt acggttttgc
     4201 cgaagccaag accacttatg gtgtgatcgg gatcaaggtc tgggtgttta agggcgagat
     4261 gctgggacat gctgatcagc cggtggccgc gctgccggtg gaaacggaaa agagaaccaa
     4321 gccgtcgaag acggaggatg aaggccgacg tggtacacca cgcgaagcca agcccaaggc
     4381 gaaacccgct gaagagcgca aggcggctga acccgccaaa ctgaaggcga agacggccaa
     4441 gcgcgaaacc aagccgaaaa ccgaagttga aagcagggct aagcccaaag ccaaggcggc
     4501 ggcaggcaaa gccaagccga aggtcaaggt caaagtgaag aagtcaggag cataagatgc
     4561 tgcaacccgc aaggaccaaa tatcgcaaac agcagaaggg ccgaaacaag ggcatcgcca
     4621 cgcgcggcaa caaagtgaac tttggcgaat ttggcctcaa agcagtgacc cgcggccgtc
     4681 tgacctcgcg ccagatcgag gcggcgcggc gcgccatgac ccaccacatt aagcgcggcg
     4741 ggcgcatctg gatccgcatt ttcccggaca agccggtttc gcagaaaccg gcggaagtac
     4801 gcatgggcaa cggcaagggt aacccggagt tctgggtgtc cgaaatcaag ccgggcgcgg
     4861 tactctacga gatggatggc gttgacgagc ccatggcgcg cgaagcgttc cgcctcgccg
     4921 cggccaagct cccgattctg accacatttg tgattcgaca ggtcggttaa cccatgaaaa
     4981 cggacgaact gagagccaag tcggcggcgg agctcaagca ggagcttaac caactcctta
     5041 aagcgcaatt cggcctgcgc atgcagagag ccacgcagca gctcaccaat ttcagccagt
     5101 tgcgcaaggt gcgccgggac atcgcgcggg tgcgcaccgt gctaaccgag aaaacgaggc
     5161 agtcatgagc gaaacagcga acaaacgcac cttgagcggc cgcgtcgtca gcgacaaaat
     5221 gaacaagacc gtgaccgtgc tggtcgagcg cactgtgcgc cacccccggt acggcaaaat
     5281 catcacgcgc tccaagaaat accacgctca cgacgagaag agcgagtacc acgagggcga
     5341 tgtggtgata atcgaggaat gcaggccgat cgccaagacc aaagcgtggc aggtgaccag
     5401 gctggttaga aaattgcgca gtctttagct tgctttcgcg caaggcggta attataattg
     5461 acagttttcg atttgctctg cggcttacgc ggggtaatcg acttcctgac gggatccaag
     5521 actgaccgtc atgccgcgca agcggcggcg gtttaagttg gaaagatgaa aaaatgattc
     5581 aaatgcagac ggtgctggac gtcgcggaca ataccggtgc gcgctcggtg atgtgcatca
     5641 aggtgctggg cggctcgaag cgccgctacg cgggcatcgg cgacatcatc aaggtgagcg
     5701 tcaaggacgc ggtgccgcgc ggccgggtga aaaagggcga gatttaccac gcggtggtgg
     5761 tgcgcaccgc caagggcgtg cgccggctgg acggatcgct gattaaattc gacggcaacg
     5821 ccgcggtgtt gctggatccc aagctcgagc ctatcggcac acgcattttc ggcccggtaa
     5881 cgcgggaact ccggaccgag cgcttcatga aaatcgtctc gctcgcgccg gaagtgctgt
     5941 aaggaaagac catgcagaaa attaaaaaag gcgacgaagt ggtagtgatc accggcaagg
     6001 acaagggcaa gcgcggcgcc gtgctgcgca aggtggatgc cgagcacgtg gtggtggaaa
     6061 gcgtgaacaa agtgaagaaa caccagcgtc ccaacccgct caagaacacc accggcggca
     6121 ttatcgacaa ggaaatgccg attcatattt ccaacgtgat gctgttcaac cccaccaccc
     6181 gcaagggcga ccgggtcggc gtcaaggtgc tggacgacgg gcgccgcgtg cgctacttca
     6241 agtccaacgg cgaaatggtc gacgtcaagg cttaacagga atccatggcg cgtttgcaaa
     6301 cttattataa ggataccgtg gtcaagcagc tgatgaagca gtttggctac aaatcggaga
     6361 tgcaggtgcc gcgcatcacc aagatcacgc tcaatatggg cgtgggcgaa gcggtggcgg
     6421 acaagaaaat catggacaac gcggtgggcg acatgcagaa aatcgtgggg caaaaaccgg
     6481 tggtgaccaa atcgaagaag tccatcgccg gctacaagat ccgcaaaggt tatcccatcg
     6541 gttgcatggt gacgctgcgg agcacgcgca tgtacgaatt tctcgaccgc ctggtttcca
     6601 tcgccatccc gcgcatccgc gacttccgcg ggctgtcggc caaatcgttt gacggtcgag
     6661 gcaattacaa catgggcgtg aaggaacaga ttattttccc ggagatcgag tacgacaaga
     6721 tcgacgcgct gcgcggcctc aacattacga tcaccaccac cgccaagtcc gacgacgagg
     6781 cgcgagcgtt gctggcggca ttcaaattcc cgttcaagag ctgaggccgg tatggcaaaa
     6841 acttccgtag tcaaccgtga actgaagcgc cgcgccgtgg tgaagcaat
//
LOCUS       DATIOW010000211         3124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_90092, whole genome shotgun sequence.
ACCESSION   DATIOW010000211 DATIOW010000000
VERSION     DATIOW010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90092"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..156
                     /locus_tag="VLL03_07045"
     CDS             <1..156
                     /locus_tag="VLL03_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS47151.1"
                     /translation="ADAKQVAESLSRALTNKFLHIPTHVLNHAHAEDREQLVALINRL
                     YQIHLQE"
     gene            153..1235
                     /gene="prfA"
                     /locus_tag="VLL03_07050"
     CDS             153..1235
                     /gene="prfA"
                     /locus_tag="VLL03_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HSS47152.1"
                     /translation="MKQSIAGKLSQLGNRLQQLNQILSSENATADLESYRKLAREHAE
                     ITPVVKLYEAYQSSERDLQAAQEMQSDPKMREFAETEIREGREKLARIESELQKKLLP
                     QDPNDERNIFLEIRAGTGGDESALFAKDLFRMYARFAERQRWQVEIISESPSEMGGYK
                     EIIAKIVGQGAYSKLKFESGGHRVQRVPETETQGRIHTSACTVAVLPEVDEISDVVLN
                     SAELRIDTYRASGAGGQHVNKTDSAVRVTHLPTGIVVECQDGRSQHKNKAQALAILAA
                     RIKDKQLREQQAKQAATRKSLIGSGDRSERIRTYNFPQGRVTDHRINLTLYKIDQIMD
                     GDMNELTGALMAEHQAELLAAVAEET"
     gene            1235..2110
                     /gene="prmC"
                     /locus_tag="VLL03_07055"
     CDS             1235..2110
                     /gene="prmC"
                     /locus_tag="VLL03_07055"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HSS47153.1"
                     /translation="MQVAVKNFASVARPVSVARALRESGLQACDANLLLQAALGVDRA
                     YLVAHHDQILSPRETTLFEELVQRRVTGEPTAYILGEREFYGLIFKIAPVVLIPRPET
                     ELLIELALERMPQEQSCKVLELGTGSGNIAVILARHRPLACITATDCSEGALAVARQN
                     AETLLGAENRCIVFKKSDWFDALDGETYDLIISNPPYVASGDAHLRQGDVPFEPRQAL
                     SGGADGLDCIRRIIRGASSRLRAGSWLLFEHGYDQAHACLELLAQSGFTRLSCSKDLA
                     GIPRVSGGQIDGGVS"
     gene            2143..2448
                     /gene="grxD"
                     /locus_tag="VLL03_07060"
     CDS             2143..2448
                     /gene="grxD"
                     /locus_tag="VLL03_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HSS47154.1"
                     /translation="MSAQELIKKQVTEHPVVLYMKGTPDFPQCGFSANVVNILKACGV
                     QDLFTVDVLSNPEVRQGIKEYANWPTIPQLYVNGEFVGGSDILSEMYQSGELQKMFK"
     gene            2531..>3124
                     /locus_tag="VLL03_07065"
     CDS             2531..>3124
                     /locus_tag="VLL03_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HSS47155.1"
                     /translation="MKIKAAVLNQMGVKPPYSASRPLAVEEVELDPPGPGEVLVKISA
                     AGLCHSDLSAINGERPRPTPLVLGHEAAGIVEESGADVCDLRKGDHVVMVFVPSCGHC
                     LPCAEGRPGLCEPGAAANVAGTLLSGARRLKRGATPLNHHMGVSAFAQYATVSRRSLV
                     KIDPDLPLDEAALFGCAVLTGVGAVLNTARVAPGSSVA"
BASE COUNT          673 a          903 c          953 g          595 t
ORIGIN      
        1 gcggatgcca agcaggtggc ggaatcctta agccgcgcgc tgaccaataa attcctgcat
       61 attcccactc atgtgctgaa tcatgcgcac gctgaagacc gcgaacaact ggtggccttg
      121 atcaaccgcc tttatcagat acacctacaa gaatgaagca aagcatcgcc ggcaagcttt
      181 cccaactcgg caaccgcctc caacaactca accaaatcct aagcagcgaa aacgccaccg
      241 cggacctgga aagttaccgc aagctcgccc gcgagcatgc ggagatcacg ccggtggtta
      301 aattgtacga ggcctatcaa tcaagcgagc gcgacctgca ggcggcgcag gaaatgcagt
      361 ccgacccgaa aatgcgcgaa ttcgcggaga ccgaaatccg cgagggacgc gagaaactcg
      421 cgcgcatcga atccgagttg caaaagaaat tgctgccgca ggaccccaat gacgaacgta
      481 acatttttct ggaaatccgt gccggcaccg gcggcgacga atcggcgctg tttgccaaag
      541 acctgttccg catgtacgcg cgcttcgcag agcggcagcg ctggcaagtg gaaatcattt
      601 ccgaaagccc ctcggagatg ggcggttaca aggaaatcat cgccaagatc gtcggccagg
      661 gtgcctattc caagctgaag ttcgaatccg gcgggcaccg tgtgcagcgc gtgccggaaa
      721 ccgaaacgca ggggcgcatc catacttccg cctgcacggt ggcggtgctt cccgaagtcg
      781 atgaaatttc cgatgtggtg ctcaattctg cggaactgcg catagacacc taccgcgctt
      841 caggtgcggg agggcagcat gtcaacaaaa ccgattccgc ggtgcgggtg acgcatttgc
      901 cgaccggcat cgtggtggaa tgccaggacg gccgctcgca gcataaaaac aaggcgcagg
      961 cgctggcgat tcttgctgcg cgcatcaaag acaagcaact gcgcgaacaa caggccaaac
     1021 aggccgcgac ccgcaaatcg ctgattggca gcggcgaccg ttccgagcgc atccgtacct
     1081 ataattttcc tcaggggcgg gtgaccgacc atcgcatcaa tctcaccttg tacaagattg
     1141 atcagatcat ggatggtgac atgaacgaac tcaccggcgc gctgatggcg gagcatcaag
     1201 ccgagctgct ggcggctgtg gccgaggaaa cctgatgcag gtggccgtca aaaattttgc
     1261 ttcagttgcc aggccggtca gcgttgcacg cgcgttgcgc gagagcgggc tgcaagcctg
     1321 cgacgcgaat ctgctgctgc aagcggcgct gggagtggac cgtgcttatc tagtcgccca
     1381 ccacgatcaa atcttgagcc cccgcgaaac gactttgttc gaggagctgg tacaacgccg
     1441 cgtgaccgga gagcccactg cttacatctt gggtgaacgg gaattttacg gactgatttt
     1501 caaaattgcc ccggtggtgc tgataccccg gcccgaaacc gagttgctga ttgaactggc
     1561 gcttgaacgc atgccgcaag agcaatcctg caaggtgctg gagctgggca ccggcagcgg
     1621 caacatcgcg gtcattcttg cccggcatcg cccactggcg tgcattaccg caacggattg
     1681 ctccgagggg gcgcttgctg tagcgcggca aaatgccgaa acactgctcg gtgcggagaa
     1741 ccggtgtatt gtttttaaaa aaagcgactg gttcgacgct ttggacgggg aaacatacga
     1801 cctcattatt tccaatcccc cttatgtcgc ttctggcgat gcgcacttgc ggcaaggcga
     1861 tgtgcccttt gagcccaggc aggcacttag cggcggagcc gacgggctcg actgcattcg
     1921 ccgtattatt cgaggagcgt cgtcgcgcct gcgcgcaggc agctggttgc tgtttgagca
     1981 tggctacgat caggcgcatg cctgcctgga attgcttgcg caaagcgggt ttacccggct
     2041 ttcttgcagc aaggacctgg cgggaattcc gcgtgtttca ggcggtcaga ttgacggtgg
     2101 ggtgtcataa tatctaaact tgttctattt tcaggagcgg ttatgagcgc gcaggaactt
     2161 atcaagaagc aggtcaccga gcaccccgtg gtgctgtaca tgaaaggcac gccggatttt
     2221 ccgcagtgcg gattcagcgc caatgtggtg aatatcctca aggcctgcgg ggtgcaggat
     2281 cttttcacgg tggatgtgct ctccaatccg gaagtccgcc agggcatcaa ggaatacgcc
     2341 aactggccga ccatcccgca gctctacgtc aacggcgaat tcgtcggcgg ctcggacatc
     2401 ctcagcgaga tgtatcaaag cggcgagctg cagaagatgt ttaaataatt ttcatcaaac
     2461 gcaaaaaagc cggcgtggcc ggcttttttg tccgcccggc tggccattca ggaatccagg
     2521 gagattgctc atgaaaatta aagccgcggt gctgaaccag atgggagtca agccgcctta
     2581 ctcggcatcc aggccgctgg cggtggagga agtcgagctc gatccgcccg gtccgggcga
     2641 agtgctggtg aaaatctccg ccgcaggttt gtgccattcc gatctctcgg ccatcaacgg
     2701 cgaacggccg cgccccacgc ccctggtgct cggccacgaa gcagccggga tcgtggaaga
     2761 gtcgggcgca gatgtgtgcg atctcaggaa aggcgatcac gtggtgatgg tgttcgtgcc
     2821 gagctgcggc cattgcctgc cctgcgccga aggccgtccc gggttgtgcg aacccggagc
     2881 ggcggccaat gtcgcgggca cgctgctctc tggcgcacgg cggctcaagc gcggcgcaac
     2941 gccgctcaat caccacatgg gcgtttcggc gttcgcgcaa tacgccaccg tgtcgcgccg
     3001 ctcgctagtg aagattgatc ccgatctgcc gctcgatgaa gcggcgctgt tcggctgcgc
     3061 ggtgttgacc ggggtcggcg cggtgctcaa tacagcgcgc gtcgcgccgg gttcgagcgt
     3121 ggcg
//
LOCUS       DATIOW010000212         2202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1615961, whole genome shotgun sequence.
ACCESSION   DATIOW010000212 DATIOW010000000
VERSION     DATIOW010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615961"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VLL03_07070"
     CDS             <1..330
                     /locus_tag="VLL03_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSS47156.1"
                     /translation="YYPNIMVEWYPHVLVISTVHPRGREACTNVVEFYYPEDIALFER
                     EFVEVEQQAYHETAVEDEEICRRMTQGRKALYLAGENDAGPYQSPMEDGMQHFHEFLQ
                     RQLASHI"
     gene            395..1249
                     /locus_tag="VLL03_07075"
     CDS             395..1249
                     /locus_tag="VLL03_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSS47157.1"
                     /translation="MQSLWMLAAGGLFACMGVFVKLGAAHFSSAELVFYRSLVGLAVI
                     YAIIRHQRLPLATKNWKMHLWRSLSGFASLILYFYAISSLPLATAVTLNYTSPLFLAL
                     LTTLVLKERAHWPLVAAIALGFIGVALLLKPTFNHDQLSIGLIGLTSGFFAGIAYFNV
                     KRLGEIGEPEWRVVFYFSLVCAAGAGAGMLLNTFHGVNGENLFILLGLGTTATLAQLA
                     MTRALGKGNTLVSGSLAYSTVAFASLFGIVLWGEILPLSGWLAIGLIAASGILSIKAA
                     PPRGMPDE"
     gene            1308..1655
                     /locus_tag="VLL03_07080"
     CDS             1308..1655
                     /locus_tag="VLL03_07080"
                     /inference="COORDINATES: protein motif:HMM:NF023390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HSS47158.1"
                     /translation="MISIQPLDNLVTANVIGEFTLADYREFEERVLEKLRSGNKVNVL
                     IDLRDMLHYTLDVAWEEIRFSKNHKHDFGKVAVITKDQWLAWSAWLTGLFVDADIRVF
                     EDDESAQQWLAEK"
     gene            1652..>2202
                     /locus_tag="VLL03_07085"
     CDS             1652..>2202
                     /locus_tag="VLL03_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSS47159.1"
                     /translation="MSKVMFNTLISTAELALHLNDPQWAICDCRHDLANPEAGKRAYA
                     ESHIPGARFVHLEQDLSAPLTGKNGRHPLPDAEKFRATLGRLGIDSSKQVVVYDASGG
                     AFAARLWWMLRWQGHVDVAVLDGGFPKWLAEDRPATSGMPQVHETKFTAVPQKNAWVD
                     AGFVETYVGKPGMLLIDARSPDR"
BASE COUNT          437 a          651 c          633 g          481 t
ORIGIN      
        1 tattacccga acatcatggt cgaatggtat ccgcatgtgc tggtcatcag caccgtccac
       61 ccgcgtggga gggaagcctg caccaatgtg gtcgagttct attaccctga ggacatcgcc
      121 ctgttcgaac gcgaattcgt cgaagtggag caacaggctt accatgaaac cgccgttgaa
      181 gacgaggaaa tctgccggcg catgacgcaa ggccgcaagg cgctctatct cgccggtgaa
      241 aacgatgccg gcccgtacca gtcgccgatg gaagacggga tgcagcactt ccacgagttc
      301 ctgcaacgac agctcgccag ccacatttga tggccacaag gattaaagcc cctccgccct
      361 tcactcttaa ctctcaattc ttaacttcac atagatgcag tccttatgga tgctggccgc
      421 gggcgggctg tttgcctgca tgggcgtgtt cgtcaagctc ggcgcggcac atttttccag
      481 cgccgaattg gttttctacc gctcgctggt ggggcttgcg gtcatctacg ccatcatccg
      541 tcaccagcgg ctgccgctgg caacaaagaa ctggaaaatg catttgtggc gcagcctctc
      601 cggcttcgcc tcgctcattc tctattttta cgccataagc agcctgccac tcgccaccgc
      661 ggtgaccctc aactacacat cgccgctgtt cctcgcgctc ctcaccaccc tcgttttgaa
      721 agagcgcgcc cactggccgc tggtcgcggc aattgccctt ggatttattg gagtggcgct
      781 attgcttaag ccgacgttca atcacgacca gctgtccatc ggcttgatcg gtctgacctc
      841 gggctttttt gccgggatcg cctatttcaa cgtcaagcgg ctgggggaaa tcggtgagcc
      901 cgaatggcgg gtggtgtttt atttcagtct ggtatgcgcc gccggtgcgg gggcagggat
      961 gctgctcaac accttccacg gcgtgaacgg cgaaaacctc ttcatcctcc tcggattggg
     1021 caccactgcg acgctggcgc aactggcgat gacgcgcgcc ctgggcaagg gcaatacgct
     1081 ggtctccggc agcctcgcct acagcacggt ggcgttcgct tcgctgttcg gtattgtgct
     1141 gtggggagaa atcctgccgc tttcaggctg gctggccatc ggcttgattg cagccagcgg
     1201 catccttagc atcaaggccg cgccgccgcg cggcatgccg gacgaatgag gttccttatc
     1261 gcattgcggc ggaaaagatt gttgcctaat ataaaagaga ggccgcgatg atctccatcc
     1321 aacctttgga caacctggtt accgccaacg tgatcggcga atttactctt gccgactacc
     1381 gcgaattcga agagcgcgtg ctggaaaagc ttcgctccgg caacaaggtc aatgttttaa
     1441 tcgacttgcg cgacatgttg cattacaccc tggatgtggc gtgggaggaa atccgcttca
     1501 gcaaaaacca caagcatgat ttcggcaaag tcgcggtgat taccaaagat caatggctgg
     1561 cctggagcgc ctggctgacc ggcctgtttg tcgatgccga catccgggtg tttgaggacg
     1621 acgagtcggc gcaacagtgg ctggcggaga agtgagcaag gtaatgttca acaccctgat
     1681 ttccactgcg gaactcgcac tgcatctcaa tgatccccaa tgggcgattt gcgattgccg
     1741 ccatgacctc gccaatcccg aagcaggcaa gcgcgcttac gccgaatcgc atattcccgg
     1801 cgcacgcttt gtgcacctgg agcaggacct ctccgccccg ctcaccggca aaaacggcag
     1861 gcaccctttg cctgatgcgg aaaaattccg cgccacactt gggcggctgg gcatcgattc
     1921 cagcaagcaa gtagtggttt acgatgcttc gggcggcgcg ttcgccgcgc ggctgtggtg
     1981 gatgctgcgc tggcaggggc acgtcgacgt cgcggtgttg gatggcgggt ttcccaaatg
     2041 gctcgcggaa gaccggccgg ccacttccgg catgccgcaa gttcatgaaa ctaaatttac
     2101 cgccgtcccg caaaagaatg cctgggtgga tgccggcttt gtcgaaacgt atgtgggcaa
     2161 gccgggcatg cttttgattg atgcgcgcag tcctgaccgt tt
//
LOCUS       DATIOW010000213         2965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1108954, whole genome shotgun sequence.
ACCESSION   DATIOW010000213 DATIOW010000000
VERSION     DATIOW010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108954"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VLL03_07090"
     CDS             complement(<1..216)
                     /locus_tag="VLL03_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HSS47160.1"
                     /translation="MIDLYTWSTPNGRKISIMLEEIGLPYRVHPIDLGKGEQFKPEYV
                     AISPYSKIPAIIDEEGPDGTPFKVFESG"
     gene            complement(301..777)
                     /locus_tag="VLL03_07095"
     CDS             complement(301..777)
                     /locus_tag="VLL03_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HSS47161.1"
                     /translation="MFKPALFVLILLVSAFALAEPADKTGEKKHQLEEQFKKADKDND
                     GTLTKEEAKAMPHVAKHFKEIDTDKDGTVSMDEIRAFMKSHIDDAHKRGEERFKKADK
                     DNDGTLTKEEAKAMPNVYKNFDAIDTDKDGTVTLEEIHNFMKAKREEHKATKPQAN"
     gene            complement(835..2052)
                     /locus_tag="VLL03_07100"
     CDS             complement(835..2052)
                     /locus_tag="VLL03_07100"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HSS47162.1"
                     /translation="MKIYAVGGAVRDELLGLKVTDRDYVVVGATPQDMLRLGFKPVGK
                     DFPVFLHPKSHEEYALARTERKTTRGYKGFKIHAAPDVTLEDDLARRDLTINAIAKDE
                     QGKIIDPYGGVKDLEAGILRHVSPAFAEDPVRVLRVARFAARFGFDVAKETLALMREM
                     TQNGEVDALVAERVWQELTKGLMEAKPSRMFEVLRQSGALNKILPELDRLFGVPQPRE
                     QHPEIDTGVHTMLVIDYAASKNFSLPVRFAALTHDLGKGVTPHSQWPRHPGHEAKSVE
                     LVKVLSKRIKVPAACRDLALLAAKYHGETHREPELKPATLVRLLQALDAFRKPQRFAE
                     FLEACGCDFHGRPGFETKPFPQAKYLQGALRAAQSVDAGAIARRLRQPSQIAAKVRAA
                     RIAAVKKWLKQTV"
     gene            complement(2077..2247)
                     /locus_tag="VLL03_07105"
     CDS             complement(2077..2247)
                     /locus_tag="VLL03_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS47163.1"
                     /translation="MTRDNYYSMKIDSVCGCDFPEIFGFKPTALEAAVPLYLAGGTPR
                     GRYSGFRFKARR"
     gene            complement(2248..>2965)
                     /locus_tag="VLL03_07110"
     CDS             complement(2248..>2965)
                     /locus_tag="VLL03_07110"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HSS47164.1"
                     /translation="HLLAAQGRSVRVPTRNRERAKELIVLPTVDVVQADVHDDEELKQ
                     MLTGMDAVINLVGILHEESSGDFRRVHVELPLRIARMCASLGVKRMVHMSALNADANG
                     PSSYLRSKGEAETALKQTAQGLALSVFRPSVIFGRDDTFLNLFAKLLKYFPVLFLARP
                     QARFQPVFVEDVAHAFVLSLDKLDTFGKSYDLCGPKVYTLRELVEYVAQVTGRRRRII
                     GLSDRLSYLQAWAMEFLPLK"
BASE COUNT          573 a          858 c          838 g          696 t
ORIGIN      
        1 acctgattcg aacaccttga atggcgtgcc gtcaggccct tcctcatcaa taatcgctgg
       61 aatcttgcta tacggtgata tcgccacata ctccggcttg aattgttcgc ctttgccaag
      121 atcaatgggg tgcacgcggt atgggagccc gatttcttcc agcatgatgg aaatcttgcg
      181 gccgttgggg gtggaccagg tgtagagatc tatcattctc catccttaag ctcggtgaga
      241 ctcccttttt taaaacaaaa cgccggagat tacaccgccg gcgtagtgat gctcgcccaa
      301 ttaattggcc tggggtttag tggccttgtg ttcctcgcgc ttggctttca tgaagttgtg
      361 gatttcctca agcgtaaccg tgccgtcctt gtcggtgtct atggcatcga agttcttgta
      421 gacattgggc atggccttgg cttcttcctt ggtaagcgtg ccatcgttgt ccttgtcggc
      481 ctttttgaag cgttcctcgc cgcgcttgtg cgcatcgtct atatgggatt tcatgaaggc
      541 gcggatttca tccatggaaa cggtgccgtc cttgtcggta tcgatttcct tgaaatgttt
      601 ggcgacatgc ggcatggctt tggcttcctc tttggtaagc gtgccatcgt tatccttgtc
      661 ggctttcttg aattgttcct ccagctggtg ctttttttct ccagtcttgt ccgcgggctc
      721 ggctagtgca aatgcggaaa ccagaaggat caaaacaaac aaagcaggtt tgaacatggt
      781 gttctccttt aatggaataa gaaatgatat aaacgtttcg aggccggggg tagtttacac
      841 ggtttgcttg agccattttt tgaccgccgc aatccgtgcc gcgcgcactt tggcggcgat
      901 ttgactgggc tggcgaagcc gcctggctat tgcgcccgca tccacgcttt gcgcggcacg
      961 cagtgcgcct tgcaggtatt tggcctgcgg gaaaggtttg gtttcaaacc ccggacggcc
     1021 atggaaatca cagccgcagg cctcaagaaa ttcagcaaag cgctgcggct tgcggaaagc
     1081 gtcaagcgcc tgaagcaggc ggacgagggt ggcaggcttg agttccggtt cgcggtgcgt
     1141 ctcgccatgg tatttggcgg cgagcagcgc cagatcgcgg caagcagcgg gaaccttgat
     1201 gcgcttggac agcactttga cgagctccac gctctttgcc tcatggccgg gatggcgcgg
     1261 ccattggctg tgcggcgtca cccccttgcc gaggtcgtgg gtgagcgccg caaaacgcac
     1321 cggcagtgaa aaattctttg aggcggcata gtcaatcacc agcatggtgt gcacgccggt
     1381 gtcaatttcc ggatgctgct cccgaggttg cggcacaccg aacaagcggt ccagttcagg
     1441 caggatttta ttgagagcgc cgctttggcg caatacttcg aacatgcgcg agggttttgc
     1501 ttccatcaac cctttcgtca gttcctgcca tacccgctct gcgaccagcg cgtccacttc
     1561 gccgttttgc gtcatttcgc gcatcagcgc cagcgtttct ttggcgacgt caaaaccgaa
     1621 gcgcgcagca aaccgcgcca cgcgcagaac gcgcaccggg tcttcggcaa aagccgggct
     1681 gacatggcgc agaattccgg cctcgaggtc cttcacgccg ccgtaagggt caatgatttt
     1741 gccctgctcg tccttggcga tggcgttaat ggtgaggtcg cggcgcgcca aatcatcttc
     1801 cagcgtgaca tcaggggcgg catggatttt gaagcctttg tagccgcggg tggtcttgcg
     1861 ctcggtgcgc gccagtgcgt attcctcgtg gcttttggga tggaggaaca ccgggaaatc
     1921 cttgcccacc ggcttgaacc ccaagcgcag catgtcctgc ggcgtggccc ccaccaccac
     1981 ataatcgcga tccgtcactt tgagacccaa tagctcatcg cgcaccgcgc cgccgactgc
     2041 ataaattttc attggccaat atgggtcaag ccggccttaa cgccgcgcct tgaaacggaa
     2101 cccgctgtag cgtccgcgcg gcgtgccgcc ggcgagatac aacggcaccg ccgcttccag
     2161 cgcagtcggt ttgaagccga aaatttccgg gaaatcgcac ccgcacacgc tgtctatttt
     2221 catcgaatag tagttatcgc gggtcatcta ctttagcggc agaaattcca tagcccacgc
     2281 ctgcaagtac gatagccggt cgctcaagcc gatgatgcgg cgtctgcgcc cggtgacttg
     2341 cgcgacatat tccaccagtt cgcgcagcgt atacactttc ggcccgcaca agtcatagct
     2401 cttgccaaaa gtgtccagct tatcgaggct caacacaaaa gcatgcgcca cgtcttccac
     2461 gaataccggc tgaaaccgcg cctgcggtct ggcgagaaac agcaccggaa aatatttcag
     2521 gagtttggca aacaggttga gaaaagtgtc atcgcggccg aaaatcaccg aaggacggaa
     2581 aacgctcagc gcaagtccct gcgctgtttg cttgagcgcc gtttccgctt cgcccttgga
     2641 gcgaagatag ctgctagggc cgttggcgtc ggcattgagc gcgctcatgt gcaccatgcg
     2701 cttcacccct aggcttgcgc acatcctggc gatgcgcagc ggcagctcga catgcacccg
     2761 cctgaagtct ccgctgctct cctcgtgcag aatgcccacc aggttgatta ccgcgtccat
     2821 gcccgtaagc atttgcttaa gctcttcgtc atcgtggacg tcggcttgca ctacgtctac
     2881 ggtgggcagc acgatgagct ctttggcacg ctcgcggttg cgcgtcggca cgcgcacact
     2941 gcgtccctgc gcggcaagca aatgc
//
LOCUS       DATIOW010000214         4218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_294922, whole genome shotgun sequence.
ACCESSION   DATIOW010000214 DATIOW010000000
VERSION     DATIOW010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294922"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VLL03_07115"
     CDS             <1..439
                     /locus_tag="VLL03_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HSS47165.1"
                     /translation="YGSSPFAETSAKQLGLQPVMTLQSQIIATQDLQPGDKVGYTGVF
                     TAEKRMKVGIVACGYADGYPRHARTGTPVNVNGRITRTLGRISMDTLCVDLSDIPDAA
                     VGTQVALWGDGNPIEDVAKAAGTVSYELFTKLNPRVPVVETHG"
     gene            432..1781
                     /gene="radA"
                     /locus_tag="VLL03_07120"
     CDS             432..1781
                     /gene="radA"
                     /locus_tag="VLL03_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HSS47166.1"
                     /translation="MAKPRSVYVCTECGGQAPKWQGQCPHCSAWNTLVETVVEAVSKN
                     RFSSTAASKLQSLGEVEAQEQPHQPTGISEFDRVLGGGLVQGAVVLLGGDPGIGKSTL
                     LLQALCHMGAQHNVLYESGEESAQQIALRARRLALDAKPLQLLAEIQLEKIIATLRSQ
                     KPEVAVIDSIQTLYAEALQSAPGSVAQVRECAAHLARYAKQSGTAIIFIGHVTKEGTL
                     AGPRVLEHIVDTVLYFEGDTHSSFRLIRAVKNRFGAVNELGVFAMTEKGLKGVNNPSA
                     LFLSQHGQEVAGSCVLVTQEGTRPLLVEIQALVDDAHAPNPRRLTVGLEQNRLAMLLA
                     VLHRHAGIACFDQDVFVNAVGGVKIAEPAADLAVLLAIVSSLKNKPLSQKLVVFGEVG
                     LAGEIRPVQRGQERLKEAAKLGFTQALTPKANKPRQPLDSIEVIAVERVEDAVAKIR"
     gene            1885..3525
                     /locus_tag="VLL03_07125"
     CDS             1885..3525
                     /locus_tag="VLL03_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HSS47167.1"
                     /translation="MTRVNPYNTDLDKNPANYAPLSPLSFLERTAYVYPRRIATIHCD
                     WKITWAETYARCRRLASALVKRGMGVGDTVAVMLPNIPQMFEVHFGVPMSGAVLNTLN
                     TRLDAEAITFMLKHGGAKVLITDREFSATVAKALTMLDKKPLVIDVDDPGFKGGELLG
                     EMDYEAFLKIGDPNFVWSLPEDEWDAISLNYTSGTTGNPKGVVYHHRGAYLNAVSNII
                     DWDMPRHSVYLWTLPMFHCNGWCFPWTVAANAGTNVCLRRVEAKLILAAIRQHKVTHY
                     CGAPIVHSMLINAPTEFKAGINHTVSGLVAAAAPPAAMIEGMERLGFNITHVYGLTET
                     YGPASVCSKHTEWEQLNISERVERNGRQGVRYTMQEGMTVMDPETMQPVPWDGQTMGE
                     IMFRGNITMKGYLKNSKASTEVFKGGWFHSGDLAVMQPDGYVKIKDRSKDIIISGGEN
                     ISSLEVEDVLYRHPAVMAAAVVAQPDPKWGEIPCAFVELKEGAEVTEQEIIEHCRSYM
                     ARFKVPRKVIFGVLPKTSTGKIQKFILRERARSASAIE"
     gene            3675..>4218
                     /locus_tag="VLL03_07130"
     CDS             3675..>4218
                     /locus_tag="VLL03_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSS47168.1"
                     /translation="MSSEYILETHNLTKEFKGFVAVNDIGLKIKRGSIHALIGPNGAG
                     KTTVFNLLTRFLAPTNGRIVFNGTDITREKPAQIARHGIIRSFQISAVFPHLTVLENV
                     RIALQHKLGTSFHFWRSEKTLDALNDRAMALLESVDLGAFASTVTVELPYGRKRALEI
                     ATTMAMEPEMMLLDEPTQGMG"
BASE COUNT          933 a         1133 c         1259 g          893 t
ORIGIN      
        1 ctatggttct tcgccttttg ccgagacaag cgcaaagcaa cttggcttgc agccggtgat
       61 gactctgcag agtcagatca ttgcgacaca ggacttacag ccgggtgaca aagtgggtta
      121 caccggcgtt ttcactgccg aaaagcggat gaaagttggc atcgtcgcct gcggctacgc
      181 cgacggctat ccacgccatg cgcgcaccgg cacgccggtg aacgtcaacg gcagaattac
      241 ccgcaccctc gggcggattt cgatggatac tttatgtgtg gatttgagcg atattcccga
      301 cgccgcggtc ggtacgcaag tggcgctctg gggcgatggc aatccgattg aagatgtggc
      361 gaaagcggcg ggaacggtga gctatgaatt gtttaccaag ctgaatccgc gcgtgcctgt
      421 ggtcgaaact catggctaaa cccagatcag tatatgtttg caccgagtgc ggtgggcaag
      481 caccgaaatg gcaggggcag tgcccgcatt gctcggcatg gaataccttg gtcgaaacgg
      541 tggttgaagc tgtttccaaa aaccgcttca gttccaccgc agcgagcaag ctgcaatcct
      601 tgggtgaagt ggaagcgcag gagcagccgc accagcccac cgggatatcc gagtttgacc
      661 gcgtgctggg cggcgggctg gtgcagggcg cggtggtgtt gttgggcggc gacccgggta
      721 tcggcaaatc cactctgctg ctgcaggcgc tgtgtcatat gggcgcgcag cacaacgtgc
      781 tttacgagag cggcgaagaa tcggcgcagc aaatcgcgct gcgcgcacgg cgccttgcgc
      841 tcgatgccaa accgttgcaa ttgctcgcgg aaattcaact ggaaaaaatc atcgccacgt
      901 tacgctcgca aaagccggaa gtggcggtga tagattcaat tcagaccttg tatgccgaag
      961 cgctgcaatc ggcgccgggc tcggtggcgc aggtgcgcga atgcgcggcg catctcgcgc
     1021 gctacgccaa gcaaagcggc acggcgatta tttttatcgg gcacgtgacc aaggagggaa
     1081 cattggccgg cccgcgcgtg ctggagcaca ttgtggacac cgtgctgtat ttcgaaggcg
     1141 acacgcattc cagcttccgc ttgatccgcg cggtgaaaaa ccgttttggc gcggtcaatg
     1201 agctcggtgt gttcgccatg accgagaaag gcttgaaagg cgtcaacaat ccttcagcgc
     1261 tgtttctctc acaacatggc caggaggttg cagggtcctg cgtgctggtg acgcaggaag
     1321 gcacgcggcc gctgctggtg gaaatccagg cgctggtgga tgacgcgcac gcgccgaatc
     1381 cgcggcgcct cacggtgggg cttgagcaaa accgtcttgc gatgctgctc gcggtgctgc
     1441 accgtcacgc cggcatcgcg tgtttcgatc aggatgtgtt cgtcaacgcc gtgggtggag
     1501 tgaaaatcgc cgagcccgcc gccgatttgg cggtgttgct agcgatcgtc tcctcgctca
     1561 agaacaagcc gctgtcacaa aagctggtgg tgttcggcga agtgggtttg gccggcgaaa
     1621 tccgcccggt gcagcgcggt caggagcgtc tcaaggaagc ggccaagctg ggcttcacac
     1681 aggcgctgac tcccaaagcc aacaagccca ggcagccgct ggacagtatt gaagtaatcg
     1741 ctgtggagcg ggtggaagac gcggttgcca aaatccgcta accggcaacc gttgctattc
     1801 gcggtgcaag caggtaaatt catcttgagc aagtcaattg gtaaggcact tagttttttc
     1861 gctatttaag ttaaagaggg cagcatgact cgagtcaatc cttacaacac tgacttggac
     1921 aaaaatcccg cgaattacgc gcccctttct ccgctgagtt tcctggagcg cactgcttat
     1981 gtctatccca ggcgcatcgc caccatccac tgtgactgga aaatcacctg ggctgaaact
     2041 tacgcgcgtt gccggcgtct ggcttccgct ctggtaaagc gtggcatggg tgtcggcgat
     2101 acggtggcgg tgatgttgcc taatattcct cagatgttcg aagtgcattt cggtgtgccg
     2161 atgtcaggcg cggtgctgaa caccttgaat acccggctcg atgcggaagc gattaccttc
     2221 atgctcaagc acggcggggc caaagtgctg attaccgacc gcgagttttc cgcgaccgtc
     2281 gccaaggcgc tcaccatgct ggacaaaaaa ccgctggtga ttgatgttga cgatcccggg
     2341 tttaaaggtg gcgagctttt gggcgagatg gattacgaag cattccttaa aatcggcgat
     2401 ccgaatttcg tctggtcgct tcccgaagat gagtgggatg ctatttcgct taactacact
     2461 tccggcacta ccggcaatcc caagggcgtg gtgtaccatc atcgcggtgc gtacctcaac
     2521 gcggtgagca acatcataga ttgggatatg ccgcgccatt cggtttatct atggacgctg
     2581 ccgatgtttc actgcaatgg ctggtgtttt ccctggaccg tggcggcgaa tgccggcact
     2641 aatgtgtgcc tgcgccgcgt ggaagccaag ctgatactgg ctgcaatccg ccagcacaaa
     2701 gtgacgcatt attgcggcgc gccgatcgta cacagcatgc tgatcaatgc ccctaccgag
     2761 tttaaagccg gcatcaacca caccgtcagc ggcctggttg cggcagcggc cccgcctgcg
     2821 gcgatgattg agggcatgga gcgcctgggc ttcaacatca ctcatgtgta tgggctcact
     2881 gaaacctacg gaccggcctc ggtatgctcc aagcatacgg agtgggagca gctcaatatt
     2941 tccgagcgtg ttgagcgcaa cggcaggcag ggtgtgcgtt ataccatgca ggaaggtatg
     3001 acggtgatgg atccagaaac catgcagccg gtgccgtggg acgggcagac catgggcgag
     3061 atcatgtttc gaggcaacat taccatgaaa ggctatctca agaactcgaa ggcaagcacg
     3121 gaagtgttta aggggggctg gttccattcc ggcgatctgg cagtgatgca gccagacggt
     3181 tacgtgaaaa taaaggatcg ctccaaagat ataatcattt ccggaggtga aaatatctcc
     3241 tcgctggaag tggaagacgt gctctatcgc caccctgcgg tgatggcggc agcggtagtg
     3301 gcgcagcctg atccaaagtg gggcgagatt ccctgtgcgt tcgtcgaatt aaaagagggc
     3361 gccgaagtga ccgagcaaga aatcatcgag cattgccgca gctatatggc gcgcttcaag
     3421 gtgccgagga aggtgatatt cggcgtgctt cccaagactt cgacgggtaa aatacagaaa
     3481 ttcatcctgc gcgagcgcgc caggtcggct tcagcgattg aataagcatt gcccactgtg
     3541 tgacattacc ccacccgtac tagcgctggt tggcatactt ctctatactt tctataaagt
     3601 aaaaacactt cctcggtttt ttctgaataa accggccacc ggttcacact gaccgtaaaa
     3661 acagcggatt ggcgatgagc agcgaataca tactggaaac gcacaatctc accaaggaat
     3721 tcaaaggttt tgtggcggtt aacgatattg gtctgaaaat caagcgcggc agcattcacg
     3781 ccttgattgg acccaacggc gccggcaaga ccacggtctt caatttgctc acccgatttt
     3841 tggctcccac caacggccgc atcgttttca acggtaccga tatcacccgg gaaaaaccgg
     3901 cgcaaattgc gcggcacggc atcatccgct cattccagat ttccgcggtg tttccgcatc
     3961 tcaccgttct ggaaaacgtg cgcattgcgc tacagcacaa gcttggcact tcattccatt
     4021 tctggcgctc ggagaaaacg ttggatgcgc tgaatgaccg cgcgatggca ttgctggaat
     4081 cggtcgacct cggtgcgttt gcttcgacgg tcaccgtcga acttccttac ggcagaaagc
     4141 gtgcgctgga aattgccacc acgatggcca tggaacctga aatgatgctg ttggatgagc
     4201 cgacccaggg catgggcc
//
LOCUS       DATIOW010000215        10588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1514489, whole genome shotgun sequence.
ACCESSION   DATIOW010000215 DATIOW010000000
VERSION     DATIOW010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10588
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514489"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2159)
                     /locus_tag="VLL03_07135"
     CDS             complement(<1..2159)
                     /locus_tag="VLL03_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HSS47169.1"
                     /translation="MSTASEFPFLPRASLIQTAEQGATLITPNRRLAAYLKREFDAGQ
                     IKRGRSVWPAADILPYSAFLERAWSELARIERRGATLLSTEQEIALWERVIADSPQGD
                     ILLNSSAAARQAREAWSIRHAFRIDFARYAAMLDEDSAVFRGWAQRYGKICAEKNWLD
                     GARLADVIAAAGAALKPRQIVLYGFDELSLQQQTFFEALAAAGGSVSEAKPEANASPA
                     VRAGFPDAEAELYAVASQIKQTLVERPDANIGVIVPDLSARRADLMRIFDDVLQPARV
                     VSASRERARPYNVSLGLALTDYPLVASAFLILDLARGELALDDMGSLLRSPFLAAAEQ
                     EFTVRALLDARLRQSGRLSVTLKMLARETHGAPQLAARLNTWIERASSARKTKQPPSA
                     WSAVFQQLLAGLGWPGERTLDSEEYQTFGKWREIVSQLSALDLIAPRLGYDEALKNLK
                     RLAGDSTFQPESPEVPVQVLGVLEANALEFDLLFVTGLTDEAWPEPPHPNPFLPLAVQ
                     RALSVPHAGADWQLEFARRATHNWRACAKEVHFSYPQRDGDRELRPSPLLREVPEAQP
                     ALENAALYRRVIFAERRVETLADFKAPRLPAGIEVRGGADFFKNQAACPFRAFALHRL
                     GAESVEAGHAGLDARDRGLLMHRAAENLWKQLKNSKQLAAASEEELKAAAGRAAKNAL
                     ESMRKKRPDVMTQAFTALEQERLASLLMRLLELEKNRA"
     gene            complement(2268..2591)
                     /gene="fdxA"
                     /locus_tag="VLL03_07140"
     CDS             complement(2268..2591)
                     /gene="fdxA"
                     /locus_tag="VLL03_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HSS47170.1"
                     /translation="MTYVVTESCIKCKYTDCVDVCPVDCFREGPNFLVIDPAECIDCT
                     LCVAECPVEAIFAEDDVPENQKHFTALNAELSKNWKPIIEKKAAPPDADEWTKVKDKL
                     KLLER"
     gene            complement(2914..3225)
                     /locus_tag="VLL03_07145"
     CDS             complement(2914..3225)
                     /locus_tag="VLL03_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS47171.1"
                     /translation="MLTISYASEKPSVWEAALVLVKILFRVTLLLAASELAKRSTFLG
                     AIVIALPPTFMLAMTWLYLDTRDADKVAVFARDIFFLVQLRCCFPAFPAAAGEPLAVL
                     V"
     gene            3273..3599
                     /gene="trxA"
                     /locus_tag="VLL03_07150"
     CDS             3273..3599
                     /gene="trxA"
                     /locus_tag="VLL03_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HSS47172.1"
                     /translation="MGSQIHHVTDDNFEAEVLQAPVPVLVDYWAEWCGPCKIIAPVLD
                     EVAQEYAGRLKIAKLNIDENQATPPKYGIRGIPTLMLFKNGNIEATKVGALSKSQLTA
                     FIDSNI"
     gene            3748..5007
                     /gene="rho"
                     /locus_tag="VLL03_07155"
     CDS             3748..5007
                     /gene="rho"
                     /locus_tag="VLL03_07155"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HSS47173.1"
                     /translation="MHLSDLKTRHVTELVDMAITNEIDGANRLRKQELIFALLKNQAR
                     KGESIFGEGTLEVLPDGFGFLRSPDTSYLAGPDDIYVSPSQIRRFNLHTGDSIEGEIR
                     TPKDGERYFALVKVDKVNSEPPENSKHKILFENLTPLFPTEHFVLERDIKAEENITGR
                     VIDMIAPIGKGQRGLLVSPPKAGKTVMLQHIAHSIAANFPEVVLIVLLIDERPEEVTE
                     MQRSVKGEVVSSTFDEPATRHVQVAEMVLEKAKRLIEHKKDVVILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDDVIYEE
                     FKGTGNMEIHLDRRMHEKRIYPAINVNRSGTRKEELLIKPEVLQKIWVLRKLLYPMDD
                     LEATEFLLEKLRGTKNNADFFDSMKRG"
     gene            5403..5618
                     /gene="rpmE"
                     /locus_tag="VLL03_07160"
     CDS             5403..5618
                     /gene="rpmE"
                     /locus_tag="VLL03_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HSS47174.1"
                     /translation="MKPDIHPKYDEIAVTCSCGNKFKTRSTLGRALHIEVCSVCHPFY
                     TGKQKIVDTAGRVEKFRQKYSRKKTAS"
     gene            complement(5844..6287)
                     /gene="cynS"
                     /locus_tag="VLL03_07165"
     CDS             complement(5844..6287)
                     /gene="cynS"
                     /locus_tag="VLL03_07165"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HSS47175.1"
                     /translation="MTRHEVTEKIISAKLKKGLKWADIAKKVGQSKEWTTAALLGQMT
                     LTGEQAQTVGKLLGLSEEEIALLQVVPYKGSLGETVPTDPLIYRFYELIQVYGTTIKE
                     LIHEEFGDGIMSAIDFSMDIERQPDPKGDRVKIVMSGKFLPYKIY"
     gene            6308..6475
                     /locus_tag="VLL03_07170"
     CDS             6308..6475
                     /locus_tag="VLL03_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS47176.1"
                     /translation="MFIKNNNGRQNIVHHFNNFCLQQAWVAQPQNFPSDYAVINLKPF
                     ILHEAQAKITA"
     gene            6472..7926
                     /locus_tag="VLL03_07175"
     CDS             6472..7926
                     /locus_tag="VLL03_07175"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HSS47177.1"
                     /translation="MKQLHVLMFGAVLPALLAVGCATNPVTGNPNFVVMTEAQEISTG
                     RSADAEVRKQYYVYDVQELQDYVNQVGQKLAHNSHRPNLSYHFTVLDSTEVNAFALPG
                     GYIYITRGIVSYLNSEAELGAVLGHETGHVTARHSVQQISAATAANVGVQLASVFLPG
                     LNSMGGSTLTNLLGNVLLSGYGREHELEADRLGAEYLGRAGYDPQAMVKVIGVLKNQE
                     LFDAEVAKQEGREPRAYHGLFATHPDNDTRLQQVVTEAQSVTRPDAVVNRDAFLKHID
                     GMIFGDSPQQGIVRNGSFYHGELGLALKFPEGWRIHNLPDRVQAQSPGNDAGVELRLG
                     ENKPEGTPADYLRKLLRLTFGVEIQSLTLNGFPAATAVLSSQGKPVRVTVIYFNNHAF
                     LIVGSAASKSAFERYQENFNTTASSFHAITDAERRLAKPLKIKIINAREGLTYAELAK
                     NSPLGKNAENHLRLFNAQYPSGEPLRGQALKIVE"
     gene            7957..8847
                     /locus_tag="VLL03_07180"
     CDS             7957..8847
                     /locus_tag="VLL03_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo hydroxylase family protein"
                     /protein_id="HSS47178.1"
                     /translation="MLAQSEIIEVLDNREWRFTLAGDAAAQTARAVEAGKVLFFPKLA
                     FTLEPNERCFLSPACSDGKAKNISYDCRNHMLQGTGLAGKSRDELSAMIGRFARKART
                     LIESLLPGYSARLEQALASYRPMQVESRTLSRRKDDSRLHVDAFASRPNQGKRILRVF
                     TNINPDGEPRVWNIGEPFECFAPKYVPQISLPLPGSAWFLERLYITKGRRSPYDHVML
                     QLHDRAKLDDRYQQNADKLTFNFTPGSTWVMFSDRVLHAALSGQYLLEQTFYLPVAAM
                     QDDGRSPLRILERLYHRELA"
     gene            8847..10529
                     /locus_tag="VLL03_07185"
     CDS             8847..10529
                     /locus_tag="VLL03_07185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSS47179.1"
                     /translation="MMYFPDNPFQTEHAPGIKLSHITATCLLCLAWILPGLIGHEPWK
                     PDEAHSFGLVFHILNHGDWVVPTLAGEPYMEKPPFFYLSAAFFGWLFSPFLPLHDAAR
                     LASGFYIAVTLVCIGLAGRALYGRGQGTMSALLLLGCLGLLVRAHQLVSDIGLLAGFA
                     LCLYGLALNREHPLQAGLLTGTGVGLGFMSRGTLAPMVMALTCLLLPLLFREWRDRRY
                     ALTILIAAIASAPWLIIWPYALYQHSPELYHQWLWVQDLERLIGLAPAGNEASAWYFL
                     KTLPWFAWPALPLALWTLWHGRRKIFNRAEIQLPLVLLVALYAVLSLDTDTHDLDALP
                     LLLPIVLLATPALGTLTRSAANALDWFGIMTFGLLALGLWLGWLALMTGSPAGIATRL
                     NEYLPGYNLPFNLFHFLIAVAFTLGWLSLISRIGRSNRRAVINWAGGITMVWMLAMTL
                     WLPWLDVSKNYRWMMASLQQSLPAEFQCISSRGLGKTQRSMLEYYTGIITRRMELSGT
                     ADCDLLLIQSRSQETPSVSPGWEKIWEGSRPGDLNERYRLYQKQERSYATQG"
BASE COUNT         2246 a         3209 c         2982 g         2151 t
ORIGIN      
        1 gcgcgatttt tttccagctc gagcaagcgc atcaagagcg acgcgaggcg ttcctgctca
       61 agcgcggtga aagcctgagt catgacatcg gggcgtttct tgcgcatgct ctccagcgca
      121 tttttcgccg cgcggccagc cgccgctttc aattcttcct cgctggcggc ggcaagctgc
      181 ttgctgtttt tgagttgctt ccaaagattt tccgcagcgc ggtgcatgag caaaccgcgg
      241 tcgcgggcat ccaatcccgc atgacccgct tccacgctct ccgcgccaag gcggtgcaac
      301 gcgaaagcgc ggaacgggca cgccgcctgg ttcttgaaga aatccgcgcc gccgcggact
      361 tcgataccgg cggggagccg cggcgctttg aaatcagcga gcgtctcgac cctgcgttca
      421 gcaaagatga cgcggcgata gagcgcggcg ttttccagcg ccggctgcgc ttcaggcact
      481 tcgcgcaaca aagggctggg cctcaattcc cggtcgccgt cgcgctgcgg gtaactaaag
      541 tggacttctt tcgcgcaggc gcgccagtta tgggtcgcgc ggcgcgcgaa ttcgagctgc
      601 cagtcggcgc cggcatgcgg cacgctgagc gcacgctgca ccgcgagcgg caggaaggga
      661 ttggggtgag gcggctccgg ccacgcttcg tcggtcaagc cggtgacaaa caagagatca
      721 aactccaggg cgtttgcttc cagcacgccc agcacctgca ccggcacttc cggtgattcg
      781 ggctgaaacg ttgaatcgcc ggccagccgc ttcaggttct taagcgcttc gtcataacca
      841 aggcgcggcg cgatgagatc gagcgcggag agctgcgaca cgatttcgcg ccacttgccg
      901 aaagtttgat attcctcgga atccagcgtg cgctcgcccg gccagcccag gcccgcgagc
      961 agttgctgga agacggcgct ccacgcagaa ggcggctgtt tcgttttcct cgcgctgctc
     1021 gcccgctcta tccatgtatt aagacgcgca gcgagttgcg gggcgccgtg cgtttcacgc
     1081 gcgagcattt tgagcgtgac gctgagccgc ccgctttggc gcaggcgcgc atcgagcagt
     1141 gcgcgaacgg tgaactcctg ttccgccgcc gcgaggaaag gacttctcag cagcgaaccc
     1201 atgtcatcca gcgccaactc gccgcgcgct agatcgagaa taaggaatgc gctcgccacc
     1261 agcggataat cagtgagcgc caatcccaat gaaacattgt agggccgtgc gcgctcgcgc
     1321 gaggcggaca ccacgcgcgc cggctgcagc acgtcgtcga agatgcgcat gaggtccgcg
     1381 cggcgcgccg agagatcggg aacaataacg ccgatattcg catccggccg ctccacaagc
     1441 gtctgcttga tttgcgacgc gacggcgtac agctcggcct cggcatcggg gaaacctgcg
     1501 cggacggcag gcgaagcgtt cgcttccggt ttcgcttcgc ttacgctgcc gcccgccgca
     1561 gccagcgctt cgaaaaacgt ctgctgctgg agcgaaagct catcaaaacc atagagcacg
     1621 atttgacgcg gtttgagagc cgcaccggcg gcagcgataa catcggccag ccgcgcgccg
     1681 tcaagccagt ttttttctgc acagattttt ccatagcgct gcgcccaacc gcggaagacc
     1741 gcgctgtcct catcaagcat ggcggcgtag cgcgcgaaat caatgcggaa agcatggcgg
     1801 atggaccaag cctcgcgcgc ctggcgcgcg gcggctgagg aattgagcag gatatctcct
     1861 tgcggcgagt cggcaatcac ccgctcccac agcgcgattt cctgttctgt ggaaagcagt
     1921 gttgcaccgc ggcgctcaat gcgcgcaagc tccgaccatg cacgttccag gaacgcgctg
     1981 taagggagga tatcggccgc aggccagacc gaacggccgc gcttgatctg cccggcgtcg
     2041 aattcgcgct tcaagtatgc cgcgagccgg cggttgggag tgatgagagt ggcgccttgc
     2101 tcagcggtct ggatgagtga ggcgcggggc aggaagggaa attccgaggc tgtgctcatt
     2161 ggctgtcgcg gcacatattg ttctggaagc gccgcgacat ttcggcacgt gcagcccgtg
     2221 tcggttaact gcgggcggtc cgggatgaaa cttcttaaac ggtcggctta acgctccagg
     2281 agcttcaact tatccttgac cttggtccac tcgtcggcgt cgggaggggc ggctttcttc
     2341 tcaatgatgg gtttccaatt cttggaaagc tcggcattga gcgcggtgaa atgcttctgg
     2401 ttctccggca cgtcatcctc ggcaaaaatc gcttccaccg ggcattccgc gacgcacagg
     2461 gtgcagtcaa tgcattcggc ggggtcgatc accagaaaat tcggtccttc gcggaagcag
     2521 tccaccgggc agacgtccac acagtcggtg tacttgcatt taatgcagct ctcggttaca
     2581 acgtaagtca tttcaaccgt ccttttgcgg caaacggctc taaagtaatt attttaccag
     2641 aaatcgataa gccggcgcgc cccggactcg gactcgcgca gggcgcgcgg acacgttact
     2701 ggcaaagcca gctgtttgcg ggaagcgcgt cattggcccg cccccactcc ggatggctcc
     2761 gccaacgccg tgtcggcggg cctggagcgg gaaggggcgc aagctgccgc aataactacc
     2821 gttaaacggg gcgctcatgc tgccgctccc ggcaccccaa gcttcgctca ccgtgttgcg
     2881 cccccggcca tcagcgcaag gcccatgccg aaattaaacc agaacggcca gtggctctcc
     2941 ggctgcagca ggaaaggcag gaaaacaaca gcgaagttgc accaggaaga atatgtccct
     3001 ggcaaacacc gccaccttgt ccgcatcgcg agtgtcaaga tacagccaag tcatggccag
     3061 catgaaagtg ggcggcagag caatcacaat cgcgccgaga aaagtcgagc gcttggccag
     3121 ctcggaggcc gccagcaaca gtgtgactct aaacaggatt ttgacgagaa ctaaggcggc
     3181 ttcccaaacc gaaggttttt cgctagcata tgatatcgta agcatcgttt caccaccctc
     3241 ctgtagccta tttcccctaa cgcgaggatt tcatgggtag tcaaatacat cacgtcaccg
     3301 acgacaattt cgaagccgag gtgctgcagg cccccgtgcc ggtactggtg gattactggg
     3361 cggaatggtg cggtccatgc aagataattg cgccggtact cgatgaagtg gcgcaggaat
     3421 acgcagggcg cctcaagatt gccaagctca acatcgacga gaaccaggcg acacccccga
     3481 aatacggcat ccgcggcatc cctaccctga tgttgttcaa gaacggcaac atcgaagcca
     3541 ccaaagtagg cgcgctgtcg aaatcgcagt tgaccgcctt tattgacagc aatatttaaa
     3601 ctcgttactc ttgtcgaaca taacttcttg aagtccgcta taataagtac tggtagtgca
     3661 gcaagcagct gcgggcggag gtcccgttcc gcatcccctt aatttcccgg ttctctcgtt
     3721 ttcactctca ccgagcattc tccacttatg catttatccg acctgaaaac ccgccatgtc
     3781 accgaactcg tcgacatggc catcactaat gaaatcgacg gcgccaaccg cctgcgcaag
     3841 caggagctga ttttcgccct gctcaagaac caggcgcgca aaggcgaaag catctttggc
     3901 gaaggcacgc tggaagtgct gcccgacggc ttcggcttcc tgcgctcgcc cgatacgtct
     3961 tatctcgccg ggcccgacga catttacgtg agcccctcgc agatccggcg ctttaacctc
     4021 cacaccgggg attcgataga aggggaaatc cgcacgccca aggacggcga gcgctatttc
     4081 gcgctggtca aggtggacaa ggtcaattcc gagccgccgg aaaattccaa gcacaaaatc
     4141 ctgtttgaaa acctgacgcc gctcttcccc accgaacatt tcgtcttgga gcgcgacatc
     4201 aaggccgagg aaaacatcac gggacgggtc atcgacatga ttgcgccgat cggcaagggg
     4261 cagcgcgggc ttctcgtatc gccgcccaag gccggcaaga cggtgatgct ccagcacatc
     4321 gcgcattcga tcgctgcgaa ttttcccgaa gtcgtgctga tcgtgctgct gatagacgag
     4381 cggccggagg aagtgacgga aatgcaaaga tcggtcaagg gcgaagtggt gtcctccact
     4441 ttcgatgagc cggccacccg ccacgtgcaa gtagcggaga tggtgctgga aaaggcgaaa
     4501 cgcctgattg agcacaaaaa agacgtggtg atcctgctcg attcgatcac gcgcctcgcc
     4561 cgcgcctaca acaccgtggt gccggcttcc ggcaaggtgc tgaccggcgg tgtggatgcg
     4621 aacgccctgc agcgccccaa gcgcttcttc ggcgccgcgc gcaacatcga ggaaggcggc
     4681 tcgcttacta taatcgccac cgcgttgatt gataccggct cgcgaatgga tgatgttatt
     4741 tatgaagaat tcaaaggcac cggcaacatg gaaatccatc tcgaccggcg catgcacgag
     4801 aaacgcatct atccggcgat caacgtcaac cgctccggca cccgtaagga agagttgctc
     4861 atcaagcccg aggtgctgca gaaaatctgg gtactgcgca agctcctcta cccgatggac
     4921 gatctggaag ccactgaatt cctgttggag aagctgcgag ggaccaagaa taacgccgat
     4981 ttcttcgact caatgaaaag aggctgatct taaatttacc gaagttccat ggtaaattcg
     5041 catttacctg aagtccgctg ccgctgctcc cgcgccccag gcttcgccga ccgtgtcgca
     5101 acacgctccg gatcacggag agcgcagctt tgagcggaga cggagcgaag ccgctgcagg
     5161 aaacactgtg atcggagcta atgccgctgc tcccgcactc caggcttcgc cgaccgtgtc
     5221 gcagcggctg cttcttcgat ttaatgaaga ggggctagca cgtaactcct tgctggactt
     5281 cgaataagca cctataaaaa agtcggctgc cccgatccgg cttgcaacag catgtgattt
     5341 gacgcataat tgccgccttt cgcaaaaccg ggaaccgcgg aggttcctcc agagacatca
     5401 ccatgaaacc ggacatccat ccgaaatacg acgaaatcgc cgtaacctgc agttgcggca
     5461 acaagttcaa gacccgctcc acgctgggcc gggcgctgca catcgaggta tgctcggtgt
     5521 gccatccgtt ctacaccggc aagcagaaaa tcgtggacac cgccggtcgc gtcgaaaaat
     5581 tccgccaaaa atacagccgc aaaaaaactg cgtcttaatt gtcacgcatg ttaaattaaa
     5641 ggtggctgcg gctgccttta attttttgaa gagaggactc tcatagcttt ctcaggtgcg
     5701 ccggctacga cgacaaattc atctccgagg tggcgcgggt catggaatac agcaaggctt
     5761 acgaaattta taaaagcgac atggagatga taaccgcaag cgaataataa ggggcaacac
     5821 caccttatta ttcgagcagg atttcagtaa attttgtaag gcaaaaactt gccgctcatc
     5881 acgattttca cccggtcacc cttggggtcg ggctgacgct cgatgtccat gctgaagtca
     5941 atcgcgctca tgatgccgtc gccgaactcc tcatgaatca gttccttgat ggtagtgcca
     6001 tagacttgaa tcaactcgta aaagcggtag atcaacggat cggtggggac ggtttcaccc
     6061 aatgaacctt tgtacggaac cacctggagc agcgcaattt cctcttcact caatcccagc
     6121 agcttgccca cagtttgcgc ctgctcgccg gtgagcgtca tttgccccag cagcgcggcg
     6181 gttgtccatt ccttgctctg gccgactttc ttggcgatat ccgcccactt caatcctttt
     6241 ttgagtttgg ctgaaataat tttctcggtt acttcgtggc gtgtcatcat accctcccgc
     6301 attaaaaatg ttcatcaaaa acaataatgg ccgccaaaac attgtccacc atttcaacaa
     6361 tttttgccta caacaagcct gggtggctca gccgcaaaac ttcccgagcg attacgcggt
     6421 catcaacctt aagcccttta tcctgcatga ggcgcaagct aaaataaccg catgaaacaa
     6481 ctgcacgtat tgatgttcgg cgccgttttg ccggcgctgc tggcagtagg ctgcgcgacc
     6541 aacccggtta ccggcaaccc gaatttcgtg gtgatgacgg aagcgcagga aatcagcacc
     6601 ggccgcagcg ccgatgccga ggtcagaaag cagtactatg tttatgatgt gcaggagcta
     6661 caggattacg tcaatcaagt cggccagaag cttgcgcaca acagccaccg gcccaatctg
     6721 agctaccact tcaccgttct tgattccacc gaagtcaacg ccttcgccct gcccggcggc
     6781 tacatttata ttacccgcgg catcgtgtct taccttaatt ccgaagcgga actgggcgcc
     6841 gtgctcggcc acgaaaccgg ccacgtcacc gcgcgccaca gcgtgcagca aatcagcgca
     6901 gcgaccgccg ccaatgtcgg cgtgcaactc gcctcggttt ttctaccggg gctgaacagc
     6961 atgggaggca gcaccctcac caatttgctc ggcaacgtcc tgctctccgg ctacgggcgc
     7021 gagcatgaac tggaggcgga ccgcctgggc gccgaatacc tgggtcgcgc tggttacgat
     7081 ccgcaagcga tggtgaaagt gatcggcgtg ctgaaaaacc aggaattatt cgacgccgag
     7141 gttgccaagc aggaaggccg cgaaccgcgc gcttaccacg ggctgtttgc cacgcatccg
     7201 gacaacgaca cgcgcctgca gcaggtggtg accgaggccc aaagcgtaac caggcctgat
     7261 gccgtggtaa accgcgacgc gtttctcaag cacattgacg gcatgatctt cggcgacagt
     7321 ccgcaacaag gcatcgtccg caacggcagt ttttaccacg gcgaactggg actggcgttg
     7381 aaatttccgg aaggctggcg cattcataac ctgcccgacc gagtgcaggc gcaaagccca
     7441 ggcaatgatg cgggcgtcga gttgcgcctc ggcgaaaaca aaccggaagg cacgcccgcc
     7501 gattacctgc gcaaactact gcggctcacc ttcggcgtgg aaatccagtc actcaccctc
     7561 aacggtttcc ccgccgctac ggcggtgctt tccagccagg gcaagccggt cagggtcacg
     7621 gtcatttatt tcaataatca cgccttcctg attgtcggca gcgcggcttc caaatccgcc
     7681 ttcgagcgtt atcaggaaaa cttcaacacc accgcctcaa gctttcacgc catcaccgat
     7741 gccgagcgca ggctcgccaa gccgctcaaa ataaaaatca tcaacgccag ggagggcctc
     7801 acttatgcgg agcttgcaaa aaattcgccg cttggaaaaa acgccgaaaa ccatctgcgc
     7861 ctgtttaatg cacaataccc ttccggggaa ccgctgcgcg gccaggcgct gaaaatcgta
     7921 gaatgacggc gctacgcgct cacaccaagg aatgacatgc tggcgcaaag cgagattatt
     7981 gaagtgctgg acaaccgcga atggcgcttt acgctcgcgg gcgatgccgc ggcgcaaacc
     8041 gcccgggcgg ttgaagcggg caaagtgctg tttttcccca agctggcttt tactcttgaa
     8101 ccaaacgaac ggtgctttct ttcgcccgct tgctcggacg gcaaagccaa gaatatcagc
     8161 tacgattgcc gcaaccacat gctgcaaggc acgggcctgg caggcaaaag ccgtgatgaa
     8221 ctttccgcga tgattgggcg ctttgcccgg aaagcacgca cgctcattga atccctgctg
     8281 ccgggttatt cggcgcgtct cgagcaagcg ctcgccagtt accggccgat gcaggtggag
     8341 agccgcacgc tttcccggcg caaggacgat tcgcgcctgc atgtagacgc ttttgcttcg
     8401 cgtcccaacc agggcaaaag aattctacgg gtgttcacca acatcaatcc cgacggcgaa
     8461 ccccgggtat ggaacatcgg cgaaccgttc gagtgtttcg cgccaaagta tgtgccacag
     8521 atttcactac ccctgcccgg ctccgcctgg ttcctggagc gtctttacat taccaagggc
     8581 cggcgcagcc cctacgatca cgtcatgctg caactgcacg accgggcgaa actcgacgat
     8641 cgctaccagc aaaacgcgga caaactcacg ttcaacttca cgccgggctc gacctgggtg
     8701 atgttcagcg accgggtgct gcacgcggcg ctctccggcc agtacctgct ggagcaaact
     8761 ttttatctgc cggtcgccgc gatgcaggat gacggccgct caccgctgcg catcctcgag
     8821 cgcctctacc accgggaatt ggcctgatga tgtattttcc cgacaatcct ttccagacgg
     8881 aacacgctcc cggaatcaag ctctcgcata tcaccgccac ctgcctgctt tgcctggcct
     8941 ggattcttcc cggattgatc ggccatgagc cgtggaagcc cgatgaggcg cacagctttg
     9001 ggctggtgtt tcacatcctt aatcacggcg attgggtggt gcccacattg gccggcgagc
     9061 cttacatgga aaaaccgccg ttcttttatt tgagcgccgc tttcttcggc tggctcttct
     9121 ccccgttttt gccgctgcac gatgcggcac ggctcgccag cggtttttac attgcagtga
     9181 cactcgtttg cattggtctt gccgggcgcg cgctctacgg acgcggccaa ggcacgatga
     9241 gcgcgttgct gttgttgggc tgcctgggtc tgctagtgcg tgcgcaccag ctcgtttccg
     9301 atattggcct gcttgccggt tttgcgctct gcctctacgg cctggcctta aatcgcgaac
     9361 acccgttgca ggctgggttg cttaccggaa ccggcgtggg cctcggcttc atgagccggg
     9421 gaacgctggc tccgatggtg atggcgctca cctgcctgct gctgccgctg ttgtttcgtg
     9481 aatggcgcga ccgccgttat gccttgacca ttctcatcgc ggcaattgcc agcgcgccgt
     9541 ggctgattat ctggccttac gccctctatc agcactctcc ggagctttac caccaatggc
     9601 tgtgggttca ggacttggag cgcctgattg gtcttgctcc ggccggcaac gaagcttcgg
     9661 cctggtattt tctgaaaacc ctaccgtggt ttgcttggcc ggcgctgcca ctcgctctgt
     9721 ggactttgtg gcacgggagg cgcaaaattt tcaaccgcgc ggaaattcaa ttgccgctgg
     9781 tgctgttggt cgcgctgtat gccgtgctaa gcctcgatac cgatacccac gatctggatg
     9841 cgttgccact gcttttgccg attgtgctac tcgccacgcc cgccctggga actttgacgc
     9901 gcagcgcggc caacgcgctc gactggttcg gcatcatgac ttttggcttg ctcgcgctcg
     9961 gcctgtggct gggttggcta gcgttgatga ccggttcccc cgccggtatc gccacccggc
    10021 ttaacgagta tttgccggga tacaacctgc cgtttaatct cttccatttt ttaatcgcgg
    10081 ttgcgttcac cctcggatgg ctgtcgttaa tcagccgcat cgggcgctcc aaccgccgcg
    10141 ccgtcatcaa ctgggccggc ggcatcacca tggtgtggat gctggcgatg accctgtggc
    10201 taccctggct ggatgtaagc aagaactacc gctggatgat ggcctcgctg cagcagtcgc
    10261 tgccggcgga attccagtgc atatccagcc gcggactggg caagacacag cgttccatgc
    10321 ttgagtatta caccggcatc attacccggc gtatggaact ctccggtacc gccgactgcg
    10381 atttgctgtt gattcaaagc agatcgcagg agactccctc ggttagtccg ggttgggaaa
    10441 aaatctggga aggcagccgt ccaggtgacc tcaacgagcg ttaccgcctc taccagaagc
    10501 aggaacgcag ttacgcaact caaggctgat ggcaaagtgc ctcgacaggt gcaagttatc
    10561 agggtaaact ttaattgtgc agtgcatc
//
LOCUS       DATIOW010000216         6603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_295957, whole genome shotgun sequence.
ACCESSION   DATIOW010000216 DATIOW010000000
VERSION     DATIOW010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6603
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295957"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VLL03_07190"
     CDS             complement(<1..191)
                     /locus_tag="VLL03_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS47180.1"
                     /translation="MSERNTEQPPVIVVPGLFGSKLRDRATGKEVWPGSTLDIVFGKY
                     EDLGLEIDPDTLLPKPSSL"
     gene            complement(300..1004)
                     /locus_tag="VLL03_07195"
     CDS             complement(300..1004)
                     /locus_tag="VLL03_07195"
                     /inference="COORDINATES: protein motif:HMM:NF016367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HSS47181.1"
                     /translation="MTRLLVSVKSLDEARMALECGADIIDFKDPANGALGVLPSDTVR
                     ETAQWINRRCPVSATLGDLPMEPATLVQAVRKAAAGVDFVKIGFFPHGDSRICIHALA
                     RQARRVGLVAVLFADCNPDFSLLEALAQAGFAGVMLDTADKTQGGLRRHWDDAGLNNF
                     VKRAQRLGLIAGLAGSLRLEDVSDLIQLGPDYLGFRGALCARTQRNSGLQASRVSALR
                     HAIPRLSAESSMAVGG"
     gene            complement(1001..1576)
                     /locus_tag="VLL03_07200"
     CDS             complement(1001..1576)
                     /locus_tag="VLL03_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 domain-containing protein"
                     /protein_id="HSS47182.1"
                     /translation="MIFETIVTTQAQDRRIHIAPMGIREESGLIVLAPFRPSTTLDNV
                     LANGHAVVNSCDDVRIFAGCLTDRRDWPTVPADKVPGIRLAAALAHWELELEKMEEGK
                     MRPRLLCRVVHQASHSPFRGFNRAQAAVLEAAILVSRLHLLPWEKIEAEVKYLAIAID
                     KTAGPREQVAWGWLVEKIEAHCATLKHSSAA"
     gene            complement(1573..2979)
                     /locus_tag="VLL03_07205"
     CDS             complement(1573..2979)
                     /locus_tag="VLL03_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6513 domain-containing protein"
                     /protein_id="HSS47183.1"
                     /translation="MSERILFLTGKLAEKSLHKVLAEMHPSEFGYDVLNIGISVAGLM
                     TADLILRRLPNPGNSTRIMVPGRCRGDLDALSKRYGVPVARGPEELKDLPQFFGKKGR
                     EQDLSRYDVKIFAEIVDAPQSSIEQILKRAERYRKDGADVIDLGCLPETPFPHLEEAV
                     AALKKTGFAASVDSVEPEELLRGGKAGADYLLSLKEDTLWVADEVASIPVLIPAQPGD
                     LASLERAMEGMMKRNRRFIADSVLDPIHFGFADSLVRYHELRRRHPEIEIMMGIGNLT
                     ELTDADTTGINALLMGVISELRITHILTTEVSPHARRAVREADAARRMLYAAREAGSL
                     PRDFSSALLTLHSRKPFPDTPQEIAELSRAIKDPSYRIQVSDQGIHIYNRDGMHVAAD
                     PFALFPLLDLQNDPAHAFYLGVELARAQIAWQLGKRYNQDEELDWGCAVERKPQDLMQ
                     HHAPGTTLQNRKKKTGNQ"
     gene            complement(3012..3563)
                     /locus_tag="VLL03_07210"
     CDS             complement(3012..3563)
                     /locus_tag="VLL03_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HSS47184.1"
                     /translation="MHDHKPRLAWALTGSGHYLKESLDLALQLPHVDLYLSKASAEVV
                     HMYAHSLKELRARFRVFRDTTASAAPVGLFYYGHYHTMVIAPATSNTVAKCVWGISDT
                     LVTNIFAQAGKCRIPSIVFACDTEPMVTTEAPKEWVTLYPRKIDLDNMEKLKSFENVT
                     VVSRIEELREAVEARMKTLGIKA"
     gene            complement(3565..5520)
                     /locus_tag="VLL03_07215"
     CDS             complement(3565..5520)
                     /locus_tag="VLL03_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HSS47185.1"
                     /translation="MSDLITTHAKFIHSVLKGAVKIDDERITRSWRRCIDDYHLDPAS
                     TRDPVVVDRSELIERQERMADVLSIAKIEMTNLYQQLAGSGYAIMLTDVDGVLLNYFG
                     DPEFTHTASKTGLMLGAVWSEQYQGTNGMGTCLLEKKPLIVHHREHFLARNIGLTCSA
                     APIFDHSGKLLAVLDASGESRLAQQHTLVLVNMSAQMIENRAFLSSFSRDYIVRFHSR
                     PEFVGTLGEGAIAFTGEGRVLAANHSALFQLGYQEPRDAVGKDIGELFNTSLHSLVEL
                     SARNSFHPMPIFEARRGSRFFAVAQQPEGAKPLAPLVATVVRGLRKTTRSNAATTPLL
                     DELAFGDPLLEKNIGAAKRILSRDVALLLYGETGTGKEMFAKALHYSSNRADKPFVAI
                     NCASIPETLIESELFGYKAGAFTGASREGQRGKIFQANGGTLFLDEIGDMPIQLQARL
                     LRVLEEREILPLGGEVPVKVDIRLISATHSNLADKIIKGEFRQDLYYRLHGLCLTLPP
                     LRERTDKCELIKHILAAENPDDQYVEIDDAALGQLEHYLWPGNIRQLRNVLRTVLALR
                     EGDVITVKDLPQEIVGCRSHARENGAAAAALNPLESAERDALVRELGNHHWNITSVAR
                     QLKISRNTLYRKMQRLNIKDPNKDSSQ"
     gene            5712..6044
                     /locus_tag="VLL03_07220"
     CDS             5712..6044
                     /locus_tag="VLL03_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS47186.1"
                     /translation="MRKYLVLGLMLLSGSTWAHEYPASEVVEFVLQCMQKHPGKFEYL
                     YKCSCVMDTFAKQLKYDDYVELFTASRGQSTTGEKGGLFRDPPEIKEMANRYNAMKTQ
                     AEKECLFQ"
     gene            6065..6556
                     /locus_tag="VLL03_07225"
     CDS             6065..6556
                     /locus_tag="VLL03_07225"
                     /inference="COORDINATES: protein motif:HMM:NF023113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3280 domain-containing protein"
                     /protein_id="HSS47187.1"
                     /translation="MLCAFSAPCAATQQLASLAVLDFELIDDTQDKNPETLATQQKRL
                     ALVSEQLRKEFNKAGLYRVLDNAPAADFIARLKTSQELHSCNGCEIDIGKRLGADFVL
                     TAWVQKVSNLILNLNIEIKNVATGQKVLNKSVDIRGNTDQSWSRGITYMVRDMVEKKQ
                     CGR"
BASE COUNT         1283 a         1884 c         2057 g         1379 t
ORIGIN      
        1 tccagcgacg aaggcttggg aagcagggta tccggatcga tttccaagcc taggtcttcg
       61 tatttgccga acacgatgtc gagcgtcgag cccggccaga cttcttttcc cgtggcgcga
      121 tcgcgcagct tggagccgaa caggccgggc accacgatca ccggcggctg ctcggtattc
      181 ctttcgctca tctgatacag gcgtttgaag tcgggccgta tcacttccgt gcaagccgca
      241 agcgacaggc aaagcagcag taagggaacc agaaaaggtc gcatggggat gcgtcccaat
      301 catccgccga ccgccatact gctttctgcc gacaagcggg gaattgcatg gcgcaaggcg
      361 gagactcggg aagcctgcaa accgctgttt ctttgcgttc tcgcgcacag ggcgccgcgg
      421 aaccccaggt agtcggggcc aagctggatg agatcggaaa cgtcttcgag ccgcagcgat
      481 ccggcaagac cggcgatcaa accaaggcgc tgcgctcttt ttacgaaatt attcaagccg
      541 gcgtcgtccc aatgccggcg cagcccgcct tgagttttgt ccgcggtgtc gagcatgacg
      601 ccggcaaaac ccgcttgcgc caaagcttcg aggagggaga aatccggatt gcaatccgcg
      661 aacagcacgg cgacgaggcc aacccgccgc gcctggcgcg ccagcgcatg gatgcagatg
      721 cgggaatcgc cgtgcgggaa aaacccgatc ttcacaaaat ccacgcccgc cgccgctttc
      781 ctgaccgctt gcaccagcgt cgcgggttcc atcggcaggt cgcccagggt ggcgcttacc
      841 gggcagcggc ggttgatcca ttgcgccgtt tcccgaacgg tgtctgaagg cagaacgccc
      901 agcgcgccgt ttgccggatc tttgaagtcg atgatgtcgg cgccgcactc cagggccatg
      961 cgcgcttcat ccagcgattt cacgctcacc agaagtcggg tcatgcggcg ctgctatgct
     1021 tgagtgtcgc gcagtgcgct tcgatttttt ccacgagcca gccccaggct acttgttcgc
     1081 ggggtcccgc ggtcttgtcg atggcgatgg cgaggtattt cacttcggct tcgatttttt
     1141 cccagggcag cagatgcaag cgcgagacca ggatcgctgc ttccagcacc gccgcctggg
     1201 cgcggttaaa gccgcgaaac ggcgagtgcg aggcctggtg caccacccgg cataaaaggc
     1261 ggggtcgcat tttgccctct tccatttttt ccagctccaa ttcccagtga gccagcgccg
     1321 ccgcgagcct gatgcccggc accttgtccg caggaacagt cggccaatcg cggcggtcgg
     1381 tcaggcatcc ggcgaaaatg cgcacgtcgt cgcaggagtt gaccacggca tggccgttgg
     1441 cgagcacatt gtccaaagtg gtggaggggc ggaagggcgc gagcacgatg aggccgcttt
     1501 cctcgcgaat tcccatcggc gcgatgtgga tgcggcggtc ctgcgcctgg gtggtgacga
     1561 tagtctcgaa tatcattggt tgccggtttt ctttttgcgg ttctgcaggg tcgttccggg
     1621 tgcgtggtgc tgcatcaggt cctgaggctt acgctccacc gcgcaacccc agtccagttc
     1681 ctcgtcctga ttgtaacgct tgccgagctg ccaggcgatt tgcgcgcgcg ccagctccac
     1741 gcccagataa aacgcgtgcg ccggatcgtt ttgcaagtca agcagaggaa acagcgcgaa
     1801 ggggtcggcg gccacatgca ttccgtcgcg gttgtagatg tgtatgccct gatcgctcac
     1861 ctggatgcgg tagctcgggt ccttgatggc gcgggacaat tcggcgattt cctgcggcgt
     1921 gtcgggaaac ggcttgcgcg agtgcaaggt gagcagcgcg gaagaaaaat cgcgcggcag
     1981 gctcccggcc tcgcgcgcgg catacagcat acgccgcgcg gcatcggctt cgcgcactgc
     2041 gcgccgggcg tgcgggctca cttcggtggt gaggatgtgg gtgatgcgca attcggaaat
     2101 aacgcccatc agcagcgcgt tgatgccggt ggtgtcggcg tcggtgagct cggtgaggtt
     2161 gccgatgccc atcatgattt caatctcggg gtggcggcgg cgcaattcgt gatagcgcac
     2221 cagagaatcg gcgaaaccga aatgaatcgg gtcgaggacc gaatcggcga tgaagcggcg
     2281 gttacgtttc atcatgcctt ccatcgcgcg ttccaatgaa gccaaatctc cgggctgcgc
     2341 cggaatcagc accggaatgg aagcgacctc atcggcgacc cacagcgtgt cttccttgag
     2401 gctgagcaaa taatccgcgc cggctttgcc gccgcgcaat aattcctcag gctcgaccga
     2461 atcgacgctt gccgcaaaac ccgttttttt cagcgcggcg accgcttctt caaggtgggg
     2521 aaacggcgtt tccggcaggc agccgagatc gatcacatcg gcgccgtcct tgcggtagcg
     2581 ttccgcgcgc ttgaggattt gctcgatgct tgactgcggc gcgtcaacga tttcggcgaa
     2641 gattttcacg tcgtagcggc tcaagtcttg ttctctgcct ttttttccga aaaactgggg
     2701 gaggtctttg agttcttccg gcccgcgcgc aaccggcacg ccgtagcgtt ttgaaagcgc
     2761 gtcgaggtcg ccgcggcagc ggcccggcac catgatgcgg gtcgagttgc cgggattggg
     2821 caggcgccgc aggatcaggt ctgcggtcat taatccggcg acgctgatgc cgatgttgag
     2881 cacgtcgtag ccgaactcgc tcggatgcat ctccgccagc accttgtgca ggcttttttc
     2941 ggcgagtttg ccggtgagaa ataaaatccg ttctgacata cgcttaatat actccactgg
     3001 caccggcagc ttcaggcttt gatgcccagc gttttcatcc gcgcctcgac cgcctcgcgc
     3061 aattcctcga tgcgcgaaac cacggtgacg ttttcaaagc ttttcagctt ttccatgttg
     3121 tcgagatcga ttttgcgcgg atacagcgtc acccattcct tcggcgcttc ggtggtgacc
     3181 atcggctcgg tgtcgcaggc aaagacgatg ctgggaatgc ggcatttgcc ggcctgcgca
     3241 aagatgttgg tgaccagggt gtcggaaatg ccccacacgc atttagccac ggtgttcgag
     3301 gtggcgggcg caatcaccat ggtgtgatag tggccgtaat agaacaatcc gaccggagcg
     3361 gcactggcgg tggtgtcgcg gaatacgcga aaacgggcgc gcagctcttt caggctgtgg
     3421 gcgtacatgt gcacgacttc ggcggacgcc ttggacaaat acaggtccac gtgcggtagt
     3481 tgcagcgcga ggtctagcga ttccttgagg taatggcctg agccggtcag cgcccaggcg
     3541 agacggggct tgtgatcatg cacgctattg cgaggaatcc ttgttgggat ccttgatgtt
     3601 cagccgctgc atcttgcggt acagggtgtt gcggctgatt ttgagctgcc gtgccacgct
     3661 ggtgatattc cagtgatggt ttcccagttc gcggacgagg gcgtcgcgtt ccgcggattc
     3721 gagcgggttg agcgccgctg ccgcagcgcc gttttcacgc gcgtgcgatc ggcagccgac
     3781 gatttcctgc ggcaggtcct ttacggtgat cacgtcgcct tcgcgcagcg ccagcacggt
     3841 gcgcaacacg tttcgcagct ggcggatatt gcccggccac agatagtgtt ccagctggcc
     3901 cagtgccgca tcgtcgattt ccacatactg gtcatcgggg ttttcggcgg cgaggatgtg
     3961 cttgataagt tcgcacttgt cggtacgctc cctgagcggg ggcagtgtca ggcacaggcc
     4021 gtgcagacgg taataaagat cctgacggaa ttcacctttg atgattttgt cggcaagatt
     4081 gctgtgggtg gcggaaatga gccggatatc tactttaacc ggaacttcac cgcccaaagg
     4141 caggatttcg cgttcttcca gcacgcgcag caagcgtgcc tgcagttgta tcggcatgtc
     4201 gccgatttca tcgaggaaca gcgtgccgcc attggcctgg aaaatcttgc cgcgctggcc
     4261 ctcgcggctc gcgccggtga acgcgcccgc cttgtagccg aacagttccg attcgatcag
     4321 cgtctccggg atagatgcgc aattgatggc gacaaacggc ttatcggcac ggttgctgga
     4381 atagtgcagc gccttggcaa acatttcctt gccggtgccg gtctcaccgt agagcagcag
     4441 cgcgacgtcg cggctgagga tgcgcttggc ggcgccgata tttttttcca gcagcgggtc
     4501 gccaaaagcc agttcatcca gaagcggcgt ggtggcggcg ttggagcgcg tggtcttgcg
     4561 cagtccgcgt acgacggtgg cgaccaatgg cgcaaggggt tttgcgcctt ccggctgctg
     4621 cgccacagcg aaaaagcggc tgccgcgccg cgcttcgaaa atcggcatcg gatggaacga
     4681 attgcgcgcc gagagttcga ccagcgaatg cagggaggtg ttaaacagtt cgccgatatc
     4741 cttgcccact gcatcgcgcg gctcctggta gcccagctgg aacaatgcgc tgtggttcgc
     4801 cgccagcacg cggccttcgc cggtgaaggc aatcgcgcct tcgcccaagg tgccgacgaa
     4861 ttcggggcgg ctgtggaaac gcacgatgta gtcgcgcgag aaactcgaca ggaacgcccg
     4921 gttttctatc atctgcgccg acatgttgac cagcaccagg gtatgctgct gcgcgaggcg
     4981 cgactcgccg gaggcatcga gcaccgcaag gagttttcct gagtggtcga aaattggcgc
     5041 ggcggagcag gtcaagccga tgttgcgcgc gagaaaatgc tcgcggtggt gcacaatcag
     5101 cggttttttc tcgagcaggc aggtgcccat gccgttggtg ccctggtatt gctcgctcca
     5161 aaccgcgccc agcatcagcc cggttttcga tgcggtgtga gtgaactccg gatcgccgaa
     5221 gtagttcaaa agtacaccgt caacatcggt aagcatgatg gcgtaaccgg aaccggcgag
     5281 ctgctgataa aggttggtca tttcgatttt ggcgatggag agcacgtcag ccatgcgctc
     5341 ctggcgttcg attagttcgc ttctgtccac cactaccgga tcgcgggtgc tggcgggatc
     5401 aaggtggtaa tcgtcaatgc agcgccgcca cgagcgggtg atgcgctcgt cgtcaatctt
     5461 cactgcgccc ttgagcacgg agtgaatgaa tttagcgtgg gtagttatga ggtccgacat
     5521 gccaggttcc gcatttggtt taaggattta cgccgctccc caaggcgctt caaccccgtg
     5581 ttgcagtggg gttttcagct taaacgcagc gagtatgtgt attatagtgc ttaacgctgg
     5641 aagtatgaaa tatgacacgc aacttgcgat tttgcaaaag ttttttgttg ttttagacaa
     5701 ggagtttgca gatgcggaaa tacctggtgt taggcctgat gttgttgtcg ggctcgacgt
     5761 gggcccatga atatccggcg agcgaggtgg tggagtttgt gctccagtgc atgcagaagc
     5821 accccggcaa gtttgagtac ctttacaagt gctcgtgcgt catggacact ttcgccaagc
     5881 aactgaaata cgatgactat gttgagctat tcaccgcctc gcgcggccag agcaccacgg
     5941 gtgaaaaggg cggcctcttc cgcgatcctc ccgagatcaa ggaaatggcc aatcgctaca
     6001 acgcgatgaa aacccaagcc gaaaaagaat gcctgtttca gtgacgcggt ggctgacagt
     6061 cgcattgctt tgtgcattca gcgcgccctg cgccgcgacc cagcagctcg ccagcctcgc
     6121 cgtgctggat ttcgagctga ttgacgacac ccaggataaa aatcccgaaa cgcttgccac
     6181 ccagcaaaaa cggctggcgc tcgtcagcga gcagctgcgc aaagagttta acaaagcggg
     6241 actttatcgc gtgctcgaca acgcccccgc agcggatttc atcgcgcggc tcaagaccag
     6301 ccaggaactc cactcttgca acggctgcga gatcgacatc gggaaaaggc tcggcgccga
     6361 tttcgtgctt accgcctggg tgcagaaagt cagcaacctc attctcaacc tcaacatcga
     6421 aatcaagaac gtcgctaccg ggcagaaggt cctgaataaa tcagtggata tccgcggcaa
     6481 caccgaccaa tcgtggtcgc gcggcatcac ttacatggtg cgcgacatgg tggaaaagaa
     6541 gcagtgtggg aggtaggaat ctctgaatta agttcatcgc gtgcgcgcgg gactcaatca
     6601 gag
//
LOCUS       DATIOW010000217        15231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1313636, whole genome shotgun sequence.
ACCESSION   DATIOW010000217 DATIOW010000000
VERSION     DATIOW010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15231
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313636"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VLL03_07230"
     CDS             complement(<1..472)
                     /locus_tag="VLL03_07230"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HSS47188.1"
                     /translation="MLTDIEIAQKARMQRITQLAKGKLGIDEEHLEPYGHYKAKLSLD
                     FVDSLANKPDGKLILVTAISPTPAGEGNTTTSVGLGDALNRIGKKAVICLREPSLGPV
                     FGMKGGAAGGGYAQVVPMEDINLHFTGDFNAIGLANNLLAAMLDNHIHHGNALGL"
     gene            complement(612..3410)
                     /gene="fdhF"
                     /locus_tag="VLL03_07235"
     CDS             complement(612..3410)
                     /gene="fdhF"
                     /locus_tag="VLL03_07235"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HSS47189.1"
                     /translation="MNAPVEQIVKPEVVEFKLDGKSVTGFSNETIIETAKRYGVEIPH
                     LCYSPGLRPDGNCRACMVEIKGERILAPSCCRTPMKGMEVFSDNPRAQASQKMVLELL
                     LSDMPQQSYTINSELDLWVKKLKLGKPRFSPRQQPAADVSHPAIAVNLDACIQCTRCL
                     RACREEQVNDVIGYASRGEYSKIVFDLDDPMGESTCVACGECVQACPTGALMPARGVG
                     MLKPDKTVDSVCPYCGVGCQLTYHVKDNKILYVQGRDGPANHGRLCVKGRYGFDYVSH
                     KQRLTKPLIRRPGFPKSAAFVMDPEDVLKVFREASWKEALELATGNLKKIRDRDGPHA
                     LAGFGSAKGSNEEAYLFQKLVRTGFGSNNVDHCTRLCHASSVAALLEGIGSGAVSNQV
                     SDVLKAGVVFVIGANPTVNHPVAATWIKNAVKKGVKLIVADPRRSELARHAAYFLQFK
                     PDTDVALLNAMMHTIIHEDLANQEFIKNRTSGYEELKKNVEGYSPEAMAPICGIPAAT
                     IKEVARLYATSKGSMILWGMGISQHVHGTDNARCLIALSLLTGQVGRPGTGLHPLRGQ
                     NNVQGASDSGLIPMVYPDYQPVDNANVRAAFEKLWGIKLDPKPGLTVVEIMNAVHAGQ
                     IRGMYIMGENPAMSDPDLGHARAALAELEWLVVQDLFLTETAYLADVILPASAWPEKT
                     GTVTNTDRMVQLGRQALDLPGEAKHDLWIIQEIARRMGLNWNYPGPKDVFNEMRKAMS
                     SIAGITWERLERDSSVTYPCEHEGDPGEPVVFTENFPTPSGRGKFVPADIIPANERPD
                     RDYPLVLITGRQLEHWHTGSMTRRAGVLDAIEPEPVASLHPLDLDAIGAQPGEVITVE
                     SRRGEISLYARADDGTPRGTVFIPFCFYEAAANLLTNPALDPVGKIPEFKYCAVRVKH
                     GGQKPEHASFGGGQVLTL"
     gene            complement(3523..5211)
                     /locus_tag="VLL03_07240"
     CDS             complement(3523..5211)
                     /locus_tag="VLL03_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HSS47190.1"
                     /translation="MEAQKRAGNRPQGGTARATPKGRMVDPAALAEIHALLGNESLRR
                     DLLIEHLHKIQDRYGHIAARHIVALASEMKLSLTEVYEVATFYHHFDVIKEGDTVPPA
                     LTVRVCDSLSCELAGAQNLLAELRQKVGANVRVIPAPCVGRCEQAPVAVVGRNPVPHA
                     NLSQVQAAVNAKTTRCEVASYISYDEYRKQGGYQLLVDCVKGDHESESVIKIMENSGL
                     RGLGGAGFPAGRKWRIVRAEPAPRLMAVNIDEGEPGTFKDRYYLERDPHRFLEGMLIA
                     AWTVEIREIYIYLRDEYAGCREILGTELANLTTHPPCELPQIHLRRGAGAYICGEESA
                     MIESIEGKRGMPRLRPPYVAQVGLFGRPTLEHNMETLYWVRDILEKGADWFSSHGRHG
                     RKGLRSFSVSGRVQKPGVHLAPAGITVKELIDEFCGGMLPGHEFYAYLPGGASGGILP
                     ASMGDIPMDFDTFNEYGCFIGSAAVIILSDRDRAAAAARNLMKFFSEESCGQCTPCRV
                     GTAKALKLIDEPQWNQALLEELSTAMADASICGLGQAAPNPLRCVMKYFPNEIR"
     gene            complement(5512..6387)
                     /gene="sucD"
                     /locus_tag="VLL03_07245"
     CDS             complement(5512..6387)
                     /gene="sucD"
                     /locus_tag="VLL03_07245"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HSS47191.1"
                     /translation="MAILINKNARVMTQGITGKTGQFHTRMCRDYANGKNCFVAGVNP
                     KKSGEDFEGIPIYATVKEAKEKTGAMISVIYVPPPFAAAAIDEAVEADLDLVICITEG
                     IPVRDMIRTRNSMRGRKTVLIGPNCPGVITPDEIKIGIMPGHIHKKGRVGVVSRSGTL
                     TYEAVGQLMEQGLGQSTCVGIGGDPVNGLKHIDVMKMFNEDPDTDAVVMVGEIGGTDE
                     EDCARWIKANVQKPVVGFIAGVTAPPGKRMGHAGAIISGGKGTATEKLAVMEECGIKV
                     TRNPAEMGKLLKSVL"
     gene            complement(6390..7556)
                     /gene="sucC"
                     /locus_tag="VLL03_07250"
     CDS             complement(6390..7556)
                     /gene="sucC"
                     /locus_tag="VLL03_07250"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HSS47192.1"
                     /translation="MKIHEYQGKEILRKYGVATPRGIACFSVDEAAKAAQQIGGKVWV
                     VKAQIHAGGRGKGGGVKVAKSLDEVKQYAEQILGMRLVTHQTGPEGRLVKRLLIEEGA
                     DIKKELYVGMVVDRGMQRVVLMASSEGGTEIEEVAAKTPEKIHKVFIDPMRGLTDKDA
                     EDVARKIGIPEAAVAQARDFLKGLYRVFDETDASLAEINPLILTGDNRVLALDAKMNF
                     DSNALYRHPEIVAMRDLDEEDPAEIEASKFDLSYIPLDGNIGCLVNGAGLAMATMDIA
                     KLYGGSPANFLDVGGGATTEKVAEAFKIMLRNPKVKAILVNIFGGIMKCDVIAEGIVA
                     AAKQVRLSTPLVVRLEGTNVDLGKKILAESGLLIISGTNMADAAQKVVAAAKGK"
     gene            complement(7571..9865)
                     /locus_tag="VLL03_07255"
     CDS             complement(7571..9865)
                     /locus_tag="VLL03_07255"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF009501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HSS47193.1"
                     /translation="MPDKLTESALHYHRFPTPGKTEVIATKPLATQADLALAYTPGVA
                     VPSLEIAEDPNTVFNYTGRGNLVAVITNGTAVLGLGPIGPLAAKPVMEGKGVLFKKFS
                     GINVFDIEINERDPDKLVDIIASLEPTFGGINLEDIKAPECFEIERKLRSRLKIPVFH
                     DDQHGTAIIVGAAVLNGLRVVGKDIAKVKLVCSGAGAAAIACLDLLVSLGLRKENIIV
                     TDTKGVVHEARAEQMDPNKARYAVKTKARTLGEAIVDTDIFLGLSGPGVLKPEMVKQM
                     AKQPLILALANPEPEIRPEVAKEARSDVVIATGRSDYPNQVNNVLCFPFIFRGALDVG
                     ATTINEDMKLAAVRALADLAHAEQSDIVAMAYGEQSLSFGPEYLIPKPFDPRLILKIA
                     PAVAKAAMDSGVATRPIEDFIAYREQLTQFVYRSGLIMKPVFAAAKQSPKRLVYAEGE
                     DERVLRAVQQVVDEGLAQPILIGRPAVVNERIQKLGLRIRPGVDFELINPENLPQYQE
                     YWNTYYNIMQRRGVAIEYARREVRRRTTLIGALMVRMSAADAMLCGTFGHYQLHLDFV
                     MDTLGLRSGVHNAAAMNMLLHPKGTFFICDTYVTPDPTAEQIAEMTILAAEEVQRFGM
                     TPKVALLSHSSFGSVDTPSSRKMREALRLLEKQAPNLEVEGEMHGDAALSEDIRNQVF
                     PNSRLRGRANLIIMPTLDAANISFNLLKVLGDGITVGPILLGCAKPVHILTPTATVRR
                     IVNMTALAVVDANAERFEQASLKL"
     gene            complement(9886..9961)
                     /locus_tag="VLL03_07260"
     tRNA            complement(9886..9961)
                     /locus_tag="VLL03_07260"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9926..9928),aa:Thr,seq:tgt)
     gene            complement(10046..10441)
                     /locus_tag="VLL03_07265"
     CDS             complement(10046..10441)
                     /locus_tag="VLL03_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012763106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HSS47194.1"
                     /translation="MKNISSKPYLIRALCEWCADSGLTPYLAVKVNEHTRVPMEYVKN
                     GEIILNISRDATRNLTLGNELIQFSARFDGVSRELSVPLDAVNGVFAKENGQGLFFND
                     APAAAEQKTGEDNPSPSTPNGKPRLQVVK"
     gene            complement(10712..11311)
                     /locus_tag="VLL03_07270"
     CDS             complement(10712..11311)
                     /locus_tag="VLL03_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HSS47195.1"
                     /translation="MMTLYSGTTCLFSHRCRIVLFEKGMDFQIVDVDLYNMPEDLAVM
                     NPYNRVPVLVERDLILYEANIINEYIDDRFPHPQLMPADPVMRARARLFLFRFELELF
                     SQIETIEHGNQKTADKVRLVIRDNLTQIAPVFAKHKYMLGDEFSMLDVAIAPLLWRLD
                     HYGIQLGKPAALLMKYAERLFSRPAFIDALTAPERAMRK"
     gene            complement(11363..12091)
                     /locus_tag="VLL03_07275"
     CDS             complement(11363..12091)
                     /locus_tag="VLL03_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HSS47196.1"
                     /translation="MKKLLLALMLVPVAALASSDAKYDTAPVNQHDKISLQRGARIFV
                     NYCVTCHNAAYMRYNRLQDLGLTEQQIKDNLMFAVAKRVGDTMTVAMDKSDAKDWFGA
                     PPPDLSLIARVRGADWLYTYLRSFYRDDSTHTGWNNTVFPLVGMPHVLHELQGVQMLK
                     TEEKTDSQGHQHEDHKLILEKPGVLTPQQYDMLVADLVNYLVYMAEPARNFRVQLGMF
                     VLIFLGILFVLTYALKKEFWKDVH"
     gene            complement(12094..13455)
                     /locus_tag="VLL03_07280"
     CDS             complement(12094..13455)
                     /locus_tag="VLL03_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HSS47197.1"
                     /translation="MTKKLTMLLGWIDQRFPLTASWKTHLSEYYAPKNFNFWYFFGSL
                     ALLMLVLQIVTGIFLTMNYKPDASLAFASVEFIMRDVPWGWLIRYMHSTGASMFFVVV
                     YLHMFRGLLYGSYRKPRELIWIFGMLIYLCLMAEAFFGYLLPWGQMSFWGAQVIVNLF
                     DAIPLIGPDLALWIRGDYAVSDATLNRFFAFHVIAVPLVLLGLVAAHLIALHEVGSNN
                     PDGIEIKKNKDPKTGIPLDGIPFHPYYTVKDMVGAVVFLFVFSIAVFFAPEMGGYFLE
                     ANNFIPANPLQTPEHIAPLWYFTAFYSILRATTEDFMWFLRVGLVVYMILAIIGVKQN
                     HFKFTIAIIGALIFVATFVFEAKIWGVALMGISVMIFILLPWLDQSPVKSIRYRGALY
                     KTALALFVISFLVLGLLGTQPVSRLYTIIVQICTLIYFAFFLLMPWYTRFDKTKPEPE
                     RVR"
     gene            complement(13458..14054)
                     /gene="petA"
                     /locus_tag="VLL03_07285"
     CDS             complement(13458..14054)
                     /gene="petA"
                     /locus_tag="VLL03_07285"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HSS47198.1"
                     /translation="MTDNNQVDRSKRRFLIAATTVAGGVATAATAVPFVVSMLPSQRA
                     LAIGAPVEVDIGKIEPGAKLDLEWQGKVVWIVNRTKEMLELLPKLNDKLADPDSESSE
                     QPGYCKDLTRSLKPQYFIAIGLCTHLGCSPTYRKEVGAPDLGADWPGGFFCPCHGSRF
                     DLAGRVFKNVPAPTNLRIPPHRYLSDNKILIGEDSKGA"
     gene            14418..14789
                     /locus_tag="VLL03_07290"
     CDS             14418..14789
                     /locus_tag="VLL03_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HSS47199.1"
                     /translation="MARYVESNLIRDEHIVYETKLHWITFVSLKALLTLFIAPLIARA
                     TSEFAITNKRIIIKVGLISRHTLEMNLSKVESVAVDQGICGRILRYGSITIIGTGGTK
                     ELFAYLANPVEFRRQFQQLQA"
     gene            14921..>15231
                     /locus_tag="VLL03_07295"
     CDS             14921..>15231
                     /locus_tag="VLL03_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-alkenal reductase"
                     /protein_id="HSS47200.1"
                     /translation="MRKLWLIFAQTATVCLAVVFVVTTLKPELLPAGWRGGNVVSFRE
                     AAPDVTTKKINSYSDAAKKAMPSVVNVFTSKEVRVPRHPLMDDPLFRHFFGDRGDEKR
                     R"
BASE COUNT         3203 a         4432 c         4245 g         3351 t
ORIGIN      
        1 ccagccccag cgcatttccg tggtgaatgt gattgtccag catcgccgcc agcagattat
       61 tcgcaaggcc aatggcgttg aagtcgccgg tgaaatgtag attgatatcc tccatcggca
      121 ctacttgcgc gtaaccgccg cccgcagcgc cgcctttcat cccaaacacc gggccaagag
      181 aaggctcacg caggcaaatc accgcttttt tgccgattcg attcaaggcg tcacccagcc
      241 ccacggaagt ggtggtgttg ccttcgcccg cgggtgtagg actaatagca gtaaccagaa
      301 tcagcttgcc atcaggctta ttagcgagac tgtcaacaaa atccagggat agcttggcct
      361 tgtagtggcc ataaggctcg agatgttctt cgtctatccc gagttttccc ttcgccagtt
      421 gggtaatgcg ctgcattctg gctttttgcg cgatttcaat gtccgtcagc atgaatccac
      481 cctttctttg aaaaacgatt ttccccctaa tccgcttatg attaagacag cttgcagggc
      541 atgttctgat ccggtaagcc ccgggtcgct atggcgggaa atggctcgac ccgggtcgaa
      601 ttactggcgg attatagggt gagtacctgt ccgccaccaa aacttgcgtg ctcgggcttc
      661 tgcccaccgt gttttactct aaccgcacag tacttgaatt cgggtatttt gcccacaggg
      721 tcgagcgccg gattggtcaa cagatttgcc gccgcctcgt agaagcagaa gggaataaac
      781 accgtgccac gcggcgtgcc gtcgtcggcg cgcgcataga gcgaaatttc cccgcgccgt
      841 gattccaccg taattacctc gcctggctgc gcgccgatgg catccaaatc caaaggatgg
      901 agtgaagcca ccggttcggg ctcaatcgca tcgagcacgc cggcacggcg cgtcatgctg
      961 ccggtatgcc agtgctcaag ctgccgcccg gtaatcagca ccagcggata atcccggtcc
     1021 ggccgctcgt tggcgggaat aatatccgcc gggacaaact tgccgcgccc gctgggcgtc
     1081 gggaaattct cagtgaacac caccggctcg ccgggatcgc cttcgtgctc gcacggatag
     1141 gtcaccgagc tatcacgctc aagccgctcc caggtaatcc ccgcgatcga agacatcgcc
     1201 ttgcgcattt cattgaacac gtccttcggc cccgggtaat tccagttgag ccccatgcgt
     1261 cgcgcgattt cctgaatgat ccacagatcg tgcttggcct cgcccggaag atccaacgcc
     1321 tgccggccga gctgcaccat gcggtcggtg ttggtgaccg tgccggtttt ctccggccac
     1381 gccgaagccg gcaggatgac gtcagcgagg tatgcagttt cggtgaggaa aaggtcctgc
     1441 actactagcc attcaagctc ggccagcgct gcgcgcgcat gcccgagatc cgggtcggac
     1501 atggcggggt tctcgcccat gatgtacatg ccgcggattt gcccggcgtg caccgcattc
     1561 atgatttcca ccaccgtgag gcccggtttg ggatcgagtt tgatacccca cagcttctcg
     1621 aatgcagcgc gcacgtttgc gttgtccaca ggctgataat caggatacac catcggaatc
     1681 agccccgagt cggaggcgcc ctgcacgttg ttctggccgc gcaaaggatg cagtccggtg
     1741 cccggacgcc ctacttgacc ggtgagcagc gagagcgcga tcaggcagcg cgcattgtca
     1801 gtgccatgca cgtgctgcga aatgcccata ccccagagga tcattgaccc tttcgaggtg
     1861 gcgtaaagtc ttgccacctc tttgatcgtt gcggcgggaa tgccgcaaat tggcgccatg
     1921 gcttcagggc tgtagccttc gacgtttttc ttcagttctt cgtaaccgct ggtacggttc
     1981 ttgatgaatt cctggtttgc cagatcctcg tgaatgatgg tgtgcatcat ggcattcagc
     2041 agcgcgacgt cggtgtcggg tttgaactgc aggaagtaag ccgcgtggcg cgcgagttcc
     2101 gagcggcgcg gatccgcgac gatgagcttc acgccctttt tcaccgcatt cttgatccag
     2161 gtcgcagcca ccggatgatt caccgtcggg ttagccccga tcacgaacac cactcccgct
     2221 ttcagcacat cgctgacctg gtttgacacc gcgcccgagc cgatgccttc gagcagcgcc
     2281 gccacacttg aggcgtggca caggcgcgtg cagtggtcca cattgttgct gccgaagccg
     2341 gtgcgcacca gcttctggaa caggtatgct tcttcgttcg agcccttggc cgaaccaaag
     2401 ccggcgagcg catgcggtcc atcgcggtcg cggatttttt tcaaattgcc tgttgcgagc
     2461 tccagcgcct ccttccaact cgcttcgcgg aacactttca gcacgtcttc cggatccatc
     2521 acgaaagcgg cggacttggg aaagcccggc cggcgaatca gcggcttggt gagtcgctgt
     2581 ttatgactta cgtaatcaaa accgtaacgg cctttcacgc acagccggcc gtgattcgcc
     2641 ggaccgtcgc gcccttgcac gtaaagaatc ttgttgtcct tgacatggta ggtgagctgg
     2701 caaccgacgc cacagtaggg gcaaaccgaa tccacggtct tgtccggctt aagcatgccc
     2761 acgccccggg caggcatgag cgcgccggtg ggacaggcct gcacgcattc tccgcacgcc
     2821 acgcaggtac tctcacccat cgggtcatcg aggtcgaata cgatcttgga atactcgccg
     2881 cgggatgcgt agccaatgac atcgttgacc tgctcttcgc ggcaggcacg caggcagcgc
     2941 gtgcactgga tgcaggcatc gagatttact gcaatggcag ggtgcgaaac atcggcggcc
     3001 ggctgctggc gcgggctgaa gcgcggtttt ccgagcttga gtttttttac ccacaggtcg
     3061 agctcggagt ttatagtata ggattgctgc ggcatgtcgg agagaagcaa ctccagcacc
     3121 attttctgcg acgcctgggc ccgcgggttg tcagaaaata cttccatgcc tttcatgggg
     3181 gtgcggcagc aggaaggtgc caatattcgc tcgcctttga tttccaccat gcaggcacgg
     3241 caattgccgt ccgggcgcag accggggctg tagcacagat gcggaatctc cacgccgtaa
     3301 cgcttggcgg tctcgataat agtctcgttg ctgaaaccgg tgacgctttt gccgtccagc
     3361 ttgaattcaa ccacttccgg cttcacaatc tgctcaactg gagcgttcat cgtctctcct
     3421 gccttttacc aggtgaaata agtgaatgaa gtgaatcgcg ctgcgcgctg tgaacaagca
     3481 ggcttggttt taaattcacc tctttcactt cattcacctc cttcaccgaa tttcattcgg
     3541 gaaatatttc atcacgcagc gcaatggatt cggggcggcc tgccccaaac cgcaaatcga
     3601 agcatccgcc attgccgttg agagttcttc gagcagcgct tggttccatt gcggctcatc
     3661 gatgagcttc agcgccttgg cagtgccaac acggcagggc gtgcattgtc cgcacgattc
     3721 ttcggaaaag aatttcataa gattgcgcgc cgccgctgcc gcgcgatccc ggtccgacag
     3781 aataataaca gccgccgagc cgatgaaaca gccgtattcg ttgaaggtgt cgaaatccat
     3841 cgggatatcg cccatcgagg ccggcaaaat cccgcccgat gcgccgccgg gaaggtaggc
     3901 gtaaaactca tgccccggca gcatgccccc gcaaaattca tctatgagtt ccttgacggt
     3961 aatgcccgca ggcgcgagat gtacgccggg cttctgcacg cggcccgaga ccgaaaacga
     4021 gcgcaaaccc ttgcgcccgt ggcggccgtg agaggaaaac cagtcggcgc ctttttccag
     4081 aatgtcgcgc acccagtaca gcgtttccat gttgtgctcc agcgtcggcc gtccgaacag
     4141 gccgacttgc gcgacatacg gcgggcgcag ccgcggcatg ccgcgcttgc cttcgatgga
     4201 ttcgatcatc gccgactctt cgccgcagat gtaggcgccc gcgccgcgcc gcaaatggat
     4261 ttgtggcagc tcacacggtg ggtgggtggt taaattggcg agctccgtgc caagaatttc
     4321 gcgacagccg gcgtattcat cgcgcagata aatatagatt tcgcggatct ccaccgtcca
     4381 cgccgcaatc agcatgccct cgaggaaacg gtgcggatca cgctccaggt aatagcggtc
     4441 tttgaacgtg ccgggctcgc cttcgtcaat gttcaccgcc attaatcgcg gcgccggctc
     4501 ggctctcacg atgcgccact tgcgtccggc gggaaacccc gcgccgccca acccgcgcaa
     4561 gcccgagttt tccataatct tgattaccga ttcggattcg tggtcgcctt tgacgcaatc
     4621 gacaagcaat tgatagccgc cctgtttgcg gtattcgtcg tagctgatat acgaagccac
     4681 ctcacaccgg gtagttttgg cattcactgc ggcttgcact tgactgagat tcgcatgcgg
     4741 cacgggattg cgccctacca ccgcaaccgg cgcctgctcg cagcgtccga cacagggcgc
     4801 aggaatgacc cgtacattcg ctcctacttt ttgcctgagc tcggcgagca gattttgcgc
     4861 gccggcaagt tcgcaggaaa gcgaatcgca cacgcgcacg gtgagcgcag gcggaaccgt
     4921 atcgccttcc ttgatcacat cgaaatgatg atagaaggtt gcgacctcat agacttcagt
     4981 aagggaaagc ttcatctcgg aagccagcgc cacgatgtgg cgcgctgcga tatggccata
     5041 gcggtcctgg attttatgca ggtgttcgat cagcagatcg cgccggagtg attcgttgcc
     5101 aagcagcgcg tgaatttccg ccagcgccgc cggatcaacc atgcgtccct tgggtgtcgc
     5161 gcgcgcagta ccgccttggg ggcggttgcc agcccgcttc tgagcctcca tacctatcct
     5221 ctcgatgcaa aatcctgcga ttacaggcag ttttatacca accaactgct caattttagc
     5281 attttgaatt atagatattt ttttgcacga aaaggagaca ctttgaggaa ttaagtgtgg
     5341 aataagaggg taatattgca aacttagagc actgatttca agagcttgcc catctcagcg
     5401 gggttgcgcg ttactttgat acctcactcc tccatcactt tagcttttgg cttcggccgc
     5461 gcttcgcgct taacttgctt aagcaagtta gcctacaccg acaagttggc cttagagcac
     5521 tgacttgagt aacttcccca tctcggcggg attacgggta acttttatcc cgcactcctc
     5581 cattacggcc aacttttctg ttgccgtgcc tttgcctccg gagatgatcg caccggcgtg
     5641 acccatgcgc tttccaggcg gcgcggtgac gccggcgatg aaaccgacca ccggcttctg
     5701 cacgttggcc ttgatccagc gcgcgcagtc ttcttcgtcg gtgccgccga tttcgcccac
     5761 catcaccacg gcatcggtgt cgggatcctc gttgaacatt ttcatgacgt cgatgtgctt
     5821 caagccattc accgggtcgc cgccgatgcc gacacaagtg gattgcccca gcccctgctc
     5881 catcaattgg ccgaccgctt cgtaagtcag cgtgccggag cgcgacacca cgccgacgcg
     5941 gcctttttta tgaatgtgcc ccggcatgat gccgatcttg atttcatccg gcgtgattac
     6001 gcccgggcag ttgggaccga tgagaacggt tttcctgccg cgcatactgt tgcgggtgcg
     6061 gatcatgtcg cgcaccggta tgccttcagt gatgcaaatc accagatcga gatcggcttc
     6121 caccgcttcg tctatcgctg ccgcggcaaa cggcggcgga acataaatca ccgaaatcat
     6181 tgctccagtt ttttccttcg cttccttgac ggtggcatag atcggaatac cctcaaaatc
     6241 ctcgccggac ttcttcggat tcaccccggc aacgaaacag ttcttgccat tggcgtagtc
     6301 gcggcacatg cgggtgtgga attgcccggt cttgccggtg atgccctggg tcatgactcg
     6361 cgcattttta ttgataagaa tcgccataat tactttcctt tggcggcagc cacaactttt
     6421 tgtgcggcat ccgccatgtt ggtaccggag atgatgagca gccccgattc ggcgaggatt
     6481 tttttgccca gatccacatt ggtgccttcc aggcgcacca ccagtggcgt gctgaggcgc
     6541 acctgcttcg ccgcggcgac gatgccttcg gcaattacgt cgcacttcat tatcccgccg
     6601 aagatgttga ccagaatcgc cttgactttc gggtttctca gcataatttt gaatgcttcg
     6661 gcgactttct cggtagtggc gccaccgccc acgtcgagaa aattggcggg tgaaccaccg
     6721 tagagcttgg caatgtccat ggtcgccatt gccagacccg cgccgttcac caggcagccg
     6781 atgttgccat caagtggaat ataagaaagg tcgaatttcg aggcctcgat ttccgccgga
     6841 tcttcttcgt ccaggtcgcg catcgccacg atttccggat ggcggtaaag cgcattggaa
     6901 tcgaaattca ttttcgcatc tagagccagc acgcggttgt cgccggtgag aatcagcggg
     6961 ttgatttcgg caagcgaagc atcggtctca tcgaacacgc ggtacaggcc tttgagaaaa
     7021 tcccgcgcct gagcaaccgc tgcttcggga atcccgattt tgcgcgctac atcttccgcg
     7081 tctttgtcgg ttaacccccg catcgggtca atgaatacct tgtggatttt ttccggcgtc
     7141 ttcgccgcca cctcctcaat ctcggttccg ccttcggagc ttgccatcag caccacgcgt
     7201 tgcataccgc gatccaccac catgccgacg tagagttcct ttttgatgtc ggcaccctcc
     7261 tcaatcagca ggcgtttgac gaggcgcccc tcgggcccgg tctgatgtgt caccagacgc
     7321 atgccgagaa tctgctccgc gtattgtttg acttcatcga gtgacttcgc cactttgacg
     7381 ccgccgcctt ttccccgccc gccggcatgg atctgcgctt tcaccaccca cactttgccg
     7441 ccgatctgct gagcagcctt ggccgcttcg tcaaccgaaa agcaggcaat gccgcgcggc
     7501 gtggcgacgc cgtatttcct caggatttcc tttccctgat attcgtgtat cttcattgct
     7561 gttcttaata ttagagcttc aggctggctt gctcgaagcg ctcggcgttg gcgtccacta
     7621 cagcgagtgc cgtcatgttg acaatgcgcc gcaccgtggc ggtcggcgtg agaatatgca
     7681 ccggcttggc gcagccgagc aggatcgggc ctacagtgat gccatcgccc agcaccttaa
     7741 gcaggttaaa cgaaatattc gctgcatcca gcgtcggcat gatgatgaga ttcgcccgcc
     7801 ctctcagccg tgaattcgga aaaacctggt tgcgaatgtc ctcggagagc gcggcgtcgc
     7861 cgtgcatttc gccctcgact tcgagattag gcgcctgctt ttccaacagt ctcaacgctt
     7921 cgcgcatttt gcgtgaggac ggcgtatcca cgctgccaaa gctggaatgc gacagcagcg
     7981 ccaccttggg cgtcatgccg aagcgctgca cttcctctgc cgcaagtatg gtcatttccg
     8041 caatctgctc cgcagtcggg tcgggggtga cgtaagtgtc gcagatgaaa aacgtgccct
     8101 tggggtgcaa caacatgttc atcgccgctg cgttgtgcac accagaacgc agtcccagcg
     8161 tatccataac gaaatccagg tgcaattgat agtggccgaa ggtgccgcac aacatggcgt
     8221 ccgccgcgct catgcgtacc atcagcgcgc cgatcagtgt ggtgcgtcgg cgtacttcgc
     8281 ggcgcgcgta ttctatggct acgccgcggc gctgcatgat gttgtagtaa gtgttccagt
     8341 attcctggta ctgcgggagg ttttccgggt tgatgagctc gaaatccacc ccgggccgga
     8401 tgcgcaggcc cagtttttga atacgctcgt taaccactgc gggacggccg atcaggatgg
     8461 gctgcgccaa accttcgtcc accacttgct gcacggcgcg cagcacgcgt tcgtcttcgc
     8521 cttccgcgta caccaggcgc ttcggtgatt gcttcgccgc agcaaacacc ggcttcatta
     8581 tcaggccgga acggtagacg aactgagtga gttgttcgcg gtaggcaatg aaatcttcga
     8641 tgggccgggt ggccacgccg ctgtccatcg ccgctttggc cacggcgggg gcaatcttga
     8701 ggatgagtcg aggatcaaat ggtttgggaa tgagatattc cgggccgaaa cttaagctct
     8761 gctcgccgta ggccatggcc acgatatcgg actgctccgc atgcgcaaga tcggcgagcg
     8821 cacgcactgc ggcgagtttc atatcctcgt tgatggtggt ggcgccaaca tccagcgcgc
     8881 cgcgaaaaat gaacgggaaa cacagcacgt tgttgacttg gttcggatag tcggagcggc
     8941 ccgtggcgat caccacgtcg gagcgtgctt ccttggcgac ttccgggcgg atttccggtt
     9001 ccgggttggc gagcgccaga atcagcggtt gcttcgccat ttgttttacc atttccggtt
     9061 tcagcacgcc cgggccggaa agcccgagga aaatgtcggt gtcgactatc gcctcgccca
     9121 aggtgcgcgc cttggtcttc accgcatagc gcgccttgtt cgggtccatc tgctcggctc
     9181 gtgcttcatg caccacaccc ttggtatcgg tgacgatgat gttttctttg cgcaagccca
     9241 ggctcaccag aagatcaagg caggcaatcg ccgccgcgcc tgcgccggaa caaacgagct
     9301 tgaccttggc gatgtccttg cccaccactc gcaagccgtt cagcaccgcc gcgccgacga
     9361 tgatcgcggt gccgtgctga tcgtcgtgaa ataccggaat tttcaggcgg cttcgcagct
     9421 tcctttcaat ctcaaaacat tccggtgcct tgatgtcttc gagattgatg ccgccgaaag
     9481 taggttccag gctggcgata atgtccacca gcttatcggg gtcgcgctcg ttgatttcga
     9541 tgtcgaaaac gttgatgccg gagaatttct tgaagagaac tccttttcct tccatcaccg
     9601 gctttgccgc cagtggcccg atggggccga gccccagcac cgcagtgccg ttggtaatta
     9661 ccgccaccag gttgccgcga ccggtgtagt tgaataccgt gttcgggtcc tcggcgattt
     9721 cgaggctggg tacggccacg ccgggcgtat aagcgagcgc cagatcggcc tgggtggcaa
     9781 gcggcttggt ggcgatgact tcggtttttc caggagtggg gaagcggtgg tagtgcagcg
     9841 cgctttcggt cagcttgtca ggcattttta ttaagagaga aaaagtggtg ccggcaaaag
     9901 gattcgaacc ctcgaccaac tgattacaaa tcaggtgctc taccaactga gctatgccgg
     9961 cgtggaagtt gattatagaa cttcgagcgg accccagcaa tgaagatgaa acagaaccaa
    10021 ggccgcccgg ccgcgaaagc ctgctttatt tgacgacttg caggcgcggt ttgccgttag
    10081 gcgtggaagg agaaggatta tcttcgcccg tcttctgttc ggccgcagca ggcgcatcat
    10141 tgaagaacag gccctggccg ttttctttgg caaaaactcc gttcaccgca tccaggggaa
    10201 cggaaagctc tcgtgaaaca ccgtcaaaac gcgccgagaa ttgaatcagt tcatttccca
    10261 gcgtgagatt gcgcgtcgcg tcccggctga tgttgaggat gatttcgccg tttttgacgt
    10321 attccattgg cacgcgcgtg tgctcgttca ctttcaccgc aagataaggc gtcaatccgc
    10381 tgtcggcaca ccattcacag agcgcgcgga tcagataagg tttgctggaa atgttcttca
    10441 tttgttacca gcataggtca gaaatgcaag aagtgcaaaa caagtatttg gttctaagac
    10501 aaggcgcgag ctttgatcga agggcgccgc gacacggttg cgaagccggg tgcgggagcg
    10561 gcgccataaa ctctggttag cagtgccctc tgcggcgctc acatccctgt ctccgctctg
    10621 agcttcgccg ccgtgagcca gagtgcagtg tatatgagaa atacatgagc gagcaaagag
    10681 agcaacgtcg tatcgggacc cgccgttttg cctacttgcg catggcgcgt tcgggcgccg
    10741 tcagcgcgtc gatgaacgcg gggcggctga acaaacgctc ggcgtatttc ataagcagcg
    10801 ctgcaggctt gcccaattga atgccgtaat ggtcgagccg ccacaacagc ggcgcgattg
    10861 ccacatcgag catggaaaac tcatccccca gcatgtattt atgcttggcg aagaccggcg
    10921 cgatctgcgt aagattgtca cggatcacca agcgcacctt gtcggcggtt ttttgattgc
    10981 cgtgctcgat agtctcgatt tgcgagaaga gttcgagctc gaagcggaac aggaacaggc
    11041 gggcgcgggc acgcatcacc ggatcggccg gcatgagttg cggatgggga aaacggtcgt
    11101 cgatgtattc gttgatgatg ttggcttcgt acagaatcag gtcgcgctcc accagtaccg
    11161 gcacccggtt gtacgggttc atcaccgcca ggtcctcagg catgttgtag agatccacat
    11221 ccactatctg gaaatccatg cccttttcaa aaagcacgat gcggcagcgg tggctgaaaa
    11281 gacaggtggt tccggagtat aaggtcatca tggctgttac cgcccgctag agttgttatt
    11341 cacatggctc cccaaggacg gtctaatgaa cgtccttcca gaattccttc ttcaacgcgt
    11401 aggtgagcac gaacaggatg ccgaggaaaa tcagcacaaa catgcccagc tgcacgcgga
    11461 aattgcgcgc cggctcggcc atgtacacga gataatttac caggtccgcg accagcatgt
    11521 cgtattgctg cggcgtgagc acgcccggct tttccagtat cagcttgtga tcctcgtgct
    11581 ggtgtccctg tgaatcggtc ttttcttccg tcttcagcat ctgcacgccc tgcaattcgt
    11641 gcagcacgtg cggcataccc accagcggaa ataccgtgtt gttccagccg gtgtgcgtgc
    11701 tgtcgtcgcg gtaaaagctg cgcaaatagg tgtagagcca gtcggcgccg cgcacgcggg
    11761 caatcagcga caaatctggc ggcggcgcgc cgaaccagtc cttggcgtcg ctcttgtcca
    11821 tcgccacggt catggtatca cccacgcgtt tcgccaccgc gaacatcagg ttgtccttga
    11881 tttgctgctc ggtgaggccc aggtcctgca aacggttgta gcgcatgtaa gccgcattgt
    11941 ggcaggtcac gcagtagttg acgaaaatgc gcgcgccgcg ctgcagcgaa atcttgtcgt
    12001 gctggttgac cggtgcggtg tcgtacttcg catcgcttga agcgagggcc gcaaccggaa
    12061 caagcattaa agccagcagg agttttttca tcgctatctc accctttccg gttccggttt
    12121 ggtcttgtca aacctggtgt accagggcat cagcaggaag aacgcgaaat agatgagggt
    12181 gcagatttgc acaatgatag tgtacagtct gctcaccggt tgcgtgccca gaagacccag
    12241 caccaggaag ctaattacga acagcgccag cgccgtttta taaagcgcac cgcggtagcg
    12301 gatcgacttc accgggcttt ggtccagcca gggcagcaag atgaaaatca ttaccgatat
    12361 ccccatcagc gcgacgcccc agattttggc ttcaaacaca aacgtcgcca cgaaaatcag
    12421 tgcgccgatt atggcgatgg taaacttaaa gtgattctgt tttacgccga tgatcgccag
    12481 gatcatgtac acaaccaacc ctactctgag aaaccacata aaatcttccg tggtggcgcg
    12541 caaaatcgag tagaacgctg tgaagtacca cagcggcgcg atatgctccg gcgtctgcaa
    12601 cggattggcg gggatgaagt tgttggcttc caggaaatag ccacccatct ccggagcaaa
    12661 aaacacggca atcgagaaca caaacaggaa caccaccgcg ccgaccatgt cctttaccgt
    12721 gtaatacgga tggaaaggaa tgccgtcgag cggaatcccg gttttgggat ccttgttctt
    12781 cttgatttcg atgccgtcag ggttgttcga gcccacctcg tgcagcgcaa tcaaatgcgc
    12841 cgccaccagg cccagcagca ccagcggtac agcgattacg tgaaaggcga agaagcggtt
    12901 gagcgtcgcg tcggacaccg cataatcacc gcgtatccac agtgcaaggt cggggccgat
    12961 caagggaatc gcgtcgaaca gattcacgat cacctgcgcg ccccagaacg acatctgccc
    13021 ccaaggcagc agataaccga aaaacgcttc cgccatcagg catagataaa tcagcatgcc
    13081 gaaaatccag atgagctcgc gtggcttgcg gtaggagccg taaagcaggc cgcggaacat
    13141 gtgtaggtac accaccacga agaacatcga ggcgccagtg gaatgcatgt agcgtatcaa
    13201 ccagccccaa ggcacatcgc gcatgatgaa ttccaccgaa gcgaacgcga gactcgcgtc
    13261 cggtttatag ttcatggtga ggaagatgcc ggtgacaatt tgcagcacca gcatcaaaag
    13321 cgccagcgag ccgaagaaat accagaagtt gaaattcttt ggcgcgtaat attcggaaag
    13381 atgcgttttc cagctggcag tcaacggaaa acgctggtca atccagccga gcagcatggt
    13441 caattttttg gtcattgtca ggcccccttg ctgtcttcgc cgatgagaat cttgttatcg
    13501 ctcaaatagc ggtgcggggg aatcctcagg ttggtcggcg cgggcacgtt cttgaataca
    13561 cggcccgcaa ggtcaaagcg cgacccgtga cagggacaga aaaagccgcc cggccaatcg
    13621 gcgccgagat cgggcgcccc aacttccttg cggtaagtcg gcgagcagcc aagatgagtg
    13681 catagaccga tagcgatgaa gtattgcggt ttgagcgagc gcgtcaagtc tttgcagtag
    13741 cctggctgct cggaggattc ggaatccggg tcggcgagct tgtcattgag cttgggcagc
    13801 agctcgagca tttccttggt gcgattcaca atccacacca ctttgccttg ccactctaaa
    13861 tcgagcttgg cgcccggctc gattttgccg atatccactt ccaccggcgc gccgatggcg
    13921 agcgcccgct ggctcggcaa catagacacc acgaacggaa cggcagtggc ggctgtcgcc
    13981 acccctcccg ctaccgtggt ggcggcgata agaaagcgcc gtttgctgcg atccacctga
    14041 ttgttatcgg tcattcaacg cttccatgtc gtggcgacta aaaatccggt tattttacct
    14101 gaatcgaaaa ggaaacttaa atcccttgac gctaaaacag gatgagggag ccgacaaaaa
    14161 atactttgaa ttagagagtt acaacaactc ctgtcgaaat atttagtgaa tgtgcgcacg
    14221 caaaaacgca agtcgcctcc gggcacggcg aatgttaaaa tgcgaggcaa aaattgtttc
    14281 aggttaataa gttaattgac tctggccgca tttggcggct aaccagtcgc tagaacgaat
    14341 ccgcttacag cgggccgctc agttagtcgc gttaggcagc atcggcgcgg gcagcgcacc
    14401 gaagaaagaa accaacaatg gccaggtatg ttgaaagcaa cctcatcagg gacgaacaca
    14461 tagtctatga aaccaagctg cattggataa ccttcgtttc gctgaaggct ctgctgacgc
    14521 tcttcatcgc tccactcatt gctcgcgcca ccagcgagtt cgccatcacc aacaaacgca
    14581 tcatcatcaa ggtggggctc atctccaggc acacgcttga gatgaatctg tcgaaggttg
    14641 agtccgtagc tgtcgatcaa ggcatctgtg gacgaatcct caggtatggc tccattacca
    14701 tcatcggcac cggcggaacg aaagaactat tcgcctacct tgcgaatccg gtggaattca
    14761 ggaggcaatt tcaacagctg caagcctagt ggaagcctga atccgcatgc tgcgccggca
    14821 atcttctccg gcaaaaacaa agtttgctca agtactccct tttatgagaa agccttttgc
    14881 gcgataatga cgcgctcgac ttaacccgcg aacccagcaa atgcgcaaac tttggctgat
    14941 ctttgcccag accgcgaccg tctgcctggc ggtagtgttt gtggtcacca cgcttaaacc
    15001 ggaattgctg cccgccggct ggcgtggcgg caacgtggta tcgttcaggg aagcagcgcc
    15061 ggacgtcaca acgaaaaaaa tcaactccta cagcgacgcg gcgaaaaaag ccatgccctc
    15121 ggtggtcaac gtttttacca gcaaggaagt gcgcgtgccg cgtcacccgc tgatggatga
    15181 tccgttattc cggcatttct tcggcgaccg tggcgacgaa aagcgccgta g
//
LOCUS       DATIOW010000218         3253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_805860, whole genome shotgun sequence.
ACCESSION   DATIOW010000218 DATIOW010000000
VERSION     DATIOW010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805860"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..856
                     /locus_tag="VLL03_07300"
     CDS             248..856
                     /locus_tag="VLL03_07300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSS47201.1"
                     /translation="MSTDFSTSANLAVEYNAAEAAYCDLLAAISRRDESAFREFYEAT
                     LGKVYHLALRITGAPEAAEEVVPEVYLKVWREAHRYDALRGKVMTWLLIICRSRSLDY
                     LRRQEEAEPHPEPELLMTEQPAENGPDDLLSLLERDRAIHRALANLLPAQRQLIALAF
                     LKGYTHIEIARYTRLPLGTVKAHIRKALEKLKRSLDEGQTQR"
     gene            853..1335
                     /locus_tag="VLL03_07305"
     CDS             853..1335
                     /locus_tag="VLL03_07305"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSS47202.1"
                     /translation="MKKSFAQQTEEVLSHDIEEALLAAVTPQAPEPERAALLRQRVLD
                     KVKEAADNEPVSYSTVHAQEGPWISVAPLVDMKVLRQDGDSASFLLRVQPGGQLPDHV
                     HLEEEECLVLEGSLLLGEGIMLRAGDYHRAPKGSPHGPASTNTGALLFLRSDNPAYHP
                     "
     gene            1351..2349
                     /locus_tag="VLL03_07310"
     CDS             1351..2349
                     /locus_tag="VLL03_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HSS47203.1"
                     /translation="MATTVDNHRRVEKYHTPFWLPGSHLQTIYPAFLDWRYRVDYRRE
                     RWDTPDGDFIDLDWVDGKPENPLIVLFHGLEGGSRSHYALSLMHSVREFGWRGVVVLF
                     RGCSGEINRLPRAYHSGDSAEIDWILRRLKAHNPQSTIYALGVSLGGNALLKWLGEQG
                     EQAKNVIHAAAAVSAPLDLMAAGDRLGRGFNLVYTRIFMKSMKRKYLDKLKRFPNLFN
                     REAMLSSGTLREFDDVITAPVHGYKNTDDYWTRASSKPGLKNIRTPTLLINARNDPFL
                     PDHALPSPNEVSPAVTLEFSAQGGHAGFVSGAFPGHLDWLPQRIVSFFNNSEPQRR"
     gene            2432..>3253
                     /locus_tag="VLL03_07315"
     CDS             2432..>3253
                     /locus_tag="VLL03_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HSS47204.1"
                     /translation="MIPKEIFKAYDIRGIVGKTLTPEIVEAIGGAIGSEARAKTQKTI
                     AIGRDGRLSGSELSEALARGIQKAGVDVIDIGLCTTPMLYFATHHLQTHSGVMVTGSH
                     NPPDYNGLKIIIAGETLSGETIQKLRTRIEQDDLSKGKGNYAEKTITEDYIARIGSDI
                     KLARPMKIVVDCGNGVPGAFVPRLYRQLGCHVRELFCEVDGSFPNHHPDPSQPENLKD
                     VVQALKTTDAEIGLAFDGDGDRLGVVTKDGKIIYPDRQLMLFAADMLSRNPDAEVI"
BASE COUNT          743 a          939 c          914 g          657 t
ORIGIN      
        1 tcagcaagcc taccccgact attgtcacca ggcaggttaa aagcaggtgt cgcgacctgg
       61 tcatggactt aattttagaa tccctacaag gccttgcagg attttgcgga tcccgatctt
      121 tggggtctag ttacacataa ctactaccaa tattattacg caggaaatcg ttctttggat
      181 gtgctttttc gtctaaaaac ccgcacatcc atttgccgga aatcagcgta atgagcataa
      241 tgccagcatg agtacggatt ttagcacgtc cgcaaaccta gctgtcgagt acaacgccgc
      301 ggaggcggct tactgcgatt tgctggctgc gatttcacgg cgggatgaaa gcgcgtttcg
      361 tgaattttac gaagctacgt taggcaaggt gtatcacctg gcgctgcgca tcaccggcgc
      421 gccggaagcc gcggaagaag tggtgccgga agtgtatttg aaagtgtggc gcgaagcgca
      481 tcgctacgac gcgctgcgcg gcaaggtgat gacttggctt ttgatcatct gccgcagccg
      541 ctcgctcgac tatctgcgca ggcaggagga agcggagccg cacccggaac cggaattgct
      601 gatgacagag cagccagcgg agaacggacc ggatgatttg ctgtccttgc tcgagcgcga
      661 ccgcgcgatt caccgtgcgc tggcgaacct gctcccggcg cagcggcaat tgattgcgct
      721 ggcttttctc aagggctaca cgcacatcga gattgcgcgc tacacgcgtt tgcccctggg
      781 cacagtaaaa gcgcacatcc gcaaggcgct ggaaaaattg aaacgctcgc tcgacgaagg
      841 gcaaacgcaa agatgaagaa aagttttgcg cagcagacgg aagaagttct gtctcacgac
      901 atcgaagagg ccctgcttgc ggctgtgacg ccgcaagcgc ccgaaccgga gcgcgccgcg
      961 ctgctcaggc agcgtgtgct cgacaaggta aaagaagcgg ccgacaacga gccagtgtct
     1021 tattccaccg tccatgccca ggaaggcccc tggatttcgg ttgcgccgct ggtggacatg
     1081 aaggtgctgc ggcaggatgg cgattcggct tcgttcctgc tgcgcgtgca gcccggcggg
     1141 cagcttcccg accatgtgca tctggaagaa gaggaatgtc tggtgctcga aggctcgctg
     1201 ctcttgggcg aaggcatcat gctgcgcgcc ggcgactacc accgcgcacc caaaggctcg
     1261 ccgcacggcc ccgccagcac caataccggc gcgctgctgt tcttgcgcag cgacaatccc
     1321 gcatatcatc cataacccct gaactagcga atggccacga cggtggacaa ccatcgccgc
     1381 gttgagaaat atcatacgcc cttttggctt cccggcagcc atctgcaaac catctatccc
     1441 gccttcctcg attggcgtta tcgcgttgat taccggcgag agcgctggga tacgccggac
     1501 ggcgacttca tcgatctgga ttgggtggat gggaaaccgg aaaatccgct catcgtcctg
     1561 ttccacggtt tggaaggcgg ctcgcgcagc cattacgcgt tgagtctgat gcactccgtg
     1621 cgcgaatttg gttggcgtgg cgtggtggta ctgtttcgcg gctgcagcgg cgaaatcaac
     1681 cgcctgccgc gcgcctatca ttccggcgac agcgccgaaa tcgactggat tctgcgtaga
     1741 ttgaaagcgc ataatccgca aagcacaatt tatgcattgg gtgtttcgct cggcggcaac
     1801 gccctgctta aatggctggg cgagcaaggc gagcaagcca aaaacgtaat ccatgccgct
     1861 gcagcggtct ccgctcctct tgatttgatg gcggcgggcg atcgtctcgg ccgggggttc
     1921 aacctggttt acacccgcat tttcatgaaa agcatgaagc gtaaatatct cgacaaactc
     1981 aagcgttttc cgaacctttt caaccgcgag gcgatgctca gctccggaac attgcgggaa
     2041 ttcgatgatg tcattaccgc gccggtgcat ggctacaaga ataccgacga ttattggacc
     2101 cgcgccagca gcaagcccgg cctaaaaaac atccggacac ccaccctgct catcaacgcc
     2161 agaaacgacc cttttcttcc agatcatgca ttaccctcac ccaatgaggt ctcacctgcg
     2221 gtaacattgg agttttcagc gcaaggcggc cacgcggggt tcgtgagcgg cgcgtttccc
     2281 gggcatcttg actggttgcc gcagcggatt gtgagtttct ttaataactc tgaaccgcag
     2341 aggcgctgag gcgcagagaa agcggtttgt ctccgcgtct ttgcgtcccc gcagaaaata
     2401 aacggcaaat gttgtcgacc aaccgatagc aatgattcca aaagaaattt tcaaagccta
     2461 cgacatccgc ggcattgtcg gcaaaacgct gacgccggaa atcgtcgaag ccatcggcgg
     2521 ggctatcggc tccgaggcac gggcaaaaac acaaaaaacc atcgccatcg gccgcgacgg
     2581 ccgtctttcc ggctccgaat tatccgaggc gctggcaagg ggaatccaga aagccggtgt
     2641 cgatgtgata gacatcggac tatgcacaac accgatgctc tatttcgcca cccatcacct
     2701 gcaaacgcac tccggcgtca tggtgaccgg ttcgcacaac ccgcccgatt acaatggcct
     2761 caaaatcatc atcgccggcg agacgctctc cggcgaaacc attcaaaaac tcaggacacg
     2821 catcgagcaa gatgacttaa gcaaaggcaa aggtaattac gccgaaaaaa ccatcaccga
     2881 agattacatc gcccggatcg gcagcgacat caagctggcg cggccgatga aaatcgtggt
     2941 ggattgcggc aacggcgtgc ccggtgcgtt tgtgcccagg ctttatcgcc agcttggctg
     3001 ccatgtccgg gaattgtttt gtgaggtgga tggcagtttc cccaaccacc atcccgatcc
     3061 ctcgcagcct gaaaatctca aggatgtggt tcaggcgctc aaaaccaccg atgcagaaat
     3121 cggcctggcg tttgacggcg atggcgaccg gctgggcgta gtcaccaaag acgggaaaat
     3181 catctacccc gatcgccagt tgatgctgtt cgccgccgac atgctttcgc gcaatccgga
     3241 cgcggaggtt att
//
LOCUS       DATIOW010000219         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1719417, whole genome shotgun sequence.
ACCESSION   DATIOW010000219 DATIOW010000000
VERSION     DATIOW010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719417"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /gene="sufC"
                     /locus_tag="VLL03_07320"
     CDS             <1..664
                     /gene="sufC"
                     /locus_tag="VLL03_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HSS47205.1"
                     /translation="MGPNGSGKSTFAKTLAGHSDYNVTGGEVRYEGKNLLEMAPEERA
                     RLGLFLAFQYPIEIPGVSNSAFLRLAYNTMQAARGGEELDPLEFDDFVREKIKIVGMD
                     PSFLDRSVNEGFSGGEKKRNEIVQMAVLQPKLAVLDETDSGLDIDAVKIVANGVNRLA
                     RADNAIVLVTHYQRLLNYIVPDYVHVMEGGQIIKTGGKELALELETRGYDWVRTESEL
                     AA"
     gene            671..1984
                     /gene="sufD"
                     /locus_tag="VLL03_07325"
     CDS             671..1984
                     /gene="sufD"
                     /locus_tag="VLL03_07325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HSS47206.1"
                     /translation="MELVKTDRFIDSLLRGKPQASPSSWLNRIRAEALERANSLTLPT
                     TRDEEWRFTDPSPLMKFSFQPASVGTKLTQSDIQQRFVIPEASSDRLVFVDGVYAPAL
                     STHAGHGHGILISNLAQVGSAHGAVMEAHLARHARFENDVFCALNTAFLHDGAWVIIK
                     NNTNYPTPIHLLFIATQKETVAYPRCLVVAEQGSQCTLVEEYVSLNEEVHFTNAVTEI
                     VVGENAHITHTRLQNESRNSFHIAHCAATLVKDAYYACDTVTLGARFSRYNLYVVQAA
                     EGARCTLDGLALISGRQLADTHSFIDHVQPHGVSRQLHKCIVGGAAHAVFNGKIMVRP
                     GAQLIDSAQSSRNLLLSDKAHVDTKPQLEIFADDVKCAHGATVGQLDAEEVFYLRSRG
                     LSEAEARSLLTYAFAAEIIDKIPVASLARKLKQKVMEQTQVREQA"
     gene            1981..>2321
                     /locus_tag="VLL03_07330"
     CDS             1981..>2321
                     /locus_tag="VLL03_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSS47207.1"
                     /translation="MNKASTVRNEKSKTSALDVARVRADFPILARSINGKPLVYLDNA
                     ASSQMPQPVIDRLVRYQTQEHAYIHRAVHTLSERATAEYEEARRKAQRFINAGDEHEI
                     IFTRGTTEGINL"
BASE COUNT          540 a          664 c          677 g          440 t
ORIGIN      
        1 catggggccg aacggttcgg gcaaaagcac tttcgccaaa acgctggcgg gccattccga
       61 ctacaacgtg actggcggcg aagtgcgtta tgaaggaaaa aatcttctgg agatggcgcc
      121 ggaagagcgc gcccgcttgg gcctgtttct cgccttccag tatccgatag aaattcccgg
      181 cgtgagcaac agcgcttttc tgcggcttgc ctacaacacg atgcaggcgg cgcgcggtgg
      241 tgaagaactt gatccactgg agttcgatga tttcgtgcgt gagaaaatca agattgtggg
      301 aatggatccg agttttctcg accgaagcgt caatgaaggt ttttccggcg gtgagaaaaa
      361 acgcaacgaa atcgtgcaaa tggcggtgct gcagccgaag ctggcggtgc tggacgagac
      421 cgactcagga ctcgacatcg acgcggtgaa aatcgtggcg aacggcgtca accgcctcgc
      481 ccgcgcggac aatgcaattg tactggtcac gcactatcag cggctcctca actatatcgt
      541 gcccgactac gtgcatgtga tggaaggcgg gcaaatcatc aaaaccggtg gcaaggagct
      601 ggcgctggag ctcgaaaccc gcggctacga ctgggtgcgt accgaatctg aactcgcggc
      661 gtaagctgac atggaactcg ttaaaaccga tcgcttcatc gacagcttgc tgcgcggcaa
      721 gccgcaagcg agcccgtcaa gctggttgaa ccggatacgc gccgaagcgc tggagcgcgc
      781 caattcgctc acgctcccga ccacgcgcga cgaggaatgg cgcttcaccg atccttcgcc
      841 gctcatgaaa ttttccttcc agccggcgag cgttggcacc aagcttaccc agtccgacat
      901 ccagcagcgc tttgtcattc ccgaggcctc ttccgaccgg ctggtattcg tcgacggcgt
      961 ttacgcgccc gcgctttcca ctcatgccgg gcatgggcat gggatcctga taagtaatct
     1021 ggcgcaagtc ggatcagcgc acggcgcagt gatggaagcg catctggcgc gccatgcgcg
     1081 gttcgagaac gatgtctttt gcgcgctcaa caccgctttt ctccacgatg gcgcatgggt
     1141 cataatcaaa aataacacga actatcccac gccgattcat ctgctgttca tcgctacgca
     1201 aaaggaaacc gtcgcctatc cgcgctgcct ggtggtggca gaacagggca gccagtgcac
     1261 cttggttgaa gaatatgtaa gcctcaatga ggaagttcac ttcaccaatg cggtgacgga
     1321 gatcgtggtg ggtgaaaatg cacacattac ccacacacgg ctgcaaaacg aaagcagaaa
     1381 cagcttccac attgcgcatt gcgccgcgac gctggtaaaa gacgcgtatt acgcctgcga
     1441 cacggtgact ttgggtgcgc ggttttcgcg ttataacctg tatgtcgtgc aggcagccga
     1501 aggcgcgcgc tgcacactgg acggtctggc attgatttcc gggcggcagc tcgccgatac
     1561 gcacagtttt atcgatcatg tccagccgca cggcgtgagc cggcaattgc acaaatgcat
     1621 cgtcggcggc gcggcgcacg cggtgttcaa cggcaaaatc atggtgcggc cgggcgcgca
     1681 attgattgac tctgcgcaat cgagccgcaa cctgctgctc tcggacaagg cgcatgtgga
     1741 caccaagccg cagctggaaa tctttgccga cgacgtgaaa tgcgctcacg gcgcgacagt
     1801 gggacagctt gacgcggaag aagtttttta tctgcgcagc cgcggactga gcgaagcgga
     1861 agcgagaagc cttttgacct acgccttcgc cgccgaaatc atcgacaaga ttccggtcgc
     1921 ctccttggcg cggaagctta aacagaaggt aatggaacaa acccaggtga gagaacaggc
     1981 atgaataagg caagcacggt acgcaacgaa aaatccaaaa cttccgcgct ggatgtcgcg
     2041 cgcgttcgcg ccgattttcc gattctcgcc cgcagtatca acggcaagcc gttggtatat
     2101 ttggataatg ccgcaagcag ccagatgcct caaccggtga ttgaccggct ggtgcgatat
     2161 caaacccagg agcacgccta cattcaccgt gcggtgcaca ccttgagcga gcgcgccacc
     2221 gccgaatacg aggaagcgcg gcgcaaggcg cagcgcttta tcaacgccgg ggacgagcat
     2281 gaaatcattt tcacccgcgg caccaccgaa ggcatcaatc t
//
LOCUS       DATIOW010000220         2663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_296772, whole genome shotgun sequence.
ACCESSION   DATIOW010000220 DATIOW010000000
VERSION     DATIOW010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296772"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VLL03_07335"
     CDS             <1..410
                     /locus_tag="VLL03_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HSS47208.1"
                     /translation="GKNDSDEEIRNEAQWIAHELGVDVPLHFTAFHPDYKMMDVPPTP
                     PETLSRARAIAQQEGLQYVYTGNVFDTEGGTTYCTDCKAPLIVRDWHRILHYRVTQDG
                     RCPDCATPLAGEFEQFTRQFGRRRIPIALHAAR"
     gene            complement(425..1453)
                     /locus_tag="VLL03_07340"
     CDS             complement(425..1453)
                     /locus_tag="VLL03_07340"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HSS47209.1"
                     /translation="MYQRELETTAAAMVAKGKGILAIDESNPTCKKRFDSIGVESTAE
                     NRRAYRELLITVPGAAQFISGMILFDETIRQSAADGTPFPEALKRQGILAGIKVDTGA
                     KKLAGAPGEQVTEGLDGLRERFAEYRGMGASFAKWRAVINIGDVIPSQYCLDVNAHAL
                     ARYAALCQEAGLVPVVEPEVLMDGKHTLERCFEVTEATLRSVFNALYLQRVTLEHMVL
                     KASMVISGSECPKRAGVEDVAIQTLRCLKRTVPAAVPGIVFLSGGQGNEEATAHLNTM
                     NLMGPHPWPLSFSYGRALQGPALKTWKGQATNVAAAQKALYHREKMNSAACLGRYTAQ
                     MEMEKAAA"
     gene            complement(1510..>2663)
                     /gene="pyk"
                     /locus_tag="VLL03_07345"
     CDS             complement(1510..>2663)
                     /gene="pyk"
                     /locus_tag="VLL03_07345"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HSS47210.1"
                     /translation="TDSALGNQERVGLDYKELPRDVAPGAVLLLDDGRIVLEVKAVRG
                     NEIICNVRHGGVLSNNKGINRQGGGLTAPALTDKDKEDIKTAVQLKADYLAVSFPKSG
                     ADMHLARQLLRDAGGHALLIAKIERAEAIPALQEIMEACDGIMVARGDLAVEVGDAAV
                     PALQKRMIRVARGMNKVTITATQMMESMIENPIPTRAEVSDVANAVLDGTDAVMLSAE
                     TASGRYPVETVEAMSRICLEAEKSSEVELDRDFLNRVFTRIDQSIAMAALFTAYHLKV
                     KAIAALTQSGSTALWISRLNCGVPIYALTSETATRHRVSLFRDTYPLMVKYAGQDREE
                     LLREVERELLANDVVREGDLIVLTIGEPIGKSGGTNTMKIVKIGQHREA"
BASE COUNT          509 a          845 c          722 g          587 t
ORIGIN      
        1 cgggcaagaa tgatagcgat gaggaaatcc gcaatgaagc gcagtggatc gcacacgagc
       61 tgggagtgga tgtgccgctg catttcaccg cgttccatcc cgactacaag atgatggatg
      121 tgccgccgac cccgccggaa acattaagcc gtgcccgcgc cattgcccaa caagaaggat
      181 tgcaatacgt ctacaccggc aatgtcttcg atacggaagg cggcaccact tattgcacag
      241 actgcaaagc gccgctcatc gttcgcgact ggcaccgcat tttacattac cgcgtcactc
      301 aggatgggcg ctgccccgac tgcgccacgc cgcttgctgg cgaattcgag caattcaccc
      361 gccagttcgg ccggcggcgc atcccgattg ccctccacgc cgcccgctag cagtgggagt
      421 tgcttcaagc agcggctttt tccatctcca tttgcgcagt gtagcggcct aagcatgcag
      481 cgctgttcat tttttcacgg tgataaagcg ccttttgcgc tgccgctaca ttggtagcct
      541 gtcctttcca ggttttgagc gcgggcccct gcagcgcgcg gccgtaggaa aagctcaaag
      601 gccagggatg ggggcccatc aggttcatgg tgttcagatg cgcggtcgcc tcctcgttgc
      661 cctgaccgcc cgagaggaac acgatacccg gcaccgccgc aggcactgtc cgcttcaggc
      721 agcgcagggt ttgtattgcc acatcttcca cacccgcccg cttcgggcat tcgctccccg
      781 aaatcaccat gctggcctta agcaccatat gttccagcgt gacgcgctgc aggtagagcg
      841 cattgaacac gctgcgcagc gtggcttcgg tgacctcaaa acagcgctcc aaggtatgtt
      901 tgccatccat caatacttcc ggttccacca ccggcaccag ccccgcctcc tggcatagcg
      961 ctgcgtagcg cgccagcgca tgcgcattga cgtccaggca atactggctg gggatgacat
     1021 cgccaatgtt gatgacggca cgccatttgg caaagctcgc ccccatgcca cggtattctg
     1081 caaagcgctc gcgcaaacca tccaaacctt cggtgacctg ctctccgggc gcgccagcga
     1141 gcttcttggc gccggtgtcc accttgatgc cggcgaggat cccctggcgc ttgagtgctt
     1201 caggaaacgg cgtgccatcg gcagccgatt gccgtatcgt ctcgtcgaag agaatcatgc
     1261 cgctgatgaa ttgcgctgcc ccgggaacag tgataaggag ttcgcggtat gcgcggcggt
     1321 tttccgctgt ggattcgacg ccgatgctgt cgaaacgctt tttgcaggtg ggattgctct
     1381 cgtctatggc gagtatgcct ttgccttttg ccaccatcgc tgcggctgtg gtttcaagct
     1441 cacgttggta catatatgct cgtcctccaa taaaaatctt agcagtgaat aacaaaaagc
     1501 gctcgttttt cacgcctccc gatgctgccc tatttttacg atcttcatag tattagtgcc
     1561 tcctgacttg ccgatcggtt ccccgatggt cagcacgata agatcgccct cgcgtaccac
     1621 gtcgttcgcc agcagttccc tttccacttc gcgcaataac tcttcacggt cctggccggc
     1681 gtatttcacc atcagcgggt aggtatcgcg gaacaggctg acacggtggc gtgtcgcggt
     1741 ctccgaagta agcgcgtaaa tcggcacgcc gcaattgagc cgcgatatcc atagcgcggt
     1801 agagcccgat tgcgtgagcg ctgcgatagc cttgactttc agatggtagg cagtgaacag
     1861 cgcggccatg gcaatcgact ggtcaatgcg ggtaaagaca cgattcagga aatcgcggtc
     1921 cagctcaact tcagaggatt tttccgcttc caggcagatg cggctcatag cttcaaccgt
     1981 ctccaccgga tatctgccgc tcgccgtttc cgccgacagc atgactgcgt cggtaccgtc
     2041 cagcaccgcg ttcgccacat ccgacacttc ggcgcgggta ggaatcgggt tctctatcat
     2101 cgactccatc atctgcgtag cggtgatggt cactttgttc atgccccgcg cgacacgaat
     2161 catgcgtttt tgcaaagccg gcaccgcggc atcaccgacc tccaccgcaa gatcgccacg
     2221 cgctaccatg atgccgtcgc acgcttccat gatctcttgc aacgccggaa tcgcctcggc
     2281 cctttcgatc ttggcaatca acagcgcgtg gccaccggca tcgcgcagca actggcgggc
     2341 caagtgcata tcggccccgc tcttgggaaa cgatactgcc agataatccg ctttgagttg
     2401 aactgcggtc ttgatgtctt ccttgtcttt gtcggtgagc gcaggcgccg tgaggccgcc
     2461 gccttggcga ttgatgccct tgttgttcga caacaccccg ccgtgtctca cgttgcagat
     2521 gatctcattg ccgcgtaccg cctttacctc gagcacgatg cgcccgtcat ccagcagcag
     2581 cactgcgccg ggggcaacat ctcgcggtag ctccttgtaa tccagtccaa cccgctcttg
     2641 gttgcccagc gcactatcgg tgt
//
LOCUS       DATIOW010000221         4332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_2022930, whole genome shotgun sequence.
ACCESSION   DATIOW010000221 DATIOW010000000
VERSION     DATIOW010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2022930"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="VLL03_07350"
     CDS             complement(<1..981)
                     /locus_tag="VLL03_07350"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HSS47211.1"
                     /translation="MPKWQSDTSSQLRGEAYQDYILQGVSRTFALTIPQLPSGLRKPV
                     SNAYLLCRIADTIEDEPALSAGQKRYFSQQFIKVVAGEVPADTFAAEFYPLLSERTPE
                     AEKDLIRNTPQVISITHSFSGNQRVAMQRCVQIMANGMAMFQSNPSPYGLKDLLQLDS
                     YCYHVAGVVGEMLTELFCDYSEEIGKNRERMLELATSFGQGLQMTNILKDIWEDRSRG
                     ACWLPQDIFRECGFDLRNLSKDGHDSAFGAGLEKLIAIAHAHLRNALTYTLLIPKHET
                     GIRKFCFWALGMAVLTLRKISANRAFTSGNEVKISRRAVKATILATHLTVG"
     gene            1201..2325
                     /gene="hpnI"
                     /locus_tag="VLL03_07355"
     CDS             1201..2325
                     /gene="hpnI"
                     /locus_tag="VLL03_07355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HSS47212.1"
                     /translation="MTSAITLAALAYLVYALRCVIAFGSRQPKQSGYCPAVTLLKPVC
                     GMEPQLYQNLRSFCDQDYPDYQVVFGVRDAQDPALAIIERIIKEFPQHDLSLLVDETV
                     IGTNLKVSNLANMYRLAKHDLLVIADSDMRVDRNHLRAVAAPFEDTQVGAVTCLYKAT
                     PAAGLPSRLGAMFINDCFAPSVLVALSFQELRFCFGATMAVTRKALEASGGFPALADQ
                     LADDHMLGKHVAQQGLKVILPACMVENRVWEPSFRALFQHELRWARTIRAMQPAGYAF
                     YFITYPVAVSLLFLPLAPAAAGLTAAAVSVILRVLLHWAVHSSFRIPGSTAPWLAPIR
                     DLLGFLVWAASFFGRGVEWREQLFSVRSDGQLMNKGVRNP"
     gene            2322..3746
                     /gene="hpnJ"
                     /locus_tag="VLL03_07360"
     CDS             2322..3746
                     /gene="hpnJ"
                     /locus_tag="VLL03_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007583690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HSS47213.1"
                     /translation="MMKTLFLNPPSYEGFDGGAGSRYQARREIRSFWYPTWLAQPAAL
                     VPGSKLVDAPARGLSLNDILPLANEYELAVLHTSTPSFESDTKVAEALKAKNPKLTIG
                     FIGAHVAVSPEPSLLASRAIDFVARNEFDYTIHDIAEGRPLAEVDGISYRVNGKVTHN
                     RDRATIEDMDRLPWVVDVYQRDLVIEDYSIGYLQHPYVSLYTGRGCKSRCTFCLWPQT
                     IGGHHYRTRSPENVVAEIARAKEYFPQVKEFFFDDDTFTDDLPRAEEIAKGLGKLGVT
                     WSCNAKANVPYASLKVMKENGLRLLLVGFETGSQQILLNIKKGMHVDMARRFMVDCHK
                     LGVVVHGTFIMGLPGETHETIEETIRFAKEINPHTIQVSLAAAYPGTYLHKQAMENGW
                     LLRDSTHIVAQEGFQVSSLNYPHLPHEEIFAALERFYKRFYFRPSKIAEITFEMMQSW
                     EMTKRRLREGVEFFRFLYGREDRV"
     gene            3743..>4332
                     /gene="hpnK"
                     /locus_tag="VLL03_07365"
     CDS             3743..>4332
                     /gene="hpnK"
                     /locus_tag="VLL03_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis-associated protein HpnK"
                     /protein_id="HSS47214.1"
                     /translation="MKRLIVTADDFGASLPVNKAIEQAHQNGILTTASLMVGAAAAPD
                     AVERAQRMPSLKVGLHVVMVCGRPLLPAAQIPDLVDDQGNFSTQLARAGVNFFFRSKV
                     RRQLEAEIRAQFAAFRNTGLTLDHVNTHNHMHVHPTVLSLILKVGREYGVRAVRLPHE
                     PLLSSWRAARNSLPLRLAGALLLNPWMSLMKWRLKKA"
BASE COUNT          885 a         1274 c         1221 g          952 t
ORIGIN      
        1 gcctacggtt aaatgcgtcg ccagtatcgt cgccttcacc gcgcgccgtg agattttcac
       61 ttcattgccc gaagtaaaag cgcggttggc gctgatcttg cgcaaggtga gcaccgccat
      121 gcccagcgcc cagaagcaga acttgcgtat gccggtttca tgcttcggaa tgagcagggt
      181 gtaagtcagc gcattgcgca ggtgagcatg ggcgattgca atcagttttt ccagtcccgc
      241 tccaaaagcg gaatcgtggc catccttgga taaattccgc aaatcgaaac cgcattcacg
      301 gaaaatgtcc tgtggcagcc agcatgcgcc gcggctgcga tcttcccaaa tatctttcaa
      361 aatgttggtc atttgcaagc cctgaccaaa cgaagttgcc aattctaaca ttctttcgcg
      421 gttttttccg atttcctcag aataatcgca aaaaagttcg gtgagcattt ccccaaccac
      481 gccggcaacg tggtagcagt agctgtcaag ctgcagtagg tccttcaatc cgtaggggct
      541 cggatttgac tggaacatcg ccatgccatt ggccatgatt tggacacagc gctgcattgc
      601 tacgcgctga ttaccgctga agctgtgggt gatgctgatg acctgcggcg tgttcctgat
      661 caggtctttt tctgcttccg gcgtacgctc ggaaagtaat ggataaaact ctgcggcaaa
      721 cgtatccgcc ggcacttcgc cggcaacgac tttgatgaac tgctgcgaaa aatagcgctt
      781 ctgcccggcg ctcaaggcgg gttcgtcttc aatggtgtcg gcgatacggc acaacaggta
      841 agcgttgctc accggcttgc gcaggcccga tggcagctgg gggatggtga gcgcaaaagt
      901 gcgcgatact ccctgcagga tgtagtcctg ataggcttcg ccgcgaagct gactgctcgt
      961 atcggactgc catttcggca tgtttcttcc ctcgtgttcg tcaccttgcg ccgtggcgcg
     1021 agaggtttat gtcctgaagg cgcccatttt atcccaaatt acggcgccgg gccgccacgc
     1081 ccgcatcacc tgtttcccga aagaacgcgc gcgcgccgaa agccggatca atcatgctaa
     1141 aatccgcggc tggcaacaaa tattaatgca aggaacagca tcatcgctac gctcacctta
     1201 attacttccg cgatcacgct ggctgcgctc gcttatctcg tctatgccct gcgttgcgtc
     1261 atcgcctttg gcagccgtca accgaagcaa agcggatatt gccccgcggt caccctgcta
     1321 aagccggtct gcgggatgga accgcagctc tatcaaaatc tgcgctcgtt ctgcgaccag
     1381 gattatcccg attatcaggt ggtgttcggc gtgcgcgatg cacaggatcc cgcgctcgcg
     1441 atcattgaac gcatcatcaa agaatttcct cagcacgatt tgtcgctgct cgtggacgaa
     1501 acggtcatcg gcaccaacct caaggttagt aatcttgcaa acatgtaccg gttggcgaag
     1561 catgatctac tggtgatcgc cgacagcgac atgcgtgtgg accgcaacca tcttcgggcg
     1621 gtggccgcgc cgttcgagga tacgcaggtg ggtgcggtca cctgtttata caaggcaacg
     1681 ccggcggcgg gactgccatc gcggttgggc gcgatgttta tcaacgattg ttttgcgcct
     1741 tcggttttgg tcgccctgtc atttcaggaa ttgcgcttct gttttggcgc aaccatggcg
     1801 gtcacccgca aggcgctgga agcaagcggc ggatttcccg cgctcgccga ccagctcgcc
     1861 gacgaccaca tgctgggcaa gcatgtcgcg caacagggac tcaaagtgat attgcccgcc
     1921 tgcatggtgg aaaacagggt ctgggaacct agtttcaggg cgctgttcca acatgaactg
     1981 cgctgggcgc gaaccatacg cgcgatgcag ccggcaggtt acgcgtttta cttcattacc
     2041 tatccggtag cggtgtccct gctgtttttg ccgcttgcgc ctgcggcagc gggcttaacg
     2101 gctgcggccg tttcggttat actacgcgtt ttgctgcact gggcggtgca ttccagtttt
     2161 cgtatccccg gatccaccgc gccctggctt gcgcccatcc gcgacttgct tggcttcctg
     2221 gtgtgggcgg caagcttctt tggccgcggc gttgagtggc gggaacagct gttttcagtc
     2281 cgctccgacg ggcagttgat gaataaggga gtgcgtaatc catgatgaaa accctgtttc
     2341 tgaatcctcc gtcgtatgaa ggattcgatg gcggcgccgg ctcacgttac caggcgcggc
     2401 gggagatccg ctcgttctgg tatcccacct ggctcgccca gcccgcggca ctggtgcccg
     2461 gcagcaagct ggttgacgca cccgcgcgcg gcctgtcgct caacgatatt cttcctcttg
     2521 ccaacgaata cgagttggcc gtgctgcata ccagcacgcc ttcttttgaa tccgacacca
     2581 aagtcgctga agcgctgaaa gcgaaaaatc ccaagctgac gataggcttc atcggcgcgc
     2641 atgtcgcggt ctcccccgaa ccctcgctgc tcgcatcccg cgcgattgat ttcgtggcgc
     2701 gtaacgaatt cgactacacg attcatgata ttgccgaggg acgcccgctc gctgaagtcg
     2761 atggcatcag ctacagggtg aacggcaaag taacgcacaa ccgcgaccgc gccaccatcg
     2821 aggacatgga ccggctgccg tgggtggtgg atgtatacca gcgcgatttg gtgattgaag
     2881 attattctat cggttacttg cagcatcctt acgtgtcgct ttataccggg cgcggctgca
     2941 agtcgcgctg caccttctgt ctgtggccgc aaaccatagg cggccaccac taccgcaccc
     3001 gcagcccgga aaacgtggtc gcggaaattg cgcgcgccaa ggaatatttc ccgcaggtga
     3061 aagaattctt tttcgacgac gacactttca ccgatgattt gccgcgcgcc gaggagattg
     3121 ccaaggggct cggcaaactg ggcgtaacct ggtcgtgcaa cgccaaggcc aacgtgccct
     3181 atgcaagcct caaggtgatg aaggaaaacg gactgcgctt gctgctggtg gggttcgaga
     3241 ccggcagcca gcaaatcctg ctcaacatca agaagggcat gcacgtggac atggcgcgcc
     3301 gcttcatggt cgattgccac aagctcggtg tggtggttca cggcactttc atcatgggcc
     3361 tgcccggcga gacacacgaa accatcgaag agaccatccg cttcgccaaa gagatcaacc
     3421 cgcataccat tcaggtctcg ctcgccgcgg cctatccagg aacctatctg cacaaacagg
     3481 cgatggagaa cggttggctg ctgcgcgatt ccacgcatat cgtcgcgcag gagggcttcc
     3541 aggtcagctc gctcaattat ccgcacctcc cgcatgagga aatcttcgcg gcgctggaaa
     3601 gattctacaa gcgcttctac ttccgcccca gcaagattgc cgaaatcact ttcgagatga
     3661 tgcaaagctg ggaaatgacc aagcgccggc tgcgcgaggg cgtggagttc tttcgttttc
     3721 tttacgggcg ggaagatcgg gtttgaagcg gcttatcgtc acggctgacg acttcggcgc
     3781 atcccttccc gtcaacaagg caatagaaca ggcgcaccaa aacggcatcc tcaccaccgc
     3841 gagtctcatg gtgggcgctg ccgcagctcc agacgcagtg gaacgcgccc agcgcatgcc
     3901 ctcgctcaaa gtgggactgc acgtggttat ggtctgtggc cgcccgcttc tgcctgctgc
     3961 ccaaattccc gacctggtgg acgatcaggg aaatttctct acgcaacttg cgcgcgccgg
     4021 ggtcaatttc tttttccgct ccaaggtcag acggcaactc gaagcggaaa tccgcgcaca
     4081 gtttgcagct ttccgcaaca ccggccttac gctggatcac gtcaacaccc ataaccatat
     4141 gcacgtgcat ccgacagtat tgagcctgat actcaaggtg ggacgtgaat atggtgtgcg
     4201 cgcggtgcgt ctgccgcatg agccgttgtt gagttcctgg cgcgccgcgc gtaactcact
     4261 gccgctgcgt cttgccggtg cactgctgct caacccctgg atgagcctga tgaaatggcg
     4321 gctgaaaaaa gc
//
LOCUS       DATIOW010000222         4160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1922431, whole genome shotgun sequence.
ACCESSION   DATIOW010000222 DATIOW010000000
VERSION     DATIOW010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922431"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VLL03_07370"
     CDS             complement(<1..406)
                     /locus_tag="VLL03_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS47215.1"
                     /translation="MNKACIKCGYQRKAGDAAPETECPHCGAIYAKVEASLKAAPIQE
                     KERVEATGFLRAFREEIENWCQGRIWYGRALLLLWLAWIGVEHLRDPLYSSLFGGLNL
                     GIHEAGHLLFRFFGFEFLMVAGGTLLQLAAPVG"
     gene            complement(403..1800)
                     /gene="fumC"
                     /locus_tag="VLL03_07375"
     CDS             complement(403..1800)
                     /gene="fumC"
                     /locus_tag="VLL03_07375"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004532308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HSS47216.1"
                     /translation="MGKFRTERDTFGEIQVPAERLWGAQTQRSLENFRISGERMPPDF
                     IHALALVKRACAKVNRELGLLDAKKAEAIISAADEVIEGRRDEEFPLVVWQTGSGTQT
                     NMNMNEVLANRASELLGGERGEKRLVHPNDDVNKGQSSNDVFPTAMHITAAQALVNRL
                     IPCIQKLRGTLNRKAHAFEAIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLAHVEAV
                     LPHLYELALGGTAVGTGLNAHPEFAVRVANELAQEADLPFVSALNKFEALASNDALVH
                     AHGALKTLAASLMKIANDIRWLASGPRSGLGEIKIPENEPGSSIMPGKVNPTQSEAMT
                     MLCCQVFGNDVAITIGGASGNFELNVYKPLIIHNFLQSVRLLADGALSFNDNCAAGIE
                     ANRERIAELLNNSLMLITALNPHIGYDKAAQIAKKAHQEGISLREAAIASGHVTAAQF
                     DQWVRPQDMVGKKLN"
     gene            complement(1890..>4160)
                     /locus_tag="VLL03_07380"
     CDS             complement(1890..>4160)
                     /locus_tag="VLL03_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HSS47217.1"
                     /translation="PRYTRAVLLNLFFLGAGFFWAAWLAQARLADALPAEWEGKDIRL
                     SGVVASLPQENERGMRFELDVEEIQTQQAEVPAHISLTWYKDRRQPEKPLPQLHPGER
                     WQLTVRLKRPHGNANPHGFDYEAWLLERNIRATGYVRESAGNALIAESVARPSYLVER
                     MREAVREHLRQKLPQEPYAGVLTALAIGDQQAIPQSQWKIFTRTGVNHLMSISGLHVT
                     MVASLVLGLAYLFWKLVPRLALWLPARKAAVVAGALAALAYALLAGFEVPAQRTVYML
                     SVVAVALWFGRQSSASLVLCIALFVVTLLDPWAVLAPGFWLSFGAVAVIFFVTSGRVG
                     QSHWLAVWGRTQWAVTLGLTPLLLILFQQVSLISPLANAVAIPVVSFIVVPLTLLGSL
                     PPLDFLLPVAHLIMQWLMVLLEWMSALPDAVWQQHAPPAWAAVAGVAGVVWLLLTRGF
                     PARWLGCAGLLPLFLLAPAVPEPGELQLAILDVGQGLAVVARTQNHVLLYDTGPAFSS
                     ETDSGNRIIVPYLRAVGIKQLDGVIVTHADSDHSGGAISVLDSLPVDWLASSLPAEHP
                     IQTHARRSLTCYAGQAWEWDGVRFDILHPELSSYQNEKLKTNARGCVLKITSRYGAVL
                     LPTDIEKRSEEELLKRTPEQLQADVLIAPHHGSKTSSTEIFIEKVRPKTVIFTVGYRN
                     QFGHPKPEVVERYKTLGGKIYRSDLDGALTLDFDSRGVMVRSYRAERRRYWQSWPNAE
                     AGGAGDALSGDGGSSL"
BASE COUNT          859 a         1294 c         1181 g          826 t
ORIGIN      
        1 cgccgaccgg cgcggccagc tgcaacagcg tgccgcccgc aaccatcaga aactcgaatc
       61 caaaaaaacg gaacaacaga tggccggctt cgtgtatacc cagattcaag ccgccgaata
      121 ggctggaata aagcgggtcg cgcagatgtt caacacctat ccaggcgagc catagcaaaa
      181 gcagcgcgcg tccgtaccag atgcgccctt ggcaccagtt ttcaatttcc tcgcggaagg
      241 cacgcaggaa acctgttgct tctacccgtt ccttttcctg gattggtgcg gctttgagcg
      301 aggcttcaac cttggcgtaa atcgcgccgc agtgcgggca ttcggtttca ggcgcggcgt
      361 ctcccgcttt ccgctggtag ccgcatttga tgcaagcttt attcaattga gctttttacc
      421 caccatgtcc tgtggacgca cccattggtc gaattgcgcg gcggtgacat gacccgatgc
      481 gattgccgct tcgcgcaagc ttattccttc ctggtgcgct ttcttggcga tttgcgctgc
      541 tttgtcgtag ccgatgtgcg gattcaacgc ggtgatcagc atcagcgagt tattgagcag
      601 ttcagcaatg cgctcgcggt tcgcttcaat tcccgccgcg caattgtcgt tgaagctcaa
      661 agcgccgtcg gcaagcagcc gtacactttg caggaagttg tgaatgatga gtggtttgta
      721 cacgttgagc tcgaaattgc ccgacgcccc gccgatagtg atggcgacat cattcccgaa
      781 cacctggcag cagagcatgg tcatcgcttc ggattgggtc gggttcactt ttcccggcat
      841 gatggagctg cccggctcgt tctcgggaat cttgatttcg cccaagccgg aacgcgggcc
      901 gctcgccagc cagcggatgt cgttggcgat tttcatgagt gacgcggcga gtgttttcag
      961 cgcgccgtgc gcgtgcacca gcgcatcgtt gctcgccagc gcctcgaatt tattgagcgc
     1021 ggaaacaaaa ggcaagtcgg cttcctgtgc aagttcattc gcaacacgca ccgcgaactc
     1081 cggatgcgcg ttaagaccgg tgccgaccgc cgttccgccc agcgccaact cgtataaatg
     1141 cggcagcacc gcctcgacat gcgccaggcc atgatcgagc tgcgagacat agccggaaaa
     1201 ttcctggccg agcgtcaggg gcgtggcatc ctgcaaatgc gtgcggccga ttttgacgat
     1261 ggcctcgaac gcatgcgctt tccgatttaa tgtaccgcgc agtttctgaa tacacggtat
     1321 caagcgattg accagcgcct gcgctgccgt gatgtgcatc gcggtgggaa acacatcgtt
     1381 cgacgactgg cctttattca cgtcgtcatt gggatgcacc agccgctttt cgccgcgctc
     1441 gccgccgagc aattccgagg cgcggttcgc cagcacctcg ttcatgttca tgttggtctg
     1501 ggtgccggag ccggtctgcc acaccactag cggaaattcc tcgtcgcgcc ggccttcaat
     1561 cacttcatcc gctgcactaa taatggcttc ggcttttttt gcgtcgagca aaccgagctc
     1621 gcggttgacc ttggcgcagg cgcgcttcac cagcgccagc gcgtgaataa agtccggcgg
     1681 catgcgctcg ccggaaatcc tgaaattttc caggctgcgc tgcgtctgcg cgccccacag
     1741 cctctccgcc ggcacctgaa tttcgccgaa ggtgtcacgc tcggtgcgga acttgcccat
     1801 taccctctcc aaagaatgcg cctgatgaaa ttatagcttc tggcgcggtc cggttcgttt
     1861 aaaataatgg ccgaacagat aaatcaacct tataagctgc ttccgccatc gccggataag
     1921 gcgtcgcccg cgccacctgc ttcagcgttt ggccaggatt gccagtagcg gcggcgctcg
     1981 gcgcggtaag accgtaccat aacgccgcgc gaatcgaaat ccagcgtgag cgccccgtcc
     2041 aaatcggagc ggtaaatttt gccgccgagt gtcttataac gctccaccac ttcgggcttc
     2101 gggtggccga actggttgcg gtagccgacg gtgaaaatca cggttttagg tctgactttt
     2161 tcgataaaaa tttcagtcga agaagtcttg ctgccgtgat gcggcgcaat cagcacgtcc
     2221 gcttgcaatt gctccggcgt gcgtttgagc aactcctctt cagaacgttt ttcgatgtca
     2281 gtgggcagca gcacggcacc gtaacggctg gtgattttca gcacgcagcc gcgcgcattg
     2341 gttttgagtt tttcgttctg gtagcttgat aattcgggat gcagtatgtc gaaacgcacg
     2401 ccgtcccatt cccacgcttg cccggcgtaa caagtcagcg accgccgcgc atgagtttgg
     2461 attggatgct ccgccggaag agacgaagcg agccagtcca ccggcagact gtccagtacc
     2521 gaaatcgcgc cgccgctgtg atcgctgtcg gcgtgcgtga caatcacgcc atccagttgc
     2581 ttgattccca ccgcgcgcaa atacggcacg atgatgcgat tgccgctgtc ggtttccgag
     2641 gaaaaagccg gcccggtgtc gtagagcagc acgtggttct gtgttctcgc caccaccgcc
     2701 agtccctgcc ccacatccaa aatggcaagt tgtaattcac ccggctccgg cacggcaggc
     2761 gcgagcaaaa acagcggcag caagccggcg cagcccagcc agcgcgcggg aaaaccgcgc
     2821 gtcagcagca gccacacgac tccggccact cccgcgacag ccgcccacgc aggcggcgcg
     2881 tgttgctgcc aaaccgcgtc aggtaacgcg ctcatccatt ccagcaaaac catcagccac
     2941 tgcatgatga gatgcgccac aggcaaaagg aaatcgagcg gtggcaggct gcctaacaaa
     3001 gtcagcggca ccacgatgaa gctcaccacg ggaatggcga ccgcattggc gagcggcgaa
     3061 atcagcgaga cctgctggaa cagtatgagc agtagaggtg tgagtcccag cgtcaccgcc
     3121 cattgcgtgc gtccccagac ggcaagccaa tgcgattggc ccacgcgccc gctggtgacg
     3181 aaaaaaatca ccgcgaccgc gccgaaggaa agccagaatc ccggcgccag caccgcccag
     3241 gggtcgagca atgtcacgac aaacagcgcg atgcacagca ccagcgaagc ggaactctgt
     3301 ctgccgaacc acaacgccac cgccaccact gaaagcatgt acacggtgcg ttgcgccggc
     3361 acttcaaatc ccgccagcag cgcgtaagcc aacgcggcaa gcgcgccggc cacgaccgcc
     3421 gccttgcgtg ccggcaacca caaagcaagc cttgggacaa gtttccaaaa caagtaagcc
     3481 agacccagca ccaggcttgc gaccatggtc acatgcagcc cggaaatgct catcaggtga
     3541 tttacaccgg tgcgcgtgaa aattttccac tgcgattgcg gaatcgcctg ctggtcgccg
     3601 atggccagcg ccgtgagcac gccggcataa ggttcctgcg gcagtttttg ccgcaaatgc
     3661 tcgcgcaccg cttcccgcat gcgttccacc agataagagg ggcgcgcaac gctttcggca
     3721 atcagcgcat tcccggcgga ttcgcgcaca tagccggtgg cgcgaatgtt gcgctccaat
     3781 agccacgctt cgtaatcgaa accgtgcgga ttggcgttgc cgtgcgggcg cttcaagcgc
     3841 accgtcaact gccagcgctc gcccgggtgc agttgcggca gaggtttttc tggttgcctg
     3901 cggtccttgt accaggtcaa tgaaatatgc gccggcactt ccgcttgctg cgtctgtatt
     3961 tcttcgacat ccagctcgaa acgcatgccg cgttcatttt cctgcggcag gcttgccacc
     4021 acgccgctca agcggatgtc tttgccttcc cattcggcag gtaaggcatc ggcaagccgg
     4081 gcctgcgcca gccacgccgc ccagaaaaag ccggcgccga gaaaaaacag gttgagcaga
     4141 actgcgcgag tgtaacgggg
//
LOCUS       DATIOW010000223         2276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_197595, whole genome shotgun sequence.
ACCESSION   DATIOW010000223 DATIOW010000000
VERSION     DATIOW010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197595"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VLL03_07385"
     CDS             <1..554
                     /locus_tag="VLL03_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HSS47218.1"
                     /translation="SVTGHVNRPGNYEVPLGTPFTKLLELAGGMRGGRQLKACIPGGS
                     SMPVLPAHMMLATDMDYDSIARAGSMLGSGAVIIMDETVCMVKALLRLSYFYFEESCG
                     QCTPCREGTGWLYRVVNRIEQGHGRQEDLDLLNNVADNIAGRTICALGDAAALPVKSF
                     ITHFRDEFQHHIEHQQCLVPAYA"
     gene            562..>2276
                     /gene="nuoG"
                     /locus_tag="VLL03_07390"
     CDS             562..>2276
                     /gene="nuoG"
                     /locus_tag="VLL03_07390"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HSS47219.1"
                     /translation="MLQLEIDGRQIEVAEGSTVMDAANKLGIFIPYFCYHRKLSIAAN
                     CRMCLVQVEKAPKPLPACATPAMSGMKVFTHSEEAVKAQRGVMEFLLINHPLDCPICD
                     QGGECPLQDLAVGYGGSSSRYQEEKRVVADKNLGPLIATDMTRCIYCTRCVRFGQEIA
                     GVMELGMVGRGEHAEIMPFIEKTVDSELSGNMIDICPVGALTSKPFRYTARTWELSRR
                     KSVSPHDSLGSNLIVQVKENKVMRVLPRENEEINECWLSDRDRFSYEGLNSEERLTRP
                     MLKQDGEWKEVEWQTALEYIANKIKQIKNSHGAQAIGALVTPHSTLEELYLLQKLVRG
                     LGSGNVDFRLRQCDFSADAVSQGAPWLGMKIAEINRLDRVLVVGSMLRKDHPLLAHRL
                     RQAAKRSTRISLINAVDEALLMRIEHKAIVAPREMVKALAEVLKAAAKAKDQPGPLSA
                     QFAALNPSQEARRIAESLASGNNVAVFLGNMSQHHPQAAALHALAQELASLLGAKFGF
                     LGEAANSVGGYIAGAVPFRTPVGKHAKAMLENPLKAYLLLNTEVELDCHNPREAVAAM
                     LNAELV"
BASE COUNT          492 a          636 c          702 g          446 t
ORIGIN      
        1 tttccgtcac gggccatgtc aaccgtcccg gtaactacga agtgccgtta ggcaccccgt
       61 ttacgaagct tttggagctc gcgggcggca tgcgcggcgg gagacaactc aaagcctgca
      121 ttcccggcgg ctcctcgatg ccggtcttgc ccgcgcatat gatgctcgcc accgacatgg
      181 attacgattc gattgccagg gcgggctcga tgctgggttc cggcgcagtc atcatcatgg
      241 acgagacggt gtgcatggtg aaagcgctgc tccggctctc ctatttttac ttcgaggaat
      301 cctgcggtca gtgcacgccg tgccgcgaag gcaccggctg gctgtatcgc gtggtgaacc
      361 gcatcgagca aggccatggc aggcaggaag acctggatct gctgaacaat gtcgccgaca
      421 acattgcggg gcgcaccatt tgcgccttgg gcgatgccgc cgctctgccg gtgaaaagct
      481 ttatcaccca tttccgtgac gaattccagc accacattga gcaccaacaa tgtcttgtac
      541 ccgcttacgc ataggacagc catgctacag cttgaaattg acggcaggca gatcgaagtg
      601 gcggaaggca gcaccgttat ggatgcggca aacaagctgg gcattttcat tccgtatttc
      661 tgctatcaca ggaaactttc gattgccgcc aactgccgca tgtgtttggt gcaggtggag
      721 aaagcgccca aacctctgcc ggcctgtgcc acgccggcga tgagcggcat gaaggttttt
      781 actcattccg aggaggcggt aaaagcgcaa aggggcgtga tggaattttt gctgattaat
      841 catccgctcg actgcccgat ctgcgaccag ggcggtgaat gcccgctgca ggatctcgcg
      901 gtgggctacg ggggcagttc ctcgcgttat caggaagaaa agcgcgtggt ggcggacaag
      961 aatcttgggc cgctcattgc caccgacatg acgcgctgca tctattgcac gcgttgcgtg
     1021 cgcttcggcc aggaaatcgc cggcgtcatg gagctcggca tggtggggcg cggcgaacat
     1081 gcggaaatca tgcccttcat cgaaaaaacg gtggactcgg agctctcggg caacatgata
     1141 gatatctgcc cggtgggcgc gctgacttcc aagccgttcc gctacaccgc gcgtacctgg
     1201 gagttgtcgc gtagaaaatc agtgagcccg catgacagtt tgggctctaa tctcatcgtc
     1261 caggtgaagg aaaacaaagt catgcgcgtg ctgccgcgcg agaatgagga aatcaatgaa
     1321 tgctggcttt ccgacaggga ccgcttttcc tacgaggggc tgaattctga agagcgcctg
     1381 acccggccaa tgctgaagca agacggcgag tggaaagaag tggaatggca gactgcgctg
     1441 gaatacattg caaataaaat aaaacaaata aaaaattcgc atggcgcgca agcgataggc
     1501 gcgctcgtca ccccgcacag cacgctggaa gagctgtatc tgctgcaaaa gctggtgcgc
     1561 ggtttgggca gcggcaacgt ggatttcaga ttgcgccaat gcgatttcag cgccgacgcg
     1621 gtgtcgcagg gtgcgccgtg gctgggaatg aaaatcgctg agatcaaccg gcttgaccgc
     1681 gtgctggtag tgggcagcat gctgcgcaaa gatcatccgc ttctcgccca tcgcctgcgc
     1741 caggcggcga agcgcagcac gcgcatcagc ctcatcaacg cggtggatga ggctttgctg
     1801 atgaggattg aacacaaggc catcgtcgcg ccgcgggaaa tggtgaaggc gcttgcggaa
     1861 gtgctgaaag cggcggccaa agcaaaggac caacctggtc cattgagcgc gcaatttgcc
     1921 gcgctcaatc cctcccagga agcgcggcgc attgcggaga gcctcgcttc ggggaataac
     1981 gtcgcggtct ttctcggcaa catgtcgcaa catcacccgc aggcggccgc cttgcacgcg
     2041 ctggctcagg agctcgcttc gctcttgggc gcaaaattcg gtttcctcgg cgaagcggcg
     2101 aacagtgtcg gcggctatat cgcaggcgcg gtcccattta ggacgcctgt gggcaagcat
     2161 gccaaggcga tgctggaaaa tccgctcaaa gcctacctgc tgctcaatac cgaagttgag
     2221 ctcgattgcc acaatccgcg cgaggcggtt gccgcgatgc tcaacgccga gctggt
//
LOCUS       DATIOW010000224         9515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1518019, whole genome shotgun sequence.
ACCESSION   DATIOW010000224 DATIOW010000000
VERSION     DATIOW010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9515
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518019"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VLL03_07395"
     CDS             complement(<1..653)
                     /locus_tag="VLL03_07395"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HSS47220.1"
                     /translation="MKKAKKHLRIHQMTVGDFDRLFPHDDACKAYLFNHRWPEGVRCP
                     RCGTEKVYEVPFKAWHWQCKQCADDGYRFSVLVGTIFENTNKPLRDWFRVMHMMLTSK
                     KGISALQIYRVMGFGSYNTAHLMCNKIRVALGNVEFKKLVGYVEVDETFVGGKAKNKH
                     KGKGGRGDFGGTGRTSKTAVIGAVQRKGNVVARVIDNVDAITLGAFVREAVSHKVSLL
                     "
     gene            complement(1006..2454)
                     /locus_tag="VLL03_07400"
     CDS             complement(1006..2454)
                     /locus_tag="VLL03_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HSS47221.1"
                     /translation="MLARLEPHSANLSATALRKIAHILFVLPKVKTLPDVPAEETLRA
                     QFRRRGLKPKELAQTPLCATLPHGGLAVWVMLDASKPVFEQQTVLRKALQPLLEENPQ
                     TLCISVHGKGQQRAQAAERAVYTAWVNGAKLPSRKSKTKAKPLARIELHGCGNRNEFN
                     VLRAQAEGNLLCRELTVLPPNELTPGAYRKRIRKLARDCSWRHEEYDLRKLRRMGAGA
                     FVAVAQGSHEGDAAIVHLRYRHPQARQTLALVGKGICFDTGGHNLKPARYMQGMHEDM
                     NGSAVCLGILLAATYAKLPVNIECWLAIAQNHIGPKAYKQNEVVKALNGTTIEIVHTD
                     AEGRMVLADTLTLAAKAKPSLIIDFATLTGSMGVALGERYSGVLGNREEWLVRAVRAG
                     KASGERVCAFPLDEDYEQPLESKIADIKQCAMDSEADHILAARFLKRFVNDVPWLHMD
                     LSASSCKDGLGAVATDVTGFGVGWGLEMLRNL"
     gene            2535..3329
                     /locus_tag="VLL03_07405"
     CDS             2535..3329
                     /locus_tag="VLL03_07405"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HSS47222.1"
                     /translation="MRQYLNLMQHILEHGTRKNDRTGTGTLSVFGHQLRFDLNTGFPL
                     LTTKKIHLKSIIHELLWFLKGETNTKYLKENGVTIWDEWADENGNLGPVYGYQWRSWP
                     APDGGHIDQIAKIIEEIRRNPDSRRLLVSSWNVADLDKMALLPCHAFFQFYVAQGKLS
                     CQLYQRSADMFLGVPFNIASYSLLTLMVAQICELKPGEFVHTFGDAHLYLNHLRQARE
                     QLSRTPLALPQMKINPAVKNIFDFKYQDFTLEGYEPHPAIKAPIAV"
     gene            complement(3381..3911)
                     /locus_tag="VLL03_07410"
     CDS             complement(3381..3911)
                     /locus_tag="VLL03_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS47223.1"
                     /translation="MQKLPFKTSLLVIITAVSVIIFSLIGIAAITSHIPGVSSEIDEA
                     DAAVEPQSGKPFVSSVVPLDAKSEPTRYLVEAAKACPNCGVIESITANQEKGDAYAGH
                     KVEKNVNKKVTYQVKVRMDNGTYRVVSQQDQPVFRVGEKVKIVNGTVVQFEKTMMMDK
                     NLIFGLMLATLTSRVF"
     gene            complement(3936..4430)
                     /locus_tag="VLL03_07415"
     CDS             complement(3936..4430)
                     /locus_tag="VLL03_07415"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSS47224.1"
                     /translation="MSIRFCTTVFACVWLASVPAFADDEPAQINSNTEAFFKKYDTNH
                     DGFLTRRELKGRRDLLRVFDEADTDHDGKLDLDEYAAAEMAVHRAKAERFAEDSLITA
                     RVKAELLKDPDIKSLDVSVETYRGHVLLSGFVEDQEQVHKAERIASGVRGVVSVKNAL
                     IVKI"
     gene            complement(4653..6008)
                     /locus_tag="VLL03_07420"
     CDS             complement(4653..6008)
                     /locus_tag="VLL03_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSS47225.1"
                     /translation="MNRVRILLVDDDPGLLRLISLRLNASGYEVETAESGEQALARLS
                     VSAPQLVITDLRMGGMDGIALFDAIHKVSPTLPVIILTAHGSIPEAVDATKRGVFGFL
                     TKPFESKALLEQVEAALKLGGAMGVSSGEGDSVWRKDILTRSPVMENVLSQARLVAES
                     DASVLIHGDSGTGKELLAQAIHRASARNGKPFVAINCGAIPENLLESELFGYSKGSFT
                     GATQDRKGLFQAAHGGTLFLDEIGDMPLALQVKLLRVLQEKQLRPVGSTQSMPVDVRI
                     ISATHRKLEEEMAAGKFREDLFYRLNVVSLRIPALSSRREDIPLLAGQFLQNLAGKYN
                     KQISNFAPDALQLLLGASWPGNVRQLLNVVEQAVALCTTRIVPLTLVQKALQDEESHL
                     SSLDEAKKRFEREYLSQLLKITEGNVSQAARLAKRNRTEFYKLLKRHALDPALFKNPH
                     I"
     gene            complement(6005..6547)
                     /locus_tag="VLL03_07425"
     CDS             complement(6005..6547)
                     /locus_tag="VLL03_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS47226.1"
                     /translation="MTTKRSFQGIATLFLCGMLLSACSLVKEGLERPVYTASDADAYL
                     AYFRYASNLSNDDLKKEYTTVSQIYGKNKSDGSRLQLALLLSMPSASFRDDARALGLL
                     KEVVANKANDNTQLKDFALLLSAHIAEHKKQEDKIKGEQGRAEELGQKLEEMEQKLEA
                     LKGIERSIIERQQTQPAKIK"
     gene            complement(6544..7983)
                     /locus_tag="VLL03_07430"
     CDS             complement(6544..7983)
                     /locus_tag="VLL03_07430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS47227.1"
                     /translation="MRLYYPKSFLQLILLGFVLVSVPLVFAFFNAALYVDRLANQSQK
                     AVYQAVQATQGSRILVEQLTQLERTVRQFIILGDQSLLEGYAKTHQNFRETAFELARL
                     PLDEAQKKALDELVEREQKLYAGLQKNPQDPGTSKSMALEYVLLSDLAQSILAQSNLL
                     IDREVDVLQQTAAQAQRILLWQVLASIPIAILLAVGFTILIARPIHQLDNAIRQLGGE
                     HFSSTIEVKGPQDLENLGQRLDWLRQRLIELEQQKTRFLRHVSHELKTPLTAIREGSE
                     LLNDEVAGKLSGEQREITSILRQNSLQLQKLIEDLLSYHTSKFRNTALDLKAVDLKNI
                     VDKVTEDQKLALVSKNLQLQTHCCETTFLGDQEKLRVIVDNLLSNAIKYSPQGGTIQI
                     NLERDDKTLQLDVIDCGPGIAEEDKGRVFDEFYQGRPAANSAVKGSGLGLAITREHVV
                     AHGGNIEIMDGNASGAHFRVTLPCRRAGE"
     gene            8052..8588
                     /locus_tag="VLL03_07435"
     CDS             8052..8588
                     /locus_tag="VLL03_07435"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HSS47228.1"
                     /translation="MAKPSAALLTPSAPHLTKRHISIIAAMAKNRVIGRDNRIPWRLP
                     RELQMFKSLTMSHHIVMGRKTYESLNRLLPGRTTVIVTHDRKYRVPGAIVVDSLQAAI
                     AACGNDEEIFIIGGAQLFKEALPLANRIYLTMVQAEIEGDTTMPDFDLRGWKQISKEE
                     FKADEKNPYDYIFSVHHK"
     gene            complement(8636..>9515)
                     /locus_tag="VLL03_07440"
     CDS             complement(8636..>9515)
                     /locus_tag="VLL03_07440"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HSS47229.1"
                     /translation="ILLGLGVAAGGIYYSNPMKSRQYIEYAVAEGVEWYVIDSVEELR
                     KIVSVKQDAKLYLRIDTPNIGSDWPLSGKFGAHPTEVREIIRAAGELKADLAGVTFHV
                     GSQCRNPQNWRVGIENALKVFQQMRVAGLNPRLLNIGGGYPVRHVKPIPSIEKISEVV
                     NEAIARVPETIRIMAEPGRYLVSDAAYFVCRVVGTATRAGKRWMYWDAGVFGGIIETQ
                     EGLIYEVRTDRNGRFVPWVVAGPTCDSMDVLMRDHLLPEDVQEGDYIYIPNAGAYTSS
                     YASNFNGFPLPDVIVI"
BASE COUNT         1971 a         2833 c         2553 g         2158 t
ORIGIN      
        1 agcaagctga ctttatgcga gactgcctcg cgcacgaacg caccgagcgt aatggcgtcc
       61 acgttgtcaa tgacgcgggc aaccacattg cctttacgtt gaactgcgcc gattacggca
      121 gtcttgctcg ttctacctgt accgccgaag tcgccgcgac cgcctttccc cttgtgcttg
      181 ttcttggcct tcccgccgac gaaggtttcg tccacttcga cgtagccgac gagtttcttg
      241 aattcgacgt tgccgagcgc cacgcgaatc ttgttgcaca tcaggtgcgc ggtattgtag
      301 gaaccgaagc ccatgacacg gtagatttgc agggcgctga tgcctttctt gctggtcaac
      361 atcatgtgca tgacgcggaa ccagtcgcgc agtggtttgt tggtgttctc gaagatggtg
      421 ccgaccagca cggaaaaacg atagccgtca tccgcacatt gcttgcattg ccagtgccac
      481 gccttaaaag gcacttcata aaccttttcc gttccgcagc gcgggcagcg cacgccttcc
      541 ggccagcgat ggttgaaaag gtacgctttg caagcgtcat catgagggaa aagacggtcg
      601 aaatcgccta ccgtcatttg gtggattcgc aggtgttttt tggccttttt catagttgtc
      661 tccggttacg atggagccat taaaccatga aaaaagactg gtgtcaagta ctgcgtaatc
      721 gccctaatta agtcctctac ggattgcgtt gccggcaaaa tgcggatgga tgcaaggcgc
      781 gcgacgaagg tcgtagctgg gactacgacg gaggacccgc ctagcgttgc atctgcgaag
      841 ctgtgcaagc ggggcgggcc agcgctcgca aagcgcagag cagggcccca tggggatgtg
      901 accgcggcgc gagcgctctt ggcctgcctt cactgcgcac gttccgaggc gcgacgttac
      961 ggcaagcctt ccggaaagcc gccgtcgcaa ctgtgcagga cttaatcaga ggttccttag
     1021 catttccaac ccccagccga ccccaaagcc ggtgacatcg gttgccaccg cgcctaagcc
     1081 gtctttgcaa ctgctcgccg acaaatccat gtgcagccac ggcacgtcat tcacgaagcg
     1141 cttgaggaag cgcgcggcga gtatatggtc ggcttcgctg tccatggcgc attgcttgat
     1201 atcggcgatt ttgctctcca gcggctgctc gtaatcctca tccagcggaa acgcgcacac
     1261 ccgctcgccg ctcgccttgc ccgcccgcac tgctcgcacc agccattcct cgcggttgcc
     1321 aagcacgccg gaatagcgct cgcccaaagc gacgcccatg ctgccggtaa gagtggcgaa
     1381 atcaataatc agtgagggct tggcttttgc cgccagcgtc aacgtatcgg ccagcaccat
     1441 gcgcccctcg gcgtcggtat gcacgatttc gatggtggtg ccgttcagtg ccttcaccac
     1501 ttcgttttgc ttgtaagcct tgggcccgat gtggttttgc gcaatcgcca gccagcactc
     1561 gatgttcacc ggcagcttgg cgtaagttgc ggcgagcaaa atgccgaggc agaccgctga
     1621 gccgttcatg tcctcatgca tcccctgcat gtagcgcgcg ggtttcagat tatgtccgcc
     1681 ggtgtcaaaa cagattccct tgccgaccag cgccagcgtt tgtcttgctt gcggatggcg
     1741 gtaacgcaaa tgcacaatag cggcgtcccc ctcgtgactg ccctgcgcca cggcgacaaa
     1801 cgcgccggca cccatcctgc gcaatttcct gagatcgtat tcctcatgcc gccaactgca
     1861 gtccctggcg agttttctga tgcgcttgcg gtacgcgccg ggcgtcagtt cgttgggcgg
     1921 gagtacggta agttcgcggc acaagagatt accttccgct tgcgcccgaa gtacattgaa
     1981 ttcatttcga ttcccgcatc catggagttc gattctggcg agcggcttag ccttggtttt
     2041 gctttttctg ctgggaagct tcgcgccatt tacccatgcc gtgtaaaccg cccgttcagc
     2101 cgcctgcgcc cgctgctgcc ctttgccatg caccgagatg caaagcgttt gcgggttctc
     2161 ttccaggagc ggctgcagcg ctttgcgcag cacagtctgc tgttcgaata ccggtttgga
     2221 agcatcgagc atgacccaaa ccgccagccc gccgtgcggg agagtggcgc acaaaggcgt
     2281 ctgcgccagt tccttcggct tcaagccacg gcgcctgaac tgggcgcgca aagtttcttc
     2341 agccggcaca tccggaagcg ttttcacctt aggcagaaca aacaagatat gcgctatttt
     2401 ccgcaatgct gttgcggaaa ggtttgcgga atgcggctcg agcctcgcca gcatgaaaag
     2461 tccttggtta gggtaaaatg atgaggaact tgagattata accaagctac ccgcttacga
     2521 acatcggcgc tgatatgcgg cagtatttga acctgatgca gcacatcctc gagcatggga
     2581 cgaggaaaaa cgaccgcacc ggcaccggca ccttgagcgt tttcggccat cagctgcgct
     2641 ttgatttgaa tacaggtttc ccgcttctca ccactaaaaa aatccatctc aaatccatca
     2701 tccatgagct gctgtggttt ctcaaaggcg agaccaatac caaatatctg aaagaaaacg
     2761 gcgttaccat ctgggatgag tgggcggatg aaaacggcaa tctcggcccg gtgtacggct
     2821 accaatggcg ctcctggccc gcgcccgatg gagggcacat cgaccagatt gcgaagatca
     2881 tcgaggaaat aaggagaaat ccggattcac gccggctctt agtttcgtcc tggaacgtcg
     2941 ccgacctcga caagatggcg ctgctaccct gccatgcttt tttccagttt tacgtggcgc
     3001 aaggcaaact ttcctgccag ctttaccaga ggagcgccga tatgtttctc ggcgtgccgt
     3061 tcaacattgc ttcctattcg ctgctcacgc ttatggtagc gcagatatgc gaattgaagc
     3121 ccggcgaatt tgtacatacc ttcggcgacg cgcatcttta tctcaaccat ctcagacagg
     3181 cgcgcgagca attatcgcgc acaccgcttg cgttgcctca gatgaaaatc aatcctgcgg
     3241 tcaaaaatat attcgacttt aaataccagg atttcactct agaaggctat gagcctcatc
     3301 ccgcgatcaa ggcgccgatt gcggtctaac cccaaaatga caagaggcgg aatcgcttcc
     3361 gccccttagg cctcggtcct tcaaaaaacc cgacttgtta acgtagccag catcagccca
     3421 aatatcaggt ttttgtccat catcattgtc ttttcgaatt gaactactgt gccgttgacg
     3481 attttgactt tctcgccgac acggaaaacg ggttggtctt gctgcgaaac gacgcggtaa
     3541 gtgccattat ccatgcgcac cttgacctgg taagtgactt tcttgttcac gttcttctcg
     3601 actttgtggc cggcataggc gtcgcctttt tcttgattcg ccgtgataga ttcgatcacc
     3661 ccgcagtttg gacaggcttt cgccgcttcc accaggtacc gcgtcggctc acttttcgcg
     3721 tctaacggca ccacgcttga gacaaagggc ttgccgcttt gcggttcgac cgccgcatcc
     3781 gcttcatcga tttcggaact gacaccgggg atatggctgg taatcgcggc aattccgata
     3841 aggctgaaaa tgatcaccga aaccgccgtg atgataacca atagactggt tttgaatggc
     3901 agtttctgca cggtattccc tccttaacgg tttatctaaa tctttacaat cagggcgttc
     3961 ttcaccgata ccacgccgcg cacaccggac gcgatgcgtt ccgccttgtg cacctgctcc
     4021 tgatcctcga caaaaccgga gagcagcaca tggccgcggt aggtctccac gctcacgtcg
     4081 agcgacttga tgtcggggtc cttcaacagc tcggccttaa cgcgcgcggt gatcaggctg
     4141 tcttcggcga agcgttccgc tttggcgcga tgcaccgcca tttccgccgc ggcgtactca
     4201 tccagatcaa gcttgccgtc atgatcggtg tccgcttcgt caaatacccg caacaggtca
     4261 cggcgccctt tcagttcccg ccgggtgaga aagccgtcgt gattggtgtc gtactttttg
     4321 aaaaacgctt cggtattgga attgatttgc gccggctcat cgtccgcaaa cgccggcacg
     4381 ctcgccagcc acacgcaggc gaacacggtt gtgcaaaaac gaatgctcat aatctgcctc
     4441 cattgcttcc atcggatcga cgcgagcgcc gccaacctta gctgtgtatc agcagtcctc
     4501 gtgccaaaat aaaaatcctt taaaaacagc tttatttgct taaaacagtc gacaaaaatg
     4561 tcgccgggtg acgacggccc atcggatgcg gtatttatgt gtttgattga tgaaggcttc
     4621 tgctgtcgcc aaacgacgac agcgcgaatc ttttagatgt gagggttttt gaacagtgcg
     4681 ggatcgagcg cgtggcgttt taacagcttg taaaactcgg tgcggttgcg cttggccagc
     4741 cgcgccgcct gagaaacatt gccttcggtg attttcagca attgcgaaag atattcgcgc
     4801 tcgaagcgtt tcttcgcttc atcgagcgag gatagatggc tttcttcatc ctgcagcgct
     4861 ttttgcacca gggtgagcgg cacaatgcgg gtcgtgcaca aagccaccgc ctgctccacc
     4921 acgttgagca attgccgcac attgccgggc caggacgcac ccagcagaag ctgcagtgca
     4981 tccggagcaa agttgcttat ctgcttgttg tacttgcctg ccaggttttg caaaaactgc
     5041 ccggcgagca gaggaatatc ttcacggcgg gaagacaatg ccggaatcct gagcgacacc
     5101 acgttcaggc ggtaaaacaa atcctcgcgg aatttccccg ccgccatttc ctcttcgagt
     5161 ttgcggtgcg tggcggaaat gatgcgcaca tccaccggca tgctttgcgt cgagcccacc
     5221 ggccgcaatt gcttctcctg caacacgcgc aggagcttta cctgcagcgc gagcggcatg
     5281 tcgccgattt catcgaggaa cagtgtgccg ccgtgcgccg cctggaacag gccttttcgg
     5341 tcctgcgtcg cgccggtgaa cgaacccttg ctgtaaccga aaagctcgga ctccagcaaa
     5401 ttctctggaa tcgcaccgca gttgatggcg acaaacggtt tgccgttgcg ggcactcgcg
     5461 cggtgaatcg cctgcgccag cagctccttg ccggtgccgg aatcaccgtg aatcaggacg
     5521 ctggcgtcgc tctccgccac cagccgcgct tgcgacagca cgttttccat caccggacta
     5581 cgggtgagaa tatccttgcg ccagacgctg tcaccctcgc cgctgctcac ccccattgca
     5641 ccacccagct tcaacgccgc ttcgacttgc tcaagcagcg ccttgctctc aaacggcttg
     5701 gtgagaaagc cgaacacccc gcgcttggtg gcatccaccg cttcgggaat cgagccatgt
     5761 gcggtgagaa taatcaccgg cagcgtcggg ctcaccttgt gaatcgcatc gaaaagcgcg
     5821 atgccgtcca ttccacccat acgcaaatcg gtaatcacca gttgcggcgc ggaaacggaa
     5881 agccgggcca gcgcctgctc gccgctttcc gcggtttcca cctcgtaacc cgaagcgttc
     5941 agccgcagcg atatcaggcg cagcaggccg gggtcgtcgt ccaccaaaag tatccgcacc
     6001 ctattcattt tatcttcgcc ggttgggttt gctgcctctc gatgatgctg cgctcaatgc
     6061 ctttcagcgc ctcgagcttc tgctccattt cctcgagttt ctgccccagt tcctcagcgc
     6121 gtccctgttc gcccttgatt ttgtcttcct gcttcttgtg ctcggcaata tgagcggaga
     6181 gcagcagcgc aaaatccttg agctgagtat tgtcattggc tttgttggcg accacttcct
     6241 tgagaagccc cagcgcccgc gcatcgtcgc gaaagctcgc ggagggcata gaaagcagca
     6301 gagccagttg caaacgactg ccgtcgcttt tgttcttgcc gtaaatctgg ctcaccgtcg
     6361 tgtattcctt cttgaggtca tcattggaaa ggttggaagc atagcggaaa taagcgagat
     6421 aagcgtccgc atcgctggcg gtatagaccg ggcgttcaag accctccttg accaaagagc
     6481 aggcactcaa cagcatgccg cacaaaaaca gcgtggcaat tccctgaaag gaacgcttcg
     6541 ttgtcattcg cccgcccgcc tgcacggcaa ggtcacgcgg aaatgcgcgc ctgatgcgtt
     6601 gccgtccatg atctcgatat tgccgccatg cgccaccaca tgctctcttg ttattgccag
     6661 gccgaggccg ctgcccttga ccgcgctgtt ggccgccggc cggccctgat aaaactcatc
     6721 gaacacccgg cctttgtctt cctcggcaat gcccgggccg cagtcaatca catcaagctg
     6781 cagcgttttg tcgtcgcgct ccaggttgat ctgtatcgtt ccgccttgcg gagagtattt
     6841 gatggcattc gatagcaaat tatcaacgat tactcgcagc ttttcctgat cccccaaaaa
     6901 agtggtttcg cagcaatgcg tttgcagttg caggtttttc gacaccagcg ccagcttctg
     6961 gtcttctgtc actttatcca cgatgttttt gaggtcgacc gccttcaggt cgagagcggt
     7021 gttgcggaac ttgctggtat ggtagctcag caaatcttca atcagtttct gtaattgtag
     7081 gctgttttgg cgcaggatgg aggtgatttc ccgctgctct ccgctcaatt tccccgccac
     7141 ttcatcgttc aacagttcgc tgccttcgcg gatcgcggta agcggcgttt tcagttcgtg
     7201 cgatacatgc ctcaagaaac gggttttctg ctgctcgagt tcaatcagcc gctgccgcag
     7261 ccagtcgagc cgctgcccga ggttctccag atcctgcggc cctttcactt cgatggtcga
     7321 ggaaaaatgc tcgcccccca gctggcggat cgcgttgtcg agctggtgga tcggccgcgc
     7381 gataagaata gtgaaaccca ccgccagcag gatggcaatc gggatggacg ccagcacctg
     7441 ccacagcaga atccgttgcg cctgtgccgc ggtctgttgc aacacatcca cttcgcggtc
     7501 aatcagcagg ttgctttgcg ccagaatgga ttgggcaaga tcggagagca atacgtactc
     7561 cagcgccatg gacttgctgg tgccggggtc ttgcggattt ttttgcaagc cggcatagag
     7621 cttttgctcc cgctccacca gctcgtccag cgcttttttc tgcgcctcgt cgagcggcag
     7681 tcgcgcaagt tcaaatgcgg tctcgcggaa gttctggtgc gtcttggcgt agccctcgag
     7741 caggctctgg tcgccgagaa tgatgaactg gcgtaccgta cgttccaatt gcgtgagctg
     7801 ctcgaccagg atgcggctgc cctgggtcgc ctgcaccgcc tggtacaccg ctttttggct
     7861 ctggttggcg agccgatcaa cgtacagtgc agcgttgaaa aaagcgaaaa ccagcggcac
     7921 ggacaccagg acaaagccca gcagaatcag ctgcagaaat gatttggggt agtagagcct
     7981 cactgcggga accgccagcg tgatttataa ttcgcgtaaa atctccattc aggcaataca
     8041 ataacataac cgtggccaaa ccctctgcgg cattgctcac accctcggca ccccatttga
     8101 caaaacggca tatttcaatc atcgccgcga tggcgaaaaa ccgcgtgatc ggcagggata
     8161 accgcattcc ctggcgcctg ccgcgcgaat tgcagatgtt caaatcactc accatgagcc
     8221 accacatcgt catggggcgc aagacttacg aatcgctgaa tcggcttttg cccggccgca
     8281 ccacggtgat tgtgacgcac gaccgcaaat accgggtgcc cggcgccatc gtggtggatt
     8341 cattgcaggc ggcgattgcg gcttgcggca acgatgagga aattttcatc atcggcggcg
     8401 cgcagttgtt taaagaggcg ctgcctcttg ccaaccggat ttatctcacc atggtgcagg
     8461 cggaaatcga aggcgacact accatgcccg atttcgactt aaggggctgg aaacaaatat
     8521 cgaaagaaga atttaaagcc gacgaaaaaa acccgtacga ttacatcttt tcagtgcacc
     8581 acaaataaaa agcggattgc ggcccgtgcc gcaatccgct ttgtcgcatt aaaaactaga
     8641 taacgatcac atccggcagc gggaaaccgt tgaagttgct ggcgtaggag ctggtgtatg
     8701 cgccggcgtt gggaatgtag atgtaatcgc cttcctgcac gtcttccggc aacaagtgat
     8761 cacgcatcag cacgtccatc gaatcgcagg tcgggcccgc caccacccag ggaacgaacc
     8821 ggccgttgcg gtcggtgcgc acttcataaa tcagaccttc ctgggtttcg atgatgccgc
     8881 cgaacacgcc ggcgtcccag tacatccagc gcttgcccgc gcgggtcgcg gtgccgacca
     8941 cgcggcagac gaagtatgcg gcatcggaga ccagatagcg tccgggctca gccatgatac
     9001 ggatggtttc cggcacccgg gcaatcgctt cgttcaccac ttcgctgatt ttttcgattg
     9061 agggaatcgg cttcacatgg cgcaccggat agccgccgcc gatgttgaga agccgcggat
     9121 tcaagcccgc tacgcgcatt tgctggaaca ctttcagcgc gttctcgatg ccgacccgcc
     9181 agttctgcgg attgcggcac tgcgagccga catggaaagt gactcccgcg agatcggctt
     9241 tcagttcccc cgcggcgcgg atgatttcgc gtacttcggt cggatgcgcg ccgaacttgc
     9301 cggaaagcgg ccaatcgctg ccgatattgg gggtgtcgat gcgcaaatac agtttcgcat
     9361 cctgcttcac gctcacgatc ttgcgtagtt cttccacgct gtcaatcaca taccattcca
     9421 cgccctcggc caccgcatac tcgatgtact gcctcgactt catcgggttg ctgtaataga
     9481 ttcccccggc cgccacgccc aggccgagca gaatg
//
LOCUS       DATIOW010000225         5959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1214522, whole genome shotgun sequence.
ACCESSION   DATIOW010000225 DATIOW010000000
VERSION     DATIOW010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5959
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214522"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..398)
                     /locus_tag="VLL03_07445"
     CDS             complement(69..398)
                     /locus_tag="VLL03_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSS47230.1"
                     /translation="MIGRLNVPKLQASECSKARVSGLFFAVMLRDSKSWRFELSIVSL
                     RVLAQFFDINGRCEKCAATRLVHETKPLAELAEKEVDKHDVLLLMKVVTGAVHVVLSG
                     ALLMLQE"
     gene            complement(395..1960)
                     /gene="guaA"
                     /locus_tag="VLL03_07450"
     CDS             complement(395..1960)
                     /gene="guaA"
                     /locus_tag="VLL03_07450"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HSS47231.1"
                     /translation="MQHQKILILDFGSQYTQLIARRVREAHVYCELHPYDVGEKFIRE
                     FNPCGIILSGGPASVVGSGTPRAPQIVFELGVPVLGICYGMQTMAAQLGGAVENGLVR
                     EFGYAKVRAQGHSALLRGIQDQTNAEGHGLLEVWMSHGDRVNTLPTGFKVIAGNEAAP
                     IAAMADDSRRFYGLQFHPEVTHTPQGKRIIERFIHDICGAGQDWNMPDYVEEAVAKIR
                     SQVGKEEVILGLSGGVDSAVAATLLHRAIGKQLTCVFVDNGLLRLNEAEQVMNTFSRS
                     LGVKVIHVDASGKFLKKLSGVTDPEHKRKIIGKEFIGVFQREAKKLPRVKWLAQGTIY
                     PDVIESAGAKTKKAQSIKSHHNVGGLPETLHLKLLEPLRELFKDEVRELGLALGLPRE
                     MVYRHPFPGPGLGVRILGEVKKEYADLLRHADAIFIDELRASGWYEKTAQAFAVFLPM
                     KSVGVMGDGRTYEYTVALRAVQTQDFMTARWAELPHDLLSKIANRIINEVRGINRVVY
                     DISSKPPATIEWE"
     gene            complement(2040..3500)
                     /gene="guaB"
                     /locus_tag="VLL03_07455"
     CDS             complement(2040..3500)
                     /gene="guaB"
                     /locus_tag="VLL03_07455"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HSS47232.1"
                     /translation="MRVAKKALTFDDVLLVPAHSTILPRDVDLTTQLTRTIFLNIPLL
                     SAAMDTVTEARLAIAVAQEGGVGVVHKNMPPQAQAVEVAKVKRFESGVVKDPITISPE
                     MKVGEVMKLTQQHKISGLPVVDGSKVVGIVTNRDLRFETNLEQSVKSIMTPRERLITV
                     KEGASREEALKLMHKHRLERVLVVNDRFELRGLITVKDILKSSEHPYACKDELGRLRV
                     GAAVGVGEDTEERVGCLIEAGADVVVVDTAHGYAQGVLDRVKWVKKNFSATQVIGGNI
                     ATAEAAKAMVDHGADAVKVGIGPGSICTTRIVAGVGVPQITAIQDVSEALKNAGIPVI
                     ADGGIRYSGDIAKAIAAGAHAVMLGGLFAGTEEAPGEIELFQGRSYKTYRGMGSLGAM
                     QQGAADRYFQDPEMNADKLVPEGVEGRVPYKGSVVAVMQQLTGGLRASMGYLGCRTVA
                     EMRTQAKFVEITSAGVRESHVHDVQITKEAPNYRIE"
     gene            3689..4183
                     /locus_tag="VLL03_07460"
     CDS             3689..4183
                     /locus_tag="VLL03_07460"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HSS47233.1"
                     /translation="MPRVPSLIVLLLLSAVASAAGVYKWVDSDGTVHYSDQPPPGAVK
                     EQNLDNKSGTPPASSGASGTENAAPKAAGPKTYIEQNAEFRKRQVEAEEKRAKEEKAL
                     AEAKERQQNCERARSSLESLQSGQRVTKYNEKGERAYLDDNERAQEIVSAQKAVDSWC
                     NPRK"
     gene            complement(4190..4489)
                     /locus_tag="VLL03_07465"
     CDS             complement(4190..4489)
                     /locus_tag="VLL03_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HSS47234.1"
                     /translation="MQSDEIMIRVVYAAPLNQTVVELRVAKGATAGQAITQSGILQKH
                     PEIDLAKNGIGIFGQHVDAGAPLRDGDRVEIYRPLIADPKEARRRRAQANKRVRK"
     gene            complement(4479..4913)
                     /locus_tag="VLL03_07470"
     CDS             complement(4479..4913)
                     /locus_tag="VLL03_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HSS47235.1"
                     /translation="MTRVERIVLVPYTAQQMFALVDGIENYPQFLPWCSGTSVTRRDD
                     KVTAATLHINYHRLKLIFATENTKEMPRLIEMKLVSGPFRHLEGGWRFIELGGKGCKI
                     EFQLQYEFFSKLVEKLLSGAFQHIADSFVDGFIKRAEKIYAK"
     gene            4966..5415
                     /gene="smpB"
                     /locus_tag="VLL03_07475"
     CDS             4966..5415
                     /gene="smpB"
                     /locus_tag="VLL03_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HSS47236.1"
                     /translation="MSVIQNKKAFHDYFVEERYETGMVLAGWEVKAIRAGRCQIKEAY
                     VVVRKGEIFIIGCHISPLATASTHITPDPTRTRKLLLHTDEIGKLIGKVERAGYTLVP
                     LDIHYTRGKIKLEIGLAKGKKQHDKRETEKQREWQREKQRLLRAKRG"
BASE COUNT         1337 a         1785 c         1558 g         1279 t
ORIGIN      
        1 aagccgtaag ccgcagcctg ctatctcaaa attcgtcctt ttcctatgtc cgccgctgtc
       61 acgcgtccct attcttgaag catcaacaga gcgccactca acacgacatg gactgcgcct
      121 gttaccacct tcatcaacag gagcacatca tgtttgtcta cttccttctc agccagttca
      181 gccagtggct tcgtttcgtg gacaagccgc gttgcggcgc atttctcgca gcgtccgttg
      241 atatcgaaga actgggcaag aacgcgcaac gatacgatcg ataactcgaa tctccacgat
      301 ttgctgtcgc gaagcatcac ggcgaaaaaa agcccgctga cgcgggcttt tgaacattct
      361 gatgcttgta gtttcggtac gttcagacgt ccgatcactc ccactcaatc gtcgcaggcg
      421 gcttgctgga aatgtcatac accacgcggt tgatgccgcg cacttcgttg atgatgcgat
      481 tggcaatttt cgagagcagg tcgtgcggca gttccgccca gcgcgcggtc ataaaatcct
      541 gggtctgcac ggcgcgtaaa gccacggtgt attcataggt gcgcccgtct cccattactc
      601 ccaccgattt catcggcagg aacaccgcga acgcctgcgc ggttttttca taccagcctg
      661 aagcgcgcag ttcgtcaatg aaaatagcat cggcatggcg cagcaaatcg gcatattctt
      721 ttttcacttc gccgagaatg cgcaccccca gtcccggacc agggaaagga tggcggtaca
      781 ccatctcgcg cggcaatccc aatgccagac ccagctcgcg cacttcgtcc ttaaacaatt
      841 cgcgcaatgg ctcgagcaat ttcaaatgca gagtttcagg caaacctccg acgttgtgat
      901 gcgatttgat ggactgagct ttcttggtct tggcgccagc ggattcaatc acgtcggggt
      961 aaatcgtgcc ctgcgccagc cacttcaccc gcggcagttt cttcgcttcg cgctggaaca
     1021 ctccgataaa ttccttgccg atgattttgc gtttatgctc gggatcggtc acgccggaaa
     1081 gttttttcag gaatttcccg gacgcgtcca cgtgaataac cttgaccccg agactgcggg
     1141 aaaaagtatt catcacctgc tcggcctcat tcagccgcag caacccgttg tccacaaaca
     1201 cgcaggtcaa ttgcttgccg atggcgcgat gcaaaagcgt cgcggcgacc gccgaatcca
     1261 cgccgccaga tagacctaga attacttctt ccttgcccac ctgcgagcgg attttggcga
     1321 ccgcctcttc cacgtaatcc ggcatgttcc agtcctgccc tgcgccacaa atgtcgtgga
     1381 taaaccgctc gataatccgc ttgccctgcg gcgtgtgggt tacttcggga tggaattgca
     1441 ggccataaaa cctgcggcta tcatccgcca ttgcggcaat cggcgcagct tcgttgccgg
     1501 caatcacctt gaaaccggtt ggcagcgtgt tgacccggtc gccatggctc atccacacct
     1561 cgagcaagcc gtggccttcg gcattggtct ggtcctgaat gccgcgcaga agcgcggagt
     1621 gtccctgggc gcgcactttg gcatagccga attcgcgcac cagaccgttt tccaccgcgc
     1681 cgcccaactg cgccgccatg gtttgcatgc cgtaacaaat gcccagcacc ggcacgccga
     1741 gttcgaacac gatttgcggg gcgcgtggcg tgccgcttcc caccaccgat gccggcccgc
     1801 ctgaaagaat aatgccgcat gggttgaatt cgcgaatgaa tttttcaccc acgtcatagg
     1861 gatgcagctc gcaatacacg tgagcttcgc gcacgcggcg cgcaatcaac tgcgtgtact
     1921 gggaaccgaa gtcgaggatc aggatttttt ggtgctgcat ggctaagtga ggcgtaaggc
     1981 gcgaggcatg agaaacaatt ccatggggaa atatccgttt actcctcacc cctcactctt
     2041 cactcgattc tataattcgg cgcttccttg gtgatctgca catcgtgaac atgcgactca
     2101 cgtacacccg ccgaggtaat ttcaacaaac ttggcttggg tgcgcatttc ggcgacggta
     2161 cggcaaccaa gatagcccat gctggcgcgc aggcccccgg tcagctgctg cattaccgcc
     2221 actacgctgc ctttatacgg cacgcgccct tccacgcctt cgggcaccag cttgtcggca
     2281 ttcatctcag ggtcctggaa atagcggtcg gccgcgccct gctgcatggc acccagcgaa
     2341 cccatgccgc ggtaagtttt gtaagagcgg ccctggaaca actcgatttc acccggcgct
     2401 tcctcggtgc cggcaaacaa gccgcccagc atcactgcat gcgcgccggc ggcaatcgcc
     2461 ttggcgatgt cgccagaata gcgaatgccg ccgtcggcaa tcacaggtat acccgcgttc
     2521 ttcagcgctt cggacacatc ctgaatcgcg gtgatttgcg gcacacccac tccggcgacg
     2581 atgcgggtag tgcagatcga gcccgggccg atgcctactt tgaccgcatc cgcgccatgg
     2641 tccaccatcg ctttcgccgc ttccgcggtg gcaatgttgc cgccaataac ctgagttgcg
     2701 gaaaaatttt tcttcaccca cttcacgcga tccagcacgc cctgtgcata gccgtgggca
     2761 gtgtccacca caaccacgtc ggcgcccgcc tcaatcaggc accccacccg ttcttcggtg
     2821 tcttcgccga cacctaccgc cgcgccgaca cgcaagcggc ccagttcatc cttgcaggca
     2881 taagggtgtt cgcttgattt gagaatgtct ttcacggtaa tcagcccgcg caattcgaaa
     2941 cggtcgttca ccaccaacac ccgctccagg cggtgcttgt gcatgagctt cagcgcctct
     3001 tcgcggcttg cgccttcctt caccgtgatc aggcgctcgc gcggggtcat gatgctcttg
     3061 accgactgct cgaggttcgt ttcaaagcgc agatcgcggt tggtcacgat acccactact
     3121 ttggagccgt ccacgaccgg caatccggaa atcttgtgct gctgggtgag cttcatcact
     3181 tcgcccactt tcatttccgg ggagatggtg atgggatcct tcaccacgcc gctctcgaaa
     3241 cgcttgacct tcgccacttc caccgcctgg gcctgaggcg gcatgttttt gtgcacgacg
     3301 ccgacgccgc cttcctgcgc caccgcgatg gccaggcgcg cttcggtgac agtatccatc
     3361 gccgccgaaa gcagtgggat gttgaggaag atggtgcggg tcagttgcgt ggtgaggtcg
     3421 acgtcgcgcg gcaggatggt cgagtgggcg ggaacgagaa gaacgtcgtc aaaggttaat
     3481 gcttttttgg cgacccgcat gttttttcct ttgcaaagcg ttattatacg cacccgcagg
     3541 gaggcggtaa atatggcctg ccgcggacct ccagaaaaga tggggcgcac gggtcaaccc
     3601 gccgcaggag tcggacgttg aaatgactgt tcattgccaa ccaaatgaca tacactaaat
     3661 gccccatgtt tgccaccgga gctcaatcgt gccacgcgta ccaagcttga ttgtcctgtt
     3721 gcttttgtct gcggtcgcgt ccgccgccgg cgtctataaa tgggtcgatt ccgacggcac
     3781 cgtccactat tccgatcagc cgcctccagg cgccgtcaaa gaacaaaacc tggacaacaa
     3841 atccggaaca ccgccggcca gttccggggc ttctggcacg gagaatgccg cacccaaggc
     3901 agccggtccc aaaacctaca tcgaacaaaa cgcggaattc cgcaagcgcc aggtggaggc
     3961 cgaggaaaaa cgcgcaaaag aagaaaaagc gctggccgaa gccaaagagc gccagcaaaa
     4021 ctgcgaacgc gcgcgcagca gtcttgagtc cctgcagtcg gggcagcgcg tcaccaaata
     4081 caatgaaaaa ggcgagcgcg cctacctcga tgacaatgaa cgcgcacagg aaatcgtgag
     4141 tgcgcaaaaa gccgtggatt cctggtgcaa tccgagaaaa tgacccggat tatttcctga
     4201 ctcttttgtt cgcctgcgcc cgccgcctgc gggcctcttt cggatccgca atcaagggac
     4261 ggtaaatttc cacgcgatcg ccgtcacgca atggtgcgcc cgcatcgacg tgctggccga
     4321 aaatcccgat accattcttg gcgagatcaa tttctgggtg tttttgcaga attccggatt
     4381 gcgttatggc ctggccggcg gtcgcacctt tcgccacccg caactctaca accgtctggt
     4441 tgaggggcgc ggcgtaaacc acccggatca tgatctcatc actttgcata tatcttttcc
     4501 gcacgcttga tgaagccgtc cacgaaacta tcggcgatgt gctgaaacgc gccgcttagc
     4561 agcttttcca caagtttgct gaaaaattca tattgcagct gaaattcgat tttgcagcct
     4621 ttccctccga gctcaataaa acgccagccg ccctcgagat ggcgaaaagg cccgctgact
     4681 agtttcattt caatcaaacg cggcatctcc ttggtatttt cggtcgcaaa gatcagcttc
     4741 aagcgatgat agttaatatg cagggttgcg gcagtgactt tatcatcacg gcgcgtgacc
     4801 gatgttcctg aacaccaggg caggaactgc gggtagttct caatcccgtc caccagcgca
     4861 aacatctgtt gcgcagtgta cggaaccagc acgattcgct caacgcgggt catcgaattc
     4921 caaaagctga taaaattcac agaattttag caaaccgccc tcttcatgag cgtcatccag
     4981 aacaaaaaag ccttccacga ctatttcgtc gaagaaagat acgagacggg catggtcctt
     5041 gcggggtggg aagtcaaggc gattcgcgcc ggccgctgcc agatcaagga agcctatgtc
     5101 gtggtgcgca agggcgagat tttcatcatc ggctgccata tcagtccgtt ggcgaccgca
     5161 tcaacccata tcacgcccga ccccacgcgc acccgcaaac tactgctgca cacggatgaa
     5221 atcggcaagc tgatcggcaa agtggagcgc gccggctaca cactggtgcc gctcgacatt
     5281 cattacacac gcggcaaaat caagctggaa atcgggctcg ccaagggcaa aaaacagcac
     5341 gacaaacgcg aaacggaaaa acagcgcgaa tggcagcgcg agaaacaacg tctgttgcgc
     5401 gccaagagag gatgagcaat taaatttatg gggtggacga tgggattcga acccacaaca
     5461 actggaatca caaaaattta atatagagtt tataacatcg cagctatttt ataaatcaat
     5521 accttgcaat gcccgccgct ttcatattta gtgtcgcgat gtctcgaaaa gcttcacaaa
     5581 aggggaaaag agttacgtaa tggttacgcg gtgctagcgc atgagtggct ccccgaatag
     5641 ggctcgaacc tacgacctgc agattaacag gcattaaaag tagctatttt taaactaagt
     5701 taggcccgcc ctcgctcgcc aacgatcgcc ggcactcgct gagcgtgcta cgtttttact
     5761 acacttcttt ggctccccaa gccgggcaaa tctgagaact cttgggtgtt aagggtgcca
     5821 ctcagctgat cgtgcgccgg acaggatcaa ctggacactg gcttgagttc attgtagtgg
     5881 cctgttagcc tctcgaacgg caggatacgg ccaaaagcgg tcattcgcgg gtaaccgaag
     5941 ttaatgccta aaatgattt
//
LOCUS       DATIOW010000226        32388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_198759, whole genome shotgun sequence.
ACCESSION   DATIOW010000226 DATIOW010000000
VERSION     DATIOW010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 32388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32388
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198759"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..653)
                     /locus_tag="VLL03_07480"
     CDS             complement(63..653)
                     /locus_tag="VLL03_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HSS47237.1"
                     /translation="MDLIARINGHFSDSAHLKLQAMDALAAPIASAAERMAQCLMHDG
                     KILSCGNGGSAADAQHFAAEMLNRFEMERPGLAAMALTTDTSTLTSIANDYSYDQVFS
                     KQVLALGQANDILLAISTSGNSRNVIEAVRAAHERQMHVVALTGKSGGQLAEILRSDD
                     IHVCVPANNTARIQEVHLLTLHCLCDAIDCLLMGVE"
     gene            complement(716..1066)
                     /locus_tag="VLL03_07485"
     CDS             complement(716..1066)
                     /locus_tag="VLL03_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HSS47238.1"
                     /translation="MNRSADGVAAEKHAATFLQKQGLKLLETNYRCRFGEIDLIMRDG
                     DTLVFAEVRLRKNQNFGGAAASITAQKQARLVKTARHYLSSLKTPPACRFDAVLLSET
                     GGREIEWIKNAFEE"
     gene            complement(1050..2201)
                     /locus_tag="VLL03_07490"
     CDS             complement(1050..2201)
                     /locus_tag="VLL03_07490"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HSS47239.1"
                     /translation="MQRFPAFLLTLLALLYGATVAFATRAQSIAPPAAAKPLPAQAVT
                     ATKPEANLQTNPPKPHIALILPLKSEGFGNAAELTRQGFLAAASHQGNTSLPIKTYAT
                     TEQIDEILSAYQLALAAEAQVVVGPLTRDGVSALARSGMVRVPTLALNLPDDFTALPA
                     NLFTLSLHVENEAQQAASLAAQEGRRKALIITTDTPLSQRMQQAFADAWQKLGGVILQ
                     QLVYISDPGGLQTLRQAVTISGADTIFLALDARRARLVRPYLDANANIYATSQIFSGN
                     TQGPLNFDLNGIRFVDMPWVLQSDHPAVMIYPRPNRPVIVDLERLYALGIDAYRIAQD
                     LLNARPGLGYTLDGVTGQITLNAENQFVRELLPAMFYQGQAVVLYEPKR"
     gene            2196..3071
                     /gene="rsmI"
                     /locus_tag="VLL03_07495"
     CDS             2196..3071
                     /gene="rsmI"
                     /locus_tag="VLL03_07495"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HSS47240.1"
                     /translation="MHRGQNDKGEPLLYVVATPIGNLQDISLRALDVLKRVDVVAAED
                     TRNTSRLLKRFAIAVKLIALHEHNEKSAAARIVALLRSGKFVAVVSDAGTPAISDPGA
                     LVVERVRVAGFRVVPVPGANAAVTALSAAGLESSHFLFYGFLPSKPSARLQVLQNIKT
                     LPYTLVFYEAPHRIIECVSDMQQALGGERRVVIARELTKLFETIHSCTLGEAAEWLNA
                     DPNRLKGEFVLLVSGVELVPASTNITDAERILKLLLAELPLKRAVKLTAGISGANKKT
                     LYALALRIKGTAKQV"
     gene            3082..4119
                     /gene="pyrC"
                     /locus_tag="VLL03_07500"
     CDS             3082..4119
                     /gene="pyrC"
                     /locus_tag="VLL03_07500"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HSS47241.1"
                     /translation="MALQKITITRPDDWHVHLRDGAPMRAVLADTARRFGRAIVMPNL
                     KPPVTTTEAALAYRKRILAALPKSVKFVPLMTLYLTDKTSPQQIIKARHSSIVHAAKY
                     YPAGATTHSGAGVTDIKKCSAALEEMQKQDLPLLVHGEVVDSEVDVFDRERVFIERVL
                     TPLLLRFPKLRIVFEHITTREAVEFVRSTPANIGATITAHHLLLNRGGIFSGGIRPHH
                     YCLPVLKRENHRLALVEAAIGGSPKFFLGTDSAPHAQSAKESACGCAGIYTAHAAIEL
                     YAEVFERANALDKLEGFASFHGADFYRLPRNTDTITLVKQSKRVPKSIKFGKDSLVPL
                     RAGEEVSWRML"
     gene            complement(4239..4751)
                     /locus_tag="VLL03_07505"
     CDS             complement(4239..4751)
                     /locus_tag="VLL03_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013951778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HSS47242.1"
                     /translation="MRRRLYFVLPDLASAKQTMNDLLLARIEERHIHCLARRGTPLDD
                     LHEANILQKTDIVHGAELGMTLGGVGGAALGIIMVFSPPEGVTLQLVTVLIASLIGAL
                     LGAWVSSLVASAVPNSKLLLFAKDIEEGKILIMVDVPARKVEQISQLVERCHPEAAPG
                     GLEPTIPAFP"
     gene            4890..5219
                     /gene="rnpB"
                     /locus_tag="VLL03_07510"
     ncRNA           4890..5219
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VLL03_07510"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            5492..5926
                     /gene="mraZ"
                     /locus_tag="VLL03_07515"
     CDS             5492..5926
                     /gene="mraZ"
                     /locus_tag="VLL03_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HSS47243.1"
                     /translation="MAQLSLDNKGRLAIPTKYREGLATRGNGRLVITADPDACLLLYA
                     QPDWEPIQQKLNSLSSFNPKLRNLQRVIVGYAEDIEMDGTGRILISPSLREFAAFDKR
                     VVLVGQGMKFELWDAEKWNEQRNLAQSFRNQPLPPELEGFSL"
     gene            5929..6858
                     /gene="rsmH"
                     /locus_tag="VLL03_07520"
     CDS             5929..6858
                     /gene="rsmH"
                     /locus_tag="VLL03_07520"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HSS47244.1"
                     /translation="MSSQHATVLLNEAVEALKVRPNGVYVDCTFGRGGHSRLLLEMLG
                     KNGRLVALDKDPAAVKAARTLKDARFCIVHRRFAALREVLYELGIKHVDGVLMDLGLS
                     SPQLEDAARGFSFRRDGPLDMRMDISRGETAAEWLERANEEEIGEVIKKYGEERFAKQ
                     IARAIVAARKAGPVDTTRQLAQIVAAAVRTREPNQDPATRTFQALRIYLNQELEELSL
                     VLPQTVDALKPGGRLVVISFHSLEDRMVKRFMRDEARADDLPARLPLRTNEMRSPKLH
                     VIGKTVRPGVREIAANPRARSAVMRVAERTEEV"
     gene            6911..7192
                     /gene="ftsL"
                     /locus_tag="VLL03_07525"
     CDS             6911..7192
                     /gene="ftsL"
                     /locus_tag="VLL03_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HSS47245.1"
                     /translation="MTRLNLILVLILIGCALGVVTSQHKARKLFVELQKEQEWAQQLE
                     VEWGQLQLEQSTWATHARIERIAGKSLQMHVPDAARTQLVTPPEEKAHE"
     gene            7185..8915
                     /locus_tag="VLL03_07530"
     CDS             7185..8915
                     /locus_tag="VLL03_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HSS47246.1"
                     /translation="MNSLANPVLTLRFPVWRSRVVLLALLLWFIGLGARAVYLQGMHN
                     DFLQQKGDARYSRVIEISAHRGMITDRNGEPFAISTPVESVWASPADVEITPEKLKQL
                     AGLLESDKTEVGKRLKESNKEFVYLKRQISPELAARVVQLGIPGVFLQREYHRYYPAG
                     EVMAHLLGFTGMDDNGQEGLELAYQDWLAGKPGSRRVIKDRLGRIVEDVESISAPQEG
                     RTLTLSIDSKIQYLAYRELKQAVEANHAKAGGIVVLDAKSGEVLALANVPSYNPNNRI
                     RLNLQRTRNRAITDLFEPGSTLKPFTIAAALESGQYTPESMIEIAPGQFSVGNATIHD
                     THREAVLTVAQIIQKSSNIGAAKIALSLSSHTLWNLFSRVGFGVVPHSGFPGEVSGKL
                     RPYKSWRPIEQATMSYGHGISVSLLQLARAYTVFAADGELKSLSLAKLDAPQSGGQVI
                     SPATARAVRDMLEMVVEPGGTAPRAQIVGYRVAGKTGTAHKLENGSYTTDRYVSSFVG
                     MAPASDPRLIVAVMLDEPSAGQYYGGRVAAPVFSQVMAGSLRMLGVAPDAPTNNVVLP
                     DDFPEIKEEV"
     gene            8957..10423
                     /locus_tag="VLL03_07535"
     CDS             8957..10423
                     /locus_tag="VLL03_07535"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HSS47247.1"
                     /translation="MPRFDARVLNRLGVKTKRLATDSRQIKPGDTFIAYPGEKLDGRN
                     YIPRAIAAGAGAVLWEKRDFKWNPQWKAANLGVNGLREKASVIANRIYGRPSHKLWVI
                     GVTGTNGKTSCSHWIAQCLTRLGKKTAVVGTLGNGFPGRLEPTVNTTPDAVLLQELLA
                     GFLQRGAQCVVIEVSSHSLTQGRVNGVAFDVAVFTNLSRDHLDYHGSMQAYAAAKAKL
                     FHWPGLRCAIVNLDDRFGRELARQLEKRRVKVLGYGFGKGDIAGHRLDLARRGLSLEI
                     RTRWGGARLQSRMLGKFNAANLLAVLGVLLVSGVKLEPAVRALQEIKPPSGRLQIVKD
                     QPLVVVDYAHTPDALKKVLQSLRSIAQESSGKLVCVFGCGGDRDRGKRPQMGRVAVSL
                     ADQVVVTSDNPRSEDPLAIIADIARGWLPGFKEPERAPVVEPDRAKAIRLAISGAAEN
                     DVVLIAGKGHEDYQEIGGEKMPFSDVRTAQTVLQESAG"
     gene            10462..11781
                     /gene="murF"
                     /locus_tag="VLL03_07540"
     CDS             10462..11781
                     /gene="murF"
                     /locus_tag="VLL03_07540"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HSS47248.1"
                     /translation="MRGNDVRFTAVSTDSRKAATGDLFVALRGEHYDGHDYINAARGQ
                     GAVAVMVDKAERAENCPALVVEDTRQGLVGLACYWRSKFSLRLAAITGSNGKTTVKEM
                     LAAILRKHAGADAVLATQGNLNNDIGVPLTLLGLRPPHRYAVVEMGMNHPGEIANLAR
                     IAKPDVALVNNAGPAHLLGLGSVEAVARAKGEIFEGLPDNGVAVINADDAHANLWRQL
                     CQGRRVLDFGFEHPAAIKGRCELKNDSSEIALQTDAGGIYLKLQVPGLHNARNALAAA
                     ACARALGVQREAIAAGLAEFAGVKDRQQRKAGKKGAIVIDDSYNANPDSVKAAIDMLA
                     GLSGMRVLVLGDMGELGEQAPQFHARIGAQAKAAGIDRLYALGALSAEAARQFGEGAG
                     HFTQINDLISEVEKWMAPNVTVLVKGSRFMQMERVVERIVAGQEAGH"
     gene            11781..12866
                     /gene="mraY"
                     /locus_tag="VLL03_07545"
     CDS             11781..12866
                     /gene="mraY"
                     /locus_tag="VLL03_07545"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HSS47249.1"
                     /translation="MLLALAQWLAQDIRTFNVFNYITLRAVLATLTALLISFMVGPAM
                     IRKLAVYKIGQAVRDDGPKSHFAKAGTPTMGGALILVSIGFTTLLWADLRNRFVWVVL
                     VVTMGFGLIGWVDDYRKVVRRNPKGLSPRTKFFWQSVIGFAAALFLAFSTTLPAQTTF
                     IVPFFKHVVYPLGIFGFVGMTYFVIVGSSNAVNLTDGLDGLAIMPTVMVGSALGVFAY
                     VAGHAVFSKYLGFPYIPGAGELTVFCAALAGAGLAFLWFNAYPAEVFMGDVGALALGA
                     ALGTIAVIVRQEIVLFIMGGVFVVETLSVMLQVASFRLTGKRIFRMAPLHHHYELKGW
                     KETQVVVRFWIITMMLVLVGLSTLKLR"
     gene            12867..14270
                     /gene="murD"
                     /locus_tag="VLL03_07550"
     CDS             12867..14270
                     /gene="murD"
                     /locus_tag="VLL03_07550"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HSS47250.1"
                     /translation="MEIELKGKKALVVGLGDTGLSLARWLTRKGATVRVVDSREAPPR
                     AAQLKVELPQLALDTGPFHEESFRDIDMIALSPGVALREPRISEALNAGVFVCGDVEL
                     FAQALKKMPLTRVLAITGSNGKSTVTSMTGEMCRAAGLNTVVAGNIGLPVLDALTQAE
                     NQVQRGKRPDVFVLELSSFQLETTYTLEPGAAAVLNLSEDQLDRYPSMAEYAAAKARI
                     FAGSGVQVLNRDDRQSFTMAGKERRVFSFGLGRPLREMEWGLLEVAGEKWLAQGEMKL
                     MPVKELPLTGLHNAANALAALALCRAIGIEYAPLLHALKKFRGLPHRVQKIADIGGVG
                     FYDDSKGTNVGATVAALNGMQQKVVLIAGGDGKGQDFSPLKTAITGHARALVLIGRDG
                     EKIAQAVAGGNVPMRRATSMREAVALSFSLAQRGDAVLLSPACASFDMFRNYEHRAEA
                     FAQAVRELQKQPRVGAN"
     gene            14271..15425
                     /gene="ftsW"
                     /locus_tag="VLL03_07555"
     CDS             14271..15425
                     /gene="ftsW"
                     /locus_tag="VLL03_07555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HSS47251.1"
                     /translation="MFYAAPAGRAPLPAYDQSLLGATLALLGLGLVMVYSASIAIAEA
                     GRWHQPAYFLIRHTVFLGISVVLGAIAFQFPMYLWQRVSPWLFVLGAGLLVLVLIPGV
                     GREVNGSQRWLPLGLVHLQPSEFMKLIVVIYAADYTVRKAAYMHSFRKGFLPMLMVML
                     LIGGLLLREPDFGAFAVITTIAMAILFLGGMNWRLFALLIVLLMVGFLILIWTSPYRM
                     QRIIGFMDPWSDPFGKGYQLSHALIAFGRGEWLGVGLGGSVEKLFYLPEAHTDFLLAV
                     VAEELGFAGVAAVIALFTWLVARAFAIGRQAAYLERYFSALVAQGVGVWLGVQAVINI
                     GVNMGVLPTKGLTLPLMSFGGSGIAANCVAIALLLRVDWENRQLKKGKPL"
     gene            15426..16502
                     /gene="murG"
                     /locus_tag="VLL03_07560"
     CDS             15426..16502
                     /gene="murG"
                     /locus_tag="VLL03_07560"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HSS47252.1"
                     /translation="MGRTILIMAGGTGGHVFPGLAVAEYFKKQGWRVVWLGTRDGMEA
                     KLVPQQGYDMAWVNFSGVRGKDVLRLVLLPLQLIIAFWQSAQAIFRHRPEVVLGLGGY
                     PAFPGGMMAVLLNKPLAIHEQNSIAGLTNKVLAKLADKVLVAFPEAFGAAEPRNQIWT
                     GNPVREAIAQLAPPQMRYAGRTGPLRLLVVGGSLGAQALNENVPQALALLPKDERPLV
                     THQSGAQHVDALKQAYRRAALEVRIEAFIDNMAQCYAESDLVICRAGALTIAEIAAAG
                     VASILVPYPHAVDDHQTHNARFLADQGAAMLLPQTELNPNSLAGLIKSLSRGKLLAMA
                     SRARELGKPDATRKVAEICMELAR"
     gene            16666..18066
                     /gene="murC"
                     /locus_tag="VLL03_07565"
     CDS             16666..18066
                     /gene="murC"
                     /locus_tag="VLL03_07565"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HSS47253.1"
                     /translation="MRHKVKRVHFVGIGGSGMSGIAEVLLNLGFVVSGSDLSENAVTQ
                     RLKSLGATVYKGHDAAHIERADAVVFSTAVKADNPEVTAARNHKIPVVPRAMMLAELM
                     RLRQGIAVAGTHGKTTTTSLIASVLAEAGMDPTFVIGGRLEAAGSHAKLGGGEFIVVE
                     ADESDASFLYLQPVLAVVTNIDADHMETYAHDFRKLTQAFVDFLQHLPFYGTAVLCSD
                     DARVREIIPHLTKPVTTYGLSQSAQIRAVDVKHHGGQMQFRVVRTSGKDLQITLNMPG
                     VHNVQNALAAIAVGIELGVADSAMAKALAEFKGVGRRFQRYGEVPLPRGGNFTLIDDY
                     GHHPAEMAATLAAVRGAFPGKRVVLAFQPHRYTRTRDLFEDFVKVLSAVNVLLLCEVY
                     PAGEAAIPAADGRTLVRAIRVHGKVEPIFVENIAELPQVIFNAAQAGDVVVTMGAGSI
                     GGVPGVLARPTVSSSA"
     gene            18063..18983
                     /gene="murB"
                     /locus_tag="VLL03_07570"
     CDS             18063..18983
                     /gene="murB"
                     /locus_tag="VLL03_07570"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HSS47254.1"
                     /translation="MNMAETNHLAGLRGELRFNEPMAEHVSWRAGGRAARAYIPADLA
                     DLVAFFETCGKNEPVYIVGLGSNLLVRDGGVRGTVVLLHSGLKQLRLESREAGCAMIY
                     AEAGMACPKVARFAATHDLHGAEFLAGIPGTVGGALAMNAGCYGSETWEIVAQVLTLR
                     RDGTLSMRTPADYRIGYRSVAPHSKLEEWFAAAWFRLPQGDSKAAKEKIRDLLKRRIA
                     SQPLELPNAGSVFRNPQGVYAARLIEQCGLKGLRFGGAEVSAKHANFIVNTGGATAAD
                     IEALIQKIQLTVAQECGIKLETEVRIIGEA"
     gene            18980..19897
                     /locus_tag="VLL03_07575"
     CDS             18980..19897
                     /locus_tag="VLL03_07575"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HSS47255.1"
                     /translation="MKGKKLGKVAVLMGGRSAEREISFKSGTAVLNALHKRRVNAHAF
                     DPSARRLEDLRSGGFERVFIALHGRQGEDGTVQGALQLMGIPYTGSGVLGSALAMDKW
                     RTKLVWRAAGVGTPRYELLDEKSDFRKIAKKLGLPIMVKPVCEGSSIGMSKVMKAEKL
                     RAAWRLAAKYDPVVIAEQFIKGVELTVSILDGEALPLIRLETPRTFYDYQAKYFSRTT
                     RYILPCGLSAPQEKKIQQKALHAFRILGCSGWGRVDLMLDKRGRPYFLEANTVPGMTD
                     HSLVPMAARAAGLSFEDLVLRILEQAHVE"
     gene            19887..20630
                     /locus_tag="VLL03_07580"
     CDS             19887..20630
                     /locus_tag="VLL03_07580"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HSS47256.1"
                     /translation="MWNKSYAMLWLANLLYTLAGLLALYCALYFAIRLPWFELKTVQV
                     RGELTHASQEQLLAVVRGLKGNFFTVDLDEARSRFEKLPWVRGVGLRRQWPDRLEVAL
                     EEQVPVARWSNGGLVNTYGEVYAAEADADLPLFSGPAGTSQDIAKAYAAFKQMLEPIQ
                     RVPVQVRMTTRRAWQIQLDDGSTLALGRERMDARLKRFVAAYDRTLGGLQWQSDYVDL
                     RYDNGFALRVPEAVREQSEPAKREKHKAA"
     gene            20652..21890
                     /gene="ftsA"
                     /locus_tag="VLL03_07585"
     CDS             20652..21890
                     /gene="ftsA"
                     /locus_tag="VLL03_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HSS47257.1"
                     /translation="MGRSKENKDLIVGLDIGTSKIAAIVAEMRPEGGLEIIGMGSHLS
                     RGLKKGVVVNIESTVNAIQRALEEAELMADCKIREVYTGIAGSHIKSFNSHGMVGIKD
                     KEVSQMDVDRVIETAKAVNIPTDQQILHILNQEFIIDGQEDVREPLGMSGVRLEVKVH
                     IVTGAVSAAQNILKCVRRCGLSVRDLILQPLASAIGVLSEDEKDLGVCLADIGGGTAD
                     IAVFHHGAIRHTAVIPIAGDQITNDIAVALRTPTKDAEDIKRRYGCALRQLADPQQMV
                     EVPGVGERGPRQLSRQTLAEVIEPRVEELYSLVQTELRRSGFEELLSSGIVITGGSSA
                     MQGMVELGEEVFHMPVRLGLPHYLGGLVEVVRHPRFATGVGLLLLGMQQQQRQELAKL
                     QSGSLKQVFERMKRWFQGNF"
     gene            21999..23156
                     /gene="ftsZ"
                     /locus_tag="VLL03_07590"
     CDS             21999..23156
                     /gene="ftsZ"
                     /locus_tag="VLL03_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HSS47258.1"
                     /translation="MFEIVDSEAKEAQIKVVGVGGCGGNAVDHMIYRGVKGVEFISAN
                     TDVQALRRNQAKIQLQLGNSITKGLGAGANPDIGREAALEDRERITELIQGADMLFLT
                     AGMGGGTGTGAAPVMAEIARDLGILTVAVVTKPFAFEGKRMGIARQGIEALSQFVDSL
                     IVVPNDKLMQVLGNGVTLDAAFQAANDVLHGAVAGIAEIISCPGMINVDFADVRTVMS
                     EMGMAMMGSAKAVGADRAKLAAEQAVACPLLEDINILDARGVLVNISASRDSFQLQEM
                     YDVMDTIKTFAAESATVIVGAVYDEELKDSLRVTIVATGLNGPVNRMQEKPLSVVKTG
                     THDAPVEMVNYAELETPAVVRRRNRDATIEAMRQSGVEMLDIPAFLRKQAD"
     gene            23273..24184
                     /gene="lpxC"
                     /locus_tag="VLL03_07595"
     CDS             23273..24184
                     /gene="lpxC"
                     /locus_tag="VLL03_07595"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HSS47259.1"
                     /translation="MIKQRTLKNIIRATGVGLHTGEKVYLTLRPAAPNTGIVFRRIDL
                     KQPVEIKADPHSVGDTRLSSCLEKNGVRVATVEHLMSAFAGLGIDNAYVDLTAPEVPI
                     MDGSASPFVFLLQSAGIEEQRVPKEFIRIKKMVEVVDGDKWVRFEPYNGFRVNFSIDF
                     DHPVFEPSGQSVTVDFANTSYIKEVSRARTFGFMQEVETLRSMGLALGGSLDNAIVMD
                     EYRVLNSDGLRYEDEFVKHKVLDAIGDLYLLGHPLIGAFTGHKSGHTQNNALLRALLA
                     DAQAWEFVSFESSEEAPAFLRLQTQFA"
     gene            complement(24386..24880)
                     /locus_tag="VLL03_07600"
     CDS             complement(24386..24880)
                     /locus_tag="VLL03_07600"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HSS47260.1"
                     /translation="MPVRKLGFYFDTNPAMQSLVKEASRLIEMQKVFAEIAPSPLARS
                     GRVGRFAHGSLLLLADNGAVAAKLKQLVPSLLVKFQKRGYEVTAIRVTAQPPGRAAVP
                     HKNIRLSREASGYLRELAASLPITPLRTALEKMAARPSDDDDQPFQDKESQDQDGQNH
                     EKPQ"
     gene            24908..25828
                     /locus_tag="VLL03_07605"
     CDS             24908..25828
                     /locus_tag="VLL03_07605"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HSS47261.1"
                     /translation="MNIILVSGNLGKSRTTTLSHSQIVFTAVLLLIGIVTFAIALHYL
                     TLLRAAEIKSPYLQALLVSVQEQELRKQQAYLRENLNAMAVKLGQMQAQLLRLDALGD
                     RLAKQAGFKPQEFMFDQLPGRGGAVSGTPQRDLTLNEFNQQMERLSSELSDRTDKLGI
                     LETALIQDRLKQKLVPSVPPVDTGWFSSNFGWRIDPFTGSNAFHEGVDFMSEVGSTVV
                     AAAGGVVVHSSDNNSEYGSMIEIDHGNGLVTRYAHLSKRLVKVGDVVLRGGKIGEVGN
                     TGRATGPHLHFEVRFKGAPQNPVRFLHKPG"
     gene            25919..28708
                     /gene="secA"
                     /locus_tag="VLL03_07610"
     CDS             25919..28708
                     /gene="secA"
                     /locus_tag="VLL03_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HSS47262.1"
                     /translation="MLASLLKKIFGSRNERLLKQYTHTVRAINALEPAIEKLSDAELR
                     AKTDEFKGRIRERLATIPDHADGDAERAQAQEEERSRARKAILEELLPEAFAVVREAS
                     KRTLQMRHFDVQLIGGMVLHYGKIAEMRTGEGKTLVATLPAYLNALTGRGVQVVTVND
                     YLASRDAEWMGRIYNFLGLTVGVIVSQQEDAAKRAAYAADITYGTNNEFGFDYLRDNM
                     KYAVEERAQRGLHYAIVDEVDSILIDEARTPLIISGQADDTTDLYHRINDLVPKLTRQ
                     QQENGPGDYSVDEKTNQVLLTETGHERAEQLLSQAGLLPPGGSLYDSAHINLVHHLYA
                     ALKAHALFHRDQHYVVQNAEVVIVDEFTGRLMAGRRWSDGLHQAVEAKEGVKIQRENQ
                     TLASITFQNYFRMYKKLAGMTGTADTEAYEFQQIYGLETVVIPTHKPMIREDRQDQVY
                     RTTKEKYTAVINDIRDCCERGQPVLVGTTSIENSELLSGLLDREKLPHQVLNAKQHAR
                     EAEIVVQAGRPKWITIATNMAGRGTDIVLGGNPEPEINKIRSDANLDDASKAARIEEI
                     RAQWQKLHEQVVAAGGLHIIGTERHESRRVDNQLRGRSGRQGDPGSSRFYLSLEDPLL
                     RIFASDRVSAIMQRLNMPEGEAIEHPWVTRAIENAQRKVEARNFDIRKQLLEYDDVAN
                     DQRRVIYQQRSELLESTDISDTIRAMREDVVNDLISQHIAPESVEEQWDVSGLEKTIA
                     VELQLNLPLQLWLKQDPELHEETLRSRIIEAAHAQYQGKLEQVGTEVMHQYERAVMLQ
                     SLDSHWREHLAALDHLRQGIHLRGYAAKNPKQEYKREAFELFAGMLELIKAEVTKIIM
                     TVQIRSREEVEAVEPPPQPENVQYHHADYDEALAQAGNAEQKPFVRQGQKIGRNDPCP
                     CGSGRKYKHCHGKLS"
     gene            28757..29662
                     /locus_tag="VLL03_07615"
     CDS             28757..29662
                     /locus_tag="VLL03_07615"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HSS47263.1"
                     /translation="MQTWNLHLYQFMNDWRLFRRSYLLKFMLVAFLGTHVPLIVLVLY
                     IALSQSYSLMGALPIVIATLFATLIGAAITLLFLGSLLLPVRQTSESLKQYLAERNLP
                     SLPTIYGDEAGQLMADTQYVIMRLDNALRELENISLTDHLTGVYNRRAGEQRLNEEIA
                     RAERENKHFLLAFIDINSLKKINDQYGHDAGDACIIHVATLLAENIRRGDWCARWAGD
                     KFILAVYHPDISTELVFNRVLAKLQAKPCLVRHNVTLNLSVSIGVAVYQRGVAVETLV
                     QQADQAMYQAKLLGQDQSHVAFCGK"
     gene            29719..30948
                     /gene="argJ"
                     /locus_tag="VLL03_07620"
     CDS             29719..30948
                     /gene="argJ"
                     /locus_tag="VLL03_07620"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HSS47264.1"
                     /translation="MPVNLHSPQPEQVLPVKGVALGVAEAAIKKPGCKDLLVMVFEPG
                     AKVAGLFTQNRFCAAPVLLAKKHLSSGLPVEALVVNTGNANAGTGEDGIKRARRVCAE
                     LAKARDCETEQILPFSTGVIMEPLPVERIVAGLPQCLAGLRADNWLAAAQAIMTTDTV
                     PKARSKQISINGATVTITGIAKGAGMIRPNLATMLAFIASDARVSQAALQAVVSHAAA
                     HSFNCITVDGDTSTNDAFMLIASGQAPMPEIAAATSREFTAYRDAVTGVAQWLAQSIV
                     RDGEGATKFITVRVEQGKDAAECARVAYAIAHSPLVKTAFFAADPNLGRILAAIGYAG
                     IADLDVNKVSLYLDEVLVAENGGRAASYNEAAGRKVMSRPEIALRVRLGRGQADYTVW
                     TCDLSHDYVTINAEYRT"
     gene            31006..31860
                     /locus_tag="VLL03_07625"
     CDS             31006..31860
                     /locus_tag="VLL03_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSS47265.1"
                     /translation="MLLRIGDLLGRLELVLPAAPRPPDWQASIAFRWRQRYGRGFLQP
                     VPHPHRIRLSDLLGIEQQKQLIDQNTRQFIQGFPANNVLLTGARGTGKSSLVKAVLNK
                     YAGKGLRLIEVDKHDLIDLPDIVEPVCRRPERLILFCDDLSFEADEPGYKALKAALEG
                     SITAASENMLVYATSNRRHLMPEYMEENLATRHVGDEVHPAEAVEEKISFSERFGLWL
                     SFYPFSQDDYLGIVDHWLGFLGVRHADTDEVRQAAVQWALARGSRSGRAAWQFARDWA
                     GRHALKGK"
     gene            31857..>32388
                     /locus_tag="VLL03_07630"
     CDS             31857..>32388
                     /locus_tag="VLL03_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HSS47266.1"
                     /translation="MKSAVGSSNAPADPRQLVKVAAAVILRPDGQFLLAQRPAGKPYP
                     GYWEFPGGKMEAQESSKQALERELKEELGIHIEEIYPWITCIYHYTHASVELHFHRVV
                     RWTGQLHGRESQQLAWQSVKAVEVAPLLPANSAVLRALTLPEHYAISNVAELGREKFM
                     RRLERSLQGGLRLVQLR"
BASE COUNT         6773 a         9017 c        10276 g         6322 t
ORIGIN      
        1 cgcaacacag ccttgcaaag cgggaataat caaaaccaga gaaataaacg gtaaatagcg
       61 catcattcaa ctcccatgag taaacaatcg atcgcatcgc acagacaatg caaggttaaa
      121 aggtgaactt cctgaatgcg cgccgtattg ttggccggca cgcagacatg gatgtcgtca
      181 gatctaagta tttccgccag ctggcctccg gattttccgg tgagggcgac cacgtgcatc
      241 tgccgctcgt gcgcggcgcg taccgcttca atcacgttgc gcgaattgcc ggaggtggag
      301 atggcgagca ggatgtcgtt ggcctgcccc agcgccagta cctgcttgga gaacacctgg
      361 tcgtagctgt aatcgttggc aatcgaggta agcgtggagg tatcggtggt cagcgccatg
      421 gcggccagcc ccggtcgctc catttcaaag cggttcagca tttccgccgc gaagtgctgc
      481 gcgtccgccg ccgagccgcc gttgccgcaa ctcaggattt tcccgtcgtg catgagacat
      541 tgcgccatcc gttccgccgc actggcaatc ggcgccgcca gcgcgtccat cgcctgaagt
      601 ttcaagtgcg cgctgtcgga gaagtggccg ttgattctgg cgattaaatc catttatttt
      661 ccgttatcgc aagtttcaaa gtccgaaaca gcgtctattg tgcggcatat cacggttatt
      721 cttcaaatgc gtttttaatc cattcgatct cgcgcccgcc ggtctcgctc aacaggactg
      781 catcgaaacg gcaggccggg ggcgttttaa gcgacgacaa gtaatgccgc gccgtcttga
      841 ccagccgcgc ctgcttttgc gccgtaatgc ttgccgcggc gcccccgaaa ttctggttct
      901 tgcgcagtct gacttcggcg aacaccagcg tgtcaccatc gcgcattatc aggtctattt
      961 caccgaagcg gcagcggtaa ttagtttcaa gaagcttgag accttgcttt tgcagaaagg
     1021 ttgcggcatg tttttccgcc gcgacaccat cagcgcttcg gttcatagag gacgaccgct
     1081 tggccttgat agaacatagc ggggaggagt tcgcgcacga attgattctc ggcgttcaag
     1141 gtaatttgtc ccgttacccc gtcgagagta taacccaggc ccgggcgtgc attgagcaga
     1201 tcctgcgcga tgcggtaagc atcaatcccc agtgcgtaga ggcgctcgag gtccacaatc
     1261 accggacgat tcgggcgcgg gtaaatcatg acggccggat ggtcggattg caaaacccag
     1321 ggcatgtcca cgaagcggat accgttcaaa tcgaaattga ggggcccctg ggtgttgccg
     1381 ctgaaaatct gcgacgtggc gtagatattg gcgttggcgt caagatacgg ccgcaccagc
     1441 cgcgcacgtc tggcgtccag cgccagaaaa atcgtatccg ccccgctgat ggtcacggcc
     1501 tgcctgagtg tttgcaggcc tcccgggtcg ctgatatata ccaattgctg caggatcacg
     1561 ccgccgagtt tttgccacgc atcggcgaaa gcttgctgca tgcgctggct taatggcgta
     1621 tcagtggtga tgatgagcgc cttgcgccgg ccttcctggg cggcgagaga cgcagcctgc
     1681 tgcgcttcat tttccacatg cagactgagg gtgaacagat ttgcgggcag cgccgtgaag
     1741 tcatcgggaa gattgagggc aagcgtaggc acgcgcacca tgccgcttct ggccagcgcg
     1801 gacacgccgt cgcgcgtgag cggccctacc acgacttgcg cttccgccgc cagcgccagc
     1861 tggtaggcgg agagaatttc atcgatttgc tcggtagtcg cgtaggtttt gatgggcagc
     1921 gacgtattgc cctgatggct ggcggcggcg agaaaaccct ggcgcgtgag ttccgcggca
     1981 ttgccgaacc cttcggattt gagcggcaga atcagcgcga tgtgcggctt gggtggattg
     2041 gtctggagat tggcttccgg ttttgtcgcg gtcacagctt gcgccggtaa tggcttggct
     2101 gcggccgggg gcgcaatgct ttgcgcgcgg gtggcaaagg cgacggtggc accgtatagt
     2161 agcgccaaca gcgtcagcag aaaggccggg aaacgttgca tcgaggacaa aacgacaaag
     2221 gggagcccct attatatgta gtcgccaccc cgattggaaa tttgcaggac atcagcctgc
     2281 gcgcgctgga cgtactgaag cgggttgatg tggtggcggc cgaagatacg cgcaacactt
     2341 cgcgtctgct caagcgtttt gcgattgcag tgaagctcat cgcacttcat gagcacaatg
     2401 agaaaagcgc ggcggccagg atcgttgcgc ttttgcgatc gggtaaattc gtagcggtgg
     2461 tgagcgatgc cgggacgccg gcaatcagcg atcccggagc gctggtggtg gagcgagtgc
     2521 gagtcgcggg ttttcgcgtg gtgcccgtgc cgggtgcgaa tgccgcagtc accgcacttt
     2581 ccgcagcggg gctcgaatcg tcgcatttcc tgttctacgg gttcctgccg agcaaacctt
     2641 ctgcgcgttt gcaagtgttg cagaatatca aaacgttgcc ctatacgctg gtgttttatg
     2701 aggcgccgca tcgcatcatt gaatgtgtaa gcgacatgca acaggcattg ggcggcgagc
     2761 ggcgtgtcgt catcgcgcgc gagcttacca agctcttcga gaccatccat agctgtacgc
     2821 ttggcgaggc ggcagagtgg ctcaatgctg acccaaaccg gctcaaaggc gaattcgtgc
     2881 ttttggtttc cggcgtcgag cttgttcctg ccagtacaaa tatcacggac gcagagcgga
     2941 tcttgaagct attgcttgcc gaattgccgc tcaagcgggc ggtgaagctc accgccggaa
     3001 tcagtggcgc aaacaaaaaa acactctacg cactggcgct gcgaattaag ggtacagcaa
     3061 agcaggtata atggcgcgaa gatggccttg caaaaaataa ccatcacccg ccccgacgac
     3121 tggcatgtgc atttgcgcga tggcgcacca atgcgtgcgg tgcttgccga taccgcgcga
     3181 cgtttcggac gtgctattgt catgcccaac ctcaagccgc cggtcaccac gactgaggct
     3241 gcgctggcgt atcgcaagcg gattctcgcc gcgttgccca agagcgtaaa gtttgtgccg
     3301 ttgatgaccc tgtacctcac cgacaaaacc tctccgcagc aaatcatcaa ggccagacac
     3361 agcagcatcg tccacgccgc gaaatattat cctgccgggg ccaccacgca ttccggtgcg
     3421 ggtgtgaccg atatcaagaa atgttctgcc gcgctggagg agatgcaaaa gcaggatctg
     3481 ccgttgctgg tacacggcga agtagtggat tccgaggtgg acgtgtttga tcgcgagagg
     3541 gtttttatcg agcgtgtgct gacgccgctg ctcctgcgct ttccaaaatt gcgcattgta
     3601 ttcgagcaca tcacgactcg cgaagcggtc gagtttgtca gaagcactcc cgcaaatatc
     3661 ggagccacga tcaccgccca ccatttgctt ttaaaccgcg gcggaatctt ttccggcggc
     3721 atccgcccgc atcactattg cctgccggtg ttaaagcgtg aaaaccaccg tcttgcgctg
     3781 gttgaagccg caatcggcgg cagcccgaag tttttcctcg gcacggacag tgccccgcat
     3841 gcccaaagcg ccaaggaatc cgcctgtggt tgcgcgggaa tctacaccgc gcatgcggcg
     3901 atcgagcttt atgcggaagt gtttgagcgg gcgaacgctc tcgacaaact ggaagggttc
     3961 gccagtttcc acggcgcgga tttttaccgg ctgccgcgca atacggacac catcacgctc
     4021 gtcaaacaaa gcaaacgcgt gcctaagagc atcaaattcg gcaaggatag ccttgtccct
     4081 ttgcgcgcgg gtgaagaggt aagctggaga atgctttagc caggccgctt ctgctgaaaa
     4141 tgaaagcccc gcataagaag cggggcttat actgcaacgg ccacttaggc ccagcgccgt
     4201 tatcgcagcg ctctcaagta atcaataatt ttccggcttc acggaaatgc aggtatggtc
     4261 ggttccaaac cccccggcgc cgcttcggga tggcaccttt caacgagttg agaaatttgc
     4321 tccactttgc gcgcgggcac atccaccatt attaggattt tgccttcctc gatgtccttg
     4381 gcgaaaagca ggagcttcga attcggcacc gcgctggcga ctaaactcga cacccaggca
     4441 ccaagcaatg cgccgatcaa tgacgcgatc aacaccgtta ccaattgcaa ggttacgcct
     4501 tcaggcggag aaaataccat gatgatgcct agcgctgcgc cgccaactcc accgagggtc
     4561 atccccagtt ccgcgccgtg cacgatgtcg gttttttgca aaatgttggc ttcatgcaga
     4621 tcatcgagcg gtgtgccgcg ccgggcgagg cagtggatgt ggcgttcttc gatgcgcgcc
     4681 agtagcaggt cattcatggt ctgcttggcg ctggcgaggt ccggcaacac gaaatacagc
     4741 cttcgtctca tttcaacctc ttttcaggtc gcaatgctca agacttgtat gttggagcgc
     4801 tcgaaacaca cgaaagttgc atgcttgtgc cccccttaat ctggctttag ttcaagccgg
     4861 agcttaagtc aagtgataca attaaattgg aagctggcca gacggtcgct gtgcgcgctt
     4921 gcgcgcatgg aggaaagtcc gggctccaga gagcaggatg ccggctaacc gccggacgcc
     4981 gtgaggcgat gaacagggcc acagagacga gtcgcagtag acgcgcacaa acacgcgcac
     5041 cgctgcgggt gaaacgcggt aacctccatc cggagcaaga ccaagtaggc aagcgttgac
     5101 gttgctcgcc gagcttgcgg gtaggtcgct tgagcgtgtg agcaatccca cgtccagagg
     5161 aatggctgtc atggggtgca agccctaaac agaacccggc ttatcggcca gcttctttac
     5221 gtttcatgtt gcgcgcgcaa aagtttttct tgcgctgcaa tgtcattagg ctgttcccca
     5281 gtctttttaa aaccctatcc cgttattttt aaaagaataa tttacgctgt tgtaggcgtt
     5341 gtcgtcataa tgcgacttaa gtctttgtca caaataaaga aaagtcccgt tttaggcttg
     5401 acccgctgat tttttttatc tatagtgggg gttagtggtg aaaagtgggg aaaatttccc
     5461 aactaaaagg ggacagcact tgtttcgcgg cgtggcacaa ctcagtcttg ataataaagg
     5521 acggctcgcg attcccacca aataccggga gggactggct acgcgcggca acgggcgttt
     5581 ggtgattacc gccgatccgg atgcgtgctt gctcctctac gcccagcccg actgggaacc
     5641 catccagcaa aaactcaaca gcctttccag tttcaatccc aagctcagga atctgcagcg
     5701 cgtcatcgtg ggttatgccg aagacatcga gatggatgga acggggcgca tcctgatttc
     5761 accatcgctc agggaattcg ccgcgttcga caagcgcgtg gtgctggtgg gccaaggcat
     5821 gaaattcgaa ttgtgggacg cggagaaatg gaacgagcag cgcaatctgg cgcaatcgtt
     5881 tcgcaaccag ccgttgccgc cggagctgga aggattttcg ctgtaagcgt gagctcacag
     5941 cacgcaactg ttctgttgaa cgaggcagtt gaagcgttga aggtgcggcc aaatggcgtg
     6001 tatgtggatt gcacattcgg gcgcggcggc cacagccgcc tgttattgga gatgctggga
     6061 aaaaacggcc gtctggtcgc gctggataag gacccggcgg cggttaaggc ggcgcgcacg
     6121 ctgaaagatg cgcgtttttg catagtgcat cggcgctttg ctgcgttgcg cgaggtactg
     6181 tacgagctgg gcatcaagca tgtggacggt gtgctgatgg atttgggctt gtcctcaccg
     6241 caactggaag acgcggcacg aggtttcagt ttccggcgcg acgggccgct tgatatgcgc
     6301 atggacataa gccgcggtga aacggcggcg gagtggcttg agcgggcgaa tgaagaggag
     6361 atcggggagg tcataaaaaa atatggcgaa gaacggtttg ctaaacagat tgcaagagcg
     6421 attgttgcgg ctcgaaaggc aggacctgtt gataccacac ggcaacttgc ccagatcgtg
     6481 gcagcggccg tgcgcacgcg cgagccaaac caggacccgg cgacgcgcac gtttcaggct
     6541 ttacggattt acctcaatca ggagcttgag gaattgtcgc tagtgctgcc gcaaacggtg
     6601 gacgcgctca agcccggcgg acggctggta gtaatcagct tccattcgct ggaagaccgg
     6661 atggtgaagc gcttcatgcg cgatgaggcg cgcgcggacg atttgccggc caggttgccg
     6721 ctgcggacga acgagatgcg cagcccgaaa ttgcacgtga tcggcaaaac cgtgcggccg
     6781 ggcgtgcggg aaatcgcggc caacccgcgt gcgcgaagcg cagtgatgcg agtagcggaa
     6841 cgtaccgagg aggtatgaga aaaaagcggg cgagatacag acgtgactta cgaatgatca
     6901 agggtatgcc atgacgcggc tcaatctgat tttggtgctg attttgattg gctgcgcgct
     6961 gggtgttgtg acctcacagc acaaggcgcg caagctgttt gtggaactgc agaaagaaca
     7021 ggaatgggca cagcagctgg aagtggaatg ggggcaattg caactcgagc agagcacctg
     7081 ggcgacgcac gcgcgcatcg aaagaatcgc ggggaaaagc ctgcaaatgc atgtacccga
     7141 cgccgcgcgc acccagctgg tgaccccgcc ggaggagaaa gcgcatgaat agtcttgcca
     7201 atcccgtgct cacgctgcgg ttcccagtgt ggcgctcgcg ggtggtgctg ctggcgctcc
     7261 tgctgtggtt tatcgggctg ggcgcgcgtg ccgtgtatct gcagggcatg cacaacgatt
     7321 tcctgcagca gaagggcgat gcgcgctaca gccgcgtgat tgaaatcagc gcgcaccgcg
     7381 gcatgatcac cgacaggaac ggcgagccgt ttgccatcag cacgccggtg gaatcggtat
     7441 gggcgagccc cgccgacgtg gaaatcacgc cggagaaact gaagcagctc gccggccttt
     7501 tggaatcgga caagacggaa gtaggcaagc ggctcaagga gagcaacaag gagtttgtct
     7561 accttaaacg acagatttct ccggaacttg cagccagagt ggtgcagctc ggcatacccg
     7621 gagtattttt gcagcgcgaa taccaccgct attatccggc gggcgaagtg atggcgcatc
     7681 ttttgggttt caccggcatg gacgacaacg gccaggaagg cctggaactc gcctaccagg
     7741 attggctcgc cggcaaaccc ggcagccggc gcgttatcaa ggaccgcctg ggacgcatcg
     7801 tcgaggacgt ggagagtatc agtgcgccac aggaagggcg cacgctcact ttgagcattg
     7861 acagcaagat tcagtatctc gcctatcgcg aactcaagca ggcagtggaa gcaaaccacg
     7921 ccaaagccgg cggcatcgtg gtgctggatg cgaagagcgg cgaagtgctg gcgctcgcca
     7981 atgtgccttc gtacaacccc aacaaccgca tcaggctcaa tcttcagcgc acgcgcaacc
     8041 gcgcgataac cgatttgttc gagcccggct ccacattgaa accttttacc atcgccgcgg
     8101 cgctggaaag cgggcagtac acgccggaga gcatgatcga aatcgcaccg ggacaatttt
     8161 ccgtcggcaa cgccaccatt cacgacacgc accgtgaagc ggtgctcaca gtggcgcaaa
     8221 tcatccagaa atccagcaac atcggtgcgg cgaaaatcgc gctctcgctc agttcgcaca
     8281 cgctgtggaa cctgttcagc cgcgtcggct ttggcgtggt cccgcattcc ggctttccag
     8341 gcgaggtatc cggaaaactc cgaccttaca aaagctggcg gccgattgag caggcgacca
     8401 tgtcttacgg ccatggcatt tcagtgagtc tgttgcagct tgcacgcgcc tacaccgtgt
     8461 ttgccgctga cggcgaattg aaatcgctgt cgttggcgaa actcgatgcg ccgcaaagcg
     8521 gcggccaagt gatttcaccc gccaccgcgc gcgcggtgcg tgacatgctg gaaatggtgg
     8581 tggaacccgg cggcacggcg ccgcgtgcgc aaattgtggg ttaccgagtc gccggcaaaa
     8641 ccggcaccgc gcacaaactg gaaaacggca gctacacgac cgatcgttat gtatcctcgt
     8701 ttgtcggcat ggcaccggca tccgacccgc gtcttattgt agcggtgatg ctggatgagc
     8761 cttccgccgg ccaatactac ggcggcaggg tggcggcgcc ggtgttcagc caggtaatgg
     8821 cggggagctt gcgcatgctg ggagtcgcgc ccgatgcgcc aaccaataac gtagtgctgc
     8881 ctgacgattt ccccgaaatc aaggaagaag tatgacgaga agatccgcaa gcagaaaagt
     8941 tgcagcaaaa ccaggcgtgc cgcgttttga cgcgcgagtg ctgaatcgcc ttggcgtcaa
     9001 aacgaaacgg ctggccaccg atagccggca aatcaagccc ggtgatacgt tcatcgccta
     9061 tcccggcgaa aaacttgatg gtcgcaatta catcccgcgg gcgattgccg cgggcgccgg
     9121 cgccgtgctg tgggagaagc gtgatttcaa atggaacccg caatggaaag ccgccaatct
     9181 gggtgtgaat ggcttgcgag aaaaagccag tgtcatcgcg aaccggattt atggcagacc
     9241 ttcgcacaag ctgtgggtaa taggcgtgac cggcaccaac ggcaagactt catgcagcca
     9301 ctggattgct cagtgcctga cccgcctggg caagaagacc gcagtggtgg gcaccctcgg
     9361 caacggtttc cccggccggc ttgagcccac cgtcaatacc acgccggacg cggtgctgct
     9421 gcaggagctt ctcgccggct tcttgcagcg cggcgcgcaa tgcgtggtga ttgaagtctc
     9481 ctcgcacagc ctcacgcagg gacgggtgaa cggcgtggcg ttcgacgtcg cggtgtttac
     9541 caatctctcg cgcgaccatc tcgattatca cggcagcatg caagcctatg ccgcggccaa
     9601 ggcgaaactc ttccactggc ccggcttgcg ctgcgccatc gtcaacctcg acgaccgttt
     9661 tgggcgggaa ctcgcgcggc agctcgaaaa acgcagagtc aaagtgctgg gttatggatt
     9721 tggcaagggc gatatcgccg ggcaccgcct ggacttggcg cgccgcggct tgtctctgga
     9781 aatcaggacg cgttggggcg gcgcgcgatt gcaaagccgg atgctgggca agttcaacgc
     9841 cgccaatctg ctggcggtat tgggcgtgct cctggtgagc ggcgtcaagc tcgaaccggc
     9901 cgtgcgtgcg ctgcaggaaa taaaaccgcc ttccggccgc ctgcaaatag ttaaagatca
     9961 gcctctggtg gtggtggatt acgcacacac cccggatgcg ctgaaaaaag tcctgcaaag
    10021 cttgcgttct attgcgcagg aaagcagcgg caagctcgtc tgcgtgttcg gctgcggcgg
    10081 cgaccgcgac cgcggcaagc gtccgcagat gggcagagtg gcagtgagcc tggccgatca
    10141 agtggtggtg accagcgaca acccgcgcag cgaagacccg ctcgccatca tcgccgacat
    10201 tgcgcgcggc tggctgcctg gatttaaaga gccggagcgt gcgccagtgg tcgagccgga
    10261 tcgcgccaaa gcgatacggc tcgcgattag cggcgcggcg gaaaacgatg tggtgctgat
    10321 tgccggcaaa ggtcatgagg actaccagga gattggtggc gagaaaatgc cgttttcgga
    10381 cgtacggacg gcgcagacag tcttgcagga aagcgccgga tgatgagcct gagcgaggcc
    10441 gcacagggaa ttggagcgct catgcgggga aacgatgtac gtttcactgc agtaagcacc
    10501 gacagccgca aagcagcgac cggcgatttg ttcgtcgcgc tgcgcggcga acactatgac
    10561 ggccacgatt acatcaacgc cgcgcgcgga cagggagccg ttgcggtgat ggtggataaa
    10621 gcggagcgcg ccgaaaactg ccccgccctg gtggtggaag acacgcggca aggattggtg
    10681 ggactcgcct gttactggcg ctccaaattt agtttgcggc ttgccgcgat tactggcagc
    10741 aacggcaaga ccacagtgaa agaaatgctt gctgccatct tgagaaaaca cgccggagca
    10801 gacgcggtgc tggcgacgca aggcaatctc aacaacgaca tcggcgtgcc acttacgctg
    10861 ctcgggctgc ggccaccgca ccgctacgcg gtggtggaaa tggggatgaa ccatcctggc
    10921 gaaatcgcta atctcgcgcg aatcgcgaaa ccggatgtgg cgctggtgaa caacgccggg
    10981 ccggcgcatc tgctgggatt aggttcggtg gaggcggtgg cgcgcgccaa gggcgaaatt
    11041 ttcgaggggc tgccggataa tggagtggcg gtgattaacg ctgatgacgc gcatgcgaat
    11101 ctgtggcgcc agttgtgtca gggccggcgc gtgctggatt tcggtttcga gcatccggca
    11161 gcaatcaaag ggcgctgcga gttgaaaaac gacagcagcg aaatcgcgct gcaaaccgat
    11221 gccggcggaa tttatctgaa actgcaagtg ccgggcctgc acaatgcgcg caacgcactg
    11281 gccgctgctg cttgcgcgcg ggctctcggg gtgcagcgtg aagcgattgc cgcagggctc
    11341 gcggaattcg ccggagtgaa agaccggcag cagcgcaagg ccgggaaaaa aggcgcgata
    11401 gtgatcgacg attcctacaa cgctaatccg gattcggtta aagcggcaat tgacatgctt
    11461 gcgggtttat cgggcatgcg cgtcctggtg ctgggcgaca tgggcgagct cggggaacaa
    11521 gcgccacagt tccatgcgcg aatcggcgcg caggcgaaag ccgccggcat cgataggttg
    11581 tacgcgctgg gcgcgctttc cgcggaagcc gcaaggcaat ttggcgaagg agccggtcat
    11641 tttacgcaaa taaatgattt gattagcgag gtggaaaaat ggatggcgcc gaacgtcacg
    11701 gtgctggtga agggttcgcg cttcatgcag atggaacgcg tggtggagcg gattgttgcc
    11761 ggccaggagg cggggcactg atgctgctcg ctctcgcaca gtggctggcg caggacatcc
    11821 gcactttcaa tgtgttcaat tacatcacct tgcgcgcggt tctggcgacg cttaccgcgc
    11881 tgctgatttc gttcatggtc ggcccggcca tgatcagaaa gctcgccgtg tacaaaatcg
    11941 ggcaggcggt gcgtgacgac ggtcccaagt cgcattttgc caaggccggc acgccgacca
    12001 tgggcggcgc gctgattctg gtttccatcg gattcaccac tttgctgtgg gcggatttgc
    12061 gcaaccgctt cgtgtgggtg gtgctggtgg tgactatggg tttcggtctg atcggctggg
    12121 tggatgatta ccgcaaagtg gtgcggcgca atcccaaggg cctgtcgccg cgcaccaaat
    12181 ttttctggca gtcggtcatc ggttttgcgg cggcgctgtt tctcgctttc agcaccacgc
    12241 tgccggcgca gaccaccttt atcgtgccgt tcttcaagca cgtggtctat cccctcggaa
    12301 ttttcggctt cgtcggcatg acttatttcg tcatcgtggg ctcgagcaac gcggtgaatc
    12361 tcaccgacgg attggacggt cttgccatca tgccaacggt catggtgggc agtgcgctcg
    12421 gcgtgttcgc ttatgtcgcc ggccacgcgg tgttttccaa atatctcggt tttccctaca
    12481 tacccggcgc gggagagctc acggtatttt gcgcggctct ggcgggcgca ggcctcgctt
    12541 ttctgtggtt caacgcctat ccggccgagg tgttcatggg cgatgtcggc gcactcgcat
    12601 tgggcgcggc gctcggcact atcgccgtca ttgtgcgcca ggaaatcgtg ctgttcatca
    12661 tgggcggcgt gtttgtggtc gagacactgt cggtgatgct gcaagtggcg tcgttcaggc
    12721 tcaccggaaa acgcattttc cgcatggcgc ccttgcacca tcactacgaa ttgaaaggat
    12781 ggaaggagac acaggtggtg gtccggtttt ggatcatcac catgatgctg gttctggtcg
    12841 ggttgtcgac attgaagctg agataaatgg aaatagaact taaaggcaaa aaagcgctgg
    12901 tggtggggct gggtgacacc ggactttcgc tcgcgcgctg gctcacgcgc aagggcgcga
    12961 ccgtgcgcgt ggtcgacagc cgcgaagcgc cgccgcgcgc agcgcaattg aaagtggaac
    13021 tgccgcagct tgcgctggat accggaccgt ttcatgaaga aagtttccgc gacattgaca
    13081 tgattgccct aagccccggc gttgccctcc gggagcctcg gatttccgag gctcttaacg
    13141 ccggggtctt tgtttgcggc gatgtggagt tattcgcgca ggcgctgaaa aaaatgccgc
    13201 tgaccagggt gctggcgatt accggctcca acggcaagag cacggtgacc agcatgacgg
    13261 gcgaaatgtg ccgcgccgcc ggactcaata cagtggtggc aggcaacatc ggcctgccgg
    13321 tgctggatgc gctcactcag gcggaaaacc aagtgcaacg ggggaaaagg ccggacgtgt
    13381 tcgtgctgga gttgtcgagc tttcaattgg aaacgactta taccttggag cccggcgcgg
    13441 cggcggtgct gaatctgagc gaagaccagc ttgaccgcta tccaagcatg gccgaatacg
    13501 ccgcggccaa ggcgcgcatt ttcgcgggca gcggcgtgca agtgcttaac cgcgacgatc
    13561 gccagagttt caccatggcg ggcaaggaac gccgtgtttt cagctttggc ctgggccggc
    13621 cgcttagaga aatggaatgg ggactgctcg aagtagcggg tgaaaaatgg ctggcgcagg
    13681 gcgaaatgaa gctcatgccg gtcaaggaat tgccgcttac cggtttgcat aatgcggcga
    13741 acgcgctggc tgcgctcgcg ctgtgccggg cgatcggcat cgagtatgcg ccgcttctgc
    13801 atgcgctgaa aaaattcagg ggattgccgc accgcgttca gaaaatcgcc gatatcggcg
    13861 gcgtggggtt ttacgacgat tccaaaggca ccaatgtcgg cgccacggtg gcggcgctga
    13921 acggcatgca gcaaaaagtg gtgctgattg ccggcggcga cggtaagggc caggattttt
    13981 cgccgcttaa aaccgccatc accgggcatg cgcgcgcttt ggttttaatc ggccgcgatg
    14041 gcgaaaaaat cgcgcaagcg gtcgccggcg gcaatgtgcc gatgcggcgc gcgaccagca
    14101 tgcgggaagc agttgccttg agttttagcc tggcgcagcg cggcgatgcg gtgctgcttt
    14161 cgcccgcatg cgcaagcttc gacatgtttc gcaattacga gcaccgcgcc gaagcgtttg
    14221 cgcaagccgt gcgcgaactg caaaaacagc cgcgcgtggg agcgaactga gtgttctacg
    14281 ccgcaccagc tggccgcgcg ccgctacctg cctacgatca gtcgctgctg ggggcgacgc
    14341 tggcgctgct cgggctggga ctggtgatgg tgtattcggc ctcgattgcc attgccgaag
    14401 cggggcgctg gcatcaaccg gcatatttcc tgattcgtca caccgtgttt ctgggaatca
    14461 gcgtcgtgct cggcgcgatc gcgttccaat ttcccatgta tttatggcag cgtgtctcgc
    14521 cctggctgtt cgtgctgggc gccggcctgc tcgtgctggt cctcattccc ggcgtgggac
    14581 gcgaagtgaa cggcagccag cgctggctgc cgctggggct cgtccacctg cagccttccg
    14641 aatttatgaa gctcatcgtg gtgatatatg ccgcggacta caccgtgcgc aaggcggcct
    14701 acatgcacag cttccgcaag ggttttctgc cgatgctgat ggtgatgctg ttgatcggtg
    14761 gattgctctt gcgcgaaccg gatttcggcg cgtttgcggt gatcaccacc atcgccatgg
    14821 cgattctgtt tttgggcggc atgaactggc ggctgttcgc cctgctcatc gtgctgctga
    14881 tggtcggctt cctgatcctg atctggactt cgccctaccg catgcagcgc atcatcggct
    14941 tcatggatcc ctggtccgat ccgttcggca aaggctatca actttcgcac gccctgatcg
    15001 ctttcgggcg aggcgaatgg ttgggagtgg gtttgggtgg cagcgtggaa aaactgtttt
    15061 atctcccgga agcgcatacc gattttctgc tggcggtggt tgcggaagaa ctcggatttg
    15121 ccggcgtggc ggcggtgatt gcgcttttta cctggctggt ggcgcgcgcg tttgcgatcg
    15181 gccgccaggc ggcgtatctc gagcgctatt tttcggcgct ggtggcacag ggcgtgggcg
    15241 tgtggctggg tgtgcaggcg gtcatcaaca tcggcgtcaa tatgggcgtc ctgccgacca
    15301 agggtttgac cctgcctttg atgagtttcg gcggcagcgg catcgccgca aactgtgtcg
    15361 cgatcgcctt gctactgaga gttgattggg agaacagaca gttgaagaaa gggaagccat
    15421 tatagatggg ccgcacgatc ttgattatgg cgggaggcac gggcggccac gtgtttcccg
    15481 gtttggcggt ggcggagtat ttcaaaaaac agggatggcg cgtggtgtgg ctgggaacgc
    15541 gcgatggcat ggaggcgaaa ctagtgccgc aacagggtta tgacatggcg tgggtgaact
    15601 tctccggcgt aaggggcaag gacgttttgc gcctggtgtt gctgccgctg cagctgatta
    15661 ttgctttctg gcagagcgcg caggcgatat tcaggcatcg ccctgaggtg gtcttgggac
    15721 tcggcggcta ccccgctttc cccggcggca tgatggctgt gctgttgaac aagccgctgg
    15781 caatacacga acagaattcg atcgcagggt tgaccaacaa agtgctggcg aaactcgccg
    15841 acaaggtgct ggtggcattt cccgaggcat tcggggcggc ggaaccgcgc aaccaaatct
    15901 ggaccggcaa cccggtgcgc gaagcgattg cgcaattagc gccgccgcag atgcgctatg
    15961 ccggccgcac gggaccgctg cggcttctgg tggtgggtgg aagcctgggc gcgcaggcat
    16021 tgaatgaaaa cgtgccgcaa gcgctcgcgc tcttgccaaa agatgagcgg ccactcgtta
    16081 ctcaccaatc aggcgcccag catgttgacg cgctcaagca agcgtaccgg cgtgccgccc
    16141 tcgaggtgcg catcgaagcg tttatcgaca acatggcgca gtgctacgcc gaatcggatt
    16201 tggtaatttg ccgcgccggt gcgctcacca ttgccgaaat cgccgcggca ggcgtggcca
    16261 gcattctggt tccctatccg cacgcggtgg atgaccatca gactcacaac gcgcgctttc
    16321 ttgccgatca aggcgcggca atgttgttgc cgcaaacaga attgaacccg aattcgcttg
    16381 ccggtctgat caaaagcttg tcgcggggaa aactgctggc catggccagc cgcgcccgcg
    16441 aactcggcaa gccggacgcc acccgcaaag tggcggaaat ctgcatggag ctcgcgagat
    16501 gattgagagc attaaccgca gaggcgccga gacgcggaga aaatcaaaac tttttacgtc
    16561 tttatgtttt gttatcgaag tgaaaatgaa tagtctgtgc cggagcattt caagcgcatg
    16621 gaaatttaca tggcaaactc tgcgcctccg cggtaaaggt tctttatgcg gcataaggtg
    16681 aagcgcgtgc attttgtcgg catcggcggc tcgggcatga gcgggatcgc cgaagtgctg
    16741 ctcaatctcg gctttgtggt gagcggctcg gacctttccg aaaatgccgt gacgcaacgg
    16801 ctaaaaagcc tgggcgcgac ggtttacaag gggcacgatg cagcgcatat cgagcgcgcc
    16861 gacgcagtgg tgttttccac cgcggtgaaa gctgacaatc cggaggtgac agcagcgagg
    16921 aatcacaaga tcccggtagt accgcgcgcc atgatgctgg ccgagctgat gcgcctgcgt
    16981 caaggcattg ccgtagccgg cacgcacggc aagaccacga cgaccagcct gattgcgagc
    17041 gtgctggcgg aagcagggat ggatccgact tttgtaattg gcggcaggct cgaagccgcc
    17101 ggctcgcacg ccaaactcgg cggcggcgag ttcatcgtgg tggaagcgga tgaatccgac
    17161 gcttcgtttc tttacctgca gccggtgctg gcggtggtga ccaacatcga tgccgaccac
    17221 atggaaacct acgcgcatga tttccggaag ctcacccagg cctttgtcga ttttctccag
    17281 catttgccgt tttatggcac cgcggtgttg tgcagcgacg acgcgcgggt gcgcgaaatc
    17341 atcccgcacc tgaccaagcc ggtcactacc tatggcttgt cgcaaagcgc gcaaatccgc
    17401 gcggtggacg tcaagcacca cggcggacag atgcagttcc gggtggtgcg caccagcggc
    17461 aaggatctgc agatcacgct taacatgccg ggtgtgcaca acgtgcaaaa cgccctggcg
    17521 gcgattgccg tcggcatcga actgggcgta gctgattcgg cgatggccaa ggcgctggcg
    17581 gagttcaagg gcgtggggcg gcgtttccag cgttacggtg aagttccact accacgtggc
    17641 ggtaacttca ccctgatcga cgattatggc catcatccgg cggaaatggc ggcaacactg
    17701 gcggcggtgc gcggcgcgtt cccgggaaaa cgcgtggtgc tggcgttcca gccgcatcgc
    17761 tacacccgca cgcgcgacct gtttgaagac ttcgtgaaag tgctttccgc cgtaaatgtg
    17821 ctgctgctgt gcgaagtcta tccggcgggc gaggcggcga ttcccgcggc cgatggcaga
    17881 acgctggtgc gcgccatacg cgtgcacggg aaagtcgagc cgattttcgt ggaaaacatc
    17941 gccgagctgc cgcaggtgat attcaacgcg gcgcaagccg gcgatgtggt ggtgaccatg
    18001 ggcgcgggct cgattggcgg ggtgccgggc gtgctggcaa gaccaaccgt gagtagcagt
    18061 gcatgaacat ggcggaaacg aaccatctgg cgggcttgcg tggtgagctg cggttcaacg
    18121 aaccgatggc ggagcacgta agctggcgcg ccggcggcag ggccgcgcgc gcctacatcc
    18181 ccgcggatct tgccgatctc gtcgcgtttt tcgagacctg cggcaagaac gaaccggttt
    18241 acatagtggg actgggcagc aatcttctgg tgcgcgacgg cggcgtgcgc ggcacggtgg
    18301 tgttgctgca ttcggggctc aagcaattgc gcctcgaatc ccgggaagcg ggctgcgcaa
    18361 tgatttatgc cgaagccggc atggcgtgcc ccaaggtggc gcgctttgcc gcgacgcatg
    18421 acctgcatgg cgcggagttt ctcgccggca ttcccggcac cgtcggcggc gcactggcga
    18481 tgaacgccgg ttgctatggg tcggaaacct gggaaatcgt ggcgcaggtg ctgacactgc
    18541 gccgtgacgg aaccctgagt atgcgcactc cggcggacta ccgcatcggc taccgcagcg
    18601 tggcgccaca ttcgaaattg gaagagtggt ttgccgccgc ctggttccgg ttgccgcagg
    18661 gcgacagcaa agcggcgaaa gaaaaaatcc gcgatttgtt gaaacgccgc atcgccagcc
    18721 agccgctgga gctccccaac gccggctcgg tgtttcgcaa cccgcaaggc gtgtacgcgg
    18781 cgcggctcat cgagcaatgc ggcttgaaag ggttgcgctt cggcggtgcg gaagtatcgg
    18841 ccaagcacgc caatttcatc gtcaacaccg gcggcgcgac cgcggctgac atcgaggcgc
    18901 tgattcaaaa aattcagctc acggtagcgc aggagtgcgg cattaagctg gaaaccgaag
    18961 tgcggattat tggtgaagcg tgaaaggtaa aaagcttgga aaggttgcag tgctaatggg
    19021 tggccgctcc gctgagcggg aaatttcgtt caagagcggc acagctgtac ttaacgctct
    19081 ccacaagcgc cgggtcaacg cacacgcttt cgatccaagc gcacggcgac tggaggattt
    19141 acgaagcgga ggattcgagc gcgtatttat cgcactgcac ggcaggcagg gcgaagacgg
    19201 cacggtgcag ggcgcgctgc aactcatggg catcccgtac accgggagcg gggtgctggg
    19261 gagcgcgctg gcgatggaca aatggcgcac caaactggtt tggcgcgctg ccggagtcgg
    19321 cacgccgcgc tacgaattgc tggatgaaaa aagtgatttc aggaaaatcg cgaaaaaact
    19381 gggtttgccc atcatggtga agcccgtgtg cgaaggctcg agcatcggca tgagcaaggt
    19441 gatgaaagcg gagaaattac gcgccgcctg gcgcctggcg gcgaagtatg acccggtggt
    19501 catcgccgag caattcatca agggcgtgga attaaccgtt tcgattctcg acggcgaagc
    19561 gctgccgctg attcgtcttg agacgccgcg cactttttac gactatcaag cgaaatattt
    19621 ttcccgcacc acgcgctata ttctcccgtg tggtctttcc gcgccgcagg aaaaaaaaat
    19681 tcagcaaaaa gcgctgcacg cgttccgcat tctgggttgc agcggctggg gccgggtgga
    19741 cttgatgctc gacaagagag gacggccgta ttttctcgaa gccaataccg tgccggggat
    19801 gaccgaccac agcctggtgc cgatggcggc gcgcgccgcc ggcttgagtt tcgaggacct
    19861 ggtgttgcgc attctggagc aggcgcatgt ggaataaatc gtacgcgatg ctttggctcg
    19921 ccaatctgct gtacacgttg gcggggttgc tcgcgcttta ttgcgcgctt tatttcgcga
    19981 ttcggctgcc gtggtttgag cttaagaccg tacaggtaag aggcgaactc acgcatgcct
    20041 cgcaggagca gttgctggca gtggtgcgcg gcctcaaggg gaattttttc accgtggatt
    20101 tagacgaagc gcgcagccgc ttcgagaaat tgccgtgggt aagaggcgtg ggcctgcgcc
    20161 ggcaatggcc ggaccggctg gaggtcgcgc ttgaggagca ggtgccggta gcgcgctgga
    20221 gcaacggcgg gctggtgaac acctacggtg aagtgtatgc ggctgaagcc gatgcggatt
    20281 tgccactttt ttccggacct gctggaacca gtcaggacat cgccaaggct tacgccgcct
    20341 tcaagcagat gttggagccg atccagcgcg taccggtgca ggtgcgcatg acgacgcgcc
    20401 gcgcgtggca aattcagctg gatgacggat cgactctggc gctgggaagg gaacggatgg
    20461 acgcgcgctt gaagcgcttc gtcgcggcgt atgaccgcac tttgggcggg ctgcaatggc
    20521 aatcggatta tgttgatctg cgctatgaca acggttttgc gttgcgcgtg cctgaagcgg
    20581 ttagagagca gagtgagccc gccaaacggg aaaaacacaa ggcagcctga gaggatttac
    20641 agaggatggt aatgggcagg agcaaggaaa acaaggatct gatcgtcggc ctggatatcg
    20701 ggacgtctaa aattgccgcc atcgtcgccg aaatgcgccc ggaaggaggg ctggaaatca
    20761 tcggcatggg gagccacctc tcgcgcgggc tgaagaaagg cgtggtggtc aacatcgagt
    20821 ccacggtaaa cgccatccag cgcgcactcg aggaagcgga gctgatggcc gattgcaaga
    20881 tccgcgaggt gtacacggga attgccggca gccacatcaa gagtttcaac tcgcacggca
    20941 tggtcggcat caaggacaag gaagtgtcgc agatggacgt ggaccgggtg atcgagacgg
    21001 cgaaggcggt gaacatcccc accgaccagc agatcctgca catcctcaac caggaattca
    21061 tcatcgacgg gcaggaggac gtgcgcgagc ccctgggcat gagcggggtg cgcctggagg
    21121 tgaaagtgca catcgtcacc ggcgcagtct cggcggcgca gaacatcctg aagtgcgtgc
    21181 ggcgctgcgg gctttcggtg cgcgatctga tcctgcagcc gctggcttcc gcgatcgggg
    21241 tgctttccga ggacgaaaag gatttgggcg tgtgcctggc ggacattggc ggcggcaccg
    21301 ccgacatcgc ggtattccat cacggggcga ttcgccatac tgcggtgatt ccgattgccg
    21361 gcgatcaaat taccaacgat atcgcggtgg cgttgagaac gccgaccaag gacgcggaag
    21421 atatcaagcg ccgttacggc tgcgcgttgc ggcagcttgc cgatccgcag caaatggtgg
    21481 aggtgccggg cgtgggcgaa cgcggcccgc ggcagctttc aagacagacg ctcgccgaag
    21541 tgatcgagcc gcgggtggag gaactgtatt cgctggtgca gacggaactg cgccgcagcg
    21601 gcttcgagga gctgctttcc tccggcatcg tgattaccgg cggcagcagc gccatgcagg
    21661 gcatggtgga gctgggcgag gaagtgttcc acatgccggt gcgtctgggc ttgccgcatt
    21721 acctcggggg cctggtggaa gtggtgcgtc atccgcgctt cgccactggc gtcggcctgc
    21781 tgctgctggg catgcagcag cagcagcggc aggagctggc gaagctgcaa agcggctcgc
    21841 tcaagcaggt tttcgagcgc atgaagcgtt ggtttcaggg aaatttctga cggattttga
    21901 ttgggtaaac aggggaaaaa tgcaggaaca gatgaaattt gggattaacc gatggcggtt
    21961 ttacggaaac agcagctact tagctaaagg aggtcaacat gtttgaaatc gtggatagcg
    22021 aagcgaagga agcgcaaatc aaggtagtcg gtgtgggcgg ctgcggcggc aacgcggtag
    22081 accatatgat ctaccgcggc gtgaaaggcg tggagtttat cagcgccaac accgacgtgc
    22141 aggcgttgcg gcgcaaccag gcgaaaatcc agttacaact ggggaactcg attaccaagg
    22201 gcctgggtgc gggggcgaac cctgacatcg gacgcgaagc ggcgctggaa gaccgcgagc
    22261 gcatcaccga actgattcag ggggccgaca tgctgtttct caccgccggc atgggtggcg
    22321 gcaccggcac cggcgccgcc ccggtgatgg cggaaattgc cagggacctc ggcatcctca
    22381 ccgtggcggt agtgaccaag ccgttcgcct tcgaaggcaa gcgcatgggc atcgcgcggc
    22441 agggcatcga ggcgctctcg cagttcgtgg attcgctgat cgtggtgccg aacgacaagc
    22501 tgatgcaggt gctgggcaac ggggtcacgc tggatgcggc gttccaggcg gcgaatgacg
    22561 tgctgcacgg ggcggtggcg gggatcgccg aaatcatcag ttgcccgggc atgattaacg
    22621 tcgatttcgc cgacgtgcgg acggtgatgt cggaaatggg catggccatg atgggctcgg
    22681 ccaaagccgt cggcgccgac cgcgccaagc tcgccgcgga acaggcggtg gcctgcccgc
    22741 ttttggaaga catcaacatc ttggatgcgc gcggcgtgct ggtcaacatt tccgccagcc
    22801 gagacagctt ccagttgcag gagatgtacg acgtgatgga caccatcaag acctttgccg
    22861 cggaaagcgc gacggtgatt gtcggcgcgg tttacgatga agagctgaag gacagcctgc
    22921 gcgtcaccat cgtcgccacc ggcctgaacg gaccggtcaa ccgcatgcag gaaaagccgt
    22981 tgagcgtggt gaagaccggg acccacgatg cgccggtgga aatggtgaat tacgccgaac
    23041 tcgaaacgcc agcggtggtg agaaggcgca accgtgacgc caccatcgag gcgatgcgcc
    23101 agtccggcgt ggagatgctc gacatcccgg cgtttctgcg caaacaggcg gattgagccg
    23161 gcttaaacgg cgcaaattag ggggcggggg tagcttgtca caatcagtat gtgataggat
    23221 taactcttcc gcttcaagat gataacttac tagaacgcac tactcataaa ttatgataaa
    23281 gcagcgcacg ctgaaaaata ttattcgtgc gaccggtgtc ggcctgcaca ccggcgaaaa
    23341 agtctatctc accttgcgtc ccgccgcgcc taacaccggc atcgtgttcc gccgtatcga
    23401 tctcaagcaa cctgtggaaa tcaaggccga cccgcattcg gtcggtgaca ctcggctttc
    23461 ttcgtgcctg gaaaaaaacg gggtcagggt ggccacggtt gaacacctga tgtcggcgtt
    23521 cgccggcttg gggatcgaca atgcttatgt agacctcacc gcgccggaag tgccgatcat
    23581 ggacggctcg gcctcgcctt tcgtgttcct gctgcaatcg gccggcatcg aggagcagcg
    23641 cgtgccgaag gaattcatcc gcatcaaaaa gatggtcgag gtggtggacg gcgacaaatg
    23701 ggtgcgcttt gaaccgtaca acggcttccg cgtcaacttc agcatcgact tcgatcatcc
    23761 ggtgtttgaa ccgtccggcc agtcggtgac ggtggacttt gccaacacgt cctatataaa
    23821 agaagtcagc cgcgcccgca ccttcggctt catgcaggaa gtcgaaaccc tgcgctcgat
    23881 gggtctggct ttgggtggca gcctcgacaa tgccatcgtc atggacgaat accgtgtgct
    23941 caacagcgac ggcctgcgct acgaggacga gttcgtcaag cacaaggtgc tggacgcgat
    24001 tggcgacctg tacctgctag ggcatccgct gattggcgcg ttcaccggcc acaagtccgg
    24061 ccacacccag aacaacgcac tgctgcgcgc gctgctcgcc gatgcccagg cttgggagtt
    24121 cgtgagcttc gaaagcagcg aggaagcacc ggcgttcctc aggctgcaaa cgcagtttgc
    24181 ctgaggcttc attcctctgc gttgagttct cacgggaagc agttcaagtc cggccgcatt
    24241 ccggcggttc ccgtctcttc gaagtgccgc tatgcccgta gtcgtgctga gatttgtcgc
    24301 ctatatagcg ttgatcgcga tagggctagc gctggcgctg tggctgttca cccgaaaccg
    24361 ccgctacctc aatttcgcct ggcagttatt gaggtttttc gtggttttgg ccgtcttggt
    24421 cttgggactc tttgtcctgg aacggctgat catcatcatc tgagggccgc gccgccattt
    24481 tttccagcgc ggtccgcagg ggggtgatgg gaagactcgc cgcgagctca cgcaaatacc
    24541 cgctggcttc gcggcttaac ctgatgtttt tatgcggcac tgcggcacga cctgggggtt
    24601 gcgccgtaac ccgaattgca gtaacctcgt atccccgctt ctggaattta actaacaagc
    24661 taggcaccag ctgcttcagt tttgcggcga cagccccgtt gtccgcaagc agcagcagcg
    24721 acccgtgcgc aaaccggccc acccgtccgg agcgtgcgag gggggatggt gcaatctcgg
    24781 caaacacttt ttgcatttcg atcaggcgcg aagcctcttt aaccagtgac tgcatcgcag
    24841 ggttagtgtc gaaatagaaa ccgagtttgc gtacgggcat gcgaagccgg gcaaagtgag
    24901 ggagacaatg aatattattc tagtttccgg gaacctggga aagtccagaa caacaacact
    24961 tagccattcc cagatcgttt tcaccgcggt gctgctgctt atcggcatcg tgacgttcgc
    25021 cattgccctg cattacctga cgctgctccg cgcggcggaa atcaagagcc cttatctgca
    25081 agcgctgctg gtttcggtac aggagcagga actgcgcaag cagcaggcct atctgcgcga
    25141 aaatctcaac gccatggcgg tgaagctggg acagatgcag gcgcaattgc tgcggctcga
    25201 cgcgctcggc gaccgcctcg ccaagcaggc gggcttcaag ccgcaggaat tcatgtttga
    25261 ccagctgccc ggccgcggcg gcgcggtctc cggcactccg caacgcgatc tcaccctgaa
    25321 cgagttcaac cagcaaatgg aacgtctgtc cagcgagctc agcgaccgta ccgacaagct
    25381 gggcattctg gaaactgcgc tgatacaaga ccgcctgaag cagaaactgg tgccttcggt
    25441 gccgccggtg gacactggct ggttctcctc gaatttcggc tggcggatcg atcccttcac
    25501 cggcagcaat gccttccacg aaggcgtcga tttcatgtcc gaagtcggct ccacggtggt
    25561 ggccgccgcc ggcggcgtgg tggtgcattc ttcggacaac aactccgagt atggtagtat
    25621 gatcgagatt gaccacggca acggtttggt gacgcgctac gcccatctat ccaagcgcct
    25681 agtcaaggtg ggcgacgtgg tgttgcgcgg gggcaagatt ggtgaagtgg gaaacaccgg
    25741 ccgcgcgacc ggcccgcatc tccattttga agtgcgcttc aagggtgccc cgcaaaatcc
    25801 ggtgcggttt ctgcataagc ccggttgacg acagactgtg aagcgtaagg ggtgaggggc
    25861 gaggcaaaac tcctcgcccc ttgcgttttc taacggcaca tagttgcctt gtaatttcat
    25921 gctggccagc ctgctgaaaa aaatcttcgg cagccgcaac gaacggctgc ttaagcaata
    25981 cacgcacaca gtgcgggcga tcaacgcgct ggaacccgcc atcgaaaaac tttccgacgc
    26041 cgagctgcgc gccaagaccg acgagttcaa gggccgcatc cgcgagcggc tcgcgactat
    26101 tcccgaccat gccgatggcg acgccgagcg ggcgcaggcg caggaagaag agcgcagccg
    26161 cgcgcgcaag gcgattctcg aggaactgct acccgaagcg ttcgccgtgg tcagggaagc
    26221 gagcaaacgc accctgcaaa tgcgccattt cgatgtgcag ttgatcggcg gtatggtctt
    26281 gcattatgga aaaatcgcgg aaatgcgcac cggagagggc aagactttgg tggccacgct
    26341 gccggcgtat ctcaacgcgc tcaccggccg tggtgtgcag gtggtgacgg tcaacgatta
    26401 cctagcaagc cgcgatgccg aatggatggg cagaatctac aatttcctcg gccttacggt
    26461 gggggtcatt gtttcgcagc aggaggatgc cgccaaacgg gccgcctatg ccgctgacat
    26521 cacctacggc accaataatg aatttggctt cgactatctg cgcgacaaca tgaagtacgc
    26581 cgtcgaggaa cgcgcgcagc gcggcctgca ctacgccatt gttgacgagg tggattcaat
    26641 actgattgac gaagcgcgca ccccgctcat catttccggc caggccgatg acaccaccga
    26701 tttgtatcac cgcatcaacg acctggtgcc gaagctgacc cgccagcagc aggaaaacgg
    26761 gccgggcgat tacagcgtgg atgaaaaaac caaccaggtg ctgctcaccg agaccgggca
    26821 cgagcgcgcc gaacaattgt taagccaggc cgggctcctg ccgccgggcg gcagtcttta
    26881 tgactccgcg cacatcaatc tagtgcatca tttgtatgcc gcgttgaaag cccatgcgct
    26941 gtttcaccgc gaccagcact acgtggtgca aaacgccgaa gttgttattg tcgacgagtt
    27001 taccggccga ttgatggcgg ggcggcgctg gtcggacggt ctgcaccaag cggtggaagc
    27061 gaaggagggc gtgaagatcc agcgcgagaa ccagacgctc gcttccatca ctttccagaa
    27121 ttatttccgc atgtataaga agctcgccgg catgaccggc acggcggata ccgaagctta
    27181 cgaattccag caaatctacg gcctggaaac ggtggtgatc cccacccaca agccgatgat
    27241 ccgtgaagac cgtcaggacc aggtgtaccg caccaccaag gaaaaataca cggcggtcat
    27301 taacgacatc cgcgattgct gcgagcgcgg ccagccggtg ctggtcggta ctacctcgat
    27361 cgaaaactcg gaactacttt ccggtttgct ggatcgggaa aaactgccgc atcaagtgct
    27421 gaacgccaag cagcatgcgc gcgaggcgga aatcgtggtg caggccggac gaccgaaatg
    27481 gattaccatc gctaccaaca tggcgggacg cggtaccgac atcgtactcg gtggcaaccc
    27541 cgagccggaa atcaataaaa tccgcagcga cgcaaacctc gacgacgcct ccaaggcagc
    27601 gcgcattgag gaaattcgcg cgcaatggca gaagctgcac gaacaggtgg tggcggccgg
    27661 gggcctgcac atcatcggca ccgagcgcca tgaatcgcgg cgggtggaca accagttgcg
    27721 cggccgctcc ggtcgccagg gcgacccggg ctccagccgc ttttatctct cgctggaaga
    27781 tccgctgctc aggatttttg cttccgaccg cgtttccgcc atcatgcagc ggcttaacat
    27841 gcccgagggc gaggcgatcg agcatccgtg ggtcacgcgc gccattgaaa acgcacagcg
    27901 caaagtggaa gcgcgcaact tcgacatccg caaacagctg ctcgaatatg acgacgttgc
    27961 caatgaccag cggcgggtga tttaccagca gcgcagcgaa ctgctggaga gcactgacat
    28021 ttccgacacc atccgtgcca tgcgtgagga cgtggtgaac gatttaatca gccagcatat
    28081 cgcgccggaa agcgtggaag agcaatggga tgtctccggg ctggaaaaaa ccatcgccgt
    28141 ggaattgcag ctcaacctgc cgctgcaact ctggctcaag caggaccccg agctgcacga
    28201 ggaaacattg cgcagccgca tcatcgaagc ggcgcatgcg caataccagg gcaagctgga
    28261 gcaggtcggg accgaagtga tgcaccagta cgaacgcgcg gtaatgctgc aaagcttgga
    28321 ttcccactgg cgcgagcatc tggcggcgct cgatcatctg cgccaaggca tacacctgcg
    28381 cggctatgcg gcgaagaacc ccaagcagga atacaagcgc gaggcgttcg agcttttcgc
    28441 cggcatgctt gaattgatca aggctgaagt cacgaaaatc atcatgacgg tgcagatcag
    28501 gagccgggaa gaagtagagg cggtggaacc gccgccgcag ccggaaaatg tgcaatacca
    28561 tcatgctgat tacgacgaag cactggcgca ggccggcaac gccgagcaaa agccattcgt
    28621 gcgtcaggga cagaaaatcg ggcgcaacga tccctgcccg tgcggttcgg gcaggaagta
    28681 caagcactgc cacggcaagt tgagttgatc ccgcgctgcc cgatgtaatc gcaaagcgcc
    28741 gacaaggagg cggaaaatgc agacttggaa tttacacctg taccagttca tgaacgattg
    28801 gaggctgttc cgtcgcagtt atctactcaa gttcatgctg gtcgcgtttc tcggcaccca
    28861 tgtgcctctc atcgttctgg tgctgtatat tgcgctgtcg cagtcctaca gcctgatggg
    28921 cgcgctgccg atcgtgattg cgactctgtt cgccaccctc atcggcgccg ccatcacact
    28981 gttgtttctg ggcagtctgc tgctgccggt gcgccagact tcggagagcc tcaaacaata
    29041 tctggccgaa cgcaacctgc cgagtctccc caccatttac ggtgacgagg cggggcagct
    29101 gatggccgat acgcagtatg tcattatgcg tctcgacaat gcgctgcgcg agctcgaaaa
    29161 tatttcgctc accgatcatc tgaccggcgt ttacaaccgt cgcgccggcg agcagcgcct
    29221 caatgaggaa atcgcgcggg cggaacgcga gaacaagcat ttcctgctgg ccttcattga
    29281 tatcaattcg ttgaagaaga tcaacgatca atacggccat gatgcgggcg acgcctgcat
    29341 catccatgtc gccacgctgc tggccgaaaa catccgccgc ggtgactggt gcgcgcgctg
    29401 ggccggtgat aaattcatcc tggcggtgta tcaccccgat atcagtaccg agctggtgtt
    29461 caaccgcgtg ctggcgaagc ttcaagccaa gccttgcctg gtgcgtcata acgtcacgct
    29521 caatctcagc gtgagcatcg gggtggcggt gtaccagcgc ggcgttgcgg ttgaaacctt
    29581 ggttcagcag gctgatcagg cgatgtacca ggccaagcta ttaggccagg accagtcgca
    29641 tgtggccttt tgtggcaaat aaggcttgaa aattgcatag ggtttgaaaa tttcaaaagg
    29701 cagaatgagg aaaaatagat gccggtgaat ttgcactcac cccaacctga acaagtgctg
    29761 ccggtaaaag gcgttgccct cggcgtggcg gaggcggcaa tcaaaaaacc cgggtgcaag
    29821 gatttgctgg tgatggtgtt tgagccgggc gcaaaagtcg ccgggttgtt tacccaaaat
    29881 cgtttttgcg ccgccccggt gctgctggca aaaaaacact tgagttccgg tctgccggtt
    29941 gaggcgctgg tggtgaacac cggcaacgcc aatgccggca ccggtgaaga cggaattaaa
    30001 cgcgcgcgcc gggtctgtgc cgagctggcc aaagcgcggg attgcgagac ggagcaaatc
    30061 ctgccgtttt ccaccggcgt catcatggag cctttaccgg tggagcgcat tgtggccggc
    30121 ctgccgcaat gccttgccgg tttgcgcgca gacaattggc tggcggcggc acaggccatc
    30181 atgaccaccg acaccgtgcc caaggcgaga tcaaaacaaa tcagcatcaa cggcgcgacg
    30241 gtgacgatca ccggcatcgc caaaggggcg ggcatgatac gccccaactt ggccaccatg
    30301 ctcgctttca tcgccagcga cgcaagagta agccaggcgg cgctgcaagc cgtggtgagc
    30361 catgctgcgg cgcattcgtt taattgcatt acagtggatg gcgatacttc caccaacgat
    30421 gcgtttatgc tgatcgcttc gggccaggcg ccgatgccgg aaattgcggc tgcgacgagc
    30481 cgcgaattca ccgcctatcg cgatgcggta accggggtgg cgcagtggct ggcgcagtcc
    30541 attgtgcgcg acggcgaggg cgccaccaag ttcatcaccg tgcgggtgga gcagggaaaa
    30601 gacgcggcgg aatgcgccag ggtggcgtat gccatcgcgc attcgccgct ggtgaagacc
    30661 gcttttttcg ccgcggatcc aaacctgggc cgcatcctcg cggcgatcgg ttatgccggc
    30721 atcgccgacc tggatgtgaa caaggtgagc ttgtatctgg atgaagtttt ggtggcggaa
    30781 aacggcggac gcgcggcgag ctacaatgaa gcagcgggac ggaaagtgat gagcaggccg
    30841 gagatcgccc tgcgggtaag gcttggccgc ggacaggcgg attacacggt gtggacctgc
    30901 gatctgtctc acgattatgt gaccatcaac gctgaatatc gtacataggt gggcgcgata
    30961 atgaaaaacc ggtctcggca gatcccgttg aacgaactgc acgacatgct gctgcggatc
    31021 ggagatttac tgggcaggct ggagctggtg cttccggcgg cgccacggcc gccggactgg
    31081 caggcttcga tcgcgttccg ctggcgccag cgctacggca ggggattcct gcagccggtt
    31141 cctcacccgc accgcatccg gctctcggat ttactcggca tcgaacagca aaaacaactc
    31201 atcgatcaaa atacccggca gttcattcaa ggcttccccg ccaacaacgt cctgctcacc
    31261 ggcgcgcgtg gcacggggaa atcctcgttg gtgaaagcgg tactcaacaa atatgcgggc
    31321 aagggcctgc gcttgatcga ggtcgataaa cacgacctca tcgatttgcc tgacatcgtc
    31381 gagccggtgt gccggcgccc cgagcgcttg atcctgtttt gcgacgatct gtcgttcgaa
    31441 gcggacgagc ccggctacaa ggcgctgaaa gcggcgctgg aaggatcgat caccgcggct
    31501 tcggagaaca tgttggttta cgccacgtcc aaccgccgcc acctgatgcc ggaatacatg
    31561 gaggaaaacc tggcgacccg ccatgtcggg gacgaagtgc atccggcgga agcggtggag
    31621 gagaaaatct ccttttccga gcgcttcggc ctgtggcttt cgttctatcc cttcagccag
    31681 gatgattatc tcggcatcgt ggaccattgg cttggttttc tcggtgtgcg tcacgccgat
    31741 accgatgaag tgcggcaggc cgccgtgcag tgggcgcttg cgcggggttc ccgctcaggc
    31801 cgcgcggcgt ggcaattcgc ccgcgactgg gcgggacggc acgccctcaa aggcaaatga
    31861 agtcagccgt aggaagcagc aatgcaccgg cggatcctcg ccagctggtc aaagttgctg
    31921 cggcagtgat tctgcgtccg gacggccaat tcctgctggc gcagcgcccg gcaggcaaac
    31981 cttatcccgg ttactgggaa tttcccggcg gcaagatgga agcgcaggaa agcagcaagc
    32041 aggcgctcga gcgcgagcta aaggaagagc tgggtatcca catcgaggaa atttatccat
    32101 ggatcacgtg catctaccat tacacgcacg ccagcgtgga gctgcatttt catcgggtcg
    32161 tgcgctggac gggacaactc cacggcaggg aaagccagca gctcgcctgg caaagcgtga
    32221 aggccgtcga ggtcgcgccg ctgttgccgg cgaacagcgc agtgctccgg gcgctgactc
    32281 ttcccgaaca ttatgccatt agcaatgtgg cggaattggg ccgggagaaa ttcatgcgcc
    32341 gcctcgagcg atccttgcaa ggaggcttgc gcctggtcca gctgagag
//
LOCUS       DATIOW010000227         3818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_199236, whole genome shotgun sequence.
ACCESSION   DATIOW010000227 DATIOW010000000
VERSION     DATIOW010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199236"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VLL03_07635"
     CDS             complement(<1..398)
                     /locus_tag="VLL03_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013522734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSS47267.1"
                     /translation="MVLAKRTFDAVIVGGGGSGLRAALQLSDAGLKCAVLSKVFPTRS
                     HTVAAQGGIGASLGNMGEDNWLWHMYDTVKGSDYLGDQDAIEFMCRMAPQVVYELEHF
                     GMPFDRNEDGTIYQRPFGGHSRNFGERPVQ"
     gene            complement(399..746)
                     /gene="sdhD"
                     /locus_tag="VLL03_07640"
     CDS             complement(399..746)
                     /gene="sdhD"
                     /locus_tag="VLL03_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HSS47268.1"
                     /translation="MVKRIVLGAHYGLRDWLVQRVSAVVMVVYTLILLCVLLIQPSLQ
                     YAEWKALFSDQWMRLTSLLFLLSMFIHAWIGVHDVLMDYIQPTGVRLLLQAPVILALI
                     GYAAWSIQILWGV"
     gene            complement(740..1114)
                     /gene="sdhC"
                     /locus_tag="VLL03_07645"
     CDS             complement(740..1114)
                     /gene="sdhC"
                     /locus_tag="VLL03_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HSS47269.1"
                     /translation="MSEKRPKHLDLLKIRLPLPGWVSILHRISGVALFLFIPFLLWLL
                     QLSLVSPESFAGFKSLLANPLVKLILFGLLWAFLHHFLAGIRYLTLDVHWGTELSQAR
                     ATSMAVLVVSIVLTLLIGTRLW"
     gene            complement(1140..1874)
                     /locus_tag="VLL03_07650"
     CDS             complement(1140..1874)
                     /locus_tag="VLL03_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HSS47270.1"
                     /translation="MNHANPSPAFRPLYQQIKILITQSLISGEWRPGEAIPSETELAS
                     RYNVSQGTVRKAISELADENLLIRHQGKGTFVASHTEERRQFHFVRITSDKGGVIYPE
                     GELLECRRGKADSATAKLLEIHSGASLVVIRRLLKESAKPVLLEEIRIPAALFKGLND
                     EVINQHRCKIYSMFESAFGIRITQVVEQLKAVAASSEAAKLLKLAEGAPLLSIERVAY
                     TYGDKPVEWRKSLCNAKEHYYMNKIV"
     gene            2094..3080
                     /locus_tag="VLL03_07655"
     CDS             2094..3080
                     /locus_tag="VLL03_07655"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006028603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HSS47271.1"
                     /translation="MKKPVRVAVTGAAGQIGYSLLFRIANGDMLGKNQPVILQLLEIP
                     DEKVQAALKGVMMELEDCAFPLLAGMTAASDPKAAFRDADTALLVGARPRSKGMERKD
                     LLEANGAIFTVQGRALNEVASRNVKVLVVGNPANTNAYIAMNSAPSLPRASFTAMLRL
                     DHNRALSRLAVKTGKPVDSIEKLVVWGNHSPTMYPDYRFATIDGRSVKALINDDKWNR
                     EIFLPTVGKRGAVIIEARGLSSAASAANAAIDHMRDWTQGTNGKWVTMGIASDGSYGI
                     PEGIIYGFPVTCSGGKYQIIKGLEIDGFSREKMDATLKELQEERDGVKHLLV"
     gene            3112..>3818
                     /gene="prpB"
                     /locus_tag="VLL03_07660"
     CDS             3112..>3818
                     /gene="prpB"
                     /locus_tag="VLL03_07660"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HSS47272.1"
                     /translation="MSPVSPGARFRTAVAQEKPLQVAGCINAYCARMAERVGFKAIYL
                     SGGGVAAGSLGLPDLGIGTLDDVLTDVRRITDVSSLSLLVDVDTGFGSAFNIARTVRS
                     LIKFGAAAMHIEDQVQAKRCGHRPGKAIVSKEEMVDRIKAAVDAKTDADFVVMARTDA
                     LAVEGLQAAIDRACACAEAGADMIFPEAMTALDMYKQFADAVKVPVLANITEFGATPL
                     FTLDELRSVNVSLALYPL"
BASE COUNT          885 a         1102 c         1024 g          807 t
ORIGIN      
        1 ctttgcaccg gccgctcgcc gaaattccgc gagtgcccgc caaacgggcg ctgataaata
       61 gtgccgtcct cgttgcggtc aaacggcatg ccgaagtgct caagttcata caccacctgt
      121 ggcgccatgc ggcacataaa ctcaatcgcg tcctggtcgc cgaggtaatc ggaacccttc
      181 accgtgtcat acatgtgcca cagccaattg tcttcgccca tgttgccgag cgacgccccg
      241 atgccgcctt gcgccgcgac agtgtgagag cgcgtgggaa acactttcga cagcaccgcg
      301 catttcaacc cggcatcgga aagctgcaac gcggcgcgca agccggagcc gccgccgccg
      361 acgatcaccg catcaaatgt tcttttcgcc aacaccattt acacgcccca aagaatctgg
      421 attgaccacg ctgcatagcc gatcagtgca agaattaccg gcgcctggag caacaagcgc
      481 acgccggttg gctggatgta atccatgaga acgtcgtgta cgccgatcca tgcgtggata
      541 aacatgctca acaaaaaaag caggcttgtc agacgcatcc actggtcact gaacagcgct
      601 ttccattcgg catactgcag cgacggctgg atcagcagca cacaaagcag aatcaacgta
      661 tagacgacca tcaccaccgc gctcacgcgc tgcaccagcc agtcgcgcag gccgtaatgg
      721 gcgccgagga cgatccgctt caccatagcc tcgttcctat tagcaacgtt agcacgatgc
      781 tcacaaccaa taccgccata ctggtcgccc gcgcctggga aagctcggtg ccccaatgta
      841 catccagcgt cagatagcga atgccggcaa ggaagtggtg cagaaatgcc cacagcaagc
      901 cgaacaggat taatttcacc agcggattgg ccaacaagga cttgaagccg gcaaaacttt
      961 ccggtgaaac aagactcaat tgcagcagcc agagtaaaaa aggaatgaac agaaacagag
     1021 ccacgccgct gatgcggtgc aggatggaaa cccagcccgg aagcggcagc cgtattttca
     1081 gcaaatcaag atgtttaggc cgtttttcgc tcatttgttt ttaattatat tacattgact
     1141 tacactattt tattcatgta atagtgttct ttggcattgc acaggctttt gcgccactct
     1201 accggtttat ccccataggt gtaagctacg cgctcgatgg aaagcagcgg cgcgccctcg
     1261 gccagcttta ataatttggc ggcctcgctg ctggcagcca ctgccttgag ctgttcgacc
     1321 acctgggtaa tacgaatgcc gaaggcggac tcgaacatac tgtagatctt gcaccggtgc
     1381 tggttgatga cctcgtcatt aagacccttg aacagcgcgg caggtatgcg gatttcttcc
     1441 agcagcaccg gttttgctga ttccttgagc aaacggcgga tcactaccag cgaagcgccg
     1501 ctgtggattt ccaggagttt ggccgtggcg ctatcagcct tgccacgccg gcattccaga
     1561 agctcccctt ccggataaat caccccgcct ttgtccgagg taatgcgcac gaaatgaaac
     1621 tgacgccgct cctcggtgtg tgaggcaaca aaagtgcctt tgccctgatg gcggatcaga
     1681 agattttcat cggcgagttc ggaaatcgcc ttgcgcacgg tgccctggct tacgttgtag
     1741 cgtgaagcca gctcggtttc gctgggaatc gcttctcccg gacgccactc cccggaaatc
     1801 agactttggg tgatcaagat cttgatctgc tggtagagag ggcgaaaagc cggggatgga
     1861 ttggcgtggt tcattggcct caatttctaa cataaaattg gtgcctcgtc catgctatta
     1921 tgtatctttt ataagatata agaattgatt gacaggggct ggaattattt cataccatgc
     1981 ctgaaattta tgcggctttt ttctagcccg gctcaccgcg gaaaacatcc tgaatttatc
     2041 ctcatattaa acttgaagaa aagttcttaa tgatataatt tataaaactt ataatgaaaa
     2101 aacccgttcg tgttgcggtt accggtgccg ccggccaaat cggctatagc cttcttttca
     2161 gaatcgctaa cggcgatatg ttgggcaaga accagccggt tatcctacag ttgctcgaga
     2221 tccccgatga aaaagtccag gcggcgctca aaggggtgat gatggaactc gaggactgcg
     2281 cctttcccct gcttgccggt atgaccgccg cttccgatcc aaaagccgca ttcagggatg
     2341 ccgatacggc gcttctggtc ggcgcccgtc cccgcagcaa aggcatggaa agaaaagatt
     2401 tgctcgaggc gaacggcgcg attttcaccg tgcagggacg cgcgctcaat gaggtggcaa
     2461 gccgcaacgt gaaagtgctg gtggtgggaa accccgccaa caccaatgcc tacatcgcta
     2521 tgaacagcgc gcccagtctt ccacgcgcat ccttcaccgc catgctgcgg ctcgaccata
     2581 atcgcgcgct gtcgcgactc gccgtcaaaa ccggcaagcc ggtggattcg atagaaaaac
     2641 tggtcgtctg gggcaatcat tcgcccacca tgtatccgga ttaccgcttc gccaccattg
     2701 atggccggag cgtgaaagcc ttgatcaacg acgataaatg gaaccgcgag atcttcctcc
     2761 ccaccgtggg taaacgcggg gccgttatca tcgaagcgcg cggcctttca tccgcggctt
     2821 ccgccgccaa tgccgcaatt gaccacatgc gcgactggac gcagggcacc aatggcaaat
     2881 gggtcaccat gggtattgcc tccgacggca gttacggtat tcccgaaggc attatttatg
     2941 gcttcccggt aacctgttcc ggcggcaaat atcaaatcat caaagggttg gaaatcgacg
     3001 gcttctcacg ggaaaaaatg gacgcgacgc ttaaagaact gcaggaagag cgcgacggcg
     3061 tgaagcacct gctcgtttga tcagacgcac atttttaacc aaggaactcc catgagccct
     3121 gtctcgccag gcgcacgctt ccgcaccgcc gtcgctcagg aaaaaccgct gcaggtggcg
     3181 ggttgcatca atgcttattg cgcgcgcatg gcggaacgag tcggcttcaa ggcaatttat
     3241 ctttccggcg gcggcgtcgc ggcgggctcg ctcgggcttc ccgatctggg catcggcaca
     3301 ctcgatgatg tgctcaccga tgtgcggcgc attaccgatg tcagcagctt gtcgctcctg
     3361 gttgacgtgg acaccggttt cggcagcgct ttcaacatcg cgcgaacggt aaggtcgctg
     3421 atcaaattcg gcgcggcggc gatgcacatt gaagatcagg tccaagccaa gcgctgcggc
     3481 catcgtcccg gcaaagccat cgtctccaag gaagaaatgg tggaccgcat caaagccgcg
     3541 gtggacgcca aaaccgatgc cgatttcgtg gtgatggcgc gcaccgatgc gctggcggtg
     3601 gaaggcctgc aggcggcaat cgaccgtgcc tgcgcctgcg ccgaagctgg cgcggacatg
     3661 attttccccg aggcaatgac cgcgctcgac atgtataaac agtttgccga cgcagtcaag
     3721 gtgccggtgc tcgccaacat caccgagttc ggtgctacac ccttgtttac gcttgacgag
     3781 ttgcgttccg tcaatgtcag tctcgcgctc taccctct
//
LOCUS       DATIOW010000228         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1114803, whole genome shotgun sequence.
ACCESSION   DATIOW010000228 DATIOW010000000
VERSION     DATIOW010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114803"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..169
                     /locus_tag="VLL03_07665"
     CDS             <1..169
                     /locus_tag="VLL03_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSS47273.1"
                     /translation="HFVRPALIGGAARLPFLWVLLGILGGLENLGLLGLFLGPAIMAA
                     LISLWRDRTAA"
     gene            186..455
                     /locus_tag="VLL03_07670"
     CDS             186..455
                     /locus_tag="VLL03_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HSS47274.1"
                     /translation="MVKRLRIRGLVQGVFFRETMRRQAEKFGVTGWVRNCSDMSVEAM
                     VQGTPEALDAIIAWAHQGPETAQVERVEIDQGEGNFTRFEVVRSR"
     gene            complement(457..>2153)
                     /gene="dnaE"
                     /locus_tag="VLL03_07675"
     CDS             complement(457..>2153)
                     /gene="dnaE"
                     /locus_tag="VLL03_07675"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HSS47275.1"
                     /translation="NDENISPFSLENIPLDDKPTYEIFASGNTTAIFQCESRGMREMM
                     IKARPDRFEDIIALIALYRPGPMELIPEFIRRKQGARVDYIDRRLEPVLSPTYGIMVY
                     QEQVMQIAQVIGGYNLGAADLLRRAMGKKDAKEMARHRAIFVMGAGKNGVPERRANDL
                     FDLMEKFAGYGFNKSHAAAYAVLAYQTAYLKAHFPAAFMAATLSADMDDTDKVQLLYK
                     DCLDNRLEILPPDINQSGYRFAPVGERQIRYGLGAVKGTGEAAIAAMVKARETGGSFR
                     DLFDFCLRVDKRIVNRRSIESLIRAGAFDSLNPHRASLLASVGIAMENAEQAQRSANQ
                     VSLFNELEGTAMHKPALIEVPKWPETLRLQNEKMALGFYLSGHPFTAYQKSVQGFVKT
                     RLSELTPQQQPVLLAGVIYSVRIQQTRRGRMAIVVLDDGSNQVETVVFSELFEANRVL
                     LKEDQLLIVEGKVGYDEYSGGLRITADKLYDLTAARNQFARVMRIRLNGEASAARLRT
                     LLGPYRKGLCPVAVRYYNAQAVCEVQLGEEWRVNLDDDLLRDLCEWLEPENVEVVY"
BASE COUNT          406 a          643 c          629 g          475 t
ORIGIN      
        1 tcatttcgtg cggcccgcgc tgattggcgg cgccgcgcgc ttgccttttt tgtgggtatt
       61 gctgggcatt ttgggtggcc tggaaaatct tgggttgctg ggtttgttcc tcggcccggc
      121 gatcatggcc gcgctgattt cgctctggcg cgaccgtacg gcagcctaag cggcggctca
      181 agccgatggt caagcgcttg cgcatacgcg gcctggtgca aggcgtgttt ttccgggaaa
      241 ccatgcgccg ccaggcagag aagttcggcg tcaccggctg ggtgcgcaac tgctccgata
      301 tgagtgttga agcgatggtg cagggaaccc ccgaagcgtt ggacgccatc atcgcctggg
      361 cgcatcaggg acctgaaacg gcacaggtcg agcgtgtgga aattgaccaa ggggaaggaa
      421 attttacgcg ctttgaggta gtgcgcagcc gctgatttag tagaccacct ccacgttctc
      481 aggctccaac cattcgcaca aatcacgcag caaatcgtca tcgagattga cccgccattc
      541 ctcgcccaac tgcacttcac acaccgcttg cgcgttgtaa taacggaccg cgaccgggca
      601 cagtccttta cggtaggggc cgagcaatgt ccgcagcctg gcggcactcg cttcgccgtt
      661 gagcctgatg cgcattaccc gggcgaattg gttgcgcgcc gcagtcaaat catacagttt
      721 gtcggcagtg attcttaagc ctcctgaata ttcgtcgtag ccaaccttgc cctcgacaat
      781 gagcaactgg tcctctttca ggagcacgcg gttcgcttcg aacaattcgc tgaacaccac
      841 tgtttcaact tggttgctgc catcatccag cactacgatc gccatcctgc cgcgccgcgt
      901 ctgctggata cgtacgctgt aaatgacgcc ggcgagcaaa accggctgct gctgcggcgt
      961 aagttcgctt aagcgcgttt tcacgaagcc ttgcacgctc ttctgatagg cggtgaacgg
     1021 gtggccgctc agataaaaac ccagcgccat tttttcattt tgcaatcgca gcgtttccgg
     1081 ccatttcggc acctctatca gcgcgggctt gtgcatggcc gtgccttcga gctcattgaa
     1141 caggctcacc tggttagccg agcgttgcgc ttgctcggca ttttccattg caatgccgac
     1201 cgaagcaagc aggctcgcgc gatgtgggtt cagcgaatca aaagcgccgg cgcggatcag
     1261 cgattcaatg gaacggcggt tgacgatgcg cttgtccacg cgcagacaga aatcgaagag
     1321 atcgcgaaac gacccgccgg tctcgcgcgc tttcaccatc gccgcaatcg ccgcttcacc
     1381 cgtgcctttc accgcgccca gcccgtagcg tatctgtctt tcacccaccg gcgcgaaacg
     1441 gtagccgctc tggttgatgt cgggcggcag aatttcgaga cggttgtcga ggcagtcttt
     1501 atagagaagc tgcaccttgt cggtgtcatc catgtcggcc gaaagcgtgg cggccatgaa
     1561 cgccgccggg aaatgcgcct tgagatacgc ggtctggtag gcgagcaccg cataagccgc
     1621 ggcgtgcgat ttgttgaaac cgtagcccgc gaacttctcc atcaggtcga aaaggtcgtt
     1681 ggctctccgt tccggcacgc cgttttttcc agcgcccatg acgaaaatgg cgcgatgccg
     1741 cgccatttcc ttcgcgtctt tcttgcccat cgcgcggcgc agcaggtcgg cggcgcccaa
     1801 gttgtagccg ccgatcacct gcgcgatctg catcacctgt tcctgataca ccatgatgcc
     1861 gtaggtggga ctcaaaaccg gctcgagccg ccggtcgatg taatccacgc gcgcgccttg
     1921 cttgcggcgt ataaactccg ggatgagttc catcggcccc ggacggtaga gcgcaatcag
     1981 cgcgatgatg tcctcaaaac ggtccggccg cgccttaatc atcatctcgc gcatgccgcg
     2041 cgattcacac tggaaaatcg cagtggtgtt gccgctggcg aatatttcgt aagtgggttt
     2101 gtcgtcgagg gggatgttct ccagagagaa cggcgagatg ttttcgtcgt tgc
//
LOCUS       DATIOW010000229         5059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_708258, whole genome shotgun sequence.
ACCESSION   DATIOW010000229 DATIOW010000000
VERSION     DATIOW010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708258"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VLL03_07680"
     CDS             complement(<1..685)
                     /locus_tag="VLL03_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSS47276.1"
                     /translation="MEQNLVAMLQFLGVRDFVIVDSMELEFKSGFTVLTGETGAGKSI
                     LIDALALTLGERADGAVVRQGSTRAEITAEFDITKQSKLSAWLRENDLEGDAGECLMR
                     RIIETNGKSRGFINGRAATVQQLREAGEKLVEIHGQHAHQTLLQADSQRELVDAFGGL
                     SELAAQTAGYYRKWQGLRKKRLEWETNAAAFAAEREQLEWQVRELKTLNFSADEWQTI
                     TMEHSRLSHA"
     gene            complement(667..1575)
                     /locus_tag="VLL03_07685"
     CDS             complement(667..1575)
                     /locus_tag="VLL03_07685"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002306.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HSS47277.1"
                     /translation="MADGYKICFNARMNNSFKIVALIGKYKSPEIVGHLLRLARFLEG
                     RGVTVLVDRLTSAHLKKNNYPVLTLEEIGKQADLAIVLGGDGTMLNIARTFAPYDVAL
                     VGVNQGRLGFLTDISVDTMLETIGAILDGKYVTEERMLLSAEIMSNSKSVLKVLTFND
                     VVVSKGAKGSLIEFDVHIDGQFVYNLRSDGLIVATPTGSTAYALSSGGPIVHPSLNLI
                     ALVPVCPHTLSNRPIVVGGNSIVEILMHDSGDARAHFDSHSHFDLRDGDRIILSRSND
                     TIRLLHPVGHDYYSMLREKLHWSKIS"
     gene            1697..2716
                     /gene="hrcA"
                     /locus_tag="VLL03_07690"
     CDS             1697..2716
                     /gene="hrcA"
                     /locus_tag="VLL03_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HSS47278.1"
                     /translation="MLSERAQILLKTLVERYISDGQPVGSRALSQYSGLDLSPASIRN
                     IMAGLEEMGFVASPHTSSGRVPTPLGYRFFVDTLLTVRPLDQVQIDQLETQLQTENTQ
                     KLMTSASHLLSDLTHFAGVVMNPKRHSATFRHIEFLSLAEKRILLIIVTPEGDVQNRI
                     LFTDKTYTPSELTQAANFLNQHYAGKTFDEIRRAVQDELRQLHSDMSLLMTAALEVGS
                     QAVWESASDYVISGEHNLLHVQDLSSNMARLRRLFDLFEQKTALLQLLDSSGRAHGVQ
                     IFIGGESGLVPLDECSVVTAPYEVDGQVVGTVGVIGPTRMAYERVIPIVDITAKLLTS
                     ALSQH"
     gene            2780..3871
                     /gene="hemH"
                     /locus_tag="VLL03_07695"
     CDS             2780..3871
                     /gene="hemH"
                     /locus_tag="VLL03_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HSS47279.1"
                     /translation="MKPEPPYQHGTPAQTGILLINLGTPDAPTARALRPYLKQFLSDR
                     RVVEIPRLIWWPILHGIILNTRPKKSAAKYAKIWMAEGSPLRVHTERQTKFLRGYLGE
                     RIKSPLTIEYAMRYGKPSIASALARLKHCERILVLPLYPQYSAAATGSSFAAVFREFS
                     RMRNTPAVRTVKHYHDHPSYIAALAQSVRDYWMQNGRPDQLLISFHGVPRFTLDKGDP
                     YHCECQKTGRLLAEALGLDEDRYTVCFQSRFGRAEWLEPYTSEVLGKLGKKRIERVDV
                     ICPGFVSDCLETLEEIAIEGKATFLGSGGKEFHFIPCLNERSDWMHALSEIALEHLHG
                     WVSVQYNAEAAAQAGRLARERALGLGAKA"
     gene            3954..4514
                     /gene="grpE"
                     /locus_tag="VLL03_07700"
     CDS             3954..4514
                     /gene="grpE"
                     /locus_tag="VLL03_07700"
                     /inference="COORDINATES: protein motif:HMM:NF010737.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HSS47280.1"
                     /translation="MMQNSQENQPSTEPTPEPGVAAQEKAEQKTETMPSLEELLKKAE
                     LTAEEHHDAWLRAKAETENIRKRAQIEIASAHKYAIENFSTALLAVKDSLEATLAAEN
                     ATSESLKSGVELTLKLLGTAFERFKISEINPRGEKFDPHKHQAMTTVESDAPPNTVIS
                     VMQKGYMLHDRVIRPALVVVGKARET"
     gene            4578..>5059
                     /locus_tag="VLL03_07705"
     CDS             4578..>5059
                     /locus_tag="VLL03_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HSS47281.1"
                     /translation="MAKIIGIDLGTTNSCVAIMESGKPKVIENSEGARTTPSIVAYSD
                     EDEVLVGASAKRQAVTNPKNTLFAVKRLIGRRFSEGEVKKALKLMPYKIIENSNGDAW
                     VEVRGKKIAPPEVSAQVLMKMKKTAEDYLGEKVEEAVITVPAYFNDSQRQATKDAGRI
                     A"
BASE COUNT         1158 a         1509 c         1411 g          981 t
ORIGIN      
        1 cggcgtggga caggcggctg tgttccatgg taatggtctg ccattcgtct gcggaaaaat
       61 tcagggtctt gagttcgcgc acctgccatt ccaattgctc gcgctcggcg gcaaaggccg
      121 cggcgttggt ttcccattcc agccgttttt tgcgcaaacc ctgccatttg cgatagtacc
      181 cggcagtctg cgccgcgagt tcgcttaaac cgccaaaggc gtcgaccagc tcgcgctgcg
      241 aatcagcttg aagcagcgtc tggtgcgcat gctggccgtg gatttcaacc agtttttctc
      301 ccgcttcacg caattgctgc acggtggcgg cgcggccgtt gatgaagccg cgcgatttgc
      361 cgttggtttc gattatccgg cgcatcaagc actcgccggc atcgccctcc agatcatttt
      421 cacgcagcca agcggaaagc ttagattgtt ttgtgatatc gaattcggcg gtgatttcgg
      481 cgcgcgtcga gccttgccgc accaccgcgc cgtcggcgcg ctcgcccagg gtgagcgcca
      541 gcgcatcaat cagtatggat tttccggcgc cggtttcgcc cgtcagcacg gtgaagcccg
      601 acttgaattc gagctccatc gaatcgacga tgacgaaatc gcggacacct agaaattgca
      661 gcatggctac gagattttgc tccaatgcag cttttcgcgc agcatgctgt agtagtcgtg
      721 ccccaccgga tgcagcaggc gaatggtgtc attggaacgg ctcaagatga ttctgtcgcc
      781 gtcgcgcaaa tcgaaatgcg aatggctgtc gaagtgcgcc cgcgcatcgc cggaatcgtg
      841 catgaggatt tcgacgatgg aattgccgcc caccacgatg ggccgattgc tcaaggtgtg
      901 tggacaaacc ggcaccagag caatcaggtt caacgacgga tgcacaatcg gtccgccgga
      961 agacaacgca taagccgtgg agccggtggg cgttgccact atcagtccgt cagagcgcag
     1021 gttgtagaca aactggccgt cgatatgcac gtcgaactcg atgaggctcc ccttggcgcc
     1081 cttgctcacc accacgtcgt tgaacgtcag cactttgagc acgctcttgc tgttgctcat
     1141 gatttcggct gacagcagca tgcgctcctc ggtcacatac ttgccgtcga ggatggcgcc
     1201 gatggtttcc agcatggtgt ccactgaaat gtcggtgaga aaccccagcc gcccctggtt
     1261 gacgccgacc agcgccacat cgtagggagc aaaggtgcgc gcgatgttga gcatggtgcc
     1321 gtcgccgccc agcacgatgg cgaggtccgc ctgcttgcct atttcctcga gtgtcaaaac
     1381 cggatagttg tttttcttga gatgtgccga ggtgagccgg tccaccagca cggtcacgcc
     1441 gcggccttcc agaaagcgcg caaggcgcag caggtggccg acgatttccg ggcttttgta
     1501 tttgccgata agggcgacga ttttaaacga attattcatc cgggcattaa agcatatttt
     1561 atagccatca gccatcagcg gtcagcagag cccgcggcgt agccgcgcca gattcaagct
     1621 gaaagctgat cgctgatggc tgaatcctaa ttatgaccgc tgattgccga cagctgaaag
     1681 ctgctacaat aaatcgatgc tcagcgaacg cgcgcaaatc ctgctgaaaa cactagtgga
     1741 acgctacatt agcgacggcc agccggtagg ctcgcgcgca ctttcccaat actccgggct
     1801 ggatttgagc ccggccagca tccgcaacat catggccggt ctagaagaaa tgggctttgt
     1861 cgcaagcccg catacttcct ccgggcgcgt gcccacgccg ctcggctacc gcttcttcgt
     1921 ggacaccctg ctcaccgtcc ggccgctgga tcaggtgcag attgaccagc tggaaaccca
     1981 gctgcagacc gaaaacacac aaaagctcat gacctcggct tcacacctac tctccgacct
     2041 gacccacttc gcgggcgtgg tgatgaatcc gaagcgccat agcgcgacat tccgtcacat
     2101 cgagttcctg agtctcgccg aaaaacgcat cctgctcatc atcgtcacgc cggaaggtga
     2161 tgtgcaaaat cggattctct tcaccgacaa gacctacacg ccttcggagc tcacgcaagc
     2221 ggcgaatttc ctcaaccagc actacgccgg caagacgttt gatgaaatcc gcagggcggt
     2281 ccaggacgaa ctaagacagc tccacagcga catgagcctg ctgatgaccg cggcgctgga
     2341 agtcggcagc caggccgtgt gggaatcagc cagcgattac gtgatttccg gcgagcacaa
     2401 tttgttgcac gtgcaggatc tttcttccaa catggcaagg ctgcgcaggc tctttgattt
     2461 gttcgagcag aaaaccgcgc tgctgcaact gctcgacagc agcggccgcg ctcacggggt
     2521 gcagattttc ataggcggcg aatcgggact ggtgccgctc gacgagtgca gcgtggtcac
     2581 ggcgccgtac gaagtggatg gccaggtggt cggcacggtc ggcgtcatcg gcccgacgcg
     2641 catggcctac gagcgggtga ttcccatcgt cgacatcacc gccaaactcc ttaccagcgc
     2701 gctttcacag cattgataca gtgaatagtg gctagtggat agtggatagt aaaattaaaa
     2761 accaaccacg gccctcacca tgaaacccga gcccccgtac caacatggca cgccggcgca
     2821 aaccggcatt ctgttgatta acctcggcac gccggatgcg cccaccgcca gggcgctgcg
     2881 tccttatctc aagcaatttc tctccgaccg gcgggtggtg gaaatcccgc gcctcatatg
     2941 gtggccgatt ctgcacggca tcatcctcaa cacacggccg aaaaaatccg ccgccaaata
     3001 cgccaaaatc tggatggccg aaggctcgcc gctcagagtt cacaccgaac gtcagacgaa
     3061 attcctgcgc ggctatctgg gcgagcgcat caaatctcct ctcaccatcg aatacgcgat
     3121 gcgctacggc aaaccttcga ttgcttccgc tctcgcccgg ctcaagcatt gcgagcgcat
     3181 cctggtgctg ccgctttatc ctcaatattc cgccgccgcc accggcagca gttttgccgc
     3241 ggtattcagg gaatttagcc gcatgcgtaa cacaccggcg gtgcgcacgg tgaagcacta
     3301 tcacgaccac ccgagctaca ttgcggctct cgcccaaagc gtacgcgact actggatgca
     3361 gaacggccgt cccgaccagc tgctcatcag cttccacggc gtgccgcgct tcaccctgga
     3421 taaaggcgac ccttaccact gcgaatgcca gaaaaccgga agacttttag cggaggcgct
     3481 gggattggac gaggatcgtt ataccgtgtg cttccagtcg cgctttgggc gcgcggaatg
     3541 gctggagcct tatacgtccg aagtgctggg aaagctcggc aaaaaacgca tcgagcgcgt
     3601 ggacgtgatt tgccccggct ttgtcagcga ttgcctggaa acactggagg aaattgccat
     3661 cgaaggcaaa gcaacattcc tgggcagcgg cggcaaggaa ttccatttca tcccctgcct
     3721 caacgagcgc agcgattgga tgcacgcact ctctgaaatc gcgctcgagc acctgcacgg
     3781 ctgggtttcg gtgcaataca acgccgaagc cgctgcacag gcgggacggc tggcgcgcga
     3841 gcgcgcgctg ggcctgggcg ccaaagcctg agcctgttcc gatctttaat ttccagtttc
     3901 cccttgaaat cactcgaatt gcccccaaat ttattttcgt ttaacaggag agaatgatgc
     3961 aaaattctca agaaaatcag ccgtctaccg aacccacgcc tgaaccaggg gttgcggctc
     4021 aagaaaaagc ggagcaaaaa accgaaacca tgcccagcct ggaagagctt ttgaaaaaag
     4081 ccgaactcac agcggaagaa catcacgatg cgtggctgcg ggcaaaagcg gagaccgaga
     4141 atatccgcaa gcgtgcgcaa attgaaatcg ccagtgcgca caaatatgcg attgaaaatt
     4201 tctcgacggc gttgctcgcg gtgaaagaca gcctggaagc cactctggcc gcggaaaacg
     4261 ccacttcgga gagtctgaaa agcggcgtgg agctcacgct gaagctcctc ggcacggcgt
     4321 ttgaaagatt caaaatcagc gaaattaacc cgcgaggcga gaagtttgac ccgcataagc
     4381 accaagccat gaccacggtg gaatcggacg cgccgcccaa caccgtgatc agcgtcatgc
     4441 aaaaaggcta catgctgcac gaccgcgtga tccgccccgc gctggtagtg gtgggtaagg
     4501 ccagagaaac ttgaatttca cgtatttcac cctgatatag caagcaactg tcaaccattc
     4561 atcgttaagg aattatcatg gcaaagatca tcggaatcga cttggggacc accaactcct
     4621 gcgtcgccat catggagagc ggcaagccca aggtgattga aaactccgaa ggcgcccgca
     4681 ccaccccttc cattgtcgcc tactccgacg aagacgaagt tctggtcggc gcctcggcca
     4741 agcggcaggc ggtgaccaac ccgaagaaca ccctgttcgc ggttaagcgg ctcattgggc
     4801 ggcgcttcag cgaaggcgaa gtgaagaaag ccctcaagct catgccctac aaaatcatcg
     4861 agaacagcaa cggcgacgcc tgggtggagg tacgcggcaa gaaaatcgcg cctcccgaag
     4921 tgtcggcgca ggttttaatg aaaatgaaga aaaccgccga ggattacctg ggcgagaaag
     4981 tggaagaagc ggtcattacc gttcccgcct acttcaacga ctcgcagcgg caggcaacca
     5041 aggacgccgg gcgcattgc
//
LOCUS       DATIOW010000230         3266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1723546, whole genome shotgun sequence.
ACCESSION   DATIOW010000230 DATIOW010000000
VERSION     DATIOW010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1723546"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1500)
                     /locus_tag="VLL03_07710"
     CDS             complement(<1..1500)
                     /locus_tag="VLL03_07710"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HSS47282.1"
                     /translation="MSLLAGFLSVLFHSLVLIGMSVAVGGLIFLLVVLLPLAKSNSDA
                     HNAIRRSLLILVWGSALLAAAQTLGLLMEPWALADDFGAWPMAAFFSTGFAQAGLIHV
                     AFAVALTFTGVWLIKQPASRAAWSAAVTAAILLMASGAWLVHAVGRLESTVLLMLMTV
                     LHQLGAAVWVGGIIHLLGLWRLTRRSGSSLWPRALARFSPLAVLGVVLLIFCGIYLWW
                     SYIGNWEGLIGTAYGVMVLTKVALLFAALVLGAMNFFLTRRWLRLGDTQGVGKRVPSF
                     IEAEFNIVIIILLTAAALTSLPPSIDVAAEKATPSEVWDALKPGKPRLVPPPRQEYLA
                     SSTSSFDVFTLQNDFDKRQSEFNHNVSGLLVLIVGLAAVLDRTGKFPWARHWPLLFML
                     VGVFLVLVGEPNGWPLGYEGFWETLVIPSVIQHRLATVVVIGLALFEWRVRVGGLAET
                     RWRYAFPLLCMTGGALLLTHSHTLLTNKYQFLIEVSHALLGFFAVMIGVG"
     gene            complement(1504..1872)
                     /locus_tag="VLL03_07715"
     CDS             complement(1504..1872)
                     /locus_tag="VLL03_07715"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HSS47283.1"
                     /translation="MRKPKLFAATSALLFLTSYNLCWAHAIVIESNPREGAVLKRAPD
                     RIQLRFNVKIEKALTRVTLIKGDKQAISLPQADFNRGAPERLEVPLPRLEPGNYMLRY
                     SVLAADGHATQGVLRFSIEK"
     gene            complement(2021..3013)
                     /locus_tag="VLL03_07720"
     CDS             complement(2021..3013)
                     /locus_tag="VLL03_07720"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HSS47284.1"
                     /translation="MKLAIAIAALAGLLAAILLIGYHGFIAVSDLLLSVGWGMVVVSL
                     FHLLPVTLSSLAWRRVLRNTWSKSLLVFFWARSVREAVGSLLPVMQVGGELVGARILT
                     FNGAKAGIAGASVVVDLTLEAIAQFFFTLLGLLLLVLIGGSEQTVRWAAAGLVLAALA
                     LAGFVIAQHKGMFKLFENFIEKIADKWEWLSLGKIDNLHDTIQKLYRERRALLAAGVT
                     HLLSWIVSATEVWLALYFMGSPISFLEALILESLSHAVKSAAFVIPGALGVQEGAYML
                     LGEMLGLTPEMGLALSLVKRVRDLLLGIPALLGWQAQESGRLWIKANRRKSDGH"
     gene            complement(3010..>3266)
                     /locus_tag="VLL03_07725"
     CDS             complement(3010..>3266)
                     /locus_tag="VLL03_07725"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HSS47285.1"
                     /translation="IAFGIYLVMRKRGGLDVEHLFRSSTPRAVLFSALTTVASFGSLA
                     FSEHRGMSGLGLLLTIALSFALLCTLVVLPAIMSVAEEKK"
BASE COUNT          800 a          979 c          920 g          567 t
ORIGIN      
        1 cccgactcct atcattaccg cgaaaaagcc gagaagtgca tgcgaaacct ctatgaggaa
       61 ctggtatttg ttggtgagca gggtgtggga atgggtgagc aacaacgcgc cacccgtcat
      121 gcataacaaa gggaaggcat agcgccagcg cgtctcggcc agcccgccta cgcgcacccg
      181 ccactcaaat aacgcaagac caatcaccac cacggtggcc agccggtgtt gaatgactga
      241 gggtattacc agggtttccc aaaaaccttc gtaaccgagc ggccagccgt ttggctcgcc
      301 gaccagcacc agaaacaccc cgaccagcat gaataacagc ggccaatgcc gcgcccaagg
      361 aaatttaccg gtgcgatcca gcaccgccgc caacccaaca atgagaacca gcaggcctga
      421 aacattgtgg ttgaattcgc tttgtctctt gtcgaagtcg ttctggaggg taaatacgtc
      481 gaatgaagaa gtagagctcg ccagatattc ctggcgcggc ggcgggacga gacggggttt
      541 accgggcttc aacgcgtccc agacttcgct gggtgtggcc ttctccgccg cgacgtctat
      601 tgaaggcggc agcgaagtta gtgcggcggc ggtgagcaaa atgatgatga cgatgttgaa
      661 ttcggcttca atgaaagacg ggacgcgttt tccaacgccc tgcgtgtcgc ccagccggag
      721 ccagcgccgc gtgagaaaaa aattcatcgc acccagcaca agcgcggcga atagcaaggc
      781 cactttggtc agcaccatca cgccgtaagc ggtgccgatg agcccttccc agttgccgat
      841 ataactccac cacagataaa ttccgcaaaa gatgagcaag acaacgccga gcacagcgag
      901 cggcgagaag cgtgccagcg cacgcggcca caaggacgaa ccgctgcgac gcgtgagacg
      961 ccataacccg agaagatgga ttattcctcc cacccagacc gcggcaccga gctgatgcag
     1021 caccgtcatc aacataagca gcacggtgct ctcgagacga cccactgcat gcaccagcca
     1081 tgccccgctt gccatgagta gaatcgccgc cgtgacggct gcgctccacg cagcgcgtga
     1141 tgcaggttgt ttgataagcc atacgcccgt gaaagtcaga gcgacggcaa aggcgacatg
     1201 aatcaggccg gcctgggcga agccggtgga gaagaacgcc gccataggcc acgcaccgaa
     1261 atcatcagcg agtgcccacg gctccatcag cagtcccagc gtttgtgcag ctgccagcag
     1321 tgccgagccc cagacgagga tgaggaggga acgtcttatt gcattatgcg cgtcgctatt
     1381 gcttttggcg agcggcaaaa gcacgaccag gaggaaaatc agaccgccga ccgcgactga
     1441 catgccgatc agcaccaggg aatgaaacag gaccgagaga aaaccggcga gcaggctcat
     1501 ggactatttc tcgatactga agcgcagcac gccttgggtg gcgtggccgt ctgcggccag
     1561 caccgaatag cgcagcatat aatttccggg ctccagacgc ggcaacggca cctcaagccg
     1621 ctccggcgcg ccccggttga aatcggcctg cggcaaagag atcgcctgct tgtcgccttt
     1681 gatcagagtt acgcgggtga gcgctttttc gattttgacg ttgaagcgca gctggatgcg
     1741 gtcaggtgcc cgcttgagca ccgcgccttc acgcggattg gattcgatga cgatggcatg
     1801 cgcccagcaa agattgtaac tggtcaggaa cagcaacgcc gacgtagcgg caaacagttt
     1861 gggttttctc atggctgctg aaatcggtgt aaggcgcgta ccagcggcgc ggcaaaactg
     1921 acccgataag tgtctcacaa aaaagcgcgc atattttatc agcacaagat tcagttcaaa
     1981 aactttttgc tgtgcattca tctgcaaatg taatgctttc tcaatgcccg tcgctcttgc
     2041 gccggttcgc cttgatccac aggcgtccac tttcctgcgc ctgccatccc aagagcgcgg
     2101 gaattccgag cagcagatcg cggactcgtt tcaccaggga cagcgccaat cccatttcgg
     2161 gagtaagacc cagcatttca cccagcagca tgtaggcgcc ttcctggacg cccagcgcgc
     2221 cgggaatcac aaatgcggcg ctttttaccg cgtggctcaa gctttcgaga atcagcgctt
     2281 cgaggaaact aatggggctg cccatgaaat aaagcgcgag ccagacttcg gtggcgctga
     2341 caatccagga gagaagatgg gttacgcccg cggcaaggag cgcgcgccgc tcgcggtaga
     2401 gtttctgaat ggtgtcgtgc aggttgtcga tttttccgag cgacagccat tcccatttgt
     2461 ccgctatttt ttctataaaa ttttcgaaca acttgaacat gcctttgtgc tgggcgatga
     2521 cgaagccggc aagcgccagc gccgccagca ccaaaccggc cgcggcccag cgcaccgtct
     2581 gctccgagcc gccaatcaat accagcagca gcaatccaag caacgtgaaa aagaattgtg
     2641 cgatggcttc gagcgtaaga tccaccacca cgctggcgcc ggcgattccg gctttagcgc
     2701 cgttaaatgt cagaatcctc gcgccgacca gttcaccacc gacttgcatc accggcaaga
     2761 gactgcccac cgcctcgcgc accgagcgcg cccagaaaaa caccagcagc gatttgctcc
     2821 aggtgttacg cagtacgcgc cgccacgcca gtgacgacag cgttaccggc aacaggtgaa
     2881 acaggcttac caccaccatg ccccaaccga cgctcagcag caggtcggat acggctatga
     2941 aaccgtgata gccgatcaga agaatcgcag caaggaggcc tgccagcgcg gcgatggcga
     3001 tggccagttt catttctttt cctcggccac actcatgatt gcgggcagga ccaccagtgt
     3061 gcaaagcagt gcgaacgaca aggcgatggt gagcagcaac cccagaccgg acatgccgcg
     3121 gtgttcggaa aaagccaggg aaccgaaaga agccacagta gtaagcgcac tgaacagtac
     3181 ggcacgcgga gtgctgctgc ggaacagatg ttccacgtcc agcccaccgc gcttgcgcat
     3241 caccagataa atgccgaagg ctatcc
//
LOCUS       DATIOW010000231         3977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1013874, whole genome shotgun sequence.
ACCESSION   DATIOW010000231 DATIOW010000000
VERSION     DATIOW010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013874"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1107
                     /gene="mltB"
                     /locus_tag="VLL03_07730"
     CDS             199..1107
                     /gene="mltB"
                     /locus_tag="VLL03_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HSS47286.1"
                     /translation="MEAFIREMAEMHHFNPRELRRIFSQVKFQPEVLKAISTPATTLP
                     WDEYRALFISPARISDGVKFWDDHEKELGRARQKYGVPEEIIVATIGVETRYGRNTGG
                     YRVMDALTTLAFDFPPRADFFKDELEQYLLFTRDLHIDPLSLKGSYAGAMGMPQFIPS
                     AYRCCAVDFDGDGKANLWTSAADAIGSIANYYQVYGWQEDEPIAVGATVSGDEFLPLL
                     SRDIKPHTPVAELQNIGVTPEQEISGDSLASLLALGEGDDAQYWLALNNFYVITRYNR
                     SVYYAMAVYELGREIRAARGTQIDDK"
     gene            complement(1109..1918)
                     /locus_tag="VLL03_07735"
     CDS             complement(1109..1918)
                     /locus_tag="VLL03_07735"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSS47287.1"
                     /translation="MILRPVYAVIEREVLKLFRQRSRLLSAMVRPMIWLLVIGAGFDA
                     MLGRSGNTSYQYFLVPGVLGMSMLFGAMLAALSTVYDKESGVMRMLVIAPFEHYWIVI
                     AKTLSAALAAIIQAILVLILLALIGYLKNGVAPLLLILGILATSLTCASMGMLIAAWT
                     RTLDNFAVMMNLVIFPVFFLSGSLYPVQHLPSALRFVASLNPYTYGVDLLKHAVLGIN
                     GASVNTDFSVAVDLTVLFGFTLLATMIACLRFSRDTAYEPLIHLLARKRSD"
     gene            complement(1915..2862)
                     /locus_tag="VLL03_07740"
     CDS             complement(1915..2862)
                     /locus_tag="VLL03_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSS47288.1"
                     /translation="MMRDAVSATHAVKAQGLTKRYGQTLAVDALNLEIPAGGFFGLLG
                     PNGSGKTSTIHMLSTLIRPTTGEAWVAGHDVRNDGLKVRAAIGVVFQESALDRTLSVV
                     ENLRFAGLLHNLSLAQIRERSEELLRLFGLTEKRDQAVGELSGGQRRALDIARGVIHQ
                     PRILFLDEPTIGLDVPNRRNIWQFIERLRAEHNMTVFLTTHYLEEAVDCDEVAFIKQG
                     RSVKSGPPHDLIDGLGAYIIEIEAENLKELAQNLSSRLGPSYMDGDKACFRFTGRDLT
                     ALMLLQTELGDRVTGMRWRKPNLNDVFSWVNETEHWDRS"
     gene            complement(2870..3976)
                     /gene="pqqE"
                     /locus_tag="VLL03_07745"
     CDS             complement(2870..3976)
                     /gene="pqqE"
                     /locus_tag="VLL03_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HSS47289.1"
                     /translation="MWLLAEITYKCPLHCVYCSNPIDYTRYGAELSTDDWLRVLRESR
                     ELGATQLGFSGGEPLVRNDLEILVAEARKLGYYSNLITSGIGMNEKRIAAFKEGGLDH
                     IQLSFQDSTRELNDFLGSTRTFDIKMKVAKLIKQYEYPMVLNVVLHRQNIEHIQQILD
                     MAEQFGVEYLELANTQYYGWAMLNRDHLLPSREQLERAEAIVNRFREKIGDKMRIYFV
                     VPDYYENRPKACMNGWGSVFITITADGVVLPCHEARMLPGLTFPNVREADMKWIWYDS
                     PAFNTYRGDEWMKEPCRSCPEKTKDFGGCRCQAYMLTGDARNADPVCDLSPLHHVVMD
                     AVAKAQQAQAKTVAVRPIMFRDDHNSRNLSKSAA"
BASE COUNT          843 a         1134 c         1179 g          821 t
ORIGIN      
        1 ctggggctcg ctgatgggcg cgtgcctgat tgtgagagtt cccatcgcca tcctgtataa
       61 tttcttcgtc gacctctttg tagcgggctt taccgtggga gctgttaaat aatcgcaagc
      121 gcaaaacgtt tcgccttcct aatttgcatc gcgctgtttt ttccgggcaa cagccatgcc
      181 gccccgctga atccagacat ggaggccttc atccgcgaga tggcggaaat gcatcatttc
      241 aatccacggg agttgcgccg aatcttcagc caggtcaaat ttcagcccga ggtgctcaag
      301 gcgatttcca ctcccgcgac gacgctgccc tgggatgagt accgcgcgtt gttcatcagt
      361 ccggcgcgca tctccgatgg cgtcaaattc tgggacgatc acgaaaagga actcgggcgc
      421 gccaggcaga aatacggcgt gccggaagaa atcatcgtcg ccaccatcgg cgtggaaacg
      481 cgttacggca ggaacaccgg cgggtaccgc gtgatggacg cgctcaccac gctggcgttt
      541 gatttcccgc cgcgcgcgga ttttttcaag gatgaactcg aacagtacct gttgtttaca
      601 cgggaccttc acatcgatcc gttgagcctc aagggctcct acgccggggc aatgggcatg
      661 ccgcaattca ttcccagcgc ttaccgctgc tgcgcagtag attttgacgg cgacggcaaa
      721 gccaatctct ggacaagcgc tgccgatgcc atcggcagca tcgccaatta ttaccaggtt
      781 tacggctggc aggaggatga gccgatcgcg gtgggcgcga cggtgagcgg cgatgaattc
      841 ctgcccttgc tgtcacggga cattaagccg cacacgccag tggccgaatt gcaaaacatc
      901 ggcgttaccc ccgagcagga aatttccgga gacagtctgg cgagcctgtt ggccttaggg
      961 gagggtgatg atgcgcagta ctggctcgcg ctcaataatt tttatgtgat tacccgctac
     1021 aaccgcagcg tctattacgc gatggcggtg tatgaactgg gccgcgagat ccgcgccgcc
     1081 aggggaacgc agatagatga taaataaact agtctgaacg cttgcgagca agcagatgta
     1141 tcagcggctc gtaggcggtg tcgcgcgaaa agcgcaaaca tgcaatcatc gtggcaagca
     1201 aggtgaagcc gaacaacacc gtcagatcaa ccgccacgct gaaatccgtg ttaactgacg
     1261 cgccgttaat tcccagcacg gcgtgcttga gcaaatccac gccataggta taagggttga
     1321 ggctggcaac gaagcgcagc gcgctcggaa ggtgctgcac cggataaagc gagccggaga
     1381 gaaagaacac cgggaaaatc accaggttca tcatcaccgc gaagttatca agcgtgcgcg
     1441 tccacgcggc aatcagcatg cccatgctgg cgcaggtgag cgaagtggcg aggatgccga
     1501 gaatcaacag cagcggcgcc acgccgtttt tgagatagcc aatgagcgcg agcaatatca
     1561 gcaccagtat ggcttgaatg atggcggcaa gcgccgcgct caaggtcttg gcaatcacaa
     1621 tccagtaatg ctcgaacggc gcgatgacca gcatgcgcat cacgcctgat tccttgtcgt
     1681 agacggtgga aagcgcggcg agcatggcgc cgaacagcat ggacatgccg agcacgccgg
     1741 gcacgaggaa atattgatag ctggtgttgc ccgaacgtcc cagcatggcg tcgaatccgg
     1801 cgccaatcac cagcagccag atcatcggcc gcaccatggc ggaaagcagg cgcgagcgct
     1861 ggcggaagag cttcagcact tcgcgttcga ttacggcgta tacagggcgc aatatcatga
     1921 ccgatcccag tgttcggttt cattcaccca cgaaaacaca tcgttgagat tgggtttgcg
     1981 ccaacgcata ccggtcaccc tgtcgcccag ttcagtttgc agcagcatca gcgccgtcag
     2041 gtcgcggccg gtaaagcgga agcaggcctt gtcgccatcc atgtagctcg gccccaggcg
     2101 ggaactcagg ttttgtgcca gctccttcag gttctcagct tcgatttcga tgatgtaggc
     2161 gccgagaccg tcaatcaaat cgtgaggcgg gccgcttttg acgctacgcc cctgtttgat
     2221 aaacgctact tcgtcgcaat ccactgcttc ttcaagatag tgggtggtga gaaacaccgt
     2281 catgttgtgc tccgcgcgca gccgctcgat gaactgccag atattgcgtc ggttcggcac
     2341 atcgagcccg atggtcggct catcgagaaa aagaatgcgc ggctggtgaa tcacgccgcg
     2401 ggcgatatcg agcgcgcggc gctggccgcc ggagagttcg cccactgctt gatcgcgttt
     2461 ttcagtcagc ccgaaaagcc gcagcaattc ttcgctgcgc tccctgattt gcgccaatga
     2521 cagattgtga agcagtccgg caaagcgcag gttttcaacg acggagagag tgcggtctag
     2581 cgcggattcc tgaaacacca cgccgatggc ggcgcgcacc ttgaggccgt cgtttctcac
     2641 atcatgcccc gccacccagg cctcgcctgt tgtcgggcgg atgagcgtgg agagcatgtg
     2701 gatggtgctg gttttccccg agccgttggg gccgagcagg ccgaagaaac caccagcggg
     2761 tatttccaga ttcagtgcat caaccgcgag cgtctgaccg taacgcttgg taagtccttg
     2821 tgccttgact gcgtgcgttg cggaaacggc gtcgcgcatc attggactat caggcggcgc
     2881 tcttgctcaa attgcgggag ttgtgatcgt cgcggaacat gatcggccgc accgcgacgg
     2941 ttttggcttg cgcttgctgc gccttggcca cggcgtccat gaccacatga tgcagcggcg
     3001 agagatcgca caccgggtcg gcgttgcgtg catcccccgt gagcatgtag gcttgacagc
     3061 ggcagccgcc gaagtccttg gttttttccg ggcaggagcg gcacggctcc ttcatccatt
     3121 cgtcgccgcg ataagtattg aatgccggcg aatcgtacca gatccatttc atgtccgctt
     3181 cgcgcacgtt ggggaaggtc aagcctggca gcatgcgcgc ttcatggcag ggcagcacca
     3241 ccccgtcggc ggtgatggtg atgaacaccg agccccagcc gttcatgcat gccttggggc
     3301 ggttctcgta atagtcgggc accacgaagt agatgcgcat cttgtcgcct atcttttcgc
     3361 ggaagcggtt cacgatggcc tcggctcgct ccagttgctc gcgcgagggc aaaagatgat
     3421 cgcggttaag catggcccag ccgtaatact gagtgttggc gagttccaga tattccacgc
     3481 cgaactgttc cgccatatcg aggatttgct ggatatgctc gatgttctgc cggtgcagca
     3541 ccacgttcag caccatcgga tattcgtatt gcttgatgag cttcgccact ttcatcttga
     3601 tgtcaaaggt gcgggtgctg cccagaaagt cgttaagctc gcgcgtcgaa tcctggaacg
     3661 agagttggat gtgatcaagc ccgccttcct taaaggcggc gatgcgcttt tcattcatgc
     3721 cgatgccgga agtaatcaga ttgctgtagt agccgagctt tctggcttcc gcaaccagga
     3781 tttccaaatc attgcgcacc agcggctcac cgccggaaaa gcccagttgg gttgcgccca
     3841 actcgcgcga ttctcgcagc acgcgcagcc agtcatcggt cgagagctcc gcgccgtagc
     3901 gcgtgtaatc aatggggttg gagcaataca cgcagtggag cgggcattta taagtgattt
     3961 ccgccagcag ccacagc
//
LOCUS       DATIOW010000232         5681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_811961, whole genome shotgun sequence.
ACCESSION   DATIOW010000232 DATIOW010000000
VERSION     DATIOW010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811961"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /gene="ribB"
                     /locus_tag="VLL03_07750"
     CDS             complement(<1..674)
                     /gene="ribB"
                     /locus_tag="VLL03_07750"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009921779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HSS47290.1"
                     /translation="MSAGISPIQDLIADVRAGRMVILVDEEDRENEGDLLLAAEFATP
                     EAINFMAKHGRGLICLTLTEERCRQLNLPLMVVQNRSSLGTNFTASIEAREGVTTGIS
                     AADRARTIQVAVKKDANPEDIAQPGHVFPLMAQQGGVLARAGHTEAGCDLAQLAGLEP
                     AAVICEVLKDNGEMARLADLLEFARQHQLKIGTIADLIHYRSRTESLVTRVLERMVDT
                     PHGKFRL"
     gene            complement(671..1261)
                     /locus_tag="VLL03_07755"
     CDS             complement(671..1261)
                     /locus_tag="VLL03_07755"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HSS47291.1"
                     /translation="MFSGIVAAVGEVSQVNSLAGGAHVVISSGLDLSDVKQGDSIAVN
                     GVCLTVTGLGRGAFKADVSRETLNCTSGFAQKDKVNLEKALRLSDRVNGHLVSGHVDG
                     VGKVAKFERTGECYLLAVKAPRGLAKYISRKGSIAVNGVSLTVNKVEGGEFSVNLIPH
                     TLASTNLRYLKRGAKVNLEADMLARYAEQLLMTKRK"
     gene            complement(1246..2007)
                     /locus_tag="VLL03_07760"
     CDS             complement(1246..2007)
                     /locus_tag="VLL03_07760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSS47292.1"
                     /translation="MGGFKDHFSGHAAKYAQFRPHYPSGLFACLASLVSSRELAWDAA
                     TGNGQGALDLAPYFQRVVGSDASRAQIENAIPHSKVCYRIEPAEATQFATASVDLISV
                     AQALHWLDLPKFYAEVKRVLKAEGVIAVWCYNLFRAAPAIDNIILRFYHDLVGPHWPP
                     ERAHIETGYRHLEFPFAEINAPLFVMEVEWDMHELLGYLGTWSATQRYIKAEGHDPRW
                     HIAGRLESVWGSPQNKRRIQWPLYLKVGKQPCSAA"
     gene            complement(2013..3098)
                     /gene="ribD"
                     /locus_tag="VLL03_07765"
     CDS             complement(2013..3098)
                     /gene="ribD"
                     /locus_tag="VLL03_07765"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HSS47293.1"
                     /translation="MFSPADHEFMAQALRLAEKGLYSTSPNPRVGCVMVRAGKIIGEG
                     WHEKAGGPHAEILALNRAGEEARGATAYVTLEPCNHQGRTGPCTEALIKAAVARVVAA
                     VQDPNPLVNGAGTTKLKAAGIQVESGLLENEARELNIGYVSRMIRSRPWVRMKIAASL
                     DGKTALNNGQSKWITGEAARRDAHHWRARSCAVLTGIGTVKDDDPQLNVRHVSTPRQP
                     VRVVVDSRLEISPSAKVLQGGKVVIFCAVNDGQRAARLREKDVQLIALPNSEGKVELS
                     QMMAHLAKLEVNEVLVEGGFKLNGSLLQAGLVDELLIYFAPRLIGDNARGMFHLSELG
                     DLAGRRELKILDLRKVGEDMRVLARPA"
     gene            complement(3111..3596)
                     /gene="nrdR"
                     /locus_tag="VLL03_07770"
     CDS             complement(3111..3596)
                     /gene="nrdR"
                     /locus_tag="VLL03_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HSS47294.1"
                     /translation="MKCPFCNAQDTQVVDSRVNEEGTSVRRRRRCVSCMKRFTTYETV
                     ELRLPQIIKHNGTRTEFDRNRLHTGFMRALHKRPVSAERVDEAIDRVTQKLLSLGERE
                     VPSRLIGELVMKELYKMDKVAYIRFASVYRSFQDVEDFRDVIEEVQQPAKNRSRRRKA
                     G"
     gene            complement(3657..4928)
                     /gene="glyA"
                     /locus_tag="VLL03_07775"
     CDS             complement(3657..4928)
                     /gene="glyA"
                     /locus_tag="VLL03_07775"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HSS47295.1"
                     /translation="MSFSTQQNLAKIDPELWQAICAENQRQEDHIELIASENYASPAV
                     LQAQGTVLTNKYAEGYPGKRYYGGCEFVDIAEQLAIDRIKALFNAQYANVQPHSGSQA
                     NQGVYFAMLKPGDTILGMSLAHGGHLTHGAAVNLSGKIFNAVTYGLHPKTEEIDYDEV
                     QRLAKQHKPKMIIAGASAYALVIDWKRFREIADSVGAYLFVDMAHYAGLVAAGYYPNP
                     TGIADFVTSTTHKTLRGPRGGFILCNTEEHAKAINSTIFPGIQGGPLMHVIAAKAVAF
                     KEAMSKEFKQYQKLVIDNARVMTKVLQKRGVRIVSGRTDCHLFLVDLRAKKITGKDAE
                     SALGRAHITVNKNAIPNDPQKPFVTSGVRIGTPAMTTRGFQEIEAEQLSNLIADVLEA
                     PNDQKVIARVAGEVKTMCAKFPVYGSKGVRS"
     gene            complement(5064..>5681)
                     /locus_tag="VLL03_07780"
     CDS             complement(5064..>5681)
                     /locus_tag="VLL03_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HSS47296.1"
                     /translation="VLFEAPVAASLLGHFVAAASGGSLYRKSSFLLDSMGKQIFAPLV
                     QIRELPHMKKGLASSAFDDEGVATRERDVVKDGVLQGYFLGSYSARKLGMQSTGNAGG
                     NHNLILESGGLDLNGLLKKMGKGLLVTELLGHGVNMVTGDYSTGAAGFWVEHGEIQYP
                     VQEITIAGNLKDMFRGIVAVGGDMLVRGSRQCGSILIDNMTVAGD"
BASE COUNT         1125 a         1757 c         1553 g         1246 t
ORIGIN      
        1 agcctgaatt tgccgtgcgg cgtatccacc atgcgttcca acacgcgcgt caccagcgat
       61 tcggtgcggc tgcggtaatg aatcaaatcg gcgatggtgc cgattttgag ttgatgctgc
      121 ctggcaaatt cgagcaaatc ggcaagccgc gccatttcgc cgttgtcttt cagaacttcg
      181 cagatcaccg ccgccggctc caaaccggca agctgcgcca gatcgcagcc cgcctcggta
      241 tgcccggcgc gcgccaatac cccgccctgc tgcgccatta gggggaaaac atgtcccggc
      301 tgggcgatgt cttcagggtt agcgtctttt ttcaccgcca cctggatggt acgcgcccgg
      361 tcggcggcgg aaatgccggt agtcacgcct tcgcgcgctt cgatcgaggc ggtaaagttg
      421 gtgccgagcg acgaacggtt ttgcaccacc atcaagggca gattaagttg acggcagcgc
      481 tcctcggtca aggtcaggca aatcaggccg cggccgtgct tggccataaa gttgatggcc
      541 tcgggcgtgg caaattcggc ggccagcaaa agatcgcctt cgttctcgcg gtcctcttcg
      601 tcgacgagaa tcaccatccg gccggcgcgc acatccgcga tgaggtcctg aatcggagaa
      661 atacctgcgc tcatttgcgt tttgtcatca gaagctgttc ggcgtaacgc gccagcatgt
      721 ccgcttccaa gttcactttg gcgccgcgct tcaaatatct caggttggtc gaggctagcg
      781 tgtgcggaat caggttgact gagaattcgc cgccctcgac cttgtttacc gtcaggctca
      841 cgccatttac cgcaatcgaa cctttgcgcg agatgtattt ggcgagcccg cgcggcgcct
      901 tgacggccag caaataacac tcgcccgtcc gttcaaactt tgcaaccttg cccacgccgt
      961 ccacatggcc gctcaccagg tgaccgttga cgcggtcgga tagccgcagc gctttttcca
     1021 gattgacctt gtccttttgc gcgaaccccg aagtgcaatt cagtgtttcc cgcgagacat
     1081 ccgctttgaa cgcgccgcgc ccaagcccgg ttaccgtaag acaaacaccg ttaacggcga
     1141 tgctgtcgcc ttgcttcaca tcgctcaaat ccagcccgct ggagataacg acatgcgcgc
     1201 ctccggccag ggaattgacc tggcttactt caccaaccgc tgcgactatg ccgctgaaca
     1261 tggctgcttg cccactttaa gatacagcgg ccattgtatg cgccgcttgt tctgcgggga
     1321 accccacacc gactccagcc tcccggcaat gtgccagcgc gggtcatgtc cctcggcctt
     1381 gatgtagcgc tgcgtcgccg accaggtgcc aagatagccc agaagctcat gcatatccca
     1441 ctctacttcc atcacgaaga gaggcgcatt gatttcggca aagggaaatt cgagatgccg
     1501 gtagccggtt tcgatatgcg cgcgttccgg cggccaatgg ggcccgacga ggtcatgata
     1561 aaaacgcaag atgatattat cgatagcggg cgcagcgcgg aacaagttgt agcaccacac
     1621 cgcaatcacg ccctccgcct tcagcacgcg tttgacttcg gcatagaatt tcggcaaatc
     1681 cagccagtgc agcgcctgcg ctacgctgat taaatccacg cttgcggtcg caaactgcgt
     1741 tgcttcagcc ggctcgatcc ggtagcaaac tttggaatgc ggaatcgcgt tttcaatctg
     1801 cgcgcggctt gcgtcactgc caaccactcg ctggaaataa ggcgccaaat ccagcgcccc
     1861 ctgcccgttg ccggtcgccg catcccaggc cagttctcga gaggatacta gcgaagcgag
     1921 acaagcgaac agcccggaag gataatgcgg ccggaactgc gcatatttgg cggcatggcc
     1981 ggagaaatga tccttgaacc cgcccatagg tttcaagccg gacgcgccag cacgcgcatg
     2041 tcttcgccca ccttgcgcag atcaaggatc ttaagttcgc gccggccggc gaggtcaccg
     2101 agttcggaaa gatgaaacat gccgcgtgca ttgtcgccaa tcagacgcgg cgcgaaataa
     2161 atcaacaact catccaccag ccccgcttgc aacagcgagc cattgagctt gaagccaccc
     2221 tccaccagca cttcattcac ttccagcttc gccagatgtg ccatcatttg agaaagctcg
     2281 accttgccct cgctattggg cagggcaatc agctgcacgt ctttctcgcg cagccgggcc
     2341 gctctttgcc cgtcattcac cgcacaaaaa atcacaacct tgccgccttg cagaactttg
     2401 gccgaaggag aaatctccag ccggctgtcc accacgacgc ggaccggctg ccgcggggtg
     2461 ctcacatgtc gcacattcaa ttgcgggtca tcgtctttaa ccgtgccgat gccggtcaac
     2521 accgcgcagg aacgcgcgcg ccagtggtgc gcgtcgcgcc gcgcggcttc gcccgtaatc
     2581 catttacttt ggccgttgtt gagcgcggtc ttgccgtcca ggcttgcggc gattttcatg
     2641 cgaacccagg ggcgactgcg gatcatacgc gaaacatagc caatgttgag ttcgcgcgct
     2701 tcgttttcca gcaaaccgct ctcaacctgg atgccggcgg ctttaagctt ggtggtgccg
     2761 gcgccattga ccaggggatt gggatcctgc accgccgcca ccactcttgc cactgcggct
     2821 ttaatcaaag cttcggtgca cggccccgtg cgcccctggt ggttgcacgg ttcgagggtc
     2881 acatatgcgg ttgcgccgcg cgcttcctct cccgcgcgat tgagcgcaag gatttcagca
     2941 tgcggaccgc cggctttttc atgccagccc tcgccgataa ttttgccggc gcgcaccatc
     3001 acgcaaccta cgcgcgggtt gggactggtg ctgtacaaac ctttttcagc gaggcgcagc
     3061 gcctgcgcca tgaactcatg atctgcggga gaaaacatag aaaaaactgt ttaacccgcc
     3121 ttgcggcggc gtgagcggtt tttggcgggt tgctgcactt cttcaattac gtcgcgaaaa
     3181 tcctcgacat cctggaagct gcggtacacc gacgcgaaac gaatgtaggc gaccttgtcc
     3241 attttataga gttccttcat caccaattcg cctatcagcc gtgacggcac ttcgcgctcg
     3301 cccaggctca acagcttttg cgtcacccgg tctatcgctt catccacccg ctcggccgaa
     3361 accggccgct tgtgcagcgc gcgcatgaag ccggtatgca gccggttgcg gtcgaattcg
     3421 gtgcgcgtac cgttgtgctt gatgatctgc ggcaggcgca attccacggt ttcgtaagtg
     3481 gtaaaccgct tcatgcacga aacgcagcgt cggcgccggc gcacgctggt gccttcctcg
     3541 ttcacccgcg aatccaccac ctgggtgtct tgcgcattgc agaaagggca tttcatgatt
     3601 tgcggtaagg agtaaggcgt gaggtgtgaa gggtgtaagt cagcatcttt ttactcctaa
     3661 ctcctcaccc ccttgcttcc atagaccggg aatttggcgc acatcgtttt cacttctccc
     3721 gccacgcgtg caatgacttt ctggtcgttc ggcgcttcaa gcacgtcggc gatcaggttc
     3781 gacaactgct cggcttcgat ttcctggaag ccgcgcgtgg tcatagccgg cgtgccgatg
     3841 cgcacgccgc tggtgacgaa aggcttctgc ggatcgttgg gaatggcatt cttgttgacc
     3901 gtgatatgcg cccgccccag cgcgctttca gcgtccttgc cggtgatttt cttggcgcgc
     3961 agatccacca ggaacagatg gcagtcggtg cgccccgaga caatgcgcac gccacgtttt
     4021 tgcagcacct tggtcatcac ccgcgcgttg tctatcacca gcttctggta ttgcttgaat
     4081 tccttgctca tcgcttcctt gaacgcaacc gccttggcgg caatcacgtg catgagcggc
     4141 ccgccctgaa tgccggggaa aatggtggaa ttaatcgcct tggcgtgttc ttccgtgttg
     4201 cacaggatga acccgccgcg cggtccgcgc agcgtcttgt gcgtagtgct ggtgacaaaa
     4261 tcggcgatgc ccgtcggatt gggatagtag cccgccgcga ccaatccggc ataatgcgcc
     4321 atgtccacga acaaataagc gcccaccgaa tcggcgattt cacggaaacg cttccagtcg
     4381 attaccagcg cataggctga ggcgccggcg atgatcattt tcggtttgtg ctgtttcgcc
     4441 agccgttgca cttcgtcgta gtcaatttcc tcggttttgg gatgcaagcc ataagtaacg
     4501 gcgttgaaga ttttgccact gagattgacc gctgcgccat gcgtgagatg cccgccatgc
     4561 gccagcgaca tgcccaatat ggtgtcgccg ggcttgagca tggcaaagta aaccccttga
     4621 ttagcctgcg agccggaatg cggctgcaca ttggcatact gcgcgttgaa tagtgctttt
     4681 atgcggtcaa tcgccagttg ttcggcgatg tcaacaaact cgcagccgcc gtagtagcgc
     4741 ttgccgggat agccctcggc atacttgttg gtgagcaccg taccctgcgc ctgcaatacg
     4801 gccgggctgg cataattttc cgaggcaatc agctctatat gatcttcctg gcgctggttt
     4861 tcggcgcaaa tcgcctgcca cagttccgga tcgattttgg cgagattctg ttgagtagaa
     4921 aaggacataa gcggctttct tataacagtc aattaaaagt ttgattttaa catggcagac
     4981 cgggctgcgc gcccccggcc ttttgtggat aaatcctgca ccgaaaaccg cccgtctgca
     5041 gaaaaccgcg tttgacgccc gcctcaatcc ccggcgacag tcatgttatc tataagaata
     5101 gagccgcact gccgcgagcc gcgcactaac atatccccgc ctacagccac aatgccgcgg
     5161 aacatgtcct tgaggtttcc ggcaatggtg atttcctgta ccgggtactg gatttctccg
     5221 tgttccaccc agaaacccgc ggcaccggtg gagtaatcgc ccgtcaccat gtttacaccg
     5281 tgccccagca attccgtgac cagcaagccc ttgcccattt ttttgaggag accgtttaag
     5341 tccagccccc cgctttcgag aatgagatta tggttgccgc cggcattgcc ggtggattgc
     5401 atgcccagct tgcgcgctga gtagctgccg agaaaataac cttgcaggac gccgtctttc
     5461 accacgtcgc gctctcgtgt cgctactcct tcgtcgtcaa acgcgctgct ggcaaggccc
     5521 tttttcatat gcggcaattc cctgatttgc accaaaggcg cgaagatttg cttgcccatg
     5581 ctgtcgagca gaaaagacga tttgcggtac aggctgccgc cgcttgccgc cgcaacgaaa
     5641 tggccgagca ggctcgccgc aaccggcgcc tcgaacagca c
//
LOCUS       DATIOW010000233         2509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U15834
            k141_1014663, whole genome shotgun sequence.
ACCESSION   DATIOW010000233 DATIOW010000000
VERSION     DATIOW010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37936545
            Sequence Read Archive: SRR9656166
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 07:59:28
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,559
            CDSs (total)                      :: 1,537
            Genes (coding)                    :: 1,510
            CDSs (with protein)               :: 1,510
            Genes (RNA)                       :: 22
            tRNAs                             :: 20
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 6 of 27
            Pseudo Genes (incomplete)         :: 21 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            Pseudo Genes (multiple problems)  :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014663"
                     /isolate="SMAG_U15834"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="New Zealand: Waikato"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="VLL03_07785"
     CDS             <1..1314
                     /locus_tag="VLL03_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSS47297.1"
                     /translation="KKSAWKITVPRLKNASVKPMRFDLAALRKEISRTAGLPVVLAIH
                     TNSRTLAFAQRKDVAKISQQGPATPDHVIRTKRIPMVGRDVAAYSKSYKEYFHQYVRG
                     AREKKTMLDPAPRVILDSEFGLGAVGQTVKDAAIVAEIYLHTIEVILRATALGGFHAL
                     PAKELFEVEYWDLEQAKLKKGGKPPVFAGEIALVTGAASGIGKACTDSLLARGAAVIG
                     LDLNPAIESLYQRSDFLGLRCDVTSEKAAAGALEAGARAFGGLDMLVLNAGIFPTSRR
                     IEALKTDEWRKVMNVNLDANLMLMRECHPLLKLAPKGGRVVVIGSKNVPAPGPGAGAY
                     SASKAALNQLARVAALEWGANNIRINTLHPNAVFDTAIWSDEVLASRAAQYGLSVEQY
                     RKNNVLKTEVSSRDVAELAAEMCGPLFAKTTGAQVPVDGGNERVI"
     gene            1314..2360
                     /gene="mtnA"
                     /locus_tag="VLL03_07790"
     CDS             1314..2360
                     /gene="mtnA"
                     /locus_tag="VLL03_07790"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HSS47298.1"
                     /translation="MQTDKIETLRWRDNRLEMIDQRILPERFEYLSFDSAAAVADGIR
                     NMVVRGAPAIGCAAAYGVALEALRLKHLPANEFEAALEQGFRLLAGSRPTAVNLFWAL
                     TRMRETWNSVKGRQPAVIAEKLLSEAQAIQAEDIRINRAIGRHGVELIKNEARILTHC
                     NAGALATAGYGTALGVIRSAMEAGKKVSVVVGETRPFLQGARLTAWELVQEQIPATLI
                     TDNMAGHLMSRGEINVIIVGADRVAANGDVANKIGTYMLAVLAYRHRIPFYVACPLSS
                     IDLNLANGKDIPIEERSAEEVSGYREMQWAAPGVSIRNPAFDVTPAELVTGLITEKGV
                     VLSPNKEKISKLFA"
     gene            complement(2367..>2509)
                     /locus_tag="VLL03_07795"
     CDS             complement(2367..>2509)
                     /locus_tag="VLL03_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSS47299.1"
                     /translation="RAMEEKVPLGRLGTPEEIANTYAFLASDEASYINGAVIEVSGGM
                     TL"
BASE COUNT          566 a          713 c          753 g          477 t
ORIGIN      
        1 aaaaaaagcg cgtggaaaat caccgtgcca cgcctcaaga atgcttccgt caagccgatg
       61 cgattcgacc tggctgcgct gagaaaagag atctcgagaa ccgccggctt gccggtggtc
      121 ctcgccatcc acactaacag ccggactctg gcctttgctc aacgcaaaga tgtggcgaaa
      181 atttcccagc aaggcccggc cacgcccgat catgtgattc gtaccaagcg catcccgatg
      241 gtgggaagag acgttgcagc ctattccaaa tcctacaagg aatacttcca tcagtatgtg
      301 cgcggagcca gggaaaagaa gaccatgctc gatcccgcgc cgcgggtgat tctggactcc
      361 gaattcggct tgggcgcggt ggggcagacg gtgaaagatg cggcaatcgt cgccgaaatt
      421 tacctgcaca cgattgaagt cattttgcgc gcgacggctt taggcgggtt tcacgctctt
      481 ccggcaaaag aattatttga agtcgagtac tgggacctcg aacaggcgaa actgaagaaa
      541 ggcggcaagc ctccggtgtt tgccggcgaa atcgcgttgg tgaccggcgc cgcttccggc
      601 ataggcaagg cctgcacgga ctcgctactg gctcgcggcg ccgcggtcat cgggctggat
      661 ttgaatccgg cgattgaatc gctttaccag cgcagcgatt ttctggggtt gcgttgcgat
      721 gtcaccagcg aaaaagcggc ggcaggagcg ctggaggccg gtgccagggc attcggcggg
      781 ctcgacatgc tggtactcaa tgccggaatt ttccctacca gccgccgcat cgaagccttg
      841 aaaacagatg aatggcgcaa ggtgatgaac gtcaatctcg acgccaacct gatgttgatg
      901 cgcgaatgcc atcccctgct caagctcgcc cccaagggcg ggcgggtggt ggtcatcggc
      961 tcgaaaaatg tgcccgcgcc cggtcccggt gccggcgcct attccgcgtc caaggcggcg
     1021 ctcaatcaat tggcgcgggt cgcggcgctg gaatggggag cgaataacat acgcattaat
     1081 accctacacc ccaatgccgt ttttgatacc gccatctgga gcgatgaggt gctggcttcg
     1141 cgcgcggccc aatacggcct cagcgtagag caatacagga aaaacaacgt gctcaagaca
     1201 gaagtcagca gccgcgatgt ggcggaactg gcggcggaaa tgtgcggccc actgtttgcc
     1261 aagactacag gcgcgcaagt tcccgtagac ggcggcaatg agcgggttat ttgatgcaaa
     1321 ccgacaaaat tgaaacgctg cgctggcgcg ataaccgcct ggagatgatt gaccagcgca
     1381 tcctgcccga acgctttgaa tacttgagtt tcgattccgc cgccgccgtc gccgacggca
     1441 tccgtaacat ggtggtgaga ggcgcgcccg ccatcggttg cgccgccgcc tatggtgtcg
     1501 cgcttgaagc gctgcgactg aaacatctac cggcaaatga attcgaagcg gcattggaac
     1561 agggtttccg cctgctcgcg gggagccgcc ctaccgcggt gaatctgttt tgggcgctca
     1621 cgcgcatgcg cgaaacctgg aattcggtca aaggccggca acccgcagtg attgcggaaa
     1681 aactgctgag cgaagcacag gccatccaag ccgaagacat ccgcatcaat cgcgccatcg
     1741 gcagacacgg agttgaactt ataaaaaatg aggccagaat tcttacccat tgcaacgccg
     1801 gtgcgctggc cactgccgga tacggcaccg cgctgggtgt gatccgctcc gccatggaag
     1861 caggcaaaaa agtttcggtg gtggtaggcg aaacacgccc tttcctgcaa ggcgcaaggc
     1921 tcaccgcctg ggaactggtt caggagcaga ttcctgcgac gctgattacc gacaatatgg
     1981 cgggacacct gatgagccgc ggggaaatca atgtgatcat cgtcggcgcc gaccgggtgg
     2041 cggccaacgg cgatgtggcg aacaaaatcg gcacttatat gctggcggtg ctggcatacc
     2101 gtcaccgcat tcctttttat gtcgcctgcc cgctctctag cattgatctc aacctggcaa
     2161 acggcaaaga catcccgata gaagaacgct cggctgaaga agtaagcggt tatcgcgaaa
     2221 tgcagtgggc ggcgccaggg gtgagtatcc gcaacccggc gtttgatgtg acccccgcgg
     2281 aactggtgac gggattgatc accgaaaaag gcgtggtgct ctcccccaac aaggaaaaaa
     2341 tcagtaagct ttttgcttga agcggcttaa agcgtcatgc cgccgctgac ttcgattacc
     2401 gcgccgttga tgtagctcgc ctcgtcggag gcgaggaagg cataggtgtt ggcgatttct
     2461 tccggcgtgc cgagacgacc caaaggcact ttctcttcca tcgctctca
//
